LOCUS       DATNDD010000001         4614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2584925, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000001 DATNDD010000000
VERSION     DATNDD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584925"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..327)
                     /gene="pqqD"
                     /locus_tag="VN647_00005"
     CDS             complement(34..327)
                     /gene="pqqD"
                     /locus_tag="VN647_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HWG95435.1"
                     /translation="MTLTTVKPRLSFKVRLRFDGRTGRHLLLYPERGLELNDTATVIA
                     RLCTGEWTIDEMVDHLTRAYDAVSPTEIRREVCEFLDVLADRGLLQIGGGKLG"
     gene            complement(296..1090)
                     /gene="pqqC"
                     /locus_tag="VN647_00010"
     CDS             complement(296..1090)
                     /gene="pqqC"
                     /locus_tag="VN647_00010"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HWG95436.1"
                     /translation="MGRNGDPVVMMKEDHHRDRLSQDAFIEWLKREGSRRYHDRHPFH
                     ERMHNGNLTKTQLQQWVLNRYYYQTRIPIKDALIVSKSEDPTFRRMWLRRIRDHDGEQ
                     EGEGGLALWLELARGVGLDAEEARSFRYVLPGVRLACDDYVRFVREAPLLDAVASSLT
                     ELFAPSLMARRLEAWKQHYPWVCSESLEYFQVRISRAALDSNQAIEFVVRHATTYSDQ
                     ARCVRALVKKADILWTMLDHLSMAYVETGTEGKQAAHDSYHSQTSA"
     gene            complement(1063..1986)
                     /gene="pqqB"
                     /locus_tag="VN647_00015"
     CDS             complement(1063..1986)
                     /gene="pqqB"
                     /locus_tag="VN647_00015"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HWG95437.1"
                     /translation="MLIRVLGSAAGGGFPQWNCACVNCKGMRKGLIAASPRTQESISF
                     STGDGNWFLINASPDIRAQIESFGSLHPRQSRSTPIQAVFLTNGDLDHCLGLLSLREN
                     HRLVLYATDSVRRGFTEGNVLYRTLQRFAGQVTWRTLKLDVEEPVLLADGRPSGLTVT
                     AVAVPGKPPIHLEGLVSSSETDVNVGLRFRQSTNGGVLVYFSAVGRITSSVLNALEGA
                     GCVMFDGTFWSSDELSVPGFLAKSAEDLAHCPVGGPEGSLAMLAKIAASRRVFIHINN
                     TNPMLREDSHERKLVEAAGWEIAWDGMEILL"
     gene            complement(2002..2178)
                     /locus_tag="VN647_00020"
     CDS             complement(2002..2178)
                     /locus_tag="VN647_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95438.1"
                     /translation="MIDIFLIIEGGTIMNWERPSFVEIKMDAEINSYQDDFRDGPDAK
                     DPSTETPVITTRQE"
     gene            2571..>4614
                     /gene="glgX"
                     /locus_tag="VN647_00025"
     CDS             2571..>4614
                     /gene="glgX"
                     /locus_tag="VN647_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWG95439.1"
                     /translation="MRIWPGHPFPLGADWDGQGVNFALFSENATKVELCLFDRADDPK
                     PSLCLPLEEQTNQVWHTYLPELFPGQHYGYRVHGPYDPKQGHRFNPAKLLIDPYAKAI
                     AGNVQWTEGRMFGYRIGDPEADLSMDDRDNAADVPKSVVVDSAFTWGADKLPRTAWDE
                     TVIYEVHVKGLTARHPEVPEAQRGTYLGMTSPAVLDHLKTLGVTAVELLPVHHFVRDQ
                     HLHDRGLTNYWGYNSIGFFAPDIRYAYSQKRGEQVREFKTMVKTLHSEGIEVILDVVY
                     NHTAEGNHLGPTICFRGIDNAAYYRLVDDNKRYYMDYTGTGNTLNMNHPRVLQLIMDS
                     LRYWVEEMHVDGFRFDLASTLARELHDVNRLGAFFDIIYQDPVLSRAKLIAEPWDLAS
                     GGYQVGNFPLGWAEWNDKYRDTIRRYVKGDGGQVSELGYRISGSSDLYERSGKRPYAS
                     INFVTAHDGFTLDDLVSYNEKHNEANGEENRDGNDNNQSWNCGAEGPTNDPAIMALRD
                     RQKRNFLAILLLSQGVPMLCGGDELGRTQGGNNNAYCQDNEISWFDWDLKDRDRQLLA
                     FVRRLVQLRHEQPVLRRRQFFQGRRIRGSEIKDIAWFRPDGHEMTDQDWQDAHVRSLG
                     VRLAGDAIQEKDYKGHSVYGDTLLLLFNANHEPLSFTLPAHQRGVRWEILLDTSDPE"
BASE COUNT         1054 a         1317 c         1251 g          992 t
ORIGIN      
        1 accgtacgga taattcaagt catattgttg gcatcaaccg agttttccgc cgcctatttg
       61 aagcaggccg cgatcggcaa ggacgtccag aaactcgcac acctctcgcc tgatctcggt
      121 aggagacacg gcatcatagg ctcgggtgag atgatccacc atctcatcga tcgtccattc
      181 acccgtgcac agacgcgcaa tcaccgtggc cgtgtcgttc agttcgagtc ccctttcggg
      241 gtacaagagc aggtggcgac cggtacgtcc atcgaaccga aggcgaactt tgaaactaag
      301 ccgaggtttg actgtggtaa gagtcatgag cagcttgctt tccttctgtt cccgtttcga
      361 catatgccat agagaggtga tcgagcatgg tccacaaaat gtcggccttc ttgaccaagg
      421 ccctcacaca tcttgcttgg tcggaatagg tggtcgcgtg ccgaaccacg aattctatgg
      481 cctggttcga atcgagagcg gcacgagaaa tacgcacctg aaaatactcc agggactccg
      541 agcacaccca ggggtaatgt tgtttccacg cttcgaggcg ccgcgccata agggaaggcg
      601 caaacaattc cgtgagcgag gaggccacgg catcgagaag cggagcctct cggacgaacc
      661 ggacatagtc gtcgcatgcg agcctgactc ccgggagtac gtaccggaaa ctcctcgctt
      721 cctctgcgtc gagaccgacg cctcgtgcca actcaagcca tagggcgagt ccgccttctc
      781 cttcctgttc gccgtcatgg tcccggatgc gacggagcca catccgccga aacgtgggat
      841 cctcggattt cgaaacaatg agggcatcct tgatgggaat tcgcgtttga tagtagtagc
      901 ggttcaatac ccactgttgg agctgtgtct tcgtcaaatt tccgttgtgc atacgctcat
      961 ggaaaggatg gcggtcatga tagcgacgag agccttcccg cttgagccat tcgataaagg
     1021 catcctgtga gagccgatcc cgatggtgat cctccttcat catcacaaca ggatctccat
     1081 tccgtcccat gcgatctccc atcctgccgc ctcgacaagt tttcgttcgt gagaatcctc
     1141 tcgtaacatc ggattggtat tgttgatgtg gataaagacc cggcgagacg cggcaatctt
     1201 cgcaagcatt gcaaggcttc cctcgggtcc accgacaggg caatgtgcga gatcttccgc
     1261 cgattttgca aggaagcccg ggacggacag ttcatcgctc gaccaaaacg tcccgtcgaa
     1321 catgacgcaa ccggctcctt cgagcgcgtt gagaactgaa gacgtaatcc ggccgacggc
     1381 ggaaaaatag accagcactc ccccgttggt cgattggcga aatcgaagtc ccacattcac
     1441 gtccgtttca ctggatgaaa cgagtccttc caaatggatc ggaggcttgc ctggaacggc
     1501 cacggctgtc accgtcagtc ccgacggcct tccatccgca agcaacaccg gttcctcaac
     1561 atcgagtttc aacgtccgcc atgtgacctg tccggcgaaa cgctgcagtg tccgatacaa
     1621 gacgttgcct tcggtgaatc cgcgacgcac ggagtcggtt gcatacagga cgagccggtg
     1681 attctcccgg agcgagagga gccccaagca gtggtccaga tctccattgg tcaaaaagac
     1741 ggcttggatc ggtgtagaac gcgattgacg gggatggagg gagccgaagc tttcgatctg
     1801 agcacgaatg tccggagagg cgttgatgag aaaccagtta ccatcacccg tgctgaaact
     1861 gatggattcc tgggtccgag ggctcgcggc gatcagccct ttccgcatgc ctttgcaatt
     1921 cacacaggcg cagttccact gtgggaatcc acctcctgca gccgacccta gaacccgaat
     1981 gagcatcaca tccatccatt gctattcttg acgagtggtg ataaccggtg tctccgtcga
     2041 tgggtccttc gcgtcgggac cgtctcgaaa atcatcctgg tacgagttga tctcggcgtc
     2101 cattttgatt tccacaaatg aaggcctttc ccaattcatg atcgttcctc cttctattat
     2161 caggaatatg tcgatcatcg ccggaactcg gttcgcattt caatgttctc ttttccactc
     2221 ggcgggtgcg agccatgaac gcccctcgga aggccgcgat ggatagacag agagcgagga
     2281 gacccacggc ccgtctccgt gacagaccag gacgcggaaa tcatcgctgt tcagccgaac
     2341 ctccgaacga gaaagtttgc ttcgccttgt agcgggattt cggcggagtc aaaactggta
     2401 cctcccctag cctgcaccag tcacagcagg caggcgttat cttcgcactg tccggaaaca
     2461 ttgcacatta ccataaatac gatgtgccgg gataaattgt ccctgtagaa tacattttcc
     2521 agggttttcc cttgtgtcat cattaccatc ctccttgtta aggtttcggc atgcgtatat
     2581 ggccgggaca tccgttcccc ttaggcgccg attgggacgg tcagggcgta aatttcgctc
     2641 tattttcaga aaatgcaacc aaggtggaac tgtgtctgtt cgaccgcgcc gacgatccaa
     2701 aaccatccct gtgtcttccg ctcgaggagc agaccaatca agtatggcac acgtacctgc
     2761 cggaactgtt tcccgggcaa cattatgggt accgtgtcca tggcccctac gatccgaagc
     2821 aaggccatcg cttcaacccc gcgaagctgc tcatagaccc ttatgcgaaa gctattgccg
     2881 gcaatgtgca atggaccgaa ggacgcatgt tcggctatcg aatcggcgat ccagaagccg
     2941 atctgtcgat ggacgaccgg gataatgccg ccgatgtccc caaaagcgtc gtggtcgatt
     3001 ccgcgttcac ctggggtgcc gataaactgc cacgcacagc ctgggacgag acagtgatct
     3061 atgaggtgca tgtgaaggga ctcaccgccc gccatcccga agtgccggaa gcccagcgcg
     3121 gaacgtattt gggcatgaca tcacccgctg tcctggatca cttgaaaacc ctcggggtga
     3181 ccgccgtcga attgttgccc gtgcatcact tcgttcgcga tcaacatttg cacgaccgcg
     3241 ggctcacgaa ttattggggc tataattcaa tcgggttttt cgcgcccgac atccggtatg
     3301 cctattctca gaaacgcggc gagcaagtac gcgagttcaa gacgatggtg aagacgctgc
     3361 acagcgaggg catcgaagtc atacttgacg tggtctataa ccataccgcg gaaggcaacc
     3421 atctcggtcc gacgatctgc ttccgcggca tcgataacgc ggcctattat cgcctggtcg
     3481 acgacaacaa acgctactac atggactaca cgggaaccgg caacacactg aacatgaatc
     3541 atccgcgtgt gttgcagctg attatggaca gcttgcgcta ttgggttgaa gagatgcacg
     3601 tggacggatt tcgtttcgat ctcgcgtcca cgctcgcgag ggaactgcac gacgtgaacc
     3661 gcctgggtgc cttcttcgac atcatttatc aagatcccgt cctgtctcgc gcgaaactga
     3721 tcgctgaacc gtgggacttg gcttccggcg gctaccaggt cggtaacttt cccttgggct
     3781 gggccgaatg gaacgacaag taccgcgata ccatccgtcg ctatgtgaag ggcgacggag
     3841 gacaagtttc cgagttgggt taccggattt ccggcagcag tgatctgtac gaacgcagcg
     3901 gaaaacggcc gtacgccagc atcaacttcg tgaccgctca tgacggcttc acgctcgacg
     3961 acctggtttc atacaacgag aagcacaatg aagcgaacgg agaagagaat cgagacggta
     4021 acgacaataa ccaaagctgg aactgcggcg cggaaggacc gaccaacgac ccggcgatca
     4081 tggccctgcg ggaccggcaa aagcgcaatt ttttggcgat tctgctgctt tcccagggag
     4141 tgcccatgct ctgcggcggc gatgaactgg gccgcacgca gggaggcaac aataacgcct
     4201 attgtcagga caatgagatc agttggttcg attgggattt gaaagaccgt gatcgccaac
     4261 tgctggcctt cgtgcggcga ttggttcaat tacggcacga acaacccgta ttgcgccgac
     4321 gtcagttctt tcaaggacgg cgcatccgtg gatctgagat caaagacata gcctggttcc
     4381 gtccggacgg ccatgagatg accgatcaag actggcagga cgcccatgtc cggtccctgg
     4441 gtgtacggct ggccggtgat gccattcaag aaaaagacta taaaggccac tccgtatacg
     4501 gtgacacgct cctgttgctg tttaacgcca atcacgaacc tctttcgttt actctccccg
     4561 cgcatcagcg gggagtccgc tgggaaatac tcctcgatac cagcgaccct gaag
//
LOCUS       DATNDD010000002         3363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_571080, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000002 DATNDD010000000
VERSION     DATNDD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571080"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..2651
                     /gene="shc"
                     /locus_tag="VN647_00030"
     CDS             468..2651
                     /gene="shc"
                     /locus_tag="VN647_00030"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWG95440.1"
                     /translation="MNIIKALFNRLSDSLLATVQGRLDPATEFTPIAPLRLVSDKPIV
                     LPSLDSSAARRPIGHVVSQPDAVDDAIRRSQSWFFSRQHAEGYWVAELEADTTLTSEY
                     LMLRRFLDRVDPDREVKAVRYLKMMQLPDGGWPIYYGGPAEISASVKAYFALKLSGVS
                     ADEPCMVRAKERILAMGGVLQANVFTKITLALFDQYDWEGVPHMPVELMLLPKKFYFS
                     IYAISYWSRAVLIPLLIVFAHRPVCRIPCEQGIDELYPIPRAEVRYWKYPPFNKDQAW
                     FTPHNFFVALDAMLKLYDRMPMRMLREKALHKAACWVVDHVKGAGGLGAIYPAMANSI
                     MALECLGYGADDPLVVKALREIEELEVYDSTTIDGEIVPTLHLQPCFSPIWDTALLMN
                     ALVEAGVPQDHPVLQKAGRYLMSRQTKTVGDWIISSPNAEPGGWYFQFENELYPDVDD
                     SAVVIMALSKLKIPGREGELNESIRRGMRWVLAMQGSDGGWGAYDKDNNRIVFNYIPF
                     ADHKALLDPSTSDLAGRCLEMLGALGYDKTHPAAGPALAFLKKEQEEDGSWYGRWGIN
                     YIYGTWSVLAGLRAIGEDLSTPSIRRAVAWLESKQNSDGGWGESCLSYKDDPEHHGRG
                     ESTPSQTAWALMALMSAGAIDSFSVARGVQFLLRRQMKDGSWEEIAHTGTGFPRVFYL
                     RYHWYCQYFPLWALAMYRNLRSRGKMRADELRHHIQGTDSYRSEH"
     gene            2690..>3363
                     /locus_tag="VN647_00035"
     CDS             2690..>3363
                     /locus_tag="VN647_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95441.1"
                     /translation="MPPKRIAIFAATPWEMGAVRSAFPHGVERRIGRLPVFVRIVGNR
                     EYWLAQTGVGLEKAGRNAAHLLDNQSFSLIVSTGFACALNAADIGTLLVGREVVHQGA
                     RSDEPSEVLDVPGDERDRVMTWVTSLVPPEHIGRFVSTDRVIGSAREKRRFALSTQAI
                     GLDMESSALAAQAQRARVPFVIIRSVSDLLDEDLPLDFNLFLRPTGWLKGIGAVLAAP
                     SCLLGLG"
BASE COUNT          693 a          876 c          973 g          821 t
ORIGIN      
        1 gatcaccgag aaggttgtcc tgatcgccga acaggaggta agcgtcatgg tgatgacaag
       61 cagcaacaat gaaagcgtga gcctgttcat ccttaggtca acgcctctta tctgagtttc
      121 ggatgaagaa ccgacctgag acattcaaat accaggaaag tacggatgca gacggatgca
      181 ggtggttgat gaccgtggtt ctatcacacc caagatcgac atcggagatt gatgttgcat
      241 cgtgcagcta cgaatgctgt taacgtggtg ttcgcgatcg cctacaccga taaaaaatat
      301 agatacttca ggcacttctc agctccttcg cttgactcgc cttcaatcgt ttgttataag
      361 ccttccgtgt attagtaagg agtgttggaa aggtttacga agggagacca ttgtaagatg
      421 tcggtctttc gcctgccgca gttgtctctt ttgagtccta accattcatg aacatcataa
      481 aggcgttatt caatcgcctc tctgacagcc tcctcgcgac cgttcagggg cgactcgacc
      541 cagctacgga gtttacccct attgctccgc ttcggttggt ctcggacaaa ccgattgtcc
      601 tgccttcatt ggattcttcc gctgctcgcc ggcccatcgg ccatgtcgtc agccaacccg
      661 atgcagtgga tgacgcgatc aggcggagtc aatcgtggtt cttcagccgt cagcatgccg
      721 aagggtactg ggtcgccgag ctggaagcag ataccacgct gacctccgaa tacttgatgc
      781 tgcgtcgttt tctcgatcgt gtggatcctg atcgagaggt gaaggccgtc cgatacctca
      841 aaatgatgca actccccgac ggtggatggc cgatttatta tggtgggccg gcggagatca
      901 gcgcatcggt caaggcatat tttgcgctca aattaagcgg tgtgtcggcg gacgagccgt
      961 gtatggttcg agccaaagaa cggatcttgg cgatgggcgg tgtcctgcag gccaacgtgt
     1021 ttacgaaaat cacgctggcc ttgttcgatc agtacgattg ggaaggtgtt ccccacatgc
     1081 ccgtcgagct gatgttgttg ccgaagaagt tctacttcag tatttatgcg atatcgtatt
     1141 ggtcacgggc cgtgctcatt cccttgctca tcgtattcgc gcaccgtccg gtctgccgga
     1201 ttccatgcga acaaggtatc gacgagttgt acccgatccc gcgcgcggag gttcgctatt
     1261 ggaaataccc tccgttcaat aaggatcagg catggttcac tccgcataac ttcttcgtgg
     1321 cactggatgc gatgttgaaa ctgtatgacc ggatgcccat gcggatgttg cgggagaagg
     1381 cgctgcataa agcagcctgt tgggtggtcg atcatgtcaa aggggccggg ggactcggtg
     1441 ccatctatcc ggcgatggcc aactccatca tggcgcttga atgcttaggg tatggcgccg
     1501 atgacccgct ggtcgtcaag gcacttcgcg agatcgaaga attggaagtc tacgattcca
     1561 cgacgatcga cggtgagatc gtccccactc tccatctcca gccctgtttt tctcctattt
     1621 gggataccgc gttgctcatg aacgctctgg ttgaagcggg agtgccgcag gatcatcctg
     1681 tgcttcaaaa ggcgggccgg tatctcatgt ccaggcaaac caagaccgtg ggtgactgga
     1741 ttatctcctc gccgaacgca gaaccggggg ggtggtactt ccagtttgag aatgaattgt
     1801 acccggatgt cgacgattcc gctgtggtca tcatggcgct ctccaaattg aagattccgg
     1861 gtcgggaggg cgaactgaac gagtccattc gtcgcgggat gcgatgggtc ttggcaatgc
     1921 agggatccga cggcggctgg ggcgcctatg acaaggacaa caatcgaatc gtcttcaact
     1981 atatcccctt tgccgatcat aaagctctct tggaccccag tacctccgat ctggccgggc
     2041 gatgcctgga gatgcttggc gcattaggat atgacaagac ccatccagcc gccggaccgg
     2101 ccttggcttt tctgaagaaa gagcaggagg aagacggcag ttggtacgga cgatggggca
     2161 tcaattacat ctatgggact tggtcggttt tagccgggct cagggccatc ggcgaagatc
     2221 tctcgacacc ttctattcga cgggcggttg cttggctgga atcgaaacag aattctgacg
     2281 gtgggtgggg cgagtcctgt ctctcctata aggacgatcc tgagcatcac ggtagagggg
     2341 agagcacccc ctcacagaca gcatgggcct tgatggccct gatgtctgca ggagcgattg
     2401 attccttcag tgtcgcgcga ggagtgcaat tcctccttcg ccgtcaaatg aaggatggat
     2461 cgtgggaaga aattgctcat accggtacag gattcccgcg cgtgttttat cttcggtatc
     2521 attggtattg tcagtacttc ccgctgtggg ctctggcgat gtaccgtaac cttcgttccc
     2581 gtgggaagat gcgggccgac gaacttcgtc atcacattca aggaactgac tcgtatcggt
     2641 ccgagcattg acccctgccg gttctctttc cccttgtcca tctggtactg tgccgcctaa
     2701 acgaatcgct atttttgccg ccactccatg ggagatgggt gcagtgcgat cggcgttccc
     2761 tcacggcgtt gaacgtcgaa tcggtcgtct cccggttttc gtgcgtatcg taggcaacag
     2821 ggaatattgg cttgctcaaa caggcgtcgg tcttgaaaaa gcggggagaa acgccgccca
     2881 cctgctcgac aaccagtcat tcagcctcat cgtgtcgacg gggtttgcct gtgcgctcaa
     2941 cgcggcggat atcgggacac tcttggtagg ccgtgaagtg gtgcatcaag gagcgcgcag
     3001 cgacgagccg tcggaggttc tcgatgtacc gggtgatgag cgggatcgcg tcatgacgtg
     3061 ggtcacgagt ctggtgcccc ctgagcacat cgggcggttt gtctcgaccg atcgagtcat
     3121 cggcagtgcc cgggaaaagc gaaggtttgc gctgagcact caggccatcg gcctcgatat
     3181 ggaaagttca gctttggctg cacaagcgca acgggcacgg gtcccttttg tgatcatccg
     3241 atctgtttcg gatcttttgg acgaagatct tccgcttgac ttcaatctgt ttctcaggcc
     3301 cactggatgg ctcaaaggta tcggtgccgt tttggccgct ccttcatgtc tcttgggtct
     3361 tgg
//
LOCUS       DATNDD010000003         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1435963, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000003 DATNDD010000000
VERSION     DATNDD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435963"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /gene="aroF"
                     /locus_tag="VN647_00040"
     CDS             complement(<1..789)
                     /gene="aroF"
                     /locus_tag="VN647_00040"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWG95442.1"
                     /translation="MIIVLKPEASESEVDHIIDRLRDLGLKSQISTGQERTIIGVIGD
                     DRILHNQPLTALPGVESVLPILAPWKLVSREFKKEATIIDVGGAKIGAKKLAIMAGPC
                     AVERLELTVGIAHEVKAAGASVLRGGAYKPRTSPYSFQGLGREGLDYLLEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNQNVMLCERGIRTFETQYRNTLDLEAIPTLKELSH"
     gene            complement(786..1892)
                     /gene="hisC"
                     /locus_tag="VN647_00045"
     CDS             complement(786..1892)
                     /gene="hisC"
                     /locus_tag="VN647_00045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWG95443.1"
                     /translation="MALQVHPDILSLSPYIPGKPIDELQRELGLSRVIKLASNENPLG
                     PSPKALTALSGAQDMLHRYPDGSAYRLRQAIADRWKVTEGQVILGNGSDEILGLLAKT
                     FLTPGDEAIMADHTFVIYKMEVTAVHGKPVVVPLVNWTHDLESMVRAVTPRTRLLFLC
                     NPNNPTGTIVLAEAVDRLMVTVPEDVIVVFDEAYFEYVRNPRFPDAMAYVKQRRNAIV
                     LRTFSKIYGLAGLRIGYGISTEEIIDYLNRVRPPFNANSFAQKAALAALGDDEHVAKS
                     RTVNAAGMEQIERGLRALGMTSIPSETNFLYFDAKRNGGLVFEALLREGIIVRHIDGT
                     MIRVTIGQPDENAAFLQALEKVLHGERKEIAREL"
     gene            complement(1895..>2500)
                     /locus_tag="VN647_00050"
     CDS             complement(1895..>2500)
                     /locus_tag="VN647_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HWG95444.1"
                     /translation="DSNLLIYGEILQEVSHHLLSKSGLIEDVKKVYSHPHALAQCRNW
                     LETNLPHVPAVEVASTARAAELCIDEPPSAAIASELAGQLYGLKVIKARIEDNLNNFT
                     RFLILSQKPSERTGKDKTSLMLSVKDKVGALYDLLRPFASHGINMTKIESRPSQRKAW
                     EYIFFVDVEGHIHEERVNRAVEEVKGRCLFMKILGSYPIHS"
BASE COUNT          550 a          691 c          632 g          627 t
ORIGIN      
        1 atgcgagagc tctttcaacg tgggaatggc ctcaagatcc agcgtattgc gatattgtgt
       61 ttcgaacgtg cgaatgcccc gttcgcacag catgacgttc tgatttccgc gtgacatgat
      121 gtattcggcc gacaggagaa actctttgat cgtcgccgac aggcctcgtt tcaggagcac
      181 cggtttgtcg taggcaccga cctctttcag gagctcgaag ttctgcatgt tccgagcgcc
      241 gatctggatg atgtccgcct tctcgagaaa gagttcgatg tctctggtgt ccaagatctc
      301 actgacgacc ggtagcccgg tttgcttttt tgcttcaagc aagtagtcaa gcccttctcg
      361 acccaaccct tgaaaggaat acggcgatgt tcttggttta taggctccgc cacgaagtac
      421 cgaggctccg gcggccttca cctcatgtgc gatccctacc gtgagctcaa gccgttcgac
      481 cgcacagggg ccggccatga tggccaactt cttggcaccg atttttgcgc caccgacgtc
      541 gatgatggtc gcttccttct taaactcacg actgaccagt ttccatggcg caaggatcgg
      601 cagaacgctc tccactccag gaagcgcggt cagaggctga ttgtgcagga tccggtcgtc
      661 tccgatgacg ccgataatcg tgcgctcctg accggttgat atttgcgatt tcaaacctag
      721 atctctcagt cggtctataa tgtggtccac ttcactttcc gacgcttctg gcttcaacac
      781 gatgatcata attccctcgc tatttctttc ctttctccat gcaagacttt ctcaagtgct
      841 tggaggaagg ccgcattttc gtcgggctga ccgatggtga cacgaatcat tgtgccatcg
      901 atatgccgca caataattcc ctctcgcagc aatgcttcga acaccagtcc cccattccgt
      961 ttcgcatcaa aatagagaaa attcgtctcg ctcgggatgg aagtcatgcc cagcgcgcgc
     1021 agcccccgtt ctatctgttc cattccagcc gcattcacgg tccgactctt ggccacatgt
     1081 tcgtcatctc ccaatgcggc caatgcggct ttctgggcaa agctgttggc attgaacgga
     1141 ggccgaactc tattcaagta gtcgatgatc tcctctgtgc tgatgccgta gccgattcgc
     1201 agtcccgcga gcccatagat tttggagaat gtcctcaaga caatcgcgtt ccgcctctgt
     1261 ttcacgtagg ccatcgcatc ggggaaccga ggattgcgga catactcaaa gtacgcttca
     1321 tcaaacacga cgatgacatc ttctggcact gtgaccatga gccgatcgac cgcttccgcc
     1381 aatacgatcg tccctgtcgg attatttggg ttgcagagaa agagcaatcg tgttcgaggt
     1441 gtaacggcac gcaccattga ctcaaggtca tgtgtccaat tgacgagcgg tacgaccacc
     1501 ggcttgccgt ggacggcagt gacctccatc ttatagatga cgaacgtgtg atcagccatg
     1561 atggcttcat caccgggagt caagaacgtc ttggccaaca agcctagaat ctcatctgat
     1621 ccatttccga gaatgacctg tccctcggtc actttccaac gatcagcgat cgcctgcctg
     1681 agccgatacg cacttccgtc cggatatcga tgcagcatat cctgtgcgcc gctcagtgct
     1741 gtcaacgctt tcggtgaagg cccaagcgga ttttcgttgg aggcaagctt tatgacacgg
     1801 gagaggccga gctctcgctg tagctcgtcg atcggtttac cggggatgta tggactgagt
     1861 gacagaatat ctgggtgaac ctgtagtgcc atggtcaact atgaattggg taggagccca
     1921 aaatcttcat gaagaggcag cggcccttta cttcttccac tgctctattg acccgctcct
     1981 catggatatg gccttcgaca tccacgaaaa aaatgtattc ccacgccttt cgctgggaag
     2041 ggcgagattc gatcttggtc atgttgatcc cgtgagaggc aaagggacga agcagatcat
     2101 acagtgcgcc gactttatcc ttcaccgaca acattagcga tgttttatct ttccctgtgc
     2161 gctccgacgg cttctgcgac agaatcagga aacgcgtaaa attgttgaga ttatcttcga
     2221 tacgggcctt aattactttg agcccgtaca gttgcccggc caattccgac gcaatcgcag
     2281 ccgaaggtgg ttcatcgatg cacagttcgg cggcacgagc agtgctggct acctcaactg
     2341 cggggacatg cggaagattg gtctctagcc agttccggca ttgcgcaaga gcatgaggat
     2401 gggagtaaac tttcttcacg tcctcgataa gtcccgattt tgacaagaga tgatgtgaga
     2461 cctcttgaag aatctctcca tagatcagca agttggaatc
//
LOCUS       DATNDD010000004         9426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1427160, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000004 DATNDD010000000
VERSION     DATNDD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427160"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VN647_00055"
     CDS             <1..301
                     /locus_tag="VN647_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HWG95445.1"
                     /translation="GGSAGAEHSPPGRSRHGGRTVEMARVYVGVGREAGIRPGDLVGA
                     IANEARLNSNIIGAIEIEQRFSIVAVPASMASGIIEVLGRARIKGRRVPVRLFRD"
     gene            330..2369
                     /locus_tag="VN647_00060"
     CDS             330..2369
                     /locus_tag="VN647_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWG95446.1"
                     /translation="MKNSQPLVPATVRLPEITVKAVLLSVILAAVLAAANAYLGLFAG
                     MTVSASIPAAVASMAILRLFRESNVLENNLVQTAASSGEALAAGVIFTIPALLLVGYW
                     SDFDYWQTVLIATVGGLLGVLFTIPLRRALIVTAALRFPEGLATAEVLKVGAAERSEA
                     GSRTSTKNFQSLLSAAFLGGLVKFGESGLRLWTDSLEGATHVGRTVFYAGLNLSPALL
                     AVGFIIGLQTAAVVFLGGVIGWVILMPAYGLVQGLPPDRTGPAAAMSIWSGQIRYVGI
                     GAMLTGGLWTLTKLREPVWKSLQTLRASYRGVQVKGTAQVEIKEHLKRENVPRTEQDA
                     SFLWIVVPFGLSLLPMAWIYSTVVGSPLLGLLMTIVMALAAFLFSSVAAYMAGMVGSS
                     SNPVSGVTIATIMIAALLLVLFMGAGHPAGPAAALVIGAVVCCAAAMGGDNLQDLKTG
                     QLVGATPWKQQVMQVVGVVTGALVLVPVLSLLQAKYGIGEPTSSHPHPLSAPQAMLMA
                     NLTRSVFGAGLPWHLVGLGAVIGILIILADRRQERRGSALRLPVLAVALGIYLPLKLS
                     ATIFVGGLIAALANRRLGGRQESSQGGLFFAAGLITGEVLMGIMLAMPIAFAALWPGF
                     STDPFMIFAVPPFGGWPGLVMVVVVGFALYRIAVERGQNALTSTTEAASQDTSSE"
     gene            2482..2787
                     /locus_tag="VN647_00065"
     CDS             2482..2787
                     /locus_tag="VN647_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HWG95447.1"
                     /translation="MKISTRFLELLRMIGRLWTDLPLLFRLLKAWKDGSYRSLSVRTL
                     AVLVAALIYVLSPVDLMPDFIPGIGLIDDAAVLAFLLHSMAQDLAVFRAWEQSRRSV"
     gene            complement(2832..3014)
                     /locus_tag="VN647_00070"
     CDS             complement(2832..3014)
                     /locus_tag="VN647_00070"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HWG95448.1"
                     /translation="MARYGKRAQKSVERAMHKRKKGTLKSGKSGKPVKSRKQAVAIGL
                     SEARRKGAKVPKKKAA"
     gene            complement(3074..3385)
                     /locus_tag="VN647_00075"
     CDS             complement(3074..3385)
                     /locus_tag="VN647_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95449.1"
                     /translation="MKAAILPVLVAGFLLAGVGAAYSGNPMSEKEASPTLKERLTKDT
                     IKGTLMRTECEYYYIKDNDGKEQKVHVDKSTKLDKVLPGDMVKAYVTDKGHTTTLERV
                     N"
     gene            complement(3557..3904)
                     /locus_tag="VN647_00080"
     CDS             complement(3557..3904)
                     /locus_tag="VN647_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95450.1"
                     /translation="MSFLFAPLAGADLLEMADLMAHPEVYDHKTVVVIGLVKDVQLVI
                     DQKGEPTFKFLLADDDGMIKVTSRIQVRNGDQVIVEGMFTRRRQGGRLTVYSEVNAFT
                     IRPLNQFHSDLVG"
     gene            complement(4114..4338)
                     /locus_tag="VN647_00085"
     CDS             complement(4114..4338)
                     /locus_tag="VN647_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95451.1"
                     /translation="MPSHHRLEKKLPHEVDITGIAVALAFLVIVGVFAGGRGNRTVSE
                     DGQPPPVSMQVAPTLTIEGDRGSTAAGRQT"
     gene            complement(4414..6939)
                     /gene="hrpB"
                     /locus_tag="VN647_00090"
     CDS             complement(4414..6939)
                     /gene="hrpB"
                     /locus_tag="VN647_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HWG95452.1"
                     /translation="MSTLPIEDILPSVCRALEAGPNALLTAPPGAGKTTRVPLALLNA
                     SWLSDRKLLLLEPRRLAARAAAHRMAEVLQEHVGETVGYRMRLETKIGPTTKIEVVTE
                     GVLTRLLQQDPSLEAYGIVLFDEFHERSLQADMGLALCLETQRLFRPDLRLFIMSATL
                     DCGPVAALLGRAPLITCEGRMFPVETRYLDQPLTGRLDAAVSQLIRRSLAKDHGSLLV
                     FLPGMAEIRRVERLMMDSNLDHSVQIAPLHGDLPQDMQDVAIRPTAPGRRKVVLATSI
                     AETSLTIDGIRGVIDAGLVRIPRYDPRSGLTRLETIRVTKDSAEQRRGRAGRLETGIC
                     YRLWTEKEQAGLAPRRPPEILEADLTPLMLDLAQWGTRDPSELSWLTPPPAGAVAQAR
                     DVLVQLGAFSADGRPTAHARRMVELPLHPRLAHMMLRAVSLGLADLACKVAALLSERD
                     ILHGPRERQNADLRVRLDILGEQYDSIGLEVNRATVDRVRRTAGLWRRRLISEQSREA
                     EGNGYDRSNSIGLLAALAYPDRIARRQSDCEGRYRLVNGRGARFKTADPLAIEPFLVI
                     ADLDGRDQWSEINLAVPIAQKDIVSLYHDRLVDEKEVIWDDALRAVRAVRRRRLGSMI
                     LVEEAVSSPDVNEVKSALLQGIHKCGLEVLSFGHLLQQWRARVMWLRRIDDPRSEWPD
                     LSDEALLQTLDQWLGPYLTGITTLDRVKRLDLTTPLHALLTHEQLRRLDRLAPTHITV
                     PSGSRLPLDYEQPESPVLAVRLQEMFGCKDTPRIAGGKIPVILHLLSPAKRAVQVTQD
                     LDGFWQRSYQDVRKELRGRYPKHHWPEDPIGAAPTAKAKQRNR"
     gene            7000..7230
                     /locus_tag="VN647_00095"
     CDS             7000..7230
                     /locus_tag="VN647_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95453.1"
                     /translation="MKNRSAREEEPMSPYRWSHNKPTKPGWYWFRGPAHEADPFIVLV
                     DQAGEFQWPDGGFQEVSLANGEWAGPIEEPKE"
     gene            7333..8796
                     /gene="zwf"
                     /locus_tag="VN647_00100"
     CDS             7333..8796
                     /gene="zwf"
                     /locus_tag="VN647_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWG95454.1"
                     /translation="MMKETVEPHLFIILGATGDLTRRKLLPALFHLRTYGEQEKQNTL
                     IVGAALPEMSADAFRLWAYEGLRNSGARDASGLRRWCEDQLYYQPLREGKLSDYQALA
                     AFIARLEVARNLPQNRIFYLALPPTIVPETLELLDQAGLLKSRGWVRAVLEKPFGHDF
                     HSARALNTLVHRYLDESQIYRIDHYLGKETVQNLLAFRFANPIFESLWNRNTVENVEI
                     TVAEDLGVEHRGAYYQKAGAFRDMVQNHLTQLLTVVGMEVPTSFEASEIRAEKLKVLR
                     SISSIRPQDVIFGQYTAWDVAGQRIPGYLEEPGVSPDSTTETYVALKLEIHNWRWRGV
                     PFYLRTGKRLPRKITQVAVTFRAAPTQVFRSLEPGSLNPNKLLITLQPSEGFSLCFSV
                     KTPGRPFKFTDRALRFDYSQGFGELPEAYETLLRDVMIGDQTLFVTADFTETAWRLYD
                     PLLLDPRPVHLYTAGSWGPKEADVLVERNGLNWQLGW"
     gene            8809..>9426
                     /locus_tag="VN647_00105"
     CDS             8809..>9426
                     /locus_tag="VN647_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG95455.1"
                     /translation="MDRRRPIAVLVGRMPPDMPITTNIPQRMDRLPWARWHWLVVGAL
                     GITWLLDGLEVSIVASLGPTLTHPDTLHLTQSEVGLTASVYLAGSVLGAFLFSYLTDR
                     QGRKKWFMITLALYLTATVLTAFSWNLMSFLFFRFLTGAGIGGEYAAINSAIDELIPA
                     RNRGHTDLAINGTWWLGSAAGALMTLLLLNPAIFTESIGWRLCFVL"
BASE COUNT         1946 a         2607 c         2702 g         2171 t
ORIGIN      
        1 cggtggatcg gctggagcag aacattcgcc gccaggacgg tcgcggcatg gcgggcgaac
       61 ggtcgagatg gcacgcgtgt atgtcggggt ggggcgtgag gcaggcatca gacccggcga
      121 tttggtgggc gcgattgcga atgaagcgag gcttaactcc aacatcatcg gagccatcga
      181 aatcgaacag cggttttcca tcgttgctgt accggcctcc atggcctcgg gcatcatcga
      241 agtactcggc cgcgctcgca tcaagggccg cagagtgccc gtccgattgt ttcgcgacta
      301 gcttgtgtga tcacgcaagg ctggctatca tgaaaaattc ccagcctctc gtccctgcca
      361 cggtccgact tcctgagatt accgtcaaag cggtcctgtt gtcggtcatc ctggcagcgg
      421 tcttggcggc tgccaatgcc tacctcggct tgtttgccgg catgacggta tccgcgtcta
      481 ttcctgcggc agtcgcttcc atggcgattt tgcggttgtt tcgtgagtcg aatgttctcg
      541 aaaacaacct cgttcaaacc gccgcttcat cgggcgaagc attggcggca ggcgtcatct
      601 tcacgattcc tgcactccta ctggtcgggt attggtccga cttcgactac tggcagacgg
      661 ttcttattgc cacagtcggc ggattactcg gtgtgctgtt caccatcccc ctgcgacggg
      721 cgttgatcgt aacggcggct ttgcggttcc cagagggatt agccacggcg gaagttctca
      781 aggtcggcgc tgcggaacgg agtgaagccg gttcacgcac atcaacaaag aattttcagt
      841 ccctgttgtc ggctgctttt ctcggtggct tggtgaagtt cggggagagc ggcctgcgcc
      901 tgtggaccga ctcgctggaa ggggctactc acgttggacg caccgtgttc tacgccgggc
      961 tcaatctttc gccggcgctt cttgcagtcg gattcattat cggtctacag acagccgcgg
     1021 tggtctttct tggaggggtc atcggatggg tgatcctgat gcctgcatat ggacttgtcc
     1081 aagggctccc acctgatcgg acaggaccgg cggcggcgat gtcgatctgg agcgggcaga
     1141 ttcgatacgt gggcatcgga gcgatgctga ccggcgggct gtggacactc acgaaattgc
     1201 gtgagccggt ctggaagagt cttcagacgc tccgggcaag ttatcgggga gtccaggtta
     1261 agggtacggc tcaggttgag attaaggaac atctgaagcg cgaaaacgta cccaggacgg
     1321 aacaggatgc ttcattcctg tggatcgtcg tgccatttgg cctgtcgctc ctgccgatgg
     1381 cctggatcta ttcgacggtc gtgggcagtc cccttctcgg tctgctgatg accatcgtga
     1441 tggcacttgc cgccttcctc ttctcgtcgg ttgccgcgta tatggcgggg atggtcggaa
     1501 gttcaagcaa ccccgtctca ggcgtgacca tcgccactat tatgattgct gcactgctgc
     1561 tcgtactttt catgggagcc ggacatccgg cgggaccggc ggctgcattg gtcatcggag
     1621 cagtcgtctg ttgcgccgcc gcgatgggcg gagacaatct ccaagatctc aaaacaggtc
     1681 agctggttgg ggcgacgcca tggaaacaac aagtcatgca agtcgtcggg gtggtgacgg
     1741 gcgctctggt cctcgtgccg gttctgtctc tcctccaagc caagtatgga atcggtgagc
     1801 cgacgagcag ccatccacat cctctcagtg cgccacaggc catgctgatg gcgaacttga
     1861 cacgaagcgt gttcggagcc ggattgccct ggcatctcgt aggattgggt gctgtgatcg
     1921 ggattctgat cattctggca gatcggcgtc aagaacgccg tggcagcgcg ttgaggttac
     1981 ccgtgcttgc cgtggcgctt gggatctact tgccgctgaa actttcggcg accatttttg
     2041 tcggtggact cattgcggct ttggcgaata ggcgtttggg tggaaggcag gagtcgtcgc
     2101 agggaggatt gttctttgcc gccggactga tcaccggcga agtcttgatg ggaatcatgc
     2161 tcgcgatgcc gatcgcattc gccgcccttt ggcccggctt cagcaccgat ccatttatga
     2221 tttttgccgt tcctccgttc ggggggtggc ccggccttgt catggtggtc gtcgtgggat
     2281 tcgcgctcta tcggatcgcg gtggagcggg gccagaacgc gttgacatcc acgactgaag
     2341 cggcgtcgca ggatacctca tcggagtgag atatcacgat gagtatcacg gcaagaccgg
     2401 acacgacttc ctaaaatcgt cgtcccttgg tattctaaat gccgctgctg gttcgtaaca
     2461 caagtcatca cggaggtgaa catgaagata tcgacgcggt ttttggagtt gttgcgcatg
     2521 atcggtcgtc tctggacgga tcttcctctc ttgtttcgac tgctgaaggc ctggaaagac
     2581 ggcagctatc ggagcctgtc cgtgcgcaca cttgccgtgc tcgtcgccgc actgatctat
     2641 gtattgagtc cggtcgatct catgccggac tttattcccg gcatcggctt aatcgatgac
     2701 gcagcggtac tcgctttctt gctgcacagc atggcgcagg atttagcggt atttcgagcc
     2761 tgggagcaga gccgccgctc tgtatgacgg cctcacacca tcacgaatcc tgaggaaaca
     2821 tcgtgcgctg gttatgccgc tttcttttta ggaaccttgg cccctttgcg tctcgcctcg
     2881 gaaagaccga tagcgacggc ttgcttgcgg ctcttcacag gcttcccact ttttccgctt
     2941 ttgagagttc ccttctttcg cttgtgcatc gcccgttcga ccgacttctg agcacgtttt
     3001 ccgtatcgcg ccatactgtc ctcctgatct atttcatcac ttgctcgttc ggtcaaaaac
     3061 aaagtcaatc gactcagttc acgcgctcta aggttgtcgt atgaccctta tccgtgacgt
     3121 aagccttgac catgtcgccc ggcagcactt tatccaactt agtgctcttg tcgacatgga
     3181 ccttttgctc cttaccatcg ttgtccttta tgtagtagta ctcacattct gttctcatca
     3241 aagtgccctt aatcgtatct ttcgtcaaac gctctttgag cgtcggcgaa gcttcttttt
     3301 cgctcatcgg attgcccgaa tacgccgcac caaccccggc taataagaat cccgcaacaa
     3361 gtaccggcaa tattgctgct ttcataaaga cctcctcttc ttattctcaa tatacctatt
     3421 cttgcgcgag acaactgaat tgagagctag gaaagtacct tggacattcg tcgatgggga
     3481 atagaaatga tttgaaagcg aactcgacgg aaaaatgatc gcgcgcaaaa aaggagataa
     3541 gaccgtggag gggagtttat cctaccaagt cggagtggaa ttgattgagc ggccgtatgg
     3601 tgaatgcatt cacttcgctg tacacggtga ggcggccgcc ttgtcgacgc cgggtaaaca
     3661 taccttcgac aatgacttga tccccattcc gaacttggat tcgactggtc actttgatca
     3721 tgccgtcgtc gtcggccagt aaaaacttga acgtcggttc gcctttctga tcgatgacca
     3781 gctgcacgtc cttcaccaag ccgatgacga ccacagtctt atgatcatac acttcgggat
     3841 gggccatgag atcggccatc tcaagtaaat cggcgccggc aagcggtgcg aacagaaagc
     3901 tcatgaagac agccagtggc gcccagaatc tgtaccgcct atgaaccagg gttttgcttg
     3961 ccatgatcgg ggcgtattgt accgaagatc atggcaagta caagatgggc aatcacctta
     4021 ctcaaaggtc tccagttctg ttacggggtg acggagtgat gggctgaccg tgattcagcg
     4081 ggggccctga gcgtgagcga ggtatcggag agatcaggtt tgtcttccgg ctgctgtaga
     4141 gcctcggtct ccctctatgg tcaaggtcgg tgcgacttgc atggaaaccg gcggaggttg
     4201 cccatcctcg ctcacagtgc gattgccccg gcctccggca aacacaccga cgatcacgag
     4261 gaatgcgagc gctacggcaa tccccgttat atctacttcg tgaggaagct ttttctctaa
     4321 tcgatgatgc gacggcatga gttccgtttt tcctgagcat gaggttgtgt actggatact
     4381 ctccccacac cgaataagcc acaaggtaga accctagcga tttctctgct ttgccttggc
     4441 ggtcggcgcg gcacctatcg gatcttcggg ccaatggtgc ttgggatatc gtccgcgtaa
     4501 ctctttacgg acatcttgat aggacctctg ccaaaagcca tcaaggtctt gggtaacctg
     4561 caccgcccgt ttggcagggg acaggagatg caagatcacg ggaatctttc cgcccgctat
     4621 acgcggcgtg tccttgcaac caaacatctc ttgcaagcgt accgccagca ccggagactc
     4681 aggttgctcg taatcgagag gaagtcggga accactcgga acagtgatgt gagtcggggc
     4741 gaggcggtca agacgacgca gttgttcgtg cgtcagtaag gcatggagcg gtgtcgtcag
     4801 atccagccgt ttcacccgat cgagggtggt gatcccggtc aagtaaggac caagccactg
     4861 atccaatgtc tgaagcaatg cctcgtcgga cagatccggc cattctgatc gaggatcatc
     4921 aatgcgtcta agccacatga cgcgagcacg ccactgttgg agcagatggc caaacgaaag
     4981 aacctcaagt ccacatttat gaataccttg taaaagtgct gattttactt catttacatc
     5041 tggtgatgag accgcctcct ctacaagaat catcgatcca agtcgtcggc gccgtacggc
     5101 tcgaaccgct ctaagggcgt catcccagat cacttctttt tcatcaacca accggtcatg
     5161 gtaaagggat acaatatctt tttgagcgat cggaacagcg agattgatct cggaccactg
     5221 gtcgcgtcca tccaagtcgg cgatcacaag gaagggttct atggccaagg gatcggcggt
     5281 cttaaaccga gcgcctcggc cattcacaag acggtaccgc ccttcacaat ccgactgccg
     5341 ccgagcaatt cgatcaggat aggcgagcgc agctaacagc ccgattgagt ttgaacgatc
     5401 atatccattg ccttccgcct cgcgagattg ttcactgatg agccgtctcc gccataggcc
     5461 ggccgttcgc ctcactcggt caactgtggc tcgattcacc tccagaccga tggagtcata
     5521 ctgttccccc agaatgtcca gcctgacgcg caaatcggcg ttctgccgtt cgcgtggccc
     5581 atgaagaatg tcccgttcgc tcaataacgc cgccaccttg caagcaagat cggcaaggcc
     5641 caatgacacc gcccgaagca tcatatgggc taggcgggga tgcaggggga gttcgaccat
     5701 tcgtcgggca tgggccgtcg gccggccgtc agccgagaag gccccgagtt ggactaatac
     5761 atctctggcc tgtgcgactg caccggcagg tggcggagtg agccacgaca gttccgacgg
     5821 atcacgtgtg ccccactggg caagatccag catgagagga gtgagatccg cctcgagaat
     5881 ttccggtgga cgacggggag caagcccagc ctgttctttt tcagtccaca gccggtaaca
     5941 aatacctgtc tcaagtcgtc cggcccggcc gcggcgttgt tcagcagaat cctttgtaac
     6001 acgaatggtc tccaatcgag tcaggccaga gcgaggatcg taccgtggaa tacgtaccag
     6061 gcctgcatcg atcaccccac ggatgccgtc gatcgtcaga ctggtttcgg cgatggaagt
     6121 ggcaagcacg acttttcttc gcccgggagc cgtcggtcga atcgcgacat cctgcatgtc
     6181 ctggggaaga tcgccgtgca gcggcgcgat ctgtacggaa tggtctaggt tggagtccat
     6241 cattagccgt tcgacccgac gaatttcggc cattcccggc agaaagacca acagactccc
     6301 gtgatccttt gcgagtgatc gtcggatgag ctgcgatacg gccgcgtcga gccgtccggt
     6361 aagcggctga tcaaggtacc gggtctcgac tggaaacatc cggccttcgc acgtgatgag
     6421 gggagctcgc cccaacagtg cggcaaccgg accgcagtcc aaagtggcgg acatgatgaa
     6481 gagacgaagg tctgggcgga agaggcgttg agtttcgagg cacagggcaa gtcccatgtc
     6541 cgcttgcaga cttcgctcgt ggaactcatc aaacaacacg atgccatagg cctccagcga
     6601 cgggtcttgt tggagcagcc tcgtcagcac tccctctgtc accacctcga ttttagtcgt
     6661 cggtccgatc ttcgtttcga ggcgcatgcg atagccgacg gtttccccga catgctcttg
     6721 taatacttcc gccatgcgat gagcggcggc tcgcgcagcg agccgccggg gttcgagcag
     6781 caagagtttt ctatccgaca accaggatgc gttcaggagc gccaagggga cgcgagtggt
     6841 tttcccggct cccggagggg cggtcagtaa tgcattcgga ccggcttcca atgctcggca
     6901 gactgacggc agtatgtctt ctattggaag tgtggacatg gcgtgatagg gtcatgaata
     6961 gagattgccc actctaccag aacatggaga caaggaggaa tgaagaaccg ttcagcacgt
     7021 gaagaagaac cgatgtcgcc ctatcgttgg agccacaaca aaccgacaaa gcctggctgg
     7081 tactggttcc gcgggccggc ccacgaagcc gacccgttta tcgtgcttgt cgatcaagcg
     7141 ggagagttcc aatggccgga cggcggattt caagaggtat cgctcgccaa cggcgaatgg
     7201 gccggtccga ttgaggaacc gaaagagtag atcgatgcct tacgggaaag gggacggagg
     7261 attagggggg cctccattga cgactgacaa aatgagtcat aacttcagta tatcttagga
     7321 atcgtcacgg ggatgatgaa ggaaacggtc gaaccacatc tgtttattat tctgggggcg
     7381 acaggagatt taacgcgacg gaaactgctg cccgcattgt ttcacttgcg cacctatggt
     7441 gagcaggaga agcagaatac gctcatcgtc ggagccgcat tgcctgaaat gagcgcggac
     7501 gcgttccgct tgtgggctta cgaggggctg cgcaattcgg gtgcccgtga cgcttctggt
     7561 ctccgtcgat ggtgcgaaga ccagctgtac tatcagcccc tgcgggaagg caaattgtcg
     7621 gactaccaag ccctcgccgc gtttatcgcc cgccttgaag ttgccaggaa tctgcctcaa
     7681 aatcgcattt tctatctggc gcttccgccg acgattgtgc ctgaaacctt ggaattactc
     7741 gatcaagccg gcttgctgaa aagccgcggg tgggtgcgcg ccgtcttgga aaaacccttt
     7801 ggtcacgatt ttcattctgc ccgtgcgctc aatacccttg tccatcgtta tctggacgaa
     7861 tcgcagattt atcgcatcga tcactatctc ggcaaagaga ccgtgcaaaa cttactggcc
     7921 tttcgcttcg cgaaccccat ttttgagtcg ctgtggaatc gcaatacggt ggagaacgtg
     7981 gaaatcaccg tcgctgaaga cctcggtgtt gaacatcgtg gagcctacta tcaaaaagcc
     8041 ggcgccttcc gtgacatggt gcagaaccat ttaacgcaac tgttgaccgt cgtcggtatg
     8101 gaggtgccga cttccttcga agcttccgag atccgggctg aaaaactgaa agtacttcgc
     8161 tctatttcat cgattcgtcc acaggacgtg atcttcggcc aatatacggc gtgggatgtc
     8221 gccggccaga ggattccggg ctacctggaa gaaccaggcg tatctccgga ctcgaccacc
     8281 gagacctatg tggcgctgaa gttggagatt cacaattggc gctggagggg agtgccgttt
     8341 tatttaagaa ccggaaaacg ccttcctcgc aagatcacgc aagtggccgt cacctttcga
     8401 gcggcgccca ctcaagtctt tcgatctttg gagccgggta gtctgaaccc caacaagctc
     8461 ttgatcacct tgcaaccgag tgaaggcttc tcgctttgct tttccgtgaa aactcccggg
     8521 cggccgttca agttcaccga tcgagcactg agattcgatt atagtcaggg attcggcgaa
     8581 ttgccggaag cctatgagac gctgttgcgc gacgtcatga tcggtgacca gacattattc
     8641 gtcaccgcag attttacgga aacggcctgg cgactctacg acccgctgtt gctcgatccc
     8701 cgaccggtgc atctctacac ggccggctcg tggggcccaa aggaagcgga cgtgctcgtg
     8761 gaacggaacg ggctgaactg gcaattgggt tggtaggcgc tccaagtatt ggacagacgg
     8821 cgaccgattg ccgtattagt agggcgcatg cctcccgata tgccgatcac taccaatatt
     8881 ccccaacgga tggaccggtt gccatgggca cgctggcact ggttggtcgt aggggcattg
     8941 ggcatcacgt ggctgctcga cgggcttgaa gtatcgatcg tggcctcact cggcccgacg
     9001 ttgacccacc cggacacatt acatctcacg caatcggaag tagggctcac ggcgtccgtc
     9061 tatctggcag gttcggtcct cggcgcgttc ctcttctcct atctcaccga tcggcaggga
     9121 cgaaagaaat ggttcatgat caccttggcg ctgtatctca cggcaacggt cctcacggca
     9181 ttttcatgga acctcatgag cttcctgttc ttccgatttc tgaccggagc cggcatcggg
     9241 ggcgagtacg cggccatcaa ttccgcgatc gatgaattga ttcccgcgcg caatcgcggc
     9301 cataccgatc tggcgatcaa cggaacctgg tggcttggat cggcggccgg cgctttgatg
     9361 acactgctct tgttgaatcc tgcgatcttc accgaatcca tcggatggcg gctctgtttt
     9421 gtcctc
//
LOCUS       DATNDD010000005         2934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_859042, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000005 DATNDD010000000
VERSION     DATNDD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859042"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VN647_00110"
     CDS             <1..897
                     /locus_tag="VN647_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HWG95456.1"
                     /translation="ALSFPLDFHKGMSIEQVYSVVIHAGITVVTLSLPVVVGILVMGS
                     AASLLQTGLLWRANAVQPDVERISPIKGLSRLFSLRSVMELVKGLLKIAIVTGVGIWV
                     ARNDVLQIPGLIGFDLGTVLQVTGRLSLKVGLAVAGAIAILAGLDYFYQRYEWERSLR
                     MSKDEIKEEHKATEGDPLIKSRVRTIQREMTKKRMMAAVKTADVVITNPTHLAVALKY
                     DTATMGAPVVVAKGAGLVAERIRELARHHGVPVVEHKFVARTLFKLVDIGKEIPNELY
                     RAVAEILAFVYRARGVTSIRNS"
     gene            910..>2934
                     /gene="flhA"
                     /locus_tag="VN647_00115"
     CDS             910..>2934
                     /gene="flhA"
                     /locus_tag="VN647_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HWG95457.1"
                     /translation="MATAIEPVERNQLIKHPDVVISVGVVAILMVMLLPLPRFVLDLL
                     LSFDITLSVVILLVGLQVRRPIEFSVFPSVLLMITLFRLSLNIASTRLILLHGNEGAG
                     AAGEVIRAFGNFIVGGNYTVGLVVFSILVIINFVVVTKGAGRVAEVAARFTLDAMPGK
                     QMSIDADLNAGLINEADARRRRREIAEESDFYGAMDGASKFVRGDAIAAVIIILVNIV
                     GGLAIGILQQGMSPALAAQTYTILTVGEGLVAQIPALIVSTAAGIVVTRAASETDLGS
                     EMTRQLLMSSKPVGIAAGILLALGLVPGLPHVAFLALGSAIAWIAYQLHQQEQVQAAP
                     ILPPVTPKAEEGLTRVIPLDLMEVQVGYGLIGLVEGTQGTALLDRIKALRRQFAESMG
                     FVVPPIHIRDNLQLRPNEYAIILKGVEVAKADVLPGHLLAIDPGTGQRGLVQGIATKE
                     PAFGLPALWVPEEAREQAQMAGYTVVDASSAIATHLSELIKRHGHELLGRQEVQALLD
                     EVGKAHPKLVEELIPTLLPLGTVVRVLGNLLKEGIPIRDLRSILEAISDQATNTKDAE
                     VLTEYARQALARTITKQYQAPDGSLQVITLDPRLDRSLAEQAAALPPGAMLNLDPTLS
                     HKLLTGLKQAAERVAARGQQPIVLCSQVVRRHLRRHSDRTLHAVPVMGLNE"
BASE COUNT          583 a          769 c          908 g          674 t
ORIGIN      
        1 gcgctctcgt tccctctgga cttccacaaa gggatgtcga tcgagcaggt gtactcggtc
       61 gtcatccatg ccggcatcac agtggtgacg ctgagtctcc cggtcgtggt gggcattctg
      121 gtgatgggga gtgctgcgtc gctgctgcaa accgggctgt tgtggcgcgc caatgccgtt
      181 caaccggatg tcgagcgcat cagcccgatc aaggggcttt cccgcctgtt ctcgctgcgt
      241 tctgtgatgg agcttgtgaa ggggctgctc aagatcgcaa tcgtgacggg tgtcggcatt
      301 tgggtggcac ggaatgatgt actccagatc ccagggttga tcgggttcga cctcggtacc
      361 gtgctccaag taaccggacg gctctcgctg aaagtgggat tggccgtcgc gggggcgatc
      421 gcaattctgg ccgggctcga ctacttctac caacgctatg agtgggaacg aagcctgcgg
      481 atgtcgaagg atgagattaa ggaagaacac aaggccacgg aaggtgatcc gctcatcaag
      541 agtcgtgtgc ggaccattca gcgggagatg acgaagaagc gcatgatggc tgccgtgaag
      601 acggccgacg tggtgattac caacccgaca cacttggccg ttgctctgaa atatgacacc
      661 gccacgatgg gagctcctgt tgtggtcgcc aaaggggccg gcttggtcgc cgaacgtatt
      721 cgcgagctgg ctcgacatca cggagtaccg gtcgtcgaac ataaattcgt ggccagaacg
      781 ctgttcaaac tggtcgatat cggcaaagag attccaaatg agctataccg cgcggttgcg
      841 gagatcttag cgtttgtgta tcgcgccaga ggcgtgacct ccataagaaa ttcctaggat
      901 cagacagcga tggcgactgc aatagagccc gtggaacgaa accagttgat caagcatccg
      961 gacgtcgtca tatccgtcgg ggtagtggcc attctcatgg tgatgctgtt gccgttgcct
     1021 cgatttgtgc tcgatttgtt gttgagcttc gatatcacct tgtcggtcgt gatcttgctc
     1081 gtcggacttc aagtgcggcg tccgatcgag ttctcggtgt ttccgtcggt ccttctcatg
     1141 atcacattgt tcaggttgtc cctcaacatt gcttccacgc gtctcatttt gctgcatggc
     1201 aacgaagggg cgggggcggc cggagaagtt attcgggcct tcggaaactt tatcgtcggg
     1261 ggcaattaca cggtcggctt ggtggtgttc tccattctag tcatcattaa cttcgtcgtg
     1321 gtgacgaagg gcgccggacg tgtggcggaa gtcgccgccc ggttcacatt ggatgccatg
     1381 cccgggaagc agatgagcat tgatgcggat ctgaatgccg gtctgatcaa tgaggcggac
     1441 gcgcgccgcc ggagacgtga aattgcggaa gagtcggact tttacggtgc gatggacggt
     1501 gccagcaagt ttgttcgagg agatgccatt gcagccgtca tcatcatact cgtcaatatc
     1561 gtcggtggat tggcgatcgg tatcctccaa cagggcatga gtccggcact tgcggctcaa
     1621 acctatacga tactgacggt cggtgagggg ttggtggcgc agattcccgc cctgatcgtt
     1681 tcgactgccg ccggtatcgt cgtgactcgc gccgcttcgg aaacggattt ggggagcgag
     1741 atgactcgac agctgttgat gtcttccaag ccggtgggca ttgctgcggg tattcttctc
     1801 gcgcttggat tggtccctgg gcttccgcat gtggcgttct tagcgttagg gagcgctatt
     1861 gcctggattg cctaccaact tcatcagcag gaacaggtcc aagcagctcc gattcttccc
     1921 cctgtcactc cgaaggccga ggaaggcttg acccgagtca ttccgctcga ccttatggaa
     1981 gttcaggtgg ggtacggctt gataggactc gttgagggaa cgcaaggcac cgcactgctc
     2041 gatcggatta aagcgctccg gcgacaattt gcggaatcga tgggctttgt cgtgcctccc
     2101 attcatatac gggacaatct tcagttgcgc cccaacgaat atgccatcat tttgaagggc
     2161 gtggaagtgg ccaaggctga tgtcttgccg gggcatctgt tggcgatcga tcctggtacc
     2221 ggccagcggg gtttggtgca agggatcgcg accaaggagc ctgccttcgg attgccggca
     2281 ctctgggtcc ctgaagaggc cagggaacag gctcaaatgg ccgggtatac cgtggttgat
     2341 gcgagttcag cgatcgccac acatctttct gaattgatta agcgtcatgg ccatgagttg
     2401 ttgggacggc aggaggttca agcactgttg gacgaagttg gaaaagcgca tccgaagctg
     2461 gtggaggagc tcattcctac gctcttgccg ctcggaacgg tcgtcagggt tcttggaaac
     2521 ctgctcaagg agggtattcc tattcgagac ctacgatcga ttctcgaggc catttctgat
     2581 caggccacca acaccaagga tgcggaagtc ctcacggagt atgcgaggca ggcgttggcc
     2641 cggaccatca ccaaacagta tcaagcgcct gacggcagtt tgcaggtcat taccttggac
     2701 ccccgactgg atcgatcatt ggcggaacaa gcggctgcat tgcctcccgg ggccatgttg
     2761 aaccttgatc ccaccctctc gcacaagctg ttaaccggac tcaaacaagc cgccgaacgg
     2821 gttgcggctc gggggcagca gccgatcgtg ctgtgctcac aggttgttcg acgccatctc
     2881 cgtcgacaca gcgatcgtac gctgcatgcc gtgcccgtga tgggactcaa tgaa
//
LOCUS       DATNDD010000006         3291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1994565, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000006 DATNDD010000000
VERSION     DATNDD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994565"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VN647_00120"
     CDS             complement(<1..411)
                     /locus_tag="VN647_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWG95458.1"
                     /translation="MSLSDRLTEDLKLAMKSRDQLRMDVIRMIKAAILNKEVELKRDL
                     DDAEMSRVMTTLVKQRRESVEQFEKAHRTELAAKERKEIEIIESYLPKPLSPQEIEGI
                     VSSAVTETGSHSLKDMGTVMKAVMARLAGQSVDGK"
     gene            563..784
                     /locus_tag="VN647_00125"
     CDS             563..784
                     /locus_tag="VN647_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95459.1"
                     /translation="MPKKVTWRMGQKHGLIATGIGMLLLATEGCAGEARKPAPTMTQD
                     QVREHADKTFDKLKQEEKNRPMGSGVGPY"
     gene            complement(778..2187)
                     /locus_tag="VN647_00130"
     CDS             complement(778..2187)
                     /locus_tag="VN647_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG95460.1"
                     /translation="MTVQPELSHSAEQVPGIHHPTSTHPHESGRRFGVRDGLFQAVAQ
                     GAGEQYLSAFALLLHATPFHLSVLSAIPQLFGTWAQLVSVKVAHWFPNRASQVFWGII
                     GQSIAWIPILALPLLWPHKGPWLLIAAVAVYFTFSHFTSPVWNSLITDLLEPNERGMY
                     FARRARTIAMTSFVALCLAGALLSFFEQQQLLWVGFAVMFLIAGLSRSASAFLLMKVS
                     DLPPHGPNSNSTGFLVFLRTGMSENFRRFLLFSGLMHSAVLVAGPFFVIYLLQDLHLA
                     HWQYGTWLAAGIIGQFLTLPAWGQFGDRFGNKALLSFTGLLVAFLPMLYLCGTTWPFL
                     VTVNFFGGMVWAGLGLGLNNYVFDAVQPVDRAKAVAVSSIVNAIGWTMGTVIGSWLIS
                     TVPTNLHVGALTLEPPSNLPFIFFLSGLLRLIVSATLLRTFHEPRQVEQRAHHRLVWE
                     LPLLKPLRQLSRRPTIANQ"
     gene            2362..2646
                     /locus_tag="VN647_00135"
     CDS             2362..2646
                     /locus_tag="VN647_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95461.1"
                     /translation="MGDRVRITDSEKLSLLYERFRDVCLVEKEVWKEIFMPREVMHGP
                     VRTNIQDRYEVAVDDPAAEQTIENAILRGSAALGAAIQECRAHILFFKRS"
     gene            complement(2647..>3291)
                     /locus_tag="VN647_00140"
     CDS             complement(2647..>3291)
                     /locus_tag="VN647_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HWG95462.1"
                     /translation="QPRSRAEQVVYGSVQEIAQRLHITMPEVWVYDSPDPNAFATGPS
                     KNNSMVAVSTGLLQNLREDEVKAVLAHEMGHVYNGDMFATTVLAGLMNTFVYYIAMWV
                     QRFFAERDQAALGFGLSLVLQIVVSILASIVISWFSRHREFGADAFAARVYGKDSMIG
                     ALRAIDRWVNRAQFEYSTQDALATMKISGKTGGVMSLFSTHPSIDARIAALEAL"
BASE COUNT          826 a          792 c          972 g          701 t
ORIGIN      
        1 cttgccgtca acggattgtc cggcaaggcg ggccataaca gccttcatga ccgttcccat
       61 atctttgagt gagtgggaac cggtctccgt gacagccgat gagacaatgc cctcaatctc
      121 ttgaggggaa agcggtttgg ggaggtagga ctcgataatt tcgatttcct tccgttcttt
      181 ggcagccagt tccgtccggt gagctttttc aaattgctcg acggattccc tccgctgttt
      241 tacgagggtc gtcatcacgc ggctcatttc agcatcatca aggtcccgct ttagctcgac
      301 ttctttgttc aggatggcgg ctttgatcat acggatgaca tccatgcgaa gttgatcgcg
      361 tgatttcatc gcgagcttga ggtcctcggt caggcggtcg gacaacgaca tgggaagtct
      421 cacgggttat gcgctgagcc aggataggag cataccctct tggtttcaac gagtcaacga
      481 gacgagttgg aaagtgttca gacgagaaat ggtatgatgt ggctcgaatc gtatgacgat
      541 catgacgaaa ggaggattga gtatgccaaa gaaggtgaca tggcggatgg gacagaagca
      601 tgggttgatt gcgactggaa tagggatgtt gttgttggct accgaaggct gtgctggcga
      661 agctcgtaaa cctgcaccga cgatgacgca ggatcaggtc cgcgagcatg ccgataagac
      721 gtttgacaag ctgaaacaag aagagaagaa tcgtccaatg ggttctgggg tgggtcctta
      781 ctgattggcg atggtcggtc ggcgtgagag ttgtcgtagg ggtttcaaca gcggcagttc
      841 ccataccaat cggtgatggg cgcgttgttc cacctgacgc ggttcatgaa aggtccggag
      901 aagcgttgct gacacgatca agcgtaatag accggagaga aagaagataa acgggagatt
      961 ggatggaggc tccagagtca aagctccaac atgcagattg gttgggacgg tgctgatcag
     1021 ccagcttccg atgactgttc ccatggtcca cccgattgcg ttgacgatgc ttgaaacggc
     1081 gaccgccttt gcgcgatcca ccggctgtac cgcatcaaag acataattat tcagcccaag
     1141 ccccagccca gcccagacca tgcctccgaa gaaatttacc gtgacaagaa acggccaggt
     1201 cgtcccgcac aggtacagca tcggcaggaa cgcgacgagc aggccagtga aagaaagcaa
     1261 tgccttgttt ccaaaacgat caccgaattg tccccaggcc ggcaaggtta ggaattgccc
     1321 gatgatgccg gcagcgagcc aggttccata ttgccaatga gcgaggtgaa gatcctggag
     1381 cagatagatg acgaagaacg gaccggcaac cagcacagca gaatgcatga gcccggaaaa
     1441 cagtaggaag cggcgaaaat tttcagacat gcccgtacga agaaagacta aaaagcccgt
     1501 tgagttgctg ttcggcccat gcggtggtaa gtcgcttacc ttcatcagca agaaagccga
     1561 cgcactacgg gagagtccgg ctatgaggaa catcacggca aagccgaccc aaagaagttg
     1621 ctgttgctcg aagaagctca atagagctcc agccaagcag agtgcaacaa agctggtcat
     1681 tgcaatggtc cgcgctcgcc tggcgaagta catgccccgt tcgttgggct cgagcaggtc
     1741 agtgatgaga ctattccaca caggagatgt gaaatgagag aaggtgaaat acacggcgac
     1801 ggctgcgatg agcaaccacg gccctttgtg aggccagagc aacggcaacg caagaatggg
     1861 gatccaggcg attgattgtc cgatgatgcc ccagaaaact tgtgaggctc ggttggggaa
     1921 ccagtgagcg actttgacgg aaaccaattg agcccatgtg ccaaagagtt gaggaatggc
     1981 agagagaacg ctcaagtgga atggtgttgc gtggagaagt agggcaaagg ccgagagata
     2041 ctgctcgccg gcgccttggg cgacggcttg gaacagcccg tcacgaacgc cgaatcgccg
     2101 accggattcg tgtgggtgtg tggaagtagg atggtggatg ccgggaacct gctctgctga
     2161 atgtgacaat tctggttgga ccgtcataat ccgaaagcca agagtactct accatagaac
     2221 agcaaacgca caaggattca tcggatcatg gcgggtagtg attgtcccct gcgatgtctc
     2281 tcagtatgat gcttctatga ctgatggtgt ttcggcagga atattgtcaa tcggtgagat
     2341 aattcaggaa gaaagggact catgggagat cgcgttagga ttaccgactc tgaaaagttg
     2401 agcttactgt acgagcgatt tcgggatgtc tgtctggtcg aaaaagaagt ctggaaggaa
     2461 atcttcatgc ctcgcgaggt aatgcatggg ccggttcgga cgaatatcca ggatcgatat
     2521 gaggttgcgg ttgacgatcc tgccgctgaa cagacgattg agaacgccat ccttcgaggg
     2581 agtgccgcat tgggcgcggc gattcaagag tgccgcgccc acattttgtt ctttaagaga
     2641 tcatgatcag agcgcttcca atgccgcgat ccgtgcgtca atcgatgggt gagtcgagaa
     2701 caagctcatg accccaccgg tttttccgga aattttcatg gttgccagtg cgtcctgtgt
     2761 cgagtattcg aactgagcac gattgaccca gcgatcaatc gcccttagcg cgccgatcat
     2821 ggagtccttc ccatagaccc gagccgcaaa cgcgtcggct ccgaactcgc gatggcgtga
     2881 gaaccagctg atgacgatag aggccaaaat actgacgacg atctgaagga ccagtgatag
     2941 gccgaatccc aatgccgctt gatctcgttc cgcgaagaaa cgttgcaccc acatggcgat
     3001 gtaatacaca aaggtattca tcagcccagc caggacggtc gtggcgaaca tgtcgccgtt
     3061 atacacatga cccatttcgt gagccagaac cgccttgact tcgtcttctc tcaagttctg
     3121 aagcaaaccg gttgaaacgg ccaccatgga attgtttttg ctgggtccgg tcgcgaacgc
     3181 attggggtcc ggtgaatcat agacccacac ttcgggcatt gtgatgtgca ggcgttgagc
     3241 gatttcctgt acactgccgt aaacgacctg ttcagcgcgt gagcggggtt g
//
LOCUS       DATNDD010000007         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_562559, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000007 DATNDD010000000
VERSION     DATNDD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562559"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2540)
                     /gene="metH"
                     /locus_tag="VN647_00145"
     CDS             complement(<1..>2540)
                     /gene="metH"
                     /locus_tag="VN647_00145"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWG95463.1"
                     /translation="LLDGGADLLLVETIFDTLNAKAAFFAIQQAFASGARQVPVMASV
                     TFIQAGSNRGVTGQTVEAFWNSISHVPLLSIGMNCALGPKEMRPLIEELSQIAPIYVS
                     AHPNAGLPNPLLPTGFPETPETLAPQLREWAENGWLNIVGGCCGTTPAHIKRIAEAVR
                     GMKPHALSKVEPYTRLSGLEAVTIRPDSNFINIGERTNVTGSPAFAKLILADDYEAAL
                     SVARQQVEGGAQIIDINMDEGMLDSKAAMEKFLRLVASEPDICKVPIMVDSSKWEVLE
                     TGLKNIQGKAVVNSISLKEGEEKFVDQATLIRRYGAAVVVMAFDEKGQADTLERKKEI
                     CARSYKILTERVGFPPQDIIFDPNILTVATGIEEHNNYAVNFIEATRWIKQNLPRAKV
                     SGGISNISFSFRGNNVVREAMHAAFLYHAIKAGLDMGIVNAGQLAVYEEVPKDLLDLV
                     EDVLLNRRPDATERLVNFAETVKAKGKAAVKDDEWRSGTVEERLSHALIKGITDYIDL
                     DTEEARRKYSKPLEVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLMPF
                     MEEEKKRSGNFQAQGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPCEKIL
                     AAARENKPDIIGLSGLITPSLDEMVHVAKEMTREGFDLPLLIGGATTSKAHTAVKIAP
                     SYEPGVVHVLDASRAVGVVGSLVSRTQKQDFVKQVRADYERVRQSHHDRGAKPLISIA
                     QARANRFMPDWEKIDIPTPATLGARTILDQSLVELIPYIDWSPFFHTWELRGRYPTIF
                     EDPTVGPKAKELYDDARRLLDEIVQKQQLKAKGVYGFFPAASVGDDIEL"
BASE COUNT          536 a          765 c          659 g          580 t
ORIGIN      
        1 agttcaatgt cgtcaccgac cgacgcagca ggaaagaatc cgtagacccc cttggctttg
       61 agctgttgtt tctggacgat ctcatcgagg aggcgccgcg catcgtcata caactccttg
      121 gcttttggcc caacggttgg atcctcaaaa atcgtcgggt agcgtcctct taattcccac
      181 gtatggaaga agggtgacca gtcgatataa ggaatgagtt caaccaacga ctgatcgagg
      241 atcgtccgag cgcctaatgt cgccggcgtg ggaatatcaa tcttctccca gtccggcatg
      301 aaccgattgg cgcgcgcttg ggcgatcgag ataagcggct tcgcaccacg atcgtgatgt
      361 gattgcctca cccgctcata gtcggcccgc acttgtttca cgaaatcttg cttctgcgtt
      421 cggctgacca ggcttcccac gacgcccacg gcgcgtgatg cgtccaacac atggaccacg
      481 cccggctcat aagagggcgc gatcttgacc gccgtgtgag ccttgctggt cgtggcacca
      541 ccgatcaaga gaggcaggtc gaacccctcg cgcgtcattt cttttgccac atggaccatc
      601 tcgtctagcg aaggagtgat gagcccgctc aagccgatga tgtcgggttt attctcccga
      661 gcagcggcca gaatcttttc gcatggtacc atcactccca ggtcgatgac ttcatagttg
      721 ttgcagccaa gcacgacgcc gacgatgttt ttcccgatgt catggacatc acccttgacg
      781 gtcgcgagta ggactttccc ctgtgcctgg aaattgccgg accgcttctt ctcttcttcc
      841 ataaagggca tcagatgggc gacggccttc ttcatgacgc gggcgctctt gacgacctga
      901 ggcaaaaaca tcttgcctga gccgaacaga tcgccgacga cgttcatacc ggccatcaac
      961 ggtccctcga tcacttccaa aggcttggaa tattttcgcc gggcctcctc cgtatccagg
     1021 tcgatatagt ccgtgatgcc cttgatgagc gcatgggata accgttcctc aaccgtccca
     1081 ctacgccact catcgtcttt gacagccgct tttcctttcg ctttcacggt ttccgcgaaa
     1141 ttcaccaggc gttcggtggc gtccggccgt cggttgagca acacatcctc tacgagatca
     1201 agcaggtctt tgggaacttc ttcgtaaact gccagttgcc cggcgttgac aatacccatg
     1261 tcgagaccgg ccttgatggc gtgatacaaa aaggccgcat gcatcgcttc ccgaaccacg
     1321 ttgttacccc gaaacgaaaa agaaatattg ctgatgcccc cgctgacctt cgcacgcggc
     1381 aagttctgct tgatccagcg cgtcgcctcg atgaaattca cggcgtagtt gttgtgctcc
     1441 tcgatccccg ttgcgacagt cagtatgttg ggatcgaaga taatatcttg cggaggaaac
     1501 ccgacgcgct ccgtgagaat cttatatgag cgggcacaga tctctttctt tcgttccaga
     1561 gtgtcggcct gacctttctc atcgaacgcc atgacaacaa ccgcagcacc gtagcgacgg
     1621 atgagcgtcg cctgatcgac gaatttctcc tcgccttcct taaggctgat actgttgacg
     1681 acggctttac cttggatatt tttcagaccc gtctccaata cctcccactt ggagctgtcg
     1741 accatgatgg gcactttgca gatatccggc tccgacgcaa cgagacgaag gaacttctcc
     1801 atcgcagcct tggaatccaa cataccctca tccatgttga tatcgataat ctgcgctccg
     1861 ccctccactt gctgccgcgc caccgataga gccgcctcat aatcatcggc aagaatcagc
     1921 tttgcgaaag ccggtgagcc ggtcacgttc gttcgttcac cgatattgat gaagttcgaa
     1981 tcgggccgaa tggtgacggc ttcaagaccg ctcaatcgcg tgtacggctc aaccttcgag
     2041 agtgcatgcg gcttcatccc acgcacagct tcggcgatgc gcttgatatg ggccggtgtt
     2101 gtgccgcaac agccgcccac gatgttcaac cacccgtttt ccgcccattc gcgtaattga
     2161 ggcgctaagg tttccggcgt ctcaggaaac ccggtgggca acaagggatt cggcaagccg
     2221 gcattgggat gggcgctgac gtagatcggc gcgatctgcg acaattcttc gatcagagga
     2281 cgcatttctt ttggaccgag cgcgcagttc atccctatgc tcaacagcgg cacatgagag
     2341 atggaattcc agaacgcttc gacggtctgc ccggtcacgc cgcgattgct gccggcctgg
     2401 atgaacgtca ccgatgccat gaccggaacc tggcgcgcgc cggacgcgaa cgcctgttgg
     2461 atcgcaaaaa atgcggcctt cgcattcaac gtgtcgaaga tcgtctccac cagcagaaga
     2521 tcggcgccac cgtcgagcaa
//
LOCUS       DATNDD010000008         4255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3733015, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000008 DATNDD010000000
VERSION     DATNDD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733015"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VN647_00150"
     CDS             <1..435
                     /locus_tag="VN647_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWG95464.1"
                     /translation="LCGEVHDQNAAALGGEAGHAGLFGTADAVLAITGEWLKAYHGRK
                     AILDQGIAREFTRRQKPEGSSSWALGWDTPSTPSSAGRYFGARSFGHLGYTGTSVWID
                     PVRELEVVLLSNRVHPSSKNEAIREFRPAIHDLVYQEFIGSI"
     gene            complement(432..749)
                     /locus_tag="VN647_00155"
     CDS             complement(432..749)
                     /locus_tag="VN647_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95465.1"
                     /translation="MSTLPLMFKKEGLVEKHQLEGVDPSDRYFNRTVLVNRIPSGYSA
                     RIMYEAFVVESQSHPTIAAAVKELVAKLQDSGFTRMRTRANFKGTRYLAEKETWLDYP
                     DRS"
     gene            905..1447
                     /locus_tag="VN647_00160"
     CDS             905..1447
                     /locus_tag="VN647_00160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWG95466.1"
                     /translation="MNIPNSLTILRILLIPVYIGFMTYGSYGFALLTLLVAGLTDAID
                     GHLARKLNQRTRLGSLLDPLADKLLLTSSFISLAMLHLVPSWLVILVVSRDIILLLGT
                     VVAHVTGTPINVTPTFLGKGTTLLQLSYVLLIVLLTWRELDRSMLAPLVVLMVGFTLA
                     SGLHYLYRGYQDTNAAPPLA"
     gene            1594..2808
                     /locus_tag="VN647_00165"
     CDS             1594..2808
                     /locus_tag="VN647_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWG95467.1"
                     /translation="MATFTYVGRTKSGAVKKGELVAKSRDEAVDQLRKQSVVVTSLEE
                     KAAKEGFSFKLGSGVSEKDLVVFTRQFGTMINAGLPLIQCLEILSTQSENAALRKSVG
                     EIKVQVEGGSTFSDALRKHPKIFDDLYVNMVHAGEVGGLLDTILGRLSKHIEKAMKLK
                     GQIKSAMVYPAAIVGIAAIVITVLMIWVIPVFEKMFKEMSGGKMALPGPTQLVIDMSN
                     FAQGNWYIILGAIVALVVTVKKYYATPRGKLAIDKLLLKLPVFGDLIRKASVAKFTRT
                     LGTLLASGVPLLEALTICAKTSGNKVVEGALLDAKVSISGGKTIAEPLAKSGTFPKMV
                     THMISVGESTGALDSMLGKIADFYEDEVDQAVTNLTALLEPMMMVFLGVTVGFIVVAM
                     YLPIFTMASAIG"
     gene            3018..>4255
                     /locus_tag="VN647_00170"
     CDS             3018..>4255
                     /locus_tag="VN647_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG95468.1"
                     /translation="MGDIKARIYWLMGWRVVLVTLLLGLSLAFEVTKGERVETFYALI
                     IFTYAVTILYAFLFRRFTTPQVLVQFAWVQIAVDFLLETVLIARTGGIESPFAVLYVI
                     SVTVASLVPRRRVGLLTASLCIILFGILTNVQLYGLAEAWGWLPHANLSAAETLHAFG
                     VYGLAFLVVGFLSGALADQLGLADQSIREKEQGISRLQAFHENIVCSISSGVFTTDEE
                     GRITSFNPAAQEATGYGFDQVEGHPWREVFAWHPRQQDEDVSDNMRFEVECKRSDGNR
                     LVLGMTLAPLHERGEKTGLVGVFKDLTQIRDLEEEMQRKEWLASLGEMSAGMAHEIRN
                     PLGALAGAMQMLRKDFEADETSQRLIEIAVREATRLDAIITEFLQYARPPALNLAEYD
                     LNKVLAETLDLVQHEARNRT"
BASE COUNT          971 a         1055 c         1180 g         1049 t
ORIGIN      
        1 ttgtgcggag aagtgcacga tcagaatgca gccgctttgg gcggcgaagc cggtcacgcc
       61 ggactgtttg ggactgccga cgcggtatta gctattacgg gtgaatggtt aaaagcttac
      121 catggtcgaa aggctatcct cgatcaagga attgcgcggg agtttacacg gcgacagaaa
      181 ccggaaggat cttccagttg ggcgcttggg tgggatacac catcaacgcc gtcatctgcc
      241 ggacgatatt tcggagcgcg atcgttcggt catctcggct atacggggac ttccgtctgg
      301 atcgatccag tgcgagaatt ggaagttgtg ttgctctcca atcgagtgca tccttcgagt
      361 aagaatgaag caatccggga attccgtcct gcgattcatg atttggtgta tcaggaattc
      421 atcggctcga tttaagaccg gtctggatag tcgagccacg tttctttttc ggcaaggtag
      481 cgtgtcccct tgaaattagc ccgcgttctc atgcgagtga atccggagtc ttgaagttta
      541 gccactaatt cctttaccgc tgctgcaatg gtagggtgtg actgactttc aacaacgaaa
      601 gcttcataca tgatcctagc cgagtatccc gatggaatcc gattgaccag aacggtgcgg
      661 ttgaaatagc gatcgctcgg atcaacccct tcaagctgat gtttctcgac caatccttct
      721 ttcttaaaca tcaatggcaa cgtgctcatg ctttcttacc cctccgaaat aacagttgac
      781 gacaagccgt aatgacttaa ttataggagg tgattcgcgc aacctgcaac tcattgacaa
      841 gcgtggcttc gattactatg atgcctcccc ctcatccagt cagaggttcg tccggctcca
      901 tctcatgaac attcccaata gcctcacgat tcttcgaatc ctcttgattc ctgtctacat
      961 aggattcatg acctacggtt cgtatgggtt cgcgctgctg acactcctcg tcgcaggact
     1021 gacggatgcc attgacggtc atctggcgcg taagctgaat cagcggacac gattggggag
     1081 tttacttgat ccccttgcag acaagctgct gctgacgtcg agtttcatct cgctggcgat
     1141 gctgcacctg gtgccgtcct ggcttgtcat cttggtcgtg agccgggaca ttattctcct
     1201 gttggggact gtggtggctc acgtcaccgg tacacccatt aatgtgactc cgacgttctt
     1261 gggcaaagga acgactctcc ttcagttgag ctatgtcctt ttgattgttc tcttgacatg
     1321 gcgagagctc gaccgctcca tgcttgctcc actcgtcgtg ctgatggttg ggttcaccct
     1381 tgcctcaggg ttacattacc tttatagagg atatcaagac acgaatgcgg ctcctccgct
     1441 tgcctagtga ccgctgttgc gcatacgacc tcttcagagc tcggtaaata attcaattgt
     1501 ttttgacagg tttccgaccc accagtagac tcgtttcgta gtcctagcct tccgtacaat
     1561 cggctcaggg agtcacaacc aggacgagaa tctatggcca cgtttacata tgtggggcga
     1621 accaaatctg gagcggtgaa aaaaggcgaa cttgtcgcaa agtctcgcga cgaagctgtg
     1681 gaccaactac gcaaacaaag cgtggtggtg acaagtcttg aagaaaaagc ggccaaagaa
     1741 gggttcagct ttaagctcgg gagcggagtg agcgaaaagg acttggtcgt ttttaccagg
     1801 caattcggga ccatgatcaa tgccggcttg cccttgattc aatgcctgga aatcttatcg
     1861 acgcaatcgg agaatgcggc tctgagaaaa tccgtgggtg agatcaaagt ccaggttgag
     1921 ggaggttcga cattttcaga tgccctccgc aaacacccaa aaatcttcga tgatctgtac
     1981 gtcaatatgg tccatgccgg tgaagtcgga gggctgctgg ataccattct tggtcgtctc
     2041 tcgaagcata tcgagaaggc catgaagttg aagggacaaa tcaagagcgc aatggtctat
     2101 cctgccgcca tcgtcggcat tgcggctatc gtgatcacgg tcctgatgat ttgggttatc
     2161 cccgtattcg aaaaaatgtt caaggaaatg tccggcggga aaatggcgct tccaggaccg
     2221 acgcagcttg tcatcgacat gagtaatttc gcccaaggaa attggtacat catccttggg
     2281 gcgatcgtcg cgcttgtggt gacggtcaaa aaatactatg cgactccacg aggcaagttg
     2341 gcgatcgata aactcttgtt aaaactgccg gtgtttggag atctcatcag aaaagcatcg
     2401 gtcgccaaat tcacacggac gttaggaact ctgctggcga gcggggttcc gctgctggaa
     2461 gcattgacga tttgcgcgaa gacctccgga aacaaagtgg tagagggagc gctccttgat
     2521 gccaaagtca gtatcagtgg aggaaagacg atcgctgaac cgctcgccaa aagcgggaca
     2581 ttccccaaga tggtaaccca catgatttcg gtcggtgaat cgacgggcgc gctggatagc
     2641 atgctcggga aaatcgccga tttttacgaa gacgaggtgg atcaggccgt gacgaatctc
     2701 accgccctcc tggagccgat gatgatggtg ttcttgggtg tcaccgtcgg atttatcgtg
     2761 gtcgcgatgt atctaccgat ctttacgatg gcgtccgcaa tcgggtaggc aaatcgacga
     2821 ttcgatcttg cgaagcggct tcgtccctcc cctcaagcga aagggatgaa gccgctgact
     2881 tctcggcgag ccccctttat cgttacgata aagagcgacg tacttcccaa gacataatag
     2941 tggtgtcggt cgatacctcc cgcgaagtta ggcgggacgt catggtgagg accatcttgt
     3001 aggaggagtt tcgtttcgtg ggagatatca aagccagaat ttactggttg atgggatggc
     3061 gagtcgttct tgtgacgctt ctcctggggc tgtccctcgc gttcgaggtg accaagggag
     3121 aacgcgttga aacgttctac gccctgatca ttttcaccta tgcggttacg attctctatg
     3181 cctttctgtt ccggcggttc accactcctc aagttctcgt gcaattcgca tgggttcaga
     3241 ttgcggtcga ttttttgctc gagacggtat taatcgcaag gacgggcgga attgaaagcc
     3301 cgtttgccgt gctctatgtg atcagcgtaa cggtcgcaag tttggtcccg cgtcgccgcg
     3361 tgggcctttt gacggccagc ctctgcatca tcttgtttgg gattttgacc aatgtgcagc
     3421 tttacggtct tgctgaagca tggggatggt tgccccacgc taacctcagt gccgctgaga
     3481 cgctccatgc attcggagtc tacggcctgg cctttctggt ggtcggtttt ttgagtggcg
     3541 cccttgcaga tcagctcggg cttgccgacc agtcgatccg tgaaaaagaa caggggatca
     3601 gccgccttca ggcgtttcat gaaaacatcg tttgcagtat cagcagcggc gtgtttacga
     3661 ccgacgagga aggacggatc acatcgttca atcctgcggc acaggaagcc acaggctacg
     3721 gctttgacca ggtagagggg catccctggc gggaggtgtt cgcctggcat ccccgtcaac
     3781 aagacgaaga tgtctccgat aacatgcggt ttgaagtgga gtgcaagcgg tcagacggca
     3841 atcggttggt tcttggcatg acgctcgcgc ctttgcacga gcgaggagag aaaacgggtc
     3901 tggttggagt gttcaaggat ctcactcaga tccgcgacct ggaagaagaa atgcagcgca
     3961 aggagtggct tgccagtctt ggagaaatgt cggcggggat ggcccatgaa atcaggaatc
     4021 ccttgggtgc actggctgga gcaatgcaaa tgcttcggaa agatttcgag gccgatgaga
     4081 ccagccaacg cctcatcgag attgcagttc gggaagcgac ccgattggat gccattatca
     4141 cggagtttct tcagtatgcc aggccgcccg cactcaattt ggctgagtac gatttaaaca
     4201 aagtccttgc tgagactctt gatctggtac aacatgaagc acgaaacaga acgaa
//
LOCUS       DATNDD010000009         3800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2571363, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000009 DATNDD010000000
VERSION     DATNDD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571363"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VN647_00175"
     CDS             complement(<1..250)
                     /locus_tag="VN647_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95469.1"
                     /translation="MPSLDVQNPEMPDLQFVLFVSALCTADLTTINVPQELRQTIFDR
                     CWALLHTDAPPTNPKERVLDLRGGTELTLDACASTIRSL"
     gene            340..615
                     /locus_tag="VN647_00180"
     CDS             340..615
                     /locus_tag="VN647_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWG95470.1"
                     /translation="MVTIVLTGQLQTLDGECDLACEVDGSMSVRQLIQRQGVQLRHLL
                     QLLREKKVLVTINKKIASEDSPVRDGDAIRLIGHDGMGSSGLGPSHP"
     gene            complement(742..1029)
                     /locus_tag="VN647_00185"
     CDS             complement(742..1029)
                     /locus_tag="VN647_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWG95471.1"
                     /translation="MAKSMTKSQIADYLAGKAGITKKGAVQILDDLAALAYREAKNVF
                     TVPGIGKLKLANRKARIGRNPQTGEEIKIPAKRVVKFRVAKAAKDSILGKK"
     gene            1321..1881
                     /locus_tag="VN647_00190"
     CDS             1321..1881
                     /locus_tag="VN647_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95472.1"
                     /translation="MGKDLPILPLGAVGQAPEQLGSFFYSRVFCQKENSPPLRLLIDF
                     LKSRGQSPIVPPNLEKGALEEWAWVQVALSYHRERKPVHLFCVRDRGSYRDVYDQEKG
                     FFLDALTAHSDIEAQLAMEYVTRCRFILTTRMAENDVTDEGYDFNGWILEFYQEQCNG
                     IVQIDKQGFYSPKGELIVDMSVSGED"
     gene            2401..2541
                     /locus_tag="VN647_00195"
     CDS             2401..2541
                     /locus_tag="VN647_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95473.1"
                     /translation="MEAVEIEALRLQAGIMGEELFLPYAFIRDPDVGELEFGLGQRRI
                     PN"
     gene            2610..2686
                     /locus_tag="VN647_00200"
     tRNA            2610..2686
                     /locus_tag="VN647_00200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2644..2646,aa:Arg,seq:ccg)
     gene            2779..2854
                     /locus_tag="VN647_00205"
     tRNA            2779..2854
                     /locus_tag="VN647_00205"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2812..2814,aa:Lys,seq:ctt)
     gene            complement(2861..3214)
                     /locus_tag="VN647_00210"
     CDS             complement(2861..3214)
                     /locus_tag="VN647_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95474.1"
                     /translation="MHILNQCKKRLDLLFMAAEHRSKVDASLMRLAVEDVRSDLEEGS
                     ELRGHIDLTQELFAHSERSICWGINRPVMTQRYAHHYPESLRDWIDLLDRLVGQGTEL
                     VHPGQVSAGVRRVSD"
     gene            3446..3625
                     /locus_tag="VN647_00215"
     CDS             3446..3625
                     /locus_tag="VN647_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95475.1"
                     /translation="MATKGTNPPEKRRKLFGLSLDRQLMLEVQHLALDQDRYVNEMIE
                     EAAKDLLKKYREKAK"
BASE COUNT          865 a          946 c         1076 g          913 t
ORIGIN      
        1 gaagcgatcg gatcgtggac gcgcaggcat cgagtgtgag ctccgttccc cctcgtagat
       61 caagaacacg ttctttagga ttggtgggtg gcgcgtctgt gtgaaggagg gcccagcagc
      121 ggtcaaagat ggtctgtcgc agttcttgcg ggacattgat cgtcgtcaga tccgctgtgc
      181 agagtgccga cacaaacagg acgaattgga ggtcgggcat ttcgggattt tgaacatcga
      241 gagatggcat gactaccatt atcaggaggg aatgcatggc gagtcaactc tcgtaaaatg
      301 aagaggttgg aacgtttgca tatatgagga acggcggata tggtgaccat tgtattgacc
      361 ggacaattac aaactctgga tggcgaatgt gatctggcat gcgaagttga tgggtcgatg
      421 tcggttcgcc aactaatcca gcgacaaggc gttcagcttc ggcatctgct gcagctcctg
      481 cgggagaaga aagtgctcgt gaccatcaat aagaagatcg ccagcgaaga ttcacccgtt
      541 cgggatgggg acgcgattcg cctgatcgga cacgacggca tggggagtag cgggcttggg
      601 ccgtcgcacc cctaattgtg taggtgaatg aagagagggg tcgagattcc ctgagtctcg
      661 acccctcttg tatcttctaa aggcagcgga atgagtccaa actgacatgg atcaggcgct
      721 gcgattgtgc agggataatc cttatttctt gccgagaatc gagtccttcg cggccttagc
      781 gacacggaac ttgaccactc gttttgccgg gatcttgatt tcctcaccgg tttgaggatt
      841 gcgcccgatg cgcgccttac ggttagcgag cttcagctta ccgatcccgg gcactgtgaa
      901 gacatttttg gcttcacgat aggccagggc cgccagatca tcaagaatct gaaccgcccc
      961 tttcttcgtg atgccggctt tcccggcgag gtaatctgca atctgagatt tcgtcattga
     1021 ttttgccatt cgtgaacctc cttgggggta agggataaag taagcggata tgtcgcctga
     1081 cgcgcctcgc gtaaacacat cctggctggt tttgggtcgc cggtgtgtgt gttcgtactc
     1141 acatcgtgaa aacccgcgag agtgtagcca agagtcggaa gagtgtcaac gagaaaaacg
     1201 cgcgtctggc gtggagaaat gcacacacta ccccttgcgg tttaagcaaa cggaagggta
     1261 gagtagagct gtatggacct cataatcgat gtaaacggct cttgacgatg gtgaaagtgc
     1321 atgggtaaag acctgcctat attaccctta ggtgcagttg gtcaagctcc ggaacagctt
     1381 ggaagctttt tctattcgcg agtattctgc caaaaagaaa actcgcctcc gctccggctg
     1441 ttgatcgatt ttctgaagtc ccggggccaa agtcctattg tcccccccaa tctggagaaa
     1501 ggagcgctgg aggaatgggc atgggttcaa gtggccttga gttaccatcg agagcgtaag
     1561 cccgttcacc tcttttgcgt acgtgatcgc ggcagctatc gagatgtcta cgatcaggaa
     1621 aagggatttt ttctggacgc cctcaccgct cactccgata tcgaagcaca actcgcgatg
     1681 gaatatgtga ctcgttgtcg gtttatcctg acgacgcgta tggcggagaa tgatgtgacc
     1741 gacgaaggct atgatttcaa tggatggatc ctcgagttct atcaggagca gtgcaacgga
     1801 atcgtccaaa tcgacaagca aggtttctac tcacccaaag gtgagctcat cgtggatatg
     1861 tcggtctcgg gcgaagacta acgtgccggg gacgtttccc tttccttcaa cgttgtttga
     1921 gaagactgct cgatggtttg agcgggtgag tgcctctctt ctcgagagcc ttccttgcac
     1981 ccagggctgt tccgattgtt gtgtcggcct gtttcccgtg actatcctgg accggtggga
     2041 aattcaacgc ggtctccgaa gtctggctga tgagcatcgg aagagaatcg aacggacggc
     2101 agcggagcaa atcgctgtgc taatgatcgc cgcaccgcaa ctgaggacga atcattttat
     2161 cgaccagtgg acggagaagg acattgatcg agtcagcgaa caattccaga ggtggccctg
     2221 tccggcgttg gaggtggatg gaacgtgtgg actctatgag tttcggccgc tggcctgtcg
     2281 ttcaatgggc gttccccagg atgacggagt cctggtcaat ggcgcttgtg ctgtgcaaac
     2341 agccgtgcca ctgattcggc cctcaaagtc ttccgggagg aggaggatca tcttgctggg
     2401 atggaagcgg tggaaatcga ggccttgcgc ctccaagcag gaatcatggg tgaagagctg
     2461 ttcctgccct atgctttcat ccgggatccc gacgtgggag agctggagtt tggattggga
     2521 cagcggcgaa ttcccaactg acggtaatat ggtacgcgct agacagctta tttctcgcta
     2581 tgctaaggtg acgttccttg gttgcgggag cgcctgtagc tcagctggat agagcatcag
     2641 cctccggagc tgagggccac aggttcaaat cctgtcaggc gcaccaaaaa cacataccaa
     2701 caccaggttg aggttgagga agcccacgag ccggttccct ttcttcaacc tgaacctcaa
     2761 gctttcttga acccggtggg gccgttagct cagttggtag agcagctgac tcttaatcag
     2821 cgggccgtag gttcgacccc tacacggccc accaaacttt tcaatcactt acacgcctga
     2881 cacccgctga gacctggccg gggtggacta attctgtacc ctggcccacg agccgatcca
     2941 acaggtcgat ccaatcgcgc aggctttccg gatagtgatg cgcatagcgt tgcgtcatga
     3001 cgggccgatt tatgccccag cagatggagc gctcggagtg cgcgaacagt tcctgtgtca
     3061 gatcaatatg gcctctcagc tcactaccct cttccaagtc gcttcgtacg tcttcaactg
     3121 cgagtcgcat gaggctggca tctactttcg atcggtgttc cgcagccatg aaaagcagat
     3181 ccaatcgttt cttgcactgg ttgaggatat gcattgccag cgaagcatct cctgcaaacc
     3241 attcagccag aaatttcgtg atgtgtcaca tcactgatct cgccaaggca tcgagtttga
     3301 ccttgatccg cttcggcacg ccgatcagca gcttcaccat cgccatccca tcacctccaa
     3361 tggttataag tttatattta tatattatta actgtcaatt catattattg actatatcct
     3421 ctgtcgccag tacagtagct ggaacatggc cactaagggc actaacccgc cggaaaagcg
     3481 gagaaaactt ttcggcttga gtctggatcg acaactcatg ctcgaggttc aacaccttgc
     3541 actcgaccaa gaccgttatg tgaacgagat gatcgaagaa gccgcgaagg atttactgaa
     3601 gaagtaccgg gagaaggcga aatagatgac cttcagctta tgggctgatg aggttgtgag
     3661 ggaaagaccc ttgaagattg agcctaatgc ctcctgcatt cgggcgatga atgctcgccg
     3721 gtgagggcgc atggacattc tcattctctg tctcatggtg ttgatgggtt ggtctgcttg
     3781 gattgccttt cgcccctcat
//
LOCUS       DATNDD010000010         9779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_284293, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000010 DATNDD010000000
VERSION     DATNDD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284293"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VN647_00220"
     CDS             <1..242
                     /locus_tag="VN647_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWG95476.1"
                     /translation="WLAQTPQAFRRDWLLAAHRKAHAEGIRATDDAYLIEWVGHPVCV
                     VEGTGENIKVTRPEDMVIGEAILASRQSRGMKGSV"
     gene            239..724
                     /gene="ispF"
                     /locus_tag="VN647_00225"
     CDS             239..724
                     /gene="ispF"
                     /locus_tag="VN647_00225"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWG95477.1"
                     /translation="MKKGSRIGYGYDVHPLGADRSLILGGIEIPHIKGLLGHSDSDVL
                     VHAVCDALLGAMGEGDLGRHYPSSDPKYKGISSLKLLEDVMAKLKVKGYRVGNIDSVI
                     VAQAPRLGPHLPAMQKKIAETAGIDPDLVNVKVKSGEALDAVGHEEGMIAHAVCLIEP
                     V"
     gene            744..1394
                     /gene="cysE"
                     /locus_tag="VN647_00230"
     CDS             744..1394
                     /gene="cysE"
                     /locus_tag="VN647_00230"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HWG95478.1"
                     /translation="MLTKIKQDLQAVFDRDPAATSKLEVILTYAGFHALLAYRITHWL
                     KARDIPILPRVISQLARWVTGVEIHPSAKIGTGFFIDHGMGVVIGETAEIGDYVTLFQ
                     GVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGITIGDNVKIGANSVVLKNVAANSTV
                     IGVPARVIKTQGTRLPDATMDQVDLPDPISDRLIALEQELIELRKRLENPDEQSHS"
     gene            complement(1448..1768)
                     /locus_tag="VN647_00235"
     CDS             complement(1448..1768)
                     /locus_tag="VN647_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HWG95479.1"
                     /translation="MATSLRALTLGLFVLSLGVTPFAVAAPTQQNKMKACNDQANEKA
                     FGEGKGEERKAFMKDCLSAKPEKNDGGKENQQNKMKTCNKEAGEKKLKGDERKKFMSD
                     CLSN"
     gene            complement(1864..2601)
                     /gene="truA"
                     /locus_tag="VN647_00240"
     CDS             complement(1864..2601)
                     /gene="truA"
                     /locus_tag="VN647_00240"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWG95480.1"
                     /translation="MPIVKLILEYDGTAYAGWQRQPDQPTIQEAVETAILGVTQITAS
                     VIGAGRTDAGVHALGQVASFRIEHDMTSREWTRALNAHLPKNIVVRSTTLMPDIFHAR
                     HSAKGKLYEYRILNRSERPAVARDYCWHIHQPLDDAAMNQTGLALIGPHDFSSFQTQP
                     TDNDDPMCHLQRFTVFREGDRLRIEAYADRFLKQMIRSIVGTLVEVGLGKRTPESIKP
                     ILQAHDRSAAGKTAPPQGLFLVRVDYD"
     gene            complement(2632..3915)
                     /locus_tag="VN647_00245"
     CDS             complement(2632..3915)
                     /locus_tag="VN647_00245"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWG95481.1"
                     /translation="MQVRKPRLLSIKPLALLSCSVIFLLSASGILIQAAWAWAPDDRH
                     PLPVMSSKHNRARGTTASLAPVTVRNFRVMTSPERTRLVLDLDRHTTVIEQRASNPSR
                     VVIALPNAWLGRPARIKARNGTIPSPFMIIQLPHQAVAVSLPTTSFRTYKHFTLSHPP
                     RLVIDVTPSDAQVRPDAPDPQEIPQRSISPVSQPVIPRAKGYTTIVIDPGHGGKDPGA
                     RGIQKTEEKDITLKVGLQLRELLRKQSGLRVLMTRDHDVFIELEDRAKFANSNEADLF
                     VSIHVNSHPSRAVKGIEIYHFGEAKDQRALEVAARENGTPISNTGVGWEYLVADLLTT
                     KKIEASLDLAWNAKEAMVSRMNGQYVLNDHGVKTAPFYVLRFTSMPSILAEIAYISNP
                     DEEDLLRKPVFVQDVAQSLYHGIVSFLANNRSDVR"
     gene            4042..4563
                     /locus_tag="VN647_00250"
     CDS             4042..4563
                     /locus_tag="VN647_00250"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWG95482.1"
                     /translation="MSTHLIVDGYNLLAGTGSLSGHLESAREALVQDLAAYRHRKNHL
                     IIVVFDGWQQGRPSEQREHRAGVQVIYSKRGERADQVIQRLAREYGSDCAVVSSDHEI
                     VNAAKAHGAFIMGAREFAGKLRVSSGAVGTIPYKELDSGDDIHPRRGPEKRGNPRKLP
                     KAQRQRDRRLKRF"
     gene            complement(4550..5419)
                     /locus_tag="VN647_00255"
     CDS             complement(4550..5419)
                     /locus_tag="VN647_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWG95483.1"
                     /translation="MPNRVRRSLDSIGAIIHHPIDLPLDSALMLRSPTMLIDTHTHLD
                     DARYNEDRDAVITRARDAGVGAFLTIGCDLTTSEAAVVLAERYPFVYASIGVHPHEVR
                     HIQDNWYDTFRRLAGNNKVVAYGEIGLDYHYNHSSPKEQRDRFREQIQVARELKLPVI
                     IHTREAQDDTVTILKTERASEIGGVFHCFSGDSRLAKEALDLGFYLSFSGILTFQNAA
                     PLREIAKYTPLDRVLIETDCPYLTPVPYRGKRNEPAYVSQVAKQLASLYNRSPDEIAG
                     RTSENARRLFKIA"
     gene            5708..6133
                     /gene="exbB"
                     /locus_tag="VN647_00260"
     CDS             5708..6133
                     /gene="exbB"
                     /locus_tag="VN647_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HWG95484.1"
                     /translation="MNVLIHLVDYGIIGLLVVLSLWALAVAVERWFYYRRVTPNQFEN
                     IQTMEIALTKRLVVIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVGAIMVGLSL
                     ALKATAIGLLVAIPCVVLNNILRRRVTELLTLYKVQHGA"
     gene            6123..6497
                     /locus_tag="VN647_00265"
     CDS             6123..6497
                     /locus_tag="VN647_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWG95485.1"
                     /translation="MERELNQINVIPLVDVMLVLLVIVLTTATFISTGQVPVELAKAK
                     EGNDHRDAPLMITLTADGRLFVNDQPIQADGLATVLNEHSRESAVILRADRVTVLERF
                     IGVVDEVKGLGFRQVSLEVLRS"
     gene            6494..7351
                     /locus_tag="VN647_00270"
     CDS             6494..7351
                     /locus_tag="VN647_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWG95486.1"
                     /translation="MTTGELNRISVGTVPEQARGWMASVLLHLLGLAGAMLIMAKIEQ
                     SVLLEPFHWDISMVDAPVRTEPTQSESMPAERVIQPPPSPVKPKPPVRTATQPIAQQT
                     PLPTHDESTVPVETTQIVKDVVTNAEPVMESPVVESVESRPVTSQAVMAEPVASSEAT
                     TIEQRIESTETAVEQRVVQQRLVHYRRTQADYGWLGDMVRKRIEELKHYPRIARENHW
                     EGRVVVQAVIKADGTVGELSVAESSGHALLDEEALAVMRKASPLTLKHQLEKSQITIL
                     VPISYRLEG"
     gene            7405..7863
                     /locus_tag="VN647_00275"
     CDS             7405..7863
                     /locus_tag="VN647_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95487.1"
                     /translation="MNRRTIIRSIIRTASVISVMGTLCLFGDPAWAQSKNEKAFHAVA
                     VDAKNVETDVKNIIFYYEEKISETAFVPHELRELPVKRGTATVKIKFDGIKHIDLKPS
                     GNGTAPNVTITLNDGKTGEFILAVAGNFKGQSEFGEVELPAAELKKLAFK"
     gene            8495..9724
                     /locus_tag="VN647_00280"
     CDS             8495..9724
                     /locus_tag="VN647_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWG95488.1"
                     /translation="MITDRLTTVLPQPDMKGAVDSPEALCPPIRAQRRFKLRKFWLKL
                     HLYLGLFGGGLFVLTSLSGSFLVFYKAIDEWLNPALVVTSGKDTYRPLNEIVTAAKAA
                     SPVGAVLDSVEWPSHQHGVFLTWHKMQTDHADEFRWIQVTIDPSTAAVLTKDREWGGY
                     LVSLIYNLHASLLMDDFGATIVGFVAIFLLVSIGIGMYLWWPRPARLRQAFTATPGGS
                     VIRRQYEWHRLSGFYGAPILGMLAFTGTYLEFGDYVIPIVRFFSPIQEFSEEMTMQSL
                     PTPDARPLSIEQVVSLAQQVFPTGELRYIGVPHEDTDVYHLALRQTGEVGESNGQSQV
                     WLDQYSGKVLRLHDWNRFTGGETFLAWLFPLHNGEAFGLTGRWIVFIAGFVPLLLYIT
                     ALRMWWLKRQTHRRQLV"
BASE COUNT         2162 a         2478 c         2876 g         2263 t
ORIGIN      
        1 tgtggttggc tcaaaccccg caagccttcc gtcgcgattg gttgttggca gcccatagga
       61 aggcccacgc agaagggatt cgtgcaaccg atgatgctta tctcatcgag tgggtcggac
      121 atccagtgtg tgtcgtcgaa ggaaccggcg aaaacatcaa ggtcacgagg ccggaagaca
      181 tggtgatcgg cgaagcgatc ttggcatcgc ggcaatctcg cggcatgaaa ggatcagtat
      241 gaagaagggg tctcgcatcg gctatggcta cgatgttcac cccctcgggg cggaccgttc
      301 attgatcttg ggagggattg agattccaca cataaaagga ttgctcggtc attctgattc
      361 cgatgtcctt gttcatgccg tatgcgatgc tttattgggg gcgatggggg aaggggatct
      421 cggcaggcac tatccaagtt ccgaccccaa gtataagggg atttccagct tgaaactgct
      481 ggaagacgtt atggcgaagc tgaaagtgaa ggggtatcgg gtgggaaaca tcgactccgt
      541 gattgtggct caggcccctc ggcttggccc gcatcttccg gcgatgcaga agaaaatagc
      601 ggagaccgcc ggcatcgatc cggatctggt caatgtaaag gtaaaaagcg gggaagcctt
      661 ggatgcggtc ggacacgaag aggggatgat cgctcacgcc gtctgtttga tcgaaccggt
      721 ttgacgtcgt aagagaccac gtgatattga cgaaaattaa acaagacctt caagccgtct
      781 ttgaccgcga tccggccgcg accagcaagt tggaggtgat cctcacctac gccgggttcc
      841 atgcgcttct ggcgtatcgc atcactcact ggctgaaggc gcgcgatatc ccgatcctgc
      901 cgagggtcat ttcgcagttg gcccgctggg tgaccggtgt ggagatccat ccttccgcaa
      961 aaatcgggac cggctttttc atcgaccacg ggatgggagt tgtaatcgga gaaacggcgg
     1021 aaatcgggga ttacgtgacc ctctttcaag gggtgacctt gggtggcacc ggcaaggagc
     1081 ggggcaaacg gcatccgacg ctcggtaatc atgtggtggt gggcgcaggt gccaagatct
     1141 taggagggat tacaatcggg gacaacgtga agatcggggc gaattccgtg gtactcaaaa
     1201 acgtcgcggc taattccact gtaataggag tgcctgctcg ggtgatcaag actcagggga
     1261 cgcgtttacc ggatgcgacc atggatcagg tcgatctgcc cgacccgatc agtgatcggc
     1321 tgatcgccct tgaacaagaa ttaatcgaac tccgcaaaag actcgaaaat cccgatgagc
     1381 aatcccattc gtaacagtca tcgatacagg caggcacgtg cacgaatcag actggcgcgg
     1441 ccatcggcta atttgagaga cagtcgctca tgaatttctt tcgctcgtca cccttcaact
     1501 tcttctcgcc cgcctctttg ttgcacgtct tcatcttatt ctgctgattc tccttgccgc
     1561 cgtcgttctt ctcaggcttg gcggagagac agtccttcat aaacgccttc cgttcctcgc
     1621 ctttcccttc gccgaacgcc ttctcattcg cctggtcgtt gcacgccttc atcttatttt
     1681 gctgtgtagg cgcggccacg gcgaacgggg tcactccaag actgagcaca aacaacccca
     1741 gtgtgagagc acgcaaggat gtcgccatgc tggatctcct tctgtagtga gggatgagct
     1801 gcaagtctgg atcgcatcat agcaaagcga tacgtaattg tctgtcaatg aatggggtag
     1861 gggtcagtcg tagtcgaccc gtaccaaaaa gagtccttgt gggggagccg ttttaccggc
     1921 ggccgaacgg tcatgtgcct ggaggatggg cttgatgctc tccggcgtgc gtttgcccaa
     1981 gccgacctcc acgagcgtcc cgacgatcga gcggatcatc tgtttcagga accggtcggc
     2041 ataggcttcg atccgcagcc gatcaccttc acggaagacg gtgaatcgct gtagatgaca
     2101 cataggatcg tcgttgtcgg tgggttgggt ctggaaggaa gagaaatcgt gcggaccgat
     2161 cagggcaagt cctgtttgat tcatcgccgc gtcgtcaaga ggctggtgga tatgccagca
     2221 gtagtcacgc gcaaccgccg gacgctccga gcgattcaga atgcggtatt catacagttt
     2281 gcccttggcc gaatgtctcg cgtggaatat gtccggcatg agtgtggttg atcgcactac
     2341 gatgtttttc gggagatggg cgttcagcgc cctggtccat tctcgggacg tcatgtcatg
     2401 ctcgatacgg aagctcgcga cctgccccaa tgcgtgaaca cccgcatcag tgcgtccggc
     2461 gccgatgacc gatgcggtga tttgagtgac gcctaggata gctgtttcga cagcttcctg
     2521 aatcgtgggt tggtcgggct gacgttgcca gcctgcgtag gccgtgccgt catattcgag
     2581 aatgagtttg acgatgggca tgaagcacgt ggatggtgat gagtaaaacg atcatcggac
     2641 gtccgatcga ttgttggcaa gaaacgaaac aataccatgg tagagggatt gagcgacatc
     2701 ctgaacgaac accggtttcc tgaggagatc ttcttcgtcg gggttggaaa tataggcgat
     2761 ctcggccaga atgctgggca tactcgtaaa cctcaataca taaaagggcg ccgtcttgac
     2821 gccatggtcg ttgagtacgt attgcccgtt cattcgggaa accatggctt ctttcgcgtt
     2881 ccaagcgaga tcaagggaag cctcgatttt cttcgtcgtt aagagatccg ccacgaggta
     2941 ttcccatccg actccggtgt tgctgatggg ggtgccgttc tcccgtgctg cgacctcgag
     3001 agcccgctga tccttagctt ctccgaagtg atagatttca atccctttga cagcacgcga
     3061 tggatgagag ttgacatgga tggagacaaa gagatcggct tcgttgctgt tcgcgaattt
     3121 ggctcgatct tccagctcaa tgaacacatc atggtcccgg gtcatcaata cccgcaggcc
     3181 ggactgcttc ctcaggagct cgcgaagctg gagtcccacc ttgagggtaa tatccttctc
     3241 ctcggtcttt tggatccccc gtgcgcccgg gtctttcccc ccatggccgg gatcgatgac
     3301 gatggtcgtg tatcccttgg cgcgggggat cacgggttgc gagacaggag aaattgaccg
     3361 ctgagggatt tcttgcggat caggtgcgtc aggacggact tgtgcatcgg atggagtcac
     3421 gtcaatgacc aagcgaggcg gatgggaaag cgtgaagtgc ttataggttc ggaacgatgt
     3481 ggtcggaaga gacacagcaa cagcctggtg gggcaattga atgatcatga agggtgaggg
     3541 gatggtccca ttcctggctt taattcgtgc cggccggccg agccatgcgt tgggaagcgc
     3601 gatcactact cggcttggat tggaagctcg ctgctcgatg acagtggtgt gacgatcgag
     3661 atctaaaacc agtctggttc tctctgggct cgtcatcacg cggaaattac gaacggtaac
     3721 gggagcaagg gaagcagtcg ttcctctggc acggttgtgt ttcgatgaca tcaccggcag
     3781 aggatggcga tcatcggggg cccaagccca ggccgcttgg atcagtatcc cggatgcgga
     3841 caggaggaag atgacactgc acgagagaag agcgaggggc ttgattgaaa gcagccgagg
     3901 ctttcgtact tgcatgggcc ctttggggta gaggacaatg agaaactatt ctcagatgtc
     3961 cgccgccttg tcaagaatta caccagttgg ctatgcggaa ttcggattga ctgtctgctc
     4021 atgaccacga tagcgtgagg tatgtcgacg catctgatcg tcgatgggta taacttgctg
     4081 gctggtaccg gaagcctttc cgggcatctt gaatcggccc gcgaagcatt ggtgcaagat
     4141 ctagcagctt atcgacatcg aaagaatcac ctcatcatcg tggtattcga cggctggcaa
     4201 cagggccggc catcagaaca gcgggagcat cgtgccggag tccaggtgat ctattcaaaa
     4261 cggggtgaac gggcggatca agtcatccaa cggttggcac gggagtatgg ctcggactgt
     4321 gcggtcgtca gttccgatca tgagatcgtg aatgcggcta aagcccatgg ggcttttatc
     4381 atgggtgctc gtgagtttgc gggtaagctc cgtgtgtcat cgggtgcggt cggcaccatc
     4441 ccatataaag aactggattc tggggacgat attcacccaa ggcgcggacc ggagaagagg
     4501 ggaaatcctc gcaagcttcc aaaggcgcaa aggcaacgag accgacgact taagcgattt
     4561 tgaacagcct cctggcgttt tcgctggtcc gtccggcgat ttcgtcaggt gatctattgt
     4621 aaagcgacgc caactgtttt gcgacctgcg acacataggc cggttcgttg cgttttcctc
     4681 ggtagggaac gggagtgaga taggggcagt cggtttcgat cagcacacga tcgagggggg
     4741 tatatttagc gatctctctc aacggtgccg cattctgaaa cgtcaggatt cccgaaaacg
     4801 acaggtagaa cccaagatcc aaagcctctt tagccagccg gctatctccg gaaaaacagt
     4861 gaaacacccc accaatctct gacgctctct ccgttttcag gattgtaacc gtatcatcct
     4921 gtgcttccct cgtgtgaatg attacgggga gctttagttc ccgtgcaacc tgaatctgct
     4981 cacggaatcg gtcgcgctgc tctttcggcg aagagtgatt gtaatggtag tccagcccga
     5041 tttccccata ggccacaacc ttgttgttcc ctgccagacg acggaaggta tcgtaccagt
     5101 tgtcttggat atgccttact tcgtgcgggt ggacgccgat ggaggcatag acaaatgggt
     5161 atcgttctgc gagtaccact gctgcttcac tggtggtcag atcacaaccg atggtgagaa
     5221 atgctccgac tcctgcatct cgcgcacggg tgatgacagc atctctgtct tcgttgtaac
     5281 gggcatcatc caaatgggta tgagtgtcga tcaacatcgt tggggatcgt agcatgagtg
     5341 ctgagtcaag aggaaggtcg atcgggtggt gaataatggc gccgatcgaa tcaagggagc
     5401 ggcggactct gttcggcatt tataacggac tcgccatatg gagtcggttt tcgaacgatg
     5461 agtttttagg attgacaaaa cataacaggg tagattattg atactagtta tcataatcat
     5521 gtcacgaggc caattgtgac ataggtcctc ggttagaggc cagagtactt tacgtccaga
     5581 gcccatggct gcctaaaggc ggccatgggc ttttttatgg acttggcagg gccaaaggca
     5641 ctgagagctc ctaagcggca ttaagaagaa cattctggtc attctgtaaa cagaggatct
     5701 tttctacatg aacgtactta tacacctggt ggattacgga attatcgggc tgctggttgt
     5761 gctcagccta tgggcattgg ccgtggcggt ggaacggtgg ttctactatc gacgtgtgac
     5821 gcccaatcag ttcgagaaca ttcagactat ggaaatagcc ctgacaaaac gactggtggt
     5881 aatcggaacg gtggcagcca acgcgccgta tatcgggctc ttgggaactg tcttgggcat
     5941 catgttgacg ttccatacga tgggcacctc ggggacgatg gcggtcggtg cgattatggt
     6001 cggattgagt ttggcgctca aggcgacggc tattgggttg ctggtggcga tcccatgcgt
     6061 ggtgctcaac aatatccttc ggcggcgcgt caccgagctg ctcacgctgt acaaggtgca
     6121 gcatggagcg tgaactgaat caaatcaatg tcattccact ggtggatgtg atgctggtgc
     6181 tcttggtcat cgttttgacc acggccacgt tcatatcgac ggggcaagtg ccggtcgaat
     6241 tggcgaaggc caaggagggg aacgaccatc gagacgcgcc gttgatgatt actctgactg
     6301 ccgatggacg cctgtttgtg aatgatcaac ctattcaggc ggatgggttg gcgactgtcc
     6361 tgaacgagca ttctcgggag tcagcggtca tattgcgagc tgatcgcgtt acggttttgg
     6421 agcgatttat cggcgtggtc gatgaagtaa agggactggg atttcgacag gtcagtttgg
     6481 aggttctacg atcatgacca ccggcgaatt gaatcgcatc agtgtgggca ctgttcctga
     6541 acaagcacgt ggttggatgg cctctgtatt gctgcatctc ctgggcctcg ccggcgcgat
     6601 gctcatcatg gcgaaaattg agcaatcggt cttgctagaa ccatttcatt gggatatctc
     6661 aatggtagat gctccagtcc gcactgaacc gacccagtct gagtccatgc cggctgaacg
     6721 agtgatccag ccgccccctt cacccgttaa accgaagccg cctgttcgaa cagccactca
     6781 gccgatcgcc caacaaaccc cgctaccgac tcacgatgag tcaacggttc ctgttgaaac
     6841 gacgcagatc gtgaaggacg tcgtcacaaa cgcagaaccg gtgatggagt ctcccgtcgt
     6901 ggaatcggtg gaaagccgac cggtgacttc tcaggctgtc atggcagaac cggttgcctc
     6961 atccgaggcg acgaccatcg aacagcgcat tgaatctacg gaaacggcgg tggaacagcg
     7021 cgttgtccaa cagaggctcg tgcactatcg tagaactcag gcggactacg gctggcttgg
     7081 ggacatggtc aggaaacgca ttgaagaatt aaaacattat ccaaggattg cacgtgagaa
     7141 ccattgggag ggtcgagtcg tcgtacaggc ggtcatcaaa gcggacggca cggtcggaga
     7201 gctcagtgtc gctgaaagct caggccatgc attgctggat gaagaggcgt tggcggtcat
     7261 gcgaaaagcc tccccgttga cgctcaaaca tcaattggag aagtcgcaga ttaccattct
     7321 ggttccgatc agttatcgtt tggaagggta acaagcggag caagaaactc tagtcgagtg
     7381 gaacacgaac aaaggagaat cacgatgaat cgtcggacga tcatacgctc aatcattcgc
     7441 accgcgtcgg tcatctctgt aatggggacg ctttgtctat tcggcgatcc tgcctgggcc
     7501 cagagcaaga acgagaaggc cttccacgcc gtggcggtcg atgccaagaa tgttgaaacc
     7561 gacgtcaaga acatcatctt ttattatgaa gagaagatca gtgaaacggc gttcgtcccc
     7621 cacgagcttc gtgaattgcc ggtaaagcga gggactgcca cagtcaagat caagtttgac
     7681 ggcatcaaac atatcgacct caagccatcc ggtaacggca cagcgccgaa tgtgaccatt
     7741 acgctcaacg atggcaagac aggggaattt atcttggccg tcgcaggcaa tttcaaaggg
     7801 caatccgaat tcggagaagt cgaattgccg gctgctgagc tgaaaaaact ggcatttaag
     7861 tagcgttgct tcactgcggt cggcaattgc tcaaggagga tccattctga atctgctccg
     7921 agtcggctta aagggagaac gcatgtcttt ttattttcat cgagagatct ccttgccata
     7981 tcgaaccctc ggcaggttcg tgaacactca tgcgccatat cccccgtgga tgggattcaa
     8041 tcgttctcca tgtggccgac gaggcatacc gtgcgcgagc aaggtagaac catgagtgag
     8101 tctcgaacgc ctgccggatc gggtgtagat gtccggtgcc actgcgggaa gctgatggcc
     8161 cgctggcaag gccagagctt ggtcatcaaa tgtgcgcgct gtaggcgatt cattaggatt
     8221 cacgcgtccg ccatcggcgg taggtcacct gaactgtaac ttggtttaca tcggtagctt
     8281 ctgttgtagt cagtccacca cctaggctgt gattccttga agtcagcatg tactgcctgg
     8341 aggggcggcg aggatagccc tgcgagcccg tgcatctgag gtccgctgaa caaagagccg
     8401 ggtgagcttt ctgcatgctt tcccgtttta agtgaattgg tgaggctgtt cacacacagg
     8461 ccacgttcct cgctagatcc agtggacgac cgtcatgatt accgaccgac tgactactgt
     8521 ccttccacag cctgacatga agggcgccgt cgattctcct gaagccctat gtcctcccat
     8581 acgagcgcag cgtcgtttca agttgagaaa attctggctc aagctccatc tttatctcgg
     8641 actgttcgga ggcgggttat ttgtattgac gagcctcagc ggcagcttcc tcgtcttcta
     8701 taaagctatc gatgagtggt tgaatccggc gttggtggtg acgagcggca aggacaccta
     8761 ccggccgttg aatgaaattg tcacagcggc caaagcggct agtcctgtag gcgccgtgct
     8821 ggacagcgtc gaatggccgt cgcaccagca cggtgttttt ctcacctggc acaagatgca
     8881 gaccgaccac gccgatgagt tccggtggat tcaagtgacc atcgacccgt ctacggcggc
     8941 cgtgctgacc aaggaccgcg aatggggtgg gtacttggtt tcgttgattt acaatctgca
     9001 tgcatcgctc ctgatggacg attttggagc gacgatcgta gggtttgttg ccatttttct
     9061 gctcgtctcg atcggcatcg gtatgtatct ctggtggcca cgtccggcca ggctccgtca
     9121 ggcattcacg gccacgccag gtggaagtgt catccgccgc cagtacgaat ggcatcggct
     9181 cagcggcttc tacggcgcac cgatcctggg gatgcttgcc ttcacaggca cctacctgga
     9241 attcggagac tatgtcatcc caatcgtccg attcttttcc ccaatccagg aattctccga
     9301 ggaaatgacc atgcagtcgc ttccgactcc tgatgctcgg ccattgtcca ttgagcaagt
     9361 tgtctccctt gctcagcaag tcttcccgac gggggagctg cggtacatcg gcgtaccgca
     9421 cgaggacacc gacgtctatc atctcgctct gcgtcagacg ggtgaggtgg gcgagtcgaa
     9481 tggtcaaagc caggtgtggt tggatcaata cagcggcaaa gtgcttcgcc ttcacgattg
     9541 gaatcggttc accggcggtg aaacctttct ggcctggctc ttccccctgc ataacggtga
     9601 agcgttcggc ctcactggac ggtggatcgt tttcatcgcc ggatttgttc cgctcctgct
     9661 gtacatcacc gcgctgcgca tgtggtggct gaaacggcag actcaccgga gacagctggt
     9721 ctgacctcca tcaggttctg atagaggctg gactttgacg ttattctcgg tcgctgagg
//
LOCUS       DATNDD010000011         5700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4008417, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000011 DATNDD010000000
VERSION     DATNDD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4008417"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN647_00285"
     CDS             complement(<1..359)
                     /locus_tag="VN647_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWG95489.1"
                     /translation="MNEWGPSLAVILGIVEGLTEFLPVSSTGHLILVGHALGFTGDVA
                     ANAEISIQLGAILAVIVFERKKIGRLLSGAWQEQQVLCSASANEPSAAWTDRVKVSMR
                     SHPNLWFLLGLGVAFLPA"
     gene            682..1368
                     /locus_tag="VN647_00290"
     CDS             682..1368
                     /locus_tag="VN647_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95490.1"
                     /translation="MRREWTAIVLVGAVMTTGCVSNKKYQEALAEADNVKMELDKTRQ
                     QKSAMEQQVRTLKEVNAKFGNEAQAARDELQRIEHGRDAERSAIEGRTKELEDKVKAL
                     SAQNKNVRQEYQDVKRHNDTLKSLVARYQKELKDRSNAGGPPQTATVTPNQALPGTAT
                     APQAAASAAMNINKVSANDMILFLGLKQDEADRIVSNRPYRVKGELVAKNVVPKETFD
                     LIKDRISVSP"
     gene            complement(1507..4995)
                     /locus_tag="VN647_00295"
     CDS             complement(1507..4995)
                     /locus_tag="VN647_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HWG95491.1"
                     /translation="MTTSAGSETQPLSHPLRGLLIAQFCGAFNDNAWKLMVALLAIRQ
                     ATAGMASGPALETVVQTQTAMAFVIFTLPLVFLSLVGGTLADRVSKRTVIIAIKIVEV
                     LLMAAGTVALWLNPAGGVLPLIVLCGMGVHSALFSPSKYGILPELIPHEQLAAGNGLL
                     EMWTFAAILTGTAAGGFLLQIAGAQTWLAPMALTGLSIIGLVTAFSIPQVSPARAAGG
                     VGTTLQGAWEAIQSERMLRMAIPMEVMFWTIASLFGQNVLVYAKAVLHLSDAMSGLPL
                     TILSVGIGIGAMLVGRISRNRVEYGLIPVGAIGVFLTLLSLGFLAPPLTETFFMMGVL
                     GIASSFIFVPLNAILQWKSPSDRRGAVISFSNTCVFTGILLGSLVGGSLANAGLSTTG
                     IFLAAAAMTLGGIGWALWFLPDTFIRLVLVILTNTLYRLRIVGQSHVPPSGGALLVPN
                     HVSFIDGFLLIASLDRPVRFVVDSQYAEQPIFKPFMKTLGVIPISSHGGLRVILKALR
                     DAGAAVDKGELVCIFPEGQITRTGTLLPFRRGFERVVKGRTAPIIPVHLDRVWGSIFS
                     FNHGRFLWKVPEQLPYPVTVSFGTPLPPGTPAHELRGKIQELGEAAWQFRKPSRQPLH
                     RQFIRSMRRYPFRLAMADQNRPRVSSLQALIGSIVLARTLRPYWKDQQRVGVLLPPTV
                     AAALVNVAAPLCGKTIVNLNYTVGKSGLEAAVEQADLRTIVTSRVFIEKAKLELPDGP
                     SVVWLEDVAATISNGQKVLAALLALFVPCRFIERACGQTIPLTPDSMTTIIFSSGSTG
                     EPKGVMLSHFNIDANSQGATQVLHFYQDERVLGILPFFHSFGYMVFWFVMSNNATIIF
                     HPSPLDVAAIGELIRRHQITFLVTTPTFLQLYSRRCTPEQFGSVRVILTGAEKLPTRL
                     AEAVEDKFGIGPIEGYGVTECSPVIAVNCPDFRAAGYYQPASRRGTVGQPLPGVSVQI
                     VDPDSYMPLPAGTPGMLLVKGPNVMNGYLGREDLTAQVMRGSWYITGDIAMLDDDGFL
                     TITDRLSRFSKIGGEMVPHGKVEEALQQAAGSEAQVFAVTGLPDEKRGERLAVIHTLD
                     EDRIPDILAKLSGSGLPNLFIPTRSQFVKVEALPVLGTGKLDLRGVKRMAMERLSSPE
                     TSELRSTEADQKSIDE"
     gene            complement(4992..>5700)
                     /locus_tag="VN647_00300"
     CDS             complement(4992..>5700)
                     /locus_tag="VN647_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HWG95492.1"
                     /translation="WPTPLVAAGERNGTPPAFHSDQLGHPLLPAKTRVANDVHLKGLG
                     SIHLITGSNMSGKSTFLRTIGINLCLAQAGGPVCARSFEWTWSRLACCIRVDDSLDAG
                     LSFFYAEVKRLKTILDAANDITAPPVLFLIDEIFKGTNNRERLIGSRAYITELSQRNG
                     FGLVSTHDLELADLEPAVPRLINAHFQETVSAGALEFDYRLRPGPCPTTNALRIMELE
                     GLPISPVPNIRQNAPHG"
BASE COUNT         1371 a         1514 c         1701 g         1114 t
ORIGIN      
        1 gcgggcagga aggcgacccc gagtcccagc aagaaccata agttgggatg actgcgcatg
       61 gagaccttga cgcggtcggt ccatgcggca gatggctcgt ttgcggaggc cgagcacaga
      121 acttgttgct cctgccaggc accggacaga agccgcccaa ttttcttccg ttcaaagaca
      181 atgacggcaa gtatcgcacc taactgaatg gaaatttccg cattggctgc gacgtcgccg
      241 gtgaagccga gggcatggcc gaccaggata agatgtcccg tggacgagac gggcaggaat
      301 tcagtaagtc cttcgacgat tcccagtatt actgcgagcg aggggcccca ttcattcatg
      361 catacaccat gttaggattt aagatgataa tgagggagag gtttcaacct tcacagccat
      421 ttctgataga atgggggcga tgatcacaga gcgacgacga ttcgtcaagc acggatgggt
      481 tgtcaggggg tagtcgcgtc agaacgggac cgattcccac aaaagcaaat aagttcccac
      541 aaaagcaaat aagttgacaa cgtccggatg ttggcttaca cacaaggaac agaatcggac
      601 gaaccacggt cgcagccgag tggatgtgat gttccacatg atacacaggc aaaccgtagg
      661 atttgatgga gggaggattg catgagacgc gagtggacgg cgatagtgtt ggttggggcg
      721 gtgatgacca cggggtgcgt atccaacaag aaatatcaag aggcgttggc cgaggcagac
      781 aacgtcaaga tggaactgga taagacccgc caacagaaga gcgccatgga gcaacaggtc
      841 cgaaccctca aagaagtgaa tgcgaagttc ggtaatgagg cacaagcggc tcgtgatgaa
      901 ctccagcgca ttgagcacgg acgtgatgcc gagcgaagcg cgatcgaagg acgaaccaag
      961 gaactcgagg ataaagtcaa ggcattatcg gcacaaaata agaacgtgcg gcaggaatac
     1021 caagacgtga aacgccataa cgacacgttg aaatcgctgg tcgcccgcta ccaaaaggag
     1081 ctcaaggatc gctccaatgc cggggggccg cctcaaacgg cgaccgtgac tccaaaccag
     1141 gctttaccag gaactgcgac ggctcctcag gcggcggctt cggcggcgat gaacatcaat
     1201 aaggtctcag cgaacgacat gatcctcttc ctcggactga agcaagatga ggcggatcgc
     1261 atcgtgagca atcgccccta tcgtgtgaag ggtgaattag tggcgaaaaa cgtggttcca
     1321 aaagaaacct tcgatctgat caaggatcgg atttcagtca gtccctaaag ctgaagtgag
     1381 cgccttttcg atatcagggc cgcccttccc gaaggggcgg ccctttttgt ttcttataca
     1441 aagataaacg tcaacttact cctatgcatc acttcacgat ccttcactgg tcatctttgg
     1501 tgaacatcat tcgtcaatcg atttctggtc cgcttccgta ctcctcagtt ccgatgtttc
     1561 aggtgatgag agccgttcca tcgccatgcg cttcacgcct cgtaaatcca gcttgccggt
     1621 cccgaggacc ggtaatgcct cgaccttgac gaactggctc cgcgtcggaa tgaagagatt
     1681 cgggaggccg gagccggaca attttgccag gatgtcagga atacggtcct catcaagggt
     1741 atgaatgacg gccagccgct ctcccctttt ctcgtccgga agaccggtca cggcaaacac
     1801 ctgggcctcg gatccggccg cttgttgcaa ggcctcttcg actttcccgt gcggaaccat
     1861 ttcgcctccg atcttcgaga atcgcgatag ccggtccgtg atcgtgagaa acccatcatc
     1921 gtccagcatg gcgatgtcgc cggtgatata ccagctacct cgcatgacct gagccgtgag
     1981 atcttcgcgt ccaagatagc cattcatcac attcggcccc ttcacgagca acattccagg
     2041 tgtgccggcc gggagtggca tgtaactatc aggatcgaca atctgcacgg acacaccggg
     2101 aagcggctga ccgacggtgc ctcggcgtga ggccggttgg tagtagccgg ctgcgcggaa
     2161 gtccggacag ttgacggcaa tgaccgggga acactccgtg actccatagc cttcgatcgg
     2221 tccgatccca aacttgtctt caacggcctc tgcaagtcgc gtcggcagtt tttccgcgcc
     2281 ggtgaggatc acccgcaccg aaccgaattg ttccggcgta cagcggcgag agtagagttg
     2341 cagaaacgtc ggcgtggtga cgagaaaagt gatctgatgt cgtcggatga gttcgccgat
     2401 cgccgccacg tcgagtgggg acgggtgaaa gatgatcgtt gcattattag acatcacaaa
     2461 ccagaacacc atgtatccga atgaatggaa gaacggcaaa atgccgagga cacgttcgtc
     2521 ctgatagaaa tggagcactt gtgtggcacc ctgactgttg gcatcgatat tgaagtgcga
     2581 aagcatgacg cctttaggtt ctcctgtgct gccgctgctg aagatgatcg tggtcatgct
     2641 gtcgggggtg agcggaatcg tctgcccgca ggcgcgttca atgaagcggc atggaacgaa
     2701 cagagccagc agggcagcca gcactttctg gccattgctg atcgtggcgg ccacgtcctc
     2761 cagccaaact accgacggtc catccggcaa ttcgagtttg gctttttcaa tgaagacacg
     2821 gcttgtcacg attgtgcgca gatctgcctg ttctactgca gcttctagcc cggatttccc
     2881 gaccgtatag ttcaagttta cgatggtctt cccgcagagg ggggcggcga cattcaccag
     2941 ggctgcggcg actgtgggtg gcaacaagac gcccactcgc tgttggtcct tccagtacgg
     3001 ccgaagcgtt ctcgcaagga cgattgatcc gatgagagct tgcaaggatg agacgcgggg
     3061 acggttctgg tcggccatgg ccagacggaa ggggtagcga cgcatggacc ggataaattg
     3121 gcgatggagt ggttggcgac tcggcttcct gaattgccag gctgcttcgc cgagttcttg
     3181 aatcttcccg cgcagttcgt gtgccggtgt ccccggcggt agcggtgtgc cgaacgagac
     3241 ggtcacggga tacggaagtt gttccggaac tttccacaga aagcggccgt gattgaagct
     3301 gaagatgctg ccccacacac ggtccagatg aaccgggata atgggagccg tccgtccttt
     3361 cacaacccgt tcgaaccccc gccggaacgg caacaacgtt ccggtacgag taatctgacc
     3421 ttccgggaag atgcagacga gttctccctt gtcgacggct gcaccggcat cgcggagcgc
     3481 tttcaaaatg acccgcaagc ctccatgaga ggaaatggga atgactccga gcgtcttcat
     3541 gaaaggtttg aagatcggct gttcggcata ttgcgaatca acgacaaagc gcacgggtcg
     3601 atccaagctg gcgatcagga gaaagccgtc gataaaggag acgtgattgg ggacgaggag
     3661 cgctccaccg gagggcggaa catgagactg gccgacgatg cggagccgat agagggtatt
     3721 ggtaagaatg accagcacca gccgaataaa cgtgtcaggt aaaaaccaaa gcgcccatcc
     3781 tatgccgccc agggtcattg cggctgcagc caagaaaatg ccggttgttg agaggcctgc
     3841 gttggccaag gaaccgccta ccagtgatcc caacaagatg ccggtgaaga cgcatgtgtt
     3901 ggagaacgaa atgacggcac ccctccgatc ggacggagat ttccattgga gaatggcgtt
     3961 caacggaaca aagatgaatg aactggcaat acccaacact cccatcatga agaacgtttc
     4021 tgtcaacggt ggggccagga agccgagcga cagtaaggtg agaaacacgc cgatggcgcc
     4081 aaccggaatc aggccgtact cgacccggtt ccttgagatt cgtccaacca gcattgcgcc
     4141 gattccgatg cctaccgaca ggatggtgag tggaagaccc gacatcgcat cagacagatg
     4201 caaaacggct ttggcataga cgagcacgtt ctgtccgaat aggctcgcaa tggtccagaa
     4261 catgacttcc atgggaatgg ccatacgcaa catccgttcg gattgaattg cctcccatgc
     4321 gccttgaagg gtggttccca cacctccggc cgcgcgggcg ggtgacacct gtgggatgct
     4381 aaaggcggtc acgaggccga tgatcgagag gcctgtcaat gccatcgggg ccagccaggt
     4441 ttgagcgcct gcgatttgta gaaggaatcc tcctgctgcg gttcccgtca gaattgccgc
     4501 aaaggtccac atctccaaga ggccgttgcc ggcagcaagc tgttcatgcg ggatcaattc
     4561 ggggaggatc ccgtacttcg aaggactaaa cagggcgcta tgcacaccca tgccgcataa
     4621 gacgatcaaa ggcaggacgc caccggctgg attcagccag agtgcaaccg tgccggccgc
     4681 catgaggagc acttcgacga tcttaatggc gatgataacc gtccgcttac tcacgcgatc
     4741 ggccaatgtg cctccgacga gagagaggaa caccaacggc agggtgaaaa tgacgaatgc
     4801 catcgcggtc tgcgtctgga cgactgtctc caatgcgggg cccgatgcca tacctgctgt
     4861 ggcttgccga atggcgagca aagcgaccat tagtttccac gcattatcgt tgaaggcgcc
     4921 gcagaattgg gcgatcaaga gaccccgaag gggatgtgac agaggttgag tttcagaacc
     4981 ggccgaggtg gtcatccgtg aggggcattc tgcctgatat tcggtacggg ggaaataggc
     5041 aggccttcca actccataat tctcagcgcg tttgtcgtgg ggcaaggacc tgggcggagc
     5101 cgataatcaa actcgagtgc accggctgac acggtttctt gaaagtgcgc attgatgagc
     5161 ctcggcacgg caggctccaa atccgccaac tccaaatcat gagtgctcac gaggccgaag
     5221 ccgtttcttt gcgacagttc ggtaatatag gcgcggctgc cgatgagccg ctcccggtta
     5281 ttggtgcctt tgaagatctc atcgatcagg aacagcacgg gtggtgcggt tatgtcgttg
     5341 gcggcatcga ggatcgtctt gagccgtttc acctcggcat agaagaacga caggccggca
     5401 tcgagcgaat cgtcgacgcg aatgcagcag gccagccggc tccacgtcca ttcaaacgag
     5461 cgtgcacaaa caggacctcc ggcttgtgca agacaaaggt tgataccgat cgttcgaagg
     5521 aaggtgcttt tgccggacat gttggagccg gtaatcaggt gaatggagcc gagtcccttc
     5581 agatgaacgt cgttggcaac acgggtctta gccggcagca acggatggcc cagttggtcg
     5641 ctgtggaaag caggaggtgt gccgttccgt tcgccggctg cgacgagggg agtcggccat
//
LOCUS       DATNDD010000012         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_558794, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000012 DATNDD010000000
VERSION     DATNDD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558794"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..297
                     /locus_tag="VN647_00305"
     CDS             103..297
                     /locus_tag="VN647_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HWG95493.1"
                     /translation="MNHITIPERREGPVDPMPKSPRPLEEGGGPRRPETESPEKDSLM
                     KRMRKVDPKQAERYRQRTGE"
     gene            400..1173
                     /locus_tag="VN647_00310"
     CDS             400..1173
                     /locus_tag="VN647_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HWG95494.1"
                     /translation="MGMQGDFYQLLKEQGYVLGSSGQAGPGQELTIATTILAFKYRDG
                     VLVAGDRRATAGNMVMYDRTDKVLEIDRYSVMAIAGVPATAYEMARILEHSFKYYRRT
                     QLQELSFEGKLRALSKLLKENVAAALAGTGAVAPIFAGYDAGQEAAKIYFYDILGAEF
                     EGVEYAVSGSGSSTIRGILHYLNTWGVQPLSAMPEEQATVQALRLLTSAAEFDSATGG
                     VNREAGLYPVIKFITADGVRMMPDLQLRSLFESGVSRSA"
     gene            1186..1995
                     /locus_tag="VN647_00315"
     CDS             1186..1995
                     /locus_tag="VN647_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95495.1"
                     /translation="MYEEPYRWVEAVGNRRQYLDDQLKEGTPVVGIAYERGILLMTMS
                     KGTPKLYEIYDRLALGGMGHPADLEKLRFNLLEMAHVEGFNRSASDVTGSRLMKYGIA
                     PVIKQAFEEVFKAPFIVKILLAEVGRKTGKDGFLTINYDGTFEEKKQYAVLAATSVVE
                     QKMMGYLRQQSIVSLKDAVDAAIRTWAVGDRAQRPAHEDDESHEEAKTSPDQQVIDAE
                     TLYAHLRRRIAEKTIECALLDRHVQGPSKYRALEADELRTMLPTDLSPSLT"
     gene            2000..>2701
                     /locus_tag="VN647_00320"
     CDS             2000..>2701
                     /locus_tag="VN647_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWG95496.1"
                     /translation="MLHRIFGLETEYGLLIHEDRPNQSPTWFAHKIRDHLFQVQRRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYLDMGHLEYASPECHSLTDAVASDRAGDFLLQQA
                     LEELGFSEVSLIKNNIDHETDATFGSHENYLVSRRFPFSRRGLAPFVTFLVTRQIFTG
                     SGRVGSAAPQDAWIQTDRLILPRSALGAQHVSESPFQISQRADYIVNDFFEWVQHNRA
                     IVNTRDEPLADPNQYR"
BASE COUNT          631 a          690 c          806 g          574 t
ORIGIN      
        1 ctttgatccg caggaagtcc gacgatgcag ggacgtcttc cagcgagccc attcaccggc
       61 tgatgcactg gcagcctgga gcaccagaaa ggaagcacag atatgaacca catcacaata
      121 ccggaacggc gagaaggacc agtcgatccc atgccgaagt cgccccgccc actcgaagaa
      181 ggcggcgggc cgcgtcgtcc ggagacagaa tcaccggaga aggatagtct catgaaacgg
      241 atgcgcaagg tcgatcccaa acaggcagag cggtatcggc agaggaccgg tgagtagtgg
      301 tgaagcagtg ctgagtgctg agcggagagc agactcagag tgatgctgtt ccggtcagac
      361 ccaggcacgc agcacttttc actcagcact gaggagcaga tgggtatgca aggcgatttc
      421 tatcaactct tgaaagaaca aggatacgtg cttggatcgt cgggacaggc cgggccaggg
      481 caggaactga cgattgccac aacaatcctt gcttttaagt atcgcgacgg cgtgctggtt
      541 gcgggcgatc ggcgcgcgac agccggcaat atggtgatgt acgaccgcac tgacaaggta
      601 ttggagatcg accggtatag tgtcatggcg atcgccggag tgccggcaac cgcatatgaa
      661 atggcgcgca tccttgaaca ttctttcaag tattatcggc ggacacaact gcaggaactc
      721 agctttgagg ggaaactccg agctctgtcc aaattgctga aggagaatgt cgcggcggcc
      781 ttagcgggga ctggagctgt agcaccgatc tttgccggtt acgatgccgg gcaagaggca
      841 gcgaagatct acttttacga tattctcggc gctgagttcg agggagtgga atatgcggta
      901 tccgggtcgg ggtcgtcgac cattcgtgga attcttcatt acttgaatac atggggtgtg
      961 caaccgctga gtgcgatgcc ggaagagcaa gcaaccgttc aagcgctgcg attgttgacg
     1021 agtgctgcag aattcgacag cgcgacaggt ggagtcaacc gcgaggccgg tctctatccg
     1081 gtcattaagt tcatcacggc cgacggtgtc aggatgatgc cggatcttca gctccgatcg
     1141 ctgtttgaat caggagtttc tagaagcgcg taaggatcat tcgctatgta tgaagagcca
     1201 tatcggtggg tggaagcagt cggaaatcga cggcaatatc tcgacgatca gcttaaggag
     1261 ggaactcctg tcgtcgggat cgcgtatgag cgtggcattc tgctcatgac aatgagcaaa
     1321 ggcacgccca agttatatga aatttacgat cgtttagcct tgggaggtat ggggcatccg
     1381 gccgacctgg agaagctgcg gttcaaccta ttggagatgg ctcacgtcga agggttcaat
     1441 cgctccgctt ccgatgtgac cgggagccgc ctgatgaagt acggcatcgc gcctgtcatt
     1501 aaacaagcgt tcgaagaggt gttcaaggca cctttcatcg tgaaaatctt gctggcggaa
     1561 gtaggccgga aaaccgggaa agacggtttc ctgacaatta actatgacgg gacttttgag
     1621 gaaaaaaaac agtatgcggt gcttgcggct acatcggtgg tggagcagaa gatgatgggg
     1681 tatctccgac aacagtccat tgtgtctttg aaggacgccg tggatgcggc gattcgtacg
     1741 tgggcggtcg gcgatcgagc gcaacggccg gcccacgagg atgacgaaag ccatgaggaa
     1801 gcaaagacca gccctgatca gcaggtgatt gacgcggaga ccctctacgc gcatctgcgt
     1861 cggcgcattg ccgaaaagac gatcgaatgc gccttgcttg accgccacgt gcaaggacct
     1921 tccaagtatc gagcacttga ggccgatgag ttgagaacaa tgcttccgac ggatctgagc
     1981 ccatcactca cctaatctca tgttacatcg tatctttggc ttggagactg aatacgggct
     2041 cttgattcac gaggaccgcc ctaatcagtc cccgacatgg tttgcccata agattcgtga
     2101 ccatctcttc caggttcagc ggcgaggcgt gctggacctt caccatcgcg ggcatgacga
     2161 gcctccaggc aatggagggt ttctcaccaa cgccggacga atttatttgg acatgggcca
     2221 tttagagtat gcctcgccgg aatgccattc cctcaccgat gcggtggcgt cggaccgtgc
     2281 cggagacttt ttgctgcagc aagccctcga agaactcggc ttttctgagg tgtccttgat
     2341 caagaacaac atcgatcatg aaaccgacgc gactttcggc tcgcatgaga actacctggt
     2401 ttctcggcgg tttccttttt cgaggcgcgg gctggctccg ttcgtgacgt ttcttgtgac
     2461 caggcagatt tttaccggct ctggccgtgt cggttccgcg gctccacaag acgcctggat
     2521 ccagacggat cgattaatcc ttccacggtc ggctctcggc gcgcagcatg tatcggagtc
     2581 gccctttcaa atctcccaac gtgccgacta tatcgtgaac gatttcttcg aatgggtgca
     2641 gcacaatcgg gcgattgtga acacgagaga cgagccgttg gccgatccca atcaataccg
     2701 a
//
LOCUS       DATNDD010000013        11377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_854032, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000013 DATNDD010000000
VERSION     DATNDD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854032"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VN647_00325"
     CDS             complement(<1..514)
                     /locus_tag="VN647_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG95497.1"
                     /translation="MLTTKTQAIRVLLVDDHEVIRVGLRTVLGQTQGVTVVGEAGTMA
                     EAIQQAQKLKPDVILMDVRLPDGSGVDACREILGTLPGTRVIFLTSYADDDSVLAAVL
                     AGAHGYVLKEIDSPALLEAIRSVAKGQSILDSSVTERALRWLRGLHDLPATPGTDQLS
                     SQEERVVALVA"
     gene            complement(501..6257)
                     /locus_tag="VN647_00330"
     CDS             complement(501..6257)
                     /locus_tag="VN647_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG95498.1"
                     /translation="MNPTSHRPELDLLRRQVADLARELAERDRVLHERTRHLQVAQAL
                     AHLGSWNWDIASGKMEWSDELYRIVGYEPQSREMTFDSFVSAVLPDDHDRVLVSFDDA
                     LAGVAPYDMECRIVRSDGEVRTIHCCGEVVRDGSSQPLRMSGTALDITDRKQTELALQ
                     QREAHFRALIEHSSDVITVLDPDGAIRFESPSFERLLGYAQHELDGRIAFEFVHQEDL
                     PIVIERFRLLIQRPGEPQTAEFRFRHKDGSWRSFEGIGRAIYGPDRRCSVIVNSRDIT
                     ERKRTETMLRTSQERLRQALQASGTGLWDWNTETNEVALSHEWKRQLGYEPTEISDSF
                     ETWTAHLHPDDRERAAAYARDYVSRREGEYRQEFRLRHRDGTYRWIEARASFVPEADG
                     RSVRLLGSHTDITDRKRMEESVQESEEWYRTLVELSPSGVFVFSEGRTVYVNHTGAVL
                     LGAKKIQEILDRPTFEIIHPDYHQEVRENVQRLLSGGVSVHSAERTYLKMDGTPIPVQ
                     VEAARITWNGKPAILGLFSDITERKRGQDRVREMNLAMAQAMPGISRVDLDGRYLEVN
                     QPYAAMLGYEPSELLGQTWEPTVHSEDLCIAQHAYEQLLEKGKAEFECRAVRKDGSIF
                     FTQVLMVSSKPSIGGAFGYHCFMRDITERKRTEKALAALNATLEQQVCDRTEALRQSE
                     ERFRQFFDHAPNVTALKDHNGRYVYINRQFEKVCGFSPGIAIGKTDEELFSPEQAAQF
                     QSHDQQVLLNGRGQEFEEVTHQEDGPHTSIVVRFPVTDASGRLSALGVIATDVTERKR
                     TQEALRESEVRFQQFAESVGPAFWIADLVPDGETVVYANAAFTRFRGIAGEEWSQSVS
                     RWLDSIHPDDRARVEACRTRFISGGLKAEFDCEYRMVDRDGRIRWISDRRVKMAGWGH
                     RVAGITEDITAHRQQLALLAQTEAVGKIGGWEIDFLTDHLWWSDETYRLHDTTPELYS
                     PTVDTAMNFYTPESRPVIADALQKGMKQGEAWDLELELITAKRRRIAIRVVGKVDVVN
                     GRTVRAYGTFQDITERKRAEEALRKVHDELECKVVERTAELQASEERYTRATAIGKVG
                     VWELDVLTGQYHGDANLKALFGYTPEELSTDPYAWLNLVHPDDQPIAMKNWELVQCGA
                     ADACHYELRHIRKDGSVVWGDVRAHAVRDQNGRLTNLIGATVDITERKRIEDALRDSE
                     ERFAKAFRTSPHPIGITEMATGRCIEVNDACLELFGFRREEVIGNTTLMLGVWPNPED
                     RARLVEQLKAGEPVRNKEFALRTKSGPLRYLLTSCDLVELNGTPCLITVGSDITERKR
                     AEEALRISEERFAKAFQAGPHPVVISEVDSGRVLDTNDAACQLFGYHKEEVVGRTTLQ
                     IGLWPSTQERDRFVEQLKSSGSVHNMEVTLQRKDGDVRQCLLSSELIELNGKPCSVTV
                     GDDVTERKRMERALRRTQFSVDQAVEAILWVDQTGRIFNVNETACRMLGYTRQDLTTI
                     TVHDIDPNFPVERWPEHWSHVKEQGALAFEAKFCSRTGGVLETDVTVNYLQYEGKEYG
                     CMILRDIGERKRAEAERRRSHTFLRQVIDTDPDFIFAKDREGRFTMANKAVADWYGTT
                     VENLIGKSDTDFNNIAEEVAFFRQSDLEVIHFGRDQFAPEERITDAGGRTRWLQTIKR
                     PIFDDQGRVHMVLGAATDITERKRMEEILLQRERDLSAALQERERISQDLHDGILQSL
                     YAVGLGLEACKPLIKKQQNHVTKKFMTTLEQAIGQLNQVMTEIRNFIAGLESQVMQGG
                     DFSTALRTMVETMSVSCSAKCRVKIDDAAARRLSTEQALHIINIVREGLSNALRHSRA
                     KQITVSLRDLVRSDRLAVTDDGIGFNARSAQGVGHGLVNMAARAQKVRGLFAIQSKPD
                     KGTRISLDLPKDTHYAHN"
     gene            complement(6473..6556)
                     /locus_tag="VN647_00335"
     tRNA            complement(6473..6556)
                     /locus_tag="VN647_00335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6521..6523),aa:Leu,seq:gag)
     gene            6873..7553
                     /locus_tag="VN647_00340"
     CDS             6873..7553
                     /locus_tag="VN647_00340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWG95499.1"
                     /translation="MIQTDQRGLSSSVAAFFDVDNTILPGEASELRFFRWLWRRGIVG
                     WPEARESVSWLLRHFPTLSLHPLRERKLYLAGKPSQVIQPLGEEFCREELCPRVSPAA
                     MRMLDEHRRAGHAIIFVTGSIDFLVAPVADALRADRCFASRLEQQNGLYTGFLVPPIP
                     YGEGKRRLIDRLIHELTLDLSQCYAYGDSPGDLDLLRAVGHPTVVNPIRGMASIARRK
                     NWPIVKWR"
     gene            7631..8266
                     /gene="queE"
                     /locus_tag="VN647_00345"
     CDS             7631..8266
                     /gene="queE"
                     /locus_tag="VN647_00345"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HWG95500.1"
                     /translation="MRITEIFHSVQGESTFAGLPCVFVRLTGCPLRCTWCDTEYAFFG
                     GMDWSIEDILDTVRSYGCRLVEVTGGEPLAQPDTATLLRRLCEEGFTVLLETSGAIDT
                     TIVPPSVRIILDVKCPGSGMTERMHWPNVERLRPQDEAKFVIRDRSDYEWAKTILARF
                     HLTDRCPVLFSPVFGALDPRQLAEWLLTDRLSIRLQLQLHKHIWTPDMRGV"
     gene            8361..9869
                     /locus_tag="VN647_00350"
     CDS             8361..9869
                     /locus_tag="VN647_00350"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWG95501.1"
                     /translation="MNTMRVEAKQARVDTENTNALVLLFCEGEGLPKEEGVILDRALG
                     GALRELLQSKEFDGKAGEVVVFHTHRKIPAKRLILVGLGKKHELGLDHIRQAMGHAAK
                     RIRHAKCGAFTVVLPSVVPHGASPIDVGQTMTEGAILGSYQFTTYRSDASSKDVTAMT
                     VLASRKDQLRQLSEGIRRGVATAEATAFVRDLCNHPSNVMTPTRIAQEAKTVAKAAGV
                     NLKILEQKEMEKLGMGALLGVAKGSHEPPKFIILQYRGAKKKDDRPVVLVGKTITFDT
                     GGISLKPAENMEQMKADMTGGAEVLAAMMAAARLKLPLNLISILPAAENMPGGRAMRP
                     GDVVKTLSGKTVEVQNTDAEGRLILSDGLAYATRFKPAALIDIATLTGACVVALGQFA
                     IGMFGTDAALKEAIRKAGLRAGERVWEMPLWEEYFEQLRSDVADMRNIGGRGGGMITA
                     ALFLSKFVGDAPWVHLDIASTDWSERERAYIPKGPTGIGTRLLIQFLINRSL"
     gene            9893..10990
                     /gene="nagZ"
                     /locus_tag="VN647_00355"
     CDS             9893..10990
                     /gene="nagZ"
                     /locus_tag="VN647_00355"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWG95502.1"
                     /translation="MSYDQIGQLFMIGFDGTTVSAELASFLREYRPGGIILFSRNLES
                     IAQIIELTSDLQRYSPHSPLLISVDQEGGRVSRLPKDFTIFPPCEVLGRCRSSELAYA
                     AAATTAKELKAVGINMNMSPVLDVNSNLANPVIGDRAFGTTPDIVSELGLATVGGLQD
                     NRVVACGKHFPGHGDTNSDSHKELPSVPVTRERLERVEFPPFRRAIANGVATMMTAHV
                     LYQALDENRPATLSPTIIGRFLREELHYDGVVLTDDLEMHAIIDHYGIGDAAIQAIQA
                     GCDMPLICRDRNRAVVALNAVDKAVANGDISAGRLAQSLARIRRLKERFLLPYRPVMI
                     SDAKLVVGCRSHKALLRSINQTRERFSKADL"
BASE COUNT         2419 a         3302 c         3040 g         2616 t
ORIGIN      
        1 cggcaaccag tgcaacgact cgttcttcct gagaggatag ttgatccgta ccgggggtcg
       61 ccggcagatc gtgaagccct ctcaaccacc gcagcgcccg ctcggtcaca ctggagtcca
      121 agatcgattg gccctttgca acggagcgga tcgcttccag caaggcgggt gaatcgattt
      181 ccttgaggac atagccatgg gcgccggcta gcaccgcggc gagaacggag tcgtcgtcgg
      241 cgtaggacgt caggaaaatg acgcgtgtcc ccggaagcgt ccctaggatc tcacgacagg
      301 catcgacacc ggacccgtcc ggcaaccgga catccatcaa gatcacgtca ggttttaact
      361 tctgcgcttg ttggattgcc tccgccatgg tacccgcttc ccctacaacc gtgacacctt
      421 gggtttggcc gagcaccgtc cgcaatccca cccgaatgac ctcatgatcg tcgacgagca
      481 gtactcgaat ggcttgagtt ttagttgtga gcatagtgag tatcctttgg aagatcgagt
      541 gagatcctcg tccccttgtc aggtttcgac tggatggcga acaagccgcg gactttttgg
      601 gcgcgtgccg ccatgttgac taacccatgc ccaaccccct gagccgagcg tgcattaaac
      661 ccgatgccgt catccgtgac ggcgagacga tctgaacgga cgagatcccg gagcgatacg
      721 gtgatctgtt tggcgcgact gtggcgcagc gcattgctca atccttcccg tacaatattg
      781 ataatatgga gcgcctgttc ggtggaaagc cgccgtgctg ccgcgtcatc gatcttgacc
      841 cgacatttgg cggaacagga gacggacatc gtttcgacca tggtccgcaa ggcggtcgag
      901 aagtctccac cctgcatcac ttgggattcg agtccggcaa tgaaattccg gatctccgtc
      961 atgacttggt tgagttgccc aatcgcttgc tccagtgtcg tcatgaattt cttagtcaca
     1021 tgattttgtt gcttcttgat caggggttta caggcttcta gcccgagccc gacggcatat
     1081 aaggattgga gaataccgtc atggaggtct tgactgatcc gttcccgttc ttgaagtgcg
     1141 gcgctcaagt ctcgctcgcg ttgcaggagt atctcttcca ttcgtttacg ctcggtgata
     1201 tcggtcgcgg ctccaagcac catatggact cgcccctggt catcgaaaat cggcctcttg
     1261 atcgtctgca accagcgcgt ccttccccct gcatcggtga tcctttcttc aggggcgaac
     1321 tgatccctcc cgaaatgaat cacctcaaga tcgctctgcc gaaagaacgc cacctcttcc
     1381 gcaatgttat tgaagtcagt atccgatttc ccgattaagt tttccaccgt cgtgccatac
     1441 caatcggcca cagctttgtt ggccatggta aaccgtcctt cccgatcctt ggcaaaaatg
     1501 aagtcggggt ctgtgtcaat gacctgtcgc aagaacgtat gggagcggcg acgttcggct
     1561 tccgcccgtt tgcgttctcc aatatcccgt aagatcatac agccgtactc tttcccctcg
     1621 tactgcagat aattgacggt cacatccgtt tccaagacgc ctccggttcg tgagcaaaac
     1681 ttcgcttcga acgccagcgc tccctgctcc tttacgtgac tccaatgctc cggccatcgt
     1741 tccacaggga aattaggatc gatatcatgc acggttatgg tcgtcagatc ctggcgtgta
     1801 taccccaaca tccgacatgc ggtttcattg acgttgaata ttcgaccggt ctggtcgacc
     1861 cataaaattg cctccactgc ctgatccacc gaaaactgag tccgccgtag cgctcgttcc
     1921 atgcgtttgc gctccgtgac atcgtctccg accgtcacag agcatggctt cccattcaac
     1981 tcaatgagct ccgacgacaa caggcattga cggacatcgc catccttcct ctggagcgtc
     2041 acctccatat tgtgaactga tccggaactt ttcagctgtt cgacgaaacg atcccgttcc
     2101 tgcgtcgagg gccagagccc aatctgtaat gtcgtgcgtc ccaccacctc ttccttgtga
     2161 tatccgaaca actgacaagc ggcatcattg gtgtccaaca ctcgaccgga gtccacttca
     2221 ctaatgacga ccggatgggg accagcctgg aacgccttgg caaaacgctc ctcactgatt
     2281 cgtagcgctt cctctgctct ctttctctcg gtgatgtcgc tcccgacagt aattagacac
     2341 ggtgtgccat tcagctccac aaggtcacac gaagtcagaa ggtaacgcaa tgggccggac
     2401 ttggtcctta aagcaaactc cttattgcgc accggttctc cggctttcag ctgctcgacg
     2461 agccgcgctc gatcttcggg attcggccag acacccaaca tcaatgtggt gttcccgatc
     2521 acttcctctc gccgaaaacc gaacagctcc aaacaggcat cgttcacctc gatacaacgg
     2581 cccgtggcca tttccgtgat accgattgga tgcgggctcg tgcggaaagc tttggcaaat
     2641 cgctcttcac tatctctcaa cgcatcttct atccgcttac gctcagtgat atcgacggtg
     2701 gcgccgatca agttggtcaa tcgtccgttt tgatctcgga cagcatgggc tcgcacatca
     2761 ccccaaacaa ccgagccgtc tttccttatg tgacggagct catagtgaca tgcatcggct
     2821 gctccacatt gcacgagttc ccaattcttc atcgcgatcg gctgatcatc cggatggacc
     2881 agattcagcc aagcataggg atccgtggag agttcttccg gcgtataccc aaagagagct
     2941 ttcagattcg cgtccccgtg gtattgacct gttaggacat ccagttccca gactccaacc
     3001 ttaccgatag cagtcgcacg cgtataacgt tcttcgctgg cctgcaactc agcggtgcgc
     3061 tcaacgactt tgcactccaa ttcatcgtgg acctttcgaa gagcctcttc ggcccgtttg
     3121 cgctcggtaa tgtcttggaa cgtgccgtac gcccgaaccg tccgtccgtt caccacatcg
     3181 accttcccaa cgacccgtat ggcaatccgg cgcctcttcg ccgtgatcag ttccaactcg
     3241 agatcccacg cttctccctg tttcatccct ttctgaagtg catcggcaat aacgggtcga
     3301 ctctcgggtg tatagaaatt catcgccgtg tccaccgtcg ggctgtacag ctccggtgtc
     3361 gtatcatgca accggtatgt ttcgtcactc caccagaggt ggtcggtcag aaaatcgatt
     3421 tcccatcctc cgatttttcc aacagcttct gtttgggcca gcagggcaag ctgctgtcga
     3481 tgggcggtaa tatcttccgt aatgccggcg acacgatggc cccacccggc cattttgact
     3541 ctacggtcgg aaatccaccg gatacgtccg tcccgatcca ccatccggta ctcgcagtca
     3601 aactcggctt tcaaccctcc cgagataaat cgtgtgcggc aagcctccac tcgcgcccga
     3661 tcgtcgggat ggatcgaatc gagccaccgt gacacgcttt gcgaccattc ttcccccgcg
     3721 attccccgga agcgggtaaa cgcggcattg gcgtacacga ccgtctcgcc gtccggtaca
     3781 agatccgcta tccagaatgc cggaccgacc gactctgcga actgttgaaa tcggacttcg
     3841 ctctcgcgca acgcctcctg tgttcgcttc cgttccgtaa catctgtggc aatgacgcct
     3901 agggcagaga gacggcctga agcatcggtg accgggaatc tcaccacgat actggtatgg
     3961 ggtccatcct cctggtgcgt cacttcctcg aattcctgtc cccggccatt gagtaacacc
     4021 tgttgatcgt gtgattgaaa ttgggccgcc tgttcgggag agaataattc ctcatcggtc
     4081 ttccctatgg ctatgcctgg agaaaatccg cataccttct cgaactgtcg attgatatat
     4141 acataacgcc cgttgtgatc tttcagagca gtcacattcg gcgcatggtc gaaaaactga
     4201 cgaaaccgtt cttcactctg gcgcagcgct tcggtccgat cgcatacttg ctgttccagc
     4261 gtggcgttaa gagcggccaa cgctttctcc gtccgttttc gttcggtgat gtcgcgcatg
     4321 aaacaatggt agccaaacgc accgccgatg gacggtttgc tacttaccat caggacctgc
     4381 gtaaagaaga tggaaccatc ttttcgtaca gctcgacact cgaactccgc tttccccttt
     4441 tccagcaact gttcataggc atgttgggcg atgcacaggt cttcagaatg gacggtcggc
     4501 tcccatgttt gtccgagcaa ctcggacggt tcatacccca gcatcgccgc gtagggctga
     4561 ttcacttcca ggtagcggcc atctaagtca actcgggaaa tgcccggcat ggcctgagcc
     4621 atggccaggt tcatttcccg cacacggtct tgaccccgct tacgttcggt gatatcggag
     4681 aacaggccga ggatggcggg cttaccgttc cacgtgatcc gagctgcctc gacctgaacc
     4741 ggaatcggcg ttccatccat cttcaaatac gttcgttccg cactatggac ggacacgccg
     4801 ccgctcagca gccgttgcac attctcacgg acttcttgat gatagtccgg atggataatc
     4861 tcaaatgtcg gccggtcgag aatttcctga atctttttcg ctcccaggag cacggcgccc
     4921 gtgtggttga catagactgt tcgtccttca ctgaagacga atacacccga tggtgaaagc
     4981 tcgaccaaag ttctgtacca ttcctcactc tcctgcaccg actcctccat ccgtttacga
     5041 tcagtaatat cggtatgtga gccgaggaga cgaacactcc ggccatccgc ttcgggcaca
     5101 aaggaggccc gtgcctcgat ccatcggtaa gtcccatccc tatgccgcag acgaaactcc
     5161 tgccgatact ccccctcgcg ccgagacacg tagtctcggg catacgctgc cgctctctct
     5221 cgatcatcgg ggtgcagatg cgctgtccag gtttcaaatg aatctgagat ctccgtcggc
     5281 tcatagccga gctggcgttt ccattcgtgg gacaaggcaa cctcgtttgt ttctgtattc
     5341 cagtcccata aacccgtacc ggacgcctga agggcttgtc ggagcctctc ttgcgatgtc
     5401 cgcagcatcg tctctgtgcg ctttcgctca gtaatgtcgc gtgaattcac gatgacgctg
     5461 cagcggcggt ctggaccata gatcgcccgg cctattcctt cgaaactgcg ccacgagccg
     5521 tccttgtgcc gaaaacggaa ttcggccgtt tgaggttctc ctggccgctg gatgagcaac
     5581 cggaacctct cgatcacaat aggaaggtcc tcctgatgaa cgaactcgaa agcgatacgt
     5641 ccatcgagct cgtgttgtgc atatccaagc aaccgctcga aggatgggct ttcgaaccgg
     5701 attgcgccgt ccggatcgag aactgttatg acgtcggagg aatgttcgat gagcgcgcgg
     5761 aagtgcgctt cgcgctgctg cagggcaagc tccgtttgct tgcgatcggt aatgtccaag
     5821 gccgtcccgg acatacgaag gggctgactg ctgccgtcac gcacgacctc gccgcagcaa
     5881 tgaatggtcc gtacttcgcc gtcggagcga acgattcggc attccatgtc gtatggtgcg
     5941 acccctgcta acgcgtcatc gaacgacacc aggacccggt catgatcgtc cgggagtacg
     6001 gccgacacaa aactgtcgaa ggtcatctct cgtgattgag gctcatagcc gacaatccgg
     6061 tagagttcat ccgaccattc catcttgccg cttgcaatgt cccaattcca actccctagg
     6121 tgagcaagag cttgtgcaac ctggaggtga cgggtccgtt cgtgcaacac tcggtcccgt
     6181 tcggcaagtt cacgtgcgag atcggcgacc tgcctccgca gcagatccaa ttcaggacga
     6241 tgactcgtag gattcatgat gaggctcctt gccgcgcgac gccgacagta cctcaaaagt
     6301 cagctcaccg aagtgaccct gtcacgtcgc gagtgatcaa tgaaggggcc agccagccag
     6361 ttcctaagaa agcccctgag agtggattag tgtcctatgg gaataggtga aagtctagat
     6421 ccaaatggcc cggagtcttt agctggcaat ctgcgggagc tgtttacttt gatggtgccg
     6481 aaggcgggac ttgaacccgc acggctttcg ccacacgccc ctcaaacgtg cgtgtctgcc
     6541 attccaccac ttcggcactc agcaggatgc tgaaacgaaa cctgccgctg cgttctcgca
     6601 tcgctcaaac cttcaccgta cttgaaagta cgcctcaggc aagacacagc cggcgcacca
     6661 tctcgccggc gcgcacaaac ttggtgtcgc ttattcgcga cactgtgcgc ctcgttgcgg
     6721 ccttgtcgca ggtttgtttg accatctgca tcaagacctc ctgtcagcta agaggggaaa
     6781 ggagtgcgca ttatagaagt agggtaaaat tcttgtcaat caaccggtcg tccggtagaa
     6841 tcagcacctc gttgtcctag ggcactcttc gtatgataca aacagaccaa cgaggtctct
     6901 cttcttccgt tgcggcgttt tttgatgtcg acaataccat tttgccgggc gaagcgagcg
     6961 aattgagatt ttttcgctgg ctgtggcggc gcggtatcgt cggttggcct gaagctcgag
     7021 agagtgtgtc ttggcttctg cgacatttcc cgacattgtc gctgcatccg ctccgcgagc
     7081 ggaagctgta tctggccggc aaaccttcgc aagtgatcca gccgttgggc gaggagtttt
     7141 gtcgcgagga attatgtcct cgtgtctctc ccgccgccat gcggatgctc gacgagcatc
     7201 gtcgtgccgg ccatgccatt attttcgtga cggggtccat cgatttcctg gtcgcccccg
     7261 tcgccgacgc gctccgagct gatcgatgtt ttgcgagtcg gttggagcaa cagaacggcc
     7321 tctataccgg tttcctcgtc ccccccattc cttatggaga agggaaacgc cgactcatcg
     7381 atcggttgat ccatgaactg acgctggatc tgtcccaatg ctatgcgtac ggagacagtc
     7441 ctggtgattt agacctgctc cgggccgtag gtcatccgac ggtggtgaat ccgattcgcg
     7501 gcatggcctc catcgcccga cgcaaaaact ggccgatcgt caaatggcga tgagctgctg
     7561 cctgtgatcg gaccaaccgt ctctgtggcc aatcattcaa tcgcgcactc ggtaacccca
     7621 catactgctc atgcgcatta ccgaaatttt tcatagcgtt caaggtgagt cgacctttgc
     7681 cgggctgccc tgcgtatttg tacgattgac cggttgccct ctacggtgca cctggtgtga
     7741 tacggaatat gctttcttcg gcggaatgga ctggtccatc gaggacattc tcgacacggt
     7801 gcgatcctat ggttgtcggt tggttgaagt gaccggaggt gagcccttag cacaaccgga
     7861 cacggctacg ctgctgcgtc gattgtgcga agaaggcttc accgtcctcc ttgaaacaag
     7921 cggagcgatc gatactacca tagtcccccc gtcggtccgt attatcctgg acgtgaagtg
     7981 cccgggaagc ggcatgacgg aacggatgca ctggcccaat gtcgaacgtc tacgaccgca
     8041 agacgaagcc aaatttgtta tccgggaccg gagcgattac gaatgggcta aaaccattct
     8101 ggctcgcttc cacttgaccg atcgctgtcc ggtcctcttt agtccggtat tcggtgcgct
     8161 ggatccacga caattggccg aatggctcct gacagatcga ctatctattc gtcttcaatt
     8221 gcaactccac aagcacatct ggacgcctga tatgcgtggg gtgtgaaaaa cgttctctgt
     8281 cgatcgggat atacacctca tggtggcaca catcaatcgg ttgatgatgc ttgctgactg
     8341 atacatcaaa ggagaatcgg atgaacacta tgcgagttga agcgaaacaa gcccgcgtcg
     8401 acaccgagaa cacaaatgcg ctggtcctcc tgttttgtga aggggagggg ctcccgaagg
     8461 aagagggggt cattctcgat agagcgctcg gaggggcact gcgagaactt ctgcagtcaa
     8521 aggaattcga tggaaaagcc ggtgaggtag tcgtatttca tacacaccga aagataccgg
     8581 cgaagcggtt gattcttgtc ggccttggga agaaacatga gctgggtttg gatcacatcc
     8641 gacaggcgat ggggcatgcc gccaaacgga ttcgccatgc caaatgcgga gcctttacgg
     8701 ttgtgttgcc gagtgtcgtg ccacatgggg cctcgccgat tgacgttggc cagacaatga
     8761 ccgaaggggc tattttaggg agctatcagt tcacgacata tcgcagcgat gcctcgagca
     8821 aggacgtgac ggcgatgacc gttctggctt cgcggaaaga tcagctgcgc cagttgtccg
     8881 aagggattcg tcgcggtgtc gcaactgctg aagcgacggc cttcgtgcgg gacctctgca
     8941 accatccgtc caacgtcatg acaccgacca ggattgctca agaggcaaag accgtcgcga
     9001 aggcagccgg tgtgaatctg aaaatccttg aacaaaagga gatggaaaaa ctgggtatgg
     9061 gtgccttgct gggggtcgcg aaaggcagcc acgaaccgcc gaagttcatt attctccagt
     9121 accggggagc caagaaaaaa gacgaccgcc cggtcgtgct cgtcggtaag acgatcacct
     9181 tcgataccgg agggatctcg ctcaaaccgg cggagaatat ggaacagatg aaagccgaca
     9241 tgaccggtgg ggctgaggta ttggccgcaa tgatggcggc ggctcgtctg aagttacctc
     9301 ttaatctcat cagcatccta ccggcggcgg aaaatatgcc cgggggacgg gcaatgcgac
     9361 ctggtgacgt agtcaagacc ctttctggga aaaccgtgga ggtgcagaac acagacgccg
     9421 agggccgcct gatcctatcc gacggcctcg cctacgcaac tcggttcaag ccggcggcac
     9481 tcatcgacat cgccacgctg acgggagctt gtgtcgtcgc gctgggccag ttcgcaatcg
     9541 gaatgttcgg taccgatgcg gcgttgaaag aagcaattcg caaggccggc cttcgagcag
     9601 gcgagcgggt atgggaaatg ccgttgtggg aggaatattt cgagcaactg cgcagcgatg
     9661 tggccgacat gcggaacatc ggtggacggg gaggtggcat gatcacggct gctctcttct
     9721 tgagcaagtt cgtcggcgac gccccttggg tacatcttga catcgccagc accgattgga
     9781 gcgagcgaga gcgggcttac atccccaaag gcccgaccgg tatcgggaca aggctgttga
     9841 tccagttcct catcaatcgc tccctgtagg gcgagcgcaa ggcggttcga agatgtctta
     9901 cgaccagatc ggccaactct ttatgatagg gttcgatggc accaccgttt cggctgaact
     9961 cgcttccttc ctccgggaat ataggcccgg tggcatcatc ctgttttcga gaaatctcga
    10021 atcgatcgca cagatcattg aattgacaag cgatcttcaa cgctatagtc cgcactctcc
    10081 ccttcttatc tctgtcgatc aagaaggcgg acgtgtctca cggttgccga aagactttac
    10141 gattttccca ccgtgcgaag tcctggggcg atgccgctcc tccgagctgg cctacgctgc
    10201 agcagcgacg accgcaaaag agctcaaggc cgtcggcatc aatatgaata tgtctccggt
    10261 gctggatgtg aacagcaacc tcgccaaccc cgtaattggg gatcgagcat tcggcacgac
    10321 tcctgatatc gtgtctgaat tggggttggc cacagtggga ggcctccagg acaatcgtgt
    10381 cgtggcttgt gggaaacact ttcctggcca tggcgatacc aactcggact cccacaagga
    10441 gttgcccagt gtgccggtca cgcgtgaacg acttgagcgc gttgaatttc ctccattccg
    10501 gcgcgcgatt gccaacggcg tggcaacgat gatgacggcc catgttttgt atcaggcttt
    10561 ggatgagaac cggccggcaa ccctgtctcc gacgatcatt ggaagatttc ttcgtgaaga
    10621 gttgcactac gacggcgtcg tgttgacgga tgatctcgag atgcatgcca tcattgatca
    10681 ctatggcatc ggcgacgccg cgatacaggc catccaagcc ggctgcgaca tgccgctgat
    10741 ctgtagagac cgcaacagag ccgtagtcgc gctgaatgcc gttgataagg cggtagcaaa
    10801 cggggacatc tctgccggac ggttggccca gtctcttgca cgtattcgtc gcttgaaaga
    10861 acggttcctc cttccctatc gacccgtgat gatttctgac gccaaacttg tggtcggttg
    10921 ccgaagccac aaggctcttt tacgctccat caaccagaca cgagaacgat tttcgaaagc
    10981 cgacctatga cctttgggag aattggctaa gctctaccaa gatggaagac ctcctctcaa
    11041 tgcagtgagg aaacgaaact tctgacctcc ccgctgaata tcttgccctc ttctcatctc
    11101 ttgtggcatc atgaacgttc cttggagggc cttatggcct cattgcttga gtacgttgga
    11161 tccgtccata ttttcctggg accctaccgg ggtaatccaa tcgctttgta tttgcggagg
    11221 acggaatcag gctgccagat cggacccaag gtctatcctt ggaacgatgt catgggcgtc
    11281 ggcgaaacac cgaataaggc cgcagctgat ttcgaggaaa aatggaaaac aaagggctta
    11341 acggccgata tgtattcggg tccggcttgg gaaggag
//
LOCUS       DATNDD010000014         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2567829, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000014 DATNDD010000000
VERSION     DATNDD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567829"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VN647_00360"
     CDS             complement(<1..686)
                     /locus_tag="VN647_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HWG95503.1"
                     /translation="MLGICDPLLAVSSMLLGGAAAYVILSIAERMRAPDQGPVKMRWL
                     TIGAVAAGLEIWAMHYIGNLAFCPPVPAPQDSALAVLSLLSAAVTGAVAVYLISSHVD
                     SRMRLVSGGMLMGACMNLTHFSSMLAVHQPIDLQNGVLLFVLSVVGIVVLSIIVLAVG
                     AWNISYGNWRVTEKASAMGLALSGSHLIGMISTYGISNVTTRAPPPGIEVDLLAVVAV
                     SLSTLLAIIDR"
     gene            complement(803..1864)
                     /gene="lepB"
                     /locus_tag="VN647_00365"
     CDS             complement(803..1864)
                     /gene="lepB"
                     /locus_tag="VN647_00365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWG95504.1"
                     /translation="MRYPPRIHEREEYRLNGKILSRLSRILDRSSNRVAVCLLVTCNG
                     FWAGCGTTPLVQSDRDSESQAVFHQLAGLEMASDRLYESIADQADVAGFSHQLEVGSD
                     LASPFTSSEKERIAQSFVRSLKVMLFDLAPTNFWEDHLAQYYASTLPAPEARRLVEGY
                     GSGILIPTSKMQELRRNFVTDRLPDLLSAVRAQSHLFHISNEAMVPALLSGDHVITHR
                     TAYHAAEPQRGDVVVYRYPDENGTLFLHRVIGIPGDRIAIHNQEVSVNEDTVTEPYVQ
                     HTDRSNMVGNVRDNFGPVTVPPDAYFLLGDNREESLDSRFLGPISREYILGQARFIYW
                     SVDPVTRNPRWDRLNQPVH"
     gene            complement(1990..>2175)
                     /locus_tag="VN647_00370"
     CDS             complement(1990..>2175)
                     /locus_tag="VN647_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95505.1"
                     /translation="MRSWAFAQQGQRLCEAVTDIMAGKRQYRDLRFKPQTLFRLLSPK
                     WFRNSVHRLARPRDDQT"
BASE COUNT          493 a          642 c          630 g          410 t
ORIGIN      
        1 cgatcgatga tggccaggag cgtcgagagc gatactgcga caactgcaag gagatctacc
       61 tcgattcccg gcggcggagc cctggtcgtc acgttggaaa taccataagt cgaaatcatc
      121 ccgatgaggt gggacccgga aagagcaagc cccatggcgc tcgccttttc cgtcacgcgc
      181 cagtttccat acgagatatt ccaggcgccg accgcgagga cgatgatgct cagtaccacg
      241 atccccacaa ccgacagcac gaagagcaac acgccgtttt ggaggtcgat cggctggtgg
      301 accgccagca tgctggagaa atgggtcagg ttcatgcagg cacccatgag cattcccccg
      361 gagacgagtc gcattcggct gtcgacgtgg ctgctgatca ggtatacggc cacggctccc
      421 gtcaccgcgg cggacagaag ggaaagaacg gcgagcgccg agtcctgggg tgccgggacc
      481 ggcggacaga acgccagatt cccgatgtag tgcatcgccc atatttcaag gccggctgcg
      541 acggcaccga tcgtgagcca tcgcatcttg acgggtcctt ggtcgggcgc gcgcatccgt
      601 tcggcgatgc tgagaatgac ataggcggcc gcgccgccca gcagcatcga ggaaacggca
      661 agtaagggat cgcagattcc aagcacggtg aacccactca ggtgacgtca atgagcatcg
      721 tagacatgat ggcaagtgcc gggattttgg accatggcgg caaggcggcg tcgaacctga
      781 tgcggatatc atctaccatg ggtcaatgca ccggttgatt gagtcgatcc cagcgaggat
      841 tccttgtgac gggatcaacc gaccagtaga tgaaccgcgc ctgccccaga atgtattccc
      901 tgctgatcgg tcccaagaaa cggctatcca gactctcttc ccggttgtca cccaagagga
      961 aataggcgtc tggaggcacg gtcacaggcc cgaagttatc acggacgttt ccgaccatat
     1021 tggatcggtc agtatgttgc acatagggtt cggtcacggt gtcttcattc acggagacct
     1081 cttgattatg tatggcgatc cgatcgccgg gaatcccgat cactcggtga agaaagagtg
     1141 ttccattctc gtccggataa cgatagacga cgacatcccc gcgttgaggt tcggctgcgt
     1201 gatacgccgt cctgtgcgta atcacatggt ctcccgacaa gagcgccggg accatcgcct
     1261 cgtttgagat atggaacaga tgcgactgcg ctcgcaccgc cgacaacagg tccggcagac
     1321 ggtccgtcac aaaattccgg cggagttcct gcatcttcga cgtcgggatg aggatcccgc
     1381 tgccgtaccc ctcgaccaac cgccgggcct ccggggccgg caacgtcgag gcatagtatt
     1441 gcgcgagatg gtcctcccag aaattcgtcg gggccagatc gaacagcatg actttgagcg
     1501 agcgcacaaa cgactgcgcg atgcgctcct tctccgacga agtgaacgga ctggcgagat
     1561 cagacccgac ctccaactga tggctgaacc ccgccacgtc ggcctggtcc gcaatcgact
     1621 cgtacaaacg atccgatgcc atttcgaggc cggccaactg atgaaaaaca gcttgcgact
     1681 cgctgtcacg gtcggactga actagaggag tagtgccgca cccggcccag aatccattac
     1741 atgtcacaag cagacaaacg gcgacgcgat tcgaagatct atcaagaatg cgagatagcc
     1801 gggagagaat cttgccgttc agccgatact cctctcgttc atgtatccgg ggaggatacc
     1861 gcaccgggcc tcgataggaa aggccactcg ggcagaccgt acgccacgcg tattcaacaa
     1921 cgcgttcgaa aaattgaata gtgccggcca agatgctgca gcaagctccc catccctgct
     1981 cggcctgtat caggtctgat catcgcgcgg ccgagcaagc ctgtggaccg agtttctgaa
     2041 ccattttggc gatagcagcc ggaacagggt ttgtggcttg aacctcaggt ctcgatactg
     2101 ccgttttcct gccatgatgt ccgtcaccgc ttcacagaga cgctgtccct gttgtgcaaa
     2161 cgcccatgac ctcat
//
LOCUS       DATNDD010000015         5209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2296153, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000015 DATNDD010000000
VERSION     DATNDD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296153"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..340)
                     /locus_tag="VN647_00375"
     CDS             complement(95..340)
                     /locus_tag="VN647_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95506.1"
                     /translation="MDFVYTHGMSDRSDSFVSFSTESRRIFYEDHRSLALFMILIVFA
                     APFAGLYVAGLLGVVVGVLVSAAAYVLTPCLWKWLGG"
     gene            complement(396..1157)
                     /locus_tag="VN647_00380"
     CDS             complement(396..1157)
                     /locus_tag="VN647_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HWG95507.1"
                     /translation="MTSTVPPFTDTQATLLSRFLSSPLRPKDSLTYPQLAGFLFCLAN
                     GPELIPPSEWMPLIFNDQDAGYETQEEAERVLQAIMALYNDCIRERTVGGVSLPPGCE
                     VRPWAMDNLDVEAPLSQWAQGFGIGYDYLVEVWNEYTPDELDKELGAVLMTLTFFTSP
                     QLAQAYHQETKGKEGSLDQLAQSVMEIFPEAMREYAHLGRSIYQARLEVGTLDLEHSA
                     HTKIERNDPCPCGSGKKYKKCCGLTKGTSSGQVFH"
     gene            1434..2156
                     /locus_tag="VN647_00385"
     CDS             1434..2156
                     /locus_tag="VN647_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95508.1"
                     /translation="MISILRMIVMRTHGPRGAVFSRTRSTHRLIHRACLATALLVTIA
                     VVAQAVQAAGGGQTKFKRISTQYIAALGDPGATSGNGAQLWGLWPLDPGPRGVKLNGY
                     QSLKDAGGIAPARWKFDETDWWLEEHGLIMEQPTVPLLPGKYLVTGARKVTAVLTIHP
                     ADSHGDTRWELDQGATLYDVTHLACRSARYTPATVGGSCSPANAKQTAFPVAPGGAMP
                     PVEGCTKQDYAVLIVIGMGVED"
     gene            2206..3480
                     /locus_tag="VN647_00390"
     CDS             2206..3480
                     /locus_tag="VN647_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95509.1"
                     /translation="MVAEQRPGGVGVNKALTIGKIYKEIAGDKVVFSSQIDFGNQTQT
                     LWYRLDAEYSDYFCDDRGDGLVASLLFFSLMNGADIVSTLPISEKLYYQLTYNLIPQL
                     CRCNKSVISPIKIEASLVSTGYPKASGVGCGISCGVDSFTTLYEYTRLVPLDSFKLTH
                     LTYLKVGAHDGQIGKYDKEVQEGLFQSQLSHAKAFCTRYGYKLIVVESNLTEIIDRMF
                     GFYNYEQFHSYVNAGTVLQLQKLFHRYYYSPGYCLDQFLVDVKKDAAHYEHVLLPMLS
                     TETTSFYSSNQAMNRIEKERLISDFEQSYEWLLVCWLHKPNCGKCRKCIRTMVGFDFV
                     GKLELYRACFDLDEYFKKKDQYLRMIVQTRERDFSMKELYDYARAHNIALPSSENKNR
                     RNIINRVFDSLGQRGVRQTLSIALQRLMRRES"
     gene            complement(3752..4087)
                     /locus_tag="VN647_00395"
     CDS             complement(3752..4087)
                     /locus_tag="VN647_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95510.1"
                     /translation="MQAVTLLFLAGLLFATGCNRGLGGPLDAASLDPNQDHLKIASFY
                     SRQAMLSRQKAAEQASRALTYEQLFGRDSDWVAGARMLTRFYEDEAREQDRQAGLHLE
                     LAGRRPSNR"
     gene            4272..>5209
                     /locus_tag="VN647_00400"
     CDS             4272..>5209
                     /locus_tag="VN647_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95511.1"
                     /translation="MKRIVTALLSVLWLGGCGVLVPYMYDAQILQDRLVVSMPKEQVL
                     KQLGKPHRVVREEGRQIILEYRFYPQGDWTAYLIHCPLFPNCYFPGEAGHPYYVVLQD
                     NQLCLWGTPDVVRPLLGLVCGPTVPLGRKEHVRGGLHISVIPVFMPPPIVPLPQRLAI
                     IPVDGSSDFEISSWLDLTLNFLRTRHPDLVLVEREDLKAVLNEMGIQYGGHVDDETSI
                     HIGRLVGADSLLTYRMIVPEDMPMSAAFELRLFKVESGTTLFRQTVSASQVSPGAVVT
                     RAQGSAELDSLARRPVLQEAAAYGLAAFTAAFGDNP"
BASE COUNT         1170 a         1411 c         1411 g         1217 t
ORIGIN      
        1 cgaagtctgg accttcatgc gtgtgcggga tggacgttgg ctcctatcgg ccatccaaca
       61 ataacgtctg agcaaaggcg gctgattcac aaaatcagcc gccgagccac ttccagagac
      121 agggcgtgag tacataggcc gcagccgaga ccagcacccc caccaccacg cccaaaagcc
      181 ctgctacata gagtcccgca aatggtgctg caaacacaat caggatcatg aacagggcca
      241 gcgaccgatg atcttcgtag aatatgcggc gagactcggt tgaaaaggaa acgaaggagt
      301 cggatcgatc agacatgccg tgagtataga cgaaatccaa cgtcctgtct gtccggaagt
      361 ctcagagaat gagcaccctt catcagcaca atgtgtcagt ggaagacctg gccggaactc
      421 gttcctttcg ttagtccaca gcacttcttg tacttcttcc cgctgccgca ggggcaggga
      481 tcatttcgtt cgatctttgt gtgagctgag tgttctagat cgagggtgcc tacttcaagt
      541 cgcgcctgat agatcgatcg tcccaggtga gcatattcgc gcatcgcctc aggaaagatc
      601 tccatcaccg attgggccaa ctggtccaga ctcccctcct tccccttcgt ttcctgatgg
      661 tacgcctggg ccagttgcgg cgacgtaaag aacgtgagcg tcatcagcac cgctccaagt
      721 tctttatcga gttcatccgg cgtgtactca ttccacacct ccacaagata gtcatagccg
      781 attccaaacc cttgcgccca ctgactcagc ggtgcctcga catccagatt gtccatcgcc
      841 cacggtctca cctcgcaccc cggcgggaga gaaacacctc caacggttcg ttcacgaata
      901 caatcgttgt agagcgccat tatcgcctgg agcacccgct ctgcttcctc ttgcgtctca
      961 tagccggcat cctgatcgtt gaagatcagc ggcatccatt cggaaggtgg aatcaactcc
     1021 ggcccattcg ccaggcagaa caaaaatccc gccagttggg gataggtcag ggagtccttt
     1081 ggacgcagcg gtgaagataa gaagcgcgat aagagtgtcg cttgggtgtc tgtgaatggt
     1141 ggaaccgtgc tcgtcatgac ggagtcagtc tgtcagttgt tggtagtgca tttcaatcct
     1201 tccttggtct ccgctgactg tagctcagct tcacctactc atcggctgct tgagataccg
     1261 cacatgtgat tcacaaagac tccagtcccc atttcttctc tccaagcagc ctcgctgaag
     1321 gaccggtcct acatctcgag gttgatgtca ttctggatat ccatacggcc ggcctggtaa
     1381 aagatagcgg tgatctggtt gttgctgacc cgccctgaaa ggaacgtcgc acgatgatat
     1441 caatcctcag aatgatcgtg atgcgtaccc atggaccgcg aggtgccgta ttctcgcgca
     1501 cgaggagcac acatcgactc attcaccgtg cctgccttgc gacggcattg ctggtgacga
     1561 tcgccgtggt ggctcaggcg gtgcaagccg caggcggagg ccagacaaag ttcaagcgca
     1621 tctcgacgca atacatcgcg gctttgggcg atccaggtgc gacctccggg aacggcgcgc
     1681 aattgtgggg tctgtggccg ctggacccgg gtcctcgtgg cgtgaagctg aacggctatc
     1741 aatcgttaaa ggatgccggt ggcatcgccc cggcgcgctg gaagttcgac gagacggact
     1801 ggtggctgga ggaacacggc ttgatcatgg aacaaccgac cgttccactc cttcccggca
     1861 aatatctggt cacgggcgcc cgcaaggtga cggcagtgct gactattcat cccgccgaca
     1921 gccatggcga cacacgttgg gagctcgatc aaggcgcgac actctatgac gtgacgcacc
     1981 tggcctgccg ttccgcacgc tatacaccgg cgacggtggg tggttcgtgc tcaccggcta
     2041 atgcaaagca gacggcgttt ccagttgcac caggaggcgc gatgcctccg gtcgagggct
     2101 gcacgaagca ggactatgcg gtgctcatcg tgatcggcat gggcgtggaa gactgaacaa
     2161 aagttaacgt tacccaacaa gaacgtcggg cgtgaacgaa cttgaatggt ggccgaacag
     2221 cgtccagggg gtgtgggggt gaataaggct ttaaccatag gaaaaatcta taaagaaatt
     2281 gctggggaca aggtggtttt ttcttcgcaa atagatttcg gcaatcaaac acagactctg
     2341 tggtatcgtt tggatgcaga atacagcgat tatttttgcg atgatcgggg cgacggatta
     2401 gttgcaagcc ttttgttctt ctcgttgatg aacggcgctg acatagtcag tactctgccc
     2461 atttccgaaa agctgtacta tcaactcacg tataatctaa ttcctcagtt atgccgctgc
     2521 aacaagtcag tcatttctcc gataaagatc gaagcgtcgc ttgtttcaac agggtatccg
     2581 aaggcgagcg gggtcggctg tggaatatct tgcggtgtcg actccttcac cacactgtat
     2641 gaatacacta gactcgttcc tcttgattca ttcaagttaa cccatctcac ctacctgaag
     2701 gttggcgctc atgacggcca gattgggaaa tatgacaaag aagtgcagga aggccttttc
     2761 caatctcaat tatcacatgc caaggcattc tgcactcggt acggatacaa gcttatcgta
     2821 gtggagtcca accttacaga aataatagat cgcatgttcg gtttctataa ttatgaacag
     2881 tttcattctt atgtgaacgc cggaaccgtt ctgcaactgc agaaactctt tcatcgatat
     2941 tactactctc caggctattg tcttgatcag ttccttgtgg atgtaaaaaa ggatgcagct
     3001 cattacgagc atgttctgct cccaatgtta tccacagaaa cgacaagttt ttacagctcc
     3061 aaccaagcga tgaatcgaat tgaaaaagag cgcctgatct ccgactttga gcagagttat
     3121 gaatggctat tggtatgctg gctacacaaa ccaaattgcg gaaagtgcag aaaatgcatt
     3181 cggactatgg tggggttcga ttttgttggc aaacttgaac tttaccgagc ctgttttgat
     3241 ttagatgaat atttcaaaaa gaaagaccaa tatctgagaa tgattgttca gacgagagaa
     3301 cgcgactttt ctatgaagga gctctatgat tatgcaaggg ctcataatat tgcgcttccc
     3361 tcatccgaga ataagaaccg acgaaatatt attaatcggg tgtttgattc tttagggcaa
     3421 agaggtgtgc gccaaactct atctatagcc ttacagcgct tgatgcggcg tgagtcgtga
     3481 tttcgacgcg ctttcgcctg gcacgccaag accgcctgcc ttgcatgcgg gcgtgagggc
     3541 aaagcgtggc ggtcgcggta ccgggagtct ttcttgccat taagggtgcc agttcaccag
     3601 cctttctatc cacattcttc cagaagaatc tttcagatga tccgcccgag gcactcggga
     3661 cagagttgct ccgggcacgc ggttggcggg cacctgacac atgggaaaca ttgatgcaca
     3721 ggcccccaga acccatactg gccggttgaa cctaacgatt cgagggtcgt ctgccggcta
     3781 gttccagatg caggccggct tgtcggtcct gttcgcgcgc ctcgtcttca taaaaccgcg
     3841 tgagcatccg cgcaccggcc acccaatccg aatcacgtcc gaagagctgt tcataggtca
     3901 aggcgcgact ggcttgttcc gccgcctttt ggcgggacag catagcctgt cgggagtaaa
     3961 aggaggcgat tttcaaatgg tcttgatttg ggtcgagtga ggcagcgtca agcggccctc
     4021 ccaggccccg attgcatccg gttgcgaaca ggagaccggc taagaacagg agcgttacag
     4081 cctgcatact gtccaccctt tcatcggccc gtattatgct atcgcacgcc tgcgcgatca
     4141 actaaagggt gccgctcata tcacgcaccc tttcggcatt gatttccctg gcccatgggc
     4201 tctctagctc agctccacag tccggcgtac aatcaatcga gtgggatcgt cgttggaccg
     4261 gaggaaccgc catgaaacga attgtgaccg ctcttctgtc ggtgctgtgg ctggggggat
     4321 gcggcgtcct cgttccatat atgtacgatg cccagatatt acaagatcgg ttggtcgtgt
     4381 cgatgccgaa ggaacaagtc ctcaagcagt tgggcaaacc gcatcgtgtc gttcgggagg
     4441 aagggcggca gatcattttg gagtatcggt tctatccaca gggcgattgg accgcctatc
     4501 tgatccattg tccgcttttt ccgaactgct actttcccgg cgaagcgggg cacccgtatt
     4561 atgtcgtgtt gcaggacaat caattgtgcc tgtggggaac gcctgacgtc gtccggcccc
     4621 tgcttgggct cgtctgcggg cctacggttc cgctcggtcg aaaagagcat gtccgtggag
     4681 gccttcacat ttcggtaatt ccggtattta tgcctcctcc catcgttccc ctgccgcagc
     4741 gcctggccat catccctgtt gacgggtcgt ctgacttcga aatcagctca tggctcgatc
     4801 tgaccctgaa tttcctgcgc acacgccatc cagacttggt gctggtggaa cgagaggatc
     4861 tgaaggcggt gctgaatgag atgggcattc aatacggcgg tcatgtcgat gacgagacgt
     4921 cgatccatat cgggaggctt gtcggcgcgg acagtttgct gacctatcgg atgatcgtgc
     4981 ccgaggacat gccgatgtcg gcggcgttcg aactccgcct tttcaaggtt gaaagcggca
     5041 ccaccctgtt ccgccaaacg gtttcggcaa gtcaggtatc gccgggagcg gtcgtgacca
     5101 gagcccaggg atccgccgaa ctggactcgc tcgcccgtcg tcccgtgctc caagaagccg
     5161 ccgcctatgg cttggctgcg ttcacggctg ccttcggcga taatccgtt
//
LOCUS       DATNDD010000016         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3141913, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000016 DATNDD010000000
VERSION     DATNDD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141913"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VN647_00405"
     CDS             complement(<1..451)
                     /locus_tag="VN647_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95512.1"
                     /translation="MIRCDRRFYRLRAYRLLVCAGFISMLGGCVNDKEVLQKSQGHYQ
                     EGVASLPGDRQTAFVSFQKSVQLDPDNKEARYALGHVYALQGKLSYAEEQFRAAIKID
                     ETYSEAHTYLGQVLANQDRWDEAIQSYRRALANPLYPTPDLARFHLGR"
     gene            complement(448..1362)
                     /locus_tag="VN647_00410"
     CDS             complement(448..1362)
                     /locus_tag="VN647_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWG95513.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEGIEILGGSATYFSTAASFFTSVA
                     LIAVVGEDFPQQHIAFLKSRGIDLTGLERRSGATFRWKGEYTHQLNEAHTLDTQLNVF
                     ETFRPQIPEAYRAPDVLFLGNINPELQLDVLNKVKRPELVACDTMNFWINGQREALWK
                     VLEKVDILIINDGEARALGQDSNLVKVAKLVLSRGPKHLIVKRGEYGVLMFNEKHIFG
                     APAFPLEDVRDPTGAGDTFAGGFLGYLAATGNRSPEAMRQAIIFGSVMASFTVESFSL
                     DRLRILDYKEIQARFAEFKRLTHFEDVQ"
     gene            complement(1365..2264)
                     /gene="mtnP"
                     /locus_tag="VN647_00415"
     CDS             complement(1365..2264)
                     /gene="mtnP"
                     /locus_tag="VN647_00415"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWG95514.1"
                     /translation="MKRKRQDERVTVGVIGGSGLYDIEGLTSTRSIRVRTPFGAPSDA
                     ITVGSLEGIRVAFLSRHGRGHVLNPGGINYRANIFALKSLGVSHVISISAVGSMKESI
                     HPGDVVVPDQFIDLTKRRVSTFFDEGVVAHVAFGEPICAELGQTLLAAGEKVGAKLHR
                     GGTYLCMEGPQFSTKAESRLYRQWGVDVIGMTNMPEAKLAREAELCYATLALVTDYDC
                     WHETEEAVTVEAVLGTLHRNVALAKQILRSAMPSFADPIDCSCHRALDNAILTAPKRI
                     PPAVRKKLAVLIDRALTPKKGVR"
     gene            complement(2291..>2827)
                     /locus_tag="VN647_00420"
     CDS             complement(2291..>2827)
                     /locus_tag="VN647_00420"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HWG95515.1"
                     /translation="TATRLHPNDESKVMRALEVYRLSGRPLSTMQAEHGFHETPFSAL
                     LIGLERRKETLYRRIEERIDWQLTHGMVEETRLLLDQGYGRELGSMKGLGYRQVGAYL
                     ANECDYAEMVHRFKRDTRRFAKRQMTWFRSEGGVAWLSIEDNEPYERTAERVIAHIEQ
                     FMRALGQQKQPAALQQAF"
BASE COUNT          635 a          849 c          694 g          649 t
ORIGIN      
        1 cacgaccgag atgaaatcga gctaagtccg gcgtcggata gaggggattc gccagggccc
       61 ggcgatagga ttggattgcc tcgtcccacc gatcctgatt ggccaaaact tgtcccaaat
      121 acgtatgcgc ttcggagtaa gtttcatcga tcttgatcgc cgcgcggaat tgctcttctg
      181 cgtaggataa cttaccctgc agcgcgtaca catgtcccag tgcgtaccgc gcctccttat
      241 tgtccggatc caactgaaca gacttttgga atgagacgaa agccgtttga cgatcccccg
      301 gaaggctggc gacaccctct tgataatgcc cttgcgattt ttgcagcact tctttgtcgt
      361 ttacacatcc gcccaacata cttatgaatc ctgcacagac cagcagacga tacgctcgca
      421 gccgatagaa ccgccgatcg catctgatca ttgaacatcc tcaaaatgcg tgagccgctt
      481 aaactcggcg aaccgagcct gaatctcttt gtaatccagg atgcgcaatc ggtcaagact
      541 aaaggattct acggtaaatg acgccatcac gctgccgaag atgatggctt gtctcatggc
      601 ctccggagac cggtttcccg tcgccgccaa gtagcccagg aatccgccgg cgaaggtatc
      661 tccggcaccg gtcggatcgc gtacgtcttc gagcggaaaa gccggcgcgc cgaaaatgtg
      721 tttttcattg aacatgagca caccatactc gccccgcttg acgatgagat gcttgggccc
      781 tcgtgaaagc acgagcttcg cgactttcac cagattggaa tcttggccca gtgcgcgcgc
      841 ctcgccgtcg ttgatgatca gaatatccac cttctccaac accttccaga gcgcctcgcg
      901 ctgaccgttg atccagaagt tcatcgtatc gcaggccacc aactcgggac gtttcacttt
      961 gttcagcaca tcgagttgca gctccggatt gatattcccc aggaacaaca catccggggc
     1021 acgataggct tcaggaatct ggggacgaaa cgtctcgaac acgttgagct gggtatccaa
     1081 ggtatgggct tcgttcaact gatgcgtgta ttcacccttc caacgaaagg tcgcccctga
     1141 acgccgttcc aggccggtga gatcgatccc tcggcttttg agaaacgcga tatgctgctg
     1201 ggggaagtct tctccaacca cggcaatgag cgccaccgag gtaaaaaagc tggccgctgt
     1261 tgagaaatag gtagccgacc ctccaagaat ctcgattccc tcgccgaaag gggttttgac
     1321 tgtatcaagc gcgaccgatc ccacgactaa cagtttcccc atggctaacg aactcctttc
     1381 tttggtgtca gagcacggtc tatgagcacg gcgagcttct tccgaacagc tggagggatt
     1441 cgtttcggcg cagtcagaat ggcattgtcc aatgcccgat gacaggagca atcaatcgga
     1501 tcggcaaagg acggcatggc ggagcggagt atttgtttag ccaaagccac attgcgatga
     1561 agcgtgccca acaccgcctc caccgtgacc gcctcttccg tctcatgcca gcagtcataa
     1621 tcggtcacca gcgccaaggt cgcataacag agctctgcct cacgggccag ttttgcttcc
     1681 ggcatattgg tcatcccgat cacatcgacg ccccattgcc gatagagccg tgattccgct
     1741 ttcgtcgaaa actgcggccc ttccatgcag agataggtcc caccgcgatg caacttggcg
     1801 ccgacctttt ctccagccgc caggagagtc tgtcccagtt cggcacaaat cggctcgccg
     1861 aaggccacat gcgccaccac tccctcgtca aaaaatgtcg agacgcgccg cttcgtgaga
     1921 tcgatgaact ggtccggcac aacgacgtcg cccgggtgaa ttgattcctt catgctgcct
     1981 accgcgctga tcgaaatcac gtgagacacc cccagggact tgagcgcgaa aatgttcgcg
     2041 cggtagttga tcccgcccgg attcaacaca tgcccccgcc catgccgtga aagaaatgcg
     2101 actcgaattc cttccagcga acccacggta atggcatcgg aaggagcccc gaaaggcgtc
     2161 cggactcgga ttgaccgagt agaggtgagc ccttcaatgt catacagacc gcttcctccg
     2221 atcactccga cggtcacccg ctcatcctgt ctttttcttt tcatagagat ccttttctta
     2281 tttcttattc ttaaaaggct tgttgtaacg ctgcaggctg tttctgttgt ccaagagctc
     2341 tcataaattg ctcgatatgt gcgatcactc gttctgccgt ccgctcatac ggctcgttgt
     2401 cttcgatcga cagccacgca actccccctt cacttcgaaa ccaggtcatc tgccgtttcg
     2461 caaaacgcct cgtgtctcgc ttaaaccgat gcaccatttc ggcataatcg cactcattcg
     2521 ccaaatacgc tccgacttgg cgatagccga gacctttcat cgaaccgagc tcgcgcccat
     2581 atccctgatc gagcaataat cgagtctctt ctaccattcc atgagtcagc tgccaatcga
     2641 ttcgttcttc aattcttcga tagagggtct ctttccgtcg ctcaagacct atcagaagag
     2701 ccgaaaacgg agtctcgtga aatccgtgtt cggcttgcat ggtcgacagt gggcgccctg
     2761 atagccgata gacttccaat gcgcgcatga ctttggattc atcattaggg tgcaaccgtg
     2821 ttgccgt
//
LOCUS       DATNDD010000017         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2561984, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000017 DATNDD010000000
VERSION     DATNDD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561984"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..317)
                     /locus_tag="VN647_00425"
     CDS             complement(39..317)
                     /locus_tag="VN647_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWG95516.1"
                     /translation="MTKAQIIEKVSEQVTTLTKRQAEVVVNTIFDSVRDSLKNGDKTE
                     IRGFGSFRLRARRMKEGRNPKTGETVAVPAKRVPFFKAGKELKELLNQ"
     gene            complement(366..1268)
                     /gene="sppA"
                     /locus_tag="VN647_00430"
     CDS             complement(366..1268)
                     /gene="sppA"
                     /locus_tag="VN647_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWG95517.1"
                     /translation="MPDDVTQTERPQKRRLFRKVFWLFAVGVGVLILMNLFFPDLDLS
                     TDDRIALIRIEGVILDSQTTIGELKRFSENPSVKAIVIRIDSPGGGVVPSQEIYDAVK
                     RVRSKNNKAVIASMGSVAASGGYYIAAATDRIVANPGTLTGSIGVIMETANVEGLLQK
                     IGVEGIVIKSGKYKDVGSPLRKMSSDERGLLQAVMDDVHKQFIEAVAEGRSLELRVAQ
                     ALADGRIFTGRQAKEAKLVDELGDLEDAIQLAAEVVGIEGEPKVVEPRRRFSLREILD
                     SKLSMMFPKLDMQPGVSLKYLMAF"
     gene            complement(1302..3017)
                     /locus_tag="VN647_00435"
     CDS             complement(1302..3017)
                     /locus_tag="VN647_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWG95518.1"
                     /translation="MGTVSNSSDAQLDRNALAALYEETFRNLEEGTITEGRVVALTKD
                     KIIVDIGYKSEGMIPSDQFSSEELHNLKIGDRLQVYIEECEDADGNLVLSKEKADKMK
                     IWEELEKLYKEEKSIEGKIVSRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGKTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDKKRQTTLANLKEGQLIQGTVKNITDYGSFID
                     LGGIDGLLHITDMSWGRVGHPSELFTVGDKVEVTVLKYDRETGRISLGLKQKSADPWT
                     NVAGKYPIGTRARGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEIRHPSRVVAVGD
                     QVEAAVLNVDPASRKISLGMKQTAPNPWDMIEGKYPIGTRIEGKVKSLTDFGAFVGLE
                     EGIDGLIHISDMSWTKHIKHPSELFKKGQKVEAVVLRIDKEKERLSLGYKQLARDPWD
                     EAIPARYHVGDSVTGKVSKVADFGIFIELEGGVEGLIHVSESGLEPSAKLEEKFKLQD
                     DVTAKIIKVDREERKIALSLRDHQLDWERKQVDDYHSAQGALDQSLGRAAKQSRKRTQ
                     TDDQS"
     gene            complement(3073..3765)
                     /locus_tag="VN647_00440"
     CDS             complement(3073..3765)
                     /locus_tag="VN647_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWG95519.1"
                     /translation="MSGVVYGILWVLVRIVGWVCFRYRVEGQVPQTGGVLIAANHASY
                     LDIPLLGCGMRRRAWYLGRNDLFPIPVLNGILRALGWIPVRLGRLDREAFGKAINLIR
                     AGNAVVIFPEGGRSHDGHLRPPKAGLGVIVSQTGCPVVPAYLKGTFDVLPMGARWPRL
                     RQITVRFGDPIQFETGKQKERAETKQFYQQVSRTVIEHIAALGQVPVPKGRDDQAPET
                     PNRPTAGAHNAE"
     gene            complement(3762..4421)
                     /gene="cmk"
                     /locus_tag="VN647_00445"
     CDS             complement(3762..4421)
                     /gene="cmk"
                     /locus_tag="VN647_00445"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWG95520.1"
                     /translation="MIIAIDGPAGVGKSTVAKLLAARLGYLYLDTGALYRAIGWKTLQ
                     SRIRPTDHEQVATLLPTTSIHMQFHHDTMQVLVDGIDVTGELRTPEVTATASIVSAIP
                     AVREWLLPIQRQIGQRGSVVAEGRDIGTKVFPAAPFKFFLEADADVRVARRYSELVGA
                     GRGGTVETTFRDLSDRDRRDRTRSVDPLVPAGDARLIDTSALSPDQVVEQMIVAVSTG
                     S"
     gene            complement(4467..>4859)
                     /locus_tag="VN647_00450"
     CDS             complement(4467..>4859)
                     /locus_tag="VN647_00450"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWG95521.1"
                     /translation="LIPKTIDEFPVLCVAAAVADGDTVISGAGELRVKESDRIASMSR
                     ELKMMGALLEEQPDGMIIRGLGHGGENGRLKAAGKVESHGDHRVAMSLAIGGLTAEES
                     MTIADTSCVDTSFPNFEKALVDLLGRPA"
BASE COUNT         1059 a         1503 c         1123 g         1174 t
ORIGIN      
        1 tgatgccgac atcggttcct ttttcaacca gacgatggct attgattgag caattccttc
       61 agctctttac cggctttaaa aaacggcacc cgcttggccg gtacggcaac cgtctctccg
      121 gtctttggat tccgcccttc cttcatccga cgagcccgca gtcgaaagct cccgaagcct
      181 cgaatttcag ttttatcgcc gtttttcagc gaatccctga cactatcaaa aatcgtgttg
      241 acgaccactt ctgcctgtcg ctttgtcaac gtggtgactt gctcggaaac cttttcaatg
      301 atctgcgcct tggtcatatc cgctctccct tcgctgcaat aaccaccaca ccttcattga
      361 cacagctaga aggccataag atatttcaag ctcaccccag gttgcatatc caatttggga
      421 aacatcatac tgagcttgga gtccagaatt tcacgaaggg aaaagcgacg gcgtggctcg
      481 acgacctttg gttctccttc gatccccacc acctccgcag ccaattgaat ggcatcttcc
      541 aagtcgccga gttcgtcgac caacttcgct tccttggcct ggcgtccggt aaaaattcga
      601 ccatcggcca acgcttgagc aacccgaagt tcgagcgaac ggccttccgc cacggcttcg
      661 ataaactgct tgtggacgtc atccatcacg gcttgcaaca aacccctttc gtccgagctc
      721 atcttccgga gtggagaacc cacatcctta tatttcccgc tcttaataac gatcccttcc
      781 acaccgatct tctggagtaa tccttcgacg ttggccgttt ccatgatgac tccgatgcta
      841 cctgtcagag tcccagggtt ggccacgatc cgatccgtcg cagcagcgat gtaataccct
      901 ccggatgcgg caacacttcc catcgaggcg ataaccgcct tgttgttctt gcttctgacc
      961 cgcttgactg catcgtaaat ctcttgggat ggtacgacgc cgcccccggg actatcgatt
     1021 cgtatgacga tggccttgac agaaggattc tcgctgaatc gcttcagctc tccaatggtc
     1081 gtctgagaat ccaaaatgac tccttcgatt cgaatcagag cgattcggtc gtcggttgac
     1141 agatcgagat cgggaaaaaa gagattcatc agaatcaaaa cccccacccc caccgcaaac
     1201 agccaaaaga ctttacggaa cagacgacgc ttttgagggc gctcggtctg ggttacatca
     1261 tccggcattg cacatgacct gtctcgcgat gcgaaaccaa cctaactttg atcgtccgtc
     1321 tgcgttcgtt tccggctctg cttagccgcc cgaccgagac tctggtccag tgcaccttgt
     1381 gcagagtgat agtcatcaac ctgcttgcgc tcccagtcca attgatggtc gcgaagactg
     1441 agtgctatct tgcgttcctc tcgatcgact ttgattatct tcgccgtgac gtcgtcctgc
     1501 aacttaaact tttcttccag cttcgcggaa ggctcaagac cggattcgct gacatgaatg
     1561 agaccttcca ctccgccctc caattcgatg aagattccaa aatcggcgac tttggacacc
     1621 ttgccggtca ccgaatcgcc gacgtgatat cgcgcgggaa tcgcctcatc ccagggatca
     1681 cgagccaact gtttgtatcc caacgagagc cgctctttct ctttgtcgat gcgtaacacg
     1741 acagcttcca ctttttgccc ttttttgaac agttcagaag gatgcttgat atgcttcgtc
     1801 caggacatgt ccgaaatatg gatgagcccg tcaatccctt cttcgagtcc gacaaaggcg
     1861 ccgaaatcgg tcaggctctt cacttttcct tcgatacggg tgccgatcgg atatttgccc
     1921 tcgatcatat cccaggggtt tggggccgtc tgtttcatgc ccaatgagat cttgcggctc
     1981 gcggggtcta cgttgagcac cgcggcttcc acttgatcac cgaccgccac gactctcgat
     2041 ggatgtcgga tttcgtgcgt ccacgacatt tccgaaacat gcaccagtcc ctcgactccc
     2101 ggctcaagtt ccacgaacgc cccgtaatcg gtcaggctga cgacacgacc acgggccctg
     2161 gtcccgatag gatacttgcc tgcgacattg gtccagggat ccgcactctt ctgcttgagc
     2221 cccagagaaa tacgacccgt ttcacgatca tatttcaaca ccgtcacttc aaccttgtcg
     2281 cccaccgtaa acagttccga tggatgtccg actcgacccc aggacatatc ggtaatatgc
     2341 agcaacccat caataccgcc gaggtcgata aacgacccgt aatcggtgat gttcttgacg
     2401 gtaccctgga tgagctggcc ttctttgagg ttcgccagtg tcgtttgccg ttttttatcc
     2461 ctggtttctt ccaacaacac gcggcgtgac acgaccacat tgcctcgcct gtggttgatc
     2521 ttgatgattt tgaggggaaa ggtctttcca acaagcccat ccagatcacg aaccggatga
     2581 agatctatct gcgagccggg caagaatgct ttgacgccga tatcgaccat catgccgcct
     2641 ttgatgcgtg agacaatctt gccttcgata ctcttctctt ctttgtagag tttttcgagt
     2701 tcttcccaaa tcttcatctt gtccgctttt tccttggaaa gaacgagatt gccgtctgca
     2761 tcctcacatt cttcgatata cacttggagc cgatcgccga tcttgagatt gtgaagttcc
     2821 tcggaagaga actgatcgct cgggatcatg ccctctgatt tataaccgat atcaacgatg
     2881 attttgtcct tggtcagggc caccacacgg ccttctgtaa tcgtgccctc ttccaagtta
     2941 cggaaggttt cttcgtacaa cgccgccaat gcattccggt ctaactgagc gtcactgctg
     3001 ttggataccg tacccatatg aacgtcctac tcttccgact ctcgtcgggt gctgatggtg
     3061 aagtcgccaa gctcactcag cattgtgagc accggcggtc ggcctgttcg gtgtttcagg
     3121 agcctgatca tctctcccct ttggaacggg gacttgacct aaggctgcaa tatgctcaat
     3181 caccgtgcgg ctgacctgtt gatagaactg tttcgtttcc gccctctctt tttgtttccc
     3241 cgtttcgaac tgaataggat caccgaatcg cactgtaatc tgacgcaacc gaggccagcg
     3301 tgcccccatg gggagtacat cgaaggtccc cttcagatat gccggaacga ccggacaccc
     3361 cgtctgcgat acaatcaccc cgagcccagc tttgggcggc cggagatggc catcgtggct
     3421 gcgtccacct tccgggaata tgaccacggc attacctgct cgaatcaggt tgatcgcctt
     3481 tccaaacgcc tctcggtcga ggcgccccaa cctcaccggt atccagccca atgcccgcaa
     3541 aattccgttc aataccggaa tcggaaacaa gtcgttccgt cccaagtacc aggctcttcg
     3601 acgcatcccg cagccgagca gaggaatatc gagatagctg gcatgatttg cggcgatcag
     3661 cacccccccg gtctgaggaa cctgaccttc gacacgatac cgaaagcaaa cccatccaac
     3721 gattcgtacc aaaacccaca aaattccata gactaccccg ctcacgaccc ggtcgacaca
     3781 gccacgatca tctgctccac cacctggtca gggctgagag cagaggtatc gatgagtcgc
     3841 gcgtcacccg ctggaaccaa tgggtcgacc gaacgggttc gatcacgccg atcccggtcc
     3901 gacagatccc tgaacgttgt ttcaaccgtc ccacctcgac ccgcaccgac cagctcactg
     3961 taccgccgtg caactcgtac gtctgcgtcc gcctccaaaa agaatttgaa tggtgctgcg
     4021 ggaaagacct tggtaccgat gtcccgtccc tctgcgacga ccgatcctct ctggccgatc
     4081 tgacgttgga tcggcaacag ccattcgcgg actgccggaa tagcggacac gatggaggct
     4141 gttgcggtaa cttctggcgt acgaagttct ccggtaacgt cgatgccatc gaccaagacc
     4201 tgcatcgtgt catggtgaaa ctgcatatga atcgaggtag tcggcaataa tgtggccact
     4261 tgctcatgat cagtcggacg tatccttgat tgcaaggttt tccatccgat ggctcggtac
     4321 aatgcccctg tatcaagata caggtagcca aggcgagccg ccagtaattt ggccactgta
     4381 ctcttgccaa ctccggccgg tccatcaatc gcaataatca ctcagcgcct ctcaattgcg
     4441 aactgtccca tccgacacac ctgctttcac gcaggccgcc ccaacagatc gacgagtgcc
     4501 ttctcaaaat ttgggaatga cgtatccacg caactcgtat cggcaatcgt catactctcc
     4561 tcggccgtaa ggccaccgat ggcaagagac atggccacgc gatgatctcc atggctctcg
     4621 accttgccgg cggctttcaa ccggccgttc tctcctccat gacctaagcc gcggatgatc
     4681 atcccgtccg gctgttcctc gagcagagct cccatcatct tcaactcgcg gctcatggag
     4741 gcaatccggt cactctcttt gacccgaagc tcccctgctc cggaaatcac agtatcccca
     4801 tccgccacag ccgcagcaac gcacagcacg ggaaattcat cgatcgtttt gggaatgag
//
LOCUS       DATNDD010000018         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1414458, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000018 DATNDD010000000
VERSION     DATNDD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414458"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VN647_00455"
     CDS             <1..555
                     /locus_tag="VN647_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95522.1"
                     /translation="LIVLFLLTLVVVVVFPRFSLDEDLSSTGRKLVGVLRTFQELAAT
                     RQQPLKLYLDMDRGTYWMMTVEDKEERLPLDPAWKLPRSLPESIRLTEVSVGQDKHFS
                     GRVDISFFANGRIESVTMYLMDEKNTLLGLAVDSLTGGIRVSDERFEPPPNRIIPDRV
                     RLLLKPNKQAGAGGLPRGLFPTQQ"
     gene            562..999
                     /locus_tag="VN647_00460"
     CDS             562..999
                     /locus_tag="VN647_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWG95523.1"
                     /translation="MTGDVHRTKSDEDGFTLLEVLLAIALLAIALPILLGLRNFDLEL
                     QERASELTAATLLAQEKLLETELVGQYAIGESTGEFPNIPLGTQLTMEAVPRAVGYKW
                     KRTIAPTPLELIREIRIKVSWFRGGLEEAVEVSTYVFAGRATF"
     gene            971..1594
                     /locus_tag="VN647_00465"
     CDS             971..1594
                     /locus_tag="VN647_00465"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HWG95524.1"
                     /translation="MSSPAVPRSKSQAGFTLVEILVAVALLGVIGAMVFGSLVMTMNA
                     VDAGREHAAKEQAIRRILRLMTEEISFSKRNTMYPWVGTNATLDGQPADILAFLAMSH
                     ELSTSAAKESETVRVVYTRERDRLIRFVRRNLYTLTDTNETLDQMELADRVQAFNIRY
                     YDDQNRIWIDEWPAASKLPKALLIEVTFQYPDAAPWTVREWVAIGAS"
     gene            1570..2475
                     /gene="gspN"
                     /locus_tag="VN647_00470"
     CDS             1570..2475
                     /gene="gspN"
                     /locus_tag="VN647_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HWG95525.1"
                     /translation="MGGDRRIMTIKWPEAWREILAWTVAGVTILALCLMATFPYGMLQ
                     ARVVAELSRATGMEIRVVDWTVGLPLGLEWRNVTLSQPNWTPLQLALLQAKLGVMKAL
                     GGTLGLDVVAKLNETASPPGLAKGTVTASSLSLASPVTIKGQVQHVDLSKVLRRYVTH
                     GTLSGDFSHRIDSSQTTATTMKGEGMWTAEATDLVVDQIPLGNGRILSLTFSKVSAGL
                     ACRDLRCDVTQLKGDGIDGSFTGEGYITIQQPLQQSQVNLTVTVIPGPGFASKAGTLG
                     LPSPPPGTAMTVKIVGTLAQARIAL"
     gene            2594..>2853
                     /locus_tag="VN647_00475"
     CDS             2594..>2853
                     /locus_tag="VN647_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95526.1"
                     /translation="MAIVNECVGLDIGQTGLKAVRFRRRLSGRETIDYFQHPMPFSRP
                     EDLEPARRVKSLRGFLWQNGLYGTDRLVTAIPCQDLFVRTLS"
BASE COUNT          638 a          807 c          826 g          582 t
ORIGIN      
        1 ctgatcgtcc tgtttctcct gaccttggtc gtggtggtcg tgttccctcg attcagcctc
       61 gacgaggacc tgagctccac cggacggaaa ctcgtcggtg tccttcgaac atttcaagaa
      121 ctggcggcga caagacaaca acccctgaaa ctctacctcg atatggatcg ggggacctac
      181 tggatgatga cggtggagga caaagaagaa cgacttccgc tcgatccggc atggaagctg
      241 cctcgttcgc tgcctgaatc aattcgattg acggaagtct ccgtcggaca agacaaacat
      301 ttttccgggc gagtcgacat atccttcttc gccaacgggc ggattgagtc ggtcacgatg
      361 tacctgatgg atgagaagaa tactctcttg ggactggcgg tcgattcatt gacgggaggg
      421 attcgagtca gcgacgaacg gttcgaacct cctccgaacc ggatcatccc cgaccgagtc
      481 cgactcctct tgaagccgaa caaacaagca ggagcaggag ggttgccgag agggttgttt
      541 ccaactcaac agtagctcgc gatgactgga gatgtacacc gaaccaaatc cgacgaagac
      601 ggattcacgc tgctcgaagt actgctggcc atcgccttgc tggccatcgc gctcccgatt
      661 ctccttggtc tcagaaattt cgatctggag ctccaggaga gagcgtccga gctgacggcc
      721 gccacgctgc tggctcaaga gaaactactg gaaacagaac tcgtaggaca atatgccatc
      781 ggggaaagta ccggagagtt tccgaacatc ccgcttggaa cacaactgac catggaggcg
      841 gtcccgagag cggtcgggta caaatggaag cggacgatcg cacccactcc gctggagctg
      901 attcgagaga ttcggatcaa ggtctcgtgg tttcggggcg gcctggaaga agcggtggag
      961 gtgagcacgt atgtcttcgc cggccgtgcc acgttctaaa tcccaagcgg gctttacgct
     1021 cgtcgagatc ttggtcgccg tggccttgtt gggcgtgatc ggcgccatgg tctttggttc
     1081 gctcgtcatg acgatgaacg ccgtggatgc cgggcgagag cacgcggcga aggaacaggc
     1141 gatcagaaga attttgcgcc tcatgaccga ggaaatttcc ttcagcaaac gcaataccat
     1201 gtatccctgg gtgggaacga atgcgacgct agacgggcaa ccggccgaca tcctggcgtt
     1261 cctcgcgatg agtcacgaat tgagtacgtc ggccgccaag gaaagcgaga ccgttcgcgt
     1321 ggtctacaca cgtgaacgtg atcggctgat tcggtttgtc cgcagaaatc tctatacgct
     1381 gaccgatacc aatgagacat tggatcaaat ggaactggcc gatcgtgtcc aggccttcaa
     1441 tatccgatat tacgacgacc agaaccggat ctggatcgac gaatggccgg cggcctcaaa
     1501 actccccaag gccttgttga tcgaagtgac gtttcaatat cccgacgccg ccccctggac
     1561 ggtgcgggaa tgggtggcga tcggcgcatc atgaccatta aatggcctga agcatggcga
     1621 gagatcttgg cttggacggt agcgggcgtc accatcttgg cgttgtgcct gatggcgacc
     1681 tttccgtacg gcatgctgca agcgcgagtg gttgcggaac tctcacgagc caccggcatg
     1741 gagatccgag ttgtcgattg gacggtggga cttccattag gccttgaatg gcggaacgtc
     1801 accttgtcgc aaccgaactg gaccccgctt caattggctc tgttgcaggc caagctcgga
     1861 gtcatgaaag cgttgggcgg cacgcttgga ctcgatgtcg tcgcaaagct gaacgagact
     1921 gcttccccgc cgggtctcgc caaaggcacc gtcaccgcct catctctttc cttggcaagc
     1981 cccgtcacga tcaaagggca ggttcagcac gtggaccttt ccaaagtcct ccgccgctac
     2041 gtcacgcacg gcacgctcag cggagatttt tcccatcgga tcgactcgag ccagacgacc
     2101 gccaccacga tgaaggggga aggcatgtgg acggccgagg cgacggatct ggtggtcgat
     2161 cagattcccc ttggaaacgg acgaatcctg tcactcacat tcagcaaggt ctctgcagga
     2221 ctcgcatgcc gcgacctccg atgcgacgtg acgcaactga agggcgacgg catcgacggc
     2281 tcctttacgg gcgaaggata catcaccatt cagcaacccc tgcaacagag ccaagtgaat
     2341 ctcacggtga cggtcatccc cggccctgga tttgcttcga aagccggcac actgggcctc
     2401 ccctcgccgc ctccgggaac agccatgacc gtcaagatcg tcggaacctt ggcacaggcg
     2461 aggattgcat tgtaaagtac ggctggcgtt ttaagttgcg ggttggagat tgcaatttca
     2521 gtgtacctga tacgatgcac tcgtacatga aacccgaaac caaaatcacg aaacacctac
     2581 ggaagtcgga tgcatggcca ttgtaaacga atgtgtcggg ctggatatcg gccaaacggg
     2641 attgaaagcc gtgcgtttcc gccgccgcct gagcgggcgc gaaacgatcg attacttcca
     2701 acatcctatg ccgttttccc gccccgagga tctcgagccg gcacgacgtg tgaagtcgct
     2761 gcgtggattc ctctggcaga acggactcta tggcacagac cggttagtga ccgcgattcc
     2821 ttgccaggac cttttcgtca gaaccctgtc ctt
//
LOCUS       DATNDD010000019         3872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2869465, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000019 DATNDD010000000
VERSION     DATNDD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2869465"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..700
                     /gene="umuD"
                     /locus_tag="VN647_00480"
                     /pseudo
     CDS             381..700
                     /gene="umuD"
                     /locus_tag="VN647_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
     gene            complement(760..1683)
                     /locus_tag="VN647_00485"
     CDS             complement(760..1683)
                     /locus_tag="VN647_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWG95527.1"
                     /translation="MSLLIICFLLTYLTNEPAAAATQYRPAELRILTYNLLHDGPWSG
                     FFENGTHLEERLDMTIQELRRLQPDIIAFQEASDSRRHGNVPQRIADALGYHMVFAPA
                     TERIFGIGRLNRLIISILGFKEGPAILSRYPIVDSEVYDLPRCRYRLDPRILLRAEID
                     IPAGPVQVFSAHTSKGDECQLQRVGELFQAHRGTGQAILMGDLNTGEQSSVLAGWQKE
                     PGFIDVFRLANPGVSGGTVWQDIHVERSTADRRVDFIFLLGGRKVEGPIVRSSRLAFD
                     RPGRLPSGAVLWPSDHRGVLAEIEVTPFQSD"
     gene            1848..2132
                     /locus_tag="VN647_00490"
     CDS             1848..2132
                     /locus_tag="VN647_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95528.1"
                     /translation="MLLTVSGSPVLAADITPPSQSPPPKDPSTEKSVPDSPPSTRDPG
                     MVKQPQTAPHPDSVVTPPVIDPKMAVDPEAKTEEERHPTLPPEQTEPPKQ"
     gene            2149..2421
                     /locus_tag="VN647_00495"
     CDS             2149..2421
                     /locus_tag="VN647_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95529.1"
                     /translation="MPIIPDAKKDIKDLIVAGCRVQLFFRAIERGQWTVQGTVRCGVD
                     DHAAEQSFCTASYPTREEAEQEALKQAADLLGNNVDRNTSRIINWN"
     gene            2440..3006
                     /locus_tag="VN647_00500"
     CDS             2440..3006
                     /locus_tag="VN647_00500"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWG95530.1"
                     /translation="MPMIGQAIPEGTYQTYHQNEIQSVTLQSYRGRWMVLVFYPGDFT
                     FICPTELEDLGMLYPEFQKLNAEVLGISTDSVYVHKAWRDTSPSVKDLPFPLAAAPGG
                     QLAKKLGLYLPQDGVALRGSFVFDPDGTLCAYEVHNNSIGRNMKELLRKIQAAVFVRE
                     NGGIEVCPAGWKPGERTLKPGLDLVGKI"
     gene            3434..3700
                     /locus_tag="VN647_00505"
     CDS             3434..3700
                     /locus_tag="VN647_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWG95531.1"
                     /translation="MPNITLLVSPSCGACPSAKSLWKQLRVKYSFSYREVDITTKDGQ
                     ELADRHSVRAVPATIIDGRLTFVGVPSRESAEKAIQLKIKQREE"
     gene            3708..3860
                     /locus_tag="VN647_00510"
     CDS             3708..3860
                     /locus_tag="VN647_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95532.1"
                     /translation="MDEDDIELPVLPKLDRPPPDCPICGDPMSFIDGDWACVDCNGEL
                     LGPETG"
BASE COUNT          926 a         1046 c         1061 g          839 t
ORIGIN      
        1 cctctcttat cacaagcacg acgggccgag gagaggtttg tcaacacact gctagacaga
       61 ccaatcgtta ttgagcaagc ctcttagctt tccggtgcac gaatgactta agatgttcgt
      121 gcaccgaata cgagcgacta agcgatctca caccgctacc gcaacctccc gtaattcgaa
      181 ggacagcata aaaggtaagc atggtgggtt cccctccaag gaacaatgcc ccacaactac
      241 ttgagcagat tgcaatttag gaatagactc gcgcccatga acgtagacac tatttacgca
      301 cccgacctca ccacccgcta taagctgccc atttcttagg gcggctcccc gctggctttc
      361 cttcgcctgc cgatgattat atggaaggca agctcgatat taccaagcac ctggtcaaac
      421 acccggcggc aacattttat gtccgcgtgt ccggcgattc gatgatcgga gcgggaatcc
      481 attccggcga tttgctgtgg tggatcgctc attggaaccc aagaacggca acgtcatcgt
      541 ggccgccctc gatggcgatt tgacggtcaa atgcctctac cagcgcgatg gtgctatctt
      601 cctgctgccg gaaaatcagg cgtaccaggc cattgagatc caggacggcc agtcattcga
      661 gatctgggga gtagccacca atgttattca cccgctgtag tcatgcctcc cgcctccccc
      721 cggggtatgc cctcctcgat tgcaataatt ctacgcaagc tagtcgcttt ggaaaggagt
      781 aacttcgatt tccgcaagta cgccgcgatg atcagacggc catagcacgg cgccgctcgg
      841 aagacgaccc ggccggtcaa atgccaggcg acttgaacgc acgatcggac cttcgacctt
      901 tctcccaccc agcaagaaaa taaaatcgac gcggcgatcg gcggtggacc gttcgacgtg
      961 gatgtcttgc cagacggtcc ctccggatac tcctgggttg gcgagccgaa atacatcgat
     1021 gaatcccggt tccttctgcc atccggcgag gaccgaagat tgctcgccgg tattcaagtc
     1081 gcccatcagg atggcttgtc ccgttcctcg atgcgcttgg aacagttcgc cgactcgctg
     1141 tagttggcat tcgtcgcctt tcgaggtatg ggccgaaaag acctggaccg gcccggcggg
     1201 tatgtcgatc tcggcacgaa gcagaatgcg agggtcgagc cgatatcggc agcggggcaa
     1261 gtcatagacc tccgagtcaa cgatcggata ccggctcaag atggcgggtc cctctttgaa
     1321 ccctaagatc gagatgatca gtcgattcaa gcggccgatg ccgaagatac gctcggtcgc
     1381 gggggcgaac accatgtgat acccaagcgc atcggcgatc cgttgcggta cgttgccatg
     1441 cctccggctg tccgacgctt cctgaaaggc aatgatatca ggctgtaaac gccgcagttc
     1501 ttgaatggtc atgtcgagcc gctcttcgag gtgcgtgccg ttctcgaaga atcccgacca
     1561 tggcccgtcg tgcaggaggt tgtaagtcaa gatacggagt tcggcaggtc ggtattgagt
     1621 tgctgctgcc gctggttcat tcgtcaggta ggtcagcaga aaacaaatga tcaacaacga
     1681 catgaggatc gatcgcattg taggacaagc aaaatggtca taaggatcta acaagtgagc
     1741 gtttcacgaa gaacctacct attcatagac atcaggggta tctccgagta ggcagaatgc
     1801 atgtcatccg atatcgttaa atcatgcggc gatggctctt cattataatg ctgctcactg
     1861 taagtggttc acctgtgctg gcggccgata tcactccgcc gtcccaatcg cctccgccaa
     1921 aagatccctc gaccgagaaa tcagttccgg acagtcctcc cagtactcgt gatcctggta
     1981 tggtgaaaca gccccagacg gctcctcacc cggattctgt cgtgacgccg cctgtgatcg
     2041 atcccaaaat ggccgttgat ccggaggcaa aaacagaaga ggaacggcat ccgacacttc
     2101 cgcctgaaca gaccgaaccg ccgaaacaat aacggtcgag agacggcaat gcccatcatt
     2161 cctgatgcca agaaagatat caaggacctg atagtagccg gctgccgcgt gcagctcttc
     2221 ttccgtgcaa ttgagcgagg acaatggacc gtccagggca ccgtgagatg cggagttgac
     2281 gaccatgccg ccgaacaatc tttttgtaca gcttcttacc ctacgcgcga ggaagctgaa
     2341 caggaagctc tgaaacaagc ggctgatctg ttgggaaaca acgtggatag aaataccagc
     2401 cgaatcatca actggaacta agaagaggag cagggctgca tgccgatgat cggacaagcg
     2461 attccggaag ggacgtatca aacctatcat caaaatgaga ttcaatcggt gacgctacag
     2521 agctatcgcg gccgttggat ggttctggtg ttctatccgg gtgacttcac cttcatctgt
     2581 ccgacggaat tggaagatct agggatgctg tatccggaat ttcagaagtt gaatgctgaa
     2641 gtgctgggca tcagcaccga ttccgtctac gttcataaag cgtggcgtga cacttctccg
     2701 tcagttaagg atctgccttt tccattggcg gctgccccag gagggcagtt ggccaagaag
     2761 cttggcttgt atctgccgca ggatggcgtg gctctgcgtg gcagttttgt gttcgacccg
     2821 gacggaacac tttgcgcgta tgaagtccac aacaattcca ttggacgtaa catgaaagag
     2881 ttgttacgca agatacaagc ggctgtcttc gttcgtgaga atgggggaat cgaagtctgt
     2941 cccgcaggat ggaagcctgg cgaaaggacg ttgaaaccag gcctcgacct ggtcgggaag
     3001 atttaatcgc gatgaagaaa ttagaggtga tcttatggac tccttgtcaa aatgcggctg
     3061 cggacgatta tccggtttcc tttgttgcgt gatgctgatg gcatgtatgg gttgcatacc
     3121 gaaggaaact ctaaacgctc tatcccagaa taccggagag ccgggaagca ccatccctcg
     3181 atcccacgct ccctattatg ctaatagtgc cgggaacatt gtcgccccgc cgggaaatcc
     3241 agctggaaat cccgccggct ctccgcccat catcaatggc acggaaaatt cgtccgacgg
     3301 atggccgtca gggagataaa ttctctcgcg attatccgat ccaacggcgt gtcattgggc
     3361 aagcgctcct tgcgccggct tttcagccga tgttaagatc gaataggaaa ggctgagggg
     3421 gacttgtccg acgatgccga acatcacctt gcttgtgtcg cccagttgcg gagcctgtcc
     3481 gtcagccaag agtctgtgga agcagctgag agttaaatac agcttctctt atcgggaagt
     3541 tgatattacg acgaaagacg gccaggaact ggccgatcgg cattctgtcc gtgccgtgcc
     3601 cgcaaccatc atcgacggac ggttgacgtt tgtcggagtg ccgagtcgag agagtgcgga
     3661 aaaggccatt caattgaaaa ttaaacaacg ggaagagtag ggagtccatg gacgaagacg
     3721 atatcgaact accggtcctt cctaaactcg atagacctcc tcccgattgt ccgatctgcg
     3781 gcgaccccat gtctttcatc gacggcgatt gggcctgtgt cgattgcaac ggggagctct
     3841 tggggccgga gaccgggtag cgtacaatga cc
//
LOCUS       DATNDD010000020         6167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_552553, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000020 DATNDD010000000
VERSION     DATNDD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552553"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VN647_00515"
     CDS             <1..252
                     /locus_tag="VN647_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95533.1"
                     /translation="ARFHLGRALAHQGDLQGSIEALEDAASASPPSVPPAMTHLELGR
                     VYYKLGYRTRAREILKKVATWDKGGELATAAAELLTRLK"
     gene            260..1180
                     /locus_tag="VN647_00520"
     CDS             260..1180
                     /locus_tag="VN647_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWG95534.1"
                     /translation="MESVGEFFRQVRETKGLTVDEVASKTRIRTDFVKALEDGNFAKL
                     PDQVFAKGFVRSYARSLGLDEEDAIHRFIQSAGSFYEKQDERERLKVRQIEEDRKRQS
                     NRKAVAIAISIAVLTLIFLLSREQSSVFRRGAPEQGPATKRTTQAAKEVPSSTAREPE
                     RTAEVPKSADMSAGAPKTATEATTRQEPVPPAGTAARSEPETVSTAPSGSDGPLAGIA
                     LNATESSGDGQLVLDLEATELSWVVVQIDNGSPQELLLRPGEKAHWKGQDQFILTLGN
                     AGGVKAELNGKPQKPFGPSGKVARDIVLKR"
     gene            1657..2103
                     /locus_tag="VN647_00525"
     CDS             1657..2103
                     /locus_tag="VN647_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWG95535.1"
                     /translation="MGYLSKFLGVTAAVVFLSVSVVGAEERDPLKPRVPPDQAADAKA
                     LKNPVASSPESIAKGKALYEGKGTCFNCHGKAGDGQGEAGKILNPSPRDFTNCKFHKK
                     RKDGELFWVIKNGSAGTGMVSLIPAAITEEEAWAIINYERSFCKAE"
     gene            2461..4311
                     /locus_tag="VN647_00530"
     CDS             2461..4311
                     /locus_tag="VN647_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95536.1"
                     /translation="MYSLWPWVKCRVGVVTVTLVLGSTGIIAGTATDKSHAGDTALPP
                     GVVATDIEYTGRIAVNEDPVTVKIATPNKNGVMVFDGTAGQRINIGFSGVTLTQFYVS
                     VYRPDGGTVPRHVSAVKNYYATMGERPLSSQTKVHTSMPAYQFSTDSAATISSSEMNG
                     ASVDLEELPVTGTYTIFIDPLSNYTGSFKITVSSELSGEIVPHGSAVTANINRTGQKA
                     RYTFSGTTGQAVSLQLSDVTIRSGYVSIVAPDGSLLGKPISFVSGGGVGGAMIPGQVL
                     PASGTYAVLVESDLSYTGKLKLSLYNAPDLTGTIVIDQGTVTPNLTVPGQRARYTFNG
                     TEGQWVNVGLTGVSIPMTTVAILKPDGSKWESTTVGPSGGSLDPLTALPATGTYTIAV
                     EPVSNYTGSMTIALSSPVTGTIAIDGASVPVNLNKVGQTARYTFNGNAGQWVNLGLTS
                     VSITSSAVTLLTSDGTILASTAVGTAGGGLEDRNPLPTTDTYTILVDPVGSYTGKMTL
                     TLSTEVADSLKLNGAPRPITVSRAGQNGRYTFTGAANQQAMIKATNNKLGSVTVNLYS
                     SNGILLSGITSSAPSFTLNPVTLASADTYTVTINPALTETGSIQLQVSSQ"
     gene            5428..5550
                     /locus_tag="VN647_00535"
     CDS             5428..5550
                     /locus_tag="VN647_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95537.1"
                     /translation="MRKIPIRDFKAEPRFVLDLGTHLHAEFQMAWNSSAEWSHR"
BASE COUNT         1447 a         1582 c         1730 g         1408 t
ORIGIN      
        1 gctcgatttc atctcggtcg tgcactggca caccaaggcg atcttcaggg ttcgatagag
       61 gcgttggaag atgcggcatc ggcgagtcct ccaagtgtcc cgcccgcgat gacccatctt
      121 gagctcggtc gggtgtacta taaattaggt tataggacca gagcccggga gattttgaaa
      181 aaagtggcga cctgggataa gggtggagag ctggcgacgg cggcagcgga acttttgacg
      241 cgattgaagt aggagactta tggagtcggt cggcgaattc ttcagacaag tgcgagagac
      301 caaagggttg accgttgatg aagtggcatc gaagactcgg attcgcaccg atttcgtcaa
      361 ggcgcttgag gacgggaatt tcgccaagct acccgaccaa gtctttgcca aggggtttgt
      421 gcgttcttac gcccgatcgc tgggcttgga tgaagaagat gcaattcacc gctttattca
      481 atcggccggc tccttttatg agaaacagga tgagcgcgaa cgactcaagg tgcggcagat
      541 tgaagaagac cggaagcgtc agtccaatcg gaaagctgtg gcgattgcaa tcagtattgc
      601 cgtgctgacg ctcatcttcc ttctcagtcg agaacaatcg tcggtctttc ggcgtggggc
      661 tcctgagcaa ggcccggcga cgaagcgcac gactcaagcg gcaaaagagg ttccgtcctc
      721 aacggcccgt gagcccgaac gtacggcgga ggttccaaaa tcggccgata tgtctgccgg
      781 agcgccaaag acagcgaccg aagccacgac gcgacaggaa cctgtcccgc ctgcaggtac
      841 agccgctcga tcagagcctg aaacggtttc gacggcccct tccggcagtg atggcccgtt
      901 ggctggaatc gctctgaacg caacggaaag ttccggggat ggtcagctgg tattggactt
      961 ggaagcaaca gagttaagtt gggtcgtcgt gcagattgat aatgggagtc ctcaggagtt
     1021 gttgctacga ccaggtgaaa aagcccattg gaaggggcag gatcaattca tcttgaccct
     1081 tgggaatgcc ggaggagtga aggccgagtt aaacggcaaa cctcaaaagc ccttcggacc
     1141 gagcggaaaa gttgcacggg atattgtatt gaaacgataa tatcgagccc cccaatcgcg
     1201 ttcactcaat ctgcctgtgc ttgctttccg gccataatct cctaacaatc cgatcaagcg
     1261 cctccttgtc ggacccttga ttttagatgg attttcatag cctcatttca agctgctgcc
     1321 gttagtggcc gttatatcgt tggcaatgag gcgtggaggg cgtcagtgag gcgcaaaggt
     1381 gtcacgaata gctgaaaata tggaatgggc ttacggtcaa ccacataaat accgacattt
     1441 tcttgcgtct ttttgagttg gtgttggcat gaggtttgat ctctctttca taacaagaag
     1501 agggacgagc gatggcgaga gggatgtttt cttgacagga attcaagggc attgatatag
     1561 ttcgaccgtt ttgcctgacc ttgaaatcat gaagacgagt cggcggcctg gaatatcacc
     1621 caggctcggt gggtttaaca caaaggagga cgtgcgatgg ggtatctgtc caagtttttg
     1681 ggagtcactg ccgcagttgt gtttctctcg gtctcggttg ttggggccga ggaaagagat
     1741 cctttgaagc cgcgtgtccc gcctgatcaa gcagcggacg caaaagctct gaaaaatccg
     1801 gtcgcgtcca gtccggagag catcgccaaa ggcaaggcgc tctacgaagg gaaaggcact
     1861 tgcttcaatt gtcatggcaa ggccggagac ggccagggtg aagccgggaa gatcctgaac
     1921 ccaagcccac gggatttcac caattgcaag ttccacaaga aacggaagga tggcgaactc
     1981 ttctgggtca ttaagaacgg cagcgccggc acggggatgg tttccttgat ccctgcagcg
     2041 atcacggaag aagaagcttg ggcgatcatc aactatgaac ggagtttctg caaggcagag
     2101 tagttctgaa gcttctagtg cgttgtaaag tggggtgggg aggcaactct ccaccctctt
     2161 tttttgctgt caggcgatcg aataagcacg tttcccattc cgaatgctct tttgtttttc
     2221 tttcctaaca aaaatctata cctccacacc tggaacgatt ggtctccgag ccttggctga
     2281 tctaatcccc ccagttgggg gggggcgtat agataatcgt ctaaagggtg tctatgggga
     2341 ttgcgccacg gacacaaaaa agggtaaagt ccgcgcgtca acccagcgtc gcaaggcaaa
     2401 ctcaaacgag gtgacagtca ccaaaagagc cgtcagattt tataaatagg aggggcgact
     2461 atgtacagcc tatggccgtg ggtgaagtgc cgagtaggag tggtaacagt cacgcttgtg
     2521 cttgggtcaa cgggaattat tgccggtact gccactgaca agagtcacgc cggagatact
     2581 gccctaccgc caggcgtcgt ggcaacagac atcgagtaca ctggtcgcat tgccgtcaac
     2641 gaggatccag tcacggtgaa gatcgccact ccgaataaga acggtgtgat ggtcttcgac
     2701 ggcacagccg gccaacgcat caatatcggc ttcagcggtg tcaccctcac acagttttac
     2761 gtttcagtct atcggcctga tggcgggacc gtacccagac atgtctccgc agtgaagaac
     2821 tattatgcga ctatgggcga gcggccactc tcttcacaga caaaagtgca tacatccatg
     2881 ccagcctacc agttttcaac cgattcggcg gccacgattt cttccagcga gatgaacggc
     2941 gcaagcgttg atcttgagga attgccggtg acggggacgt atacaatttt catcgacccc
     3001 ctcagtaact atacgggaag tttcaagatc acggtctcaa gcgagctcag cggtgaaatt
     3061 gtccctcacg gttcagctgt aacggccaat atcaatcgga ccgggcagaa ggcccgttat
     3121 acattctccg gtaccaccgg ccaagcggtg agtttacaat tgagcgacgt gacgatccga
     3181 agcgggtacg tctctattgt tgctccggat ggaagtttgt taggcaaacc gatctcgttt
     3241 gtctcggggg gtggtgttgg aggtgctatg attccgggac aggttcttcc ggcttccgga
     3301 acctatgccg tcttggtcga atcagatctg agttacaccg gaaaactgaa gctttcgctt
     3361 tataacgccc cagacttaac cggcacgatc gtgattgacc aaggaaccgt gacaccgaac
     3421 ctgaccgtac cgggtcaacg ggcgcgctat acatttaacg ggacggaggg gcaatgggtg
     3481 aacgtcggtc ttaccggggt gtcaattccc atgactactg tggccatact gaaaccagat
     3541 ggcagtaagt gggaatcgac cacagttggt ccaagtggcg gtagtctcga tcccctgacg
     3601 gctctgccgg caaccggaac ttacaccatc gccgtggagc cggttagtaa ctataccggc
     3661 agtatgacga tcgccctttc ttctcccgtc acgggtacaa ttgccatcga tggagcctca
     3721 gtgccggtca atctaaacaa ggtgggccaa acggcgcgct atacgttcaa tggcaacgct
     3781 gggcaatggg tgaatctcgg gcttacgtcc gtgagtatta cgtccagcgc ggtcacgctt
     3841 ttgacatcgg atgggaccat cctcgcttca acggccgtgg gaacggccgg cgggggcctc
     3901 gaagatcgaa accccttgcc gaccaccgac acgtatacca ttctagtcga tccagtcggt
     3961 agctataccg gaaaaatgac cttgaccctc tcgaccgagg ttgcggattc attaaagttg
     4021 aatggagctc ctcggccgat cacggtcagc cgagccggcc agaatgggcg ctatacgttt
     4081 acaggtgctg cgaatcaaca ggccatgatc aaggctacca acaataagct cggcagtgtg
     4141 acggtgaatc tctatagctc aaatggcatt ctgctatccg ggataacgag ttcagcgccg
     4201 agctttaccc tcaaccctgt caccttggct tcagcagaca cgtacacggt caccatcaac
     4261 cctgcgctga ctgagacggg cagtattcaa ctgcaagtga gtagccaata ggaggggagt
     4321 ttctgcggag gcgaattatc gccttgctga gaccgtcaca gattgaaaga gggtggggag
     4381 gcaactcctc accctctttt tttgacgcga agcgacgatg ttcgaatgaa ggtggtcaag
     4441 aggtcaggta cagtgtgaat cgtacggatg ctgcaggagg agagacttga ggtagggcag
     4501 ctgctggggg gcgaagcctt tccctcgatt cttagtctta tgcggctttt aggatcgcct
     4561 tgcggagtaa agccgggcaa agaaagatag gaaggagggt gtgccggacg cggggtagag
     4621 ggcgtggcgt ctgctgggtg ttggacggca agactagatc tgtattcttg cgccatgcac
     4681 gattcaaaat tcaccccaag gcatgcctcg ctgtccgaat tgaggttcct gatccgccca
     4741 acgctgggct tcagcagcag tgctattggg actccttagc ctttgccgca gtcggctgaa
     4801 gcggttagtt atgtggcgtc gcaatttccc actcaatgat ttttgaatct atcccgacat
     4861 cacccgctgc tttgaagaac atctcctgct ctcttgtggg cttatcccaa tgagggcatt
     4921 tcacctcgac aaatcttact cggcatgcct gctcatccca gattactaaa tctggacaac
     4981 ctcgctttaa gttcgcagtt tcaaatatct ggaagagcac ctcagaaaca aaacctggca
     5041 acgagacccg ttttgtcgac aaagcggact ttgaagtgcc tgatgaactt tgaaggtcaa
     5101 cgactccgct cgctatccct cgtgaattca tccaagcccc cctctcgcct ggcctcaact
     5161 ccaaagacaa aatagcgagc tctgctggca aaacgcgccc cttgaagatg atcagcggct
     5221 tgttccacca ccgttgttta gccgactcaa atcttaggat cgtgctcacg cttttctctc
     5281 ttaggccaca taacaatcaa gctgagcggt gtccgagaag cgttcgtcta ccgagcagcg
     5341 cagatcggcg tctactccaa cgcccggtta ggctgtacgg cttcatttgt ccgccgattc
     5401 tatgcgacga tcagtgcggg agcgctcatg cgcaagatac cgatccgcga ctttaaggcc
     5461 gagcctcgat ttgtgctcga tctcggtacg cacctacacg ccgagtttca aatggcctgg
     5521 aattcatcgg cagagtggtc ccatcggtag ttgttgcaga gtgcatgcgc cggcaggtag
     5581 ttgttggcag tgtgagcacc accgacgcta tgtgagaaga catgatctgc ctgccacttt
     5641 ccgtcaatgg agccgccgca gatgtggcaa cgtcccgaag tctttttgaa cacttctttg
     5701 cgctcgacag gtgagagact gacgcggcga aaccgttcta tgaagctctg ccttccaatg
     5761 ccggcgtgtc agccgcaact tacggaggaa ttcagcaaat gcctcaggcg tcgccgcgat
     5821 gactcttgtg gctatcttct ccggggttga gacttcgcta ttaagcgaac tgctgagagg
     5881 cctccgatta gctcctggta tatcggttcc atccgacgct tccccgctca aaatatccca
     5941 atagttgtct cttgtcaggc tttttcaagg ttccacttgt caggtgagac agcgaagcct
     6001 ggcggtatgc caaactattg gatcgcagga aattaggcgg atttatgttt ggcgttgcga
     6061 gagccgcttc gattctcgac gtaaccgaaa aatacaaatg gaatggtcca agctctaaca
     6121 agcagtgtat cgccttattg ggcgacggct catgtgccgt gacgatc
//
LOCUS       DATNDD010000021         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_269556, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000021 DATNDD010000000
VERSION     DATNDD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269556"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VN647_00540"
     CDS             <1..792
                     /locus_tag="VN647_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWG95538.1"
                     /translation="TEGKPIPKGFNLVTFPDPVYCGRISTGTGWRILDEFSVSADRGL
                     KDTVVLLIDAEKGKPFKFVPPTIEARDCRFLPFVSVVKNRAEVRVVNMDPVFHDIQAY
                     ETSDLGPRVLFNTPLPMNPLHVRDAGSESHEHLAGQPMVEVVKMTKNRRFFVMQCGFH
                     AYMESWGFAVDNPYYMVTDADGRFSLSDVPAGEYTLMAWHPGVGTVLEKKISVSAKQT
                     HQVDFTFESPKGRRSVHEIAENPHYGLEVLGKSVEIRPTLQLQTP"
     gene            826..1758
                     /locus_tag="VN647_00545"
     CDS             826..1758
                     /locus_tag="VN647_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWG95539.1"
                     /translation="MTRVARCLVNLSAVLVSALVLSSPTPATAYEVIDIQHGGTIEGT
                     VTLDGPVPEPKGFNLITFPDPAYCGRISNGRGWRLLHDFVVGPQGGLRNAIVLLEGVE
                     TGKPFEVSVPLIEARDCMFQPFMTIVRNGHAVEVINMDPVMHDIQGYETSPEAGARVL
                     FNNPLVMNYQHRRGDIHATHNHAPGASLVGPIYLNKGRRTFYMQCGFHAYMESWAMAV
                     NNPYYSLTDAEGRFSITGIPPGTYQLVVWHPQTGPGMTRTVVVQPDGKLREQLSLPAP
                     KGNRTAYKVMDNPRFGPESLGYSIEIQPLVERQQ"
     gene            1984..>2641
                     /locus_tag="VN647_00550"
     CDS             1984..>2641
                     /locus_tag="VN647_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWG95540.1"
                     /translation="MRLRPIIVFVLAWCIPMAESAFGGMASYIQQSQTLYDKKEYQKA
                     LAELDKLDSQTSAAPDARRLRIRTLLKLGNPKNALTNYDELVQALKHEDQSILRELSL
                     GFVLVLTKDMREQMRGVAYTALKEWHSSESIPYLEDGLSDGSGLVRALAAEGLAKLDE
                     GRRSARFRKALEDQAALVKEAVLKGLAKSDDPSVIPLVEPALKDPEVRVRVAAAAVLC
                     N"
BASE COUNT          603 a          679 c          746 g          613 t
ORIGIN      
        1 acggaaggaa agccgatccc caaagggttc aacctcgtca cctttcctga tcctgtctat
       61 tgcggaagaa tttccaccgg gaccgggtgg aggattttgg acgagttcag cgtttcggca
      121 gatcgaggcc tcaaagacac ggtcgtgttg ctgatcgatg cggagaaggg aaagccgttt
      181 aagttcgtgc cgcccaccat cgaggcccgc gactgccggt tcttgccttt tgtcagtgtg
      241 gtcaaaaacc gagccgaggt gcgggtcgtc aacatggatc cggtgtttca tgatattcag
      301 gcatatgaaa cgtcggacct aggtccacgc gtattattca atacaccgct tccgatgaat
      361 cccttgcacg tacgtgatgc cgggtccgag agccatgaac atctggccgg acaaccgatg
      421 gtcgaagtcg tcaagatgac gaagaaccgt cggtttttcg tcatgcaatg tggatttcat
      481 gcctacatgg aaagctgggg gttcgccgtg gacaatccct attacatggt gaccgatgcg
      541 gatggacggt tttcgctgtc cgatgtgcct gccggagagt atacgttgat ggcgtggcat
      601 cctggagtag ggacggtgct cgaaaagaaa atatcggtgt cagcgaagca gacgcatcaa
      661 gtagatttca cgttcgaatc tcccaaaggg cgccgaagcg tccacgaaat cgcggagaac
      721 ccgcattacg ggttggaggt gttgggaaaa tccgtggaga ttcgtccgac cctccaacta
      781 cagacaccat aaataccgtt cgtttgaatt gggagagcat tgccgatgac gagagtcgcc
      841 cgttgtcttg taaatctcag tgccgtcctt gtttcggcgt tggtcctttc gtcacccaca
      901 ccggcaaccg cctacgaagt catagatata caacatggtg ggactatcga agggacggta
      961 actcttgacg gaccggtgcc ggagccgaag gggttcaatc tgattacctt tccggatcct
     1021 gcctattgcg gccgtatctc caatggtcgt gggtggcgct tgctgcatga ctttgtcgtc
     1081 ggtccccaag gaggattgcg aaatgccatt gtgctcctgg agggggtcga aaccgggaaa
     1141 ccatttgagg tttctgttcc cttgatcgaa gcccgcgatt gcatgtttca accgttcatg
     1201 accattgtcc gtaacggaca tgcagtcgaa gtcatcaaca tggatcccgt tatgcacgac
     1261 atccaaggct atgagacatc gccggaagcg ggtgcgcgag tattattcaa taaccctttg
     1321 gtcatgaatt atcagcatcg gcgaggggat atccacgcca cccacaacca tgcgccgggg
     1381 gcctcgctgg tcgggccgat ttatttaaac aaggggcgac gtacgttcta tatgcaatgt
     1441 ggcttccatg cctatatgga aagctgggcc atggccgtga acaatcctta ttattccctg
     1501 acggatgccg aggggaggtt ctcaatcacc ggcattcccc ccgggaccta tcagttggtg
     1561 gtatggcacc cgcagacagg acctggaatg acaaggacgg ttgtggtgca accggacgga
     1621 aagcttcgtg agcaactttc actgcccgct cccaagggga atcgaactgc gtataaagtc
     1681 atggacaatc ctcggttcgg cccggagtct ttgggatact ctattgagat tcaaccgctc
     1741 gtcgaacgtc aacagtgaca ctcatgtgtg tgtgctccga agaagacttg ccgatcggtt
     1801 tctcttgtcc tcctcgttgt tgttttaata tgtgccagtg ccgattctga aaggcggcaa
     1861 tattctcccc tttgagccgg aattctccag catgctcgtc aaacgcgtac acggcaagaa
     1921 ataatcacca aggcatgaat aaccagccgg caccgtgaag ggtagagaat ggacccgctg
     1981 aacatgcgac tacgtccaat catcgtgttt gtcctggcct ggtgtattcc catggctgag
     2041 tccgcgtttg gcggaatggc atcatacatt cagcagagtc agacactcta tgacaagaag
     2101 gagtatcaga aggcacttgc agagttggat aaactcgatt ctcagacgtc ggccgcaccg
     2161 gacgctcgtc gactcagaat tcgtacactg ctgaaactgg gcaaccccaa gaatgccctt
     2221 accaactatg atgagctcgt acaggcattg aagcatgagg atcaatcaat ccttcgtgag
     2281 ctctctcttg gatttgtcct cgtcttgacg aaggacatgc gcgagcaaat gcgtggtgtg
     2341 gcctataccg ccctcaagga atggcacagc tccgagtcga ttccatattt ggaagatggg
     2401 ctcagcgacg gatccggtct cgtccgagcc ttggcggctg aagggcttgc gaaacttgac
     2461 gagggccgcc gctcggctcg attccggaag gctctggaag atcaggcggc gttggtgaag
     2521 gaggcggtac tcaagggact tgcaaaatct gatgatcctt cggtcatccc gctcgtcgag
     2581 ccggcgctga aagatcctga ggtacgggta cgagttgcag ccgcggcggt gctctgtaat
     2641 c
//
LOCUS       DATNDD010000022         3300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2865449, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000022 DATNDD010000000
VERSION     DATNDD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865449"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VN647_00555"
     CDS             <1..354
                     /locus_tag="VN647_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWG95541.1"
                     /translation="KPELTGQNDFAIKQPTSGAIPYTIHGPAPGDQGMSDRAYVLINV
                     LPGQTAAVVKALGEIKEIKTIDPCWGKPDIIAIVDIPDQDALTQLVLSRIHRIEGVTQ
                     TDTHLVYRLKEARTK"
     gene            351..851
                     /locus_tag="VN647_00560"
     CDS             351..851
                     /locus_tag="VN647_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95542.1"
                     /translation="MIRKDCLAIVALTVFTFGCTSVPSQHNSQADVVDVTLPVSADRV
                     KAAVTKVLADDDYDVDWKDGSQLQTGYRGEQPSIWDWLVKGRFGVVKSRAEASVTPET
                     DQSSRLHLQISSEGKQTMFQGWGPTAPQVPQSAENKLRLIKNELKIVQNTYTTETFYG
                     SYGLKP"
     gene            complement(848..2257)
                     /locus_tag="VN647_00565"
     CDS             complement(848..2257)
                     /locus_tag="VN647_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWG95543.1"
                     /translation="MQPTTILVADDDAVARELLAEALRKEGYHVEAFAGGEEVIARGR
                     QGRVDLVLTDIRMGAVDGLTVLREFKRVSPNTAVVVLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKKEDIKLVVKRGLDHCRLLQENARFREKLKNKGEWSPLVGSSTAMLEVYKLVAR
                     VAESKSTVLLQGESGTGKELIARAIHTNGSRRDKPFIPVNCGALPDTLLESEMFGYEK
                     GAFTGAAGPKVGLFESANGGTLFLDEIGELGQALQVKLLRVMQDQEVRRVGGTTSTRV
                     DVRIIAATNRDLEQLVKEGKFRDDLFYRLKVVPITLPSLLDRREDIPMLVHHFLQKCA
                     GGTDHAVRGVLPETMALLIQYRWPGNVRELENAIERAVSLSHGPLLTPEDLPEVIRQG
                     ASEADARLSQVDQLDEVCLTLEEVEKRHLTRVLKETKGNKVKAAKILGIDRRTLYRMA
                     ERFGLDLGEETEGAEKEPA"
     gene            complement(2257..>3300)
                     /locus_tag="VN647_00570"
     CDS             complement(2257..>3300)
                     /locus_tag="VN647_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG95544.1"
                     /translation="LLVTTLRNRTIMLVTVLACVVATYSGLLQPTKPGRFQTAVINRS
                     VVVGVLLLVAYLGVSWEERKAREEATRAALAQETENLLKANTQLVQAKDQLNRSERLA
                     AVGQLVASVAHEVGTPLHSIAWHVQALAEEPGATPEMRKRVAIIDDQITRVVRIIQDL
                     LSSTRPRQPEPQWLPVEEVVGPSAILMESAFHEKGIVLAVDIPKDLPLIWADKEKIHQ
                     VLVNVLTNALAATPSQGKVTIMAGSRKASSDELDRGRLVAHGMSPVVITIEVQDTGCG
                     MPEEDARKAFEPFFTTKAVGKGTGLGLFLSRESVVAHGGSLALMSESGCGTAVTMTLP
                     AMRREAIQTREEA"
BASE COUNT          739 a         1023 c          799 g          739 t
ORIGIN      
        1 aagccggagt tgacagggca aaatgacttt gctataaagc agcccacttc cggggcaatt
       61 ccctatacga ttcatggtcc cgctccggga gatcaaggca tgtcggaccg ggcatatgtg
      121 ctgatcaatg ttttacctgg acagacagct gcggtagtca aagcgctggg tgaaattaaa
      181 gagataaaaa cgatcgatcc ctgttggggc aagccggata tcatcgccat agtcgacata
      241 ccggaccagg atgccttgac tcaactggtc ttgagtcgta tacatcgtat agaaggagtg
      301 acgcagaccg atacccacct cgtataccgg ctgaaggagg ccagaacaaa atgatccgaa
      361 aggactgtct cgccatcgtc gccctcaccg tcttcacctt tggatgtacc agcgttccgt
      421 cacaacacaa ctcacaagca gatgtcgtcg atgtcacatt gcctgtgtcg gcagaccggg
      481 tgaaagcggc agtgacgaaa gtcctggctg atgatgacta cgatgtggac tggaaagacg
      541 gatctcagtt acagacaggt tacaggggtg aacagcccag tatctgggat tggctcgtga
      601 aaggacggtt cggtgtggtc aagagccgtg ctgaagcgtc cgtaacgcca gaaactgacc
      661 agagctcccg tctccatctg caaatatcgt cggaaggtaa gcaaacgatg ttccaagggt
      721 ggggccccac cgcacctcaa gttcctcaaa gtgcggaaaa caaactgcgc ttaatcaaga
      781 acgaactgaa aatcgtacag aacacgtaca caacagagac cttttacgga tcttacggat
      841 tgaagcctta agcaggctct ttttccgctc cctcagtctc ttctccaaga tcaaggccga
      901 agcgctctgc catacggtag agcgttcgtc tgtcgatgcc aagaattttt gcggccttta
      961 ccttatttcc tttcgtttcc ttgagcacgc gagtcaaatg acgtttttcc acttcttcca
     1021 aagtcagaca cacctcatcg agctgatcga cttgcgaaag tcgtgcatcg gcttctgacg
     1081 caccctggcg aatcacttcc ggcaaatctt ccggcgtcag taatggacca tggctcaatg
     1141 acacggcgcg ctcaattgca ttttcaagct ctcgaacgtt gcctggccac cgatattgga
     1201 tcaacaatgc catagtttcc ggcaagactc cccgtaccgc atggtccgtg cctcccgcgc
     1261 acttttgcaa gaagtgatgc accagcatcg gaatatcttc tcgccgatcg agcaacgacg
     1321 gtaacgtgat tggaacgact ttcaaccggt agaagaggtc gtcccgaaac tttccttcct
     1381 tcaccagttg ctcaagatct cggttggtcg cggcaatgat ccggacatcg actctggtgg
     1441 atgtcgtgcc cccgacccgg cggacttcct gatcttgcat gacccgcaac aacttcactt
     1501 gtaacgcctg tccaagctca ccgatctcgt cgagaaacag cgtccctcca ttggctgatt
     1561 caaagagccc gaccttaggc cctgctgcgc cggtgaaagc gcctttctca tatccgaaca
     1621 tttctgattc cagcaaggta tcgggcaacg caccgcaatt gaccgggata aacggcttat
     1681 cccggcgcga gccattggta tgaatggccc gcgcaatgag ctctttgccg gtaccgcttt
     1741 ctccctggag caggaccgtg cttttgcttt cagccacacg cgcgaccaac ttgtacacct
     1801 ccaacatcgc cgtgctgctt ccgaccaagg gagaccactc gcctttattc ttcaactttt
     1861 ctcgaaatcg cgcattctct tggagcaatc gacagtgatc cagcccccgt ttgaccacca
     1921 gcttgatgtc ttctttcttg aacggcttcg ccagataatc ataggcaccc tgcttaatcg
     1981 cttcgatcgc gccttccaga gagccgaacg cggtgagcac caccacggcc gtattcggac
     2041 tcactcgttt gaactcccgc aagaccgtca gcccatccac cgcgcccatg cggatgtccg
     2101 tcaacaccaa atccacccgc ccctggcgtc cacgtgcgat cacctcttct ccaccggcaa
     2161 aggcctcgac atgatatcct tccttcctga gagcctctgc caacagctca cgggcaaccg
     2221 catcatcgtc ggccaccaga atcgtcgttg gttgcattac gcctcctctc tcgtctgaat
     2281 ggcttcacgt cgcatcgccg gtaatgtcat cgtcacggcc gtgccacacc cgctctcact
     2341 catcagcgcc aggctgccac catgagctac caccgattct cggctcaaga atagccccaa
     2401 gccggtcccc ttgccaaccg ccttcgtcgt aaaaaacggt tcaaaggctt tccgtgcgtc
     2461 ttcctccggc ataccgcatc ctgtgtcctg cacctcgatc gtgattacca ccggcgacat
     2521 gccgtgtgcg accagccgac cgcggtccag ttcgtccgac gacgccttgc gacttcctgc
     2581 catgatggtg actttcccct gtgagggcgt tgcagcaagc gcattggtca ggacgttcac
     2641 caatacttga tggatttttt ccttgtccgc ccaaatcagc ggaaggtcct ttggaatgtc
     2701 aaccgccaga acgatccctt tctcatggaa cgccgactcc atcaggattg cagaggggcc
     2761 gaccacttcc tccactggaa gccattgcgg ctcaggttgg cgaggtcgtg tagaagacaa
     2821 taaatcctga ataattcgaa ctacacgcgt aatctgatca tcgatgatag ccacccgctt
     2881 cctcatctcc ggcgtggcac caggctcctc ggccagagcc tggacatgcc acgcgatcga
     2941 gtgaagcggc gtacccacct cgtgcgccac ggaggccacg agctgtccca ctgcagccaa
     3001 ccgttccgac cggttcagct gatctttcgc ttggaccagt tgtgtgttgg ctttaagtag
     3061 attctcggtt tcttgagcca gcgccgccct cgtcgcctcc tctcgcgcct tccgctcttc
     3121 ccagctcaca cccaggtatg ccaccaacag caagacgccg acgacgacac ttcggttgat
     3181 gacggcggtt tgaaatcggc ccggcttcgt cggttggagt agacctgaat aggtcgcaac
     3241 gacacaggcc agcaccgtca ccaacatgat ggttctgttc cgaagagtgg tcaccaacaa
//
LOCUS       DATNDD010000023         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3996018, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000023 DATNDD010000000
VERSION     DATNDD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996018"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VN647_00575"
     CDS             <1..407
                     /locus_tag="VN647_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95545.1"
                     /translation="TLILTAPLGSGIHQEPYHYYGGYTPWWYERFLKAAGFEEINIEP
                     NEGSFRFFGQESIRFIQTTSPWSTSLPVGIRLLWFPMWIVLLPILGLIVPIFCKWLDR
                     FDRERRFTVGYHVTARRARGAARKGGRLETVC"
     gene            533..1735
                     /locus_tag="VN647_00580"
     CDS             533..1735
                     /locus_tag="VN647_00580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG95546.1"
                     /translation="MKILCVLGEHAYGDPARGEGPEYANFIPALRRLGHEVTFFESLS
                     RKPYDDFSHLNHALLKRVEETSPDVVFCVLMHYEVWIETIQVIRNSGAWVVNWATDDS
                     WKYPMFSKLIGPKFDLFVTTYPQMGERYHHDGINSVCFSQWAANGDTLISSLPASACR
                     YPVSFVGAAYGNRRAMIARLRHEGIEVVCFGHGWPEGPIETKRMGEIFRESQISLNFS
                     DGAYSRSDFTSRQIKARIFEVPGYGGCLLTEKAPNLDRYFHVGEEILAFEGADEMIQQ
                     VKSLLSRPERRDEVARCGFERVRRDHTYERRFEELLRELPQRIPRRPRIPIKWDGFEA
                     AVRGYRTSPVLRMIRFLLVFVASLVWGKKRGPRAARRLVFELSWRLRGVWTYSAAGWP
                     GRMFYKES"
     gene            1736..>2267
                     /locus_tag="VN647_00585"
     CDS             1736..>2267
                     /locus_tag="VN647_00585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWG95547.1"
                     /translation="MDTGPLVTVAMSAYNAAATIDLAIRSIRSQSLEDWELIVVDDGS
                     TDSTREIVSFHAQDPRIRFIQEPSGNRGLAVRLNQCVRLAKGQYVARMDADDMAYPQR
                     LERQVQFLEEHRDIDLLGTGAVIFKGEGEIIGCYPTANSHEAICRRPWWGFPLAHPTW
                     MGKRTWFLSHPYSDEDT"
BASE COUNT          477 a          579 c          673 g          538 t
ORIGIN      
        1 gaacgctcat tctcaccgcg ccgcttggct cagggattca tcaagagccc taccattact
       61 acggcggcta tacaccatgg tggtacgagc gctttctgaa ggcggccggg tttgaggaaa
      121 tcaacattga accgaacgaa gggtcgtttc gattttttgg gcaggaatcc atacggttca
      181 tacaaacgac cagcccgtgg agtacgagtc taccggtagg gatcaggctg ctgtggtttc
      241 cgatgtggat cgtgctcttg cctatcctcg gattgatcgt ccctatattc tgcaaatggc
      301 tggacaggtt cgacagggaa cggcgcttta cagtcgggta ccacgtgacg gcgcgacggg
      361 cgcgcggagc cgcgaggaag ggaggcagac ttgaaacagt ctgttgaccg accgagcggc
      421 gagcccgccc gccaacttgc cgtcgaagtc gcatgttggc ttgtcgcagc ggcgaatcca
      481 caattgcagc agaagtgttc atgaataatg tgggctaagt cgtcgtgatc gcatgaaaat
      541 cttgtgtgta ttgggagagc atgcctacgg cgaccctgcg cgcggagaag gacccgagta
      601 tgcgaatttt attcctgcct tgcgccgttt ggggcatgag gtgactttct tcgagtcgtt
      661 gtcccgcaaa ccgtacgatg atttttctca cctgaatcat gcgttgttga aacgtgtgga
      721 ggaaacgagc ccggatgtcg tcttctgcgt cctgatgcac tatgaagtgt ggatcgagac
      781 catccaggtg atccgcaata gcggcgcctg ggtcgtcaat tgggcgactg atgattcctg
      841 gaagtatccg atgttttcca agctgatcgg tccgaaattc gatctcttcg tgacgaccta
      901 cccgcagatg ggtgaacggt accatcatga cggcatcaac tcggtctgct tctctcagtg
      961 ggcggccaac ggcgacacct tgatttcctc ccttcccgcg tcagcctgcc gatatcccgt
     1021 gagctttgtc ggtgcagcgt atgggaatcg tcgagccatg atcgccaggc ttcgtcatga
     1081 aggcatcgag gtggtttgtt tcggtcatgg atggccggaa ggtccgatcg agaccaagcg
     1141 tatgggggaa atctttcgcg aatctcagat cagccttaat ttcagtgatg gagcttacag
     1201 tcgttctgat tttacaagtc gacagatcaa agcacgtatc ttcgaagtgc cgggatatgg
     1261 gggctgtctc ctgaccgaga aggccccgaa cctcgatcgg tatttccatg ttggggaaga
     1321 aattctggca ttcgaaggtg ccgacgagat gatccaacag gtcaaatcgc tgctctctcg
     1381 tcctgaacga cgggatgagg ttgcgcgctg cggttttgaa cgagtgcgac gtgaccatac
     1441 ctatgagcga cgcttcgagg agttgctgcg agagctgccc caacgtattc cccgacggcc
     1501 tcgaattcca attaaatggg atgggttcga agccgctgta cggggttacc gcacgagtcc
     1561 ggttcttcgt atgatccgat tcctgttggt gttcgtcgca tccttggttt ggggaaaaaa
     1621 acgcggcccg cgagccgcgc gtcgtctggt ttttgagttg tcttggcgtc tgcgaggggt
     1681 ctggacctat agtgccgccg gctggccggg gcggatgttt tacaaggaaa gctgattgga
     1741 cacaggaccg ttggtcacag tggcgatgtc ggcctacaat gccgccgcca ccatagatct
     1801 tgccatcaga tccatccgat cccaatcttt agaggattgg gagctgatcg tcgtcgatga
     1861 cggctccacc gattcgacac gagagatcgt gtcgttccat gcgcaggatc ctcgaatccg
     1921 attcatccaa gaaccatcgg ggaatagagg cttagctgta cggttgaatc aatgtgtgcg
     1981 tctcgcgaag ggacagtatg tcgccagaat ggatgccgat gacatggcgt atcctcagcg
     2041 tctcgaacgg caggttcagt tcctcgaaga gcatcgtgac atcgatctgc tgggaaccgg
     2101 cgctgtgatc ttcaagggag aaggcgagat catcggctgt tatccaacgg caaactcaca
     2161 cgaggccatt tgccggagac catggtgggg gtttccgctt gcccatccga catggatggg
     2221 caagcggacc tggttcttat cgcatcccta ttcagacgaa gatactc
//
LOCUS       DATNDD010000024         6128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1144645, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000024 DATNDD010000000
VERSION     DATNDD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144645"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2291
                     /locus_tag="VN647_00590"
     CDS             <1..2291
                     /locus_tag="VN647_00590"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HWG95548.1"
                     /translation="SFGFLVSESGSGFTWSLNSHENQLTPWSNDPVSDPSGEVLYIRD
                     DDTGEVWGPTALPIRDDPAPYVACHGQGYSRFHHGSHGVLAELLQFVPPEDPIKISRL
                     TLQNMSGRSRRLSVTAYVEWVLGSSRSESAPYIITEIDPGSRALFARSMLGGEFAGRI
                     AFTDLAGTHTSWTGDRTEFLGRNGTFDRPLALQAGGELSRKVGAGLDPCGALQLSIEL
                     DAGERAEIVWFLGQTAGSEQARLLLNHYRGMDVNALLREVTRRWDDRLGVVQVHTPER
                     AMDIFLNRWVLYQTLVCRVWARAAFYQLSGAYGFRDQLQDVMALSVSARDITREHLLR
                     AAGRQFVEGDVQHWWHPPSGRGVRTRISDDLLWLPYAVIQFLEVTGDMTVLDEMVSFL
                     EGPALAEGQHESYFEPQMSRTRATLFEHCARALDRSLVVGSDGLPLMGTGDWNDAMNR
                     VGRQGKGESVWLGWFLHSVLWEFAKVAAQRGERVRAETWRLHVSALKAAIERDGWDGE
                     WYRRAYFDDGTPLGSAADPECRIDSIAQSWGVISGAADPDRGARAMAAVERYLVNRRD
                     GLIRLLAPPFDQMPRDPGYIKGYVPGVRENGGQYTHAAVWTVLAFAALGEGDKAGELF
                     RMLNPVHRVSSRASVQRYKVEPYVVAGDVYAEPPHVGRGGWTWYSGAAGWLYRAGVEW
                     MLGFRLRGTILSIDPCIPRQWPGYSIRFRYHSAVYDIAVENPRFVSRGVTLAELDGKP
                     LISRDNIPLVQDGDHHIRIVLG"
     gene            2627..3082
                     /locus_tag="VN647_00595"
     CDS             2627..3082
                     /locus_tag="VN647_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG95549.1"
                     /translation="MLEQKNKMTKWELPGVPAGGLRKVGQIRGTNTLILHAGQNGMLV
                     ALELLSTHTPGAPVVDDRNQFIGFISEFDVLRSLEAGKDLSKLTAEDIMVHDQISVTA
                     ETTIEEAVKTMEEKRLHNLPVKKDGKIAYSVTRHDLLRAWIGLGTSGED"
     gene            3943..4440
                     /locus_tag="VN647_00600"
     CDS             3943..4440
                     /locus_tag="VN647_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HWG95550.1"
                     /translation="MSTPHHPRDKKDRSVGARKRDRYKAKGKLQEPSVCRECQAVYHK
                     GRWVWGPVPSDSHEVMCPACERIRDRAPSGVLLLTGEFVAKHRDEVLGLARNEEVRLK
                     AEHPLARIIKIEDQTEAPEGVVITTTDPHLARRIGEALHHAHRGTFACRYEENEDLLR
                     ANWEC"
     gene            4554..4730
                     /locus_tag="VN647_00605"
     CDS             4554..4730
                     /locus_tag="VN647_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95551.1"
                     /translation="MQLTLAPQEQVVLSWAVESAISDLGTEIGHTDNQAMRQDLQERK
                     GILLTILGRLRVTT"
     gene            4988..5389
                     /locus_tag="VN647_00610"
     CDS             4988..5389
                     /locus_tag="VN647_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95552.1"
                     /translation="MFVYLLVFAIVIGFAFSGFAQFVDKGEYVEVAQGTKLCPDVILE
                     KSKGRLDPENVYWRVTSKVRTNGTQTVAAFVLETLYDRKSGETTIKERRYDEDYAMLF
                     KRSPEHKGMLLMISPKDGRVEATVEICRKKK"
     gene            5928..>6128
                     /locus_tag="VN647_00615"
     CDS             5928..>6128
                     /locus_tag="VN647_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95553.1"
                     /translation="MRFIMIAIVGYVFLGMTVLALANPAMLPKHPGYPSSGEFANDTG
                     QKNLTYRQSIEEAAKSGDTTMGT"
BASE COUNT         1306 a         1542 c         1880 g         1400 t
ORIGIN      
        1 cgtcgttcgg attcctcgtg tcggagtccg gatccgggtt tacttggtca ttgaacagcc
       61 atgagaatca gctgacaccc tggtcgaacg atccggtgtc tgatccgtcg ggcgaggtgc
      121 tctatatccg tgatgacgac accggagagg tctggggtcc gacggcgctg ccgattcgcg
      181 atgatccggc accgtacgtc gcctgtcacg gacagggcta tagccgcttc catcatgggt
      241 cgcacggtgt tttggccgaa ttgctgcaat ttgtaccgcc tgaagatccg atcaagatct
      301 ctcgattgac actgcagaat atgtccggcc gatcgagacg cctttcagtc acggcctatg
      361 ttgagtgggt gctcgggagt tcccgcagtg agtccgcacc ttacatcatc acggagatcg
      421 atccggggag cagggcgctt tttgcgcgca gcatgctagg cggcgagttc gcaggacgca
      481 tcgcgtttac cgatcttgcc ggaacccata cgtcatggac cggtgatcgt acggaatttc
      541 ttggtcgtaa cgggactttc gatcgtccct tggcgctgca agcaggaggg gaattgtctc
      601 gaaaggtcgg cgcgggtctc gatccttgtg gggcccttca attgtcgatt gagttggacg
      661 caggcgaacg tgccgaaatc gtctggtttc ttggtcagac agcggggagt gagcaggctc
      721 gtctcctgct caatcactat cgtgggatgg atgtgaacgc gctcttgcgt gaggttaccc
      781 gccgatggga tgaccgattg ggtgtcgtac aggtacatac gccggagcgc gctatggata
      841 tcttcttgaa tcgctgggtg ctctatcaga cgctggtttg ccgggtctgg gcacgtgcgg
      901 ccttttacca gttgagcggg gcgtatgggt ttcgtgatca acttcaagat gtcatggccc
      961 tgagcgtatc ggcacgcgat atcacacgtg agcatctgct gcgggctgct gggcgtcagt
     1021 tcgtcgaggg ggacgtccaa cattggtggc atcctccgtc cggacgcggt gtgcggactc
     1081 gcatttccga tgatctactc tggttgcctt acgcggtgat ccaatttctt gaagtgaccg
     1141 gcgacatgac tgtgctggac gaaatggtgt cctttctgga aggcccggca ttggctgagg
     1201 ggcaacatga atcctatttt gaaccacaga tgtcccggac acgcgctacc ctctttgaac
     1261 attgtgcgcg tgcgttggac cggagccttg tcgtcggcag tgatggcctt ccgctcatgg
     1321 gtaccggaga ctggaatgac gcgatgaacc gagtcggccg gcagggcaaa ggggaaagcg
     1381 tctggctcgg ctggtttctg cattcggtgc tctgggagtt cgccaaggtg gcggctcagc
     1441 gtggtgagcg tgttcgtgct gaaacgtggc ggctccatgt gagtgcgttg aaggctgcga
     1501 tcgagcggga cgggtgggac ggggaatggt accgccgcgc gtacttcgat gatgggacgc
     1561 cactcggttc cgctgcagat ccggaatgcc gcatcgattc tattgcccag tcctggggag
     1621 tgatcagcgg cgcggcagat ccggaccgtg gcgcacgcgc catggccgcg gtggaacgat
     1681 atcttgtgaa tcggcgggac gggctaatcc gtctgctggc accacccttt gatcaaatgc
     1741 cgcgggatcc tggctatatc aaaggctatg tccctggcgt gcgagaaaac ggcggtcaat
     1801 acacccacgc ggcggtctgg accgtgctgg ccttcgcggc gttgggtgag ggtgacaagg
     1861 ccggcgagtt attccgcatg ttgaatcctg ttcatcgggt ctcctcgcga gcgagcgtcc
     1921 agcgctacaa agtcgagccc tacgtcgtag ccggcgacgt gtatgccgag ccgccgcatg
     1981 ttggacgcgg gggatggact tggtacagcg gcgcggcggg ttggctctat cgggccggtg
     2041 tggaatggat gctcggcttc cgcttgcgtg gtaccatact ctccattgac ccttgtattc
     2101 cgcgccaatg gccggggtat tcgattcgtt tccggtatca ttcggcggtc tatgacatcg
     2161 cggtggaaaa tccacgcttt gtgagccgcg gcgtgacgtt ggcggagctt gatggcaagc
     2221 cattgatcag ccgcgacaat atccccctcg ttcaggacgg tgatcaccac attcgcattg
     2281 tcctcggatg atgcagcggg ctgcggctag ggtcactcct agcacggggc cgtggtatct
     2341 ccaagctaga atcaattaag agaactgaag gaggtgtcgt tatgggagtc aacctattac
     2401 acattgcttt tgatcggggt gaaagagatc gtgattcgcc tttgtagcaa tattcctccg
     2461 tcagtgttct cccgttgggg tgtttcacct caagcccatt ggtttaaatc ctcttggtct
     2521 ccccatcacc cgaattctcg atgggtcgaa ttggtatggc cttcgcttct gtgacgagta
     2581 gttattcaat catcatgagg tatggtgaac ctgaaaggag tgtacgatgc ttgagcaaaa
     2641 gaacaagatg acaaaatggg aattacccgg ggttccagcc ggaggattgc gaaaggtggg
     2701 gcagatcaga gggaccaata ctctcatttt acacgccggc caaaatggaa tgctcgtggc
     2761 tctggaatta ctgtccactc atactccagg cgcgcctgtg gtcgatgatc gcaaccagtt
     2821 catcggattc atcagcgaat ttgatgttct ccgttcattg gaagccggca aggatctttc
     2881 gaagctgacc gcagaagaca tcatggtcca cgatcaaatt tccgtcacgg ccgagaccac
     2941 gatcgaagaa gcggtgaaga cgatggagga gaagcgcctg cataatcttc ctgtgaagaa
     3001 ggatgggaag attgcttact ctgtgacgcg gcatgatctt ctgcgagcct ggattggcct
     3061 cggcacttcg ggcgaagatt gagaagagtc gccttcgcga tgggcggagg ggcaatcgaa
     3121 agcggagtgc gctgggctta gagtggggca tggcagaaac ttggtcatat gggggacggg
     3181 gatgtgaagg atcgttcgta ggcacagggg aactggtggt tcttcgcaaa ctgagcaatt
     3241 ctgatcagac cgcgaatcgt gcgtagggta gactaacttc acatccaaac gcaaacgtcg
     3301 tttggtgcct gcgtgtcaga acacacgggg tgctgccgat cagtttgacc atcttcgcgg
     3361 ctggtgttcg accgtgcacg cgaatgatcg cgggggcgac cgctcaatat gcgaaagaag
     3421 gagcctcctg tgaccacatt cttaagaata agaacaggta ctgcgcagtg cttgctggcc
     3481 gcggtctctc atcgtccgga aggccggatg aaaggggacg ggtaaataga acccacggca
     3541 tgatcgaatt gagcatgtcg gagccattca tttcctacca tgcgaccggc tgtttcggac
     3601 tgtcacggtc gtcagtcaga tattcaagat tggtcctgcc gccatgtatc tagattgtgg
     3661 cactagtgga caggaggtcc ggctttccct ggcccagaac accacagtat cccatgaggc
     3721 gatcgcacag cagagcacgg actctcctcg tcgtctcatg atttccacgg atttgagcat
     3781 gtcgcacatc cgttgcagaa tggcattcca catgcagatt cacgattggt atgacaatga
     3841 ggacttacga aacctgcctg tctgaatcag gaacgatacg ctggggggat ctctgtctag
     3901 ccggattcgg cggatatctc tcatgcgatg gagtgatgga acatgtcgac accacatcat
     3961 cctcgagaca agaaagatcg atcggtcgga gctcgaaagc gcgaccggta caaagccaag
     4021 ggcaaacttc aggagccgtc ggtgtgtcgt gagtgccagg cggtgtatca caaggggcgg
     4081 tgggtgtggg gccctgtccc atcggacagc cacgaggtga tgtgtcctgc ctgtgaacgg
     4141 atccgggacc gtgcgccgag cggggtgctg ctgctgaccg gtgagtttgt cgccaaacat
     4201 cgggacgagg tgcttgggct cgcccgcaat gaagaagttc gtctcaaagc cgaacatcct
     4261 ctagctcgaa tcatcaagat tgaggaccaa accgaagcac ctgaaggggt ggtgatcacc
     4321 acgaccgatc cccatcttgc gcggcgcatc ggcgaggcgt tgcatcacgc ccatcgtggc
     4381 acattcgcct gtcgttatga ggagaacgag gatctgctcc gggcgaactg ggagtgctaa
     4441 gcggagggac aagagtcgga atctcgtcga tcgaatgggg cgaacgcgac accggctttt
     4501 ctactttccg ggcgatgccc gctcaaggag tgcagctcaa gaaggaggat catatgcagc
     4561 tgactttggc acctcaggag caggtggtat tgtcctgggc ggtcgagagt gcaatctcgg
     4621 acctcggtac tgagatcggt cacaccgaca accaggccat gcggcaagac ttgcaagagc
     4681 gaaagggaat cctcttaacg attcttggcc ggctcagagt cactacctga gagcgtctgc
     4741 tctccgcacg cgagcgttcc agacttcagg atgaggagat gccgtggtga tgaaccgcgt
     4801 cccgtcgtcc ctcggccatg atcgatccag aaggtaagcg gaacgtccgt ccgtgatcga
     4861 ttgtccggtg gattagggaa aaatcttcga atgaccgagt gtctgaagcg tgtgtcttag
     4921 gccgttctct ttaagcctgt ctggatcgcg tgcaatgcct tcacgccctt tcacgaagga
     4981 gaatcacatg ttcgtgtacc tgctggtttt tgcaatcgtc attggatttg ctttttcagg
     5041 cttcgctcaa ttcgtggata aaggcgagta tgtcgaggtg gcgcaaggca cgaagctttg
     5101 tcctgacgtc atcctggaaa aatcgaaagg gaggcttgat ccagagaacg tctattggcg
     5161 agtcaccagt aaggtacgaa cgaatggcac acagaccgtc gccgcatttg tccttgaaac
     5221 cctgtacgac cgcaaaagcg gtgagaccac gatcaaggaa cgtcgatatg acgaggacta
     5281 tgccatgctc tttaagagat ccccggagca caaagggatg cttctcatga tcagcccgaa
     5341 ggacggtcga gtggaagcga cggtggagat ttgcaggaag aagaaatagc gtgtctgccc
     5401 tcgacgcctc ttatcgatca gagcgctcca tacatgacgc atgcagtgtg gccagctggg
     5461 aacaggtaga ggggcgatac ccgcctatga aaattaacca caaatggtta atagttgaaa
     5521 ttaaaagtag tttaaagata tacagcttga ctatgatcgg taaacttcgt agagtaggtt
     5581 atggaagcag gacaaacctc tctaagtgaa gagttgttga ttcttgggcg ctatgaaagt
     5641 taggaacgta atgttggcgt gtaggctcaa gctctgacgt ttcagtttcg ctgactgtcc
     5701 catcctttgc gcttcccgca tccttcccag cgcgatcgat ttctcagtct ccgccgcttc
     5761 aatgccagag tgccagtgaa gtcgggacga tctcgatagg tctccgatgg ctggttgtcg
     5821 cagacgtcag tccgtcggcc tgccgatgta gtgatttccc ggtcgctgat cggggcgcta
     5881 tctgtcggag tgattgaggc gtgtgcatcg aagaggaggc tttcatcatg agattcatca
     5941 tgatcgcaat cgtagggtac gtgtttttag ggatgaccgt acttgctctg gcaaatcctg
     6001 cgatgctgcc gaagcatccc ggatatccgt cgagcggaga atttgcgaac gatacgggac
     6061 aaaaaaactt aacctatcgc cagtctatcg aggaggctgc gaagtccgga gatacaacca
     6121 tgggcacg
//
LOCUS       DATNDD010000025         3038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1408924, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000025 DATNDD010000000
VERSION     DATNDD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408924"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /gene="polA"
                     /locus_tag="VN647_00620"
     CDS             <1..1473
                     /gene="polA"
                     /locus_tag="VN647_00620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWG95554.1"
                     /translation="KSQGIKLIADYLLNPNRRDHQLDTLALELLDHRLGTQAKEPSPP
                     ASLFQVETASQEQTAERAAVIAKITHVMFDRLRDQGSLRLFQEVEMPLVPILADIEQN
                     GFLLDVDGLHALSKELERELDRMMDTIARLAGGEFNINSPKQLATVLFERLGLKPVRK
                     TKTGYSTDEDTLTQLAAQHELPAQILNYRSLSKLKSTYVDALPELVHPETKRLHTSLN
                     QTVAATGRLSSTDPNLQNIPVKGDYGLRIREAFIVPKGHELLCADYSQIEPRILAHLS
                     QDPRLLSVFTKAEDIHMATAMEIFGLPSSQITRDMRRAAKTVVFGIVYGISPFGLSQN
                     LGVPQAEAKKYIETFFERFPAVRALMDRNIAEGREKGYTTTILSRRRPIPELQSSDPI
                     QRGIGERMAVNSPIQGSAADLIKVAMINVHKKLHEELPHVKMILQVHDELIFEVPDHD
                     LEEAKRLVRQGMEGVGEQLGLSVPLKVDLGIGKNWRVAHP"
     gene            1814..2023
                     /locus_tag="VN647_00625"
     CDS             1814..2023
                     /locus_tag="VN647_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWG95555.1"
                     /translation="MSKYEVIIYWSKDDQAFIAEVPELAGCAADGATYQEAQANVETV
                     ISEWIETAKELGRPIPEPKGRLAFA"
     gene            2471..2728
                     /locus_tag="VN647_00630"
     CDS             2471..2728
                     /locus_tag="VN647_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95556.1"
                     /translation="MLTIRISVAWLIAVICLGTLGLLLLLVQPFTVKAQEPKRFQYQI
                     VEVIPDTETMQTRLNEFGSNGWELVGFAMGNMTNPRMIFKK"
BASE COUNT          785 a          791 c          815 g          647 t
ORIGIN      
        1 aagagccagg gtatcaagtt gattgcggac taccttctaa atcccaatcg acgggaccat
       61 caacttgata ccctggctct tgagctcctc gaccatcggc tcggtactca ggcgaaagaa
      121 ccatctccac cggcatcgtt gttccaagtg gagaccgctt cgcaggagca aaccgcggaa
      181 cgcgcggcgg tgatcgcgaa gatcacgcac gtaatgttcg atcggctgcg agatcagggg
      241 agtctgagac tgttccagga agtcgaaatg cccctcgtac cgatcctggc agacatcgag
      301 cagaacgggt tcttactcga tgtcgacggg ttgcacgcgc tgagcaagga gctcgaacgg
      361 gagctcgacc gcatgatgga caccatcgcc cggttagccg gcggcgagtt caacatcaat
      421 tcgccgaaac agctggcgac agtgctcttc gaaaggctcg gcctgaagcc ggtccgcaaa
      481 actaagaccg gttactccac cgatgaagat acgctcacac aacttgcggc acaacatgag
      541 ttacccgcac aaatactgaa ttatcgaagc ctcagcaagc tcaagtcgac ttatgtggat
      601 gcgttgccgg aactggtgca tccggaaacc aaacgtctcc acacttcgct gaaccaaacc
      661 gtcgccgcaa cgggacggct ctcgtccacc gatcccaatc tccaaaacat ccccgtgaaa
      721 ggcgactacg gactccgcat ccgcgaagcg ttcatcgttc ctaaaggcca tgaattgctc
      781 tgtgccgact acagccagat cgaaccgcgt atccttgccc atctttcgca agatccgcga
      841 ttgctgtccg tttttaccaa ggcagaagac atccacatgg cgacggccat ggaaatcttc
      901 ggcctgccgt cgagtcagat tacaagagat atgcgccgcg cggctaagac cgtcgtcttc
      961 ggcatcgtct atggaatcag tccgttcggc ctttcccaga atctcggcgt accccaggct
     1021 gaggccaaaa aatacatcga aacctttttt gaacgattcc cagccgtccg tgccctgatg
     1081 gatcggaaca tcgccgaagg acgggagaaa ggctacacca ccacgatcct tagccgccgc
     1141 cgcccgattc ctgaacttca gagtagcgat ccgatacaac gtggcatcgg cgagcgtatg
     1201 gcggtaaata gtcccatcca aggttccgcg gcagacttga tcaaggtcgc tatgattaac
     1261 gtccacaaga aactccacga agagctgccg cacgtgaaaa tgatcctcca agtccacgac
     1321 gagttgatct tcgaagtgcc ggatcatgat ctggaagaag cgaagcgact ggtgaggcaa
     1381 ggaatggaag gcgtcggcga acagttaggc ttgtcggtgc ctcttaaagt ggatctcggc
     1441 atcggcaaaa actggcgagt tgcgcatccg taggcaagta agcagttatt ttgacaggat
     1501 agtgggacga ctatagaatt tgacaggtcg actgcttctt tccttactca gtagaagatt
     1561 gagtgacacg atctgataag ctacttgagc gtgggcttcg gggaacatcg gacgcaaaca
     1621 tttcgtttga tgggttgctg cacttgctgg tggagttggg ttttacagag agttcgaggt
     1681 agtcatcata tctttacaag aatgggagtc gaagagattt tgaatctaca gccaaaaggc
     1741 tcccacgcca aaccgtatca ggtgaaacaa gtacgccagg tgatccttcg gtacaagctg
     1801 gggggcgacg tcaatgagta agtacgaggt catcatctat tggagcaaag acgatcaagc
     1861 cttcattgcc gaagtgccgg agttggcggg ctgcgcagca gatggcgcca cgtatcaaga
     1921 ggcgcaggca aacgttgaaa ccgtcatcag tgagtggatc gaaacggcaa aggaactggg
     1981 gcggccaatc ccagaaccga aagggagatt ggcatttgcc taagatttga aagattctcg
     2041 acactactaa caatatggcc atttgcagta gaaaccatat caaggaggtt cgacggaagt
     2101 gagatggaac gtttcgagac cggacccatt tggtacctag gcttgtattt tatgagtatc
     2161 cagcctgcgg tgagaaagtc tatgaccgcg aggcaatgcg caaaatagag gcgcattccg
     2221 cctgctctta ccagagctcg cgcctcctag cctacataag ccgcccgata aaccgtttca
     2281 tgcagtccct gtcgaagggt atactttttc gcgagaacca actatctcac cggcagctca
     2341 aacccaaccc ctcacagctt acgcttgcaa caaagacacg cagccagtaa aaacagagat
     2401 ggagaatctc ggcgtcggca agaaccggct cgtcgcactc tcctaactca ttaccaatgc
     2461 gaggtgatat atgctgacca tacgaatttc tgtcgcgtgg ctgatcgcag tcatctgcct
     2521 tggaaccctt ggacttttgc tgctccttgt tcaaccattc acggtcaagg cccaggagcc
     2581 gaaacgattt caataccaaa tcgtcgaggt gattcccgat actgagacta tgcagaccag
     2641 actgaatgag ttcggctcca acgggtggga gctggtaggg ttcgcgatgg gaaacatgac
     2701 gaatccgagg atgatcttta agaagtgacg cccagcggct ggtggtgcac aaaacagaag
     2761 cggtggtaag cagcctggcc tgttggtcct gcacatcgtg aatctcggtg tcggtaagaa
     2821 attggtgagt ggcgcatcca tgaggacggc aaaattgacg aataggtatg aaatgcccct
     2881 attcttgtct taaaaagatc tcatgcagca cttgtcgatt catgacagag ctcgcttata
     2941 aagtcgtaga gaggggtgag ccttgtttag tgatgcatgg cgggctgggg ttcgatcata
     3001 cgatgctgca tccgggattg aatcgccttg cagtcact
//
LOCUS       DATNDD010000026         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1974107, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000026 DATNDD010000000
VERSION     DATNDD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974107"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VN647_00635"
     CDS             complement(<1..266)
                     /locus_tag="VN647_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HWG95557.1"
                     /translation="MYLKRRGKVPNQAHVGIPEGLYEEEHGRNGFAGAVSHLYRTHPP
                     TSWITIEGKLRPRAFACTQIPEAALSNASQSVLMMQSQDVGLYMS"
     gene            complement(297..890)
                     /locus_tag="VN647_00640"
     CDS             complement(297..890)
                     /locus_tag="VN647_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWG95558.1"
                     /translation="MKQTIGFDHVTLCVDNLEAAEFLFAKILGFEVIWSAEDVGSNTS
                     SMDTVVVQCGAVKIALMQGRDKAGKSQINQFIEKYGQGVQHVALEVDNIEDVCREWEA
                     HGVRFSGPVKEGRDGFGPLRQRFTYPLFPNSGLFIELTQRQHGEEAARTFVRSTVESL
                     YKDIERDQTSGIERTIVDYDSSSMPKQKQKEPLKKAI"
     gene            1141..1878
                     /locus_tag="VN647_00645"
     CDS             1141..1878
                     /locus_tag="VN647_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWG95559.1"
                     /translation="MTFHQELRRRVLEHGAINNSYLERFRVGDLTDSDLKEFALEFYN
                     FSRFFPRILMSQLVNTEDERVADELTKVLYSELGDGQTRHRHELLYRNFLRSVDIDIH
                     EAMTRPMFPSTHAYIEGMERLYTDGNHAKALGASFGLENMAITMWDHLIPGLTHLKLT
                     RCPKMDLTYFTFHRQLEVTHEEAMEQAVAAVQGTDETRMSKQDEDDFRSGVDAVLDYL
                     EGFWAGLEQRRRNPGNTWTGLAQRRAG"
     gene            1932..3647
                     /locus_tag="VN647_00650"
     CDS             1932..3647
                     /locus_tag="VN647_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWG95560.1"
                     /translation="MQQTWISRYDSGVPASINYPAWTVSDLLQHSSERFANSPALMFY
                     GTCITYGELDGLATRFALGLRQLGVGQGDRVATMLPNIPQAVIAYYGILKAGAVVVPT
                     NPLYVEREIQTQLTDAGAETMVVLDLLYLRVRAVRDATTLPTRIIVTGVQDYLPLFKK
                     LLYPIKARLAKRWVSVEKSPPVYDFLELLCINSVNGADHSAGLPLVRPTDLAQLQYTG
                     GTTGTAKGVMLTHRNVVVNTLQGRYWSPDFREGQEIFLGAIPFFHCYGQATCQNLAIA
                     TGCPIVLLSRFHANEVIKAIHTHRITMFSGVPMMYSMIVEHPDVGRYDLRSLRVCLSG
                     ASSLPTDVQERFENLTGARISEGYGLTEAGPTTHCNPLHGEHPRGSMGLPFPDTEARV
                     VDPDAGLEEVPKGETGELIVRGPQVMRGYWSREDETRAVLRGGWLHTGDLVRRDDRGY
                     FYFVDRKKDIIKSRGETVYPREVEEVLRQHPAVSEAAVIGVADHDYGEVIKAYVVAKP
                     GLLITEQALITHCAGLLARYKIPSIVEFRQELPRTVIGKVLRRVMRAEAAQASAEEQL
                     QRQAV"
     gene            3668..>4300
                     /locus_tag="VN647_00655"
     CDS             3668..>4300
                     /locus_tag="VN647_00655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWG95561.1"
                     /translation="MRDVVIVAGVRTPIGNFGGALKDVPPQRLGELVVREVLARGGLD
                     SRLIEEVIFGCVGQPSDAFNIARVIALQAGLPLTTPAYTVQRNCSSGLQPFVNAFQNL
                     QSHDADVQIVGGVESMSRAPFLSRDMRWGKRLRHAEFIDSIWEGLTDPVCGQLMGRTA
                     ETLAEELGITREEQDRFAVESHHRAFKAVREGRLKGEIHPVTVSKSVGGRE"
BASE COUNT          947 a         1188 c         1184 g          981 t
ORIGIN      
        1 gacatgtaga gaccgacatc ctggctttgc atcatcagca ctgattgcga ggcattggat
       61 aatgcagcct cgggaatctg ggtgcaggca aaggccctcg gcctcagctt tccctcgatt
      121 gtgatccagg aagtcggtgg atgtgtccga tagagatgtg aaaccgctcc ggcaaatccg
      181 tttcgcccat gctcctcttc ataaagccct tcaggaatgc ccacatgagc ctggttggga
      241 acttttcctc tcctcttgag atacatggcc tgttccccct ggcggattgg ccgaggttaa
      301 atcgcctttt tcagcggctc cttttgcttt tgtttcggca tggaggatga atcgtagtcc
      361 acgattgtcc gttcgattcc tgaggtttgg tctcgttcga tatctttgta caacgactcc
      421 accgtgcttc ggacaaacgt tctcgccgct tcttcgccat gctgtcgttg agtcagctca
      481 atgaacagtc cactgttcgg gaaaagagga taggtgaagc gttgccgaag cggcccaaac
      541 ccatcccgcc cctctttgac tggtccacta aacctcacgc catgtgcctc ccactctcga
      601 cacacatcct cgatgttgtc gacttccaac gcaacatgct gtacgccctg tccgtatttt
      661 tcgatgaatt ggttgatttg agactttccc gccttgtctc gcccctgcat gagagcgatc
      721 ttgacggctc cacactgtac gacgacagtg tccatcgatg aggtgttact tcccacatct
      781 tcagccgacc agatcacttc aaagcccaag atcttggcga acaggaattc tgcagcttca
      841 agattgtcca cacagagggt gacatgatca aaaccgattg tctgtttcat ggggcctcca
      901 catcgcccac atcttgctcc gaggaacctt ttccggaatt ataatttata attgtatgat
      961 gcgataatac tattattaaa atatcattac aagacgcttc tcgattaatt tgtcatatat
     1021 tgagtcgttt cattataatt tatcggaaca tcgtcgatca tcgctctatt gcaattgtaa
     1081 tattaaaatt atattatgtg ttcacataaa tctccatcga gaggacactc ggaggtacct
     1141 atgacgtttc atcaagaact gagacgacgg gtgctcgagc atggcgccat caacaactca
     1201 tacttggaac gcttccgagt aggagatctc accgactccg acctcaagga attcgctctc
     1261 gagttttaca acttttcccg gtttttcccg agaatcctga tgtctcagct ggtcaatacc
     1321 gaggacgagc gggtggccga tgaactgacc aaggtcctgt actccgaatt gggcgacgga
     1381 caaacccgtc atcggcatga actcctctat cgaaactttt tgcgatccgt ggacatcgac
     1441 atacacgaag ctatgacacg accgatgttt ccctcgaccc acgcctatat agagggaatg
     1501 gaacgactct acaccgacgg caatcacgcc aaggcgctgg gagcctcctt tggactcgag
     1561 aacatggcca tcaccatgtg ggatcatttg attccgggtc tgacccacct gaaactcacg
     1621 cgttgcccca agatggacct cacctatttc acgtttcacc ggcaacttga agtcactcac
     1681 gaagaggcaa tggaacaggc ggtagcggcc gtgcaaggaa cggatgagac cagaatgtcc
     1741 aaacaggacg aggatgattt tcgttccggc gtcgatgccg tgctcgacta ccttgaaggg
     1801 ttttgggcag gtttggagca acggaggagg aaccctggca acacgtggac cggcttagct
     1861 cagcggcgtg cgggttagcg tcgtgagcga tacggctggt ttgcatgtgc gtgatggtgg
     1921 aggagggtcc tatgcaacag acttggatca gccgatatga ttccggggtg cccgcaagca
     1981 tcaactaccc ggcatggact gtatcggacc tcctgcaaca ttcatcggag cgtttcgcga
     2041 actccccggc actgatgttt tatggtacct gcatcactta tggagaactg gacggcctcg
     2101 cgactcgatt tgcgcttggg ctgcgacaac tcggcgtcgg tcaaggcgat cgggtcgcca
     2161 ccatgcttcc aaatattccg caggcggtca tcgcctatta cggaattctg aaagccggcg
     2221 ccgtcgtcgt gccgaccaat ccgctctacg ttgaacgaga aattcagaca caactgaccg
     2281 atgcaggggc ggaaacgatg gtggtccttg acctactcta tcttcgcgtt cgagcggtga
     2341 gagacgcgac gacgctcccc acgcggatca tcgtcaccgg cgttcaggac tatctccccc
     2401 tcttcaagaa actcctctat cccatcaaag ctcggttagc caaacgttgg gtttccgtcg
     2461 aaaaatcgcc gcccgtatat gacttcctcg aactcctctg catcaactct gtaaacggtg
     2521 cagatcattc cgccggtctg cctctggtac gaccgaccga tctcgcacaa ctgcaatata
     2581 ccggtgggac gacgggaaca gccaaaggcg tgatgctcac gcaccgtaat gtggtcgtca
     2641 atacgctgca gggccgatac tggtcacctg attttcgtga aggtcaggaa atctttctgg
     2701 gtgcaattcc atttttccac tgctatggtc aggccacctg tcagaaccta gccatcgcga
     2761 cgggctgtcc aatagtcctc ctctctcggt tccatgcgaa cgaagttatc aaggcgatcc
     2821 atacccatcg cattaccatg ttttcgggcg tgcccatgat gtattcgatg atcgtcgagc
     2881 atccggatgt cggccgctac gacttacgct cgttgcgggt gtgtttgagt ggggccagct
     2941 cgcttcctac agatgttcag gaacggttcg aaaatctcac cggggcaagg atttcagagg
     3001 ggtatggcct gaccgaagcc ggtcccacga ctcattgcaa tccgttgcac ggagaacatc
     3061 cacgaggctc gatgggactt cccttccccg ataccgaggc gcgggtggtg gaccctgatg
     3121 ccgggctgga ggaggtaccg aagggtgaaa caggcgaatt gatcgtgcgc gggccacagg
     3181 tgatgcgggg gtactggagc agggaagacg aaacacgcgc cgtgcttcgt ggcggatggc
     3241 tgcacaccgg cgacctcgtc cgccgggacg atcgcggata cttctacttt gtcgatcgca
     3301 agaaagacat catcaagtcg cgaggagaaa cggtgtatcc gcgagaagtc gaagaagtct
     3361 tgcgtcagca cccggcggtg agcgaagccg ccgtgatagg cgttgcggac cacgactacg
     3421 gtgaggtcat caaagcctac gtcgtggcga agccgggatt gctcatcacc gagcaggcat
     3481 tgatcacgca ctgcgccggc ttgctggcgc gatacaagat cccgtcgatc gtggaatttc
     3541 gtcaggagtt gccgcgaacg gtcatcggta aggtgctgcg cagggtaatg cgtgccgagg
     3601 ctgctcaagc gagtgccgag gaacagcttc aacggcaggc tgtgtgaacg ttcggatgga
     3661 ggaccctatg agagacgtcg tgatcgtggc cggtgtgcga acgccgatcg gcaatttcgg
     3721 cggtgcgttg aaggacgtcc cgcctcaacg gctcggggaa ctggtcgtcc gcgaagtcct
     3781 cgcacggggc ggtcttgatt ccaggctgat cgaggaggtc atctttggct gcgtcggaca
     3841 gccgagtgac gcattcaaca tcgcccgcgt cattgccctt caagccggac ttcccctcac
     3901 gactccggcg tacaccgtgc agcgcaactg ttcctcggga ctccaaccgt ttgtcaatgc
     3961 cttccagaat ctccagagtc acgatgcgga cgtgcagatc gtcggcggcg tcgaaagcat
     4021 gagtcgcgcg ccgtttctgt ctcgggacat gcgatggggc aagcggcttc gacatgctga
     4081 attcatcgac agcatttggg aaggcttgac ggacccagtt tgcggccagc tcatgggacg
     4141 gacagcggag accttggccg aagagttggg gatcacacgg gaagaacagg atcggtttgc
     4201 ggtcgagagc caccaccgtg cattcaaggc ggttcgcgag ggcaggctga aaggggaaat
     4261 ccaccctgtc acagtctcca aatcggtcgg ggggcgagag
//
LOCUS       DATNDD010000027         3854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3994340, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000027 DATNDD010000000
VERSION     DATNDD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3994340"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VN647_00660"
     CDS             complement(<1..1407)
                     /locus_tag="VN647_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HWG95562.1"
                     /translation="MTIPGFPRRQGLYDPQHEKDSCGIGFVVNIKGKKSHDIVRKGLQ
                     VLENLTHRGAQGSDPYTGDGAGILLQVPHTFLKRVAGDAGLSLPEVGEYGVGQLFLPP
                     NADSRRLCEKLFTEILVEEGLRLLGWRDVPVKSDQIGAQARTTEPFMRQIFIARDALN
                     EARFERKLYVIRKRVEKAVAESAIQGREHFYVSSLSAYTIVYKGLLLPHQMSAYYQDL
                     TDERMVSALALVHSRFSTNTFPTWPRAHPYRYVCHNGEINTLKGNVNWMKARQGRLHS
                     ELFGNDMEKLFPIVSENQSDSACLDNTLEFLLLGGRSLPHAMMMLIPEPWVANAQMDL
                     DRRGFYQYHAAMMEPWDGPAAVCFTDGKMIGATLDRNGLRPCRYQVTTDGLVVLASEA
                     GVLPADAKDIRMKGRLQPGRMFLVDTVQGRILDDEEIKADIVGRKPYRSWVTQYGVSL
                     DELPDPLNVPQPDHPTIRQ"
     gene            1627..2418
                     /locus_tag="VN647_00665"
     CDS             1627..2418
                     /locus_tag="VN647_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWG95563.1"
                     /translation="MAQGPAIATLHSMSDVWDAYRGELDGVEDRVKKNLDSSVTLVNT
                     VAAHILSGGGKRIRPLLLLLSARLCGYPGTEHHQLGSIVEFIHTATLLHDDVVDDADL
                     RRGRRTARKVWGNQISILVGDYLYTKAMCKVVEFQSQGINEVLAEACKKMAEGEVLQL
                     YYNGNPSMPEIDYIKIVEHKTAGLIAAACRMGAILAEASETQQMALFRFGQYLGIAFQ
                     VADDTLDYIANGESLEKQSARISGKEKRRYPCCICYSIARSRIAK"
     gene            2415..2630
                     /locus_tag="VN647_00670"
     CDS             2415..2630
                     /locus_tag="VN647_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95564.1"
                     /translation="MIKDRMETRTLSTADLERILLLMADFGSITYAMDRASAYIAAAK
                     HELERFDDSTARRALSVTADYMITRDR"
     gene            2757..>3854
                     /locus_tag="VN647_00675"
     CDS             2757..>3854
                     /locus_tag="VN647_00675"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HWG95565.1"
                     /translation="MSQIFPFQGMLYDQTLVGSIKDVVAPPYDIIDAEGQKRLHDRHP
                     HNIIRMELGLNQTGDSPAHNRYTRAAATLQTWLNEGILKRDAQPAVYFHTIEYTPPPS
                     SALDAPKMTLRGFLALAKLETLDSGHIYPHENTRAAAKTDRLNLIEACRSNFSPIWSL
                     YSDPLGAIIQLLETAVKGKPARYDFQDDAGCRECLWGVTDETVIQQITAAMQTKPLFI
                     ADGHHRYETALNYQKLRRQQAGPQTGLQPYDSVLMLLTPLEDPGLTVLPTHRVATTPL
                     PSSDQIRTVLGDAFEFQEFPFSASTQQQARSQFLTSLRTEGKNTPVFGLARQGDDRYV
                     TLTLKPAHRPSPQASPRAKLDVSLLQQLIVTR"
BASE COUNT          855 a         1159 c         1030 g          810 t
ORIGIN      
        1 ctggcgtatc gtcgggtggt cgggctgcgg cacattcagc ggatccggca actcatccag
       61 cgagacacca tactgcgtca cccaactgcg atagggcttg cggccgacga tatcggcctt
      121 aatctcctcg tcatcgagga tacggccctg caccgtgtcg acgaggaaca ttcgacccgg
      181 ttggagtcgg cctttcatgc ggatatcttt cgcgtcagcc ggcaagaccc ccgcctcgga
      241 tgccaggacc acgagcccgt cggtcgtcac ctgatagcgg cagggacgga gtccgttccg
      301 gtccaacgtc gcgccgatca tcttgccgtc ggtgaaacag acggctgccg gcccgtccca
      361 cggctccatc atcgccgcgt gatactgata gaagccccgg cgatccaaat ccatctgcgc
      421 atttgccacc catggttctg gaatcagcat catcatggca tgcggcagcg aacggccccc
      481 gagcaacaga aattcgaggg tgttgtccag acaggccgag tcgctttgat tctcggagac
      541 gatcgggaac agcttctcca tgtcattccc gaacagctcg gaatgaagac ggccttggcg
      601 ggccttcatc caattgacat tccccttcag tgtattgatc tctccgttgt ggcacacata
      661 ccgataggga tgggcccgcg gccaagtggg aaaggtattc gtgctgaaac gtgagtgcac
      721 caaggccagt gcgctcacca tccgttcatc ggtaaggtct tggtagtaag ccgacatttg
      781 atggggcaac agcagtccct tgtagacaat ggtgtacgcc gacaagctgg atacgtagaa
      841 gtgttctcgc ccttgaatgg ccgactctgc cacggccttt tcaacccgtt tgcggatcac
      901 atacagcttt cgctcgaacc gggcctcatt caaggcatcg cgagcgatga agatttgccg
      961 catgaacggt tcggtcgttc gcgcttgggc accgatctga tcactcttga ccggcacgtc
     1021 gcgccagccg agcaagcgca ggccttcctc cacaaggatc tcggtgaaca gcttctcgca
     1081 cagtcgtctg gagtctgcgt tgggcggcaa aaacaactgc cctactccgt actcgccgac
     1141 ttccggcaat gacaggccgg catcccctgc gacccgcttg aggaatgtgt gcgggacctg
     1201 caaaagaatt ccggctccat ctcccgtgta aggatcactg ccttgcgcac ctcgatgggt
     1261 aagattctcc aacacttgaa gtcctttgcg gacaatgtca tgggatttct tccctttaat
     1321 attgacgaca aagccgatcc cgcaagagtc cttctcgtgt tgaggatcgt agagcccttg
     1381 tcgacgggga aacccaggaa tagtcatgta cctatctcgt tcaaagaaat gagagtttgc
     1441 ctagacatgg gggatagaac caatgcggac cttgggtgga ttttccccca tagccctcag
     1501 ggtgaacctc acccgattgt taggatggaa acttactgga tgggtcattc ttctgtcaag
     1561 gttacgaggc ttataccgct ttcaattgct tgactttatt tcctcggctg ccataccatc
     1621 cgccgcatgg cacaaggtcc agcgatcgca accctgcaca gtatgtcgga tgtgtgggac
     1681 gcctaccgtg gtgaactgga cggagtggaa gatcgagtca agaagaacct cgactccagc
     1741 gtgaccctag tcaatacggt cgccgcccac atcttgagcg gtggcggcaa acgcatcaga
     1801 cccctgctcc tcctcttgtc ggctcgtctc tgcggttatc ctgggaccga gcaccatcag
     1861 ctcggcagta tcgtggaatt catccatacc gccaccctat tacacgatga tgtcgtcgat
     1921 gacgccgatc tccggagagg cagaagaacc gcgcgcaagg tttggggaaa ccagatcagc
     1981 attctggtcg gcgactacct ctataccaag gccatgtgca aagtcgtcga gttccagagt
     2041 caaggcatca atgaagtcct agccgaggcc tgcaagaaaa tggcggaagg tgaggttctt
     2101 cagctgtact ataatgggaa tccttcaatg ccggagatcg attatatcaa gatcgtcgag
     2161 cacaagaccg ccggcttgat tgccgcggcc tgccgcatgg gggccattct ggccgaggct
     2221 tctgaaacgc aacagatggc gctgttccgc ttcggccagt acctcggcat cgcctttcaa
     2281 gtcgccgatg ataccttgga ttacatcgca aacggcgagt cactggaaaa acaatcggcc
     2341 aggatctccg gcaaggaaaa gcgacgctac ccctgttgca tctgttacag cattgctcgg
     2401 agcaggatcg ccaaatgatc aaggatcgaa tggaaactcg gacactcagc accgccgatc
     2461 tcgaacgaat cttattgctg atggccgact tcggttcgat tacctatgct atggatcgcg
     2521 caagcgccta cattgccgcc gccaaacacg agctcgaacg cttcgacgac agtactgcgc
     2581 ggcgcgcgct gtcggtcacc gccgattata tgattacccg cgaccggtag ctgcgttcct
     2641 gccagctgga agggcgctgg gccgcaggag cggcacggcg tagggcgaaa ggatcccact
     2701 ctctcctgta accatcaagc cgtcgtcgct gtcacgacat tgccaagggg ttgttcatgt
     2761 cacagatctt tccgtttcag ggcatgctgt acgaccaaac gctcgtgggc tccatcaagg
     2821 acgtggtcgc tccgccatac gacattattg acgcggaggg acagaaacga cttcacgatc
     2881 gccatcccca caatattatc cgaatggaat tggggcttaa ccagaccggt gacagccctg
     2941 cccacaaccg gtatacccgc gcggcagcca cgctgcagac ctggctcaat gaaggtattc
     3001 tcaagcgtga tgcacaaccg gccgtctact ttcacacgat tgaatacact cctcctccct
     3061 cctcagccct cgacgctccc aagatgacgc ttcggggatt cttggcgctg gcaaagctgg
     3121 agacgctcga ctccgggcat atttatcctc acgagaatac acgcgccgcg gcgaagactg
     3181 atcgcctgaa tttgatcgaa gcctgtcgct caaacttcag cccgatttgg tctctctact
     3241 ccgatccact cggtgccatc atccaacttc tggaaactgc ggtaaaaggc aagccggcgc
     3301 gttacgactt tcaagatgat gcgggctgtc gcgaatgcct gtggggcgta accgatgaaa
     3361 ctgtcattca acaaatcacc gctgcgatgc agaccaagcc gcttttcatc gcggacggtc
     3421 accatcgcta tgaaacggcg ttgaattatc aaaaactccg gcgacagcag gcgggccctc
     3481 aaaccgggtt gcaaccttac gactcggtgc tcatgctgct cacccccctt gaagatccag
     3541 gattgacggt attgccgaca catcgagtgg cgaccacccc cttgccttct tcggaccaga
     3601 tcaggacggt actgggagac gcgttcgagt ttcaagaatt ccccttcagt gcatccacac
     3661 aacagcaagc gcggtcacag ttcctgacga gcttgcggac agaagggaag aataccccgg
     3721 tgttcggact ggcgcggcaa ggcgacgacc gatacgtcac gctcacgctg aagccggccc
     3781 atcgaccttc ccctcaggca tcccctcgag ccaagctcga cgtctccctg ctgcagcaac
     3841 tgatcgtgac cagg
//
LOCUS       DATNDD010000028         2761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1722601, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000028 DATNDD010000000
VERSION     DATNDD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722601"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..689
                     /gene="rnk"
                     /locus_tag="VN647_00680"
     CDS             273..689
                     /gene="rnk"
                     /locus_tag="VN647_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HWG95566.1"
                     /translation="MESRDIYITKFDLVRLKELLEVGISFKERDRGYLESLQNELDRA
                     HIVEPSAIPDNVVTMNSQVRLKDMETGEEKSYTLVFPSDADIEKNRISILAPIGTAIL
                     GYRAGDTVDWLVPAGTRKVRIEEILYQPEAAGRYDL"
     gene            complement(811..1452)
                     /locus_tag="VN647_00685"
     CDS             complement(811..1452)
                     /locus_tag="VN647_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWG95567.1"
                     /translation="MHDRGTYAHNATALFVIANPIGVTPLFISLTNRQTEEQRRHTAH
                     LTATTVIIVLITAMFLGEAILHVFGIRMASFRAGGGILILLMAIAMLQARPSPTHHTA
                     EESKEAAVRDEVAVVPLGIPLVAGPGAISTVIIYADQAATWFDMAMLFLTVLCVAGSV
                     WIALHLSDRIRVMLGTTGINIVTRLLGLILAAVGVEFLADGLAQLLPGLANQK"
     gene            1533..2756
                     /locus_tag="VN647_00690"
     CDS             1533..2756
                     /locus_tag="VN647_00690"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWG95568.1"
                     /translation="MADNMNRPDQSPFILTINGGSSSIKFALFRAVDLSVPLTRGMVD
                     RIGLPNSELLVTDTATQQSSRRAVHAPDHRTCVGFITEWLEQQVGIGRLCAVGHRVVH
                     GGMTYSRPERVTPELLAELRRLCPYSPEHLPAEITLIESFRRYDPTLTQIACFDTAFH
                     RDIPRVARLLPIPRRYEKLGIQRYGFHGLSYAFLMRELTRMGAPGEAESRVILAHLGN
                     GASMAAVKNGQALDTTMGFTPASGLPMSRRSGDLDPGLVAYLARTEGMTVERFHGMIN
                     TESGLLGVSEISSDMRDLLKQERSDPRAAEAVGLFCYQARKWIGALAAALGGVDSLVF
                     SAGIGEHAPAIRARICAGLEFLGIVINEPANEANQPVISSEASRVRVRVIPTDEEREI
                     AESVVRLLGKENMDH"
BASE COUNT          601 a          715 c          847 g          598 t
ORIGIN      
        1 atcgccccag ggtgagaaat ctgtatgtcg cgaacggcca atcgtccatt acatcgccct
       61 gcttagtcgg atggacgaag atgcggatcc agctcgggca ggtgattcat gtctcggcat
      121 ttgtcttgtc ggcgaagagg ctgtatctcc ttggggacga tttgtgctaa gcattgaaat
      181 ataccgactc aactatgaca tatttgggcg gcatggcaga aaagattatg cggaccgccg
      241 gtcgcccggt gctgaacata aggagcatag acatggaatc tcgggacatt tacatcacta
      301 aattcgacct ggttcgcctg aaggaattgc tggaggtagg gatcagtttc aaggagcggg
      361 accggggata cctggagagt ttacagaatg agctggaccg ggcgcatatc gtagagccca
      421 gcgcaattcc tgataacgtg gtcacgatga attctcaagt tcgcctcaag gatatggaga
      481 cgggcgagga gaaaagttac accctcgtat ttccgtccga tgccgatatt gagaagaatc
      541 gaatctcgat tcttgctccc atcggcaccg ccatcctcgg ctaccgggcg ggtgatacgg
      601 tggactggct tgtgccggcc gggacacgca aggtacggat cgaagaaatt ctctatcagc
      661 ccgaggcagc cggacgttat gacttgtaac agtcgcacgt gaatcgtgcc gggatgtatg
      721 ggaggtggat ggcatctcaa ctgatataag cgtctcacgg tgaacgtcgg aagaacagcg
      781 ggcgctcgtt cgaaagatcg atcgaacgga ttatttctgg tttgcgaggc ccggaagcag
      841 ttgtgccagg ccatcggcga ggaattcgac tcccacggct gccagtatca atcccagcaa
      901 gcgggtgacg atgttgatgc ctgtcgtgcc caacatgact ctaatgcggt ccgaaaggtg
      961 cagcgcgatc cacaccgagc cggcgacgca gaggaccgtg agaaaaagca tggccatgtc
     1021 aaaccacgta gcagcctggt cggcataaat gatcacggtg ctgatcgcgc cgggccctgc
     1081 cacgagcgga atgcccaaag gaacgacggc cacctcgtca cgcactgcgg cctcctttga
     1141 ctcttccgcc gtgtgatgag tcgggctcgg tcgagcttgc agcatggcga tggccatcaa
     1201 gaggataaga attcccccgc cggcgcggaa tgaggccata cgaattccga atacatggag
     1261 gatggcctct cccaggaaca tggccgtgat aaggacgatg attaccgttg tggcagtgag
     1321 atgcgccgtg tgtcgcctct gttcctccgt ttgccggttg gtcaagctta taaaaagcgg
     1381 cgtgactccg atcggattgg cgatgacaaa caatgccgtg gcattgtgcg cgtaggtacc
     1441 ccggtcatgc atcgatacac tccttcgtcc gcgagtggat ggcggctgcg ggtgcgtctg
     1501 aactttagat ctccttgtaa tgcactgttg agatggctga caacatgaat cgaccggatc
     1561 agagcccgtt cattctcacg atcaacggag gatcgtccag tatcaagttt gctctatttc
     1621 gagccgtgga cctctccgtg ccgttgacgc gcggcatggt cgatcggata ggtctgccga
     1681 acagcgaact gcttgtcacc gatacggcca cgcagcaatc gagtcgtcgg gccgttcacg
     1741 caccggacca tcggacctgc gtcgggttta taaccgaatg gttggaacag caggtcggta
     1801 ttgggcgtct ctgtgccgtc gggcatcgtg tcgtccatgg tgggatgacc tatagcagac
     1861 ccgaacgagt gacgcccgag ttgctggcgg aacttcgccg actatgccct tacagccctg
     1921 agcatctgcc ggcggaaatt accttgatcg aatcgtttcg tcggtatgat ccgactctta
     1981 cccaaatagc ctgtttcgat acggcatttc accgggacat accgcgggtt gcgcgattgc
     2041 tgccgattcc gaggcggtac gaaaagttgg gtattcagcg ctatgggttt cacgggttgt
     2101 cgtatgcgtt tctcatgcga gaactgacaa gaatgggtgc gcctggcgag gccgagagcc
     2161 gtgtcattct agcgcatctc ggaaatggtg ccagcatggc ggcggtcaag aatgggcagg
     2221 cgctggatac gacgatgggc ttcacccccg catccggctt gcccatgagc cgtcgatcag
     2281 gcgacctcga tccaggactt gttgcctatc tggctcgcac ggaagggatg accgtggagc
     2341 gcttccacgg gatgatcaat accgaatcgg gcttgctcgg ggtctccgag atcagttccg
     2401 atatgcggga cttactcaag caagagcgga gcgatccacg ggcggccgag gcggttggat
     2461 tgttctgcta tcaggctcga aaatggatcg gtgcgctggc cgcggcgctg ggaggagttg
     2521 attccttggt cttcagtgcc gggatcgggg agcatgctcc cgcgatccga gcgcgaatct
     2581 gcgccgggtt ggaatttctg ggcattgtca tcaatgaacc cgccaatgag gccaatcaac
     2641 cggttatttc atccgaagca agtcgagtga gggtgcgcgt gatacccact gatgaagaac
     2701 gcgagattgc agagtccgtt gttcggctac tcggcaagga gaatatggat cactgagagg
     2761 t
//
LOCUS       DATNDD010000029         3681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3711132, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000029 DATNDD010000000
VERSION     DATNDD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711132"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..736
                     /locus_tag="VN647_00695"
     CDS             173..736
                     /locus_tag="VN647_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWG95569.1"
                     /translation="MILSSPLRLSTLWRHGFAIWLLAAVALGPNVEVSAASVEVWYGP
                     EDRPLEHLVRMYDRAQRYIFVAVYGLTSPITVKALVEAKRRGVDVRIVTDRERLEDAK
                     QRTALSTLHEAGIPIKINRHDALMHLKQAVIDDEINTNGSMNQTTSGNRYNDERLDII
                     RDHALAVKAREKFLSLWKDHERFQEWK"
     gene            840..2111
                     /locus_tag="VN647_00700"
     CDS             840..2111
                     /locus_tag="VN647_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWG95570.1"
                     /translation="MTEETDIAVVGAGGGGAVLALALAQKGIKTIVFEQAPGPPQGLR
                     GEILQPNGQQVLDRLGLLGRLPAESTRTVQKFHFCRVGGERLCTVDYSELPPPYDQAV
                     VTLPNVAHHAILNAIEGESSVSLRYRSAFTGLLRENGRVVGLTAKQGEDNVTVKAKVV
                     VGADGAFSKVREALPIPVDLHLYPQGYLIAIVEAAIPISEAKYFVGKRTILGMFPAAG
                     NKVYLFYMIKSGSYDRVKEQGIAALQESWIAIDPPSEAIFRTLIDWKQTAFMPTGRVR
                     TPTWVADGAVLIGDAAHAMNPHASQGRMQAMVDAMTLADLLPECLATNDYSAVILKRY
                     EDSRRPQVTMLQKLADEQVLFWNTANPIVAYLRDRVFRTLDRNARLRYRVLSTTAGLR
                     KEPPFGLMDRLMAAGLLPDYSVRDRAVGGIP"
     gene            2116..2793
                     /locus_tag="VN647_00705"
     CDS             2116..2793
                     /locus_tag="VN647_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95571.1"
                     /translation="MQSVDWTIDELPEETQKLLKSYVKDVAKVYGNELEGILLYGSAV
                     RGEFLPGRSNLNLILVMSSYDLSLLKKYDTIHKRWSKEQVVVPLFLTAGDLQSASQVF
                     PLEYQDILECHRLLWGQDPFVGLKIDVRYLAAEVLQALRGNLFRLRQRLVEGRSTEEA
                     MIILLSLSITALLPVLRGLQRLLLRPVLAHGEALLNDLESHLEINLTGLRDALLFKRG
                     HISPGQK"
     gene            2868..3620
                     /locus_tag="VN647_00710"
     CDS             2868..3620
                     /locus_tag="VN647_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWG95572.1"
                     /translation="MIPRSLRQKSGVPLLCLILVLWVDSAHASLYERPKERIPLPSPI
                     GYVSDHAQVVEREWKDRIRSVCIDLEKKSGVEMVIVTVPSIKPYPSAKHYADALYEKW
                     QIGSTQQEHGVMVLVAVQERQAAMALGRKMFPVITPTVRNEVGRDYLQPAIERGHFGE
                     GLYRTAVALATPAQEVRLNTPSRPRIKGLGIWITFGTTVAVVLFFWWLSRPDLRHPYR
                     RIQKGEYWGTGQGGFGGNWGGFGGGTSGEGYR"
BASE COUNT          818 a          953 c         1094 g          816 t
ORIGIN      
        1 tggatgtaag gaggcatgtg agtttgctcc agccgcttca agggcgctca catcagatca
       61 agcagctgct cacccaggag cagaaacagt ttctccgaca atggcttcag gtgcaggccc
      121 cacaggcctg ggaatcgtcc gaagaccact tccgcgatct cttcgagctg gaatgatctt
      181 gtcttcaccg ctccgattat ccacgttgtg gcgccatggt ttcgccatct ggctgcttgc
      241 ggcagtggcg ctcggcccaa acgtggaggt ttcagctgca tcggtagaag tctggtatgg
      301 cccggaggat aggccgctcg aacatctcgt acggatgtat gatcgcgccc aacgatacat
      361 cttcgtcgcc gtatacgggt tgacgtcgcc gatcacggtg aaagcgctgg tcgaggcgaa
      421 acgacgcggt gtggatgttc gtatcgtgac ggatcgcgaa cggcttgagg atgcgaagca
      481 acggacggct ctctcgacgt tgcacgaagc cgggattccg atcaagatca atcggcatga
      541 cgctctgatg catttgaaac aggcggtgat cgacgacgag atcaatacca acgggtccat
      601 gaatcagacg accagtggaa atcgttacaa tgacgagcga ttggatatca tcagggacca
      661 tgcgcttgcc gtcaaagcgc gtgaaaaatt tctttcgctc tggaaagatc acgagcgatt
      721 ccaggagtgg aaataataag aaggttgagg ttaaggtcga ggttcgtgaa ggtccggtgg
      781 aagggtaaga acgcgctcat gcccttagct tcaatcttgg cctattagag ggttgcgcgg
      841 tgaccgaaga aaccgacatt gctgtagtcg gagccggtgg aggaggagca gtgctggctc
      901 ttgccctggc ccagaagggg atcaaaacga tcgtcttcga gcaggcaccg ggaccaccgc
      961 agggattgcg cggagagatc ctgcagccga acgggcagca ggtcctcgac cgcttggggc
     1021 ttctgggacg attgccggcc gaatccaccc gtacggtgca gaagtttcat ttttgccgtg
     1081 tcggcggcga gcggttgtgc acggtggact atagcgagct gcctccaccg tacgatcaag
     1141 ccgtggtcac actaccgaat gtggcgcacc atgccattct caacgcgata gagggcgaat
     1201 cctctgtctc attgcggtat cgatcggcat tcacgggtct gcttcgagag aatgggcggg
     1261 tcgttgggct gactgcaaag caaggtgaag acaacgtgac cgtgaaagcg aaggtggtcg
     1321 ttggggccga tggagccttt tcaaaagttc gagaggcatt accgattccc gtcgatcttc
     1381 atctttaccc acaggggtat ttgattgcca ttgtcgaagc ggcgattccg atctcggaag
     1441 cgaaatactt tgtgggaaaa cggacgattc tcgggatgtt tcccgctgcc ggaaacaaag
     1501 tctacctttt ctatatgatc aagagcggtt catatgaccg tgtgaaggag caaggcatcg
     1561 cggcactaca agaatcatgg atcgcaatcg atccgcccag tgaagccatt tttcgtacgt
     1621 tgatcgactg gaagcaaacc gcctttatgc cgaccgggcg tgtccggacc cctacgtggg
     1681 tggcggatgg ggccgtgctg atcggggatg cggcgcacgc aatgaatcct catgcgtcac
     1741 agggccgcat gcaggcgatg gtggatgcga tgacactggc cgatcttttg cccgaatgtc
     1801 tggccacgaa tgactactcc gcagtaatac tcaagcgcta tgaagattcg cgaaggcctc
     1861 aggtcacgat gctgcaaaag ttggccgatg aacaggtgct gttctggaac acggcgaatc
     1921 cgatcgtcgc ctatctccgg gaccgcgtct tccgaacgtt ggaccgcaac gcacgactcc
     1981 ggtatcgtgt cttatcgacg acagccgggc ttcgcaagga gcctccattc ggtctgatgg
     2041 atcgcctgat ggcagccgga ttgttgccgg attattctgt acgtgacaga gcagtcggag
     2101 gcattccgta actccatgca atcggtcgac tggacgatag atgaactgcc cgaagagacg
     2161 caaaaactct taaagtctta tgtgaaggat gtcgcaaaag tctatgggaa tgaattggaa
     2221 ggaattctgc tgtacggcag tgcagtgcga ggggagtttt tgccgggccg ctccaatctc
     2281 aacttgatat tggtgatgtc gtcctatgat ctctcactgc tgaaaaagta tgacaccatt
     2341 cataagcgct ggagcaagga acaagtggtt gtccccttgt tcctgacggc cggcgatctc
     2401 caatccgctt cgcaggtctt ccctctcgaa tatcaagata tcctcgaatg ccatcgactg
     2461 ctgtgggggc aagatccctt cgtcggtctc aagatcgatg tccggtactt ggccgcagaa
     2521 gtcttacagg cattacgagg gaatctgttc cgccttcgtc agcgtctcgt ggaaggacga
     2581 agcaccgaag aagccatgat catccttttg tcgctctcca tcaccgccct cctgccggtg
     2641 ctgcgaggcc tgcaacggct gctgttgcga cctgtacttg cgcatgggga ggcgctcctg
     2701 aacgatctcg aatctcatct ggaaatcaac ctgaccgggc ttcgcgatgc gctcttgttc
     2761 aaacgtggac acatctctcc cggtcagaaa tagatcccca gactcatgga ccgctatctt
     2821 gaaagtctga caaaactcgt gactgccgcc gaagtgcgca tcacacgatg atacctcgtt
     2881 cgctgagaca aaaaagcggt gtgcctttgt tatgcctgat ccttgtgctg tgggtcgatt
     2941 ccgcccacgc ctcattgtat gaacggccca aagaacgtat cccgcttccc agccctatcg
     3001 ggtatgtgag tgatcatgct caggtggtgg agcgagagtg gaaggaccgt atcaggtccg
     3061 tctgcatcga tcttgaaaaa aaaagcggcg tagagatggt cattgtgacg gtccccagca
     3121 ttaaaccgta tccgtcggcc aagcactatg cggacgcgct gtatgaaaaa tggcaaatcg
     3181 gttcgacgca gcaggagcat ggggtgatgg tcttggtggc tgtgcaggaa cggcaggcgg
     3241 cgatggcctt ggggcgcaaa atgtttccgg tcatcacgcc gactgtcagg aacgaggtcg
     3301 gtcgcgacta tcttcagcct gccatcgaac ggggtcattt cggggaaggg ttgtatcgaa
     3361 cggcggttgc attggcgaca ccggcgcaag aagtacggct caatactccc tcacggcctc
     3421 gaatcaaagg actcggaatc tggattacgt ttggcaccac cgtcgccgtc gtgttgtttt
     3481 tttggtggct cagccgtccc gatctgcgac acccctatcg acgcatccag aaaggtgaat
     3541 actggggaac cggccaagga ggcttcggcg gaaactgggg cggcttcggc ggcggcacga
     3601 gcggggaggg atatcgatga ggatggaaaa tacatcaatg ccgcttgtcc tcgctacgct
     3661 cgttgtgttc ctggttggtt g
//
LOCUS       DATNDD010000030         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2858096, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000030 DATNDD010000000
VERSION     DATNDD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2858096"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /gene="mutS"
                     /locus_tag="VN647_00715"
     CDS             <1..1755
                     /gene="mutS"
                     /locus_tag="VN647_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWG95573.1"
                     /translation="LTPTTLLGVLDRTATAMGSRLLRQWLVRPLLRDDQIRFRLDAVE
                     ELKNHMQVRTALRTSLREIQDVARLGGRIVLGLAGPRELLALKQSLSVLPEIFDRLTP
                     LQSRLLCDARASWDNGQDLFDLIERAIRPDAPPSIRDGNIITEGYDPAIDELRKISRE
                     GKHWIAAIETQERDRTGIESLKIRYNQVFGYYIEITKANLARVPADYIRKQTLVNAER
                     FMTAELKDLEERVIGADAKLLACEQEVFTQLRSRLAKEAHRLDAMASVLSLIDVVAAL
                     ADTAALHRYTKPTVTEGGAITIREGRHPVVEGLCADSGFVPNDTVLDLETNRLLIITG
                     PNMAGKSTYLRQVALIVLMAQIGSFVPAAEAHIGLTDRIFTRVGASDNLAGGQSTFMV
                     EMVEAANILQNATQRSLILLDEIGRGTSTYDGLSIAWAIAEHIHDRARLGARTLFATH
                     YHEMTQLEQQRTGIKNYRVAVQECGGNVVFLRKIVAGKADRSYGIHVAKLAGLPTTVI
                     CRAQAVLSQLEQPESARSGLIQEQPPRTSEPLPQPHPLIEEVKQIDLFSITPLDALNR
                     LAELQRMVRSDSRDSSDR"
     gene            complement(1835..>2032)
                     /locus_tag="VN647_00720"
     CDS             complement(1835..>2032)
                     /locus_tag="VN647_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95574.1"
                     /translation="SALRLKDCAGKSEIDKACAAGKQDLNTSPVTPDGHNVGMQYDVM
                     LPGGKTLGGLIPWSAQYNRAH"
BASE COUNT          460 a          574 c          566 g          432 t
ORIGIN      
        1 ttgaccccta caactcttct gggtgtgctg gatcggaccg ccacggccat gggaagtcgc
       61 ctccttcgcc agtggctggt gaggccgtta ctccgcgatg accagattcg gtttcggctg
      121 gatgccgtgg aagaactgaa gaaccacatg caggtgcgga ctgcgttacg tacgagcttg
      181 cgggagatcc aagatgtcgc acgactcggc ggtcgcatcg tactcggttt ggccggcccg
      241 cgagaacttc ttgccctcaa acagtctctc tcggttttac ctgagatttt cgaccggctg
      301 acacccctcc agagccggct actctgtgat gcacgggcat catgggacaa cggccaagat
      361 ctgttcgacc tgatcgagcg agcgatccga cctgatgctc cgccctccat tcgcgatggg
      421 aatatcatca cagagggtta cgatccggca attgacgagc tgcggaaaat cagcagggaa
      481 ggaaaacatt ggatcgccgc aatcgagaca caggagcgtg atcgtacggg tattgaatca
      541 ctgaaaatcc gctacaatca agttttcggc tactatatcg agattaccaa agccaacctg
      601 gctcgtgtcc ccgccgacta cattcgcaaa caaaccctcg tcaatgccga acggttcatg
      661 actgcggaat tgaaggacct ggaagaacgt gttatcggag ccgatgccaa attgcttgcc
      721 tgcgaacaag aggtctttac tcagctgcgc tcccggttgg ccaaagaagc tcatcgattg
      781 gacgccatgg cctccgtcct ttcgctgatt gatgtcgtag ccgcattggc tgatacggcc
      841 gctttacacc ggtacaccaa acccacggtc acggaagggg gcgccatcac catacgggaa
      901 ggtcgccacc ccgtggtcga aggactctgt gccgattcag gattcgtccc gaacgacacg
      961 gtcctcgatc tggaaacgaa ccggctgctg atcattaccg ggccgaacat ggcaggaaag
     1021 agcacctatc ttcggcaggt cgccctgatc gtgctgatgg ctcaaatcgg cagcttcgtt
     1081 cccgcagcag aggcgcatat tggcctgacc gatcgcatct tcacgagagt gggagcctcg
     1141 gataatttgg cgggaggcca gagtacgttc atggtggaga tggtcgaggc ggccaatatt
     1201 ctccagaacg ccactcaacg cagcctgatt ctgttggacg aaatcggtcg cgggacaagc
     1261 acctacgatg gtctgagcat cgcctgggcg atcgcggaac atatccatga ccgcgcacga
     1321 ttaggcgcac gcacgttatt cgccacccat taccatgaga tgacccagct ggagcaacaa
     1381 cggaccggaa tcaagaatta tcgcgtggcc gttcaggaat gcggcgggaa cgtcgtgttt
     1441 cttagaaaaa tcgtggcggg caaggccgac cggagttatg gtatccacgt cgccaagctt
     1501 gcagggctcc ccaccactgt gatctgtcgg gcgcaagcgg tgttgtcgca acttgaacaa
     1561 ccggagtcgg ctcgttccgg gttgattcag gaacagcccc cccggacgtc tgagcccctt
     1621 ccccagcctc atccactcat tgaggaggtc aagcagatcg acttattctc aataactcca
     1681 cttgatgcac tgaatcgtct tgccgagttg caacgcatgg tgagatcgga cagcagggat
     1741 tcatccgatc gataactctc gtggatcgga tggtgaaaat gaaacgggcg gcatttccag
     1801 cgtgaaatgc cgcccgtttt catctgaata gccttcagtg cgctcgatta tactgtgcgg
     1861 accagggaat gagaccaccg agggtttttc caccaggaag catcacatca tattgcatgc
     1921 ccacattgtg cccatcagga gtgaccggcg aagtgttgag atcttgcttg cctgctgcgc
     1981 aagccttgtc gatttcactt ttcccggcac aatctttgag gcgaagcgcg ga
//
LOCUS       DATNDD010000031         3881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2856882, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000031 DATNDD010000000
VERSION     DATNDD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856882"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VN647_00725"
     CDS             complement(<1..277)
                     /locus_tag="VN647_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG95575.1"
                     /translation="MDSSVVRKRVLLIDDDPRVRASLKMVLEPLYDILQASDAHEGLD
                     VFRKDEPDLILLDVILPGTDGLAVLQTLRMESKTTPVIMLTGTKSVKT"
     gene            complement(293..1573)
                     /locus_tag="VN647_00730"
     CDS             complement(293..1573)
                     /locus_tag="VN647_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG95576.1"
                     /translation="MTDRTAPQSSPCQSGTISLRQLTQFARDMGHPTSLPVNAERILS
                     GLSEVSQHSQGVLYLLDRERECFCRVISQTQIPSIAAPPVVPLNYPLVQRLADHQDIL
                     DSSVLIDSLCRASLDNTAMDAQLATLPITLAIPLLNRGRLIAFVLLQSGIGTAVIPSH
                     TMELLAAMAQNAANALDSFLLYEDLRQSQALMRRTDRLRSLETIAGGFAHEIRNPLTS
                     IKTFIQLAPERKDDSQFIGEFSRIVLDDVNRIERLIQEILDYARYMEPRLTDEDLNEI
                     VSSCLYFIQVKADSRGIKIEKDLAPELPRVMLDRQQIKQVLLNLLLNAMDAMSEKIGT
                     IRVRTTKLQKADGEVWTQIEIEDTGHGISPENLDHIFDPFFTTKYASAVNEGAGLGLT
                     IAHQIIREHRGEIQVQSTEGIGTTFRINLPSHRG"
     gene            complement(1675..>3881)
                     /locus_tag="VN647_00735"
     CDS             complement(1675..>3881)
                     /locus_tag="VN647_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG95577.1"
                     /translation="ITVEAQFPPLHKTGDYVATHTENGLQGTDSRETVRVRVSLPVRI
                     ETSEAAAGSLLGIAINLSRGGVCVQTESFLKMTDEKIAIHFSSIGHHDQQRVQKPKAP
                     EAILTGRIVHLAPHPAVSSELTAGPSQPGQRIGIRFSQLTPFAEREVNRVLEQYIGSS
                     VDVEDRVGRPLIMSDRWECRNTHGQTIAVTADHARHEISLDTPIIVVIPGFGSTQTDY
                     VPLSFYLAANHLRVLRYDHSNHVGQSEGSILQITLRNMQTDLQSILDFVHTTWPAAPV
                     TLLAEDIAARVAVKVMARSTSADRLFLANPVLDLETALSLIDRSNVIETYRPGHRHGV
                     VNLWGLNVNLDKFIGDAITGEYVDLASSRADFAQLAAPPVILISPRKYRPIEHMFGPQ
                     YESLSTLTPRPTVVSLPTDVSGKSEANDERRTEAFKAILKLISTSLIGDSPSAQVREP
                     SFRDVDRQRQLEHERIRIRYHVSQATRSALWGAHLAHLPQLENLPDYLALTNELYRRL
                     LPLEPGMTILDMGYGQRNFVRLMLTNQAYRSAHQSGPTTIPLRYIGLDQSHESLRLAE
                     GQTHTFAEELPATLAAAVPVGQRLATSWTHTDWNVPFPFTDGSIARILCNLSLSFTPS
                     PIYCLRQMLRVLHPDGTAVVTCVQPHTDLSMLFRRHLRAADRDEFDSPAKVVLHYLGR
                     LREAVRHGLLHTYERDELAGLLAHAGAGSIQFFPVLDNQLLLAIVRKTKSAG"
BASE COUNT          885 a          950 c         1176 g          870 t
ORIGIN      
        1 ctgttttgac ggatttggtg cctgtaagca tgatgacggg ggtcgtcttg ctttccatgc
       61 gaagcgtttg aagcacggca aggccgtcgg ttcccgggag aatgacgtcg aggaggatga
      121 ggtcgggctc atcctttcga aacacatcga gcccctcatg cgcatcactc gcttggagaa
      181 tgtcatagag tggttcgaga accatcttga gagaggcacg gactcgggga tcatcatcga
      241 tcaacagaac tcgtttccta acgaccgagc tgtccacaag cgctccttgc gtctaacccc
      301 gatgggaggg aagattgatg cggaaggtcg ttccgatccc ttccgtgctc tgaacttgga
      361 tctctcctcg atgctctcga atgatctgat gagcgatggt cagtcccaac cccgctcctt
      421 cattgacggc actcgcatat ttcgtggtaa aaaatgggtc aaagatatgg tcaagatttt
      481 cgggtgagat gccgtgcccc gtgtcttcaa tctctatctg agtccacacc tcaccgtccg
      541 ctttttggag cttagtggtc cgtactcgga tggttccgat tttttcgctc atggcatcca
      601 tcgcattcaa gagcaggttt aaaaggacct gtttgatttg ttgccgatct aacatgacac
      661 gaggaagctc aggcgccaaa tctttttcaa tcttgatccc gcggctgtcg gctttcactt
      721 ggatgaagta caggcacgac gagacgattt cattcagatc ctcatcggtc aaccgcggtt
      781 ccatgtatcg agcgtagtcg aggatctcct gaatcagcct ttcaatccga ttaacgtcat
      841 cgagcacaat tcgactgaat tctccgataa attgactgtc atccttacgt tccggagcca
      901 gctgaatgaa ggtcttaatc gacgtcaagg ggtttcggat ctcatgagca aagcctcccg
      961 caatggtctc taaagaacgc aatcgatcgg ttcgtcgcat gagggcttgg gactgccgga
     1021 gatcctcata cagcaggaaa gagtcgagcg cgttggcggc attctgggcc atcgcggcca
     1081 ggagttccat ggtatgggag ggaatgacag cggtacctat cccggattga agcaatacga
     1141 aggcgatcaa ccttccccga ttcagcagcg ggatggccag agtgatggga agagtggcga
     1201 gctgggcatc catcgcagta ttgtccaacg acgctctaca tagggaatcg attaacacgg
     1261 acgagtcgag aatgtcttgg tggtcagcca gtcgttgaac aagaggataa ttgagaggaa
     1321 cgacgggagg cgctgctatg gaagggatct gcgtttgtga aatcacccgg cagaaacatt
     1381 cgcgttcgcg atcaagcaga tatagaaccc cttgagagtg ctgggatact tcagagaggc
     1441 cggaaagaat gcgctctgcg ttgacgggga ggcttgtcgg gtgccccatg tcccgtgcga
     1501 actgcgtgag ttgccgcagc gatatcgtgc ctgattgaca aggggatgat tgcggagccg
     1561 tgcgatccgt catgagcgtg gtgtgcgagg tcccatgtgc ctgcgtagtc cgtcgtggta
     1621 aaagcgtggc tacgtatcat ccctaggtga aaagtataca cccgagagac tgtttcatcc
     1681 agcggatttg gtcttccgta caatggcgag gagaagttgg ttgtcgagga cggggaaaaa
     1741 ttgaattgac cccgcaccgg catgagccag cagaccggcg agctcatccc gttcataggt
     1801 atgcaataag ccatggcgaa cggcttcacg cagacgtccg agatagtgaa ggacgacctt
     1861 ggccggagaa tcgaattcat cacggtctgc tgcgcgcagg tggcggcgaa acagcatcga
     1921 gagatcggtg tgcggttgaa cgcaggttac gacggccgtt ccatccggat gcaagactcg
     1981 aagcatctgc ctgagacaat aaatgggaga gggggtaaag gagagcgaaa gattgcagag
     2041 aatgcgcgca atcgatccat cggtgaaagg gaaaggtaca ttccagtccg tgtgagtcca
     2101 gcttgtcgct aaccgttggc cgactgggac ggcagccgcg agtgtggcgg gaagttcttc
     2161 agcgaatgta tgggtttgtc cttctgcaag tctaagcgat tcgtgcgact ggtccaaccc
     2221 gatatagcgg agaggaatgg tcgtcggtcc gctttgatgt gccgaccgat aggcctgatt
     2281 ggtcaacata aggcggacga agttcctttg gccgtagccc atgtcgagga tagtcatgcc
     2341 tggttcaagg ggaaggagcc ggcgatacaa ttcattcgtc aaggccaaat agtccggcaa
     2401 gttttcgagc tggggcagat gagccaagtg agcaccccac aaggcgctgc gtgtggcctg
     2461 cgagacgtga tagcgaatac ggatgcgttc gtgctccagc tgccgttgcc gatccacatc
     2521 gcggaagctc ggttcccgga cctgagccga gggagaatcg ccgatcagag aagttgagat
     2581 caacttcaag atcgccttga aggcttcggt acggcgctcg tcgttggcct cggattttcc
     2641 agacacgtcg gtcggcagcg atacaacggt tgggcgaggg gtcagggtgg agagggactc
     2701 atattgaggg ccgaacatgt gttcaatggg gcgatacttt ctgggagaga tcaggataac
     2761 aggcggagca gccagttgag cgaagtcagc tcttgacgag gccaagtcga catattctcc
     2821 cgtaatcgca tcaccgataa acttgtcgag gttgacattg aggccccaga gattgacaac
     2881 gccatgccga tggcctggcc ggtaggtttc aatgacattg gagcgatcaa taagtgaaag
     2941 agcggtttct aggtcaagga caggattcgc caagaaaagc cgatcagccg atgtgcttcg
     3001 ggccatgact ttcacagcca ctcgtgcagc aatgtcttcg gcgaggaggg tcaccggagc
     3061 ggcgggccat gtggtgtgga caaagtccag aatgctctga agatcagtct gcatattgcg
     3121 caatgtgatc tgcaagatgc tgccttcgct ttggccgacg tgattcgagt ggtcgtagcg
     3181 caacacgcgc aaatggttgg cggcaaggta aaacgagagt ggtacgtagt cggtttgggt
     3241 cgatccgaat cccgggatga cgacgatgat cggggtgtct agggaaattt catggcgagc
     3301 atgatcggcc gtcaccgcaa tggtctggcc atgcgtattg cggcattccc atcgatcgct
     3361 catgatcaat ggccggccca ctcgatcctc aacgtctacg gaggagccga tgtattgttc
     3421 aagcacgcgg ttgacttccc gttcagcgaa tggcgtcagt tgggaaaaac ggatgcctat
     3481 gcgctgtcct ggttgtgatg ggcctgccgt gagctcggat gaaacagcgg gatggggagc
     3541 gagatggaca atgcggccgg tcaggatcgc ttcgggtgct ttcggcttct gcaccctctg
     3601 ctgatcatgg tggccgattg aagagaaatg aatcgcgatc ttctcatcgg tcattttcag
     3661 gaacgactcc gtttgcacgc ataccccgcc tcgactgagg ttgatggcaa tgcccaacag
     3721 acttccggct gcagcttcag aagtttcaat gcggaccggc agcgagactc gaactctcac
     3781 cgtctcacga gagtccgtgc cttgaaggcc gttttccgtg tgggtcgcta cataatcgcc
     3841 cgtcttatgg agcggcggaa actgagcttc gaccgttatg g
//
LOCUS       DATNDD010000032         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_539728, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000032 DATNDD010000000
VERSION     DATNDD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539728"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VN647_00740"
     CDS             complement(<1..672)
                     /locus_tag="VN647_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWG95578.1"
                     /translation="MAFQTVLNRYLPSLQDCRDFIVSDLNKFPDTTILSNYLQGGKMF
                     RALLAFMAADALGSELATMIPVASALELLHGASLIHDDIVDHAKERRGQRALHVQVGM
                     GPALILGDYLMLRSFTLLQQSQDNFGLQRVTDAVKTLTSYAQACCLGELRELTIESEG
                     DQEGEYLRIVEGKTASQFAASVTVPAILGGGTQDEIEALRTYGLNLGIAFQIHDDVLD
                     IDEDTV"
     gene            complement(758..1027)
                     /locus_tag="VN647_00745"
     CDS             complement(758..1027)
                     /locus_tag="VN647_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HWG95579.1"
                     /translation="MTLWPIFVTVFLAELGDKTQLATLLFAAEQNTSKVGVFLASAGA
                     LVLSSLIAVLMGAQLSAYVPLRALKIVAGIGFVAIGLWVLLDART"
     gene            complement(1470..2492)
                     /locus_tag="VN647_00750"
     CDS             complement(1470..2492)
                     /locus_tag="VN647_00750"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HWG95580.1"
                     /translation="MRYANPPNLLGGLVLFVVLASCGPTPQAGGGIGGTGATASIVAS
                     GPITGFGSIFVSGTEYDTTHTSMMVDGNSGNQSDLKKGMIVRVNATVPEGSGMDEPVL
                     RTANTVLYEDTVEGFVQSVAADGTGLIVLGQTVTITPATAIDVSILGGSVANLIPGRD
                     LVEISGFVMGDGLIRATWVELKRLDQKTDAPDYHAKGFIKHHHADQHTFEIGDLTLDY
                     RNAVLNDMPAQSNNGWDGLLIDVAGNQTSTEGSGSSRIRLTATRVSQENLGLGEGEVA
                     DIEGIVTQTLRAGDFFIGNVHVLTDAGTTFQGGALNEIRVGVHMEVHGSLVGGMVKAT
                     AVEFKP"
     gene            complement(2489..>3094)
                     /locus_tag="VN647_00755"
     CDS             complement(2489..>3094)
                     /locus_tag="VN647_00755"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HWG95581.1"
                     /translation="SMTSVSAEEYHRGSRVITGWLRDPKYGDGRGHPRPLAMDEGSAS
                     FSALVNSYSGDIPVRAVLDELLRVGAVRQLKDGRICLVSRGYIPQKGSVEKLQVLGAD
                     TADLISTLDYNIYVKPTKPRFQRKVMYDNVPREAAKEFRTVVAAEGQEFLEKLDRWLA
                     HHDRDVNPASKGTGRVRVGLGVFQFEEQEPPGDSHREDPSS"
BASE COUNT          704 a          895 c          819 g          676 t
ORIGIN      
        1 caccgtgtct tcatcgatgt ctagcacatc atcgtggatc tgaaacgcga tgcccagatt
       61 gaggccatag gtccgaaggg cctcaatctc gtcctgcgta cctccgccta aaatggctgg
      121 caccgtgacg gacgcggcga actgagacgc cgtcttccct tccacaatgc ggagatactc
      181 accctcctga tctccctcac tctcgatcgt gagttcacga agttcaccga ggcagcatgc
      241 ctgggcatat gaggtgaggg tctttacagc gtccgtcact cgttgcagac cgaagttgtc
      301 ttgtgactgt tgtaagaggg taaaggagcg aagcatcaga taatcaccta agattagagc
      361 cgggcccatc cccacctgga catggagcgc gcgctggccc cgccgttcct tggcatggtc
      421 cacaatatcg tcgtgaatga gcgacgctcc gtgcaggagt tcgagggctg aggcgacggg
      481 gatcatcgtg gccagctcac taccaagagc atcagccgcc ataaaagcga ggagagctcg
      541 gaacatcttg ccgccttgaa gatagttact cagaatggtg gtatctggga atttgttgag
      601 atccgagacg atgaagtccc gacagtcttg cagggatgga aggtaccgat tcagaaccgt
      661 ttgaaaggcc attgagttcc ctcacataag cttcccatcc agactgcgct gtgcggctca
      721 gcatacagcc gattgaggct taaccccagt ttgatcgtca tgttctcgca tcgagaagga
      781 cccaaaggcc aatggcgaca aacccaatgc cagctacaat tttgagtgca cgaagcggga
      841 cgtaggcgga gagctgagcg cccatcagga ccgcgatcaa gctggagagt acgagcgcgc
      901 cagcggacgc gagaaagacc cccaccttgc tcgtgttctg ctctgcggca aagagtagcg
      961 tggcaagctg cgtcttgtcg ccgagttcag cgagaaagac ggtcacaaaa atgggccaga
     1021 gcgtcatctc catgcctcct tcttacggac tgagaggaaa accgtcggcc gcacgtggga
     1081 gataggccaa acgtccttcc cataaagagg tgtgccatcg gcgatagcgt agcagtatgg
     1141 gaaaatattc ccaataagtc aaggaggttc tcaggcctcc gcacaaaacg gaaacaggca
     1201 ccctctcact cgaatctaat cgacatggtt tctgattgga gaacatgtgc aatccctgat
     1261 ggactcaatg atcactctac ttggctgggc tcccttcttt gcgagcgagc tggtagagag
     1321 cgcacagggc tgccgtgcga cctgaagatg gatactcaag ggtagcgctt tgccattagc
     1381 cattacgttg gctaacaaga cttcgtggag gctcatgcca ggcaggatgg cttaaatacg
     1441 tctgcagcgg cttcggcgag agccttcgtt tatggtttaa actccaccgc cgtcgccttc
     1501 accatgccac cgacgagtga accatgcacc tccatgtgaa cacccactcg aatctcgttc
     1561 agcgcgccgc cttggaaggt ggtcccggcg tctgtcagta catgcacgtt gccgataaag
     1621 aaatcccccg ccctcagtgt ctgggtgacg atgccttcga tgtccgccac ctcaccttcc
     1681 cctaacccta agttctcttg tgacacacgg gtcgcggtaa gacgaatcct ggaagagcct
     1741 gagccttcag tcgaagtctg atttcccgcc acatcgatca ataagccatc ccacccgtta
     1801 tttgactgag caggcatatc gttcaacact gcattgcggt agtcgagcgt gagatcccca
     1861 atttcgaagg tgtgttgatc cgcatggtga tgtttgatga accctttggc atgataatca
     1921 ggcgcatcgg ttttctggtc gagtcgtttt aattcgaccc acgttgccct aatcagcccg
     1981 tctcccatca cgaacccgct aatctccaca agatcgcggc ctggtatcag attcgcgaca
     2041 ctgcctccca ggatactgac atcgatggca gtcgcaggcg tgatggtgac agtctgaccc
     2101 aacacgatca gaccggttcc atccgccgca accgattgga cgaatccttc gacggtatcc
     2161 tcatacaaca ccgtattggc tgtgcggagt actggttcat ccatgccgga accttccggc
     2221 accgtggcgt tcacacgcac gatcattcct tttttgagat cgctttgatt ccccgaattc
     2281 ccgtcgacca tcatggacgt gtgcgtggtg tcatactcag tgccagagac gaaaatgctg
     2341 ccgaatccgg tgatgggtcc tgaggcgacg atggaggcgg tagcaccggt ccccccgatg
     2401 ccgccgcccg cttgcggagt cgggccgcac gaggccaaca ctacgaacag gaccagcccg
     2461 ccaagaagat tcggagggtt tgcatatctc atgatgaggg atcttctcga tgagaatcac
     2521 caggtggttc ttgctcttcg aactgaaaaa ccccgagccc cacacgtacg cgaccggttc
     2581 ccttcgacgc cggattgaca tcacgatcat gatgagcgag ccagcgatca agcttctcca
     2641 aaaattcctg tccttcagcg gccacgacag tgcgaaactc ttttgccgct tctcggggca
     2701 cgttatcgta catgactttg cgctggaagc gaggcttcgt aggtttcaca tagatattgt
     2761 agtccagtgt cgagataagg tcggcggtat cggctccgag cacctgtagt ttttccaccg
     2821 aacccttttg agggatatat ccccgcgaga ccagacaaat tctcccatcc ttcaattgtc
     2881 tcacggcacc gacccgtaag agctcatcca gcacagctcg caccgggata tccccgctgt
     2941 aggagttcac caacgcactg aaagatgcgc ttccttcgtc catggcaagt gggcgcggat
     3001 ggcccctccc atctccgtat tttggatcgc ggagccatcc ggtgatcacg cgtgaacccc
     3061 gatggtattc ttcagcggag acggacgtca taga
//
LOCUS       DATNDD010000033         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3126709, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000033 DATNDD010000000
VERSION     DATNDD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3126709"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1063)
                     /locus_tag="VN647_00760"
     CDS             complement(116..1063)
                     /locus_tag="VN647_00760"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HWG95582.1"
                     /translation="MQNQLDRRGRTLAAVMFLASAAGELTGCASQQTFASPEEGKVAL
                     VEAIRADDSARLQTILGRHGRELFRSGDTVADQHYREAFLKAYGEAHSLIIEGEERAV
                     LVIGRSAWPVPIPLLQSPDGWRFETAQGEQQILARRIGRNQWAAMQVCFAVVDAVNEY
                     ATQDMNDNGIPDYTPRFVSTPGTKDGLYWPAKAGEPPSPLSPLLAAAMSEGYSDATSK
                     PLAPYHGYFYRVLTKQGEAAPGGAYNYVIKGIMIGGFALIAYPAEYGVSGIMSFLINQ
                     DGVVYEQDLGKDTLAIVSEITAFNPDSQWRSVRVLAKDR"
     gene            complement(1454..1795)
                     /locus_tag="VN647_00765"
     CDS             complement(1454..1795)
                     /locus_tag="VN647_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95583.1"
                     /translation="MSTRQRFFRYGLTLALFGAALIPMTVLAEDRSFYSPVIYIDQEQ
                     NQILISTSGKVFYVEVPEAAKPHIEKLPISGLVDFVVEMRGEDQRPLIKTWKVKSGES
                     TCMYFNGKECQ"
     gene            complement(1871..>2577)
                     /locus_tag="VN647_00770"
     CDS             complement(1871..>2577)
                     /locus_tag="VN647_00770"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWG95584.1"
                     /translation="ENASQLRSRIAQALNVPQTVRLGPSLAAALPDSRFDHPAHTSTN
                     IPPIPSTTDSKSFRLLQDRLIVADLIIPRQILDDIIAHAKELTPYECCGLLAGTNGVV
                     SRLYRIKNIVAMEGAQNLSSFDSAKAAHLERLSPAERAEIAFVMDMQDFSASKKDMRN
                     NGLDLQVIYHSHPHDPARPSITDIKIATDYEEIWPKINLPLPAYLLVSLMNAEPDVKT
                     YWIKSGRVTPTDILIR"
BASE COUNT          571 a          664 c          743 g          599 t
ORIGIN      
        1 cggcacggcg gtacatccga cgctcacgca caggactgat aaacagagaa ttcgacccaa
       61 agtgatcaca ctcatcatct agatactcct acttccccgc tgattcccgc aggcgctatc
      121 gatccttggc aagaaccctc acgctcctcc actgactatc gggattgaag gcggttatct
      181 cggacacaat cgcgagagta tccttcccta gatcctgttc gtagaccacc ccgtcctgat
      241 tgatcagaaa actcatgatg ccggagacgc cgtactccgc cggatacgca atgagggcaa
      301 acccgccgat catgatgccc ttgatcacat agttgtaggc tccacccggt gccgcctcgc
      361 cctgctttgt gagaacccgg tagaaatatc catggtaggg ggccagcggt ttggaggtcg
      421 cgtcgctata gccttcgctc attgcggcag ccagcagcgg gcttagggga ctcggcggct
      481 cgcccgcctt tgccggccaa tagaggccat ctttggttcc gggcgtgctc acgaatcgtg
      541 gggtatagtc gggtatgccg ttgtcattca tatcctgcgt ggcgtattcg ttcacggcat
      601 ccacaacggc gaaacagact tgcatcgccg cccattggtt acgtccgatg cggcgggcca
      661 agatctgctg ttcgccttgg gctgtttcaa acctccagcc gtcgggggat tgcagaagtg
      721 gaatgggcac gggccaagcg ctacggccaa tcacgaggac tgcgcgctcc tccccttcga
      781 tgatcagtga gtgtgcctcg ccataggcct tgagaaacgc ttcgcgatag tgctgatcgg
      841 ccaccgtgtc gccggaacgg aacaattcac gaccgtgccg gccgagtatg gtctgtagcc
      901 tcgctgaatc gtctgctctg attgcttcaa ccaacgcgac cttgccttct tccggtgagg
      961 cgaaggtttg ttgggaagca cagcccgtaa gctctccagc cgccgatgcg aggaacatga
     1021 ctgcggcaag ggttctgccc cgtctatcca attggttctg catacccacc tcctaccgtg
     1081 tgtcggttca ttgttgtatt catgacgaca aagacaacag cacgatgagc atgcgagtta
     1141 ggatggatgt cataggacga catccatcgc tgaaccagaa cctggcacat gcattcgttc
     1201 gaaaggaatg gttacccatc ataccacccc ggtgtgtcat ggtggatggc cagatccgtt
     1261 gaatatggag gcgattttga cgagctgtgc ttggctgggg cagccgtttc cggagccgtg
     1321 gctactcaag attcgctgaa accttagatt gccgactgag gaactcggaa tagatagctc
     1381 gatatcagga gagcgggata cgggatgtct attgcctgat gccgtgtaaa agccggcgtg
     1441 ataagccctt cacttattga cactctttcc cattgaagta catgcaggtc gattcgcctg
     1501 actttacctt ccaggtttta atcagtggac gttgatcctc tccccgcatt tcaacgacga
     1561 aatccacaag gccggaaatg ggaagctttt caatatgtgg ttttgcggct tccggcactt
     1621 caacataaaa gaccttcccg ctcgtggaga tgagaatttg attctgctcc tgatcgatgt
     1681 aaatgaccgg actgtaaaaa ctgcgatcct cggcgaggac cgtcattggt attagagcgg
     1741 ccccgaacag tgcgagggtg aggccatagc gaaagaatct ttgtcgcgtg gacatgcaca
     1801 acgctcctta ttcaggctgc acgggaacat tattacattc cggcgaagat gtgaactgta
     1861 aaaatctgtc tcaacggatg agtatgtcgg tgggagtgac gcgaccggat ttgatccaat
     1921 aggtcttgac gtccggttct gcattcatga gcgagaccag gagataggcg ggaaggggca
     1981 agttgatctt gggccaaatc tcttcgtagt cggtggcaat ttttatgtcg gtgatcgacg
     2041 ggcgggccgg gtcgtggggg tgcgagtgat agatgacttg cagatcgagt ccattgttgc
     2101 gcatatcctt cttcgaggct gagaaatcct gcatgtccat gacaaaagca atttccgctc
     2161 gttcggcagg cgaaagccgc tcgagatgcg cggcttttgc ggaatcgaat gaagagaggt
     2221 tttgtgcccc ctccatcgca acgatattct taatgcgata gagacggctg accactccat
     2281 tagttccggc gagcaacccg caacattcgt aaggagtgag ttccttggca tgggcgatga
     2341 tgtcgtcgag aatctgtcgg gggatgatga ggtcggccac gatcagtcga tcctgaagta
     2401 gccgaaacga ttttgagtca gtggtggagg ggattggagg aatattggtt gatgtgtgtg
     2461 caggatggtc aaaacggctg tccggcaagg ccgcagcgag cgaagggccg aggcgtacag
     2521 tttgaggtac gttgagggcc tgagcgatgc gagaacgaag ctggctggcg ttttcaa
//
LOCUS       DATNDD010000034         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3124820, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000034 DATNDD010000000
VERSION     DATNDD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3124820"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VN647_00775"
     CDS             complement(<1..276)
                     /locus_tag="VN647_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95585.1"
                     /translation="MTRRNVGLSALMAGCALLLSGGMASAEDLPPLPPVPPEYADKKM
                     PAGGWTDAKAIEEGGKIYKGEFKTEVNCSSCHGTDGQPKKKGARDLRD"
     gene            complement(557..>2112)
                     /locus_tag="VN647_00780"
     CDS             complement(557..>2112)
                     /locus_tag="VN647_00780"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWG95586.1"
                     /translation="EGHPTPRLPFVDMATGSLGQGLPVGIGIALNAKFVDRLDYRTYV
                     LMGDGESVEGSVWEAAEIGRQYGLDNLCAIVDVNRLGQSDPTMLQHDMDAYRSRWAGF
                     GWHAIVVDGHDFTAILKAFDEAARTKGTPTVLLARTYKGKGISFIENKDGWHGKPLKK
                     GEETQKALDELTKQLSPNGVVIQIPKPSAPDTAPTAIRPMPPSPYKIGDSVATREAFG
                     VALETLGSVNPSIVALDADVKNSTYTDKFGKKFSNRFFESFIAEQNMIGAAAGLAACG
                     KIPFAATFACFFSRAYDFIRMAAISGSNIKLVGTHVGVSIGEDGPSQMGLEDIAMMAA
                     QPNVTVLYPSDGNSTYQLIEEAAKHKGMVYIRAGRPKTPVLYGPEERFRIGGSKILRQ
                     SAADVLTIAAAGVTLFEALKAYDQLKSAGIAVRVIDLYSIVPIDRNSLLESGRATQRR
                     ILTVEDHYAHGGLGDAVLSAVATEGMKVFKLAVREIPHSGKPEELVDHYGIGVRSIVE
                     ATKRIVREP"
BASE COUNT          436 a          584 c          561 g          531 t
ORIGIN      
        1 gtcacgaaga tcccgcgcgc cttttttctt tggttgaccg tctgttccgt ggcaactgct
       61 gcagttgacc tcggtcttga actcgccctt ataaatcttc ccgccttctt caatggcttt
      121 cgcatcggtc catccaccgg caggcatttt tttatcggca tattccggtg gaactggagg
      181 caacgggggc agatcttcgg cggaggccat gcctcctgaa agcagaagag cacatcccgc
      241 catcagcgct gagaggccca cattccttct cgtcattcgc acctcctctt gaattgtaat
      301 gaatgaatta cccgtcttgt ttgcttgagt cggttgctta gtcacagtgt gcagcgcact
      361 cttgatcggt acgcactata ccatattttc catcggaaaa aatgaaaaca tgagaagcct
      421 cgtgcggtca tgtgacccga cttcgtggca tcgattcatg accgcaggga atgtttcgta
      481 aggtgatcct gtttgggctc atcagatgat cggcggtttc tcaggcggac ggtttttgtt
      541 cgggccgttc catgattcat ggttctcgga cgattcgttt ggttgcctca acgatggagc
      601 gcactccaat cccatagtga tcgacgagtt cttcaggttt tccactatgc gggatttcac
      661 gaacggcgag tttaaagact ttcatgcctt ccgttgcgac agcgctaaga acggcatcgc
      721 ccagacctcc atgggcgtag tgatcctcaa ccgtgaggat tcgacgctgc gttgcacgac
      781 cgctctccag caagctattt ctatcgatgg ggacgatgct gtaaagatcg attacacgga
      841 cggcaatgcc cgctgacttc agctggtcat aggctttcaa ggcttcaaac agcgtcactc
      901 ccgccgccgc aattgtgagc acatccgctg cgctctgccg gaggattttg ctgccgccga
      961 tgcggaaccg ttcctctggt ccgtagagga ccggagtctt tggccttcca gcgcggatat
     1021 agaccattcc cttatgtttc gcagcttcct cgattaattg atacgttgag ttgccgtctg
     1081 aaggatagag caccgtcacg tttggttgtg ccgccatcat tgcgatatct tccaagccca
     1141 tttgcgacgg cccatcctcg ccgatgctta cgccgacatg agttccgacg agcttaatat
     1201 tcgagccact gatcgcggcc atacggatga agtcgtaggc gcgactgaag aaacaggcaa
     1261 aggtcgctgc aaacgggatt ttgccgcagg cagccagacc ggcggcggca ccgatcatat
     1321 tttgttcggc gatgaaactc tcaaagaatc gattggaaaa cttcttgccg aatttgtcgg
     1381 tgtacgtgga attttttacg tcggcgtcta atgcgacaat cgaggggttc acggacccca
     1441 aggtttccaa tgccacaccg aaggcttcac gagtggcgac ggagtcaccg attttgtaag
     1501 gtgagggagg catcgggcgg atggctgtgg gagcggtatc gggagccgat ggctttggga
     1561 tctgaatcac gacgccgttt gggctcaatt gcttcgtgag ctcgtcgagg gccttctgag
     1621 tctcctcacc tttcttaagc ggtttgccgt gccagccgtc tttgttctcg atgaacgaaa
     1681 tgcctttgcc tttgtacgtt cgggccagca acacggtcgg cgttcctttt gtccgggcag
     1741 cctcatcgaa tgccttgagg atggctgtga aatcatgacc gtccacgacg attgcatgcc
     1801 aaccgaatcc ggcccatcgg gaacggtaag cgtccatgtc gtgctgcagc atggtgggat
     1861 cactctgccc cagtctgttc acatcaacaa tggcgcacaa attatccaag ccgtattggc
     1921 gaccgatctc agcggcttcc caaaccgatc cttcgactga ttctccatcc cccatcaaca
     1981 cgtaggttcg gtaatcgagc ctgtcgacga acttcgcatt caacgcgatg ccgatcccaa
     2041 cgggaagtcc ttgccccagt gaaccggtgg ccatatcgac aaagggcaag cgaggagtcg
     2101 gatgtccttc ca
//
LOCUS       DATNDD010000035         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1965373, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000035 DATNDD010000000
VERSION     DATNDD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965373"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VN647_00785"
     CDS             <1..266
                     /locus_tag="VN647_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWG95587.1"
                     /translation="ARAEHAGKALIKGGVGGDRVRTVGYADSRPIATNDTEEGRSKNR
                     RVEIVVTQGSDPFASGGKDEQGASNTRSASASYGRMVQKVANH"
     gene            complement(461..1066)
                     /locus_tag="VN647_00790"
     CDS             complement(461..1066)
                     /locus_tag="VN647_00790"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWG95588.1"
                     /translation="MAQLVLLRHGESQWNLENRFTGWVDVPLSAKGVEEAKQAGEKLR
                     GFAFDRAFSSVLTRANETLRIVLEVLGQTHIPIEKDKALNERMYGDLQGLNKAETAKK
                     YGDAQVKIWRRSYDVKPPGGESLKDTAERALPYYQNMIKPHLLKGETIIIAAHGNSLR
                     ALVMELDQLSKEEVLELNIPTGVPLLYEFDDSGRVSSHRYL"
     gene            complement(1138..1791)
                     /locus_tag="VN647_00795"
     CDS             complement(1138..1791)
                     /locus_tag="VN647_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWG95589.1"
                     /translation="MTICRRCNQQNPDEAQFCHQCGAKLADATVAAESASETPTVRPI
                     QDDEMLWRQFIGPNADHYLSVFKKFSMNGQPKFALSWNWPAFLYISFLWFLYRKMYLH
                     AFVYAVGPMISTYLTGDFSAGIVWSIMAGATANYLYYWHCREHIGEIKKAGRMELAAQ
                     ETTLKESGGVQPYVTWVGVFFYIIFLATLMKMIQEGPPDLDHSPGRPAKQAVRLSQA"
     gene            1960..>2713
                     /locus_tag="VN647_00800"
     CDS             1960..>2713
                     /locus_tag="VN647_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWG95590.1"
                     /translation="MPSQNLRDDADKYLMQTYSRQPITIVRGRGTKVYDLEGRKYIDF
                     VGGIAVNVLGHGHPDLVQAIQRQAVQLIHTSNLYYTEPQVKLAKMLVDYSFADRVFFC
                     NSGAEANEAAIKLARRYGHERHGPDRFEVITMKNSFHGRTLATLTATGQEKVQKGFEP
                     LMPGFAYAPFNDFGAIESMVSEKTAAIMLEPIQGEGGVYEADRDYLRNLRDLCTRQDI
                     LLIFDEIQTGMGRTGTLFAYEQFGVKPDIMTLA"
BASE COUNT          635 a          718 c          737 g          623 t
ORIGIN      
        1 gggcgcgggc cgaacatgcc ggcaaggctc tgatcaaggg tggcgtaggt ggcgatcgag
       61 tcagaacagt cggatatgcg gactcacgac cgatcgcgac caacgataca gaagaaggcc
      121 ggagtaagaa ccgacgggtc gagattgtcg tgacccaggg atccgacccg ttcgcgtctg
      181 gtggaaagga cgagcaaggc gcgagcaaca ctcggagcgc ctccgcatca tacggaagaa
      241 tggttcaaaa agtcgcgaac cactgacgct gaactatgac cctgtcggcc gatgatcggc
      301 cgacagggtc atactgatct cctaatcctg catgcctccc ggtttcttgt cttaatacgg
      361 gtattgtagc gccaaaagat tgcgaaaagt tgttcgtctt atggatcgac catggaccga
      421 cggctgaata cgcaggttgc tcgacgaggc gggataagct ctacaaatac ctgtgtgacg
      481 ataccctgcc actgtcgtca aattcataga gcagcggcac gccggttgga atgttgagct
      541 ccagaacttc ttcctttgac aattgatcca gctccatcac caacgctcga agactgttgc
      601 catgcgccgc aatgatgatg gtttccccct tcagcaggtg cggtttgatc atgttctgat
      661 aatagggcaa cgcgcgctcg gccgtatctt tgaggctctc tccgccgggc ggtttcacat
      721 cataacttcg ccgccagatt ttcacttgcg cgtcaccata cttcttggcg gtttcagcct
      781 tattcagtcc ttggaggtct ccatacattc gttcgttcag cgccttgtct ttttcaatgg
      841 ggatatgcgt ttgcccgagg acttccagca cgattctcaa cgtttcattg gcgcgggtca
      901 ggaccgagga gaaggctcgg tcaaaggcaa acccacgaag cttctcaccg gcttgcttgg
      961 cttcctcgac accttttgcg gaaagcggaa cgtccaccca gccggtaaaa cggttttcca
     1021 agttccattg cgattcccca tgacgaagta agactaattg agccatcgca catcctttgc
     1081 cattaccggt gattgctcct tagccagaga caatgaatac tgaatgacta tggcaagtca
     1141 agcctgtgac aatcggactg cttgcttcgc cggtcgaccg ggcgagtgat ccaggtcggg
     1201 cgggccttct tgaatcattt tcatcagtgt cgccaggaag atgatataga aaaatacacc
     1261 gacccaggtc acataaggct ggactccgcc cgattctttc agtgtggttt cttgcgcggc
     1321 aagctccatt cgacctgctt tcttgatttc accgatgtgc tctcggcaat gccaataata
     1381 cagatagttt gcggttgctc ccgccatgat gctccagacg atgccggcag aaaaatcccc
     1441 cgtgagatac gtcgaaatca tcgggccgac cgcatagacg aatgcgtgca gatacatctt
     1501 acggtacaga aaccagagga aggagatgta gagaaacgcc ggccagttcc acgagagagc
     1561 gaacttcggc tggccgttca tcgaaaattt cttaaacacg ctcaggtagt ggtccgcgtt
     1621 ggggccgatg aattggcgcc atagcatctc atcgtcctga atcggccgaa cggtgggagt
     1681 ctcagaggca ctttcggcgg caaccgttgc atcagccagt ttggctccac actgatgaca
     1741 gaactgggcc tcgtcgggat tctgttgatt gcaccgtctg catattgtca tggcgacgca
     1801 gcttaccaca ctcggaacag aaggatacgg cctatcgcgt atggcggatt gcagaactgt
     1861 gaataagcga ctcctgacgg aatgttccga tgttccgctt attgctcttc caaccgcccc
     1921 tgtgctagct tttctttttt tctaatggga tacgcggtta tgccgagcca aaacctgaga
     1981 gacgatgccg acaaatatct catgcagacc tatagtcgcc agccgatcac gatcgtgcgg
     2041 gggcgaggca cgaaagtcta cgatctggaa ggccggaagt acatcgattt tgtcggtggg
     2101 atagccgtca atgtgctggg gcatggtcat cctgacctgg ttcaggccat ccagcgccaa
     2161 gccgtacaac tgatccatac ctcgaatctc tactacactg aacctcaagt gaaactggcg
     2221 aaaatgttgg tggactattc gtttgcagac cgtgtgttct tctgcaacag cggagccgaa
     2281 gctaatgagg cggcgattaa gcttgcgcgg cggtacggac atgagcggca tggacccgac
     2341 cgctttgaag tcatcacgat gaagaattcg ttccatggcc gaacgctggc aacgctcacc
     2401 gctacggggc aggagaaggt tcagaagggt ttcgagccgc tgatgccagg atttgcttat
     2461 gcaccgttca atgatttcgg cgcgatcgag tccatggtta gcgagaagac cgcggcgatc
     2521 atgctggaac caattcaggg ggaaggcggc gtgtatgagg ccgaccggga ttatctcaga
     2581 aacctgcgtg acctctgtac acgacaagac atcttgctca tttttgatga aattcaaacg
     2641 ggcatgggga gaacgggcac cttattcgcc tatgagcaat tcggcgtaaa acccgatatc
     2701 atgacccttg cta
//
LOCUS       DATNDD010000036         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3449986, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000036 DATNDD010000000
VERSION     DATNDD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449986"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VN647_00805"
     CDS             <1..772
                     /locus_tag="VN647_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG95591.1"
                     /translation="YWQEINALTMRWIRRLSRERFSMLFTLVQPMLFWLIFFGNLFQR
                     AADTQVMQAPNYISFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHRTSV
                     ILSRFIFVMAITSMQVLVILGVSYLFGVHPATGILGVATILLIGMMFGIGLTAISMAM
                     AFSVKSHGDFFSVLGFLSLPMIFLSSALVPLTAMPGWMSFLAQFNPMTWAIDAVRPLI
                     LSGWTEALPHVGMVVLVMLVFDALCLYGGAKAFRRAMG"
     gene            884..1741
                     /locus_tag="VN647_00810"
     CDS             884..1741
                     /locus_tag="VN647_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWG95592.1"
                     /translation="MIPENQIRALIRLLSDEDDRIVRTISGRLIDIGPSAVPLLQEAE
                     IEQPEMADRIASVLEEIRGSKLEDELANLAALSDEAMSLEAGAFLIARYAYPTLDLAR
                     YHEQLDTMADEVRARIGHRASGEEAVNSLNRYLFTEQGFKGNTKNYYEVENSYLNCVM
                     DRRVGIPISLSAVYLLIGQRLDLPLFGIGMPGHFLVKYESDRYKVFVDCFNGGALLTE
                     KNCARFLTEAGYGFDDKYLQKSPVRAILSRMIKNLLAIYSKSGDGGKTARMTTFIEIL
                     GGAPREEGL"
     gene            complement(1742..2497)
                     /locus_tag="VN647_00815"
     CDS             complement(1742..2497)
                     /locus_tag="VN647_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWG95593.1"
                     /translation="MRLTILGSGTNVHPTRAAAGYLVRTDHNILLDFGPRTLMNLIKS
                     GVNRHRITHILFSHFHADHFSDFITFFFDAVIYTKYGGGRRPGMTLIGPKGTIRLLQS
                     IMRSFPSFSPAPFRVVFKEVSSKPFSIGDTRIIPKPVVHVPDLSCVGYRIEYRGKAMV
                     YSGDSQYCDALARLCTDADLAVLDCSFPANRPGLAHLHAGQCGRIAREAGLGQLVLSH
                     FYPIADRYDVKAQAGDQYGGKIWKGKDLLTIRI"
BASE COUNT          557 a          695 c          728 g          568 t
ORIGIN      
        1 ctattggcaa gagatcaacg cattgacgat gcggtggatt cggcggttga gtcgggagag
       61 attcagcatg ctgttcacgt tggtgcaacc gatgctgttc tggctcatct tcttcggcaa
      121 cctgtttcaa cgcgccgcgg acacacaggt catgcaggct cccaactaca tcagctttct
      181 cgcggccggt gtcgtcgtca tgacagtcct gaacaatggc cttgccgggg gcgtcgatct
      241 gctcttcgat aaggaaaacg ggttccttga gcgtttgatg tccacgccga ttcatcgtac
      301 ctcggtcatc ctgagccgct ttatcttcgt catggcgatt acgtcgatgc aggtcctcgt
      361 gattctcggc gtgtcatacc tgttcggcgt tcatcccgcg acgggtattc tcggtgtggc
      421 gacgatcttg ctgatcggga tgatgttcgg gatcggcctg acggccatct ccatggcaat
      481 ggccttctcc gtgaaaagtc acggggactt cttctcggtg ttgggattcc tttcgctgcc
      541 gatgatcttc ctcagttccg cattggtgcc gttgacggcg atgccgggct ggatgagttt
      601 cctggcgcaa ttcaacccga tgacctgggc gatcgatgcg gtgcggcctt taattctgtc
      661 cggctggact gaggccttgc cgcacgtagg aatggtggtc ttggtcatgc tcgtgttcga
      721 tgccctgtgt ctgtacggcg gggccaaggc cttccgccgt gcaatggggt aacctcatca
      781 tgaacccaca acccatggat cgcttgactg acggcggaat ccgtcggatc atgatcttaa
      841 tgccgccata cgtcggtctg tgaacgacgg aaagtcgtag gagatgattc ctgaaaatca
      901 aattcgtgcg cttattcgcc tcctgtccga tgaggacgac cgaattgtcc gtaccatcag
      961 cgggaggctc atcgatatcg gtccatcggc tgtcccgctt ctccaagaag cggaaattga
     1021 gcagccggag atggctgatc gcatcgcttc cgtgctcgag gaaatccgag gaagcaagct
     1081 ggaggatgaa ctggcaaacc tggcggccct ctcggacgaa gccatgagct tggaagccgg
     1141 cgccttcctg atcgctcgct atgcctatcc gacgctcgat ctcgctcgct atcatgaaca
     1201 gctcgatacg atggccgacg aagtgcgagc acgtatcggc catcgagcct ccggagaaga
     1261 agccgtcaac tcactcaacc gatacctatt tactgaacaa gggtttaagg ggaacaccaa
     1321 aaactattat gaggttgaga acagctacct caattgcgtc atggatcggc gagtcggtat
     1381 ccccatcagc ctgtcggcag tttatcttct gatcggacag cggttggatc tcccgctgtt
     1441 cgggatcggc atgcccgggc atttcttggt gaagtacgag tccgaccggt acaaggtctt
     1501 cgtcgactgt ttcaacggcg gagccttgct gaccgaaaaa aactgcgccc gttttctgac
     1561 cgaggcagga tacgggttcg acgataaata cctgcagaaa agcccggttc gagcgatcct
     1621 gtctcgcatg atcaagaacc tcttggccat ctactccaaa tcgggtgacg gagggaaaac
     1681 cgcccgcatg accacgttta tcgagatcct cggcggcgcc ccgcgcgaag aagggctcta
     1741 gctagatccg aatcgtcagg agatccttcc ccttccaaat ctttccccca tactgatccc
     1801 ccgcttgagc tttcacatcg taccgatccg caatcggata gaaatgcgac agcacgagtt
     1861 ggcctagacc ggcttcccta gcgatccggc cgcactgtcc ggcatgcaga tgagcgaggc
     1921 cgggacgatt ggccggaaag gagcaatcga gtacggcaag atcggcatca gtgcaaagcc
     1981 gggcgagtgc atcgcaatac tgcgagtctc ctgagtacac catggctttc ccccgatact
     2041 cgattcgata gccgacgcac gagagatcgg gaacgtggac cacgggtttg gggatgatcc
     2101 gagtgtcccc gattgagaaa ggtttactcg acacttcctt gaagaccaca cggaacggcg
     2161 ccggcgaaaa acttgggaaa ctgcgcatga tcgactgtaa gagacggatc gtgcctttcg
     2221 gaccgatcag ggtcatccct gggcgacggc cccctccgta cttggtatag atcacggcgt
     2281 cgaaaaaaaa cgtgatgaag tcggaaaagt ggtccgcgtg aaaatgggag aacaagatgt
     2341 gcgtgatacg atgccgattc acgccggact taatgaggtt catcaaggta cgtgggccga
     2401 agtccaggag gatgttatga tccgtacgca ctaagtatcc cgctgcagcg cgggtcggat
     2461 gcacgttggt accggaaccg agaatggtca gacgcatagg cgaatcagtt atacagccat
     2521 atggttaaag aacgacacta agctgatc
//
LOCUS       DATNDD010000037         4386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1397723, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000037 DATNDD010000000
VERSION     DATNDD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397723"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VN647_00820"
     CDS             <1..212
                     /locus_tag="VN647_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95594.1"
                     /translation="AVVVNDEQAQRVLHGSPVFPMGAGQRSAAHAAVSVRLKNEAGQL
                     LAIGTRDSAHVGSIRIRKVLSLLSH"
     gene            230..499
                     /gene="rpsO"
                     /locus_tag="VN647_00825"
     CDS             230..499
                     /gene="rpsO"
                     /locus_tag="VN647_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWG95595.1"
                     /translation="MGLLKEAKGELIKQYQQHDKDSGSPEVQIAVLTTRITYLTEHFK
                     SHKKDHHSRRGLLQLVGRRRRLLDYLRGVDEARYRAVIDRLGIRK"
     gene            679..2796
                     /gene="pnp"
                     /locus_tag="VN647_00830"
     CDS             679..2796
                     /gene="pnp"
                     /locus_tag="VN647_00830"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWG95596.1"
                     /translation="MVHVVEVDIAGRTLRLETGRVAKQADGSIWASYGDTVVLATAVA
                     AQTAKPGTDFLPLTVDYQEKAYAAGKIPGGYFKREGRPAEKEVLTSRLIDRPIRPLFP
                     EGYYYETQVIASVLSADKTGSSDVIGITAASAALAVSNIPFDGPVAGVKIGRVNGQLV
                     VNPDLETMEQSELHLVVAGTADAVMMVEAGANELSEQTMLEALELAHAEIKKIVQKIE
                     ELAKKVGRVKREVLAESIDSALQAEIKTLVAQPIRNAIMIANKTARQERLDQILADAI
                     EKLKKPEDPSSERHVKIVFHQLEYTEVRKMILENRSRADGRGPTDIRPITCEVGALPR
                     AHGSAIFTRGETQSLAVVTLGTTDDEQRIDALEGEYTRTFMLHYNFPPFSVGEARPLR
                     SPGRREVGHGALAERALKPVIPNKDVFPYTLRIVSEILESNGSSSMATVCGGTLAMMD
                     AGVPIKEPVAGIAMGLIKEGDDVIILSDILGLEDHLGDMDFKVCGTKNGITALQMDIK
                     IGGITTALMQQALEQARLGRLHILDRMSKGLVGPRTDLSPFAPRIYTMKVKQDKIRDI
                     IGQGGKTIRGIQSDCGVKISVEDTGIVTIASADGASLQKAKEIINRLTEEVEVGKIYM
                     GTVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKAVSDEVAEGDQILVKVLEIDKQGK
                     IRLSRKETIPAPARNGANDSAGG"
     gene            2800..4065
                     /locus_tag="VN647_00835"
     CDS             2800..4065
                     /locus_tag="VN647_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWG95597.1"
                     /translation="MYRKLVLDNGIRLVTEQISTLKSVTVGIWVNAGSRDESPAEAGY
                     SHFVEHMFFKGTGTRSATDISREIDALGGEMNAFTTRETTTFYVKVLDQHLPKALDLL
                     SDLFLRSRLGRKEIEKEKQVVLEEIRMVQDDPEDLVQELHAKVVMGRHPLSRPILGRE
                     STIIRISRQNLLEYIDSHYRPEEIVVAIAGNFDHRQLEKTMTRTFGGYRTSSSGIPRK
                     RWPPELLGGAMMKQKSLEQVHLCVGFESVAAGHKDRYAVYALNAVLGGSVSSRLFQEI
                     REKRGLSYSIYSFLSGYSDSGTITVYAGIRAREVERVLDLVCREIRKLARHGIDGHEL
                     KRTKDQMKGSLMLSLESSHSRMNKLAKDELIAGTHTTLEDLIAEIDGVTEQQVFQTAQ
                     NLFIGDKIALTGLGPLSSRQVEGLSAKFS"
BASE COUNT         1052 a         1097 c         1285 g          952 t
ORIGIN      
        1 cggcggtggt tgtgaatgat gagcaggcgc agcgcgtcct gcatggctcg cccgtttttc
       61 caatgggagc cggccaacgg tccgctgcgc atgcggctgt ttccgtgcgt ctgaagaatg
      121 aagcaggcca gttgttggcc attggaactc gcgattccgc ccatgtggga tcgattagaa
      181 ttcgaaaggt cctgagtctc ttgagtcatt aatgcggaag gagttaggta tgggattact
      241 gaaagaagca aaaggtgaac tcatcaagca atatcagcag catgacaaag attccggatc
      301 gccggaggtt cagattgctg tgttgaccac ccggattaca tatcttaccg aacatttcaa
      361 gagccataaa aaggatcacc actcgcggcg tgggttgttg caactggtcg gacgcagacg
      421 acgtttgctg gactatctcc gtggtgtgga tgaggcgcgt taccgagccg tgatcgatcg
      481 actcggcatt cgcaagtaac ggtcagtaca acagcggcgt tgaccggcgc catatatccc
      541 acagatgaac accgacatgc gctcacgcga agtgttatca gggtgactga gcagctcaga
      601 cattcagttg ttaactcgtc acttgtcgtc gagcgcatct cagtgggcat ctgtggatct
      661 aaccagagga gaccatagat ggtacacgtc gtagaagtcg acattgcagg tcggaccctc
      721 cgcctcgaaa ccggtcgcgt cgcaaagcaa gctgatggat caatttgggc ttcgtatggc
      781 gacacagtcg ttctggcgac ggccgtggct gcgcaaacgg ccaaacccgg caccgatttc
      841 ctcccattga ccgtcgacta ccaagaaaag gcctatgcgg ccggaaaaat ccccggaggt
      901 tatttcaagc gagaaggtcg accggctgaa aaggaagtcc tgacaagccg cttgattgac
      961 cgtccgatcc gcccattgtt tcccgaaggg tactattacg agacacaggt cattgcctcg
     1021 gttctgtcgg cagataagac cggttcttcc gacgtcatcg gaattactgc ggcatcagcc
     1081 gccctcgctg tgtcgaacat ccctttcgac ggtcctgttg ccggggtgaa gatcggtcgc
     1141 gtcaacggcc aactcgtcgt caatcctgac ctcgaaacga tggagcagag cgagctgcat
     1201 ctggtggttg caggtacagc ggacgcggtg atgatggtag aggccggcgc caatgaatta
     1261 tccgagcaga cgatgctcga ggcgctggaa ttggctcacg ctgagattaa gaaaatcgtc
     1321 cagaagatcg aggaattggc caagaaggtc ggtcgggtga agcgggaggt tcttgcggag
     1381 tcgatcgact ccgcattgca agccgagatc aagacgttgg ttgcacaacc gattcgtaac
     1441 gccatcatga tcgccaacaa gaccgccaga caggaacggt tggatcaaat tcttgccgat
     1501 gctattgaaa aactgaagaa gcctgaggat ccctcaagcg aacggcacgt caagattgtg
     1561 ttccatcaat tggaatacac ggaagtccgg aagatgatcc tagagaaccg atcgcgtgcc
     1621 gacggacgag ggccgacgga tatcaggccg atcacctgcg aggtcggtgc cttgcctcgc
     1681 gcacatggtt ccgcaatctt tacccgaggc gaaactcaga gcttggcggt ggtgacgctg
     1741 gggacgaccg acgatgagca gcgcattgac gctttggaag gcgagtatac ccggacattt
     1801 atgctgcatt acaacttccc gcccttcagc gtcggcgagg cgcggccgct ccgctcaccg
     1861 ggaaggcgtg aggttgggca cggagccttg gccgaacggg cattgaagcc tgtcattcca
     1921 aacaaagatg tcttcccata taccttgcgg attgtttctg aaattctgga gtccaatggg
     1981 tcttcttcga tggcaaccgt atgcggggga acgctggcca tgatggacgc cggagtccct
     2041 attaaagagc cggtcgcggg catcgcaatg ggattgatca aagaagggga cgacgtcatt
     2101 atcttgtcgg acatccttgg ccttgaagat catctgggcg atatggactt caaggtctgc
     2161 gggaccaaga acgggattac ggcgcttcaa atggacatca agatcggagg cattaccacg
     2221 gcgttgatgc agcaagcctt ggagcaggcg cggttgggac gcctccacat tctcgaccgc
     2281 atgtccaagg ggcttgtggg cccgcgcacc gacttgtcgc cgttcgcgcc ccgcatctac
     2341 acgatgaagg tcaagcagga taagattcga gatatcatcg gtcagggcgg caagaccatc
     2401 cgcgggattc agtccgactg cggagtgaag atcagtgtcg aagataccgg tattgtgacc
     2461 atcgcttctg cggatggcgc gtcgttacaa aaagccaagg agatcatcaa ccgcttgact
     2521 gaggaagtcg aagtcgggaa gatctacatg ggtacggtaa gaaagattat ggactttggt
     2581 gcgttcgtgg aggtgttgcc gggcacggat gggttggtgc acatctcgca gcttgcgcat
     2641 caccgagtga aagccgtatc cgatgaggtg gccgaaggcg accaaatcct ggtgaaagtg
     2701 ctggagatcg acaaacaagg caagatcagg ctcagtcgaa aagagaccat tcctgcaccg
     2761 gcaagaaacg gtgccaacga ttcagcgggc gggtaacact tgtaccgcaa gctcgttctc
     2821 gataacggaa ttcggttggt tactgagcag atttcaaccc ttaagtctgt gacggttggt
     2881 atctgggtca atgcgggctc tcgtgatgaa agtcccgcag aggccgggta ttcgcacttc
     2941 gttgaacaca tgttcttcaa agggacgggt actcgttcgg cgacggacat ctcccgtgaa
     3001 atcgacgcgt tggggggcga gatgaacgcc ttcacgacac gcgaaacgac gacgttctac
     3061 gtcaaggtcc tggatcagca cttgcccaag gcgttggacc tgctttcgga tttgtttctt
     3121 cgctctcgtc ttggccggaa agaaattgag aaagaaaaac aggtggtcct cgaagaaatc
     3181 cgcatggttc aagatgaccc tgaagatctg gtccaggaac ttcatgccaa ggtggtcatg
     3241 gggcgccatc ccttgagtcg accgatcttg gggcgagaat caaccatcat ccggatcagc
     3301 cggcagaatc ttcttgagta tatcgactcc cattaccgcc cggaagaaat cgtggtggcg
     3361 atcgcgggaa atttcgatca ccgtcagctt gaaaaaacaa tgactcgcac cttcggcggg
     3421 tatcgtacgt cctcaagcgg cattcctcgc aaacgttggc ctcctgaatt gctggggggc
     3481 gcgatgatga aacagaagtc actggaacag gttcatctct gcgtggggtt tgagagcgtt
     3541 gccgccgggc ataaggaccg ctatgcggtc tatgcactga atgcggtgct aggcggcagt
     3601 gtcagctcga ggctatttca ggagatccga gaaaaacgcg gtttgtccta ttcaatctac
     3661 tcgtttttgt ccggctattc agacagcggc acaattacgg tatacgccgg gattcgcgca
     3721 cgagaggttg agcgggttct cgatctggtc tgccgcgaaa ttcgaaaact ggccagacat
     3781 ggtatcgatg gtcacgagct gaaacgcacg aaggatcaaa tgaagggtag tctcatgctg
     3841 agcttggaga gctcacatag tcggatgaac aagcttgcca aggacgagct gattgccggg
     3901 acacacacga ccctggagga tttgattgcg gagatcgatg gcgtaactga gcaacaagta
     3961 tttcagaccg cacagaatct gttcatcggc gacaaaatcg cgttgaccgg cttgggtcct
     4021 ctctcctcac gtcaagtgga ggggttgagc gcgaaatttt cctaactgcc gttgccgcac
     4081 aggaaagcca atctattgaa atctcacaac agactgttat gtattgacat ttgggcacac
     4141 atttctctag agtcgaatcc atcaaacatg tccttgacgg gacatcgcag tttcagtacc
     4201 gttccgatac cggccggacg gcttcgcggt tcgtttttgg tacgcaggct attgaagaac
     4261 gaaaattttc caaaggcttg atttagcgat atccagcatt ggcgacgaat aacgtctcat
     4321 tggactgttg ggctcaattt gacgaaggag ggacgcgtat gaaaaaggct gtcgttctag
     4381 ccgccg
//
LOCUS       DATNDD010000038         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1120441, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000038 DATNDD010000000
VERSION     DATNDD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120441"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VN647_00840"
     CDS             <1..1611
                     /locus_tag="VN647_00840"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWG95598.1"
                     /translation="RAEELTLYGRDKAKVSLQVLDRLTTVPKGRYVLVTAINPTPLGE
                     GKTTTSIGLAMGLSRLGHRTALTLRQPSLGPVFGMKGGGTGGGHAQVAPMEDINLHLT
                     GDAHAVAASHNLLSAFLDNHLFHGNTLSIDPQRTTWPRTLSISDRALREVLLGEKPDR
                     RPGQFVITEASEVMAALALARDQQDLRERLSRILVGLTKAGTPVTAEELRCVGSMAVL
                     LKDALMPNLVQTLEGTPAFVHTGPFGNIAHGNCSVISDAIALRCADFVITEAGFGSDL
                     GAEKFFNIKCRASGFAPTVAVVVATLRALKLHGGGGVAKAGVALPSSLTGPNREALSK
                     GIANLEQHVANVLAHGVPVVVAVNAFKDDPQDELEWVREQSLKMGAADAAVSTHWADG
                     GKGAEELAVAVIKASERPARFNHLYEVSWPIREKIETIATRMYGADGVTFEPEAERQI
                     ETAEALGYGGVPVCMAKTPLSLSHDPGLKGRPTGFTVPIRELRILAGAGFVTAVCSGI
                     QLMPGLPKKPAGERINFDPKSGKIVGLS"
     gene            complement(1615..>2191)
                     /locus_tag="VN647_00845"
     CDS             complement(1615..>2191)
                     /locus_tag="VN647_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWG95599.1"
                     /translation="ITDPLKVYQAFQSQRGALQRLHDIIYSELRVELGRHDLIEIVSS
                     TRADIMKAVTERANEKASVYGIEIQDVRIKRADLPEQNEKAVFARMQAERERQAKQYR
                     AEGAEEAQKIRSEAEKDREIILAQAYKESEELRGSGDAKAFRIYADAYRQDQKSFEFT
                     RSMEAYKSAFKEGSTLVMSPDSEFFRYLKQR"
BASE COUNT          462 a          595 c          645 g          489 t
ORIGIN      
        1 cgtgccgaag agctcactct ctatggacgg gacaaggcca aagtttcgct acaagtactg
       61 gatcgcctga cgactgtccc gaaggggcgg tatgttctcg tcacggcaat taacccaaca
      121 cctttgggag agggaaagac cacgacatcg ataggccttg ccatgggact ctcgcggcta
      181 gggcatcgaa ccgcgcttac cctcagacag ccatcgctcg gtccggtgtt tggaatgaag
      241 ggaggaggga ccggtggggg gcatgcccaa gtcgctccga tggaagatat caatcttcac
      301 ctgaccgggg atgcccacgc agtggctgcc agccacaatc tgctctcggc gttcctcgat
      361 aatcatctgt tccatggcaa tactctctcg atcgatcctc aacggactac gtggcctcgg
      421 actttgagta tcagtgaccg tgctctccgg gaggtcctgt tgggagaaaa accggacaga
      481 cgtccagggc aattcgtcat caccgaggct tcagaagtaa tggcggcgct ggcattagcg
      541 agggatcagc aggatctccg cgagcggctc agccgaatac tcgttggact gacgaaggcc
      601 ggtacgccgg tcacggccga agaacttcgt tgcgtgggat cgatggctgt tctcctaaag
      661 gacgccctca tgccgaatct cgttcaaact cttgaaggga ctccggcatt cgtccatacg
      721 gggcccttcg gcaacatcgc gcatggcaac tgttcggtca tttccgatgc gattgcgctg
      781 agatgtgccg actttgtcat aacagaggcg ggcttcggca gcgatcttgg agcggaaaag
      841 ttttttaaca tcaagtgtcg tgcatcggga tttgcaccga ccgtggcggt ggtcgtggcg
      901 acattacggg cgctgaaact ccatggcggc ggaggagttg ccaaggcagg cgttgccttg
      961 ccttcgagtc tgacgggtcc gaatcgggaa gctctctcga agggaatcgc caatctggag
     1021 cagcatgtcg ccaatgtcct cgcacacggg gtaccggtcg ttgtcgccgt caatgccttc
     1081 aaagatgacc ctcaggacga attggagtgg gttcgggaac agtcgctcaa aatgggggcg
     1141 gcagacgcag cggtttcaac acactgggcc gacggaggga agggggctga agagctggca
     1201 gttgccgtca tcaaggcatc tgaaaggcca gctcgcttca atcatctgta cgaagtgtct
     1261 tggccgatca gggaaaagat cgaaaccatt gcgacccgca tgtacggcgc agacggagtt
     1321 acatttgaac cggaggccga acgtcaaatt gaaacagcag aggcattggg gtacggcggc
     1381 gtacccgtct gtatggcaaa aaccccttta tcgctgtcac acgatcctgg gctgaaggga
     1441 cgaccgaccg ggtttacggt gccgatccga gagttgcgga ttctagccgg agcgggattt
     1501 gtgaccgccg tctgttcagg cattcagctc atgccgggat tgcccaagaa gccggccggt
     1561 gagcggatca atttcgaccc caaaagtgga aagattgtgg gtctttcctg aagctcagcg
     1621 ttgtttgagg taacggaaga attctgagtc cgggctcatc accaacgtcg agccctcttt
     1681 gaacgcactc ttgtaagctt ccatcgagcg ggtaaactcg aaagacttct ggtcctgccg
     1741 gtaggcatcg gcgtagattc gaaacgcttt ggcatccccg ctgccgcgaa gttcttcaga
     1801 ttctttatag gcctgggcga ggataatttc acggtctttt tcggcttccg atcggatttt
     1861 ctgtgcttcc tccgctcctt cagcgcgata ttgcttggcc tgccgttccc gttcggcttg
     1921 catgcgcgca aagaccgcct tctcattctg ttccggcaag tcggcacgct tgatccgcac
     1981 atcctgaatt tcaatgccgt acaccgaagc cttttcgttc gctcgttcgg tgaccgcctt
     2041 catgatgtcg gctctcgtgc ttgagacgat ctcaatcagg tcgtgtcgac ctaactccac
     2101 gcggagctca gaatagatga tgtcgtgcag ccgttgaaga gcaccgcgct ggctttggaa
     2161 cgcctgatac accttcaagg gatcagtgat t
//
LOCUS       DATNDD010000039         9103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2267225, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000039 DATNDD010000000
VERSION     DATNDD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267225"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN647_00850"
     CDS             complement(<1..407)
                     /locus_tag="VN647_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG95600.1"
                     /translation="MNEGTGYAIEILLVEDNIGDVRLTKEALSEAKVPNRLHVAQDGV
                     AALQFLRKEQPYSDSPHPDLVLLDLNLPKKSGLEVLAQIKQDPALREGLINSRFHSEI
                     CLWGGHQGESAGSGAFSWGTPTVHWPTAALWTHA"
     gene            complement(536..934)
                     /locus_tag="VN647_00855"
     CDS             complement(536..934)
                     /locus_tag="VN647_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG95601.1"
                     /translation="MRSLPTVFADPTQLIQVFQNLIGNALKFSSERPPVIRISACRLL
                     DAKGSPQWRFSVQDNGIGFDSQYSERVFAIFQRLHTRSEYPGNGMRLAIVNKIVERHG
                     GRIWVESTPGVGTAFFFTLNGPLAEHSTAA"
     gene            complement(871..2316)
                     /locus_tag="VN647_00860"
     CDS             complement(871..2316)
                     /locus_tag="VN647_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG95602.1"
                     /translation="MAIWTLWQPAYFVEGLTKVSTAGLSIVTAILLVKLVPQALPWPS
                     PAQLEKVNEKLRSEIAQRLRVEAELHEASDDLKALIEKRTTELAVETVERRRSIEVLQ
                     ENKAQFKTLAEVAPVGIFHCDRDGFIKYSNERLIEMIGVTSQEEVGTAWGHALHPDDH
                     DRVLAAWAHTVETGQPFRSEHRFVHPDGSVTWVLGQASTVPGENGTVMGFVGTLTDVT
                     EDKAKEEQFRLVVESAPCGMLMVDSEGRITLINAQIEALFGYSRSELIGCPVEQLIPA
                     RFRNTQPEMRETFGHAPSVRSMGVGRDLFGLRKDRTEFPVEIGLNPIQTEDDVFVLAT
                     IVDITERKHAERQLEQQAAELSRSNHELEQFAYVASHDLQEPLRMVSSYCGLLDRRYK
                     GKIDQDADEFIQFAVEGATRMKDLIDDLLTYSRVGRKGAAPVAVEATKSVRAAVANLQ
                     IAITEAGAVIHCVRYPPYSLIQLNSFRYFKI"
     gene            complement(2649..3251)
                     /locus_tag="VN647_00865"
     CDS             complement(2649..3251)
                     /locus_tag="VN647_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG95603.1"
                     /translation="MTSHDLQEPLRMVSSYCGLLARRYKGKIDQDADEFIQFAVDGAA
                     RMKGLIDGLLMYARVGRQEKLLVLVDAGESMQAALANLEIAVKETRASIVCEALPQML
                     ADPVQLAQVFQNLIGNALKFRGNGRPVIHVAARNPGERGNAPKWIFSIQDNGIGFEPQ
                     FQERVFEIFQRLHTREKYAGNGMGLAITKKSSNAMVALSG"
     gene            complement(3252..3533)
                     /locus_tag="VN647_00870"
     CDS             complement(3252..3533)
                     /locus_tag="VN647_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG95604.1"
                     /translation="MPERFRTTPPGIEELHTPTSHQAGVWKRKEVFGLRKDGTECPIE
                     VGFSPVEFGSGKQTLVMVLDITERKRAEQHLERQTAELSRSNHELEQFA"
     gene            complement(3497..4471)
                     /locus_tag="VN647_00875"
     CDS             complement(3497..4471)
                     /locus_tag="VN647_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG95605.1"
                     /translation="MPRFLGIDSRLLTICSFVAIVLTVSLIISMAIPDSELTFPFVHA
                     AVEALGSFFAIVVAWHSLTAYAANHSEAHRVWIGLAFLNMGILYGFHALVAPGDLSIW
                     FRSCATLIGGALSVLGWLPSQTHRKVPVGWAVIICMSAYSLLGIVSFRNQEILPPMID
                     QREMTWITTCFTVSGGTAFFGSAVWFLRHLKHSSHWEPVRQASCYASIGISEIFFTWS
                     TIWDPMWWWWHGVRLFSYGIVAYSAKMDFDKGLVRQTELAMQAEENARFRLIVESAPC
                     GMLMVGFNGTISMVNHQLEVVFGYDRNELIGHPIDIPCLNDSGRHRLA"
     gene            complement(4535..5665)
                     /locus_tag="VN647_00880"
     CDS             complement(4535..5665)
                     /locus_tag="VN647_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG95606.1"
                     /translation="MGNLACGVAHDFNNLITAINGFADLVVHDLLMDTRAHNRMLQIR
                     KAGQRAMAITQHLLALGKTTTKDPAIVDMNLLLTDMERMIQLMIGVRRKVTIVLNPFL
                     GRVKGNPRELEQALLILSMNIAQGREEDVHLQIETRTWSVDGKDAKAMGLIHRGPFIA
                     IAVRAISPGESMPKSSAPQCLTFRENEAIGEDEEKFGMSAVLKIVAEHQGYCFVSQDD
                     TEVNEYTLVFPVARIAQDMLAFNNTAELPLRSGSETVLVVDDEDTVRTLIRELLEANG
                     YRVLEASDGTEALRVGEQHKDGIDLLIVDEIMPGLSGHELTAQMAEMHPRLCALPMST
                     VKPQFVGEKPFLMKPFTVDVLLKRVDEALQDGPIGHATALQS"
     gene            5744..6004
                     /locus_tag="VN647_00885"
     CDS             5744..6004
                     /locus_tag="VN647_00885"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HWG95607.1"
                     /translation="MFLKTSLACSFCGKSASEVSKLVAGPKVYICDACVATASRIMSD
                     SGGETSPQRSLLPTRRQRFVGRLSQLLRPFRSGASQPLSVAW"
     gene            6074..6439
                     /locus_tag="VN647_00890"
     CDS             6074..6439
                     /locus_tag="VN647_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWG95608.1"
                     /translation="MKVTDIAFTVYPVTDLKRARQFYETTLGLKESRFFGNEKQGFVE
                     YDIGPGTLAIGIGAPEWKPSRGGGSAALEVDDFNGAMNRLRECGCTFTVEPMETPVCH
                     MAVVLDPDGNSVMIHQRKT"
     gene            complement(6593..6778)
                     /locus_tag="VN647_00895"
     CDS             complement(6593..6778)
                     /locus_tag="VN647_00895"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWG95609.1"
                     /translation="MQANGLRSPFQKESGLYHFARMLDKIRLHQAGQLPEECHPNFGL
                     HAGLDGHLCGVERIRHE"
     gene            7034..7756
                     /locus_tag="VN647_00900"
     CDS             7034..7756
                     /locus_tag="VN647_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95610.1"
                     /translation="MKLPDRIYHLAEAANWPSIQRHGLLSAGRLMEVAGLTKADRDRL
                     GRTQRPAHTELCNGVQIRDQRPMSPTALAHCLRDMEPADWYAMINARVFFWLDVERLN
                     RQKAACEPRPQVVMAVDTAALVAAYEERVAVTPINTGNARRKPARRGVATFVPLAEWG
                     RSGWASEAAASGISSRGRSHQPVELTVIDAVPDIMRFVVGIVALSSGQSFEERKRVGK
                     VDAALINSVGIRRMDDLTQSPV"
     gene            8182..8859
                     /locus_tag="VN647_00905"
     CDS             8182..8859
                     /locus_tag="VN647_00905"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HWG95611.1"
                     /translation="MTRQAAYNISDSTFAFIGCSEVQESLGQQAEDEKELAELVEEVP
                     LDSIHFHTHSYFLRHRFIERAYPNDFSQWVVMQVGDHVLGERLAVVDPFDYPNLEDLR
                     EEIISIIDDHLSRMSTIPRVVFGEPFHFKRSRILEVPIGLEARSLAEFRQVVAEVDVS
                     AIYFHMFEAHFRLGREESDFSAWIRTGLGLHELADRIRSINPYLGSLERLRSSLITVC
                     DEFLSRV"
     gene            8869..>9103
                     /locus_tag="VN647_00910"
     CDS             8869..>9103
                     /locus_tag="VN647_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWG95612.1"
                     /translation="MLNQDPLWYKDAVFYELHVRAFYDSNDDGIGDFAGLIEKLDYLE
                     WLGVDCLWLLPFYPSPLRDDGYDVADFTTIAPSY"
BASE COUNT         2072 a         2492 c         2435 g         2104 t
ORIGIN      
        1 gcgtgtgtcc acagagctgc agtgggccag tggacagtgg gtgtacccca cgaaaaggct
       61 ccagaaccag cggattcccc ctggtggcca ccccacaaac agatttccga gtgaaaacgt
      121 gaattaatca gaccttccct aagtgccgga tcttgtttga tttgcgccaa tacctctagt
      181 ccgcttttct taggtaggtt gagatccaag aggacgagat ccgggtgagg gctgtcacta
      241 tacggctgtt cctttctgag aaactgcagc gcagccacgc catcttgcgc tacatgcaga
      301 cgattgggaa ctttggcttc tgacagcgcc tctttggtca ggcgtacatc accgatgttg
      361 tcctcaacca acagaatttc aatcgcatac ccagttcctt cgttcatcgt ctttcctccc
      421 tgacccttga ttgggacata cctcaactga agttttctct cacccgcagt gatgtcacac
      481 gaagagcgcc ttctatcgtt ccagagtgac ttgtcgattt tcatttatcc ccgatttatg
      541 ccgccgtgga gtgttcggcc aacggaccat tcagtgtaaa aaagaaggcg gtaccgactc
      601 ctggcgtcga ttccacccaa atcctgccgc catgacgctc gacgatttta ttgacaatcg
      661 ccagtctcat accattgcct ggatattcag accgtgtgtg caatcgttga aagatggcga
      721 acactcgttc gctatactgt gagtcaaatc cgatcccatt gtcttgaacg gaaaatcgcc
      781 actgcgggga gcccttggca tctaatagac ggcatgccga aatgcggatg accggtggcc
      841 gttcgctgct aaatttaaga gcattgccga tcagattttg aaatacctga atgagttgag
      901 ttggatcagc gaatacggtg ggtagcgaac gcagtgaatg actgcacctg cttccgtaat
      961 ggcgatttgc aaattggcga ccgccgcccg tacgcttttc gttgcctcaa cggcgacggg
     1021 ggccgcacct tttcgtccga cccgtgaata cgtcaacagg tcgtcgatca agtccttcat
     1081 ccgtgtggcc ccttcgacgg caaactggat gaactcgtcg gcgtcttgat cgattttccc
     1141 cttgtaccga cggtccaaaa gcccgcaata gctcgatacc atccgcagcg gctcttgcaa
     1201 atcgtgcgac gccacatagg cgaactgttc cagctcgtgg ttggagcggc tcaattctgc
     1261 cgcctgttgc tcgagctgcc gctcggcgtg ctttcgctca gtgatatcta ctattgtcgc
     1321 caggacaaat acatcatctt ctgtctgaat gggattgagt ccgatttcga ctggaaattc
     1381 ggtgcggtct tttcgcaatc cgaacaagtc ccgacctacc cccatggatc ggacactggg
     1441 agcatggccg aaggtctccc gcatttcggg ttgggtattc cggaaccgag ccggaatcaa
     1501 ttgttcaacc ggacacccaa tcaactcgct ccgcgagtac ccaaacagtg cttcaatttg
     1561 ggcgttgatc aaggtaatcc gcccttctga atcgaccatc agcatgccac aaggggccga
     1621 ttccaccacg agtcggaatt gttcttcttt tgccttatct tcagtaacgt ccgtcagcgt
     1681 tccgacgaaa cccatcactg ttccgttctc gccaggcaca gtagaggctt gtcctaatac
     1741 ccaggtcaca ctgccgtcgg gatgcacgaa ccggtgctcc gatcggaagg gttggccggt
     1801 ttcgaccgtg tgtgcccatg cagcaagaac tcgatcatga tcgtcaggat ggagagcgtg
     1861 cccccatgcc gttcctactt cctcctgtga ggtcacgccg atcatctcga tcaagcgttc
     1921 gttggaatac ttgatgaaac cgtctctatc gcagtggaat atgccgacgg gagcaacctc
     1981 cgccaatgtt ttgaattgtg ccttgttttc ctgcagaact tctatcgacc gcctacgctc
     2041 gacggtttcc actgccaatt ctgtagtgcg cttttcaata agtgctttca agtcgtccga
     2101 cgcttcgtgc aactcagctt ccactcgaag tcgttgtgcg atctccgatc gcagtttttc
     2161 attgaccttc tccaattggg cagggctcgg ccatgggaga gcttgcggca caagttttac
     2221 caagagtatc gccgtgacga tggacaagcc cgccgtcgac actttcgtca atccttcaac
     2281 gaagtaggcc ggttgccata gagtccatat ggccatcaga tgggtggtcc cgcacgcgaa
     2341 gataaatgca ctgaactaag tgaagcatcc agccaaagct cagatcttcg cgtttccgca
     2401 caaaattggc gagtatgaat ggaatggagt aatacgcaag gacgacggcg ctgtcagcga
     2461 tcatatgaag ccagatcagg ccggactccc agaggagaca ctggccgtgc ggaagaaatc
     2521 cgggagggat ggaccaaggc agaagatcgt tcattcacca ccttatgttc aggcagcggc
     2581 gttgaaccgt ttcaaaacat gcccgcgcaa cgtgaagaag aagatcctac cggaacctgg
     2641 agttgattct acccagatag tgccaccatg gcgttcgatg attttttcgt aatcgccagc
     2701 cccatgccgt tgcccgcgta cttttcccgt gtatgcaatc gttgaaagat ctcgaacacc
     2761 cgctcctgga attgcggttc gaagccgatc ccattgtcct gtatcgagaa gatccacttc
     2821 ggtgcatttc ctcgctcacc cggatttcga gccgccacgt gaatcaccgg gcggccgtta
     2881 cccctaaact tcaacgcatt accgatcaga ttctgaaaca cctgagccag ctgtaccgga
     2941 tcggccagca tctgcggcag tgcctcgcaa acaatcgacg ctcttgtttc cttgacagct
     3001 atttccagat tggccagagc cgcctgcatg ctctcgcccg catcgaccaa gactaagagc
     3061 ttctcttggc gaccgacccg tgcatacatc agcaaaccgt cgatcaaacc cttcatgcgt
     3121 gcggccccat cgacggcaaa ttggatgaac tcgtccgcgt cctgatcgat ctttcctttg
     3181 taccggcggg ccagaagccc gcaatagctc gacaccatcc gcaacggctc ttgcagatcg
     3241 tgcgacgtca cctaggcgaa ctgttccagt tcgtggttgg agcggctcaa ttccgccgtc
     3301 tgtcgctcga gatgctgctc ggcgcgtttc cgctcggtga tatccaacac catcacaaga
     3361 gtttgtttcc cggagccgaa ctcgacagga ctgaaaccca cttcgatcgg acactctgtt
     3421 ccgtccttgc gcaacccaaa cacctctttc cttttccaaa cacctgcttg atgggacgtg
     3481 ggagtatgca gctcctctat gccaggcggt gtcgtccgga atcgttcagg caagggatat
     3541 cgatggggtg tccgatcagc tcatttcggt cgtacccgaa gaccacttcg agctgatggt
     3601 tgaccatcga tatcgtgcca ttgaatccca ccatcagcat gccgcatggc gcggattcca
     3661 caatcaatcg gaaccgagca ttctcctccg cctgcatagc gagctcggtt tgccgaacca
     3721 atcccttatc gaaatccatt tttgcgctgt aggcaacaat gccgtaggag aagagtcgaa
     3781 cgccatgcca ccaccaccac atgggatccc atatggtcga ccaggtaaag aagatttcgg
     3841 agatgccgat ggatgcataa caggacgcct gccgcaccgg ttcccagtgc gacgaatgtt
     3901 tcaggtggcg gagaaaccaa acggcactcc caaaaaacgc ggtcccgccg ctgacggtga
     3961 agcacgtcgt aatccatgtc atctctcgct gatcgatcat gggagggagg atctcttgat
     4021 tcctgaagga aacgattccg aggagggaat aggccgacat acagatgatc actgcccatc
     4081 cgactggaac cttgcgatgc gtctggctag gtaaccaccc aagcaccgac agcgcgccac
     4141 cgataagcgt cgcacagctc cgaaaccaga tcgataggtc ccctggagcg acaagcgcgt
     4201 gaaacccata gaggataccc atgttcagga acgctaaccc tatccatacc cggtgagctt
     4261 cactgtgatt tgccgcatag gcagtgaggg aatgccaggc aacgacgatc gcgaaaaatg
     4321 aacccaacgc ttccacagcg gcgtgcacaa agggaaatgt caattccgag tcgggaatgg
     4381 ccatcgaaat aatcaaggag acggtcagaa cgattgccac gaatgagcaa atcgtcaata
     4441 accggctatc gatacccaag aaccgaggca tggaatccca cccatgaaga ccacctagtg
     4501 acaggtggtt cggattacaa gaaatgtgtt gccgctacga ttgcagcgct gtcgcatgac
     4561 cgataggacc atcctgtaga gcctcgtcaa ctcgcttcaa taggacgtcc acggtaaacg
     4621 gcttcataag gaacggtttc tcaccaacga actggggctt gacggtcgac atcggcaacg
     4681 cacacaaccg cgggtgcatc tcggccattt gggccgtcaa ctcgtgaccg ctcaggcccg
     4741 gcatgatctc atccacgatg agcaggtcga ttccgtcctt gtgctgttcg ccgacacgaa
     4801 gagcttcggt tccatccgat gcctccagta cgcggtaacc gttcgcctcg agcaactcac
     4861 ggatcaacgt ccgcacggta tcctcatcgt cgacgaccaa cacggtttca gagccggagc
     4921 gtagcggcaa ttcggcagta ttgttgaatg ccagcatatc ttgggcgata cgcgcaaccg
     4981 gaaagacgag cgtgtactcg ttcacctctg tgtcatcttg ggagacgaaa caatacccct
     5041 gatgctccgc aacaatcttc aagaccgccg acatgccgaa cttctcttcg tcttcgccga
     5101 tggcttcgtt ctctcgaaac gtcaaacatt gcggggccga actctttggc atggattccc
     5161 caggagaaat cgcacgaacc gctatggcaa taaacgggcc gcggtggatt agacccattg
     5221 ctttcgcgtc cttgccgtcg acactccaag tccgtgtctc aatctgcagg tgtacatctt
     5281 cctcgcgacc ctgggcgata ttcatcgaca ggatgagcag cgcctgttcc aactctctgg
     5341 gattgccctt gacacgtcct aggaatggat tcaaaacgat cgtgactttg cggcgtactc
     5401 caatcatcag ctggatcatg cgttccatgt ccgtcagcag cagattcatg tccacgatcg
     5461 ccgggtcttt cgtcgttgtc tttcctagag cgagcaagtg ctgagtaatc gccatggctc
     5521 gctggccggc ttttcgaatc tgcagcatgc ggttgtgggc tcgtgtgtcc ataagcagat
     5581 catgtacgac aagatcggca aacccattga tggcggtaat gagattgttg aagtcatgcg
     5641 caactccgca ggcaagattc cccatcgcgg cgcgctccgc ccatgaacct gcgcgtgagg
     5701 tgttaactgg tacaatccag aacattaata gaggaggtgc actatgtttt tgaaaacttc
     5761 acttgcctgt tctttctgcg ggaaaagcgc ctcggaggtc tcaaagcttg ttgcggggcc
     5821 gaaggtctat atttgcgatg cgtgcgtggc gacggcgagt cgcatcatga gtgactcggg
     5881 cggggagacg tctccgcaac gttcgctcct gccgacacga cggcaacggt ttgtcggccg
     5941 actttcacaa ctgctcagac cgtttcgatc cggcgcttcg cagccgttat ctgtagcatg
     6001 gtgaatcgga tccgacaact actgtggacg ccttcacatc tatgggagag cagagcaata
     6061 ggagaacacc gacatgaagg ttacggacat cgcatttacg gtctatcccg tcactgactt
     6121 gaaacgtgcc cgccaattct atgagacgac cttgggactt aaggagtcgc ggtttttcgg
     6181 gaacgaaaag cagggctttg tcgaatatga cattggaccg gggacgctcg cgatcggcat
     6241 tggagcgccg gaatggaaac cctcacgtgg tggaggatct gcagctctcg aagtagacga
     6301 tttcaatgga gcgatgaacc gtcttcgaga gtgcggctgc acgtttaccg tagagcccat
     6361 ggagactcct gtgtgtcaca tggcggtagt tttagatccc gatgggaatt ctgtgatgat
     6421 tcaccaaaga aaaacgtgat gaagacgtcg cttctccgaa accgatggca tgggtgaggc
     6481 aaggacggac ctgcttaacg aggaaccgtc tttccttgaa ccggttgtgc atgtgaagga
     6541 acataagaca tgcgttgttt tccagagcgc gaggagggta tcgaggttac cctcattcat
     6601 gtcgtatccg ttcaacgccg cataagtgac cgtccaatcc tgcatgtaac ccgaagttgg
     6661 gatgacactc ttccggcagc tgcccggctt gatgaagccg aatcttgtcg agcatcctgg
     6721 cgaaatggta cagcccgctt tccttctgaa agggactacg aagaccgttg gcctgcatat
     6781 gtgacctccg ttgcgactgg ttcgattatg cgaggattcg tactcggttt cagtttcccc
     6841 atgatttttt ttgcgaacga tatccggtag tggaacaatc agtcattatg agtgtaaaac
     6901 gaggaagcgc gttctagatg gaacgcgaat tgtaagatgt ccctccgaac tgtcagaatt
     6961 actcggcgag gacaagttcg gtcacactat ttgggttggt gtccattcag tcacgaaagt
     7021 gagaaaattc catatgaagt tgccggatcg gatttaccat ttggcggaag ccgcgaactg
     7081 gccgtcgatc caacgtcacg ggttgctatc tgccggccgg ttgatggaag ttgcgggact
     7141 gaccaaagcg gatcgagatc gactggggag gacgcaacgg ccggcacata ccgaactgtg
     7201 caatggggtg cagattcgcg atcagcgtcc gatgtcgcca accgcactgg cccattgtct
     7261 ccgtgacatg gaaccggccg attggtatgc gatgatcaac gcgcgcgtgt tcttctggct
     7321 cgatgtggag cgactcaacc gccagaaagc tgcctgtgaa ccgaggccgc aggtcgtgat
     7381 ggcagtggat acggctgctt tggttgccgc gtatgaggaa cgagtggcag tgactccgat
     7441 caataccggg aatgcgcgtc gaaagccggc gcgacgtgga gttgccacgt tcgtcccgtt
     7501 agcggagtgg ggcaggtccg gatgggcgag cgaggcggca gcatcgggca tctcttcacg
     7561 agggaggtcg catcaaccgg tcgagttgac cgtcatcgac gcagtgcctg acattatgcg
     7621 tttcgttgtc gggatcgttg ccttatcctc cggtcagtcg tttgaggaaa ggaaaagggt
     7681 gggcaaggtc gatgccgctc tgatcaactc agtggggata cggagaatgg atgatctcac
     7741 gcaatctcct gtgtaatgta gagctttccc gagctgtact catctaagat agagaggtaa
     7801 gttaatccgg tagtcggctc ggtactgaag aaggacacca cgtagattgt cattgtgctg
     7861 tatccaggaa tgaccacgct tccgcggccg cagccctcag ttcctggccg cttgacaaac
     7921 ggggagcact acatgacatt gagtgggaat gtgctgagaa ttgcagtctg attgtgcgtc
     7981 atagcgcaac ctctggaacc aagattagac agctttccgg cgagtgagcg acaacattct
     8041 gcgagtatcg cggccgcagg atcaaaacgc gtagtcttag ggttgacaca taccgcagag
     8101 ttccagttag agtcaccgtg aacagctatg aatcataaga atgtgtcttc aaataggcat
     8161 agatcgtaat ttatacctca tatgacccga caggctgcat ataacatctc cgattccaca
     8221 tttgcattca tcggatgcag cgaggtgcag gaaagcctgg gacaacaggc ggaggatgag
     8281 aaagaacttg ccgaactcgt cgaagaggtc ccactcgatt ccattcattt ccatacccat
     8341 agctattttc tcaggcatcg cttcatcgag cgggcctatc cgaatgattt ttcccaatgg
     8401 gtcgtgatgc aggtcggaga tcatgtgttg ggcgaacggc tcgccgtggt cgatccattc
     8461 gactatccga acctggaaga tctgcgggaa gagattattt cgattatcga cgatcatctg
     8521 tcgcgaatgt ccactattcc ccgtgtggtg ttcggcgagc cgttccattt caagcgttcc
     8581 aggattctgg aagtgccgat cggtctggaa gcgaggtccc tggcggaatt tcgacaggtg
     8641 gtggccgaag tcgatgtcag cgcgatctat ttccatatgt tcgaagcgca ttttcggttg
     8701 gggcgcgaag aaagcgactt ctcggcctgg attcgaacgg gcctcggctt gcatgaactg
     8761 gctgatcgta ttcgctccat caacccgtac ttgggaagcc tcgagcgcct gcggtcgagc
     8821 ctcatcacgg tgtgcgacga attcctgtcg agagtatgag ggcatgggat gctgaaccaa
     8881 gatcccctct ggtacaagga cgccgtgttt tacgagttgc acgtgcgggc cttctacgac
     8941 agcaatgacg acggcatcgg agatttcgcc ggcctgatcg agaagctcga ttatctcgaa
     9001 tggctgggag tggactgtct ctggctgctg ccgttttatc cgtcgccatt gcgagatgat
     9061 ggctacgatg tggcggactt caccaccatc gcgccttcgt acg
//
LOCUS       DATNDD010000040         3203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_552724, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000040 DATNDD010000000
VERSION     DATNDD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552724"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VN647_00915"
     CDS             complement(<1..424)
                     /locus_tag="VN647_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG95613.1"
                     /translation="MMAVRKVVHIITRLDQGGSARNTMLTVLGHDRTQFEPVVVTGQA
                     DMWNAQGGLTATIENLMILEKESIRYHMVPSLVRHIGPKADLTALWRLIVLLRQEQPY
                     IVHTHTSKAGVLGRLAARITGAPMIVHTPHGHVFYGHFG"
     gene            complement(442..1665)
                     /locus_tag="VN647_00920"
     CDS             complement(442..1665)
                     /locus_tag="VN647_00920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWG95614.1"
                     /translation="MNILLLAEVSAERVLGGAERVLRNQALGLAMLGHHVELLTRAPE
                     HASEGVLEMAGIREWRYSVSRKHEAAFLWSSVRRSVERFDHLRAFEPLDAVIIHQALA
                     GLGPILARRHAASRWIYMCHSLAHEEYETRQDPTCSTLANLRRHANLRARRSVEHAVM
                     SRCHHVAVLSQFMRQRVVGTHNISVDRLALIPGAVDPHAFVPLKDRLPIKSALNLPAD
                     RTILFTVRNLVPRMGLDNLLRAIEMLNRERHELMLVIGGEGPLREQLQADIRQKGLSE
                     VVRLIGFVPESRLGQYYQAADLVLMPSLQLEGFGLVMVEAMACGTPVLGTPVGAIPEI
                     LNQVDPILVAEGVDGRSIGRALERVLRRLQEPGEASRLAKKGRALIERRYNWKQHCNE
                     LVSLLNGPRELRLAA"
     gene            complement(1662..>3203)
                     /locus_tag="VN647_00925"
     CDS             complement(1662..>3203)
                     /locus_tag="VN647_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HWG95615.1"
                     /translation="WLVRDRLGIKPLYYATRQCETGRGPSVIFSSEIPSLLEALPDWR
                     IRTEAVAEYLMHLYVPGPGTMIAGVHQLRPGEAMSISDGRMKLLRYYRPEEAFDDHEP
                     WTIKEAGREVVHTFEEAVQAHLVSDVPLGLFLSGGIDSASLLAMMAKEQNEPVKTFSI
                     GYEHPSDQSFSELDSARFLADHFKTVHSETILRPDAVALLGKVVEGMGEPFADSSAIP
                     TYLISEVARRTVTVALSGIGGDELFGGYPRYLGLRTAAQYQRIPHMLREHMARWSRHI
                     GEHGQARDQAARLKRFLGDGHLPLPEQYRRWTTFIPSEWDGTLWGHRLEAMFLNNRPL
                     GRAEVLFDQWPSSDPADCAMGVDLQSYLPDDLLRMADRMSMVHSLELRVPFCDHQLLA
                     AALRIPSQIRLTGWQLKGFMRRMLRGLLPDRILRAPKQGFMVPMARWLREDLRELAHD
                     LLTDDVIKDRGYVKPAYVRWLLEQHESGRRNLSDQLYALMVLELWHRGLRADSSERRA
                     MMATS"
BASE COUNT          732 a          978 c          840 g          653 t
ORIGIN      
        1 aaccgaagtg gccatagaac acatgcccgt gtggtgtatg aacaatcatg ggagcgcccg
       61 tgatccgcgc ggccagcctc cccagcactc ccgctttgga tgtgtgcgtg tggacgatgt
      121 agggctgttc ctgtcgtaac aggacgatca gtctccacag cgccgtcaga tcagcctttg
      181 ggccgatatg ccgaaccagc gacgggacca tgtggtaacg gatcgactct ttttccaaga
      241 tcataagatt ttctatcgtt gcagtgagtc ccccctgcgc gttccacata tccgcttgtc
      301 cggtgaccac aaccggctcg aactgagtgc ggtcatgccc caacaccgtg agcatggtgt
      361 ttcgagcaga accaccctga tccaaacgag tgatgatatg aacgaccttt cgaacagcca
      421 tcatagtttt tcaatggcct gctacgccgc gagccgtaac tccctcgggc cattcaacaa
      481 gctgaccagc tcgttgcaat gttgtttcca attgtaccga cgttcaatca atgcccgacc
      541 cttttttgcc aaacgacttg cctcgcctgg ttcttgtaat cgtctcaaga ctcgttcaag
      601 cgcccgtcca atcgatcgac catcgactcc ttccgcgacc aggatgggat cgacttggtt
      661 caagatctcc ggaatcgccc caaccggtgt cccgagcacg ggtgtcccgc aggccattgc
      721 ttcgaccatc accagaccga aaccctctaa ctggagactc ggcatcagga caaggtccgc
      781 ggcctggtag tattgaccga gccgggattc gggaacgaac ccgatcagac gcacgacttc
      841 gctgagtcct ttctgccgta tgtctgcttg aagttgttca cgcaaagggc cttctccacc
      901 gatcaccagc atcagctcgt gccgttccct gttcagcatc tcgattgcac gcaacagatt
      961 gtcgagcccc atcctcggca caaggtttcg aactgtaaag aggatcgtac gatcggctgg
     1021 aaggttgagc gctgacttta taggtagtcg gtcctttaac gggacaaagg catgtggatc
     1081 aaccgccccc gggataagtg ccaaacgatc gaccgaaata ttgtgggtcc ccaccacccg
     1141 ctggcgcatg aattgactga ggacggctac atggtgacat cgggacatca ccgcatgttc
     1201 taccgagcgc ctagcccgaa gattggcatg tcgccgcaag ttagccaggg ttgaacaagt
     1261 tggatcttgc ctcgtctcat attcctcatg agcaagagaa tggcacatat agatccatcg
     1321 ggatgcagca tgacgacgag ccagaatcgg gccgagtccg gccagcgcct ggtgaatgat
     1381 gacggcatcc agtggttcga acgcgcgtaa gtgatcgaac cgctcaaccg agcgacgtac
     1441 tgaagaccag agaaacgccg cttcgtgctt gcgactgacc gaatagcgcc attcgcgaat
     1501 tccggccatc tccagcacgc cctctgatgc atgctcgggg gcccgcgtca gcagctccac
     1561 gtgatgtccc agcatcgcca gtccgagagc ttggttgcga agaacccgtt ccgctccgcc
     1621 gaggactcgc tccgccgaaa cttcagccaa caacagaata ttcatgaggt ggccatcatt
     1681 gcccttcgtt cagaggagtc ggcgcgcaaa cctcgatgcc acagctccag aaccatcagc
     1741 gcatacaatt gatctgaaag gtttcggcgg ccgctctcat gttgctccag caaccaccgc
     1801 acgtaagccg gtttgacata cccgcggtcc ttgatcacgt catccgttaa cagatcgtgt
     1861 gccagctcgc gaaggtcctc tcgtagccat cgagccatcg gcaccatgaa tccttgcttg
     1921 ggggcgcgaa gaattcgatc cggcagaagg ccccgcaaca ttcggcgcat aaaccctttc
     1981 aattgccaac cggtcagacg tatttgagag ggaattcgca gggctgcggc caagagctga
     2041 tgatcgcaaa acggaacacg caactcgagc gaatgcacca tactcattcg atcagccatg
     2101 cgcaggagat catcgggcaa ataactctgt aggtcgacac ccatggcaca gtccgccgga
     2161 tcggacgacg gccactgatc gaacaaaacc tcagcccgtc ccaacggcct attgttgaga
     2221 aacatcgctt ccaatcggtg tccccagagg gtaccgtccc attcggaggg aatgaaggtg
     2281 gtccagcggc gatattgctc gggaagcggc agatgcccat cgcccagaaa tcgtttcagg
     2341 cgggccgctt ggtcacgagc ctgcccatgt tcgccgatat gcctgctcca tcgagccatg
     2401 tgctcccgca gcatatgggg aattcgttga tactgcgcgg cagtacggag gcccaaatag
     2461 cggggatagc caccgaagag ctcgtcgccg ccgatccccg aaagcgcaac cgtgacggtc
     2521 cgccgcgcaa cctccgagat gagataggta gggatagccg acgagtcagc aaatggctcc
     2581 cccatgccct ccaccacttt cccgagcagc gcgaccgcat ccggcctcag aatcgtctcc
     2641 gaatgaacag tcttgaagtg atcggccaaa aatcgtgctg agtccagctc gctgaatgat
     2701 tggtcggaag gatgttcata tccgatcgag aaggtcttga ccggctcatt ctgttctttc
     2761 gccatcatgg ccaacaacga tgccgagtcg ataccgcctg agagaaatag ccccaacggg
     2821 acatcgctga ccagatgagc ttgcacggcc tcttcaaatg tatgtactac ctctctgccg
     2881 gcctctttga tcgtccacgg ctcgtgatca tcgaacgctt cttcaggacg gtaataccgc
     2941 aacaacttca ttcgcccgtc cgatatgctc atcgcttcgc ctggacgcaa ttggtgcacg
     3001 ccggcgatca tcgtgccggg ccctggcaca tacaaatgca tcagatactc ggcaactgcc
     3061 tccgtccgga tccgccaatc gggcaaggct tcgagcagag acggaatctc cgaggaaaat
     3121 atgacggaag gccccctgcc ggtttcgcat tgtcgagtcg catagtacag cggcttgata
     3181 ccaaggcgat cacgaaccag cca
//
LOCUS       DATNDD010000041         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1960755, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000041 DATNDD010000000
VERSION     DATNDD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960755"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VN647_00930"
     CDS             complement(<1..1181)
                     /locus_tag="VN647_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWG95616.1"
                     /translation="MAESKGQPNRLRSLRDSVKKITKRLSEGNGEMIHKNDEHAREMD
                     KLRVQIQSMEEEIRRLYQSRYQLDQANKQNEKLVATLQEAKAQIEVLRTEVEKLTAPP
                     SAYAIFSSMNADGTGNIYVSGRKMKVSLHPSIKRNELRKGQEVVLNEALNVIEVKGFD
                     SQGEVVRLKDVLEGGRALVTLHFDEEKVAELGDPLLADRLSVGDHLLYDPRSGYVIEK
                     LPKSEAEELVLEEVPDVDYEHIGGLQKELEQVRDAVELPFLHPQIFSEYKLSAPKGVL
                     LYGPPGCGKTLIAKVVANSIAKKLGHRSGKEIRSYFLHVKGPELLNKYVGESERQVRE
                     VFKKAKERAEDGHPVIVFFDEMDALFRTRGTGISSDIESTIVPQFLSEIDGVERLRNV
                     IV"
     gene            complement(1321..1947)
                     /gene="thiE"
                     /locus_tag="VN647_00935"
     CDS             complement(1321..1947)
                     /gene="thiE"
                     /locus_tag="VN647_00935"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWG95617.1"
                     /translation="MTPVDFRLLLVTDRHQTHGRSLVRVLHDAIKAGVPAIQLRERDL
                     STSDLLSLTQKIQAITTSGSVPLIVNDRVDLTMALNLDGVHLRSDSLPPLPVRQLIGP
                     RRLIGVSTHSVEDVRRANQGGADYVVFGPIFETPSKRPFGSPLGLHLLADVCRRSSIP
                     VLAIGGITRERVHDVRRAGAHGVAVIGALLSRNDIGEAVREFRHALER"
     gene            complement(2063..>2342)
                     /locus_tag="VN647_00940"
     CDS             complement(2063..>2342)
                     /locus_tag="VN647_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWG95618.1"
                     /translation="IRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMN
                     TAIAGAQDPLAMAEAMRYAVHAGRLAYKAGRIPRKLYATASSPIEGML"
BASE COUNT          527 a          694 c          596 g          525 t
ORIGIN      
        1 acgatgacgt tgcgcaatcg ttcgaccccg tcgatctctg aaagaaactg gggaacgatc
       61 gtcgactcga tatcggagga tatgcctgtc ccacgggttc tgaagagcgc atccatctca
      121 tcgaaaaaca cgatcaccgg atgaccgtcc tcggcccgtt ccttcgcctt cttaaacacc
      181 tcgcgtacct gacgctccga ttccccgaca tacttattga gcaactcggg gcccttcaca
      241 tgaagaaagt agctccggat ttcttttccc gaccgatggc ctagtttctt ggcgatcgaa
      301 ttggcgacga ctttggcgat cagtgtcttc ccacagccgg gcgggccgta gagcagaacc
      361 cctttcggtg cactgagctt atactcggag aatatctgcg gatgcaggaa cggaagttcg
      421 acggcatcgc ggacttgctc caattccttc tggaggcccc cgatgtgctc atagtcgaca
      481 tccgggacct cttccaatac aagctcttcc gcctcggact tcggtaactt ctcaatgaca
      541 tagccggaac gaggatcata caacagatgg tcacccacac tcagccgatc agcgagcagt
      601 gggtctccga gttcggccac cttctcttca tcgaaatgga gcgtgaccaa tgcccgtcct
      661 ccttccagca cgtctttgag tcgaacgacc tccccttgac tgtcgaaccc tttgacttcg
      721 atcacgttga gcgcctcatt gagaacgact tcctgtcctt tccgcaactc attgcgctta
      781 atcgaagggt gaaggctcac cttcattttc cgtcccgaca cataaatatt tccggtaccg
      841 tcagcattca tgctggaaaa aatggcatag gccgacggcg gcgccgtcaa tttttcgact
      901 tcggtacgaa gcacctcgat ttgcgccttc gcttcctgca gcgtggccac gagtttttcg
      961 ttctgcttat tggcttgatc gagttggtag cgggattggt agagtcgacg gatttcttcc
     1021 tccatcgact ggatctgaac gcggagcttg tccatctcgc gcgcatgttc gtcgttcttg
     1081 tgaatcatct ccccattccc ctcggacagc cgcttcgtaa tcttcttgac ggaatctcgg
     1141 agggaccgga gtcgatttgg ctggcccttg ctttccgcca tatcgggacg acacgaatag
     1201 ctcgcgacat cgtgcgtaaa cgcgaatagt ctaatgatcg gattctaaca cccgacctag
     1261 gggtggtcaa ctccaccgga cgatctcgcg cgaaactcgt cctcgccgtg gaacgccggc
     1321 tcacctttcc agagcatgcc tgaactctcg gacggcttcg ccgatatcat tacgagacaa
     1381 caatgctccg atcacggcaa ctccgtgagc accggctcga cgaacgtcgt gcactcgttc
     1441 gcgcgtaatt cctccaatgg caagaacggg gatggacgat cggcggcaga catctgccag
     1501 cagatgaagc ccaagcgggg acccgaacgg acgcttcgac ggggtctcaa agatgggacc
     1561 aaacacgaca tagtcggcac cgccctgatt cgcacgtcgc acatcctcga ccgaatgggt
     1621 ggagactccg atcaatcggc gtggtccgat cagctgtcgc acagggagag gaggcaggct
     1681 gtcggatcga agatgcaccc cgtccaaatt gagggccatc gtgagatcaa cccgatcgtt
     1741 aacgatcaac ggaacagatc cagacgttgt tatggcttgg attttttgtg tcaacgacag
     1801 taagtcgctg gtagacagat cacgttccct tagctggatc gccggtacgc cggccttgat
     1861 cgcatcatga aggactctta caagagaacg tccgtgagtc tgatgacgat ccgtcaccag
     1921 aagcaaacga aaatctaccg gggtcatcgg cgggaggata aaaccgaagt gccgactacc
     1981 gagaaatccg agtgaagacc gttcatgttg cacttctcta actgacaaaa ctcacgacgc
     2041 atcactcagc actcaaacta ctttagagca tcccttcgat cgggctactg gccgtcgcat
     2101 agagcttcct gggaatgcga ccggccttgt aagctagacg accggcatga accgcatacc
     2161 gcatcgcttc cgccatggca agcggatcct gcgcaccggc aatagccgta ttcatgagca
     2221 ccgcgtcggc accatattcc atcgctaagg ccgcgtccga cgcggttccg acaccggcat
     2281 cgacgataat cggcaccttg acggtttcca tgatgatttt cagattgtac ggattacgaa
     2341 tt
//
LOCUS       DATNDD010000042         5249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3118532, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000042 DATNDD010000000
VERSION     DATNDD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118532"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VN647_00945"
     CDS             complement(<1..353)
                     /locus_tag="VN647_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWG95619.1"
                     /translation="MTIELNHTIVPAHDKDKSARFFAEIFGLQYEGSSGHFAPVRVND
                     RLTLDFDQDDSFESHHYAFHVDDTEFDAIFDRVRQAGLAYGSHPWDAENRKLNDWNGG
                     RGVYFRDPNGHLLELL"
     gene            complement(343..552)
                     /locus_tag="VN647_00950"
     CDS             complement(343..552)
                     /locus_tag="VN647_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95620.1"
                     /translation="MSEQMRLTMLCVRDGVDAAGEWAQSTLKLYRQSVENPAHFASQS
                     DWKARFDQSMRELAAFIERGIIHDH"
     gene            complement(785..1270)
                     /locus_tag="VN647_00955"
     CDS             complement(785..1270)
                     /locus_tag="VN647_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HWG95621.1"
                     /translation="MTVVIPPKHNNVFNRVANACSRMFGTAGAFWIACGVVAIWALTG
                     PLFNFSDTWQLVINTGTTIVTFLMVFLIQNTQNRDSAALQLKLAEIIRSTQGAHNAMI
                     DLEKLSQTDLDKIRSLYEHLAERARSTEGQGDSDTGTPAVPEEQTETRVIIEAAGKAH
                     Y"
     gene            complement(1487..1918)
                     /locus_tag="VN647_00960"
     CDS             complement(1487..1918)
                     /locus_tag="VN647_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWG95622.1"
                     /translation="MSSTSIKELAHKYAEALNAGEIDRFDDFIADDYINHNPYVKPGL
                     RGIKEFFEGWMRSFPDTEVTVEDVLAEGDMLVGRFTYRATHQGEFLGLPATGRSIRMR
                     SIDIWRVRDGRFVEHWDELNLLEVMHRLGAVSAKGYSTVEI"
     gene            2422..3291
                     /locus_tag="VN647_00965"
     CDS             2422..3291
                     /locus_tag="VN647_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95623.1"
                     /translation="MYRTSQTRMTSTFAVILSMALLGSACTHAKSQSDRRVTGSPASQ
                     HQLQAQATKVDQKGDQQLLQGSQKVFGRVETVTSDQIKVDIGEMQPRFLPLKQAEEKN
                     FPQIKPGDDLVIIVNGQNLIVDYHPVDHPSGGHKIVRGAMADTMPVGHERVVIKSKDG
                     QKQSFEVRSQARSKLASIPVGTPMLFLIDETNKVTDATFASVDAAKEANKRPEDRSSI
                     KGANRQVGGTVVEPLSGDRITIKTGDGERPFEVREIMQERLSSLRKGESVILLMDNED
                     KVVDIAVPPASAR"
     gene            3305..4114
                     /gene="gntA"
                     /locus_tag="VN647_00970"
     CDS             3305..4114
                     /gene="gntA"
                     /locus_tag="VN647_00970"
                     /inference="COORDINATES: protein motif:HMM:NF041366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis heme-dependent
                     pre-guanitoxin N-hydroxylase GntA"
                     /protein_id="HWG95624.1"
                     /translation="MSVNMHTGMGNARRWNPLSIDPAASRFSNYAAFNGEAITRPFAP
                     EIPIDAELIDAHTRLRRQILSRFYPCTGAISAFSQKNYRFGLYPELACHSAVRAVCHD
                     LYCFCHEFSSIGDQFITFVAMFRGPAIRSERHFEDLLWNQLQGMHALDSDSFTWDKDV
                     ESDPASRHFSYSIGGRSMFVIGMHPEASRLARTRPYPTLVFNLHEQFERLRSRGKFET
                     MKQTIRAREMAFQGSINPMLSGFGENSEARQYSGRAVSPNWVCPFHKKAGA"
     gene            complement(4126..>5249)
                     /locus_tag="VN647_00975"
     CDS             complement(4126..>5249)
                     /locus_tag="VN647_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HWG95625.1"
                     /translation="ISQYHINADIMYALRKYVQATGDEEFLFDEGVEMLVETARLWLS
                     LGFYSLRQEGKFCIHCVTGPDEYSTMVNNNVYTNLMARENLRYAAETVEKVRECRPDL
                     FTALEHKTRLEASEVEDWKRAADLMYVPYDERLKITPQDDKFLEEEPWDFKHVPPEKY
                     PLLLYYHPLTIYRYQIIKQADLVLAMLLLGHEFSPELKRRNFEFYDPITTGDSSLSPC
                     IQSIMAAEVGDMTKALAYARVATLMDLGDVAGNVKDGCHIAAMGGVWMVFVYGLAGLR
                     DYTGRLSFRPALPEQFDCLRFRLAFQGQSLEIDVGQDATRYTLIAGNGLTLNHEDQEV
                     RLSPAAPSVVGPNRKLSKREGTNHVILTDGPWMEQQESS"
BASE COUNT         1185 a         1445 c         1439 g         1180 t
ORIGIN      
        1 agtagttcga gcagatggcc gttcggatcg cggaagtaga cgccgcgacc accattccaa
       61 tcattcaact tccggttttc tgcatcccac ggatgactgc cgtatgccaa tccggcctgc
      121 cgcactcggt cgaaaatcgc gtcgaattcg gtatcgtcca cgtgaaaggc atagtgatgg
      181 gactcaaacg aatcatcctg gtcgaaatcg agcgtgagtc tgtcattcac tcgtacggga
      241 gcgaaatgcc cggacgagcc ctcgtactga agcccgaaga tctcggcaaa aaatcgcgcc
      301 gacttgtcct tgtcatgcgc gggcacgatc gtatggttca gttcaatggt catggatgat
      361 ccctcgttct atgaatgccg ccagttcccg catgctctgg tcaaagcgtg ctttccagtc
      421 tgactgtgat gcaaaatgcg cgggattctc cacagactgc cgataaagtt tgagggtcga
      481 ttgcgcccat tcgccggcgg catctacgcc gtctcgaacg caaagcattg ttaaccgcat
      541 ttgttcgctc atcgtgctgt cgtagcaaga tcgagttagg gtagtatccg gccacttgtc
      601 ctccagtcaa acacgatcat tcggcgatcc aactcgcaga atccctagcg gccgagaggg
      661 tcggggaggt gttcttgcaa agcgacatca aaacaggtga tggagagaaa actacgtgaa
      721 gcggtgtaat tctatggaag tcatcgtgtg tggcctcggt gcagttccgg aaagaagcga
      781 atcatcagta atgagccttc ccggctgcct caatgatgac gcgcgtttca gtttgttcct
      841 cgggaaccgc cggtgtgccc gtatccgagt caccttgtcc ttcggtcgat cgagcccttt
      901 ccgccaaatg ctcgtataag gatcgaattt tatccaagtc ggtctgggac agcttttcca
      961 gatcgatcat cgcgttgtgc gctccctggg tcgagcgtat tatctcggcg agctttaatt
     1021 gaagggccgc gctatcgcga ttctgcgtgt tttgaatgag gaacaccatc aggaaggtca
     1081 cgatggtcgt gccggtattg ataacgagct gccaagtatc gctgaagttg aacagcggac
     1141 ccgtcaatgc ccagattgca acgaccccgc aagcgatcca gaatgctccg gcggtgccga
     1201 acatccgaga gcaggcgttc gcgactctgt tgaagacatt attatgttta ggtggaatca
     1261 cgaccgtcat tatttgcgga caggccgaac ataccaacta ggtagatccc ttaaaagcct
     1321 cgtagttcgg caatagaagg ctggacgttg ttcaattatt cgggcgtttt tgaggaaggt
     1381 cgcatcgaag gcttgtttca aaaagtcgat caaccgctcc gtctccatgc gggctcaatc
     1441 gccgacttca accacatgtc gtttcgcccg tgaaagccgg tcggaactaa atctccactg
     1501 tgctataccc cttcgccgag accgcgccca atcggtgcat gacctcgagc aggttcaact
     1561 cgtcccaatg ctcgacgaac ctgccgtcgc gcacgcgcca aatgtcgatg ctgcgcatcc
     1621 gtattgaacg gccggtcgcg gggaggccga gaaattcccc ctggtgcgta gcgcgatacg
     1681 tgaatcgacc gacgagcata tctccctcgg cgagcacgtc ctcgaccgtc acctcggtgt
     1741 cgggaaagga ccgcatccag ccctcgaaga actctttgat gccacggagt cccggtttca
     1801 catatggatt gtgattgatg taatcgtccg caatgaaatc gtcgaagcgg tctatctccc
     1861 ctgcgttgag cgcctcggcg tacttgtgag ccaactcctt aatactggtt gatgacatgt
     1921 ctcgtgctcc tttcagtcga gcaaggggct ccgtcggacg ggcgaggtgt tggcatgttt
     1981 ccatcatcgc ccgcggacct tcgtaattcg gcgacatgcc gtacttcttg cagcggtgag
     2041 acgaaaattt ttgctgagtg aaacagtttc tgagtgacga tacaactagg taaccctcta
     2101 gaactcatag gtaccgtcgg cggtcatccc ttaaataacc ggatgatttg tccttcctaa
     2161 aacaatttgt cctcataggg agccctagag ggttccctcc cggcatggca aactcgacgt
     2221 ggaaaactag agatgccgcg tcgacaacct gcacgcattt agccgcgtga agctcgcggc
     2281 acacgttggt cgcagcggcg tatcggcggt tgcaagagag tgctcatgaa taagtgggtt
     2341 aggcccatat cttgcgatca gttgactctg ttggtctccc aacgtgggct gcacaactaa
     2401 actcagcaaa aaggagagac tatgtaccgg acgagccaga cgagaatgac atctaccttt
     2461 gctgtcatcc tatccatggc gctgctggga agcgcctgca ctcacgccaa aagtcagagt
     2521 gatcgccgag ttaccgggtc gccggcctct cagcatcagc tccaggcgca ggcaacgaaa
     2581 gtggaccaga agggtgacca gcagctcttg caggggagtc aaaaggtctt cggcagggtg
     2641 gaaaccgtca catcggatca gattaaggtc gacatcggcg agatgcagcc ccgatttctt
     2701 cccttgaaac aggctgagga aaagaacttc cctcagatca agccgggtga tgatttggtc
     2761 attatcgtca acggccagaa tctgatcgtc gattatcatc cggtggatca tccgtccggc
     2821 ggccacaaaa tagtccgagg cgccatggca gacactatgc cggtagggca tgagcgggtg
     2881 gtcattaaga gcaaagacgg acagaaacaa tcgtttgagg tacggtctca ggcgcgaagc
     2941 aaactggcct ccatcccggt cgggacaccg atgctcttcc taatcgatga aacgaacaaa
     3001 gtaaccgacg cgacatttgc cagcgtagat gcagcgaagg aggcgaacaa gcgcccggaa
     3061 gacaggtcgt cgatcaaggg agccaaccgg caagtgggcg ggacggtcgt cgagccgttg
     3121 agtggcgatc gtatcactat taagaccggc gatggagaac ggccgttcga agttcgtgaa
     3181 atcatgcagg aacggctctc tagtcttcgc aagggagaat cagtcattct tctcatggac
     3241 aatgaggaca aagtggtgga cattgccgtc ccaccggcat cagctcggta attggattag
     3301 ccatatgtca gtgaatatgc acaccgggat gggcaatgcg cgccggtgga accccttatc
     3361 gatcgatccg gcggcaagcc ggttctcgaa ctatgcggct ttcaatgggg aggcaattac
     3421 gagaccgttc gcaccggaga taccgatcga cgccgaactc atcgatgcac atacgcggct
     3481 gcgacggcag atcttgagcc ggttctaccc gtgcaccggt gcaatctcgg ctttcagcca
     3541 aaaaaactat cgttttggac tttatcccga actggcttgc cacagcgccg tacgtgccgt
     3601 gtgtcatgac ctgtactgtt tttgtcatga gttctcgtcc atcggcgacc agttcattac
     3661 tttcgtcgcg atgttccgcg gaccggcaat ccggtcggag cggcacttcg aagatctgct
     3721 atggaatcaa cttcagggaa tgcatgctct ggattctgat tccttcacat gggataaaga
     3781 cgtggaatcg gaccctgcca gccggcactt ttcttacagc attggaggtc gatccatgtt
     3841 cgtcatcggg atgcaccccg aggcctcgcg tctggcgcgg acccgtccct atccgaccct
     3901 ggtgtttaac ttgcacgaac agtttgaacg tctgcgctcg cgcggaaagt tcgagacgat
     3961 gaaacagacg atacgggcgc gcgagatggc gttccagggt tcgatcaatc ccatgctgtc
     4021 cggctttggg gaaaactcgg aggcacgtca atattccggc cgggccgttt cgcctaattg
     4081 ggtatgcccc ttccacaaga aggccggagc atgatcactg caaaactagg atgactcctg
     4141 ctgttccatc caaggcccgt cggtgaggat cacgtggttc gttccctcgc gtttcgagag
     4201 tttccggttc ggtcctacaa cggacggagc agcgggcgag agccgaacct cttggtcttc
     4261 gtgattcaac gtcagtccgt tgccggctat caacgtatag cgagtggcat cttggccgac
     4321 atcgatctcg agactctgtc cttgaaacgc aagcctgaat cggagacagt cgaactgttc
     4381 aggcaaggcc ggccggaagc tcagtctacc ggtatagtcg cgcaatcccg caagcccata
     4441 gacgaacacc atccacactc cgcccatggc ggcaatatgg cagccatctt tgacatttcc
     4501 cgcgacatcg cccaaatcca tcaacgtcgc taccctcgca tacgccaggg ccttcgtcat
     4561 gtcgccgacc tcggcggcca tgatgctttg gatgcaaggt gagagtgacg agtctccggt
     4621 ggtgatgggg tcataaaatt cgaagtttcg ccgcttcaac tcgggggaga actcatggcc
     4681 gagcagcagc atggccagca cgagatcggc ctgtttgatg atctgatatc gatagatggt
     4741 gagcggatga tagtagagca gcagcggata cttctccggc ggcacgtgtt tgaagtccca
     4801 cggttcttct tccaaaaact tatcgtcttg cggagtgatc ttcagacgtt catcgtaagg
     4861 cacatacatg aggtcggcag cccgcttcca atcctccacc tccgaggcct ccagccgggt
     4921 tttgtgctcc agcgccgtga ataaatccgg ccggcattct cgcacctttt cgaccgtctc
     4981 ggcggcgtat cggagattct cgcgggccat cagattggta taaacattgt tattgaccat
     5041 cgtgctgtat tcatcgggac cggttacgca gtgaatgcag aattttcctt cctggcgcag
     5101 cgagtagaaa ccgagcgaca gccatagcct ggcggtttcg accaacatct ccaccccttc
     5161 atcgaaaaga aactcctcgt cgccggtcgc ctgcacgtac tttctcagcg cgtacataat
     5221 gtcggcattg atgtgatact gcgagatcg
//
LOCUS       DATNDD010000043         5904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3441849, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000043 DATNDD010000000
VERSION     DATNDD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3441849"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VN647_00980"
     CDS             <1..313
                     /locus_tag="VN647_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HWG95626.1"
                     /translation="KATDIAKSMVKAYGMSEKLGTITLERERQPQFLQLPVASEKGDY
                     SEETAREIDCEVRRIVDEQYGRVRRLLEDRKSALRQGAQLLLEREVMTGSELKAILEA
                     A"
     gene            411..1517
                     /locus_tag="VN647_00985"
     CDS             411..1517
                     /locus_tag="VN647_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG95627.1"
                     /translation="MEQATISIKDNVILCRGSWTLPHLSQLERGGRALRWPNTPAVLY
                     DAGEVTAMDTGGALILQRCIDGLRRNGQQTTLQGLKPEFAELLRKVEVQWSRPERAVS
                     VGTRWMDGLVRIVQSRQVSAIRALAFVGESTIAFSRALIRPHSIRWRTLLRIVDLDGV
                     RALPITGLLTFLVGVVIAYQGAEQLRKFGTNIFIVDLVGISLLREIAPLIVAILIAGR
                     SGSAYAAEIGTMKVTEELDAVRTLGISPMNLLVLPRALALVIALPLLTVYADAVGVFG
                     GMLIALGELNVSFVEFIARFEEAVPVRHFLIGLGKAPFFAAIIALVGCYQGFQIRGGV
                     DDVGRHTTISVVQGIFLVIVFDAICSILLNWWDL"
     gene            1632..2441
                     /locus_tag="VN647_00990"
     CDS             1632..2441
                     /locus_tag="VN647_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWG95628.1"
                     /translation="MISATETTTSIIEVSHVATKFGQAVVHEDVSLSIRRGEIFAIAG
                     GNGCGKTTLLREIIGLITPSAGRIRLFGIDSRQLETGDSHPIHRRFGVMFQHGALFSS
                     FTLAENVAVPLREHTTLSDGLIRDIVAAKIAMVGLPPDSAAKYPNELSGGMRRRAALA
                     RAIVMDPELLFLDEPTAGLDPIIAAGFDDLVLSLKDLLGLTVVMVTHDLDSLWRIANR
                     VAVLGRGKVLGIGTMQELSRSDDPVIREYFQGPRGRAASEQAAWNHERREA"
     gene            2576..3538
                     /locus_tag="VN647_00995"
     CDS             2576..3538
                     /locus_tag="VN647_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWG95629.1"
                     /translation="MEPKVNYILVGSFVAFLGAAVLVGILWLGKTDYRGAYDRYEAYM
                     RESVAGLSVDSTVKYRGVDVGRVKTIALNPNNPEEVLLTMEIMHGTPIKADTIAVLET
                     QGLTGLATINLTGGSRDAPSLQALEGHTYPIIKTGPSLLFRLDEAVSRLLSEEGLAQL
                     LVDLDTAAKGAAKVLDEDNRTMLKRTIKDLSDVTQTIAAHKTQIEQSLNGAARSADNL
                     VKLTAALNAEVPTLLAGINKSVAALGTATDELARTSKTVGAVVNEARPELQQFTRRTL
                     PEVGLLVTELRQLTGTLTRVAREVEREPSSLVFGRKAPSRGPGE"
     gene            3565..4203
                     /locus_tag="VN647_01000"
     CDS             3565..4203
                     /locus_tag="VN647_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWG95630.1"
                     /translation="MMNYRLAHVACALLVLTAAGCLSPRTDSSKIHTYQLSLDGWPSE
                     AGPGERDGPVLLVSPPQAEPGFETQRMVYVKRPYELEYYAVNQWADTPVRMFTPLMVQ
                     ALNQQNEAWRAVIPLPSSILGDYRLDTHGFLLQQEFLQQPSRVRVMVRAQLVELKGST
                     ILSTRAFEVVENAASENPYGGVQAANRAIAGLLDQIGSWLRQCVRHSPECSR"
     gene            complement(4253..5017)
                     /locus_tag="VN647_01005"
     CDS             complement(4253..5017)
                     /locus_tag="VN647_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HWG95631.1"
                     /translation="MLISFLSIERLADTSRQDHICLEPDSVHLWGIELDGSQRCLERC
                     SGWLDDVERHRAARLVRGDTRQRYMLTHGGLRAVLSRYLGVNPDVVALDRSATGKPFL
                     KNELRDRLPITFNLSHAHDRALIAISKGQEVGVDLEFVRTDIEVANLSQRFFTPSEHA
                     AIMQATEERRATRFFHYWVAKEALLKAQGIGLGGLPDCEIVFEADGVDTDVRARLGAR
                     FPNTLRVRLLPCEKGWEAAVAAQRLGAVKQCGLDSL"
     gene            complement(5075..>5904)
                     /locus_tag="VN647_01010"
     CDS             complement(5075..>5904)
                     /locus_tag="VN647_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWG95632.1"
                     /translation="VAMQPNGSAIPIFLVPGVMGNVLWLAQLARLLGQTHPVYGLQAR
                     GLDGDTAPFGSVPEMAQQYIAEIRTCVPQGPYVIVGACTGGLVAYEMAQQLAEQERAV
                     ILGIINSWHPSSYHQHYDTVRPSRLALPLGILLQTLRTVRELRRMPMSEWWSVIQRKC
                     KSFLSVFQGPTGNELRQRQIKRVRQAMFRAAARYTMRPYPGHILNIVASQRIESNDTR
                     YAWRELAGAGCRTIEMSALRTADLLVSPHVEDVSSHIRRYIVECSQDTPVRPNSRAA"
BASE COUNT         1269 a         1612 c         1779 g         1244 t
ORIGIN      
        1 caaggccacg gacatcgcaa aaagcatggt gaaggcctac ggaatgagcg agaaactcgg
       61 cacgatcacg ttggaacgag agcgacagcc tcaattcctt cagcttccgg tcgcttctga
      121 aaagggcgat tattccgaag agaccgcccg tgaaatcgat tgcgaagtgc ggcggatcgt
      181 tgacgagcag tacggacggg tgaggcgatt gctggaagat cggaaatctg ctctgcgaca
      241 aggagcccag ttactgttgg aacgagaagt aatgaccggt tcggagctca aggccatcct
      301 agaggcggcg tgaaccgagc aggacgacct tccttgcgat cgtcggcgat gattgtgcta
      361 gggtgtgctt gattacgtga agagcccatg tgtccgacgg agtgaatccg atggaacagg
      421 caaccatctc tatcaaagat aatgtgattc tctgccgagg gtcttggact cttccgcact
      481 tgtctcaact ggagcgagga ggccgagcgc ttcgatggcc taataccccc gccgtcctct
      541 acgatgccgg cgaagtgacc gctatggata cgggcggcgc gctcatactg cagcgctgca
      601 tcgacggcct gcgacgcaac ggacagcaga ccaccctcca agggttgaaa cctgaatttg
      661 ccgaattgct ccggaaagta gaagttcagt ggtcccggcc cgagcgagcg gtctcggtcg
      721 gtacgaggtg gatggacggt ctcgtgcgta tcgtgcagtc tcggcaagtg tccgcgattc
      781 gcgcgctcgc attcgtcgga gaaagcacca tcgcattcag ccgagcgctg atacgaccac
      841 attccattcg atggcggacg ctcttgcgga tcgtggacct ggacggcgta cgggccttgc
      901 cgatcaccgg tctgctgacg tttcttgtcg gcgtcgtcat tgcgtatcag ggcgctgaac
      961 agctccgtaa gttcggcacg aacattttta tcgtggatct ggtcggcatc tcgctgttgc
     1021 gcgaaatcgc cccattgatc gtggccatcc tcatcgccgg tcgttcggga tccgcctatg
     1081 cggctgagat cggcaccatg aaagtgacgg aagaattgga cgcagtgcgg acattgggca
     1141 tctcgcccat gaatctgctc gtgttgccac gagcgctggc tctggttatt gctctccctc
     1201 tgttaacggt gtacgcggac gcggtgggcg tgttcggcgg catgctgatt gccttgggag
     1261 agctcaacgt gagctttgta gaatttatcg ctcgattcga agaagccgtt ccggtgcggc
     1321 attttctcat cggcttaggg aaggcgccgt tcttcgcggc gatcatcgcg ttggtgggct
     1381 gctatcaggg atttcagatc agaggcggtg tggacgatgt gggccgacac accaccatca
     1441 gtgtcgtgca ggggattttt ttggtgatcg tgttcgacgc gatctgcagc atcctgctca
     1501 attggtggga cctttaagac gatgaagatg aataggaagg ctgaggctca ggttaaggtt
     1561 gaggccgaga ctacagtctc cggagattcc ttgaccttaa cctcagcctc aacctgcacg
     1621 agagcgtacc tatgatatcg gcgacagaaa ccacgacgtc gatcatcgaa gtcagccacg
     1681 tcgcgacgaa gtttgggcag gccgttgtgc atgaggacgt cagtctgtct atccggcggg
     1741 gtgaaatctt cgcgattgcg gggggcaacg ggtgcggcaa gaccacattg ttgcgggaga
     1801 tcatcggcct gatcacaccc tcggcgggaa ggatacggct gttcggaata gacagccgac
     1861 aattggaaac gggtgatagc catccaatcc atcgccgatt cggtgtgatg ttccagcatg
     1921 gcgcgctgtt tagttcgttt acgctggcgg agaatgtcgc cgtgcccttg cgcgaacata
     1981 cgacactgag cgacggattg atccgcgaca tcgttgctgc caaaattgcg atggtgggat
     2041 taccgccgga cagtgccgcg aaatacccca acgaactcag cggagggatg cgcagacggg
     2101 ccgcgctcgc ccgagcgatc gtcatggacc cggagttgtt atttctcgac gagccgaccg
     2161 ctgggctcga tccgatcatc gcggcaggat tcgatgatct cgtcctctcg ttgaaggatc
     2221 tccttggact gacggtggtg atggtgacac acgacctgga ttcgctgtgg cggatcgcca
     2281 atcgagtggc ggtgctcggt cgtggaaaag tgttgggcat cggcacgatg caggaattgt
     2341 ctcgatcgga cgaccctgtc atccgtgaat acttccaagg tccacgcgga agggcggcga
     2401 gcgaacaggc ggcatggaac cacgaaagac gtgaagcgtg aagcatcgaa taggagcaga
     2461 tggcttatgg ctgataacac gaacgaggga tacgaactca ttgtccggct tcgccttttt
     2521 gctataagcc atacgctata agctattcgc tcttctgggc gagattaagt taaatatgga
     2581 acccaaggtc aactatattc tggtcggctc gttcgtggcg tttcttggcg cggccgtctt
     2641 ggttggaatt ttgtggctcg ggaagaccga ctaccgaggc gcttatgatc ggtacgaagc
     2701 ctacatgagg gagtcggtcg cgggattgag cgtcgattcc accgtcaaat atcgaggtgt
     2761 cgacgtgggt cgggtcaaga ccattgcgtt gaatcccaat aacccggagg aagttctgct
     2821 gaccatggag attatgcatg ggacgccgat caaggccgac acgattgccg tactcgagac
     2881 gcaggggctc accggtctag ccaccatcaa tttgacggga ggaagccgag atgctccctc
     2941 gctgcaggcc ctggagggac acacatatcc gatcatcaag accggtccct ctctcctctt
     3001 ccgcttggat gaagccgtct cccgattact ttcagaagaa ggcctggctc aactattggt
     3061 cgatctcgat acggcggcaa aaggagcggc gaaggttctg gacgaagaca atcgcaccat
     3121 gctgaagcga acgatcaagg atctttcgga tgtgacccag accattgcgg cccacaagac
     3181 tcagatcgag caaagcttga acggtgcggc caggagcgcc gacaatcttg tcaagctgac
     3241 ggcggcgttg aacgcggagg ttccgacctt actcgccgga atcaacaaaa gcgttgcggc
     3301 gctgggcacg gcgacggacg aactggctcg cacgagcaag accgttggag cggtcgtcaa
     3361 tgaagccaga cccgaactgc aacagttcac cagacggact cttcccgagg tcggcttgtt
     3421 ggtgacggag cttcgacagc tcacgggtac attgactcgt gtggcgcgcg aagtggagcg
     3481 agagccgagc tcgctggtgt ttgggcggaa ggccccgtca cgcggaccgg gggaataaca
     3541 gcaaggtgtg tgtacggtca tgacatgatg aactatcgac tggcacatgt ggcctgtgcg
     3601 ctgctggtgt tgacggcagc cggttgcctg tcgccccgca cggattcttc gaagatccat
     3661 acgtatcaat tgagtcttga cgggtggccc agcgaggcgg ggcccggcga gcgtgacggt
     3721 cctgtactgc tcgtgagtcc gcctcaagcc gaacccggat tcgaaacgca gcgcatggtg
     3781 tatgtcaagc ggccgtacga actggagtac tatgcggtga atcaatgggc cgatacgccg
     3841 gtgcgcatgt tcaccccgtt gatggtccag gcgctcaatc agcagaatga ggcatggcgt
     3901 gccgtgattc cactgccgag ctcgattctc ggagactatc gtcttgacac ccacggattt
     3961 ctgttacagc aagaatttct tcaacaaccc agtcgcgtgc gggtaatggt tcgggcgcag
     4021 ctcgtcgagt tgaaagggtc gacgatcctg agcacgcggg cttttgaggt cgtggagaat
     4081 gccgccagcg agaatcccta tggaggtgtc caggctgcca accgagcgat tgccgggttg
     4141 ctcgatcaaa tcggctcttg gcttcggcaa tgcgtccggc attcgcctga gtgcagtcgc
     4201 tgatggggcc ggtgcaggat ccggtggaaa acttgaactc aacagagcgc gattacaggc
     4261 tgtcgagtcc gcactgttta accgcgccca gcctctgagc agccaccgct gcttcccatc
     4321 ccttttcgca agggagaagc cgaacccgca acgtatttgg gaaccgagca cccaatcggg
     4381 cccgaacgtc tgtgtcaacc ccgtccgcct caaaaacaat ctcgcagtcg ggcagtcccc
     4441 cgaggccgat accttgcgcc ttgagcagcg cctctttggc gacccagtag tggaagaatc
     4501 tcgtggcgcg ccgttcctcc gtcgcctgca tgatcgccgc atgttcggag ggagtaaaaa
     4561 agcgctggga tagattcgca acctcgatat ccgtacgaac gaattcaaga tcgactccga
     4621 cctcctgccc ttttgagatt gcaatgagcg cacggtcatg ggcatgggac agattgaacg
     4681 tgatcggcaa ccgatctcgc agttcatttt ttaggaacgg tttgcctgtc gcactgcgat
     4741 ccagcgcgac cacatccggg ttaacgccga gatacctact cagcaccgcc ctaaggcctc
     4801 cgtgagtcag catgtagcgc tgccgtgtgt ctccacgaac aaggcgtgcc gctcgatgcc
     4861 gctcgacgtc atccagccac ccgctgcatc gctccagaca gcgttgcgat ccgtcaagct
     4921 cgatccccca caggtgaacg gagtccggtt caagacagat atgatcctgt cggcttgtat
     4981 cggcgaggcg ctcaatggac aggaaggaaa tcagcacatt gttaccgtga atgccgaatc
     5041 gccttcgttg ttcggcaaag gaaggtggtg aaaatcacgc agctctacta ttcggccgaa
     5101 cgggtgtatc ttgagaacat tcgacgatat agcgccgaat gtgggaggat acgtcttcga
     5161 cgtgcggcga aacaagcagg tctgccgttc gaagggccga catctcgatg gtccggcatc
     5221 ctgcgcctgc cagctctctc caagcatatc tggtatcgtt cgactcaatt cgttgcgaag
     5281 ccacgatgtt gagtatgtgt ccgggatagg ggcgcatcgt atagcgggcg gccgcacgga
     5341 acatcgcctg tcggacgcgt ttgatctgac gctgtctcag ctcattcccg gtaggaccct
     5401 ggaaaacgga caaaaaagat ttgcatttgc gctgtatgac cgaccaccat tcgctcatgg
     5461 gcatgcgccg gagctcgcgg acggtgcgta aggtctgcaa gagaattccc aacggcaatg
     5521 caagccggct tgggcggacg gtatcatagt gctgatgata cgaactcgga tgccagctat
     5581 ttataatgcc tagaatgacc gctctctcct gctccgccaa ttgttgagcc atctcatagg
     5641 caaccagccc accggtacat gctcccacga tcacataggg tccctgaggg acgcatgtgc
     5701 ggatctcggc gatatactgc tgcgccatct ccggcactga accgaatggt gcggtgtccc
     5761 catccaatcc tcgcgcttgt aagccgtaca ccgggtgagt ttgtcctaag agcctcgcca
     5821 actgagcgag ccataacaca ttgcccatta ctccgggaac caagaaaata gggatggccg
     5881 acccgtttgg ctgcatggcc acca
//
LOCUS       DATNDD010000044         3233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2838968, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000044 DATNDD010000000
VERSION     DATNDD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2838968"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VN647_01015"
     CDS             complement(<1..1331)
                     /locus_tag="VN647_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG95633.1"
                     /translation="MASLGRGVEGSGERALVKRGVGLEAITIEQGPTLAKLPCWEAVQ
                     IPGGMFTASRAILTILLLFLIVLEFVPWTQTISVNGKVSAYTPYDRPQKIESRITGRV
                     KAWHIYEGVKVKKGDLVGELEDYDPTFMAPEILPLFEQRKVALEQTRQAALARADQLN
                     KRIGEMKKLVQAAVPSAEARVVEADNKVREAQQKVEQLKIDVHTAQLNVDRHRQLVRD
                     GLVSQRELELTIQTEIGTKAGLQAAQASLLAAEQARSALSFGRDQITADVQQRLIDAI
                     ASRDAAVAEAAKATEQLADISYRQQGVQQRIEAARLIAPMDGTVVKMAKVGINETVKQ
                     GDNLVTISPLATDPAIEMVAEGLDSPLLKPGRKVRILFFGVPAIPLPAWPSLMAGTRG
                     GVIKVVDQIDDGKGNYRFWVVPDPDDPQPWPEQAQVRQGTKVLGWVIMNRVP"
     gene            complement(1377..3053)
                     /locus_tag="VN647_01020"
     CDS             complement(1377..3053)
                     /locus_tag="VN647_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWG95634.1"
                     /translation="MKEPQGEGNQGLFEALMKQLSVAMRAEKKIMSLIFSYALAVGFF
                     SLIIPLTVQELVSTFSYAVQPVMIWTLTGIMLAVLLFVGLFKTFHFYAVDVVQRRLFA
                     RVAVAMVEQLPRNRFKGVQSELANYFIETVFMQRALSTLLIDLMNVVVGGTVGMIVLI
                     VYHPYFLVYNLLLMAGFGITFFVLSRGALKTTLAMSHAKYDVFNWIQEISRNALQLKA
                     TDSRPFLIQKTNDLVAHYVETRKARFAVLVRQYIGSVGGQAIAQSGALALSAWLMASG
                     QLTLGQLVAVQAVVGALVINFDSLIKNMGYVYYFFTSLTHLDTIFRQEQDYVSVESPV
                     GLPKHLTQGVRVTCKGVSLIHGGVSVFDGFNLDVAPGEKLGIYARTTGAKTALARVLG
                     GLETPTNGVVQYNGVDLRYIDPNAINQTRSVMIDSQLSLIKGTIEENIVMGRSYVTYD
                     DLNWALRFTELEEDVEGLARGVKSDVSSLGESLSPTHVVQILLARAILGRPQVLIFDG
                     LIHSLEPSLRERVLRRLCSKDEAWSVIFVSTDPNLTDHADRRIMLHHAHA"
BASE COUNT          803 a          919 c          782 g          729 t
ORIGIN      
        1 gggacacggt tcatgatcac ccaacccaat acctttgtgc cctgtctcac ctgagcctgc
       61 tcaggccatg gttgaggatc atccggatca ggaacaaccc agaaccgata atttcctttt
      121 ccatcgtcga tctggtcaac caccttgatg acgccccccc gagtacccgc catcaacgaa
      181 ggccaggcag gcaacgggat agccggcact ccaaaaaaca agatccgaac cttccgtcca
      241 ggtttcaaaa gtggagaatc caacccttcg gccaccatct cgatggctgg atcggtggcg
      301 agcggagaaa tggtgaccag attgtctccc tgtttaaccg tctcattgat accgaccttc
      361 gccatcttga cgacggttcc atccatcgga gcaatgagac gcgcagcctc gatccgttgt
      421 tggacgccct gttggcggta cgagatatcc gccaactgtt ccgttgcctt ggcggcttca
      481 gccacagcgg catcgcgcga tgctattgca tctataagcc gttgctgcac atcggctgtg
      541 atttgatcac gtccgaagct caaggcggat cgagcctgct cggcagccaa taaacttgct
      601 tgcgctgcct gcaaacctgc ttttgtgccg atttccgtct gaatcgttaa ctcgagttcc
      661 cgttgtgaaa ccagtccgtc tctgacaagt tgccgatgcc ggtcgacatt gagttgcgct
      721 gtatgtacat cgatcttaag ctgttcgacc ttctgttgcg cctctcgcac tttgttgtcg
      781 gcttccacca ctctggcttc agccgacggc acggcggcct ggaccagctt tttcatctcc
      841 ccaatcctct tatttaattg atccgcccga gctaatgctg cctggcgcgt ttgttccaga
      901 gctaccttac gttgttcgaa aagtggaagg atctcgggcg ccatgaaggt cggatcatag
      961 tcttcgagct cgccgacgag gtcgcctttt ttgaccttaa ctccttcata aatatgccat
     1021 gccttgacac gacccgtaat ccgagactcg atcttctgcg gccggtcgta gggagtataa
     1081 gcggaaactt tcccattcac agaaatggtc tgcgtccagg ggacaaactc aaggacgatc
     1141 agaaaaagca ggagaattgt cagtatcgcc cgagacgccg tgaacattcc acccggaatc
     1201 tgcaccgctt cccagcacgg caactttgcc aatgtgggtc cctgttcgat agtgatcgct
     1261 tccaagccca cccctcgctt gaccagcgca cgttctccgc tgccttcgac accgcgacct
     1321 aggctagcca tggagcctcc attaatttac cggtgttgac ttgccattcc aacagtttac
     1381 gcatgagcat gatgcaacat aatacgacga tcagcatgat ccgtaagatt tggatccgtc
     1441 gacacgaaga tcacagacca tgcttcatct ttagagcaca accgacgcag gactcgctcg
     1501 cgcaatgatg gctcgagcga gtgaatcaat ccatcaaata tgagaacttg tggacgtcct
     1561 aaaatcgctc gggccaaaag aatctgcaca acgtgagtcg gagaaaggga ttctcccagc
     1621 gaggagacat cggacttaac accgcgcgcc aaaccctcga catcttcttc aagttcggtg
     1681 aaacggagag cccaattgag gtcatcatag gtgacatagg aacgccccat aacgatattt
     1741 tcttcgatcg ttcccttgat taaggacaac tgagaatcga tcatgacgct ccgggtttga
     1801 ttgatcgcat tgggatcgat ataccgaaga tccacgccat tgtactgaac aacgccattg
     1861 gtcggagttt cgagcccgcc caacaccctg gccaaagcgg ttttggcccc agttgtccgc
     1921 gcgtagatac caagcttctc gcctggcgcc acatccaggt taaagccatc gaacacagac
     1981 actccgccgt gaatcaagct gacacctttg caggtaacac gcaccccttg agtcaaatgt
     2041 ttgggaagtc ccacgggcga ttcgaccgag acataatctt gctcctgcct aaagatcgta
     2101 tccaggtgtg tgagggacgt gaagaaatag tatacatatc ccatgttttt tatgagggag
     2161 tcgaaattga tgacaagcgc gccgacgacc gcttgaacag cgaccaactg cccgagcgtc
     2221 aactgccccg atgccatgag ccaggctgag agagcaagag ctcctgattg tgcgatcgct
     2281 tgtcccccca ctgagccaat atattgccgc acgagaacgg caaatcgggc tttccgagtt
     2341 tcaacatagt gagcgacgag atcatttgtc ttctggatga gaaatgggcg gctgtcggtg
     2401 gctttcaact gcaacgcatt ccgcgatatt tcttgaatcc aattgaagac gtcatatttg
     2461 gcatgagaca tggcgagggt cgtcttcagt gcaccacgag aaagtacgaa aaacgtgatg
     2521 ccaaaccccg ccatgagcag taggttgtac accagaaaat agggatggta gacgatcagc
     2581 acgatcatac cgactgtccc acccaccacg acgttcatca ggtcgatgag cagcgtggat
     2641 aaggcccgtt gcataaagac cgtttcgatg aagtaattcg caagctcgga ctgcacccct
     2701 ttaaatcgat tccggggaag ctgttcaacc atcgcaaccg cgactctcgc aaagagtcgg
     2761 cgttgcacga cgtccaccgc atagaagtgg aatgtcttga acagaccgac aaacaacaga
     2821 accgccagca tgatgccggt tagagtccaa atcatgaccg gctgaaccgc ataggaaaag
     2881 gtgctcacca attcctgcac ggtcaacggg atgataagag aaaagaaccc aacggccagg
     2941 gcataagaaa aaatcagact catgattttt ttctcagccc gcatcgccac ggaaagctgc
     3001 ttcatcagcg cttcgaacag gccctgatta ccttcgccct gaggttcttt catgagcacc
     3061 cccagtaata agtcagaatg gattgatcgg ccccttgctg aacttaatga cggctgaact
     3121 aaatcacgtt tgtgaatcta cttccaagcc catcgaatga actatacggt agtaccaccc
     3181 ttcttcctga catcagtcgc gtcccggtag tttacgaccg gacgagccgg ctg
//
LOCUS       DATNDD010000045         4494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_116920, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000045 DATNDD010000000
VERSION     DATNDD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116920"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VN647_01025"
     CDS             complement(<1..591)
                     /locus_tag="VN647_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWG95635.1"
                     /translation="MRYWPTFLMLVILAGLGGYLYLIEFPAEQREEKQESAQKQILPF
                     PETAITALSITTPQGPVAFKLAEPGKWSIVAPLQTDADNREVQALIRALVTGVVARTV
                     EEQATQLAPFGLEKPVTTLTITAGTQQETISIGDSGPLSNTLYVLRASDRRVLLTNLA
                     PKDFINKSLMTFRRKELLRFVQNDVERVRLTYPKTEI"
     gene            complement(694..2217)
                     /locus_tag="VN647_01030"
     CDS             complement(694..2217)
                     /locus_tag="VN647_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWG95636.1"
                     /translation="MNLKSFPLGILGVVLGIGGMITYSLRPDWLWVATIAEGLALVCL
                     VLFFILHFETVKAFSSRRSTRMGLNSFLMIVLFSGILVIVNFLASRHSVRWDLSENQN
                     FTLAPQTHRVLRTLPHDVKITVFTREKDPGYQPYKERLESYRQASTKLSVEFIDPEKQ
                     PKIAQSYGIFRTDTAIFESNGQTIRVTSPSEVELTGALIRISKDAKKRIVFVEGHSEL
                     SMEDKDRNGLSIAKESLTRQGYDVGTLSLLKESAVPENTSVLVLAGPRRSVTKEEQER
                     IQAYVEKGGHLLVLADPDTQTGLEPMLAHWGLGLGPGVLVDLQDRLAQGDLTALLVRT
                     FTEHEITQDLTSAVLLPLSRHVTFDEQVGKDWDFVPLARTSPNSWAETNMQGRVVSLS
                     EKEDVKGPLPMAAALAPKKAPEEGKPRPAVVVIGNSTFVSNAFFNFPGNSDFFLHTIG
                     WLAEERDLISIAPKEPALHPFTPNPTQERALIYIQVVFLPLVTLLTGIVVWRKRRRL"
     gene            complement(2214..2984)
                     /locus_tag="VN647_01035"
     CDS             complement(2214..2984)
                     /locus_tag="VN647_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWG95637.1"
                     /translation="MTPVQAVIAKELRGYFVSPIVYVVGAVFLLIFGLLSYLYVGFAG
                     AQAIQLMQMQGGMAQINLNDLVFRNLFASMRIVLVIILPILTMRLFAEERKLRTFEFL
                     MTSPIRINEIVVGKFVSVFLIYLGLVGLTTLVPTVLMLFSDFDWNPIWTGYLGMVLLG
                     ALFLAVGVFASALTENQIVAAFVSFGMLLTIWLIAGLGSLFGDTTAGRVISYFSYMEH
                     YDRLVRGLIDTSDLIYFGSGLALMLFLTHRVVESTRWK"
     gene            complement(2992..4068)
                     /locus_tag="VN647_01040"
     CDS             complement(2992..4068)
                     /locus_tag="VN647_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWG95638.1"
                     /translation="MSKIHDQTTWCNFESGAAPFFSTVIRIDITSVIDVQNITKRYGH
                     HTAIDRVTFSVAKGEVLAFLGPNGAGKTTTMRILTCFMPATEGTAGVAGFDCTEQPLE
                     VKRQIGYLPETPPVYQELTVAEYLTFVGRLRGITGKKLSSALDQSIGRLELGSVRHRL
                     IGNLSRGYRQRVGLAQALLHDPPVLILDEPTVGLDPKQIIEIRELIKTLAGSHSVILS
                     THILPEATAVCQRVVIINKGRIVAEDTPEQLSARLRQSEKVSITLKTCPSDCEMKLRA
                     IHGILNVLPGQVGGSFLIECELGRDLRDEIARVAVMSQWGLLELRTVSMTLEDVFLQL
                     TRHESHLSEPVEATVSASAERTDE"
BASE COUNT          994 a         1222 c         1298 g          980 t
ORIGIN      
        1 gatttcggtc ttcggatagg tcaggcggac ccgttcgaca tcattctgga caaaccgcaa
       61 gagctctttg cggcggaatg tcatcaacga cttgttgata aagtccttcg gcgccaagtt
      121 ggtcagcaag acgcgccggt ccgatgcgcg aagtacatag agggtattgg aaagaggacc
      181 gctgtcgccg atggaaatcg tctcttgctg cgttccggct gtgatggtga gggtggtcac
      241 cggcttctca agaccgaacg gcgcgagctg cgtggcctgt tcctcgacgg ttctggcgac
      301 gactcctgtc acgagcgctc gaataagcgc ctgcacctct cgattatcgg cgtcggtttg
      361 aagcggagcc acgatagacc atttacccgg ttccgccagc ttaaatgcga cgggtccctg
      421 aggggtggtg atcgagagtg cggtaatcgc ggtttccgga aaaggaagga tctgtttctg
      481 ggcgctttcc tgtttctcct ctcgctgctc ggcgggaaac tctataaggt agagatagcc
      541 gcccagacca gcgaggatca ccaacattag aaatgtaggc cagtagcgca tcaagtctct
      601 tgaaggttaa ggctgaggtc gagtggaagt ggttgaatca atgaccttaa ccgcaatctc
      661 aaccttaacc tcaacctttt tctctggtgc ccgttacagg cggcggcgtt ttctccacac
      721 gacgatgccg gtgagcaacg tcacgagcgg aaggaacacg acctgtatgt aaatcagtgc
      781 ccgttcctgg gtggggttgg gtgtgaacgg gtgcaatgcc ggctctttcg gtgcgatgga
      841 aatcaagtcc cgctcctcgg ccagccaacc gatcgtatgg agaaagaagt cgctattgcc
      901 ggggaagttg aaaaaggcgt tggacacgaa ggtcgagttc ccgatcacca cgacggcggg
      961 tcgaggtttg ccctcttccg gggccttctt cggcgccagg gcggcagcca tgggaagcgg
     1021 tcctttcacg tcctcctttt cactcagact cacaacccgg ccctgcatgt ttgtttcagc
     1081 ccagctgttc ggcgaggtac gagccagcgg cacaaagtcc cagtctttcc cgacctgctc
     1141 atcgaacgtg acgtgtcgcg aaagcggcaa gagcacggcc gaggtgaggt cctgcgtgat
     1201 ctcatgctcg gtgaacgtcc gaaccagcag cgcggtgagg tctccctgcg ccaatcgatc
     1261 ctgtaaatcg accaacactc ccgggcctag gccaagtccc caatgggcca gcattggctc
     1321 caggcctgtc tgagtgtcgg gatcggccaa gaccaggaga tggccgccct tttcgacata
     1381 agcctgaata cgttcctgtt cttccttcgt tacggaacgg cgaggtccgg ccaacaccag
     1441 caccgacgta ttctccggca cagcggattc ttttaacagc gagagggtgc cgacgtcata
     1501 accttgccgg gtcagggatt ctttggcgat ggagaggccg ttgcggtctt tgtcttccat
     1561 actcaactcg ctgtggcctt cgacgaagac gatgcgcttc ttggcgtcct tggaaatgcg
     1621 aatcagggcg ccggtcagct ccacttccga tggtgaagtc acgcggatcg tctggccatt
     1681 gctctcgaaa atcgcggtat cggttcggaa gatcccatag ctttgggcga tcttcggctg
     1741 tttttccggg tcgatgaatt cgacgctgag cttggtggag gcttgccgat aactctccag
     1801 tcgttccttg tatggttgat agccgggatc cttttccctg gtgaagaccg tgatcttcac
     1861 gtcgtgcggc aaggttcgta gcacccgatg ggtttgcggg gcgagggtga agttctgatt
     1921 ttccgacaga tcccaccgta ccgaatgccg cgacgcgaga aaattcacga tgaccaaaat
     1981 gccggaaaag agcacgatca tgagaaagct gttcagcccc atcctggtcg accgtcggct
     2041 ggagaatgcc ttgacggtct caaaatgcag aatgaagaac agcaccaaac agaccagtgc
     2101 cagcccctcc gcgatcgtag cgacccacag ccagtcaggt cgaaggctgt acgtgatcat
     2161 gccgccgatg ccgagcacga ctccgagaat gccgagagga aaggatttca ggttcatttc
     2221 cagcgagtcg actctacgac gcgatgagtg aggaacagca tgagcgccaa tccgcttccg
     2281 aagtagatga ggtcgctcgt gtcgatcagt ccccgcacga ggcggtcata gtgttccatg
     2341 tatgaaaagt atgagatgac gcggcctgca gtggtatctc cgaacaggct tcccaatccg
     2401 gcgatcagcc agatggtcag gagcatgccg aaactgacga aggcggcgac gatttgattc
     2461 tccgtcagcg ccgaggcaaa cacgcccacg gcaagaaaca gggctcccag cagcaccatt
     2521 cctaaatagc cggtccagat gggattccaa tcgaagtcgc tgaagagcat cagcaccgtc
     2581 gggaccaatg tggttagtcc tacgaggccg agatagatca agaacacact gacgaatttg
     2641 ccgacgacga tttcattgat cctgatcggc gaggtcatca agaactcaaa tgtgcggagt
     2701 ttgcgttctt ccgcgaacaa ccgcatggtc aagatgggca gaataatcac gaggacgatc
     2761 cgcatgctcg cgaagagatt ccggaaaacc agatcgttca agttgatctg ggccatgcct
     2821 ccttgcatct gcatcaattg gatggcttgc gcgccggcga aaccgacgta aagataggag
     2881 agtagcccga agatcagcag aaagaccgcg ccgacgacat agacaatggg agagacgaaa
     2941 tagccgcgca gttccttggc gatgacggct tgcaccgggg tcataaggct cctactcgtc
     3001 cgtcctttcg gcggaggcac tgaccgtggc ttccaccggc tcggaaaggt ggctctcgtg
     3061 gcgcgtgagc tggaggaaga cgtcttccag cgtcatagat acggtgcgga gctcaagcaa
     3121 cccccactgg ctcataacgg ccacgcgtgc gatttcgtcc cgtagatcgc ggcccagctc
     3181 gcactcgatg agaaagcttc cccccacctg cccaggcaaa acattgagaa tgccgtgaat
     3241 cgcgcgaagc ttcatctcac agtccgatgg gcatgtcttg agggtaatcg agaccttctc
     3301 cgattgacgt aatcgggccg acagctgttc aggggtatct tcggcgacga tgcgaccttt
     3361 attgatgatg accacccgtt ggcagactgc ggtggcctcc gggagaatat gcgtgctgag
     3421 aatcacggaa tgcgagccgg ccagagtctt gatgagttct cggatttcaa tgatctgctt
     3481 gggatcaagg ccgaccgtcg gctcgtcgag gatcagcacc ggggggtcat gcagtagcgc
     3541 ttgcgccagc cccacacgct gacgatagcc gcgggaaagg ttgccgatca gtcgatggcg
     3601 gacggagccc agctcaagtc gtccgatcga ttggtcgagc gccgacgaaa gtttcttgcc
     3661 tgtgattcca cggagtcgac cgacaaaggt cagatattcc gccaccgtga gttcttggta
     3721 gaccggagga gtttccggta ggtatccgat ttgccgtttc acctccaatg gctgctcggt
     3781 gcaatcaaat cccgccactc ctgccgtgcc ttctgtcgcc ggcatgaagc aggtcaagat
     3841 ccgcatggtg gttgttttgc ctgcgccgtt gggcccaagg aatgccaaca cctctccctt
     3901 ggccaccgag aaggtgacgc gatcgatagc cgtatgatgg ccataccgct tggtaatgtt
     3961 ttgaacgtcg atcacggatg tgatatcgat acgaataact gtggagaaaa atggagcggc
     4021 accgctctcg aaattacacc atgttgtttg atcatggatc ttactcattc cgtccgagtc
     4081 agtcaatata gatagtaggg tggggtatca agcatgatgg atcgtctaca cagtatcagg
     4141 gtcgtgacgg ctcgaaggtg actcttcgtt actcggccgg atagcatcat ctccgtgaag
     4201 atacgcattt cgaagatgtc ctgtcttcgt tgcaggttcc gctcgacccg ctggtattga
     4261 atttaagtcc cggttcattt atatagaggt cgcgaggaag cctatccttt acatttccat
     4321 gcggtcactc tctccattac ggaacggaag actccatacg gaggtggggg gaccggtaca
     4381 ccagtcggta ttgtcgaagc cccatacctt tcatactctg aatcgaccag tgatctcatt
     4441 gttgacatca gaggaggcga tacccagcat gtacactgag cgtcatcgca gtcg
//
LOCUS       DATNDD010000046         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_116839, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000046 DATNDD010000000
VERSION     DATNDD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116839"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VN647_01045"
     CDS             complement(<1..842)
                     /locus_tag="VN647_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWG95639.1"
                     /translation="MSDSTLSPLMRQYRDIKQGYPEAILFFRVGDFYEMFYEDAQDAS
                     RLLSIALTSRDKNSAHPVPLCGVPYHAATGYIAKLLKAGRIVALCEQVEDPKAAKGLV
                     RREVVRLYTPGTLVDTEFLAPSELNYLAALAVQSGTIHGLPIGLAVLEVSTGEFWGME
                     FHGPHAFTYILNEVARLDPREVLFPAGLVHQSSAWIQQITGARLCERPPASFSRQSAV
                     HTLTEHFHVQTLEALGCADFPAASSAAGAVLSYFRETQPTVPLDHVRRFIVRHTNEYM
                     HLDGA"
     gene            complement(835..1179)
                     /locus_tag="VN647_01050"
     CDS             complement(835..1179)
                     /locus_tag="VN647_01050"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HWG95640.1"
                     /translation="MTRLILVGTLLLLSLAFFLQNQEQEVTLRYFFGLLEASTPIYKP
                     IMAGFMIGLLVAAILLFPPWIRGRIDLRRKTKALQEAEVNLERLRQSLEKMSGRTQGP
                     AQMESSRDLADE"
     gene            complement(1176..1718)
                     /gene="tsaE"
                     /locus_tag="VN647_01055"
     CDS             complement(1176..1718)
                     /gene="tsaE"
                     /locus_tag="VN647_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWG95641.1"
                     /translation="MATSISSLDFILSSPGETESFGHAIGRLLRGGEVLALIGELGAG
                     KTALVRGIVAGLGAPAASVTSPTFLFVHEYQGRLPVVHIDLYRLQRLEEIELIGLSDY
                     LTDEVVVAIEWADRFPQWLPDDRLEVQVAHRTRTTRKAHVEARGPRSRLLLAQIKKIV
                     YPIPRSVRPRLRGGRRTSPR"
     gene            complement(1729..3327)
                     /locus_tag="VN647_01060"
     CDS             complement(1729..3327)
                     /locus_tag="VN647_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWG95642.1"
                     /translation="MTKIITAAQMQELDRRTISEAHIPATTLMERAGSGVVACLEQRL
                     GSVRGKTVTVVCGKGNNGGDGFVAARLFRRRHAKVRVLAMAPTSELSRDAAAMYRQFV
                     RGAGKSSVYLYTSRIQAQALLHDSDIIIDALLGTGLSSTVTGRYAEAIDGINETSRPV
                     VAVDLPSGLHADTGAILGRAVRASLTVTFGLPKLGLYQNYGIDLAGEVAVVDIGIPPT
                     YLDAVESRTTLMTQHVVRAYLPKRQPSSHKGTFGHAGIIAGSVGKTGAAAMAARAALR
                     IGAGLVTVAVPTSINDVLESKLLEVMTVPMPETKARTFARTALDRLVAFMSARTAIAI
                     GPGLSTHPETVELVQALTKLLDRPAVLDADALNALTGRTALLASCKTPPIITPHPGEM
                     ARLEADATPQTVNSDRIGFASRFARERGLFVVLKGARTVVARPDGAVAVCPTGNPGMA
                     TAGTGDVLTGMMVGLLAQGIPSWEAACAATYLHGTAGDLAAVRTGQAGLIAGDVIEEI
                     PHALRLIHQASSKDKPIEIWNALL"
     gene            complement(3379..>3834)
                     /locus_tag="VN647_01065"
     CDS             complement(3379..>3834)
                     /locus_tag="VN647_01065"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWG95643.1"
                     /translation="DLVTLVPEKRQELTTEGGLDIVGQRDRIQNIVTLLHNGGIPVSV
                     FVEPDLSQIRAAHKIAVDFVELHTGRYANATRSKEADAEFEAITHAAKLAYKLGIGVN
                     AGHGLNYRNIKRLTHIPEIVEYNIGHSIIARAVLVGLVQAVKEMKTLLG"
BASE COUNT          845 a         1070 c         1115 g          804 t
ORIGIN      
        1 gctccatcca ggtgcatgta ctcgttagta tgccggacga tgaatcggcg aacgtgatcc
       61 aaaggaaccg tcggttgggt ctcgcggaag tacgacaaga cagctcctgc agcactactg
      121 gcggcaggga agtcggcaca gcctaaggct tccaaggttt ggacatggaa gtgctcggtc
      181 aaggtgtgaa cagccgattg tcggctgaac gacgccggtg gtcgttcaca caggcgtgct
      241 cccgtgattt gttgaatcca agcggaggat tggtggacaa ggcctgcagg gaagagaact
      301 tcacgaggat ccaaacgagc cacttcgttc aatatgtatg tgaacgcgtg aggaccgtga
      361 aactccatac cccaaaattc gcccgttgaa acctcaagga cggccaggcc tatcggtaga
      421 ccatgaattg tgcctgattg aacggcgagc gcggcgagat agttgagctc ggaaggggca
      481 agaaattccg tgtctaccag tgtgccggga gtataaagtc tgacgacttc tcggcgtacc
      541 aggccttttg ctgccttggg atcctcaact tgctcgcaca acgcgacaat acggccggcc
      601 ttcaataatt ttgcgatata gcctgtggcc gcatgatagg gaacgccaca cagtggaaca
      661 ggatgggcgc tgttcttgtc acgggaagtg agggcgatcg ataagagtcg agacgcgtct
      721 tgtgcgtcct cgtagaacat ctcataaaaa tctccgaccc gaaaaaacag tatggcctca
      781 gggtatccct gtttgatgtc ccgatactgc cgcatcagcg ggctcagggt cgagtcactc
      841 atcagcgagg tccctcgaag actccatttg agcgggaccc tgagtccgtc cggacatctt
      901 ctcaagcgat tgccgaagtc gctccaagtt tacttcggct tcctgtaggg ccttcgtctt
      961 tcgccgaaga tcgatacgtc cacgtatcca gggaggaaac aacaagatgg ccgctaccaa
     1021 aaggccgatc ataaaaccgg ccatgatggg tttgtaaatg ggagttgagg cttcgagcaa
     1081 tccaaagaaa tagcggagtg tgacttcctg ctcctggttt tgaagaaaga aggccaacga
     1141 aaggagcaaa agagtgccga ccagaatcag ccgtgtcatc gcggcgaagt ccttctacct
     1201 cctcggaggc gtggtcggac tgatcgcggg attggatata caattttctt gatttgagca
     1261 agaagtaaac gagagcgtgg acctcgcgct tcgacatggg cttttctggt cgttctggtc
     1321 cgatgggcga cctgcacttc cagccggtcg tccgggagcc attggggaaa ccggtctgcc
     1381 cactcaatgg caacgaccac ctcatccgtg aggtaatccg atagtccgat caactcgatt
     1441 tcttcaagtc tctgcaaccg atacaaatca atatgaacaa ccggaagccg cccctgatat
     1501 tcatggacaa acaggaacgt cggactcgtg acggatgcag ccggtgcgcc gagcccggca
     1561 acgatcccgc ggaccagtgc tgtcttaccg gcacccaatt caccgatcaa ggcgagcacc
     1621 tctcccccac gaagcagccg gccaatggca tgtccgaacg attctgtctc accgggagat
     1681 gagaggataa agtcgaggga gctgatagat gtagccatgg gagcgtcttc acaggagagc
     1741 attccatatt tcaatgggtt tgtcttttga tgaagcctga tggatgagcc tgagggcatg
     1801 gggaatctcc tcgatcacgt ctcccgcgat caacccggct tgccccgttc tgactgcagc
     1861 caaatcgccg gcagttccat gcagatacgt cgcggcacag gcagcctccc atgagggtat
     1921 gccttgggct aagagtccca ccatcatgcc ggttaacaca tcacctgttc ctgccgtcgc
     1981 cattccagga ttgccggtag ggcaaacagc gacggctcca tccggtcggg cgacgacggt
     2041 ccgagcccct ttcaagacga cgaacaggcc tcgctctcgc gcgaagcggg aggcaaaacc
     2101 gatccgatcg ctgttgacgg tctgaggcgt tgcgtcagcc tccagtcgtg ccatctctcc
     2161 cggatggggt gtgatgatcg gcggtgtttt gcaagacgcc aggagggcgg tccgccctgt
     2221 cagcgcgttc agggcgtctg catccaggac agccggccgg tcaagcagtt tcgtcaaggc
     2281 ctggacgagt tcaacggttt cgggatgagt ggataatccc ggaccgatgg cgatagcggt
     2341 tctggccgac atgaaggcga cgagccgatc caatgcggtc cgtgcaaagg tgcgcgcttt
     2401 ggtttcaggc attggaaccg tcatcacttc caacagcttt gattccaaga cgtcgttgat
     2461 acttgtcggg actgcaacag tcacgaggcc cgcgccgata cgaagggccg ctcgtgcagc
     2521 catggcggcg gccccggttt ttccaacgga cccggcaata atgccggcat gaccgaatgt
     2581 acctttgtga ctcgatggtt gccgtttcgg caggtatgct cgtaccacgt gttgtgtcat
     2641 caaggtcgtt cggctctcga ccgcgtcaag gtaggtcggc ggaattccga tgtccacgac
     2701 ggccacctct ccggccaggt cgatcccata gttctgatag aggcccaact ttggaagccc
     2761 aaacgtcacg gtgagagagg cacgaacggc tcgacccagg atagcgccgg tatcagcatg
     2821 gaggcccgat ggaagatcaa ccgccaccac gggacgactg gtttcgttta tgccgtcaat
     2881 ggcttcggca tagcgtccgg ttacagtcga ggagagtccc gtcccgagga gggcatcgat
     2941 gatgatatca ctgtcatgca aaagtgcttg ggcctgaatt cgggacgtat agaggtaaac
     3001 ggaagatttc cctgcgccac gaacaaattg cctatacatc gctgccgcat cgcggctcag
     3061 ttcggaggta ggtgccatgg cgagcacgcg caccttcgca tggcgccgac ggaataggcg
     3121 agcagcgacg aacccatcgc ccccattgtt gcctttgccg cacacaaccg tgaccgtctt
     3181 gccgcgcaca gatcccaagc gttgctcaag gcacgctacg acgccggacc cggcccgctc
     3241 catcagcgta gtcgccggga tgtgcgcttc cgaaatcgtt cggcggtcga gctcctgcat
     3301 ctgcgcggcg gtgatgatct tggtcatgat gaaccgaagg tggtgaacgg gcggactctg
     3361 tgagtcgacg acattcgatt agccgagcaa ggtcttcatc tctttcacgg cctgtacgag
     3421 accgacgagg accgcccgtg cgatgatgct gtgcccgatg ttgtactcca cgatctcggg
     3481 aatatgcgtg aggcgcttga tgttgcggta attcagtcca tgcccggcat tgaccccgat
     3541 cccgagcttg taggccagct tggcggcgtg ggtaatggct tcgaattccg catcggcttc
     3601 tttggaccgc gtggcattcg cgtaacgacc ggtatgcaac tccacgaaat caacggcaat
     3661 tttgtgtgcg gctctgatct gactcaaatc aggctcgaca aagacactga cggggattcc
     3721 cccattatgt aacagcgtca caatgttttg aattcggtca cgctgcccca caatatcaag
     3781 gccaccttct gtggtgagtt cctgccgctt ttccgggacc agcgtgacaa ggtc
//
LOCUS       DATNDD010000047         3657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4094090, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000047 DATNDD010000000
VERSION     DATNDD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4094090"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1126)
                     /locus_tag="VN647_01070"
     CDS             complement(131..1126)
                     /locus_tag="VN647_01070"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HWG95644.1"
                     /translation="MLDWFHFGRAKRRNVFGSFFILLGIILVPQSAGSTDSNLSIATQ
                     VSHTKSTLMSIQFRTPQNGWAVGTGGIILKTSDGGKKWKRVTSGTSALLTSVFFADSS
                     KGWATGAGGTLKYTTDGGESWVSQSLETQQPLYGVYFTSSKLGWVVGGGGTILHTADG
                     GEHWTEQVSGTTAALYAAHLLNDRKGTIVGALGTVLSTDDGGVTWTPQEAQNAVTLFD
                     VFFTDSTTGWAVGNAGAMFQTTDGGTKWVDQTLPCGSTCTRLTDLLKVRFTNPQEGWI
                     VGERGMLYRTIDAGITWSEGKSIVPTSLFGLSFSDTTHGWASGENGAILHLQLSR"
     gene            complement(1126..1947)
                     /locus_tag="VN647_01075"
     CDS             complement(1126..1947)
                     /locus_tag="VN647_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWG95645.1"
                     /translation="MSPAGAPIGTLLRINGEHFGFYSESGSTPYNFTDFDTGGNRVEI
                     GGVPAVIYRWNDDRIDVWVPFSAKTGKVVIYRSANTPNIGGLCCAERGTQATEAGDFT
                     LVTPVIESYEPQTAGLDATVTIKGRGFGTFLKTAEHADLGLNQKSYKRRGGLEINEPD
                     EHATVVSNVSRTEVLFNGTAALVQSWTDEEIVVTVPHRNLYGIGKKGEFFDNLATGPL
                     VVRRGSWDLLPDGTCCSPKKWVTLEAGPFTIEAKGLPVPDPDFWNYNTPDASTAQ"
     gene            complement(1944..2855)
                     /locus_tag="VN647_01080"
     CDS             complement(1944..2855)
                     /locus_tag="VN647_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWG95646.1"
                     /translation="MKQISILTAFLIWLVCFGDGANILIAQAGQRLIELSSRSSTPGA
                     TLVLNGKGFGSFKSTQFNKVTVNGVSALVQRWEADVIEIKVPFKATSGFVEVLMGKKK
                     LLAGFLTIVTPRIEAITPTEGERGTALQITGHHFGLSAGARDPNTMFGVNDVVVGGAV
                     VRPKRWKDDKIELEIPPNAVSGDVVVRLASSDPLPDGSCCAPVEYVVSNAVPLKLIPS
                     VRADPISGPVGTKVVLFGQGFGNAKELMDDAVLIGGRPVTIAQWKDDVIVFHVPLGAE
                     SGPLVLKHQGGNGCSRSLRFMFPVSSL"
     gene            complement(3049..>3657)
                     /locus_tag="VN647_01085"
     CDS             complement(3049..>3657)
                     /locus_tag="VN647_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG95647.1"
                     /translation="GLYSIQERMRALGGSFTIDSAPGHGTIATLIVPLVSSADDNQPH
                     PSVTESEDAWATVPADEADSLTKGRMMVSVLLVDDHAMVRQGLRSVLDAYADIQIIGE
                     ARDGVEAVKSVEKLRPQVVVMDINMPKMNGIEATMEIKTKWPETTIIGISVNVGDDSN
                     DAMQRAGAVTLLTKEAAVEQLHDTIVQAVGSQDTQGGSVIPR"
BASE COUNT          805 a         1121 c          937 g          794 t
ORIGIN      
        1 gtaatgagtg agaggaccac atcggctcaa gcctattctc tcagtattca cactctcaga
       61 aggtcctccc gcttcaagga ttgaaaactc aagttcctaa aagcggaggg cttttgcaac
      121 gaatgtggga ttagcggctc agttgcaaat gcaaaatggc cccattttcc ccgctggccc
      181 acccatgggt ggtatcggag aaactgaggc cgaataacga cgtagggacg atcgacttcc
      241 cctcgctcca ggtgatgccc gcatcgatcg tccgatagag catcccacgc tcgccgacaa
      301 tccaaccttc ttgcgggttg gtgaaacgca ccttcaacaa atccgtgagc ctcgtacagg
      361 tgcttccgca gggtaaggtt tgatcaaccc atttggtgcc gccgtctgtc gtctgaaaca
      421 tggcccccgc attaccgacc gcccagccgg tcgtcgaatc ggtaaaaaag acgtcgaata
      481 aggtcaccgc gttctgcgct tcttgcggcg tccaggtcac gcccccatcg tccgtcgaaa
      541 gcacggtgcc cagcgcgccg acgatcgtgc ccttccggtc attgagaaga tgcgccgcat
      601 agagcgccgc ggttgtgccg ctcacttgct ccgtccaatg ctcgccgcca tcggctgtgt
      661 gcaagatcgt ccctcctccc ccgacgaccc accccaattt cgacgatgta aaatagactc
      721 cgtacagcgg ttgctgagtc tccagggatt gagaaaccca tgactctccc ccatcagtcg
      781 tgtatttcag tgtccctcca gcccctgtcg cccagccttt tgatgagtcg gcaaaaaata
      841 cagacgttaa cagggcagaa gttccgcttg tcacccgctt ccacttcttc ccgccatcgc
      901 tggtcttcag gattattcct ccggttccga ccgcccaccc attctgaggc gtacgaaact
      961 gaatgctcat caaggttgat ttcgtgtgac tcacttgcgt agcgatggaa aggttggagt
     1021 ctgtgctgcc agccgattgg ggcaccaaaa tgatgccgag gaggatgaag aatgacccga
     1081 aaacgttccg tcgcttggcg cgaccgaaat ggaaccaatc cagcattatt gagcggtact
     1141 cgcgtcaggt gtgttgtaat tccagaaatc cgggtcggga acgggaagcc cttttgcttc
     1201 gatagtgaac ggtccggctt caagcgtgac ccatttcttc ggcgaacaac aggttccatc
     1261 gggaagcagg tcccaggatc cccgacgaac aaccagcggc ccggtggcca agttatcgaa
     1321 aaattcgccc ttctttccga tcccatagag gttgcggtgc ggtactgtga ccacaatttc
     1381 ttcatcggtc catgactgaa ccagggcggc tgtgccgttg aacaagactt ccgttcgtga
     1441 cacattcgac acaacagttg catgttcgtc cggttcgttg atttcaagtc cgccccgccg
     1501 cttgtaggat ttctggttca atccaaggtc ggcatgttca gcggtcttga gaaatgttcc
     1561 gaaccctcgg cctttgatcg tcaccgtggc atccaacccg gcggtctgag gttcatacga
     1621 ctcgatgacc ggcgtgacga gcgtgaagtc cccggcctca gtggcttgcg tccctcgctc
     1681 ggcacaacag aggccgccta tgttcggcgt gttggcgctc cggtaaataa ccaccttgcc
     1741 ggttttcgcg ctgaacggca cccagacatc gattcggtcg tcgttccagc gatagatcac
     1801 cgccggcact ccgccgattt cgacgcgatt cccaccggta tcaaaatccg tgaaattgta
     1861 aggagtcgac ccgctctctg aataaaaccc aaagtgctcg ccgttgatac gcaacaatgt
     1921 cccgatgggc gcgcctgcgg gactcataga gatgacacgg ggaacatgaa ccgtaaactg
     1981 cgcgagcacc cgttcccgcc ctgatgtttc agcacaaggg gaccggactc cgcgccgagc
     2041 gggacatgga agacgatgac gtcgtctttc cattgggcga tggtgaccgg ccgcccaccg
     2101 atcagtacgg catcatccat cagttctttg gcattcccaa acccctgccc gaacaagacc
     2161 actttcgtac cgacaggtcc actgatcgga tccgctcgaa cggacggaat cagtttcaac
     2221 ggaacggcgt tgctgacgac gtactcgacc ggagcacagc atgatccgtc cggcagcggg
     2281 tcggaggaag ccagccgaac tacgacatca ccgctcaccg cgttcggcgg aatttcaagc
     2341 tcgatcttgt cgtctttcca tcgcttgggg cgcaccacgg ccccaccgac caccacgtca
     2401 ttgacaccga acatcgtgtt cggatcgcgc gcacctgccg agaggccgaa atgatggccg
     2461 gtgatctgaa gagcggttcc ccgttcacct tccgtcggtg tgatggcctc gatccgcggc
     2521 gtaacaatgg tcaaaaatcc ggcgagcagc ttcttctttc ccatcaacac ctcgacaaag
     2581 ccgcttgtcg ccttgaaggg aaccttgatc tcgatgacgt ctgcttccca tcgctgcact
     2641 aaagctgaga ccccattgac ggtcactttg ttgaattggg tcgacttaaa gcttccaaac
     2701 cctttcccgt tcaggaccaa ggttgcgccg ggagtagaag accgagagga caattcaatg
     2761 agcctctgac cggcctgtgc aatcaagata ttcgctccat ccccaaaaca caccagccaa
     2821 atgagaaatg cggtcaagat agagatttgt ttcatggaca gcctaccgga aaagggaaaa
     2881 gacatacaga ggttcacagc acaatattcc cgactttaac aagccatttt ttcgggagtc
     2941 aaggaggtaa cggacaatcg gaccgggcac tgacctctat tcgttcagcc gacactctgg
     3001 tcgacgcggg acaccaagat agcatcccta aaaagagcct gagaagatct atcgcggaat
     3061 caccgaacct ccttgcgtgt cttgtgaacc gacagcctga acgatcgtat cgtggagttg
     3121 ctccaccgct gcttctttgg tcagcagtgt gaccgctccg gctcgctgca tcgcatcgtt
     3181 gctgtcgtcc ccgacattca cggaaatgcc gatgatcgtg gtctccggcc acttggtttt
     3241 gatctccatc gtagcttcaa tcccgttcat cttcggcata ttgatgtcca tcaccaccac
     3301 ttgaggtcgg agcttctcga ccgatttcac cgcctccacg ccgtctcggg cctctccgat
     3361 gatctgaata tccgcatagg catccagcac cgatcgcagc ccttgccgca ccatcgcatg
     3421 atcatccacg agcaacaccg acaccatcat ccggcctttc gtgaggctat cggcctcatc
     3481 ggcaggaact gttgcccagg catcctcgga ctcagtgact gaaggatgcg gctggttatc
     3541 gtccgcgctg ctgaccagcg gcacgatcag tgtcgcaatg gttccgtgcc caggagcgga
     3601 gtcaatggtg aacgagccgc ccaatgcccg catccgctcc tggatgctat ataaacc
//
LOCUS       DATNDD010000048         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3021660, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000048 DATNDD010000000
VERSION     DATNDD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3021660"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VN647_01090"
     CDS             complement(<1..378)
                     /locus_tag="VN647_01090"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWG95648.1"
                     /translation="MKSVLTVAVTITAGIVLSGCVLTQKMMPGTMSSSEIFGVLDTIN
                     RSEIDAGKLAKEKATAPEVRSFASHMVSEHERMMEDMKQLARRMDVQPQKPALAFTLN
                     ASHQDAMEELRDKSGSDFDKAYIE"
     gene            804..1211
                     /locus_tag="VN647_01095"
     CDS             804..1211
                     /locus_tag="VN647_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95649.1"
                     /translation="MMTSKVARLLSGGILLLGLSTMPQVGDVASAADKLKETPCEVSE
                     GTNAMQAEQAATRDSHVVFGEVVRVDGSTYVVKEKGGKEVSVKTDEKTEQSPIKKGDH
                     ISANVDKNNHALWIRGNRGTDRRTEHVSGDCNP"
     gene            1432..1566
                     /locus_tag="VN647_01100"
     CDS             1432..1566
                     /locus_tag="VN647_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95650.1"
                     /translation="MIIAAGQGATEGPVINRDLFKEDLRRHALRCAERAEPMDVRIAL
                     "
     gene            1618..>2908
                     /locus_tag="VN647_01105"
     CDS             1618..>2908
                     /locus_tag="VN647_01105"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWG95651.1"
                     /translation="MISQWLKWTVVSLAVLMGVLSTAAFFADEPLRRYGERTVNEAVE
                     GYSFKIGTLAVHPLALSVDLGDVVVRQEAHPDPPLISIPEVVVDARFAPLLNGKAKGD
                     IRLKNPIISVTQQQINTASAEADQRTEQMVSWQDRVRGMIPFEATLFIKNGEVRYVGQ
                     RNAEPIRVTALDIEARYLTNRPQDAQAYPAELVMRSRLFDAGYLEFNGLADLLNKPLP
                     MVDASVKVSGLRIADLMPIVGAYHVVVNRGTFSATGRIMHEKQMVLSLESFVLGGAKV
                     AYVYQEETESKQRRQLERGAERAAKAHRDPWFVLKVGHGKIFNSEVEFVNRSTDPAYR
                     VFLTDVNADIDNFSNRLEEGTGVVKITGKFMGSGPTVVTGTFRPEKPRPDFDLAVKII
                     KTDVTTLNHVLRAYGDTDTHHGKFALFSELTVKNNRID"
BASE COUNT          695 a          735 c          847 g          631 t
ORIGIN      
        1 ctcaatgtac gccttatcga agtccgagcc ggatttgtcc cggagttcct ccatcgcgtc
       61 ctgatgagaa gcgttcaacg taaaagccag cgccggcttt tgcggctgta cgtccatgcg
      121 tcgggccaac tgcttcatgt cctccatcat cctctcgtgt tcactgacca tgtgagaggc
      181 aaacgagcga acttccgggg cggtggcctt ctccttagca agcttaccgg catcgatctc
      241 gctgcgatta atcgtatcga gcacgccaaa aatttcggaa ctcgacatgg tgccgggcat
      301 cattttttgg gttaacacgc agcctgagag cacgatcccg gcggtgatgg taacggcgac
      361 ggtcaatact gatttcatga caacctctct tcttatgagt cgtcggtggt taaggaatgc
      421 ataccaatca tgtctatgca agacaagagc ctactaggct tacgcggttg atcgttctgc
      481 tctgttgttg tgatggcatt cggagagact caggttgagg tttgggttga gtggacccgg
      541 aggcatcctc acctcatcct taaccccagg ctcaaacagg tagtcaggga ggccaagtgt
      601 cctcaagtcg gacgaactgt ctggccatag ccaacgatgg gtgacgcgga aaactgatac
      661 gaggggaatc accagttggt tcgcgacaga cttgagccga tgatcgatca tagggcgtgt
      721 cgatcgtcgg tgattcatgt gcgaaacccg cgcaaaggaa tgcgcatact gcgtgagacc
      781 gccctcactt aaccaggagg catatgatga cttcaaaagt tgctcgattg ttgtcaggcg
      841 gaattttatt gctcggacta tccacgatgc cgcaggtcgg cgatgtcgca tccgcagcag
      901 ataagctaaa ggaaacccct tgtgaagtat cagaaggtac aaatgctatg caggcggaac
      961 aagccgcgac gcgtgacagt catgtcgtgt tcggtgaagt cgtacgcgtg gacggctcga
     1021 cctacgtggt taaggaaaaa ggtggcaaag aagtgagcgt gaagaccgac gagaagacgg
     1081 aacaatctcc gatcaagaaa ggagatcaca tttcggcgaa tgtagataag aacaatcatg
     1141 ccctgtggat tcgagggaac agagggacgg atcgtcgaac cgagcatgtc tcgggtgact
     1201 gcaatccgtg accatgtgca gaactattgg ggttccctga cggcatcgct ggcgcggggt
     1261 tgtgggcaaa tcaaccgctt caatgtcgaa gttatggaag atgatgttca tgcactgaga
     1321 cggatcagca agagttggtt cgaagtgtcg agtgtccgaa aggctatatg gccaaacggg
     1381 tattattggc gacggggctg acgcacctcc cacccaacat tccaggagtg catgatcatt
     1441 gccgccggtc agggggcgac agaggggccg gtcatcaacc gggatctctt caaagaagat
     1501 ttgcggcgcc atgcccttcg gtgtgcagag agggcggagc cgatggacgt gcgcattgca
     1561 ctatgaccag tacggctgaa tctgcatacc cggatataga gctgctgttg cgcaatcatg
     1621 atatctcagt ggcttaaatg gacagtcgtc agtctcgccg ttcttatggg agttctctca
     1681 accgccgcct tctttgcaga tgaacctctt cgccggtatg gggagcgaac cgtcaacgaa
     1741 gcggtggagg gatatagctt caagatcggc acactcgccg tacatccgct cgccctatcg
     1801 gtggacctgg gagatgtggt ggtgcgacaa gaggctcatc cggatcctcc gctgatcagt
     1861 attccagaag tcgtcgtcga tgcgcgattc gcgcctctgt tgaacggaaa ggcgaagggc
     1921 gacattcgtc tcaaaaaccc gattatttcc gtcacacagc aacaaattaa cactgcctcg
     1981 gcagaggcgg accaacgaac tgagcagatg gtctcttggc aagatcgggt tcgaggcatg
     2041 atccctttcg aagcgacact tttcatcaag aacggggagg tgaggtatgt cgggcagcga
     2101 aatgccgagc caatccgagt tacggcactg gatattgagg cgcgctatct cacgaatcgt
     2161 ccccaagacg cgcaagccta tcccgctgaa ctagttatgc gcagtaggct gttcgatgcc
     2221 gggtatctgg agttcaacgg tctggcggat ctactgaaca agccgcttcc catggtcgat
     2281 gcgtccgtta aggtgagcgg gctgcgcatc gccgatttga tgcccattgt cggagcctat
     2341 cacgtggtcg tgaatagagg cactttcagt gcgacaggac gaatcatgca tgagaagcag
     2401 atggttttgt cactcgaatc gtttgtgctg ggaggggcaa aggtcgccta tgtctatcag
     2461 gaagagaccg aatccaagca gcgtcgtcaa cttgagcgtg gagcggaacg agcggcaaag
     2521 gctcaccgcg atccgtggtt tgtgctcaaa gtcgggcatg ggaaaatatt caacagcgag
     2581 gtggaattcg tcaaccgctc gaccgatccg gcctatcgtg tcttcttgac ggacgtgaat
     2641 gccgacatcg acaacttcag caatcgactc gaggaaggca ccggcgtcgt gaagatcacc
     2701 ggcaagttca tgggcagcgg tcctacggtc gtcaccggca catttcggcc ggaaaagccg
     2761 agaccggatt tcgatctggc cgtgaaaatc atcaagaccg acgtgaccac gctcaaccat
     2821 gtcttgcggg cctacggtga taccgatacc caccacggca agtttgcgct gttcagcgaa
     2881 ctgacggtga aaaacaatcg tattgacg
//
LOCUS       DATNDD010000049         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3859210, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000049 DATNDD010000000
VERSION     DATNDD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859210"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VN647_01110"
     CDS             complement(<1..238)
                     /locus_tag="VN647_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWG95652.1"
                     /translation="MKTRTNWMQAIVIASLLGCFPRGVEAETARWDIDPDHSIIEFRV
                     AHMVVSKTSGRFLNYRGFVDLDADTKAWKTIEATI"
     gene            482..1516
                     /gene="tdh"
                     /locus_tag="VN647_01115"
     CDS             482..1516
                     /gene="tdh"
                     /locus_tag="VN647_01115"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HWG95653.1"
                     /translation="MQALVKTTAGPGLTLIDWVDPIPGPHDAVVKVAATSLCGTDVHI
                     YRWDEWAQRRIHPPRIIGHELCGHVVEIGREVSLVKVGDYVAAESHLTCGACFQCRTG
                     QAHVCKNYKILGIDRDGSYAQYVALPEGVLWHTAPGIPPALACVQEPLGNAVDAALAE
                     DLTGHTVLITGCGPTGLFAAAVARTAGAATIIASDVSDYRLGLAKQVGADHVLNVRAE
                     SPDRVAAAILEMTAGEGVDAALEMSGDPTAMHQAFRAVKNGGRVTLFGIPTGPVCFDL
                     PNEVIFKGIRVYGITGRRLFGTWYRLAGLFKAGLDIRPVVTHSFPLGEFATGFELIRS
                     GQCGKVVLIP"
     gene            1568..>2116
                     /locus_tag="VN647_01120"
     CDS             1568..>2116
                     /locus_tag="VN647_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWG95654.1"
                     /translation="MAYSSFKKALENQLADIKSQGLYKAERRLLSPQGSDIRVAQGSV
                     LNLCANNYLGLANHPAVVQAATKGLETHGYGMASVRFICGTQDLHKQLEQAISEFLGT
                     EDSILYSSCFDANGGLFEVLLDEGDAVISDALNHASLIDGIRLCKAKRFRYAHSDMAD
                     LEAQLQAAGGCRLRLIATDGVFS"
BASE COUNT          432 a          588 c          608 g          488 t
ORIGIN      
        1 tgatcgtggc ttcaatcgtc ttccaggctt tcgtgtccgc gtccagatcg acaaatcccc
       61 gatagttcag gaaacgcccc gacgtttttg acaccaccat gtgggccacg cgaaactcga
      121 tgatcgaatg atcaggatcg atgtcccaac gagccgtttc cgcctcgacg ccccgcggga
      181 agcatcccaa tagactcgct atgacgattg cctgcatcca gtttgtccgg gttttcatat
      241 catcaccttt cccctgtcgg cttgtgtgaa cggacaccta cctcttgggc gatgccacgg
      301 cttgaacggc atcctactca tcgctgggaa tcccatgcaa cccgtttctt tcacccgcct
      361 atgtaggaag ccgcacttcg gcgaggtgtc accgaagaac catgggagac catgatttta
      421 tagctgtggg gactccaccg aaaatgggtg tcaaataacc gagttggtat agaataggtt
      481 catgcaagca ctcgtgaaaa ccacggcagg gccgggatta accctcatcg attgggtgga
      541 cccaatcccg gggccacatg acgctgtggt gaaggtggct gcgacctcgt tatgtggaac
      601 cgacgtccat atctatcgtt gggacgaatg ggcgcaacgg cgaattcatc caccacgaat
      661 tatcggccat gaattgtgcg gccacgtcgt ggagattggg cgggaggtat ccctggtgaa
      721 agtcggcgac tatgtcgccg ccgaatcaca cttgacctgt ggagcgtgct ttcagtgtcg
      781 caccggacag gcgcatgtgt gcaaaaacta caagattctt gggatcgatc gagacggctc
      841 ttacgctcag tatgtcgcac tgcctgaagg tgtcttgtgg cacacggctc cgggaattcc
      901 tccggcgttg gcctgtgtgc aggagccgct tggtaatgcc gttgatgctg ctctcgctga
      961 agatctcacc ggtcataccg tgttgatcac ggggtgcggc ccgaccggtt tatttgccgc
     1021 ggctgtcgca cgtaccgccg gcgctgcgac catcatcgca tccgatgtca gtgactatcg
     1081 gcttggcttg gcgaagcagg ttggcgcaga tcacgtcctc aacgtgagag ccgagtcacc
     1141 ggatcgagtg gcggcagcca tcctcgagat gaccgctggt gaaggggtcg atgcagcact
     1201 ggagatgtct ggcgatccca cggctatgca tcaagccttt cgcgcggtga agaacggcgg
     1261 acgagtaacc ctgttcggca tcccgaccgg tcccgtttgc tttgatctgc cgaacgaagt
     1321 tatttttaag ggcatccgtg tctatgggat caccgggcgg cgcttgttcg gaacctggta
     1381 tcggctggcc ggtctcttca aggcaggtct cgatattcgg ccggtcgtga cccattcctt
     1441 tccgctgggc gaatttgcta ccgggtttga gttgatccga tcagggcagt gcggcaaggt
     1501 tgtcttgatc ccgtaatagg actgagagat cggccctcag tcctcatctc tcagtcctgt
     1561 tctaaccatg gcctattcgt ccttcaagaa agcccttgaa aatcagcttg ccgatatcaa
     1621 atcccaaggt ctgtacaagg ccgaacgccg attgctgagc ccgcaaggtt ccgacatccg
     1681 agtcgcgcaa ggctcggttc tgaacctctg tgcaaacaat tatctagggc ttgcgaatca
     1741 tccggccgtc gtgcaggccg ccacaaaggg attggaaacg catggatatg gcatggcttc
     1801 cgtgaggttt atttgcggca cgcaggattt gcacaagcaa ctggagcagg ccatcagtga
     1861 gtttctcggc accgaggact caatcttgta cagctcctgc ttcgatgcca atgggggact
     1921 attcgaagtg ctgttggatg agggtgatgc cgtcatcagc gatgccttga accatgccag
     1981 cttgatcgac ggcatcaggc tctgtaaggc caagcgattc cgatacgccc attccgacat
     2041 ggcagacctc gaagcgcagc tccaggcagc cggtgggtgc cggctacgtc tgattgcaac
     2101 cgatggggtg ttctcg
//
LOCUS       DATNDD010000050         4451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2992722, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000050 DATNDD010000000
VERSION     DATNDD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992722"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VN647_01125"
     CDS             complement(<1..1175)
                     /locus_tag="VN647_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HWG95655.1"
                     /translation="MATVVVIGGGPTGLMAAEAAVTAGATVELYDAMPSVGRKFLLAG
                     KGGLNLSHSEPMESFLSRYGTRRVFIEPAIRSFPPAALRAWARELGVETFVGTSGRIF
                     PTDLKAAPLLRAWLRRLRQADIQFHIRHRWCGWDQEGSLLFTTPYGPVHAKADAVVLA
                     LGGGSWPHFGSDGAWVSILSERKIPIAPLKPANCGFDVQWSEHFRTKFSGYPVKTVEV
                     AVKTVDGIVTRHRGEFVITANGVEGGVIYMVAAAARNVIAAEGTVTLWVDLVPDRTLQ
                     RLTKDFAEPRGKRTVATHLKRCAGIAGVKSGLLRDVLSKDVPADPVRLAAVIKSLPLT
                     LVAPRPLEEAISTAGGVSFDALDRRLMLRSFPGVFCAGEMLDWEAPTGGYLLTGCLAT
                     "
     gene            1452..2453
                     /locus_tag="VN647_01130"
     CDS             1452..2453
                     /locus_tag="VN647_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWG95656.1"
                     /translation="MRVRATCVAMFGLASLLSGAVEQVLAAEDLSQRNNLHVNKPNHP
                     RFPDDVFSGMFPGLPPYAPPTDEAREQAKKLGAKGGVIDASDLLTDPKESIANPGPNN
                     PDNPNMTAGFTFFGQFLDHDLTLALKAPLLEQTHPRRTTNFRTAEFDLDSVYGDGPER
                     SPELYETSSGNIKFRIEAIPGSEQVSRKGAVRYDLPRDPNNNAIIADGRNDENLVISQ
                     FHLAMLKFHNAVVDRLRSNPAQAHRSARDIFKEARRQVQWHYQWIIVNEFLPLTIGQD
                     RVDEILRNGPCFYRVHDRTHDSLFLSSRREPLIPVEFAVGAYRFGHSQVRPSYRLNF"
     gene            2478..3074
                     /locus_tag="VN647_01135"
     CDS             2478..3074
                     /locus_tag="VN647_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95657.1"
                     /translation="MFIFDDSADPNDSDPNDMRGGKRAPRRYIDWQTFFKFDENFKPN
                     KRIDTELSSPLMHLLGSRGPAPGMPSDGIQSLASRNLMRHVNYGVPSGQAIARVMGAQ
                     VLTPEQLAELAPFGMERSTPLWYYILKEAEVFENGVRLGPVGGRIVGEVFIGLLKADK
                     DSYLVVDKNWKPTLPSAKSGDFEITDLLRFAGVVHPLN"
     gene            complement(3133..3828)
                     /gene="sigZ"
                     /locus_tag="VN647_01140"
     CDS             complement(3133..3828)
                     /gene="sigZ"
                     /locus_tag="VN647_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HWG95658.1"
                     /translation="MSRLLAEHKPEQYRMDADGGEVIIMKMTEELWQVMHDGLRAFIA
                     GRVNDQGHVDDILQDVFVRVYRQLDTVRDPHRIIAWIYQVTRNAIIDHYRKPGRQREV
                     PSGLSSELEVLHEAPAISEMTRQGDATELRAELAGCLRPMIERLSQDYRDAITLVEIE
                     GLTQQIAAKQMGISLSGMKSRIQRGRGQLKQMLNDCCVIELDGRKGIVEYHSRRAECD
                     PCVPNKNMPETDL"
     gene            complement(3803..4246)
                     /locus_tag="VN647_01145"
     CDS             complement(3803..4246)
                     /locus_tag="VN647_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HWG95659.1"
                     /translation="MRLHISLDVRDVSKSVAFYEKVFGISPQKQMTDYAKFDLITPAL
                     NFSLVSSTGEVSAVDHLGIEVETVEEIAEWKARLQREGILKRVEDNIACCFARQDKLW
                     LSDPDGNAWEIFTVHEQLEVTGRMSQAGCCVPKKAGAHEPATCGT"
BASE COUNT         1008 a         1311 c         1127 g         1005 t
ORIGIN      
        1 gtcgccaaac aacccgtgag cagatatcct ccggtcggtg cctcccagtc caacatttct
       61 ccggcgcaaa acaccccggg aaatgaacga agcatcaatc tcctgtccag tgcgtcgaac
      121 gagacgccac cggccgtact gatggcttct tccaaggggc gaggggctac gagcgtgagg
      181 ggtagagatt tgatgacagc ggcgagacga acaggatctg ccggcacatc tttcgacaga
      241 acgtcgcgca ggagacccga cttgactccg gcaatacccg cacaccgttt gagatgcgtc
      301 gcaaccgtac gtttaccacg cggctctgca aaatcctttg tgagccgctg aagcgttcga
      361 tccggaacga gatctaccca taacgtgacc gttccctccg ctgcgatcac attacgggcg
      421 gcggctgcaa ccatatagat gacgccgcct tccacgccgt tcgccgtaat gacgaactcc
      481 cccctgtgac gggtcacgat gccgtccacg gtcttcacgg cgacttcaac tgtcttcact
      541 ggatatccgg aaaacttcgt ccgaaagtgc tcgctccact gtacatcaaa tccacagttg
      601 gccggcttca gcggagcgat aggtatcttt cgctcactca ggatcgaaac ccatgctcca
      661 tcggaaccga aatgaggcca gctacctccg cctaacgcca gcaccacagc gtcggcttta
      721 gcatgcaccg gtccatatgg agtggtaaaa agcagcgatc cttcttgatc ccacccgcac
      781 caccgatgtc tgatatgaaa ctgtatgtcg gcttgtctca accgacgcaa ccatgcacgc
      841 aggagtggtg ctgcttttag atcagtggga aagattcgtc ctgacgtacc gacgaacgtt
      901 tccacaccaa gctcacgagc ccaagcccgc agagctgcag gaggaaatga tctgatcgct
      961 ggttcgataa acacccggcg agtgccatag cgcgaaagga aggactccat cggttccgaa
     1021 tgagagagat tcaagccgcc tttccctgcc aacagaaact ttcggcccac cgacggcatg
     1081 gcatcgtaca gctcgacggt agcgccggca gtcaccgctg cttccgccgc catgagtccg
     1141 gtcggtccgc cgccgattac caccaccgtt gccatcagaa cgttactgcc ctactacaag
     1201 aagctcgcgt ctggcgaatg gtccataatt gtatggccac aactacaact ccaactacat
     1261 ttgtacagtc tactctgaaa cgagcgctgc atttgtacat atctcgcgta attctcgcac
     1321 atcactgttg gtctgtttct tgccggcaca cgacactcga aaaacgatgg ccatcgcagt
     1381 aagtcgtata cctatttaat tcacgagacc gacctgttga cgtgacgcag gtcttcagag
     1441 gaggaatgct catgcgcgtt cgagcgacat gtgttgctat gtttggtctt gccagcctgt
     1501 tatccggcgc tgtcgagcag gtactcgcgg ctgaagatct gtcgcaaagg aacaacctgc
     1561 atgtgaacaa acctaaccac ccacgatttc cagacgatgt gttcagcggc atgtttccag
     1621 gccttccgcc ctatgcccca ccgaccgatg aagcacggga gcaggcgaaa aagctcggcg
     1681 ccaaaggtgg agtcatcgat gcctcggatc tcctcacgga tccgaaagag tcgattgcaa
     1741 atcctggccc aaacaatccc gacaatccga acatgacggc aggcttcacg ttcttcggtc
     1801 agttcctcga tcacgatctg acgctcgcgt tgaaagcgcc gctgctcgag caaactcatc
     1861 cccggcggac gacgaatttt cgcaccgccg aatttgacct ggatagtgtc tatggtgacg
     1921 gtcccgagcg atcacccgaa ctgtatgaga caagttccgg caatatcaaa ttccgcatcg
     1981 aagccattcc cggttccgaa caagtgtccc gaaaaggcgc ggtacgatac gatctcccgc
     2041 gcgatccgaa caacaatgcg atcatcgcgg acggacggaa cgatgaaaac ctcgtcatca
     2101 gccagtttca cctggcgatg ctgaagtttc acaatgccgt cgtcgatcgg ttgcgctcca
     2161 atccggcaca ggcgcatcgt tcggccaggg acattttcaa agaagcgcgt cgccaggtgc
     2221 aatggcacta ccaatggatc atcgtcaacg aattcttacc gttgacgatc ggacaagatc
     2281 gggtggatga gatcttgcgg aacgggccgt gtttctacag agtccacgac cgaactcacg
     2341 attcgttgtt cctcagttcg agacgagagc ccttgatccc cgtggagttc gcagtgggtg
     2401 cgtatcgatt cggccattcg caagttcgtc ctagttatcg cttgaatttc tgagccgatg
     2461 ccgcgtctca agtctttatg tttattttcg acgattcagc agatccgaac gattccgatc
     2521 ccaacgacat gcgcggcggc aagcgggccc cgcgtcggta catcgactgg cagaccttct
     2581 tcaagttcga cgaaaacttc aaacccaaca agcggatcga caccgaactg tcgagccccc
     2641 tcatgcattt gctcggatca cgtggtccgg cacccggtat gccctcagat ggaatccagt
     2701 ctctggcctc gcgtaatctc atgagacatg tcaattacgg cgttccgtcc gggcaagcga
     2761 tcgctcgggt gatgggcgct caggtgctca cgccggaaca gctggccgaa ttagctccct
     2821 tcggcatgga acgaagcacg ccgctctggt actacattct gaaagaggcg gaggttttcg
     2881 agaatggtgt gcgattggga ccggttggag gccgtatcgt cggagaagtg ttcatcggct
     2941 tactcaaggc cgataaggac tcctatctgg ttgtcgataa gaactggaag ccgaccttgc
     3001 cttccgcaaa atccggcgat ttcgagatta cggatctctt gcggttcgcc ggcgtcgttc
     3061 atccgctcaa ctagatcctg ccttgtctcg ttgggtaccc tctactcaga gggtacccaa
     3121 ctcatccaat attcatagat ccgtttccgg catgttcttg ttaggtacac agggatcaca
     3181 ctcggctctg cgcgaatgat actcgacgat gccttttcgt ccgtccaact cgatcacaca
     3241 gcagtcattg agcatctgtt tgagctgccc gcgccctcgc tggatcctgg atttcatgcc
     3301 ggatagtgag atgcccatct gtttggccgc gatctgttga gtcaatccct cgatctctac
     3361 aagggtgatt gcgtctcgat agtcctgtga caatcgctcg atcatcgggc gaagacaacc
     3421 ggcgagttct gcgcgaagtt ccgtagcatc accttgacga gtcatctctg agatcgcagg
     3481 agcttcgtgc aatacttcaa gttcagaact caatccagat ggaacctctc gttgtcttcc
     3541 tggcttccgg taatgatcga tgatcgcatt ccgtgtgact tgatagatcc atgcgatgat
     3601 ccggtgagga tcgcgtaccg tgtccagctg tcggtagacc cgcacaaaca cgtcttgcag
     3661 aatatcgtcg acatgcccct gatcgttcac acgtccggcg atgaaggcgc gaagaccatc
     3721 atgcatgact tgccacagct cttccgtcat cttcattatg ataacctctc ccccgtcggc
     3781 atccatacgg tactgctccg gcttatgttc cgcaagtagc cggctcatgc gcacctgcct
     3841 tcttcggcac gcagcagccg gcttggctca ttcgtcctgt gacctctaat tgctcatgga
     3901 ccgtaaagat ctcccaggca ttgccgtccg gatcggaaag ccaaagttta tcctgtcggg
     3961 caaaacaaca ggcgatgtta tcctccactc ttttcagtat accttcccgt tgtaaacggg
     4021 ccttccactc cgcaatttcc tcgaccgtct cgacctcaat tcccaaatga tccacggcac
     4081 tcacctcccc ggtggatgaa accagcgaaa agtttagggc cggggttatc aggtcaaact
     4141 ttgcatagtc ggtcatctgt ttctgcggtg atattccaaa cactttttca tagaacgcca
     4201 ccgactttga tacatcacgg acatcaagag aaatatggag acgcatggga aacctccttg
     4261 ttctcgagta gcggatgatt ccgcactcag ctctatagac ggaggaggtt agaaaaggac
     4321 gcaatgaaga aaggcggtgt tcggcttttc accaaggcct ggtgccgtca gtccggtatg
     4381 ggacgcggtg cgccgctcga atccaagact tcgcggagct ttttcgtcag catgtccggc
     4441 gtatagggct t
//
LOCUS       DATNDD010000051         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2994233, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000051 DATNDD010000000
VERSION     DATNDD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2994233"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VN647_01150"
     CDS             complement(<1..805)
                     /locus_tag="VN647_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HWG95660.1"
                     /translation="MIAKRSVRHKTSSPVMSDRWDLTHLAKNPLNDLDRHLKALDAQV
                     VQVESARPQLQATMASSDFRSILRLTESIAESSARLGAFAYLWFSEDTKNTKARSFKS
                     QVEERLTAVHNRLLFLDLWWQGIDNENAARLMADADDLRYYLETIRRYTPHTLSESEE
                     KIINVKNVTGRSAVNTLYDVMTNGLTFTMKIGGKARTMNREELMSHVRNAKASIRQTA
                     YRELYRVFSAQRDLLGEIYRTLVNDWKAENVGLRGFASPIATRNLGNDVP"
     gene            951..1283
                     /locus_tag="VN647_01155"
     CDS             951..1283
                     /locus_tag="VN647_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95661.1"
                     /translation="MNDHELHRVVQYVTASTSYARDTVSDILHTGLGELSSLAAHSTR
                     PFEREALLEYVSQWTIKRTGQPEPLVREVLGCAGRWLDEVYNECVDRQPEVLGESPDS
                     DEGAEPVP"
     gene            1337..2179
                     /locus_tag="VN647_01160"
     CDS             1337..2179
                     /locus_tag="VN647_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95662.1"
                     /translation="MARWRPRRTMRVHSWYDSSGPAVVWKRHLPQESSRQLSDNWSLL
                     HRHCADHSSHLRLGRLLHRLTHHDLGVQESALAELELATQLIRVGLRVTFLPESQART
                     ADLECRLGSERFFVEVTAMVGSAERRLLPMRGIMRDEEPEEEADRSVILTHRVLARIS
                     QKAKQLVDYCEPVNLSISIPRADLRGGRQGRREQIRMDLKALVGSITVDLTRLRHLSA
                     VLISLWDVEPLPSKAGTRLANVELVERTRHQRTHPRVRMFIQNPAAAASLSERQADVV
                     QLIL"
BASE COUNT          539 a          660 c          724 g          582 t
ORIGIN      
        1 tcggcacatc attgccgagg ttgcgggttg cgatgggaga agcaaaaccc cggagtccga
       61 cgttctcagc cttccagtcg ttcacgagtg tccgatatat ctctccgagc aggtcacgtt
      121 gggctgagaa cacgcgatag agttctctgt acgcagtctg gcgaatggat gcctttgcgt
      181 tccgcacatg gctcatgagc tcttcgcggt tcatggtgcg tgccttcccg ccgatcttca
      241 tcgtgaatgt cagtccgttg gtcatgacgt catagagtgt gttgacggcg ctccggccgg
      301 tcacattttt gacattgatg atcttttcct ccgattccga aagtgtatga ggtgtataac
      361 gtctgatggt ctccaggtag taccgaaggt cgtcggcgtc ggccatcagc ctcgccgcat
      421 tttcgttatc tatcccctgc caccagaggt cgagaaagag caggcgattg tgtacggcgg
      481 taagccgctc ctccacctgc gacttaaacg agcgcgcctt ggtgtttttg gtgtcttccg
      541 aaaaccagag gtatgcgaat gcgccaagcc gggcagagct ctctgcgatc gactcggtga
      601 gcctcaggat cgatcggaaa tcagagcttg ccattgttgc ctggagctga ggacgagccg
      661 actcaacctg tacaacttgt gcatcgagcg ctttcaaatg ccgatccaag tcattcagtg
      721 ggttcttggc gagatgggtg aggtcccacc ggtcagacat gaccggggat gaagtcttgt
      781 gccgaaccga ccgtttcgca atcatgccca atctcctttg cgtagctgcc aagcgatgag
      841 gagattgcat cttatcatca gactgtgccg tgattgcaga aaatcatcgg aatcaaagga
      901 agtccggccg cattgacagt cagtcatctg ggtgtaaaga ttgtttcaat atgaacgacc
      961 acgaactcca tcgcgttgtt caatatgtga ccgcgagtac atcgtatgcc cgggacacag
     1021 tgtcggacat cctccacaca ggtcttggag agttgtcttc cctggccgcg cattcgaccc
     1081 gacctttcga gcgggaagcg ctcctcgaat atgtttcaca gtggacgatc aaacggacag
     1141 gccagccgga gccgttggtg cgtgaagttc tgggttgtgc gggtcggtgg ctcgatgaag
     1201 tgtacaacga atgtgtcgat cgacaaccgg aagtcttggg cgaatcaccg gatagcgacg
     1261 aaggcgccga gccggtcccc taaacgactt aggtcgctcg tgaagcgacg cttcatgagc
     1321 cggaatggag aaagtgatgg cacgctggcg gccacgacgc acaatgcgag tccattcctg
     1381 gtatgactct tccggtccag cggttgtttg gaagcgtcat ttgccacagg agtcttcacg
     1441 ccaactcagt gataattgga gtctcctgca tcggcactgt gccgatcact caagccatct
     1501 ccggctcgga cggcttcttc atcgcttgac ccatcatgat ctcggtgtgc aggaatctgc
     1561 gctggcagaa ctggagctgg ccacgcaact gattcgcgtc ggcctccggg tcacgtttct
     1621 gcctgagtcg caagccagaa cagccgattt ggagtgtcgt ctgggatcag aacggttctt
     1681 tgtcgaagtg accgcgatgg taggttcggc tgaacgacgg ctgctgccta tgcgcgggat
     1741 catgagagat gaggagcccg aagaagaagc ggatcgcagt gtgatcttga ctcaccgagt
     1801 cttggcgcgc atcagccaaa aggcaaagca gcttgtcgac tattgcgagc cggtcaactt
     1861 gagcatttca ataccgcgag ccgacctccg gggaggaaga cagggacgtc gagagcagat
     1921 tcgaatggat ttgaaggcgt tggttggttc cataacggtc gatctgacga gactccggca
     1981 cctcagcgcc gtgttgattt cgctctggga tgtcgagccg ttgccgtcga aggccggaac
     2041 taggttggca aatgtggaac tcgtggagcg aaccaggcat cagcgaacac acccgcgtgt
     2101 gcgcatgttc atccagaacc ctgccgccgc tgcgtcgctg agcgagcggc aagcggatgt
     2161 cgttcagctg attctctgat gaaggctatc cggaatcgct ttcatcaagg gccataaaag
     2221 actcccggta ccttttgata ccgtaaaaat tcccttcatc acctcttgcc tttattgaag
     2281 gcttgtataa tcaccagcgg ctccatcaga gtctccgcta cgtgagccgg ctggagttcg
     2341 agcaaggttc ggtggggtca cggtgacacg attcgacgac cgtcccatct ttgatcgctc
     2401 aatatcgccc gtatgacctc gtagatattg tactgacgac acgttggttt cgcgagtagg
     2461 cggggttggc ggaatcaaga taaataagcg atagcaacaa tcatt
//
LOCUS       DATNDD010000052         4465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1268643, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000052 DATNDD010000000
VERSION     DATNDD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268643"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VN647_01165"
     CDS             complement(<1..247)
                     /locus_tag="VN647_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95663.1"
                     /translation="MPGLFDSHLKYVPESVVEKVLGRETRTAARHLIQRVSSSHGLNR
                     RGAFLVETQGEAMRPSGLFEYPGMKSKRRENRRMAKTA"
     gene            292..834
                     /locus_tag="VN647_01170"
     CDS             292..834
                     /locus_tag="VN647_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95664.1"
                     /translation="MQLFNPLIEVCLLSRLSRDGGLLLHAAGLVLDKQGYVFTGASGA
                     GKSTIAELFANQDAVVLSDERVILRRHGTAFFLHGTPWIGSGHYAENSSAPIYALFSI
                     SHGQEQHSFTPLVSSKTATLLLQQAFLPHWDRAGLEATLEFLASLTVQIPCHSLAFLN
                     RSDVVAFVRDHSPTLTPAFR"
     gene            831..1889
                     /locus_tag="VN647_01175"
     CDS             831..1889
                     /locus_tag="VN647_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWG95665.1"
                     /translation="MKQIDADTFLAELSSKARHRRRPESVTFELTYGCNLRCVHCFNP
                     THRALPQELSTSEVLRLIDQLAELGVLTATFTGGELTLRPDIAAILRHSQRKGLLIRV
                     LVNATRVTPTFVSLLRETGTEQICVSIYGATEQTYEQMTAVVGSHAAFLRGLTMLAEA
                     NLPVIVRMPVTTLNHHEVGACRSLAEHLGLRFQYCFDITPTVTGNMAPLAYRLPPERK
                     TAIDRAMVSGWGSEPIEEACGPTDRFIECACGHTRFAITPYGEMNFCTAFPIPRYDLR
                     NGTVSEGWELIKRTVDDARPNNRYECTTCEVRGYCRQGRNDAWLETGDMSVCLPHYKQ
                     WAELEHRTHALLHPRRPG"
     gene            1858..2817
                     /locus_tag="VN647_01180"
     CDS             1858..2817
                     /locus_tag="VN647_01180"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWG95666.1"
                     /translation="MPSFTLDDLVDAYTQVGTRNRVILEAFVLIAQRFRQQGIAFLVL
                     KGADVIPRLYGVRGARPISDVDLLVRESDLPAIDRLLTNLGFTRQIDGNPSYTSSERG
                     LSLDLITTIWYLDRQGLADVWSRAMMRPFGTTTICCLDTPDLLIYLTAYTVIHRGHLS
                     ASFVHDMKLLIEKESPDWPVVIARTQQAGLQAPLHHGLNHVRKIFPSIAVPETVLKLS
                     ALSNRNGQMLAWLFRKLVTTEPLAEIGHLLLFLTQPGLTKMIWLAQRLYPSPAFLSYR
                     YGSTERTRLWRVRLSRWYHLTKAGLILSGRVLRRLITASRPRS"
     gene            2814..3662
                     /locus_tag="VN647_01185"
     CDS             2814..3662
                     /locus_tag="VN647_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HWG95667.1"
                     /translation="MITRLFSPSTASARFSADQLPEAVHGSALWNVMTPVVNSKSMTP
                     TLQIGAELELEQPDNLQVGDVVVYRYDRQFICHRIHWIQGDRLFLRGDANTGSFEEVD
                     VRHVVGRVTCLVRRGKRIAVGPYTCVTVERVRSGSVWVRAPTWSVGQGRYRTLRFLNS
                     VASLPSVERIVRRILRKLMTIEIRERASLRSLEGYVVRQLVRLDRLAYSRRHLSLLNR
                     NGIVLVVRAGPLYLGTCTLNPWHLYMRPLLQSITTEVLSESIGVFLPTKTPHHSARPS
                     TAFGKQ"
     gene            3742..4395
                     /locus_tag="VN647_01190"
     CDS             3742..4395
                     /locus_tag="VN647_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWG95668.1"
                     /translation="MTGQRIITSITTINMLMNTSFQLAASIIDPKLVARVAKGEHHAL
                     SQLYDQSSTVLFSLALRILGNREEAADVLQEIYLDVWRKIVRYDVGRGTPIAWLIMLT
                     RSRAIDRLRTRGPRTFRQMSSSIDESLTSQVPGKSSGLFDSQADQELQYLIKEAWTNL
                     PQIQQQVVEMAYYDGLPDAEIAAQLNQPVETVKTCIALGMSQLRESLQSYCIQDEAV"
BASE COUNT          969 a         1268 c         1152 g         1076 t
ORIGIN      
        1 tggccgtttt ggccattcga cggttttctc tcctcttgga cttcatcccc ggatattcga
       61 atagacctga cggtcgcatc gcctctccct gagtttccac caggaaagct cctcttcgat
      121 tcagcccatg gctgctggaa actctttgaa tcagatgccg ggccgctgtt cgagtctctc
      181 gacccaaaac tttttcaacc acggattcgg gcacgtattt cagatgacta tcgaacagtc
      241 cgggcatgga tgctccctga tctggaggac ggccaagttg gatggagccc tatgcagcta
      301 ttcaatccgc tcatcgaagt ctgcctgttg tcgcgtttgt cacgcgacgg cggattgctc
      361 ttgcacgcag cgggcttggt tctcgacaaa cagggttacg tcttcaccgg ggcttcagga
      421 gccggaaaat caactatcgc agagttgttt gcaaaccaag acgccgtcgt tctcagcgac
      481 gaacgggtga tcttacggcg gcatggtacc gctttctttc tgcatggaac tccttggatt
      541 ggatccggac actatgccga gaactcatcc gctccgatct acgcgctgtt cagtatcagt
      601 catggacaag agcagcactc cttcactcct ttggtatcat caaaaaccgc cacactctta
      661 ctgcagcaag cctttcttcc ccattgggac cgcgccggtc tggaagcaac actagagttt
      721 ctggcatcac tcacggtgca gattccctgt cacagcctgg cttttctcaa ccgatccgat
      781 gtagttgcct tcgtgcgcga tcattcgccg actctcactc cggcttttcg atgaaacaga
      841 ttgatgccga cacattcttg gccgagctct cgtcgaaagc cagacatcgg cgccggccgg
      901 aaagcgtgac cttcgaactt acctatggtt gcaatctccg ctgtgtccat tgtttcaatc
      961 cgactcatcg cgccttgcct caggaactca gcacatcaga agtattgcgt ctcatcgatc
     1021 aattggccga actcggtgtg ctgaccgcca cattcacagg aggcgaactt actctcaggc
     1081 cagatattgc tgcgatcctc cggcactctc aaagaaaggg gctattgatt cgggtgctgg
     1141 tcaatgccac tcgagtgact ccgacctttg tatcgcttct ccgggaaacc ggcactgagc
     1201 agatttgcgt ctccatctat ggagcaacgg aacagacata cgaacagatg accgccgtgg
     1261 ttggatccca cgccgcattt ctccgtggac ttacgatgct ggctgaggcg aaccttcccg
     1321 tcatcgtccg catgccggtg accacgctca atcatcatga ggttggcgcc tgtcgcagtt
     1381 tggccgaaca tctcgggcta cgattccaat actgttttga catcactccg acggtgaccg
     1441 gcaatatggc gccgctagcg taccggctcc ctccggagag gaagactgcg atcgaccgag
     1501 caatggtctc gggatgggga tcggaaccga tcgaagaggc ctgtggtcca actgaccggt
     1561 tcatcgaatg cgcctgcggc cacactcgct ttgcgattac accctatggt gagatgaatt
     1621 tttgtacggc ctttcccatt cctcggtacg atctgagaaa cggaacagtc tcggaaggat
     1681 gggaattgat caaacggacg gtcgacgatg ctcgtccaaa caatcggtat gaatgtacga
     1741 cttgtgaggt tcgtggttac tgtcgccaag gccgaaacga tgcgtggctg gaaacaggcg
     1801 atatgagcgt ttgccttcct cactacaaac agtgggctga attggagcac cgtacccatg
     1861 ccctccttca ccctcgacga cctggttgat gcctataccc aggtcggtac cagaaaccgg
     1921 gtgatccttg aagcattcgt gctgatcgcc caacggtttc gacaacaagg gatcgccttc
     1981 cttgtattga agggtgcgga tgttatccct cgtctgtatg gcgtgcgggg agcacggccc
     2041 atttcagatg tcgacttatt agtacgtgaa tcggatttac ctgccatcga tcgactgctg
     2101 accaatctcg gcttcactcg acagatcgac ggtaatccgt catatacctc ctctgaacga
     2161 ggtctttcgc tggatctgat cacgaccatt tggtatctgg accgacaagg actggccgat
     2221 gtatggagcc gggcaatgat gcgaccgttc ggcaccacga ccatttgttg tctggatacg
     2281 cccgatctac tgatttatct gacggcttat accgtgatcc atcgaggaca cctgtccgca
     2341 tcgttcgtcc atgacatgaa actgcttatc gaaaaagaat cgcctgattg gcctgtagtc
     2401 attgcccgaa cccaacaggc cggactgcag gcgcccttgc accatggcct gaatcatgtt
     2461 cggaaaatct ttccctcgat cgccgttcct gagaccgtcc tcaagttgtc ggccctctcg
     2521 aacagaaacg ggcaaatgtt ggcctggctt tttcggaaac tagtgaccac ggagcctctg
     2581 gcggagatag gccatcttct gctgtttctg acgcaaccag gcttaacaaa gatgatctgg
     2641 ctggcacaac gcctctatcc ctcccctgca tttttgtcct accgttatgg ctcgacggag
     2701 cggaccagac tctggagagt gcgactgtct cgatggtatc acctgacaaa ggccggtctc
     2761 atcctgagtg ggcgagtgct caggcgactg ataacagcat cgaggcctcg ctcatgatca
     2821 ctcgactctt cagcccatcg accgcttccg cacgcttctc ggccgatcag ctacctgagg
     2881 cggttcacgg atcggcgctg tggaacgtca tgactcctgt cgtgaattcc aagagcatga
     2941 ccccgacgct tcagataggt gctgaactag aactagagca gccggacaat cttcaggtcg
     3001 gcgatgtcgt cgtgtaccga tatgatcgtc aatttatctg ccaccgcatt cactggatcc
     3061 aaggcgatcg gctttttctg cgaggggatg cgaatacggg ctcttttgag gaggtggacg
     3121 tacggcatgt tgtgggtcgg gtaacgtgtc tcgttcggcg cggaaaacga atcgcggtcg
     3181 gcccatatac ctgcgtaacg gttgaacgag taagaagcgg ttccgtatgg gtccgagccc
     3241 ctacgtggag tgtaggacag ggaagatatc gcaccttgcg gttcttgaat tctgttgcga
     3301 gtctgccgag cgtcgagcgc attgtccgtc ggatccttcg aaagctcatg accattgaga
     3361 ttagggagcg agcgtcactg cgttctctcg aagggtatgt tgtccgacag ctcgttcgtc
     3421 tcgaccgact cgcgtactct cggcggcatt tgtcactttt gaataggaac ggcattgtgc
     3481 ttgtcgtccg tgcaggccct ctctatctgg gaacctgcac gctcaatcct tggcacctat
     3541 acatgcgacc gctgctccaa agcataacca cggaagtcct ttctgaatcg atcggcgtat
     3601 tcctaccgac taaaactccg catcattccg cccggccctc aacagcattc ggaaagcaat
     3661 agtattttcg ttttctgtcg tgaaacactc ggcatcgttt tatgcgattt cttcgtcgag
     3721 tggatcgagg atgctatagt gatgaccggt caacggatca tcacctcgat caccacaatc
     3781 aacatgctta tgaatacatc ttttcagctc gccgcctcga tcattgatcc gaaactagtg
     3841 gcccgagttg ccaaaggcga acatcatgct ctgagccagc tctacgatca atccagtacc
     3901 gtattattca gtctggcatt acgcatcctc ggcaaccgcg aagaagcagc ggacgtactc
     3961 caggagatct acctcgacgt ttggagaaag atcgttcgct acgatgtcgg ccgaggcact
     4021 cctatcgcct ggctcattat gctgacccgc agccgggcta tcgaccgatt gcgaacacgt
     4081 ggccctcgta ccttccgcca gatgagctcg tccatcgatg aatccctgac tagccaagtt
     4141 cccggcaaaa gttccggtct ttttgattca caggccgacc aagagttgca atacctgatc
     4201 aaagaggctt ggacgaactt gccgcagata caacagcaag tggtggagat ggcctactat
     4261 gacggcttac ccgatgcgga gatcgcggcg cagcttaatc agccggtgga gacagtgaag
     4321 acctgcatcg cgctcggcat gtctcaactg cgcgaatcat tgcaatcata ttgcatacag
     4381 gatgaagcgg tatgacacat gacgaattag ctgccgccgt tcctttatac accgtcggag
     4441 cgctcgaacg gatagagcga caggc
//
LOCUS       DATNDD010000053         3100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2990850, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000053 DATNDD010000000
VERSION     DATNDD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2990850"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1289)
                     /locus_tag="VN647_01195"
     CDS             complement(261..1289)
                     /locus_tag="VN647_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HWG95669.1"
                     /translation="MRPGLLIAIGLCFISPGFGIAGTDAAVSGSKTITVSLDGTGDFS
                     SIQEAVDSAGKGDTVLIKAGAYDQDLTIHSKEKIKIVGEGVDKVVLLGRSELVGVLHV
                     GKWPYGATDIEISGLTIKEHGGHALGIFNGNRITLRQIHVKGMVFGQQVEDVRIEDCV
                     IGESETTGVHLSDSQVLLIGNLIHDNDHGVNVTGKSSVRLERNIITGNLFEAIVISDQ
                     AKAVLINNTLVKNGGGAAFLGLSTIEASGNILSLNKVGFLIAASSQTKTSYNALFSNE
                     VSYMRAGSPNIHAPELQAESDMTADPRFVDAEHDDFRLRPDSTLLNRGAFRYLGALPP
                     LLVPAQNR"
     gene            1354..1971
                     /gene="coaE"
                     /locus_tag="VN647_01200"
     CDS             1354..1971
                     /gene="coaE"
                     /locus_tag="VN647_01200"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWG95670.1"
                     /translation="MISSAAMILIGLTGGVATGKSTVAKMFKKCGAIVIDADELAREV
                     VRPGQPAWRDIMRRFGKSVLNSDRTINRQALGQIVFHDGTELRKLERIIHPRVALKQA
                     RLTRQASKTNPHVVVIYDVPLLFEAGIDKRVNKTVVVTSDRETQIRRLKKRNGFTRAE
                     ALRRIHSQMPLTMKLLRADYVLDGTKSRKSLSRDVRKLFEDLRSL"
     gene            2074..2997
                     /gene="trxB"
                     /locus_tag="VN647_01205"
     CDS             2074..2997
                     /gene="trxB"
                     /locus_tag="VN647_01205"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWG95671.1"
                     /translation="MHNIVIIGSGPAGLTAAIYAARANLAPLLVEGWQGGGQLTTTTE
                     VENYPGFAKGIMGPELMKEMRSQAERFGTVFKTGDATSADLQTRPFKVIVDGEETFET
                     KTLIIATGASPITLGLTNEKRLWGYGVSSCATCDGFFFKGKELVVVGGGDSAMEEATF
                     LTKFATKVSIIHRRDKLRASKIMQDRAMKNEKIAFIWNSVVEDVLGQDVVTGVRIRNV
                     ITGKVWDLHCAGFFLAIGHRPNTALFTGQLKMDGTGYILTNHGTATNVSGVFAAGDVQ
                     DSHYRQAITAAGTGCMAAMDAERFLETSSQG"
BASE COUNT          765 a          862 c          779 g          694 t
ORIGIN      
        1 ctcagacata ggcagcacgg ccccaaccaa cttgccggac tccatatcca tgagcgaggg
       61 aatgccgtca cctgagggct tttcgaggtt actgcgaaag ctagccacgg gagacctcct
      121 agttgaatcc ttctcggcgc gatgatgatg ttacttgaat tgaaggaatt gaccggaagg
      181 atgcgcgcta tagaggtagc ataccatgcg cattacccca catcgcgggt atacgctatc
      241 ccattgtttc aaatgagtta ttagcgattc tgtgcgggga ctaaaagcgg aggaagcgct
      301 ccaaggtaac gaaacgctcc gcgattgagc aacgtcgagt ctggtctcaa tcggaaatcg
      361 tcatgctcgg catctacaaa gcgcggatca gccgtcatat ccgactcagc ctggagttcc
      421 ggcgcatgaa tgttggggga tccggctctc atgtagctca cctcattact gaacagtgca
      481 ttgtacgagg tcttggtctg actcgaagct gcaatgagaa agccgacttt attgagactc
      541 agaatatttc ccgatgcctc aatcgtcgac aagcccagga atgccgcccc acccccattc
      601 ttgaccaggg tattattgat caagaccgct tttgcctggt cactgatcac gatcgcctcg
      661 aacaaatttc cggtgatgat attgcgctca agccgaaccg aggacttgcc ggttacattc
      721 acaccgtggt cgttatcatg gattaaattt ccaatcagaa ggacttgaga atcagacagg
      781 tgtacacctg tcgtctcact ctccccgatc acacagtctt cgattcgaac atcttctact
      841 tgttgaccga agaccattcc cttcacatga atctgacgca gcgtaatgcg gtttccgttg
      901 aaaataccga gagcatgccc tccatgctcc ttgatcgtga ggccgctgat ctcaatatcc
      961 gtcgccccat acggccattt ccccacatgc aacacgccga ccaactcact tcgccccagc
     1021 aagacgactt tgtcgactcc ctcaccgaca atcttgatct tctctttgct atgaatggtc
     1081 aaatcttgat cgtaggcccc ggccttgatc aacaccgtgt cgcccttccc ggcgctatcg
     1141 accgcctctt gaatcgaaga gaaatctccc gtgccatcca gcgagaccgt aatggtcttt
     1201 gaaccagaca cggcggcgtc ggtcccggca atgccgaacc cgggactgat gaaacagaga
     1261 ccgatcgcga tgagaagacc tggccgcatg gagaaattca tacaagcgaa aacccagggt
     1321 tgtcaatcgc gggtttctcg ccgaacctat tgcatgataa gctccgccgc catgattctg
     1381 atcggactca ccggaggcgt cgccactgga aaaagcaccg tggcgaagat gttcaagaaa
     1441 tgcggagcca tcgtgatcga tgccgatgaa ttggcgcgag aagttgtccg accaggccaa
     1501 ccggcgtggc gggatatcat gcgccgattc gggaagtccg tcttgaactc cgatagaacg
     1561 atcaaccggc aggccctcgg acagatcgtg tttcatgatg gaacagagct tcgaaagctg
     1621 gaacgaatca ttcaccctcg ggtggccctg aaacaggccc gacttaccag acaagcatcc
     1681 aaaacaaatc cgcatgtcgt cgtgatttac gatgtcccat tgctcttcga agccggcatc
     1741 gataagcggg tgaacaagac tgtcgttgtg acgtctgatc gcgaaaccca gatcaggcgg
     1801 ctgaaaaagc ggaacggctt cacccgagca gaggctctcc gacgaattca cagtcagatg
     1861 ccgctgacga tgaaactact tcgtgcagac tatgtccttg atggaactaa aagtcggaaa
     1921 agtctttcga gagatgtccg caaacttttt gaagacttac gctctctatg acaatctgtt
     1981 tcctttccgg cgaattctct ccatctgccc ttgccatgaa gctaaaatag cgttctacct
     2041 tctgcttcct tcgctgtgat acattcaatg accatgcata acattgttat catcggctcc
     2101 ggccctgctg gactcaccgc agccatctat gctgctcgtg cgaatctcgc ccccctcctc
     2161 gtcgaaggct ggcaaggggg cgggcagctc acgacgacca cggaagtcga aaactaccct
     2221 ggtttcgcaa agggcatcat ggggccggag ttaatgaaag agatgcgatc tcaagcagag
     2281 cggttcggaa cggtcttcaa aaccggtgac gccacatcgg ccgatctcca aactcggcct
     2341 tttaaagtca ttgtggatgg tgaagaaact tttgaaacca agacattgat cattgcgacc
     2401 ggcgcctctc cgatcactct tggtctcaca aacgaaaaac gattatgggg ctatggtgtt
     2461 tcgagctgcg cgacctgcga tgggttcttc tttaaaggca aagagctcgt ggtcgtaggc
     2521 ggcggagaca gtgcgatgga ggaagcgacg tttctcacca agttcgccac taaagtgtcg
     2581 ataattcatc gtcgtgacaa acttcgggct tccaaaatca tgcaagaccg ggccatgaaa
     2641 aatgaaaaaa ttgcattcat ctggaacagc gtcgtcgaag acgtgcttgg acaagacgtg
     2701 gtgaccggtg tccggatcag aaacgtcatt accggaaaag tgtgggattt gcactgtgcc
     2761 gggttctttc tggcgatcgg ccaccgcccg aacaccgcgc tcttcaccgg ccagctgaag
     2821 atggacggca ctggctatat cctgacgaat catggaacag ccaccaatgt ctctggtgtg
     2881 tttgcggccg gggatgtaca agactcccac taccgccaag ccatcaccgc agccggtacc
     2941 ggctgcatgg ctgcgatgga cgccgagcgg ttcttagaaa cttcaagcca aggttgaggc
     3001 tacggctgag cgttcacaga aagaaaggcc ttccacttta acctcgacat caacttatct
     3061 ccagtatgcg ttgtgtcatc gcccattacc acgagctcgc
//
LOCUS       DATNDD010000054         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_111428, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000054 DATNDD010000000
VERSION     DATNDD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111428"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VN647_01210"
     CDS             <1..887
                     /locus_tag="VN647_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWG95672.1"
                     /translation="MTVDCNSRYCLLNPYEGARLAVAEAARNLVCSGAVPIGLTDCLN
                     FGNPERPDIMWQFAMAIEGMKDACEHFQIPIVSGNVSFYNETNGLSIYPTPMLGMVGL
                     IDDTERSMTQWFKQEGDDVILLGSSREDLGGSEYLKVVHAREQGSPPYLSLNTEKALH
                     DCLLSLIRDGLLQSAHDCSEGGVAVALAESCISGPERTLGAVVRLTSGRLRKDAVLFG
                     ESQSRVVVSAKPVHRQPILDQARRFGVPVEVMGTVTGERLFVSVGNEGSAEPVIDQSA
                     AMLHDRWGFSLERALNQV"
     gene            909..2339
                     /gene="purF"
                     /locus_tag="VN647_01215"
     CDS             909..2339
                     /gene="purF"
                     /locus_tag="VN647_01215"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWG95673.1"
                     /translation="MNKELSIVSPDKFHDECAVFGAFGHEEAANLTYLGLYALQHRGQ
                     EASGIVAGDGDQFFIQKGMGLVADIFHKSVLEKLPGHMAIGHNRYSTTGGHDLKNVQP
                     LTANFALGNLALAHNGNLINAQMLRHELEAYGAIFQSTSDSEVIIHLIAHSRAGSFLA
                     RVVDALSQVRGAFSVVLMTDNGLIAARDPYGLRPLCIGRLRSSWIVASETCAFDLLDA
                     EYVREVEPGELIVISDQGLDSHHPFPKKNPAMCVFEYVYFARPDSRIFGANAVYATRK
                     ALGRQLAQESWVSADVVIPVPDSGVPAALGYSEGAGIRFETGLIRNHYVGRTFIEPEQ
                     SIRHFGVKVKLNAVPEVLNGKRVVVVDDSLVRGTTSRKIVKMIRQAGAKEVHMRISSP
                     PIVSPCFYGIDTPTKKELIASDHSTEEIRKYITADSLAYLSLDGMLKSAPRTADQYCT
                     ACFTERYPIPFTRAEELQLGLFESAR"
     gene            2360..2713
                     /locus_tag="VN647_01220"
     CDS             2360..2713
                     /locus_tag="VN647_01220"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG95674.1"
                     /translation="MTSPKEDNQKALQPRGRRRVQVDYPALFTGDERSGHGTVTNLTI
                     TGCEIESRIQFPIGAGLSLHVQASEARPPIIIALAIVRWKNGDRFGLEFVRFEGGTKE
                     QLEDMLNQRESPAED"
BASE COUNT          624 a          737 c          862 g          659 t
ORIGIN      
        1 cgatgacggt ggattgcaac agccgttatt gcctgttgaa tccctatgaa ggagcccggc
       61 tggccgtggc cgaggcggcg cggaatctcg tctgttccgg cgctgtaccg atcggcctga
      121 cggattgtct taacttcggc aatcctgagc gacccgatat catgtggcag tttgccatgg
      181 cgatcgaagg gatgaaagat gcctgcgagc attttcaaat ccccatcgtg agcgggaacg
      241 tcagctttta caacgaaacc aatggactct ccatttatcc gactcccatg ctgggcatgg
      301 tcgggctgat cgatgacact gaacggtcga tgactcagtg gtttaagcag gaaggcgacg
      361 acgttatctt gctgggctct tctcgcgagg atttgggtgg atcggaatat ctcaaagtcg
      421 ttcatgctcg tgaacaggga tcaccgccct atttgagcct gaatacggaa aaggctctgc
      481 acgattgcct gctgtcgctg attcgtgatg ggcttctgca gtccgcgcat gattgctcgg
      541 aaggtggtgt cgccgttgca ttggcggaaa gttgtatctc cggacccgaa cggaccctgg
      601 gtgctgtggt aagattgacc agcggtcgac ttcgaaaaga cgccgttctc ttcggcgaaa
      661 gtcagtcgag ggtggtggtt tctgcaaagc cggtccatcg acagcccatt ctcgatcagg
      721 cgaggcgttt cggcgtcccg gttgaagtga tggggaccgt taccggcgaa cggttgttcg
      781 tgtccgtggg aaatgagggt tcggcggaac ccgtgatcga tcaatcagcg gcgatgctgc
      841 acgatcggtg gggtttttct ttggagcgag cgctgaatca agtctagtct tgtgaattgc
      901 taaggatcat gaacaaagaa ctgtcgatcg tctctcccga taagtttcac gacgagtgtg
      961 ccgtcttcgg cgcattcggc catgaagaag ctgccaactt gacttatttg ggactgtacg
     1021 cgctgcagca tcgtgggcaa gaggcttcgg ggattgtcgc gggagacggg gaccaattct
     1081 tcatacagaa aggcatgggc cttgtcgccg atatctttca caagtccgtg ctggagaaac
     1141 tgccgggcca tatggccatc gggcacaatc gttactccac caccggtggc catgatctga
     1201 agaatgtcca gccattgacc gccaatttcg cgctcgggaa tttggccttg gctcacaacg
     1261 gcaatctcat caatgcccaa atgctccgac atgaactcga agcctacggg gcgatcttcc
     1321 agtccacatc ggatagcgag gtcattatcc acctcatcgc acactcgcgg gcaggatctt
     1381 ttctcgcgcg ggtcgtcgat gctctgagcc aagttcgagg tgcgttctcg gttgtgttga
     1441 tgacagacaa cggacttatc gccgcgcgtg atccctatgg gctcagaccg ctgtgcatcg
     1501 ggcgcctgcg cagcagctgg attgtggcgt ccgagacctg tgcgtttgac ttgctcgatg
     1561 ccgagtatgt tcgagaggtg gagccgggcg agttgatcgt catcagcgat caaggactcg
     1621 acagtcacca tccgtttccc aagaagaatc cggccatgtg tgtgttcgag tacgtctatt
     1681 ttgcgaggcc ggacagccgc atctttgggg ccaacgccgt ctatgccacg cgtaaggcat
     1741 tgggtcggca gttggcccaa gaatcatggg tgtcggcgga tgtcgtcatt cccgttcccg
     1801 actccggcgt gccggctgcg ctcggctatt ccgaaggggc gggaatccgg tttgaaaccg
     1861 ggttgatccg caaccactat gtcggacgga cgttcattga accggaacag tcgattcgtc
     1921 acttcggagt caaggtgaag ctgaatgccg ttcccgaagt cttgaatggg aaacgggtgg
     1981 tcgtcgttga tgattcgttg gttcgtggta cgacaagtcg taagatcgtc aagatgatcc
     2041 gacaagcagg ggcgaaagag gtccacatgc gcattagctc ccctccgatc gtctcgccct
     2101 gtttttacgg aatcgacaca ccgacgaaaa aagagcttat cgcctccgat cactcgacgg
     2161 aagaaatccg caagtacatc acggctgaca gtttggcgta tcttagcctt gacggcatgt
     2221 taaaatccgc cccaaggaca gccgatcagt actgtacggc ctgttttacc gagcgctatc
     2281 ctatcccgtt tacccgcgca gaagagcttc agttaggcct gttcgaatca gcgcgctgaa
     2341 ctggttctgc acggaagaca tgacgtctcc aaaggaagac aatcagaaag ccctgcagcc
     2401 acggggcagg cgccgagtgc aggttgacta tccggcttta ttcacgggag atgaacgatc
     2461 aggccatgga acggttacca atctgacgat taccgggtgt gagatcgaga gccggattca
     2521 attccccatc ggagccggtc tgagtctaca tgtgcaagct tcggaggctc gtcctccgat
     2581 tatcattgcc ctcgccatcg tccgttggaa gaatggagat cgattcggac tcgagttcgt
     2641 tcgctttgag ggtggaacca aagaacagct cgaggatatg ctgaatcagc gtgagagccc
     2701 tgccgaagat taacttcccc atgccttgcg gttgccgctc aaaaaaatgt catggtagga
     2761 tgcagcatcg gtcgatgctg cgcaggtgcg aagccttggt taccggatct ttggagagcg
     2821 gggaagtgaa caggtgagga ggcaaggaag cgatggatga gatggaggag gggaaacaaa
     2881 ag
//
LOCUS       DATNDD010000055         3232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2419492, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000055 DATNDD010000000
VERSION     DATNDD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419492"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1594)
                     /locus_tag="VN647_01225"
     CDS             complement(230..1594)
                     /locus_tag="VN647_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HWG95675.1"
                     /translation="MKAPASADRSRVLQRAASLPIAILLLMIAPFDTFGQVLDQQVNA
                     LLANNCAGLGNDGVTGFGSNLASICAFPTTTGSVSSGGGAASFQGSTVSILNRVLLGR
                     LEELRQEGQEEGRQPSSMQFNPLGILSMAGLGNLNVNSPFYAASTAGGSSNSFFTSNQ
                     NRWKGLGFFATGRVESLNRDITRFQDGYQSTILGFSAGVDYRVSKTVVAGMLGNFSNT
                     GGDWRGGGTFSTNSYGWLAFAHILPTERTFIQVTGGYTRNNYLVNRLATAVVTSTESN
                     DDRIVNSFASSNSNGDVANASVLAGYDHPFGRFIIGPRAGVYYTRTHIGSYAENGGGG
                     IGLTYGNQDIDSLQSTFGLFGSTAYSTPLGVLVHQVSADYVHEFMNHQRQIDVRFTED
                     LRANPTRFTFQNESPARDYGNISTGLVMVLPNGWQPFVNFRAMVGNDQFTNYAGTLGL
                     RIAL"
     gene            2031..3200
                     /locus_tag="VN647_01230"
     CDS             2031..3200
                     /locus_tag="VN647_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95676.1"
                     /translation="MSAMQLPPWLTSPGQYFLETNPQKRALEVLCMKLSLLSNLCSLI
                     VDEHERTHRSRGVIAPEHVLVRFPEHRVAILPTRWSASLSLAAGCDDTTFSVTNMSEE
                     MTRNLIAMPAGVNLVYAAPPVRDWPLGRKMSVTALVQSADPIPDDDQVMKGLVRVHVI
                     ADGLNSRDFSDRDVFRVSLPINGSRGACVELWARKVEAPERGIIMSGMTDVVSLDSWK
                     LFARTVGDVRSSAEVAVYRASSPAHDVYSCGMLLLRALLGSDESRWTRACEQLCSITE
                     GMEPIVQGVAEDDDYTIHLRVRDRLRESADGFEPGAVPEALWWDSLIAVLRACSRIQG
                     FSYASNAVLSEPNPARLLGRDLVSLARRARTEIFEARERDAIILRACDRALGHLG"
BASE COUNT          677 a          874 c          917 g          764 t
ORIGIN      
        1 acatctgcgg ggatagaagt ttgggggcag gtagtgatgc ctggatttca aggatccggt
       61 gacggtgcaa acgaactgag gatcgttatg attccactga cgagatacgg ttgcaacttg
      121 tggcgttccg atcagtattg cggtggccga tcaggaacgt ttcgcgagcc tccgaacgct
      181 ctcgacaaat atcctctcct cgatgtcgga tcgcctttgg ttccagtcgc tagagcgcga
      241 tgcgcaaccc cagtgtgccg gcgtagttgg taaattgatc attcccgacc atggcccgga
      301 aattcacaaa cggttgccag ccgtttggaa gcaccatgac cagcccggtc gaaatattgc
      361 cgtaatcgcg cgccgggctt tcgttctgaa aggtgaatct ggtcggattg gcccggagat
      421 cctcggtgaa gcgcacgtcg atttgtcgtt gatgattcat aaattcatgc acgtagtctg
      481 cgctcacttg atggacaagc acgccgagcg gagtgctgta cgctgtcgat ccgaacagac
      541 caaatgtgct ttggagcgaa tcgatatctt ggttgccata ggttaagccg atcccccctc
      601 cgccgttttc cgcataactc ccgatatgcg tgcgggtgta gtacacgccg gcgcgtggcc
      661 cgatgatgaa ccgtccgaat ggatggtcgt atccggcgag cacgctcgcg ttcgcgacgt
      721 ctccgttgga attactcgac gcgaaagaat tgacaatgcg atcgtcatta gattctgtcg
      781 atgtcacaac ggcggtcgcc agtcgattga ccagataatt gttccgggta tagcctccgg
      841 tcacctgaat aaaggtcctt tcggtcggca ggatatgcgc gaatgcgagc cagccgtatg
      901 aattggtgct gaacgtgcct ccgccgcgcc aatcgccgcc ggtgttcgaa aagttcccca
      961 acattccggc aacgacggtc ttactcaccc gataatccac gcccgccgaa aatccgagga
     1021 tggtcgattg atagccgtcc tggaaccggg tgatgtcacg attcagcgac tccactcgac
     1081 cggttgcaaa gaagccgagc cccttccatc ggttctggtt actcgtgaaa aaactgttgc
     1141 tggaccctcc agcggtggac gcagcataga agggcgagtt gacgttcaaa ttccccaggc
     1201 ccgccattga cagaatgccc aatgggttga attgcatgga cgacggttgc cggccttcct
     1261 cctgtccttc ctgtctcagt tcctccaagc ggcccagcag cacacgattc aagatagata
     1321 cggtagaacc ctgaaacgat gcggctccac caccggacga cacggatccg gtcgtcgttg
     1381 gaaacgcaca gatggaagcc agattggaac cgaaacccgt tacgccgtcg tttccgagcc
     1441 ctgcacagtt attggccagc aatgcattga cctgttgatc aagaacctgt ccaaaggtgt
     1501 caaacggggc gatcatcagc aacaggatgg caatcggcag tgacgccgca cgctgcaaga
     1561 ctctcgatcg atctgcgctt gcgggagctt tcatcggtgg cgttccttca tgttctcgtt
     1621 gttctctttt gagtaatcga attatagata cgtacgtagt tgagcgcaac gaaggataga
     1681 gtaaaaccgt cggcgacacc atgcgccttt ttgaggggag tatttgcagc tcgggcatcg
     1741 acagatggcg tggcaggatt tttacgaggc aattcgccgt cttgatttat cactgaagaa
     1801 cgcttaggct tgctctgctt ctctttctgc ctaggtgtgt ctacagtgct gactgacacc
     1861 aggctatcgc tgtgattgtg cttgggtaga acgagcgctg tctttccttg cacctgatga
     1921 tgcctggact tcaaggctcc gaactcgcgt ctaatcccgg tctcccttcg tttacatggg
     1981 aagacttctg tgaactcctc gacgactctt cgtcggagcg tcgtgcttcg atgtcggcaa
     2041 tgcagctccc cccatggctg acgtctcccg gccagtattt tctcgaaacc aatccgcaaa
     2101 aacgagcact agaagtctta tgtatgaaac tcagtttatt gagtaacttg tgctcactta
     2161 tcgtggatga acatgagcgt acccatcgtt cgcgaggtgt gatcgcccct gaacatgtcc
     2221 ttgtccggtt tccggaacat cgcgttgcca tattgccgac tcgctggagc gcctcgctgt
     2281 ctctggctgc aggttgtgac gatacgactt tctcggttac gaacatgtcg gaggaaatga
     2341 ctcgcaacct gatcgcgatg ccggccgggg tcaaccttgt gtatgccgcg ccgccggtga
     2401 gggactggcc gctcggaaga aaaatgtcgg tcacagccct cgttcaatcc gcggatccga
     2461 ttccggatga cgatcaggtc atgaagggat tggtccgagt ccatgtgatc gccgatggtc
     2521 tcaattcccg ggacttctcc gaccgtgacg tctttcgagt ctcgctcccg atcaacggaa
     2581 gccgtggtgc ctgcgtcgaa ctatgggcac gaaaagtcga ggcgccggag cgcgggatta
     2641 tcatgagcgg gatgacggac gttgtgtcgc tcgattcctg gaagttgttt gctcgtacgg
     2701 tgggagatgt ccgctcgtcc gcagaggtcg ccgtgtatcg agcgtcttca cctgcacatg
     2761 atgtctacag ttgtggcatg ttgctgttgc gggcgctgct cggatcggat gaaagccgtt
     2821 ggactcgtgc ttgtgaacaa ctctgctcga tcacagaggg aatggagccg atcgtccaag
     2881 gtgtcgccga agatgatgac tacaccatac atctgagagt ccgagaccgg ttgagagaat
     2941 cggctgatgg ctttgaacca ggagctgtac ccgaagcact ctggtgggat tcattgattg
     3001 ccgtgcttcg cgcctgctca cgcatacagg gattcagtta cgcgagtaac gcagttctgt
     3061 ccgagccgaa ccctgcacgg ttgttggggc gcgacctggt ctcgttggca agacgcgcgc
     3121 gcaccgagat attcgaggct cgcgagcggg atgccataat tctgcgcgca tgtgatcgag
     3181 cactgggcca ccttggctag cgacaacatg ccttgcaagc tgattatgcg ac
//
LOCUS       DATNDD010000056         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_413905, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000056 DATNDD010000000
VERSION     DATNDD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413905"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2233)
                     /gene="carB"
                     /locus_tag="VN647_01235"
     CDS             complement(<1..2233)
                     /gene="carB"
                     /locus_tag="VN647_01235"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWG95677.1"
                     /translation="MPKRTDIQSILMIGSGPIVIGQACEFDYSGTQACKALKAEGYKV
                     ILINSNPATIMTDPEMADRTYVEPITLDVLEKVIDRERPDALLPTMGGQTALNTTMGL
                     VKRGVLEKYGVSLIGASAEAIHKAEDREAFKQAMYRIGLRVPKSGTAHTRQDALTILD
                     TVGFPAIIRPSFTMGGTGGNIAYNREEFERLIDWALAMSPVTQVLIEESVIGWKEYEL
                     EVMRDLKDNVVIVCPIENFDPMGVHTGDSITVAPAMTLTDKEYQRMRDAALRIIREIG
                     VDTGGSNIQFGINPADGEMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     ITNDITGVTKASFEPAIDYVVVKIPRFAFQKFKGADPTLTTQMKSVGEVMAIGRTFKE
                     SLQKAIRSLELELNGLASRFGLDRGIPSEFNRPEAIEKLSRMLRIPLPERLWYVADAM
                     RLGFTDEELFATTKIDPWFLEQVRQLVTFERLLADHVADPAAVLGSELLWDAKELGFS
                     DDRIAQLLGCEAAAVQRARTQQGTRGVTYKRVDTCAAEFEAQTPYLYSTYGMECEARP
                     SDRQKVVILGGGPNRIGQGIEFDYCCVHAAMALREESIDTIMVNCNPETVSTDYDTSD
                     RLYFEPLTHEDVLNIVHREQPIGVVLQFGGQTPLKLALSLSKAGVRILGTSPDAIDLA
                     EDRERFRDLLNRLGLRQAESGIARSVEEAVQIAGRISYPVMVRPSYVLGGRSMQIVYD
                     ETGL"
BASE COUNT          474 a          680 c          600 g          548 t
ORIGIN      
        1 gcaagcctgt ttcgtcatac acgatctgca tcgaacgtcc gcccaacacg taggacggac
       61 ggaccatgac cggataactg atccgaccgg cgatctgcac ggcttcctcg acggatcggg
      121 cgattccact ttccgcttgc cttaagccca atctattgag gagatctcga aaccgttccc
      181 tgtcttccgc caaatcgatg gcatcgggac tggtgccaag aatcctcaca ccggctttcg
      241 agagcgaaag cgcaagtttc aagggcgtct gtccgccgaa ctgtaagacc actccgatcg
      301 gctgttcccg atggacgatg ttgaggacgt cctcatgcgt cagcggctcg aaatacagcc
      361 gatccgacgt atcgtaatcc gtgcttaccg tctccgggtt gcagttcacc atgatcgtgt
      421 cgatcgattc ttctcgaagc gccatcgccg catggacaca gcagtagtcg aactcgatcc
      481 cctgtccgat ccgattggga cctccgccga gaatcacgac cttttgtcga tccgacggtc
      541 tggcttcgca ctccatgccg tacgtggaat agagatacgg cgtctgcgcc tcgaactccg
      601 cggcgcatgt gtcgacgcgt ttgtaggtga caccccttgt tccttgttgg gtacgcgcgc
      661 gctgaaccgc tgccgcctcg catccgagta attgtgcgat ccgatcatca gaaaatccaa
      721 gttcttttgc gtcccagagc aactcgctgc ccaaaacagc tgcggggtcg gcgacatggt
      781 cggcaagcag tcgttcaaac gtcaccagct gtctgacttg ttctaaaaac cagggatcta
      841 ttttggtcgt cgcaaacagc tcctcgtccg tgaatcccag gcgcatggcg tcggccacgt
      901 accacagtcg ttccggtagt ggtattcgta gcatccggct gagtttttcg attgcttccg
      961 gccggttaaa ctcggatgga atgcctcgat caagtccgaa ccgggacgcc aacccattca
     1021 gttccaactc cagagagcga atggccttct gcagagactc cttgaaggtc cgtccgatcg
     1081 ccatcacttc cccgaccgac ttcatctgcg tggtcaaggt cggatcggcc cccttgaatt
     1141 tctgaaaggc aaatcttggg atcttgacca cgacatagtc gatcgccggt tcaaacgatg
     1201 cttttgtgac accggttatg tcattggtga tctcatccaa ggtgtatccg atggcgagtt
     1261 tggcggcgat cttcgcgatc ggaaatccgg tcgcctttga cgccaatgcc gagcttcgag
     1321 atacccgagg attcatttca atgacaacca tctctccgtc ggcagggttg atgccgaatt
     1381 gaatatttga ccctcccgta tcgactccga tctctcggat gatacgaagt gccgcgtccc
     1441 gcattcgctg gtattctttg tcggtcaacg tcatggccgg cgcgaccgtg atactgtctc
     1501 ctgtgtgtac gcccatggga tcgaagtttt caatcggaca cacgatgacg acgttgtcct
     1561 tcagatcccg catgacctcc aattcgtact ctttccaccc aatgaccgat tcttcgatca
     1621 acacctgagt gaccggactc atggccagag cccaatcgat cagcctctcg aattcttcac
     1681 ggttataggc gatgttcccg ccggttccac ccatcgtgaa ggatggacga atgatcgcgg
     1741 ggaagccgac ggtatcgaga atggtcagtg cgtcctgtcg cgtatgcgcc gtaccgctct
     1801 tcggcacccg caggccgatt cgatacatcg cttgcttgaa cgcttcacgg tcctccgctt
     1861 tatgaatggc ttccgctgaa gcgccgatga gcgaaacacc gtatttctcg aggactcctc
     1921 gcttgaccaa ccccatagtg gtattgaggg ccgtttgccc acccatggtc ggaagcaagg
     1981 catccgggcg ctcccgatcg atcaccttct ccagcacatc taaggtaatc ggttcgacat
     2041 atgttcgatc ggccatctcc ggatccgtca tgatcgtggc cggattgctg ttgatgagga
     2101 tgactttata accctccgct ttcagcgctt tgcaggcctg cgtgccggaa taatcgaact
     2161 cacaggcttg gccgatgacg atcggtccag aaccgatcat gagaattgac tgaatgtccg
     2221 tccgtttagg catggtgtct taatagtcgg attaactgga tgggagatcg ggcggcccaa
     2281 tcagcggatg atacggagcc gg
//
LOCUS       DATNDD010000057         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_691014, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000057 DATNDD010000000
VERSION     DATNDD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691014"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1735)
                     /gene="yidC"
                     /locus_tag="VN647_01240"
     CDS             complement(68..1735)
                     /gene="yidC"
                     /locus_tag="VN647_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWG95678.1"
                     /translation="MEKRVVIFLVLSLAVILGYDLLLKQLGLLPSSETVQEPPAATTG
                     TTAGGSEVAPAAPATDRPVSSGKHEEASNQPSSSDQAQPHEQIVMIETDLVRVGLDAR
                     GGVISSWELKRLRTAPPEDKPVQLVHQGAKFKGPLSVSTSDAVADKVLREGLYVIETD
                     FTSLDSAHPVGHATLRFRDTTTGLTVVKRLTFHQGSYLVDVSLTVEGLTGAYDVVLGT
                     NFGIVEWGDGFIGLIGSASLVDGKVEKETPDNELERKGAVQWVALQDKYFLSVLMPQG
                     ASAALAKKEGEKLVSAGVRMPPPSGGGTTTLQLYAGPKEYDTLRSLNVGLEETIDFGW
                     FIFGSWSVVKAVAKPIFYVLRFINEYTHNYGVTIILLTMGIKMLFVPLQFKSYKSMKQ
                     MQMIQPKVLALQEKYKDDRDKVNKELIKLYRDHKVNPVGGCLPMVLQMPVFVALFNIL
                     YMTIDLRQAPFFGWITDLSVQDPYYVLPIIMGATMVIQQKITPTTMDPTQAKIMLVLP
                     VFMTFLFINFPAGLVLYWLTNNMLTIGQQVLTDRLYANRPFIIDPAK"
     gene            complement(1735..1977)
                     /gene="yidD"
                     /locus_tag="VN647_01245"
     CDS             complement(1735..1977)
                     /gene="yidD"
                     /locus_tag="VN647_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWG95679.1"
                     /translation="MRTMCVAVITAYRFLISPWLGPACRFEPTCSEYACQAIDRFGVV
                     KGCGLTIWRLLRCHPFHRGGYDPLLDETQRQGVKHL"
     gene            complement(1977..2342)
                     /gene="rnpA"
                     /locus_tag="VN647_01250"
     CDS             complement(1977..2342)
                     /gene="rnpA"
                     /locus_tag="VN647_01250"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWG95680.1"
                     /translation="MSQGRGDRSFFLRASRDIEHVKQHGRRLSTACFNLLICRTDAQT
                     TRVGIVVGKRFGIAVRRNRAKRLFRELSRQVRGELAADHALLVFPKREALTLSFEKLK
                     HLWWSTLRRQGVLNSPEKS"
     gene            complement(2485..2622)
                     /gene="rpmH"
                     /locus_tag="VN647_01255"
     CDS             complement(2485..2622)
                     /gene="rpmH"
                     /locus_tag="VN647_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWG95681.1"
                     /translation="MSFTFQQPSNLKKKREHGFRKRMSTKNGRRVLARRRAKGRARLT
                     V"
BASE COUNT          625 a          848 c          800 g          621 t
ORIGIN      
        1 caccggccgg ggtggcgatc gcacagatgg tgtcctcgag cgctccgctc atcatgagcg
       61 ccatctctca cttggcggga tcgataatga acggccggtt ggcatacagc cggtcggtca
      121 gcacctgttg cccaatggtc agcatattgt tggttagcca atacaacaca aggcctgccg
      181 gaaaattgat gaagagaaat gtcatgaaca ccggcaggac caacatgatt ttcgcttgag
      241 tcggatccat ggtggtcggg gtgatttttt gctgaataac catcgtcgcc cccatgatga
      301 ttggaagcac ataatagggg tcttggaccg acagatccgt aatccagccg aaaaaaggcg
      361 cctgccgcag atcgatggtc atgtagagaa tgttgaacag cgccacgaac acgggcattt
      421 gcagaaccat cggcaggcag ccacccaccg ggttcacttt gtggtcacga tacagcttga
      481 tgagttcctt gttcactttg tcgcggtcgt ctttgtactt ttcctgcaac gccagcactt
      541 tgggctggat catttgcatc tgtttcatgg acttgtagct tttgaactgc aggggcacga
      601 agagcatctt gatgcccatc gtcaagagga taatcgtgac gccatagtta tgcgtgtact
      661 cattgataaa gcgaagcaca tagaagattg gcttggcgac ggctttgacg acgctccagc
      721 tgccgaaaat gaaccatcca aaatcgatgg tttcctcaag ccccacgttc aacgagcgca
      781 gcgtgtcgta ttccttcggg cccgcatata gttgcagcgt cgtcgttcct ccgccggaag
      841 ggggcggcat tcggactccg gcggacacga gtttttctcc ttcctttttg gccaatgccg
      901 cactggcgcc ctggggcatt aagaccgaaa ggaaatactt gtcctgaagg gccacccatt
      961 gaaccgcccc tttgcgctcc agttcgttgt ccggcgtttc cttttcgact tttccgtcca
     1021 caagagaggc tgaaccgatc aacccgataa aaccgtcgcc ccactcgacg atgccgaagt
     1081 tggtacccag gaccacgtcg tacgcgcccg tcaaaccctc gacggtcagg ctcacatcca
     1141 ccaggtagct tccttggtga aaggtcaggc gcttcaccac ggtgagcccg gtggtcgtat
     1201 cccggaatcg cagggtcgca tgaccgacgg gatgggcgct atcgaggctc gtaaaatcag
     1261 tttcgatcac atagaggccc tctcgaagca ctttgtcagc gaccgcgtcg gatgtactga
     1321 ccgacagagg gcctttgaac tttgccccct gatggacgag ttgcacgggc ttatcttccg
     1381 ggggagctgt gcgaagacgc ttgagttccc agctggagat cacgccgcca cgggcatcca
     1441 accccactcg aaccagatcc gtctcgatca tgacgatttg ctcgtgaggt tgggcttgat
     1501 ccgaagagga aggctggttg gacgcttcct catgctttcc cgaggaaacg ggacgatctg
     1561 ttgcgggcgc ggccggagcc acctcggatc caccggccgt ggtacccgtc gtcgcagcgg
     1621 gcggctcctg cactgtttcg gacgacggta gaagtcccaa ctgcttcaac agcaagtcat
     1681 agccgaggat tactgccagg gacaggacga gaaaaataac aacgcgcttt tccatcacag
     1741 atgcttgacc ccctgccgct gcgtctcatc gagtaaggga tcgtatcctc cccgatgaaa
     1801 gggatgacat ctcagtaaac gccagattgt cagaccgcac cccttgacca cgccgaagcg
     1861 gtcgatcgcc tggcacgcgt actcagagca ggtcggctcg aaccggcaag ccgggccgag
     1921 ccaaggagag atcaagaacc gatacgccgt aatgactgcc acgcacatcg ttctcatcac
     1981 gatttctccg ggctgttgag gactccttga cgccgcagag tcgaccacca caggtgtttc
     2041 agtttttcga acgaaagggt gagggcttcc cgcttcggaa acacgagcag cgcgtggtca
     2101 gcggccaact cacctcggac ctgccgcgac aactcgcgga agagacgttt cgcacgattc
     2161 cggcggacgg cgatgccaaa ccgtttgccg acgacaatgc ccactcgagt cgtctgtgcg
     2221 tcagtcctgc agattaataa gttaaagcag gcagtcgaca gccgacggcc atgctgcttc
     2281 acatgctcga tgtcgcggct cgcccgaaga aagaaggacc gatcacctct gccttgcgac
     2341 atcgtccggc tcattcacgg ccgcgccgaa cccgtccgcc cgactcctgc gttccgggtt
     2401 gggacggacc gctccgtgtt gctcttggaa aacgtccctg cttgaggaag acaagggggg
     2461 cggaagcccg tgcagcgcga gtatttagac ggtcaaccgc gcgcgaccct tggctctacg
     2521 gcgggccaag actctgcggc cgttctttgt gctcatgcgt ttgcggaaac cgtgttctcg
     2581 cttctttttg aggttcgacg gctgttgaaa cgtaaacgac atacgagtca ccctttcgag
     2641 gagaatctca aatgaacgtc gcattatagg ggcgccccca ccacctgtca aaccaagcgg
     2701 cacgtgcaca ttctgtcccc gctcctcgtt gataagctgg tccgctcaag ttccgatcgt
     2761 cgttcagatt gaggcggttt tgtggacctt tcccccaaaa tggactagac tgcctcagac
     2821 tttgataccc gtagatgtgt gggcgtcgcc ataacccatt acggaatcaa cagttccgaa
     2881 ccgggcgctt cgaa
//
LOCUS       DATNDD010000058         3530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3285271, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000058 DATNDD010000000
VERSION     DATNDD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3285271"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VN647_01260"
     CDS             <1..899
                     /locus_tag="VN647_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWG95682.1"
                     /translation="EEAERRRVLYVGMTRAKELLLLSGGTTTRSVGETVFDLLGNIGT
                     GDIGTASTEALKVGGSEIPHRVIRAPERKRPRRRVDRMANAALIDPKEIAERWEIRTA
                     RWTEIRETPHHLTPTTLGKRTASVIREPIPVRQDAEIGRLAGVVAHRLLERWDFSHDP
                     SELFVQVVLAVQATLGPDDQSRAGAVVESVHDLLATFSRSEAYARLRSSKILGREVPF
                     IMPWEDRRVMEGVIDLIYRLNGELWIADYKTDAISADEAVLRAERYRMQSHVYKAAVK
                     QSLRIEPRFHCLFLRCAMTVEL"
     gene            918..1295
                     /locus_tag="VN647_01265"
     CDS             918..1295
                     /locus_tag="VN647_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWG95683.1"
                     /translation="MAKRDHISSEAFAQLVQQAIADLPPPYAKLMESIAVVVEEEPPE
                     DVLEDLDLDSEDDLLGLYQGRSLAADSFFGSGGEPPPQISIYRGPILRLCESPEEVVQ
                     EVYDTVVHELGHHVGLDDDEMPY"
     gene            complement(1408..2220)
                     /gene="lgt"
                     /locus_tag="VN647_01270"
     CDS             complement(1408..2220)
                     /gene="lgt"
                     /locus_tag="VN647_01270"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWG95684.1"
                     /translation="MGPPAAIPYPNIDPVLVALGPIQLRWYGLMYLIGLTMAYFLIQH
                     KVTRKGLAIRRDQIYDMVVYAAFGVFLGGRIGYTLFYNFSYYSQNPLKLLAVWEGGMS
                     FHGGLLGTIIALIWFSRRQGIPAYTIADLAACVTPIGLGFGRIGNFINGELFGRSTDV
                     DWCMVFPAGGPACRHPSQLYEATLEGLVLFTTLWWIDRRPTPPGTVFWSFITGYGISR
                     LIVELFREPDQHLGFILGPITMGQILSLPMVLIGTAMLVWGYQRERQKVAHS"
     gene            2665..2934
                     /locus_tag="VN647_01275"
     CDS             2665..2934
                     /locus_tag="VN647_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWG95685.1"
                     /translation="MSEQGNQVAKAAAYIAGGAVIGAGLGLLFAPQTGVETRRSIGRY
                     ARKAQVQTSRWGKAIKSGVENALDRESVKEKSCEAERPQLTAVLN"
     gene            complement(2999..3136)
                     /locus_tag="VN647_01280"
     CDS             complement(2999..3136)
                     /locus_tag="VN647_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95686.1"
                     /translation="MSSTLVPWHFLNPKGIPDISGYPREGAQVGELAGTISIQIPSDH
                     R"
BASE COUNT          839 a          902 c         1023 g          766 t
ORIGIN      
        1 gagaagaggc ggaacggcga cgtgtgctct atgtggggat gaccagagcc aaagagttgt
       61 tgctattgtc tggggggacc accactcgct ccgtcggtga aacggttttc gacctgctgg
      121 ggaatatcgg tacgggggac atcgggacag cctcgaccga ggccttgaag gtcggcggca
      181 gcgagattcc tcatcgagtg atcagagcac cggaacgaaa gcggcctcgc cgacgagtgg
      241 acagaatggc caatgcggca ttgattgatc cgaaagagat tgccgagcgg tgggaaataa
      301 ggaccgctcg atggacggag attcgtgaga ccccgcatca tctgacgccc acgacgctgg
      361 gcaaacgaac ggcgtccgtc atacgtgagc cgattccggt tcgacaggat gcggagattg
      421 gacggttagc tggtgtagtg gctcatcgtc tgcttgaacg atgggacttt tcacatgatc
      481 cttccgaatt atttgttcag gttgtcttag ccgttcaggc aactcttgga ccggatgacc
      541 agtctcgtgc cggagcggtc gttgaatccg tgcacgatct ccttgcaact ttcagtcgat
      601 cggaagccta tgcacgcctc cggtcatcca agatccttgg ccgtgaagtt ccgttcatca
      661 tgccgtggga agatcggcgg gtcatggaag gagtcatcga cctcatctac cggctgaatg
      721 gagagctgtg gatcgccgac tataagacag atgctatctc ggccgatgaa gcagtgcttc
      781 gtgcagagcg gtaccgaatg caaagccatg tgtacaaggc cgctgtaaaa cagagcctaa
      841 gaattgagcc gcggtttcac tgtctgttcc ttcgttgtgc gatgacggta gaattataag
      901 ggtaggcgat aatggagatg gctaagcgag accatatctc ctctgaagcc tttgcacagc
      961 tggttcaaca ggcgatcgct gatctgcctc ccccttatgc caagctgatg gaatcgatcg
     1021 ccgtcgtggt ggaggaagaa ccgccggaag acgtgctcga ggaccttgat ctcgacagtg
     1081 aggacgactt gctcggcctg tatcagggcc ggtctctggc ggctgattcg ttcttcgggt
     1141 cgggaggaga accaccgcct cagatttcca tctatcgggg gccgatcctc cgattgtgtg
     1201 agagtccgga ggaggtggtg caggaagtgt acgataccgt cgttcacgaa ttgggccacc
     1261 atgtcggcct ggacgatgac gagatgccgt actaaattca gtgctgaaaa tcgagtgctc
     1321 gcctcgctgg gtcgaagcgg gctgtgtgct gagttgaaaa acgcccggaa actccgctca
     1381 gcactcggga ttcagcgctc atcactgtca agagtgcgcg accttctgtc tttctcgttg
     1441 gtagccccag acaagcatgg ctgttccgat caacaccatg ggcaaggaga gaatttgacc
     1501 catggtgatc ggtcccaaga taaatcccaa gtgctgatcc ggttcacgga agagttcaac
     1561 gatcagtcgg ctgattccgt aaccggtgat gaaactccaa aagaccgtgc cgggcggggt
     1621 cggacgtcga tcgatccacc ataaagttgt gaataagaca agaccttcca gcgtggcctc
     1681 atacaattgc gagggatggc ggcaagcagg ccctcccgct ggaaagacca tacaccagtc
     1741 cacgtcggtt gaccgaccga agagttcacc gttgatgaag ttcccgatcc gaccgaaccc
     1801 taagccgatc ggcgtgacgc aggcagccaa atcggcgatg gtataggcag gaattccttg
     1861 ccgacgactg aaccagatca gcgcaatgat agttccaagg agcccgccgt ggaaggacat
     1921 gcctccttcc cagactgcaa ggagtttcag cgggttttgg ctgtagtagg aaaagttgta
     1981 aaagagggtg taaccgatcc gtccaccgag aaacacgccg aaggcagcgt agacgaccat
     2041 gtcgtagatc tggtcccttc tgatcgccag ccccttacgg gtaactttgt gttgaataag
     2101 gaagtaggcc atggttaggc cgatcaggta catcagccca taccagcgga gctggatggg
     2161 gcccagcgcc acaagaaccg ggtcgatgtt ggggtagggg atggcggctg ggggacccat
     2221 tactgtgctt tcagcatcgt ccttcgcgag atggcattca taacgtgaag ggaacacgaa
     2281 tcttgaatca tacgcagtac tcgttgcgac ctgcggcaaa acttgccgca ggatcatacg
     2341 ggggctcacc tgtcaatgca agcgcgccaa aggttagaaa cggttaaagt ttgaccacgg
     2401 caaatcggct tccatggctg attcaccagc acgtatgagt cgaaagtgtt ggctttctga
     2461 aacgactgtg tctcagtctg tcgagaaaaa gagaccaaaa attagtcact cttaattgcc
     2521 aaaaaacgcc ccctacggtt tttcgcggga atcgtctctc ctacgaatat tcttgtcttg
     2581 gcacagagca tgctcacaga cctcggcata ggaagagttt ttaaacagtc ttccggctca
     2641 ttcaccatca tgaggaggtt caccatgtcg gagcagggga atcaggtcgc caaggcagca
     2701 gcgtatatcg ccggtggggc ggtcatcgga gcgggactcg ggctcctctt cgctccacag
     2761 accggtgtgg aaactcgtcg aagtatcggc cgctatgccc gcaaggcgca ggtgcagacc
     2821 agtcgatggg gcaaggcgat caaatcggga gtggagaacg cgctggaccg cgaatccgta
     2881 aaagagaaga gttgtgaagc agaacgaccg caactcactg cggtgttgaa ctaagcttct
     2941 cgaagatcat cggtagctga tcaccccgtc gcgctcctcc gtcggcacgg gctgactgct
     3001 accgatggtc cgaggggatt tgaatactga tggttccggc cagttctccc acttgggcac
     3061 cctccctcgg atagccggaa atgtccggga tccctttggg attcaagaag tgccatggca
     3121 ccagcgtgct tgacacaatc ccctttcctc tatgcttcta ttgttacacc gaaccgcatc
     3181 ctgtagctgg cgtttgctgt actccgatat ttcttcacca gaatagctct tgaacctcca
     3241 aggcataggc ccgccttcta tcctttgatc cgttgctgct tctttaggtc tgaccgggat
     3301 caaggtctga ccgggatcac atcttacgat ggaaaggatg gcatggagac ccagtgaaat
     3361 accaccggca taacataaag ctgtgtatag aaattcgatg gaattacctc agaggatttt
     3421 agggatactc ctggcttgga agaagacaat ttgaggctat gctgacaaat atgagaataa
     3481 accatagaaa aggtgtccct atagtggagc gcatagctgc catggaagag
//
LOCUS       DATNDD010000059         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2175468, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000059 DATNDD010000000
VERSION     DATNDD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175468"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..299)
                     /locus_tag="VN647_01285"
     CDS             complement(3..299)
                     /locus_tag="VN647_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95687.1"
                     /translation="MDDDVKSLVQQYIETRKQQRNDLMQELGLSEEEADQAVSTLELY
                     YHIEASENQWTQAWKAAKRDFLADGERTGIRLADLVVKYVDIARLGGGPESRWT"
     gene            complement(375..725)
                     /locus_tag="VN647_01290"
     CDS             complement(375..725)
                     /locus_tag="VN647_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95688.1"
                     /translation="MTLERMSDEEILHIANPIMDNLMDASTAIDHARHIRDFSDRMKT
                     LVTKEYLQKVCEKYQGEKGYFSGREPVAVFKRPGAAAIIWKQWFTKAPGEFVAEMLLV
                     EKDGTFLVDHVMVF"
     gene            complement(872..1264)
                     /locus_tag="VN647_01295"
     CDS             complement(872..1264)
                     /locus_tag="VN647_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWG95689.1"
                     /translation="MPLSPKQISDAINRYFAAWSSLDPSAYVACFDEHAVVHDPYGST
                     PHKGASALREFFGGIAHAIQDVSIQADSVYAASNRAAVVFQGKAIGKNLKPVEVAGID
                     VFEFNEAGRITALWAYWDPAAVLAKLRQ"
     gene            1480..>2743
                     /gene="pyk"
                     /locus_tag="VN647_01300"
     CDS             1480..>2743
                     /gene="pyk"
                     /locus_tag="VN647_01300"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWG95690.1"
                     /translation="MQKTRIICTIGPATESYEMLQKMYEAGMSIARLNMSHGDHDSHA
                     KVIQHIKTLNKKVKFPIPILLDTQGPAIRTGDLSNDLDLRQGAIVSVTTRGPMDVEES
                     SIHIDYADLLEQVNVGDKITVDNGLINLEVLEKEERHMRCRVVDGGLLKSKRHVNLPG
                     IRVNLPAITQKDTKDILFGLERDVDFIALSFVREAADIQQLKALMGEKVGRVKIVAKI
                     EDQEGVRNLEEIIRESDGIMVARGDLGVEINIEDLPNVQRTIVQRCAEHGKRVIVATH
                     LLESMIHNPHPTRAEVTDVANAIYEEADAVMLSGETTVGKYPVKCVEYLRKIALKSET
                     IPGLRFAKQLRDTGNKQQLAAAAVQLAEGVSAKGIVVITRRGLMADLVANCRPFSTNI
                     YAFTNMSQPRRTMMLNRGVFPFKIDFSSD"
BASE COUNT          634 a          783 c          739 g          587 t
ORIGIN      
        1 gcctacgtcc atcgactttc tggtcctcca cccagacgtg cgatgtcgac atatttcacg
       61 acaaggtcag ccagtcgtat tccagtacgt tccccgtcag ccagaaaatc gcgcttggcc
      121 gccttccacg cctgtgtcca ctgattctca gatgcctcga tgtggtaata cagctcaagc
      181 gtcgaaacgg cctggtccgc ctcctcctcg gagagtccca actcctgcat gaggtcgttt
      241 ctctgctgtt tccttgtctc aatgtactgt tgcaccaggg atttcacgtc atcgtccatc
      301 gctgtgctcc tcgcctatat caccgctgat cgtctatcag gtccccagcg cccatcagag
      361 agccctccac cgtcttagaa caccatcacg tgatcgacga gaaatgtacc atctttttcg
      421 acgaggagca tttcggcaac gaattcacct ggcgcttttg tgaaccactg cttccagata
      481 atcgccgctg cgccaggacg tttgaacacg gcgacaggtt cccgccccga gaaatagcct
      541 ttctctccct gatatttctc acagaccttc tgaaggtact ccttggtcac gagcgtcttc
      601 attcggtcgc tgaaatctcg aatgtgacgc gcatgatcga tcgcagtcga tgcatccatc
      661 aaattgtcca tgatcggatt cgcgatgtgg aggatttcct cgtccgacat gcgttccaag
      721 gtcatttgcc tctcctgggt gtgataccga acgccgcaga ctacccgaca tttcaaccaa
      781 gaacgatgtg tggagcaagt aggtctcagg aaccgaaaag agacaacgta gaggatacgg
      841 atgccagaaa gaagcgtcta gttgcgagat tctactgccg cagcttggcc agaaccgctg
      901 cagggtccca gtaggcccat aacgccgtga tccggcccgc ttcattgaat tcgaagacgt
      961 cgatgccggc cacctccact ggcttcagat tcttcccgat ggccttgcct tgaaagacga
     1021 ccgccgcacg attgctcgcc gcatacaccg aatccgcttg aatgctgacg tcctgaatcg
     1081 catgcgcaat gccaccaaag aattcccgta acgcgctcgc gcccttgtgc ggtgtgctcc
     1141 cgtatggatc atgcacaacc gcatgctcat cgaaacaggc cacatatgca gagggatcaa
     1201 ggctggacca ggccgcgaag tagcggttga tggcatcgga tatctgtttt ggagaaagag
     1261 gcatggtctt aggagacgat cttagtccgt gtgccgcagt ttcgcaacgc gccatggtcg
     1321 aagtaaagag gtcaggagtg ttttttcgac acaccatgga cctggccgac gataaaaagt
     1381 agaatagtcc cggttctgtc cgtatcctgc cgtatcacgt cgtcatcaga ttggcttcac
     1441 ctcggcgagt gggaagaggc cacttcaagg agagcacgca tgcaaaagac ccgcattatc
     1501 tgcacgatcg gccctgccac ggaatcgtac gagatgttgc agaaaatgta cgaggccggg
     1561 atgagcatcg cgcgactgaa tatgtcccac ggcgaccacg actcccacgc caaggtcatt
     1621 cagcacatta agacactcaa caagaaggtc aagtttccca tcccgatcct cctggatacg
     1681 caaggtccgg cgattcgcac cggggacctc tccaacgacc tcgatctgcg gcaaggcgcc
     1741 attgtctccg tcaccacgcg cgggccgatg gacgttgagg aaagctctat ccacatcgac
     1801 tatgcggact tgcttgaaca ggtcaacgtc ggggacaaga tcactgtgga taacgggctg
     1861 atcaacctgg aggtgttgga gaaggaagag cgccacatgc ggtgccgtgt cgtggacggt
     1921 gggctcttga agagcaagcg ccacgtcaat ctcccgggaa tccgtgtcaa tctgccggcg
     1981 attacgcaaa aggacaccaa ggatattctg ttcggcctgg aacgggatgt ggacttcatc
     2041 gcgctttcgt tcgttcgtga agccgcggat attcagcaac tcaaggcact gatgggtgaa
     2101 aaggtcggga gagtgaagat tgtcgcaaag atcgaggacc aggaaggcgt gcgaaacctg
     2161 gaggagatca tcagggaatc ggatggcatt atggtcgcgc gtggcgatct gggggtggag
     2221 atcaacatcg aggatttgcc gaatgtccag cgcacgatcg tccaacgttg tgccgagcat
     2281 ggaaaacgcg tgatcgttgc cactcatctg ctcgaatcca tgatccacaa tccgcacccc
     2341 acccgcgccg aggtgaccga cgtcgccaac gcgatctatg aagaggccga tgccgtcatg
     2401 ctgtccggag aaaccacggt tgggaagtat ccggtgaaat gtgtcgaata cctgcgcaag
     2461 attgcgctga aatccgagac gatacccgga ttgcggtttg cgaaacaact gcgcgatacc
     2521 ggcaataaac aacaattggc ggcggcggcc gttcagctcg ccgagggggt cagcgccaag
     2581 ggtatcgtcg tgatcacgag acggggactc atggccgatc tcgtcgccaa ctgccgtccg
     2641 ttctcgacca acatctatgc gtttaccaac atgagccaac cgcgacggac catgatgctc
     2701 aaccgtggtg tctttccgtt caagattgat ttcagttcgg acc
//
LOCUS       DATNDD010000060         3230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1097570, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000060 DATNDD010000000
VERSION     DATNDD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097570"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="hisZ"
                     /locus_tag="VN647_01305"
     CDS             complement(<1..1075)
                     /gene="hisZ"
                     /locus_tag="VN647_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWG95691.1"
                     /translation="MATILPEAARHVRHLEAGLLAYFNRFGYDEIILPTFEYLDVLAP
                     GLEPTLLENSYKIVDRTTGRILLLRPDVTAQIARTVAMGMVGAHFPLRLSYRATVFRH
                     EPEHAGRGREIFQVGAELIGADDPSADSEIIMLLIECLRQVGLRSFKISLGHVGFFKG
                     LLVRAGLSPEGQRRAEQAAARKDVPRLTEILSEERVTKRSAQRIVEALELCGQADVLS
                     KGRALAKGEKPLVQALDRLANVYRLLCETGFQEALLLDLGEFRGFDYYDGIVFDVFTD
                     GIGMELGGGGRYDHLIGRFGRNLPSTGFALSVDRLFKGLNLSERTKSVGPEVLVVAPL
                     GLSTRVVSVAQQLRQAGIRTVQQV"
     gene            complement(1179..2777)
                     /gene="serA"
                     /locus_tag="VN647_01310"
     CDS             complement(1179..2777)
                     /gene="serA"
                     /locus_tag="VN647_01310"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWG95692.1"
                     /translation="MAAAGMKILISDSLSKQGVEALEKAGFTVVVKSKMPKDELFKEI
                     KDADGLIVRSGTKVTAELIAAAEKLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNT
                     VTTAEHTMSMICAMSRRIPQATASVKAGKWEKDKFMGVELYNKVLGIIGAGQIGSHLT
                     KMAQGIGMSVVAFDPYLAPERAERMGVTMLDLEELFRQADVISVHTPLTPETRGIINA
                     QAIAKMKPGVLIVNCARGGIINERDLCEALKTKRVAAAAFDVFEEEPVKPDNPLLALD
                     NFICTPHIGAQTTEAQENVAIGIAEQIVDYFTKGVAKGAVNIPSVAPELLPRLQPFLT
                     LAEKLGTLQTQLVQGGIERVTVEYSGEVATLSIAPLTIAVLKGLLTPIMEHPVNYVNA
                     PIVAKERGIEVKEVKSTDAGDFTSLIRVRVETGKVSHQVAGTLYHKKEARVTEIEQFK
                     VEVVPEEHMLLIHNVDRPGVIGMVGKVLGDQGINIVRMQCALEKRGGDALLIIGSDTE
                     FPSAVLDQIRSSSNILSVKIANLS"
     gene            complement(2795..>3230)
                     /locus_tag="VN647_01315"
     CDS             complement(2795..>3230)
                     /locus_tag="VN647_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWG95693.1"
                     /translation="AFTPAVSLVIGLQEAFRMMRSEGLEKMFARQGRLAHAMREGVKA
                     AGLALFPKGSPSDALTAVCAPDGIDGQAIYKNLRVQYGMTAAGGQDHLKGKIFRLSHM
                     GYADTFDVIAALAATEMVLKGLGHPVKLGSGVGKAQEILLAK"
BASE COUNT          691 a         1003 c          818 g          718 t
ORIGIN      
        1 cgacctgttg aaccgttcgt atccccgctt ggcgaagttg ttgagcgacc gaaaccactc
       61 gtgttgacaa ccctagcgga gccaccacca acacttccgg tccaacggac ttcgtcctct
      121 cggaaagatt cagccctttg aagagccgat ctacactgag ggcaaatccg gtcgacggaa
      181 ggttccgacc gaaccgtccg attaagtgat catagcgtcc accgccgcct aactccatcc
      241 cgataccatc ggtaaagaca tcaaagacga tgccgtcata gtagtcgaac cctctgaatt
      301 ctcctagatc cagcaagagg gcctcttgaa atccggtctc gcagagcaat cgatagacgt
      361 tcgccagtcg atccaatgct tggaccaagg gcttctcacc cttcgccaat gcgcgccctt
      421 tcgagagaac gtcagcctgt ccgcaaagtt ccaacgcttc cacaattctc tgagccgacc
      481 ttttggtcac ccgctcttcc gataagatct ccgtaagcct cggtacatct tttcttgctg
      541 cagcttgctc agcccgcctt tgcccttccg gggaaagtcc cgcacgaacg agcagcccct
      601 tgaaaaaccc aacatgtccc aatgaaatct tgaaggagcg caacccgacc tgccgtaagc
      661 actcgatcaa caacatgatg atttcactat cggcggacgg atcatcggcc ccgatcagct
      721 cggcgcctac ttgaaagatt tctcgacctc gaccggcatg ttccggttca tgccgaaaga
      781 cagtcgcgcg gtacgacaag cgcaacggaa aatgcgcacc caccatgccc atcgctaccg
      841 tgcgggcgat ttgtgccgtg acgtcgggcc gcaacaatag aatacggccg gtcgtccgat
      901 ctacgatctt ataggagttc tctaacaacg tcggttcgag gccgggcgcc agcacatcga
      961 gatactcgaa cgtcggaagg atgatttcat cgtacccgaa acgattgaag tacgccaaca
     1021 atccggcttc cagatgccga acgtggcggg cggcttccgg caggatggtg gccatcccga
     1081 ccggcaccag tgagtgctcg cgcaaaagag ggaagggccc cgaagaagtc ctacgcatcg
     1141 gaggagcaga ggccataata acctgatcgc aagaatcgtt acgaaagatt ggcgatcttg
     1201 acggacagaa tattcgagct ggaacggatc tgatccagga ccgcagatgg gaattccgta
     1261 tcggaaccga tgatcagcaa tgcatcccct ccccgctttt ccaatgcaca ttgcatccgc
     1321 acaatgttga ttccctgatc tcccagaacc tttccgacca tcccgatgac cccggggcga
     1381 tcaacattat gaatcaacaa catatgctcc tccggtacaa cttcgacttt aaattgctcg
     1441 atctccgtga cacgggcttc tttcttgtga tacagcgtcc cggcgacttg atgtgacacc
     1501 ttgccggtct cgacccgaac ccgaatcagg cttgtgaaat ccccggcatc cgtgctcttc
     1561 acttctttga cttcgatgcc ccgctctttc gccacgatag gggcattcac gtagttcacc
     1621 gggtgttcca taataggagt gagcaagccc ttgaggacgg cgatggtcaa gggcgcgatg
     1681 gagagcgtgg cgacttcccc gctgtactcc accgtgactc gctcgattcc tccttgaacg
     1741 agttgagtct gaagcgtacc gagcttttcg gccaacgtca gaaagggttg caagcgaggc
     1801 aataattccg gagcgaccga cggaatattg acggcgccct tggccactcc cttggtgaag
     1861 tagtcgacga tctgttccgc aattccgatc gcgacgtttt cctgagcctc cgtcgtctgg
     1921 gctccgatat gcggggtgca gatgaaatta tccaaggcaa ggagcggatt gtcgggcttg
     1981 accggctctt cttcaaacac gtcgaatgcc gcagcggcga cgcgctttgt tttcaaggct
     2041 tcgcacaaat cacgttcatt aatgatgccg ccccgggcac aattcacgat gagcacgccg
     2101 ggtttcatct tggcaatggc ctgtgcgttg atgatcccgc gcgtctccgg cgtgagcggc
     2161 gtatggaccg aaataacgtc cgcctgccgg aagagttctt cgaggtccag catcgtcacg
     2221 cccattcgct cggcccgttc cggtgccaag tacgggtcaa acgcgacgac gctcatgccg
     2281 atcccttgcg ccatcttggt caggtgactg ccgatctgtc cggcgccgat gatgccgagc
     2341 accttgttgt agagctcgac acccatgaat ttgtcctttt cccatttgcc tgccttcacc
     2401 gaggcggtgg cttggggaat gcgccgactc atcgcacaga tcatcgacat cgtgtgctcg
     2461 gcagtggtga cggtattgcc ccccggcgta ttcatgacga cgatgccgcg acgggtcgca
     2521 gcaggggtat cgacattgtc caggcccgat ccggcccttc cgacgacctt gagtttctcc
     2581 gctgcagcga tcagttctgc cgtcaccttg gtgccggatc gcacaataag tccgtcggca
     2641 tctttgatct ctttgaacaa ctcgtccttc ggcattttag atttcaccac cacggtgaat
     2701 cccgccttct cgagcgcctc gacaccttgt ttcgacaagc tatcgctgat caaaattttc
     2761 attcccgctg cggccatgtg cgtcctcgcc gtggttactt tgccagtaag atttcttgcg
     2821 cttttccgac accgcttccc aacttcaccg gatggccaag ccctttcaga accatctcgg
     2881 tcgcggctaa tgctgcgatc acgtcgaacg tatccgcata tcccatatga gacagtcgga
     2941 atatcttccc cttgaggtga tcctgtccac cggcagccgt catgccgtat tgcacacgca
     3001 ggttcttata gatcgcctgc ccgtcaattc catcaggggc gcacaccgcc gtcaaggcat
     3061 cgctgggcga tccctttgga aatagtgcca gcccggcagc cttcacacct tcgcgcatgg
     3121 catgggccaa ccgtccctgc cgtgcaaaca ttttttccag cccttccgac ctcatcattc
     3181 ggaacgcctc ttgaaggccg atcacaagcg agacggccgg ggtgaaggca
//
LOCUS       DATNDD010000061         5358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1832448, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000061 DATNDD010000000
VERSION     DATNDD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832448"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VN647_01320"
     CDS             complement(<1..367)
                     /locus_tag="VN647_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG95694.1"
                     /translation="MYSARILLADDHPLVLESVKQLLEPDFSVVGTAQTGQQVLEEAQ
                     KLNPDIVMLDANMPGMSGFEAARRLKTLLPTIKIIFVTMLTEAIAVSEGFRAGAMGYV
                     LKQDASDELHTAVKSVMAKQ"
     gene            complement(611..1915)
                     /locus_tag="VN647_01325"
     CDS             complement(611..1915)
                     /locus_tag="VN647_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95695.1"
                     /translation="MQNSFWLKMLSAASLFLLCVTSWVGAEESAGAVNEARLVAQYNY
                     SIHILAMLVVGFGFLMVFVRRYGFGATTGTYLVVATGLPLYMLLRANGMVGHPIQAHS
                     VETLMLAEFSVATALIAMGAVLGRLRVFQYALLTLLLVPLYALNEYLVLDNGSGLTKG
                     FQDSAGSIVIHAFGAYFGLAASLVLTTAQQRTQPIESDPTSDRFAMLGSMVLWLFWPS
                     FATAIVPFEQMPQTIVNTILALSGATLATYFLSTHFHHGKTSMVDMANAALAGGVSIG
                     STCNLVSPTGAFGIGLLAGALSVIGFIFIQPMLESKIKLVDTCGVHNLHGMPGLLGGL
                     CAIAVVPSVVGVQLVGMAMTLVIAIVGGVIAGMVIRATGTTEQAYEDCHEFSHVPGPE
                     SEHKVEELALGAKADIEALQKELFARTGAHIRPAREEPRPIA"
     gene            2579..3379
                     /gene="thiD"
                     /locus_tag="VN647_01330"
     CDS             2579..3379
                     /gene="thiD"
                     /locus_tag="VN647_01330"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWG95696.1"
                     /translation="MSKTLKQVLTIAGSDSGGGAGIQADIKAMSANGVFAMSVITAIT
                     AQNTEEVTDVLELPPAIIASQLDAIFDDFDVAAVKTGMLSSAVIVEVVVKLMKPQNVV
                     NLVVDPVMISKSGHSLLRPDAIEAIRMKLLPLSLVVTPNVHEAQQLSGIEIASLADAR
                     RAAKVIHGFGCKHVLIKGGHLINERATDLLYDGRFFNVLKGEFIETRHTHGTGCTFAS
                     ALAAHLARGRSVLDSAQAAKSYVTEAIRHGLAIGHGQGPTDHFYFLER"
     gene            3389..4714
                     /locus_tag="VN647_01335"
     CDS             3389..4714
                     /locus_tag="VN647_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95697.1"
                     /translation="MFARTIPPVMFCITGFSWLVLASILGMATLIGLVHGTPLPSWVR
                     ALHVHATLLGGVTQVILGGFILMIPPPNSANRKEADSHPLTFWAMNSGLIGMLVGFWL
                     HQDLVVSVSGFMVIAAFLSVIYTVWIRASRTWKFSFKESWYYALSLFCLVAGSAFGEI
                     MAFGLIPESHGYVRLAHIHLVVIGFVVLAIIGMVHHLLPTVWNRPRSVPKLARIGTIL
                     IPIGVAVLIGGFLNSSVPIELSAAAILFIGGILWIGSLFRTWLSSTHTGSAASDHLFI
                     SVFFLIFTVILGILVGANNLSSPSVLPYGRLHLAAYTHMAFIGFVMNAVIGAFSYLLP
                     IALAADRISSHKKRGTYLDRLTTIMNRWRTVQIATLNLGTMGLGILAALTWNVPLTSM
                     SIRVASWTSLSLLMTGLVLCSVKLTSIIAKKPDQLQTGQISTDELKLTA"
     gene            4774..>5358
                     /locus_tag="VN647_01340"
     CDS             4774..>5358
                     /locus_tag="VN647_01340"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG95698.1"
                     /translation="MEERFSFLDPHGHQVAAVLTVPNGETDKISILCHGFLSSKTSST
                     NNALTRMLVGQGIAAFRFDFFGQGESEGPFDQITVSLAVEQTQRAVELMRDRGYRHIG
                     LMGSSFGGLVSILTASQRTDLACLALKCPVIDFAEELRLEFGDDGMALWKATGTIPNI
                     MGGPDRITLHYAFYEDALRQIAYVPARSITAPTVI"
BASE COUNT         1218 a         1575 c         1356 g         1209 t
ORIGIN      
        1 tttgcttggc catgacactc ttgaccgctg tatggagttc gtcggaagcg tcttgcttca
       61 agacataacc catcgctccc gcacggaacc cctcgctgac tgcaatagct tcggtcagca
      121 tcgtgacaaa gataatttta atcgtaggca agagggtttt cagtcttcga gccgcttcaa
      181 atccgctcat ccccggcatg ttggcgtcaa gcattacgat atccgggttc aatttctggg
      241 cttcttccag cacttgctgc cccgtctgcg cggtccctac tactgaaaag tccggttcga
      301 gcaattgctt gacgctctct aaaactaacg ggtggtcgtc agcgagcaaa attctcgcac
      361 tatacataac ggatcctctt cgtcatacga ccaacccgtt tctgctcaac atctcgttcc
      421 atacgatgat tcacccaacc caacgaaaac aaggaccaac gctgtaagca catgatggca
      481 gtcactacac gataatccga ctagggatac ccctagtgtg gccataaagc tgtgaccgtt
      541 catatggccg cgacagtata cagcccgata taatgctcac cgctcagtcg tccctgattg
      601 agcgactgac ttacgcgatc ggtctcggct cctcgcgcgc gggacgaata tgcgcccctg
      661 tccgggcaaa cagttccttt tgcaaggctt cgatatcggc ctttgcgcct aacgcaagct
      721 cttcaacctt gtgttcactc tccggccccg gcacatggga aaactcatgg cagtcttcat
      781 acgcctgttc tgtcgtgcct gtggctctga tcaccatgcc ggcgatgacc cctccgacaa
      841 tggcgatgac gagcgtcatc gccatgccca cgagttggac cccgacaaca ctcggaacga
      901 cggcgatcgc gcaaagtcct cccaacaggc cgggcatccc atgcagattg tggacgccgc
      961 aggtatcgac caatttgatt ttcgactcca gcatgggctg aataaaaatg aaccctatca
     1021 ccgaaagagc accggcgagc aatccgatac caaacgcacc ggtgggactc acaaggttac
     1081 aggtcgagcc gatggagacc ccgcccgcca acgctgcatt cgccatatct accatcgagg
     1141 ttttcccatg atgaaaatgg gtgctgagga aataggtggc aagcgtggcc ccgcttagag
     1201 caaggatcgt attcacgatg gtttgaggca tttgttcaaa gggcacgatt gcggttgcaa
     1261 aactcggcca aaacagccac aaaaccatgg aaccgagcat tgcgaaccga tcagacgtcg
     1321 gatcggattc gatcggttga gtacgctgct gcgcagtggt caaaaccaga gacgcggcca
     1381 atccgaaata ggcaccgaac gcgtggatga cgatcgaccc cgccgaatcc tgaaatcctt
     1441 tggtaagccc cgagccattg tccaacacca aatattcgtt caacgcatag agcggcacca
     1501 acaacaacgt gagtagcgcg tattgaaaca cgcgcaatcg cccgagcacc gcccccatcg
     1561 cgatcaacgc cgtcgccacc gaaaactcag ccagcatcag cgtctcgacc gaatgggcct
     1621 gtatcggatg ccccaccatc ccgttggcgc gcaacaacat gtacaaaggt agtccggttg
     1681 cgacgacgag ataggtaccg gtcgttgccc cgaagccata ccgccggacg aacaccataa
     1741 gaaatccgaa gccgaccacc aacatggcca ggatgtgaat cgagtaatta tattgtgcga
     1801 ccaggcgcgc ctcattgacg gcacccgccg actcttcggc accgacccaa ctcgtgacgc
     1861 acaagagaaa cagacttgct gcgctgagca tcttcaacca gaagctgttc tgcattgagt
     1921 cctccttgga caataggagt gatggggggc tataggggtg gcagtgtact ccactagcaa
     1981 gcgcccttgt caaaccccac ctatggctta ctcgcggaag cggtaagcct caataagtca
     2041 gcagttaacg agagaactat gacgaagcag atgataaata aggaaaaatt catgttctca
     2101 acccgaaacc tgtggtaggg ctccaaatat tccacggttt ccatctcgac catcggcttc
     2161 gtatccatga tgatctggag aaacttcagc cactccaggc cgaagaaaac tacccatgtt
     2221 ccagccgtcc aattcccggg aaaaagaccg catccaagca gtccacgtca aagctcaacc
     2281 agacggcgac catcctattc catgcccctt caacacctgt ttcgcggcac cattttttta
     2341 cggcccgcta gacagcagac cttccctgag tataatgaca tcagttcatg cgtatgatcg
     2401 gactcctggt actgctcgtc ggtttcgcaa catctttctc acatccgaca tcatgtgccg
     2461 gggcgagtta tgaggagagc ctcagcagtt ggcggacggg ctcctacaga ggagactgat
     2521 gggttgaaga tcggctcgcc gtcggaacct gcgcagacca ctccgtaaag gatcattcat
     2581 gtcgaagacg ttgaaacagg tgctcacgat cgcgggctcg gattccggtg gaggggcagg
     2641 tatccaggcg gacatcaaag ctatgtccgc caacggtgtg ttcgccatgt ccgtcatcac
     2701 ggccatcacg gcacagaata ccgaagaagt gacggatgtc cttgaattac cgccggccat
     2761 tattgcatcg caactcgatg caatcttcga cgacttcgac gttgccgccg tcaagaccgg
     2821 aatgttgtcc tcggcggtca tcgtcgaggt ggttgtgaag ttgatgaaac ctcaaaacgt
     2881 cgtgaatctg gttgtggacc ccgtgatgat ctccaagagc ggtcattccc tcttgcggcc
     2941 ggacgccatc gaggccatta ggatgaagtt gctccccctc tccttggtcg tgacaccgaa
     3001 cgtacatgag gcacagcagc tgtcggggat tgaaatagcc tcgttggccg atgcccggcg
     3061 agcggccaaa gtgattcatg gcttcggatg caagcatgtt ctgatcaaag gcgggcacct
     3121 gatcaacgaa cgagcgaccg atctgctcta tgatggacgt tttttcaatg tgttgaaggg
     3181 agagttcatc gagacccgcc atactcatgg caccggctgt acgttcgcct ccgcattggc
     3241 ggctcacctt gcacgaggga ggtccgtctt agattctgcg caagccgcca agtcctatgt
     3301 tacggaagcg atccgacatg gcttagcgat cgggcacggg caaggcccga cggaccattt
     3361 ttatttctta gagcgatagc acatgcctat gttcgcacga accattcccc ccgtcatgtt
     3421 ttgcatcacg ggtttcagct ggctggtgct ggcgtcgatt ctgggcatgg caaccctaat
     3481 cgggctggtt catggcacac ccttgccgtc gtgggtcaga gcgctccatg tccatgcaac
     3541 cttgctcggt ggtgtaaccc aagtcattct cggcggattc atcttaatga tccctccgcc
     3601 gaattcggca aaccgaaaag aagcggactc ccatcccctc acattttggg cgatgaacag
     3661 cggcttgatt ggcatgctcg tggggttctg gttgcaccag gacctagtcg taagtgtgtc
     3721 cggcttcatg gtgattgccg cgtttctttc cgtcatctac accgtttgga tccgtgcgag
     3781 caggacttgg aagttctcat tcaaagaatc ttggtactat gcgctttccc tcttctgcct
     3841 tgtcgccggg tcggccttcg gagaaatcat ggcattcggg ctcatcccgg aatcccatgg
     3901 ctatgtgcgg ctcgcacaca ttcatctggt ggtgatcggc tttgtggtct tggcgatcat
     3961 cgggatggtg catcatcttc tgccgacggt ctggaaccgt ccgcgatcgg tccctaagct
     4021 cgcccgtata ggaacaatct tgataccgat aggcgtggcc gtcttaatcg gtggattctt
     4081 gaactcatcg gtcccgattg aactgtccgc cgccgccatt cttttcatcg gcgggatcct
     4141 ctggatcggc agcctgtttc gaacgtggct ctcctcaacc cataccggca gcgccgcatc
     4201 ggatcatctc ttcatcagcg ttttctttct catctttacc gtcattctag gcatacttgt
     4261 gggagcaaat aatctctcca gtccatcggt attaccctat ggcagactcc accttgcggc
     4321 ttacacgcac atggcattca tcggcttcgt catgaatgcc gtcataggcg cattctccta
     4381 cctacttccg atcgccctcg cagccgaccg catttctagt cataagaaac gggggaccta
     4441 tcttgaccgg ctcacgacca tcatgaaccg atggaggacc gttcaaatcg ccaccctcaa
     4501 cctaggaacc atgggactcg gcatcttggc ggcactgacc tggaatgtgc cgctgacctc
     4561 aatgtccatc cgtgttgcaa gctggaccag ccttagcctc ctcatgaccg gcttggttct
     4621 ctgctcggtc aagctgacct ccattatcgc caagaaaccg gaccagcttc agacaggaca
     4681 gatctccacg gacgaactca agctcacggc ctgacttccg acgggttcga cttccatcct
     4741 gacctcagac accgactctg atacaatcct tccgtggaag aacggttttc atttctcgat
     4801 cctcacggcc atcaggtagc cgccgttttg accgtcccca acggagaaac ggacaaaatt
     4861 tccatcctgt gtcacgggtt tctttcatcg aaaaccagct cgacgaacaa tgcactgacc
     4921 cgcatgctgg tcggtcaagg catcgccgct tttcgcttcg atttcttcgg tcagggagaa
     4981 agtgagggcc cgttcgatca gatcacggtc agcttggcag ttgaacaaac acagagagcg
     5041 gtcgagctca tgagagatcg cgggtatcgg cacatcggac tgatgggatc aagtttcggc
     5101 ggtctggtct cgattttgac tgcttcgcaa cggacagact tggcctgcct cgccttgaaa
     5161 tgtccggtca tcgacttcgc cgaggagcta cggctcgaat tcggggatga tggaatggca
     5221 ctatggaagg caacgggcac gattccaaat attatgggcg gccccgaccg gatcacgctt
     5281 cactatgcct tttacgaaga tgctcttcgg cagatagcct atgtccccgc acgatcgatc
     5341 acggctccga ccgtcatt
//
LOCUS       DATNDD010000062         6935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4041309, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000062 DATNDD010000000
VERSION     DATNDD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4041309"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1127)
                     /locus_tag="VN647_01345"
     CDS             complement(267..1127)
                     /locus_tag="VN647_01345"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWG95699.1"
                     /translation="MSYGPPVISIDVEDWPQSTWDRSLPITERAAENTRHVLQLLRET
                     EVRATMFVLGKFAERFPQVVKEIQADGHEMACHGYGHLEIGRQSREEFLADVRRSKDL
                     LEQIIGMPVRGYRAPDFSIVQDTLWALDLLAEAGFEYDSSIVPARLPRYGIAGWPVLP
                     TRVRLDGGGSILEAPLATFQTLGRNLPVGGGGYHRLLPGLASRYFARRIMTDAPFVFY
                     CHPYEFDVYEFKEIPVRVPLTVQFYQGAGRRWFEQRFRAFVRCFGGQPMCAMLSSQPW
                     QEFQPCSLNH"
     gene            complement(1124..1780)
                     /locus_tag="VN647_01350"
     CDS             complement(1124..1780)
                     /locus_tag="VN647_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWG95700.1"
                     /translation="MSQEPNAATFFNSFAEAFDTIYDQKRNPFMRWVDGKFRSDMFLR
                     YALTFEALEPLTGRTVLDVGCGSGPYIAEAFKRGAERVTGLDPAPNMLALVRQRLAQT
                     GVAERRYELVTGLFPETPVGPHDYAIVMGVMDYVEDAAAFLKALRPAVQRLAVVSFPS
                     THWFRTPFRKFRYQLRHCPVYFYTDTSIRKLCERAGFSDIRIQKIPGAGMDFHVSLRP
                     "
     gene            complement(1777..2799)
                     /locus_tag="VN647_01355"
     CDS             complement(1777..2799)
                     /locus_tag="VN647_01355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWG95701.1"
                     /translation="MLSVVMLCRNERHHIEACVRSILDQERPPEGIEVIVVDGISDDG
                     TRQILARLAKEHPELRVVDNPRRITPCAMNVGIREARGRYIAILGAHCQYAPDYLSTC
                     AALLQEHPEVCCVGGPAVSIGKGLFGQAVAAAMSHPVGIGNAKHRYPTYEGYAEGACY
                     PVFRKEVFDKIGFYDEMLVRNQDDELNYRLAKHGEKIFISPRARYSYFIRETPSRLFQ
                     QYFEYGYWRVAVLRKHRIPASFRQIVPPLFMAVMLASVIVGLSLPGWWRLIAAAIPVL
                     YGVTLLAVGVVQSHKTGWRVAILFPVAVAVMHVAYAMGFVLGILKKGTDHIVRNSGAE
                     QQRSMS"
     gene            complement(2833..3237)
                     /locus_tag="VN647_01360"
     CDS             complement(2833..3237)
                     /locus_tag="VN647_01360"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HWG95702.1"
                     /translation="MDRTSQLPTTKDLRQFGFLVGGVFAVIGLWPVVFRSESPRLWAM
                     ILGGLLIVLGALAPQSLKQIHGGWMKIGHILGAINTKIILGIIYYFLITPMGLIMRFM
                     GKDPMHRALAQGTDTYRIVRAPRSRHHMRNQF"
     gene            complement(3249..5093)
                     /locus_tag="VN647_01365"
     CDS             complement(3249..5093)
                     /locus_tag="VN647_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HWG95703.1"
                     /translation="MSHILGISAFYHDSAACLVRNGDIIAAAQEERFTRKKHDPGFPS
                     QAVTYCLKEGGITLGDLRYIVFYDKPLVKFERLLETYLAFAPKGLQSFVAAMPVWLKE
                     KLLLRNLLAKEFVALAPEMNQSTLPPLLFGEHHESHAASAFYPSPYDKAVVLCMDGVG
                     EWATTSAWLGEGNALTPLWEIPFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEP
                     KYVKAIYDHLLDLKPDGTFRLNMDYFNYCTGLTMTGDKFDDLFGGPPRKQESKLTQRE
                     MDLARSVQEVTEEVMLRVTRTLHRETGIEYLCMAGGVALNCVGNGRILREGPFKGIWI
                     QPAAGDAGGAIGAALSAWYRYDDQPRVSTGVDRMRGSYLGPRHTNAEIEEFLKRLEAP
                     YELLDDDHLYDRVAKDLAEGKVVGWLQGRMEFGPRALGGRSILGDARNTKMQSVMNLK
                     IKYRESFRPFAPSVLRERVSDFFEMNTDSPYMLLVAPVMEKRRLPLPANRSDLWGIDL
                     LNIPRSDVPAVTHLDYSARIQTVHAETNPRYYRLLKSFETQTGYPVLVNTSFNVRGEP
                     IVSTPEDAYRCFMRTEMDVLVLENCVLYKTQQKPLENDTNWQKEFELD"
     gene            complement(5684..5836)
                     /locus_tag="VN647_01370"
     CDS             complement(5684..5836)
                     /locus_tag="VN647_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HWG95704.1"
                     /translation="MSGFVMELWAFMKERKKFWLLPILVVLLLFGTLIVLTQGSAVAP
                     FIYTLF"
     gene            complement(6193..>6935)
                     /locus_tag="VN647_01375"
     CDS             complement(6193..>6935)
                     /locus_tag="VN647_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWG95705.1"
                     /translation="VTFGWGVDQDKTFAARLEPLLQGRLHRPVRAINSGVGGYNTVQE
                     VTYFKQEGIALQPDLVMLTYVKNDIEVNKGPFDPWAQGSLWGKSFPDMVMTMVGKLWL
                     YRLIYHTYHYALPESQGGPATTSLHEGAGWRQSMAALHELVVMCEERHIPLIVFFHRL
                     DSSESKPLFDAVVQHAQGVPIKDMGQWYGGLRVSSLVNSKVDGHPNAEGHRVMAEHMA
                     EDIAGYLAQLTGRASLGSPDIDVAIAGP"
BASE COUNT         1814 a         2005 c         1600 g         1516 t
ORIGIN      
        1 cactaggttt tttccccaat atccggcacc gattaccgca atcttcagga ctgtctcttg
       61 accatctcct gatataccag ccatgacatt ctcctcatga tttacttcaa tatacccaaa
      121 ccctcatcgg taagactaag atacggcact tcaagacaca atggatgagt caaattgttc
      181 ggcaacttag gccctctctg caaacttatc gcagtaaagc gtcaggagct cggactgggt
      241 acatatctct tcgagaggta attggctcaa tgattcaagc tgcagggctg aaactcctgc
      301 catggttggg aggaaagcat agcacacata ggttgtccgc caaagcatct gacgaaagcc
      361 cggaagcgct gttcaaacca tcttcgccct gcaccttggt aaaattgaac cgtgagcggg
      421 acacggaccg gaatctcctt gaattcgtag acgtcaaatt cataaggatg gcagtaaaac
      481 acgaacgggg catcggtcat aatccgccga gcaaagtacc gactcgcaag gccaggcaac
      541 aaacgatgat acccgcctcc tcctacaggc aaatttctcc cgagggtttg aaaggtggcc
      601 agcggagctt ccagaatact gccgcccccg tccaggcgaa cccgagtcgg tagaacgggc
      661 cagcccgcta tgccatagcg cggaagacgg gccggcacga tgctggagtc atactcaaaa
      721 ccggcttctg caaggaggtc gagagcccaa agcgtatctt ggacaatcga gaaatcaggc
      781 gcacgatatc cccttaccgg cattccgatg atctgttcga gaagatcctt ggagcgacgg
      841 acatcggcga gaaattcctc gcgtgactgt cggccaattt ccagatgccc gtacccatgg
      901 catgccattt catgcccatc agcttggatc tccttgacga cctgaggaaa gcgctcggca
      961 aacttaccga gcacgaacat ggtggcccgc acctcggtct cccgtagaag ctgtaagacg
     1021 tgccgggtat tttcagcagc tcgctccgtg atggggagac tccgatccca cgtggattga
     1081 ggccagtctt ccacgtcaat cgaaatgaca ggcggaccat aactcatggc cgtaaactta
     1141 cgtgaaagtc catgcccgct cccgggatct tctggatccg gatatctgaa aaacctgccc
     1201 gttcacagag tttacgaata ctggtgtccg tataaaagta gacaggacag tgccgcaatt
     1261 gatagcggaa ttttcggaag ggggtcctga accaatgtgt actggggaat gacacaacgg
     1321 caagacgttg gactgcaggc cgcagggctt tcagaaaagc cgccgcatcc tcgacgtaat
     1381 ccatcactcc catcacgatc gcatagtcgt gaggacccac cggtgtttcc ggaaacaacc
     1441 ctgtcactaa ttcgtaccgc ctctcagcca cccccgtctg ggccagccgt tggcgcacca
     1501 aggccaacat gttaggcgcg ggatcaagtc ccgtcactcg ctccgcaccg cgcttgaagg
     1561 cctccgcaat gtacggccct gagccacaac ccacatccag cactgtacgt cccgtcagcg
     1621 gttccagcgc ttcgaaggtc agggcatagc gcaggaacat gtcactccga aacttaccgt
     1681 ccacccatcg cataaaagga ttgcgcttct gatcgtagat ggtgtcaaag gcttcggcaa
     1741 aactattgaa aaaggttgcc gcgttaggtt cctgactcat gacatgctcc tttgttgctc
     1801 cgcaccacta ttccgcacta tatgatcagt acccttcttc agtatcccga gtacaaatcc
     1861 cattgcatag gccacatgca tgacggccac tgcgacggga aacagaattg ccactcgcca
     1921 gccagtctta tgactctgca ccactccaac agccagtaat gtcaccccat aaagcaccgg
     1981 tatcgcagca gcgatgagtc gccaccagcc aggtaacgat agtcccacta tgacactcgc
     2041 caacataacg gccataaata ggggcggcac aatctgtcga aaggaagccg gaatccgatg
     2101 ttttctcaat accgccaccc gccaatagcc gtactcgaaa tactgctgga aaagtctgga
     2161 gggtgtctct cggatgaaat aagaatatcg ggcacgcggt gaaataaaga tcttttctcc
     2221 atgtttggca agccggtagt tgagttcgtc atcttgattg cgaaccaaca tttcatcata
     2281 gaatccgatc ttatcaaaca cttcttttcg aaacaccgga tagcaagctc cttccgcata
     2341 gccttcataa gtcggatatc ggtgtttagc attgccgatt cccaccgggt gggacatcgc
     2401 cgccgcaact gcttgaccga aaagaccctt gcctatgctc acagccggtc ccccaacaca
     2461 acacacctca ggatgctcct gaagaagagc cgcgcatgtc gacaggtaat ccggcgcata
     2521 ctgacaatgg gcaccaagaa tggcgatata ccgaccacgg gcctcacgaa taccgacgtt
     2581 catcgcgcag ggtgtaatac gtcttgggtt gtcgacaacc cgcagttctg gatgttcttt
     2641 ggcgagtctg gctaaaatct gtcgcgtgcc atcatctgaa attccatcaa cgacgataac
     2701 ctcaataccc tcaggaggac gttcctggtc cagaatcgat cgtacacagg cctcaatatg
     2761 gtggcgctcg tttcgacaaa gcatgactac agaaagcata ggaatccttt gccgacaaga
     2821 accacgctcg acctaaaatt gatttcgcat atgatggcgt gatcgcggag cccgtacgat
     2881 gcgataggtg tcagtaccct gcgccaaggc acgatgcatc ggatccttac ccatgaagcg
     2941 catgatcaga cccattgggg tgataagaaa ataatatatg atgccgagta tgattttcgt
     3001 attaatcgcg ccgagaatgt gaccgatctt catccatcca ccgtgaatct gcttcaagct
     3061 ctgaggggca agcgcgccca gaacaatgag caggccccca agaatcatcg cccacaagcg
     3121 gggcgactca cttcggaaca caacaggcca caatccgatt actgcaaaca cacctccaac
     3181 aaggaacccg aactgccgca aatccttcgt ggtaggcagt tgacttgttc gatccatcgc
     3241 gcaatctcct aatcaagctc gaattctttt tgccagtttg tatcgttttc cagcggcttt
     3301 tgttgcgttt tatacaggac gcagttttcc agcacgagca cgtccatttc agtccgcata
     3361 aaacatcggt aggcatcctc cggtgtggat acaatcggct ccccgcgaac gttgaaagat
     3421 gtattgacta gaacgggata gcctgtttgc gtctcgaacg acttaagcaa ccggtaatac
     3481 ctcgggttgg tctccgcatg aacggtttgt attcgggccg aataatccaa atgcgtgacc
     3541 gctggaacat cggaccgcgg aatattcaac agatcgattc cccataaatc ggatcgattg
     3601 gccggcaagg gcagccgtcg cttctccatc accggcgcca ccagcagcat gtagggactg
     3661 tccgtattca tctcaaaaaa atccgacact cgctcccgca ggaccgatgg cgcaaaggga
     3721 cgaaacgact cgcgatactt gatcttcaga ttcatcaccg actgcatctt cgtatttctg
     3781 gcgtcgccaa ggatacttcg accgccaagt gcacgtggac cgaactccat ccggccctgc
     3841 aaccaaccga caaccttccc ttcggcaaga tcttttgcca cccgatcata gaggtgatcg
     3901 tcgtccagca attcatacgg cgcctctaac cgcttgagga actcctcgat ttcggcattg
     3961 gtatgtcgtg gtcccaggta actgcctcgc attcgatcaa cgccggtcga taccctgggt
     4021 tggtcatcat aacgatacca agcactcaac gcagcaccga tcgcccctcc cgcatctcca
     4081 gctgccggct gaatccagat ccccttgaac ggaccttctc gtagaattcg tccattcccc
     4141 acacaattga gggcgacacc gccagccatg cagagatact caattccggt ttcacggtgt
     4201 agagttctgg tgacacggag catgacttct tcggttactt cttggactga acgggccaaa
     4261 tccatctcac gttgcgtcaa cttactctct tgcttccgcg gaggcccgcc gaacaagtca
     4321 tcgaacttgt ccccggtcat ggtcaaccca gtgcagtagt tgaagtaatc catgttcaag
     4381 cggaatgtcc cgtccggttt gagatcaagg agatggtcat agatggcctt gacatacttc
     4441 ggctctccat acggggctaa acccattact ttatactctc cggaattgac cttgaagccg
     4501 gtatagtagg tgaaggcgga ataaaggagc cccagagaat gagggaacgg aatttcccaa
     4561 agcggcgtca atgcattccc ctcgccaagc caggcagacg tcgtggccca ttcccctaca
     4621 ccatccatac acagaacgac cgccttgtca tagggcgacg gataaaatgc ggaggcggca
     4681 tgcgactcat ggtgctcacc gaacaacagc gggggaagtg tcgactggtt catctccggc
     4741 gccaacgcga caaactcctt cgcaagcaga tttcggagta agagcttttc cttgagccat
     4801 accggcatag ccgccacaaa cgactggagc cccttcggag cgaaggccag gtaggtctca
     4861 agtaaccgct caaatttcac gagcggttta tcataaaaaa cgatgtatcg gagatcaccc
     4921 agagtaattc ctccttcctt caaacagtaa gtcactgcct gagaaggaaa acccgggtca
     4981 tgtttctttc gggtaaaccg ttcttcctga gcagccgcta tgatatctcc attacggacg
     5041 aggcatgccg cactgtcgtg gtagaaggct gagattccca gtatatggct cataactcaa
     5101 ctccgccaaa ggttcgtaaa ccgctttcgg atgatgcgaa tcacggaatc ggattcgctt
     5161 gggcaggtga tcatttacct aggcaaccaa agagattcgc aaacataaaa gcgtccccca
     5221 aagccgtttg gcacaaagca catcagtaaa taacatcaga gaatcgaaac cttaagacat
     5281 cgtggtcagc aagcacgatt ctgtccatcc cgccaggaac aaccgagtca ccttgccggg
     5341 taggaagttc catcgatgca cctgaacggt cggcaccatc aacttcccta tgaaacagca
     5401 tagataatct cgactaaaga aacatcgtat caggaagtac gcgttcaaaa cacccacacc
     5461 ggcagccgca ccgctaagtg ctatcagtag ggacatccta aattgtagcg gtcgtcatgt
     5521 ctataccgca ctcgagggga ataaggaatt tctcgtaact gctataccaa cggcaattga
     5581 gttcgtcatc ataaaacgcc ctacatctca agcatcctcc tattcaccac caaaggggaa
     5641 ccgcaatgat accactccca gcttgaagag aaagcttcgg tcattagaac agagtataga
     5701 taaatggagc gaccgcggag ccttgcgtca acacgatcag cgtcccaaac aaaagcagaa
     5761 ccaccagaat cggcaatagc caaaatttct tgcgctcctt cataaaggcc cacagttcca
     5821 tgacaaagcc actcatgaca tccctccttt gctccaaacg tagttgaaag tagcttcata
     5881 caacgtatgc acaattaaat acgacataaa tgcgacaacg attctgccat cgacgttcgc
     5941 ttgcgaacag gactatacct cagcgcctga tgatctctcg attctgaagc caattgctct
     6001 ttgattgatg aaacaattct ttgaacaact tttccatccc acgatgggat gggttttcct
     6061 ttttatatcg accgccaaga atgcctcgta tagcctacct aataaaagtc tcaagaagca
     6121 tctcccgtca acaccactat tgaagctgtc ctcaaatcag gctttcagag gagaaccacc
     6181 tcgttgatca attcatggac cagcaatagc gacatcaatg tctggcgaac ccagacttgc
     6241 gcgacctgtt aactgggcaa ggtaacccgc aatatcttcg gccatgtgct ccgccattac
     6301 gcgatgaccc tccgcattgg gatggccgtc tactttggag ttcactaagg aagatacacg
     6361 gagccctcca taccattgtc ccatatcttt tatcggcacc ccctgggcat gctgaacgac
     6421 cgcatcaaac aatggcttgc tctcgctaga atccaaccga tggaagaata caatgagtgg
     6481 aatgtgtcgt tcttcacaca tcactaccag ctcgtgtaac gccgccatgg actgacgcca
     6541 ccccgctcct tcatgcaaag aagtcgttgc tggtcctcct tgagactcag gcaaagcata
     6601 gtgataggta tgatagatca atcgatagag ccagagcttt cccaccattg tcatcaccat
     6661 atccgggaaa gactttcccc acagagaacc ttgagcccat ggatcaaaag gccccttgtt
     6721 cacttcgata tcattcttca cataggtcaa catcactaaa tccggctgca gggcgatccc
     6781 ttcctgcttg aaataggtca cctcctgcac ggtgttgtac cccccgaccc cgctattgat
     6841 cgcccgcacc ggtcgatgca gccgtccctg cagtaacggc tcgagccggg cggcgaacgt
     6901 cttgtcctgg tccacacccc atccaaaggt gaccg
//
LOCUS       DATNDD010000063         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_79483, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000063 DATNDD010000000
VERSION     DATNDD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79483"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /gene="gcvP"
                     /locus_tag="VN647_01380"
     CDS             complement(<1..2157)
                     /gene="gcvP"
                     /locus_tag="VN647_01380"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HWG95706.1"
                     /translation="MVRCPIDGDTSHRPREPNGGTAGSMITQNWLQPTDDFVHRHLGP
                     TRADIQEMLASLGLLSLDTLADTAIPPDLRFRRSLDIPDGDGEQAVLARLKGIASQNK
                     VYRSLIGMGYYNCVTPGVIQRNILENPAWYTQYTPYQAEISQGRLEALVNFQTMVADL
                     TGLPLANASLLDEATAAAEAMAMCYTIARNAGEERKEFFVSRDCHPQTLAVLQTRAEP
                     LGIVIKTGVASSVDCSSPQLCGILLQYPATDGYVGDFSTLVAQAHEADVRVAVATDLL
                     ALTLLRSPGEFGADIAVGSTQRFGVPIGFGGPHAAFLATREEYKRQVPGRLVGISKDV
                     TGQSAIRLSLQTREQHIRREKATSNICTAQVLLAVMAAMYAVYHGPDGLRRIAERVRG
                     LTLLLAEGLRRLGFDVLPKVFFDTIRVSVPKAQADHIATRANEQGINFRSYEDGSIGV
                     SLDEVSSEEEVQRLLRIFVGHDHLPFRLSDLAADIDFSYSSPLARTSKYLTHEVFHRY
                     HSEHEMLRYLHRLQTKDLSLVHSMIPLGSCTMKLNATAEMLPVTWPEFARLHPFAPGE
                     QTLGYRTLFGQLESWLAEIAGFAAFSLQPNAGSQGEYSGLMVIRAYHRSKGETHRDVC
                     LIPVSAHGTNPASAAMVGMEVVVVACDRNGNVDIDDLEAKAAQYRDRLSALMLTYPST
                     HGVFEAGVRRICQIVHTHGGQVYIDGANMNAMVGLCR"
BASE COUNT          519 a          668 c          686 g          489 t
ORIGIN      
        1 acggcagagg cccaccatgg cattcatatt ggccccgtcg atgtagactt gaccgccatg
       61 ggtatggacg atttggcaaa tgcgccgcac gcccgcctca aatactccgt gggtcgacgg
      121 atacgtgagc atcagggccg acaatcgatc tcgatactga gctgccttgg cttccagatc
      181 gtcgatatcc acgtttccgt tccgatcaca cgcgacgacg acaacctcca tgccgaccat
      241 ggcggcgctg gcggggttcg tgccgtgggc tgagacgggg attaagcaga catctcggtg
      301 tgtttccccc ttcgaccgat ggtaggctcg aatcaccatc aggccggaat attcgccctg
      361 ggaccccgca ttcggttgga gggaaaaggc ggcaaatccg gcaatctcag ccagccacga
      421 ttcgagctgt ccgaacaagg tccgatagcc gagcgtttgt tcacccggcg cgaagggatg
      481 cagccgagcg aactccggcc aggtcaccgg cagcatttca gcggtggcgt tcagcttcat
      541 cgtgcaggag cccaacggaa tcatcgagtg gacgagggac aggtctttag tctgcaaccg
      601 gtggagatag cggagcatct cgtgctcgga atgataacga tggaacacct cgtgcgtcag
      661 atatttactg gttctggcca aaggtgacga gtagctgaaa tcaatgtccg ccgcaagatc
      721 ggagagacgg aacggcaagt gatcatggcc gacaaaaatc cgtagtaaac gctgtacttc
      781 ttcctcggag ctgacttcgt cgagcgagac gccgatcgag ccgtcctcat aagacctgaa
      841 attgatccct tgttcgttcg cccgcgttgc tatgtgatct gcttgagcct tgggtactga
      901 tacccgaatg gtatcgaaga agactttcgg taagacgtcg aacccaagtc ggcgcagacc
      961 ttcagccaac agcaacgtga ggccgcgtac acgttcggcg atccggcgca acccgtccgg
     1021 cccgtggtac accgcataca tggcggccat gacggccaac aagacttgag ccgtacagat
     1081 gttactggta gccttctccc gtcgaatatg ttgctccctc gtctgaagcg aaagcctaat
     1141 ggccgattgg ccggtcacat cttttgagat accaaccagg cgacccggca cctgccgttt
     1201 gtactcctct ctggtagcca gaaacgcggc atgagggcca ccaaagccga tcggcacacc
     1261 gaaccgttgt gtcgaaccga cggcaatatc ggcaccgaat tccccgggcg aacggagcaa
     1321 ggtcaaggcg agaagatcgg tggcaaccgc cactcgaaca tcggcctcat gagcctgcgc
     1381 gaccaatgtg ctgaaatcac ccacgtagcc gtctgtagcc gggtactgca acagaatgcc
     1441 gcacagctga ggactcgaac aatcaacgga ggaggcgaca ccggtcttga tgacgatacc
     1501 caaaggttcg gctctggtct gcaacaccgc caaggtctgg ggatggcaat cgcgcgagac
     1561 aaaaaattcc tttcgttcct cgcccgcgtt gcgggcgatc gtgtaacaca tcgccatcgc
     1621 ttcagctgca gcggtcgctt catccaacag cgaggcattc gccagcggca gaccggtcag
     1681 gtccgccacc atagtctgga agttcacgag tgcctctaag cggccttgtg aaatctctgc
     1741 ttgatatggg gtgtattgcg tataccatgc aggattttct agaatatttc gctggatcac
     1801 gcccggggtg acgcaattgt agtaccccat gccgatcagc gatcgataga ctttattttg
     1861 agacgcgatg cctttcaggc gggccaggac ggcctgctcg ccgtcgccgt ccggaatgtc
     1921 cagagaccga cgaaaacgaa ggtcgggagg aatggccgta tcggccaacg tatcgagcga
     1981 cagtagaccc aacgaggcca gcatttcctg aatatcggcc cttgtgggac ccaggtgccg
     2041 gtgaacaaag tcatcggtcg gttggagcca attctgagtg atcatggagc cggctgtccc
     2101 tccgtttggt tcgcggggac ggtgagaggt atccccgtca attgggcacc gtaccacgct
     2161 gtcggctctt tttccaagag accggatccg ttttcgggga tgatttcatg gtggtcatga
     2221 taatagatag gtgggttgcg agggccttgt ctgaagcagc cgtcgggctc ggctgcgcac
     2281 gggtaaatcg tcttgcagag gggccggctt tggttgcttt tcacataatt cacgctattg
     2341 ttagctcata cctccagtcc gg
//
LOCUS       DATNDD010000064         7471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3845296, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000064 DATNDD010000000
VERSION     DATNDD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3845296"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="VN647_01385"
     CDS             complement(<1..1593)
                     /locus_tag="VN647_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWG95707.1"
                     /translation="MKRTFDVCMAALGLILTAPLLALISVLIKLDSQGPVIFRQVRVG
                     QGFRPFAIQKFRTMAVEVLGTCLPLTVGQDPRITRIGRILRKFKLDELPQLFNVLVGD
                     MSFVGPRPEVPRYVERLRSEFFEVLTVRPGITDLASLRYIDEAALLAYSASPEEEYQL
                     KVLPEKLRLAKLYIRHMSLRFDLAIILQTLLHIARLPVVVFTLPELKAAVETPVASPW
                     MSLSSFIMRWRKPIIVVLDVILIILANYFAFVLRYDGSIPEGDIQTFEQTVLALVAIR
                     GVAFALFGLNEGLWRYTSIWDLQNIIGGVLTSTVVFYGWVHWVMDLYSYPRSIFAIDA
                     ILLVGFLAGVRLSSRVLRDKPIFQKKRKVFIVGAGDAGERVVREMKTRTVFNCQPIGF
                     VDDNVGLLNQRIHGVRVLGTVQDLPKLVEGLKPEVVVVAVPNAAPEFLRDLVIKLEPY
                     NISIKTLPAKEELLSDQSTVSQIRNVSIPDLLSRAPIHLDNEATRHMVRGKCVLITGA
                     GGSIGSELARQITVFEPTVLLLY"
     gene            complement(1593..2750)
                     /locus_tag="VN647_01390"
     CDS             complement(1593..2750)
                     /locus_tag="VN647_01390"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWG95708.1"
                     /translation="MKDFLPFHRSDVGEEEVSEVLEVLRSGWLTTGPKVREFEREFAA
                     MVGAEHAVAVNSCTAALHLALEAAGVREGDEVLVPTMTFAATAEVVTYFKARPVLIDC
                     VQDTLNLNTDRIQQAITDKTRAIIPVHFAGHPCDLRPIQTIAQDHNLHVIEDAAHALP
                     ARYHGKMIGGISDATCFSFYATKNITTGEGGMVTTNNAEWAARMRMMSLHGLSRDAWN
                     RYSAQGSWYYEILSPGFKYNLTDIAAALGLAQLKKCERFWKGRERYAALYQEGFQDVP
                     EILCPPTASHVQHAWHLYVIQLNLDCLRISRDEFIRQLQHAGIGCSVHFIPLHLHPYH
                     RDRGGYRPEDFPVASMVFQRIISLPLYSKMTEDEINRVIETVRDLVKRNRR"
     gene            3895..5475
                     /locus_tag="VN647_01395"
     CDS             3895..5475
                     /locus_tag="VN647_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95709.1"
                     /translation="MIMIRDITLLAIFSIIYLQAPVAQSATYYISPTGSDTNPGTSAK
                     PWLTFAYAINSARAWCGDTLTLLNGTYGDGTSTGKITVSGVVCTAGHELMIVAANQRQ
                     AKINDNGSGRAVYVLNSAYIIIDGLYARSTDNSGSTSSGYPFFTNRNNHITLKNLVAR
                     NPNRYANTHTIAVENSQDVLLEDNEAYVFHRHCVEAWVSERVVVRRQYCNPRGGRIAG
                     GFGEANGLGKADALVTMYPCRDCIQENTIADGTTNPMYLNEMNATYGAGILMSGSKVL
                     GSICYKCDYGNGIYINSRKVADLNHTPQNVTIRDVAFVDWNSNANGLRVTDGVNILLD
                     HITLLSTGGGVNGVIAEDTANGSTPTTNSITMTNMLIAGLTTGTGFRVSNYNTWSGDK
                     LFSNGNRAAFEASPPSNLTNTSTKAHGMGTCKVWVPAGAAIRGIGTGGSDIGANILYR
                     YINGVLTSTPLWNPKTGEFPHGAPDLDETNRVAGQSLFDIHTRLNVNTGGCSFPRNYG
                     NDDSDTTLPAAPVGLRAS"
     gene            complement(5680..6816)
                     /locus_tag="VN647_01400"
     CDS             complement(5680..6816)
                     /locus_tag="VN647_01400"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWG95710.1"
                     /translation="MGVPLLDLKAHHEPLHQEIMAALEQTFHSQAFILGPDVGKLEER
                     VATYCQAKYGIGVSSGTDALLIALMALGIGPGDEVITTPYSFFATAGAVVRLGAKPVL
                     VDIDPITYNIDTAKIKSVLTAKSRAIIPVHLYGQCADMAPIMDIAKRHNLSVIEDAAQ
                     AIGSEYYDGRRACSIGTIGCLSFFPSKNLGCLGDGGMVVTSDADLAERIRVLRVHGSK
                     PKYYHKRVGGNFRLDTIQAAVLNVKLNYLDGWTKRRQENATRYETLFKQSGLTQNGKV
                     KLPEPVYRNSGAKHYHIYNQFVLRVEQRDVLMAHLKQKGIGAEIYYPVPFHLQECFLY
                     LGYREGDFPQSERAAKETVAIPIYPELTAEQQTEVVEAITAFYR"
     gene            complement(6832..>7471)
                     /locus_tag="VN647_01405"
     CDS             complement(6832..>7471)
                     /locus_tag="VN647_01405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWG95711.1"
                     /translation="VLQRCQEALEQAGPRMTPPVPKPDKPYFAHESAFVDEGVEIGGG
                     TSIWHTSHILKGSRIGKNCKIGQNVVVGPNVMVGNGVKIQNNVSVYEGVTLEDHVFCG
                     PSMVFTNVFNPRSEIPRMKELKQTLVKRGATLGANSTILCGITIGRYAFIGAGTVVTK
                     DVPDHALVVGNPSRITGWMCLCGVKLHVKGENAACPACGQQYRAEQAGMMVV"
BASE COUNT         1953 a         1949 c         1771 g         1798 t
ORIGIN      
        1 gtagagcaat agcactgtgg gttcaaacac ggtaatttga cgcgccaatt ctgaaccaat
       61 cgacccgccg gctccagtaa tcagaacaca cttacctcta accatgtgtc ttgtggcttc
      121 attgtctaga tgaatgggcg cacgcgacaa aagatccggg atcgaaacat tgcgtatttg
      181 gctgactgta ctttgatctg aaaggagttc ctctttagca ggcagtgtct tgattgagat
      241 attatacggc tccaatttga taacgaggtc acgaaggaac tcaggagccg cattcggtac
      301 cgcaaccaca actacctcgg gcttgaggcc ttctacaagt ttggggagat cctgcaccgt
      361 acccaatact cggactccat ggatacgttg attaagaagg ccaacgttat catcaacgaa
      421 tccaatcggt tggcagttaa acaccgtacg agttttcatc tcccgaacga ctcgttctcc
      481 tgcatctccg gcaccgacga tgaaaacctt ccgttttttc tgaaaaattg gcttatcgcg
      541 aaggaccctt gaggacaatc tcaccccggc aagaaacccg acgagcaaga tagcatctat
      601 ggcaaatatg gaacgcggat aactatagag gtccatcacc caatgaaccc aaccataaaa
      661 tacgactgta cttgttaaga ccccacctat aatattttga agatcccaaa tgcttgtata
      721 cctccacaaa ccttcgttta ggccaaacaa ggcaaatgcc actcctcgaa tggcaaccaa
      781 tgcaagtact gtttgctcga aggtttggat gtcgccctca gggatactac cgtcatatcg
      841 caggacgaat gcaaagtagt ttgccagaat tattaagatc acatcaagaa cgacaatgat
      901 tggcttccgc cacctcataa taaaggacga tagactcatc caaggagagg ccacgggagt
      961 ctcgacggca gcttttagtt caggaagtgt gaagaccaca accggaagcc tagcaatatg
     1021 cagcaatgtt tggagaataa tagcgagatc gaaccgcaga gacatatggc ggatgtatag
     1081 ctttgccaac cggagctttt ccggaagaac tttgagttga tactcttctt caggacttgc
     1141 ggaataggcg aggagcgcag cttcatcaat atatctcaac gaagccaagt ctgtaatccc
     1201 ggggcgaacc gtcagtacct cgaaaaactc agaacgtaag cgttctacat aacgtggaac
     1261 ttcaggcctt ggaccgacaa agctcatatc accgacaaga acattgaaca gctgaggcaa
     1321 ttcatcgagt ttgaatttcc gtagaattcg accgatcctc gttatgcgag gatcttgtcc
     1381 aactgtaaga ggcaagcagg taccaagaac ttccacagcc atggttcgga atttttgtat
     1441 cgcgaaggga cgaaaccctt gtcccacgcg aacctgacga aagatcaccg gtccctgcga
     1501 gtccaacttg atcaaaacag agatgagtgc taatagcggc gccgtgagaa tcagccctag
     1561 ggcagccatg cagacatcaa acgtgcgttt catcaccgtc ggttcctctt gacaagatca
     1621 cgaacggtct caatcacacg attgatctcg tcctcggtca tcttcgagta caacggcaat
     1681 gaaatgatcc gttggaacac catactggca acggggaagt cttcaggtcg gtagcctccc
     1741 ctatcccgat gatacggatg taaatggagg gggatgaaat gcacactgca tccgataccg
     1801 gcgtgctgga gttgacggat gaattcatcg cgactgatcc gcaagcaatc caaattcaat
     1861 tgaataacat acagatgcca tgcgtgctgt acatgagaag cagttggagg acacaagatc
     1921 tccggaacat cttgaaaacc ttcctgatac agagcggcat accgttcacg ccctttccaa
     1981 aaacgttcac acttcttcag ttgggccaaa ccaagcgccg cagcaatgtc ggtcaaattg
     2041 tacttaaaac caggcgaaag tatttcgtaa taccacgaac cttgcgcaga gtaacgattc
     2101 caagcatctc gactgagacc gtgcaaactc atcatccgca tacgagcggc ccactcagcg
     2161 ttattagtgg tcaccattcc gccttcgccg gtggtgatat tcttcgtcgc gtagaacgaa
     2221 aaacaagtgg cgtcagaaat cccgccgatc atttttccat gatatctcgc tggtagggca
     2281 tgcgccgcgt cttcgatcac gtgtagatta tgatcctgtg cgatcgtctg aatgggtcgc
     2341 aggtcacacg gatgcccagc aaaatggaca gggatgatcg ctctcgtttt atccgtaatg
     2401 gcctgttgaa tacgatccgt attcagattc aacgtgtctt gtacacaatc gatcagaact
     2461 ggtcgtgcct tgaaataggt aacaacctca gctgttgcag caaaggtcat cgttggaact
     2521 agtacctcat ctccctcacg cacaccagcc gcctcaaggg caagatgaag ggcggccgta
     2581 caggaattaa ccgcaacggc gtgctcagcc ccgaccattg cagcaaattc acgctcaaat
     2641 tccctcacct tgggacccgt tgttaaccag ccggagcgaa gaacctccag cacttccgac
     2701 acctcttcct ctccaacatc agacctatga aaaggcaaaa agtctttcat tcacagatct
     2761 ccctctttgg ctaacgaacc tgtccttatc agggctacac tttcccataa ttccggaaaa
     2821 gcacaaccca tctttggagg ggccatggaa actatctatt taagaaaaat gctcaaaaaa
     2881 tcaaacgatt accaatccta caatatttcc tacaatcttg aaaagaccat agctattgga
     2941 gaatattaaa ttagaatatt ataatttttc accgggagag tgatagacgg aagttccttg
     3001 cagacatcga gatagcctct tcccttaagg aacggagtac tatacgcaat acagcccact
     3061 ctctttcttg acaattagag ctaatcgttc tgacgtttag caggcttcgg aaaaactcgc
     3121 tcgttcatct cgttcattgg gtgaaactcg tagaaagcac gatttccttc tggacaagtg
     3181 aagttttgcc tcagcgtggc agttcccctg ttctggtcga agcaaatgcg gttttcgagc
     3241 aagtcagcag tcattccaga catcgaagtt gacgcttcgg aagagagacc ccaaaaacat
     3301 ttttccgcag cctgttagag ctaatcgttc tgacgtggat cctattccaa gatcactccc
     3361 aacagaactt ggctgttggg atagtttgac agcccttcag agaaaaaagg agggcatgaa
     3421 agtcggcttt tagagagtag attgccgcgg ccttgctgga ggttgatgcc ggcgccccaa
     3481 ttcaggtggt cacttggatc ttaagcatca ataagctaca ttctggtgta tggcagataa
     3541 ggtatggtta gatctccgcc gggtactgca gctagaagac tagaagataa gaattgcaga
     3601 ctgaagcggc ggccagaatt tccgtaggtc taagagatct caatccagaa tacttaattt
     3661 caggagcgca tgggagaaca ttaatgtact aatgcacttt tttgttcgct gctttgccgt
     3721 aacagtagca gctctgatta cttttgttat tctccctcat aaatgttgcc ggtttaagga
     3781 agttgcctag atccaagaaa atccgcatcg gacatggcac agattatgcg tccaaacaac
     3841 tcggcgattc ctggaacatc ttggcgatgt ggtcttgatt aaagaggcag gttcatgatc
     3901 atgatacgag acatcacgtt gttggctatc ttctcgatca tctacctcca ggcacccgtt
     3961 gctcagagtg cgacctatta catttcacca acaggatccg acaccaatcc cggtacttcc
     4021 gcaaagccat ggcttacctt tgcgtatgcc atcaattccg ctcgcgcttg gtgcggcgat
     4081 acactgacac tgcttaatgg gacgtatgga gatggcactt ccaccgggaa aattactgtc
     4141 tctggagtgg tctgtacagc aggtcacgaa ctcatgattg tggcggccaa tcagcggcaa
     4201 gccaagatca atgacaacgg ttctggacgc gccgtctacg tccttaatag cgcctacatc
     4261 atcattgacg ggctgtatgc acgatccacc gataacagcg gctcgacatc gtcaggctat
     4321 ccatttttta caaatcgcaa taaccacatc acgctcaaga atcttgtcgc tcgcaatcca
     4381 aaccgctatg ccaataccca cactatcgcc gtcgaaaatt cacaggatgt gctgttggaa
     4441 gacaatgaag cctatgtctt ccatcgtcac tgtgtcgagg cctgggtaag tgagcgcgtg
     4501 gtcgtgcgcc ggcaatattg caatccgcgt ggcggtcgga ttgctggcgg gtttggcgag
     4561 gccaatggac tcgggaaagc ggatgccttg gtgaccatgt acccctgccg cgattgcatc
     4621 caagagaata ccatagcgga cggtacgacc aatccgatgt atctgaacga gatgaatgcg
     4681 acctatgggg ctggtattct catgagtggg tcaaaggtgc tgggatcgat ttgctataaa
     4741 tgcgactatg ggaatgggat ctatatcaac tcgcgtaagg ttgcggatct caatcacaca
     4801 ccacagaacg tcaccattcg agatgtggct tttgtggatt ggaattcgaa cgcgaatggt
     4861 ctcagggtga cggatggggt caatattcta cttgatcaca ttactctcct gagcaccggt
     4921 ggcggggtta atggggtcat tgctgaggat acagcaaacg gatcaacgcc tacgacgaac
     4981 tctatcacga tgacgaatat gctcatcgct ggcctgacta ccggcaccgg ctttcgtgtc
     5041 tccaattaca acacttggag cggcgacaaa ttgttctcaa acggaaatcg ggctgcattc
     5101 gaagcatcac ccccgtcgaa cttgaccaat acctcgacca aggcccacgg catgggaacc
     5161 tgcaaggtct gggtgccagc tggtgctgcg ataaggggta taggaacagg tggtagcgat
     5221 attggcgcga atatcctcta tcgatatatc aatggggtgt tgacctcgac tccgctctgg
     5281 aaccctaaga ccggagagtt tcctcacggt gcgccagacc tcgatgagac gaaccgtgtc
     5341 gcaggacaat cgctgttcga tattcatacg cgccttaatg tgaacacggg agggtgctca
     5401 tttcccagaa attatggaaa tgacgattcc gatacgacac taccagctgc accagtggga
     5461 cttagagctt cctagcgatg atcggcttcg aagatactat ttaatcgctg ttgttaatgt
     5521 atgtgttctt cctgcaatca gccaactcgt caaacatatg aacatcctgc tggtagaccg
     5581 ttctctctga gcgacgctga agtgcttaat ccatattttc accagtgcta ttctgctccg
     5641 ggaaatactg cctcagaggc gatcggtcgc agctatctgt tatctgtaga aggcggttat
     5701 cgcttcaacc acctcggtct gttgttcggc agtcagctcg ggataaatgg gaattgcgac
     5761 agtctccttg gctgcacgct ccgactgcgg aaagtctcct tctcgataac ctaaatagag
     5821 aaaacactct tggaggtgga acgggacagg atagtagatt tctgcaccaa ttcctttctg
     5881 cttcaaatga gccatgagca catctcgctg ctcgacccta agcacaaact gattataaat
     5941 gtgatagtgc ttagcaccgg agttgcggta tacaggctcc ggcaatttca ctttcccatt
     6001 ctgtgtgagc ccgctctgtt tgaacagagt ctcgtatcga gtggcattct cctgccgtct
     6061 tttggtccac ccatcgagat aattgagctt tacgttcaga accgcggcct gaatcgtatc
     6121 aagacggaag ttccctccaa cccgcttgtg ataatacttc ggcttgcttc cgtgaactcg
     6181 caggacccgt atgcgctctg caaggtcagc atcgctggtc acaaccatcc caccatcgcc
     6241 taaacatccg agattcttac tggggaagaa cgacaggcaa ccgatagtcc cgatactaca
     6301 ggctcgccgc ccatcatagt attccgaacc aatggcttgg gccgcatcct cgatgacgct
     6361 tagattgtgc cgtttggcga tatccataat tggggccatg tcggcacatt gtccatagag
     6421 atggaccgga ataatggccc tgctcttagc cgtcagcacc gacttaattt tggcggtatc
     6481 gatgttatag gttatgggat cgatgtctac cagcacaggc ttggctccca gtctcacgac
     6541 cgctccggca gtggcaaaaa atgaataggg cgtcgtgatg acctcatcac cgggaccgat
     6601 accgagagcc atgagagcga tcaggagagc gtcggtccca gaactcacgc cgatgccgta
     6661 cttggcctgg caataggtgg ccacacgttc ctctaacttg cctacatccg gtccgaggat
     6721 aaaggcctgg ctatggaaag tctgttccag cgccgccatg atttcctgat ggagcggctc
     6781 gtgatgtgct ttcaaatcga gtaatggaac acccatgacg atctcctttt cctaaaccac
     6841 catcatcccc gcttgttcgg cccgatattg ctgcccacag gccggacagg cggcattctc
     6901 gcccttgaca tggagcttaa ccccacacag acacatccag ccagtaatcc ggctgggatt
     6961 accgactacc agtgcatgat ccggcacatc cttggtcacg accgttcccg caccgatgaa
     7021 cgcatatcgc ccgatcgtaa ttccacacag aatcgtcgaa ttagccccca gcgtagctcc
     7081 tcgttttaca agcgtttgct tgagctcttt catacgaggg atctcgctcc ggggattaaa
     7141 gacatttgta aaaaccatcg aaggaccaca gaagacatgg tcttcaagcg ttacaccttc
     7201 atagacggat acgttgttct gaattttgac tccattgccg accataacat tggggccgac
     7261 gacaacgttc tgcccgattt tacagttctt cccgatgcgc gaccctttca gaatgtgtga
     7321 cgtgtgccag atgctcgttc ccccaccgat ctcgacgcct tcatcgacaa acgccgattc
     7381 atgggcaaag taaggcttat ctggcttagg gacaggaggc gtcatgcgtg gaccggcctg
     7441 ttccaatgcc tcttggcacc gctgcaacac g
//
LOCUS       DATNDD010000065         3514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1832695, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000065 DATNDD010000000
VERSION     DATNDD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832695"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..463)
                     /locus_tag="VN647_01410"
     CDS             complement(233..463)
                     /locus_tag="VN647_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95712.1"
                     /translation="MLSPQIPADKWWMRGVIARSVGIVAVALGFVLGMYGLDHPTSPW
                     LPTALGLLVTGMVAQGYGLYCSIKRVQQLNSR"
     gene            609..917
                     /locus_tag="VN647_01415"
     CDS             609..917
                     /locus_tag="VN647_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HWG95713.1"
                     /translation="MKLTRSAVERLKALIHEHPEDPIVRVQVRDLDEQRLSFSITLED
                     RVQSNDEVQIVDSLTVAIPSASALRMNGITMDYQEPDGFKFHHSDQQNDPLLNIISLN
                     "
     gene            1205..2320
                     /locus_tag="VN647_01420"
     CDS             1205..2320
                     /locus_tag="VN647_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG95714.1"
                     /translation="MESTETTSTQDLREKTDRSLEEERDKADEYLTQPFQEVEEKTHE
                     KVHSIRDAVDQAKESQRDALDDDKERFREIADASLSQVDDELLAEERERLDKLQEAER
                     EAVDRVRAEEHAQKESLVNAILEHERQQTDSHLLDERVHADRELITREQFLSIVSHDL
                     KNPLAAISISTRLMRKSLSKGETDSLLEHLGRIQHSADAMGRMINDLLDVERIAQGKL
                     TLHLAKVDLRALLQECSELFAPLVSNQAFSMRIDVGAEPLIVDADHDRILQVLSNVIG
                     NSLKFTPNGGAIELSACKRETHVEVSITDNGPGISAEAQPRIFERFSQLNVADRQGLG
                     LGLFIAKWIVEVHQGRIGVTSTPGNGSTFSFTLPLSV"
     gene            2610..>3514
                     /locus_tag="VN647_01425"
     CDS             2610..>3514
                     /locus_tag="VN647_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWG95715.1"
                     /translation="MTEEWGAIFVVDDDADMRELAYDMLKDRGHQVTMAGSGEEALRR
                     LAEEDYAVVLTDLRMRGMQGLELLTQIKRNHSDINVILMTAFGSVETAVEAMKHGASD
                     YLTKPVKKDELIRVVERVMREAALRREVGRLRREVRKEYSFYQILGKSKAIQAVFDLI
                     RRVADSPTNVLITGESGTGKELVAKAIHYNSDRNHAPFIPVNCAAIPEQLLESELFGH
                     MRGAFTDAKMDKRGLFEEAQKGTLFLDEISELPLMLQAKILRAIQEKEIRRVGATKPI
                     SVDVRIIAATNLNLSEEVKSKRFRE"
BASE COUNT          916 a          907 c          960 g          731 t
ORIGIN      
        1 aatccgcgca agaacccatg acgcgagaca tccccaagaa gggacttcag caacgccggc
       61 aggtccttca gagtattctg accgagctgc aggaactcga cgcgcaagaa gttgaggtgc
      121 tcacagggga agatcgtgag gaccctccgg gccccagcta ggccggccta agtctcctct
      181 tcgccgtgcg ccggcagacg accgcctcaa acgttccgag tcaatgaagg cctcatctgg
      241 agttcagttg ctgcacccgc ttgattgaac agtaaaggcc atagccttgg gccaccatcc
      301 cggtcacgag taacccaagg gccgtcggaa gccaaggtga ggtagggtga tcgagtccat
      361 acatgccgag gacaaatcca agagcaacgg cgacgatgcc gactgatcgc gcgatgacgc
      421 cccgcatcca ccacttatct gccgggattt ggggagacag catgtcttca gtctaccggt
      481 gaatggaaag agagtgcaat cgccaacgag cggacagcca ttacgtgcgt cggcattcca
      541 tgcaagagca ctgaagcggt catcggatta tttgtgatgt cgtggggtca aagagaaacg
      601 tgtagaccat gaaactcaca cgatcggccg tcgaacgact gaaggcgttg attcatgaac
      661 accccgagga ccccattgtg agggtgcagg tgagggacct ggacgaacag cggctgtcct
      721 tcagcattac gttggaggac cgggttcagt ccaacgacga agtgcagatc gtcgacagtc
      781 tgacagtggc gataccttcc gccagtgctc tgcgcatgaa cggcatcacc atggactatc
      841 aggaaccaga cggattcaaa ttccaccaca gtgatcagca gaatgatcct ctgctcaaca
      901 tcatcagctt aaactgatgc ggcatttaga agttctggtc ctgccgcgct gccattcggc
      961 tgtggcgggc tctgcaaaca gcgcgcgcct tcctaagcct ttttcttgcg ctgatacttt
     1021 tcgtgaactt accacatgag cgtaatctag aataaacgct tgcaggcgaa gctcgccgcg
     1081 ttgttgacat gttggaacga gggcacctac tatactgcgc cgccaagaaa gctgaaacat
     1141 cctagttccc tacattgatc tgattccgcc attgccagag catcaaaagg agaggtctcg
     1201 atccatggag tctacggaaa caacatccac tcaagacctg cgagagaaga ccgacaggag
     1261 cctagaggag gaacgcgaca aagctgatga atatttgacc caaccgtttc aagaggtcga
     1321 ggaaaagacc catgagaaag tccattcgat tcgggatgcc gttgatcagg ctaaagaaag
     1381 tcaacgcgat gccctggacg acgacaaaga acgcttccgt gaaatagctg atgcttccct
     1441 ttcacaggta gacgacgaac tgttggctga ggaacgcgaa cgacttgaca aattgcaaga
     1501 ggctgaacga gaagccgtag atcgcgttcg tgcagaggaa cacgctcaaa aagaatcgct
     1561 cgtcaacgca atccttgaac atgagcgcca acagaccgac agccatctcc tcgatgaacg
     1621 tgtccacgca gacagagaac ttattacaag ggagcaattt ctctccattg tcagtcacga
     1681 tctaaagaac cccctcgccg ccatttcgat cagcacgcgt ttgatgcgaa aaagtctttc
     1741 taaaggcgag accgacagtc ttctagaaca ccttggacga atccaacaca gcgccgacgc
     1801 catgggcagg atgataaacg atctgcttga cgtagaacgg atagcacagg gcaagctgac
     1861 acttcacctt gcaaaggtcg accttcgagc tctgttacaa gaatgtagtg aactcttcgc
     1921 tccactcgtg tcaaatcagg cgttttccat gaggattgat gtaggtgctg agcctctaat
     1981 cgttgatgcc gatcacgata gaattcttca agtgttgtcc aacgtgatcg gaaattcact
     2041 gaaatttacg cccaatggag gcgccattga gctctctgca tgtaaacgag agactcatgt
     2101 tgaagtctcg ataaccgaca acgggccagg gatctcagcg gaggcacagc cgcgaatttt
     2161 tgaaaggttc tcgcagctca atgtggccga ccgtcaggga cttggccttg gtctttttat
     2221 cgccaaatgg attgttgagg tccatcaggg acgtattggg gtgacatcta ctcccggaaa
     2281 tggatccaca ttcagcttca cgcttccatt atctgtctag ggcgccgcca tcaacttcag
     2341 tctgcaagga gtcggctatg gcctatcttt agcggcttca gcggctcgtc cctcgatccg
     2401 gctgcaggtt catcacttcc ggttttagaa atttcctctt tggctttttc tggccctacc
     2461 cttgccttca ccctatgtat ggatgccgag cagactcaag atggggcata attcatcaac
     2521 taacgcgggc agaccgtggc aatctgggac aacaagttca ggctcacttc aagacaaact
     2581 tatcctcttt caatgttcat aggtgctgca tgactgaaga atggggggcc atctttgtcg
     2641 tggatgacga tgccgatatg cgggaattgg cctatgacat gctgaaagac cgaggtcatc
     2701 aggtaaccat ggcaggaagc ggtgaggagg ccttgaggcg attggccgaa gaagactatg
     2761 ccgtcgtcct gacggatctt cgcatgagag gcatgcaggg gcttgaattg ctgactcaga
     2821 tcaaacgcaa ccattccgat atcaacgtga ttctcatgac cgcttttgga tcggtggaaa
     2881 cggcggtcga agccatgaaa catggggcga gcgactacct taccaagccg gtcaagaaag
     2941 acgagctgat ccgcgtggtc gagcgggtga tgagggaagc ggcattacgg cgggaagtcg
     3001 gccggctcag aagagaggtc cgcaaggaat acagctttta ccagatattg ggaaagagca
     3061 aagcgatcca ggcggtgttc gatctcattc ggcgggtagc cgatagcccg acgaatgtgc
     3121 tcatcacagg agagagcgga accgggaagg aattagtggc gaaagccatt cactacaaca
     3181 gtgacagaaa ccatgctcct tttataccgg ttaactgtgc ggcgattcca gagcagctct
     3241 tggagagcga actgttcggg catatgagag gcgcctttac cgatgcgaag atggacaaac
     3301 ggggattgtt cgaagaagcg cagaaaggca ccctctttct cgatgagatc agtgagctgc
     3361 cgctcatgct tcaagccaaa atccttcgcg cgattcagga aaaagaaatc cggcgggtgg
     3421 gagcgaccaa gccgatctcg gtggatgtgc gcatcatcgc ggccaccaac ctgaatctca
     3481 gtgaggaagt gaagagcaag cgattccgcg agga
//
LOCUS       DATNDD010000066         5532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2698232, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000066 DATNDD010000000
VERSION     DATNDD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698232"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VN647_01430"
     CDS             complement(<1..1198)
                     /locus_tag="VN647_01430"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWG95716.1"
                     /translation="MILLWSCRYAEIRRLFRFVIVAVLLWEAVPALSVHAETMIVPTL
                     SVRSWYDTNVYRRPKQLLTPGTQAEDFVTSVLPVLDVLHKTRDIEADVKMGGIFTTYV
                     ENTSRNYFGATLQGGVRLDHWVEQYVRGARLQVVENLRYTPEQPSFLQGSRDLPTDAD
                     LTTGVQGFRANTLYNTTQVTGGYPVSRDLSMEGGYTFVIRRIGNVQGGLGPIAGSTIY
                     FDTMAHTWFGGPRYHLTRNDSVAVQYKQTFITQSQAQGGRNFNTNLIALEGDYTKEFQ
                     EWRFKAQGGITFAEPVGRAFPSGKLELTNQLERDTVVRLTVSRQGMPSFFLQGGAMIT
                     NLASLGISHRIYERLILDGTVGYAYNQYFPQTDKTIKNMTARATISYKLTRAITGQIF
                     YIFQNID"
     gene            complement(1329..1952)
                     /locus_tag="VN647_01435"
     CDS             complement(1329..1952)
                     /locus_tag="VN647_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWG95717.1"
                     /translation="MRTADLSKMRTLSIGLLLCSFAIQAGCLGPVTSREYKSSDVPTE
                     FLLGSEDLIEINVWKNPDLSRITLIRPDGYVSMPIIGDIQASGLTAEALAAQITERLK
                     GYIQNPSVSVNVKELNSYSVFVLGEVTKPGKYQLKSYVTVLQAISMAGGFTNYASKNR
                     LQVVRVIESPGHKRQEIHIPLRYDDLVSGRGEPGNIVLASGDTVVVP"
     gene            complement(2001..2780)
                     /locus_tag="VN647_01440"
     CDS             complement(2001..2780)
                     /locus_tag="VN647_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWG95718.1"
                     /translation="MYNLNVGFGAGAVPTEGTMIMKYSLWVLFISLALAVSSGPGFCD
                     SGSVKPSGAGKLPTDTTVLPAATPQTEKAISQVVMDKLSNAVSSEYVIGAEDMLDITV
                     WRNPDLSRQVQVRPDGRISMPIIRDVVAVGKTPTQLAEEMTNKLKEYVQNPVVAVTLK
                     EVNSSNVFLLGEVAHPGRYPLKSKMTLLQGITIAGGFKETAARNQIVIFRFIETAPGQ
                     KRFTASYDDIVLRSGISDNFELKPGDTLVVPSESMVVFPGR"
     gene            complement(2916..3764)
                     /locus_tag="VN647_01445"
     CDS             complement(2916..3764)
                     /locus_tag="VN647_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95719.1"
                     /translation="MRGETNRRKEVVAVLPAAGRSLRLVPLPCSKEILPVGLMTMTGL
                     RGARLRVVSHSLLECLQKADIRKAYIVIRQGKWDIPAYWGSGGMLGVNLAYMVIEDSS
                     GPPDTIDRAYPFVKDKIVAFGFPDIILRPKEVFAKLLDRLNCSGSDVVLALFPAHDAK
                     VMDMIDIDAALRVRAIHLKPRVTRLRYAWLCAVWTPVFTDFLHQFLRRIKQGEKAGVV
                     GNRRIDAQGDIPVGVVLKEAVKANLKVDGVTFPTGRYIDVGTPQALPMIRRFVSHARP
                     SYVTVK"
     gene            complement(3796..4728)
                     /locus_tag="VN647_01450"
     CDS             complement(3796..4728)
                     /locus_tag="VN647_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95720.1"
                     /translation="MNTIYFDPPFSDERRREELFQGQLLVYSPKRSTLAFIEFARKLI
                     EEAFAPYDPETAQRHLSVEQYADLLGKLKPNFIHHSQSKALMGDIFDEMGCDIEKTYF
                     DVPKMRSSTSDNYLTTGIAYAWHPHRDTWYSAPPCQINWWIPIYDIQSNNAMAFHPPY
                     WNVPVKNSSKGYNYYVWNQQNRGAHVARFLKEDPRPLPKPTESLVLDPQIRLIVPAGG
                     IILFSAAQMHSSVPNTSGKTRFSIDFRVVNLDDVAGRKGAPRVDEECTGTTMRDYLRA
                     SDLSHITADLVSLYDDETVDAGALTYQHKAEHSI"
     gene            complement(4742..>5532)
                     /locus_tag="VN647_01455"
     CDS             complement(4742..>5532)
                     /locus_tag="VN647_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HWG95721.1"
                     /translation="EILVSCHVCHPSMCNDNLSGITVTVKLAETIATCPRRYSYRFLF
                     IPGTIGSITWLAQNEQVVSRIKHGLVLTGIGDVGNITYKKSRQGNAEIDRAMGHVLRH
                     SGEAATIIDFFPYGYDERQYCSPGFNLPVGCFMRAPHGQYPEYHSSADNLDFVKQESL
                     VQSYARCLEVLELLEGNRTYMNQNPKCEPQLGRRGLYRAVAGQQEKQWTELALFCVLN
                     ASDGHHTLLDIAEHADLPFGKIQSAAEALLAVGLLKECRRPGDT"
BASE COUNT         1369 a         1535 c         1268 g         1360 t
ORIGIN      
        1 tgtcaatatt ttgaaagata taaaagatct gtcctgtaat ggccctggtc agcttatagg
       61 agatcgtcgc tctcgccgtc atatttttaa tcgtcttgtc cgtttggggg aagtactgat
      121 tataagcata gccgactgtc ccatcgagga tgagtcgttc atatattcta tggctgattc
      181 ccaggctggc aagattggtg atcatcgctc caccctggag gaaaaacgac ggcatgcctt
      241 gccgtgaaac ggtgagacgg accacggtgt ctcgctcaag ttggtttgtc agctcaagct
      301 tgccagacgg aaaggcccgc cccaccggtt cggcaaacgt gatccctcct tgagctttga
      361 aacgccactc ttgaaactcc ttggtatagt cgccctctaa cgctatcaga ttggtattaa
      421 aattcctgcc accttgggcc tgtgactgcg taataaaagt ttgcttatac tggaccgcaa
      481 cactgtcatt tcgcgtcaga tgataacgag ggcctccgaa ccaggtgtgc gccatggtgt
      541 caaaatagat ggtgcttccc gctatcggtc ctaagccccc ttgaacattc cctatcctcc
      601 gaatcacgaa tgtatacccc ccttccatag agagatctcg agacacagga tatcccccgg
      661 tgacctgcgt ggtattgtaa agcgtattgg ctcgaaatcc ctggacaccg gtagttaagt
      721 cggcgtctgt gggtagatcc ctcgatcctt gcagaaagct cggttgctcc ggggtatagc
      781 gcaagttttc cacaacctgc aaccttgcac ctctgacata ctgctcaacc cagtggtcca
      841 atctaacgcc accttggagc gttgcgccga aataattccg gctcgtgttt tccacatagg
      901 tggtgaagat tcctcccatc ttcacatccg cttcaatgtc tcgcgtctta tgcaacacat
      961 ctaggacagg aagcacactc gtcacaaaat cttcggcctg tgtgccaggg gttaataact
     1021 gctttggcct acgatacaca ttggtatcgt accatgaccg cacgctcagc gtcgggacaa
     1081 tcatcgtctc tgcatggacc gataacgccg gcacagcctc ccacagaagc accgcaacaa
     1141 tcacgaaccg gaacaaccgt ctgatttcag catacctaca agaccataat agaatcacta
     1201 acactccttt tcttgggatg gctgctcacg gatgaaggtc gcggctcacc ggtccacttg
     1261 ttctaccgcc tgcatgagac ttgaggaaaa cagagccagc gatacctcgg acattctgca
     1321 tgattccgtc acggtacgac gaccgtatcg ccggaggcca gcacgatgtt acctggttca
     1381 ccgcgaccgc tgacgagatc atcataacgc agcggaatgt ggatctcctg acgtttgtgt
     1441 ccggggcttt caatcacccg cacaacttgc aaccgattct tgctcgcata gttggtgaag
     1501 cctcccgcca tggaaatagc ttggagcacc gtgacgtacg atttaagctg atacttaccc
     1561 ggcttcgtca cttcccccaa cacaaacacc gaataactgt tgagctcttt gacgttgacc
     1621 gataccgaag gattctggat atagcccttg aggcgctcgg taatttgcgc ggcgagagcc
     1681 tctgccgtaa gaccggaggc ctgaatgtca ccgatgatcg gcatcgatac atatccatcc
     1741 ggccgtatca aggtaattct tgacagatcc ggattcttcc agacattgat ttctattaga
     1801 tcctcagagc cgagaagaaa ttcggttgga acatcggagg atttatattc tctcgatgtc
     1861 actggcccta gacaaccggc ttggatagca aaggagcaca acaagagccc aatcgataga
     1921 gttctcatct ttgacaaatc tgccgtgcgc atccttgtcc atctcgcgta attgtccacg
     1981 gtaatccttt ccggtattcc ctaccgacca gggaagacca ccatcgactc actcggaacc
     2041 acaagtgtat caccgggctt gagttcgaaa ttatcactga ttccactgcg gagcacgatg
     2101 tcgtcatagc tggccgtaaa tcgcttctga ccgggagcag tttcgataaa ccggaaaata
     2161 acgatttggt ttcgcgccgc cgtctctttg aacccacctg cgatggtgat cccctgaagg
     2221 agcgtcattt tgctttttaa cgggtatctc cctggatgag caacttcacc caacaggaaa
     2281 acattggaac tattaacctc ttttaacgtc acagcaacga cgggattttg aacgtattct
     2341 ttcagtttgt tcgtcatttc ctcggccaat tgggtaggcg ttttccctac ggccacgacg
     2401 tcgcgaatga tcggcattga aattcgccca tcaggacgca cttgcacttg tcttgacagg
     2461 tccggattcc tccatacggt tatgtccagc atatcctcgg caccaatcac atattcgctg
     2521 ctgaccgcat tcgacaactt atccatcacc acctgactga tcgccttctc ggtctgcggc
     2581 gtcgcagcag gcaatacggt ggtatctgtc ggcaatttac cggcgccgga tggcttcacc
     2641 gaacccgaat cacagaatcc ggggcccgag cttaccgcca gcgctagcga gatgaagaga
     2701 acccacaggg aatatttcat aatcatagtt ccttctgttg gtacagcacc tgcgccgaac
     2761 ccaacgttca aattatacat cttctgagac tgcttcaggc atgcgagaat tatctgtcaa
     2821 tacgtggggg gccagtcgat gcaacgacgc ccgcctccct cttcccggtt agatcaatgc
     2881 gcgtatcctt ggggcagtga atcgggatgt cgcctttatt ttaccgtcac gtatgatggc
     2941 cgagcatggg atacgaacct gcgaatcatg ggaagagctt ggggagtgcc aacatcaata
     3001 taccgccccg tggggaatgt gaccccgtca accttgagat tggccttcac cgcctctttc
     3061 aatacaactc ccaccggaat atcaccctga gcatcgatcc tacgatttcc aaccacccca
     3121 gccttttccc cctgttttat ccggcgcaaa aactgatgca aaaaatccgt aaacacaggc
     3181 gtccatacgg cacacaacca agcatatcga agccgtgtca ctcttggctt tagatgaatc
     3241 gctcgaaccc gaagagccgc gtcaatatcg atcatatcca tgaccttggc atcatgagct
     3301 gggaacagcg ccaacaccac gtcgctccct gagcaattta accgatccag cagtttagca
     3361 aaaacttctt tcggacgaag aataatatcc ggaaagccga acgctacgat cttatctttc
     3421 acgaatggat atgcccgatc aatcgtatca ggcggaccac tcgaatcttc gatcaccata
     3481 taggccaggt tcacccctaa cattccccca cttccccagt aagctgggat gtcccatttg
     3541 ccttgccgaa ttacgatata ggccttccga atatcagctt tttgaaggca ttcaagcaga
     3601 gagtgactga ctaccctgag gcgagccccc cgaagcccgg tcatggtcat taaaccgaca
     3661 ggaagtatct ccttgctgca gggaagcggc accaatcgaa gagatctgcc agctgctgga
     3721 agtaccgcca ccacttcttt gcgtcgattt gtctcacccc tcatcctgat cacttgaaat
     3781 caaacttcac acacatcata tgctgtgttc cgctttatgt tgatatgtga gcgcaccggc
     3841 atcgacagtt tcatcgtcat agagtgacac aagatcagcg gtgatatgtg aaaggtcgct
     3901 cgccctcaaa tagtccctca tggtggtccc agtacattct tcatccaccc gaggagcacc
     3961 tttccttcct gctacgtcat caaggttcac cacacgaaaa tctatactaa accgagtttt
     4021 ccccgaagta ttgggcacac tcgagtgcat ctgggccgcc gaaaacaaaa tgatacctcc
     4081 cgccggcacg atgaggcgaa tctgtggatc gagcacaagt gattcggtcg gctttggtag
     4141 cggcctagga tcttctttta ggaatctagc gacatgtgct cctcgatttt gctgattcca
     4201 cacatagtaa ttatatccct tggaactatt ctttaccgga acgttccaat acggggggtg
     4261 gaaggccatg gcattgttgg actgaatatc atagatcggg atccaccaat tgatctgaca
     4321 aggaggcgcg gaataccacg tatctcgatg cgggtgccag gcataagcaa tgcccgtcgt
     4381 tagataatta tcactggtag agcttcgcat cttcggtaca tcaaaatatg tcttctcaat
     4441 atcacagccc atctcgtcaa aaatgtcgcc catgagcgct tttgattggg aatgatgaat
     4501 gaaatttggc ttcagcttcc caaggagatc ggcatactgc tcaacggaaa gatgtctctg
     4561 agctgtctcc ggatcataag gggcaaaggc ttcctcaatc aatttcctgg caaattcgat
     4621 aaacgcgaga gtactcctct tcggagaata cacgagtagc tggccttgaa ataactcttc
     4681 gcggcgtcgc tcgtccgaga atggagggtc aaaatagatg gtgttcatga tcgcttctcc
     4741 attaagtgtc tcccggtctc cgacattctt tcaataatcc aaccgctagg agcgcttcgg
     4801 ctgcggattg aattttgccg aacggaagat cggcgtgctc tgcgatatca agcagtgtat
     4861 ggtgcccatc agacgcgttc aagacacaga agagagccaa ttccgtccat tgcttttctt
     4921 gttgaccggc aacggcacga tagagccccc gccgccccaa ttgcggttcg cattttgggt
     4981 tttgattcat gtacgtacga tttccttcca atagctccaa cacctctagg catcgggcat
     5041 acgactgcac aagagactcc tgcttcacaa agtccaaatt gtctgcagag gagtgatact
     5101 ctggatattg gccatggggg gcccgcatga aacatccaac aggcagatta aaccccggag
     5161 aacaatactg ccgttcatca tacccataag ggaaaaagtc gatgatggta gcggcctctc
     5221 ccgaatgcct caacacatgc cccatcgccc ggtcgatctc cgcattgccc tgacgacttt
     5281 ttttgtaggt gatgttcccc acatcgccta tcccggtcaa gacaagccca tgtttaatgc
     5341 gagacaccac ctgctcattc tgagccagcc atgtaatcga tccaattgtc ccagggatga
     5401 aaagaaagcg gtatgaatat cgtcttggac atgttgctat ggtttctgcc agcttcacgg
     5461 tcaccgtaat acctgacaaa ttgtcgttgc acatagaagg atggcacaca tgacacgaga
     5521 cgagaatctc at
//
LOCUS       DATNDD010000067         5168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2873968, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000067 DATNDD010000000
VERSION     DATNDD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873968"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VN647_01460"
     CDS             complement(<1..1261)
                     /locus_tag="VN647_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWG95722.1"
                     /translation="MSKQSLWWVLLTVLLLLWGADRANAVEYGELVQKDPYPCASTAP
                     GISEQRSPEIPCGTWAFKSTEDPVFKFMRDTGWITNERYHTVSDQTGKNWIEPADAIL
                     NRRKDIDWNRYLKTGLHLPDWIDLGFENRTRFESYDHPWRAAQKAGNGATDFQIPMRS
                     RLRVGLGGNGPFRFLFEGQDSRSFDNGEPGSFRDTTMINEFDVNQLFGAVKVDNVLGT
                     GLRTDALFGRFTLDAGNRRLIARNDFRNTTNAFDGFLWQLSRPNAWRLKAFVTETVQR
                     DDVSLDTQNNKSLFWGTYFESQQFRWFNFDAYYMGLNDRRVATVTSHRTYNTFGGRLY
                     KDPKPGEFDYEIENAWQIGHKGVTKHFAHFEHLDIGYMFNLPWTPRMLFHFDYVSGDH
                     KPGDSQDEGFDTLFGARNFEYNITSMWG"
     gene            complement(1323..2504)
                     /locus_tag="VN647_01465"
     CDS             complement(1323..2504)
                     /locus_tag="VN647_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWG95723.1"
                     /translation="MRTRIKHHGNTVQFSNHLKSVRTKQGLSQGELANRAGITRQAVS
                     SIESNQYLPTTAVALRLASVLACRVEDLFSLAQAEEIIEGTLIGRFLQAETQTQLIRV
                     KVATVGKRTVVRPVADLGEQLSFAVPADGYITDTRHTPSGTSVRVTLSRDRQAIQQEI
                     SVAGCDPAIFLAGEYLRRHKDQTTVVGWPMGSMAALQALQRGEVHVAGLHLFDPITGE
                     SNLPFLRRHLKGSAYEVVTFATWEEGFLVRKGNPLSIRTAADLAQTAITLVNREEGSG
                     ARLLLDQRLRASGVESTQIQGYDRIVSSHVEVARSIDGRQADVGIAIRSAAQLFGLDF
                     VPLQAARYDLVVPKPYLQSHPTLAHLFDTIVRGPFRNELQALGGYDTRETGTVHALRV
                     V"
     gene            3071..4708
                     /locus_tag="VN647_01470"
     CDS             3071..4708
                     /locus_tag="VN647_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95724.1"
                     /translation="MRISGSSVLFAIMMISLTYISSAWAVEEGQFVKKDGKWEFYSGE
                     DPGLKYLYLKGLITEEEYSKGLKVIETKERLSKPNFTIDVNNGLNFRVGEKFLLKLRL
                     LTQVRYSYSRYNEAWGTIGDSRNPEILGGQVEYRAFRHQSDSSTFSAPRVQLQFLGHA
                     FDPDFRYNVSVALNQTEWDQEGGSGRANLLDAYIASWHIPWATVQAGQQRVWFNRALI
                     SSNATTSFADPMVVQSVFASNLQGSRDVGVTILSDEEKYRFNYAFGIWGGVGANLTRE
                     GTAVSQNVLPPTGGGGPNAGTTRTYNYDTRFLTGEMMYTIRLLYKIAGNPGYGQGDIL
                     NSRTPQAAIAFGYAYDPAQNYLSSIRSDIVDRAFRQRVVKAYNGRLLGGGIYDFHTYE
                     LDFIAKYRGWSIQAEGFFRDQIVRNADSQTRPFDLGTIPTSPLAVGPPVSLGDAWGWY
                     VQVGKYVIPRKLEVAVRYGILDPSTRQKQDLTKELGVAVSYSFDGTYNNRLIIDYSNI
                     TMGSGGRAPDRFPFENLPGFGRDLVENRFNVQYQFYF"
     gene            4745..>5168
                     /locus_tag="VN647_01475"
     CDS             4745..>5168
                     /locus_tag="VN647_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWG95725.1"
                     /translation="MIFTHTFSRIIVFIGITVFTGPVVLSAHAQSEVLTVAAASNLKD
                     VFRKILPLFEAQNHDISVRVIYAQPKTLVKQIEEGAPVDVLIPAQFEDIDQLEKKGLI
                     LQDTKQVYAHTALVLITNAAFPAPVGSIQDLETTPVRRI"
BASE COUNT         1226 a         1328 c         1389 g         1225 t
ORIGIN      
        1 gcccccacat gctggtgatg ttgtattcaa agttgcgcgc gccaaataac gtatcgaacc
       61 cttcgtcctg actatcgccc ggcttgtgat cgccgctgac atagtcgaag tgaaagagca
      121 ttcggggtgt ccagggcaga ttgaacatat agccgatatc gaggtgctca aaatgggcga
      181 agtgtttggt cacgcctttg tggccgatct gccaggcgtt ctcgatctca taatcgaatt
      241 ctcccggctt ggggtcttta tacaggcggc cgccaaacgt gttataggtg cgatggctgg
      301 tcacggtcgc cacccgccga tcgttcaagc ccatgtagta cgcgtcgaag ttaaaccagc
      361 ggaactgctg gctctcaaaa taggtgcccc aaaagagcga cttgttgttc tgggtgtcga
      421 ggctcacatc gtctcgttgg actgtctctg tgacgaaggc tttgagccgc caggcattgg
      481 gccgactaag ttgccacaga aacccatcga aggcgttggt cgtgtttcga aaatcgttgc
      541 gcgcaatcag ccgccggttc ccggcatcca gggtaaagcg tccaaagagg gcatcggtcc
      601 gtaaccccgt ccccaggaca ttgtccactt tcacggcgcc aaacaactgg ttcacatcga
      661 attcattgat catggtggtg tcccggaacg agccaggctc tccattatca aacgaccgcg
      721 aatcctgccc ctcgaacagg aatcggaagg gtccgttgcc gcccaagcct acacgaagcc
      781 gcgatcgcat gggaatttgg aagtccgtcg ctccgttgcc tgctttctgt gcggcccgcc
      841 aggggtggtc gtaggattcg aaccgagtcc gattctcaaa gcccagatcg atccagtccg
      901 gcaggtgcag tccggttttg aggtagcgat tccagtcgat gtccttccgg cgattgagga
      961 tcgcatcggc cggttcaatc cagtttttcc ccgtctggtc tgagacggta tgatagcgct
     1021 cattggtaat ccatccggta tcgcgcatga acttgaacac cggatcttcc gtgctcttga
     1081 aggcccaggt accgcacggg atttcggggg atcgctgctc actgatccca ggggcagtgg
     1141 atgcgcaggg gtacgggtct ttctggacca gttccccata ttcgacggcg ttcgccctgt
     1201 ccgctcccca aagcaggagc agcactgtga gtaagaccca ccataacgat tgcttcgaca
     1261 tattctcctc ttaattccgg cggttgttgt gaaagtgtgt tgtcaacctg gcgagagcat
     1321 aattagacga cccgcagggc atgcactgtc ccggtctccc gggtgtcgta tcctcctaag
     1381 gcttggagct cgtttcgaaa cggtcccctc acaatggtgt cgaacaagtg ggctaacgtc
     1441 ggatgagatt gtaagtaggg cttggggacg accagatcgt agcgggcggc ttggagtgga
     1501 acgaagtcga gtccaaacag ttgcgctgcc gaccgaattg cgattccaac atcggcctgg
     1561 cggccatcta tggatcgagc cacttcaaca tgagaggaca caatccgatc gtatccttga
     1621 atctgtgtcg actctacccc ggatgcccgc aatcgttgat cgagcagcag tctcgctccg
     1681 gagccttctt cccgattgac cagtgtgatt gccgtctgag caagatcggc ggcagtgcga
     1741 atggacaacg gattgccctt acgaactaaa aagccctctt cccacgtggc aaaggtgacg
     1801 acctcatagg ccgatccttt gaggtgtcgc cgaaggaacg gtaggttcga ttcgccggta
     1861 atgggatcaa agagatgcaa tccggccaca tggacttcgc cacgctgcaa ggcctgcaat
     1921 gccgccatac ttcccatcgg ccaaccgacg accgtcgtct gatctttgtg tcgccgtaaa
     1981 tattcgccgg ccaggaaaat tgccggatcg catcctgcca cggagatttc ttgttggatt
     2041 gcctgacgat ctcgcgagag tgttacacgc acactcgtac cagagggcgt gtgtcgcgtg
     2101 tcggtgatat atccgtcggc aggaactgca aaggaaagtt gctctcccaa atcggcgaca
     2161 ggtcgcacaa ccgtccgctt tccaacggtc gccactttga cacggatcaa ttgagtctgt
     2221 gtctccgctt gaaggaaccg gccgataagg gtgccttcaa tgatctcttc tgcctgagca
     2281 agactgaaca gatcttcgac gcggcaagcc agaaccgagg ccaatcggag ggcgactgct
     2341 gtcgtcggca agtactgatt cgactcaatg gaagatacgg cctgcctcgt aatacctgct
     2401 cgattggcga gttcaccctg agaaagaccc tgcttcgtgc ggacagattt caggtgattg
     2461 gaaaattgaa cggtgtttcc atgatgtttg atccgcgtcc gcatggggtc actgtgtagc
     2521 aagtatacat tgcatctgtc aataatgaat tacatcactt tgcaggtttc tatgagctat
     2581 tgatttgtac taagcagaat aagcgataaa taattctatt attaatgttg ttgacaaaga
     2641 aactttagtt ttattataag aatgctacaa attctgatcg ataatataaa gattgattaa
     2701 gccgttgcga aagaagagtt aaaagcgtgt ccagagcaga tcaagacatc acgaatccat
     2761 actcagctcg aactgtcgaa caagcaatcc aactggcctt gaaagggatt cggtttgggt
     2821 ccgttgagat catcgttcac gactccaagg tggtgcagat cgaacgaaaa gagaagacac
     2881 gcttttttga tgacacacgt tctcgaccaa ttcgatgagc cagcgacagc ggtttgaaag
     2941 tatcgtggtt ggaagtattc aaatgatgac ctgaattgtt caggcgactg gaagggcgag
     3001 agttttttaa cttattcaac taagaactga ccggactacc ggaagttccc atgtcatgga
     3061 ggaatgcgac atgagaattt ctggttcaag tgtcttgttt gcgatcatga tgatctccct
     3121 tacatacatt tcgtctgctt gggctgtaga ggagggccaa ttcgtcaaga aggatggcaa
     3181 gtgggaattc tattcagggg aagaccccgg cttgaagtat ctctacctga aagggttgat
     3241 cacggaagaa gagtacagta agggcctcaa ggtcattgaa accaaagagc ggctctcaaa
     3301 gccgaatttc acgatcgatg tgaacaatgg attgaacttc cgtgtcgggg aaaagtttct
     3361 gcttaaactc cgtctcctta cgcaagtccg gtatagctac agcaggtaca acgaagcctg
     3421 ggggacgatt ggagattcca ggaatccaga aattctcggt ggccaagtcg aatatcgcgc
     3481 gttccggcat cagagcgatt cgagtacatt ttcggccccc cgtgttcaac tccaattctt
     3541 gggtcatgcc ttcgaccctg attttcgtta caacgtttcc gtggcgttga atcagacgga
     3601 atgggaccaa gagggaggca gcggacgagc caatctgctc gatgcataca tcgcttcgtg
     3661 gcacattccc tgggctaccg ttcaggcggg ccaacagcgg gtatggttca atcgtgctct
     3721 gatcagttcc aatgccacta cttcttttgc agaccccatg gttgtgcaaa gtgtgtttgc
     3781 atccaacctg cagggtagcc gcgacgtggg agtcaccata ttgagcgatg aggagaaata
     3841 caggttcaac tatgccttcg gcatttgggg aggagtcggc gcaaatctga cgagagaggg
     3901 tacggcagtc agtcagaatg tgttgcctcc caccggcggg ggaggtccca atgctggaac
     3961 cacaagaaca tacaactatg atacccgttt cctgacgggt gaaatgatgt acacgatcag
     4021 attactctat aaaattgcgg gtaatcccgg gtacggtcag ggagatattc tcaattcacg
     4081 tactccgcag gcagccatcg cgttcggcta tgcctacgat ccggctcaaa actatctgag
     4141 ctcgattcga tccgacatcg tcgaccgggc attccgtcag cgagtggtca aggcctataa
     4201 cggcagattg ctcggaggcg gtatctatga ctttcacacg tatgaactgg atttcatcgc
     4261 gaaataccgc ggctggtcga ttcaagccga aggatttttc cgcgaccaaa ttgtacggaa
     4321 tgcggatagc caaacccgac cgttcgatct gggaacaatc ccgacgagtc cgctcgccgt
     4381 cggtcccccg gtgtcgctgg gtgatgcttg gggctggtat gtgcaagtgg ggaagtacgt
     4441 gattccgcgc aaactcgaag ttgcggtccg atacggcatc cttgatcctt cgaccagaca
     4501 aaaacaggac ttgaccaagg aactgggcgt tgcggtcagc tacagcttcg acggcaccta
     4561 caataatcga ctcattatcg attactcgaa tatcacgatg ggaagcggcg ggcgggctcc
     4621 ggatcggttc cccttcgaga atttgccagg gttcggtcgg gacctcgttg aaaaccggtt
     4681 caacgtgcag taccagtttt atttctaatc gtacatggaa cggtagagat acaaggaggc
     4741 tcccatgata ttcacgcata cttttagccg cataattgtc ttcatcggta ttaccgtttt
     4801 cacggggccg gtggttctct ccgcccatgc ccaatctgaa gttctcaccg tcgcggcggc
     4861 gagcaactta aaagacgtgt tccgaaagat attgccgctc tttgaagcgc aaaaccacga
     4921 tatttccgtg cgggtcattt atgctcagcc taaaacactt gtcaaacaaa tcgaggaagg
     4981 tgcgccggtt gacgtgctga tccctgcgca attcgaggat atcgatcaac tggaaaagaa
     5041 agggctgatc cttcaagaca ctaaacaagt ctatgcccat acggcactcg ttttaatcac
     5101 caatgccgct ttccctgcac cggtcggctc gattcaggat cttgagacca ccccggtcag
     5161 gcgcattg
//
LOCUS       DATNDD010000068         3066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1053675, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000068 DATNDD010000000
VERSION     DATNDD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053675"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VN647_01480"
     CDS             <1..236
                     /locus_tag="VN647_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWG95726.1"
                     /translation="VTRGGAADRAGLKGARQTMIGKIQLGDVIISVAGKAVATIDDLM
                     DVMEAHKVGDQVSVEIVRENRRETVPVTLQAVN"
     gene            309..2045
                     /locus_tag="VN647_01485"
     CDS             309..2045
                     /locus_tag="VN647_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWG95727.1"
                     /translation="MSDRRVSEQDGPNELSEEASLKTGPDPLELIEQCLAEFPEGDAR
                     QKLLYKLRHAVTLSQAAHQQRELEFKKVSEIVAKLTAPANRIGTLLDVPGEGLARIVV
                     GGAEYYANVDPRVPETDLKIGTQILVNEAYAVIKTLGYDRNGPIVKLAEAMPDGRLRC
                     EQEMGRQSLVLQRSTDLIGVDLDPGDEIRIDPSHRVAIEKLVDRKASRHVLDETPKVT
                     WEQIGGQNEAINAIRKAIEYPLLHAETFERFKFSQPKGFLLYGPPGCGKTLIGQAAAG
                     SLAKLVGESKQEHMPDRGKRPPVTSGAFLHVKGPEILNMWLGESERMVRDLFEQARAR
                     RRDGALPFIFIDEAESILGTRRAMRSFNISNTLVPMFCSEMDGIESLRDVVIILASNR
                     PDLIDPAVLRPGRIDRKIKVSRPNKDAAAEILKVYLTADLPLDPVLVKERGCEQSQAV
                     DSLVADIIESIFRRTEDNRLLSIRLRTGQNKVLYRSDLMSGAILASIVQRAKEKAIDR
                     TVQSGQQSGLLAKDLLDSVSEEFREGDMLPPDDAAEEWLKLLDHHPEQVVGISSFRRG
                     RLTEERLVNQII"
     gene            2144..>3066
                     /locus_tag="VN647_01490"
     CDS             2144..>3066
                     /locus_tag="VN647_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWG95728.1"
                     /translation="MRLFGIETEYGITRDDVPEMDPVVESMELVRAYLEASFERRWDY
                     AGEDPHEDARGFRVSGLQQDREEDEFAKVDAHRPFSFHEMKSDLVLPNGARFYNDHTH
                     PEYSTPECRTIRDFLAQDRAGERILLRAAQRRNRTLGGDHVRLYKNNTDFHGHSYGCH
                     DNYLVSRAVPFPALVAGLVPFLVSRQVIVGAGKVGTEAQESGYVPGRYQLSQRADFME
                     AELGVDTMHNRPILNTRDEPHADRRKYRRLHLIIGDANMCEYATALKVGTTRLVLDLI
                     ERGAMPHIELEQPVVAVKQLSRDPDLKAVVRL"
BASE COUNT          710 a          751 c          951 g          654 t
ORIGIN      
        1 aggtgacgcg cgggggtgcc gcagatcgcg cgggcctcaa gggggcgcgt caaaccatga
       61 tcgggaagat tcaactcggc gacgtcatca tctcggtcgc cggcaaggcg gtggccacca
      121 ttgatgatct gatggatgtg atggaagcgc acaaagtcgg tgatcaagtg agcgtggaga
      181 ttgtgcgaga aaatcggcgt gaaacggtgc cggtgactct tcaggccgtc aattagtatg
      241 tagtgtgaag gcattgcaag acatgtgtgt gttaggatca tttggtcaag cgtcatgaag
      301 gaccacctat gagtgatcgc agagtttctg aacaggatgg acccaacgag ctgagcgaag
      361 aggcgtcgct gaagaccggg cccgatccgc tcgaattgat agagcaatgt ctggccgaat
      421 ttcccgaggg ggatgctcga cagaagctgc tgtataagct gcgccatgcg gtgacattgt
      481 ctcaggccgc ccatcaacaa cgcgaactgg aattcaagaa agtaagcgag atcgtcgcca
      541 agctgacggc accggccaat cgtatcggga ccttacttga tgttcccggc gagggactcg
      601 cccgcatcgt cgtcggtgga gccgagtatt atgccaacgt ggatccccgc gtgccagaaa
      661 cggatcttaa gattggaaca cagattctgg tcaatgaagc gtatgcggtg atcaaaaccc
      721 tgggttacga caggaacggt cctatcgtaa aactcgctga ggctatgccc gacggccgat
      781 tgcggtgtga acaggagatg gggcggcaat cattggtctt gcaaagatcg accgatctta
      841 tcggcgtaga cctcgacccg ggggatgaaa ttcgtatcga tcccagccat cgcgtggcga
      901 ttgaaaaact cgtggatcgc aaagcaagcc ggcacgtcct cgatgaaacg ccgaaggtca
      961 cttgggaaca gatcggcgga cagaacgagg cgatcaacgc cattcgaaag gcgatcgagt
     1021 atccgttgtt gcatgcagag acgttcgaac ggttcaagtt ttcccaaccg aagggctttc
     1081 ttctctatgg cccacccgga tgcggcaaga cgctcatcgg ccaagcggcc gctggaagct
     1141 tggccaagct ggtcggcgaa tccaaacaag agcacatgcc tgatcgaggc aagcgtccgc
     1201 cggtgacaag cggagcattc ctgcatgtga aagggcctga aattctcaac atgtggttgg
     1261 gtgaatcgga acggatggtt cgcgatctct tcgaacaggc acgtgcgaga cggagggacg
     1321 gcgcgttgcc gtttatcttc atcgacgaag cggagtcgat tttggggacg cgacgcgcta
     1381 tgcggtcctt caatatctcg aacacgctcg tgccgatgtt ttgcagcgaa atggatggga
     1441 tcgaatcgct acgtgacgtc gttattatat tggcttccaa tcgtcctgac ttgatcgatc
     1501 cggcggtgct gcgcccaggc cgcatcgatc ggaagatcaa agtcagtcgg ccgaacaagg
     1561 acgctgcagc cgagattctg aaggtgtatt tgactgcaga tctgccgttg gatccggtgc
     1621 tggtgaaaga gcgaggttgc gaacagagtc aagcagtgga ctcgctcgtc gcagacatca
     1681 ttgagtcgat tttccgtcga acggaggata atcggcttct gtcgatccgg ctcaggaccg
     1741 ggcaaaacaa ggtgctgtat cgtagcgatt tgatgagcgg agccatcttg gcctcgatcg
     1801 ttcaacgtgc caaggaaaag gcgatcgatc gtacggttca gtcggggcag cagtccggat
     1861 tgctggccaa agatctcctg gattccgtct cagaggaatt tcgagaggga gacatgttgc
     1921 cgccggacga tgctgcggag gagtggttga agctgctgga tcatcacccg gaacaggttg
     1981 ttgggatttc ctcgttccgg cgggggcgcc tgacggaaga acgcctcgtg aatcagatca
     2041 tctgaagcag tgaaatgcga gaagtaacag gtgaaaagtt gagagcccgg atgttgcccg
     2101 gttcacgttt tacatttcac atctaacgta ccattaacac gccatgcgcc tttttggcat
     2161 cgaaaccgag tacgggatta cgagggatga tgtaccggag atggatccgg tcgtggaatc
     2221 gatggaattg gtgcgggcct atctggaagc ttcttttgaa cgccgttggg attatgccgg
     2281 tgaggatccg catgaggatg ctcgaggctt tcgcgtttcc ggcctccaac aagataggga
     2341 agaagatgag tttgcgaagg tggatgccca tcgccccttt tcgttccatg aaatgaagag
     2401 cgatttggta ttgccgaacg gggcgcgatt ctacaacgac catacgcatc cggagtattc
     2461 gacgccggag tgccggacga tcagagattt tcttgcccag gatcgcgcgg gagaacgtat
     2521 tcttctgcga gctgcgcagc gacgcaatcg aacgctcgga ggagatcacg tcagactgta
     2581 caagaacaat acggatttcc acgggcacag ttatggatgt cacgacaact atcttgtctc
     2641 gcgggccgtt ccctttccgg ctctcgttgc cggcttggtg ccgttcttgg tgagccggca
     2701 agtgatcgtg ggtgcgggaa aggtcgggac tgaagcacag gagtccggtt atgttccagg
     2761 acggtatcag ctctcgcagc gcgctgattt tatggaagcg gagctgggtg tcgatacgat
     2821 gcataatcga ccgattctta acacaaggga cgagccacat gccgaccgcc gaaaatatcg
     2881 ccgactccat ctcatcatcg gcgatgccaa catgtgcgag tatgcaacgg cgctgaaggt
     2941 cgggacgacc agactcgttc ttgatttaat cgagcgaggg gctatgcctc acatcgagct
     3001 ggagcagccg gtggttgcgg tgaaacagtt gtcgcgcgat ccggacctga aagcagtcgt
     3061 tcgtct
//
LOCUS       DATNDD010000069         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1247677, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000069 DATNDD010000000
VERSION     DATNDD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247677"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1396)
                     /locus_tag="VN647_01495"
     CDS             complement(104..1396)
                     /locus_tag="VN647_01495"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HWG95729.1"
                     /translation="MIAQDSLGAVRYRLLEDPTQPGMTMKKQIGIDELQQGMVIEQVD
                     RSWLDTPFFRHKMTITSSQQIAQLKACGVKTLVVSIEAEEVEQGTVFEPDITDEPIRL
                     AEDETASVPQVVPFEDELPVARQVYQAAKTIVQNAMHDTRLGRAINVDAVDQIVSEMT
                     DSVLRNPDALSSLSRLKRFDEYTFYHSVNTALLAMSLGRKLGFDRSTLHLAGVGTLLH
                     DIGKMKIPLEILNKPGRFEAHEMEIMKQHVLRGVEVLAGTTGLGDSYVQPALEHHERV
                     NGAGYPHRRAKQDISQFGLITAVVDIYDAMTSDRCYHKGRPAHEILQLLYRLSLEGHL
                     DSTLVQRFIQVVGVYPVGSIVELNTGETGIVKQVNHHAPLAPVVLLVKSAGNTLLSSP
                     HEQDLSAQIERPHRSVATILDPHQAGIDPTLYLDKKAT"
     gene            complement(1505..1912)
                     /locus_tag="VN647_01500"
     CDS             complement(1505..1912)
                     /locus_tag="VN647_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95730.1"
                     /translation="MNLVRLAIAVSLVVLQYACATTTETLSAPPGSNATAALHNSEGI
                     VHYEMGHWSIAKDHFAAAVAADSNLAEAHFNLGLALNKLNLQSEATAHFKKAAELAPA
                     DSSIAESSAYRSRTAPPSPSSYGADGYGGMGGY"
     gene            complement(2031..>3406)
                     /locus_tag="VN647_01505"
     CDS             complement(2031..>3406)
                     /locus_tag="VN647_01505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWG95731.1"
                     /translation="VRMEATSGSTPMNLQTFILISKDGELGHLLRAASPQAGIHTTNT
                     VSEGVALWSEISPDLIIGEGPLSILAPLLEHARQLPVCPPLLVIGDSHSVKDAVEIMR
                     AGATDYLPRPICIEDLQAAVTRALGHPTSTPPSSPQDPFTSIISVSAQMNLMKRLAKE
                     VAITDATVLITGESGTGKELFAEAIHRCSLRANGPLITLNCAGIPENLLEAELFGYES
                     GAFTDAKRSKPGRFQMAEGGTIFLDEIGEMSPTAQAKLLRVLENHTIDPLGDTRSRKV
                     NIRIIAATNEDLLAHIKAGRFRLDLYYRLNVYQLRIPPLRERLEDIEPILLIFLERAK
                     QERGCRIRTIAPAALAVLQSHDWPGNVRELHNVVEWLTITCKAEIIQPTHLPASVKTA
                     PPTNCLSQNSNPSLLAFGLSFQDMEKQMLEEALRKSSGNVSEASRLLKMTRNTLRYRM
                     AKYHLHE"
BASE COUNT          721 a          868 c          991 g          826 t
ORIGIN      
        1 tgcgtagcga tggtgcaatt ctgcctgatc gcgaacgatt taggctatcc tgctggccgc
       61 ggcccaagag tgtgaggtga ggtgacggtg ctgatcctat ttattatgtt gccttcttgt
      121 cgaggtagag agtcgggtcg attccggctt ggtgcggatc gagaatggtg gcgacgcttc
      181 gatgcggcct ctctatctgc gcggaaagat cttgctcgtg cggacttgag agcaacgtat
      241 ttcccgcgct ctttacgaga agcaccactg gcgccaatgg cgcatggtgg ttcacctgtt
      301 tgacgatgcc ggtttcaccg gtattcaact ccacgattga tccgaccgga taaaccccga
      361 cgacctggat aaaccgttgg actaacgtcg agtcgagatg gccttcgagc gagaggcgat
      421 agagaagctg gaggatttca tgggcgggtc gtcccttgtg gtagcatcga tcgctcgtca
      481 tggcgtcgta gatgtcgacg acagcggtga tcaagccgaa ctgactgatg tcttgcttcg
      541 cgcgccgatg cggatatccg gctccattca cccgttcatg gtgttcaagg gccggctgga
      601 cgtatgaatc tcccaaaccc gtcgtgccgg ccaagacctc gactcccctg agcacgtgct
      661 gtttcatgat ctccatttca tgagcctcga agcgaccagg cttgtttagt atttcgagcg
      721 ggatcttcat cttgccgata tcatgcaaca gcgttccgac tccggctaaa tgcaatgtcg
      781 accggtcgaa gccgagtttt cgccctaagg acattgccag tagtgcggtg ttgacggaat
      841 gataaaaggt gtattcgtcg aatcgcttga gtctcgatag actggacaaa gcatctggat
      901 tccttaagac gctgtcggtc atttcagaca cgatctgatc gacggcatcg acattgatgg
      961 cccgtccgag ccgcgtgtca tgcatcgcgt tctgcacgat cgtcttggcg gcctgataga
     1021 cctgcctcgc aacaggcaac tcatcttcga aggggactac ctgcggcacc gatgccgttt
     1081 cgtcctctgc caatcttata ggctcatccg tgatgtccgg ctcgaagact gtgccttgtt
     1141 cgacttcttc tgcctcaata ctcacgacca gggtcttcac tccacaggct tttagctgtg
     1201 cgatctgttg agacgaggtg atcgtcatct tgtgtcgaaa aaacggcgtg tccagccagg
     1261 accgatcgac ctgttcgatc accatgccct gctgcaattc gtcgatgccg atctgttttt
     1321 tcatagtcat accaggctga gtcggatcct ccagtaagcg atatcggact gctcccaagg
     1381 aatcttgagc aatcattgca tcaccgccga tacgtagatc cgaattctgc agttgagcag
     1441 atggcagggg agaagatagc ttgagggtga atcggatgac cgccgatagt cacctagcca
     1501 ggaactaata tcctcccatt cccccatagc catctgcgcc gtaactggat ggtgaaggcg
     1561 gagcggtgcg gcttcgatag gcgcttgact cggcgattga gctgtcggcg ggcgccagtt
     1621 cagctgcttt tttaaaatgg gccgtagctt ccgactgaag attcagttta ttgagcgcga
     1681 ggccaagatt gaagtgggcc tcggcgagat tcgaatcggc cgcgacagcg gccgcgaaat
     1741 gatctttggc gatcgaccaa tggcccatct catagtggac aatcccttcc gagttgtgta
     1801 gggcggcggt ggcattgctg cctggcggtg cagagagtgt ttcagtcgtg gtggcacaag
     1861 catattgcag gactacaagg cttactgcta tggccagtcg gacgagattc atggttcacc
     1921 tccagagtaa gaatccatgc ttgcgatggt cgagaagacc agcggacgga gactgtgaat
     1981 ccgaagcaaa gggaagcggc ctgcatgcca aataactaga cacggttcct ctattcgtgg
     2041 aggtggtact ttgccatacg gtagcgcagg gtgttcctgg tcattttgag aagtcgactg
     2101 gcttccgaga cattccctga cgactttcgc aacgcttctt caagcatctg tttttccatg
     2161 tcctgaaacg acagcccaaa ggccagcaac gacggattgg aattctgact cagacaattg
     2221 gtcgggggag cggtcttcac tgatgccggt aaatgagtag gctgaataat ctctgctttg
     2281 cacgtgatgg tcagccattc gacgacattg tggagctcgc gtacatttcc aggccaatcg
     2341 tggctttgaa ggaccgcaag agctgccggg gcgatagtcc taatacggca tccgcgttcc
     2401 tgtttggccc gttccaaaaa aatcaagaga atcggctcga tatcttcaag tcgttcacgc
     2461 aagggtggaa tgcggagctg gtagacattc aaccgatagt acagatcgag acggaaccga
     2521 ccggccttga tgtgggcaag cagatcctca ttcgtggcgg caatgatgcg gatgttgacc
     2581 ttacggctgc gggtatctcc caagggatcg atggtgtggt tttctagtac gcgtaggagt
     2641 ttggcttggg cggtcggact catttctccg atttcatcga gaaagatcgt tcctccctcg
     2701 gccatttgga atcggcctgg ttttgaccgc ttggcgtcgg tgaaggcacc cgattcataa
     2761 ccgaataact cagcctccaa gagattttct ggaatccccg cgcaattgag cgtgatcagc
     2821 gggccgttcg ctcgtaggct gcatcggtgg atggcctctg caaacaattc ctttccggtt
     2881 ccgctttcac cggtgatgag gacggttgcg tctgttatgg ccacctcttt ggccagtcgt
     2941 ttcatcagat tcatttgcgc tgagacgctg atgatggagg taaatggatc ctgtggggaa
     3001 gagggtggag tcgaggtagg atgtccgagt gctcgagtga cggcagcttg cagatcctcg
     3061 atgcagatcg gtctgggcag ataatcggtg gcgccggccc tcatgatctc gaccgcgtct
     3121 ttgacggaat ggctgtcccc aatcacaagc aagggagggc agacgggtag ttgtcgggca
     3181 tgttcaagca ggggagccag aatggagagg gggccttcgc caatgatcag atccggggaa
     3241 atttccgacc atagagcaac tccttccgat accgtgttcg tggtatgaat accggcttga
     3301 ggtgaggcgg ctcggaggag atgcccaagt tcgccgtctt tcgagatgag gataaacgtc
     3361 tgaaggttca tcggagtgct cccgcttgta gcctccatcc gaacca
//
LOCUS       DATNDD010000070         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_27324, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000070 DATNDD010000000
VERSION     DATNDD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27324"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VN647_01510"
     CDS             complement(<1..304)
                     /locus_tag="VN647_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HWG95732.1"
                     /translation="MQLSDFDFPFDSLLIASQPILPRDRARLLIVDRATRQLVHRQVV
                     DLPELLQSGDLLVVNDTRVRAARVPGIKRPTGTPVEVLFVKDLGDSRWEIMVKGSLR"
     gene            complement(304..1527)
                     /locus_tag="VN647_01515"
     CDS             complement(304..1527)
                     /locus_tag="VN647_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HWG95733.1"
                     /translation="MIAAGLLAGTAGLGLSLGSVMIPEVHAAQSIRVLLAPDVQQLEV
                     HTDQTIWMTDEHDQASSYRSVLRIKVRGHALILNGKPVVSERLTLRAGNHDLKLRLNQ
                     NGNRTALHAGDEKGVLQVSGLIQLVRRGKGLLLINHVDLEEYVKGVVPAEVNPAWHLE
                     MLKVQAVATRTYALYQHMLSSTRDYDVVSGIQDQVYRGRQGIDARVVEAVESTRGLVV
                     TYQGAPIYAAFSSTAAGITEDAMIVWSKDLPYLKGVECPFDIESPHYQWKASLKIDTL
                     EKNLRQQGFTVGTISAIMPLAYSRAGRVATLRIVHSSGELILRGEDLRKAVGYTVVPS
                     TQFVVESVGQDVVISGYGAGHAVGLCQWGAKELAELGYSFSSILRYYYPGTELQDASL
                     IQAPSVPSPLSAPAS"
     gene            complement(1524..1829)
                     /locus_tag="VN647_01520"
     CDS             complement(1524..1829)
                     /locus_tag="VN647_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWG95734.1"
                     /translation="MCCLTGLLGHVTHRTKVIPEWTTFGKVLIGIGIGIAALGFLLVV
                     VDRIPGLGNAFAWLGKLPGDISIKRDNFSFYFPIATSIVLSVLLSLLFYLVGWFFRR"
     gene            complement(1833..>2135)
                     /locus_tag="VN647_01525"
     CDS             complement(1833..>2135)
                     /locus_tag="VN647_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HWG95735.1"
                     /translation="RVPVLRCHSEAINVELERPLKANEARAALAAMPGVIVYDDPVKK
                     LYPMPLDATGKDEVYIGRVREDESIANGLNLWVVSDNLRKGAALNAIQIAECLVNV"
BASE COUNT          518 a          642 c          542 g          433 t
ORIGIN      
        1 cccgcaatga gcccttgacc atgatctccc atcggctgtc accgagatcc ttcacgaaca
       61 acacttcgac cggtgttcct gtgggtcttt tgattccggg aacccgcgca gcccggaccc
      121 tggtgtcatt cacgacgaga aggtcgcctg attgaagtaa ttcgggaaga tctacgacct
      181 gacgatggac aagttgtcgc gtcgcacgat ccacgatcag cagccgcgca cgatcccgag
      241 gaagtatcgg ctgggacgca ataaggagag aatcgaacgg aaagtcaaag tcggacagtt
      301 gcatcaggat gcaggggccg agagggggga aggcactgaa ggcgcctgga tcaacgacgc
      361 atcctgaagt tccgttcccg gatagtagta gcgaagaata ctcgaaaagg aataacccag
      421 ctcagccaac tctttcgcac cccactggca aagaccgacc gcatggccgg cgccatagcc
      481 ggagatgacg acgtcctggc cgaccgactc aacgacaaat tgcgtactgg gtaccaccgt
      541 ataccccacc gctttacgca gatcttcgcc tcgtagtatc aactccccgc tcgagtgcac
      601 aatccgcagc gttgccactc gtccggcgcg gctataggca agcggcatga ttgccgaaat
      661 tgttcccacc gtaaatcctt gttggcgcaa atttttctcc aaggtgtcga tcttcaagga
      721 agccttccac tgatagtgcg gagattcaat gtcgaacggg cattccacgc ccttcagata
      781 cgggagatct ttcgaccata caatcatggc atcctcggta atcccggcgg cggtcgaaga
      841 gaacgcggca tagatcggag cgccttgata ggttacaacc agtcccctcg tggattccac
      901 cgcctccacc acccgcgcgt cgatgccttg ccggccgcga tacacctgat cctgaatccc
      961 cgagaccaca tcatagtccc gagtggagct aagcatgtgc tgatacaatg cataggttcg
     1021 agtagcgact gcctgaacct tcagcatctc caggtgccag gcgggattca cttccgcagg
     1081 caccacaccc ttgacatact cttccaaatc gacgtgattg atgagaagca gtccctttcc
     1141 tcgccgaaca agctggatca acccactgac ttggagtacg cctttctcat cacccgcatg
     1201 gagcgcagtc ctgttaccat tctggttcag ccgaagtttg agatcgtgat ttccagcccg
     1261 taacgtcagt cgttcactca ccaccggttt gccattgagg atcaacgcat ggccacgcac
     1321 cttgatccgc aggacagacc gataggacga cgcctgatca tgttcgtcag tcatccaaat
     1381 ggtctgatca gtgtgcactt ccaactgctg gacatccggc gccaacagca cgcgaatcga
     1441 ttgcgcagca tggacctccg gaatcatgac cgagccgagg gagaggccca gtccggcagt
     1501 tccagccaaa agccctgctg ctatcatcgg cgaaagaacc atcctacaag atagaatagc
     1561 agactcagaa ggacgctgag gacaatactg gtcgcgatgg ggaaatagaa gctgaagtta
     1621 tcacgtttga ttgaaatgtc acccggaagc ttgccgagcc aagcgaaggc gttccctaac
     1681 cctggaatcc ggtccacgac gaccaataga aacccgagcg cagcaatacc gatgcctatt
     1741 ccgatcagga ccttgccgaa cgttgtccat tccggtatca ctttcgtcct atgggtgacg
     1801 tgcccaagca atcccgtgag acagcacatc tactagacgt tcactagaca ttctgcgatt
     1861 tgaatggcat tcagcgccgc tcctttgcga agattatcgg agaccaccca aagattgagg
     1921 ccgttggcaa ttgattcatc ctcccgcaca cggccgatgt agacctcatc ttttccggtc
     1981 gcatcgagcg gcatcgggta cagcttcttc accggatcgt cgtagacaat cacgccgggc
     2041 atggctgcca gcgcagcacg cgcctcgttc gccttcagag gacgctctaa ttcaacgtta
     2101 attgcttcag aatgacagcg caacacgggc acacg
//
LOCUS       DATNDD010000071         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4085787, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000071 DATNDD010000000
VERSION     DATNDD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4085787"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VN647_01530"
     CDS             complement(<1..913)
                     /locus_tag="VN647_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWG95736.1"
                     /translation="MILRTLLDRYIFTELLSPFGLSLGALCFVMLSRELLRLVELLVS
                     KGVGFWAVIKVIAMLLPSGLVLTLPIAGLIASVTAFGRLSYDKELVAMRATGLSLFRL
                     SQPVLLFSVCVFTLTLVLSQWGQPWSSLNLKKVALNLLKDQLVLALERGTFNEPIPRM
                     MIYVPEGDSGRPAEGIFISDERLPEEPRVIVAEQYRVFMDAAHAQVALRLVNGVIHSR
                     PGQVDQYRQIAFASYDLKIDLNLSSYSATEERPSYEQIMARLAESGGKDAGSLRRLME
                     YYKDLAFPTASLVFCLLGVPVGIVSKRS"
     gene            1229..1447
                     /locus_tag="VN647_01535"
     CDS             1229..1447
                     /locus_tag="VN647_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWG95737.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDSGEDVFVHYSALQGEGFKTLKE
                     GENVEFDIVQGAKGPQAANVLKSAIVAS"
     gene            1600..>2111
                     /locus_tag="VN647_01540"
     CDS             1600..>2111
                     /locus_tag="VN647_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95738.1"
                     /translation="MPPDRSRVLQQAQLLASRGQYEAAIAEWKKLATETPADGTIHNT
                     IGDLQLKRNAPGEAASAFLQAASAFRTEGATLKAIAAFKKVLKCDPSRYEVYRHLGDL
                     NIERGLISSAVQDYLTLGKYYLKERRGKDALEIYRKIVLQDPSNLNAQQRVAELCVQE
                     NQQDEATKVYL"
BASE COUNT          546 a          556 c          566 g          443 t
ORIGIN      
        1 cggaccgctt cgagacgatt ccgaccggta cgccgagcag acagaatacg agggaagcgg
       61 tcggaaaggc caggtcttta tagtattcca tcagacgacg aagcgagccg gcatccttac
      121 caccggactc ggccagccgt gccatgatct gttcatagga cgggcgctct tcggtggccg
      181 aatagctgct gaggttcaga tcaatcttga ggtcgtaact ggcgaacgcg atctgacgat
      241 attggtcgac ctgacccggt cggctatgaa tgactccgtt caccagccga agcgcgacct
      301 gcgcatgggc ggcatccata aagacgcggt actgttccgc gacgatgacg cgaggttctt
      361 cggggagacg ttcatccgag atgaagatgc cctcggccgg acggcccgag tctccttctg
      421 gaacatagat catcatgcgt ggaatcggct cattgaaggt accccgctcc aaagccagaa
      481 caagctgatc cttgagcaga ttgagagcca ccttcttgag attcaatgag ctccatggtt
      541 gcccccattg agacaagacc aatgtcagcg taaagacaca caccgagaag aggagtaccg
      601 gctgcgagag gcgaaacagg ctgagtccgg tcgcgcgcat ggcgaccagt tccttgtcat
      661 aagacaaccg gccgaatgcc gtgaccgacg cgatcaggcc ggcgatcggg agcgtgagta
      721 cgagaccgga cgggagcaac atggcaatca ctttgataac tgcccagaaa ccgaccccct
      781 tggataccag cagttcgaca agacgcaaga gttccctgct caacatgaca aagcagagcg
      841 cgccgaggct gagaccaaac ggagagagaa gttccgtgaa aatgtagcga tcgagcagcg
      901 ttcgtaagat cacagaatgt ctggcaatgt ctgaattgga gaaatttcga acactatagg
      961 ggagtccttc gcacctgtaa acacaggaga aaatcatcgc cttaaagatt cgcttgacag
     1021 agtatgccac ctatgttaca tgcagcgcga ctttccactc aaatgatggg ttccgtgagc
     1081 gatgcatcat tccttcaggt gcccgtccct tctaccagcc ggattcttgt ccaatctatt
     1141 ttcaattcta tttttaattt cgatacaggg gcgattgagt ggggccgcta cgtaaccagt
     1201 cgtagttgtt gacaaaagga gggcttctgt gaagacaaaa ggaacggtca agtggttcaa
     1261 cgatcgaaag ggatttggat tcatccgact ggatagcgga gaagacgtct tcgtccatta
     1321 ctccgcgtta caaggagaag gtttcaagac tctcaaggag ggggaaaacg tcgagtttga
     1381 tatcgtgcag ggagccaagg gccctcaggc ggccaatgtg ctcaagtcgg cgatcgtcgc
     1441 atcctaagtc gtcatatctt ccgtttggct cggtagaaga accgatcctc cgacaagagg
     1501 gtcggtcctt ttaccgagtg tttcaccgtc tgatttctcc attttctaga caaaaacctt
     1561 tgacactgtt agcgtttggc tgttcgtgga ggtatccttg tgcccccgga tcgaagtcga
     1621 gttcttcaac aagctcagct cctggcttcc cgcggacagt acgaagcggc gatagccgaa
     1681 tggaagaagt tagccacaga aacccctgcc gacggcacca ttcataatac aatcggtgat
     1741 ttgcaattga agcgaaacgc tccaggggaa gccgcctctg cattcttgca ggcagcctca
     1801 gccttccgaa ccgaaggcgc cacccttaag gccattgccg cctttaaaaa agtccttaaa
     1861 tgcgatcctt ctcgatacga agtctatcgc catcttggag atctgaacat agaacgtgga
     1921 ctcatcagca gtgcggtcca ggactacctc accctgggga agtactactt aaaagagcgt
     1981 cgaggaaaag acgcgcttga aatctacagg aagatcgtcc tccaggatcc gtcgaatctc
     2041 aacgcccaac agcgagtcgc cgaactctgt gtccaggaaa accagcagga tgaagcaacc
     2101 aaagtgtatc t
//
LOCUS       DATNDD010000072         9361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3267150, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000072 DATNDD010000000
VERSION     DATNDD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267150"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1881)
                     /locus_tag="VN647_01545"
     CDS             complement(235..1881)
                     /locus_tag="VN647_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWG95739.1"
                     /translation="MRAVVGLLTFCGPAESAFELPEGEKITNPIVIGRGIPQKEAYEL
                     FDPKIGRNFDLKNLWMRADLRVRPEFRNNVCFGGGIGASGECNGPGIAPNNLTGNAND
                     QFVQQMTRLGIGYDLSPDVNFYLEFIDSRTWGGNGSPVGAGGAGNNGDPSIHTCGSTP
                     NPGGRPVCTLGVRAGYMLIRNFAGIQGLGLKAGRQYLVFGDQSLFGHFDWANTGFSFD
                     GVMVQYSNSIMDTHAGWFRTAETDLFQGAAVGSGQANIVGTGQTRNATGDADLFIFYN
                     QVKTVPGFLIEPFYVYYKNNLGGGDISSQGLGTPKHGNQTRHTVGGRVAMKKGYFDLS
                     SEVVYQFGQMGDTAASASSLCGDPGGEGKCLHINAWATRNWFGYTAFNLPGKPRIAFN
                     FDYASGDGRANCTLAATSGDCKTANTFENFFPTNHIHMGYMDVMAWKNMMSLSGNFQA
                     RPSSNDHVEIWYTNLHLANARDNWYRASQGVYVFSQPDNTETHIGDEIDFVWTHFFMN
                     GMVAFQTGYGHLWPGSYIARNLGRRAVGQDWAYAQLWINF"
     gene            complement(2122..2421)
                     /locus_tag="VN647_01550"
     CDS             complement(2122..2421)
                     /locus_tag="VN647_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWG95740.1"
                     /translation="MFGLGASEVLIILVIAFLLFGPKQLPEVGRQVGKAIKGFKDTAE
                     DLRKSVEPELNIIQQEVKMVEQDFQASMKEAEEEITAAGSQSEENPQSSSKSSPI"
     gene            complement(2491..2778)
                     /gene="tatA"
                     /locus_tag="VN647_01555"
     CDS             complement(2491..2778)
                     /gene="tatA"
                     /locus_tag="VN647_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWG95741.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKLPQLGEGLGKAIKGFKKSV
                     HEADAIDVTPDEQSQQAQSQAASSNNQVNTQSEPTPSPAPHATPSGQVKHG"
     gene            complement(2904..3986)
                     /gene="mnmA"
                     /locus_tag="VN647_01560"
     CDS             complement(2904..3986)
                     /gene="mnmA"
                     /locus_tag="VN647_01560"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWG95742.1"
                     /translation="MTRPTVLLGMSGGVDSSVAAALLVRQGYEVHGITLQVWEHEDET
                     VSASKKWQDRGCCKIGIAKHVAKLLNISHEVIDTRHIFRKGVIDDFLHGYTSGMTPNP
                     CVRCNERVKILGLMDLAASRNFNYIATGHYARLQNYHGTSVLARATDDRKDQTYFLYR
                     LNPHWLPKLLFPLGSLAKSDVWREAEVLGLPADELKESQEICFVTQGDYRTFIEKEVP
                     EAKKPGLFIDETGQPLGRHDGIAFYTPGQRRGLGIATGQRLYVQQVHPATNTVMLGPE
                     ASLRRQECTVSDLNIFVPHRLESPAEVHVKVRYATPPTPAILSPVTSSDIRIQFREPQ
                     RALSPGQSAVFYEGDHVLGGGIIQPF"
     gene            complement(4023..4469)
                     /locus_tag="VN647_01565"
     CDS             complement(4023..4469)
                     /locus_tag="VN647_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWG95743.1"
                     /translation="MWALGDKHAQDTNNNDNFTFLAKGVDFKGILTFDGTIRIDGRVE
                     GEVHTTGTLIIGEQAVIKGTLSAGTLMTSGKVNGTVTATAKIQILKPGVLIGDIRTPG
                     ISIEDGAHFHGMCDMGDHKWVDETLTPSKNVHDLASHRTKARAVDL"
     gene            complement(4593..5087)
                     /locus_tag="VN647_01570"
     CDS             complement(4593..5087)
                     /locus_tag="VN647_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWG95744.1"
                     /translation="MWKDKQDGRRSDEVDTESNGLSLEPIRHDPGQDVSAFVGKGVVF
                     KGTITYSGTVRIDGSLEGEIHTDGILLVGEEAVITAKVTAGTIVCKGKITGDIHAKDK
                     MKLRAPAIINGGVTTPMLSMEEGVLFNGTLEMEQAAPASQRDTTHLHSISIPSEPIIR
                     RVSA"
     gene            complement(5262..6110)
                     /locus_tag="VN647_01575"
     CDS             complement(5262..6110)
                     /locus_tag="VN647_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWG95745.1"
                     /translation="MEKKALGKGLGALLPSSKTSQPTEPTDVQRIRIENIVPNRYQPR
                     HTFSQEELAELVASIKESGVLQPIMVRRKGDGIYELIAGERRWRASKEAGLETIPVVI
                     RNCSDQESLLLALVENLQREDLNPMETARAYSRMMNEFGLTQDAIAVKVGRDRSSVAN
                     FVRLIHLHPEVQEFVEGGTLTTGHAKALLGLQSTEEQLRIGKMVAAGTLSVRETEKLV
                     ELSLVGKKRQPKSPRNSPWTDLETRLQKHLGTKVTIHPQKQGGKIVIHYFSPDELDGI
                     VDTLLS"
     gene            complement(6088..6864)
                     /locus_tag="VN647_01580"
     CDS             complement(6088..6864)
                     /locus_tag="VN647_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWG95746.1"
                     /translation="MGKVIAVANQKGGVGKTTTAINLSSAIALEGKSVLLIDMDPQGN
                     ATSGLGIDHGALKGTTYSCLVKTSTIQESSMQTSIPGLFLLPTNSDLAGAEVELVNID
                     GREGHLRTILSEIKETYDFVFLDCPPALGILTINALTAADSVLIPVQCEYYAMEGLTR
                     LIGSIDLVRQSFNSNLDLEGIVLTMFDSRNTLSRQVVDQIRSHFVDKVYQAVIPRNVS
                     LAEAPSYGRPGILYNVASAGAQAYVTLAKEFIIHGEKSPR"
     gene            complement(6910..7569)
                     /gene="rsmG"
                     /locus_tag="VN647_01585"
     CDS             complement(6910..7569)
                     /gene="rsmG"
                     /locus_tag="VN647_01585"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWG95747.1"
                     /translation="MEQASSPSLLLQTSSTEIGVPLSTEQVQQFMVYLKHLQLWNQSF
                     NLTGITLHDEIVIKHFVDSLGALRADDIKVGAKLLDVGTGAGFPGIPLKIARMDLDIT
                     LIEPARKKLSFLHYIIGLLRLESVDIFDGTLERFLDERISHSSFDYLTTRALKHGLIL
                     HEGARLLRPGGKVILYSSQPIHQSDFSSNWLLMSAYTFHLQKGYGQRVISVVTPSAQI
                     T"
     gene            complement(7601..>9361)
                     /gene="mnmG"
                     /locus_tag="VN647_01590"
     CDS             complement(7601..>9361)
                     /gene="mnmG"
                     /locus_tag="VN647_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HWG95748.1"
                     /translation="HLVKEIDALGGEMGRNTDRSGIQFRFINTSKGPAVRALRAQCDK
                     SLYRMAMQESLSSQKHLTLAEGVVDRLLVAGGAVTGIVTGSGERIDGKAIILTSGTFL
                     KGLIHIGLSHFPAGRAGEASAEHLSDCMRDLGFEIGRLKTGTPPRLDKATIDFSVMIP
                     QPGDSPPPPFSYRTQHITTRQVLCHLTYTSRETHDIIQGNLDRSPLYSGIIESTGPRY
                     CPSIEDKVVRFAEKERHQIFIEPEGLNSTEFYPNGISTSLPVDVQVAMLKTIPGLEHA
                     KILKPGYAIEYDYFPPRQLYRTLETKHVAGLYHAGQINGTSGYEEAAAQGLVAGINAV
                     LKLRERAPLILDRSQAYIGVLIDDLITKDAYEPYRMFTSRAEYRLLLRHNNADLRLMS
                     VGYDVGLIPAEAYERFLRKKHLIEKEIEHLNTTRPSFTEEIRLQTKDTYLKNASSAIT
                     MAQLLRRQESNYQELLRVFGMPFIQENEIAEEVELQIKYEGYVRRQIQQVEKFKKLEQ
                     KLIPHMFNYNMVSGFSREVREKFNRVRPETVGQASRISGVTPAAISLLLVAIEKSRRQ
                     VPPAQQNSVIKFERNPNLPY"
BASE COUNT         2150 a         2272 c         2507 g         2432 t
ORIGIN      
        1 gacgccgggt ttttctttcg gccgccggat catttaccga tagaatttcc tcggtttcct
       61 gtgacgcaga attatggcga acttcaggag cgattcgttc tagcggccat gtagacggtg
      121 gaatttctcg tgtaagaggg gcgtttctcc tcggacaggc cgacatgaaa gctaactagt
      181 gcatgaaatg ctgagcataa tctttgtgag ccgatgagga agcatcgagg tagcctaaaa
      241 attgatccag agttgggcat aagcccaatc ttgcccgacg gctcgccgtc ctaaattccg
      301 tgcgatatag gaaccaggcc atagatggcc gtagcctgtt tgaaaggcga ccatgccgtt
      361 cataaagaag tgtgtccaga cgaaatcgat ttcgtcaccg atatgagttt cggtgttatc
      421 gggttgtgag aacacgtaaa cgccttgact tgcgcgatac caattgtctc gagcattggc
      481 caggtgaaga ttcgtatacc agatttcgac gtgatcattg gatgaaggtc tggcttggaa
      541 gttgccggac aagctcatca tgtttttcca tgccatgaca tccatgtagc ccatgtgaat
      601 gtgattggtc ggaaagaagt tttcaaacgt attggcggtc ttacaatcgc cagaggtggc
      661 ggctaacgta caattcgcac ggccgtctcc ggaggcatag tcaaaattga aggcgatccg
      721 aggtttccca ggcagattga atgctgtata gccgaaccaa tttctggttg cccatgcatt
      781 gatgtggaga cattttcctt cgcctccagg gtccccgcag aggctgcttg cactggcagc
      841 ggtatctccc atttgcccga actgataaac cacttcgctg gagagatcga agtagccttt
      901 cttcatggct acccgtcctc cgacggtatg acgagtttga ttgccgtgct tcggagtgcc
      961 gagaccctga gaagaaatat ctcctccacc caagttgttt ttgtaataga catagaaggg
     1021 ttcaatgaga aatccaggca cagtcttgac ctgattgtaa aagatgaaca agtcggcatc
     1081 accggtggcg tttcttgtct gacctgtccc gacgatgttt gcttgaccac tgccgacggc
     1141 agccccttgg aagaggtctg tttctgctgt ccgaaaccag cctgcatggg tatccatgat
     1201 gctgttactg tactgcacca tgactccgtc aaacgagaag cctgtgttcg cccaatcgaa
     1261 gtggccgaac aagctctgat cgccgaacac cagatactgc cgaccggctt tgagacccag
     1321 accctgtatg ccggcaaaat tacgaatcag catgtagccg gctcgcactc caagtgtgca
     1381 gaccggcctc ccgccaggat taggcgtgga cccacaggtg tggatcgaag ggtctccgtt
     1441 gttcccggcg cctccggcgc cgacaggcga tccattacca ccccaggtgc gagaatcaat
     1501 gaattcgagg taaaaattga cgtcgggtga caggtcatag ccgatgccca gacgggtcat
     1561 ctgttggacg aattgatcgt tggcgttgcc ggtcaggtta tttggggcaa tgccggggcc
     1621 gttacattct ccggacgctc ctatccctcc tccgaagcag acgttattgc ggaattctgg
     1681 ccgtacacgg aggtcggcgc gcatccagag gtttttgagg tcgaagtttc tgccgatctt
     1741 cgggtcgaac agttcgtagg cttctttttg agggatacca cgtccgatga caataggatt
     1801 ggtaatcttt tccccttcgg gcagctcgaa agcgctctcg gctggaccgc agaaggtcaa
     1861 tagcccgaca acagccctca tggctgtgag gaagatcgtc ttccattgtc ctcgaactgt
     1921 cggtttagga agcgtccttg ctgtattcat tctctgaaaa ggaagggagt cgcgcaggcc
     1981 tttcagcacg tgggaaaaaa tgcgtaacga gctgcctgta tgtcatcgtc aatgcgaggg
     2041 gccgatgaga ggggggaaaa atggagagga gtttatgttg ccggtgtttt tgtcgtgaaa
     2101 ccaacgcgct gatcattccg gttatatggg actcgacttg ctagaagact ggggattctc
     2161 ttcggattgg ctacccgcag cagtgatttc ttcctcagct tccttcatag aagcttgaaa
     2221 gtcctgttcg accattttga cttcttgttg aatgatgttg agctccggct ccaccgattt
     2281 gcgcaagtcc tcggccgtat ctttaaagcc cttaatagct ttgccaactt ggcgtccgac
     2341 ttcgggaagt tgtttggggc caaataggag aaaggctatc accaaaataa ttaagacttc
     2401 gctggctccc aatccaaaca tgattcaggg tagtttatgg taaggccgtt aatctccccg
     2461 ccgtccaatc gtcacaggac actcaggccc ttacccgtgt ttcacctgtc ccgatggcgt
     2521 cgcgtgggga gcgggcgatg gcgtgggttc tgattgggta ttgacctgat tattagacga
     2581 ggcggcctgt gactgggctt gctgtgactg ttcgtccggt gtcacgtcaa tggcatcggc
     2641 ctcgtgtacg gacttcttga agcctttaat ggctttgccg agaccttctc ccaattgggg
     2701 cagtttaccg gccccgaaaa tgatcaagac aatgatcaag atcagcaata attccatcca
     2761 tccaaatgaa ccgaacatta aaacctcaga tgaatgcggt gagtatggac tttcaatggg
     2821 aatggggaca cttttctccc catatcttca gaggctatag gaaaattaca ggacaagtca
     2881 agaagaagaa aaggccattg gcgctaaaac ggttggataa tccctcctcc gaggacatgg
     2941 tctccttcat agaagacggc agattggccg ggacttaatg ctcgttgagg ttcccggaat
     3001 tgaatacgga tgtctgagga ggtcacgggt gacagaatgg caggtgtcgg gggtgtcgcg
     3061 tagcggactt tgacgtgaac ttcagctggg ctctccaatc gatgaggcac aaagatattg
     3121 aggtcgctta ccgtacactc ttgtctgcgt agggatgcct ccgggccgag catgacggta
     3181 ttggtcgcag ggtgcacctg ttgaacatag agccgttgtc ctgtggcaat gccaaggcct
     3241 cggcgttggc ctggtgtgta gaacgcaatt ccgtcgtggc ggcccaaggg ctgcccggtc
     3301 tcatcgataa agaggccggg cttcttggct tcggggacct ctttttcgat gaaggttcga
     3361 tagtctcctt gagtgacaaa gcagatttcc tgactttcct tcagctcatc ggccggaagc
     3421 cccaaaacct cggcttctct ccagacgtcc gatttggcca ggcttcccag agggaagagg
     3481 agcttgggta gccagtgagg attgagacgg tagagaaagt aggtttgatc cttgcggtca
     3541 tcagtggccc gcgcgaggac ggaggtgccg tgatagttct ggagacgagc gtaatgtcct
     3601 gttgcgatgt aattgaagtt gcgcgaggct gcgagatcca tgagtcccag aatctttact
     3661 ctctcgttgc agcggacaca gggattgggt gtcatgccgc tggtatagcc gtggagaaaa
     3721 tcgtcgatca ctcctttccg aaagatgtgg cgggtatcga tgacctcgtg ggagatgttg
     3781 agaagtttgg cgacgtgctt agcgatgccg atcttacaac aaccccggtc ttgccacttt
     3841 ttggatgctg atacggtttc gtcctcgtgt tcccatactt gaagggtaat gccgtgcacc
     3901 tcgtaacctt ggcgaacgag caatgcagct gcgacggagc tatcaactcc gccgctcata
     3961 ccgagaagga cggtcgggcg tgtcatgagt ccggcattgt accgggaaga gtcgataaag
     4021 ggctagaggt ctaccgcccg agctttggtt cgatgggatg ccaaatcatg aacattcttg
     4081 gatggcgtga gggtttcatc gacccattta tgatctccca tgtcacacat accatggaag
     4141 tgagctccat cctcgattga aatacccggt gtgcgaatgt caccgatgag aaccccaggc
     4201 ttaaggattt gaatttttgc ggtggctgtg accgtaccat tgaccttacc gctggtcatc
     4261 agggtgccgg ctgaaagagt gcctttgata actgcctgct ccccgatgat cagagtcccg
     4321 gtggtgtgga cttcgccttc aacgcggcca tcgatgcgga tggtcccatc aaaggtcagt
     4381 atgcccttga agtcgactcc ttttgccagg aacgtaaaat tatcgttgtt gtttgtgtct
     4441 tgagcatgtt tgtcgcctag agcccacatc agttttcctt actccttctg acaacagccg
     4501 atgagggatt accgtcaatt gggtcgaaca gaagggagac gcattcggtg agaagtagtg
     4561 tccccctatt tattgtttag tgtatcacat ggctatgctg agaccctcct gatgatgggc
     4621 tcactcggaa ttgagatgga atggagatgt gttgtgtcac gttgcgaggc gggtgctgct
     4681 tgttccatct ctaaggtgcc gttaaagagg actccttctt ccatggagag catgggtgtg
     4741 gtgactccgc cgttgataat tgcaggagcc cgcaacttca ttttgtcttt ggcgtggata
     4801 tcccctgtaa tcttcccctt acagacgatg gtgccggccg taaccttcgc cgtaataacg
     4861 gcttcttcac caaccaacag gattccgtcg gtgtggattt ctccctcgag actcccatcg
     4921 atccgtacgg tcccgctgta ggtgatggta cccttgaaca cgacgccttt tcccacgaaa
     4981 gcgctgacat cttgcccggg gtcgtgccgt ataggttcca ggctcaaccc attgctctca
     5041 gtatcaacct cgtcggaacg tctgccgtct tgtttgtcct tccacataca ctctcctcct
     5101 ctcgagattt aggatagcgg gatccgatga aagggcagag gccttatcgt tagggaacat
     5161 atcggtctgt tccgatgaga tatttagcgt tcatatgctt aaaaggcaat aagatctgtt
     5221 ttttaggcga ccaggaggca caccggagca aggctagggg gttaactcag gagtgtgtcc
     5281 acaataccat cgagttcgtc aggggagaaa tagtgaatga cgattttccc tccttgcttt
     5341 tgcgggtgga ttgtcacttt cgtgccaaga tgtttctgta atctggtctc gagatcagtc
     5401 cagggtgaat tgcggggact cttgggctgc cgcttctttc ctacaaggga gagctccacg
     5461 agtttttctg tctctctgac agaaagagtg ccggcggcga ccattttgcc gattctcagc
     5521 tgctcctccg tggattggag gccgagaaga gctttcgcgt gaccggtggt cagggttccc
     5581 ccttcgacga attcctgtac ctcagggtga agatggatca gtcgaacgaa gttggcgacg
     5641 gaggaacgat cacgaccgac ttttacggca atcgcgtctt gcgtaaggcc gaattcattc
     5701 atcatgcgcg aatacgcccg tgcagtctcc atcggattca agtcttcccg ttggagattc
     5761 tcaactaagg ccagtaagag agattcttga tcactgcaat tgcgaatgac gacagggatg
     5821 gtctccaggc cggcttcttt cgaggcgcgc catcggcgtt ctccggcgat caattcgtaa
     5881 atgccgtcac ccttgcggcg aaccataatc ggctgaagca ccccgctttc tttgatggaa
     5941 gcgaccagtt cagcaagttc ttcctgggaa aaagtgtgcc gcggttgata gcgattgggc
     6001 acgatattct cgatccggat tcgttggaca tcggtcggtt ccgttggctg gctcgttttg
     6061 gaggatggaa ggagggcgcc aagtccttta ccgagggctt ttttctccat ggattataaa
     6121 ctccttagct aaagttacgt atgcttgcgc gccggcagat gccacgttgt acaaaattcc
     6181 aggtcggccg taactcggag cttctgcgag cgagacattg cgcggaatga ctgcttgata
     6241 gactttatcg acgaagtgag accgtatttg gtcgacaacc tgcctggaca aggtattgcg
     6301 agagtcaaac atcgtcaaga cgatgccttc caggtctagg tttgaattaa acgattggcg
     6361 gacaagatcg atgctcccaa tgagtctggt caggccctcc atagcgtaat attcacattg
     6421 gaccggtatg agaactgaat ctgccgcggt aagagcattg atggtgagaa ttccaagagc
     6481 tggagggcaa tctaaaaaaa caaagtcata tgtttccttg atctcactga ggatagttct
     6541 gagatgaccc tcgcgcccat cgatgttgac gagctcaact tcagccccgg caagatccga
     6601 gttggtcggc aagagaaaga gcccaggaat tgatgtctgc attgaagatt cttgaattgt
     6661 gctggtttta acgaggcaac tgtaggttgt gcccttcaag gccccatggt caatgccgag
     6721 gccactcgtc gcgtttcctt gtgggtccat atcgatgagc aatacggatt ttccttctaa
     6781 cgcaatggca gaggaaagat ttatcgccgt cgtcgtcttg ccaactccgc ctttttgatt
     6841 cgcgaccgca ataacctttc ccatcgaaga gctcctacag cagatttgtt ctacgtggaa
     6901 catttaagat taggtgattt gagctgaagg ggtaacaacg gaaattactc tctgtccata
     6961 tcctttctga aggtgaaatg tatatgcact catcaataac caattggaag aaaaatctga
     7021 ttgatgaatc ggttgtgacg agtaaagaat gactttccct cctggtcgaa ggagtctcgc
     7081 accttcatgc aggatcaagc catgtttcaa cgcgcgggtg gtcaagtagt caaatgatga
     7141 atgggaaata cgttcgtcca agaatctctc tagagttccg tcgaatatat cgacgctttc
     7201 aagtcgtagg aggcctatga tgtagtgaag gaaggagagt ttctttctcg ccggctctat
     7261 aagagtaata tccaggtcca ttctcgcaat ttttagagga attccaggaa atccagctcc
     7321 tgtaccgaca tcgagcagct ttgccccaac ctttatatca tcggccctga gtgcgccgag
     7381 ggaatccaca aagtgtttga taacaatttc gtcatgtaag gtaatacctg taagattgaa
     7441 tgattgattc cagagttgga ggtgcttaag atacaccatg aactgttgca cttgttcggt
     7501 actgagtgga accccaattt cagtcgagct tgtctgtaac agtagggaag gtgaactcgc
     7561 ttgttccacg tggaacaatt acgctgatgc ggaagtgagg tcaataggga aggttggggt
     7621 tccgttcgaa ttttatgaca ctattttgtt gagcaggagg aacctggcgt cggctctttt
     7681 ctatcgcaac tagaagcaaa gatatggcag caggagtcac gccggatatt ctcgatgcct
     7741 ggcccactgt ttccggtctt actctattga acttctctcg aacttctctg gaaaatcctg
     7801 aaaccatgtt gtaattgaac atatgaggaa tcaatttttg ctcaagtttc ttaaatttct
     7861 ccacctgttg gatctgacga cgtacataac cttcgtattt gatctggagt tctacctctt
     7921 cagcaatttc attttcctga atgaacggca ttccaaaaac tcttaagagt tcttgatagt
     7981 ttgattcctg ccgacgaaga agctgcgcca tagttatggc agacgaggcg ttcttgagat
     8041 acgtgtcttt tgtctgcaac cggatttcct cggtgaatga tggcctggtt gtattcaggt
     8101 gttcgatctc tttttcaatc agatgtttct tccgcaaaaa tctctcatat gcttccgcgg
     8161 gaataagacc aacgtcatat ccaaccgaca taagtcgaag gtctgcgttg ttatgacgga
     8221 ggagcaatcg atattctgct ctcgacgtga acattcgata aggttcgtag gcatcttttg
     8281 tgatgagatc gtcgatgagt accccgatat aggcttggga tcggtcgagg atgagaggag
     8341 ctctctcccg caacttcaga acggcattga taccggcaac taaaccctgt gccgcagctt
     8401 cttcataccc agaagtcccg ttgatttgtc cggcgtgata gagtcctgca acatgttttg
     8461 tttcgagcgt tcgatagagt tgacgaggag gaaaataatc gtactcaatc gcatagccag
     8521 gcttgagtat tttggcatgt tctaatccag ggatcgtttt caacatagcg acctggacat
     8581 caaccggtag gctggttgag ataccgttcg ggtaaaattc tgttgagttg aggccctcag
     8641 gctcaataaa aatctgatga cgttccttct cggcaaaacg tacgacctta tcctcgatgg
     8701 aaggacagta ccgaggcccc gtagattcga tgattccgct gtacagaggc gagcggtcga
     8761 gattcccttg aataatgtcg tgtgtctccc ggctggtata ggtcaagtgg cagagaactt
     8821 gtcgggtcgt gatgtgttga gtgcgatacg aaaaaggggg aggaggacta tcgccgggct
     8881 gcgggatcat caccgaaaaa tcaatcgtag ccttgtccaa tcggggagga gttcccgtct
     8941 tgagtcgtcc gatctcaaat ccaaggtccc gcatgcaatc tgaaaggtgt tcagccgagg
     9001 cctctcccgc acggcccgcc gggaaatggc tgagcccaat atggataaga cccttaagga
     9061 aagtgcccga tgtcaatata attgccttgc catcgatccg ttcaccagag cctgtcacga
     9121 tcccggttac cgcacctccc gccacgagca gtcgatccac gactccttcc gctagagtaa
     9181 ggtgtttctg cgagctgagg ctttcctgca tggccatgcg atacagcgat ttatcgcatt
     9241 gggcgcgcaa cgcccgcacc gccggtcctt tgcttgtgtt gatgaatcga aattgaatcc
     9301 cggaacgatc cgtattccgc cccatctctc cgccgagcgc atcgatctcc ttcacgagat
     9361 g
//
LOCUS       DATNDD010000073         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1526341, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000073 DATNDD010000000
VERSION     DATNDD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526341"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VN647_01595"
     CDS             complement(<1..569)
                     /locus_tag="VN647_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95749.1"
                     /translation="MQVFTSPLALWSFLEARSGTIPSRPAYPCLFALTFVLLGLVLTS
                     SPVYALEKYGRPLPSLEGENKEAREAEETLFGGYFLTAAFLSNPTFAARPDNTGLVGL
                     RHMAHLETDLYKQYLTFYTDQNFFSDRTKGWITLTEWDMTYALTGLIDRWGWRIQYER
                     DAPLDRSGIQQVYADALVTGRFQAAEDLS"
     gene            complement(668..2083)
                     /locus_tag="VN647_01600"
     CDS             complement(668..2083)
                     /locus_tag="VN647_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWG95750.1"
                     /translation="MIDQTTPPPALSGPPVSRWRGCFPEFRRDTLGFLLRCHAYGDVV
                     KLPMGLVVDLLLGQRDAAMYVLNHPADIKHVLVTNQDNYRKAPVPPVESRVFGQGVLH
                     TEGEPHHRQRRLFLPFFHGNHVTAYTDLITGKAHDLVGQWQNGTTVDIEHEMAHLTLS
                     VIWRLLFGQDVGSEADTIREAITIGQSLIKLQYDSLLASCTPLWVPTKLHRRFTRGHR
                     SMEAMIRRMIEERRTASHQNDDVLALLLAARDTDGRLLSDKEIHDELVTFLLAGHETT
                     ANALTWTWFLLAQFRPVQERLMCELNEALGGRLPTAADVPRLRYTKMVWEESLRLYPP
                     AWSLHTRVAHAKDRLPSGAVLPSRAWVFISPWSLHRNERWFPDPNRFDPERFSEEAIR
                     TRPPFSYIPFGAGGRRCLGESFAELEGLLILATIASKVRLRLVSGQTIRPEPVMTLRP
                     DVSISMIVQRVAITEPLSAHA"
     gene            complement(2133..>2559)
                     /locus_tag="VN647_01605"
     CDS             complement(2133..>2559)
                     /locus_tag="VN647_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG95751.1"
                     /translation="CMGKLSDRVGHRWVLASGAAGLALGTVPFFLWLSSGHLALIVAA
                     QVLITVFVSAYMGPFFAIVATLFPVAQRYTGLSASYNIASALFGGTAPLMATVMMERS
                     GSVLAPGWYVSLCAVLSLIALSTIREELKGTVEVPAGTH"
BASE COUNT          647 a          717 c          717 g          478 t
ORIGIN      
        1 caggaaagat cctcggcggc ctgaaaacgg ccggtcacca aggcatccgc atagacttgt
       61 tgaatgccgc tacggtcgag gggcgcatca cgttcatact ggattcgcca gccccaacga
      121 tcaatcaatc cggtgagagc gtaggtcata tcccactccg tgagggtgat ccagcctttc
      181 gtccgatcgg aaaagaaatt ctgatcggta tagaaggtga gatactgttt atacaggtcc
      241 gtctccaagt gggccatgtg tcgcaacccc accaggccgg tattgtcggg gcgggctgca
      301 aaagtggggt tggaaagaaa cgcagcggtc aggaaatatc caccaaataa ggtctcctcc
      361 gcttccctgg cctccttgtt ttctccctcc agagagggga gcgggcgacc atatttctcc
      421 aaagcgtaca caggagacga agtgagtacc agccccagca gcacgaacgt gagtgcaaaa
      481 agacacggat aagccggtcg gctgggaatt gttcccgacc gagcctcaag aaaagaccaa
      541 agtgcgagcg gtgatgtaaa tacctgcatc aagtacgtca tagtgaggcc ggcaacaatc
      601 ggacgcgact tgtaccatat caaaagagag atacctagca gggatgaaat ggagcgagcg
      661 gagggattta ggcatgagcg gaaagcggct ccgtaatagc aacccgttga acgatcatcg
      721 agatcgaaac atcggggcgt agcgtcatga ctggttccgg tcggatcgtc tgaccactaa
      781 ccaggcgcaa ccgaactttg gacgcaatcg tcgccaagat caacaggcct tccaactccg
      841 caaaggactc tccaaggcac cgacgtccac cggcgccgaa agggatgtag ctgaacggtg
      901 gacgggtccg gatcgcttct tctgaaaatc gctcaggatc aaatcggttg ggatccggga
      961 accatcgctc attacggtgc agactccaag gactgataaa gacccaggca cgggatggca
     1021 gcacggcacc tgacggaagc cggtccttag catgggcgac acgggtatga aggctccacg
     1081 cgggaggata gagccgaagc gactcttccc agaccatctt cgtataccgc aagcgaggca
     1141 catcagcagc agttggaaga cggccaccta acgcctcatt caattcgcac atgagccgtt
     1201 cctgtaccgg tcgaaattgc gccagcaaaa accaggtcca ggtcagcgca ttagcggtgg
     1261 tttcatgacc ggcgagcaag aaggtcacaa gttcgtcatg gatctcctta tcactcagca
     1321 gacgtccatc cgtatctctc gcggcgagca acaacgccag cacgtcatca ttctgatgag
     1381 acgccgtacg tctctcctcg atcatccgcc gaatcattgc ttccatcgat cggtggcctc
     1441 gggtgaaccg gcgatggagt ttcgtcggaa cccacagggg agtacagctc gccaacaggg
     1501 agtcatactg taatttgatt aaactttgcc cgatcgtaat ggcttcccgg attgtgtcgg
     1561 cttcggaccc gacgtcctgt ccaaagagta ggcgccagat cacagacagg gtcaggtgcg
     1621 ccatctcatg ttcaatatca accgtcgttc cgttttgcca ctgtcccacg aggtcatgag
     1681 ccttgcccgt aatcaaatcg gtgtacgcgg taacatgatt tccgtgaaag aaggggagaa
     1741 acaaccgtcg ctggcggtga tggggctcgc cttccgtgtg gagcacacct tgaccaaata
     1801 cccgagattc gacaggaggg acgggcgcct tccgataatt gtcttgattg gtcaccagca
     1861 catgcttgat gtcggccgga tggttcagca catacatggc agcgtcgcgc tgccctaaga
     1921 gaaggtcgac gacaagaccc atcggaagtt tgacaacgtc cccataggca tgacagcgca
     1981 agagaaaccc gagagtgtca cggcgaaatt cgggaaaaca tcctcgccag cgggagactg
     2041 gtggaccgga aagggcaggt ggcggagtcg tctgatcgat catgttgcct tcgtgtcgct
     2101 catgaaagct atcgtatgtg tcgtggccac cgtcaatggg ttccagccgg aacctcgacg
     2161 gtccctttca gttcctcacg aatcgtggac aatgcgatca acgataggac ggcgcacaag
     2221 ctgacatacc atccaggagc aagaacactc ccgctccgtt ccatcatcac tgtggccatc
     2281 aacggtgccg tcccgccgaa cagggccgag gcaatattgt aggaggctga aagacccgtg
     2341 tagcgttgcg cgactggaaa gagagtcgct acgatggcaa agaacggacc catataagcc
     2401 gacacgaaca ccgtaatcaa gacttgcgcc gcgacgatca acgcgagatg gccgctgctc
     2461 aaccacaaaa agaatgggac tgtgccaagg gccagccccg ctgccccgga ggccagtacc
     2521 caccgatgcc cgactcgatc agacagcttg cccatgcac
//
LOCUS       DATNDD010000074         5205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3911791, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000074 DATNDD010000000
VERSION     DATNDD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911791"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VN647_01610"
     CDS             complement(<1..1164)
                     /locus_tag="VN647_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95752.1"
                     /translation="MSMKGHVARRWKQFVVAGAMTALVAGMGSTLPSAYAGGTIKADD
                     DKWISIGMGIRTSFNSLENGAVNGHAYSNEFTINNARIYINGQVHKYVKFEFNTDCFN
                     CNIVGTQQIGGGTGFGANSSIGLLDAIGKFEFNEMVNIWVGRMLLPSERGELNGPFYH
                     ATFDGFRTPLFPADFSGNFNAGGGGGAGLYGRDNAATFWGKVHPFGTHLLYVATVSQG
                     LRNGPNTGNSLMYTGRLQWNLLNDEPSPGYYTAGTYYGTAGDILAIAGNVQHQKDGAG
                     NAGLAITPGNFTGTNVSDFTGLSVDLLFEKVLPNNMGVFTFNGEFKRFFANYGTGAFT
                     NNPGTCFCVFNGHSWTAYGLYLIPNEIGIGRFQPYVRFTSVSPLYSSMRQEFEY"
     gene            complement(1465..2100)
                     /locus_tag="VN647_01615"
     CDS             complement(1465..2100)
                     /locus_tag="VN647_01615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWG95753.1"
                     /translation="MAIRLGDDAPNFTAETTEGTINFHEWLGNGWGILFSHPKDYTPV
                     CTTELGAVAKISPEFKKRGVKVIAVSVDPMDSHKGWINDINETQKTTMNYPIIADPDK
                     KVATLYDMIHPNAIDNMTVRSVFIIGPDKKVKLTLTYPASCGRNFDELLRVIDSLQLT
                     SKFKVATPANWKEGEDCIITPAVGDAEAKQLFPKGFTTVKPYLRYTPQPNR"
     gene            2283..2648
                     /locus_tag="VN647_01620"
     CDS             2283..2648
                     /locus_tag="VN647_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWG95754.1"
                     /translation="MAGMDTARTKLHISKDPMYLMLREGCIKEFNVKKAAGDKCDLRG
                     CDLRGLDLRGLDADGLDFSDCYFRQSDLRGVDLSKTKMDGASINACKISGVLFPAELS
                     ASEIELSLLHGTRMRYSVK"
     gene            complement(2656..>5205)
                     /locus_tag="VN647_01625"
     CDS             complement(2656..>5205)
                     /locus_tag="VN647_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWG95755.1"
                     /translation="SPSNDAKQSPPNEPKKMMPVEIELLEITQGILDYRDDSKLKPVL
                     IDVVPIQIVLRDFSTVPSQESENAYAFKAEIGKGEQVAWEGTFSLEPVESDGKLSVSG
                     IKLQTLYQAVRDQFQFDVPQGILGLSASYHFDLRGQVPQATVKNGAVSIQNLALVERG
                     GIEPVVKIPAFGVEGIQLDLQKQTIDVSKVHSADARFEAWMDPDGAFNYQQLFTPVGG
                     RGAEEKLPAATAKPEKPAKPWSIVVDEIGIKNYGASFEDRTLGRPGHVEVDAMNVTVK
                     DIQIPFKKSLPIDLSLKLNETGSIDVKGKVAAEPLRADTELNLAHIEIRPFQPYLDRF
                     LNADVLDGAIDLNGTVHFAKEHPNEPMMRFQGNLAVNRLAVADRKDLDNVLTWKALNV
                     RQIALDVEPTVVKIAEVVWQEPSIRMVIDAEGQLNLSRLAVSPPPGDQTPAPQKPEGE
                     KSRAAAKDPVSVTIDQVKLGKLTATFQDLSIEPHVRTSLTEFGGTIKGLSSKQLKKAD
                     VNLRGKVGRAAPLKIAGKINPLSGDAFTDLVVTLGAMDLTSVGPYSGKYAGYGLSKGK
                     LSLDLKYKVSQKVLEAENLVHVDQLTFGEKTNSPDATSLPVPLVVALLQDRRGLIEID
                     MPIRGNLNDPDFKYGKVVISTLLNLLGKVVASPFALMGKLVPGGGGEEDLQFIEFQPG
                     SDSLADRELTKLEALEKALGERAGLRLDIKGTTDSTLDAAALQRMKLRDQLFAMSGGA
                     KPDQEELSPKEEQRLVEKLYAKLPPPDPSATPAEPTQPTVADMKRQLAAAIHISTSEL
                     ETLARQRAEAIRHHLLESGKLTEERVALLDTVVAESDHKKVRTQLSLSAGP"
BASE COUNT         1154 a         1498 c         1331 g         1222 t
ORIGIN      
        1 atactcaaat tcctgccgca tggagctgta cagtggactg acgctcgtga accgcacata
       61 gggttggaac cgtccgatgc cgatctcatt gggaatgagg tacaggccgt aggccgtcca
      121 agagtggcca ttgaatacgc agaaacaagt gccagggtta ttggtaaacg caccggtccc
      181 ataattggcg aaaaaccgct tgaattcgcc gttgaacgta aacacaccca tgttgttcgg
      241 gagaaccttt tcgaacaaca gatcgacaga aagtccggtg aaatccgaca cattagttcc
      301 agtgaagttc cctggtgtaa tggcgagccc tgcgttccct gctccatcct tttggtgctg
      361 cacgttaccg gcaatcgcga ggatatcccc ggcggtccca taataggtac cggcggtgta
      421 atagccgggg ctcggttcat cgttcaacag attccactgc aaacgccccg tgtacatcaa
      481 gctgttgccc gtattgggtc cgttccgtaa cccttgagac acggtcgcaa catacaataa
      541 atgggtgccg aaggggtgaa ccttgcccca gaaggtcgcc gcattgtcac gaccatagag
      601 accggctcca ccaccgcccc cagcgttaaa attgccgctg aagtcggccg ggaaaagcgg
      661 cgtcctgaaa ccatcgaagg tcgcatggta gaagggaccg ttcaactcac cgcgctcgct
      721 cgggagcaac atgcgcccga cccagatatt gaccatctca ttgaactcaa acttgccgat
      781 tgcatcgagc aagccgatcg aggagttagc tccaaaacca gtcccaccgc caatctgctg
      841 cgtgccgaca atattacagt tgaagcaatc cgtattgaac tcgaacttga catacttgtg
      901 gacctgcccg ttgatgtaga tacgagcatt gttgatggtg aactcattgc tgtaggcatg
      961 accgttcacc gctccattct ctagggaatt gaagctggta cgaattccca tgccgataga
     1021 aatccatttg tcatcatcgg ccttgatcgt gccaccagca taggcactcg gcagcgtgga
     1081 tcccatccca gccaccagcg ccgtcatggc cccagcaact acaaattgtt tccaccgcct
     1141 cgcaacatgt cccttcatac tcatgccctc ccttgtgagt aataagaaac cactgcccac
     1201 ctacgcctaa tgttcaaaac cgttcacccc ctgggaagtc ggcgggagta tgcctaaagc
     1261 atcgaaatat ggcaagaaaa tttttggcac ctccctgttt caccacgtaa gactactcag
     1321 aaggttttct tctgtaaagg aaaacgaata atgtgaccaa gaatacttac cgagccagat
     1381 gcaggatgga tacagtcatg gtgagggacg aggcagaagg tgctgtcgca ccttctgcct
     1441 attggagagg gaatgattcg atgattaccg attcggctgc ggggtatagc ggagatacgg
     1501 cttgacggtt gtgaaccctt tcgggaagag ttgcttcgct tccgcgtcgc cgaccgcggg
     1561 agtaatgatg caatcctcgc cctctttcca gttcgccgga gtggcgacct tgaacttcga
     1621 tgtcagttga agagagtcga tcactctcaa caactcatcg aagtttcgac cgcaggaagc
     1681 gggataggtc aacgtgagtt tgaccttctt gtccggaccg ataataaaga ccgagcgcac
     1741 cgtcatgttg tcgatggcgt tgggatggat catatcgtac agcgtggcga ccttcttatc
     1801 cggatcggcg atgatggggt agttcatcgt cgtcttttgc gtttcgttga tgtcgttgat
     1861 ccagcctttg tgggaatcca tcggatccac gctgacggca atcactttga cccctcgctt
     1921 tttgaactcc ggcgagattt tggccacggc acccagctcc gtcgtacaca ccggggtgta
     1981 gtccttggga tgtgaaaaga gaatccccca accgttgccc agccattcgt gaaaattaat
     2041 cgtaccctcc gtcgtttcag ccgtaaaatt cggggcatcg tcgcccaatc gtatcgccat
     2101 gggatacctc cttgtatcgc gatcacctac cacaagattt ctaccatcgc cgtacaatac
     2161 tgtgaccaaa cggcgagttc aagaggcatt cgcaaggtca aggctgaggc ttccccctag
     2221 ctcaagcttc tcgcgtctca tgccgataga aacttcagaa atccttttca acggagccga
     2281 acatggccgg tatggacaca gcccggacaa aactccatat ctcaaaggac ccgatgtacc
     2341 tcatgctgcg tgaaggctgc atcaaggagt tcaacgttaa gaaagccgcc ggggacaagt
     2401 gcgatctccg tgggtgtgat ctgcgcggtc ttgaccttcg aggattagat gccgacggac
     2461 tggatttcag cgactgctat ttccggcaat ccgatctccg tggcgtcgac ttgagcaaaa
     2521 ccaaaatgga cggggccagt atcaatgcgt gcaagatttc cggcgtgctc ttccccgcgg
     2581 agctgagcgc ctctgaaatt gagctgtctc ttcttcacgg gacgcggatg cggtacagcg
     2641 tgaagtaact gtcgttcacg gtcctgccga cagcgacaac tgagtccgca ccttcttatg
     2701 atcagactca gcaacgaccg tatcgagcag cgcgactcgt tcctccgtga gctttccgct
     2761 ctccagaaga tgatggcgga tcgcctcggc gcgctggcga gccagcgtct ctaattcgct
     2821 cgtggaaatg tggatggccg ccgcgagttg tcgcttcatg tccgccacgg tcggctgcgt
     2881 gggttcggcc ggtgtcgcgg atggatcagg cggaggcagt ttcgcataga gtttttccac
     2941 caaccgttgc tcctctttcg gcgacagctc ttcctgatcg ggttttgccc cgccgctcat
     3001 cgcgaacagc tgatctcgga gcttcatccg ttgaagcgcc gccgcatcaa gcgtcgaatc
     3061 ggtcgttccc ttgatatcga gccggagtcc cgcccgctca cccaacgctt tctccaatgc
     3121 ctcgagctta gtcagttcac ggtcggccag tgagtcgctc cccggttgaa attcgataaa
     3181 ttgaagatct tcctcgccac cgccacccgg cacgagcttg cccatgagcg caaacggcga
     3241 agcgacgact ttcccgagca gattgagcaa cgtcgagatg acgaccttcc catacttgaa
     3301 atccggatcg ttgagattgc cgcgaatcgg catatcgatt tcgatgagac ccctccgatc
     3361 ttgcagcagc gctacgacca acggaacagg cagcgacgtg gcatccgggc tattcgtctt
     3421 ctcaccgaac gtcagctgat caacatgcac cagattttcc gcttccaaca ctttctgaga
     3481 caccttgtac ttcaaatcga gcgacaattt tcccttcgac aacccatagc ccgcatattt
     3541 accgctatag ggtccgaccg atgtcaaatc catcgcgcca agggtcacga ccagatcggt
     3601 gaaggcatct ccgctcagcg ggttgatctt tccggctatt tttaggggag cggctcttcc
     3661 aactttgccc ctcaggttga cgtcggcttt cttcagctgc ttcgacgaca gtcctttgat
     3721 ggtcccgccg aactccgtga ggctggttct cacgtgtggt tcaatagaca aatcctgaaa
     3781 cgttgcggtc aatttcccca acttcacctg gtcgatggtg acagacacag gatcctttgc
     3841 cgcagctcgc gatttctcgc cttcaggctt ttggggagct ggagtctgat caccgggcgg
     3901 aggcgagacg gcgagacgcg agagattgag ttgtccttca gcatctatca ccatccggat
     3961 agaaggttct tgccagacga cttccgcaat cttcaccacc gtcggctcga catccagggc
     4021 aatttgccgc acgttcaacg ctttccatgt cagcacattg tcgagatctt tgcggtccgc
     4081 gaccgccagc cgattgactg caaggttgcc ctggaatcgc atcatgggtt cgttggggtg
     4141 ctccttcgca aaatgtaccg ttccgttcag atcgatcgca ccgtccaata catcggcgtt
     4201 gaggaaccga tccagataag gctgaaaggg acggatttcg atatgtgcca gattcagctc
     4261 cgtatcggca cgcagaggtt cagccgccac ctttcccttc acatcaatgg atccggtctc
     4321 attcagtttc aatgacaggt cgatgggaag cgattttttg aacgggatct gaatatcctt
     4381 taccgtgaca ttcatcgcat ccacttccac atgtccggga cgtcccaggg ttcggtcctc
     4441 gaatgacgcg ccgtagttct taattcctat ttcatcgaca acgatggacc agggcttcgc
     4501 cggtttttcc ggcttggcgg tcgcggctgg aagtttttcc tctgctcccc tcccgccgac
     4561 cggtgtgaag agctgttgat agttgaatgc accatcagga tccatccagg cctcgaaccg
     4621 ggcatcggct gaatgcacct tcgagacatc gatggtctgc ttttgcagat ccagctgaat
     4681 cccttccaca ccaaaagcag gaattttcac gaccggctcg attcctcccc gttccacgag
     4741 agcgagattc tgaatcgata ccgctccatt cttgaccgtt gcctgaggaa cctgaccgcg
     4801 aagatcgaaa tggtacgacg cggatagacc aaggatcccc tgcgggacat cgaactgaaa
     4861 ttgatcgcgc acagcttgat acaacgtctg cagcttgatt ccggatacac tcagcttgcc
     4921 gtcggactcc accggctcca gcgagaaggt tccttcccac gccacttgtt caccctttcc
     4981 aatttccgcc ttgaaagcat aggcattctc gctctcttgg cttggaacgg tactgaaatc
     5041 tcgcagcacg atctggatcg gcacgacatc gatcaagacc ggcttgagct tggagtcatc
     5101 gcggtaatcc agaatgccct gggtgatctc cagcagctcg atttcgaccg gcatcatttt
     5161 cttgggctcg ttcggcggcg attgtttcgc atcatttgaa gggga
//
LOCUS       DATNDD010000075        15064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3932816, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000075 DATNDD010000000
VERSION     DATNDD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3932816"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VN647_01630"
     CDS             complement(<1..949)
                     /locus_tag="VN647_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HWG95756.1"
                     /translation="MATITEQYQFLNPVNGWHASESMRSKLRPRPNDGALMLPALTGQ
                     ARPLLDPKIMGEPCGSSTGKKAKRSRSKPGSLCCPSAVAAGPCGSSILVIDATLNRVK
                     RIRFRSAEELVTLTNRDFSQPYLGGRIDTITAFGGSGIEPRKFRHPRGLARLRSGIIA
                     VSDTGNHRIQLFSPFPHVLLQVWGATDTSGKPIIGTEPRQFHSPTAMVTDHESDSLYI
                     ADQCNKRVQHISADGARVQMIGDTILKRPSRLAIGPRQLLAVVDTALSCVFLFSPHVP
                     LPGILSRIETPRSVTFDQNGTLYIGTGNGRIVVFQPSNSS"
     gene            complement(949..3072)
                     /locus_tag="VN647_01635"
     CDS             complement(949..3072)
                     /locus_tag="VN647_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HWG95757.1"
                     /translation="MPLQDIQLDDRRFDSLMQEAKRRIPRYTREWTDHSESDPGITLL
                     ELFAWLTEMMLYRINRVPEKNYVKFLQLIGIELNPPTPAHADLTFTLTTTSQPIVQVI
                     PQGTRVSLSDQTDGGPVVFETDANLYATNAELKAIQAFDGGQFELLTELNNLEGKFFY
                     PFGKYPQKGSALYLGFSQPFPEGRHQLMMYVFTRDLMEEGKGIGADDLIPPPPVDTVW
                     EYWAGDRLKWQPIRLQQDGTRSLTRSGQVELEVLTGSRFVATKVGLLNREQDPPLNWI
                     RYRIREVLGPGYETAPRLEQVLTNTVTATNAITEREEILGASNGLPNQEFRLAHTPVL
                     PADLASPFEASGEIDLGFRTGSLILEVDEGGGFTPWREVKDFSTSKPEDQHYTLDLPT
                     GTIRFGNGEQGKIPSVLVPPDRIGLPTLAAGSPGGDDPLLTNIRAHSYRWGGGARGNA
                     GMKKITSLETSIPYVDSVTNHRPSEGGKDEETVEEAKQRAPQALRSRSRAVTAEDFEF
                     LATQTPRARIRRAHALPLHHPFYSPQHARTPGPPQTPVPIPGAVTVLVIPDSLPHVPK
                     PVPTEDTCTQVARWLKRHALVTTEVYVAPPKYRQIHIEARVQAKPTANLARLQELLTA
                     KFLAYFHPLTGGSEGLGWPFGEKIYFSEIYRLILTTEDVARVNADDVTIFSDGSRVTE
                     CSDIDLAEDEVVYSSDHKVEVSYNA"
     gene            complement(3072..3467)
                     /locus_tag="VN647_01640"
     CDS             complement(3072..3467)
                     /locus_tag="VN647_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HWG95758.1"
                     /translation="MNDFIGTGFRFPIRINGRGGLSWSSGPERIQDSIWIIITTALGE
                     RVMRPRFGAGVSNFVLNSNSLVVRTQLSDAIKRALVEWEPRIELEAVRVDEVSEQASQ
                     VLIGIDYRLRETNELFNMVYPLYVQEGTI"
     gene            complement(3477..4250)
                     /locus_tag="VN647_01645"
     CDS             complement(3477..4250)
                     /locus_tag="VN647_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95759.1"
                     /translation="MPNIDVTVSSDLKKLFELPPCKDVKLPGPKPVKVTLPTGGSIQA
                     FADISKGIPTDCSMSFNLMVQLAPFLAASDCLLKLLGIIAPLIDVVKGLGPPPDPIKL
                     GGAIPKFIKAAEKLAPCLLVVTGAPLIPFIRDVLALIIKILNCFLGQLKTIMGVMNGL
                     SLQIATAAQDGNSELQSILECARENAATSSEHLMKSIEPIGVLLELLGPILELAGQPS
                     IQLPKVGSQTDMDSLNQVIETMQTVLATLQTIVEALGGE"
     gene            complement(4274..4789)
                     /locus_tag="VN647_01650"
     CDS             complement(4274..4789)
                     /locus_tag="VN647_01650"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HWG95760.1"
                     /translation="MSQINGVVVGLVTNVEDRSSQGRIRVRFPWLAEQHETDWIRIAT
                     TMGGKGRGTFFMPELNDEVLLAFEHGNVRFPYVVGFLWNGKDEPPADHVRLRRIQSVN
                     GHRISFIDATPSGGSKGALVIEDAHGNTIALSNGKITIKSVGTLELNAPLITLQGPGY
                     RRVVTPNSNPI"
     gene            complement(4786..6000)
                     /locus_tag="VN647_01655"
     CDS             complement(4786..6000)
                     /locus_tag="VN647_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95761.1"
                     /translation="MPGIEVIDQPIPINVYEGQDFYVPAFEIKVEGQNAPATDIMSVT
                     YSDDLDQIDSFDLTVNNWDPGAHQNAPQVWHQSGNFKYSDRDTYNPWKDVEVWMGYIR
                     KGQKDLRCMLTGEITTLRPNFPSSGPPTLGVGGLNLLHRFRSQQESKDFFNKTDAEIA
                     QIIVDDIAKELKKKSPRLNLRLDTQNLPNQIRLKHITMHNQFPIVFLRERARALGYEM
                     IMAETSSPNDRTVTLRFAPTQTVKRKTYVLEWGKTLLSFQPTLQTANQVAELTVRGWN
                     PQTKEEIKYVARREDLKDIVKPGELGLSEPATVKKKEIVVDYPIQDQAEAKAVAEDRL
                     RQVCETIVIGKGKTVGLPDLRAGVKILIKGLGKRFSGPQEKPFAYLVTSTTHSIGDGG
                     YTTDFSARMEIL"
     gene            complement(6007..6672)
                     /locus_tag="VN647_01660"
     CDS             complement(6007..6672)
                     /locus_tag="VN647_01660"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWG95762.1"
                     /translation="MGLDKLQITPERGQPIHALFNPERYTVNKAVQFAEIAIPGLDSP
                     VVQFVRGQAEKITMDLFFDTTQYGMVDDVKDVRELTGRVYQLTKVHSETHAPPRCVLL
                     WGKAGKLFSFGTKVSPWCVVESVNEEFMLFSPSGVPLRAKLTVSFREAWTIEEQLRET
                     PRHSSDRTKYRTLKRGETLSYLAFEAYQDPGAWRLIAEANGIENPRLIPPGTRLTIPR
                     KEA"
     gene            complement(6684..6878)
                     /locus_tag="VN647_01665"
     CDS             complement(6684..6878)
                     /locus_tag="VN647_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95763.1"
                     /translation="MDGPDIRIGEVVTDIVVTEGVGTLSADDVKRIVALVVEHLRHEQ
                     ERITQRTRDTAIHNQAYQRS"
     gene            complement(6880..7368)
                     /locus_tag="VN647_01670"
     CDS             complement(6880..7368)
                     /locus_tag="VN647_01670"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWG95764.1"
                     /translation="MASGFRIDPLGVFNFYLTLIDSSNVVGTLITAALNYKVAGFSEC
                     SGLDATVEVFEYKEGGQNGFVHKFPTRASHANITLKHGVIYLYDDLWTWHYDFVQGKG
                     TRKDGLIVLLNEARMPVKVWKFKKGIPMKWVGPSLNAGQSNVAVESLEISHQGLLLEV
                     GA"
     gene            complement(7381..9420)
                     /locus_tag="VN647_01675"
     CDS             complement(7381..9420)
                     /locus_tag="VN647_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HWG95765.1"
                     /translation="MAVQQLDTERRRRTYEAPGIYRRLQVNETTDVRLVRTDVAGFVG
                     FTERGPLATADMDAREAMQTAVRLTSWKEYVSTFGWFLQYSHLAYAVRGFFENGGTTC
                     YVVRTAATKSPLLSKQPATAHYTFPGISAAGSITAATALKSAATRGDVSLQVETTKGF
                     FTNDLVRMNGKGVQTFSVIESIEPPHTVHLSSPVTHLFENGSAIFRYNPALQIAASSM
                     GSWGNRLRLLLIPLEHGHAVTEFSLRITVDRGRDLARPFEEEFYNRLSLHPKSEVFAP
                     RIINKRSHLITIPYPTDSTDQIEDLSDIEIPSSAIPLLVQDDIWKLEAQYLQGGRDGL
                     DDVTPSDLIGGTGDLRGLRLLEEIDEVSILCMPDAVPRFAPRTTIEPPRPPSDPCRPS
                     TTPSTPLAKELPVDETSKSPSWTSDKTTPMYRAMIDQCERLRDRVAILDYPPTRSVPT
                     AAQLLAWRNQFVTRFGAIYYPWFLVPDAANPAGPPRPLPPSGHVAGIYARIDNQFGVQ
                     RPPGNEPFEFVTDVQQEISSLQQEQLNPYGINVIRSFPGRGIRIWGVRSLAKAEADPV
                     WQFIHVRRVMSMIEESVDESMQWAVFEPNDEALRKTVVHSLSVFLEAIWQRGGLKGNM
                     PSEGFYVKCDETNNPQSVIDAGQLVCEIGVAVAAPMEFLVFEIRNSPSGAAVVEL"
     gene            complement(9449..9889)
                     /locus_tag="VN647_01680"
     CDS             complement(9449..9889)
                     /locus_tag="VN647_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWG95766.1"
                     /translation="MADGKRIDPYGQFNFLVEIDGVIKGGFTEASGLTTDTNIIEYRE
                     GNESHGTTRKLPGLMKYNNIVLKRGWTQDKSLWEWRKKVIDGKTKRTSGSIVLMDEAR
                     QPALRWNFREGWPSKWEGPALNGKTSEVALETLEIAHEGLELAS"
     gene            complement(9920..11467)
                     /locus_tag="VN647_01685"
     CDS             complement(9920..11467)
                     /locus_tag="VN647_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HWG95767.1"
                     /translation="MPEYLYPGVYVEEIDTGNKPIEGVSTSTVGFIGLAEKGPLKPTL
                     VTSFGEYTRIFGRHTQQVTKNNVTSQEDRLLTYAVEGFFQNGGKRCFVTRVAHKDTAN
                     PAKSAKPAAKAFGGMQVTAVGPGEWGKRIAVEVAPASSADPDFFKLIVRYWAGTPPTN
                     LIDDPTMVEVFEDLSASPQSGNFYESRVTYVSELIELKQTASGIPSVSTPKLLENGTE
                     GDPIAIDDFKGDDSDPENVTGLAALALVDEVSILCCPDEFYLKADDDRIAKELVSQCE
                     NLKYRFAILQSPRSAKKPDAHFPTAPSKRGYAAYYHPWLWVTRPVVGTSVLIPPGGHI
                     AGIYARSDTDRGVHKDPANEIIRGIDELQLQINAQQQAILNPKGINALRNFRGLGDLV
                     WGGRTTSTDPDWKYINVRRLFIFVEKSIERATQWVVFEPNDEPLWARVRRSVSDFLTR
                     LWMDGMLQGRSKEEAFFVKCDRTTMTQADIDNGKLIMMIGIAPVKPAEFVIFRIGQWT
                     GGSEVTE"
     gene            complement(11486..12184)
                     /locus_tag="VN647_01690"
     CDS             complement(11486..12184)
                     /locus_tag="VN647_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95768.1"
                     /translation="MPVVGLKTVAPDEHQVQLKLAVILSDQFTKHRELRGPVSMVIAG
                     KPQIGPYIPYTKQDEGTFCFYDDLPNGTYTLQIRSDDKPPIYLPADISLSVPQSDSAL
                     DPKWPAYPNLALADRALPLDDPAQPQKYKDQRKLSMLRPTVFYPFPADATLIRGRVLS
                     GNNPVGNATVSHDGGESNYVTEKDGWYVLFVRNLAGLAHQITIRTTKSGFTDNTMTVT
                     AKRGQTVTQDIVLS"
     gene            complement(12186..12770)
                     /locus_tag="VN647_01695"
     CDS             complement(12186..12770)
                     /locus_tag="VN647_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HWG95769.1"
                     /translation="MSEYTVLAEVGESLVQVLWEEIQQDPQVSVLIDNENRISLESPF
                     DLRDNDAVRLSIYLYRVVEDPNTKNRFPVAGNGGTVRKPPLTLDLHYLITPMVGAPRE
                     QQILLGKVMQVLYDRAILEGNDLAGSLAAVNEPLRIILNSVSLEETARVWQAMEMSYR
                     LSVCYMARVAILDSTRERYIQPVLQKNSVYGEQN"
     gene            complement(12796..13659)
                     /locus_tag="VN647_01700"
     CDS             complement(12796..13659)
                     /locus_tag="VN647_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95770.1"
                     /translation="MEAPTQGQQNTTGGSCQPGSNGKADPCVPKDIITKLTARSVELE
                     QEKTRSEKQKQAVDADKAAIESEANKLSKTEEDYKKEVANNEREKQEFQCYLESLSKV
                     VEQTIGAHRRRLDEFIKKNRDELAACKQTVEELKQARDHAKEELETSNKSLESKSKAY
                     NDVAGETITTKLSSVSSLKTQITLENTNNHLCTVYFLLRELELELNSLNIQSTAEFHA
                     SLYRAFCEWVDAKQKVRDAQWKLDQSEAELSAKQTECANKNETFRTDLIEQLNKWDCS
                     PCSDKSTTQSC"
     gene            complement(14054..>15064)
                     /locus_tag="VN647_01705"
     CDS             complement(14054..>15064)
                     /locus_tag="VN647_01705"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWG95771.1"
                     /translation="CRQETSIHYGTLVGRSPCFVKVVTQIPSLAATDATVLVQGETGT
                     GKELFARAIHEQSSRKDRAFVPVNCGALPDQLVENELFGHLKGAYTSAYTPQRGLVAE
                     ADGGTLFLDEVDTLSPSAQVKLLRFLQDRQYRPLGSSKSYYGDVRVIAATNTNLTKQV
                     VDQRFRQDLFYRLNILSLTIPPLRERVQDIPLLASHLLAGHQPRGTAGQFRFSTESIR
                     KLLGYHWPGNVREMEGIVQKASFLATSPILGPETIDLSVEPSHTNNHGHSTMRDEKFA
                     AVRNFEQTYLAKLLRTSQGNVTHAARLAGKDRRAFQRLLQKHHLDRSQFSSGPSPSHT
                     PY"
BASE COUNT         3296 a         3812 c         3964 g         3992 t
ORIGIN      
        1 gggagctgtt gctcggctga aatacaacga ttcgcccgtt ccccgttcca atgtacagcg
       61 taccgttttg atcgaaggtc acactacgag gtgtttcaat acggctgagg atgccgggaa
      121 gcggcacatg cggagaaaag aggaagacgc aggacaacgc ggtatcgacg acagccaaga
      181 gctggcgggg gccgatggcc aagcgcgagg gacgctttag aatcgtgtct ccgatcatct
      241 ggacccgagc cccgtctgca gagatgtgct gaacgcgctt gttgcattga tcagcgatgt
      301 atagagaatc ggattcgtga tccgtgacca tggccgttgg ggaatggaac tgccttggct
      361 ccgttccgat gattggctta ccgcttgtat ccgttgctcc ccatacctga agaagcacat
      421 gggggaatgg tgaaaacagt tgaatgcgat ggttgccggt atcggaaacg gcgataatcc
      481 ctgagcgcag tcttgctaag ccgcgtgggt gtctgaactt tcgaggctcg atgcccgatc
      541 ctccgaaagc cgtaatcgta tcgatgcgac cgcctaagta aggttggctg aagtctcggt
      601 tcgtaagcgt aaccagctct tctgcagatc gaaatcgtat gcgtttcaca cggttgagag
      661 tcgcgtcgat cacgagaatg ctgcttccgc atggccccgc cgcaacggcg cttgggcagc
      721 acaacgatcc cggtttggag cgtgagcgtt tggctttctt acctgttgag ctgccacagg
      781 gctcgcccat gatctttgga tcaaggagcg gacgagcctg gccggtgaga gcggggagca
      841 tcaaagctcc atcgttcgga cggggacgca gcttggaacg cattgattcg gacgcatgcc
      901 agccgttcac cggattgaga aattggtatt gttccgttat ggtcgccatt atgcgttgta
      961 gctcacttcc actttatgat cggaggaata cactacttca tcttcggcaa gatcgatgtc
     1021 cgagcattct gtgactcgac ttccatcaga aaagatcgtg acatcatctg cgtttaccct
     1081 tgcgacatct tctgtagtga ggatgagtcg atagatttcg gagaagtaga tcttttctcc
     1141 gaatggccag ccaagccctt ctgatcctcc ggttaatgga tggaaatagg ccaagaactt
     1201 tgcagtgagc agctcctgga gtcgtgcaag gttggctgtg ggtttagctt gtacccgtgc
     1261 ttcgatgtga atttggcgat acttgggcgg ggcgacatac acctctgtgg tcacaagggc
     1321 atgtcgcttg agccagcgtg ccacctgggt gcacgtatcc tccgtgggta ccggcttggg
     1381 gacatgggga agactgtcag ggataacaag cacagtcacc gcacctggta ttggaactgg
     1441 agtttggggc ggtccaggtg ttctggcatg ttggggactg tagaacggat gatgcaaagg
     1501 aagcgcatgg gcgcgacgga tcctcgcgcg aggggtctgt gttgcgagga actcaaaatc
     1561 ttctgccgtc actgctcgag accggcttcg caacgcttgt ggggcgcgtt gcttagcctc
     1621 ctcgaccgtt tcctcatcct tccctccttc ggagggtcta tggttggtga cactatcgac
     1681 atagggaatc gaggtttcca acgacgtgat cttcttcatc cctgcatttc ctcgcgctcc
     1741 accaccccag cggtacgaat gagcgcgtat gttggtgagg agcggatcat ctcctcccgg
     1801 gcttcctgcc gccagggtgg gcaagccgat acgatcaggt ggtacaagga cggaaggaat
     1861 tttgccctgt tcaccattac caaaccggat tgtgcctgtg ggaaggtcga gtgtgtagtg
     1921 ttggtcttcc ggtttggagg tagagaagtc ctttacctct cgccacggtg taaatccgcc
     1981 tccctcgtcc acttcgagta tcaacgaccc ggtgcgaaag ccaagatcaa tctctccgct
     2041 tgcctcaaat ggggatgcta ggtcagcagg caatacaggg gtatgcgcaa ggcggaactc
     2101 ttgattggga agcccattgc tcgctcccaa tatttcctct ctctcggtta tggcattcgt
     2161 cgccgtgacg gtgttggtca atacctgttc gagccggggg gctgtttcgt atccagggcc
     2221 gagcacttcc cggatgcgat aacgaatcca attcaatggc ggatcttgtt cacggtttag
     2281 taaaccgact ttggttgcca caaatcgtga accagtgagg acttctagtt ccacctgccc
     2341 gctcctggtg aggcttctcg tgccatcctg ttgaagtctg atgggttgcc acttgagtcg
     2401 atcaccagcc caatattccc aaacagtgtc gacaggagga ggtgggatga gatcgtctgc
     2461 gccgatacct tttccttctt ccatcaaatc tcttgtgaag acgtacatca tgagctgatg
     2521 tcggccctcc ggaaagggtt gactgaatcc gagatagagt gctgagcctt tctgtggata
     2581 cttcccaaat ggatagaaga atttcccttc caggttattg agctccgtga gcagctcaaa
     2641 ctgtcctccg tcgaatgcct ggatggcttt gagttccgcg ttggtcgcat agaggttggc
     2701 atccgtttca aacacaaccg ggccgccgtc tgtttgatcc gacagactga cacgggttcc
     2761 ctgtggaatg acctggacga tcggttggct ggttgtcgtc aacgtgaagg ttaggtctgc
     2821 atgggctgga gtcggaggat taagctcgat tccgataagc tgaagaaact tcacgtagtt
     2881 cttttccgga acacgattga tgcgatagag catcatctcc gtgagccagg cgaagagctc
     2941 caagagagta atgccggggt cactttcact atgatctgtc cactcgcgtg tatagcgcgg
     3001 gatgcggcgt ttagcttcct gcatcaagct gtcgaagcgc cgatcgtcga gttggatatc
     3061 ttggaggggc atcagatcgt tccttcttgt acgtacagtg ggtagaccat attgaagagt
     3121 tcgttcgttt ctcgaagccg gtagtcgatc ccaatcaaca cttgactggc ctgttcggaa
     3181 acttcgtcca ctcgcacggc ctccaactcg atgcgtggtt cccattccac cagcgcacgt
     3241 ttgatggcat cagacagttg tgtgcgcacg acaaggctat tcgaattcag cacaaagttg
     3301 gaaacgccgg ctccaaatct cggccgcata acacgttcac caagtgctgt ggttataatg
     3361 atccatatcg aatcttgaat acgctcaggg ccactcgacc aactcagtcc gccgcgaccg
     3421 ttgattcgga tcgggaatcg aaatcctgtg ccaataaaat cattcatgtg ccttgatcac
     3481 tctcctccga gtgcttcgac aatggtttga agggtggcca ataccgtctg catggtttcg
     3541 atgacctgat tgaggctgtc catgtccgtt tgacttccta ctttcggaag ctgaatcgac
     3601 ggttgtccgg ccaattcgag aatgggccct agaagttcga gcaagacccc tataggttct
     3661 atcgacttca tgagatgctc cgacgaagtt gcggcatttt ctctcgcgca ctccaggatg
     3721 ctctgtaatt cactgttgcc gtcttgagct gcggttgcaa tctggagcga caatccgttc
     3781 atgactccca tgatggtctt gagctgaccg aggaaacagt tgaggatctt gatgatcaac
     3841 gcaagcacat ctcgaatgaa tgggatcaac ggtgcaccgg tgacgaccaa gaggcacggg
     3901 gccaattttt ctgcagcttt gatgaacttt ggaatagctc ctcccagctt aatgggatcg
     3961 ggtgggggac caagcccctt cacgacgtcg atcagcggag caatgatccc caacaatttt
     4021 agaagacagt ctgacgcggc aagaaaaggg gccagttgca ccattaagtt gaaagacatc
     4081 gaacaatcgg taggaatgcc tttcgagatg tccgcgaacg cttgaatgct gcctcctgtc
     4141 ggcagagtaa ccttgacggg ttttggccca ggcagtttga catctttgca aggaggcagt
     4201 tcaaaaagct tcttcaggtc tgaggaaacg gtcacgtcaa tgttcggcat aatgtcctat
     4261 ctctatggct caatcagatc gggttactgt tgggagtcac gacacgccgg tacccaggtc
     4321 cttgcaatgt gatgagtggg gcattgagtt ccagggttcc gacgctcttg atcgtaattt
     4381 ttccgtttga cagggcaatc gtattgccgt gggcatcttc gatcaccaaa gcccctttac
     4441 tcccaccgga aggggtggca tcgataaaac tgatccgatg tccattgact gactggattc
     4501 ttctgagcct gacgtgatca gcggggggct cgtccttccc attccataga aaacccacta
     4561 cataggggaa tcgaacgttc ccatgctcaa aggcgagcag gacttcatca ttcagttccg
     4621 gcatgaagaa tgtgccgcgc ccctttcctc ccatcgtcgt tgcgatgcga atccagtcag
     4681 tttcatgctg ttctgctaac caggggaatc gaactcgaat acggccttga cttgaacgat
     4741 cttcgacatt cgttaccaac ccgactacga caccattgat ttgactcata agatttccat
     4801 cctggctgaa aagtctgttg tgtaaccccc atcgccgatg gagtgggtgg tcgatgtcac
     4861 aagataagca aacggtttct cttgaggtcc tgaaaatcgc ttcccaagtc ccttaatcaa
     4921 gatcttgaca ccggcacgta gatcggggag gccgactgtc ttccccttcc caatcacgat
     4981 cgtttcgcag acttgcctca agcgatcttc cgcaacagct ttggcctctg cctgatcctg
     5041 aatgggatag tccaccacga tttccttttt cttcaccgtg gctggttcgc tcaaacctag
     5101 ctctccgggt ttgacaatgt ctttgagatc ttcgcgcctg gccacatatt tgatttcttc
     5161 tttcgtctga ggattccagc cgcgcactgt cagctcagcc acttgattgg ccgtttgcaa
     5221 tgtcggttga aagctcagca gagtcttccc ccactccaag acatacgtct tgcgcttcac
     5281 ggtctgcgtc ggagcaaatc gcagggtgac ggttcgatca tttggactgg acgtttccgc
     5341 catgatcatt tcgtatccca aggcccgggc tcgttcccga aggaacacaa tgggaaactg
     5401 gttgtgcatt gtgatgtgct tgagtctgat ctggtttggg agattttgtg tatctaggcg
     5461 aaggttcaaa cggggcgatt tcttcttcag ttctttcgcg atgtcatcga cgatgatttg
     5521 ggcgatttct gcatccgtct tgttgaagaa atctttggac tcttgctgag atcgaaaacg
     5581 atgtaacaaa ttaagacctc cgacccccaa tgtcggcgga ccggatgaag gaaaattcgg
     5641 tcgaagtgtc gtaatttctc ccgtcaacat gcaccggaga tctttctgcc cctttctgat
     5701 gtaccccatc cacacttcta cgtctttcca ggggttgtag gtatctctgt cgctgtattt
     5761 aaagtttcct gattgatgcc atacttgagg tgcgttctga tgagctcccg ggtcccagtt
     5821 attgacggtc agatcgaatg aatcgatctg gtcgaggtca tccgagtagg tcacgctcat
     5881 gatgtcggtg gctggtgcat tctgcccctc gaccttaatc tcaaacgccg gaacataaaa
     5941 atcctgtccc tcgtatacat tgataggaat aggttgatcg atcacttcga tgccgggcat
     6001 aggctgctac gcttccttcc gtggaattgt gaggcgtgta ccaggaggaa ttagcctcgg
     6061 gttctcgatg ccatttgctt cggcgatcag tctccacgca ccagggtctt gataggcctc
     6121 aaaagccagg taactcagtg tttccccccg cttcagcgtt cggtatttgg tccggtcgct
     6181 gctatgtcgt ggtgtttcgc gaagttgttc ttcgatcgtc catgcctctc ggaatgagac
     6241 ggtgagcttc gcgcggagcg gaactcctga tgggctaaag agcatgaatt cttcattcac
     6301 gctttctacg acacaccagg gtgagacctt tgtgccgaag gaaaacagtt tgccggcttt
     6361 cccccacagc agcacacacc gtggtggggc gtgtgtttcg gagtgcactt tggtgagttg
     6421 atagactcgc ccggtgagtt ctcgaacatc ttttacgtcg tcgaccatac cgtattgagt
     6481 ggtatcaaaa aagagatcca tcgtaatctt ttccgcctgc ccgcgaacga actgcaccac
     6541 aggagaatca aggccgggaa tggcgatttc ggcaaattgg acggctttat tgacggtgta
     6601 tcgttcgggg ttgaacaatg catggatcgg ctggccgcgt tcaggcgtga tctgtaactt
     6661 gtcgagtccc atgcgacttc cttttagctc ctctgatagg cttgattgtg aatcgccgta
     6721 tcacgggtcc gttgcgtgat ccgctcttgc tcatggcgaa ggtgctcgac gaccaaggcc
     6781 acgatgcgct tgacgtcatc agcactcagc gtaccgactc cctctgtgac cacaatgtcg
     6841 gtcacgacct caccgattcg aatatctggt ccgtccattt taagccccca cctcgagcag
     6901 caacccttga tgggaaatct ccaaggattc gacggctaca ttgctctgac cggcattgag
     6961 cgatgggcca acccacttca tcggaatgcc tttcttgaat ttccacactt tgacgggcat
     7021 gcgagcttca ttgagcagaa cgatcaatcc gtcctttcgt gtgccttttc cttgaacaaa
     7081 gtcatagtgc cacgtccaga ggtcgtcata gagatagatc acgccatgtt tcagggtgat
     7141 gttggcatga ctggcgcgcg tgggaaactt gtgaacaaac ccgttttgcc caccttcctt
     7201 gtactcaaac acttccactg tggcgtccaa tccggaacat tctgaaaagc cggcgacctt
     7261 gtaattcagc gcggccgtaa taagcgtccc caccacattt gaggaatcaa tgagggtgag
     7321 gtagaagttg aagacgccta aagggtcgat tctgaatcct gatgccatat gaatcgttcc
     7381 tcaaagttct acgactgcgg caccgctcgg actgttccgg atttcgaata cgagaaattc
     7441 catcggagct gctacggcta cgccgatctc acatacgagc tgcccggcgt cgatcaccga
     7501 ttgcgggtta ttcgtttcat cgcacttgac gtaaaatcct tctgacggca tgttgccctt
     7561 caaacctccg cgttgccaga tggcttctag aaaaaccgac aatgaatgga ccaccgtctt
     7621 tctaagggct tcatcgttcg gctcaaacac tgcccattgc atcgattcgt ccactgattc
     7681 ttcgatcatc gacatgacac gacgcacgtg aatgaattgc cataccgggt ctgcttccgc
     7741 tttggccaat gagcgcacac cccaaatccg tatccctcgt cccgggaacg aacggatgac
     7801 gttgatgccg taaggattca actgttcctg ctggagggag gagatctctt gttggacatc
     7861 ggtgacgaac tcaaatggct cattgccagg tggacgttgg acgccgaact ggttatcgat
     7921 tcgggcatag atgccggcga catgccccga tggtggaagg ggtcgagggg ggcctgccgg
     7981 attggcggca tcgggaacaa ggaaccaggg ataatagatg gctccaaagc gggtgacgaa
     8041 ctggttgcgc caagcgagca gttgtgccgc cgtcggcact gatctggtcg gcgggtagtc
     8101 gagtattgcc actcgatctc tcaaccgctc acactggtcg atcatcgcac gatacatcgg
     8161 cgtcgtcttg tcagatgtcc aagacggtga cttcgatgtt tcatcaactg gtagttcctt
     8221 ggctagtgga gttgatggtg ttgtgctagg acggcacggg tcactaggtg gccgtggagg
     8281 ttctatggtc gtcctaggag caaaacgtgg cactgcatcg ggcatgcaga ggatagatac
     8341 ttcatcaatc tcctcgagca atcgaagtcc tcgaagatcg ccggtgcctc caatgagatc
     8401 agatggagtc acatcgtcca gaccgtctcg ccctccctgg aggtattgag cctccagctt
     8461 ccagatatca tcctgaacca agagaggtat ggcagatgat gggatctcaa tgtcggacag
     8521 gtcctcaatt tggtcggtac tatcggtggg atacgggatt gtgatgaggt gagatcgttt
     8581 attgatgata cgaggggcaa agacttcact ttttgggtgt agtgatagcc tgttgtagaa
     8641 ttcttcttca aagggtctgg caagatccct ccctcgatcc acagtgattc ggagggagaa
     8701 ttctgtgaca gcatgaccat gttcaagagg gatgaggagc agccgaaggc ggtttcccca
     8761 cgaccccatg ctactcgctg caatttgtaa tgctgggtta tatcgaaaga tggcagagcc
     8821 attttcaaat aaatgcgtta ctggggaaga gagatggact gtgtgaggag gctcaatcga
     8881 ttcaatgaca gagaaggtct ggactccttt cccgttcata cggactagat catttgtaaa
     8941 gaatccttta gtggtctcga cctgcagaga cacatctcct ctggtggctg ctgatttgag
     9001 tgctgtagca gcagtaatgg aaccggccgc agatattcct ggaaacgtat agtgagcagt
     9061 tgctggttgt ttggagagaa gcggtgactt cgttgccgca gtccgcacga cgtagcaggt
     9121 cgtgcctcca ttttcgaaaa atcctcgtac ggcatatgct aaatggctgt attgaaggaa
     9181 ccaaccgaac gtcgaaacat attctttcca gctcgtcagt cgcacggcgg tctgcatggc
     9241 ctcacgtgca tccatgtccg cggtggcgag aggccctcgt tctgtaaagc ccacgaaacc
     9301 tgcgacgtct gtccgaacaa gccgaacgtc tgttgtctcg ttcacttgta ggcgccggta
     9361 gatacccggc gcctcatagg tcctccggcg acgctctgta tcgagttgct gaacagccat
     9421 gacgcctctt agcctcttac tgcaactgtc acgatgcgag ttcgagacct tcgtgtgcga
     9481 tctccagcgt ttccaatgcc acctcgctgg tctttccgtt cagggccggt ccttcccatt
     9541 tgctgggcca gccttcccga aaattccatc gcaatgccgg ttgacgcgct tcgtccatta
     9601 gcacaatgga cccactagtt cgtttggtct tcccatcgat cactttcttg cgccactccc
     9661 atagcgattt atcctgcgtc caacctcgtt tgagtacaat gttgttgtat ttcattagtc
     9721 cgggaagctt ccttgttgtg ccatgggact cgttgccttc gcgatactca atgatgttgg
     9781 tgtccgtcgt gaggccactc gcttctgtaa atccaccctt gatgaccccg tctatttcaa
     9841 cgagaaagtt aaattgccca tatggatcga tgcgtttgcc atcagccatg gagcactcct
     9901 ttcgccttac aatatctgct tactcggtta cttctgatcc accggtccac tggccgatcc
     9961 taaagatcac aaattcggcg ggttttactg gggcaatgcc gatcatcatg atgagcttgc
    10021 cgttatcgat atcggcttgg gtcatcgtcg tgcggtcaca cttcacgaag aacgcttctt
    10081 ctttacttcg accctgaagc atcccgtcca tccagagccg cgtgagaaaa tcactcacac
    10141 tcctccgcac tcgagcccag agtggttcgt cgttgggttc gaagacgacc cactgcgtgg
    10201 ctctttctat tgatttctca acaaaaatga aaaggcgtcg gacgttgata tatttccagt
    10261 ccgggtcggt gctggttgtt cggccacccc aaaccaagtc acctagacct ctaaaatttc
    10321 gcagcgcatt tatccccttt gggttgagga tggcctgttg ctgtgcattg atctgaagct
    10381 gaagttcatc aatccctcta atgatttcat tggccgggtc tttatgaaca ccgcgatcgg
    10441 tatcgctgcg tgcgtagatt ccggcaatat gcccccctgg aggaattaag acggaggtgc
    10501 caactacggg ccttgtgacc cataaccagg ggtggtaata ggccgcataa cctctttttg
    10561 aaggggcagt tggaaagtgg gcatccggct tctttgcaga tctcggcgat tgcagaattg
    10621 caaatcgata tttaagattc tcacactggc taaccagttc cttcgctatt ctgtcatcat
    10681 cagccttgag atagaattcg tccgggcagc ataaaataga tacttcatct actagtgcta
    10741 gagctgccag ccctgtcaca ttttcagggt cagaatcatc tcccttaaag tcgtcgatgg
    10801 cgattggatc gccttccgtt ccattctcga gcaatttcgg ggtcgagacg gatggaattc
    10861 cgctcgcagt ttgcttaagc tcaatgagct ccgacacata ggtgacccga ctttcataga
    10921 agttccctga ttgcggagat gctgatagat cctcaaaaac ttcgaccatc gtgggatcgt
    10981 cgataagatt tgtgggaggt gtaccagccc aatatcttac aattaacttg aagaagtcgg
    11041 ggtctgcgct gctcgcaggg gcaacctcta cggcaatgcg tttcccccac tcaccaggac
    11101 ccactgcagt gacttgcatt ccaccaaagg cttttgctgc tggttttgct gacttcgctg
    11161 gattggcagt atccttatgt gctactcggg tgacgaaaca gcgcttcccc ccgttttgaa
    11221 agaagccttc aacggcatag gttaagagac ggtcttcttg cgaggtgaca ttgttctttg
    11281 taacttgctg agtatgccta ccaaatattc gcgtatattc cccaaagctt gtaacgaggg
    11341 tcggctttaa tggtcccttc tccgctaacc ctataaatcc aactgtgctt gtagagacgc
    11401 cttcaattgg cttattcccc gtgtcaattt cctcgacgta aacacctggg tacagatact
    11461 cgggcatgac tgcctccttt acgatttagg aaagaacaat gtcttgtgta acggtttgtc
    11521 ctcgctttgc tgtcacagtc atcgtgttat ctgtaaaacc ggatttggtt gtcctgattg
    11581 ttatttggtg tgctagccct gcgaggttgc gtacaaaaag aacgtaccaa ccgtctttct
    11641 cagtcacata gttgctctct ccaccatcat gagataccgt tgcgttacca acggggttat
    11701 ttccagacaa cacgcgccct cggattagag tggcatcagc cgggaatgga tagaaaacag
    11761 ttggtctgag cattgagagt ttccgttggt ccttgtattt ctgaggttga gcagggtcat
    11821 ccaacggaag agctcgatct gctagggcaa gattcggata ggccggccat tttggatcaa
    11881 gagcactgtc gctctgtgga actgatagtg aaatatctgc ggggagatag attggtggct
    11941 tatcatcact tcgaatttga agggtgtacg ttccgttggg aaggtcatcg taaaagcaga
    12001 aagttccttc gtcttgtttt gtataaggga tataaggacc gatctgtggt ttgcccgcta
    12061 taaccatgct gactggtccc ctgagttctc ggtgcttagt gaactgatca gacagaatta
    12121 cggccaactt cagttggact tgatgttcgt ctggggcgac cgttttgagt ccaacaaccg
    12181 gcatcctaat tctgctcccc gtatacagag ttcttttgaa ggaccggctg tatgtatcgt
    12241 tctcgagtgg aatccagtat ggcgacgcgc gccatgtaac agacggaaag gcggtaactc
    12301 atttccatgg cttgccagac ccgtgcggtc tcctccagcg agaccgagtt aaggatgatg
    12361 cgaagcggtt cattgaccgc agcaagcgac ccggcgaggt cgttcccctc aagaatggcg
    12421 cggtcatata acacttgcat cactttcccc agtagaatct gctgttcacg cggagcgcct
    12481 accatcggtg tgatgagata atggagatcc agtgtcaacg gaggctttcg aacagtgcct
    12541 ccatttccag ctaccgggaa acggttcttg gtgttggggt cctcgacgac tcgatataga
    12601 tagatggaaa ggcgaacggc gtcgttgtcg cggaggtcaa acggtgattc caatgatata
    12661 cggttctcat tgtcaatgag gacactcacc tgcgggtcct gttggatctc ctcccacagg
    12721 acttgcacca gtgactcccc gacttctgca agaaccgtat attcgctcac gactgtatgt
    12781 ggctcctatg ccgatctaac aagactgtgt ggttgatttg tctgagcagg gagaacaatc
    12841 ccatttattc agttgctcaa ttaaatccgt tcggaaagtc tcgttcttgt tcgcacactc
    12901 cgtttgcttc gcggacaatt cggcctcact ctggtcaagc ttccattgtg cgtctcgcac
    12961 tttctgcttc gcgtcaaccc attcacaaaa tgctctatag agagaggcgt ggaattctgc
    13021 ggtgctctgt atgtttaggc tattcaattc aagctccagc tctcggagca agaagtagac
    13081 agtacagaga tgattgttcg tattttctag ggtgatctga gttttgagcg aggatacact
    13141 gcttagctta gtcgttattg tctcaccggc aacgtcgttg taggcttttg atttggactc
    13201 aaggctcttg tttgaagttt ccagctcttc ttttgcatgg tctcgggctt gtttgagttc
    13261 ctctaccgtc tgtttgcagg cagctaattc atccctgttt tttttaatga attcatcaag
    13321 acgtctacgg tgagcgccaa tggtctgttc aaccacttta cttagtgatt ctaggtaaca
    13381 ttggaattcc tgtttctctc gctcattatt cgctacctcc ttcttgtagt cctcttctgt
    13441 tttggaaagc ttgtttgctt ctgattcaat tgcagcctta tcagcgtcca ccgcttgctt
    13501 ctgtttttca gaacgagtct tttcctgctc gagttccaca gacctcgcgg tgagtttcgt
    13561 aatgatatct ttgggaacac atggatcggc cttcccgttg cttcccggtt gacaggatcc
    13621 gcctgtggtg ttctgctgtc cttgagtagg tgcttccata gctgactcct tcatcctacg
    13681 gtgttgtgct gcattctgtt gtgggtggag agaactgcat atgccgctac tgagcaatga
    13741 gaatgccaac tgcagcagaa gtaaaagccc aaagattttg cagtgcatag tggtggaaag
    13801 attgtgaagt gtggtagtgg aggccgcagt actcttgggc ataagagatc tatgcccatg
    13861 aattgtttct gaaacgtggg actgcggctc ctctatgttc actgcggtga atttgtcgca
    13921 gtcggtgagt tcgatcggaa attgagaaag aactacaagg ggaggtctga gtcacagctt
    13981 tgaactgaaa ccagtcagat ctccagcgct gcgagagctg gctcagtaaa aagcaggatt
    14041 attcaggctt tccctagtac ggtgtatggg aaggtgaggg tccggaggag aactggcttc
    14101 ggtcgaggtg atgtttctga agtaatcgct gaaatgcccg tcggtctttt cctgcaagac
    14161 gcgcggcatg cgtgacattc ccttgcgatg tcctgagaag ctttgcaagg taagtttgtt
    14221 caaagttccg caccgctgcg aatttctcgt cccgcatggt cgagtggcca tggttatttg
    14281 tgtgagaagg ctccacggat aggtcaatgg tttcaggtcc gagtatagga ctcgttgcta
    14341 gaaacgatgc tttttggacg atgccttcca tttctcgaac attgccaggc cagtgatagc
    14401 cgagcaactt ccgtatggac tcggttgaga atctaaattg tcctgcggtt cctcttggct
    14461 ggtgtcctgc caaaaggtgg ctggcgagca gtggtatatc ctgaactcgt tctctcaaag
    14521 gggggatggt caaagagaga atattaagtc gatagaagag gtcttggcga aaacgttggt
    14581 cgacgacttg tttcgtaaga ttggtatttg tggcggcaat cacgcgcacg tcgccataat
    14641 aactttttga tgatcctaga ggtctgtact gtctatcctg caagaaccgg aggagcttta
    14701 cttgtgcaga aggagaaagg gtgtcaactt cgtcaagaaa cagagttcca ccgtcagctt
    14761 cggccacaag cccgcgctgg ggggtatacg cacttgtgta tgctcctttg agatgcccaa
    14821 acaattcatt ttcgactaat tgatcaggta gagctccgca attcactgga acaaacgcgc
    14881 gatccttacg gctactctgc tcatgaatcg cacgtgcaaa taactctttt ccagttccag
    14941 tctctccttg aaccaagacg gtcgcatcgg tcgccgccag tgatggaatt tgtgttacta
    15001 ctttaacaaa acaggggctt cggcccacaa gcgttccata gtggatagat gtttcctgtc
    15061 ggca
//
LOCUS       DATNDD010000076         3832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3540784, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000076 DATNDD010000000
VERSION     DATNDD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540784"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /gene="serS"
                     /locus_tag="VN647_01710"
     CDS             complement(<1..921)
                     /gene="serS"
                     /locus_tag="VN647_01710"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWG95772.1"
                     /translation="MLDSTRLHEERPVYDLKHLRENLDHIRTSLGRRGNDVPWADIQK
                     LSEERRAVTTQVEQFRHELNKGSEEVARLRRAKEPAEAAMAAMKQLGDRIKDAEGTLR
                     RVEEALTDIALRLPNLPHTSVPIGVDASENVEVRQWGSKPSFSSPPRPHWEVGENLGI
                     LDFDRAAKIAGARFSVMTGAGARLERALINYMLDLHTTQHGYREVIPPFMVNRPSMTG
                     TGQLPKFEEDLFRMKDEDYFLIPTAEVPVTNLHREEILGVDRLPLRYTAYTPCFRREA
                     GSYGKDTRGLIRLHQFNKVELVAFTTPDRSY"
     gene            1026..2039
                     /locus_tag="VN647_01715"
     CDS             1026..2039
                     /locus_tag="VN647_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWG95773.1"
                     /translation="MRQISNPPNPFESQHRDLLEPADTAKLMLYADESREILSRNDSP
                     DLPFRWSVNPYRGCFHACAYCYARPSHEYWGFGAGTDFESKIVVKKDAPQLLRRAFDK
                     PSWHGELIVFSGNTDCYQPIEAEYGLTRACLEVCADYRNPVGIITKGALILRDLDVLV
                     RLHRETSVLVYFSIPFSSDDVARQVEPHVPSIAKRFSAMKAVSEAGIPAGISIAPVIP
                     GLNEDDIPELLDRAYGAGARTAAYSLLRLSGSLEPVFLERMREAFPERIGKITNRLRE
                     VRGGALTESRFFKRRAGQGPYWELIGQLFALAKRRAGFPEDDMRSTPQTFRRPGAEQV
                     SLF"
     gene            2123..2422
                     /locus_tag="VN647_01720"
     CDS             2123..2422
                     /locus_tag="VN647_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWG95774.1"
                     /translation="MAEVKARLDRGERFHFIDVREDHEFAKDHVRGARHLGKGIIERD
                     IESVVPDKQDSVVLYCGGGYRSALAADALQQMGYGNVISMDGGIRAWREAGYPLE"
     gene            complement(2436..2984)
                     /locus_tag="VN647_01725"
     CDS             complement(2436..2984)
                     /locus_tag="VN647_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWG95775.1"
                     /translation="MHIPALRTLFLAALLSPLVPFVATAIQAAEFKMGVVDPQSVLEK
                     SKAGKRALEGLKEYVSTRQKLLAKDEEDLRNNEKQLKEQAPKWTDTEKKDKEGQFRTK
                     VQDFQKRAQEFNMELQKKQKELVDEYMKKISTATQAVAEKGGVSIVVDKGSEQTVKIV
                     IYNKDTIDLTEQVIKEFDRTNK"
     gene            complement(3107..3490)
                     /locus_tag="VN647_01730"
     CDS             complement(3107..3490)
                     /locus_tag="VN647_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95776.1"
                     /translation="MPSFNGATNALLVELALPEFTESQRSQLKGRVVELFRTHRSSNG
                     SGDAALTELNQTPRIFQLNMLAIAMKDLGYPLPFKKEKIQKIRDPFDPSHADEYAMRA
                     VARRLKWQYGVEVWIAEESITFDSW"
     gene            complement(3679..>3832)
                     /locus_tag="VN647_01735"
     CDS             complement(3679..>3832)
                     /locus_tag="VN647_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWG95777.1"
                     /translation="YDEDLYKARHLIENFFARLKQFRAIATRYDKRAANFLGAIYLAA
                     SLTWLN"
BASE COUNT          801 a          993 c         1107 g          931 t
ORIGIN      
        1 gtacgaacga tccggtgtcg taaatgccac gagttccact ttgttgaatt ggtgaagccg
       61 gatgagtcct ctcgtgtctt tcccatacga ccctgcctct cgtctgaagc aaggagtgta
      121 ggccgtatag cggagtggta agcggtctac acccaggatc tcctcgcgat gcaggttcgt
      181 caccggcact tcggcagtcg gaatcaggaa gtaatcttcg tccttcattc ggaagagatc
      241 ttcttcgaac ttcggaagct gacccgtccc agtcatcgaa ggacgattca ccatgaaagg
      301 aggaatcacc tctcgatatc catgctgagt ggtgtgcaga tcaagcatat aattgatcag
      361 cgctcgttcc agcctcgcac ctgccccggt catgacggaa aatctggctc ctgcgatctt
      421 cgcggctcga tcgaaatcca agattccgag gttctcgccg acttcccagt gaggtctggg
      481 ggggcttgaa aaagatggtt tggaacccca ctggcggacc tcgacatttt ctgatgcatc
      541 taccccaatc gggactgaag tatgggggag attggggagg cgaagcgcga tatcggtgag
      601 cgcttcctcg acccttcgaa gggttccctc cgcgtcctta atgcgatccc ccaactgctt
      661 catcgccgcc atggctgctt cagccggctc tttggctcgg cgcaatcgag ccacctcttc
      721 cgagccctta ttgagttcgt gcctgaactg ttcgacctgc gtcgtaacgg ctcgccgttc
      781 ttcgctcaat ttttggatat cagcccacgg gacgtcgttc ccacgtcgtc cgagagaggt
      841 ccgaatgtga tcgaggtttt cgcggagatg cttcagatcg tacacaggtc gctcttcatg
      901 aaggcgggtg gaatctagca cctgatttcc agagagtcaa cgaaatcacc agcctccccc
      961 atcatgacca tacgacctgg tatagaacat tgacatcaat cgggcatgag ggacaggata
     1021 ggcttatgcg ccaaatctcc aatcctccaa acccatttga gtcgcaacat cgggacctgc
     1081 ttgaaccagc cgacacagcc aaactgatgc tctacgcgga cgagagtcga gagatcttga
     1141 gccgtaacga cagcccggat ttgccgtttc gatggagcgt gaatccctat cggggttgtt
     1201 ttcatgcctg tgcctattgt tacgcgcgtc cttcgcatga gtattgggga ttcggcgccg
     1261 gaaccgattt cgagagcaag atcgtggtga agaaggatgc gccgcaactg ctgcggcgtg
     1321 ccttcgacaa gccgtcatgg catggagagc tgatcgtctt ttccggaaac acggactgtt
     1381 accagccaat cgaggcagag tacggattga cgcgtgcctg tttggaggtc tgcgcggact
     1441 accggaaccc cgtcggtatc atcacaaaag gtgcattgat tcttcgtgat ctggatgtgt
     1501 tggttcggtt gcaccgagag acttcggtgc tggtgtattt cagtattccc ttctcctcgg
     1561 acgatgtggc tcgccaggtg gagccgcatg tgccgtcgat cgccaagcgg ttctccgcca
     1621 tgaaggccgt ttccgaggcc ggtattccgg ccggtatttc gatcgcaccg gtcattcccg
     1681 gcttgaacga agatgatatt cctgaattat tggatcgcgc gtatggtgcc ggagcccgta
     1741 ctgccgccta tagcctgtta agactgtcgg gcagcctgga accggtgttc ttggagcgga
     1801 tgcgagaggc gtttccggaa cggatcggaa aaatcaccaa tcggctccgt gaggtcaggg
     1861 gcggagcatt gacggaaagt cgatttttca agcgacgggc aggacaggga ccttattggg
     1921 aactgatcgg gcaattattc gctctagcca aacggagagc ggggttccca gaagatgaca
     1981 tgagatcgac ccctcagacg tttcgtcggc cgggcgctga acaggtgtcg ctgttttgaa
     2041 gggaggggag gagtcatgaa acataatcca gggttcttgc accttgtcga acaggcgaaa
     2101 cgaacgtgtc aaggaatgta gcgtggccga ggtgaaggct cgtctcgacc gtggcgagcg
     2161 gttccatttc atcgatgttc gggaggatca tgaattcgcc aaggaccatg tgcgaggcgc
     2221 tcggcacctt gggaagggga tcattgagcg ggatatcgag tcggtggttc ctgataagca
     2281 ggactcagtc gtgctttatt gtggcggggg gtatcgatcg gcgctggctg ccgatgcctt
     2341 gcaacagatg gggtacggga atgtgatctc gatggatgga ggaattcgcg cgtggcggga
     2401 ggctggatac ccgctggaat aggcgatgcc ccacgttact tgttcgttcg atcgaactcc
     2461 ttgatcacct gctcggtgag atcgatcgta tctttgttgt agatcacgat cttgacggtc
     2521 tgctcgcttc ctttgtcgac gacgattgaa acccctcctt tttcggcgac cgcttgtgtc
     2581 gcggtggaga ttttcttcat gtactcgtcg accaactcct tttgcttttt ctggagttcc
     2641 atgttgaatt cctgcgcgcg cttctggaag tcctggacct tcgtccggaa ttgcccctcc
     2701 ttgtccttct tttccgtgtc ggtccacttg ggggcttgtt ccttcaattg cttctcatta
     2761 ttgcgaaggt cttcctcatc tttggcaaga agcttctgtc tggtcgagac atattccttt
     2821 agcccttcga gcgctcgctt ccctgccttg gatttttcta agacggactg cgggtccacc
     2881 acccccatct taaattcagc cgcctggatg gctgtcgcca cgaatggaac aagcggagag
     2941 agtagcgccg caaggaacag cgttcgcaaa gcaggtatat gcatattgtc tcttgtgcct
     3001 cctgacctga atgaaggaac ggcggcagaa taaccgcgca ccgcgggtct gtcaagcgat
     3061 cagccttggt aagaatctca attggctcga cagggcaatc agatgactac cacgaatcaa
     3121 aagtgatcga ctcttccgcg atccagacct cgacgccata ctgccacttc aggcgccgcg
     3181 cgactgctct catggcgtac tcgtccgcat ggctcggatc gaatggatcc cggattttct
     3241 gtatcttttc ttttttgaac ggaagggggt acccaaggtc cttcatggcg atcgccagca
     3301 tgttcagctg aaagatgcgt ggcgtttgat tcagctctgt caatgcagcg tcccccgatc
     3361 cattggaaga gcgatgcgtt cggaacaatt caacaacccg gcctttcaat tgagagcgtt
     3421 gagattcggt gaactcgggg agggctagct cgaccaagag tgcgttggtg gcaccattga
     3481 acgatggcat gtcgagaaat ctgcttaaaa atcccatcgg aatcctcgct gctagatcgt
     3541 gaagccgtga tgcggcagcc gttgcttctg gcaaagcggc tagagattcg ggggagtgta
     3601 ctgaaacggg cggtgatttt ccagactgca aaaagagggg gatgatgaat gagagccggt
     3661 cgaaattagg gcgtgtcatc aattaagcca ggtgagtgaa gcggcgagat agatggcgcc
     3721 gaggaagttg gcggcgcgtt tgtcgtagcg agtcgcaatg gcgcggaact gcttgagcct
     3781 tgcaaagaag ttttcgatca gatgccgcgc cttgtacagg tcttcatcat ag
//
LOCUS       DATNDD010000077         5124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1782459, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000077 DATNDD010000000
VERSION     DATNDD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782459"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1254)
                     /locus_tag="VN647_01740"
     CDS             complement(235..1254)
                     /locus_tag="VN647_01740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWG95778.1"
                     /translation="MNQTNIQDGDAYTVVVFRGSTAKPIRFSFSKKLLRRLLVCVGLL
                     ILADLLVVSHYVIRTGEVWELAAFRTEAMSAREQTAAFSTAVDDLKKRLGAMNEVNQR
                     LRVMLGIEVPKTGDMANGRGGEEVPIPEERGMIPGENEPKPSSGISRQISDGNQEYSS
                     AVGLDSSRDNLQAIASVKESLERLSKQATVQERILDELSQAAEQRSSRWASTPSIWPV
                     KGWVTSGFGPRISPFTEKPAWHDGLDIGAAPNTPVHAPAQGRITSIGYDPKLGNIVRV
                     DHGFGVETLYGHLAKALVKEGQRVERGDVIALVGSSGLSTGPHLHYMVKLNGQALDPT
                     KYILE"
     gene            complement(1372..1701)
                     /gene="cutA"
                     /locus_tag="VN647_01745"
     CDS             complement(1372..1701)
                     /gene="cutA"
                     /locus_tag="VN647_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HWG95779.1"
                     /translation="MGVEPIHVVIVMVTTASQGEAIKIAEEVVQSRLAACASMIPAVH
                     STYWWEGKMINDQESLLLIKTTSDKFNSLEETIRKIHSYKVPEIIAIPVSNGFPPYLE
                     WVLQETS"
     gene            complement(1988..3283)
                     /locus_tag="VN647_01750"
     CDS             complement(1988..3283)
                     /locus_tag="VN647_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWG95780.1"
                     /translation="MVEVPSISMDSTRSGDMRRMADLARQYLAGMNAEVQVVETGGYP
                     IVSGGWITGPECPTVTIYNHLDVQPAQEPEWRQAPFVFKNENGIYRGRGATDDKGPAL
                     TAMFGARYAIEQGWPINIRFLWELEEEIGSPHFGVGLKNRSAIPRPDSVVVSDTIWIA
                     KGRPAAPYGLRGLLGARLCLHTGSKDAHSGVTGGAARNPLAELMEVANTCVDARTGRV
                     KISGFYDDVIEPTKREIKNFLTSGFQIRRFKEAYGFRSLRVQDPAEVMRRIWASPTFE
                     IHGLSGGYHGQGIKTVVPGHAELKISMRLVPNQVPEKVFALLKKHVEKVNPDVKVEKA
                     GTLHPFKGSFAGPYVDCAKRAIKAGFGKDPAFVREGGSIGAVVTMQKTWKVPILFIGL
                     SLPEHGYHAPNEYYDWGQASGGMKTFAHYFWELANMRKT"
     gene            3468..3935
                     /locus_tag="VN647_01755"
     CDS             3468..3935
                     /locus_tag="VN647_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95781.1"
                     /translation="MTTSTRIVAPLFVVLLTLIGANTPPLVWAEESTSIQQILEEPQV
                     YHLRHVTLQGTVRDVQPLAPYTVPNGDYCYGAYLFRLEEDAATIPVAVLGICGRPIIR
                     DPEVEDGDRVEVSATIQAPSHGGYYLSFKGLKVVTEQEGVVQAVADRILPIVE"
     gene            4056..4796
                     /locus_tag="VN647_01760"
     CDS             4056..4796
                     /locus_tag="VN647_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95782.1"
                     /translation="METRGLRLRIGLALIVTVSAGCASSQGFDRAAMIEALHVDSSTE
                     QDGQPIANQVSRFSPPFRLGVFFVDHNIPTGQSIRKMEWLSEDRDQLLRELAPLQDEH
                     ILTDTFVLMDATLRGEDIRAIRQAGARFGADLVLIIDGVAAIDRYNNRSAWWYPTLIG
                     AYLARGTESHALVMTTGILWAVRFEWHAPLQRTEEAAKLVGPAAFVEDIAALQEARKR
                     AIEAISKRIVDQLRFLAVGLPREKPNSR"
     gene            complement(4788..>5124)
                     /locus_tag="VN647_01765"
     CDS             complement(4788..>5124)
                     /locus_tag="VN647_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWG95783.1"
                     /translation="NPRTFVMGGNMVVRWHITGPWSVALRPEFYWDRNGRWTGAEQFV
                     KAITSTVEYKLPYKWMNTVTRIEHRYDESTGVGGGFFKDGGLRPGEISLTPGQHLLLL
                     GVLLSFDSP"
BASE COUNT         1195 a         1438 c         1267 g         1224 t
ORIGIN      
        1 cccaagctgg gaaccgattt cgacaatggg acgaagggta acagattggg cgatttcgag
       61 gtcggtcaaa aggctctcat cttgttttgc ctaaatcgca aagggtcgaa gtatagcata
      121 ggcagtcggc aatgattgga agctagctaa cgaaataggg ctgagaataa ttggactggc
      181 caggaggcag caggaacagg tcggtcgaat ctcagatacg tactgaaccc atcgctactc
      241 taagatgtac ttggttggat cgagtgcctg gccgttcaat ttcaccatgt aatgcaggtg
      301 gggccccgtg gacagacccg aactcccgac aagcgcaatg acatcgccac gctcgactct
      361 ctggccttcc ttcactaagg ccttcgcaag gtgtccgtag agagtttcga ccccgaatcc
      421 atgatctaca cggactatgt tcccaagctt aggatcatat cctatggatg taatccttcc
      481 ttgggccgga gcatggaccg gagtgtttgg cgcggccccg atatccaatc catcatgcca
      541 ggcaggcttc tccgtgaaag gagagattct cgggccaaac cccgacgtaa cccacccttt
      601 caccggccaa atcgagggtg tcgaggccca acgagatgag cgctgttcgg ctgcttgtga
      661 taattcgtca agaatgcgtt cctgcaccgt tgcttgcttt gaaagccgtt ccaagctctc
      721 tttgaccgat gcaatagcct ggaggttatc cctagatgaa tctaggccaa cggcggaaga
      781 atattcctga ttaccgtctg atatctgccg cgagatacca gatgaaggtt ttggttcgtt
      841 ttcacccggt atcatgcctc tttcttcagg aatcggcacc tcttctccac ctctgccgtt
      901 tgccatgtcc cccgtcttgg gaacttcaat acccaacatg actcgaagtc gttgattgac
      961 ctcattcatt gcaccaagac gctttttcag gtcatccacg gcggttgaga atgccgcagt
     1021 ctgctcccgt gcactcatgg cttcagtgcg gaaagccgcc agctcccaca cctctcccgt
     1081 tctgatgaca tagtgtgaca ctacgagaag atcggcaagg atgagcaacc ccacacagac
     1141 cagcaatcgg cgaaggagtt tcttcgaaaa gctgaagcga ataggctttg cagtggatcc
     1201 tctgaacacg acaacggtat aggcatcgcc gtcttgaata tttgtctgat tcatatcctc
     1261 gtctcccccc tgcctattat acctgcctca caaattaccg atttggtccc ctattggtca
     1321 acctaaaaaa tagggggttt ggcgaggcgg tttcaaccac atccacatca tttaggatgt
     1381 ttcctggaga acccactcaa gataaggtgg gaatccatta ctcaccggaa ttgctattat
     1441 ttccggtact ttgtacgagt ggatcttccg tatggtttct tcgagggaat tgaatttatc
     1501 agaggttgtc ttgatcagca gtagcgattc ctggtcgttt atcatttttc cttcccacca
     1561 gtaggttgag tgcacagcgg gaatcattga tgcacaagca gcgaggcgag actgtaccac
     1621 ttcctcagca atcttaatcg cttcaccttg gcttgctgtt gtgaccatga caatcactac
     1681 atggatcggt tcaaccccca tggatggcca gaatacccaa gcgcctctct acaatcaacc
     1741 taatcatttc aacggcgcaa gcactcattc cccggtttat actgagcaac atccatgcct
     1801 ggccaataaa gactagatgt tataattatt atgctttaca ataacttacg accatcattt
     1861 acttgcggat atgcctctag gacgattgaa ctgcatggca aacgtggcgg tcaaacaatt
     1921 tttgggacac tatgttcaaa actgatcttt cgtcattggg ttgtcaaaaa aatagccaat
     1981 accgatccta tgtcttcctc atattggcta actcccagaa gtaatgagcg aatgtcttca
     2041 ttcctcctga ggcctggccc cagtcgtaat attcgttggg agcgtgataa ccatgctcgg
     2101 gcaggctcaa acctatgaaa aggattggta ctttccaggt tttctgcatc gtgaccaccg
     2161 caccgattga tcccccttct cgcacaaatg cgggatcttt gccaaaccct gccttaatgg
     2221 cgcgtttcgc acaatccacg tatggccctg cgaagctgcc cttgaatgga tggagcgtgc
     2281 ctgccttctc caccttcaca tccggattca ccttctccac atgcttcttc aacagcgcaa
     2341 acaccttctc cggtacctgg tttgggacaa ggcgcatact gatcttgagc tccgcatggc
     2401 cgggcacaac cgttttgata ccttgtccgt gatatcctcc gctgagacca tgaatttcga
     2461 aggtcggaga tgcccagatc cgtcgcatga cttctgccgg gtcttgcact cgaagagagc
     2521 gaaacccgta ggcctctttg aatcgtctga tttgaaagcc ggacgtaagg aaattcttga
     2581 tctctctctt cgtgggctca atcacgtcgt cgtagaaacc cgatatcttt accctgccgg
     2641 tcctggcatc aacacaagtg ttcgccacct ccatcaactc ggccagcggg ttacgagccg
     2701 ctcctcccgt cacacccgag tgtgcatctt tggacccggt atgtaggcag agtcgtgcgc
     2761 cgagcaggcc gcgcaagccg tatggcgcag caggccgccc cttggcaatc cagatcgtat
     2821 ccgacacaac cacggaatcc ggtcgtggaa tagcgctccg gttcttaagc ccgacgccaa
     2881 aatgcgggct cccgatctct tcttccagtt cccacaagaa tcggatgttg atcggccaac
     2941 cctgttcgat agcatatcga gcgccaaaca ttgcggtgag agccggcccc ttatcatcgg
     3001 tcgctcctcg cccccgataa attccgtttt cattcttaaa aacaaacggc gcctgcctcc
     3061 attccggctc ctgagccggc tgcacgtcca gatgattgta gatcgtgacg gtcggacact
     3121 caggtcctgt tatccacccg ccggagacaa tggggtaccc gccggtttct accacttgaa
     3181 cctcggcatt catcccggcc aagtactgcc tcgcaaggtc ggccatgcgt cgcatatcgc
     3241 cagaacgggt tgaatccata ctgattgacg gtacctctac catctgtccc aaaaaatcct
     3301 caaaccttgg acgtgactcc tttacaaagc tcctcagtcg ccgctcattc accatacgaa
     3361 tgatcctcct tgtccaaaag acgggggatt atagcgatcg cgcctgacaa gagaaagcag
     3421 cttcgggttc caaacgcgga atgatgctaa gatgcctttc agccaccatg accacttcga
     3481 cgcgaatcgt cgctcccctc ttcgtcgttc tgctcacact gataggagca aacacaccac
     3541 cactggtgtg ggcagaggaa tcgaccagta tccagcagat actggaagag ccacaggtct
     3601 accatctgcg gcatgtcact ttgcagggca cagtgcgtga tgtgcaaccc ttggcaccct
     3661 acacagtacc aaacggagat tattgttatg gggcctactt gtttcgtctc gaagaggacg
     3721 ctgctactat cccagtggca gtattgggta tttgtggcag accaattatc cgcgatccgg
     3781 aagtcgagga tggagatcgc gtcgaagtca gcgccacgat tcaggcaccg agtcatggag
     3841 ggtactacct cagtttcaaa ggattgaaag tggtgacgga gcaggaagga gtcgtgcagg
     3901 cggtggccga tcgcattctt ccgatcgtgg agtagatccg tctctccaat acatttacag
     3961 acatgctatg gagttgccgt gtaactggat catgcgatag gaaccgtgtc gaattcatca
     4021 tcattcggtt tagagataga agcggactct tctgtatgga aactagaggg ctacgcctca
     4081 ggatcggcct cgcgctgatt gtgacagtat cggccggctg tgcgagcagt caagggttcg
     4141 atcgggctgc gatgattgag gctctgcacg tagattcttc cacagagcaa gacggccaac
     4201 ctattgcaaa ccaagtctct cgcttttccc cgccgtttcg ccttggtgtc tttttcgtgg
     4261 atcacaacat cccgacagga cagtccatca gaaaaatgga atggctaagc gaggatcggg
     4321 accaacttct ccgtgaactt gcaccattgc aggatgaaca catactcacc gatacgtttg
     4381 tgctgatgga tgccactctg cgaggagagg acattcgcgc aatccgccag gcgggtgcgc
     4441 gctttggggc cgacctggtc ttgatcattg acggagtagc cgccattgat cggtacaaca
     4501 accgctctgc gtggtggtat cccaccttga tcggcgccta tctggcgcgt ggtaccgaaa
     4561 gccacgcgct tgttatgacc accggcatct tgtgggccgt gcgcttcgaa tggcacgctc
     4621 ccctccagag aaccgaagaa gcagcaaagc ttgtcggacc agcggccttc gtcgaagaca
     4681 tcgccgcact gcaggaggca aggaagcgcg ccatcgaagc tatcagcaag cgaatcgtcg
     4741 atcagctgcg gttcttggca gtaggactac ctcgcgagaa acccaactca cggtgaatcg
     4801 aagctcaaga ggactcccaa cagcagcagg tgctgccccg gtgtaagact gatttcaccg
     4861 gggcggagcc caccgtcttt gaaaaatcca cccccgactc ccgtggattc gtcgtaacgg
     4921 tgttctattc gcgtcacagt gttcatccat ttgtacggga gcttgtactc gacggtggaa
     4981 gtgatggctt tcacaaattg ctcggctcct gtccaccgac cattccggtc ccagtagaac
     5041 tcaggtcgaa gagccacaga ccaaggaccg gtaatatgcc atctcacgac catgttgcct
     5101 cccatgacga aggtacgtgg gttg
//
LOCUS       DATNDD010000078         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3540628, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000078 DATNDD010000000
VERSION     DATNDD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540628"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="carA"
                     /locus_tag="VN647_01770"
     CDS             <1..867
                     /gene="carA"
                     /locus_tag="VN647_01770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWG95784.1"
                     /translation="EAKVVAIEGIDTRALTRHLREHGSQQGLITHLDLDPDQAVRQAR
                     QAPNIIGRDLSAEVTCAHPYDWTTGTGDWAPSENGITRGTSKPWRVVAYDFGIKQNIL
                     RRLVDVGCQVTVVPAATSAVEVETFKPDGIFLSNGPGDPEGLPYAAKAMEKLIGRYPI
                     FGICLGHQILCLAFGLKTYKLKFGHHGANHPVMDLRTRKVEITSQNHNFAVEGPASLL
                     EVPKFPPVLDTRSGKLSLTHISLNDYSIEGMACLDHPIFSVQYHPEAAPGPHDSAYLF
                     NEFVRLMETHHG"
     gene            860..1810
                     /gene="sppA"
                     /locus_tag="VN647_01775"
     CDS             860..1810
                     /gene="sppA"
                     /locus_tag="VN647_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWG95785.1"
                     /translation="MDNRFVIWVIGLVLSGCTFNFPLFPGPGPLQETQVDGTGKAKVL
                     LIEISGVISSQEKDGLRSAPSMIASVKEQLTRAAKDEHVKAVVLRINTPGGTVTASDI
                     IYHELKSFKASRKVPIVASIMDLGTSGGYYIASAADTVLAHPSSVTGSIGVIMLTINA
                     RGLLEKVGVEATAVTSGPRKDMGSPFRIMTTEERAIFQGLIDSFYQRFLNIVQEGRSN
                     LQMEQIKRLADGRIYTGEQAKAAGLVDEIGYLEDAIALAKKKAGLTEAKVVTYQRPGE
                     YSNNVYSKLLTPSGFAGLADLDLMSFVRGGTPQFMYLWMP"
     gene            1891..>2771
                     /locus_tag="VN647_01780"
     CDS             1891..>2771
                     /locus_tag="VN647_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWG95786.1"
                     /translation="MTQSADQEINRLFSTPVERRALLLRGARRMLGLAWLLSFGTTGG
                     LVATLEGCVRAPGTARDQFIYISEEKEIAMGIDAYRELLRKAPISDDPELNQLVNRVG
                     KRIAAVANKPEYQWEFAIIRDDRMINAFALPGGKVAIFTGILKLTKNEGGLATVIGHE
                     VAHALQRHGAERYSRSILETIGQVGALAAGAAVGRPDAAMAAMSAYGVGVSLPFGRKQ
                     ESEADYIGLKLMAQAGYDPREAVPFWERMSGCPRQMIDKVCFRSQHNIPEFLSTHPSD
                     LSRINQIEAWIPEALKY"
BASE COUNT          631 a          716 c          806 g          618 t
ORIGIN      
        1 gaagctaagg tcgtcgcgat cgaaggaatc gatacgagag ccttgacgag acacttgcgc
       61 gagcacggat cacaacaggg actcatcact cacctcgatc ttgaccctga tcaggctgta
      121 cgacaagcca gacaagctcc caacatcatc ggtcgagatt tatccgccga ggtcacctgc
      181 gcacacccct atgactggac gacggggacc ggtgattggg cgccgtcgga aaacggcatt
      241 acacgtggaa cgtccaagcc ctggcgagtc gtggcctatg atttcggcat caaacagaat
      301 attctgagac gattagtgga tgtcggctgt caggttacgg tggtacctgc tgcgacatca
      361 gccgttgaag tcgagacatt caaaccggac ggcattttcc tctcaaacgg cccaggcgat
      421 ccggagggtc ttccttatgc cgcgaaggca atggagaaat tgatcggacg ctatccgatc
      481 ttcggcattt gtttgggcca tcagattctc tgcttggcct tcggtctcaa gacctataaa
      541 ctgaagttcg gccaccatgg tgcgaaccat cccgtgatgg accttcgaac cagaaaggtc
      601 gaaattacgt cccagaacca taattttgcc gtggagggtc ccgcgtcact ccttgaagtg
      661 ccgaagttcc cgcccgtgct tgacactcgc tctggaaaac tgtcgctcac tcatatcagc
      721 ttgaatgatt actctatcga ggggatggct tgcctggacc atccgatctt ctccgtccaa
      781 taccacccgg aggccgcgcc gggccctcat gattccgcct acttgtttaa tgaattcgtg
      841 cgtcttatgg agactcatca tggataatcg ttttgtcatt tgggtgatcg gactggtgtt
      901 gtcgggatgc actttcaatt tcccgctgtt ccccggcccg gggcctctac aagaaactca
      961 ggtcgacggg accggcaaag caaaagtgct gctgatcgaa atttcaggag tgatcagctc
     1021 tcaggaaaaa gatggattgc ggtctgcgcc cagcatgatc gcaagcgtga aagaacagct
     1081 gactcgggcg gctaaggatg agcacgttaa agccgttgtg cttcgcatca atacgccggg
     1141 aggaacggtg acggcgtcgg atattattta tcacgagctg aaatcgttca aagccagtcg
     1201 caaggtcccc attgttgcat ccatcatgga cctgggcaca tcaggaggtt actatatcgc
     1261 ctcggccgcg gacacggtcc tggcgcatcc ttcgtcggtg accggtagta tcggcgtcat
     1321 tatgcttacc atcaatgcac gagggctctt agagaaagtg ggggtggagg ccacggcggt
     1381 gacctcaggt cctcgcaagg atatgggctc gccatttcgg atcatgacga ccgaagaacg
     1441 ggccatcttt caaggactga ttgattcgtt ttatcagcgc ttcttgaaca tcgtgcagga
     1501 aggccgctcc aacttacaga tggagcagat caaacggttg gctgatggac gaatctatac
     1561 cggcgagcaa gcaaaagcgg ccggtttggt cgatgagatc ggctatcttg aagacgcaat
     1621 cgcattggcc aagaaaaaag ccggtctgac ggaggctaaa gtcgtgacgt accaacgtcc
     1681 tggtgaatac tccaacaacg tgtattccaa gctgttgacg ccgagcgggt tcgccggtct
     1741 tgccgatctc gacctcatgt cgtttgtccg tgggggaact ccgcagttca tgtacttgtg
     1801 gatgccctaa tgagggaggg gccgatcgca tgactagtag catcatgact ggtaatatag
     1861 tgacgatcgg cttcgcccca ggaatacagg atgactcagt cggcagatca ggagatcaac
     1921 agactgttct caacaccggt cgaacgtcgc gccttgcttc tgcgaggggc tcgaaggatg
     1981 cttggtttgg cttggcttct gagttttggg acaactgggg gtttggtcgc aactctagag
     2041 ggatgcgtcc gcgcgccggg aacggcgcga gatcaattca tttatatatc cgaggagaag
     2101 gaaattgcga tgggcatcga cgcctatcgc gaattgctgc gcaaagctcc gatcagtgac
     2161 gatcccgagt taaatcagct ggtcaaccgg gtcggtaagc gaattgccgc ggtggccaat
     2221 aagccggaat accaatggga gttcgctatt attcgagatg accgtatgat caatgcgttc
     2281 gcgctgccgg gcggcaaggt ggccatcttc acagggatcc tgaaactcac gaagaacgaa
     2341 ggcggtttgg cgaccgtcat cgggcatgag gtggcgcatg cgctccaacg ccatggggcg
     2401 gagcggtata gccgaagcat ccttgaaacg atcggccaag tcggggcact ggcggcaggt
     2461 gctgctgtcg ggcgcccgga tgcggcgatg gctgcgatga gcgcctacgg ggtcggggtc
     2521 tccttgccgt ttgggagaaa gcaggaatct gaagccgact acattgggct taaactgatg
     2581 gcgcaggccg gctatgatcc ccgggaagcg gttccgtttt gggaacggat gagcggctgt
     2641 cctaggcaga tgatcgacaa ggtttgtttt cgatcacaac ataatattcc ggagtttctc
     2701 tccacccatc cttccgatct gtcgcgtatc aaccagattg aagcctggat acccgaggcc
     2761 ttgaagtact a
//
LOCUS       DATNDD010000079         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3185275, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000079 DATNDD010000000
VERSION     DATNDD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185275"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..617
                     /locus_tag="VN647_01785"
     CDS             156..617
                     /locus_tag="VN647_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWG95787.1"
                     /translation="MKWLILIALLWSGPAWAGEARPLANDPAAEARLKRLAVELRCLV
                     CQNQTLADSNAPLAEDLRREVRDMIAKDMSDKEIIDFLVARYGDFVLYRPPLKATTTL
                     LWVGPFVLLTVGATALIITLRRRAHKVVDVPVTDEEHRRVEQLLAEGGKRS"
     gene            614..1906
                     /gene="ccmI"
                     /locus_tag="VN647_01790"
     CDS             614..1906
                     /gene="ccmI"
                     /locus_tag="VN647_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HWG95788.1"
                     /translation="MTATFWAIVSAMTLCVLGLLLRPLLKRPSAASSDQEKTLPVYRQ
                     QFLELEQDRANGLLTDEQYRTARSELERRVLEEAGTPETQASVVGGLVNLRFVALSLA
                     MIIPAASGVLYWTLGNPAAMTHSNVSSVASQGGPGDDSQMADSLSTLIEQLRKKLEQN
                     PNDAVGWGLLARSYMAMERYADAVPIFEKATKLNPNNASLFADYADALGVHQGRKLDG
                     KPETLIQKALKLDPHNVKALMLSGTIAYNRKDFARAAKEWEDAHAYLSPDDQESSDQL
                     KASIAEAKRHLGGGPSTNMLVANPPMEQTKPAKPATQSGQSRAITGKVVLGPNLASKA
                     SLPDTLFVFAKDVAGPPMPVSIVRASKKDLPFTFRLDDSTSPMPSRKLSDIDTVVIVA
                     RLSKSGKAMPESGDLEGMSQPVKPGTENITVVIDRERP"
BASE COUNT          482 a          530 c          597 g          455 t
ORIGIN      
        1 gccggccggg tcggtatcaa ttatggagtc tatggagtcc ctgaaaccta cgtcatcgac
       61 aagcagggtg tgatccgtta taaacagatc gggccgctgg atcccgacac cgtcgccaag
      121 acgattcttc ctcttgtgaa gcagctcgaa tcacaatgaa atggctgata ctcatagcgc
      181 tgctctggtc cggaccggct tgggctggag aagccaggcc gttggccaat gatcctgccg
      241 ccgaggcgcg actcaagcgc ctcgctgtcg agctgcgatg tctggtctgt cagaatcaaa
      301 cattggccga ttccaatgct ccgctggcgg aagacttgcg ccgggaagtt cgggacatga
      361 tcgcgaagga catgagcgac aaggaaatta tcgattttct cgtcgcgcgc tatggagact
      421 tcgtgctgta tcggccgccg ctcaaggcca cgactacact gctgtgggtg ggtccatttg
      481 tcttattgac cgtcggcgcc acagcgctga tcattacgtt gcggcgtcgg gcgcacaaag
      541 tcgtcgatgt gccggtgacg gatgaagagc atcgacgagt cgaacaacta ttggcagaag
      601 gaggcaagcg ctcatgaccg ctacattttg ggcgatcgtt tcggccatga ctctgtgtgt
      661 cctcggtctt ctgctgagac ctctgttgaa gcgcccgtca gccgcttcga gcgatcagga
      721 gaaaacgctg ccggtctatc gacagcagtt tttggaactg gaacaagatc gtgccaatgg
      781 attgctgacg gacgaacagt accgaaccgc gcggagcgag ctggaacggc gtgtgttgga
      841 ggaagcaggg acgccggaga cgcaggcctc agttgtcgga gggctggtga atctccggtt
      901 cgttgccctt tccttggcca tgatcatacc ggctgccagt ggtgtgctct actggacatt
      961 gggaaatccg gctgcgatga cgcactcgaa tgtatcatcg gttgcctctc aaggcggtcc
     1021 cggagatgat tctcaaatgg cggacagcct gagcaccttg atcgaacagt tgaggaagaa
     1081 gctcgagcag aatcccaacg atgcagtcgg gtggggactc ttggctcgat cctatatggc
     1141 catggagcgg tatgccgatg cggtgccgat ttttgagaag gccaccaagc tcaatcccaa
     1201 caatgcgagc ctctttgccg actatgcgga tgctctcggt gtgcatcagg ggcgcaagtt
     1261 ggatggaaag cccgagacgc tgatccaaaa ggccttaaag ctcgatccac acaatgtgaa
     1321 ggcgctcatg ttgtcaggga cgatcgcgta caaccgaaaa gattttgcgc gtgccgccaa
     1381 ggagtgggaa gatgcgcatg cctatctttc tcccgacgac caggagtcat ccgaccagct
     1441 aaaagccagc atcgccgaag ccaaacggca cctcggtggt ggtcctagca cgaacatgct
     1501 ggttgcgaat cctccgatgg aacagaccaa acctgccaag ccggcgacac aatccggaca
     1561 gtctcgggcg ataacgggca aggtagtatt gggcccgaat ctggcaagca aagcttctct
     1621 cccggatacg ctatttgtat ttgcgaagga tgtcgccggt ccaccgatgc cggtgtcgat
     1681 cgtacgggcg tcgaagaaag atttgccttt cacatttcgt cttgatgact caacgagtcc
     1741 catgccctca agaaagctct cggacatcga cacggtggtc atcgtggcgc gtttgtcgaa
     1801 atccggaaaa gccatgccgg aaagtggtga tctggaaggc atgagtcagc cagtcaagcc
     1861 gggaactgaa aatataaccg ttgtcatcga ccgggaacga ccgtaggtat tatttagttt
     1921 tttctcttct ttgaaaatta tgcgagattt tattcgaccg tgatccacgg aaatctcttg
     1981 acagtctagt agggctaagc tatatttcgc cgccattatt ttttgtgaac taggcgcaaa
     2041 tgaatagaga taaaaataaa aaga
//
LOCUS       DATNDD010000080         3106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1488013, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000080 DATNDD010000000
VERSION     DATNDD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488013"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..461)
                     /locus_tag="VN647_01795"
     CDS             complement(192..461)
                     /locus_tag="VN647_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWG95789.1"
                     /translation="MTDVRSGFYEHNFMTRINILQIMMASTRQQTFLTIELARLERRA
                     AEVLILICEELLRSLSMRMMGCVEALLTEETVSVQTISWITLNLD"
     gene            761..982
                     /locus_tag="VN647_01800"
     CDS             761..982
                     /locus_tag="VN647_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95790.1"
                     /translation="MRIWLVVCLCVALTCCATMETAALQDLPADWPPLGATKEATQSR
                     LGHPSSQSMMLHDGQQCEVWEWNYGSMLK"
     gene            complement(1128..1436)
                     /locus_tag="VN647_01805"
     CDS             complement(1128..1436)
                     /locus_tag="VN647_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95791.1"
                     /translation="MRIESDKTGGARLGVTGRDCLLVKKYLSWPVFLTPRSFWRKPRI
                     VERHRAGVSIIIAVVGDDIDKWQFEGQLKRGGMKRSGRADLIVTAIFYIERSEEFGYQ
                     "
     gene            2109..2261
                     /locus_tag="VN647_01810"
     CDS             2109..2261
                     /locus_tag="VN647_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95792.1"
                     /translation="MFTSVQQSKGGILDEFSRSVWQEASDVDLRIYAGKMTMVQLTVP
                     IVPLPI"
     gene            complement(2328..>3106)
                     /locus_tag="VN647_01815"
     CDS             complement(2328..>3106)
                     /locus_tag="VN647_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWG95793.1"
                     /translation="PRQEFDAESMEELEQSIRLHGVKTPVSIRPHPTEQKRWILNFGA
                     RRLRASKSVGKPTIPAFVDRSHTDYQQVIENLQREDLKPRELAIFIKRKMDEGEKQAQ
                     IAELLGVNRSMVTNHLALIDPPACIDEIYTSGKCLSAKTLYDLRNLHKEFPKDVERWC
                     STAQEITRATVSALSAKLRGPRKQQIVSSKGEQGGEERMLGSATTTTPSLIVIFQDRS
                     GTVDLHHAPQQPNHLIVHFADGKQEEVPARQCQIDQIIFQ"
BASE COUNT          714 a          834 c          798 g          760 t
ORIGIN      
        1 gctgtctgtc tctcccccgt gctgcgggtg aaggcgaaca accgctgcgt tccatcggcc
       61 aggatgtcct agacgacgaa cagccacagg cgcttacggc tttcaggccg ccgttcccat
      121 aagctgacgc catcgagaaa cagatagcgc gggtcgtccg taaatagtcc ttgatgaaac
      181 ttgaccacgg cctagtccaa gttcaacgtg atccacgaga tcgtttgcac actgaccgtt
      241 tcctcagtga gcaaggcctc cacacagccc atcatccgca tgctcagact ccgtaaaagc
      301 tcttcgcaaa tcaatatgag gacttcggcc gctcgtcgct ccaatcgcgc cagctcaatg
      361 gtgagaaacg tctgctgacg ggtgctggcc atcatgattt gcaagatgtt gattctggtc
      421 atgaagttat gctcgtaaaa gccgcttctg acatcggtca tgccggactg cgttcatact
      481 ctctcacgcc cagacaacga tttctggatt cgattgagag ccgaccaaaa agccttacca
      541 actctgccgc atctgctgag tcagatcgcc ccagcactgc tccccgagct ccatctgcaa
      601 acgttgccat tggtcggcca tggcgacagc cggagtcgag cgatccgtgc cggtaggctg
      661 atgcccagaa accagcctgg gacatggcct cgcgcagacg cgcagggtag aatgcggcca
      721 tttcttgatg gctctgcatc atctgcctaa ggagggcgtt atgagaatct ggctcgtcgt
      781 ctgtttgtgt gttgcattga catgctgcgc gaccatggag accgctgcac tccaagatct
      841 cccggctgat tggccgccct taggtgcgac gaaagaggca acccagtctc ggttaggcca
      901 tccgtccagt cagtcgatga tgctccacga tggccaacag tgcgaggtgt gggaatggaa
      961 ttatgggagc atgctgaaat gaatcctcct tatgggtccc tgtggcaggc atcgtggttg
     1021 cctcgaatag tgaaggatac aaagcgaatg gcaaggactt gatggtgacg tttattatag
     1081 tgagggcaag gtcgtttggc gttcgatctc gaaccaaggt gtggatctca ttgatagccg
     1141 aattcctcag atcgctctat ataaaaaatg gcagtgacga tcaggtctgc gcgtccagac
     1201 cgcttcattc ctccacgctt caactggccc tcgaattgcc atttatcgat atcatcgccc
     1261 accactgcta ttatgatgga gacacctgca cggtgacgct caacgatccg tggcttccgc
     1321 cagaaactcc gcggcgtgag aaataccggc caagatagat acttcttcac cagcaagcaa
     1381 tctctacctg ttacgcccag gcgagctccg ccagtcttat ctgattcaat ccgcatcggg
     1441 tacgcatcgt ttccagctct tcagacgact cggatttctc gccacgttca cccatgaaaa
     1501 cttctgcgat cttgggatct tgtttcagtt gccaatcgat gacaatgttc cactcgtaga
     1561 acttttgcct ttatttttga atcacctgaa tgatggaagt cgttgatcca caaatctcgc
     1621 tatgaagcgg gctctgcttg gcagaatgag acgtactata acgtacaacg gctcagacca
     1681 agtcataagg cgtgataatg gataaatagt gaggaaaacc gttggacatg tccaacaatc
     1741 tgtccacaga ttcatgttat tcggaccgaa ccaggatctg acggttgaat gacttcaaga
     1801 tgaagcagtc gggttatccg actatggctg gacggttggc acaatgagat tcgtcttcca
     1861 cggaggagct ctggtttgcg tcggcggctt gagtgagacc cctttccaac tatgccacaa
     1921 tctggaagtc ccgctgaggt ggtcatcagc aaaggacaca accggtttta tagaccgtat
     1981 cggtcgcgtc gaacgagtag agacagaaca tggctcagac tcaggttcac ttcagctcct
     2041 tgacaaatat tcgctgttcg tcaactctcg aagcagatct tagcgtagag tggtgatcac
     2101 ccggtgagat gttcacctcc gtccaacaga gcaagggcgg aatactggat gagtttagtc
     2161 ggtcggtatg gcaggaagcg agtgatgtcg atcttcgaat ctatgccgga aaaatgacta
     2221 tggtgcaatt gacggttccg atcgtccccc ttcccatcta ggaatgggag gggggataag
     2281 tgactatggc ctaaacgaac ttctcatgat gcaatcaaga acgggcgtta ctggaaaatg
     2341 atttgatcaa tttgacattg ccgcgcaggc acttcctcct gcttcccatc cgcaaaatga
     2401 acgatcaggt ggtttggttg ctgcggggcg tgatgcagat ccaccgttcc cgatcgatcc
     2461 tgaaatatga caatcaacga aggagttgtt gttgtcgctg aaccgagcat ccgctcctca
     2521 cccccctgct cacctttcga cgagacgatt tgttgttttc gtggacccct gagcttggcg
     2581 gataacgccg acaccgtcgc gcgagtaatc tcttgagcgg tggaacacca ccgttcgaca
     2641 tccttgggaa actccttgtg caggttccgc aagtcgtaga gcgttttcgc tgagaggcat
     2701 tttcctgacg tataaatctc atcaatgcat gccggtgggt caatgagcgc taaatgattt
     2761 gtgaccatgg accgattcac gccaaggagt tctgcaattt gagcctgctt ctccccttcg
     2821 tccatcttcc ttttgataaa tatcgccagc tcacgaggtt tcagatcttc ccgctgcaaa
     2881 ttctcgatga cttgctggta atcggtatgc gaccgatcta cgaatgcggg aatcgtgggt
     2941 ttcccgacag atttggaggc cctgagccga cgtgcgccaa aattcaagat ccatcgtttc
     3001 tgctcggtgg ggtgcggacg gatcgacaca ggcgttttga ccccgtgcag cctgatactc
     3061 tgctccaact cctccatact ctccgcatcg aattcttggc gtggct
//
LOCUS       DATNDD010000081         3991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_113375, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000081 DATNDD010000000
VERSION     DATNDD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113375"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VN647_01820"
     CDS             complement(<1..977)
                     /locus_tag="VN647_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95794.1"
                     /translation="MSFPDGFLGGHAFGSASAIRTLNEYYYLAVDGEASIYSSVPTRE
                     GMTGPFWTSLESLDPVAACKGNVVAVDLHSRLCYLKAEQTGTFPLYYALTSSGQLLFS
                     SRLKPLSRAINASPDLAGVLEFISTRHTFYRRTHYQRIFRLLPGQTITYQLDSGQLTV
                     REPRITWIGNREGRHEDLDCETLWGGLKTVVRKGFDSEKRHALMMSAGWDSRLLLCAM
                     MKELGNDRIVAYTWGDPYSRELKVVNQMCRSAGVECILEPLDDTLYEPVFLQKAFDRV
                     ENVLFPQWHRASEQLSEIGVQSVASGVYGEVLGGHYGPAMALTGMRKAIV"
     gene            complement(1389..2591)
                     /locus_tag="VN647_01825"
     CDS             complement(1389..2591)
                     /locus_tag="VN647_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWG95795.1"
                     /translation="MTNPLTKFPRFVLLSGVYALFILLGTVTAQGATYYVATTGNDSN
                     PGTSASPWRNPQRCTTSPIRAGDTCIVRSGTYTDTDGNGPVVWVYNRSSTPSGTASQP
                     ITIKSEKPLGAAIVGPSNRNGWGFLVQKPYYVIEGFDISGVNNNSSTAGGAGVAVTVP
                     GNGTIIRSNAIHHIGGQSCTTSNNGFTGVSVKDGVSGVLIEQNRISYIGRKGCVAPTD
                     AHDPAIYIRGAENTTIRRNVIYNMIRGVAIQVYGGTTTNLNIYHNTLHGYNTAQTAHI
                     LLSSTIRTANVKNNTSSDARLAMVRLYGLTASGVTISNNLSSGSMRFGSASGVTFSNN
                     IENTNPAFISKSTNDFRLTSSSPVINRGTTSGVPPVPDGRPDSSAYEYSLQSNTASPL
                     TPTGVRSL"
     gene            3486..>3991
                     /locus_tag="VN647_01830"
     CDS             3486..>3991
                     /locus_tag="VN647_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95796.1"
                     /translation="MNTHLRYMKLGSVIIAWIGVLLAATYRSHTPVVFGRYSWVYVVL
                     LGLLVGVATALSLARSTWYQPLYRVRAALVVSGVSLLLSLGAFELTLRLVDPLGLSYY
                     EQAGEYVRDKLPDDQLVFRHKPSWETRYGDVRVTYNERGLRDRPILPKAAGEYRILAL
                     GDSVTFGWG"
BASE COUNT          963 a          970 c          992 g         1066 t
ORIGIN      
        1 acgatagctt ttctcattcc tgttaatgcc atggctggac cgtaatgacc gcctagtact
       61 tcaccgtata ctcctgaggc aacgctctgc acaccaattt cggagagttg ttcgctagct
      121 cggtgccatt gggggaaaag gacgttttca acacgatcga aggctttttg aagaaaaact
      181 ggctcataca gagtgtcatc caacggttcg agaatacatt ccactccggc tgatcggcac
      241 atctgattga cgacttttag ctcacgactg taaggatctc cccatgtata agctactatc
      301 ctatcgttgc ctagttcttt catcattgca cagagtagca atctcgagtc ccaacccgca
      361 gacatcatca gtgcatgccg cttttcagaa tcaaaacctt ttctcaccac agtcttcaga
      421 ccgccccaga gagtctcaca atctaaatcc tcatgtcgtc cttcccggtt gccgatccat
      481 gtaatgcgag gttctcgaac agtcaattgg ccggaatcga gttgatacgt tatcgtctgc
      541 ccaggcagta atctaaaaat tctctgataa tgagttcgtc gataaaaggt atggcgtgtc
      601 gaaataaatt ccagaactcc ggctaaatcg ggagaagcat taattgctcg agaaagcggt
      661 ttcagacgac tgctaaacag cagttggcca cttgatgtaa gtgcgtaata tagaggaaat
      721 gtacccgtct gttctgcttt tagatagcag aggcgcgaat gtaaatcgac agccaccaca
      781 tttcccttac aagccgctac tggatctaaa gattcaagtg aagtccaaaa cggtccagtc
      841 attccttctc gtgttggcac agacgaatag atcgaggctt caccatcaac ggcaagatag
      901 taatactcat ttagcgtacg tatggccgaa gctgatccaa atgcatgtcc gccaagaaat
      961 ccgtcgggga aactcataaa tttgcagtct gtccatggtt gggagaactc tttccaaaga
     1021 ccgctatatt cctctttgtc gactcgtaga gcaccgcaaa taccaggcat aataccttgc
     1081 gtaaatattc ctatgatact ttaacagatt tttttattct aagtaaagtt gctgtgatta
     1141 tcaaaccatc atttgactat aaattgatcg agatctcatt gaactggaag aggagggctc
     1201 acagaaaagc tactgctctg gttaagactt gtctcgaaaa gatgcaacgt caattcacat
     1261 cgcagacatt cgtcttcaat atttggattg catttgcagc aatttggact acctccctag
     1321 tgcggccgcc cgatctcctg gatcgggcgg ccgtcatcgg cttcttttaa gaacgaaaac
     1381 ttctgacgtt ataagcttct cactccagtg ggagtcaggg gggatgcggt gttactttgt
     1441 agagagtatt cataggcgct ggagtccggt cttccatctg gcaccggcgg gactccgctc
     1501 gttgtgccac gattgattac gggactgctg gaagtaagtc gaaaatcgtt cgtcgacttg
     1561 ctgatgaatg cggggttggt attttcaata ttgttcgaga aggttacgcc tgaagcgctc
     1621 ccaaatcgca tgctgccact gctgaggtta ttactgatgg tcacgccaga agccgtcaaa
     1681 ccatagagac gaaccatcgc aagtctggca tcactcgagg tattgttctt aacatttgcc
     1741 gtcctgatcg ttgagctcaa gaggatgtgt gcagtttgag cagtattata gccgtggaga
     1801 gtgttgtgat agatgtttag attggtcgtt gtcccgccgt atacctgaat ggcgactccc
     1861 cgtatcatat tatagataac atttcgccgt atagtggtat tctcggcgcc tcgaatataa
     1921 attgcaggat cgtgtgcgtc agtgggtgca acacaccctt ttcttccaat ataggagatt
     1981 cgattctgtt cgatcagtac tccggagaca ccatctttaa cgcttacacc agtaaaccca
     2041 ttattggaag ttgtgcatga ttgcccgcca atatgatgga tggcgttcga tcggatgatt
     2101 gtcccatttc caggtactgt gacagcaacc cccgcacccc cagccgtgct gctgttgttg
     2161 tttactccag aaatgtcaaa tccttcgatg acatagtagg gcttctggac caggaatccc
     2221 cagccgttcc tgttactggg tccgacgatc gccgccccaa gtggtttttc gctcttgatg
     2281 gtgatcggtt gcgaggccgt gccggaaggc gtagatgacc tattatacac ccacacgaca
     2341 ggcccgtttc cgtcagtatc cgtgtaagtg cctgaacgca caatgcaggt atcaccggct
     2401 cgaatgggtg aggttgtgca tcgttgagga tttctccacg ggctggccga tgtacctggg
     2461 ttggagtcat taccagttgt tgccacataa taagtagcgc cttgggccgt aacagtccca
     2521 agaagtatga acagagcata gactccggaa agcagcacaa agcgcggaaa cttggttaaa
     2581 gggttcgtca ttatgagctc ctctagtaaa ggtacttgtt tctctccggt acaaaatgta
     2641 gtcgagcgac tccataagca cattgtatgc caatatctat agtattgatt atatttgact
     2701 gaatggctgt ggtcttattc agacgtgtaa aaagagtatg aacatgtata cagacggaag
     2761 catggctatt cggttcctga cgagtttctc atcgctatca gacgacacaa caacccgatg
     2821 tcgttcatct cttgccagga gttgtagatg attcatggaa gcgcaggaac atatcgtccc
     2881 aatataggtg gagaatgaaa atgaagatgg ggggagtgcc ggaattctcg gctggccatc
     2941 cacttaacga tatctgcaga cctagaaaca gatcgcactg gatgtgttcc aggcctattc
     3001 aatcgggcga gaagcactgg gtttgagtcg actcttatgg tattctgagc gcccatgaat
     3061 aatgagagtt tgtcgtcgca tgtcgtggga attaagctga tcgcgatcgt atcccgtgcc
     3121 caacgctttc gctagttttc tatgaatcaa gagttcacgt ccgctgacct ggagagacca
     3181 gtgctttcca cgatcattct ctggcgttac gagcttcgct gtattgagac ctgtgtacac
     3241 tcgatatttg ggaaagagcg gtcatcacgg aggaattgaa gttatcgttg gttgatggat
     3301 tcacagccga taaccctcgt gagatcttgc aaagctaccc cccaaataac attctgaatt
     3361 gcgagtcatc gataaccctt gccgcgtcat gctttgtgca atgatggtat agatgtaagc
     3421 gctaatgcct tagccttaga ggagtcgtag gtggtggtca cagtccagac tttagggttc
     3481 cagagatgaa cacacacctc cgatacatga agctaggctc cgtgatcatc gcctggatcg
     3541 gcgtcttgtt ggcggccacc taccgctctc acacccctgt ggtgttcggt cggtatagct
     3601 gggtatatgt cgtgctgctg ggtcttctcg tcggtgtcgc cacggcgctc tcgctggcga
     3661 gatcgacgtg gtatcagcct ctctaccggg tccgagccgc tctcgtcgtc agcggtgtct
     3721 ccctgctcct gtcacttgga gcgttcgaac tgactcttcg tctggtcgat ccgctcgggc
     3781 tctcctacta tgagcaggct ggagagtatg tgcgtgacaa gctgccggat gaccagctcg
     3841 tcttccgcca caaaccgtcg tgggagaccc gctacggcga cgtgcgcgtg acctataacg
     3901 agcgggggtt acgcgatcgg cctatcttgc cgaaagctgc cggagaatat cggattctcg
     3961 cacttgggga ttcggtcacc tttggatggg g
//
LOCUS       DATNDD010000082         3554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3609479, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000082 DATNDD010000000
VERSION     DATNDD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609479"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..848
                     /locus_tag="VN647_01835"
     CDS             162..848
                     /locus_tag="VN647_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWG95797.1"
                     /translation="MTALMKIDQDSLPNRLVTGLSKIGLAMKSRAWRRKGRQGIGPLQ
                     IQVLTFLRSRPNHSATVSTIARELSVKLPTASEVIRTLEHKRLVRRRRREIDNRVVTV
                     HLTSLGAKAGHVENRWPEILASATENLSVQEQVALLTALVKLIRALQLQGEIPVARMC
                     VSCEHFHPHAYPESDQPHHCDFYNAAFGDQAFRLDCPEYVEAPAEGPSQNADSHKTAS
                     MTQTQGLTTA"
     gene            complement(889..1425)
                     /locus_tag="VN647_01840"
     CDS             complement(889..1425)
                     /locus_tag="VN647_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95798.1"
                     /translation="MIHSIAFKMLLLSLCVTWMGCVHRIQVTPIPASASSITIPRTLQ
                     AVISPISMEGPDHRPGIVLLEWPHLDLKQAVLQYLQQRGTFASVSFDPADLTLRVATN
                     LTLTSRSNLYHYRIGLQAEMSEGVRSIKSYRAEHTAVGSSVRWVTASDRGPIEIALQA
                     ALEDLMRQVEVDRPIYHR"
     gene            complement(1489..1974)
                     /locus_tag="VN647_01845"
     CDS             complement(1489..1974)
                     /locus_tag="VN647_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95799.1"
                     /translation="MKHSGHYFQHQTVGLIAMVVLLISSGCAQDSYQRRADVMKDHIE
                     NFYAHLRADRVEAAVHENEQIEVMADRMADTVRKQGQLQGTSQVGREFALMKTARDTA
                     AQNWIALGQYFAIKQQPERARASYQRVVDTYMHPTERAYREQAARALKDLEIVSGPAP
                     Q"
     gene            2204..2629
                     /locus_tag="VN647_01850"
     CDS             2204..2629
                     /locus_tag="VN647_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HWG95800.1"
                     /translation="MGKSLKGTKSHDNLKHAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPMGNTDANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFPELAEWFETLAKAERSHANRFQKGLDTLKA"
     gene            2723..3478
                     /locus_tag="VN647_01855"
     CDS             2723..3478
                     /locus_tag="VN647_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWG95801.1"
                     /translation="MKGISLITPIDSTQLEKETLRIYEVCDGCRRCFNLCPSFNTLLG
                     RIDAYDGAVAGLTPADHHQIVDECYYCKLCFNHCPYTPPHHYEIDFPHLMVAWKKHLA
                     AERGVPFRDRLLIMTDTIGRLGSAIASIANRLLENRLVRGLLERLMGIHRDRRLLHFS
                     GETFPRWFGRRTKTVIAAPSVRKVALFASCLVNFQATDVGKATVQVLEKNGARVVVPE
                     QRCCGMPSFDIGDTQAIQQAIRSNVASLYPWVL"
BASE COUNT          771 a          949 c         1021 g          813 t
ORIGIN      
        1 cgtttccact cgggtggtgc aacatgccca ctgtggtgtg ctcgtggtcc gctgatcatc
       61 ttaagtaggg gatggctcat ggcagcctaa acagacttga cggcaaggag gtaggggcaa
      121 tagactgacg ccctctcgga tactccaagg gagaggctat tatgacagca ttaatgaaaa
      181 tcgatcaaga ctcacttcct aatcgtttag tgacggggct ttcaaaaatc ggtttggcga
      241 tgaagagccg cgcatggaga cggaagggac gtcaaggtat tggtcctctg caaattcaag
      301 tgctgacgtt tctccggtct cgaccgaatc attcggctac cgtctcgacg attgcccgag
      361 aactctccgt taaactgcct accgcttctg aagtcattcg aacgcttgag cataaacgtt
      421 tggtccgccg gcgccgtcgg gaaatcgaca accgcgtcgt gaccgttcac ctcacctcac
      481 ttggggccaa ggcaggtcat gtggaaaacc gatggccgga gattctggcg tctgcgaccg
      541 agaatttgtc ggtgcaggag caggtcgctc ttctgaccgc gctcgtgaag ctgatccgcg
      601 cacttcaatt gcagggtgaa attcctgttg cacgcatgtg cgtctcctgc gaacatttcc
      661 atccgcatgc ctacccggaa tccgaccaac ctcatcactg cgacttctac aatgctgcgt
      721 tcggggatca ggcattccgt cttgattgcc ccgagtatgt ggaagctccg gcggaaggtc
      781 cgagccagaa tgccgactct cacaaaactg cgagcatgac ccaaactcaa ggcctaacca
      841 cagcctgacg gtttatcatc gggcaacttc tgtgacggtt gctctgttct accgatggta
      901 gatcggtcgg tccacttcga cctgcctcat taagtcttcc aaagccgcct gtaacgcgat
      961 ttcgatggga ccacggtcgg aagcagtgac ccaccgtacc gatgatccca ctgcagtgtg
     1021 ttcggcgcgg tacgacttaa tagatcggac gccttcgctc atttctgctt gcaacccgat
     1081 tcgataatga tagagattgc tgcgggaggt cagcgtgagg ttcgtggcca cacgaagcgt
     1141 caggtcggct gggtcaaacg agactgaagc gaaggtgcct cgttgttgca ggtattgcaa
     1201 gaccgcctgc ttcagatcga gatgcggcca ttccagcaag acaatgcccg gccgatggtc
     1261 cgggccttcc attgaaatcg ggctgatcac ggcctgaaga gttcgaggga tggtgatgga
     1321 cgacgcgctc gcggggatgg gagtcacctg aattcgatga acacacccca tccatgtcac
     1381 acagaggctc agcaagagca ttttgaatgc aatggagtgg atcatagata atcagaggtc
     1441 ggcgtacgaa ctttcgccac aatgtttcga tctgtgagag gagtgagtct actggggagc
     1501 gggccctgac acgatctcca agtctttcaa tgctcgagca gcctgttccc ggtaggcgcg
     1561 ttccgtcggg tgcatatagg tatcgaccac acgttgataa gaggcgcggg ctctctccgg
     1621 ctgctgcttg attgcgaaat attgtcccaa cgcaatccag ttctgagctg ccgtgtctcg
     1681 ggccgttttc atcagcgcga actcccgccc gacctgcgac gtcccctgca actgaccttg
     1741 cttcctgacg gtgtccgcca tccggtcggc catgacttcg atctgttcgt tttcgtgaac
     1801 cgccgcttcc actctgtcgg ctctgaggtg cgcatagaag ttctcgatgt ggtccttcat
     1861 gacatccgct cgccgctgat acgagtcttg tgcacacccg gacgagatga ggaggacgac
     1921 catggcgata agaccgaccg tttggtgctg gaagtaatga ccggaatgtt tcatgggtcc
     1981 tcttgaatga aagttctcga gcatatcatc tcggggagtc taacatacgt ctgagcacta
     2041 gccaaatcag tgggaattct cgaaacggcc ttggtgcagt ctgggtgagc cggtgaagcc
     2101 tcttgaacct ctcacaatgg ctgagctatc ttagttcccg gtttccatag actggaaatt
     2161 cagggggcat aggctgtggg gcttcgaata aggaggagat gacatgggaa agagcttaaa
     2221 agggaccaaa agtcacgaca atctgaaaca tgcgtttgca ggcgaatcac aggccaatcg
     2281 ccggtatctg tatttcgcgc ggcgggccga tattgaaggc tatccggacg tcggcgggct
     2341 tttccgggac acctcggagg ctgaaacggg ccatgctttc ggccatctgg attttttgaa
     2401 agaggtggga gatccggcaa cgggcgtgcc gatgggcaat acggatgcca atctcaagtc
     2461 cgccatcgag ggcgaaacgt acgaatatac tcaaatgtat cccgggatgg cgaagaccgc
     2521 gcgagacgaa ggttttccag agttggccga atggtttgag accttggcga aagctgaacg
     2581 gtcccatgcc aatcggtttc agaagggatt ggatactctg aaggcctaac agtctgctgt
     2641 cagctcacgt gcagcggtca gttttcgacg tgacgggaat ccgtcggcga agactggccg
     2701 ctggtcgttt tcggagagac gaatgaaagg catcagcctt atcacaccga tcgattccac
     2761 gcagttggag aaggaaacgc tgcggatcta cgaggtctgt gacggctgtc ggcgctgctt
     2821 caatctctgt ccgtcgttca acacgttgct gggccggatc gatgcgtacg acggtgctgt
     2881 ggccggactg actccggccg atcatcatca aatcgtcgat gaatgctact actgcaagct
     2941 ctgtttcaat cattgtccct atactccgcc tcatcactac gaaatcgact tcccgcattt
     3001 gatggtcgcc tggaagaagc atcttgcagc ggagcgcggc gttccgtttc gggatcgcct
     3061 actgatcatg accgatacta tcggaagact cggcagtgca atcgcctcca tcgccaacag
     3121 gttgttggag aaccgacttg tgcgcggcct tttggaacga ctcatgggga ttcatcggga
     3181 ccgtcggctc ctgcattttt ctggagagac gttcccccgg tggttcggcc gtcgaaccaa
     3241 gacagttata gccgctccat ccgtccgcaa ggtcgccctg tttgccagtt gcctcgtgaa
     3301 ttttcaggcg accgacgtcg gcaaggcgac ggtacaggta ctggagaaga acggggcccg
     3361 ggtggtggtg ccggaacagc gctgctgcgg catgcccagt ttcgatattg gggataccca
     3421 ggctattcag caggctattc gaagcaacgt cgcatcattg tacccctggg tcctctaggg
     3481 gtacgatata gtagtcccga ccgccagttg cagtttgatg ctgaagcgcg aatatccgga
     3541 gcttcatcgc gatg
//
LOCUS       DATNDD010000083         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3062050, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000083 DATNDD010000000
VERSION     DATNDD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3062050"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VN647_01860"
     CDS             complement(<1..1364)
                     /locus_tag="VN647_01860"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HWG95802.1"
                     /translation="MLTASDVTEVLTEISPLLRGGWIQKIQQPAGHTLVLDVRVPGQT
                     HRLLICCHPNLARLHVTTHSLANPPTPPPFCQFLRAHFQGARIDDICQIDNDRIVELQ
                     ITGREGAHAIVCELTGSKANILVLDAERRVLRDLTRQFRLAGQAYIPPSQRNTNHKTV
                     PSRFAGKVQGLLPVSEAVDAHYREQEATQTIDRIKADRLRTLKKTLKKEQRLIEAWRN
                     DLSRATAYRDYARYGELIKANLSAIIKGTDHIEMTDYFDDRLPTITIPLDPMKSPQGN
                     MDDYFRKHRKFLAAERELKPRIERAEHGIERLRQEIQQIEQGTWSPPVVSPARLNAAA
                     PVQATVSKGHLATDQRRGPFRRFISADGLPIFVGRNARENDELTFGLAKSDDLWLHAR
                     GTTGSHVVVRLSKGTDPPPETLRDAAMLALLYSDLKKSGKGEVIYTRRKWVKKAKGQA
                     PGAV"
     gene            1642..>2668
                     /locus_tag="VN647_01865"
     CDS             1642..>2668
                     /locus_tag="VN647_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95803.1"
                     /translation="MRLLDLQQLTQLRDEFRLSMRRLFADLCREVHADHADLGRELGV
                     PTDFFDRLHSSLRPGAYSNWKVVGWIETLNDLVYLLDVFRQLKREQDRPEFAEQLLDE
                     CQEKFFEHGYLDELFPAGQPHATGLEKRLFGLCRKLAQELVQESLWFDSALPVKWCRQ
                     RKIGHWDVLGTLTDSFERGEPAGTIAAGIAGAWCGAPKNVRQELAHPSRRVIFRVEPI
                     GITVRVGKIVSPIWSGWGRAGQWQWTYHPPIVAAHSGQDPVTVGPTLVYGRDRQPRTL
                     KPTDQRQIARIARAWQTIQHAWPEGHEVLMLLTSRIVPLQAKGVVSFSYRHRPGLSFI
                     NCFDRGNL"
BASE COUNT          545 a          703 c          777 g          643 t
ORIGIN      
        1 atcacggcgc caggcgcctg tcccttcgcc tttttgaccc acttgcgccg cgtgtagatc
       61 acctcgccct tgccgctttt cttgagatca ctgtagagga gcgccaacat ggcggcgtca
      121 cggagggttt ccggtggagg atcggttcct ttgctcaatc tcacgacgac gtgcgagcca
      181 gttgtcccac gagcgtgcag ccagagatcg tcgctcttgg cgagaccgaa cgtcagctca
      241 tcattttctc gggcattgcg tccgacaaag atcggcagcc catccgccga tataaaccgg
      301 cggaacgggc cgcgtcgctg atccgtggcg agatgcccct tgctgacggt cgcttgaacg
      361 ggagcggctg cattcagacg ggcaggggat acgacagggg gactccaggt tccttgctca
      421 atctgctgga tctcctggcg tagccgctct atgccgtgct cggcccgctc gatacgcggt
      481 ttgagttcac gttcggcggc aagaaatttt cgatgctttc gaaaatagtc gtccatgttg
      541 ccctggggtg atttcatggg atcgagtgga atggtgatgg tcggaagccg atcatcgaaa
      601 taatccgtca tctcaatatg atcggttccc tttatgattg cgctaaggtt agccttgatc
      661 aattctccat accgggcata gtctcgatac gcggtggctc ttgataggtc gttccgccag
      721 gcctcaatca gtcgctgctc ttttttgagc gttttcttga gggtacgtag acggtctgct
      781 tttatccggt cgatggtttg tgtggcctcc tgttcgcggt aatgcgcatc gaccgcctct
      841 gaaacaggga gcaggccttg gactttacct gcgaaacggc tgggcaccgt tttgtggttg
      901 gtattgcgtt gagatggtgg gatatatgcc tgtccggcga gacggaattg acgtgtgaga
      961 tctctcaaca ctcggcgttc ggcatcgagg accaggatat ttgccttgct gccggttagt
     1021 tcacacacga ttgcatgtgc tccctctctc ccggtgatct gcagttcaac gatacggtca
     1081 ttatcaatct gacaaatatc gtcgatccgc gctccctgaa aatgggctct gagaaattgg
     1141 cagaacggtg gaggtgttgg aggattagct aatgaatgtg tggtgacatg caaccgagcg
     1201 aggttcggat ggcagcagat cagcagtcga tgggtctgtc ccggcacgcg tacgtccagc
     1261 accaaagtat ggccggcggg ttgttggatc ttctgaatcc atcccccgcg caggagaggg
     1321 gaaatctccg taagaacctc tgtgacatcc gacgcggtca acataacaag tccatgccac
     1381 cgtcggcccg taggctcaac ctgcctcaat tcggtctcgt gaggataatc tggtctgcgg
     1441 tcttttgtcc gcactgtacg gcctactgca acaagtcgca agagtaccag gaaggtttct
     1501 gacatcatca ttacgtgtcc taattaatcc atgaattgcg gattggtcac aacgtcggca
     1561 tagccggtca ggcttctcgc ttgcgcctac cctcctgcta cgctagagtt cttccggcat
     1621 ttcacgcgta tccgttccgc catgcgcctt ctcgatttgc agcagcttac gcaattacga
     1681 gatgagtttc gtctgtcgat gcgacggttg tttgccgacc tgtgccgaga agtgcatgcc
     1741 gaccatgctg atctggggag agaattgggg gtcccaaccg acttctttga ccgtctccac
     1801 tcaagcctca ggccaggagc ctattccaac tggaaagtcg tcggatggat cgagacgctg
     1861 aatgacctcg tgtatctcct ggatgtgttc aggcagctca aaagagaaca ggatcggcct
     1921 gagtttgcag aacaattgtt ggacgaatgt caggagaagt tttttgaaca tggttatctg
     1981 gatgagctct ttccagcggg tcagcctcac gcaacgggat tagagaaacg gttgttcggg
     2041 ctgtgccgga aattagcaca agaattggtc caggagtccc tatggtttga ctcggcgttg
     2101 ccggtcaaat ggtgccgcca gcggaagata ggacattggg atgtgctggg aacgttgacc
     2161 gacagcttcg agcgtggaga accagccggg acgattgctg ctggtatcgc cggtgcatgg
     2221 tgcggggcgc cgaaaaatgt tcgacaggag ttggctcacc catcaaggcg tgtgatattt
     2281 cgagtggaac caattggcat aacggtgagg gtcggtaaaa ttgtctcacc aatctggtcg
     2341 ggttggggac gagcgggaca atggcaatgg acataccatc caccaatcgt cgccgcccac
     2401 agcggtcagg atcccgtcac cgtcggtccg actcttgtct acggaaggga tcgtcagccg
     2461 agaaccttaa aacccacaga tcagcgacag attgcacgga ttgctcgggc ctggcagacc
     2521 atccagcacg cttggcctga aggccatgag gtgctgatgc tgctgacttc tcgcatagtc
     2581 ccgctgcagg cgaagggggt ggtcagcttc agctaccggc accgacccgg actgtcgttc
     2641 atcaactgtt tcgatcgtgg caatctcg
//
LOCUS       DATNDD010000084         3702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4280374, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000084 DATNDD010000000
VERSION     DATNDD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4280374"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VN647_01870"
     CDS             complement(<1..676)
                     /locus_tag="VN647_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWG95804.1"
                     /translation="MMHPTSILVESSKGYARVTLNRPDRRNAFDACMVEELCEIFKTL
                     GQDHSVRAIVLTGRGPTFCAGVDLRWMGIDRSLSTSEARRDAEQLLAMFRTIDDCPCP
                     VIGCVQGSVYGGGIGLVAACDIAVAAASATFAFSEVRLGLVPAVIAPFVLTKVGDSFV
                     RRFCLTGEPFPASTAQGNGLIHDVVEPAALEAHIASLTERIIHLAPQAVRDTKALFRR
                     LHHLSRE"
     gene            complement(684..2288)
                     /locus_tag="VN647_01875"
     CDS             complement(684..2288)
                     /locus_tag="VN647_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWG95805.1"
                     /translation="MRTLTTSVVAASGEFASNRAHYEGLVSDLQKHLTLARSGGSPEA
                     TAIHQQRGKLTARERIAKLLDPNAPWLELSPLAASDMYDDHIAAAGLITAIGFVAGRP
                     CVIAANDATVKGGTYFPMTIKKHLRAQEIALENRLPAVYLVDSGGVFLPMQADVFADK
                     EHFGRIFYNQARLSALGVPQIAVVMGMCTAGGAYVPAMCDENVIVKGTGTIYLAGPPL
                     VKAATGEEVTAEELGGADLHTRLSGVSDHLADDDHEALEICRSIVETLPQRPVLRRPA
                     PIEEPQYPASELYGLIPNNPRQTFDAHEVIARLVDGSRFHEFKARYGRTLICGFARWM
                     GHQVGIIANNGVLFSEAALKGAHFVQLCAQRRLPLVFLQNITGFMVGKEYETRGIIKD
                     GAKMVQAVATADVPKFTIIIGASHGAGNYAMCGRAYAPRFLFLWPNARTSVMGAQQAA
                     QVLLTVKRQQRTRDKASLSEDEQRKIMDSIRTQYEREGSPYFSTARLWDDGIIDPVDT
                     RNILGLCLDVAMTTPVGSSRFPVFRM"
     gene            complement(2295..2702)
                     /locus_tag="VN647_01880"
     CDS             complement(2295..2702)
                     /locus_tag="VN647_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWG95806.1"
                     /translation="MTVASAPLRILIGKVGLDGHDRGVKLIARALRDAGVEVIYTGLH
                     QTPEQVVNTAIQEDVDAIGLSILSGAHKTLFRRVLELLKEQDAGDIALFGGGIIPDED
                     VAALTADGVKALFRPGTPFQEIVTFAKGLKPQG"
     gene            complement(2699..3538)
                     /locus_tag="VN647_01885"
     CDS             complement(2699..3538)
                     /locus_tag="VN647_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWG95807.1"
                     /translation="MKLGAFEIYPVSDGRFRLDGGAMFGVVPKILWEKCCPADDLNRI
                     SLSLTALLIRANGKNILVDTGLGPKEDEKFRNMFAVERKPTILESLKRLGLGPEDIHL
                     VINTHLHFDHAGGNTMQENGTVVPTFPNAKYFVQHGEFEDAARANERTKASYRRDNFT
                     PIDHINQWEFLHGDTELVPGVTTVVTAGHTRWHQSVKIESEGQVAFFLGDLIPTVSHL
                     PLPYIMGYDLFPIQTLETKRWVSDRAFEERWLLLFEHDPVVQAGYVRRDQDGKYFLHE
                     VAA"
BASE COUNT          797 a         1109 c         1017 g          779 t
ORIGIN      
        1 cctctcggga caaatggtga agtcggcgaa acagcgcttt cgtgtcccgt acggcctgcg
       61 gagcgagatg gatgatccgt tcagtcaatg atgcgatatg ggcctctaac gcagccggtt
      121 cgactacatc atggatcaga ccgttgccct gagcggtgga tgcgggaaat ggttcaccgg
      181 ttagacagaa tcgtcgcaca aaagagtccc cgaccttcgt aagaacaaag ggcgctatca
      241 cagcggggac caatcccaag cggacctcgc tgaacgcgaa tgttgcactt gcagcggcta
      301 cggcaatgtc gcacgccgca accaacccga ttcctccgcc atagacggac ccctgaacgc
      361 atccgatcac gggacagggg cagtcatcga tggtccggaa catcgcgagc aattgctcgg
      421 catcccgccg cgcttctgat gtggacaggc tccggtcaat ccccatccag cggaggtcta
      481 ccccggcaca aaatgtcggg cctctaccgg tcaagacgat tgcacgcact gagtgatcct
      541 gtcccaatgt cttaaagatc tcgcacagtt cctccaccat gcaggcatcg aacgcgttcc
      601 gccgatcggg ccgatttaag gtcacacgtg cgtatccctt actcgactca acgaggatcg
      661 acgtaggatg catcataggc gcctcacatg cgaaaaacgg ggaagcgcga cgagccgacc
      721 ggcgtcgtca ttgcgacgtc aagacacaga ccgagaatgt tccgtgtatc cactggatcg
      781 atgatcccat catcccagag ccgtgccgtg ctgaaatagg gactcccctc ccgctcatac
      841 tgtgtacgaa tggagtccat gatctttcgc tgctcatctt ccgacaggga cgctttgtcg
      901 cgggtccgct gctgccgttt caccgtcaag agtacctggg ccgcctgttg ggcccccatc
      961 accgacgtcc gcgcgttggg ccagagaaaa agaaagcgcg gagcataggc ccgtccgcac
     1021 atggcgtaat taccggcacc gtgagaggcg cctataataa tggtgaattt cgggacatcg
     1081 gcagtcgcca ccgcttgcac catcttggct ccatccttga tgatccctcg tgtctcatac
     1141 tccttaccga ccatgaagcc tgtgatgttt tggagaaaca ccagcggcag ccgccgctga
     1201 gcacagagtt ggacaaagtg ggcgcctttc aaggcggcct cggagaacag cacgccgttg
     1261 ttcgcgataa tgccgacttg gtgccccatc caacgggcga agccgcatat gagcgtccgt
     1321 ccgtagcggg ccttaaactc atgaaagcgg ctgccgtcca ccaagcgcgc aatgacttca
     1381 tgggcgtcga aagtctgccg tggattgttc ggaatgagtc catacaactc agaagccggg
     1441 tactgcggct cttcgatcgg tgccggtctg cgaaggaccg ggcgttgagg caatgtctcg
     1501 acgatcgacc ggcagatctc gagcgcttca tggtcatcgt ctgcaaggtg gtcgctcaca
     1561 cctgagagcc tggtgtggag atcggccccg ccgagttctt cagccgtgac ctcttcgccg
     1621 gttgccgctt tgaccagtgg cggccctgcg agataaatgg tgccggtccc tttgacgatc
     1681 acgttctcat cgcacatggc cggcacgtaa gcaccaccag cagtgcacat ccccatgacg
     1741 acggcgattt gcggaacccc aagggcagac aggcgcgcct gattgtagaa gatccgtccg
     1801 aagtgctcct tgtcggcaaa gacatcggcc tgcatcggca gaaacacgcc gcctgaatcc
     1861 acgagataga ctgcgggcaa tcgattctcc aacgcaatct cctgtgcccg gagatgtttc
     1921 ttgatcgtca tggggaagta ggtcccgccc ttgaccgttg catcgttggc ggcgatgaca
     1981 caaggccgtc ccgccacaaa gccgatggcg gtaatcagac cggcagcagc aatatggtcg
     2041 tcgtacatgt ccgatgccgc caaggggctg agttccagcc aaggtgcatt tggatcgagc
     2101 agtttcgcaa tccgctcccg agcggtcagt ttgccacgtt gttgatgaat ggcggttgct
     2161 tccggtgacc ctccggatcg agcgagggtg agatgtttct ggagatccga caccagaccc
     2221 tcatagtgcg cacggttcga cgcgaattcg ccggaggcgg caaccactga agtcgtcaac
     2281 gtacgcatgg tttttcagcc ctgtggtttg agccctttgg cgaacgtcac gatttcctgg
     2341 aaaggcgtgc caggcctgaa gagagccttc acgccgtccg ctgtgagcgc cgcgacatcc
     2401 tcatcgggaa tgattccgcc gccgaacaag gcgatgtctc ctgcatcctg ttccttgagg
     2461 agttcgagga cgcgtcggaa cagcgtctta tgcgctcctg aaaggatgct caagccgatc
     2521 gcatccacat cctcttggat cgccgtattg acgacctgtt ccggcgtctg atgcagaccg
     2581 gtatagatga cttccacccc ggcgtctcgt aatgctcgcg cgatgagctt cacacctcga
     2641 tcatggccgt cgagaccgac tttaccgatc aaaatccgca gcggagcgct cgctactgtc
     2701 atgccgccac ctcatgaaga aaatattttc cgtcctgatc cctccgaacg tatcccgcct
     2761 gaaccactgg atcatgctca aagagcagca gccatcgctc ttcgaacgcc cgatccgaca
     2821 cccatcgttt cgtctccaaa gtctggatgg gaaagagatc gtagcccatg atgtaaggaa
     2881 gcggcagatg cgacacggtc ggaatcaggt cgccgaggaa aaacgccacc tgtccttcag
     2941 attctatctt gacgctttga tgccagcgcg tatggccggc ggtgacgacc gtagtcacac
     3001 ctggaacaag ttccgtatcg ccatgcagaa attcccattg attgatgtga tcgatcggtg
     3061 tgaaattatc tcgccggtag ctggctttag tccgttcatt ggcgcgagcc gcatcttcaa
     3121 actcgccatg ttgaacgaaa tatttggcat ttgggaaagt gggtacgacg gtcccgttct
     3181 cttgcatcgt attcccgcca gcatggtcga aatgcaggtg tgtattgatc accagatgga
     3241 tgtcctccgg tccaagacct aaccgtttca acgattcaag gatggtcggt ttccgctcga
     3301 cggcgaacat attccgaaac ttctcgtctt ccttaggacc cagcccggta tccaccagga
     3361 tattttttcc gttggctcga atcagcaaag cggtgaggct gagggagatt ctgttcaagt
     3421 catcggcggg gcaacacttc tcccacagga tcttcggcac aacaccgaac atagcacccc
     3481 catccagccg aaatcgcccg tcactcacgg ggtaaatttc aaatgcaccg agtttcataa
     3541 ctctctctcc cccctaagcc tcggttgaaa gggtatggga taccgagcgg gcgccatatc
     3601 ctctctctca tccccttcgg cggcgatgat cagaatgcct cctgtgctcg cgcgacgcgc
     3661 acacggggct gatcagattg tgatcacagc gggcggtgct cg
//
LOCUS       DATNDD010000085         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_787312, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000085 DATNDD010000000
VERSION     DATNDD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787312"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VN647_01890"
     CDS             <1..298
                     /locus_tag="VN647_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95808.1"
                     /translation="GLGREFEGVATTGEYSAIMEELAQRIRGGEVFIAASGTLVYEDI
                     FRDTYEVPYSFVWHSFGNDDGRTWLIESDWLDLSPRERRVRNDKTQEDKSQNPN"
     gene            453..1043
                     /locus_tag="VN647_01895"
     CDS             453..1043
                     /locus_tag="VN647_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95809.1"
                     /translation="METRTADYTPIFTTREFIRTPAHLGYEKVGAITQSKTDPNLKMI
                     AMKVRNIGRGPAVLLEVWHQPITPKFPAGLIGVLTPTGPAEKGAIDLHELMPSEVAPI
                     VFGPYDTRRRWLFVISCIDTIGGRHQLQIVAEVDGTSQWSMMHNRGNSWQERRREVHH
                     QVHRRVGGNRQTIETADFLERITALRQPRRSRQAKR"
     gene            2010..>2462
                     /locus_tag="VN647_01900"
     CDS             2010..>2462
                     /locus_tag="VN647_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95810.1"
                     /translation="MSINGTGSYRGGPDQLVSVRATCHTIDSPPFYALHSGYGFVDPD
                     NTTHIMAPSLVVGNTLGQDKPCWRAMDNGIFYAQDGSGWALVNGTPTSKSGEMAGWED
                     ANGNTLQNNGSQILDTVGRIYTTDGSYYDESGTLQRIGVQTEQVSISTH"
BASE COUNT          585 a          668 c          652 g          557 t
ORIGIN      
        1 cggactggga cgagaattcg aaggagttgc cacgactgga gaatacagcg ccatcatgga
       61 agaactggcc caacggattc gtggcggaga ggtattcatt gcggcgagtg gcacgctggt
      121 ctacgaggac attttccgcg acacctatga agttccgtat tcctttgtgt ggcactcctt
      181 cggaaatgac gatggaagaa cgtggctgat tgaatccgat tggcttgacc tcagcccacg
      241 cgagcgccgg gtccgcaacg acaaaaccca agaagacaag agccagaatc caaattagga
      301 caccgcccga atccggtagt ctggctcaat atgcctatgt ttctacaccg aaccgagttt
      361 tttggatagg atcctctgca atttcatcgg cggcaaccct gctcgtggtc tttctataca
      421 ccctatatac gcggaagatg atgcgtatgc agatggagac cagaacggcg gactacaccc
      481 ctatcttcac cacgagggag ttcataagga caccagctca tctgggatac gagaaggtcg
      541 gtgcgattac ccagtccaaa accgatccaa atctaaagat gattgcaatg aaagtcagaa
      601 atattggccg tggccccgca gtgctgctgg aggtatggca ccagccaatc actcccaaat
      661 ttccggcggg actaattggc gtcttgactc cgactggacc cgccgaaaag ggggcaattg
      721 atcttcatga gttgatgcca agtgaagtag ctccgatcgt cttcggtccg tatgacactc
      781 gaagaagatg gctttttgtg atctcgtgca tcgatacgat cggaggacgc catcagcttc
      841 aaattgtcgc tgaggtcgat ggaacctcgc agtggtcaat gatgcacaac cgaggaaata
      901 gctggcaaga acggcgtcgt gaggttcacc accaagttca tcgacgtgtt ggaggcaatc
      961 ggcaaacaat cgaaacggct gatttcctag agcgaattac cgccttgcgt caacctcgac
     1021 gatccaggca agcaaagaga taactgcgct gcctccgaca ggccgctccg acaacccatc
     1081 accgccacgg gttgagcgat tcgcccgcgc gttcaaacag gagcgattct cccatgctgc
     1141 ccattgccga ccagcgtaca gatctgcgtt tctaacccgc gccaaccgct cagcgttcgc
     1201 ctttccgcga tctttcgtgc caccgtctca cactgctttt tcgaaacagg cgatggtttg
     1261 tcttagcgtc gactcgattc gagcgccacg gcgaccaaag gcaatgcctg gaacatctca
     1321 tcggcgatct cgtcgtgagt tcgtagtact gctgtcgatg tcccgaggcg ctcgctgcac
     1381 ttgggtgtga ggctctggtc tacttcccgg ctaggaagtg caccagagtg gcgattcagc
     1441 taaacggaag ctggatcgcg agattagcct gagatcacac cacaatccca agttggcctc
     1501 ttatggcagt gaggtgacgg ccctcggcta gtgccacccc gagggtcgtt gtatggtggc
     1561 ctcctgctgt cctaaacccg ttactaaagt tctcctgtaa agtcactttc ttctggactt
     1621 agggtagggt catcggtatc tttctcccga aatccttcag cgccgttttg agcactctta
     1681 attcgctttt ctggagcgtc atgagagcca atctcgccgt gtctgctttc ctccttgttt
     1741 ttgtcgggaa aatcgtggcc cagtcaacat cggaagttca caatgttggc ttcccaatga
     1801 acggaatctt ccaaggctcc tccgtggata gtgttcaacc gaacaacgga aatcttcacg
     1861 tgagaatacc gctgtgggaa ctcccagggc gcggtctctc cacgaagtct gcgttcattt
     1921 acgacagcat ggggtggcat ctcgacgccc agtgtagcga ttccgggtgt acgtatggtg
     1981 tagtgcctca gcaggtaaac acaatgcaga tgagtatcaa tggtaccggt tcttatcggg
     2041 gtggacccga ccaactcgtc agcgtgaggg caacctgcca cacgattgat tcaccgccct
     2101 tttatgctct tcattcgggt tatggattcg tcgatcccga taacaccaca cacatcatgg
     2161 ctcccagtct cgtagtcggc aacacgctgg gacaagacaa gccatgttgg agagcgatgg
     2221 ataatggcat cttctatgca caagacggct cgggatgggc attagtcaac ggcactccta
     2281 catcgaagtc cggtgagatg gcgggctggg aggatgcgaa cggcaacaca ctccagaaca
     2341 atggctctca aatcctcgat accgttggaa gaatttacac gacagatggt agctactatg
     2401 acgagagtgg aacgctgcag cgtatcggcg tacaaaccga acaggtgtca atctcgacgc
     2461 ac
//
LOCUS       DATNDD010000086         4503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2973941, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000086 DATNDD010000000
VERSION     DATNDD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2973941"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VN647_01905"
     CDS             <1..387
                     /locus_tag="VN647_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95811.1"
                     /translation="REARKAKMDLSRVWAVLPAERDFTPLALGISDEAKRAQVTLPAL
                     SYKTEPTLVANTSKGLLQGPMTGRYEDLRRFIYGLETAEELLFIEDLELTRAGGARDA
                     LLTFNIRIATYLRGDTGESGLPRLAQ"
     gene            390..986
                     /locus_tag="VN647_01910"
     CDS             390..986
                     /locus_tag="VN647_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95812.1"
                     /translation="MDAKSKTILAASLIFLWAGLAVWQWRLLQEPVRIPLTNVTGHAP
                     VGRQVETGRAGLHVNLDLLASTGLQRQATFTVPRNIFAVPRSDGTFAAENATAFVSQP
                     SASAKTMVQQEDALELGQYRYLGFLRMGEKRGNNNDIAVLRKDDEVLVLRVGDRVDDH
                     LILKAINSESVTIRNTGTRSDQWIPLSEESSEEPMAQE"
     gene            993..1700
                     /locus_tag="VN647_01915"
     CDS             993..1700
                     /locus_tag="VN647_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95813.1"
                     /translation="MWSLLVPLRRQEMGFSYLMVMITITLMGLAMTMAARQWKTMVQR
                     DLEADLLAKGMEIQTALALYSTSAKAGRVVRGEVYPQTLAELTRPPKPFLRKVYLDPM
                     GRGEWELLRAPTGGIMGVRSKSRHKPIKQGNFPLAVRHFEGKPTHYDWVFQYPNPSMV
                     AAAGPVVRSSAIPALPAIYDQPIVPEEPSSPDQSMVSDEMDSPRDAMNMTDSPPSVPP
                     TSPQEPAANPPPPEVTP"
     gene            1836..3146
                     /locus_tag="VN647_01920"
     CDS             1836..3146
                     /locus_tag="VN647_01920"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWG95814.1"
                     /translation="MEALDKTASDQNIDESKPVPVLEYVLELVSKAKQASRRLASLPT
                     ATKDQALLAMAKALEAKSDELLAANEQDVKAFETTPVKKAMADRLRLTEKRIAEMAAG
                     IREVAKLPDPVGTMSAMWTRPNGMKVGRVRVPIGVIGIIYESRPNVTADSAALCLKSG
                     NVCVLRGGSEAIQSNTTIAAVLSEASEEAGVPPGAITFVDRADREVVPVLLKRDRFID
                     LIIPRGGESLMKLIAEHSTIPVVKHDAGVCHIYVDADADAAMAEAICVNAKAQRPSTC
                     NAMETLLVHQSVARVLLPKLARSLSAANVEIRGCPKTRQLISEAKPASEQDYGKEFLD
                     LILAVKIVKNMDEAMEHIAQYGSQHTEAIVTSDYGRAMRFLKEVDAGAVLVNASTRLN
                     DGYQFGLGAEIGISTSRIHARGPMGLDELTCSKFIVLGSGQLRE"
     gene            3147..>4503
                     /locus_tag="VN647_01925"
     CDS             3147..>4503
                     /locus_tag="VN647_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95815.1"
                     /translation="MPSDTIAFALNTPGHLRFPSTRTLAESFDKVGGHVETFPNGHLV
                     FYRPDGRRFLETDHVGHPLHECEWESTGVGTVFLRRARVRLDWGRWIGIKPCGLVNET
                     RLNLASRSNWQGITPNDLRAMAAGALRVPIEEVRWFYRDEDLAINPKGMATIRQRKDT
                     LYVLDGGGFETMRFMACMGAMHWDQIDFLPVVELFKSLLPGTGSAVFELIRGLYDDQA
                     KGYSAPRALRYRGIPTYPSEAAFRLFSGFFTPQPLDGEDPFTAFMNPSKSHLVVWLPA
                     QYPPVRYFDESQGLCLTVKNGIAQKATLAEDPAGLPYVSSIGRRVLPLDRSLHVEGSL
                     LILRDRETELTLPLNHDLMVRPSPSGECPISPVDWRSVFVPGIPRISPAEAFEAVPLF
                     PEDDTEISELAAQSFVADYLDDLGEQDREVGRLRSQAERVFIANGDAVISTCILFDRP
                     RD"
BASE COUNT          990 a         1157 c         1329 g         1027 t
ORIGIN      
        1 cgagaagcaa gaaaagcgaa aatggatctg agccgagtat gggcggtgct tcccgccgag
       61 cgcgacttta ctccgttagc actcggaatt tcagacgaag caaagcgtgc tcaagttacc
      121 ttgccggcat tgtcctataa gacggagcct actctcgtcg cgaatacgag caaaggattg
      181 ttgcaggggc cgatgaccgg gcgatatgag gatctccgcc gattcatcta tggtctcgag
      241 acggcggaag agttgctgtt tatagaagat cttgagctga ctcgggccgg aggcgcgcgg
      301 gacgcgctgt tgacattcaa tattaggatt gcaacgtacc ttcgcggcga tactggggag
      361 tctggtctgc caagactagc gcagtagcca tggatgcaaa aagtaagacg attcttgctg
      421 cctcgcttat ctttctgtgg gcaggactgg ctgtgtggca atggcggctg ctgcaggagc
      481 ctgttcgtat tccgctcacg aatgttactg gtcatgcgcc cgtggggcga caggtagaga
      541 cgggaagagc tggtttgcac gtaaatcttg atttgcttgc atcaacgggt cttcagcgtc
      601 aggcgacttt cacagtgcct cgaaacattt ttgccgtgcc ccgctccgac ggcacattcg
      661 cagccgagaa tgccacagct ttcgtgagtc agccttcagc ttcagccaag accatggtcc
      721 aacaagagga tgcgttggaa ttagggcagt atcgatacct tggatttctt cgcatgggcg
      781 agaagcgagg gaacaataat gatattgcgg tactcaggaa agacgatgag gtgctggtgc
      841 tcagggtcgg tgatcgtgtc gatgaccatc tgatcctcaa ggcgatcaat tctgaaagcg
      901 tgactatccg aaacaccggt actcgtagcg atcaatggat tccgttgtcg gaggaatcat
      961 cggaagaacc aatggctcag gagtgatgga taatgtggag cctcttggta ccgctgaggc
     1021 ggcaagaaat ggggttctca tacctcatgg tgatgattac catcactctt atggggcttg
     1081 ccatgacgat ggctgctcgg cagtggaaga cgatggtgca acgcgacctc gaagcagatt
     1141 tgctggccaa ggggatggaa attcaaacgg ccctggcgct ctactcgacc agcgcaaagg
     1201 ccggaagggt ggtacgtggt gaggtgtatc cgcaaacact cgccgaactc acgagaccgc
     1261 ccaagccctt tctgaggaag gtgtatctcg atccgatggg acgcggtgag tgggaattgc
     1321 tgcgggcgcc caccggtggc attatgggag tccgaagcaa gagcagacac aaaccgatca
     1381 agcaagggaa ttttccgctt gcagtgcgtc atttcgaggg aaagccgacc cactacgatt
     1441 gggtattcca atatccgaac ccgtccatgg tcgctgcagc tggccctgtg gtcaggtcgt
     1501 ctgctattcc tgcgctgccc gccatatatg accagccgat agttccagag gaaccgagtt
     1561 caccggatca atcgatggtc tcggatgaga tggattctcc acgcgacgca atgaatatga
     1621 ctgactcgcc tccctcggta ccccccacct cgccacaaga acctgctgcg aatcctcctc
     1681 caccggaagt aacaccgtag gtcatgcaag gtcgcattga ccattgccta cacttcgcgc
     1741 tgccacggag tgctatggcc agactcttga cccgtctctt actcacacgg tattgtcaac
     1801 gtcgttcccg agttgttcaa ttattggtgt aaagcgtgga agctctcgac aagactgcct
     1861 cagatcagaa catcgacgag tccaagccgg tgcctgtttt ggagtatgtt ttggagctgg
     1921 tttccaaggc caaacaggcg tcgaggagat tggcgtctct gccgacagcg accaaggatc
     1981 aagcactcct tgccatggcg aaggcactcg aggcgaagtc ggacgagctc ctcgcagcga
     2041 atgagcaaga tgtgaaggca tttgaaacta cccctgtgaa gaaggcgatg gcagatcgct
     2101 tgcgattgac tgagaagcgg atcgctgaaa tggccgccgg cattcgcgaa gtggcaaagt
     2161 tgcccgatcc tgtaggaacg atgtccgcta tgtggacgag gcccaacggg atgaaggtcg
     2221 ggcgggtccg tgtaccgatt ggagtgatcg gaatcattta tgagtcgcgt cccaatgtga
     2281 cggcggattc agcggccctc tgtcttaaat caggcaacgt ctgtgtgttg cgaggcggca
     2341 gcgaggcgat tcagtccaat acgacgattg ccgctgtttt gtcggaggca tcggaagaag
     2401 ccggtgttcc acccggcgcg atcacattcg tcgaccgtgc agatcgtgag gtggtgccgg
     2461 tgttgctcaa gcgggatcgg ttcatcgact tgatcattcc gcgtggtggg gaatcgctga
     2521 tgaaactcat cgcggaacac tcgacgattc cggtcgtgaa gcatgatgca ggcgtatgcc
     2581 atatctatgt cgatgccgac gcggatgcgg cgatggcgga ggccatttgt gtcaacgcca
     2641 aagcgcagcg accgtcaact tgcaacgcaa tggaaaccct gctggtccac cagtcggtcg
     2701 cgcgagttct gttgcccaag cttgcccgaa gcctcagcgc agccaacgta gagattcgtg
     2761 gttgtccaaa gacccgccaa ttgatctctg aggccaagcc ggcaagcgaa caagattacg
     2821 gcaaagaatt tcttgacctg attctggccg tgaaaatcgt gaagaatatg gatgaagcga
     2881 tggaacacat cgcgcagtat ggatcgcagc acacggaagc catcgtgacg tcggattacg
     2941 gacgcgcgat gcggtttctc aaagaggtcg atgccggggc cgtgcttgtg aatgcctcta
     3001 ctcgactgaa cgacgggtat cagttcggtc tgggagccga aatcggtatc agcacttcgc
     3061 gcattcatgc acggggccct atggggttgg acgagttgac ctgttccaaa ttcattgtct
     3121 tggggagcgg ccaactccgc gagtgaatgc cctcggacac cattgcattt gccctgaaca
     3181 ctcccggcca tctccgattt ccttccacac gtacactcgc tgagtccttc gacaaggtcg
     3241 gcggacacgt tgaaactttt ccaaacgggc acctcgtttt ttatcgacct gatggacgcc
     3301 gctttcttga gacagaccat gtaggccatc ctttgcatga gtgcgaatgg gagtcgaccg
     3361 gcgtcgggac cgtctttctg aggcgagcgc gagtccgact tgattggggg cgatggatcg
     3421 ggattaagcc ctgtgggttg gtgaatgaaa ccaggctgaa tctagcatca cggtccaatt
     3481 ggcaagggat cactccgaat gatcttcgtg ccatggctgc cggggcgtta cgagtaccca
     3541 tcgaggaggt gcggtggttt tatcgcgatg aggatcttgc tattaatccc aaagggatgg
     3601 ccacgattcg acagcgaaaa gacacattgt atgttttgga tggtggggga tttgaaacga
     3661 tgcgcttcat ggcctgcatg ggagccatgc actgggacca gattgacttt ctcccggtgg
     3721 tcgaactgtt caaatcgtta ttgcccggga cgggctctgc cgtgttcgag ctgattcgcg
     3781 gattgtatga tgatcaagcc aagggatact ctgctccgag ggcgttacgg tatcgtggga
     3841 tccctaccta tccatccgag gcagcctttc gcttgttcag cggatttttc acacctcagc
     3901 cgttagatgg tgaggaccca ttcacagctt tcatgaatcc ctcaaaatct catcttgtcg
     3961 tctggctgcc ggcccaatat ccacccgttc ggtattttga cgagagccaa gggttgtgtc
     4021 tgaccgtgaa aaacgggatt gctcaaaagg ccacgcttgc cgaagatccg gccggtcttc
     4081 catacgtcag ttcgattggc cgtcgtgtac tcccgctgga tcgtagcctc catgttgaag
     4141 gaagtctgtt gattttgaga gatcgtgaaa cggaattgac cttgccgttg aatcacgatc
     4201 tcatggtgag gccgtcgccg tctggcgaat gtccgatcag tccggtcgat tggcgctcgg
     4261 tattcgtgcc aggaattccg aggatttcgc ctgccgaggc cttcgaggcg gtgcccttgt
     4321 ttcccgaaga cgacacggag atcagcgagc tcgctgccca atccttcgtc gcggactatc
     4381 tcgacgatct tggggaacag gatcgagagg ttggacggct acgatcgcag gcagagcgtg
     4441 tgttcattgc gaacggggat gccgtcattt ccacttgtat tctctttgac cggccacgtg
     4501 act
//
LOCUS       DATNDD010000087         2951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_638964, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000087 DATNDD010000000
VERSION     DATNDD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638964"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /gene="truB"
                     /locus_tag="VN647_01930"
     CDS             complement(<1..765)
                     /gene="truB"
                     /locus_tag="VN647_01930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWG95816.1"
                     /translation="MDRTGVTTDQGGALEGVLIVHKEAGWTSHDVVAKVRSLLRGSKI
                     GHAGTLDPSATGVLPILVGRATRIAEYLIDWDKEYRAVLRVGETTDTQDATGRILSRV
                     DACEVTEDALQSAITRFRGVQRQLPPMYSAVKVGGQPLYKAARAGKTVEREERSIAIH
                     QLEILAVHGRDVILRIVCSKGTYIRTLCADIGGALGVGGHLLALERRRVGPLSIEQAM
                     TIDQVLGHLTAGTLRERFISLDQLLFQLPAVVVNDEQ"
     gene            complement(788..1180)
                     /gene="rbfA"
                     /locus_tag="VN647_01935"
     CDS             complement(788..1180)
                     /gene="rbfA"
                     /locus_tag="VN647_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWG95817.1"
                     /translation="MSKTTYKRADRVADQIRMEVADILMRKIKDPRVHDVTVTDVELT
                     GDLRIAHVFVTTMETGEAERNIFAGLSKASGFVRSELGRRLSLRYLPDVIFKKDVSGA
                     RGDRVMRLLEELHGESEERENQGSPNNG"
     gene            complement(1223..1567)
                     /locus_tag="VN647_01940"
     CDS             complement(1223..1567)
                     /locus_tag="VN647_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWG95818.1"
                     /translation="MEASEFSGNRYHEPTLTESPTMVVGLCTVELFVPGSQSLKDKRQ
                     VIHGLKDRLRGKFNLSVAEVEGQDLWQKAVLGMACVSNENKHVNQVLDQALNLIKSMP
                     AVEVVRTQLELL"
     gene            complement(1567..>2951)
                     /gene="infB"
                     /locus_tag="VN647_01945"
     CDS             complement(1567..>2951)
                     /gene="infB"
                     /locus_tag="VN647_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWG95819.1"
                     /translation="YTVSVRGKQVTFLDTPGHEAFTAMRARGAKVTDVVILVVAADDG
                     VMPQTTEAIHHAKAAGVPLLVAMNKVDKPTANPDRVKNALSEHGLISEAWGGDTIMVE
                     VSAKQKTGLDTLLEMILLQAEVLELKADPHRQAKGTVIEAKIERGRGPVATVLVQSGT
                     LRVGDAYVVGTFSGRVRALINDRGEKAQQAGPSIPVEVIGLPGVPSAGDVFHVVSNER
                     VAREIAEERAQKRRAAELTGPAKVSLDDLFAKIQEGSVKELAIVIKADVQGSSEALAG
                     AVEKLSTEAVKLRVIHNGVGGVMESDVLLAAASRAIIIGFNIRPEPKATALAEQQGVD
                     VRLYTIIYDAIADIKAAMEGLLEPTLKERVLGRAEVRQVFTIPKVGPVAGSYVIDGTI
                     SRASAGVRVIRDNVVVYQGKLASLRRFKDDVREVQQGYECGLSIENFNDVKAGDIIEA
                     YAIDKIAAKL"
BASE COUNT          613 a          915 c          740 g          683 t
ORIGIN      
        1 ctgctcatca ttcacaacca ccgctgggag ttggaagagg agttggtcca acgaaatgaa
       61 ccgttcacgg agcgttcccg ccgtgagatg gccgagaact tgatcgatcg tcatggcctg
      121 ttcgatcgac aatgggccga cacggcgacg ttcgagagca aggagatgtc cgccgacgcc
      181 taacgcccct ccgatgtcgg cgcacaatgt gcgtatatac gtgccttttg aacacacgat
      241 gcgcaggatc acgtcacgac cgtgaacggc cagaatctcc agctgatgaa tcgcgatcga
      301 tcgctcttcc cgttcgaccg ttttgcctgc tctggcagct ttgtagagcg gttgtccacc
      361 cactttcacg gctgagtaca tcggtggcag ttgacgttgt actcctcgga atcgagtgat
      421 cgcggactga agcgcgtcct cagtcacctc acacgcatcg accctgctca aaatccgccc
      481 ggttgcatcc tgagtgtcgg tcgtctctcc cacacgtaag acggcacggt actccttatc
      541 ccaatcgatc aggtattcgg cgattctcgt ggcccgcccg acaaggatgg gcaagacgcc
      601 cgttgcactc gggtccaatg tgccggcatg accgattttg ctcccgcgca ataagctccg
      661 aaccttggcc acaacatcgt gtgaagtcca accggcctcc ttgtggacaa tgaggactcc
      721 ttctagagcg cctccttggt cggttgtgac gcctgtgcga tccataccct taagagcctg
      781 cgatccccta cccattgttt ggggagccct ggttctcacg ctcttccgat tccccatgca
      841 attcttccaa cagccgcatg acacggtcgc cacgagctcc actgacatcc ttcttgaaaa
      901 tcacatccgg aaggtagcgg agcgagagcc gccgtcctaa ttccgaccgc acaaacccac
      961 tggcttttga gagaccggca aagatattcc gctcggcctc gcccgtttcc atagtcgtga
     1021 caaaaacatg agcgatgcgc aaatcccctg tgagctcgac atccgtgacc gtcacgtcat
     1081 gaactcgggg atccttgatt tttcgcatga gaatatccgc cacttccatc cggatctggt
     1141 cggccacacg gtctgctctt ttataggtcg tcttcgacat cctgttattc cgacgttgtc
     1201 aatcgccagc attgcggggc gcctagagca attccaattg ggttcggacg acttcgaccg
     1261 ccggcatgct tttgatcaga ttcaacgcct ggtcaagcac ctgattcacg tgtttgtttt
     1321 cattcgaaac acaagccatt ccaaggacgg ccttctgcca gaggtcctga ccctccactt
     1381 ctgcgactga gagattaaac ttaccccgaa gtctgtcctt gagcccatga atgacctgtc
     1441 gcttgtcttt aagcgattga ctccctggga cgaataattc caccgtgcat agccccacga
     1501 ccatggtcgg ggattcagtt agagtaggct catgataacg gttgcctgaa aattctgagg
     1561 cttccactag agcttcgctg caatcttatc gattgcatag gcctcgatga tatcgccggc
     1621 tttgacatca ttgaagttct cgatgctcaa gccgcattca tacccctgct gcacttcacg
     1681 cacatcatcc ttgaaacgcc gcaaggaagc gagcttgccc tgatagacca cgacattgtc
     1741 gcgaatgacg cgaactccgg cgcttgcccg ggagatcgtt ccgtcaataa cataagaccc
     1801 tgctacaggc ccgaccttcg gaatcgtaaa gacctgccga acctccgctc gtcccaatac
     1861 gcgctctttc agggtaggct cgagcaagcc ttccatcgca gccttgatgt cggcaatggc
     1921 gtcataaatg atcgtgtaaa gcctgacatc gacaccttgc tgttcggcca atgcggttgc
     1981 cttcggttcc ggccggatat tgaagccgat aatgatggcc cgggatgcgg cggcaagcag
     2041 cacatcggat tccatgactc caccgactcc gttatgaatc acacgaagct tgacggcttc
     2101 agtcgacagc ttttcgactg cgcctgccaa cgcttcggac gatccttgta cgtcggcctt
     2161 gatgacgatg gccaattcct tcacagatcc ttcttggatc ttcgcgaaga gatcatccaa
     2221 ggacactttc gcaggaccgg tcagttctgc cgcccgccgc ttttgagccc gctcttccgc
     2281 gatctctcgg gcgactctct cattggagac gacatgaaag acgtctcccg ccgacggaac
     2341 gcccggcaat ccgatcactt ccaccggaat ggacggtcct gcctgttgcg ctttttcgcc
     2401 acgatcatta atgagggccc ggacacgtcc gctgaatgtt cccacgacat acgcgtcacc
     2461 cactcgtagg gtcccgctct gaaccaacac tgtcgccaca gggcccctcc cccgctcgat
     2521 cttggcttca atgacggtgc ctttggcctg ccggtgcgga tccgccttga gttcaagcac
     2581 ttcagcctga agcaaaatca tctcaaggag agtatcgagt cccgttttct gtttggcgga
     2641 tacctcgacc ataatggtat ccccgcccca tgcttcggag atcagcccat gttcagaaag
     2701 ggcgtttttg acccgatcag gattggcggt cggcttatcg accttattca tcgccacgag
     2761 cagcggcact ccggccgcct tggcgtggtg gatcgcttcg gtggtttgcg gcatcacgcc
     2821 gtcgtctgct gcgacaacca agataacgac atccgtgacc ttggccccac gagctcgcat
     2881 ggccgtaaag gcttcatggc cgggagtgtc cagaaacgtc acttgtttac cgcgtaccga
     2941 gacggtatag g
//
LOCUS       DATNDD010000088         5765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3488655, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000088 DATNDD010000000
VERSION     DATNDD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3488655"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VN647_01950"
     CDS             complement(<1..255)
                     /locus_tag="VN647_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95820.1"
                     /translation="MRIVLYFIFVIVLGVSAVTINGSSAHAKTVESGDSSSSWIGTGE
                     FLELGNGEQVVNGIVKGVLISRHKDGGKMIVHSSKLACPVR"
     gene            631..1224
                     /locus_tag="VN647_01955"
     CDS             631..1224
                     /locus_tag="VN647_01955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWG95821.1"
                     /translation="MEFRRATREDIPAILDVQAANFIGNLEEVERQDGFLSVKFTSKQ
                     LEEMMGHVGIIVAAIGGRLAGYLCASSCEFNRPFPLLAAMMRQFDGIDYRGRSLASSR
                     AFIYGPVCIDRSYRGRGLLRGLYDTLQQEVVGRYDIGVAFVADDNPHSLRAHVDGLGM
                     ARVGGFVFSGKPYHILAFHVRSENGNGAASKPSAEDA"
     gene            1221..1811
                     /locus_tag="VN647_01960"
     CDS             1221..1811
                     /locus_tag="VN647_01960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWG95822.1"
                     /translation="MSEQRQELWLIRHGETEWSAAKRHTGRTDIPLTPAGERKATALG
                     RFLAGRRFGLVLCSPLRRASETCRLAGYGPVAEVTDDLLEWDYGVYEGRTTEEIRLEQ
                     PEWSIWTASVTKGESIEQVAHRARRVIQRALAVDGDVVLFAHAHILRILTACWLGLPP
                     DAGRWFMLRTASISVLGFERETRVITRWNLLQEEHI"
     gene            1808..2254
                     /locus_tag="VN647_01965"
     CDS             1808..2254
                     /locus_tag="VN647_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWG95823.1"
                     /translation="MKAHYLGHVVFYVKSLDRSLGFYRDLLGFKEVGRVFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPKGSRRGLYHIGIKVGDNLDELRRAKQELEQAGVSIDGM
                     SDHTVSQSLYLKDPDGNEVELYVDADESVWKNNPAAVLSPIKPLQL"
     gene            2351..3025
                     /locus_tag="VN647_01970"
     CDS             2351..3025
                     /locus_tag="VN647_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95824.1"
                     /translation="MALTVDDIKKWLGSLEQKRDELRLQAHLLGMEARDEFDKVDRSI
                     DKFRGAVQWVSSIASVAAREAPATIKTGAEKLTRTVSDSITEFQTAHADTLDTLKQRA
                     GDLHAEIEKATLSLQDAVGSLKDKADETSADFRRRAEEAKTEFAAKAEDAKTQIAGKL
                     EEMKHKGQEMKERSELLSEGVKGALKGMAEDFSRHLGELHTDLDKRLTALKGKVESSG
                     RSEAKK"
     gene            3246..4001
                     /locus_tag="VN647_01975"
     CDS             3246..4001
                     /locus_tag="VN647_01975"
                     /inference="COORDINATES: protein motif:HMM:NF009385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG95825.1"
                     /translation="MNNHTLKGKVAVIGAGAKNLGGLLSREFAKGGAKIVVHYNSDAT
                     KPNADDTVKAIKDVGGDAYAVQSDLTKPANVTALFKSAKDRFGGIDIAINTVGKVLRK
                     PIVDTTEAEFDSMFDINAKAAYFFIKEAGKHLNNNGKIITIVTSLLAAFTDGYSTYAG
                     GKAPVEHFTRAAAKEFGQRGISVTAVGPGPMDTPFFYGQETPERVAFHKSQGMNGRLT
                     KIEDIVPIVRFLATEGWWVTGQTIFANGGYTTR"
     gene            4098..4868
                     /locus_tag="VN647_01980"
     CDS             4098..4868
                     /locus_tag="VN647_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWG95826.1"
                     /translation="MTRSTSGRQWTPPREKYDGKAYRSSNKVLVIDVGGTNVKILATG
                     KRTPRKISSGPSMTARDMVEAVRKLAADWSYDAVALGYPGAVRDGRPAAEPKNLGRGW
                     IGFNFEKTFDCPVKVINDAAMQALGSYDGGRMLFLGLGTGLGSALITDGVLVPMELAH
                     LRYRKGRTYEDYVGLRGLKRFGKKKWRRYVMDIVTALKNALQADHVVLGGGNAKRLKR
                     LPKDVRLGDNGHAFTGGFRLWEEAWKDARTACVVHWGR"
     gene            5175..>5765
                     /locus_tag="VN647_01985"
     CDS             5175..>5765
                     /locus_tag="VN647_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95827.1"
                     /translation="MRKMMILGSMVLSLSMPASYALADQGGTHGHGKGTHMKVTGTVS
                     AIQSDLITVKTSWGQIRISSATAPKNLEVGEEVEMQVNENNVVVDVHRAGDPSHSHRF
                     VSGKLAYASTDRKEIKLWTPEGKKKFDVQTGRSQLSGIKEGTPVAVELNEAGKVIDIH
                     RMTVEIELDEHPHTLSGYHLTLDGVVTKIQSGLAFVK"
BASE COUNT         1368 a         1514 c         1737 g         1146 t
ORIGIN      
        1 acgcaccgga caggccagtt tcgatgaatg gacgatcatc ttgcctccgt ccttatggcg
       61 agaaatcagc acccctttca caattccatt gacgacctgt tctccatttc caagttccaa
      121 gaactcgccg gttccgatcc aactcgaact ggagtctcct gactcaacgg tcttggcatg
      181 cgctgaagag ccgttgatcg ttactgcgga cacacctaag acaatcacaa aaatgaaata
      241 aagaacgatt cgcatgcgat ctcctttcag ttacgatcag ctcggaaatg acggtggtgc
      301 ggagtttcag tctgggtgtc gtacgcacgt atctataata cgaacgagtc ttgttgagca
      361 agtgcagctc tttcacgaac gtagacatta gcgtcaattt gtcgcgagga ccagccttgg
      421 cgacagccgg ggacttgatg gcgagattgt cggagcattc gttgccgttc ggcctcctct
      481 tgaatttcgt catcgggaga tggcagggtc acaatcaccc cattgtgcgc ttgaaccgtt
      541 caagccgtta tgtctataac ctctggaaga gcaggacacc cctcacgttg aacgcagaca
      601 ctgcacgctc gtcatgatcg ggtaagagcc atggaattca gacgggcaac gcgtgaggac
      661 atcccggcca ttctcgatgt gcaggcggcg aacttcatcg gcaaccttga agaggtggaa
      721 cgccaggatg gctttttgtc ggtgaagttt acatctaaac aactcgagga gatgatgggc
      781 catgtcggga tcatcgtagc cgccatcggt ggccgcctgg ccggttacct ctgcgcctcc
      841 tcctgtgaat tcaatcgacc attccccctg cttgccgcca tgatgcggca gttcgacgga
      901 atcgactatc gcggccggtc gcttgcgtcc tctcgtgcat tcatttatgg accggtgtgc
      961 atcgatcggt cttaccgcgg ccggggactt ctccgcggac tgtacgacac actgcaacag
     1021 gaagtcgtcg ggcggtatga catcggggta gccttcgtcg ccgacgataa tccgcattcc
     1081 cttcgcgccc atgtggatgg actcggaatg gctcgagtcg gaggatttgt cttctccggg
     1141 aagccgtatc acattctggc gtttcatgtg agatccgaaa atggaaatgg agcggcgagc
     1201 aagccatccg ccgaagatgc atgagcgagc agcgccaaga gctctggttg attcgacatg
     1261 gtgaaacgga atggagcgcg gccaagcggc atacgggccg gaccgatatc cccctgacgc
     1321 cggcgggcga gcgcaaggct accgcgttag ggcggtttct ggccggtcgg cgatttggat
     1381 tggtactctg tagcccgttg cgccgggcga gcgagacctg ccgtctggcc gggtatggac
     1441 cggttgccga agtgaccgat gacctgctcg aatgggacta tggtgtctat gaaggaagaa
     1501 cgacggagga aatcaggttg gagcaacctg agtggtcgat ctggacggct tcagtcacca
     1561 agggcgagtc gattgagcag gtcgcgcacc gcgcccgacg ggtgatccag cgggcgctgg
     1621 cggtcgacgg cgacgtggtg ctgtttgcac atgcccatat cctgagaata ctaaccgcgt
     1681 gctggctcgg tctaccgccc gatgcggggc ggtggttcat gctccgcacc gcctcgataa
     1741 gcgtgctcgg cttcgagcgt gaaacgcgcg tcatcactcg gtggaacctc ctacaggagg
     1801 agcatatatg aaagcacatt atttgggtca tgttgttttc tacgtgaaaa gcctagaccg
     1861 atcgcttggt ttctaccgag atctgttggg cttcaaggaa gtcgggcggg tcttcaacgg
     1921 agcagccgcg gccctgacat cgggccgaac gcatcacgaa ttgttgttga tccaagtcgg
     1981 cgatgctccg ggaccaccga agggatctcg ccgagggctg tatcatatcg gcattaaggt
     2041 gggagacaac ttggatgaac tccgacgcgc caagcaggag cttgaacagg ccggcgtctc
     2101 gatcgacggc atgagtgatc atacggtgag ccagagtctc tatctcaaag atcccgacgg
     2161 caatgaagtg gagctctacg tcgatgcgga tgagtcggta tggaaaaaca atccggcagc
     2221 ggtgttgtcg ccgatcaagc cgctgcagtt atagcgtggt acagatatgg gacaaaaggg
     2281 acacaattga gcacgctctg caatgcctca ccgtgggctc atgaatcgcg ctgaaaagga
     2341 ggacaggaac atggcgttga cggtcgatga tatcaagaaa tggctgggtt ccttggaaca
     2401 gaagcgggat gagctgcggc tgcaggccca cctgttgggg atggaggccc gcgacgaatt
     2461 cgacaaagtg gaccgatcga tcgacaagtt tcgaggtgcg gtgcaatggg tgtcgtcaat
     2521 cgcatcggtc gcggctcggg aagcacccgc tacgatcaag accggtgccg agaaactcac
     2581 taggaccgtc tcggattcca tcaccgaatt tcagactgcg cacgccgaca ccctcgatac
     2641 cctcaaacaa cgggccggcg atcttcacgc ggagatcgaa aaggccaccc tgtcgctcca
     2701 ggatgcggtc ggatcgttga aagacaaggc ggacgagacg tcggccgatt ttcggcgccg
     2761 agccgaggaa gccaagacgg aattcgccgc aaaagccgag gacgccaaaa cgcaaattgc
     2821 agggaagctt gaggagatga agcacaaagg ccaggagatg aaggaacggt cggaactcct
     2881 gtcggaagga gtgaaaggag cattgaaggg catggcggaa gacttttcac gtcatctcgg
     2941 cgaactccat accgatttgg acaagcggct gaccgcactc aagggcaagg tcgaatcgtc
     3001 cgggcggtcc gaagccaaga aataggatgc cgcatcgggt caagaacata gctgatcaag
     3061 agaaagcgtg gcggtggcag atgaaatggc gcgcgctgtg tcgggccatg atctcgcggt
     3121 cagttgttca atccagatgt ttacaatctt caacatgggc tattaaacac aatatggaag
     3181 ctcatctgaa cgcgagcacg ttctgactcg ttcctgaacc gaccatcaat ggaaggagca
     3241 acatcatgaa caatcatacc ctgaaaggga aagtcgctgt cattggggcc ggcgccaaga
     3301 atttgggcgg cctcctcagc cgcgagttcg ccaagggcgg tgccaagatc gtggtgcact
     3361 acaacagcga cgccaccaag ccgaatgccg acgatacggt gaaagccatc aaagacgtcg
     3421 gtggtgatgc ctatgccgtc cagagcgact tgaccaagcc tgccaatgtc actgcgttgt
     3481 tcaaatccgc caaggaccgc ttcggcggta tcgacatcgc catcaatacc gtcggcaagg
     3541 tgctgcgcaa acccatcgtc gacaccaccg aggcagagtt cgactcgatg ttcgacatca
     3601 acgccaaagc agcgtatttc ttcatcaagg aagccggcaa acatctcaac aacaacggga
     3661 agatcattac catcgtgacc tcgctgctcg cggccttcac cgacggctac tcgacttacg
     3721 cgggcggcaa ggccccggta gaacatttca cccgcgcagc agccaaggag ttcggtcaac
     3781 gtggtatttc cgtgactgcg gtcggtcccg gcccgatgga cacgccgttt ttctatggcc
     3841 aggaaacgcc tgaacgggtg gcttttcaca aatcgcaagg gatgaacggc cggctgacca
     3901 aaatcgaaga tatcgtgccg atcgtcaggt tccttgctac cgagggttgg tgggtcacag
     3961 gtcaaacgat attcgccaac ggcggataca ccactcggta acgacgatag agcatccaac
     4021 agccggcgtt gcagtcagca gagcagcctt caatttcgat cgtgcgttcg gcagcaggac
     4081 ggaaatgaga tgacgacatg accagaagca catcaggacg acaatggaca ccgccacgag
     4141 aaaaatacga cggcaaggcg tatcggagtt ccaacaaggt gctggtcatc gacgtgggcg
     4201 ggaccaacgt aaaaatcctt gccaccggta aacggacgcc acgcaaaatc tcttccggtc
     4261 caagcatgac ggcgcgggac atggtcgaag cagttcgaaa acttgccgcc gattggtcct
     4321 atgatgccgt cgcactgggc tatccaggcg cagtacggga cggccggccg gctgcagaac
     4381 ccaagaactt ggggagggga tggataggat ttaattttga aaagaccttt gattgtccgg
     4441 tcaaagtgat caacgatgcc gcgatgcagg ctctcggcag ctatgacggc ggtcgcatgc
     4501 tgttcttggg gctcggcacc ggtctgggct ccgcattgat caccgatgga gtgctggtgc
     4561 cgatggaact tgcgcacttg cggtatcgga aaggtcgcac gtacgaggac tatgtcggct
     4621 tgcgcgggct caaacggttc ggcaagaaga aatggcgtcg atatgtcatg gatatcgtaa
     4681 cggcgctgaa gaatgcgctt caggccgatc acgtcgtgct gggaggcggc aatgccaagc
     4741 ggctcaagcg tctgccgaaa gacgtgcgcc tcggcgacaa cgggcatgcc ttcaccggcg
     4801 ggtttcgcct ctgggaggag gcttggaagg atgcacgcac agcctgtgtg gtgcactggg
     4861 gtcgttagaa ggcgccataa tatgtatgtg gcggaggaga ggagcagaac gtcctgaagg
     4921 gggcgttgct tcgccccgat ttatcggttt atgaggtgag agagttgcgt ttccccttaa
     4981 agatctagat acttcctact ccaaaaagtg aaatatctcg aggtttcctc tggtaaggat
     5041 cttccggaag ccggagtggt gggtctatgt atcaatggag tcgaaatctt caacctacaa
     5101 ccaaggaggg tactgtgagc ggtgaaattc aacggtcacc ggaatgggtg ggatacatga
     5161 gaaggagaac gcacatgcgc aagatgatga ttctcggttc gatggtcctg agcttgtcga
     5221 tgccggcgtc ctatgccttg gccgaccaag gaggaacgca cggacatggc aagggaaccc
     5281 acatgaaggt cacaggcaca gtgtcggcca ttcaatcgga cctcatcacc gtgaagacgt
     5341 cgtgggggca gatcaggatt tcctccgcta ccgcgccgaa aaacttggag gtcggggaag
     5401 aagtggagat gcaggtcaac gagaacaatg tcgtggtcga tgtccatcgt gcaggagacc
     5461 cgtcccattc ccatcgcttc gtctccggca agttggccta tgcgtcaacg gataggaagg
     5521 agatcaagct ctggacaccg gagggtaaaa agaagtttga tgtgcaaacc ggccggtccc
     5581 aactctcagg cattaaggaa ggcaccccgg tcgccgtcga actgaacgaa gccggtaaag
     5641 tcatcgatat ccatcgcatg acggtggaga tagaactgga cgaacatccc cacaccctat
     5701 cgggctacca tctgacgttg gacggcgtcg tgacgaaaat tcagtccggt ctggcgtttg
     5761 tgaag
//
LOCUS       DATNDD010000089         6171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4118647, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000089 DATNDD010000000
VERSION     DATNDD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4118647"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VN647_01990"
     CDS             complement(<1..195)
                     /locus_tag="VN647_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95828.1"
                     /translation="MSEEVLHRHRAVWQQKPVLRRLYENWYRDIVAWLMQGRTVEIGG
                     GTGNLKSHVPGIYCSDVMVLP"
     gene            complement(195..1709)
                     /locus_tag="VN647_01995"
     CDS             complement(195..1709)
                     /locus_tag="VN647_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG95829.1"
                     /translation="MESTRVYQLPSSPTSLEPVSVEAVDPFQTRSQSQADVKKLAVRD
                     HFDAYAGQRPFWEQKAGAYYEDQARYFRFLVPEGLHVLEVGSGLGTLLAAVKPARGVG
                     IDLSSEMVREASRRHPMLEFRVGDVETLELNETFDVIILADLVGHLVDVEAAFGRLRQ
                     ACKPHTRIIVSYHNYLWEPVLRLCEKVGMKMPQPEQSWLSPTDIANLLRLADFDMVKV
                     ERRLLLPKSIPFLSSVCNNLLAYLPGFRALCLSHYVVARPRLRRPERPYSTTIVIPCR
                     NERGNIDAALRRIPRFGGRQQIIFVDGHSSDGTPEEIRRLMACYPGKDIKLLVQDGNG
                     KGDAVRKGFAHATGDVLMILDADLTTPPEALSKFYEALASGKGEFINGCRLVYPMESQ
                     AMRVLNLIGNKFFGLAFSWLLNQRIKDTLCGTKVLFRREYERIAANRHYFGDFDPFGD
                     FDLLFGASKLNLHIVEIPIRYQARAYGSSNIQRFSHGWLLLKMTVYGFFRLKAV"
     gene            complement(2053..2562)
                     /locus_tag="VN647_02000"
     CDS             complement(2053..2562)
                     /locus_tag="VN647_02000"
                     /inference="COORDINATES: protein motif:HMM:NF021584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HWG95830.1"
                     /translation="MQRLVVLSVVLLAFLISPAAFPQSFTDKAKKDSAVDMSDEDPAM
                     QKAMERARTGLEDFLRKAGSPPTNTDQYSVKVRVSEGEKQEYLWVSNLKAQGDLWSGR
                     IDNLPIIRSVKKGQSYSFAKTEIVDWTYIDRSKKKVFGNFTTCALLTKEPPGVAEAIQ
                     KQYGLECDR"
     gene            2801..3607
                     /locus_tag="VN647_02005"
     CDS             2801..3607
                     /locus_tag="VN647_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWG95831.1"
                     /translation="MARFAHRRWQNLCLFLRAVGRTPPATRLGISAVLLFIVLLGINW
                     AYHTFYKPTELFFPVENALTKSPRETWREYGALFEVHSTAIITPELLAALAQAEGSGN
                     PVARTYWRWRFVSSNPLEWYQPASTAVGMLQITDGTFQEGKRYCIHNHVVVEDGPWHN
                     FNSCWFNSLYTRVIPGHAIELTAAYLDRHVAKAVGGRPATFQQKQDLASVIHLCGAGA
                     GRDYAKRNFRLLANQHCGDHDVKAYLTRVRAMQRSFAMLAAGSIPTSIHR"
     gene            3697..5181
                     /locus_tag="VN647_02010"
     CDS             3697..5181
                     /locus_tag="VN647_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HWG95832.1"
                     /translation="MDRLLSVALALTLLTPSIGSADGSILQSKESQTGELIGPTLPDQ
                     ETHGHHTTFNSGSTVLNWETGAGRSYLIPAGELLAYLFLLNQYDRHFTDPKSAYRTDG
                     DSIWQHLTDSKWVLDNDQFSVNQFLHPYGGSIYYGIARSTGLSFWESWLYSSAGSFFW
                     EIAGETTPPSINDMIATPIGGTLLGEPLFRMASLLLETDEGKPGFWRELGAAVLSPPM
                     GFNRLIFGDRFDTVFPSRQPATFLRLRLGGIVSTSSHNVPSGVREHGAIGDVTFTYGL
                     PGKPGYRYARPFDYFDFHVTAVTTNTLESINTRGLLMGTTYALGEPTRGAWGLFGSYD
                     YISPQVFRVSSVALSLGTIWQSWLSRSIALQGVALAGPGYGAAGSIQRVEERDYHYGT
                     TGQGLLALRLIFGDRAMLDFTGREYYVSGLLSAEPHGQENILRGETSLTFRILGPHGV
                     ALRYAVSHRDARYPNVEFRDQTVETISLMYVFLGKTGFGAVEWR"
     gene            5273..>6171
                     /locus_tag="VN647_02015"
     CDS             5273..>6171
                     /locus_tag="VN647_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG95833.1"
                     /translation="MGSSGPKALWKTVLAGAIGNVLEWYDFALFGYFAPVLSHLFFPA
                     SDPSLSLIATFAVFAVGFLARPLGALLFGYWGDTRGRRAALAWSIILMALPTCLVGLL
                     PTYAQIGLVAPLALTALRFLQGLSVGGEFTGSVTFLVEHAAPTERGYIGSWAGFSAQI
                     GALLGSGIGTLTTVSLSSEALQEWGWRMPFVAGSVIALVGWYLRRRIPESPDFERLQQ
                     AGVVSSSPVRELFTSHRAPLFQVIGLVLLHGVGFYIFYVFLPTYLTKVTDLPMGTALL
                     INTMCMALLAMLIPFMGKLSDRVG"
BASE COUNT         1291 a         1759 c         1669 g         1452 t
ORIGIN      
        1 tggtaatacc atcacatcgc tacaatagat tcccggcacg tgggatttta gattgcccgt
       61 gcctcccccg atttcgaccg tgcgcccttg catcagccaa gcgacgatgt cccgatacca
      121 gttttcatac aaccgtcgaa gcacaggttt ctgctgccac acggctcgat gccgatgcaa
      181 gacttcctcg gacatcacac ggccttcagg cgaaagaatc catagaccgt catcttcagc
      241 aataaccacc cgtgcgaaaa acgctgaata ttggagctgc cgtatgctcg ggcctgatac
      301 cggatcggaa tttccacaat atgcagattc agtttcgatg ctccgaacaa caggtcgaaa
      361 tctccgaacg ggtcgaagtc gccgaagtag tgacgattgg cggcgatccg ttcatactcg
      421 cgtcggaaca agaccttggt cccacagagg gtgtccttga tcctctggtt caagagccaa
      481 gagaatgcca ggccgaaaaa cttgttgccg atcagattga gtacacgcat ggcctgcgac
      541 tccatgggat agacgaggcg acaaccgttg atgaactcgc cctttccgct cgcaagagcc
      601 tcgtagaatt tcgaaagggc ttccggcggc gtcgtcagat ccgcatccag gatcatgagc
      661 acgtcaccgg tggcatgtgc aaagccttta cgcaccgcgt cgcctttccc attgccgtct
      721 tgcaccagaa gcttgatatc tttgcccgga tagcaggcca tcaggcgtcg aatctcttcg
      781 ggcgtcccat cgctcgaatg cccgtctaca aaaatgatct gttggcggcc gccgaatcgt
      841 ggaatccgtc tgagcgccgc gtcgatattt cctcgttcat tccgacaagg aatgacgatc
      901 gtcgtcgagt atggacgctc cggccttcgg aggcgcggtc gtgccactac atagtgactc
      961 aggcacagtg cgcggaaacc cggaagatag gcgaggaggt tgttgcatac agaagaaaga
     1021 aacggaatgg atttgggcaa taacaaccgc cgttctacct tgaccatatc gaaatcggcc
     1081 aagcgtaaga ggttggcgat gtccgtcggc gagagccaac tctgttcagg ttgcggcatt
     1141 ttcatgccga ctttttcgca gagccgaaga accggctccc acagatagtt atggtaggac
     1201 acaatgattc gtgtatgcgg cttacaggct tggcgtagcc gcccgaaggc agcctcgaca
     1261 tcaaccaaat ggccgaccag gtcggcaaga ataatcacgt cgaacgtttc gttaagctcc
     1321 agcgtttcta cgtccccgac tcgaaactcc agcatgggat ggcgccgaga tgcctccctg
     1381 accatttccg aactcaagtc gatgccgacc ccacgagcgg gcttcacggc ggcgagcaga
     1441 gtgcccaagc ccgaaccaac ctctaacacg tgcaagcctt cgggtacgag gaatctgaaa
     1501 taacgagcct gatcctcata gtaagccccg gctttctgct cccagaaggg acgttgaccg
     1561 gcataggcat caaagtgatc gcgaacggcc agtttcttta catccgcctg ggactgactc
     1621 ctcgtttgaa atggatcgac agcctcgaca gaaaccggct ccaaggaggt cggagaagat
     1681 ggcagttgat agactcgagt cgattccatc gaaagatcca tgcgagagat accgtgcacc
     1741 gtaccactag atcgacataa ccgcacgtga tttccaatac ttacgcacgt attcgaagga
     1801 ttagatacac taggggactg caggcgtaag gcccgatcag gtcgcagtat gtataagtga
     1861 agcggtaacc ccggctcagg gcattatcag gcgacttgta gagatcgcga cgcggcgacc
     1921 agccgtcttc agaggaattg caaaggagtt tgagtatgtt gctgattatc gtcaccttct
     1981 ttgtttgtat cggtttaata atcctcgttt ctaccttatt catagacaac aactaagaga
     2041 aacggtaagg cctcaacgat cgcattcgag gccatattgc ttttgaatcg cttctgcgac
     2101 acccggcggc tccttggtga ggagcgcaca tgtggtgaaa ttgccgaaaa ccttcttctt
     2161 actcctatcg atatacgtcc aatcgacgat ctccgtcttg gcgaatgagt atgactgtcc
     2221 cttcttgact gaacggatta tcgggaggtt gtcgatccgt ccggaccaga gatctccttg
     2281 tgctttcagg ttggagaccc acagatattc ttgcttctca ccttcactca ctctgacctt
     2341 gaccgaatac tgatcggtgt tggtcggcgg cgatccggct tttcgaagaa aatcttccaa
     2401 gcccgtccgg gcacgttcca tggctttctg catcgcaggg tcctcgtcac tcatgtcgac
     2461 ggcgctgtcc ttcttggcct tgtcggtgaa tgattgcggg aacgcggcgg gggatattag
     2521 gaacgccaac aggacgacgc tcagcacgac gagccgttgc ataccaacct cccagagtct
     2581 ctcctgctct ccgtgaatga tgtgagtatt gtgacttggc catattgagc ctgtccaggc
     2641 cgcaatgcaa gatcgttcac acattcagcg tctcacagaa cccttgcgtc catccatcac
     2701 ataatctcag gaagaattta tttgttttct gttactctcc aacatgcgcc gtcgccgcca
     2761 tacccgttcc cgctctcgtc atcctcatcc agtgatgtcg atggcgcgct tcgctcatcg
     2821 gcgttggcag aatctctgct tgtttctccg ggccgtcggc cgaacaccac ccgcgacgag
     2881 gctgggaatc agtgccgtcc ttctcttcat cgtcctcttg ggtatcaatt gggcgtatca
     2941 cacgttctac aagccgacgg aattgttctt tcccgtggag aatgcgctca ccaagagtcc
     3001 acgagagaca tggcgagaat acggcgcgtt gttcgaggta cattcgacag ccattattac
     3061 acccgaactg ctcgccgcgt tggcccaagc tgaaggctcc ggaaatccag tggcacgaac
     3121 ctactggcgc tggcgtttcg tctcgtcgaa tcccctagag tggtaccaac cggcctcgac
     3181 ggcagtcggc atgctccaaa tcaccgacgg cacgtttcaa gaagggaaac gctattgcat
     3241 ccacaaccat gtggtcgtcg aggacgggcc gtggcacaac tttaactcct gttggttcaa
     3301 cagtctctat accagagtta tcccaggcca cgcgatcgaa ctgaccgctg cgtatctcga
     3361 ccgtcacgta gcgaaggcgg tcggaggacg accggcgaca ttccaacaga aacaggactt
     3421 ggcctcggtc atccacctct gcggcgccgg ggcgggccgt gactacgcaa aacgtaactt
     3481 tcgtctcctg gccaaccagc actgcggtga ccatgatgtg aaagcctatc tcacgagagt
     3541 cagggccatg caacgtagct ttgcgatgtt ggcggccggg tcgattccca ccagtatcca
     3601 tcgttgagcc cgaccaggat ggaaccaaga cgaccttcag ccctaggggg gtgccgagta
     3661 ttaatagaac ctagtgtatt atccactcgt agacgcatgg atcgtttatt atcagtcgct
     3721 ctggcattga cactcctgac gccaagtatt ggttcggctg atggctccat cttgcaatcc
     3781 aaggaaagcc aaacaggtga actgatcggt ccgacccttc ccgatcaaga gacgcatggc
     3841 catcacacga ctttcaacag tggaagcacg gtcctcaatt gggaaaccgg cgccggtcgc
     3901 agttatctta ttcccgcggg cgagcttttg gcctacctct tcctcctcaa tcaatacgac
     3961 cgacatttta ctgaccccaa gagcgcctat cgcacggacg gcgattccat ttggcaacat
     4021 ctcacggatt ccaaatgggt tctcgacaat gatcagtttt ccgtcaatca atttctgcat
     4081 ccgtacggcg gcagcattta ttatggaatc gctcgatcga caggcctcag tttctgggaa
     4141 tcgtggctct atagttcggc cgggagtttc ttttgggaga tcgcggggga aaccacaccg
     4201 ccatcgatca acgacatgat cgccactcct attggtggca ccttgctggg agaaccgctc
     4261 tttcgcatgg cgagtctgtt gctcgaaacc gacgagggaa aacccggctt ctggcgcgaa
     4321 ctgggtgcgg cggtgctttc acctcctatg ggattcaacc gtctgatctt cggtgaccgc
     4381 ttcgatacgg tcttcccgag ccgccaaccg gccacgttct tgcgtctgcg actcggcggc
     4441 atcgtctcca cgagcagtca caacgttcca tcgggcgttc gcgagcacgg cgctattgga
     4501 gatgtcacat ttacgtacgg gttgcccggt aagccgggct atcgctatgc ccgtccgttc
     4561 gactattttg actttcatgt cacggcggtc acgaccaata cgctggagag catcaatact
     4621 cggggcttgt tgatgggaac cacctatgcg cttggagagc cgacccgtgg agcgtggggt
     4681 ctgttcggca gttacgatta tatttcaccg caagtattcc gggtctccag cgtcgcgctt
     4741 tcgctcggca ccatctggca atcctggctt tcgcgttcga tcgctcttca aggcgtggcg
     4801 cttgccggtc ccggctatgg cgcagccggc agcattcagc gtgtcgaaga acgcgactac
     4861 cactatggaa cgacgggtca agggctcctc gcgctgcggc tgattttcgg ggatcgtgcg
     4921 atgctcgact tcaccggacg tgaatactat gtcagcggtc ttctgtccgc ggagccgcat
     4981 gggcaagaaa acatcctgcg aggtgaaacg tccttgacgt tccggattct cggcccccac
     5041 ggcgtggcgc ttcgctatgc cgtctcgcac cgcgatgccc gctatcccaa tgtcgaattt
     5101 cgagatcaga cggtcgaaac catcagcctc atgtatgtgt tcctcggtaa gacaggtttc
     5161 ggcgcggtgg agtggcgctg agtgagatgg tacaaggcac agccaggaca ctccggcgtc
     5221 cattcatggt atggtggccg caactttgcg ctgttgtatg aggggcggct tcatgggttc
     5281 aagcggaccg aaagcattat ggaagacggt cttggccggc gcgatcggga atgtgctgga
     5341 gtggtatgac tttgccctct ttggatactt cgccccggtc ttgtctcatc tcttcttccc
     5401 tgcttcggat ccgtcattat cattaattgc cacgttcgcc gtcttcgccg tcggctttct
     5461 tgcgagacca ttgggtgcgc tgctgttcgg atattggggt gataccaggg ggcgacgagc
     5521 ggccttggcc tggtccatta tcttgatggc attgccaacc tgcttggtcg gcctactgcc
     5581 gacctatgct cagatcggtc ttgttgcacc gctcgcactc acggctctgc gttttcttca
     5641 aggtttatcg gtcggtggag aattcaccgg ctccgtgacc ttcctcgttg agcatgccgc
     5701 accgactgag cgaggctaca tcggtagttg ggccgggttc agcgcacaga tcggggcgct
     5761 gttgggttcc ggtatcggca ccttgacgac tgtgagcctc tcatcggagg cacttcagga
     5821 atgggggtgg cgcatgccgt tcgtggcggg aagtgtcatc gcgttggtcg ggtggtatct
     5881 tcggcggcgc attcccgagt cgccggattt cgaacggctg caacaagccg gtgtggtctc
     5941 ttcctcgccg gtccgtgagt tattcacatc gcaccgagcg ccgctatttc aggtgattgg
     6001 tctcgtcttg ttacatggcg tcgggttcta catcttctat gtgttcctcc ctacttatct
     6061 gacaaaagtg accgacctcc cgatgggaac ggcactgctg atcaacacga tgtgtatggc
     6121 tctgttggcg atgctgatcc cgttcatggg caagctgtcc gatcgagtcg g
//
LOCUS       DATNDD010000090         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2404468, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000090 DATNDD010000000
VERSION     DATNDD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404468"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..294
                     /locus_tag="VN647_02020"
     CDS             37..294
                     /locus_tag="VN647_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWG95834.1"
                     /translation="MSIIGWILFGLVVGVVAKLLMPGRDPGGMIVTILLGIVGALIGG
                     FIGRFLGWYGENDPVGFIMAVIGAVLVLFAYRKISGPSPVR"
     gene            471..797
                     /locus_tag="VN647_02025"
     CDS             471..797
                     /locus_tag="VN647_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWG95835.1"
                     /translation="MADNRGCSLAAVGWAFVSGGLLGAAAALLLAPQSGRQSQEQLRG
                     YARRAEESVHELADKATEVVDQALDKGREFIQEKKSVLTEAVEAGRTAMHRERERLSG
                     GKNACR"
     gene            847..1023
                     /locus_tag="VN647_02030"
     CDS             847..1023
                     /locus_tag="VN647_02030"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HWG95836.1"
                     /translation="MNTIVIVVMGVVLLSVLPLWPYSANWGYLPSGGLGFLLLVLVVL
                     TLLGRLGSSAAEKS"
     gene            1227..1412
                     /locus_tag="VN647_02035"
     CDS             1227..1412
                     /locus_tag="VN647_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWG95837.1"
                     /translation="MIDINSGTFDQLKTLVGIGDTYAKRIVEGRPYQRMDELLTKKII
                     PQTTYDKIKDHILMKPK"
     gene            1498..1806
                     /locus_tag="VN647_02040"
     CDS             1498..1806
                     /locus_tag="VN647_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95838.1"
                     /translation="MKNRSRTSIMIVTAFFMGFGLGGLSLHYAAAQGIFGLSTSVSQL
                     GSALMDMQKNVDDLQKNMGTVKQVKDQLSSLSPQGGGTPGGDLLKKGGDSLKDMKPKF
                     "
     gene            1865..2545
                     /locus_tag="VN647_02045"
     CDS             1865..2545
                     /locus_tag="VN647_02045"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWG95839.1"
                     /translation="MNRISSSAASTGFISSETRLGMIWGVMAGMVVLLIGNPVASAEQ
                     TATESVKSTIDEVIHILNSEELKQPGRSVERRQKIEQVIRQRVSYEDMAKRALGWPWI
                     ELTNSERQEFVALFVQLLRDTFACRIDNYADEQVLYLSEQREENVAEVKAKLSGLKVD
                     TLLDFRLADKVGHWYVYDVIIDGAGIVGNYRAQFASIIRDHSYAGLVNKMKEKTLVVK
                     AFETTTDP"
BASE COUNT          657 a          621 c          862 g          597 t
ORIGIN      
        1 gaaatggtat ggcctgaagc acatgggaga taaatcatgt cgattatagg ctggattcta
       61 tttggattgg tcgttggcgt ggtcgcgaag ctattgatgc ctggacgcga tccgggagga
      121 atgatcgtga cgatcctctt gggtatcgta ggtgcgctca tcggtggttt tatcggtcgc
      181 ttcctcggat ggtatgggga aaacgatccg gtcggtttta tcatggccgt gatcggggca
      241 gtcttggtgt tgttcgccta tcgaaaaatc agcggccctt cgccggtgcg ataggcttat
      301 tggggacttg tatgctcgcg gatcaggacg tcgccgaaca acgggaaagt atgccgccat
      361 ccatagaccc acacgaattc gggagggccg ctgatcggcg caggttccgg tgtcatcaag
      421 ccacatattc acgggacaga gggatggtca atggaaaagg aggaggccta atggcagata
      481 acagagggtg ttcgttggcg gcggtgggat gggcttttgt cagcggagga ttgttgggag
      541 ctgcggcggc gttgttgctg gcaccgcagt ctggtcgtca gtcgcaggag cagttgcgag
      601 ggtatgcgcg acgagccgaa gagagtgttc atgagctggc cgacaaagct accgaggttg
      661 tggaccaggc attggacaag ggacgcgagt tcatccagga gaagaagtct gtcctgaccg
      721 aggcggtcga ggccgggcgt acggccatgc acagagagcg tgagcgactg tccggcggca
      781 agaatgcctg ccggtgaagc gcttgggtac agaatgatcg aatgtgcgaa caaagggaga
      841 aaccagatga atactattgt gatcgtcgta atgggggtgg tgcttctttc cgtacttccg
      901 ctttggccgt acagcgccaa ctggggatat ctccccagtg gcgggctggg tttcctgctg
      961 ctggttctgg tggtcctgac cctgctcggt cggctgggat cgtcggcagc tgaaaagtcg
     1021 taatggagtg agcggtcgta gataaatacg cctctgttgg aggatctact tttggaggat
     1081 ctacttttgc gctgatgtca agttccgtca gggcgttacc ctgtcttgaa cgggggtgct
     1141 tgcgcaggtt ctcagggagg tgagtagcca tgctctcggc ctcgggatcg tgcatatggc
     1201 ctattccaag gatatagcac gggaccatga tcgacattaa ttcagggaca ttcgaccagc
     1261 tcaaaactct ggtcggaatc ggagacacct atgccaagag gatcgtggag ggacgaccct
     1321 atcaacgcat ggatgagctg ctgacgaaga aaattattcc gcaaacgacc tacgacaaga
     1381 ttaaggatca cattctaatg aagccgaaat aatgcaaccg acgacaaatc aggaagcgac
     1441 gaatcggcca gtggtcaaag cacaggtagc cggcaacgaa gcgtaaagga ggggaatatg
     1501 aagaacagat ctcggacatc catcatgatc gtgacggcat ttttcatggg atttgggctg
     1561 ggaggcttat cactgcacta tgctgccgcc caagggatat ttgggctttc gacgtcggtg
     1621 agccagctcg gcagtgcgtt gatggacatg cagaaaaatg tcgacgatct tcaaaagaat
     1681 atgggaacgg tcaaacaggt caaagatcaa ctgagcagtc tgtccccaca aggtgggggc
     1741 actccaggag gagacctact gaaaaaaggt ggcgattcat tgaaagacat gaagccaaaa
     1801 ttctgagtcg catgacacga gcacatgggt gacagcgtca tctattactg tggggaggga
     1861 acccatgaac cggatatcca gcagtgccgc tagtacggga ttcatcagtt ccgaaacgcg
     1921 tctcggcatg atctgggggg tgatggccgg tatggttgtg ctgctgatcg ggaatcccgt
     1981 ggcttcagct gagcagaccg ccacggaatc agtcaagagc actatcgatg aggtgatcca
     2041 tattctcaac agtgaagagc tgaagcagcc gggccgttcg gtggaacgac gtcagaagat
     2101 tgagcaagtc atcaggcagc gtgttagtta tgaagacatg gccaaacggg ccctgggctg
     2161 gccctggata gaactgacta acagcgagcg gcaggaattt gttgccctct ttgtacagtt
     2221 actccgagac acgtttgcct gtcgaattga caactacgcc gacgagcaag tgctctactt
     2281 gtctgaacag cgggaagaga acgttgccga ggtgaaagcg aagctgtcgg gccttaaggt
     2341 cgataccctc ttagattttc gtctagccga caaggtcggg cattggtatg tgtacgatgt
     2401 gatcatcgat ggcgcgggga tcgtaggcaa ttaccgtgcg cagttcgcca gcattatccg
     2461 cgaccattcc tacgcaggtt tggtgaacaa gatgaaagag aaaacacttg tggtgaaagc
     2521 gtttgaaacg accacggatc cataagttgg tgtacttcag caactgagtc agcctggcga
     2581 ctcccaagtc ggtcggaaag cgcatgaata cgatgttttc acctgtcgcg aagagtgccg
     2641 agctggaaga accgattcgc gccgagcttt tcggcgtcga gcggctcgaa cagcatgctg
     2701 caagcctcgc ggtggcacag gtcgtcaccg aagatgc
//
LOCUS       DATNDD010000091         7548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3829080, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000091 DATNDD010000000
VERSION     DATNDD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829080"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1890
                     /locus_tag="VN647_02050"
     CDS             193..1890
                     /locus_tag="VN647_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95840.1"
                     /translation="MNKNLLFGSLLLSGLLMAFEVTLPDFVTAASKAPQKATGNKAAI
                     DTALRYAEAIAKGDKITTGQLDFVCQYRLLTALSAGLPNPRTTDASYEECWQELIAGH
                     APVLKRSDMGMNILWPSTGSLVFYGDDLPRTQASAFVMDVLGVSPPGTGLHLTVTDSR
                     PIPNGSFPLKPNGKVLGVPTTLVDLTVHYHDPLSSPVTYGPGTVHWTNTIKRERRAIK
                     SITTRWVVFTGLKKYGFPRDSAVCHLPVKAKPDEAPGMVADRIPFTTETSRALPDSIV
                     WWGPDDQPGTLTAAAAQAAAFPELRDRVAMLNRILIIDSHQVDALTVLTRNLYTVLLR
                     EAVNGHNLTVKNPALSLTVNEFYWNVYAAAGRLDLANTMEMGGFSQPTSADFLYRLLP
                     ALETLAKTHPERLDTRFQLGMAYRWNNDQIPMIETFEALVKDIPTDRPTPKAEALVQL
                     AWSRINKVAWNRRLHDPNSVRAYEDARDSSAIAELPLDKFFAEYTMAYSMIFMPNYGD
                     KNRMLGHLTEAKRWFDEIPGKDDEVWRYFLHSELLKAVLDADPMFQPILASAQQPKG"
     gene            1963..2643
                     /gene="aat"
                     /locus_tag="VN647_02055"
     CDS             1963..2643
                     /gene="aat"
                     /locus_tag="VN647_02055"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWG95841.1"
                     /translation="MNRHELRFPPVDRASPEGLLAVGGDLCPERLLEAYRHGIFPWYN
                     DDQPILWWSPDPRAVLFPNKLHVSHSLKRRLRPDVFTVTLDTGFRSIMEQCAGPRPQY
                     PQGGTWITGDMLDAYIRLHELGYAHSVETWQDGRLVGGLYGVAIGGAFFAESMFTRVD
                     DASKVALVRLVKQLQAWNFRLIDCQQFSPHVSRFGAEEIPRSDFIKHLNQALTLPDRR
                     GRWTFETV"
     gene            2754..3074
                     /locus_tag="VN647_02060"
     CDS             2754..3074
                     /locus_tag="VN647_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWG95842.1"
                     /translation="MSFAITPTELKARLDKGDNLVLLDVREPWENQLAKLDNSILIPL
                     GMLPNSLSKLDRNAEIIAYCHHGMRSGDATGFLLQQGFSNVKNLIGGIDAWSVQVDGA
                     VPRY"
     gene            complement(3164..4087)
                     /locus_tag="VN647_02065"
     CDS             complement(3164..4087)
                     /locus_tag="VN647_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG95843.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLVEEGHQVVIVDNLATGKRKNVNRA
                     ASLYKTDITSWRLERVFRNERPNIVLHLAAQISVRRSVDDPVFDAQVNVLGTMNVLQQ
                     AVRYGCRKVVFSSSGGAIYGEQETFPAAESHASNPLSPYGISKLCGEHYLFYFQRTSG
                     IPVVSLRYANVYGPRQDPEGEAGVIAIFIQKMLNNEQPIINGNGRQTRDFVFVDDVAE
                     ANLAAIGQTAHGVYNVGTGTETSVNELFRMLAGLTASSAKEVHGPAKAGEQIRSVIDP
                     SRIKQELGWEVKVDLAEGLKQTVEFFQGKIR"
     gene            complement(4127..4855)
                     /locus_tag="VN647_02070"
     CDS             complement(4127..4855)
                     /locus_tag="VN647_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWG95844.1"
                     /translation="MLTVQGLAQSRDAAVRMILAGKVKSNGVIVDKPSRVIPNDAVIE
                     IIGKNQPFVSRGGEKLAAALDAYSITPRGMICLDVGCSTGGFTDCLLQKGAARIYAVD
                     VGYGQFDWRLRQDPRVVLLERTNIRYMPPSAIPEPVDLVVVDVSFISLIKVLPPITSF
                     LRSPAHVIALIKPQFEVGKGQVGRGGVVRDDTQRAEAAQRVVEFAEEMGLRTMRTVAS
                     PIKGKKGNQEILAIFEYDTSFRGM"
     gene            complement(4951..5208)
                     /gene="xseB"
                     /locus_tag="VN647_02075"
     CDS             complement(4951..5208)
                     /gene="xseB"
                     /locus_tag="VN647_02075"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWG95845.1"
                     /translation="MAGVKFEQAMARLEAIVGELEKGDLPLDESLKIFEEGIRLSKNC
                     LKVLEEAERKVEVLVQDKNGKKQLRAFTSDDIAVPDSAEDS"
     gene            complement(5240..6607)
                     /gene="xseA"
                     /locus_tag="VN647_02080"
     CDS             complement(5240..6607)
                     /gene="xseA"
                     /locus_tag="VN647_02080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWG95846.1"
                     /translation="MTSRQVLTVSELTAMVRVSLETDFAEVWLEGEISNLRAPASGHL
                     YCTLKDQTSQIRAVIFRSAAVRLRFGLEEGLHVVVRGRLSVYEPRGEYQVILDHLEPK
                     GRGALQLAFEQLKCRLEAEGLFDREGKQSLPAFPRIVGIVTSPTGAAVRDLITVLHRR
                     CPILSIIIAPVQVQGPESARQITAAIQAMNKLGVVDVMIIGRGGGSLEDLWSFNEEAV
                     VRAIAASRVPVVSAVGHETDVTLADFAADVRAPTPSAAAEMVAPVLTDLVERLGMLTA
                     RCRQAMSSQCLEQHQRFDLLLAHMDNIRFRVLKEAQRVDGAVAGMREAVRAQLRHAMV
                     NAQAWTQTLMSRSPVIQVRRDLVIVPQLRSRVIAAVNHGLKRKVEHTHAYLGRFNGLS
                     PLAILDRGYGILETMPGHRIIRDAAQVSVGEEILARLAKGRIRCAVEEVKLDPSVSKP
                     AETSL"
     gene            complement(6948..>7548)
                     /locus_tag="VN647_02085"
     CDS             complement(6948..>7548)
                     /locus_tag="VN647_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HWG95847.1"
                     /translation="SVITTGNHAWDKKEAVDYFSREPRLLRPANYPAGVPGNGSVVIE
                     TAGGERLAVLQLMGRVYMPTIDCPFQTAKRELSRLKRETSAIVVDMHAEATSEKMAMG
                     HFLDGDVVAVVGTHTHVQTADEQILPKGTAYITDIGMTGPFHSVIGVKKELAIEKFLT
                     AMPRRFEVASGPSVFCAVLLELDPRLGKAIAFERIRLID"
BASE COUNT         1668 a         2272 c         1964 g         1644 t
ORIGIN      
        1 caagaaatac gccgacatct gggcgattgt ccaaattgtg aaaccttcgt ggcgtcgttg
       61 cgccaaaccg tgtcgctctg ccgccacagc ccggcaccga ctctatcgac ggcggaccgt
      121 gcgttgatgc gtgcgaagat actaaaaacc gcctaatcgg gataacctaa caacaccgtg
      181 agttttttca tcatgaataa aaatctattg ttcggcagtc tactcctctc gggactgctg
      241 atggcctttg aagtcacctt acccgacttt gtgactgcgg cctccaaggc acctcagaag
      301 gcgacaggaa ataaggccgc catcgacaca gcccttcgat atgccgaagc catcgccaaa
      361 ggcgacaaga tcaccactgg acagctggac ttcgtgtgtc aataccgact cctgacggcg
      421 ctgtcggccg gccttccgaa tcctcggaca actgacgcct cgtacgagga gtgttggcag
      481 gaactgattg ccggccacgc cccggtcttg aagcgatctg atatggggat gaatatcctc
      541 tggcccagca ccggttctct ggtattttat ggagatgatc tcccccgtac tcaagcctca
      601 gcctttgtca tggacgtcct cggagtgtcg ccgcccggaa ccggccttca tctcaccgta
      661 acagacagcc gtccgatccc gaacggatcc ttcccactga agccgaacgg gaaagtcctc
      721 ggcgtcccta ccacgctggt cgacctgact gtccattatc atgacccact ctcgtcaccg
      781 gtaacgtacg ggcccggcac cgtccactgg acgaatacga tcaaacgaga gcgacgcgcc
      841 ataaaatcca tcaccactcg atgggtggtc ttcaccgggc tcaaaaaata cggctttccg
      901 cgagactcgg ccgtctgtca tctgcccgtg aaagccaagc ctgatgaagc gccggggatg
      961 gtagcggaca gaattccgtt cacaaccgaa acgagccggg cgcttcctga ctcgattgtc
     1021 tggtgggggc cggatgatca gcccggcacg ctgactgcgg cagcggcaca ggctgctgcg
     1081 tttcctgaat tgcgcgatcg cgtcgccatg cttaatcgca tcctgatcat cgactcgcat
     1141 caggtcgatg cgctgacggt cctgaccaga aatctctata cggtattgct tcgggaagcc
     1201 gtcaacggcc acaatctaac tgttaaaaat ccggcgcttt ccttgaccgt caatgagttt
     1261 tattggaacg tctatgcagc cgccggtcgc ctcgaccttg caaacacgat ggagatggga
     1321 ggcttctcgc agccgacatc ggccgacttc ctgtaccgac tgttgcccgc actggaaacc
     1381 ctggcaaaaa cccatcctga gcggctggat acccgcttcc agctcggcat ggcttatcgc
     1441 tggaacaatg accagattcc gatgatcgag acgttcgaag cgctggtcaa agacattcca
     1501 accgatcggc cgacgcccaa agccgaggcg ctggtgcagt tagcctggtc tcgtatcaac
     1561 aaggtggctt ggaacaggag gttgcacgac ccgaacagtg ttcgagccta cgaggatgcc
     1621 cgcgactcat cagccatcgc cgagctgccg ctggacaagt ttttcgcgga atacaccatg
     1681 gcttacagca tgattttcat gccgaactat ggagataaaa accggatgct cggacatctc
     1741 accgaggcca aacgttggtt tgacgaaata ccaggaaaag atgatgaggt ctggcgctat
     1801 ttcctccata gcgaattact gaaggccgta ctcgatgccg atccgatgtt tcagcccatc
     1861 ctggcttcgg cgcaacaacc taagggataa tggccgatct caagttcatc tcggcatagt
     1921 ctttttgccc tgtttcacgt atcgtacggg gtgatgttcc gcttgaatcg gcacgaactt
     1981 cgttttccac cggttgatcg ggcatcccct gaaggtctcc tcgcggtagg aggcgatctc
     2041 tgtccggagc ggttgctcga agcctatcgg catggcatct ttccctggta caacgacgat
     2101 cagcccatcc tctggtggtc gccggatcct cgcgccgtcc tgtttcctaa caagctccat
     2161 gtctcgcaca gcctcaagcg acggcttcgc cctgacgtgt ttaccgtcac gctcgatacc
     2221 ggctttcgga gtatcatgga acagtgcgca gggcctcgcc cgcagtatcc ccaaggagga
     2281 acgtggatca cgggtgacat gctcgacgcc tatatacgtt tgcacgaact cggctacgct
     2341 cattccgtcg agacatggca ggacggtcgt ctggtcggcg gactctacgg cgtggcgatc
     2401 ggaggggcct tctttgccga atcgatgttt acacgagtcg atgatgcatc caaagtggcg
     2461 ctcgtccggc tcgtcaagca gctccaggcc tggaacttcc gcctcataga ttgccaacaa
     2521 ttctcgcccc atgtcagccg gttcggcgcg gaagagattc cacggtcgga cttcatcaag
     2581 catctcaacc aggccctcac ccttcccgac cgacgggggc gatggacttt cgaaacggtg
     2641 tgagccatag aaggaacaag atcgacgcac cgccacttgg atggcgaagc gcatttgaca
     2701 gccttttctt ccgtcgctta tgattccgtt ttagtcacga ggaggaatat tgtatgagct
     2761 ttgccattac acccacagaa ctgaaggccc gactcgataa aggggacaac ttggtgcttc
     2821 tagatgtgcg agaaccttgg gaaaatcaac tcgccaagct ggacaattcc atcctcattc
     2881 cgctcggcat gctgcccaac tccctttcca aattggacag aaacgccgag attatcgcct
     2941 actgccatca tgggatgcga agcggagacg cgacggggtt tttgttacag caggggtttt
     3001 caaacgtgaa gaacctgatc ggcgggatcg atgcctggtc tgtccaggtt gatggggcag
     3061 ttccccgata ctgatccggt cagataccga tagaggggaa aaggccggcg gttgaatgct
     3121 gaccgcctcg tggttcaagt agagatcggc tttatacgcg cagctacctg attttcccct
     3181 gaaaaaattc gaccgtctgc ttcaaccctt cggcaagatc caccttgacc tcccacccaa
     3241 gctcttgttt gatccttgac gggtcgatca cactgcgaat ctgctcaccg gctttggccg
     3301 ggccatgaac ttccttggca ctcgacgcag tcagaccggc gagcatacgg aacaactcat
     3361 tgacggatgt ttcagtaccg gttccaacgt tgtacactcc gtgtgcagtc tgccctatgg
     3421 cggccaggtt cgcttcagcc acatcgtcga cgaatacgaa gtcgcgcgtt tgacggccgt
     3481 ttccattaat aataggctgc tcgttgttca acatcttttg gatgaagatg gcgataaccc
     3541 ctgcttctcc ctcgggatcc tgtctcggcc catacacgtt ggcataccgg aggctgacca
     3601 ccggaatccc gcttgtccgc tggaagtaga acaagtaatg ttccccgcac agtttactga
     3661 tgccataggg tgatagtggg ttgctggcgt gagattcagc ggcgggaaac gtctcctgtt
     3721 caccgtagat cgctccaccg gacgaggaga acacgacctt tcggcaacca taccgaaccg
     3781 cttgttgtaa cacgttcatg gttcccaaga cgttgacctg cgcatcgaac accgggtcgt
     3841 ctaccgaacg gcggacactg atctgagccg caagatggag gacgatgttg ggccgctcat
     3901 tgcgaaagac ccgttcaagg cgccagctgg taatgtcggt tttgtaaaga ctcgccgcac
     3961 ggttgacgtt cttccgcttt ccggttgcca gattgtcgac gatgacgact tgatgtccct
     4021 cttcaacgag tcggtccacc acatgagacc cgataaaccc tgcgccgccc gtcacgagta
     4081 ctttcattgg ccctcctgaa tgaactctca gcagttacga cattccttac ataccacgaa
     4141 atgaggtgtc gtactcaaag attgctaata tttcctgatt gccttttttc cctttgatcg
     4201 gggaagcaac ggtcctcatt gtccgcagtc ccatttcctc cgcaaactca acgactctct
     4261 gagcagcttc cgctcgctgt gtgtcgtcac gcacgacgcc tccccgaccg acttgcccct
     4321 ttcccacttc aaactgtggt ttgatcaagg cgatgacatg ggccggagaa cgaaggaatg
     4381 acgtgatggg tgggagtacc ttgatcaatg aaataaacga gacatcgacg acgaccagat
     4441 caaccggctc cgggatggca gaaggaggca tatagcgaat gttggtccgc tcaagcagca
     4501 caacgcgtgg gtcttgcctg agccgccaat cgaactgacc atatcccacg tcaacggcgt
     4561 agatcctcgc agcccccttt tggagcaaac agtctgtgaa ccctccggtc gagcacccga
     4621 catcgagaca gatcattcct cgaggtgtga tcgaataggc gtccagagct gccgccagtt
     4681 tttcgccgcc gcgactgacg aacggctggt tctttccgat aatctcgatg accgcatcgt
     4741 tcggaatcac cctggacggc ttgtcgacga tcactccgtt gcttttgact ttacccgcga
     4801 gaatcatccg gaccgcggca tctcgactct gcgccaatcc ctgaaccgtc agcacgtggt
     4861 cgcaacgatc cttaccgcgt cgtatgttcg tacccatgag catagaaggg ggaaagtccg
     4921 gaaaagaacg atgatcagag taacgacttg ttatgagtct tcagcggaat ccggtacagc
     4981 aatgtcatcg gatgtgaagg cacgtaactg tttctttccg ttcttgtcct gaacgagaac
     5041 ttctactttc cgttcggctt cttccaatac tttgagacaa ttcttggaca gccggatgcc
     5101 ttcctcaaaa atctttagcg attcatcgag tggcagatct cccttctcta attcccccac
     5161 gatggcttct agcctagcca ttgcttgttc aaacttcact ccagccacaa caatctcctt
     5221 ttaagcgaca tgcagcccat tataacgaag tctcagccgg ctttgaaacc gacggatcca
     5281 acttgacttc ctctacagca catcggatcc ggcccttggc gagacgcgct aggatctctt
     5341 caccaacaga aacctgtgcg gcatcccgaa tgatccggtg tccgggcatc gtctcgagaa
     5401 tcccatagcc ccgatccaga atagcgagcg ggctcagccc gttgaatcga ccgagataag
     5461 catgggtgtg ctcgaccttt cgcttgaggc catggttcac ggcagcaatc acacgcgacc
     5521 gcaactgtgg aacgatcacc agatcgcgac ggacttgaat caccggactc ctcgacatca
     5581 gcgtttgcgt ccatgcttgc gcattcacca tcgcgtgtct cagctgggcg cgaactgctt
     5641 cccgcatgcc tgccacggcg ccatccaccc gctgagcttc tttgagaacc ctaaacctga
     5701 tattgtccat atgggcgagc agaagatcaa atcgctggtg ctgctccagg cattgcgagc
     5761 tcatagcttg ccgacagcgg gcggtgagca tgcctagccg ctcaacaagg tcagtcaaca
     5821 ctggggcaac catttcggct gcagcggacg gtgttggagc acgaacgtct gccgcaaagt
     5881 cggccagtgt cacatccgtc tcatgtccca ctgccgatac gacggggacc cgtgacgcgg
     5941 cgatcgctcg cacgacggct tcttcgttga agctccacag atcttccaag gaaccaccgc
     6001 cccgcccgat gatcatgaca tcgaccacac caagtttgtt catcgcctga atggcggctg
     6061 tgatctgtct agccgattca ggcccctgca cctgtacagg cgcaatgatg atgctcaaga
     6121 taggacagcg acggtgtaag acagtgatca gatctcgaac cgccgccccg gtcggtgagg
     6181 tcacgattcc gacgatccga gggaacgccg gtaatgattg tttgccctcc ctatcgaaaa
     6241 gcccttcggc ttccagacga cacttcagtt gctcaaacgc cagctggaga gcaccccgac
     6301 ccttgggttc aagatgatcg agaatgacct gatactctcc acgcggctca tacacggaaa
     6361 gtctccctcg cacgacgaca tgcagcccct cctccaggcc gaaccgcaaa cgaacggccg
     6421 ccgaacggaa gatcaccgcc cgaatctggc tcgtctggtc tttgagtgta cagtagagat
     6481 gcccggacgc cggcgcgcgc aggttcgaaa tttccccttc aagccacacc tcggcaaagt
     6541 cggtttcgag ggaaacccga accatggccg ttagttccga gacggtgaga acttgtctcg
     6601 atgtcaactc gaaaggagaa agagaggggt gagggtacgt agtcgtgtgt cttctccttt
     6661 gaagctcgcg agcaggctgt tcaaaaagcc tgtcgttctc acccgcccac cccggcgcgc
     6721 caagacgcgc cgttccacag gccaggccca gcgaagtccg cgacgcgaag aataatgagc
     6781 gtcacgtctg cggacgggcg cgagttggtg agcgcccggt gtcttaaagg caaggcatat
     6841 cctttttcac ccacccgccc cgagctgctc gggcagctct tgcccggtgg tacgttgagg
     6901 ccctgagcga tgcgagaacg ctgctggcgg actttttcaa cagcctgcta atcgatgagg
     6961 cggattcgtt caaacgcgat cgccttgcct aaacgaggat cgagctcaag cagaaccgcg
     7021 caaaacacgg acggtcctga ggcgacttca aaccgacgcg gcatcgcggt caaaaacttt
     7081 tcgatcgcca attctttctt gactccgatc acagaatgga acggccccgt catgccgata
     7141 tccgtgatgt acgcagtgcc cttcggaaga atttgctcat cggcagtctg cacatgcgta
     7201 tgcgtaccca cgacagcgac cacgtcacca tccagaaaat gccccatggc catcttttcg
     7261 gacgtagctt ccgcatgcat gtcgaccacg atcgcggaag tctcccgctt caaccgtgat
     7321 aattcgcgtt tggccgtctg gaacggacaa tcgatcgtgg gcatgtacac ccggcccatc
     7381 agctggagga cggccagccg ctcgccccct gcggtctcaa tgacgacact cccgtttccc
     7441 gggacccccg ccggataatt cgccggacgc aacaagcggg gctcccgaga aaaataatcg
     7501 actgcttctt tcttatccca ggcatggttt ccggtcgtga tgaccgat
//
LOCUS       DATNDD010000092         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_340432, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000092 DATNDD010000000
VERSION     DATNDD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340432"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VN647_02090"
     CDS             complement(<1..859)
                     /locus_tag="VN647_02090"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95848.1"
                     /translation="MKYVMVHAGGMADRPRPELGGRTPLQASSTPNLDRLAQIGELGQ
                     LLTPEDGVRHGSGLIGAAILGYDPRKFYQGPGPLEAASLGVSVTEHDVVYRCTMVTLR
                     PEGRKGAEIKKLGPNVMMDDATAGLIETEDARELIDAINEQLGSETIQFYPGAGHRHL
                     MVWVNGKPRALCTDPQAILGQSIADALPTGDGADILRKLMDAAHLTLRDHPVNDDRVA
                     AGKKPANCIWLWGEGRAVIWPSLVEKYDIGGAVVATSDVYRGVGMNAGLEAVDPERLA
                     DGDFRTKAAV"
     gene            complement(876..1934)
                     /gene="thrC"
                     /locus_tag="VN647_02095"
     CDS             complement(876..1934)
                     /gene="thrC"
                     /locus_tag="VN647_02095"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWG95849.1"
                     /translation="MTRWRGVIEEYQKFLPVTEKTPVISLGEGNTPLIRAARLAKAIA
                     PGVELYLKFEGVNPTGSFKDRGMTLAISKAVESGARAVMCASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHQATVIQIEGNFDQALTLVKDFSTAMHIELVNSVNP
                     FRIEGQKTAAFEVCDQLGDAPTLHVLPVGNAGNITAYWKGYKEYRSANQISRVPRMMG
                     FQAAGAAPIVLGRVVDQPQTIATAIRIGNPASWTSALNAIEESAGAIDMVTDEEILRA
                     YTAVASTEGVFCEPASAASVAGVAKLHRTKILQEGETVVCTLTGHGLKDADTAISVSK
                     QPVTVKATREDVARLLNV"
     gene            complement(1938..>2495)
                     /locus_tag="VN647_02100"
     CDS             complement(1938..>2495)
                     /locus_tag="VN647_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWG95850.1"
                     /translation="AKLVDGEIEARVHPTMLPAASPIAQVEDVYNAIQLVGDAVGDVV
                     LYGRGAGSMPTGSAVVSDVIATSRNLLKGAVGRVPVASFQQDQRKPLRLRSMEEISSL
                     YYLRFTVVDRPGVLAQIAGELGRCGISISSMVQQGRRDGQTVPVVIKTHTAKERDVQT
                     ALREINRRAFVSEPTMLIRVEGKDE"
BASE COUNT          491 a          813 c          638 g          553 t
ORIGIN      
        1 ccaccgccgc cttagtgcgg aaatcgccgt cggccagcct ctcgggatcc accgcttcga
       61 gccccgcgtt cattcctaca ccacgataga cgtcactggt cgccacgacc gcccctccga
      121 tgtcatattt ttccaccaag ctcggccata tgaccgctcg tccttcaccc cacaaccaga
      181 tacagttcgc cggtttcttc cccgctgcaa cccggtcgtc gttcacagga tggtcgcgca
      241 aggtcagatg cgcggcatcc atcaacttcc ggaggatatc cgcaccatct cccgtgggca
      301 atgcatcggc aatggattga ccgaggatcg cctgtggatc ggtacagagg gctcgaggtt
      361 tcccgttcac ccagaccatt agatgacgat gaccggctcc cggataaaac tggatggttt
      421 ccgagccgag ctgttcattg atcgcgtcga tcaattcgcg cgcgtcttcc gtttcgatca
      481 acccggccgt cgcatcatcc atcatcacgt tcggccctaa tttcttgatc tcggcgccct
      541 tccttccctc cggacgcaag gtgaccatcg tacagcgata caccacatcg tgttccgtca
      601 ccgacacacc caaactcgcc gcttccagcg gccccggtcc ctgataaaac tttctcgggt
      661 cgtacccaag aattgcggcc ccgatcaatc cgctcccgtg acgaactccg tcttcgggag
      721 tcagcagttg accgagttct ccgatctgtg cgagacgatc gagattggga gtcgatgacg
      781 cttgcagcgg tgtgcgtccc cccagttccg gcctcggacg gtccgccatc ccgccggcat
      841 gcaccatcac atatttcata agctccacac gtctttcaga cattcaatag ccgagcaacg
      901 tcctcgcgcg tcgccttgac ggtgaccggt tgcttggaca cgctgatcgc cgtatcggca
      961 tctttcaaac catgtcctgt cagcgtgcat acgactgtct ctccttcctg tagaatcttg
     1021 gttcgatgca gcttggcgac gccggcgacg gacgcagctg aagccggctc acaaaaaact
     1081 ccttccgtgg acgctactgc tgtgtaagcc cggagaattt cttcatccgt gaccatatcg
     1141 atcgcgcctg ccgattcctc tatcgcattc agcgccgagg tccagctggc cggattaccg
     1201 attcgaatgg ccgtcgcaat agtctgcggt tgatcgacga ccctgcccaa tacgatcgga
     1261 gcggcaccag cggcttgaaa gcccatcatt cggggcactc ggctgatttg attcgccgat
     1321 cgatactcct tgtatccttt ccaataggct gtaatatttc ccgcatttcc gaccggcagg
     1381 acatgaaggg tcggggcatc gccgagttga tcgcacacct caaaggcggc ggtcttctgc
     1441 ccctcgattc taaacggatt caccgagttc acgagttcaa tgtgcatagc ggtcgaaaag
     1501 tccttcacga gagtcagggc ctgatcaaag ttgccttcga tctgaatcac cgtcgcttga
     1561 tgcatcatcg cctgggagag tttgcccatc gcgatcttac cggccgggat caacacatag
     1621 acagccaacc cggcacgggc tccataggcg gcagccgagg cggacgtgtt tccggtggac
     1681 gcgcacatca cggctcgtgc gccgctctcc acggccttcg agatggccaa cgtcattccg
     1741 cgatctttga acgaaccggt cgggttgacc ccctcaaact tcaggtagag ttcgacccct
     1801 ggtgcaatcg ctttcgccaa cctggcggct cggattagag gagtattccc ctctcccagg
     1861 ctgataaccg gcgtcttctc ggtcacaggg aggaattttt ggtattcttc aataactccg
     1921 cgccaacggg tcatcgatca ctcatccttg ccttcgacgc gaatgagcat ggtcggctcg
     1981 gagacgaacg cacgccggtt gatttcacgt agcgcagttt gcacatcgcg ctctttcgcg
     2041 gtatgtgttt tgatgaccac cggcaccgtc tgcccatcgc ggcgtccttg ttgcaccatt
     2101 gaagaaatgc tgatcccaca gcggcctagc tcacctgcga tctgtgccaa tacgcccggt
     2161 cgatcgacca ccgtaaaccg taagtaatag agcgagctga tctcttccat cgatctcaac
     2221 cggagcggtt ttctctggtc ctgctgaaac gacgccaccg ggactcgacc gacggcgcct
     2281 ttaagcagat tccggctggt cgcgattaca tcgctcacca cagcacttcc ggtgggcatg
     2341 gatcccgccc ctcgaccata cagcaccacg tcaccgacgg catcgccgac gagttggatg
     2401 gcattgtaca catcttcaac ttgggcgatg ggcgacgcgg cgggaagcat ggtgggatgg
     2461 acccgagcct caatttcccc atccacgagc ttcgc
//
LOCUS       DATNDD010000093         4976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1287050, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000093 DATNDD010000000
VERSION     DATNDD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287050"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VN647_02105"
     CDS             complement(<1..467)
                     /locus_tag="VN647_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95851.1"
                     /translation="MMKSGLSLSGALLLGFTSVVAAESEAPTMLPNLFSFPNATGILK
                     TFNSTGKLDLTGPFFQSLGTNGRSCASCHQPGDAWSVSAAHVEDRFERSHGLDPIFRT
                     NDGSNCDHNIDTTTVEGRREAYSLLRSRGLIRIALVVPVGAEFDVVGVKNPYG"
     gene            complement(676..888)
                     /locus_tag="VN647_02110"
     CDS             complement(676..888)
                     /locus_tag="VN647_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95852.1"
                     /translation="MSETGIVDRVRSVLEEHGTACSLDDVTQLCPDLTWNQVFLAVDF
                     LSRTGQADVILDPRRVYWVQTRRTAA"
     gene            1395..1517
                     /locus_tag="VN647_02115"
     CDS             1395..1517
                     /locus_tag="VN647_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95853.1"
                     /translation="MPLIFLCCLVMASAVLSFGLMLWFVVLGIVWFVMPGMKEG"
     gene            1611..2159
                     /locus_tag="VN647_02120"
     CDS             1611..2159
                     /locus_tag="VN647_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWG95854.1"
                     /translation="MFQQAEHQRENGEQAARMPAAVRILLAVDDSDNSRRVVQYVGSL
                     LSRTPDAVITLFHVLKPMPRRFLEHGGSENPAVEAQLSMQLRHEQDAWIQKERESECH
                     VLEQACETLTRSGFDMNRVTVKYGHDDDIARNILEEARSGHYDTIAVGRQGISRIKQL
                     FGGGTTDHLVHAAKGVAIWVVA"
     gene            2599..3018
                     /locus_tag="VN647_02125"
     CDS             2599..3018
                     /locus_tag="VN647_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95855.1"
                     /translation="MNRMPLGIVHIGFACMLAAAGCASDQTARQPTSAASSVSPVTTE
                     AASVGPSDSLKACLAKISSDSNDGVRMIAEQSCQDNEKLHQGVVGTAIAKSGNRASAG
                     TQGDSHDACMARIPTDATSGQRMLAEETCARDQLTHR"
     gene            3055..3420
                     /locus_tag="VN647_02130"
     CDS             3055..3420
                     /locus_tag="VN647_02130"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWG95856.1"
                     /translation="MLPFHWSSGSLMLRARSWRPFYLLWILVAGCAFGAGSGLSDSPS
                     DGAISKEVEIKLSGDQFGGLSEVLVTTEAGVVTLVGTVERAEQKARAADLARQVKGVK
                     RVKNDLEIQRTKASEAPTQ"
     gene            complement(3483..>4976)
                     /locus_tag="VN647_02135"
     CDS             complement(3483..>4976)
                     /locus_tag="VN647_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HWG95857.1"
                     /translation="KAKGAPAGIRFEGRLQKDLLLSVQRCELLLPPFRLTGEGRIRLA
                     NEWEFGAKIRSDALSLDKLPRGVRLGSVSAGIIEAGLKMEGRATDRASWVTSGRLRFD
                     NGVVEGQFQDPIRNLFMRLRFNGKNIDIQRLTFTVGDSDLRLSGSITNWLEAPKVKLV
                     VQSPQIDVASLKPMSPNGSSSSDSSSSVRTWWDNGSLDAMVLIDYLYYGQSLVSGLSC
                     RIRFEHGSLKIDRISGDTDDGHLAGRINLTIPERGPRLVSSMFGISGVPIDHLFSLIE
                     ERPRIRGWVTARGGLQAEFGQDRIFRSSISSRRPISIMIERGRLIYAPVISKVLTLIN
                     LPALLRGKMDLTEDGIPFERLKLVFGVEHGIMNISEFLLDSPILKISGTARYDFIDDK
                     FDGVVVASPLGQYSDLLKSVPLFGRLFSGERHGFDTAIFEVKGSAKDPNVVYLPAESL
                     MAGAKGTAKLAFDLLVNAITLPEEAFSMAEGDSPIEEDAVQEGHRGL"
BASE COUNT         1126 a         1369 c         1422 g         1059 t
ORIGIN      
        1 cacccgtaag gattcttgac gcccaccaca tcgaactcgg ctcctaccgg gactacaagc
       61 gcaatacgga tcaatccgcg gctgcgcaag aggctatagg cttcgcgacg gccttccacc
      121 gtggtggtat caatgttgtg atcgcaattg gagccgtcat tggtccgaaa gatcggatcg
      181 aggccgtgcg accgctcaaa ccggtcctcg acgtgcgccg ccgagactga ccaggcatcg
      241 ccgggctgat gacagctggc gcagctgcgg ccgttggttc ccagactctg aaagaaagga
      301 ccggtcagat cgagtttgcc ggtcgagttg aaggttttca gaatgcctgt ggcattggga
      361 aaggagaaga ggttcggaag catggtcggc gcctcggatt ccgcagccac gaccgatgta
      421 aaacccagca gaagcgcacc cgacaatgag aggccggatt tcatcatagt cgctccgtgt
      481 aaattgtatt aggagtgtgt acgagactgt atggccaaca gacacgacgg cgtggtgacg
      541 acgctggtta tgaatctgtg gaatagacaa aggaagtcac gtgtgcgatg ggcacggagg
      601 tcatgtcaat agcctttctc gaaggaccgg cgaagactgc atcgcagtag cggagactga
      661 gacatcggca tgctcttatg cggctgtgcg acgggtttgc acccaataga cccgtctagg
      721 atccaagatg acatcggctt ggcctgtccg gctcaaaaag tcgacggcta agaacacttg
      781 attccaggtc aagtcgggac agagctgcgt gacgtcgtcc aacgaacagg ctgtgccgtg
      841 ttcttccagc acggaccgga cacgatcaac gatacctgtc tcactcatga gtcacctcgc
      901 ttcctcgccg catatcggca gaagcacatt atgtgccaaa ctgaactttt cattaatctt
      961 ctcaacacac caagcgcacc ggattcataa aatcaagcat gggagtggtt ataagactaa
     1021 gtatgggtac ttaatgaaga ctgtttcagt ttcgtcttga gtttcgtctt gattgtgaac
     1081 tgagcgggat tgccaaaggc gcagagtgcg taatcgctct ttaagaaaac ggatgtataa
     1141 acaaggtgtg gaaataggaa aaactgtggc aacctttcgg ctgaccaaac ccaccaagcc
     1201 gatgcccggt caagagagag ggcaccacca acaccggcct tccttcacct tggatggatt
     1261 ttgatgtcta tgcctgatga ttacctgttt caggccgccg gtcggcttcc gtgcacggta
     1321 tgattttgta actgcttggt cttgatcttg cagcggccac ggggtggctg gattcctgtg
     1381 aggagataac tcgggtgccg cttatcttcc tctgttgcct cgtgatggcc agtgcagtcc
     1441 tttcatttgg gctgatgctg tggttcgtcg tgctgggaat tgtgtggttc gtcatgccgg
     1501 gcatgaagga gggatgaggg aagccgacgg cgttgagcat ctgtatgcgt ggcgaatata
     1561 attatattca tcgatcgtca tgaaacgtct caaggatggg aggggcgccg atgtttcaac
     1621 aggcagagca ccagagagag aatggagagc aggcggcacg gatgccggcc gcggtgcgca
     1681 ttctgctggc cgtggacgac tccgacaact cccgtcgcgt ggtccagtat gtcggctcac
     1741 tcctgagccg aacccccgac gccgtcatca ccctctttca tgtgctgaag cccatgccgc
     1801 ggcggttcct ggagcatggg ggatcggaga atccggcggt cgaggcgcaa ttgagcatgc
     1861 agttgcgcca tgaacaggac gcctggattc agaaggaacg ggaatccgag tgtcatgtcc
     1921 tcgagcaggc ctgtgagacg ttgacccgat cggggttcga catgaaccgg gtgacggtca
     1981 agtatgggca tgacgacgat attgccagaa atatcctgga agaggcccgc agcgggcatt
     2041 acgataccat cgccgtggga cggcagggga tctcgcggat caaacagctg ttcgggggcg
     2101 gcacgaccga ccatctcgtg catgccgcca aaggagttgc gatttgggtg gtggcatgac
     2161 cacgggtggc gctcgtacct tcggcaatct cctcaggaac gctcactttt cgcgcaaaca
     2221 ttcttggcat gaacttctcc ggtttcattg gctggtgact gaggcggacg agttactcgc
     2281 tgaaactgcc tcaatttgaa aagaaactgc tcgtgacctt ggactggacc tggacgtgtt
     2341 gttttcaaag gatccagtcc gctttcgaac gaagcgctcc cgtatcccca catgcggcgg
     2401 cagaattgaa gaaatctgcc tgaatccctc acgtctcgca tcatcaactg ccgaccagac
     2461 ttcggactac cggcaaactg aggacgaagc ctcgcctgga ccttcgatgc ggcttgcgcc
     2521 ctgaagggga ctccgctata tagaaacggg tagaccgcgc tggctcacga ttgccgccgt
     2581 aaaggaggaa tactaattat gaataggatg ccgcttggaa ttgtacacat cggcttcgct
     2641 tgcatgttgg cagcggctgg ttgcgcctcg gatcagaccg cgaggcagcc gacatcagca
     2701 gcctcatccg tgtcgccggt cacgactgag gcagcctccg ttggtccgtc ggattccctg
     2761 aaggcctgcc tcgccaagat ctccagcgat tccaacgatg gggtgaggat gatcgctgaa
     2821 caaagttgtc aagacaatga aaagcttcat caaggcgtgg tcgggacagc cattgcaaaa
     2881 agcggcaatc gtgcatccgc cgggacgcag ggcgattcgc atgatgcctg catggcgcgc
     2941 atccccacag acgcaacgtc cggtcaacga atgctggcag aagaaacctg tgcacgcgat
     3001 cagttgacgc atcggtaaga acaagaagtc gcatctcgat acgtaaggaa caagatgctg
     3061 ccattccatt ggtcttctgg gtcgctgatg cttcgtgcac gatcctggcg cccattctat
     3121 ttgttgtgga ttctggttgc gggctgcgcc tttggtgccg gctcgggtct ttccgattcc
     3181 cccagcgacg gggctatctc caaagaagtg gagatcaagc tctcaggcga tcaattcgga
     3241 gggttgtcgg aggtcctggt cacaaccgaa gcaggcgtcg tgacgctggt gggcacggtt
     3301 gaacgggcgg aacagaaagc cagggctgcg gatctggccc gccaggtcaa aggcgtgaaa
     3361 cgcgtgaaaa atgaccttga aattcagcgg accaaagcaa gcgaggcccc tacacaatag
     3421 ccgcggcacg ggtgcatccc gccttaccgt tcgcttcttc gatagcgccg gcatctcctg
     3481 gtttagagac cccggtgccc ttcctgcacg gcatcctcct ctatgggcga atcaccttcg
     3541 gccatggaaa aggcttcttc gggaagagtg atcgcattaa ccagcagatc gaaagccaat
     3601 ttcgccgttc ccttcgctcc ggccatcaag gattcggccg gcaagtaaac cacgttcgga
     3661 tctttcgccg atcctttgac ctcgaaaatg gcggtatcga atccatgccg ctcaccggaa
     3721 aatagtctcc cgaacaacgg cacgcttttg agaaggtcgg aatattggcc gagcggactc
     3781 gccaccacca ctccatcgaa tttatcgtcg atgaaatcat atcgtgccgt cccgctgatt
     3841 ttcaaaattg gactatccag caggaattcg ctgatgttca tgatgccatg ctcgacgccg
     3901 aacacgagct tcaatcgctc gaacggaatg ccgtcctcgg taagatccat cttgcccctc
     3961 agcaacgccg gcaaattgat gagggtcagc actttggaga tcaccggcgc ataaatgaga
     4021 cgccctcgct cgatcatgat ggaaatcggc cggcggctgg agatggatga acgaaagatg
     4081 cggtcttgtc cgaactccgc ctggagtcct cccctggccg tcacccatcc tctgattcgg
     4141 ggccgttcct ctatgagaga aaagagatgg tcgatcggta cgccgctgat accgaacatg
     4201 ctgctgacca accgcggacc tcgttccgga atagtcagat tgatgcgccc ggcgagatga
     4261 ccgtcatccg tatcgccgct gattcggtcg atcttcaaac ttccgtgctc gaaccgaatc
     4321 cggcatgaca gtccgctcac caaagattgt ccgtaataga gataatcgat caggaccatg
     4381 gcatcgagcg aaccattgtc ccaccaggtt ctgacggagg aggacgaatc ggaagaggag
     4441 gagccgttag ggctcatggg cttcagcgaa gcgacatcga tctgggggga ttgaacgacc
     4501 aactttacct tcggggcctc caaccaattc gtaatggatc ccgacaagcg cagatcgctg
     4561 tcgccgacgg tgaaggtcag ccgctgaatg tcgatattct tgccgttgaa acgcagccgc
     4621 ataaaaagat ttctgatggg gtcttgaaac tgcccttcga ccaccccatt gtcgaagcgt
     4681 aggcggccgc tcgtgaccca tgacgcgcgg tcagtggctc tgccctccat tttgagccct
     4741 gcctcgatga tcccagcgct gaccgagcct agcctcacgc ctcgaggtag cttatccagc
     4801 gaaagcgcgt cagaccgtat tttcgctcca aactcccatt cattcgccag ccggatccgg
     4861 ccttcaccgg tcaacctgaa cggcggaagg agtaattcgc agcgctggac ggacaagagc
     4921 aggtctttct gaagccgacc ttcaaaccgt atcccagccg gcgccccttt ggcttt
//
LOCUS       DATNDD010000094         2721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4143073, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000094 DATNDD010000000
VERSION     DATNDD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4143073"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VN647_02140"
     CDS             complement(<1..215)
                     /locus_tag="VN647_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95858.1"
                     /translation="MPPTLKSDVTYSHNEPPDASQLLGLFHQAPWAKARSLDDAKEML
                     CHTDLTLCAWEGDRLIGFGRVLTDFIYR"
     gene            complement(504..926)
                     /locus_tag="VN647_02145"
     CDS             complement(504..926)
                     /locus_tag="VN647_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95859.1"
                     /translation="MLTTEVLDAVGGLAAHLKSYAAKLPPIVHMSALPSGLKPSSEAV
                     DGYETIVSRVRSQSAGTPYKALNESLVSSLEGFEIGNLLGAVQPLLSALDHLERMQRD
                     KEIEMGRTDEKRLKEYRAALHKVLPGNKPELDGAGAGM"
     gene            complement(920..1924)
                     /locus_tag="VN647_02150"
     CDS             complement(920..1924)
                     /locus_tag="VN647_02150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWG95860.1"
                     /translation="MITEFIVTSGEQPKRLDLFLVNRERDISRSALQRLIELGRIRIN
                     DQVVKASHKIKPGDRITMDVPKPEPLSIKGEAIPLDILVEDDALLVLNKPSGIVVHPA
                     PGNWTGTLVNALLHHFQTSGGTISTIGGKERPGLVHRLDKETSGIMVITKTDAAHRAL
                     AAQFKLHTIIRVYEALVWGVPKKGRGLIDLAIGRDSKDRKKFSPRTTSPKESVTEYVV
                     DRRYGKAAAHVVLYPRTGRTHQLRVHLAALGHPILGDHTYGGRKVCMIEQVEIPRVML
                     HARTLGFTHPATGDVENFTRPVPDDMERVMHALERLIIHREKKLRGPIADMHVAEDAC
                     "
     gene            complement(2027..>2721)
                     /locus_tag="VN647_02155"
     CDS             complement(2027..>2721)
                     /locus_tag="VN647_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95861.1"
                     /translation="LRDALALFSKVPASPAHGDALLTLGKVSAWLGSREEAGRLFERA
                     VDRYNRSRNVQGVVSARIQLGLLRISDGLTQEGLQLLDQALKEAHNRQDHDQTLAALL
                     ALGDARWIMDEPDSAKQYYERALALVEQRPQAGMEAALRYRMSAVYGAIGQQEKGIGS
                     AKRAVTLYQSLRDASGEAASWALLASLYEALGQHQEADEAGQRALAIFRRRQIVVHAA
                     HPSANPLLSESR"
BASE COUNT          564 a          791 c          759 g          607 t
ORIGIN      
        1 cgatagataa agtcggtgag gactcgaccg aacccgataa gacgatcccc ctcccacgca
       61 cagagtgtca ggtcggtatg gcatagcatt tccttcgcat catcgagaga tcgggccttg
      121 gcccatgggg cttggtggaa caacccaagg agctgagacg cgtcaggtgg ctcgttatgg
      181 gagtaggtga cgtcggactt gagggttgga ggcatcttat actagagtat cctcccgaat
      241 tgccgtggag tgtacgggga aacaaatgtc aacgcaagtc cgaacctgtc caaagtgctt
      301 tcaactcatg tggctgaaaa ctgatgagta cgaggtgctc gacgatgaaa ctgttcgcgc
      361 gaagtgccct cactgcggga acaccgcacg ctttaagctc gtcaccgtcg gcccgaatgc
      421 attagggccc aaaatgggac attgaccgct gtgagcggtt gtgagtgctg agctgggtct
      481 ccgaaagcac tcagcactgt cgcttacatg ccggcacccg caccgtccag ctccggctta
      541 ttgcccggaa ggactttgtg cagggcggcg cggtactcct tgagtcgctt ttcgtccgtt
      601 cgtcccatct caatttcctt atcccgctgc attcgttcca aatgatccaa tgccgagaga
      661 agcggttgaa cggcaccgag gaggttcccg atttcaaaac cctccagaga cgacaccaac
      721 gattcattca acgccttata tggcgttccg gcactctggc ttcgaacgcg tgatacgatg
      781 gtttcatagc catcgaccgc ctcggaagaa ggtttcaggc cagagggcag tgcgctcata
      841 tggacaatgg gcggaagttt ggcggcatag gacttgagat gagccgccaa ccctcccacg
      901 gcatccagta cttcggtcgt cagcatgcat cctcggcaac gtgcatatcg gcgattggtc
      961 cacgcagttt cttctcccgg tggatgatga gccgttcaag cgcatgcatc acccgctcca
     1021 tatcgtcggg caccggcctc gtgaagttct ccacatcacc cgtcgccgga tgagtgaatc
     1081 cgagggttct ggcatggagc atgacccgag gaatctccac ttgttcaatc atacacactt
     1141 ttcgcccgcc gtacgtgtga tctcccagaa tgggatgtcc caaggcggcg aggtgaaccc
     1201 gaagctgatg cgttcggccc gttcgagggt agagcaccac atgggccgcc gctttcccat
     1261 accgccggtc caccacatac tctgtaacgg attccttagg gctggtcgtt cttggagaaa
     1321 atttcttccg gtctttgctg tcccggccaa tagccagatc gattagtcct cggcctttct
     1381 tgggaacacc ccagaccagc gcctcataga ctctgatgat ggtatggagc ttgaactgcg
     1441 ctgcaagggc gcgatgcgcc gcatcggttt tagtgatcac catgataccg gacgtttcct
     1501 tgtccagtcg atgaacgagg ccgggccgtt ctttcccccc aatggtcgaa atggttccac
     1561 ccgacgtttg aaaatgatgc agcagcgcgt tgacaagcgt cccggtccag tttcccgggg
     1621 caggatggac gacgatcccg gacggcttat tcaagacgag aagagcgtca tcttcaacaa
     1681 ggatgtccag cggaatcgcc tctcctttga tcgagagcgg ctccggcttc ggcacgtcca
     1741 tggtgatcct atcaccgggt ttgatcttgt gactggcctt cacgacctga tcgtttattc
     1801 gaatgcgacc cagctcgatc aaccgctgca acgccgaccg cgagatgtct cgctctcgat
     1861 tgacaaggaa gagatccagc cgtttgggct gttctcccga cgtcacgatg aactcggtaa
     1921 tcatgggcgg gtacgctact aaagagcatc gaccaaatgc aattggagat atcgttggtt
     1981 tgcttctttc gtaggtctgc ttcggaacgg caatggctct atcgtttcat ctcgactccg
     2041 agaggagggg attcgccgat gggtgggcgg catgcacgac aatctgtcgc cggcggaaaa
     2101 tcgcaagcgc gcgctgtccg gcctcgtccg cctcttgatg ctgtccgagc gcttcatata
     2161 gggaagccag cagcgcccaa gacgcggcct cccctgacgc atcgcgtaac gattgataga
     2221 gggtcaccgc gcgcttcgcc gatcctattc ctttctcctg ttgaccgatg gcgccataga
     2281 cagcagacat gcgataacgc aacgccgcct ccataccggc ttgaggtctt tgctcgacca
     2341 gtgctagcgc ccgttcgtaa tactgcttcg ccgagtcagg ctcatccatg atccaacggg
     2401 catcgccgag ggcaagcaat gccgcgagtg tctgatcgtg gtcctgacgg ttgtgggctt
     2461 ccttgagtgc ctggtcaagg agctggagtc cttcttgggt caacccgtcg ctgatccgta
     2521 ggagcccgag ttgtattctt gccgaaacca ccccttgaac attgcgcgac cggttgtatc
     2581 ggtccaccgc tcgttcaaac aagcggccgg cttcttctcg cgatcccaac caggcagaaa
     2641 ctttgccgag cgtcaacagc gcatctccat gagccgggct ggcgggaact tttgagaaga
     2701 gcgccaaggc atcacgaagc t
//
LOCUS       DATNDD010000095         4526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3720767, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000095 DATNDD010000000
VERSION     DATNDD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720767"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN647_02160"
     CDS             complement(<1..249)
                     /locus_tag="VN647_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWG95862.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAESKGNHVGDGQGV
                     GDNIYVIAHDRCTECVGHFDEPQCAAVCPVDNCCISDPL"
     gene            475..975
                     /locus_tag="VN647_02165"
     CDS             475..975
                     /locus_tag="VN647_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWG95863.1"
                     /translation="MTSDQAQTSNREQRKKRIIMMSLLALLLMSASVFLVERKESSII
                     VVTFPSGAELEAEVADTPEKLLFGLAFRDALPPNGGMLYIFEQNGMHRVTTKEYRFPI
                     DILWVDESHHVVHMLEHAEPCAHEPCPLLGPPLEAVRYVIQAESGFIKKAGVAKSDEL
                     KYALRM"
     gene            1007..1324
                     /locus_tag="VN647_02170"
     CDS             1007..1324
                     /locus_tag="VN647_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWG95864.1"
                     /translation="MDEFQKVAQLDEVPPGKSKIVKINDRSIALFNVEGTLYAIHNSC
                     PHEGAPLIEGRLKGYVVACPWHDLAFDIRNGQGIDGGGYCVGSYEVRVDGNDILIGPR
                     RKL"
     gene            1378..1716
                     /locus_tag="VN647_02175"
     CDS             1378..1716
                     /locus_tag="VN647_02175"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HWG95865.1"
                     /translation="MSISSADHNPALDDSHCRKVMVDQSFFIHLWEDRTRGEQWVPSY
                     DTQGLALLSDEFLCVASNNAVENGQRIFEFKAVQSGVHQLIFEKRMGWKFTAEDRRLF
                     RIEAEPPSRN"
     gene            1721..2560
                     /gene="dat"
                     /locus_tag="VN647_02180"
     CDS             1721..2560
                     /gene="dat"
                     /locus_tag="VN647_02180"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HWG95866.1"
                     /translation="MPDIAFINGRFLSWEEATVSIDDRGFQFGDAVYEVIRAYRGSPF
                     ELAAHLARLDRSARELSLRQPYTSVQWTRWIQQGLSLAGYQDAKVYIQITRGVAPREH
                     SFPSDILPTVVMTIRRFHPLAPEVRLAGVSACTREDLRWGRCDIKSVNLLANVLAREE
                     AKKVGVFETILVRDGFVMEGALSNVMAVQDGVVITAPESTRILSGVTRTVVLELAKKD
                     DIVIEERFIPVDLLYRADEVFLTGTTLEVLGVVQIDGKTIGSGQPGPITKMLAARWAL
                     LTG"
     gene            2658..3146
                     /gene="rimP"
                     /locus_tag="VN647_02185"
     CDS             2658..3146
                     /gene="rimP"
                     /locus_tag="VN647_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWG95867.1"
                     /translation="MSIPGNGPQPVADRLREIISPILWTLGLELVDVVCVGQGPRSII
                     RVLIDKSGGVSIADCEQAHKALGPALDVADPFPHAYTLEVSSPGLDRPFKRSQDYQRA
                     IGKQVSLKLRQPLEGQWRITGQLMQVDEQAVVLIVAAMRTPQTVKLDRDMIAEAKLVV
                     KI"
     gene            3199..4350
                     /gene="nusA"
                     /locus_tag="VN647_02190"
     CDS             3199..4350
                     /gene="nusA"
                     /locus_tag="VN647_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWG95868.1"
                     /translation="MNRELISVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKIIVETVGNPKAEISLQEARQYDSEAEIGDEIGSLIEMNEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVTGIILGMERRNYLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKAMLLEVRRTPKDVQVILSRSHPQFVAKLFELEVPEVMEK
                     IIEIRSVVREPGDRTKIAVTSREKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     QDPRVFIAEALNPATIEKVGIDEEKKSALVVAADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKVERDKEIKAALAEEAEAQRLQEEARQAVRAEEATSG"
     gene            4372..>4526
                     /locus_tag="VN647_02195"
     CDS             4372..>4526
                     /locus_tag="VN647_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HWG95869.1"
                     /translation="MRVYELAKKLGMENRVLIPELKKMGASVSSHSSTLDDEIVQRAL
                     DKLGSKS"
BASE COUNT         1116 a         1097 c         1330 g          983 t
ORIGIN      
        1 gagagggtct gaaatacaac aattatcaac ggggcaaacc gcagcacact gaggttcatc
       61 aaagtgcccc acgcattcgg tgcagcgatc atgcgcgatt acgtaaatat tatcaccgac
      121 gccttgcccg tcaccaacgt gattgccttt gctctcagcg tcgcttctgg tttcaaagat
      181 tgcttcatta ggacattccg gcaggcatgc tccacaagag atgcattcat ccgtaatcaa
      241 taaagccatg acccgtgcct ccttctcacg ccaaaagact atggaacatt gacaagttca
      301 cgcggctcac accatagtgc ttccgtcgac ttatggcgag taagcacgtg aacacgcatt
      361 ttattccgcg attcttttct aagtcaactg aacggagtcc ggtcagaagc cctaggtcat
      421 tattgtgcct gggccattgg gcccagcgga gaaagaaaac gtcggtcgtc atcaatgaca
      481 tctgatcaag cccaaacgag taatcgagag cagcggaaga agcggatcat tatgatgagt
      541 ctgctcgctc ttctcctgat gagcgcgtcg gttttcctcg tcgaacgcaa ggaatcgagc
      601 attatcgtcg tgacattccc gagcggtgct gagctggaag ccgaagtggc ggacacgccg
      661 gagaagctgc tctttgggct tgcctttcgc gatgcgttgc ctccaaacgg cggcatgctt
      721 tatatctttg agcaaaacgg gatgcatcgc gtgacgacca aggaatatcg attccccatc
      781 gacattctgt gggtggatga gagtcatcat gtggtgcata tgctggaaca tgcagagcca
      841 tgtgctcacg agccttgccc ccttttgggg ccaccactcg aagctgttcg gtatgtgatc
      901 caggcagaat cggggttcat taaaaaagcg ggagttgcaa aaagcgacga gctcaagtat
      961 gcccttcgca tgtagaggtt gggacacagc aagaggaata cgcacgatgg atgagtttca
     1021 aaaggttgca cagctcgatg aggtaccacc gggaaagtca aagatcgtca agatcaatga
     1081 ccgctcgatc gcgttgttca acgtcgaggg aacgttgtat gccatccata atagctgtcc
     1141 tcacgagggt gctcctctta ttgaaggaag actcaaaggg tatgtcgtgg cttgtccctg
     1201 gcatgacctg gcgttcgata tcagaaatgg gcaaggtatc gacggcggag ggtactgtgt
     1261 aggaagttac gaagtccggg tggacgggaa cgatattctg atcggccctc ggcggaaact
     1321 ataaaaaggg agatccggct gaacggttgt cttcacgacg cggatcaggg tatcgacgtg
     1381 agtatttcca gtgccgatca caaccctgct ttggacgaca gccattgtcg caaggtcatg
     1441 gtcgaccagt cattcttcat ccatttatgg gaagatcgca ccagaggtga gcagtgggtg
     1501 ccgtcttacg acacccaagg acttgcgctt ctcagtgatg agttcctctg cgtcgcgagc
     1561 aataacgccg tggaaaacgg gcagcgcatc ttcgagttta aagccgtgca gtcaggtgtt
     1621 catcagctga ttttcgaaaa acgcatggga tggaaattta ccgctgaaga tcggcgcttg
     1681 ttcagaattg aagcagagcc accatccagg aattgatctc atgcccgaca ttgcgttcat
     1741 caacggccgc tttctttctt gggaggaggc gactgtttcc atcgatgatc gagggtttca
     1801 atttggagat gccgtctacg aagtcattcg agcctatcga gggagcccgt ttgagcttgc
     1861 cgcgcatctt gctcggttgg atcggagtgc aagagagctc tcgcttcgcc aaccctatac
     1921 gagcgttcag tggacgcggt ggattcaaca aggactcagt ctggccggat atcaggatgc
     1981 caaggtctat atccagatta ccagaggggt ggctccccgt gagcatagtt tcccctccga
     2041 tattctcccg acggttgtca tgaccatcag aaggtttcat cccttggcgc cagaagtccg
     2101 cctggccggc gtcagcgcat gtacaaggga ggatcttcga tggggacgct gtgatatcaa
     2161 aagtgtcaat ctcctggcca acgtccttgc ccgtgaagaa gcgaagaagg tcggtgtctt
     2221 cgaaaccatt ttggtcagag acggcttcgt catggaaggt gcgctgagca atgtcatggc
     2281 tgttcaagat ggggtcgtca tcacggctcc ggaaagtacg cgaatcctat ccggcgtcac
     2341 acggaccgtg gtgttagagc tggcgaagaa ggatgacatc gtgatcgagg aacgattcat
     2401 accggtcgac cttctgtacc gcgcggacga ggtatttttg accggaacca cgcttgaagt
     2461 actcggggtg gtccagatcg atgggaagac catcggctcc ggccaaccag gtcctatcac
     2521 aaaaatgctg gccgctcgct gggcgttgtt gaccggctag tacccttgct ttgcactggg
     2581 gtgcatggta tggtgctccc tctgtaagtg ggctcatgcc cacttttttg tttggacaca
     2641 tgtcaaaggg aaatgggttg agcataccgg gcaatggtcc gcagccggtt gccgaccggt
     2701 tacgcgagat catttcgccg atcctatgga cgctcgggct tgagttggtt gatgtggtgt
     2761 gcgtggggca aggtcctcgc tcgatcattc gtgttctgat cgataaatct ggtggagtca
     2821 gcatcgcaga ctgtgagcag gcacataagg ctctagggcc ggcgctcgac gtggccgatc
     2881 cgttccccca tgcctatact cttgaagtct cttctccggg tctggatcga cccttcaaga
     2941 gatctcagga ctatcaacgg gcaattggaa aacaggtgag cctcaaactt cggcaaccgc
     3001 tcgaaggcca gtggaggatc accgggcagc tgatgcaggt ggatgagcag gcggtagttc
     3061 tgatcgtggc cgcgatgcgg acacctcaga cggtgaaact ggatcgagac atgattgctg
     3121 aggcgaagct ggtcgtgaaa atttaggggc tggagagttg gagattgtaa gcggtaaccg
     3181 gatgcaggag cgtgacgtat gaaccgagaa ctgatctcag tgatcgacga aatcgggcgt
     3241 cagaaaggaa ttgacaaggc ccgagtcatc ggggccattg aatccgccct gcagacggcc
     3301 gcgaagaaac gcttcggcca ggccgaaaac atccaagtgg agatcgaccc gaaaaccggg
     3361 gaaatttccg tcgtttccaa gaaaatcatc gtcgaaacag tcggcaatcc caaagcggag
     3421 atctctcttc aagaagctcg ccagtatgat agtgaagcgg agatcggcga cgaaatcgga
     3481 tcgctgatcg aaatgaacga attggggagg attgccgcac aaacggcaaa acaagtcatc
     3541 ttccagaagg tgcgtgaggc ggaatgggaa gcggttcaaa aagaatattc gacgcgtcag
     3601 ggggatctcg ttacgggaat cattctcggc atggaacgcc ggaattacct ggtggacctc
     3661 ggaaagacag aggccatcct gccgatccaa gagcagattc cgcgtgaaac atatcggcgc
     3721 ggcgatcgtg tgaaagcgat gttgctggaa gttcgtcgta cgccgaaaga tgtgcaagtc
     3781 attctgtccc gtagccatcc gcagttcgtg gcgaagcttt ttgaacttga agtcccggaa
     3841 gtcatggaaa agatcatcga aatcaggtcc gtcgttcgag agccgggaga tcggacgaag
     3901 atcgcagtca cgtcacgtga aaaggccgtg gatccggtgg gggcttgtgt cggtattaaa
     3961 ggctcccgcg tgcaggccgt cgttcgcgag ttgcggggcg agaagattga tatcattacc
     4021 tggacccagg acccgcgggt ctttattgca gaagccttga accctgcgac gatcgaaaag
     4081 gtaggaatcg acgaagagaa gaagtcggcg ctcgtggttg cagccgactc gcaactgtcg
     4141 ttggcgatcg gcaaaaacgg tcagaatgtc cggcttgccg cgcgtttgac gggatggaag
     4201 atcgatatca tcagcgcaac ggaatacgaa aaggaaaaag tagaacgcga taaggaaatt
     4261 aaagcggcgc tcgcagagga agccgaggct caacgactgc aggaagaagc tcggcaggct
     4321 gtcagagccg aggaagcaac gagcggatag aaacggaact gtctcatggc tatgcgcgta
     4381 tacgaactcg cgaagaagtt agggatggaa aatcgagtgc tcatccccga gctcaagaag
     4441 atgggggcat cggtttcctc tcatagcagt acgctcgacg atgaaatcgt tcagagagca
     4501 ttggataagc tgggctcaaa atcgaa
//
LOCUS       DATNDD010000096         2422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1555780, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000096 DATNDD010000000
VERSION     DATNDD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555780"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VN647_02200"
     CDS             complement(<1..534)
                     /locus_tag="VN647_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWG95870.1"
                     /translation="MQLDEPEDEELSPTDPNEFPAGIELSPAPEHETGPRSDTTAVVP
                     VTALQQYLAEVRRYPYLSKEEELQLFQEYQTHGSREAAVKLIMANLRVSVSIAAEYLH
                     TGADHMDLIQEGNVGLMQAIKKFDPSKNVRFYAYAAWWSRAYILRYLLHTFRLVKVGT
                     TQDQRKLFYNLKKEKAKL"
     gene            841..2049
                     /locus_tag="VN647_02205"
     CDS             841..2049
                     /locus_tag="VN647_02205"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWG95871.1"
                     /translation="MATPFDSIENAIRDIKKGKCIILVDDEDRENEGDLVIAAEKVTP
                     HVINFMATHGRGLICLTLTPQRVEELQLPQQASENTATFGTAFTVSIDARKDITTGIS
                     AADRATTIHVAIDPKSKPSDLARPGHVFPLKAQTGGVLKRAGQTEGSVDLARLAGLCP
                     AGVICEIMNVDGTMARVPELTKFAKSHKLRMVTVKALIEYRMRRETFVRRMASARLPT
                     TFGEFEAVAFENQIDGVTHIALVKGPVDGGAPTLVRVHSGCLTADALGSLRCDCRDQL
                     HSAMEIIQKEGRGVLLYLNQEGRGIGLLNKIKAYGLQDQGSDTVEANLQLGFKADLRD
                     YGVGAQILANLGLHQIKLITNNPRKIVGIEGYGLKVVERVPIEIPPRAANVRYLRTKK
                     AKLGHLLKKV"
     gene            2108..>2422
                     /gene="ribH"
                     /locus_tag="VN647_02210"
     CDS             2108..>2422
                     /gene="ribH"
                     /locus_tag="VN647_02210"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWG95872.1"
                     /translation="MKYRKTEASAAGFRIGIVAAKFNKEVTSKLLNACVDTLTAYGVR
                     KENMHVMRVPGAFEIPLVARAMAQSGRFNAVICLGAVIRGDTPHFEYISAEVSRGIGQ
                     AAL"
BASE COUNT          566 a          591 c          718 g          547 t
ORIGIN      
        1 aagcttggct ttctcctttt ttaagttgta gaacagtttt cgctgatcct gagtcgtccc
       61 gaccttgacg agccgaaagg tgtgtaagag gtaacggagg atataggctc tggaccacca
      121 ggcggcatag gcatagaagc gcacgttttt cgagggatcg aatttcttga tggcttgcat
      181 caggccgacg ttcccttctt gaatcagatc catgtggtcg gcgcctgtat ggaggtactc
      241 ggctgcaata gaaaccgaca cacgcaggtt ggccatgatc agtttaaccg ccgcctcgcg
      301 gctgccgtgt gtttggtact cctgaaaaag ctgaagctcc tcttctttcg acagataggg
      361 gtatcggcgg acttcggcaa ggtactgctg tagggctgtg accggcacca cggcagtcgt
      421 gtcggatcga gggcctgtct catgctcggg agcaggactc agctcgatgc cggcaggaaa
      481 ctcgttcggg tccgtcggag agagctcctc atcttcaggc tcgtccagct gcatatcggt
      541 ctcgtcgagg atggacatac agagggcaga ctacaccaga ctcctaggtc gcaacaatgc
      601 tgaaagcggg cccgagtgcc ttatttgaac agggagaggg taaaagcaga cacgcctcct
      661 gatatgatgc gaggatgttc agcagtgaag cctccttttt ccgacagaac tcaggtgaat
      721 ttctgtcgat ttttttggtt gacagccggg agagaaacaa ggttatgtgt caccctccta
      781 gttgctaaat cttcgatgtt taatggtaaa ttgtcgtatc gtgagcgata ggacttagac
      841 atggccactc cgttcgatag catcgaaaat gcgattcgcg acatcaagaa aggaaagtgc
      901 atcatcttgg tggatgatga ggatcgggaa aatgagggtg acctggtcat tgccgcggag
      961 aaagtcactc cgcacgtgat caacttcatg gccacgcatg gccgaggcct gatttgtctg
     1021 actctgacgc ctcaacgggt ggaagaacta cagttgccac agcaggcttc cgagaatacc
     1081 gcgacgttcg ggaccgcctt taccgtctcc atcgatgcac gaaaagacat tacgacgggg
     1141 atctcggccg ccgatcgggc cacaacgatt catgtggcga tcgacccaaa atctaagccc
     1201 agtgatctcg cgcggcctgg tcacgtcttt ccgctcaagg cacagacagg tggagtactt
     1261 aaacgggccg gccaaaccga aggatctgtc gacctggcta ggttggcagg gctatgccct
     1321 gccggagtga tctgcgagat catgaatgta gacggcacta tggcccgcgt acccgagctg
     1381 acgaaattcg ccaagtccca taagctgagg atggtgaccg tgaaggcctt gatcgaatac
     1441 cgcatgcgga gggaaacctt tgtcagacgc atggcaagcg cacgattgcc cacgacgttt
     1501 ggagagtttg aagccgtggc gttcgagaat cagatcgacg gggtgacgca cattgccttg
     1561 gtgaagggcc ctgtcgacgg cggagcgccg acactcgttc gtgtacactc cggatgtttg
     1621 acggccgacg cattaggatc gttgcgttgc gattgtcgcg atcaattgca ctctgcgatg
     1681 gagatcattc agaaggaggg tcgaggggtt cttctgtatc tgaatcaaga aggccgagga
     1741 atcgggttac tgaataagat caaggcctat gggttgcagg atcaagggag tgatacggtt
     1801 gaggccaatc tgcaactcgg attcaaggcg gatctccgcg actatggagt cggcgcgcaa
     1861 attttggcga atctcggcct gcaccagatc aaattgatca cgaataatcc gcggaaaatc
     1921 gtcgggatcg aaggctatgg tcttaaggtc gtcgaacgtg ttcccattga gatccctccg
     1981 cgcgccgcga atgtccggta tcttcggaca aagaaggcga agctggggca ccttctcaag
     2041 aaagtttgat gagctgcttc agctcaagtc cgatctctgg ggtttcgtac agggcggcaa
     2101 tggaaaaatg aagtacagaa aaacggaagc ctcagcggct ggatttcgga tcggcattgt
     2161 cgcggcgaaa ttcaataaag aggtcacgag caaattgctc aatgcttgcg tcgatacgct
     2221 taccgcctat ggtgtccgta aagaaaacat gcatgtcatg cgggtaccgg gggcgttcga
     2281 aataccgctt gttgcgcgcg ccatggccca gtcaggtcgg ttcaatgcgg tgatttgctt
     2341 gggggcggtc attcgaggtg acaccccgca ttttgagtat atcagtgccg aagtcagtcg
     2401 aggcatcggt caagcggctt ta
//
LOCUS       DATNDD010000097        10181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4115079, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000097 DATNDD010000000
VERSION     DATNDD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4115079"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VN647_02215"
     CDS             complement(<1..794)
                     /locus_tag="VN647_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95873.1"
                     /translation="MSATELIELWTTRLEAERVRLTETGQDAPVVASQGRLVQTTGGL
                     HLYEFIVPDDVQLSVDLPVSVIPADEADTTEGIVLRQIGNRVYVQLVDPLGRDVPSVT
                     LVPDQAGLVGTSASRLKDMLTKPDLYHLGPAERLASLLQMQEAEAAAFSSASSVFTTM
                     WSDDRSFRRQKLANLAMELIRANNRILLISPDHQECDEIAGMVGRTMKGGGLNHKTWI
                     TRYELPIASQAGGVDLHELGFEAQMHQFYAKSQGDKASLKHKYDRFR"
     gene            1075..1344
                     /locus_tag="VN647_02220"
     CDS             1075..1344
                     /locus_tag="VN647_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95874.1"
                     /translation="MKGLRFERIAKGRHYNVVFHIGSTYVPVSDDTVEELKGQSLLPA
                     ERFFDFLIDRVGYSSYLKDQIRNELKATGDPTTQITVLQGAIREL"
     gene            complement(1526..1927)
                     /locus_tag="VN647_02225"
     CDS             complement(1526..1927)
                     /locus_tag="VN647_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWG95875.1"
                     /translation="MIVADTNLLIYLYVQGERTEESEAVLRRDATWTVPLLWRSEFRN
                     ALIGLVRKEVLAVDQALSIVDETERWLAGYEYSVISRHVLTLADRSGCSAYDCEFVAL
                     AQDLGIPLVTTDRQILKAFPRLAISPDGFAR"
     gene            complement(1924..2166)
                     /locus_tag="VN647_02230"
     CDS             complement(1924..2166)
                     /locus_tag="VN647_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95876.1"
                     /translation="MASLTIKNIPEPLVKRLKQQAAAQHRSLNFQVISYLEQMTQSVP
                     VDADTLLARARAIRQIPKGLKLTDRLLKELKVVGRP"
     gene            complement(2259..3290)
                     /locus_tag="VN647_02235"
     CDS             complement(2259..3290)
                     /locus_tag="VN647_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95877.1"
                     /translation="MNAHQQQCEAAIVAGAWDTLFAESMAWSQDPDGTKDPRPLFARN
                     VVYLLQGRFADAWKAHALCLEAEKDIAAVGTWVKDLLDRHTDSGYTNLVMGLFLAQSG
                     QSEQSMRPYSEAARLLPQSAYPHYFLAQVHERAGRLEMAIKGYREAVRFAPDYAPARM
                     NLGVAYQDQGRLEMAIKEYREVVKLTPTDSAAHSNLACALAEQGKMEPALQSYKTALK
                     LNPQDAEVHFALGGLYEMRGRLDLAQKSYQDALNADPDFGAAHSALGWLALGKHRLQE
                     AADIFSRALKCNEEDARAYHGIAEIYALRGKRQSAMENYTKALKYYRDPEKKNQIMNQ
                     LFQQGQVGD"
     gene            complement(3306..4793)
                     /locus_tag="VN647_02240"
     CDS             complement(3306..4793)
                     /locus_tag="VN647_02240"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWG95878.1"
                     /translation="MSQDQSGRRVMAIAPMPPEKSAYALARYSRSPDSIEQSIRWVHG
                     HSSEKFWDQFYFDYGHASIADLGHVIICFEEISELAAIRLEDEPLWDGQAKSSRYQNF
                     ASDRWFVPGQIRGSETEAVYEGILRSLSEIYRLLHDPLIAHLSERDPRPESMKASDYQ
                     RTIAARAFDVTRYLLPLAAKTNVGQVVSIRTLEKQITRLLSSQLPELRAIGEDLKEAC
                     QRPPVNLWNELSGQTLNLNDPLAPTLARHAKTNAYQESVYSDVARHAKEALRGTGLDQ
                     PGTWGAVESVELIDPHHPLDEIVTTLLYRVSQAPYRKILEIVKEWSEKEKQEIIEVAT
                     RQRGPYDELIKEFRSGYAFNFDILMDIGAWRDMHRHRRCQQVQQNFTTLHGYDVPSPL
                     IEAGLDQEYRQAMDAVRQDIELLKKKDQEASLYAIPFGFKVRCLFKMDYAEAEYISKL
                     RSGVKGHWSYRTVAWKMKQQLARRYPCLGEQIHATPPDVEDALTR"
     gene            4958..6220
                     /gene="hisS"
                     /locus_tag="VN647_02245"
     CDS             4958..6220
                     /gene="hisS"
                     /locus_tag="VN647_02245"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWG95879.1"
                     /translation="MIKGIKGVKDLLPEETPCWRLIEETARRWAGRYGFHEIRIPIFE
                     VTTLFARSIGTSTDIVEKEMYTFQDRDGTSLTLRPEGTAGTVRAYIEHNRAAIPVPQK
                     YFYFGPMFRHERPQAGRLRQFHQFGVESLGMADPQADIEVIALLWRILSDLDLPGLTL
                     EINNLGYTADRDLYRPHLVSYLKQHESGLCANCRHRIDANPLRVLDCKAPDCRAITET
                     APRLSDSLSEAARSYFIRVLSGLDVVKIPYSLNHRLVRGLDYYNLTTFEVTATNLGAQ
                     NAVGAGGRYDGLVETLGGPPTPAVGFAIGIERMSILLPESTVRKNPEDVTVYVAGFGG
                     QGTVAGFTALEELRLAGFQAVSDFRSLTLKAHLRQADRLRCRFTLIIGDDEVVKGSAI
                     LRDMETKAQHDVSLSTMALQIPSLLAGS"
     gene            6314..6634
                     /locus_tag="VN647_02250"
     CDS             6314..6634
                     /locus_tag="VN647_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWG95880.1"
                     /translation="MDSKKIGFLLALPPSHTSAETVHGLAQAALDAGHQVYLYLIDEG
                     VKNIHTDHYRGLAQAGARVFACAYGCQQHHVPTATIDPKMSLCGLVVLSGIIDACDPF
                     LSFT"
     gene            6645..6986
                     /locus_tag="VN647_02255"
     CDS             6645..6986
                     /locus_tag="VN647_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95881.1"
                     /translation="MAKNIAIVIQEDPRKSHRPVEALRIAVGLASGSHVTTVVLLGEA
                     VRLLGDETDDILDVEILEKYLPAIEQLEILFILQDTPEQIPVRDEFSIRRETDETIGS
                     FVRSMDCALVF"
     gene            7003..7224
                     /locus_tag="VN647_02260"
     CDS             7003..7224
                     /locus_tag="VN647_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95882.1"
                     /translation="MLAVVYVVRSPLHTLSQALFSVDDSAVVLSLENPLLPGKVLRAT
                     NGTGLTEGEQLSYERVLAIVLESNKVMTL"
     gene            7556..8899
                     /gene="dnaA"
                     /locus_tag="VN647_02265"
     CDS             7556..8899
                     /gene="dnaA"
                     /locus_tag="VN647_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWG95883.1"
                     /translation="MSIDTVWQDALQYIQRKVPKQVYDTWFIPVHLDRIENSTVHIGV
                     PNKFFGEWLDTHYGSLLAEAMAMARGGELMAVAFTVREKRVTLPGEPQAVVNGPRAVM
                     QSKPRRGIQLNPKYTFKNFVVGAGNQFAHAACMAVAEQPGHTYNPLFIYGGVGLGKTH
                     LLNAIGNHVAEKSDLRIAYLTTEQFTNEVINSIRYDKMMDLRKRYRHIDMLMIDDIQF
                     LVGKERTQEEFFHTFNALYEGHKQIVLSSDRFPKDMPDIEERLRSRFEWGLIADLQPP
                     DVETRIAILRKKSEDEGVKLPEDVIQFLSITMKSNIRELEGSLVRLGAYASLTGQVIT
                     LDLAKNVLRDLIGDKKKIVAMDDIQEAVCAQFHVKMTELKSRRRSKTLVHPRQIAMYL
                     CRELTDASYPEIGRQFGGKDHTTIIHACRQVAKAKETDTMLQTTIETLKEQILRN"
     gene            8923..10050
                     /gene="dnaN"
                     /locus_tag="VN647_02270"
     CDS             8923..10050
                     /gene="dnaN"
                     /locus_tag="VN647_02270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWG95884.1"
                     /translation="MKVRIGRDELLTGLQRVQGVVEKRNTMPILSNILLEAKQDGVEI
                     VATDLEIGMRGLYKGTVLSPGGVTISARKLFEIVKELPPGEIELTATDNSWTTIQAGK
                     SQFKVVGLPSSEYPALPTIEREGLTPLSGEGLLELIRKTLFAAGDNDARYILNGLLVT
                     LAATDKKTSLRLVGTDGHRLAVAEQEVGKAGSKGLPQEIKAIIPKKAAHEIRHLLEEG
                     GDAEPLIGFSKNLMIFRKSGLLLTSRLMEGNYPNYQQVIPKENGKKISVSRSELESAL
                     RRVSVLAKDKASAVKVSFASGMMTLFSSSPDYGEATEELPARYEGETINKGFNARYLL
                     DVFNVMDGETISLQMETPLSPCLIQEPESLGFKCVVMPIKV"
BASE COUNT         2337 a         2681 c         2724 g         2439 t
ORIGIN      
        1 cggaagcggt cgtacttatg tttcaaggag gccttgtcgc cttgtgactt ggcatagaat
       61 tgatgcattt gcgcttcgaa ccccagttcg tgaagatcga cgccgccggc ttgtgaagca
      121 atcgggagtt catatcgagt aatccacgtt ttatggttca gtccgccgcc cttcatggta
      181 cgccccacca tccccgcaat ctcatcgcac tcttggtgat cgggactgat caggaggata
      241 cgattgttgg cgcgaatgag ttccatcgcc agatttgcga gtttctgacg acggaatgac
      301 cggtcatccg accacattgt tgtaaagacg gatgaggcgg aggagaaggc tgcagcctca
      361 gcttcctgca tctgcaacag cgaggccaac cgctcggccg gcccgaggtg atacagatcc
      421 ggcttcgtca gcatatcttt cagacgggag gctgacgtgc cgacaagacc ggcctgatct
      481 ggaacaaggg taacggacgg cacgtcgcgt ccgagaggat cgactagttg gacgtatacc
      541 ctgtttccga tctgccggag gacaattcct tccgtcgtgt ccgcctcatc ggcagggatg
      601 acggataccg gaaggtcgac ggacagttgg acgtcgtcgg gcacgataaa ttcgtacaga
      661 tgaagtccac cggttgtttg caccaggcga ccttgcgacg ctacgacagg agcatcctgc
      721 cctgtttcag tcagtcggac tcgctcggct tcaaggcggg tggtccacaa ttcgatgagt
      781 tctgttgcgc tcatgaggac tgatctcatc tgcatctcaa ggcaaggacc ggtcatgata
      841 aatgcctgcc gcgatacctg tccaccgcag ggatcgagag gttgcccttg tcttcatgac
      901 cggatcttgt ctagaatgat ccccttctat tagcacttgc aggatgttca aaaagaccgt
      961 ccagcgaggc cgcaacgagc gagatgctga ggcatacttc cgagtatgtt gaggcattga
     1021 gcgatgcgag aacgaagctg gcggactttt tcaacatcct gcggaggagg atctgtgaag
     1081 ggcttgaggt ttgagcggat tgccaaagga cgacactaca acgtggtctt ccacatcggc
     1141 agcacgtatg tgccggtgag tgacgacacg gtagaagagc tcaagggaca aagcctgtta
     1201 ccggccgaac gatttttcga tttcctcatt gatcgcgtgg gctactcgtc ctacctgaag
     1261 gatcagatcc gcaacgaact gaaagcgacc ggtgatccca ccacacagat tacggtcctg
     1321 cagggggcaa tccgcgaact gtaacgcctg ctaaattttg ctcgctctct tctcgtcctc
     1381 tttctgcctc gacctatgcc tcacattctg aaatcttgaa tttcgatctt tggccatgtt
     1441 gccaaggttt ttgcctgtaa ctctggcaac ttggtgtagt tttccatcgc tctccggcgc
     1501 ttgcgccgga gggcagggct tccccttaac gtgcgaaccc atctggcgat atagcgagac
     1561 gggggaatgc tttcaggatc tgcctgtcgg tagtaaccaa cggtattccc agatcttgcg
     1621 cgagagctac aaactcacag tcatatgccg aacacccaga tcgatcagca agcgtcaaaa
     1681 catggcgaga gatcacgctg tattcatatc cggctaacca tcgttctgtt tcatcgacaa
     1741 tggaaagtgc ctggtctaca gccaatacct ctttccggac taggccgatc agcgcattcc
     1801 gaaattctga acgccacagt aacggaacgg tccacgtcgc atcgcgcctt agtacggcct
     1861 cgctctcctc tgttctttca ccctggacat agagatagat caacaaattc gtatcagcca
     1921 cgatcatggc cgaccgacca ccttcagctc tttgagaagg cgatcggtca atttcagccc
     1981 tttgggaatt tggcgaatgg ctcgcgctct tgccagcaat gtgtcggcat caaccggcac
     2041 gctctgtgtc atctgttcca gatacgagat aacctgaaaa ttcaggctcc ggtgctgagc
     2101 tgccgcctgc tgtttgagtc gtttcaccaa tggttcggga atgttcttga tggtcaagct
     2161 cgccatagat tatcctcatg gttccatatt gcatccgtta tggatgctat accgaacgtc
     2221 aagatcccgt caagcgagaa gagttgacgg gcgatccctc aatccccgac ctgtccttgc
     2281 tggaacagct ggttcatgat ctgattcttt ttctcgggat cgcgatagta cttcagggct
     2341 ttggtgtagt tttccatcgc actctggcgc ttgccccgca gggcatagat ttcagcgatg
     2401 ccgtgatagg cccgtgcgtc ttcctcattg cacttgagcg cccggctgaa gatatccgcg
     2461 gcttcttgaa gccgatgttt gcctaaggca agccagccga gcgcagaatg ggcggcgcca
     2521 aagtccggat cggcgttgag tgcgtcttga taactctttt gtgccaggtc caggcgtccg
     2581 cgcatttcgt agagtccgcc cagcgcgaag tggacttcgg cgtcctgtgg gttcagtttc
     2641 aaggccgtct tgtaggattg caacgcgggt tccatcttcc cctgttctgc gagtgcacag
     2701 gcgagattgg agtgggctgc cgaatcggtg ggggtgagct tcaccacctc ccggtattct
     2761 ttgatcgcca tttccagccg cccttggtct tgatatgcga cccccagatt catccgtgcc
     2821 ggcgcgtagt ccggtgcaaa ccggacggct tcacgatacc ccttaatcgc catttcaaga
     2881 cggccggcgc gttcatgaac ctgtgccaga aagtaatgcg gataggcaga ttgcgggagt
     2941 aatcgagcgg cttctgagta tggtctcatg gattgctccg attgacccga ttgcgccagg
     3001 aaaaggccca taaccaaatt ggtgtagcca ctgtccgtgt gccgatcgag cagatccttc
     3061 acccacgttc ccacggccgc gatgtccttc tcagcttcga ggcagagcgc atgggctttc
     3121 caggcatccg caaaccgacc ctgcaagaga tatacgacgt ttcgcgcgaa gagaggacgt
     3181 ggatcttttg ttccgtccgg atcctggctc cacgccattg actcggcgaa caaagtgtcc
     3241 catgccccgg ccacgattgc ggcttcgcat tgttgctgat gggcgttcat ctgtgagaca
     3301 acgtcttatc tggtcagcgc gtcttcgaca tcgggtggcg ttgcgtggat ctgttcaccg
     3361 aggcagggat atcgtcgggc gagttgttgc ttcatcttcc acgcgacggt tcgatatgac
     3421 cagtggcctt tgacgccgga ccgtagcttg ctgatgtatt ccgcttccgc gtagtccatt
     3481 ttaaacaaac accgtacttt gaagccgaac ggaatcgcgt agagtgatgc ttcctgatcc
     3541 tttttcttga gcaactcgat atcctgacgg actgcatcca tcgcctgacg atactcctga
     3601 tccagacctg cctcaataag cggagacggg acatcatagc cgtgaagagt cgtgaaattt
     3661 tgctgcactt gctgacaccg tcgatggcgg tgcatgtctc gccaggcgcc gatgtccatg
     3721 aggatatcga agttgaatgc atagccgctg cggaactctt tgatgagttc atcgtatggc
     3781 ccgcgctgtc ttgtggccac ttcgatgatc tcttgcttct ccttctcaga ccactctttt
     3841 acgatctcga gtatcttccg gtacggggcc tgcgacactc ggtaaaggag tgtcgtgacg
     3901 atttcgtcga gcggatgatg tgggtcgatc agctcgaccg actccaccgc accccatgta
     3961 ccaggctgat ctaaccctgt gccgcgcaat gcttccttcg catggcgagc cacatcggag
     4021 tagaccgatt cctgataggc gttcgtcttg gcatgccgtg ccagtgtcgg cgccagagga
     4081 tcgtttaagt ttaaggtctg accgctgagt tcgttccaca gattcaccgg cggtcgctgg
     4141 caggcctctt tcaaatcctc gccgatcgcc cgcagttcag gcaattgtga cgacaacagg
     4201 cgcgtaatct gtttttccaa cgtccggata ctcacgactt gcccgacgtt ggtcttggcc
     4261 gcaagaggaa gcaggtagcg ggtgacatca aaggcgcgtg ccgcgatcgt ccgctgataa
     4321 tcggacgctt tcatagactc cggccgtggg tcccgttcag agaggtgcgc gatcaaggga
     4381 tcgtgcagta atcgatagat ttcggacaga ctgcggagaa tgccttcata gaccgcttcg
     4441 gtttcgctcc ctcgaatctg acccggcaca aaccatcggt cggatgcaaa gttctgatag
     4501 cgactggatt tcgcttgacc atcccaaaga ggctcatcct ccaagcgaat agcggcgagt
     4561 tccgagatct cttcaaaaca gatgatgacg tgaccgaggt cggcgatcga cgcatgaccg
     4621 taatcgaaat aaaactggtc ccagaacttc tccgaggaat gtccatgcac ccaccggatg
     4681 ctttgctcaa tggagtccgg cgatcggctg tagcgcgcta gtgcgtatgc ggatttctcc
     4741 ggcggcatgg gggcgatggc catgacccgg cgacctgatt gatcctgact catagaaggc
     4801 ctttcatgct gtactcaacc ataggccgcg cactatacct ctcacgtcag tatggcgcaa
     4861 gctgccgatc cgttgacttg ccgcagaagc cgccgctata gtccgcgcga actgtgccgc
     4921 atgatgcgca ccttcgtaca gagatgatcg ggtactcatg atcaaaggga ttaaaggtgt
     4981 caaagacctg ttgccggaag agacaccgtg ttggcgcctc atcgaagaga ctgccaggcg
     5041 gtgggccggt cgctacggat ttcatgaaat tcgcattccg atctttgaag taacgacctt
     5101 gtttgcgcgg agcatcggta catcgaccga cattgttgag aaggaaatgt acacgttcca
     5161 agatcgggac ggtacctcgt tgacccttcg acctgaaggg accgccggga cggttcgcgc
     5221 ttatatcgag cacaatcgcg ccgcaatccc cgttcctcag aagtatttct atttcgggcc
     5281 catgtttcgc catgagaggc cgcaagcagg ccgcctgcgg caatttcatc agttcggcgt
     5341 cgaatcgctt ggtatggctg atccccaagc cgatatagaa gtcatcgccc tcctatggag
     5401 aatcctgtcc gatctcgatc tacccggcct caccttggag attaacaacc taggctatac
     5461 tgctgatcga gacctctatc ggccccacct cgtaagttac ctcaagcagc acgaatccgg
     5521 cctttgtgcg aattgccgac atcgcatcga tgccaatcct ctgcgggtcc tggactgcaa
     5581 agcccccgat tgtcgcgcaa ttacggaaac cgctccccgc ttaagcgact cactgtccga
     5641 ggcagctcgt tcgtacttca tacgagtctt atcgggtctt gatgtcgtga agattccgta
     5701 ctcgttgaat catcggcttg ttcgaggtct tgactactat aacctcacca ccttcgaggt
     5761 tactgcgacc aacttgggtg cccagaatgc cgtaggggcc ggtggacgct acgacggatt
     5821 ggttgaaact ctcggagggc cgcccacacc ggctgttggt tttgcgatcg gcattgaaag
     5881 aatgtcgatc ctgctgccgg aatccacggt aagaaaaaat ccggaggatg tgactgtcta
     5941 tgtcgccggg ttcggtggtc agggtaccgt ggctggtttc actgctcttg aggaacttcg
     6001 tcttgcggga ttccaggcag tctccgattt tcggtcgcta accctgaagg ctcacttacg
     6061 gcaagctgat cgcctcaggt gtcgattcac tctcattatc ggggatgacg aagtagtaaa
     6121 aggatcggcc attcttcgcg atatggagac caaagcacag catgatgtga gtctctccac
     6181 tatggctctt cagattccct ccctccttgc aggctcctga aacgtacttt tttcaggttg
     6241 actttctcct caaaactcct taacctttca ctatatttat tatataacta tatagaatta
     6301 atcataaatt cctgtggatt caaagaagat aggctttctc ctggctcttc ccccctcaca
     6361 caccagtgcc gagaccgttc acggcctcgc acaagcggcc cttgatgccg gtcatcaggt
     6421 ctacttatat ctaatcgatg aaggcgtaaa gaatatccac accgaccatt atcggggtct
     6481 cgcacaggct ggggcccgcg tttttgcatg tgcctatgga tgtcaacaac atcatgttcc
     6541 aacggcaact attgatccga agatgtcact ctgcggcctc gtcgtgttgt ctggaattat
     6601 tgatgcttgt gatccatttt tgtcgtttac ctaatcatct tttcatggca aaaaatatag
     6661 ctattgttat tcaagaagat cctcgaaaga gccatagacc tgtcgaggct ctccgcattg
     6721 ccgtcggcct tgcatcggga tctcacgtga caaccgttgt tctccttggt gaggctgtcc
     6781 gtctcctggg agacgagacc gacgacattc tcgatgtcga aatccttgaa aaataccttc
     6841 ccgcgattga acagctcgag atcctcttta ttctccaaga cacacctgag cagataccag
     6901 ttcgggatga gttctccatc agacgagaaa ctgacgaaac tatcggctct tttgttcgat
     6961 ccatggattg tgcactcgtt ttttgattcg ggagttcctt ttatgctggc ggtcgtctac
     7021 gttgtccgtt cgccgcttca tacattgtcc caggcgctct tctctgtgga tgattctgca
     7081 gtggtgctct ccctggaaaa tccccttctc ccaggaaaag tattgcgtgc gacgaatgga
     7141 accggattaa cagaaggaga gcagttatcc tatgaacggg tgctggccat agtccttgag
     7201 agtaacaagg ttatgactct atgactccga agtatctatt taaagaaaat tcgtagtcgt
     7261 cggaataaga gtaggaaagt gaataaagag gataagcagg aggctattga ttttattaag
     7321 aaaatacaag ttcatcatga aggataacac tggggattgg ttctttatgg agtagtagga
     7381 acttgtgaag agtttgtgaa aggaaaaaaa gaactggagc ggcgtcttca cacagctcga
     7441 catgacccgg tgcgccatgg tattcaccgt cgcttttttg tcatgcaatt aaccacatcg
     7501 gaggattgtt atccacaggt gtgaataatc ctgtgtatag ggattttgag acgttatgag
     7561 cattgatacc gtttggcagg acgcactgca gtatatccaa aggaaggttc cgaagcaggt
     7621 gtatgacaca tggttcatac cggttcatct cgaccggatt gaaaactcga ccgtgcacat
     7681 tggagttccg aacaaattct tcggagaatg gttggatacc cattatggat cactgctggc
     7741 agaagccatg gccatggctc gtggcgggga gctgatggct gttgcattta ccgtccgtga
     7801 gaagagagtt accctacccg gggagcccca agccgttgtc aatggacctc gtgccgtaat
     7861 gcagtccaag cctcgccggg gaatccagct gaacccaaaa tatacattca agaattttgt
     7921 cgttggtgct gggaatcagt tcgctcatgc ggcctgcatg gcggtggccg agcagcccgg
     7981 gcatacgtat aacccgctct ttatttacgg tggagtggga ctgggaaaaa cccacctctt
     8041 aaatgccatc ggaaatcatg tggctgaaaa gagcgatctg cgcatcgcat atctgaccac
     8101 ggagcaattt accaatgagg tgatcaactc tattcgctac gacaaaatga tggatttgcg
     8161 gaagcgctat cgacacatcg atatgctcat gatcgacgat attcagttcc tagtcgggaa
     8221 agaacggact caggaagagt tcttccatac attcaatgcg ttgtatgaag ggcataaaca
     8281 aattgtcctc tcaagcgatc gttttcccaa agatatgccg gatatcgaag agcgcctacg
     8341 atctcgcttt gaatggggac tgattgcgga cttgcagccg cccgatgtgg aaacccggat
     8401 cgccatcctg agaaaaaagt cggaggatga gggagtcaaa ctgcccgaag acgtcatcca
     8461 gttcctgtcg atcacgatga agagcaatat ccgcgagtta gaaggtagcc tggttcgatt
     8521 aggtgcgtat gcctcgttga ccgggcaagt aataaccctt gatcttgcca agaatgttct
     8581 ccgtgatctc atcggggaca agaaaaagat cgttgcgatg gatgatattc aagaggcggt
     8641 ctgtgcacag ttccatgtga agatgacgga gctgaaatcc cgccgccgga gcaagacact
     8701 cgtgcatcct cgacagatcg ccatgtatct gtgtcgcgag ctgaccgacg catcgtaccc
     8761 tgaaatcggg cgccaattcg gggggaagga ccatacgacc atcattcatg cctgtcgcca
     8821 ggtcgcaaag gccaaggaga cggacacgat gttgcagacg acgattgaaa cattaaaaga
     8881 gcaaattctt agaaactaag agcttggccg ggagagatct caatgaaagt acgtattggg
     8941 cgagatgaat tgttgacagg actccagcgg gtacaaggtg tcgtggagaa acgaaacacg
     9001 atgccgattc tgtcgaacat tctgttggaa gccaagcagg atggggtgga gatcgtagcg
     9061 acggaccttg agatcggcat gcgagggctg tacaaaggga ccgttctttc gcccggcggt
     9121 gttaccatct cggcccgaaa actattcgaa atcgtcaaag agttacctcc cggcgagatc
     9181 gaactgacgg cgactgacaa tagctggacc accatccagg ccgggaagag tcaattcaag
     9241 gtcgtaggcc tccccagcag cgagtacccc gcactgccga cgatcgagcg tgagggattg
     9301 acaccgctct cgggagaagg acttctggaa ttgatccgga agacactctt tgccgccgga
     9361 gataacgatg cccgctatat cctgaacggc ctcctagtca ccctcgccgc gaccgacaaa
     9421 aagacctccc ttcgattagt cggcacagat ggccatcgtt tggcggtggc cgagcaggag
     9481 gtcggcaagg ccggcagtaa aggcctgccg caagaaataa aagccatcat acctaaaaag
     9541 gccgcgcacg aaatccgcca tctgttggag gagggcggag atgccgaacc gctcattggt
     9601 ttttcaaaga atctcatgat cttccgcaag agcggcttgc tgctgacgtc gcggttgatg
     9661 gaaggcaact atcctaacta ccagcaggtc attccaaagg aaaacggcaa gaagatcagc
     9721 gtgagtcgaa gtgaattgga gagcgcgctg cgtcgcgtgt cggtgttggc taaggacaaa
     9781 gccagtgcgg taaaggtttc gtttgcatca ggcatgatga ccttgttttc cagtagcccg
     9841 gattatgggg aagccacgga agaattgccc gcccggtatg agggtgagac cattaataag
     9901 ggattcaatg cccgttatct tctggatgtc ttcaacgtga tggacgggga aaccatatcg
     9961 cttcagatgg aaaccccgct gagtccctgt ttgattcagg agccggaaag cctgggattc
    10021 aagtgtgtgg tcatgcccat taaggtttag cgggatgctg aaaaagtcca tcgacatcgt
    10081 tctcgtatcg accataggaa acggatgacc acagacgact cttcccaacc caaatcagac
    10141 agctacagcg cggatcaaat caaggtcctc gaaggtctcg a
//
LOCUS       DATNDD010000098         6571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2075921, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000098 DATNDD010000000
VERSION     DATNDD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075921"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VN647_02275"
     CDS             complement(<1..1663)
                     /locus_tag="VN647_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWG95885.1"
                     /translation="MFNMAWRETRAAWRHFLYFLVCIAIGVGALTGVSLFGTQVERAV
                     TKEARGLLGGDLEIRLSRLMSPTGREVLDSLSDRGVALTHVSELVAMASRAGSSGSGQ
                     STQIIELKAVEPQYPLYGTLRLEAQGGLVGLLDRQARRCPDRTCFGVVVQESLLIRMG
                     LAVGDPLKIGQGLFIITGIVKTEPDRMANAFSLGPRVLMSREGLLTAELVKMGSRIRE
                     RYLLKIPSTMAPEPLLYELRGQLASDSARVSSYRDAQPQLKQSLEQLTRYLGLIGLTA
                     LFVGGLGVATSVHAFVREKLNTIAILKTVGAESSTIIRTYGLQAMILGLVGSLVGLML
                     GVLFHQGLPWMITTLTASDLLDQLGFTMNGVELALAPLAKGLALGMLSTLLFALWPLL
                     TIRDVKPAWIFRREIAPLTPTTPHPTAAWWEAWRKLDKVKILVSVAIGLGLALLSVWQ
                     AGSWKVGLLFVLAFAAAVLLLGTSARGVLMALKKWPCPGPLVLRQALGNVVRPGSQAV
                     SITIAIGIAVMVVTTVSLVERSLLTQVGDNRPTDAPTFFFIDIQPD"
     gene            complement(1712..2446)
                     /locus_tag="VN647_02280"
     CDS             complement(1712..2446)
                     /locus_tag="VN647_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG95886.1"
                     /translation="MITVKDVSMALAAASRSVTILDRITFDIPAKQTVAIVGPSGSGK
                     STLLGLMAGLDRPTTGSIQLDGTDITTMGESQMARFRREKVGYIFQSFHLIPTLTAIE
                     NVAVPLELSGVTRTGDRAAELLVAVGLSDRMGHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSSTGSHVIELLLSLHRDYGTTLVLVTHDAVLASSMQRVLSLHDGHLE
                     SDSIPASSKFLGDFCSLAKCKTSTSP"
     gene            2529..3203
                     /locus_tag="VN647_02285"
     CDS             2529..3203
                     /locus_tag="VN647_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWG95887.1"
                     /translation="MKRFHDILVPTFLMLLWPLTSGAISSSASDNRPRIVAFGDSLTA
                     GFGVQADESYPAQLQRRLDNLGYHYRMINAGVSGDTTAGGLRRVPWILNNKPELVILE
                     LGANDGLRGLPVDQTKSNLRQIIRQLQEAGTTVVLAGMKLPPNYGQDYTTNFEAMYQM
                     LAKEYHLALIPFFLEGVGGSSSLNQADGIHPTKEGYEIIVDQVLKVLKPVLNDRLHPS
                     TADKQS"
     gene            complement(3281..3469)
                     /locus_tag="VN647_02290"
     CDS             complement(3281..3469)
                     /locus_tag="VN647_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95888.1"
                     /translation="MKRLLSRTGLLTGTLLVQALPALANAPEGGGPPDYSGITGLYYT
                     LIGVVLAYGVYDTFFKKS"
     gene            complement(3571..4473)
                     /locus_tag="VN647_02295"
     CDS             complement(3571..4473)
                     /locus_tag="VN647_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95889.1"
                     /translation="MQKKIPCSAGRLGLLTSQMIQAISDASAFQIAHQYLTANRVRIA
                     EADDSQITSTVIGNSGLYEQNIRLKDGHLVSKCSCTLPEEPMCRHCLAVLLEYQRWAQ
                     PRQARKPSLAKESITASPVGPSVNGKTSTVQSCSADVTLNEIMVFLEWLQPATKALER
                     QEPLPGPPALSPGPILTWIQAIRNLEDRRRESEEVMTSLESQLKDRESDVGHLTQQLQ
                     TSLREINAAQATSQELQRKVTSYKEVLTRVSELTAEVVQHAGQMRAVTGDLLQKGSQL
                     DKLVSSFKDVAEALQSAVALPPHE"
     gene            complement(4619..5629)
                     /locus_tag="VN647_02300"
     CDS             complement(4619..5629)
                     /locus_tag="VN647_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HWG95890.1"
                     /translation="MRARFLCIDSLGWRSAVMLGLFVSLLAFPIGCAAERDANLRHIL
                     DKGWQPWQILETQTGRVIPFSEFMTNLERYDIVYLGEEHHNPYHIQAALKVLDQLVAD
                     GIEPTIGMEMFGWDGQSALDDYVATPQPVTNEFLEQVRWKQNWGGAFEDYAPLVTFAR
                     DRHLSVRAMNPPKPLIRRVVKLGLDQARQEPEWEPWGILQEDIVDDPAYRERIVDQLR
                     RCHGGSEEHFRTMYEASMVRDEGMARTLVIRQKEFRRENGGPRRMIVSYTGGGHVQYN
                     LPVPKRVAKRLGGDIEQTTIYMTSFEPDKTADVQALIQESIADYIWLTPMGKSGSSKP
                     CR"
     gene            5672..>6571
                     /locus_tag="VN647_02305"
     CDS             5672..>6571
                     /locus_tag="VN647_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95891.1"
                     /translation="MTPKFEDYYAPYPTVLDTLAHTADQPSMSPDPLAEFRRVVSFRA
                     RQFPRQWEASKKLMEGATFPSTIARLCEAIQNKDLPASVKEILLRLFERDTPRRVLDL
                     DGDSLKSVTGLPPAKALRALAVFFGLVPAAVARWPVTHLSSDEVEEAMRHLKNPFDLL
                     CRSDVASVLDIGAGDLSFAEELVDLYGSEPKRQQRQFIVHCLDRLDPCSQLGGPLHAN
                     TERLQKLQRKEGLSFSFFGNQDMFKLGDLDKQEMLAPRYAIATCWAPATPTFAYEPTR
                     LSETLIRSELERTKGTFSQTRFGK"
BASE COUNT         1506 a         1862 c         1773 g         1430 t
ORIGIN      
        1 gatcaggttg gatatcgatg aagaaaaacg tcggggcgtc ggtcggccga ttgtcgccta
       61 cttgtgtgag gagcgagcgc tcgacgagcg atacggtcgt caccaccata acggcgatgc
      121 cgatcgcaat cgtgatgctc accgcctggc taccagggcg tacgacattg cccagcgctt
      181 gacgaaggac gaggggccca gggcatggcc atttcttcag ggccatgagc accccacgtg
      241 ccgaggtacc caacagcagg accgcagccg cgaaggcgag gacaaagagc agtccgactt
      301 tccaagaccc ggcttgccat actgacaaga gcgccaatcc caagccgatc gcgaccgaca
      361 ccaagatctt taccttgtcg agcttacgcc aagcctccca ccatgctgcc gttggatggg
      421 gtgtcgtcgg ggtcagtgga gcaatctcac ggcggaagat ccaagcaggt ttgacatccc
      481 ggatcgtcaa cagcggccac agtgcaaaga gcagcgtgga caacatgccc agtgccaacc
      541 cctttgcaag aggagcgagg gcaagctcca ccccattcat cgtgaatccc aattggtcca
      601 aaagatccga tgccgtcaag gtcgtaatca tccatggaag tccttggtgg aacagcacac
      661 caagcatcag gccgaccaaa cttccgacca gcccaagaat catcgcctgt aacccatagg
      721 tccggataat cgtggaagac tctgcgccga ccgtctttaa aatagcgatc gtattcaact
      781 tttcccgcac aaaggcatgg accgacgtag caaccccaag gcctcctaca aacagtgccg
      841 tcaatccgat caatcctaaa taacgggtca gctgttccaa agactgcttc aattgtggtt
      901 gtgcgtctcg gtaactcgac acgcgggcag aatccgatgc aagttgccct ctcaactcgt
      961 agagcaacgg ttcaggcgcc atcgtgcttg gaattttcag tagatatcgc tcacgaatcc
     1021 tgctacccat cttgaccagt tcagccgtga ggagtccttc tcgtgacatc aggactcgag
     1081 gaccgaggct gaaggcgttg gccatgcgat ccggttccgt cttgactatg ccggtgatga
     1141 tgaacagacc ttgtccgatc ttgagtggat ctcccaccgc gagtcccatc cggatcagga
     1201 gagactcctg caccaccact ccaaaacagg tgcggtctgg acatcggcga gcttgccggt
     1261 caagaaggcc cacgaggccg ccctgtgcct ccaggcggag ggtaccatac agcggatact
     1321 gaggctcgac ggctttgagt tcgatgattt gagtcgactg cccactcccg gacgatccgg
     1381 ctctcgaggc catcgcgacg agctcgctga cgtgggtgag agcgactcca cgatcgctca
     1441 atgagtccag aacttcccgg cctgtggggc tcatcaggcg agagagtcgg atttcaaggt
     1501 cgcctccgag aaggcctctc gcttctttgg tcacggctcg ctctacctgc gttccgaaaa
     1561 gagagacccc ggtcaaggcg cccacgccga tcgctatgca gaccaagaaa tagagaaagt
     1621 gtcgccatgc cgcgcgtgtc tctcgccacg ccatgttgaa catgaaggaa atcatgaccc
     1681 gaatatgggg aaggaatcgg aagatgacgg attacgggct ggtgctcgtt ttgcacttgg
     1741 cgaggctgca gaaatcaccg aggaactttg aagatgcagg tatactgtcg gactccagat
     1801 gtccgtcgtg gagcgacagg acacgctgca tcgatgaggc cagcactgcg tcgtgtgtca
     1861 ccagcacgag ggtggtgcca taatcgcgat ggagcgagag cagcaactcg atgacgtgag
     1921 atccggtaga actgtcgaga ttccccgttg gctcatccgc caataagata ggcggtcgac
     1981 aggcaaaagc ccgggcaacc gcgactcgtt gttgttctcc cccggataac tgaacaggat
     2041 agtgccccat acggtcggac agcccgaccg cgacaagcag ttccgctgcc cgatcgccgg
     2101 tccgagtcac tccgctgagt tccagtggaa cggcgacgtt ttcgattgcc gtaagggtcg
     2161 ggatgagatg aaacgattgg aagatatacc caaccttttc gcgccggaag cgggccatct
     2221 ggctctcacc catggtcgtg atgtccgttc catccagctg aattgatcca gtggtcggcc
     2281 gatcgaggcc ggccatcaaa ccaagcagcg tcgacttgcc acttcctgat ggccccacaa
     2341 tggccacagt ctgcttggcg gggatatcaa aggtaatgcg gtcaaggatc gtgacggaac
     2401 gacttgccgc cgccaaggcc atcgaaacat ctttcacggt gatcatcaaa aacctcattt
     2461 gcctttcgag ataacaggac caggaatgtt attatactgc acagagtata tgatcgagta
     2521 ttgctccggt gaaacgattt catgacatac ttgtgccgac cttcttgatg cttctttggc
     2581 cgctcacgtc gggagcgatc tcttcttcag catcggacaa tagaccacgc atcgtggcat
     2641 ttggtgacag tctcactgcg ggatttgggg tgcaggccga tgaatcctac ccggctcaac
     2701 ttcaacgccg actggacaac ctcggctacc actataggat gatcaacgcc ggtgtgagcg
     2761 gcgacacgac agccggtgga cttcgacgag taccttggat cttaaacaac aagcctgagc
     2821 tggtgattct cgaattgggt gcgaatgacg ggcttcgagg gctcccggtc gatcaaacaa
     2881 aaagcaatct tcgtcagatc attcgacaat tacaggaagc cggtacgacg gttgttttgg
     2941 cggggatgaa gctgccgccg aattacggac aggactatac gacaaatttt gaagccatgt
     3001 accagatgct ggccaaagaa taccaccttg ccttaatccc cttctttctc gaaggggtag
     3061 gcggttcatc ctcgttgaat caggctgacg gcattcatcc gactaaggaa ggctatgaga
     3121 ttatcgtaga ccaagtactc aaagttctca aaccggtctt gaatgatcgg ctccaccctt
     3181 cgactgctga caagcagagc tgaaggcgag acctctcgac cagaacccgc acaatgtaag
     3241 acaaaccaat agaagacggt tcagcgaagc gctctacccc ttacgacttt ttgaaaaacg
     3301 tgtcgtaaac gccgtaggcg aggaccactc cgatcaacgt atagtatagg cctgtgatcc
     3361 cgctgtaatc cggaggccct cccccctctg gggcgttcgc caaggccggt aaagcttgga
     3421 ccagcaaggt tcccgttaac aaacctgttc gtgaaagtag ccgtttcatc tctgccccct
     3481 tccaaatccg cccacgaaaa gtgggttgca ttctactgga cgtcggttgg taggtgcaag
     3541 aggagaatcg gcatagggac gggttagtcg ttattcgtgg ggaggcagtg cgacggctga
     3601 ctggagtgcc tcagccacat ccttaaacga gctgacaagc ttatcgagct gcgagccctt
     3661 ctggagtaga tcgcccgtaa cagcccgcat ctgcccggca tgctgcacga cttcggcagt
     3721 caactcgctg actctggtca gcacttcctt gtaggaggtc actttgcgtt gaagttcctg
     3781 gcttgtggcc tgcgcggcat tgatctctcg cagagaggtt tggagttgtt gcgtgaggtg
     3841 tccaacgtcc gattcacggt ctttcaattg agattcgagg ctcgtcatga cctcttcact
     3901 ttccctacgt cgatcctcaa ggttcctaat cgcttgaatc cacgtcaaga tcggtccagg
     3961 actgagtgcg ggaggaccgg gaagcggctc ttgtctttca agagctttcg tcgcgggttg
     4021 gagccattcc aaaaagacca tgatctcgtt aagagtgaca tcggcagagc acgattgtac
     4081 ggtggacgtc ttcccgttaa ccgaaggacc gaccggagat gccgtgattg attctttcgc
     4141 aaggcttggc tttcgtgctt gccgaggctg ggcccatcgc tgatattcca gcagcaccgc
     4201 gaggcaatgg cgacacatgg gctcttcggg aagcgtacag gaacatttcg agacgaggtg
     4261 gccgtccttc agtcggatgt tctgctcgta cagaccggaa ttaccgatga cggtagacgt
     4321 gatctgggaa tcatcggcct cggcgatacg gacgcgattc gcggtcaagt attgatgggc
     4381 gatttgaaag gccgaggcat ccgaaatggc ctggatcatt tgagaggtca acagtccaag
     4441 ccgcccggcg ctgcaaggaa ttttcttttg catctaaatg taaatgcgcg gatctcttgc
     4501 acaaccacta agcatgatgg cctcagtgtg acgagcgcct tgaatcctgt caagtactca
     4561 acggttttgt gttgccctcg cttgagcgac tgtctgaaga ataaggctct gagacggttt
     4621 accggcaggg tttagacgaa ccggacttac ccatgggtgt cagccaaata tagtccgcga
     4681 tggattcctg aatgagagct tggacatcgg ctgttttatc gggctcaaac gacgtcatgt
     4741 aaattgtggt ctgttcgatg tcccctccca ggcgcttggc gacccgcttc ggcacgggga
     4801 ggttatattg aacatggccc cctcccgtgt aactcacgat catacgacgg gggccaccgt
     4861 tctctcggcg aaattctttt tgcctgatga ccagcgttct cgccataccc tcatcccgga
     4921 ccatcgatgc ctcatacatc gtccggaagt gctcctcgct tcctccatgg caccgtctca
     4981 actgatcgac aatcctctcg cggtacgctg gatcatcgac gatatcctcc tgtaggattc
     5041 cccacggctc ccattcaggc tcttgcctgg cctggtcaag ccccagttta acgacgcgac
     5101 gaatcaaggg tttgggtggg ttcatcgccc ggacggacaa gtgccgatct cgagcgaacg
     5161 tcaccaatgg cgcgtagtct tcaaacgcgc caccccaatt ttgtttccag cgcacctgtt
     5221 ccaaaaactc gttcgtgacc ggttgaggtg tggcgacgta gtcatcgagt gccgattggc
     5281 cgtcccagcc gaacatctcc attccaatgg tgggttcaat gccatccgct accaattgat
     5341 ccagcacttt caaggcggct tggatatgat atggattgtg atgttcttct cccagataga
     5401 caatgtcata ccgttccagg ttcgtcatga attctgaaaa tggaatcaca cgcccggttt
     5461 gagtttcaag aatctgccag ggttgccatc ccttatcgag gatgtgacgc agattggcat
     5521 cacgttcagc cgcacagccg atcgggaacg ccagcaggct gacgaacagg cccaacatca
     5581 cggccgatcg ccaacccaga gaatcgatgc acaggaatcg cgctctcatg aaaccactcc
     5641 taccacacag aataagcatg aacaagccga cttgacgccg aagtttgaag attactatgc
     5701 tccttaccca acagtccttg ataccctcgc acacacagcc gatcagcctt ccatgagtcc
     5761 cgacccactc gctgaattcc gtcgcgtcgt cagttttcgt gcgcgccagt ttccccggca
     5821 atgggaggca tcaaaaaagt tgatggaagg tgccaccttt ccgtccacca tcgcacgact
     5881 gtgcgaggcc attcagaaca aagaccttcc agcttcagtg aaagaaatct tgttgcgcct
     5941 cttcgaacgg gatacgccac ggcgcgttct ggatctcgat ggagacagcc tcaagtccgt
     6001 tacgggcctt cctcctgcca aagccctgcg tgcgcttgcc gtcttttttg ggttggtgcc
     6061 cgcagccgtc gcgaggtggc ctgtgactca tctgtcgagt gatgaagtcg aggaagccat
     6121 gcgccatttg aaaaatcctt tcgacctcct ctgtcgcagt gacgtggcat ctgtgttgga
     6181 catcggggcc ggcgatctct cgttcgccga ggagttagtc gatctgtacg gctctgagcc
     6241 caagcgacaa cagagacagt tcatcgtcca ctgcctggac cgactcgatc cctgttctca
     6301 actaggcggt ccgcttcatg cgaatacgga aagactgcag aagttgcagc ggaaggaggg
     6361 gctatccttc tcgttcttcg gcaatcaaga catgtttaaa ctgggagatc tggataaaca
     6421 ggagatgctg gctccacgat atgccatcgc gacatgttgg gcaccggcca caccgacctt
     6481 cgcctacgaa cccaccaggc tctctgagac gctcattcgc agcgagcttg agcgtaccaa
     6541 aggcacattt agccagacac gttttggcaa a
//
LOCUS       DATNDD010000099         4506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2742999, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000099 DATNDD010000000
VERSION     DATNDD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742999"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VN647_02310"
     CDS             complement(<1..576)
                     /locus_tag="VN647_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWG95892.1"
                     /translation="MATSQKVDHPLEQLLACGQSYWLDNLTQHMIAGVELKGWIQDHG
                     LRGITANPTTFREALTSSDYDRHIESLTHEGRSTKEVYETLLVHDVQQACDLLSEMYR
                     ETDGLDGYVSLEVSPHLAYDTEATMAEARRYVERVNRPNLYIKVPATDAGIPAIEQLL
                     FEGIAVNITLLFSIPSYQAVIEAYISALERRA"
     gene            complement(623..1150)
                     /locus_tag="VN647_02315"
     CDS             complement(623..1150)
                     /locus_tag="VN647_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95893.1"
                     /translation="MRGQHSFLMGVALLALAACQHMGLDGGLGERDGTPTVTRTGEVK
                     DIIIRDDHVSPAIVTANPGDEIRWVNKRQGHARVIFLLPVEGQLSCKRGFGGLMSADW
                     NSFKNQYTAKLGVKDSASVCFKSPSEVKYVVHADSNLLNGEDSIAGTINVGGRQGSSN
                     IEIQGRKISQVSGQQ"
     gene            complement(1857..2411)
                     /locus_tag="VN647_02320"
     CDS             complement(1857..2411)
                     /locus_tag="VN647_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWG95894.1"
                     /translation="MKRASLSLSVALLCIVAGACQRTDVIPEQLEGRVDRDLRYTDIK
                     HNPEAYRGKLMLAGGKVLSAKRTQDGTRIEVLQIPLSEDLIPTGHNAESKGRFVIIDR
                     GEQVSDPAVFDDEKKRVTVVGEVLGMTTIQIDDVKEQVPQLAVKHITVWDWDRMQGGY
                     GPYYGYGYPHAWGYRGYYGYPYYW"
     gene            complement(2722..3468)
                     /locus_tag="VN647_02325"
     CDS             complement(2722..3468)
                     /locus_tag="VN647_02325"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWG95895.1"
                     /translation="MKQFRRVQDITKLTVYGIDGQVGTVQELYFDDQDWTVRYLIVRT
                     GGRLMGRHVLITPIVIEGIDDVDASMRINLQKEQIKHAPSIESTQSISRRYEQTYYER
                     FQWAPYWQPDTTMWGSPIPSPDASTMNLDTPFVSESLEQPHLRSSAAVTGYGIRAQDG
                     EIGHVEDLVIDDEDWIVRYVEVDTRNWLPGKKVLVQTGRIGPFDWYGRSVTLTLTRHA
                     IQSAPTYDPSMLITPDYEVRLFKHYGMGAA"
     gene            complement(3717..4247)
                     /locus_tag="VN647_02330"
     CDS             complement(3717..4247)
                     /locus_tag="VN647_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95896.1"
                     /translation="MAEILVLWMFGLIVIAIGWGVIDKITGPIKAIARSLHSPERSKR
                     LEIVVASLLALAVISIMVGAADNSDNSMGKGTGLPTLLVWAGGLWLIPFRVDAIGDDW
                     LSSLAIAVILVLCWVVTASYLTTRTHAERDISTQQGENTSVTGLGFLFWIFLIGITVA
                     AIARYTSMWNAAALNS"
BASE COUNT         1048 a         1266 c         1086 g         1106 t
ORIGIN      
        1 ggcgcggcgc tcaagcgctg agatgtaggc ttcgatgacg gcctgatagc tggggatcga
       61 gaacaagaga gtgatattga ccgcgatacc ctcgaagagc agttgttcga tggccgggat
      121 cccggcgtcc gtggccggaa ctttgatata gaggttgggc cgattcaccc gctcgacata
      181 tcgtcgagcc tcggccatcg tggcctcggt atcgtatgcg agatgaggag atacctccag
      241 gctcacatat ccgtcgagac cgtcggtctc acgatacatc tcagaaagta agtcgcaggc
      301 ctgctgcaca tcgtggacca ggagtgtttc gtaaacttcc ttcgtggacc gcccttcgtg
      361 tgtgagggat tcaatgtgcc ggtcatagtc actgctcgtg agtgcctcac gaaacgtcgt
      421 gggattggcc gtaattccac ggagtccatg gtcttgaatc catcccttta actcgacgcc
      481 ggcgatcata tgttgagtga gattgtccag ccaatagctc tgtccgcacg ccagaagctg
      541 ttccagtgga tgatcgactt tttgagaggt cgccatatat gcttcctcct ctatctcctc
      601 aaacgaagcc actgtctcat tctcattgct gtccactgac ttgactgatt ttcctgccct
      661 ggatctcgat attcgatgat ccctgccggc caccgacgtt gatcgtgccc gctatactgt
      721 cctctccatt gaggagattg gaatccgcat gcaccacata tttgacttcc gatgggctct
      781 taaaacacac gctcgccgaa tccttgacgc ccaacttggc cgtatactga ttcttaaatg
      841 agttccaatc ggcactcatc aacccgccga aacctcgctt acaggacaac tgtccctcca
      901 ccggcaagag aaagattacg cgcgcatggc cctgccgctt attgacccag cgaatctcgt
      961 cccctggatt ggcagtgact atcgcaggcg acacatggtc atcccgaatg atgatgtcct
     1021 tcacttctcc ggttctcgtg accgtcggtg tcccatctct ttcacccagc cctccatcca
     1081 gccccatgtg ctggcaggcc gccaacgcca ataatgcgac tcccatcagg aagctgtgtt
     1141 gacccctcat tgccgttcct ccatcgttat ggattggatg accatgcgac cgtcaatacc
     1201 ttgccgccac cagcataagt tttcccgata ggcttcacct ttccaacaat cactatctat
     1261 gcgtggagat aaactgacct ggcaatatca attcctacag ctgcgtccca ctgcatagtt
     1321 gtcatttgta tgacctttcc agataaatat tcgaggatac gacacgttct gatctgcaag
     1381 cggagtgctt tctgatactt tgtacccatc atcctgcatg cagctgctcg gaccggacga
     1441 cttgtcctta gattaagaga aaccatccac cttaatcctc gtaatagact actgcggagg
     1501 acggcaaccg tagcttcctg cgtagctggt gaatcggtga atgaagctcc ccgaagccgg
     1561 ttgcgggtat tgcagaacta aaacatatgg agctgtcgga gatcttcgcg gggttgcctc
     1621 agtgccgttc accgggttga ccggttctca tgccctggtc atcaatattc gatggtccct
     1681 ggcggccact gttactgatc gcgtccgcga tatttttctc cccgccaggg ggtatctcat
     1741 cacctttcac atgctgacat gcagtcaaaa gcaacagcgc cgaccccatc atgatatgtt
     1801 gaactttcat tgcgatgttt cctccggtag ataactatta agcgcggctt cgtacactac
     1861 caataatagg gataaccata gtatccacgg tatccccatg catggggata gccgtagcca
     1921 tagtatggac catacccgcc ctgcatgcga tcccaatccc agaccgtgat atgtttgacg
     1981 gccaactgag gtacttgctc cttgacatcg tcgatttgaa tggtagtcat gcccagcact
     2041 tctcccacga cagtcacgcg ttttttctca tcatcaaaca ccgccggatc cgatacctgc
     2101 tcgcctcgat caatgatgac gaaccggcct tttgattcgg cattgtgtcc ggtgggaatc
     2161 aagtcttcgg acaatggaat ctgaagcacc tcgatccgtg tcccgtcttg cgtccgcttg
     2221 gccgacaata ccttgccacc cgccagcata agttttcccc gataggcctc ggggttgtgc
     2281 ttgatatcgg tgtatcggag gtctcgatcg acacgtccct ccaactgctc aggtatcacg
     2341 tccgtgcgct gacaggcgcc ggcaacgatg cataacagag caacgctcag cgaaagactc
     2401 gcgcgcttca tggtatccct cgtatctctt tttgtatcct ctttttttcg accgcctcct
     2461 gtggatgaat aaacctatct tgcaatacct cctgcaatac cgattcctct ggccggatac
     2521 gcttcaggcg tctgataaga actgaaattg ttggattttg tagagaaaac ggccatgcga
     2581 tgaaacggcg cggcccggga ctttctgaag gaacggacaa cttgtcctcg atagggaaaa
     2641 ctatttcaat tgtatggttc aatgagacga cttcggccag ccgctaccga tgactcaatg
     2701 gaaatggagc cggcttcgtc gttacgccgc gcccattcca taatgtttga acagccgaac
     2761 ctcgtaatca ggtgtaatga gcatagacgg atcataggtc ggcgccgatt ggatggcgtg
     2821 cctagtcaag gtcaaggtaa ccgatcggcc ataccagtcg aatggtccaa ttcggcctgt
     2881 ctgcaccagt accttcttgc cgggcaacca gttccgcgta tcgacttcaa catatcgaac
     2941 gatccaatct tcatcgtcga tgacgagatc ctcgacatga ccgatttctc cgtcttgcgc
     3001 acgaattcca tagccggtca cggcggcgct gctgcgaaga tggggctgtt cgagggattc
     3061 cgaaacaaaa ggcgtatcca ggttcattgt cgaagcatca ggagagggaa tcgggcttcc
     3121 ccacatcgtg gtgtccggct gccagtaggg cgcccactga aaacgctcat aataagtttg
     3181 ttcgtaccgg cgtgaaattg attgggtact ctcgatcgaa ggagcgtgct tgatctgctc
     3241 cttttgcaga ttgatccgca tggaggcgtc gacatcatcg atcccttcaa tcacaatcgg
     3301 cgtgatcagc acatgacgac ccatgagccg tcctcccgtc ctcactatca gataccggac
     3361 cgtccagtcc tgatcatcga aatagagttc ttgtaccgtt cccacttgac cgtcgatacc
     3421 ataaacggtc aatttggtga tgtcctgcac ccgcctgaat tgcttcatac ctgctccaaa
     3481 acttgatggc ggccatggta gccgttcacc ctacaccgtt ccactaggga aatcccttgt
     3541 ttcatccagc tggagtaatg aggcgattga ttcctaatgg gattcgtgaa cggcgtgtca
     3601 caggtggagc agataacgcg atagctacac cctcggcaaa ctcgattttg agatatattc
     3661 cgactagttg ttgtcaggta agggttttgt cgactaacct aggtagggcg cacggttcat
     3721 gagttaagag cggcggcatt ccacatagat gtgtagcggg caatagcggc aacagtgata
     3781 ccgatcaaga aaatccaaaa gaggaaccca aggccggtca cgctcgtatt ctctccctgc
     3841 tgagtcgaga tatccctctc ggcatgcgtt cgtgttgtaa gataagaagc ggtcaccacc
     3901 cagcatagta ccagaataac ggctatggcc agggaagaga gccagtcatc ccctatagca
     3961 tcaacccgaa atgggatcag ccagaggccc ccggcccata ccaagagcgt cggcaagcct
     4021 gtcccctttc ccatactatt atcactatta tccgcagcgc cgaccatgat cgagatgacg
     4081 gctaacgcta atagactcgc gactacgatc tccaaacgtt tgcttcgctc cggactgtgc
     4141 aacgatcgag caatggcttt aataggaccg gtgattttat caatgactcc ccatccgatg
     4201 gcaatcacga tcagtccaaa catccacaat acaagaatct ctgccatgga aaacctctcg
     4261 atgtatctat gattgtgatg ttatgcaaaa cgcgcgccat gtcataatgg catggcataa
     4321 atcattcgcc tagtctttgt tgagattcat caccagcgga tgggaaaaac tcgattgacg
     4381 tcaaaaatat ggcggggagt ttcggtcaag actgacgccg gaataatctc aggatgctca
     4441 aaaaaggctg ttgatctctc ccgccgaccg tcggcacgcg atcgctatcg aaactctttg
     4501 gaccag
//
LOCUS       DATNDD010000100         4047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_947158, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000100 DATNDD010000000
VERSION     DATNDD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947158"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VN647_02335"
     CDS             <1..690
                     /locus_tag="VN647_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95897.1"
                     /translation="GVSPHKCFRGKIAEIGQMTLLIRAHNGRKALLALIQKVKGIIEH
                     KVVAAAESKRIHVEGRTDTLIELSRPFSKFGKLSQIIRAVRKLTDYPARLLKYIDVGC
                     EIRWRLRSIHTLSINIEAVTQSASYAGCVLQHDVLMPVIIDIELRCVGTPTKVVEHER
                     PIDGSEFFVLIVFQFTKVLHILIKEIQHVLTHGCGLPHDQGVRLSQSKRRHKKRQGHT
                     VDDAAFESPHM"
     gene            complement(725..1045)
                     /locus_tag="VN647_02340"
     CDS             complement(725..1045)
                     /locus_tag="VN647_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95898.1"
                     /translation="MSEPKDTGILNVRLKPSESSAHPRAANYSNVGIAQGIAYLDFGF
                     IEPALLAEIAKTAKEGQAAPKGLDGHLVSRVAMDVGALARLQKQIRQVLVSIHETRRG
                     KAKG"
     gene            1516..1770
                     /locus_tag="VN647_02345"
     CDS             1516..1770
                     /locus_tag="VN647_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95899.1"
                     /translation="MIPHLYYQIGMPVVCLASAVLGFLEPRKSWRWGALPIYGQFFWL
                     LITQGPGNLLPLGMIAFSILAIPSIISARIGAFLGRKRAA"
     gene            1853..2182
                     /locus_tag="VN647_02350"
     CDS             1853..2182
                     /locus_tag="VN647_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95900.1"
                     /translation="MSELPSQQSFKLLPAYLGTTSIEEVVQTARGRRVLWLEILLNDR
                     LDLTPWQSEPAVQEAYRTACRWYTHYRRLLTYLFDRVPLPTDSGPIDFREYRTFAEAV
                     YFAYAHR"
     gene            2163..2693
                     /locus_tag="VN647_02355"
     CDS             2163..2693
                     /locus_tag="VN647_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HWG95901.1"
                     /translation="MPTLTAEHLIELLTRYVADSDTPVDLLLVGALALHAYGVPARVT
                     HDVDAELQGPLMPLRDYLMSKNIPADLTQNFSGWSVVAMPPGYRDRAVALINQPRLRI
                     RVLSPVDFVIAKLRRGTELDLDDALLVARQHHLSTGIIRESAREALAASPQDTALFLF
                     QKTVDFFCKNLISSND"
     gene            2905..3441
                     /locus_tag="VN647_02360"
     CDS             2905..3441
                     /locus_tag="VN647_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95902.1"
                     /translation="MDFVSHGLWGSIAFGRKNRSSFWLAFAIGMAPDLFSFGILYVAA
                     AFGLSPKPDFSHGTPPESTIPQYVHHLYHYTHSFVIFLAVFFLVWLSLKRPVWELGAW
                     GFHVLADVPLHSYAFFPTPVLWPLSDWKFDGWQWMTPGILITNFALLSLLYVWYIRQL
                     FLARKKEEARPETISRAT"
     gene            3796..>4047
                     /locus_tag="VN647_02365"
     CDS             3796..>4047
                     /locus_tag="VN647_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95903.1"
                     /translation="MRYISIIGGVVVALAFAATATAGSFEDKAQVTVSGAEYMLNGYI
                     VKVEGNAYWIRRASGEVVRVAVTEDTHLICPTRTGNKAAK"
BASE COUNT          880 a         1132 c         1019 g         1016 t
ORIGIN      
        1 ggcgtctctc ctcataaatg ttttcgtggc aaaatagcag agatcggtca gatgaccctg
       61 cttatccgtg cgcacaatgg ccgtaaagcg cttctggccc ttattcaaaa agtgaaaggg
      121 atcatcgagc acaaggtagt agcggccgcc gaatcgaaac ggattcatgt ggaagggcgc
      181 accgatacgc tgattgaact cagccgtccc ttctcgaaat ttgggaagct ttcacaaatc
      241 atacgtgcgg tgaggaaatt gaccgattac cccgctcgcc tgcttaagta catcgacgtt
      301 ggatgtgaaa taaggtggag acttcggagc attcataccc tcagcataaa tatagaggct
      361 gtcactcagt ctgcctcgta tgcaggctgc gtgcttcagc acgacgtcct catgcccgtc
      421 attatcgata tcgaactgcg ctgtgtgggt acgccaacaa aagtcgtcga gcacgagcgt
      481 ccaatcgatg gaagtgaatt cttcgtgctg atcgtatttc aattcaccaa agttcttcat
      541 atcctcatta aagagattca gcatgtgctg acacacggct gcggccttcc ccatgaccaa
      601 ggtgtacgac tcagccagag caagcgtcgg cataagaaaa gacaaggtca tactgtagat
      661 gatgctgcat ttgagtcgcc gcatatgtag gtctcccctt ttcatcacct gccaccctca
      721 gcctctaacc cttcgccttc ccccgccgcg tctcgtgtat gctcaccaat acctgccgga
      781 tttgcttctg cagccgcgcc aacgcgccaa catccattgc tacgcggctg accagatggc
      841 cgtctaatcc cttcggcgcc gcttgtccct cctttgcggt tttcgcgatt tccgccagca
      901 acgccggctc gataaagccg aaatccagat acgcaatgcc ttgcgcgatt ccaacattcg
      961 aatagttcgc cgcgcgcggg tgggctgacg attcagaggg ctttaaccga acgttcaaga
     1021 tgccggtatc tttcggttca ctcatcgcga gagactcctt agtaagaatt tgatgcatcc
     1081 tgctgatggc ttggttcgag aatatggctg ttcatatttg cgatccagtc aatttactga
     1141 agtgtctgaa gctcatagtt gcgtagcctg cacctttgta gcaaacaatc gcaaaacggt
     1201 agtttggtct ctgggtacct attctcgata tgggaagaca ggctccgctc ggaatcggcc
     1261 ttgcctaata aactcatcaa caaagatcac cagtggtttc gctaccttgt tcatcaaaac
     1321 atcgtgtttg aaagcttcat aaccgttcgc gattctatta agagagagta aactccgagg
     1381 aatacaactt agcggcctct tcgcaggctg aacggtttcg catgaaacat atccattggc
     1441 tgtatggaac agctgtgact atcggaattg tgatgtgggt gggcatctcg atggtttccg
     1501 gaaggcgaga ggcctttgat tccccatttg tactatcaga ttggcatgcc ggtggtttgc
     1561 ctcgcttctg ccgtactggg ctttcttgaa ccgcgcaagt cgtggcgatg gggcgccctt
     1621 cccatttacg gccagttttt ctggctgctg atcacccaag ggccaggcaa tctcttgccg
     1681 cttggcatga ttgctttctc catccttgcc ataccctcga tcatctccgc gcggatcggc
     1741 gcattcctcg gaagaaagcg tgcagcataa ccaggctacg actttcaaga gaaacccgac
     1801 cagttagacg gtttggatat ggattgggat acacctgtgt tacactagcg ccatgtcgga
     1861 gctgccttca caacagtcgt tcaaactact gccggcgtat cttggcacaa cgtcgattga
     1921 ggaagttgtt caaaccgcaa ggggccgtcg ggtactctgg ttggaaattt tattgaatga
     1981 ccgattggat ctgacgcctt ggcaatccga accggcggtc caggaagcgt acaggaccgc
     2041 ctgtcgatgg tatacgcact accgccgcct gctgacctat ctctttgacc gtgttcccct
     2101 ccccaccgac tccggtccga tcgacttccg tgagtatcga acgttcgccg aggcggtcta
     2161 ctttgcctac gctcaccgct gagcatctca tcgagctcct cacccgctac gttgccgact
     2221 ccgacactcc tgtagacctc ctcctcgttg gcgcgttagc cctgcacgcc tacggagtgc
     2281 cggcccgggt cactcacgat gtcgacgccg aattacaagg tccgctgatg ccgctgagag
     2341 attatctgat gtccaagaac attccggctg atctgaccca aaatttttcc ggttggtcgg
     2401 tagtcgccat gcctccagga taccgggacc gtgccgtcgc tctcatcaac caacctcgtc
     2461 tgcgtatccg tgtcctgtcg cccgtcgatt tcgtgattgc caagcttcgc cgtggaacag
     2521 agctggatct tgacgatgca ttgctggtgg ctcgacagca ccacctatcc accggaatca
     2581 ttcgggagag cgctcgggaa gctctcgccg cctcaccgca agacaccgcc ctctttctct
     2641 ttcagaaaac ggtcgatttc ttctgcaaga atctcatttc atcaaacgat taaagaaata
     2701 gttcagaaga caaagggaga gcctgcgtgg agcaggtttc cccataccag tgtcgagagt
     2761 tcattttgag acggcgcgtt tgcagtctgc gtcctctccc ctctgggtaa atcgaacgct
     2821 ggttcgcttt cggacaatac cgcaatggac cggagcgaac aggaccaagt agaatatcgc
     2881 cggttcacct cgtgagagaa tctgatggat ttcgtatcac acggcctctg gggttcgatt
     2941 gcattcggac gtaagaaccg atcgagtttc tggctggcat tcgccatcgg gatggcacct
     3001 gatctgtttt cattcgggat cctgtacgtg gccgccgcat tcggcttatc gccgaagccc
     3061 gatttcagtc atggcactcc tccggaatca accattcctc aatatgtgca tcacctctat
     3121 cattacactc atagcttcgt catatttctc gccgtctttt tcctcgtatg gctttcccta
     3181 aaacgaccgg tttgggaact gggagcgtgg ggattccatg tcctggccga cgtgccgttg
     3241 cattcgtacg ccttttttcc gacgccggta ctgtggcccc tctctgactg gaaattcgac
     3301 ggctggcaat ggatgacgcc gggtattctc atcaccaact tcgcgctgct gtcgctgctc
     3361 tatgtctggt acatccggca gttgttcctg gctaggaaga aagaggaggc tcggccagaa
     3421 accatttcgc gggccacatg aatgacaatt cacaggatct cgcaacatta cccggacggt
     3481 atatccctat tttctgagat gcctgatgcg tcgcttgcat catcgtcaac agaagagtag
     3541 gctgtatgta atcgaccctt tcgcaagggg gatgcgatgt cggctcccct cctcttgcgt
     3601 tttctagttt tcccctccgc ctcggcccga tacctcagtg atctgatcgc caatttattt
     3661 gattcaacct cggcatttca ttcccatcaa tcaatgatgt ttcacgcctt ggatgagtct
     3721 agacgtgcga cagcagacgg agaacatggt gccagcagat tcagcaagat aacctctagc
     3781 aaggaggatt gctccatgcg ttacatcagc atcataggag gagtcgttgt cgcgctcgcc
     3841 ttcgctgcga cagcgactgc cgggtcgttt gaggataagg cgcaagtgac ggttagtggt
     3901 gccgaataca tgttgaacgg ctatatcgtc aaggtcgaag ggaatgctta ctggatcagg
     3961 agggcgtcgg gcgaggtcgt tcgcgtcgca gtcacggaag acactcacct gatctgcccg
     4021 actcgcaccg gcaacaaggc ggcgaaa
//
LOCUS       DATNDD010000101         4990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2664757, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000101 DATNDD010000000
VERSION     DATNDD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2664757"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VN647_02370"
     CDS             complement(<1..295)
                     /locus_tag="VN647_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HWG95904.1"
                     /translation="MNRGIYPILSGALAHERRMQVFANNMANVNTAGFKQDEQAFKTI
                     FPRYHAVIPVGSPTAGLAQQIMARPFGPTERAFVAPHQIKTTFEPGRIRLTGNP"
     gene            557..694
                     /locus_tag="VN647_02375"
     CDS             557..694
                     /locus_tag="VN647_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95905.1"
                     /translation="MAALHLEGEHLADFWLREKPTLVPTMELDPSRFIAEQDLRPHFY
                     T"
     gene            complement(823..1551)
                     /locus_tag="VN647_02380"
     CDS             complement(823..1551)
                     /locus_tag="VN647_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWG95906.1"
                     /translation="MEAIQVGPPSVADVLPLRVVIGWMGLFIAAGFGVAGLLCWPYGQ
                     DLVRRKQDGAPSAYDGVTGLPTRRLFLVLLKQALSRAETTKRHVAVLVCVLEQFRPLS
                     TSSVAPNMTLVVRVQAARIKSALRPHDVVARLDEYTFAVIADNLESPDEARLIAGKIQ
                     QAIALPLLIEGQELLISCRIGGIVGPQSGTNAEMLLEAASETLSNSPTINDTVVLVPD
                     PATSFSSSNQASAGRIRHYADARH"
     gene            complement(1887..2636)
                     /locus_tag="VN647_02385"
     CDS             complement(1887..2636)
                     /locus_tag="VN647_02385"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HWG95907.1"
                     /translation="MSKTASPHEQESFSAQGVRREQLIKEFAHVIRAMAHRLAFRLPA
                     YLDAEDLISVGTIGLMDAMDKYDPTREAKFKTYAEFRIRGAMLDEIRSMDWIPRSVHE
                     RIGLLQKTHTVLMSRLGRPPQDEEVAEELKMSMEELDDFVMRARGAVMISVDDLSLHE
                     PDGHKVVKMLTDTHTPDPLSSLVNERERASLAGAIQSLPEKERLVLTLYYYEELTMKE
                     IGELLKVTESRVCQIHTKAIIRLKAHLQKPN"
     gene            complement(2661..3557)
                     /locus_tag="VN647_02390"
     CDS             complement(2661..3557)
                     /locus_tag="VN647_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HWG95908.1"
                     /translation="MDNPVGGRDMSFRTRKSVLASEGLSRDGGSSTLVIAVSSGKGGV
                     GKTNVVANLAVALTRVGKRVLILDADLGLGNLDVLLGLVPRHTIEDVLVGTHTLDEIV
                     LKGPAGIHVLPASSGVPRLTALTEAQQLMIQEQLAQLTSEMDVLLIDTGAGIAPNVTF
                     FAAAADETMIVVSPEPTSLTDAYALIKVLARQYRERRFKVLVNQAKSPREAAEVFGKL
                     DVAVDHFLHVAVELVGAIPYDDYMHLAVMQQKALSELFPEAPAAQAFKRLAQQILQWP
                     RPGLPKSSIQLVWQRAATPAGN"
     gene            complement(3550..4890)
                     /gene="flhF"
                     /locus_tag="VN647_02395"
     CDS             complement(3550..4890)
                     /gene="flhF"
                     /locus_tag="VN647_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HWG95909.1"
                     /translation="MKVKTFHALTMQDAMRDIKEELGPDAIILSAKEVREGGRPLKAF
                     NRPVLEVMAASDQDGQRFPQGREVQQVSPAKPSDAGADSAPSAQSFQQTLHGMLKPDC
                     ETMTPTVGKRTALLKPAAVHGKPNRLRHLHTVVNELGRLLEDLSRADGRTIGSLPSPM
                     PVWLRRSLIEQGMNPSTVDLLLNEAGKTEQVSGPRDDESMRRALHHEIVKRVRTSGPP
                     LRGEGYPSIGLLIGPSGAGKTASVAKLASHYRLEQRRSVALITFDTCRETAVEQLRRY
                     AKVVGVPFACALSARQVHEGLRRHTQVDLVLIDMPGIGPRDLALAQELHRLLPKDAVT
                     THLVLPASTREQELCRITRCLRDLPQPRLLFTKLDETESFGTIFEVAHQTGVPLSYWS
                     IGRRVPGDIEVASSERLAAFLTAERSTHSQVFVNRSIQSSEAASALLGAGTHHG"
BASE COUNT         1089 a         1441 c         1389 g         1071 t
ORIGIN      
        1 gcggattgcc ggtcagtcga atgcgacccg gctcaaatgt ggtcttgatt tggtgcggcg
       61 cgacaaacgc tcgctcagtc ggcccaaacg gcctagccat gatttgttgg gccaatcccg
      121 cagttggact gccgaccgga atcactgcat gatatcgagg aaagatggtt ttgaaggcct
      181 gttcgtcctg cttgaagccg gcggtattca cattggccat gttgttcgcg aagacctgca
      241 tccgacgttc atgagccagc gctccggata agataggata gataccccga ttcatactgc
      301 cagctccttt tgctttcgag aaagagacag caaccctcat gccaggttcg ccgacgagag
      361 actggtcgga taagtaccaa ggaacgattg gaaataagcc aattccttaa gagatttagg
      421 gaactgacta ctgatggaag aagaaatgct gggaagtcgc cgtcaggtga caggataacg
      481 ctaggccatt cctaccaggc ccagagaaaa atattcctag tgggtttaca aacaactcgt
      541 caggtgagga gaagtcatgg ccgccttgca tctggagggg gagcacctgg ccgatttttg
      601 gttaagagag aaacccacct tggtgccgac catggaactg gaccccagtc gcttcattgc
      661 agaacaggac ctgcgtccac acttctacac gtgataagtc tcagccgtgc tgcatgtaag
      721 agggcagctt cctgaccgtg cgtaatagtc cagatgcaca tcggtgcggg acggtaggct
      781 atgagatctg gcgtgactat tggcttatta ttcgcaatga ggctaatgcc gcgcgtccgc
      841 gtagtgacgt attcggcctg cagaagcttg gttcgaggaa gagaaagacg tggcgggatc
      901 cggcactaac actacggtgt cattgatggt tggactattc gagagagttt ctgaggcggc
      961 ctcaagcagc atttcagcat tagtcccact ttgtggcccc acgattcctc caattcggca
     1021 ggaaatcagc aactcctggc cttcgatcaa cagtggaagt gcgatcgcct gctggatttt
     1081 cccggcgatc aatcgagctt cgtccggtga ttcgagattg tcggcgatca cggcgaaggt
     1141 gtattcgtcc aagcgtgcca ccacgtcgtg cggtcgcaat gcgctcttga tgcgggcagc
     1201 ttgcactcgc acgaccaagg tcatattcgg tgcgactgag gacgtcgaca gcggacgaaa
     1261 ctgttccagg acacatacca agacagcaac gtgccgttta gtcgtttcag ccctgctcaa
     1321 cgcctgtttg agaagtacaa ggaaaagccg ccgggttgga agaccggtca caccatcgta
     1381 cgccgacggc gcaccatcct gcttgcgccg tacgaggtcc tgaccatacg gccaacacaa
     1441 gaggcccgcc acaccgaatc cggcagcaat gaaaagcccc atccatccga tgacaactcg
     1501 cagagggaga acgtcggcca cgctcggagg accgacctgt atcgcctcca tccacaccat
     1561 acttgcaccg caaaccgttg ctgagacaca ccaccatgct agagagcgcg gtcgcgatcg
     1621 tgaggaccca gcgtgtgata ccttccagtg catcgtcgtg attactgatt gaagatcgac
     1681 ttgagttcgg aatagacctg agacccgatc acgttccggc ccgatgcgat gaattaccac
     1741 tcaatccacc aggtccttgt cgaggtctcg tccagtaagg tatcggtttc tccgactcag
     1801 aactgaaggt gggcagcgca caatacctcg aatggcatca gaacgaatac aaatgaacgg
     1861 ccgactcagt cggtcggccg attcgatcaa ttcggttttt gcaagtgtgc cttgagcctg
     1921 atgatggctt tggtgtggat ttggcagacc cgtgactcag tgactttcag cagctcgccg
     1981 atttccttca tcgtgagttc ctcgtagtaa tacaacgtca ggacgagccg ttctttttcc
     2041 ggcaacgatt gaatggcccc ggcaagcgat gcacgctcac gctcgttgac caaagaggaa
     2101 agaggatcag gcgtgtgcgt atcggtcaac atcttgacca ctttgtgccc gtccggctca
     2161 tgaagactta aatcgtccac gctgatcatc accgcccctc tggcccgcat aacgaaatca
     2221 tccagctctt ccatggacat cttcagttct tccgccacct cttcatcctg cggcggtcgc
     2281 cccagtcggc tcatgaggac cgtgtgagtc ttttggagca ggccgattcg ttcatgaacc
     2341 gatcgtggga tccaatccat cgaacgaatc tcatcgagca tggctccacg gattctgaac
     2401 tccgcatatg ttttgaactt cgcttctcgt gtcgggtcat acttgtccat ggcatccatg
     2461 agtccgatgg tgccgaccga aatcaggtcc tctgcgtcca agtacgccgg gaggcggaag
     2521 gccagccggt gggccatggc gcgaatgaca tgggcaaact ccttgatgag ttgttcgcgc
     2581 ctgactccct gggccgaaaa agattcttgc tcgtgcggtg aggctgtctt gctcatgatc
     2641 ttcccatgta cgatcgtgtt tcagttgccc gccggcgttg ccgctcgttg ccagacgagc
     2701 tgtatggaac ttttggggag accaggcctc ggccactgga gtatttgctg cgctaaccgc
     2761 ttgaatgctt gggccgccgg tgcctccggg aacaattcag agagcgcctt ctgttgcatg
     2821 accgccaaat gcatatagtc gtcataggga atcgccccga ccaattcgac ggccacatga
     2881 agaaaatgat ccaccgccac gtccaactta ccgaatactt cggcggcttc gcggggactc
     2941 ttggcctgat tcaccagcac tttgaaacga cgctcccgat actggcgagc caagactttg
     3001 atcagtgcgt aggcgtccgt gagcgatgtg ggttccggcg agacgacgat catcgtctca
     3061 tccgccgcgg cggcaaagaa ggtcacgttc ggcgcgatgc ctgcgccggt atcgatcaac
     3121 aacacatcca tctccgaggt gagctgcgcc agctgttcct ggatcatcag ctgttgagct
     3181 tccgtcagtg ccgtcaggcg tgggacgccg gaactggccg ggagcacatg aatgcccgcc
     3241 ggacctttga gcacgatctc gtcgagagta tgtgtgccga ccaatacgtc ttcgatcgta
     3301 tgccgtggaa ctagtcccag caggacatcg agattgccta agccgaggtc ggcgtccaaa
     3361 atcagcaccc gctttccaac cctcgtgagg gcaacggcaa gatttgcgac cacattggtc
     3421 ttcccgactc ctcccttgcc gctggagacg gctatgacaa gcgtggacga gccaccatcc
     3481 cgactcagac cctccgacgc aaggacagac tttcttgttc taaacgacat atcacgacct
     3541 ccaaccgggt tatccatggt gagttcctgc tcccagtaat gcagaagctg cttccgatga
     3601 ttgaatcgac cgattgacaa acacctgaga atgggtggag cgttccgcag tgagaaacgc
     3661 cgccaaacgc tccgacgagg ctacctcgat atctccgggg actcgccgtc cgatactcca
     3721 ataggaaagc ggcacgccgg tttggtgggc cacttcaaag atggtgccga acgattccgt
     3781 ctcgtcgagc ttggtgaaga gcagccgcgg ttgcggcaga tcacggaggc atctcgtgat
     3841 acgacagagc tcctgctccc tcgtggaagc cggaaggact agatgtgtcg tgacggcatc
     3901 ctttggtaga agcctgtgca gctcctgggc caatgctagg tcccgcggcc cgatcccagg
     3961 catgtcgatc agcacgaggt ctacctgtgt atggcggcgg agcccttcat ggacctgtcg
     4021 agccgacagg gcgcaagcga acggcactcc aacaaccttg gcataccgac gcagctgttc
     4081 aaccgcggtt tcccgacagg tatcaaaggt aataagggcg acggatctcc gctgttcaag
     4141 acgatagtgt gaagccaact tggctacaga ggcggtcttc ccggctccac tgggtccgat
     4201 caggagaccg atagaaggat agccctcccc tctgagcgga ggcccgctcg ttcgcacgcg
     4261 cttgacgatt tcatgatgca gcgcgcgccg catcgactca tcgtcgcgtg gaccggagac
     4321 ctgttcggtt ttgcccgcct cgttcaagag caagtccacg gtggagggat tcattccctg
     4381 ctcgatgagc gagcggcgca gccacaccgg catgggcgac ggcagacttc cgatcgttcg
     4441 gccgtcagcc cgcgacaggt cttccaataa gcgacccaat tcattcacga cggtgtgcag
     4501 atgccgaagg cgattcggtt tcccatgcac tgcggccggc ttcaacaagg cagtccgctt
     4561 accgacggtc ggtgtcatcg tctcgcaatc aggcttcaac atcccgtgga gtgtctgttg
     4621 gaaggactgc gcggacggcg cggaatccgc cccggcatcg gacggtttcg caggagaaac
     4681 ttgttgcacc tccctaccct gagggaaccg ttgtccgtct tgatcggagg cggccatcac
     4741 ttccagaacc ggtcgattga atgccttcag gggacgccct ccctcgcgca cttctttcgc
     4801 cgataagatg atggcgtccg gccccagttc ttctttgatg tcgcgcatgg catcctgcat
     4861 ggtgagcgcg tgaaatgtct tgaccttcat ggcttacccc aagacgtctg cgccaattcc
     4921 aagtcgatgc gtaccgtatc caacgactgc agacggacga aggaatccac ttcattgagt
     4981 cccatcacgg
//
LOCUS       DATNDD010000102         4028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_376624, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000102 DATNDD010000000
VERSION     DATNDD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376624"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..670)
                     /locus_tag="VN647_02400"
     CDS             complement(188..670)
                     /locus_tag="VN647_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95910.1"
                     /translation="MKIQSVRCNNRKKAFQVKISKGMLSFPYSKTEPRPSSKDPIITV
                     WVDRELGGDGFTYVLESGKEGTVHSEQVLEYNQDPHYLRDILLYKLTIEAQKRVKGSP
                     LSKREIIRRLSTSATQFYRLLDQTNYRKSIDQLLSLLHVLDCEVELQVRVKTLSRKVA
                     "
     gene            1167..1643
                     /locus_tag="VN647_02405"
     CDS             1167..1643
                     /locus_tag="VN647_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95911.1"
                     /translation="MVFLFGALGCSSATYIQAKVTENPIDRVVDQSGEIWSKKRIDSN
                     TLELSQPWPTSGFGRVLFFWDLDRNVTHANLSYDATASVLNIQYYLEGHQLLMLFIPF
                     SVDAEPAVVVGRAVKPAMNAQIQDILRWSGASVLSRRGGDKSEQFPPHTTSASPPN"
     gene            complement(1726..1941)
                     /locus_tag="VN647_02410"
     CDS             complement(1726..1941)
                     /locus_tag="VN647_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95912.1"
                     /translation="MIIYPAGRYLTTSAVKDQLQTRNLFSGVDHDFGRAVFTEDDIGS
                     QLASMNLTRLLIDIPDDRHWVVGGIIY"
     gene            1940..2146
                     /locus_tag="VN647_02415"
     CDS             1940..2146
                     /locus_tag="VN647_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95913.1"
                     /translation="MTSVHEQAFLEKLRHLPPEQVVEVEHFVDFLVQRGAEEHRLTQA
                     ASQVAVPAFARIWDNPDDADYDRL"
     gene            2133..2471
                     /locus_tag="VN647_02420"
     CDS             2133..2471
                     /locus_tag="VN647_02420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWG95914.1"
                     /translation="MTGYEFGEIVLVPFPFTDQSATKHRPAVVISSTAYHRARPDLII
                     MAVTSQQPTSDALGEVVVEDWRAAGLLKPSVLKPVLTTIDPTLILKKLGRLTTSDQVH
                     LRQALAQIIG"
     gene            complement(2540..3199)
                     /locus_tag="VN647_02425"
     CDS             complement(2540..3199)
                     /locus_tag="VN647_02425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG95915.1"
                     /translation="MDRVLEPELMDDRKQAEAYARADFAKENQGFVNRFKEYFPEFAQ
                     GMVLDLGCGPADIPIRFAKLYPACQVIGIDASAPMIQLGEQAVKQADLTDRITLRCER
                     YEEVAGARIVDAVISNSLLHHLPNPLQFWQKLRQLVKPGAPVLVMDLLRSESPEAAQA
                     IVDQYAANESDILRRDFYNSLLAAFTEDEIGSQLARMNLTRLLIDIPDDRHWVVGGII
                     Y"
     gene            complement(3283..3765)
                     /locus_tag="VN647_02430"
     CDS             complement(3283..3765)
                     /locus_tag="VN647_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWG95916.1"
                     /translation="MSTGPPYPHVHQINVSDGGVPKLPVWEAKVSERGLDGDRQRNLK
                     FHGGPDRAVCLFSLELIERLQDEGHPINVGSSGENLTLSGLEWELMRPGVRLTIGPEI
                     QLEVTSYTTPCIHNKSWFRDEDFSRISQKLNPGWSRVYAKVLQGGVVRPGDAVTIEKV
                     "
     gene            complement(3768..>4028)
                     /locus_tag="VN647_02435"
     CDS             complement(3768..>4028)
                     /locus_tag="VN647_02435"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWG95917.1"
                     /translation="LPEEKRRIADLVLANLDRQTILDLADDLACSALRRARIMVSGIL
                     VEQEDEIVERFSNLGLVCSERCEDEGWVAMKFFRPESCNGEA"
BASE COUNT          966 a         1137 c          954 g          971 t
ORIGIN      
        1 agctcaaccc tgggtcgtca tggagatgcg ccctcaccgg ccagctgagc cacacgaacc
       61 agctggtcgc agcacgaaag gtgttccgtg ggatcttgcc ctgtgcgttt gtcgtcttcc
      121 ttcctattgg catgcacaaa acaagatgtg accccagctc taccctctgc cagcgaacac
      181 ttgagagcta agcaactttt cgggacaagg tcttaactcg aacctggagt tccacttcac
      241 aatccaacac atggagcaat gaaagaagtt gatcaataga tttcctgtag ttggtttgat
      301 cgagtaggcg ataaaactgc gtggctgatg tgctgagccg tcgaataatc tcgcgctttg
      361 ataaaggact tcctttgact cgcttctgcg cctcgatcgt cagcttatat agcaagatat
      421 cccgcaagta atgtggatcc tggttatatt cgagcacctg ttcgctatga accgtccctt
      481 ccttgccgga ttccagcaca taggtgaacc catcccctcc cagctctcta tccacccata
      541 cggtgatgat cggatccttg gaacttggtc gaggctcggt cttcgaatag ggaaacgaaa
      601 gcattccttt cgatatcttt acctgaaatg ccttcttacg attgttacaa cgtactgatt
      661 gaattttcat atctctccct cgaacgctaa ctgttcaatc aactcgcgga ctcgtcatgt
      721 cacctttcct ctcatcagta cgttattgtc caagtcccat ttcacaacga acctgccatt
      781 ccgatagaca tgcacatgcc ggggagagtg atcgcctttc catgtaacaa acacatagcc
      841 gccccggcga accttcccca tacagtagtg ttacttttat aggtaacgct tgtcaacttg
      901 ttcaccgcac agaaaaagtc gtgcccctag ctaaacggtt cgaacagaat ccgcttgttc
      961 acgccgatcc acttgaacaa tccgagttgg gctcctttcg gtttcgcatc ggagaatacc
     1021 gtgtcgccct gaatctgaag agtgttggcc tgttattgaa ataactggcc caacacttag
     1081 cttgagaata tcctataaac cagcatttgt aggaggcatc cgcgatgcat catcaatctg
     1141 cagctttatt tttatggata ggatccttgg ttttcttgtt cggggccctt gggtgttcat
     1201 cggctacgta tatccaggcg aaggtgacgg agaaccccat cgatcgagtt gtagatcaat
     1261 cgggagaaat atggtctaaa aagagaatcg attccaacac cttggaactg agtcagccat
     1321 ggccaacttc tggctttgga agggtactct tcttttggga tttagatcgg aacgtaactc
     1381 atgccaatct ttcatatgat gcgaccgcct ccgtgctaaa tattcaatac taccttgaag
     1441 ggcaccagct tctgatgctc tttattccgt tttctgtaga tgcggaaccg gccgttgtcg
     1501 tcggtcgtgc tgtcaaacct gccatgaatg cacagattca ggacattctt cgatggagtg
     1561 gtgcgtccgt tctatcaaga cgcggcggag ataagtcgga gcagtttcct ccacacacta
     1621 cttcggcctc gccgccaaac tgagagggag gggcattcaa gttgaaaaga aagtacggct
     1681 tgaaaaatgg gaaaccccgt agctgtttca gccataccac cctgctcaat aaataattcc
     1741 tcccaccacc cagtgccggt catccgggat atcgatcagc aatcttgtga gattcatgct
     1801 ggccagctgc gaaccgatgt catcctccgt gaagacagcc cgtccaaaat cgtgatcaac
     1861 gcctgagaag aggttccgcg tctgcaattg atctttgacg gcactcgttg taaggtaacg
     1921 gccagcagga taaatgatca tgacgagcgt acacgaacaa gcgttcctcg aaaagcttcg
     1981 ccatcttccc cctgaacaag tggtagaagt agagcatttt gtcgacttcc tagtccagcg
     2041 cggagccgaa gaacatcgat tgactcaggc tgccagccaa gtcgcggtac ctgccttcgc
     2101 ccgcatttgg gataatcccg acgacgccga ctatgaccgg ctatgagttc ggggagatcg
     2161 ttctcgtccc gtttcccttc accgatcaat ccgccacaaa gcaccgtcca gccgtagtca
     2221 tcagcagtac cgcctatcat cgggcgcgcc ccgacctgat catcatggca gtcacgagtc
     2281 agcagccaac ctcggacgct ctcggggaag tggtggtcga agactggcgg gcagcgggac
     2341 tgctcaagcc atctgtgctc aaaccagtcc tgacgacgat tgatccgacg ctaattctga
     2401 agaagctcgg acgacttacc acgtcagatc aagtgcacct gcgacaggca cttgcacaaa
     2461 ttatcggcta atctcttcgg caacgagacg caccatatga agatggacta acagtcctgg
     2521 tcctggttca ccgtccatct caataaatga tccctcccac cacccagtgc cggtcatctg
     2581 ggatgtcgat caataatcgc gtgaggttca tgcgggccag ttgggaaccg atctcatctt
     2641 ctgtaaaggc cgcgaggaga gaattataga aatcccgtcg aagaatgtcc gattcgttcg
     2701 cagcgtactg gtcgacaata gcttgggccg cttccggcga ctcagagcgg agtagatcca
     2761 tcaccagcac cggcgctcca ggcttcacca actgacgaag tttctgccag aactgcaacg
     2821 gattcggcag atgatgcagc aggctattgg aaatcacggc atcaacgatt ctcgcgcccg
     2881 cgacttcttc atatcgttcg cagcgcagtg taatgcgatc ggttagatcc gcctgtttca
     2941 ctgcttgttc gcctaattga atcatcgggg ctgaagcatc gatgccgatg acctggcaag
     3001 ccggatagag cttggcgaat cggatcggaa tatcagcggg accgcagccc aggtctaata
     3061 ccatcccctg cgcaaactcc gggaagtatt ctttgaatcg attgacgaat ccctgattct
     3121 ctttcgcgaa gtcagcacgc gcataggctt ctgcttgctt ccgatcgtcc atcaactctg
     3181 gttcaagcac gcgatccatg gtccatacct taaaaggctg aggttaaggc tgaagctgaa
     3241 gtgagtgaga gcgaaaatcc ttatctcgac cccaacctca atctaaacct tttctatggt
     3301 cactgcgtct ccaggccgca ccacaccacc ttgcagcact ttcgcataga cacgactcca
     3361 gcctggattt aacttctgcg aaatgcgcga gaagtcctcg tcccgaaacc agctcttatt
     3421 gtggatgcag ggagttgtat agctcgtgac ttcgagttgg atctcagggc cgatcgtcaa
     3481 tcgaacacca ggtctcatca gctcccattc caatccagac aaggtcaagt tttcgcccga
     3541 cgacccgaca ttgatgggat ggccttcatc ctgcaaccgt tcgataagct cgagcgaaaa
     3601 tagacagact gcgcggtcag gtcctccgtg gaatttgagg ttccgctgtc gatccccatc
     3661 caagccccgt tcgctcacct tagcctccca aaccggcaac tttggaacgc ctccatcgga
     3721 gacattgatc tgatgcacat gtggataagg cggcccggtt gacatcgtca agcctctcca
     3781 ttgcatgatt ccggcctaaa aaatttcatc gccacccacc cttcatcctc acaccgttcg
     3841 gagcagacca atccgagatt ggaaaaccgt tcgacgattt cgtcttcctg ctcgaccaga
     3901 atgccggaga ccatgattct cgcccttcgc aatgcagaac atgccagatc gtcggcgaga
     3961 tccaagatgg tctgccggtc gagattggcg agcacaagat cggcgatcct tcgcttctcc
     4021 tcgggcag
//
LOCUS       DATNDD010000103         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2097677, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000103 DATNDD010000000
VERSION     DATNDD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097677"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="yidC"
                     /locus_tag="VN647_02440"
     CDS             <1..605
                     /gene="yidC"
                     /locus_tag="VN647_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWG95918.1"
                     /translation="HNYGLTIIILTVMIKLLFVPLQYKSYTSMKQMQGIQPKVAALQE
                     KFKEDRERLNKELIKLYRDHKVNPVGGCLPMVLQMPVFVSLFNILYMTIDLRQAPLGL
                     WITDLSVQDPYYILPIIMGISMVVMQKIQPTTMDPTQAKVMLMLPALMTFLFINFPAG
                     LVLYWLTNNVLTIVQQFVTDRFFFRIPAIAPATDEQVGKK"
     gene            658..2109
                     /gene="mnmE"
                     /locus_tag="VN647_02445"
     CDS             658..2109
                     /gene="mnmE"
                     /locus_tag="VN647_02445"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HWG95919.1"
                     /translation="MSAVGTPEDTICAIATPVGEGGIGIVRISGQHAVGIGSRVARLR
                     SRQSFHTLASHKLHLADILFPSVPSIDNLPGSETGQSTDQMIDEGLVVYMKAPRSFTA
                     EDVVEIHCHGSGLVLQRVCEACVAAGARLAQAGEFTKRAFLNGRLDLSQAEAVLDTIR
                     AKSELGLKLAQRQLRGELAQQVHRLRAGLVGLLAHLEAGIDFAEEDIAFVGRDEMIVS
                     LQETLSHVQRMLTTAETGRVLRDGVRVVIAGEPNVGKSSLLNSLLRENRAIVTDIPGT
                     TRDIIEESVVWLGLRVTLTDTAGMRDTTDLVEREGIRRSKEAQDQADIVLHVLDGSQL
                     TTSDLGNVCLPSVAHEHLLVVNKIDLVDAASVQSLSQALRARTPCKILPISVRTGQGL
                     EALKVAIRAQFVKPSFEPSDGVVVTHVRHRVALERAETSLHESLVSIERGIEPEFVAV
                     DLRGAADALGEIVGAITSDEVLDRIFSEFCIGK"
     gene            2142..>2269
                     /locus_tag="VN647_02450"
     CDS             2142..>2269
                     /locus_tag="VN647_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWG95920.1"
                     /translation="MVGGGHAGCEAALAAARMGAKTLLLTMELGRIAQMSCNPAIGG"
BASE COUNT          495 a          591 c          636 g          547 t
ORIGIN      
        1 cgcataatta tggcctgacg atcattatct taaccgttat gatcaaactg ctatttgtcc
       61 ccctgcaata taagagttac acgtcgatga agcagatgca ggggatccag ccaaaagtgg
      121 cggctctcca ggaaaaattc aaagaagatc gggaacgctt gaacaaggag ctcatcaagc
      181 tctaccggga tcacaaggtg aaccccgtcg gcggctgtct ccctatggta ttgcaaatgc
      241 cggtgttcgt atcactgttc aatattctgt atatgacgat cgatttgcgc caggcaccgt
      301 tggggttgtg gattaccgat ctctcggttc aggatcctta ctacattcta cctatcatca
      361 tgggaatcag catggtggtc atgcaaaaga tccaaccgac cactatggac ccgactcagg
      421 cgaaagtcat gctgatgctt ccggcactca tgacgttcct attcatcaat tttcctgccg
      481 gcctggtgct gtattggttg accaataatg tgctcaccat cgtacaacag ttcgtcaccg
      541 atcgcttttt ctttcggatc cccgcaatag caccggctac ggacgaacag gttggcaaga
      601 aatgatgctc ggtcggtttc gctcgatgtg agttcgtccc tccgaatgtg tgtgaacatg
      661 tcggccgttg gaacacccga agataccatt tgtgcgattg ccactcctgt cggtgaggga
      721 gggatcggta tcgttcggat aagcgggcag catgccgtag gcatagggag tcgcgttgcc
      781 cggctgcgtt ccaggcaatc gttccatacg cttgcttctc acaaattgca cctggccgat
      841 attctctttc cgtctgttcc ttcaatcgat aacctgcccg gctccgagac aggtcaatcg
      901 accgaccaga tgatcgacga aggtctggtg gtctatatga aggcacctcg atcttttacc
      961 gcggaggacg tggttgagat tcattgccac ggaagtgggt tggttctaca acgagtctgt
     1021 gaggcctgcg tcgctgccgg agctcgtctc gcacaggcag gagagtttac caaacgggcc
     1081 ttcttgaacg gccgcctgga tttgtctcag gctgaggctg tgttggacac catcagggcc
     1141 aagtccgaac tcggccttaa attggcacaa cgtcagctcc gcggcgaact cgcacagcag
     1201 gtccaccgac tgcgcgccgg tctcgtaggc ctgttagctc acttggaagc aggaatcgat
     1261 ttcgccgagg aagatattgc gttcgttgga cgtgatgaga tgatcgtctc cttgcaggag
     1321 acgctgtccc atgtccagcg gatgttgacg actgctgaga ccggacgcgt actgcgcgac
     1381 ggcgtccggg tggtgatcgc cggagagccc aatgtcggta agtcaagttt actgaatagt
     1441 ttactccgtg agaaccgagc tattgtgacc gacatcccgg gcacgactcg cgacattatc
     1501 gaagaatcgg tcgtttggct tggtctgcga gtgaccttga ccgacaccgc tgggatgcgt
     1561 gacacaacgg acctcgtcga acgagaaggt atccgccggt ccaaggaagc tcaggaccag
     1621 gcagatatcg tcctgcatgt cttggatggc tctcaactga caacgagcga cctagggaat
     1681 gtctgtttgc cttcagtcgc tcatgaacat ttgcttgtcg tcaataaaat cgatttagtc
     1741 gacgctgcat cggtacaatc gctctctcaa gcacttcgag ctcgtacacc gtgcaagatt
     1801 cttcccatct cggttcgcac gggtcaggga ttggaggcat tgaaggtcgc tattcgagca
     1861 cagtttgtga agccgtcctt tgaacccagc gatggtgtcg ttgtcactca tgtgcgacat
     1921 cgcgtggcgc tcgaacgggc cgagacatca ctccatgagt cactggtctc gatcgaacga
     1981 ggcatagagc ccgagtttgt ggcggtcgat ctccgaggtg cagccgatgc gctgggcgaa
     2041 attgtcgggg caattacatc cgatgaagtg ttggaccgca ttttttccga gttttgtatc
     2101 ggtaaataga ggaaccggtg gccacagcat ttgacgtcat cgtggtgggg ggcggtcatg
     2161 ccggctgcga agccgcactt gccgccgcac gaatgggcgc gaaaactttg cttttgacaa
     2221 tggaactggg acgaatcgca cagatgtcgt gcaatccggc aatcggtgg
//
LOCUS       DATNDD010000104         6454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_594861, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000104 DATNDD010000000
VERSION     DATNDD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594861"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VN647_02455"
     CDS             <1..557
                     /locus_tag="VN647_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG95921.1"
                     /translation="LNIETAQRTRSDQRVEAKEADNRMIQQINQLIHEVRGMIRELES
                     GSVQEFDLSSELFALCTTYEQTGRLHIRLDLQQSAIDVLTNEEEREILNIAREALSNC
                     ARHADATRAVVSIRMREAKIRVSIHDNGIGFSTAVGQPRGYGLANMEARAKRLGGALR
                     VQSKTGEGTQVIAEFSLEPLLTSI"
     gene            554..1234
                     /locus_tag="VN647_02460"
     CDS             554..1234
                     /locus_tag="VN647_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG95922.1"
                     /translation="MKQSSTSIVLIDDHEMVRRGLRALLHLEPDMAIVGEAATVADGI
                     AAVERLAPQMVLLDVKLPDASVTEACRRLLAVAPKLRILVLTSYAEDSTVMAAVQNGA
                     HGYVLKDVRMDDLIRAIRTVAGGQGYLDPRVTQQALHWIRSSSHLGAASLGTSRLSPQ
                     ERLILPLLAEGKTNKEIAVQLRLSDKTVKNYLANIFDKLHVKRRTEAVAWFMKETHMS
                     GAARNQKL"
     gene            1443..3341
                     /locus_tag="VN647_02465"
     CDS             1443..3341
                     /locus_tag="VN647_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG95923.1"
                     /translation="MSIELVDLRRQVLELQQALARAERHRVPEHRATLGAAQVGMWEW
                     EGRTDLKRAEEAKTKSETRLRSFLDALDDLAFEFDENGRYLNVWTRNEDKLLLPKQDL
                     IGKRLIDIFGPETGSHYLETIRRVIATGKPATIDYTVSLGGELRYFSGILTLIPGSGA
                     DSATVGCIVRDISDRKRSYSLLQAAINSVADGLLVIDRQGKVTSVNQRFLHLWNIPQA
                     PADDRDDEELLAFVVDQLREPEAFLRKVRELYENPKQESFDVLDFKDGRVFERYSRPQ
                     ILDGEIVGRVWSFRDVTEHRRAEEALRAGERTLQRFVAEAPVGLCILDENWRAISANK
                     AFCELTGYEEHEIIGSTYALYTHPEDLSANIVLTDEFFQGIRSGYTYEKRYIRKSGEI
                     IWVSVKATRVELSGHAGPLLLAAVQDVTERKLAMEEREHLSRDLHDNILQALYAVGMQ
                     LEAGKLSMGKSPRRSKIHMTQAVDQLNNLMLDVRRFIALLAHRTTTEPDFGRALQQLI
                     ASMPGAGQSAPELDITNPVLSFITPKLGEQLLNIVREALSNSSRHARASRRWVRLSLE
                     DNTIHLIVGDNGVGFSSKRKRRGGNGLGNMAARAKQISATFTLESAPGKGTTVLVDVP
                     LKKGTVYE"
     gene            3334..4068
                     /locus_tag="VN647_02470"
     CDS             3334..4068
                     /locus_tag="VN647_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG95924.1"
                     /translation="MNKSKPIRLVIVDDHEVVRIGLCAVLNLTPGMKVVGQGGRVKEA
                     KKLCHHLKPDLVLLDIRLPDGSGVDAAREILAKCPDTRVLFLTSFADDHTVLEAILSG
                     AQGYILKDIASNALIRAIRTVASGQALIDPRLTKHTLEWLSTMYSEPGQSKRSLLSPQ
                     EQRLLPLVAKGLTNKEIAHDLRLSEKTVKNYLANIYSKLNIGRRSQIAAFYAGSFKGS
                     GALYPSRLSESVKADRERTTPHLNSY"
     gene            4285..4860
                     /locus_tag="VN647_02475"
     CDS             4285..4860
                     /locus_tag="VN647_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HWG95925.1"
                     /translation="MSDSHSPESREAHRSLKPSHSASNQVEAFFESIVFASRWIQAPL
                     YAGLIVAELLYAYKFLIELWEMVIHIGKMEETVFMLGVLGLIDVTMVANLLTMVVIGG
                     YATFVSKLDLEHHPDRPDWLTHVDPGTIKIKLAASLIGISSIHLLKGFVDVANENPEH
                     LKWKIFIHMTFLASAILLAWTDKIMQKDKKH"
     gene            complement(4952..5725)
                     /locus_tag="VN647_02480"
     CDS             complement(4952..5725)
                     /locus_tag="VN647_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG95926.1"
                     /translation="MRQKDHGHGREQQNQKWIVPDPSGITTSRSRPIRVFIADGHEVV
                     RAGVCALLEGEQDVEVVGEADNAEGVLTESQRTKPDVLLLKSGLSGGSDSDIYKTLFN
                     VLPAVRIISLMRDGDAEAFRNAVEGGAQGYLWEKTGRIELIRAIRTVAKGNSYLGPET
                     AAQTFRLLRARQDSVCSPSALHTLSRQEQRVIALIAEGNTNKQIATKLVLSEKTVKNY
                     IVSIFAKLKIERRTQAAALYMKVQQHQNSIREEISARVE"
     gene            complement(5860..>6454)
                     /locus_tag="VN647_02485"
     CDS             complement(5860..>6454)
                     /locus_tag="VN647_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG95927.1"
                     /translation="RQAIRGVIESRFPSSVVQEASTGEEAVRIVRQERVDVAILDIGL
                     PDFSGLTVLRRVKQVRPSLLCLVLTMHDNPQYVRLAMAHGASGYLTKGATSGELYDAI
                     RTILSGRQVVMELFRETLDRRSMRRDVIWPHESLSVRELEVLSLLAKGRTVSQVAKRL
                     KLSVKTVSTYRTRLLEKLHLGTTADLIRYAVDHQLVR"
BASE COUNT         1473 a         1811 c         1739 g         1431 t
ORIGIN      
        1 gtttgaacat tgaaacggct cagcggactc ggagcgatca aagggtggaa gcaaaagagg
       61 ccgacaaccg gatgatccag cagatcaatc agttgattca tgaggtccgt gggatgattc
      121 gagaattgga atcaggaagt gtccaagaat ttgacttgtc gtcggagctc ttcgccttgt
      181 gcacgaccta tgagcaaacg gggcggctac acataaggct ggatctccaa cagagcgcca
      241 ttgatgttct aacgaacgag gaagagcggg agatcctgaa tatcgcgcgg gaggctctca
      301 gcaactgcgc gcgccacgcc gacgccaccc gggctgtcgt ctcaatccgc atgcgagaag
      361 ccaagattcg ggtgagcatt catgataatg gcatcggatt ttcgacggcg gtcgggcagc
      421 cacgaggata cgggcttgcc aatatggaag cccgagccaa acgactcgga ggggcgttga
      481 gggttcagtc caagaccgga gaaggcacgc aagtcatcgc ggagttttcc ttggagcctc
      541 ttttgacgtc catatgaaac agtcctcgac cagtatcgtc cttattgacg accatgagat
      601 ggtccgtaga ggactccgcg cgctgcttca cctcgaaccg gatatggcga tcgtcgggga
      661 ggccgcaacc gtggcggacg gtatagcagc cgttgagcga ctcgcccctc agatggtatt
      721 actcgatgtg aagcttcccg acgcctcggt caccgaggcc tgtcgacgcc ttctggccgt
      781 tgccccgaag cttcggattc tcgtcctcac cagttatgcc gaagattcca ccgtgatggc
      841 cgcggtccag aacggggcac acggatatgt actcaaggat gtccgaatgg atgacctgat
      901 tcgtgccatt cgcaccgtcg ccggcggcca gggctatctg gaccctcgag tcactcagca
      961 agccctgcat tggattcggt ccagttcaca ccttggagcg gcttcccttg gaacttcccg
     1021 actgtcccca caagagcgct tgatcctccc gcttctggct gaaggaaaaa cgaataagga
     1081 gatcgccgtc cagcttcgct tgagcgacaa aaccgtcaaa aactacctgg caaatatctt
     1141 tgataaactt catgtcaagc gtcgtacgga ggccgttgcc tggttcatga aggaaaccca
     1201 tatgtccggt gctgcgcgaa atcaaaaatt atagcgatcc tgtactctgc tcgcgcccgt
     1261 ggcgctcctc cttcataccc ccctgacccc cgacgattct ctggcattgg tagacccgca
     1321 ggttctatac tacttattca agatagacct acctgtgaag gaagcgggct ggccttagag
     1381 cttcatccgg catgacatcg ggactcttct ttatgagcgc gccgctggag accgacagat
     1441 caatgtccat cgagcttgtc gatctgcgcc gccaggtttt ggaattgcaa caggcgttgg
     1501 ctcgtgcaga acgccaccgt gtccctgaac acagagcgac gctcggggcc gcccaagtgg
     1561 gaatgtggga atgggaaggc cgcacggacc tgaagcgggc cgaggaagcc aagaccaaga
     1621 gcgaaacccg gctccgctca ttcctggacg ctctggacga cctcgccttc gaattcgacg
     1681 agaacggacg atatctcaac gtgtggaccc gcaacgaaga caaactcctt cttcccaaac
     1741 aggaccttat cggcaagcga ttgatcgaca tcttcggacc ggagaccggt tctcactatc
     1801 ttgaaacgat tcgtcgtgtc attgcgacag gcaaaccggc gaccattgac tatactgtgt
     1861 cgctgggtgg ggagttacgg tatttttctg ggattttgac gcttattccg ggaagcggag
     1921 cggatagcgc cacggtcggg tgcatcgtac gcgacatctc cgaccgcaaa cgttcttact
     1981 cactccttca agccgccatc aactcagtcg ccgacggcct gctcgtcata gaccgacagg
     2041 gtaaggtgac cagtgtcaac cagcggttcc tgcacctatg gaacattcca caagcaccgg
     2101 cggacgaccg ggatgatgaa gagctgctgg catttgtcgt cgaccaactg cgggaaccgg
     2161 aggcattttt gaggaaggtt cgagagctgt acgagaatcc gaaacaagag agcttcgatg
     2221 tccttgactt caaggacgga cgggtcttcg agcgctattc tcggccacag attctcgatg
     2281 gtgaaatcgt cgggcgcgtc tggagtttcc gagatgtcac cgaacaccga cgcgctgagg
     2341 aagccttacg tgcaggcgag cgtacgcttc agcgcttcgt ggctgaagcc ccggtcgggt
     2401 tatgtatcct cgatgaaaac tggcgcgcga tcagcgccaa caaagccttt tgtgagctga
     2461 cgggatatga agaacatgaa attatcggca gcacctatgc cctctataca cacccggaag
     2521 atctttccgc caatatcgtg ttgacggacg agtttttcca ggggattcga tcgggatata
     2581 cctacgaaaa gcgatacatt cgtaagtccg gtgaaattat ttgggtttca gtcaaagcga
     2641 ctcgtgttga gttgtcaggc catgccgggc cgctcctcct tgccgcggtt caggacgtta
     2701 ccgagcgcaa actggcgatg gaggaacgcg agcacttgag ccgagacttg cacgacaata
     2761 ttttgcaagc cctctatgcg gtcggcatgc agttggaagc cggcaagttg tcgatgggga
     2821 aatcccctcg gcggtccaaa atccatatga ctcaagccgt tgaccagctg aataatctga
     2881 tgctcgatgt tcgacggttc attgccttgc tggcgcaccg caccacaaca gagccggact
     2941 tcggacgggc cctgcagcag ctcattgcat cgatgcccgg cgccggccaa tccgcacctg
     3001 aactggacat cacaaacccg gtcctgtcct tcatcacccc caagctggga gaacagttac
     3061 tcaacatcgt acgagaagct ctcagcaata gctctcgtca tgcccgcgcg tctcgtcggt
     3121 gggttcgact cagtctggag gataacacga tccacttgat cgtcggcgac aatggggtcg
     3181 gattttcctc caagcgcaaa cgtcgaggag gtaatggact aggcaatatg gccgcccggg
     3241 ccaagcaaat ctccgccacc tttacgctgg agagcgcgcc ggggaaggga accaccgtcc
     3301 tagtcgatgt tccgctgaag aagggaactg tgtatgaata agtccaaacc gattcgcctc
     3361 gtcatcgttg acgatcatga agtcgtacgt atcggactct gtgcggtcct caatttgaca
     3421 cccggcatga aagttgtggg acaaggtgga cgggtgaaag aggcgaagaa actctgtcat
     3481 catcttaaac cggacctcgt gttactcgac atccggttgc cggacggcag cggagtggat
     3541 gccgcgcgcg agattcttgc caagtgtccc gacactcgcg tgttgttctt aacgagcttc
     3601 gccgacgacc ataccgtact cgaagctatt ctttccggcg ctcaaggcta catcctgaaa
     3661 gacattgcgt ccaatgccct gatccgcgcc attcggaccg tagcctcagg tcaagctctc
     3721 atcgatcctc gcttgacgaa acatacactg gaatggctga gtactatgta tagtgagccg
     3781 gggcaatcca aacgctctct tctttctcca caagagcaac gtcttttgcc gttggtggcc
     3841 aagggcctca cgaataaaga gatcgcccat gatctgcggt taagcgaaaa gaccgtcaag
     3901 aactatctcg cgaacatcta ttcaaaactg aatatcgggc gccgatccca gattgccgcc
     3961 ttctatgccg ggagctttaa gggttcaggt gcactgtacc catcgaggct cagtgaatcg
     4021 gtcaaagctg accgcgaaag gacgactcct catcttaact cctattgatg ctcgtctgtt
     4081 cccgcgagga agggcgccac cccatcgcgg atcaactcct catttgtcca gatatcaaac
     4141 tttttccata caccactccg gtggcctacc gagagggtaa agcattgaat tgacagcttt
     4201 gaattcgctg actaggatac gcctcgcccc tcgattcgtc actgggggca tgtcatctca
     4261 tcgttggact gcgaggaggt tttgatgtcc gattctcatt cgcccgaatc ccgagaggct
     4321 cacagatcac tgaagccctc tcactcggcc agcaatcaag tcgaggcatt tttcgaatcc
     4381 attgtctttg ccagtcgatg gattcaagct ccgctctatg ccggactcat cgtggcggaa
     4441 ctgctctacg catataaatt tctcatcgag ctctgggaaa tggtgatcca tatcggcaag
     4501 atggaagaaa cggtattcat gctgggagtc ttggggttga tcgatgtcac aatggtcgcc
     4561 aatctactga ccatggtggt catcggcggg tatgccacgt tcgtgagcaa gctggatttg
     4621 gagcatcatc cggaccggcc ggactggctg acccacgtcg atcccggcac catcaagatc
     4681 aagctggcgg cttctctgat cggcatctcc agtattcatt tattgaaagg attcgtcgac
     4741 gtcgccaatg aaaacccgga gcatctgaag tggaaaatct tcatccacat gaccttctta
     4801 gcttctgcca tcttgctggc atggaccgac aagatcatgc agaaggacaa gaaacattga
     4861 gcaatggagg gaacctcctc gaaatccggc gcgacctcca gaggcccgaa tccgatggtc
     4921 attcttcacg gcaagaatcg cgggcataaa gctattcgac tctcgcagaa atctcttcgc
     4981 gtatcgagtt ctgatgttgc tgcaccttca tgtaaagggc agccgcttgg gtgcggcgtt
     5041 cgatttttaa ctttgcgaaa atgctgacaa tgtaattttt taccgtcttt tccgacagca
     5101 ccagcttcgt ggcgatctgt ttgttggtgt tcccttcggc gatgagtgca atcactctct
     5161 gttcctgccg tgatagcgta tgcaacgcgg aaggagagca aaccgaatcc tgcctggctc
     5221 tcagcaagcg aaaggtctga gcggcggtct ccgggccgag gtatgaattt cctttcgcga
     5281 cggtacgaat cgctcgaatc agctctatcc ggccggtctt ctcccataga tacccttgtg
     5341 cgcccccctc gactgcgttg cggaacgcct cagcgtcacc atctctcatc aaactgatga
     5401 tccgaactgc tggaaggacg ttaaagaggg ttttgtagat gtcggagtcc gaaccgcctg
     5461 aaagcccgga cttcagtaaa agcacatccg gcttggttcg ttgcgattcg gtgagtacgc
     5521 cctccgcatt gtcggcctca ccgaccactt cgacgtcttg ctctccctcc aacaacgcac
     5581 acaccccggc tctgacgact tcgtggccat cagcgataaa tacgcgaatc ggtcgagagc
     5641 gtgaagtcgt aatgcctgag gggtctggga caatccactt ttggttttgt tgctcacggc
     5701 catggccatg atccttctgt cgcatccaaa caaccccggt gagtagagtc tccgcactga
     5761 ctcgattctt ctcagaagct accaggaacg ccgagcctag ttatcaggtc gaacctcaac
     5821 ccggtatcgg tttttgtcct acaggggagt catcggctct tagcgcacaa gctgatgatc
     5881 gacggcataa cggataagat cggcagtcgt tccaagatgc agcttttcaa gcaaccgggt
     5941 tcgatacgtg ctcacggtct tgacactcag cttcaatcgc ttcgcgacct gtgagaccgt
     6001 gcggcctttc gctaagagtg agaggacttc caactcgcgc acggaaaggg attcgtgcgg
     6061 ccatatcaca tcacgacgca ttgagcggcg gtcgagtgtt tcgcggaaca attccatcac
     6121 tacctgacgt ccggacagaa tggtccggat ggcatcgtac agttcccctg acgtcgctcc
     6181 tttggtgaga tagccggagg ccccgtgagc catggcaagg cgcacatatt ggggattgtc
     6241 atgcatggtc agcaccagac acaggaggga aggacgcact tgtttgacac gcctcaacac
     6301 ggttaagccg ctgaaatccg ggagaccgat gtccagaatc gccacatcca cgcgttcttg
     6361 ccgtacgata cggacggctt cctctccggt agacgcttcc tgtaccaccg atgaaggaaa
     6421 acggctctcg atgactcccc tgatagcttg ccga
//
LOCUS       DATNDD010000105         4540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3531952, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000105 DATNDD010000000
VERSION     DATNDD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531952"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VN647_02490"
     CDS             <1..229
                     /locus_tag="VN647_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95928.1"
                     /translation="FKTPGLRDPDHSAPFMHNGRSDTLDDVINSYINRSNEARAGALR
                     NGDSRLQGIALKTNDIASLVAFLKSLNEDYQ"
     gene            complement(656..1126)
                     /gene="bfr"
                     /locus_tag="VN647_02495"
     CDS             complement(656..1126)
                     /gene="bfr"
                     /locus_tag="VN647_02495"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWG95929.1"
                     /translation="MKAKEGVLEHLNQVLKAELTAVHQYLLHAAMCKNWGYDRLHDYY
                     SHVANEEVQHSAGLIDHILYLNGAPEVGQLDTVAQGQDVGVLFRVDLDFEREDVELLR
                     KAIAHCATVGDFTTRHLLEHMIEDSEEHVDWFETRLRTISQVGLDRFLSEQIKK"
     gene            complement(1135..1611)
                     /gene="bfr"
                     /locus_tag="VN647_02500"
     CDS             complement(1135..1611)
                     /gene="bfr"
                     /locus_tag="VN647_02500"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWG95930.1"
                     /translation="MKAKEGVINILNKILTADLTAINQYFVHAKMCSNWGYDRLHHKV
                     RERSIDEMKDADELIGHILYLEGVPNMQRMNAVQVGETVPEQLKLDLKAEQEMLALLN
                     EGVVHCTKAADFTTRHMLEDMAKDVDGHIDWIETQLETIKQIGVEIYLAEQIKKES"
     gene            1883..2470
                     /locus_tag="VN647_02505"
     CDS             1883..2470
                     /locus_tag="VN647_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95931.1"
                     /translation="MIKQVRRFAPPLFLLALTAIFSPSLAAAHGNVSIEEDICVRRIG
                     GSMVHFSAYQPQIEPKAQYCTDIPDVGDTFLVVDLVDPSLRNLPIGVKIIRGVNENEQ
                     DEAKTVAYWKPASHPDGIVRGEAKLDKGLYKLIITAEGLSPSSYLLRVQQIDYSKLGR
                     AALGPLALLLVLGIVGYELSKSSRVRGWFASRRRS"
     gene            2632..3120
                     /locus_tag="VN647_02510"
     CDS             2632..3120
                     /locus_tag="VN647_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95932.1"
                     /translation="MAEDMCKLTVGPYMMHFSGYQPENSQQKQFCEDIPAVGQTIVVL
                     DYIEQELRSLPAEVRIIKDTGSEDNLETITVFHLPAKVYPNGSIDFAYTFDKPGKFVG
                     MVTVGDKQQHISRFPFSVGEPKVFSKFLNIYMVPVAAVVIVGAIVFFMRDRGKPSQIA
                     AS"
     gene            3183..3770
                     /locus_tag="VN647_02515"
     CDS             3183..3770
                     /locus_tag="VN647_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95933.1"
                     /translation="MSCHLLIRAWVCCLFLLVLAPFSALAQHGHELATDTCVLHIGPY
                     KMYFNSYQPDTYYDRQFCQELPGTGNTVLVLDYVERELRSLPVEVRIIRDTGSEDNLE
                     ALTITHIPAKVYPTGSIDVKYNFDKPGKFVGLVSIGDKREHVSRFPFSVGETGAVSHL
                     THYIMVIVPVLVGIAVAVFFSFRDRRKPSGIPVSS"
     gene            3860..4261
                     /locus_tag="VN647_02520"
     CDS             3860..4261
                     /locus_tag="VN647_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWG95934.1"
                     /translation="MIGSPGRHMFRPVILIVAFVLGVPVTSAFAHSMLVKAEPPRRAI
                     LTKAPIQVRLWFNEKIEGDYASLVVFDAKKQPITDLKPTLSSDDPKSIVLPLPELAPG
                     KYSVKFRVLSVDGHVVESSFDFTVKGEAQKK"
     gene            4258..>4540
                     /locus_tag="VN647_02525"
     CDS             4258..>4540
                     /locus_tag="VN647_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95935.1"
                     /translation="MIEAAGALFRCLQLASSMLLIGGCVFLAIAELKASASGHTWPTR
                     LKQTFPWLAVTVLLGLFGLLATTTAQATGVPENVRNPQAWLDFLQKTRIG"
BASE COUNT         1038 a         1263 c         1147 g         1092 t
ORIGIN      
        1 attcaaaacg ccgggccttc gcgatcctga ccattccgct cctttcatgc acaacggtcg
       61 atcggatacg ttggacgacg tgatcaattc ctatatcaac agatcaaacg aagctcgcgc
      121 aggtgcgctg cgcaatgggg atagccggct tcagggcatc gcgctcaaga ccaacgacat
      181 cgcatccctg gtggcatttc ttaagtcgct gaacgaggat tatcaataat caacccggct
      241 ggtatcgaga taacaactgt tttcccatgc aacaatcccg ctccgattct gagacactga
      301 tctgcaaccg ttgcaacaga ccgacgggag accagagttc ccgcctctgg tgcaaaaact
      361 gctggctatt caatcgtcag caagggcaag cacgcgggct cgcgaaatcg ctccgaacgc
      421 gacggctcaa agcatggctg aaaacttttc cctaatccat agctttttca aacgtatgac
      481 ggtgtggaca atgaaatgtc agcatgcgtt cagaccagtg acatggtccc cgcaaccctg
      541 cctcagcggc atttcgccat gcgacagcgt tagaagaggt cggagtctcc cggctcacct
      601 cccatgagcc ggagaagaca tccgaccgta tagccgaaac aggcaggacc agcacctact
      661 tcttgatctg ctccgataga aaccgatcga gaccaacctg actgatggtc cgaagccgag
      721 tctcgaacca gtcgacatgc tcttcagaat cctcgatcat atgttccaat agatgacgcg
      781 tcgtgaaatc accgacggta gcgcagtgag cgatcgcctt tctgagcaat tcgacatctt
      841 cacgctcgaa atccagatcg actcgaaaca acactcctac atcctgcccc tgtgctaccg
      901 tatcaagctg tcccacctca ggcgcaccgt ttaagtacaa gatatgatcg atgagtccgg
      961 cggagtgctg cacttcttcg ttcgccacat ggctgtagta gtcatggagt cggtcataac
     1021 cccaattctt gcacatcgcc gcgtgtaaca gatactgatg cacggccgtc aactcggctt
     1081 ttaacacttg attgagatgc tcaagcacgc cttctttggc tttcatggtt tccctcagga
     1141 ttctttcttg atctgttctg ccagatagat ttccacgccg atttgcttga tggtttccaa
     1201 ttgggtttcg atccaatcaa tatgtccgtc gacatccttc gccatatctt cgagcatgtg
     1261 acgggtcgta aaatcggcgg ccttcgtaca gtgcacgacc ccttcgttca acaacgccaa
     1321 catctcctgc tcagccttca agtccaattt aagctgttcg ggcaccgtct cgcccacctg
     1381 gaccgcgttc attcgctgca tattgggcac accctccagg tagagaatat ggccgatgag
     1441 ttcgtcggca tctttcatct catcgatact tcgttcgcgc actttatgat ggagccgatc
     1501 atagccccaa ttgctgcaca tcttggcatg gacaaaatat tggttgatgg ccgtcaagtc
     1561 ggccgtcagg atcttattga gaatgttgat gaccccttct ttggctttca tgatgagtgc
     1621 ctcctttcat accgagctca cggtttttct tattatcatc ctgctcgcac gagtcgtcaa
     1681 caggtaaata caagtattac gataacgcct ctcaatttcg atgaaatgaa ttctcagtat
     1741 tagccgatcg cgtcggcctc acggcgacgt gcatctgatg ccggccactg cggaggggtc
     1801 gagtacttga aagaacagtc tgcggggtgg tagattccca caaatctaca ccgtccagta
     1861 cattgacacc gcagatgaag tcatgattaa gcaggtaaga cgttttgccc cgcccctttt
     1921 cctactggct ctcacggcga tattcagccc cagcctcgcc gccgcgcacg gaaacgtgtc
     1981 gattgaagaa gacatttgcg tacgaagaat cggcgggagc atggtgcatt tcagcgcgta
     2041 ccagccgcaa attgagccga aggctcaata ctgcacggat atcccggatg tgggtgacac
     2101 cttcctcgta gtggacttgg tcgacccaag cctgcgcaac ctgccgatcg gagtcaaaat
     2161 cattcgaggg gtcaatgaaa atgagcaaga cgaggccaag actgtcgcgt actggaaacc
     2221 ggcttctcac cctgacggca tagtgagggg agaggcgaag ttggataagg gcctctataa
     2281 attgatcata acggcagaag ggctcagccc ttcctcctat ttactgcgag tgcagcagat
     2341 cgactattca aaactcgggc gggcagcgct aggacccttg gccctgctgc tggtacttgg
     2401 aatcgtcgga tatgagctct cgaagtcgag ccgagtgcgg ggttggtttg cgtctcgccg
     2461 tcgttcgtga gcagccggcg cggcgtgtga cgatctgagc attcatttca ccacatcaaa
     2521 gagggatctt ccatgaggcc tttcgtagat ctgaaactaa cggttggttg tgcggtattt
     2581 gctatgggcg tcctcgtatc ctcacaggtc catgctcatg gcggactctc catggctgaa
     2641 gacatgtgca aactcacagt cggtccctac atgatgcatt tcagcggcta ccagcctgag
     2701 aactcgcagc aaaagcagtt ctgtgaagac atcccggcag tcggccaaac gatcgtcgta
     2761 ttggattaca tcgagcagga acttcgttcg cttcccgccg aagtccgcat catcaaagac
     2821 acgggttcag aggacaatct tgagactatt acggtgtttc acctcccggc caaggtgtat
     2881 ccgaatggtt ctatcgactt tgcctacacc tttgacaagc cggggaaatt tgtcggcatg
     2941 gtcaccgttg gtgacaaaca acagcatata tcccggttcc cattctcagt aggcgaaccg
     3001 aaggtctttt ctaaattctt aaatatctat atggttccag tagccgcggt agtcattgtc
     3061 ggtgcgattg tctttttcat gcgcgaccgc ggcaagccgt cgcaaatcgc tgcttcctag
     3121 gatagcgcga cgacgggacc gttacggatc atgagaagca gagaaagaag agagggttga
     3181 acatgtcctg tcacttgctg attcgagctt gggtgtgttg cttgttcctg ctggtcttag
     3241 ctccgttctc ggcgctggcc cagcatggac atgaattggc gaccgatacc tgcgtcctcc
     3301 acatcggccc atacaagatg tacttcaaca gttatcagcc tgacacgtat tacgacaggc
     3361 aattctgcca ggaacttccg gggacaggga acacggtcct ggtccttgac tatgtcgaac
     3421 gggagcttcg ttccctcccg gtcgaagtcc gtatcatcag agataccggc tcggaggaca
     3481 atctagaggc tctgacgatc acacacatcc cggctaaagt ctacccgacc ggctccatcg
     3541 acgtgaaata caactttgac aagcccggca agttcgtggg cctggtttca atcggtgaca
     3601 aacgagaaca tgtctcacgc tttccgtttt cagtcggaga aacaggagct gtttcgcacc
     3661 tgacacacta tatcatggtc attgtgcccg tcctggtagg cattgctgta gccgtgttct
     3721 tttcctttcg ggatcgtcgc aaaccttcgg gaatccctgt gtcatcataa gaggcgacgg
     3781 atgcccgctg gagcaccgaa tgagacagcc ggatgcgtca cacttccttg gataccataa
     3841 tcgagcacat ggaggatcaa tgatcggctc accaggcagg cacatgttca ggcctgtcat
     3901 cttgatcgtg gcgttcgtct tgggagtccc cgtcacctcg gccttcgcgc attcaatgtt
     3961 ggtcaaggcg gaaccaccac ggcgggccat tctcaccaag gcgccgattc aagtgcgctt
     4021 gtggttcaac gagaaaatcg agggagatta tgcctctctc gtagtcttcg atgccaagaa
     4081 acagccgatc accgacttga aacccaccct gtcctctgac gatccgaaat cgatcgtcct
     4141 gccgttgccg gagctggcgc ccggcaagta ttccgtcaag tttcgcgtcc tctcggtgga
     4201 cggacatgtc gttgagtcgt ctttcgactt caccgtaaag ggcgaagcac aaaagaaatg
     4261 atcgaggctg ctggtgcgct gtttcgctgt ctgcagctgg cctcctctat gctgctgatc
     4321 ggtggttgcg tttttttggc aatcgccgag ctgaaggctt ctgcttccgg acatacgtgg
     4381 ccgactcgtc tgaaacagac ctttccatgg ctggccgtaa cagtgttgct tggattattc
     4441 ggtctcctcg ccacaacgac ggcgcaagca accggagttc ctgaaaatgt tcggaatccg
     4501 caagcctggt tagacttctt acaaaagacc cgcatcgggc
//
LOCUS       DATNDD010000106         5070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4172361, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000106 DATNDD010000000
VERSION     DATNDD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4172361"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VN647_02530"
     CDS             <1..836
                     /locus_tag="VN647_02530"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWG95936.1"
                     /translation="LTSVPTWSLRPPSVAQMCRYVVLVAVAALSSYSAGSIEAEPLGE
                     TKRPFQVGERLTYEVSWLNLTAVIAVMEVARIDGRKDQAVAKLVGTAQSTPVITKFFP
                     VDNRVESELDLDTLTPEHMTFRRREGKKKEDIEYVFHQKEGVVTAVRGGTTESLPIPV
                     GTQDIISCLYYTRTRLPPNPGASLKMNVYHDKKNRPVEVRVEAIETIEGAWGRAETVR
                     VLVIMPFHGLFMNKGNIRVWVTNDERKTPVRMTAKVVLGSIVADLVDGFEESGTAEQK
                     Y"
     gene            886..1890
                     /gene="fbp"
                     /locus_tag="VN647_02535"
     CDS             886..1890
                     /gene="fbp"
                     /locus_tag="VN647_02535"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HWG95937.1"
                     /translation="MREFPLTLGRFIIQNQTSHQDATEEFSSLLTQIGLVGKLISQDL
                     RRAGLINILGTTGDTNVQGETVKKLDAIANDDFVKVFQHSGYVCALASEEMEKPISIP
                     GNWPHGKYMLLFDPLDGSSNTDNNMPLGAIFSVLKYGRTDRLPTEEELLRRGTEQVAA
                     GYLLYGSSTMLVYTVGQGVYGFTLEPDIGEYLLSHEKIRIPEKGRVYAVNEGNYHKWS
                     EGTKKYLDSLKVSDKTTGRPYSSRYSGCLVADVHRLLLGGGIYLYPGEAGKPEGKLRL
                     LYEANPLAFVVEQAGGRASTGTSRVLEVEPKKLHQRVPLIIGSRQDVEQAEAYVQGKA
                     "
     gene            1934..2857
                     /locus_tag="VN647_02540"
     CDS             1934..2857
                     /locus_tag="VN647_02540"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWG95938.1"
                     /translation="MGDRVKEILSWYGSDNAGTKTNIARLLRSGKLAGTGKLVILPVD
                     QGFEHGPARSFAANPPAYNPHYHFQLAIEAACNAYAAPLGFIEAGAGEFAGQIPLILK
                     LNNHDVLHDDKDPQPSVTGSVKDALRLGCSAVGFTIYPGSAHCNAMYEQLRAIAEEAK
                     AGGLAVVVWSYPRGAGLSKEGETAIDVVAYAAQIAAQLGAHVIKVKLPSAHLEQSAAK
                     KVYEATQVPIKTLAERVKHVVQSTFDGRRIVIFSGGAKNEDKNVFEEARAIKDGGGFG
                     SIIGRNSFQRPKAEAVKFLQTIMGIYSGAIQ"
     gene            2891..4450
                     /locus_tag="VN647_02545"
     CDS             2891..4450
                     /locus_tag="VN647_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HWG95939.1"
                     /translation="MDQIDFDRKPQQGTRRWLVAATLLTAGIIIGFVIASDLGWLPTG
                     HAVPDSSSVAQPAQPPPVARPVSTAPQPALAGSSQTFVEIAKSVKPAVVNIYATKSGR
                     SEGSGPAPFDDPLFRKFFGDEFFRKFEHPKDRKERGLGSGVIVESNGLIITNNHVVGK
                     ADEIRVTLSDKREFKAKLIGTDPKTDVAVVKIDATGLPTVTWADSDKLEVGEFVLAVG
                     NPFGLTQTVTLGIVSALGRAAGIAEYEDFIQTDAAINPGNSGGPLVNVRGELVGMNTA
                     IFSQSGGNMGIGFAVPSNMAQSVMNQLVQTGKVVRGWLGVSIQELTPELASQFGITET
                     KGILVSDVMDDSPAKKAGFERADVIVEYDGKSMDSPTHLRNAVAQTPVGKKVTIKFIR
                     DKKPKTVDLTIVEQPKSMSQSGEDDGGESATPVGVLSSLDVRDLTQELASRYGLKSSE
                     RGVVIVRVKPGSTAEELGVREGDVILEVNRQTVTSVKVFERIAGKLPKDQAVLLLLKR
                     QGRTIYLTLRP"
     gene            4570..>5070
                     /gene="ispD"
                     /locus_tag="VN647_02550"
     CDS             4570..>5070
                     /gene="ispD"
                     /locus_tag="VN647_02550"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWG95940.1"
                     /translation="MALVPAAGRGLRMGGSVPKQFLALGGQPLILHSLHVLQASSVIE
                     EIILAVPQNEMDYSLKEIVAKHRFTKVTKVVPGGRERQDSVRHALEAVHDDVDVVLVH
                     DAVRPFLTGHMVEEVVKTARAKGAAIIALPMKDTVKQVGADHVIERTVDRQSLWLAQT
                     PQAFRRD"
BASE COUNT         1182 a         1258 c         1522 g         1108 t
ORIGIN      
        1 tgttgacatc tgtccctacc tggtctcttc gcccgcccag tgttgctcag atgtgccggt
       61 atgtcgtcct tgtcgctgtg gctgctctgt catcatactc tgccggctcc attgaagctg
      121 agcctctcgg cgaaacgaag cgtccgtttc aggttggaga acggctgaca tatgaggtct
      181 cctggctcaa cctgaccgca gtcatcgcgg tgatggaggt ggcccgaata gatgggcgaa
      241 aggatcaggc cgtcgcgaag ctggtgggga ccgcacaatc aacaccggtt atcaccaagt
      301 ttttccctgt cgacaatcga gtggaatcgg aactggactt ggatactctg actccggaac
      361 atatgacgtt ccgtcggcgc gaaggcaaaa agaaagaaga tatcgagtat gtctttcatc
      421 aaaaagaggg cgtggtaacg gccgtcagag gaggaacgac ggagtcgctc cctatcccgg
      481 tcgggacaca ggatatcatt tcatgcttgt actatacgcg tacgcggttg ccgccgaatc
      541 cgggtgcctc gttgaaaatg aatgtgtatc atgacaaaaa aaatcgtccg gtcgaggttc
      601 gtgtcgaggc tatcgagacc attgaggggg catggggaag ggcggagact gtccgagtgc
      661 tcgtgatcat gccgtttcat ggcctcttca tgaataaggg gaatatccgc gtgtgggtca
      721 cgaatgatga acgaaagacc cctgttcgga tgacagcgaa ggtcgtgctc ggatcgatcg
      781 tcgcagacct ggtcgatggc tttgaggaaa gcggcaccgc cgagcaaaag tattgaacat
      841 tgatgattgt tcgcacgggt caaacccgtt atactgagcg tcagtatgcg agaatttccc
      901 cttaccttag gccgatttat cattcagaat caaacctcgc atcaagatgc gacggaagaa
      961 ttctccagtt tgttgactca gattggtctg gtcggcaagc tcatttcgca agatcttcgg
     1021 cgtgccggac tgatcaacat tcttggaacg accggtgaca cgaatgtgca aggagaaacg
     1081 gtcaagaagc tggacgccat cgccaacgac gacttcgtca aagtctttca acacagcggg
     1141 tatgtctgcg ccttggcctc ggaagaaatg gaaaaaccga tctcgatccc ggggaattgg
     1201 ccgcacggca agtacatgtt gttgttcgat ccgctcgatg gctcctccaa cacggacaac
     1261 aatatgccgc ttggggccat tttttctgtt ctcaagtatg gcaggaccga tcgtttaccc
     1321 actgaggaag aattgttacg ccgagggacc gaacaagtcg cggccgggta tctcctgtac
     1381 ggatccagta ccatgttggt gtataccgtg ggacaaggtg tgtatggatt cacgctcgaa
     1441 cccgacatcg gagaatatct cctatcgcat gagaagatca ggattcccga aaaggggagg
     1501 gtctacgctg tcaatgaagg gaactaccac aagtggtcgg agggaacgaa aaaatatttg
     1561 gattcactca aagtcagcga caagacgaca ggccgtccgt acagcagcag gtattcgggc
     1621 tgtctggtcg ccgacgtgca tcgtctgttg ctcgggggag ggatctatct ctatccgggt
     1681 gaagccggta aaccagaagg aaagctgcgg ttgctctacg aggcgaatcc gttggcgttt
     1741 gtcgtcgagc aagccggcgg aagggcctcg acggggacat cgagagtgtt ggaagtggag
     1801 cccaagaaat tgcaccagcg cgtgccgttg atcatcggaa gccgacaaga tgtcgagcaa
     1861 gctgaagcgt acgttcaggg gaaagcctag attgcacatt tatgtggtac tgtgatgttg
     1921 tgtggaggga attatgggag accgggttaa agaaattctg agttggtatg gcagcgacaa
     1981 tgccgggact aaaaccaaca tcgctcgttt gcttcggtcc ggcaaattgg ccgggaccgg
     2041 caagttggtc attttaccgg tggatcaagg gttcgagcat ggtcccgcgc ggagttttgc
     2101 ggcgaatccg cccgcgtaca accctcacta ccacttccag ctcgcgatcg aggctgcgtg
     2161 taacgcctat gccgcgccat tgggatttat cgaagccggg gcaggtgagt tcgcgggtca
     2221 gattcccctc attctcaagc tcaataatca cgacgtgctg catgacgaca aagatcccca
     2281 gccgtcggtt acgggcagtg tgaaggatgc gcttcgtttg ggttgttcgg ccgtcgggtt
     2341 cacgatttat ccaggctccg ctcactgtaa cgccatgtat gagcagctgc gggccattgc
     2401 cgaggaagcc aaggcaggcg gtcttgccgt tgtcgtctgg tcctatcccc gtggagcggg
     2461 cttgagcaag gaaggtgaga cggccattga tgtcgtcgcg tatgcggcac agattgcggc
     2521 tcaactcggt gcccatgtga tcaaggtcaa gctcccgagt gcgcatctcg aacaatcggc
     2581 ggcaaagaag gtctatgagg ccacacaagt tcccataaaa acattggcag agcgtgtgaa
     2641 gcatgtggtc cagagcacct tcgacggtcg tcggatcgtg attttctcgg gaggagcgaa
     2701 aaacgaagat aagaatgtat ttgaagaagc ccgtgccatt aaggatgggg gtgggttcgg
     2761 gagcatcatc ggccggaact catttcagcg tccgaaggcg gaagccgtta agttcctcca
     2821 aaccattatg ggaatttaca gcggagcgat tcaatgagct cgcgcagctt tgataaagtg
     2881 aatcgacgcc atggatcaaa tagattttga ccgcaaaccg cagcagggaa ccagaaggtg
     2941 gcttgttgct gcaaccttgt tgacggctgg gatcatcatt gggtttgtga ttgcatcgga
     3001 ccttggctgg ttacctaccg ggcatgcggt ccctgattct tcttccgttg ctcagcctgc
     3061 tcagccccct cccgtcgcca gaccggtctc gaccgctcca caacctgcat tggccgggag
     3121 tagtcaaaca ttcgtagaaa tagcaaaatc agtcaagccg gcagtggtga atatatatgc
     3181 gaccaaaagc ggacgttctg aaggctccgg cccggcgccg ttcgatgacc cattgtttag
     3241 aaagtttttc ggcgacgagt ttttccggaa gtttgaacac ccgaaagatc gaaaagagcg
     3301 aggacttggg tccggcgtga tcgtcgaatc aaatggactc atcatcacca acaatcatgt
     3361 ggtcggcaag gcggatgaaa ttcgtgtcac cctgtcggat aagcgcgaat tcaaagcgaa
     3421 gctgatcggc accgatccaa agaccgatgt ggccgtcgtg aagatcgacg cgacggggct
     3481 tccgaccgta acctgggccg attcggacaa actggaagtc ggagagtttg tcctggccgt
     3541 cgggaatccg ttcgggctga cccaaaccgt caccttgggg atcgtcagtg ccctagggcg
     3601 agccgcaggt attgccgagt acgaagattt catccagacg gatgcggcca tcaacccggg
     3661 caactccggt ggacccttgg tcaatgtgcg gggcgaattg gtcgggatga ataccgctat
     3721 attcagtcag agcggaggca acatggggat cggttttgcc gtgccgagca atatggctca
     3781 gtccgtgatg aaccagctcg tacaaacggg aaaagtcgtc cgcggctggc ttggagtatc
     3841 tattcaggag ttgacgccgg aattggcatc ccaatttggt atcacggaga caaagggtat
     3901 actcgtgagc gatgtcatgg acgacagccc ggcgaaaaag gccggattcg aacgggccga
     3961 cgtcatcgtc gagtatgacg gcaaatccat ggattcaccg actcacttgc gcaatgcagt
     4021 ggctcaaacg ccggttggaa aaaaagtcac catcaagttc attcgcgata aaaaaccgaa
     4081 aacggtcgac ctcaccatcg tcgagcagcc caagtcgatg tcgcaaagcg gtgaagacga
     4141 tggaggggaa tcagccacgc cggtcggcgt gctctccagc cttgatgtcc gagacttgac
     4201 ccaagaattg gcgagccggt acggactgaa gtcgagtgag cggggcgtgg tgatcgttcg
     4261 ggtcaagccc gggagtacgg ctgaagaatt aggtgttcga gagggcgatg tcatcctcga
     4321 agtgaatcgt caaactgtga cgtcggtgaa ggtttttgag cggatcgccg gtaagttgcc
     4381 gaaggatcag gcggtcttgc tcttgttgaa acggcagggg cggacgatct acctgacgct
     4441 tcgtccatga ggacaggccg aggtgatcag aatgggatgt tggtgtcgag gttgcgcagg
     4501 caccatctgt tcgtacgctt cccgcgatga gtaaggatag tctagactcc agttgtgaat
     4561 aaccacaccg tggctcttgt ccctgccgcc ggacgaggcc ttcgcatggg cggctcggtg
     4621 cccaaacaat tccttgcact cggcggtcag cctctcatcc tccattcgct tcatgtgctc
     4681 caggcttcgt ccgtcatcga ggaaatcatc ctggctgttc ctcagaacga gatggactat
     4741 agtctcaaag agatcgtggc gaagcatcgc tttaccaagg tcactaaagt ggtgccggga
     4801 ggacgcgaac ggcaagattc tgtcaggcat gcgcttgagg cggtccatga cgatgtcgat
     4861 gtggtgttgg tacatgatgc ggtgcgtccg tttctcacag ggcatatggt ggaagaggtt
     4921 gtgaagacgg cacgggccaa gggagcggcg attatcgccc ttcccatgaa ggataccgtc
     4981 aagcaggtag gagccgatca tgtcattgag cgcacggttg atcggcagtc attgtggttg
     5041 gctcaaaccc cgcaagcctt ccgtcgcgat
//
LOCUS       DATNDD010000107         6553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2583107, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000107 DATNDD010000000
VERSION     DATNDD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583107"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VN647_02555"
     CDS             complement(<1..620)
                     /locus_tag="VN647_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95941.1"
                     /translation="MTRRHRWGWGLLACLLLVFMQDDSWADAPLASTRTNQQAERSAV
                     PDTPATAEAGLSTGEPEGATEELIAGRGFQKFQYKGISFAPGGFFDGTAIVRSANENA
                     DIQSTFANIPLQGSANSHLREFRGTARGSRLNLLSEGHYRSTAIAGYVELDFLGSSTS
                     GNELETNSWNPRLRQLWSNVDLPNGISLLFGQSWSLLTTHRIGLKP"
     gene            complement(617..760)
                     /locus_tag="VN647_02560"
     CDS             complement(617..760)
                     /locus_tag="VN647_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95942.1"
                     /translation="MCQYLILDSGITGYCDGIFATHDEADAAAIQLAKYRIDSRQWES
                     GGP"
     gene            complement(948..1283)
                     /locus_tag="VN647_02565"
     CDS             complement(948..1283)
                     /locus_tag="VN647_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95943.1"
                     /translation="MIRPSKIVYILACGVALGIGLSYQPTWAEDGMKTPHSESRIGGQ
                     AGQPYDQVTYKDESAHTKERLHRQDGERIGGQAGQPYDHLKHDYESTHAKGRVGGEAG
                     ESYSPIQLE"
     regulatory      complement(1478..1548)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     regulatory      1698..1762
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1848..3053
                     /locus_tag="VN647_02570"
     CDS             1848..3053
                     /locus_tag="VN647_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWG95944.1"
                     /translation="MESIFFTATLWLALAVASALVASSLRLSIALVEICVGVVTAAGL
                     EFFEFSNVLAADSEWVKFLASSGAVVLTFLAGAELDPAIVRMKLTEVTVVGLVGFMAP
                     FLGCAAVAYYLLGWDANASWLCGVALSTTSMAVIYAVMLETGFNKTEFGKGILASCFV
                     TDLGTVIALGLLFAPFTYKTLVFIAGIIVALITLPTITAWLTRHYAHRTAAIRTKWVM
                     LTLFGLGALALWSGSEAVLPAYLAGMVLAGSAARDTHWIRRLRTLTVGFLTPFYFLRA
                     GTLVSLPALLAAPFVFLLLLIGKVASKIVGLYPFISLFRQDRKERWYYTLLMSTGLTF
                     GTISSLYGLAHGLVTQEQYSFLVAAVIASAVVPTLIAGLFFVPTHLLPKADHGKRAVR
                     RINDLSDEG"
     gene            3135..4319
                     /locus_tag="VN647_02575"
     CDS             3135..4319
                     /locus_tag="VN647_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG95945.1"
                     /translation="MKRSQSHIPSGVWVLGFVSMLMDISSEMIHSLLPLFMVTILGAS
                     AIAVGLIEGSAESLALVVKVFSGTLSDYLGRRKGLAVFGYALGALTKPLFALASGVGM
                     VLTARLLDRVGKGVRGAPRDALVADIAPPQLRGAAFGLRQSLDTVGAFVGPLVAVGLM
                     LLWADDFRAVFWVAVVPGLMAVALLVFGVREPVRRQTETRTNPIRRDNLRRLDSAYWW
                     VVGIGSVFTLARFSEAFLVLRAQQGGMPIALVPLVMVAMNLIYALSAYPFGKLSDTMS
                     HTRLLVSGLIVLIAADVVLAINNHWGMVLVGVVLWGLHMGMTQGLLAAMIAGASPADL
                     RGTAYGFFNLACGLALLIASALAGIVWEEFGARFTFYVGATCAACAIALVAVKADQSS
                     SA"
     gene            4379..4708
                     /locus_tag="VN647_02580"
     CDS             4379..4708
                     /locus_tag="VN647_02580"
                     /inference="COORDINATES: protein motif:HMM:NF002586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HWG95946.1"
                     /translation="MSVVNSLGLFVLAGLCEIGGGYLVWLWFREGRPLAYGIAGAAIL
                     ILYGIIPTFQPAHFGRVYAAYGGMFIFLSLFWGWALDGVRPDQFDIIGAAICLIGMAV
                     IMYAPRS"
     gene            complement(4878..5108)
                     /locus_tag="VN647_02585"
     CDS             complement(4878..5108)
                     /locus_tag="VN647_02585"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWG95947.1"
                     /translation="MPKKTQSVSRSTQRDAGVESRSNGNGVTDRPDEDSHGIHARISE
                     RAYALYEEHGRKDGHALEDWLAAEQHILNQAV"
     gene            complement(5188..5595)
                     /locus_tag="VN647_02590"
     CDS             complement(5188..5595)
                     /locus_tag="VN647_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95948.1"
                     /translation="MMTSRQHGERTRVMIVEQDLEFGLKLADWLATHGYHPAFISTVN
                     AAIDGLAGFRPQAVFVGLGCSAPAAHVNMVEILLLIQTICPSVPVIMMADQIHEDLTQ
                     VVFRQGVRRLLAKPVEFSHIGEVLQSELSAATV"
     gene            complement(5641..6030)
                     /locus_tag="VN647_02595"
     CDS             complement(5641..6030)
                     /locus_tag="VN647_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95949.1"
                     /translation="MSIGSIRELGYTSAIHVVQQIAYCFRLTKTENVTAETAIPTVDS
                     VMCREITGVGPCTFDELVHRLPTYSWAQVFSAVDRLSRQGTLTVSRTRRFGYVVSVGP
                     APLIPESSQDRECRAVRPQVIRYGAAA"
     gene            complement(6206..>6553)
                     /locus_tag="VN647_02600"
     CDS             complement(6206..>6553)
                     /locus_tag="VN647_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG95950.1"
                     /translation="DGYLTKSSAPEELVKAITKVRGGGKYVSPSLAEALIVTIRPGAA
                     KEPHQWLSDREYQVLCFIGSGKTVGEIAGRMNLSVSTISTYRARILEKMGMKTNAELT
                     RYVLQHGLAEGAC"
BASE COUNT         1276 a         1818 c         1851 g         1608 t
ORIGIN      
        1 ctcggtttga gcccgatgcg atgggtggtg agaagagacc agctttggcc gaacaacagg
       61 gagatgccgt taggaagatc gacattgctc cacagctgcc gcaatctcgg attccaacta
      121 ttggtctcca actcgttccc ggacgtggag ctcccgagaa agtccagttc gacatagccg
      181 gcgatggccg tgctccgata gtgtccttcg cttaacaagt tcagccggga accacgggcc
      241 gtcccccgaa attcccggag atggctattg gctgaccctt ggagcgggat gttggcaaac
      301 gtactttgaa tgtcggcatt ctcattggcc gatcggacaa tcgcggttcc atcgaaaaac
      361 ccgccagggg cgaaggaaat gcccttgtac tgaaatttct ggaatccccg tcccgcaatc
      421 agctcctcag tcgccccttc tggctcgccc gttgagagac ccgcttctgc ggttgcaggc
      481 gtgtcgggca cggctgagcg ctcggcctgt tggttcgtac gcgtagaagc cagaggggca
      541 tccgcccatg agtcatcctg catgaacacg agcagcaagc acgcgaggag tccccacccc
      601 catcgatgac gcctcgtcat ggacctcccg actcccattg gcgggaatcg atccgatact
      661 tcgccaattg gatcgcggcg gcgtcggcct cgtcatgcgt ggcaaagatc ccatcgcagt
      721 agcccgtaat gccggagtca agaatgagat actggcacat ccatcgccca tctttctgtt
      781 gacccgctgt taagcggtca ggtggccttt atattctcgt aggatggctt cctcctcacg
      841 aatccacgcc attgagatcc tagagcccaa ttgcgcggga taggcgttgg cgtcattcct
      901 gctctcgaca acgacccacc agaatttcct ttcgactgcg aagcaggcta ttctaattga
      961 atgggactat agctctcgcc tgcttcacct ccgactcgtc ctttcgcgtg agtcgactcg
     1021 tagtcgtgtt tcaggtgatc gtagggttgc cccgcctgtc cgccaatgcg ttctccatcc
     1081 tgtctatgca gtcgctcctt cgtgtgggca gattcatctt tgtaggtgac ctgatcgtag
     1141 ggttgtccgg cttggccacc gatacgtgat tcagaatgtg gggttttcat tccgtcttcc
     1201 gcccatgtgg gttgatagga gagaccaatg cccagggcaa ccccacacgc gagaatataa
     1261 acgattttag atggccggat catgagaacc tccttggttg ggataacggg cagggaagac
     1321 catcctcctg ccttgatgca gccaaaaagg acgtattgat gtgagctcag aacagagcag
     1381 cgtttctcag gggaatgtga gaaggaaagg caactggaag aagcggaaga agctcgaccg
     1441 gggaacccca taaaaaaact cctaccagac cagattgtgt caggtaggag ttatcattcc
     1501 cctccggaga gaggacggtt cttggtgaac tccatcacca cgtcgattta ttcagggaaa
     1561 agcaagtctc gcgccacaca aaagcatgac gaaacgggtc gattctcctg ctcaagcaaa
     1621 caatcgagct tttttgcaca gggtgcatcg tgcaaaatgt cactcattcc agtatgctta
     1681 ttttcccagt atactcatcg ttcgcagggg atggagtccc ccgtataagt gccttggcca
     1741 ggctgatgac tcctacgcta ctactgccgt agccgtagga ttgtcgtcga tcagatggcg
     1801 accggctcaa agcagtatgc gtcttggccg gaacggagga ctcatcaatg gaatcaatct
     1861 ttttcacggc aaccctgtgg ctcgccctcg ctgtcgcctc tgcactcgtc gcttcctcgc
     1921 tgcgtctctc catcgcatta gttgaaatct gtgtgggagt cgtcaccgct gcgggactgg
     1981 agttcttcga gttcagcaat gttctcgccg ccgattcgga atgggtaaag tttctcgcct
     2041 cgtccggcgc cgtcgtcctc acatttctgg ccggtgcgga gcttgatccc gccatcgttc
     2101 gcatgaagtt gaccgaagtc actgttgtcg ggttggtggg attcatggcg ccgttccttg
     2161 gctgcgccgc cgtcgcctat tacctcttgg ggtgggacgc gaatgccagt tggctctgcg
     2221 gtgtggccct ctccacaaca tccatggcgg tgatctatgc agtcatgctg gaaaccggtt
     2281 ttaacaagac ggaattcggc aaaggaattc tcgcctcatg cttcgtcacg gacctgggaa
     2341 cggtgatcgc cctcggactg ctctttgcgc cctttaccta caagactctt gtatttatcg
     2401 cggggatcat tgtcgcgctg ataacgctgc ccaccatcac cgcgtggctg acacgacact
     2461 atgcgcatcg cacagctgcg atcagaacga aatgggtcat gctgacgctt tttggattgg
     2521 gcgcattggc gctgtggtcc ggaagcgaag cggtgttgcc ggcctatctt gccggcatgg
     2581 tcctcgcggg aagcgccgcg agggatacgc attggattcg tcgattgagg accctgactg
     2641 tgggattcct cacgcccttt tatttccttc gcgccgggac attggtgtcc ctacccgctc
     2701 tccttgccgc tccattcgtg tttctcctgt tgctcatagg gaaagtggcg tcgaagattg
     2761 ttggcctcta tccctttatc agcttgttcc gtcaggaccg caaggaacgc tggtattaca
     2821 cactattgat gtcgaccggc ctgacgtttg gaacgatttc ttccctctat ggccttgcac
     2881 acggattggt gactcaagag cagtattctt tcctcgtggc ggcggtcatc gcgagcgcgg
     2941 tcgtgcccac tctcattgcg ggtctgtttt ttgttccgac acacctcttg ccgaaggctg
     3001 atcatgggaa acgagcagtg agacggatca atgacctaag cgatgagggt tagatgcgaa
     3061 accgacatgc cgcatcatct gctttcatcg tcgcctttac tgggattagc gattaatcga
     3121 tccacccctt acagatgaag cgctcacaaa gccatattcc gtccggcgtc tgggtgctgg
     3181 gcttcgtcag catgctgatg gacatttcat cagaaatgat tcatagcttg ctgccgttgt
     3241 tcatggtcac gattctcggc gcaagcgcaa tcgccgtcgg ccttatcgaa ggatcggccg
     3301 aatcccttgc tctggttgta aaagtatttt ccggcacgct gagcgattat cttggaaggc
     3361 gtaagggact ggccgtgttc ggctatgcct taggcgcgct gaccaaacct ctctttgcgc
     3421 tcgcctcggg agtgggcatg gtattaaccg cgcgattgtt ggatcgtgtc ggaaagggcg
     3481 tgcgtggtgc accgcgcgat gcgttggtgg cggacatcgc accgccgcag ctccgaggcg
     3541 cagcgttcgg cttgcggcag tcgctggata ctgtcggcgc gttcgttggt ccattggttg
     3601 ccgttgggtt gatgctcctc tgggccgatg actttcgggc agtcttctgg gtagccgtcg
     3661 ttccagggtt gatggccgtg gcactcctgg tcttcggggt ccgagagcct gtacgccgtc
     3721 aaaccgagac gaggacaaac ccgattcgaa gggataatct ccgacgactt gactccgcct
     3781 attggtgggt ggtgggaatc ggctcggtgt tcacgctggc acggttcagc gaggcttttc
     3841 tagtcctccg cgctcagcag gggggaatgc ccatcgctct tgtgcctctg gtcatggttg
     3901 caatgaatct gatctatgcc ttatcggcat atccgttcgg caagctctcc gatacgatga
     3961 gccatacgag gctgctcgta tcaggcctga tcgttctgat tgccgccgat gtggtgttgg
     4021 ccatcaacaa ccactggggt atggtactcg tgggcgttgt cttgtgggga ctgcatatgg
     4081 ggatgacgca aggcttgctg gcggccatga tcgccggtgc ctcacccgca gatctgcgtg
     4141 ggacagcata cggcttcttc aacttggcct gcgggttggc tttgctcatc gctagtgcgc
     4201 ttgcgggcat cgtctgggaa gaattcggcg cacggttcac attctatgtg ggggcaacat
     4261 gtgccgcttg tgcgattgca ctggttgccg ttaaagcgga tcaatcttca agtgcttgaa
     4321 tccacccgcc atcgggattt ccacagcagc agccggtacc cttggaaagg acgcaagaat
     4381 gtcggtcgtt aattcattgg gactgtttgt gctcgccggt ttatgcgaga ttggtggtgg
     4441 gtatcttgtg tggctctggt tccgcgaggg acggccactt gcatacggaa ttgcaggagc
     4501 ggccattctt attctttacg gcattattcc aacgtttcaa cccgcccatt ttggtcgagt
     4561 gtatgccgcc tatggcggca tgtttatctt tctgtcgtta ttctggggat gggctctaga
     4621 tggcgtccgc ccggaccaat tcgacatcat tggagccgcc atttgtctca tcggcatggc
     4681 ggttatcatg tatgcacctc ggagctagcg gcaaaggctc gtgcgcaggg gcggccacat
     4741 ctccattcgg cattcccact ccgcgaatga acactgggtt gccggacttg tcggcagaat
     4801 gcaggttacg aactgtccgt tcccattcat ttcatggcag gcttgaagat gccttggttg
     4861 gatggtccaa catcctgcta cacagcttga ttgagtatgt gttgttcggc cgccaaccag
     4921 tcttcgaggg cgtgtccgtc ctttcggcca tgctcctcat acagagcgta ggcgcgttcc
     4981 gagatgcgcg catggatacc gtgggaatct tcatcaggcc ggtcggtaac gccgttcccg
     5041 ttgcttcgtg attcgactcc tgcgtcacgt tgtgtggagc gtgagactga ctgagttttc
     5101 ttgggcatga cgggctctcc tcctctttat aacaacgctt acagtaatct tgcgcttctc
     5161 tacgggttgg gcgacgattt caccgattta taccgttgcc gcactcagtt cagactgaag
     5221 cacctcgccg atgtgcgaga attccaccgg cttggccagc aaacggcgaa ctccttggcg
     5281 aaacacaact tgcgtcaggt cttcgtggat ttgatccgcc atcatgatca ccggaacgga
     5341 gggacaaatg gtctggatca ggaggagtat ctcaaccatg ttgacatggg ctgcgggcgc
     5401 agaacagccg aggccgacaa agactgcctg cggcctgaaa ccggccaagc cgtcgatcgc
     5461 ggcattgact gtactgatga atgccggatg atagccgtga gttgcgagcc aatcggcaag
     5521 tttcaatcca aattccaagt cctgttccac aatcatcacc ctggttctct caccgtgctg
     5581 ccggctcgtc atcatccgat ctcctttcgc ttctgcctta gcctcatgcc gcttcacagt
     5641 tcacgcggcg gcgccgtatc ggatcacctg aggtcgtacc gctcggcact cccggtcttg
     5701 cgaagactcg ggaatgagag gagccggtcc cacagatacc acgtagccga agcggcgcgt
     5761 acggctgact gtgagggttc cttgccggct gagccgatcc accgccgaaa acacttgcgc
     5821 ccaggaatag gtcggcagcc ggtgaaccaa ctcatcgaag gtacagggcc caacaccggt
     5881 gatttctcga cacatgactg agtcaacagt ggggatagcc gtttctgccg taacattctc
     5941 cgttttggtg agacgaaaac aatatgcgat ctgttgcaca acgtgtatgg cagatgtata
     6001 gccgagttcc cttatggacc ctatcgacaa gaactcgatt ttcttgtcga atcgtcaact
     6061 gcgcactgtc gtggttctta cgacagagga gagaagaact gttgagatgg gagtactgag
     6121 tgttcagcgc ggaagccgga gagggcagag atcagggacc aaagggtggc aggactgtac
     6181 gcgcatatct cgttcaacac aatactcagc atgctccctc cgcgagtcca tgttgcagca
     6241 cgtatcgggt gagctccgcg ttggtcttca tgcccatctt ttcgagaatc cgtgcgcggt
     6301 aggtgctgat tgtcgagacg ctcaggttca ttctcccggc gatctcgccg acggtttttc
     6361 ccgaaccaat gaagcacaac acctggtatt cccgatctga aagccattgg tgaggctcct
     6421 ttgcggcgcc gggcctgatg gtgacgatga gggcctccgc cagagaaggg ctcacatatt
     6481 tgcctccacc ccgtaccttc gtaatggcct tgaccagctc ctccggcgcg ctggatttgg
     6541 tgagataccc atc
//
LOCUS       DATNDD010000108         6952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1779824, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000108 DATNDD010000000
VERSION     DATNDD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779824"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VN647_02605"
     CDS             <1..476
                     /locus_tag="VN647_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95951.1"
                     /translation="QHAVKIKRQEAGWGYSLVLYAGMLGTIAVGLWANGKESVDGAMT
                     AFGWVYNFMMVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLVAAVIVMMGRVPLG
                     EYLIPLSGDVSSWILNVLNSSVRRAILIGVSLGAVALSFKIIFGVERSYLGGGKE"
     gene            563..1393
                     /locus_tag="VN647_02610"
     CDS             563..1393
                     /locus_tag="VN647_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95952.1"
                     /translation="MTLSERMLKIDRRIIFLVIGLCTLLPLLYPVGLPIKITSEVRGV
                     YDYIESLPERSVFLLSIDFDPASKPELHPQAIALLRHAFRKNLRVIAMTLWVSGTGMA
                     DQLVTQVAKEMGKEYGKDYVFLGWSPGGQAVIINMGQNLYSAFPSDYSGKPTKDLPIL
                     ADVRNLKDVAYMVSLGAGKPGVEEWYVFGKDKYKFEMGGGCTGVMAPGLYPLLRSGQI
                     NGLIGGLRGAAEYESLIGQKGKAVAGMDAQSATHVAIIMLVIMCNLFYFSLRQQRGQG
                     "
     gene            1416..2177
                     /locus_tag="VN647_02615"
     CDS             1416..2177
                     /locus_tag="VN647_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95953.1"
                     /translation="MSFELIFGAWVATGLTLLIFSFLYKDNPLFKLAEHLYVGVSVGY
                     IIVKTYDTVIVHLVVKPIVENGEVALLIPVAIGMLMLTRYVPRAAWMSRYAFAFIVGM
                     GAGLAIPRTISSFILKQIEDTVRPLLSMTGPDGVAFSMNLLNPASNLNALIILLGVGS
                     VLFYFFFSIEHSGPGRAVARTGVIFLMISFGAGFGYTVMARMSLLIGRLTDLIEFSDA
                     SYGRPTIWLTLLTIGALIVISVVSRRARSEPPQEG"
     gene            2296..3531
                     /gene="amrB"
                     /locus_tag="VN647_02620"
     CDS             2296..3531
                     /gene="amrB"
                     /locus_tag="VN647_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HWG95954.1"
                     /translation="MTIGVAKDSANYPVLRNLQFSPIKQGEDQLIVLWDPSGLSKEKL
                     VLPLNFFFIVQHFDGAHSLQEIGALYLKRFGEFLLPSKVEQLVVDLDQKLFLEGPRAE
                     TARQQAQIDYRQQPVRPAAFAGRSYEADSAKLRKQIDGFFTSGEGPDFKPSENRGKLI
                     KGLVVPTYDLKQAGPVYAWGYKELQEAQQPDSYVLIGTAHAGLENLFAVTDKDFETPL
                     GMAHVDRTIVNQLKGMVPKYFEEDIAHQSEHAIEFQLPFLQTTVGKPVAIVPVLSSFS
                     ALSLNDPTVRNSVDRFLNALKDVIVASGKAVCVIAAGELAHLGLRYGDSDPPTDFSFH
                     RSMQRDLEMLKPVEELKPEEFSGFVQQENDQRRISGFSPIYSLLRLIQAEKGHVLRYD
                     RGITDQYNSTVTYASMAFF"
     gene            3708..4427
                     /locus_tag="VN647_02625"
     CDS             3708..4427
                     /locus_tag="VN647_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95955.1"
                     /translation="MARHWSSILFVLGFLLVELSDVVFRQATAESLMGGDQHQLQVLL
                     SQKNERVFDPDFLLRLADLYLDLGDDDSLATSKRRDAYEEGAKVARQAIKLREQDAQA
                     HYLYAANLGSAAQLKGMMASALTIQDLKHHVTRALELNPHHAPALHMMGMMLEELPWF
                     LGGDANSALTYLRRAVISDPKDCHARLDLAKTYIKRKDQNSALRELETILQQPHQPDA
                     SISDKRHREEALQLRASLKNS"
     gene            4494..5855
                     /locus_tag="VN647_02630"
     CDS             4494..5855
                     /locus_tag="VN647_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG95956.1"
                     /translation="MGSRRLKLFEVFKATTLGILAILLLFVGSATHPTTISAYLLFFA
                     IMVLQGIEIFAPSFSSNTLDTKYRTLLLRLSILAQLIFASSLVAVTDGSGSIYELVYL
                     LPIVSAATKLPGRDVVFVVGAAVISMIGFIVAGETLTPSVVHVKAFQDAVSATVYFTM
                     AGLLVYFFAEDERKQRQKYQDMAAALVESNSKLQTAQAQLWERLDQVTKMEQRLQEIS
                     QMAVLGEVAGQIAHEVRNPLGIIKCSAEMLAARTKDSSTQRHIQVLLEETTRLDKAVE
                     GVLRLGSPLRINKAVVNVSELLNSVVQLSSAWSLHPRVSIDVTEPAADRLLEGDYDLL
                     HQALSNLVRNACQAMPSGGVVKICCASHDDNPRFLISIVDTGVGMSDEDLRHLGEPFF
                     TKRRGGVGLGFSFARRVIEEHGGVLEVISVQNRGTTVTASLPRFVHNLPKHLLGAGGQ
                     RGV"
     gene            5855..>6952
                     /locus_tag="VN647_02635"
     CDS             5855..>6952
                     /locus_tag="VN647_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWG95957.1"
                     /translation="MAVILVVDDEKNYLWMIEELLQGEGLDVVTCKNGTDALIVLREA
                     MIDVLLTDLRMAEMDGLSVLEKAREISPATSTLLMTAYGTIERAVEAMRCGAYDFIVK
                     PFENAHLIRTIHRAIERSVLVRENVRLSSSLARQFHPDRLIGRSAGMLGVYNKIHRVA
                     DSKSAVLIGGESGVGKELVARAIHFNGPHSGCPFLAVNCSALTDSLAESELFGHEKGA
                     FTGALSRHLGLFEQANGGTLFLDEIADLPMPLQAKFLRVLDSQEIRRIGSEKTFHVDV
                     RILAATHRDLKSAVKEGRFREDLFFRLNVVQIDIPPLRERADDIPLLAEAYLHGLVRE
                     EKVRGKQFSQATIELLKQYKWPGNVRELQNAI"
BASE COUNT         1565 a         1716 c         1944 g         1727 t
ORIGIN      
        1 atcaacatgc ggtcaagatc aaacggcaag aagcaggatg gggctatagc cttgtcttgt
       61 atgccggcat gcttgggacg attgccgtcg gactgtgggc taacggaaaa gaaagcgtcg
      121 atggcgccat gacggcgttc ggctgggtct acaacttcat gatggtgccg ctgcaaggca
      181 cgatgttcgc catcctggcc ttcttcatcg catcagccgc ctaccgttcg tttagagcca
      241 gaagtcgaga agcggcggtg ctcttggtgg cggccgtgat cgtgatgatg ggacgggtac
      301 cgctgggtga gtatctgatt cctctgagcg gcgatgtgtc gtcgtggatt ttgaatgtac
      361 ttaattcctc cgtgcgccga gccattttga tcggggtcag tctcggagcc gtcgccttgt
      421 cgtttaagat catcttcggc gtggaacggt cgtaccttgg cggtgggaag gagtaggaag
      481 tgcgggaccc tttcgctgcc cgcgaccggg cttctgctcg gcgccaccca cagttcatga
      541 ctgaggggga actggaaaag gcatgactct ttcagaacgc atgctcaaga tcgatcggag
      601 gatcattttc ctggtgatcg gtctctgtac gctcttgccg ttgctctatc ctgtcggttt
      661 gccgatcaag attacctcgg aagtgcgcgg ggtctacgat tacattgaat cattaccgga
      721 gcgttcggtc tttctcctgt cgattgattt tgaccctgcc tctaaacctg agctccatcc
      781 gcaggcgatc gcgttgctgc ggcacgcctt cagaaaaaat cttcgagtga tcgccatgac
      841 actgtgggtg tccggtaccg ggatggccga tcagttggtt acacaagtcg cgaaagagat
      901 ggggaaagaa tacggaaaag actatgtctt tctcggatgg agtcccggag gccaagcggt
      961 gatcatcaat atgggacaga atctctattc ggccttcccc agcgattaca gcggcaagcc
     1021 tacgaaggat ctgccgattt tggctgacgt gcggaattta aaagatgtcg cctacatggt
     1081 cagcttggga gcgggaaaac caggcgtgga agagtggtac gtcttcggca aggacaaata
     1141 caagtttgaa atgggcggcg gatgcacggg agtcatggct cctggactgt atcctttgct
     1201 aagaagcggg cagatcaatg gcctcatcgg cggtcttcgc ggggcggcgg aatatgaaag
     1261 cttgatcgga cagaaaggca aggccgtagc cggtatggat gcgcaatcgg ccacacatgt
     1321 ggcgatcatt atgctggtga tcatgtgcaa tttgttttat ttttctctcc ggcagcagag
     1381 gggacaaggg taaggtcgag gttgaaggga ctcctatgtc gtttgaattg attttcgggg
     1441 cctgggtagc gacaggatta actttgctga tcttttcgtt cctgtacaaa gataaccccc
     1501 ttttcaaact ggcggaacac ctctatgtcg gagtctccgt cgggtacata atcgtcaaga
     1561 cgtacgatac cgtgattgtt cacttggtcg tcaagccgat cgtggaaaat ggtgaagtcg
     1621 cgcttttgat tccggtcgcc atcgggatgc tcatgttgac tcggtatgtg ccgagggcag
     1681 cctggatgtc tcgatatgca ttcgccttta tcgtcgggat gggcgcgggg ttggcaattc
     1741 cccgaaccat ttcttctttc atcttgaagc aaatcgaaga taccgttcgt ccgttgttgt
     1801 cgatgacggg gccggacggc gtcgccttct cgatgaatct gctcaatccg gccagcaatc
     1861 tcaacgcgct cattatcttg ctcggagtcg gctctgtgtt gttctacttc ttcttctcga
     1921 tcgaacatag cgggccggga agggctgtgg ctcgaaccgg cgtcatcttc ctgatgattt
     1981 ccttcggcgc aggattcggc tatacggtca tggctcgcat gtcgctcttg atcggtcgat
     2041 tgaccgattt gatcgaattc tccgatgcct cgtacggacg accgactatc tggttgaccc
     2101 tcttgaccat tggggctctc attgtcatca gtgtcgtcag tcggcgtgcg cgatccgagc
     2161 ctcctcaaga aggttgaggt tgaggataag gttgagtcgg aaaggccttg ccgcttgcat
     2221 cttcaactca gccttgaccg tagccttccg gtcagttgca gtcgctcagg tccgtctcct
     2281 acaatgacca tcgccatgac catcggtgtc gcaaaagatt ccgccaacta tccggtcttg
     2341 cggaatctcc aattctcacc aattaaacag ggagaagatc aactgattgt gctgtgggat
     2401 cccagcggcc tgagcaaaga aaagctggtc ttgccgttga actttttctt cattgttcag
     2461 cattttgatg gggcgcattc actccaagag atcggtgcgc tgtatctgaa acggttcggc
     2521 gagtttctct tgccgagcaa agttgagcag ctggtggtgg atctggacca gaagcttttc
     2581 ttggaaggcc cgcgagctga aacagcgcga cagcaggctc agatcgacta tcggcaacaa
     2641 ccggtccgtc cggctgcttt tgccggacgg agctacgaag cggacagtgc caaactgcgg
     2701 aaacaaatcg acggtttctt cacatcgggc gagggaccgg attttaagcc ttcagagaac
     2761 cgcggcaagt tgattaaggg gcttgtggtg cccacctatg atctcaaaca agcaggaccg
     2821 gtctatgctt ggggatacaa agaattacag gaagcgcaac agcctgatag ctatgtcctt
     2881 attgggaccg cccatgcggg ccttgaaaac ctctttgccg tcacggacaa ggattttgag
     2941 acaccgttgg gaatggcgca cgttgatcgg acgatcgtga accagttgaa gggaatggtc
     3001 ccgaaatact tcgaagaaga catcgcgcat caatcggaac atgccattga gttccaactc
     3061 ccgttccttc aaacgaccgt cggcaaaccc gtcgcgattg tcccggtact gtcgtcgttt
     3121 tccgcattga gcctgaacga tcctaccgtt cggaactcag tggatcgatt cctcaatgct
     3181 ctaaaggacg taattgtggc ctccggcaag gcagtgtgtg tcatcgcggc aggggagttg
     3241 gcccacctcg gcctccgtta cggcgatagt gatccaccca ccgacttctc ctttcatcga
     3301 tccatgcagc gcgatttaga aatgttgaaa ccggttgaag agctcaagcc ggaagaattt
     3361 tcaggatttg tccaacagga gaacgatcaa cgacgtatct ccggcttctc acccatctat
     3421 agcttgctgc ggttgatcca agcggaaaag ggtcatgtcc tccgctacga tcgtggaatt
     3481 acagatcagt acaactcgac cgtcacttac gccagcatgg cgttcttcta gttgaccccg
     3541 ctgaatcgtc agcttaaccc ccaataaagc gctctccatg tcagaacgag gaattcttgc
     3601 tgaacgagtc tctgcaagtt ctcctcgaag catgattgtt atcaaacagg cgaaagttta
     3661 agatcaactc tgatagagaa tcttttattg ggaaataaga acgagtcatg gcgaggcact
     3721 ggagctctat ccttttcgtt cttggattct tgctcgttga gctgagcgat gtcgtatttc
     3781 gacaggcaac cgcagaatca ttgatgggag gcgaccaaca tcagttgcaa gtgctgctca
     3841 gccagaaaaa tgaacgcgtg ttcgatcccg atttcctcct tcgcttggcg gatttgtatc
     3901 tggatttggg cgatgacgac tctctcgcga catcgaagcg aagagacgca tatgaagaag
     3961 gggcaaaagt tgcgcgccag gctattaagc tccgcgaaca ggatgctcag gcgcattacc
     4021 tttatgcggc gaatttgggg agcgcggctc agctcaaagg catgatggcg tccgcactga
     4081 cgattcagga tttgaagcat catgtcacgc gagcattgga gttgaatccg caccatgcac
     4141 cggcattaca tatgatgggg atgatgctgg aagagttgcc atggttctta ggcggcgatg
     4201 cgaatagcgc acttacctat ctccggcggg ctgtaatttc cgacccgaaa gattgccatg
     4261 cgcgccttga tctggccaag acatatatca agcgcaagga tcagaattcc gcccttcgag
     4321 aactggagac cattctgcaa cagcctcatc aaccagacgc atctatcagc gacaagcgtc
     4381 acagggaaga ggcattacaa ctgcgggctt ctctgaagaa ttcgtagaag atacggtgtt
     4441 tttgtgatca tgtctccgtt ggtgtgatcg gtcgttatga ggtatgcaca cgtatgggat
     4501 cgcgccgtct aaagctattc gaagtcttca aggctacgac tctaggcata ctcgccatcc
     4561 tcttgctctt tgtcggcagt gcaacccatc ccaccacgat cagtgcctat ctattgttct
     4621 ttgccattat ggtgcttcag ggaatcgaga tattcgctcc ctcattctca tcgaataccc
     4681 tcgacaccaa gtaccgaact cttttgttgc gtctcagtat tctggctcag ctgatctttg
     4741 cttcgtccct cgttgccgtt acagatggaa gcgggagtat ttacgagtta gtgtacttgc
     4801 tgccgattgt ttctgcggcg acgaaactgc ctggacggga tgtggtcttt gtcgtgggcg
     4861 ctgcggtgat ttccatgatc ggattcattg tggcgggcga gacgttgact ccttctgtcg
     4921 tccacgtcaa ggcattccag gatgcggttt ctgcaacggt ctattttacg atggcagggt
     4981 tgctcgtcta tttctttgcg gaggatgagc ggaaacaacg ccaaaagtat caagatatgg
     5041 ccgctgccct cgtcgaaagc aacagcaaat tacaaactgc ccaagcccaa ttatgggaac
     5101 gtcttgatca agtgacgaag atggaacaac ggctccaaga aatcagtcag atggccgtcc
     5161 tcggtgaagt cgcaggccag atcgctcatg aggttcgcaa tccgctcgga atcatcaaat
     5221 gttcagcaga aatgttggca gcgagaacga aagattcttc cactcaacgc cacatccagg
     5281 tcttgctcga agaaacgact cggcttgata aggccgtgga gggcgtactc cggcttgggt
     5341 ctccgttgag aataaacaaa gccgtggtca atgtgtctga actcctcaac agtgtcgtac
     5401 agctctcttc tgcatggtct ctccatcctc gagtatctat cgacgttact gaaccggcgg
     5461 cggaccgtct tcttgaagga gattacgacc tgctccatca agcattgagc aatctggtgc
     5521 ggaatgcctg tcaggctatg ccgtctggag gagttgtaaa aatctgttgc gcaagccatg
     5581 acgacaatcc acgattcctc atctctattg ttgatacggg tgtgggaatg tcggatgaag
     5641 atctcagaca tttaggtgag ccattcttta ccaagcgcag aggaggggtc ggacttgggt
     5701 tctcatttgc gcgacgggtg atcgaagagc acggtggagt gttggaggtg atcagtgtac
     5761 agaaccgagg gacgaccgtc acagcctctc ttccaagatt tgtacacaac cttcccaagc
     5821 atctgcttgg cgctggaggt cagagggggg tgtaatggca gtcattctcg tcgtagacga
     5881 cgaaaaaaat tatctctgga tgatcgagga actacttcaa ggggaaggcc tcgacgtcgt
     5941 gacctgtaag aatgggacgg atgctctcat cgtgctgcga gaagcaatga ttgacgttct
     6001 cctgactgac cttcggatgg ccgaaatgga tggattgtcc gtactggaga aggcgcgtga
     6061 gatatcccca gccacatcca ccctcttgat gacagcctac ggcaccattg aacgggcggt
     6121 tgaggccatg aggtgtggtg cctatgactt tattgtgaaa cccttcgaga atgcccatct
     6181 catccggacc atccaccgtg cgatcgaacg aagtgtgctg gtacgcgaaa atgtccgttt
     6241 atccagctca ttggctagac agttccatcc tgaccgtctg attggacgaa gtgccgggat
     6301 gctaggagtc tacaacaaga ttcatcgggt cgcggactcg aagagcgcgg tgttgattgg
     6361 cggtgagagc ggagtgggga aggagttggt cgcgcgcgcg attcatttca acggcccaca
     6421 cagtggttgt ccattccttg ccgtgaattg cagtgcgttg actgactcac tggccgagag
     6481 tgagttgttc gggcatgaga agggagcctt tacgggggct ctgtcacgtc atcttgggct
     6541 cttcgaacag gccaatgggg gaacgctttt tctggatgag attgccgatc tccccatgcc
     6601 gctgcaggcc aagtttttga gagtgttgga tagtcaagag attcgccgga tcgggagcga
     6661 aaagacgttt catgtcgatg tccgaatttt ggccgcgact catcgtgatt tgaaatcggc
     6721 ggtgaaagag gggcgattcc gagaggatct cttcttccga ctcaatgtgg tgcagatcga
     6781 tattcccccg cttcgtgagc gggctgacga cattccacta ctggcagaag cttatcttca
     6841 tggtcttgtt cgagaagaaa aggttcgtgg caagcaattc tcacaagcga ccattgagtt
     6901 gctgaaacaa tataaatggc caggaaatgt gcgtgagttg cagaacgcaa tc
//
LOCUS       DATNDD010000109         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3532721, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000109 DATNDD010000000
VERSION     DATNDD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532721"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VN647_02640"
     CDS             complement(<1..489)
                     /locus_tag="VN647_02640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWG95958.1"
                     /translation="MSDGSVDLSIVIVSYNTRVLLLDCLTSLFESTLATRFEVIVVDN
                     DSKDGTVEAVRTNYPTVRVIVNRENRGFARGVNQALAVSAGRYLLMLNSDTLVRRQAL
                     DRMVACLDGDPRIGAVGCKQWTENGRLYQSCFPFPSIQDHLIHASFLRKFASAWLEAL
                     AAR"
     gene            complement(489..1976)
                     /locus_tag="VN647_02645"
     CDS             complement(489..1976)
                     /locus_tag="VN647_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWG95959.1"
                     /translation="MTDLSSMSIQDAESSPLRLRQRILQAGWWTGGGFLLDKVIAAVQ
                     LAVLVRILTPADFGLMAASAAVLLAMLTISELGLDSALIAKEIVSEDDLAAAWTLSVA
                     RGLLMATGIWIMAGVIGEIMRMPTLESLLRVHAWALIIQGLQSPALALMMKKLDLRRR
                     VALDVVRRIVEAVVTLTIAILYRTVWALVIGQLVGLMIGSLLSFRIAPFTPGRSLKPS
                     AFSYFVRYGRHLNLTTLCAFGVMTGGELVIGRLLGPEALGLYQVALAIPLLIGARATA
                     LMQQISVPTYASLQGDQRGLVRVFDLQMGLVGIVYIPLAVTIGSLAPSMVPTVFGRQW
                     TGISDSLQVFCIYSVCAGYASVMASLHYGLGRPDLQVKSWVAQCALYVTMIVPMTVSL
                     GVFGAAIALTASYMFGTTLQIIETRRLLGTSTDETFVSLARTGLLGLAAGALLWIGVG
                     QTRLAIPWTPEVLAMATAAIFSWYLWRVERPRLKTLWNYQSQGGT"
     gene            complement(2000..>2329)
                     /locus_tag="VN647_02650"
     CDS             complement(2000..>2329)
                     /locus_tag="VN647_02650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG95960.1"
                     /translation="VVLHRQTRSYPGDSMKLWEYLACGRPVVATVGPGYGDTVEALRC
                     GLSAKADDPTDFARQLQCLFVDQDLRRSMGERGRMAVVRSHTWAARAAQLDRVCGQAI
                     AEMKSAA"
BASE COUNT          560 a          708 c          622 g          439 t
ORIGIN      
        1 ccttgccgcc aacgcctcca gccatgcaga agcgaatttt cgaagaaagg atgcgtggat
       61 gagatggtcc tggatcgaag ggaacgggaa acaggactgg tatagccggc cgttttccgt
      121 ccattgcttg caccctacgg cgccgatcct cggatctcca tcgagacatg ccaccatccg
      181 atccaatgcc tgccgtcgta ccagcgtatc gctgttcaac atgaggagat atcgtccagc
      241 actaaccgcc aacgcttgat tgacgccccg tgcgaaaccc cggttctcgc gattcacgat
      301 gacgcgcacc gtgggatagt ttgttcgaac ggcttccacc gtcccgtctt tggagtcgtt
      361 atcgacgacg atcacttcaa atcgggtcgc gagagtggac tcgaacaacg atgttaggca
      421 atcaagcagg agcactctgg tgttataact cacgatcacg atggatagat ccacggagcc
      481 gtcggacatt acgtccctcc ctgcgattga tagttccata gtgtcttgag tcgcggccgc
      541 tcgacccgcc agagatacca actgaatatt gcagccgtcg ccatggctag gacttccggt
      601 gtccacggga tggcgagccg agtctgaccg actccgatcc acagcagtgc gccggccgcc
      661 aggccaagga gtccggttcg agcaagcgat acaaacgtct cgtcggtcga agtgccgaga
      721 agccgtcgtg tttctattat ttgcagtgtt gtgccgaaca tatagcttgc cgtcagcgcg
      781 atcgcggcgc cgaacactcc gagcgagacc gtcatgggaa cgatcatcgt cacgtacaga
      841 gcacattgtg cgacccaact ttttacctga agatccggtc gtcccaaacc atagtgcaag
      901 gaggccatga cactggcgta gccggcacag acagaataga tgcaaaagac ttgcagcgag
      961 tcgctgatgc cggtccattg ccgaccgaac acagtcggga ccatgctggg cgccaaagaa
     1021 ccgatggtga ccgcaagggg aatatagacg atgccgacaa gccccatctg cagatcgaag
     1081 acacgaacca ggccgcgttg atccccctgc aatgaagcat aggtcggcac gctgatttgc
     1141 tgcatgagtg ctgtcgcacg ggcaccgatc agaagtggaa tggccagcgc cacctgatac
     1201 aatcccaacg cctccggtcc tagcagacga ccgatcacca gctctccccc ggtcatgacg
     1261 ccgaaggcgc acagggtggt caggttcaaa tgtctgccgt accggacaaa ataggagaat
     1321 gccgatggct taagggaccg acccggcgta aatggggcaa ttcggaaaga aagcagactc
     1381 ccgatcatca agccgaccaa ctgtccgatg accagcgccc acacggtccg atagaggatg
     1441 gcgatggtaa gagtcacgac tgcctcaacg atgcgccgga cgacatcgag tgcgactctt
     1501 cggcgcaagt ccagcttctt catcatgagt gccaacgccg ggctttggag cccctggatg
     1561 atgagggccc atgcgtggac tcgcaagaga gactccagcg tcggcatgcg catgatctca
     1621 ccgatcacac ctgccataat ccagatccct gtggccatca aaaggcctct ggcgacggac
     1681 agtgtccagg ccgctgcgag gtcgtcttcg ctgacaatct ccttggcaat gagtgccgag
     1741 tctaacccca gctcactgat cgtgagcatc gccagcaata cggcggcaga agccgccatc
     1801 agaccgaaat cggccggcgt cagaattcgt accaataccg ccaattgaac ggccgcaatc
     1861 actttgtcaa gcaggaatcc tcctccggtc caccaaccgg cctggagaat cctctggcgt
     1921 aaacgcaacg gactcgattc ggcgtcctgg atgctcatcg acgacaagtc agtcatggtc
     1981 ttttcacatc gtgattcgtt taagccgccg atttcatttc cgcaatcgct tgaccacaaa
     2041 cccgatcaag ttgcgcagca cgcgccgccc aagtatgaga tctgaccacg gccatccgtc
     2101 cccgttcacc catacttcta cgaagatcct ggtcgacgaa aagacactgg agttgacggg
     2161 caaaatcagt gggatcatcc gctttggccg acaaaccaca gcgcaacgct tcaacggtgt
     2221 ccccatatcc agggccgact gtcgcaacaa caggacgccc gcaagccaag tattcccaca
     2281 gcttcattga atctcccgga taggaacgag tctgtcgatg cagcacgac
//
LOCUS       DATNDD010000110         2843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_653911, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000110 DATNDD010000000
VERSION     DATNDD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653911"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..555
                     /locus_tag="VN647_02655"
     CDS             352..555
                     /locus_tag="VN647_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWG95961.1"
                     /translation="MNVSVRTAYNQWTQFEEFPQFMEGVEQVRQIDDKHLHWKASIGG
                     KQQEWDAEITEQIPDKRIAWRSQ"
     gene            888..1178
                     /locus_tag="VN647_02660"
     CDS             888..1178
                     /locus_tag="VN647_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95962.1"
                     /translation="MKARILSMLVVGVSGSGLGMAYSADPIAEKEANPTIEERLTQDT
                     VTGTVTRKEGTLMRKEGEYYYIRDNGGVVQRIHVDKSTKLDKVITGAWLKPM"
     gene            complement(1308..1910)
                     /locus_tag="VN647_02665"
     CDS             complement(1308..1910)
                     /locus_tag="VN647_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWG95963.1"
                     /translation="MSAGGLHGMYDIPSGFHLCLFYRSPKEFLPFWNMRLYQPALYIL
                     IVFVSACGTHIIHQPTLNLDRESQFTNVTVHDCSEADTCTISLSDPTLPPIFGQNIPI
                     RLKGIHAWAIKGRCPQETELAKKARDFLRAQLGKAMRIDVVEPERDKYFRLHGYLIMD
                     GNNLSTALIAAGHAQPDSGGTKLHPPCPAHTPLNCKLQCR"
     gene            complement(1956..2516)
                     /locus_tag="VN647_02670"
     CDS             complement(1956..2516)
                     /locus_tag="VN647_02670"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HWG95964.1"
                     /translation="MNTIKSCGVLLGTIMLSGCITEATLELTKAPFDATTALTNGTSK
                     AVGELLEPTQKFLSSTTPGTAAVERFTRAREKTELFVGYSSEHLRAESARGSGEHLAS
                     LAVLAGIPADRRLQFQEHMRETYSTMFDESLLPREVRLRIVNAAWSEGYGRLESETIK
                     HQETGISDIDLKIGKSRHSTAFMSVR"
BASE COUNT          710 a          708 c          779 g          646 t
ORIGIN      
        1 gccatcgcca cagcgatgcc gaacgcgccg gtggtaccga gcaaccgaga gcaactattt
       61 gccgatcggt taaacatact cggctgtttg ccaagggatg aaggattttc catcacttat
      121 catcgttaca gggggatgcg gcacgtacta ggccatgccc ttgattactc ttgtttcgcc
      181 tttacacgtt cacccctttt tctctgccat cctccagcct gtgtgctttc ctagtacgac
      241 ggtaggggcg aaagggacta cattgactaa taattccttt ttagcattac ctgacctcat
      301 accaatcgaa aggaggtgcc acgatgtctg tcataagaaa aatccattga cctgaacgtt
      361 tcggtacgta ctgcctacaa tcaatggacc cagttcgaag agtttcctca gttcatggag
      421 ggggtcgagc aagtacggca gattgacgac aagcatctcc attggaaagc cagcatcggc
      481 ggaaagcagc aagaatggga tgcggagatc accgagcaga ttcctgataa acggattgcc
      541 tggcggagtc aataaggggc gaagaatgaa gggatcgtca tattttctcc tgtcacagag
      601 ggaaagtcga agatcaacct tcgcatcgac tatgagccac aaggggtaat cgcaaaaacg
      661 ggtgatgccg tgggaatgat ttctcaacga gttgaagggg acctgaagcg attcaaggaa
      721 tacatcgagt cgcgcgggca agaaaccggc gcctggcgag ggagcgtaag ctgagcgaac
      781 acggacaccc ctcccaggat ttggctaaag ttgtactgta acggatgtgt tctgatccat
      841 tcctgatgac cgaaggaagt gggggaatac tgaacggagg acaccaattg aaagctcgaa
      901 ttctatcgat gcttgtggtc ggagtatcgg gttccggcct cggcatggca tactcggccg
      961 atcccatcgc tgagaaagaa gcgaacccca ccatcgaaga gcgtctcacg caagataccg
     1021 tcacgggtac cgtaacgaga aaagagggta ccttaatgag aaaagagggt gaatattatt
     1081 acattaggga caacggtgga gtggtccaaa gaatccatgt cgacaagagt accaagttgg
     1141 ataaagtgat tacgggcgct tggttaaagc ctatgtgaca gaccagggcc atacaacgac
     1201 attgcagcgg gataattgag accggccgtc actgtctttg tttgaggagg gaatgccgat
     1261 agccgatatc tccagctgac tcaccaaccg ggcggtgacc ggcccgttta cctacattgc
     1321 agtttgcagt tgagcggggt atgcgccggg cacggggggt gaagcttggt tcccccgcta
     1381 tcgggctgcg catgaccagc ggcaatgaga gcggtggaaa ggttgttgcc gtccatgatc
     1441 agatagccgt gcaggcgaaa atatttatcc cgttcgggct caaccacatc gatgcgcatg
     1501 gctttaccca attgggctcg aagaaaatca cgtgcttttt tcgcaagctc cgtttcctgg
     1561 ggacaccggc ctttgatcgc ccatgcgtgg attcccttca gacgaatcgg gatgttctga
     1621 ccaaatatgg ggggcagcgt tggatcgctc aacgagatgg tacatgtgtc ggcctcggag
     1681 caatcatgga ccgtcacatt ggtgaactgt gactcgcggt caaggttcaa ggtcggctga
     1741 tgaatgatat gagtgccaca ggcggaaaca aatactatca gtatataaag ggcgggctga
     1801 tacagtcgca tattccagaa ggggaggaat tccttgggac tccgatagaa tagacagaga
     1861 tggaaccctg acggaatgtc atacatgcca tgaaggccac cggcagacat cgcgacatcc
     1921 tccatacgtc atgccactcc ccttcacaga cgggctcatc gcacagacat gaacgccgtc
     1981 gagtgccgag acttgccgat cttcaagtca atatcagaaa tccccgtctc ctggtgttta
     2041 attgtctcgg attcgagtct gccatatcct tccgaccacg cggcattcac aatgcgcaac
     2101 ctcacttcac gaggcaggag ggattcgtca aacatcgtcg aataggtctc gcgcatgtgc
     2161 tcctgaaatt gaagacggcg atccgcagga atgccggcaa ggacagcgag agaagccagg
     2221 tgttctcctg atcctcgtgc gctttcggcc cgcaagtgct cagaggaata gcccacaaag
     2281 agctccgttt tctccctggc acgcgtgaag cgctcaaccg cggctgttcc cggcgtcgtg
     2341 ctggatagga acttttgcgt gggctcaagc agttcgccta cggctttcga tgtgccattg
     2401 gtcaacgccg tggtcgcatc gaatggagct ttcgtcagtt ccagcgtcgc ttccgtgatg
     2461 cagccagaca acattatcgt tccaagcagt acgccacacg atttgatagt gttcatatta
     2521 tactccctaa ttatttttca tatagttatt ctcttgaatc agtgtagcaa accatattta
     2581 tttgtggaac ctaggagaaa ccataggtgt gggccgttcg ttgagccggc cagtcccacc
     2641 gagagcctct ttgcttgggg gctttccgag tcgtgaacgg cgctctcgca aaaaacagag
     2701 tgatccgaca ctgcgaggct atgtggcagt gtttaattcg ataggtcatg atctgggcag
     2761 atgcaaggag agatccttcc cagcgcctgt actgattcca tcaagaggcg cgaagtgctg
     2821 atgctctatg gacatgatca tct
//
LOCUS       DATNDD010000111         3305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_954090, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000111 DATNDD010000000
VERSION     DATNDD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954090"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VN647_02675"
     CDS             <1..387
                     /locus_tag="VN647_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95965.1"
                     /translation="RVTVSIAVVASATPPAPSGSPPPTPEELARQQQMLAAQAAQQQE
                     QLRSQQLQEQMRQYRYLGYVNQNGVQKAFLGKGREIHIIREGEVLDGKFVVVSIDATT
                     VKITDSDSKLEITLKLKEEEGNHSGI"
     gene            complement(470..1414)
                     /gene="gspK"
                     /locus_tag="VN647_02680"
     CDS             complement(470..1414)
                     /gene="gspK"
                     /locus_tag="VN647_02680"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HWG95966.1"
                     /translation="MPRADERGIALLMALLVLTILTALVLEFDAEARREYRAAAAFRD
                     DYKAGMLTRAAVQAARAVLQQDLFREKMTGQKYDGPTDIWAMPIKNYAIGDGFLTAQI
                     QDETGKFNLNDLASTSGGDIAQKKKVLRAKRLFELLRVNPNLVDALIDWIDQDEVPQP
                     AGAESLYYQSLRPPYQSANGPLPGLGDLRLIKGFTPEIIERITPYVTVFPQEGGAMMN
                     VNTADPIVLQTLDPSVTQSVALEIVQGRPYKTKVELDRVGSFQEIGKTLRSDYDIKSD
                     YFSARLAVTVNETTKTAWALLKRDAGKGESTVEYMRIL"
     gene            complement(1401..1949)
                     /gene="gspM"
                     /locus_tag="VN647_02685"
     CDS             complement(1401..1949)
                     /gene="gspM"
                     /locus_tag="VN647_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HWG95967.1"
                     /translation="MTQSFRERWRQMSQRERTLVSAGGIVLGLSLFFVLVVDPLLDTL
                     DRLDRQEVRKQKDLAELAVLGQAYLAKRDRSALTESRMPGADSHFSLLTFMEEAATTA
                     HMRERITGMQPQVQTLAQGYEETAVDLRLEGIQLPDLLALLVAIDQAPYDLYVRHLQI
                     RPKFDNPINLDATVRVLSYAKS"
     gene            complement(1946..>3305)
                     /gene="pilM"
                     /locus_tag="VN647_02690"
     CDS             complement(1946..>3305)
                     /gene="pilM"
                     /locus_tag="VN647_02690"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HWG95968.1"
                     /translation="PCQDLFVRTLSFPFKDSTKLAQVVPFEVENLVPMPLEDLAVGSL
                     VLPPGQTAEGTVHESKGSEVLVTAAPRDKVAEHLRFLAQADVEPSAINVDAMALFSVT
                     QYLQEEGAAVPQDLAIIDVGASKTTLCLVQGGRPMVLRTILWGGNHLTHALAVRHVCS
                     FADAERRKRTMAVNQVDGLLEPILKELRITLQAHQGNERSRLTHCWVCGGGAKLQEIG
                     GYMARQLGLYPVGPRQGFGAEAPRAFSIAFGLAIHPKIIRPRWRFKSTPMELALDLKD
                     VAEAASPDTAATKQDRRLAIAAGLIIAVLAIVDFSIHFMLHDQRVARLKTTLHSRYEQ
                     VFGPGAAPGEELDQAQYRLGVLDKALAVTDTSDGNVLRTLSAFVKQLPPGTMVKVREL
                     TVDGATILMEGETTSFDAVERLKQTFASSPQLRDVAVTETRVGAGPNQVVFRITVTVE
                     KP"
BASE COUNT          671 a          953 c          966 g          715 t
ORIGIN      
        1 cgggtcaccg tcagcattgc tgttgtcgcc agtgctactc caccagctcc ttctgggtcg
       61 ccgcctccga caccggagga attggcgcga caacagcaaa tgctcgcggc ccaagcggcc
      121 cagcaacaag aacagctgcg aagccaacaa ctccaagagc aaatgaggca atatcgttat
      181 ctggggtatg tcaatcagaa cggcgtgcag aaggcctttc tcggcaaagg ccgcgagatt
      241 catattattc gtgaaggaga ggttttggat ggaaaatttg tggtggtgtc aattgacgcc
      301 acaacggtga agattacgga ttccgactcg aagctcgaaa ttacccttaa gctgaaagaa
      361 gaggaaggga atcattctgg gatttgatcg tcagatagaa caggtatgat ttgctctcgc
      421 cccttagcga gcggtatcat tcaaggcaat agatgcccgc tccatctact cacaagatcc
      481 gcatatattc gacggtgctc tcccccttgc cggcgtctcg cttcagtaag gcccatgcgg
      541 tcttggtcgt ttcattcacc gtgacggcga ggcgcgcgga gaaatagtct gatttgatat
      601 cgtagtcgct tctcagtgtc ttgccgatct cctgaaagct cccgactcga tcgagttcca
      661 ctttcgtctt ataggggcgt ccttggacga tctccagcgc gacggattgg gtgacgctcg
      721 gatccagcgt ctgaaggacg atgggatcgg ccgtgttgac gttcatcatc gctcctccct
      781 cctgcgggaa gaccgtcacg tagggagtga tccgctcgat gatctccggc gtgaatcctt
      841 tgatgagccg gagatctccc aaccccggta acggaccatt cgcagactga tagggcggcc
      901 tcaacgattg gtagtagaga ctctcggcac cggccggctg gggcacttcg tcctgatcaa
      961 tccaatcgat gagggcatcg accaggttgg ggttgaccct gagcagttcg aataaccgct
     1021 tggctcggag gaccttcttc ttctgcgcga tgtcgcctcc cgaagtggag gcgaggtcgt
     1081 tcaagttgaa tttccccgtc tcatcctgga tctgcgcggt cagaaatccg tccccgatcg
     1141 cataattctt gatcggcatg gcccagatat cggtcggacc atcgtatttc tgccccgtca
     1201 tcttttcacg aaagagatct tgctgcagca cggctcgcgc agcctgcacg gcggctcggg
     1261 tcagcatgcc tgccttataa tcgtcccgaa aggcggcggc ggctcgatat tcccggcgcg
     1321 cttccgcgtc aaactcaagg acaagggcgg tgaggatcgt cagcaccaac agcgccatga
     1381 gcagcgcgat gcctcgttcg tcagctcttg gcatagctca agacccgcac ggttgcatcg
     1441 agattgatcg gattatcgaa tttcggtcgt atttgcagat ggcggacgta aagatcgtaa
     1501 ggggcttgat cgatggcgac gagtaatgcc aataagtcgg gcaattggat gccttccaat
     1561 cggagatcga cggctgtttc ctcgtagcct tgcgccagcg tttgtacttg cggttgcatg
     1621 ccggtgatgc gttcacgcat atgggcggtg gtggccgctt cctccatgaa cgtcagtagg
     1681 gagaaatgac tgtcggctcc cggcatccgg ctttcggtca aggccgagcg gtcccgtttg
     1741 gcgagatagg cttggccgag caccgccaat tcagccaggt ctttttgctt ccgtacctcc
     1801 tggcgatcca gacgatccag tgtgtccagc aaagggtcga cgacgagaac gaagaagagg
     1861 ctgagcccaa gcacaatccc gcctgccgac acgagagtgc gttcacgctg cgacatctga
     1921 cgccagcgtt ctcggaaact ctgcgtcatg gcttctccac cgtgacggtg attcgaaata
     1981 cgacttgatt gggacctgcg cccactctgg tttccgtcac cgcgacatcc ctcaattgag
     2041 ggcttgatgc gaaggtttgc ttgagtcgtt ccacggcgtc gaaggaggtg gtctcaccct
     2101 ccatgaggat ggtcgcacca tccacggtca actcgcgcac cttgaccatc gtcccaggcg
     2161 ggagttgctt cacgaacgcc gagagcgtac gcaacacgtt gccgtccgag gtatcggtga
     2221 ccgcgagcgc cttgtccagg actccgaggc ggtactgggc ttgatcgagc tcttcgcccg
     2281 gagcggcgcc cggtccaaaa acttgctcat accggctatg aagcgtagtt tttaaccgag
     2341 cgacccgctg gtcgtgcagc atgaagtgaa tcgagaaatc cacgatcgcc agaacggcaa
     2401 tgatcagtcc ggcggcaatt gccaggcggc ggtcctgttt ggtcgcggcg gtatccggcg
     2461 aggcggcctc ggcaacgtct ttgagatcga gcgcgagctc catgggagtc gacttgaatc
     2521 gccatcgcgg acggatgatc ttcggatgga tcgccaagcc gaacgcaatg gaaaatgccc
     2581 gtggggcttc ggctccgaac ccctgtcgcg gtccgaccgg atagagtccc aattgtcggg
     2641 ccatatagcc gccgatctct tgtagcttgg ctccccctcc gcatacccaa cagtgcgtca
     2701 agcggctgcg ttcgtttccc tgatgggcct gcaaggtgat gcgaagctct ttcaggatcg
     2761 gctccaacag accgtccact tgattcacgg ccatcgtccg ttttcgacgt tctgcgtcgg
     2821 cgaaactaca gacgtgccgg acggccaggg catgtgtcag atggttgccg ccccacagaa
     2881 tcgtccggag gaccatgggg cgtcctccct gcacgaggca gagcgtcgtc ttcgaggctc
     2941 cgacgtcgat gatggccagg tcttgcggga cggccgctcc ttcttcttgt agatattgag
     3001 tgacggagaa gagggccatc gcgtcgacat tgatcgccga gggctccaca tcggcttggg
     3061 ccaggaaccg gagatgctcc gcgaccttgt ctctcggggc ggccgtgacc aagacctccg
     3121 aacccttgct ctcgtggact gtcccttccg ccgtctgtcc cggcggcaac accaggcttc
     3181 cgaccgcaag gtcttcgagc ggcatgggaa cgagattctc gacctcaaag gggacgactt
     3241 gagccagctt cgtcgaatcc ttgaagggaa aggacaaagt tctgacgaaa agatcctgac
     3301 aagga
//
LOCUS       DATNDD010000112         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_525350, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000112 DATNDD010000000
VERSION     DATNDD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525350"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /gene="folK"
                     /locus_tag="VN647_02695"
     CDS             <1..449
                     /gene="folK"
                     /locus_tag="VN647_02695"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWG95969.1"
                     /translation="GVSLLYETEPVRDRTDPGEGWFLNGVVQLETDITPWSLLTTIRE
                     IERSLGRDDDNRSGPRTIDLDILFYGEHVIKEPGLTIPHPRLHQRRFVLMPMNELDPL
                     WVHPTLKQSVAQLLTGVKDQSQVRFLFPQPSTRYGSRPACGSPSGS"
     gene            446..1312
                     /gene="panC"
                     /locus_tag="VN647_02700"
     CDS             446..1312
                     /gene="panC"
                     /locus_tag="VN647_02700"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWG95970.1"
                     /translation="MKIIRSLTAMTAWSEQLRREGVRIGLVPTMGALHEGHRALIRAA
                     RLRCDALVVSIFVNPTQFGPNEDLAKYPRPLSRDRALCEKEGIDVCFEPTVEAMYPSR
                     FQTTVALPAIARRCEGEVRPHHFSGVATVVTKLFGIVRPRIALFGQKDFQQSALVRQL
                     VKDLDLGVEIVVHPTVREADGLARSSRNIYLLPDDRIRAATLYKSLQAGGEAIRRGVT
                     DGATIQSEMAQVLKKESAITIDYLAVCDPATLEPLLSVTRQAVLLGAIRLGSVRLIDN
                     ILVSAKQARHKL"
     gene            complement(2058..2423)
                     /locus_tag="VN647_02705"
     CDS             complement(2058..2423)
                     /locus_tag="VN647_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWG95971.1"
                     /translation="MKIGMTPSHPGSFIKTEILDELELSITKAAEILGVRRATFSDLV
                     HEKAALSPLALRLEKAFHLKMEMLLQMKAWYDSNEMRKLTRKELTVRKQGKSSIPYIH
                     LAASTIQKRTAGIRTTTEL"
     gene            complement(2669..>2886)
                     /locus_tag="VN647_02710"
     CDS             complement(2669..>2886)
                     /locus_tag="VN647_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95972.1"
                     /translation="ICSSQSSFIELVINRLPTKSLLRSRRPWSVLLGPESMGIRFLEM
                     VPDDKNRLSQVILSLLVGQSANLQLFS"
BASE COUNT          647 a          759 c          806 g          674 t
ORIGIN      
        1 aaggcgtttc actgctgtat gaaacagaac cggttcgaga ccggaccgat cccggagagg
       61 gctggttctt gaatggggtg gtgcagcttg aaacggacat cacgccatgg agtctcttaa
      121 cgaccatccg agaaatcgag cgctctctcg gccgggatga tgacaaccgg tccggtcctc
      181 gtacgatcga cttagacatc ctcttttatg gcgaacatgt gatcaaggaa ccagggttga
      241 ccattccgca tcctcgtctc catcagcgac gatttgtgct catgccgatg aatgagttgg
      301 atccgctgtg ggtccatccg acgctcaaac agtctgtggc acagttattg acaggagtca
      361 aggaccagtc tcaggtgcgt ttccttttcc cccagccttc tacacggtat ggctcgcgtc
      421 ctgcctgcgg ctcgccgtcc ggttcatgaa gatcattcgt tcacttacgg ccatgacagc
      481 ctggagcgag caactcagac gtgagggtgt gaggattgga ctcgttccga caatgggggc
      541 gctgcacgaa ggtcatcggg cgttgatacg ggcggcgcga ttgcgatgcg atgcgctcgt
      601 cgtcagtatc ttcgtgaatc ccacgcagtt cggtcccaat gaagatctgg ccaagtaccc
      661 acgccctctc tcgcgggatc gagctttatg cgaaaaggag ggcatcgatg tctgttttga
      721 gccgaccgta gaggcgatgt acccgagcag atttcaaacc acggtggcac ttccggcgat
      781 tgcacgtcgg tgtgaagggg aggtgcgtcc gcatcatttt tccggcgtgg cgaccgtcgt
      841 gacgaaactt ttcgggatcg tccgtcctcg gatcgcgctc tttgggcaaa aagattttca
      901 acaatcggca ctcgtccggc agctggtgaa ggatctggac ctcggtgtgg agattgtcgt
      961 gcatcccacg gtgcgcgaag cggacggact cgcgaggagc tcacgcaata tctacctatt
     1021 gcctgatgac cgaattcgtg ccgcgacgtt gtacaagagc ctgcaagcgg gaggcgaggc
     1081 gattcgaaga ggcgtcaccg acggggcaac aatacagtca gaaatggctc aggttctcaa
     1141 gaaagaatcg gccatcacga tcgactatct ggccgtctgc gatccggcaa ccctcgaacc
     1201 actcttatca gttactcggc aagcggtgtt gcttggagcg attcgtctcg gatctgttcg
     1261 gctcattgat aatattttgg tttccgccaa acaggcgcgg cataaactgt gagtaaggtt
     1321 ggccttagaa ttcactctcg cttctcgagt ctgcatcgag caattgggaa tccaataaca
     1381 acgcatcagc tcttagttgt ggccgcggat ggcacacacg caactcaact gacgattcct
     1441 acggagacag gtgatatcac cccggcgtgg tcaccagatg gcgatcggat tgccttttcg
     1501 agtaaaggaa gtatcgtcgt agcgaagatc caggcgatca acggtgtacg aagcctcgat
     1561 ccggctgtca aagtctccac gcctcctgcg ggatcgatag atcaacatcc cacatggtct
     1621 ccagatgggc gctggattgc atttgaacag tatcccttgt ctcagctgga tcagaatggg
     1681 aattccctaa cggggcaact gtggattgtc gctccaggcg gaacgggatt gacgcaactg
     1741 acaccaggga cagtgacgcc aggaacacag gttcttccct caggaaagcc aacatggtct
     1801 ccgtgttttg cgcctaaaac tccgtgagga gtgtacgaaa atgaagggct tgtacatcgc
     1861 gaaggttcca gtcaacaagt tagcatgcat cccatactct gatgagcgcc tcaaaaacat
     1921 aagtgaccta gactagagga ggtcagttct tgaatcgtgg catgaccaag gaatggttgt
     1981 tcggtcttgc ctcagtcacc cccgcagacg ccacggccac agatccagca tttagggtgg
     2041 ggtcgcttct tgcaatacta caattcggtt gtagttctga tgcccgcggt tcgcttttga
     2101 atggtggagg ccgctagatg gatgtaaggg atgctggact tcccttgttt ccgtacagtc
     2161 agttccttcc gggtcagctt gcgcatctcg ttgctgtcgt accaggcctt catctggagc
     2221 agcatctcca tcttcagatg aaaggctttt tccagtcgca acgccagagg ggagagcgcc
     2281 gctttttcat gcaccaagtc ggaaaacgtg gcccggcgca cgcccaagat ttcagcagcc
     2341 ttggtgatgc tcagctctag ctcgtctagg atttccgttt tgatgaagct gcccggatgg
     2401 gaaggcgtca tgccgatttt catgcttttt cgggccatta gtgatagtcc tccaaatcta
     2461 agccatccac atacccattc tcctccgcaa acgtgatccg ccaggaagtt ttaacaccga
     2521 tccagacgga ctggctatga ggagttctct cagtcgatcc ttcaggatgt caggggtctt
     2581 gcgataggct cgaatccaga tggtggtgtc gatgaggcga ggcctaagat caggagcggc
     2641 atgtgcgatg gcataccgga gagatccatc acgagaaaag ctgtagattg gcgctttgtc
     2701 ccaccagcaa gctgagaatc acctgactga ggcgattttt gtcatccggc accatttcga
     2761 ggaaccgaat gcccattgat tccgggccca gaagaacaga ccatggccgt ctcgatctta
     2821 ataggctctt cgtcggaagc cgattgatga ccagttcgat aaacgagctt tgtgatgagc
     2881 aaatgt
//
LOCUS       DATNDD010000113         9058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2952918, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000113 DATNDD010000000
VERSION     DATNDD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2952918"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VN647_02715"
     CDS             complement(<1..1564)
                     /locus_tag="VN647_02715"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95973.1"
                     /translation="MGRKTKTTAPDGLVTTVTYPTLAEFGVIGTQKISTTTSGASLPA
                     NLVSSTFFDGLGRTFKKESSGPNSTTLVTETQYDVKGQMFQTSLPYFKTTESVTGRWR
                     TMTYDPVGRVTQITHPDTLSGTALTSQSCYAPFVTVTLDPSGARKRETKDAYGRVVKI
                     EEYSTTTSTCDTAVGTVYASTNYTYDLLGNLTKVVDALGNRTTMRYDSLSRKIAMSDP
                     DMSTNGTSTCGDLTTLSPAGTYPWYATPCWNYQYDAAGNLTRQTDAKNQTLWFRYDGL
                     NRRSQKDFTTQKAQGSGDVVYVYDDTVTTFNRKGRLKQVTDVATNVTFEYDAMGRISK
                     STKMLDGTTYVTTSVYDGLGRLKEVTYPTSPAKTVEYLYTGPVLDKVQDKAGSGTTIY
                     ATYSNYTSQGQAQTITYGNGVVTTSTFADPAHPTCIPANNFKLCTLKTQKGTNPLYQD
                     FTYTFTADGNVDLISDPINGNQDFGYDALDRLTSATGPYGASGATTTFSYTYNQIGNM
                     LSNPQVGTYTYPT"
     gene            1787..2995
                     /locus_tag="VN647_02720"
     CDS             1787..2995
                     /locus_tag="VN647_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HWG95974.1"
                     /translation="MPPLNFEAPAGFAVVLVAVASAIAGFVNYSPDERVRAIVGTVVA
                     FFMNMLVLLASISPWLLSNMNSLATRHLLPWYLFYCASAVGVLYTVLSRLLKMKTTWI
                     PVAVLLGVLVPIAVAQYRLSSLEVMVSSLEIQTMRDRISDWVETKGWMNNRYVLVILP
                     TRPRPTVVETLYPNIGSGDINAVLASSQNTVSIPWMLNALLRERHDRPQLLLIDCSSD
                     QACANAAAQDPKVIVLGYTNDVTVISSAVAPFIINLSLLTSSPVAPSIVITKGPTVTA
                     SSMFKNLGPYGLLTAMKPGWHSEPHPNYPQIIEMDLADEKAFDKLGLLPQANFVSRMP
                     SRVEVSVRSENGSWEEKGVFDDLCNTTGADGWHTLRLDSPVRARFLRLNILRNCGDPE
                     LLTLRGLRFE"
     gene            complement(3248..4039)
                     /locus_tag="VN647_02725"
     CDS             complement(3248..4039)
                     /locus_tag="VN647_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWG95975.1"
                     /translation="MSDPREPIIRFDHASFGFPGLIALQDISLTIYEGEFVGVIGPNG
                     SGKTTLCRAVLGLLAPVEGHLHIFDCACDELRCHHRAKIGYLPQKGVVDRNFPVTVLE
                     TVMMGRYGALGLFKRPGRKDHDIALEALAQVGMESHKDNALGHLSGGQQQRVFIARAL
                     AQQPKVLILDEPTTGLDITTQHNVIELVQHLHDELKLTVLLITHDINMIRSRVDRLVL
                     LKTRLFAAAPPAEVLKPEILHQVYGKDLVITEKDLVIVEDYHHHH"
     gene            complement(4078..5013)
                     /locus_tag="VN647_02730"
     CDS             complement(4078..5013)
                     /locus_tag="VN647_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWG95976.1"
                     /translation="MIPSPPMFPANHVRKSPVFSLLAVLAGFLLSFGTITEARDPIPV
                     VVTIPVLKDLAEQVGGPHVRVTSLLSGYENEHTYSPKPTDLVAVRKARLLLEIGMGLE
                     VWVSSLVKNAGDPSLRVITTSRGVALIRDDADSHEGEAGNPHIWLDPENVPIMLRHIT
                     QAFIEMDPRHAADYQANQTAYLRRLGQVQQELLERTQRLSDRRFIAHHPAWPYLAKRF
                     SFNIVGTIHRQSGTEPSAYHLQFLIEKIRKENIKVVVSEVQLSRRLPELLARETKARV
                     VVLTAMPGGLAGTETYLDMLRYDVLQLAGALEATS"
     gene            5494..6693
                     /locus_tag="VN647_02735"
     CDS             5494..6693
                     /locus_tag="VN647_02735"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWG95977.1"
                     /translation="MKVVGLMSGTSADGVDAALVTIARQRSDLRVGMLAFHSLPYPRS
                     LQQRILSASVSGTVTDICHLNALLGEWFADAALGVIRSAQLSPEDIDLIGSHGQTVHH
                     LPHGIKDTRVGAIRSTLQIAEPAVIAERTGITTVADFRPRDIAAGGQGAPLTPGSHAL
                     LFRHPRRARLIVNLGGISNVTYLPPGSGSEDLVAFDTGPANMVLDGLMSRTTNGRSSM
                     DREGRTATKGRVDSRLLAKLLAHPYLSQAPPKSTGREAFGEKMLDELLNWQQTRRLSV
                     EDLLATCSRWTAEAVGTARRWIRGGIDDVIVGGGGVRNRAIMGHLTEIFAPVPVTSFE
                     SHGWDSKALESVAFAVLAYQTVMEQCGNVPSVTGAASPRLLGCIVPSGPRWYERLRSR
                     KGGSKTR"
     gene            6690..7196
                     /locus_tag="VN647_02740"
     CDS             6690..7196
                     /locus_tag="VN647_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HWG95978.1"
                     /translation="MKILLAGAVIAVSIGLWGYLRRARRRKKQASPFAVPHDATISSV
                     RLLAHEEVALYSLLQMAVQERYLVFSQVPLWSFIEVEAGEKMRADILRRIALKRVDFV
                     LVHPGSCLVEQVVQIERESSQPHEAERQHVTESVLDAAGIKLTKLQPKESHSLPDLAA
                     RLGLVEEE"
     gene            complement(7179..7328)
                     /locus_tag="VN647_02745"
     CDS             complement(7179..7328)
                     /locus_tag="VN647_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95979.1"
                     /translation="MFEILARTTNVNVLFDKEVRDDPITIFIKDLSRPRVLLTEQPSS
                     LFLFD"
     gene            complement(7380..7895)
                     /locus_tag="VN647_02750"
     CDS             complement(7380..7895)
                     /locus_tag="VN647_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG95980.1"
                     /translation="MQYHRSFGIGTIVVCALAGCVSPDLRQAHRLAAEGQWDQAVAAY
                     QEAQRKAPFDEKIQRQLDQAKTAAAADHYALGKRAFENEQLAGALHEFKLALGLDPSK
                     AEYHAMIADALRLKESQEQLQTAKKLQNLGRVEEALTAFERAVELDPTQSKALDGITE
                     LTKQQRAAKTF"
     gene            complement(8398..8874)
                     /locus_tag="VN647_02755"
     CDS             complement(8398..8874)
                     /locus_tag="VN647_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95981.1"
                     /translation="MEHASHDDCPNVSGTYKLYGEALPGMPPHSRFSSGLALDVMLSL
                     DLNVPERWPTSDIQVVQTDTHTISVRIIGSSIIKTMQLQPRDKLWCKDQQLVIERLMD
                     TTGEATAGRALVIHRLELAQDGALIVRTEIRRGTRFLFFYIKDPTELYGARFEVVR"
BASE COUNT         1910 a         2442 c         2581 g         2125 t
ORIGIN      
        1 tcgtcggata ggtataggtg cccacctgcg gattactgag catgttaccg atctggttat
       61 aagtatagga aaacgtggtc gtcgccccgc tggctccata gggaccggtg gcgctggtca
      121 gccgatccaa ggcgtcgtag ccaaagtctt gattcccatt gatcggatcg gagatcagat
      181 ccacattgcc gtcggccgta aaggtataag tgaaatcttg atacagggga ttggtccctt
      241 tttgagtctt gagcgtacag agcttgaagt tattggcagg aatacaggtc ggatgggctg
      301 gatcggcaaa ggtgctggtg gtcacgacgc cgttgccgta ggtaatggtt tgggcttggc
      361 cctgtgacgt gtagttgctg taggtggcat agatcgtcgt gccactgccc gccttatctt
      421 ggactttgtc caagaccggc ccggtataga gatactcgac cgtcttggcg ggcgaggtcg
      481 gataggtcac ctctttgagg cggcctaaac cgtcatagac gctcgtggtc acataggtcg
      541 tgccgtcgag catcttcgtg ctcttgctga tccggcccat ggcatcgtat tcgaaggtga
      601 cattcgttgc gacatcggtg acttgcttga gtcggccttt ccgattaaac gtcgtgacgg
      661 tatcgtcata gacataaacg acatcgccgc tgccttgcgc cttttgagtg gtgaagtctt
      721 tttggctgcg tcgattcaac ccgtcatacc ggaaccacag tgtttgattc ttggcatcag
      781 tctgtcgagt gagatttccc gccgcatcgt attgatagtt ccaacaaggc gtcgcatacc
      841 acggataggt gccggccgga ctgagcgtgg tcagatcgcc acaggtactg gtcccgttgg
      901 tgctcatgtc aggatcggac atggcgatct tgcggctcaa gctgtcgtac cgcatggtgg
      961 tgcgattgcc cagagcatcg accactttgg tcaggttccc gagcaggtcg taggtatagt
     1021 tcgtcgaagc atagactgtt cccaccgccg tgtcgcaggt actcgtggtg gtactgtact
     1081 cctcgatctt caccactcgg ccataggcgt ccttcgtctc ccgcttgcgg gcgccgctgg
     1141 gatccaacgt cacggtcaca aagggcgcat agcaactctg actggtgagc gccgtcccgc
     1201 ttaacgtatc gggatgggtg atctgcgtca cgcggcccac gggatcatag gtcatcgtgc
     1261 gccagcggcc tgtaaccgat tcggtggtct tgaaatacgg gagactcgtc tggaacatct
     1321 ggcccttcac gtcatactgc gtctcggtga caagcgttgt gctgttgggc ccactgctct
     1381 ccttcttgaa cgtccggccc agtccatcga agaaggtgct gctcacgaga ttggccggca
     1441 ggctcgcccc gctcgtcgtc gtgctgatct tttgagtacc gatcacgccg aactcagcga
     1501 gggtgggata ggtgacggtg gtgaccagac catcgggggc tgtcgtcttg gtcttgcggc
     1561 ccatgtgtcc cgtcccttgc cggtgaggat tagagttctg ttttcaacgc tgtcatttta
     1621 tttcattacg tcaatctttt attacggcct cgtcaagctg agcgttttct ttgtggagat
     1681 gaggtacgga aaacttgaga gcgtaggcat ctatgacgta agcgtccaaa acactccttc
     1741 cgttgttctg gagcgcatac tggattgtct ccggtatttt tatgcgatgc ctcccctcaa
     1801 ttttgaagcg cctgccggct ttgccgtcgt ccttgtcgca gtagcttctg ccatagctgg
     1861 atttgtgaat tacagccctg atgaacgagt ccgcgccatc gtcggtactg tggtcgcctt
     1921 cttcatgaac atgctcgtct tgttggcatc gatttcgcca tggctgttga gcaacatgaa
     1981 tagcctggct acgaggcatc tactcccctg gtacctcttc tattgcgcat cagcagtcgg
     2041 tgttttatac accgtgttat cgcgcctcct aaaaatgaag acgacatgga tacccgttgc
     2101 agtgttgctc ggtgtcctgg tgccgatcgc cgtggcacag tatcgcctct cgtccctcga
     2161 ggtcatggtc tctagcttag agattcaaac aatgagggac agaatctctg attgggtgga
     2221 aactaaaggt tggatgaata acagatacgt acttgtgatt ctcccgacgc gtccacgccc
     2281 gaccgtggtt gagacactgt accccaacat aggctctggg gatattaatg ctgtgttggc
     2341 gtcctctcaa aataccgtgt ccatcccatg gatgctcaat gcgcttcttc gtgagcgtca
     2401 tgatagaccc caactcctct tgattgattg ctcctctgat caagcctgtg cgaatgccgc
     2461 ggcccaagat ccgaaagtaa ttgtgctagg ctatacgaat gatgtgaccg taattagcag
     2521 tgctgtcgcc ccctttatca tcaatttatc cttgctcact tcttctcccg tcgcaccttc
     2581 gatagtaatc acaaagggac cgacggtgac agcctcctcc atgtttaaaa atctcggtcc
     2641 ttatggccta ctcacggcaa tgaaacccgg ttggcattct gaaccacatc ccaattatcc
     2701 acaaattatt gaaatggacc ttgctgatga aaaggctttt gataagttgg gcctcctgcc
     2761 acaggcaaat tttgtatcgc gcatgcccag tcgcgtggaa gtaagcgtgc gatcagagaa
     2821 cggatcctgg gaggagaaag gtgtgttcga tgacttgtgt aatacgacag gagcagacgg
     2881 gtggcatacg ttaagactag atagcccggt aagggctcgt tttctaaggc tgaatatcct
     2941 tcgaaattgt ggcgacccgg agcttcttac gctaaggggg ctgcgcttcg aatagatatg
     3001 aatagggctt cgttttagat gcccaaaata tctggtcgaa ctgatctctg atcagaaccg
     3061 gtggcaactc aaaagtagcg tacgatagcg gctttgttgg tcctcgacga aaggagtgga
     3121 tcgattaagc ggtcgtctgc tccgcgtagg agttgtctcg aaacgaggcc aacgtcaaaa
     3181 gccaagaaat tgtcctttcg actggtctca ttgaagggag gagcttacgt ttcagaatct
     3241 gcaactttta gtgatgatgg tgataatcct cgacgatgac gagatccttc tccgtaatca
     3301 cgaggtcttt accgtacacc tggtgaagaa tctcaggttt gagaacttcc gcgggggggg
     3361 cggcggcgaa gagtctggtt ttgagaagga ccaatcgatc cacccgtgag cggatcatat
     3421 taatgtcgtg cgtgataagg aggaccgtga gtttgagctc gtcatgaagg tgctgtacca
     3481 actcgatgac attgtgttgg gtggtgatat ccagaccggt cgtcggttca tccaatatca
     3541 ggaccttggg ttgctgggcc agtgccctgg cgatgaagac gcgctgttgc tggccgccgg
     3601 acagatgccc gagggcgtta tctttgtgag attccattcc gacctgagcc aaagcttcca
     3661 gcgcgatgtc atgatccttc ctccctggac gtttgaacag gcccagcgct ccgtagcgac
     3721 ccatcatcac cgtttcgagg accgtcaccg gaaaatttcg atcaacgacc cctttctgcg
     3781 gaaggtagcc gatttttgcg cgatggtggc agcggagctc atcacaggca cagtcgaaga
     3841 tatgaagatg gccctccact ggagcgagaa gccccaggac ggcgcggcaa agcgtcgtct
     3901 tgcctgatcc gtttgggccg atgactccta cgaactcgcc ctcatagatg gtcaacgaaa
     3961 tgtcttggag cgcgatgagc ccgggaaatc cgaaggaggc atggtcgaaa cggatgatcg
     4021 gttcgcgggg atcagacacg gaatccgaag ctccttccgg cgccaagatg agaatagtta
     4081 cgaggtcgct tccaacgcac cggccaattg gagcacatca tagcgaagca tgtcgaggta
     4141 ggtctcggtt cccgccaaac cgcccggcat cgccgtcaag acgacgacgc gggccttggt
     4201 ctccctcgcc agcagctccg ggagtcgccg gctgagttga acctcggata cgacaacctt
     4261 gatgttttcc tttctaatct tctcaatgag aaattgcaga tgataggcgg atggttctgt
     4321 accggactgc ctatgaatcg tgccgacaat attgaagctg aatcgctttg ccaaataggg
     4381 ccaagccgga tgatgggcga tgaaacgccg gtcggacagc cgctgagtcc gctcgagcag
     4441 ttcctgttgc acctgaccca gccgtcggag gtaggccgtt tgattggcct ggtagtcagc
     4501 cgcatgacgt gggtccattt cgatgaaggc ttgggtgata tggcgcagca tgatggggac
     4561 attctcaggg tccagccaaa tatgcgggtt cccggcttcc ccttcatgag agtcagcgtc
     4621 gtctcgaatc agcgctactc ctcgggatgt cgtgatcaca cgtaacgatg ggtctcccgc
     4681 gttcttcacc agggatgaaa cccagacctc cagtcccatg ccgatctcca acaacagcct
     4741 ggccttccgg actgcgacca gatcggtggg tttgggtgaa taggtgtgct cgttttcgta
     4801 gccgctcaac aacgacgtga ctcgtacatg aggaccaccg acctgctccg ccaaatcctt
     4861 caggacggga atcgtgacca ccacaggaat cgggtcgcga gcctctgtaa tggtgccgaa
     4921 ggagaggagg aaacctgcca gcacggcaag gagagagaag acaggcgact tccgcacgtg
     4981 gttggccgga aacatcggcg gggacggtat catccgcgta ttccagtgtc aacgacatca
     5041 ggaaacgaca ggatgccagg agatcaccgt tcaaaagaaa cgccctcagg atgttcaaaa
     5101 aggccgtctt ttttcacccg cccatcccgg cgcgccaagg cgcgcctttc cacagccaag
     5161 gcctagcgag gtccgcgagg cgacgaataa ggagcctcac gtttgcggac gggcgcgagt
     5221 tggtgagcgc ccagtgtctt agaggcaaag cgtactttgg gccgtacgtt gaactctcta
     5281 aggttcacgc cgtgccgaat aaggcacggc acgcttgtga acgccgctga gatggtgagg
     5341 cggcagtttc ttcgcgagaa cgccgctggc ggactttttc aacatcctgt taggccctgt
     5401 ccattcgcct ccttgaccac acgccgaagt tcgattagcg tagcacacct gtgaacgtct
     5461 cgggatcgcg caggtggtga aaggataccg gcgatgaagg ttgtcggatt gatgtcagga
     5521 acgtcagccg atggagtcga tgcggcgctg gtcaccatcg cccgtcagag gtcagacctc
     5581 cgcgtcggga tgctcgcgtt tcattcgctt ccctacccgc gttcgctaca gcaacgaatt
     5641 ctttccgcat cggtttccgg aacggtgacg gatatctgcc acctcaatgc gctcctgggc
     5701 gaatggttcg ccgacgccgc cctaggagtc attcggtcgg ctcaattatc cccggaagac
     5761 atagacctga tcggatctca cggccaaacg gtgcaccacc tgccgcatgg catcaaagat
     5821 acgcgcgtcg gtgcaatacg ctccacattg caaattgccg agcccgcagt cattgccgaa
     5881 cggacgggga ttaccacggt ggccgatttc cgcccgcgcg acattgcggc gggcgggcaa
     5941 ggggcgccgc ttaccccagg gagccatgcc ctgctgtttc gacatccgcg ccgcgcccga
     6001 ctcatcgtga accttggggg catcagcaac gtcacctacc ttccccccgg atcaggctcc
     6061 gaggatcttg tcgcgttcga cacggggccg gcaaatatgg ttctcgatgg gctcatgtcc
     6121 cgcaccacga atggtcgatc ctcgatggac cgcgagggac gtacggcgac caaaggccgg
     6181 gtggattcaa gattgctggc caaacttctc gcacatccgt atctgtcgca ggcgcccccc
     6241 aaatcgaccg gtcgcgaggc gttcggcgaa aagatgctgg acgaattact caattggcag
     6301 caaacacgtc ggttatcggt cgaagatctt ctggccacct gtagtcgctg gacggccgaa
     6361 gcggtcggta cggccaggcg gtggatcagg ggaggaatcg atgacgtgat cgtgggtgga
     6421 ggcggtgtcc ggaaccgggc gatcatggga catttaacgg agatctttgc tcccgtgccc
     6481 gttacatcat ttgagtccca cggttgggat agtaaggctc tggaatcggt ggcttttgcc
     6541 gttctcgcgt atcagaccgt aatggaacag tgcgggaacg tgccatcggt tacgggggcg
     6601 gcgtctccaa gattgttggg gtgcatcgtc ccaagcggcc cacggtggta tgaacggctg
     6661 cgctcgcgca aaggcggcag taagacaaga tgaaaattct tttggccggt gcggtcatcg
     6721 ccgtgagcat cggtctttgg ggctatctga ggagggcgcg tcgaagaaag aaacaggcat
     6781 cgccgtttgc cgttcctcac gacgccacca tcagttccgt ccgattgctc gctcacgagg
     6841 aggtcgcgtt gtatagcctc ctacaaatgg ccgtccaaga gcgctacctc gtgttcagtc
     6901 aggtgccgct ctggtccttc atagaagtcg aagcagggga aaagatgcgt gccgatattt
     6961 tgcgccggat tgcgctcaaa cgagtcgatt ttgtcttggt ccaccccggg tcgtgcctcg
     7021 tcgaacaggt cgtgcagatc gagcgtgaat cttcgcaacc tcatgaagct gagcggcagc
     7081 acgtcaccga gtcggtgctc gacgccgcgg gaatcaagct gacgaagtta caaccgaagg
     7141 aaagccattc gctgcctgat ctcgcggcta gactgggatt agtcgaagag gaataacgac
     7201 gaaggctgct cggttagtag gacacgaggt cggctcaggt ccttgatgaa gatcgtaatg
     7261 ggatcgtcac gcacctcctt gtcgaagagg acattgacgt tggtcgtccg ggccagaatc
     7321 tcaaacacct ctttcagctt agcctcctga aagcggagtg tcaggggctc ttcggagcct
     7381 cagaacgtct tcgctgcacg ctgttgtttc gtgagctcgg taatgccgtc taacgcttta
     7441 ctctgggttg gatcgagctc cacggctcgt tcaaaggcag tgagggcttc ctctacgcgc
     7501 ccgagattct gcagcttctt ggcggtttgg agctgctcct ggctttcttt caaccgcagc
     7561 gcatctgcga tcatcgcatg gtattcggcc ttggaggggt cgagacccaa ggccagcttg
     7621 aactcatgaa gggcacccgc tagttgctcg ttctcaaatg cccgtttccc gagggcatag
     7681 tggtctgccg cggcagcagt cttcgcctgg tcgagctgcc gttgaatctt ctcatcgaat
     7741 ggggctttgc gctgtgcctc ttgataggcc gccacggcct ggtcccactg gccttccgct
     7801 gcgagacgat gggcttgtcg gagatcaggc gacacgcagc cggcgagagc acagaccacg
     7861 atggtgccga tgccgaatga tcggtggtac tgcatggttg gcctcacggc gatggcggtg
     7921 cgaaagcagc aagaaggaag gttgcaagaa ggctagatgt ttgcctggtc tctgagtacc
     7981 agatcgtgtg cgctagcctg aaaagtattg catacattgg agataccctg cccgttcaag
     8041 tgtatctcca aaccattggc cgtcaaattg gctcaggtat tgatttccca tagagcagcc
     8101 ttgttcagac agtctcatgg aggaaagtga caggaaggaa tccgttggat tcaatgcttc
     8161 ggcagaagat aatagaacgg gctgtggctt tcgcttgacc agcgcctttg cagtggccag
     8221 cgcgccacac tgagaatcgc cgcacggtgc ggtgcaaggg aagggagagt ggctggtgaa
     8281 cagggcctgt gccggagtct agagtggtca gagcaaatcc actcccctgc gctatgcacg
     8341 attcaagacc tgccctggac ttcccacttc actacctttc tcactgcaat tcacgctcta
     8401 tcgtacgacc tcaaatctgg ctccgtataa ctcagtgggg tccttaatat agaagaaaag
     8461 aaatctggtt ccccttctta tctccgttct tacaataagc gccccatcct gtgccaattc
     8521 aagccgatgg ataacgagcg cccgcccggc agtcgcctcc cctgtagtgt ccatgaggcg
     8581 ctcaattaca agctgttggt ctttgcacca cagcttgtcc cttggctgaa gttgcatggt
     8641 ctttataatt gaagacccaa tgatcctgac cgatatcgtg tgagtgtctg tctgcaccac
     8701 ttggatatca ctcgtcggcc atcgttctgg cacgttgagg tccaggctca gcataacatc
     8761 cagggctaat ccactactga acctggaatg cggaggcatg ccgggaagcg cttccccata
     8821 caatttatag gtcccactga cattcgggca gtcgtcatga ctcgcgtgct ccatataagc
     8881 atcttccgga ataacgttcg taccacctaa cccacatgac gtacaaagca ggcataacag
     8941 gatcgttctc cagtaagagg gttcaacacg tatgagcttc aaccagttgc tcgtctcaaa
     9001 agttctcatt gttgaacatt atcgggacat gtacggatat gcctctcctt gaatcagt
//
LOCUS       DATNDD010000114         5819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4095697, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000114 DATNDD010000000
VERSION     DATNDD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095697"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VN647_02760"
     CDS             complement(<1..839)
                     /locus_tag="VN647_02760"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWG95982.1"
                     /translation="MSEKLELYVYTTHSYAKKSYLKVGHCKRGRHLERIKEQFGTSNP
                     EQPLIRFVEDLPEGKTDLHIHQQLRANGIDKVEDSVGQEWFVATLDDVKRAFNEVCFG
                     SSRIENYRLRKEQQVAIDKAVRWFHGGYSPEVMDSAAHKDRFLLNAKMRFGKCFTGIH
                     IAKALGARKTLIVTYKPEVMSEWVGAINDHIDFEGWTGIRARKNEDNINDPHLPDSGE
                     LPKTTKPFVLCVALQDLHINADGLTKRRLRHVLTVHWDLIIFDEVHFGSRTERAKYII
                     ENP"
     gene            complement(839..2302)
                     /locus_tag="VN647_02765"
     CDS             complement(839..2302)
                     /locus_tag="VN647_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HWG95983.1"
                     /translation="MSACKILTPDSHVVDILGSTKGRDQPIMLVGKALELATCISESA
                     LLDQNTVILDPFCKAGEILLAAALRSCVVRKGRSKPLVSSREVSEEIYGGRYYALSPD
                     ERHYLLSLRTFYGNERSHDKGFARHIRNGNYLSEIDGRLNLTKFRGELKTMIQFIRKT
                     KPNCKVIAVGNPPYQEADEGFGGSATAIYNHFVEALMDTEEISEFAVVIPSRWFSAGK
                     GTEEFRQRILASKQIKAIRHFKQSKEVFPTVDVLGGVCFFHFKRAHNGNTLFIDSEAS
                     CDIDLSKFDIITDDPSAYPLIEKIQSKWRGQFVSDIAWPGKPFGIRTFYFKRNRQIDP
                     THKNAVPCFSKGRKIRYVKRSEISKNTDKIDLFKVAVPAAYAPGSRMGVRRVTLPRNQ
                     YFIIPKGTITAETYNIVGAFKTKKEAENFLIYLQTDFARYLLGLRKLTQHIPQDRWNW
                     LPYMDPAVEWTDKSLFDFFKLTKEEREHIKKKVQEWS"
     gene            complement(2302..3003)
                     /locus_tag="VN647_02770"
     CDS             complement(2302..3003)
                     /locus_tag="VN647_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95984.1"
                     /translation="MGEVFTPEQYVQQMLVLFDEKLWSDENTVFFEPACGHGNIALAI
                     VERRISALVKKYVKAGIDQPVLHAVATTIHTIWAVDICPLNVHLTRKRIIDMVVRRLR
                     TTDFEIRQPELNGYLIHVLCTLIWQIHENETLSALSCQDTAQARASQTKIGDSWVKAN
                     SHKPINFDLNWCEFYERSTARNTVPLLYEKTARFLETSIGGGNTRGFEDFNFARDAVQ
                     LLIENHHTQPTQEAA"
     gene            complement(3455..4288)
                     /locus_tag="VN647_02775"
     CDS             complement(3455..4288)
                     /locus_tag="VN647_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWG95985.1"
                     /translation="MSRRRTVSWPDRMRSCIGSCLSEPFYRTFSLVPYWEWGLSVHDV
                     SVLTQQHPPLAGCRAVHLTDLHLDRYYPRHNRIVESVKELHPDWIFITGDLLNVPEGL
                     PHLFRFLDQLRAIAPLYMTLGNHDHYSGVPMSQFSELADQHKITLLVNQSTVVSTGRG
                     ELAIVGVDDPSLHRADLGCVPSRVDSRFTVLLAHAPNILDYVEPHHAVDLILCGHSHG
                     GQWRVPAIPIFWLPPGCNGRVAGWHESGPHRLYVNRGLGWSFLPFRLNCRPEIAVIEW
                     V"
     gene            4413..5459
                     /locus_tag="VN647_02780"
     CDS             4413..5459
                     /locus_tag="VN647_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HWG95986.1"
                     /translation="MTLPIDPFLTGNLPGIGGKIRTVPEDFQVDERPLYLPCGEGEHL
                     YVTITKRGLSTPDLVRRLSSTLCVKAQAIGVAGLKDARAVTTQMVSLQGIRPEQISRL
                     KIDDTILNVQILGRHRNRLRTGHHSGNRFCLVIRHVADHAAETVPAVLQQLSTRGVPN
                     YFGPQRQGKNSENYRIGALLLHDARRREKMSRATRIWYLNSYQSFLFNRILARRIDHL
                     DRVFVGDWAMKSDNGACFQVVDAEKEQPRADRFEISPTGILFGSRVSWASGEPGQIEE
                     TVIAEAGATKEGLVAAAKACGFRGERRALRIPLAELEWSLRDDALTLSFSLPAGAYAT
                     SVLRELMKVSPTKP"
     gene            5526..>5819
                     /locus_tag="VN647_02785"
     CDS             5526..>5819
                     /locus_tag="VN647_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HWG95987.1"
                     /translation="MNHYQESVYLQGHIIDSLVLAKVLDLILMMGGTFDLEDVHIGKT
                     RAEPSHARIRIRTASRALLDDILRKIQPHGASIEREADCRTDRAPADGVLPDDF"
BASE COUNT         1355 a         1513 c         1494 g         1457 t
ORIGIN      
        1 tctggatttt cgataatgta tttcgcccgc tcagttcggc ttccgaaatg cacttcgtcg
       61 aagatgatga gatcccagtg tacggtcagc acatgcctaa gccgacgctt ggtcaaccca
      121 tctgcattga tgtgcaggtc ctgcagggct acacagagga caaaaggctt ggtggttttg
      181 ggaagctcgc ctgaatctgg caagtgagga tcattgatat tgtcctcatt cttccgcgcc
      241 cggattccag tccatccttc aaagtcaata tgatcattga tggcgccgac ccactcggac
      301 atgacctccg gcttgtaggt gacaatcaag gtctttcttg caccaagcgc cttggcaata
      361 tggatccctg tgaagcattt tccaaagcgc atcttggcat tcagaaggaa gcgatccttg
      421 tgggcagcac tgtccattac ttctggcgaa taaccgccgt gaaaccatcg aaccgcctta
      481 tcaattgcta cctgctgttc tttcctcaac ctgtagtttt cgatacgcga tgaaccaaaa
      541 cacacttcat tgaacgcgcg tttcacgtca tcaagtgtgg ccacaaacca ctcttgacca
      601 acgctatcct ctactttatc aattccgttg gcacgaagct gctggtgaat gtggaggtcg
      661 gtcttccctt caggaaggtc ttccacaaaa cgaatgagtg gctgctcagg attgcttgtg
      721 ccgaactgtt ccttgatgcg ttcgagatga cggccacgct tgcaatgccc aacctttagg
      781 tagctcttct tcgcatagga atgggtcgta tacacataca gctcaagctt ctcgctcatt
      841 acgaccactc ctggactttc tttttgatgt gttcccgctc ttctttggtg agcttgaaga
      901 agtcgaacag tgacttgtcg gtccattcaa cagcaggatc catgtatggc agccagttcc
      961 aacggtcctg tggtatatgc tgagtgagtt tccgtagacc cagcagatat cgggcaaaat
     1021 cggtttgaag atagataaga aagttttctg cctctttctt tgttttaaat gctccgacta
     1081 tgttgtacgt ttccgctgtt atcgtgcctt tggggataat gaagtattga tttcgaggaa
     1141 gcgttactcg tctcacgccc attcttgatc caggggcgta ggctgcgggg acagccactt
     1201 tgaacaaatc gatcttgtcg gtgtttttgg agatctcgga ccgtttcaca tatcggattt
     1261 ttcgcccttt tgaaaaacag ggcacagcat tcttgtgcgt aggatcgatc tgccgatttc
     1321 tcttgaaata gaaagtcctt atgccaaatg gctttcctgg ccaagcaatg tcagacacaa
     1381 attgaccgcg ccatttagat tggatctttt caatcaatgg gtatgcactt ggatcatcgg
     1441 taatgatgtc gaactttgaa agatcgatat cacagcttgc ttcactatca atgaaaagcg
     1501 tattgccatt gtgcgctctc ttgaagtgaa agaagcacac tcctccaagt acatccaccg
     1561 tcgggaatac ttctttcgac tgcttgaagt ggcgaatcgc cttaatttgc ttagaggcta
     1621 gaatcctctg ccggaattct tctgtgcctt tgcctgcact aaaccatctt gagggtatga
     1681 caaccgcaaa ttcggagatc tcttctgtgt ccatcagcgc ttcgacaaaa tgattgtaga
     1741 tcgctgtggc acttccgccg aagccttcgt ccgcttcttg atagggtgga tttccgaccg
     1801 caataacttt acagtttggt ttggtctttc taataaactg aatcatggtt ttcaattcac
     1861 ctctaaactt ggttaaattg agtcgcccat caatctcaga tagataattc ccattccgaa
     1921 tgtgcctcgc gaatcctttg tcgtgcgatc tttcattgcc gtaaaaagtt ctcaggctta
     1981 acaggtagtg cctttcatcc ggggagagag cgtaatagcg accgccgtat atttcttctg
     2041 agacctcacg tgaggataca agcggtttgg atcgcccttt gcggactacg caggatctga
     2101 gtgctgcagc cagcagtatt tcgcctgcct tgcaaaaggg gtccaagatg acggtattct
     2161 gatcaaggag cgctgattct gatatacagg tagcaagttc cagtgctttg ccgaccagca
     2221 tgattggctg gtctcggcct ttcgtactac caagaatatc tacaacgtgt gagtcgggtg
     2281 tgagtatctt gcaggcagac atcaggcggc ttcttgggtt ggctgagtgt ggtgattctc
     2341 aatgagaagt tgcactgcat cgcgagcgaa gttgaaatct tcgaagcctc gtgtattgcc
     2401 accacctatt gaagtctcca gaaaacgcgc tgtcttttcg taaagcagtg gcaccgtatt
     2461 gcgggctgtg gagcgctcat agaattcaca ccagttcaaa tcgaaattga tcggtttatg
     2521 gctattcgcc ttgacccatg agtcgccaat ctttgtctgc gatgccctgg cctgagctgt
     2581 gtcttgacag ctcaatgcag aaagggtctc gttctcgtga atttgccaaa tgagagtgca
     2641 gagaacgtgg atgaggtagc cgttcagttc tggttggcgg atctcgaagt cagttgtccg
     2701 aagcctccgt acaaccatat cgataatgcg tttgcgagtc agatggacgt ttagagggca
     2761 tatgtccacc gcccatattg tgtgaatcgt agtagccact gcgtgtaaga cgggctgatc
     2821 aattcccgct ttcacgtatt ttttgacgag agcacttatt cggcgctcca caattgccaa
     2881 ggcaatgttg ccgtgaccac aggcaggctc aaagaagacc gtattctcgt ctgaccagag
     2941 tttctcatcg aacaagacaa gcatttgttg gacatactgc tcgggggtga agacctcacc
     3001 catgtctttg actcgttctc gattgcggtg atgttcaatg gtggttagaa cggaatgctg
     3061 atcgcttgct ttatctggat ctgatgaaag ctttcggtgt cgcttcacaa agggacgaca
     3121 ggttcgtggc tggattggta ccgctcttcc ggttgtaaac atccttaatg acctgctctt
     3181 ctgctcatga ctcaagcagt aatgcttacc tgtactttct tcggcgtatc gggatgcgga
     3241 ctttagtaat gcggggaaag gacgtaccgg aagaactgag gcagtgccat ccagtcgtga
     3301 agaaactaag gaatctggaa tctttttggg gaagtgaagg agtgatgcac gtgagaggcg
     3361 atgtcggcgt ggttagacta gtgggctccc gccctgggcg atataaacag gctgccggta
     3421 gtcatgagat ctgattccac ccttcgggct gtccctaaac ccactcaatc accgcaattt
     3481 caggccggca gttcaaacga aatgggagga aggaccaacc caaccctcgg ttgacgtaca
     3541 gtctgtgcgg gccggattcg tgccaccccg ccacgcggcc attgcagcca ggaggaagcc
     3601 agaagatggg gattgccggt accctccact gcccgccatg actgtgtcca cagagaatca
     3661 agtcaacggc atgatgcggc tcgacataat ccagaatgtt gggagcgtgt gccaacagca
     3721 ccgtaaaacg agagtctact cgagacggga cacatccaag gtccgcgcga tgaagcgatg
     3781 gatcatcgac gccgacgatc gccagctctc cccggcccgt tgaaacgacg gtactttggt
     3841 tcaccagcaa cgtgatcttg tgctgatcgg caagttcaga aaactgtgac ataggcactc
     3901 cgctataatg gtcgtgattg cccaaggtca tgtacagagg cgcgatggcg cgtaactggt
     3961 cgaggaatcg aaagaggtgg ggcagacctt ccggtacgtt gagcagatcc ccggtgatga
     4021 aaatccaatc agggtgaagt tccttcacgg attcgacgat acgattatgc cttggataat
     4081 atcggtccag gtgcagatcc gtgagatgga ctgcgcggca accggcaagg ggcggatgtt
     4141 gttgagtgag gacggaaacg tcatgaacgg acaaacccca ttcccaataa ggaaccaggc
     4201 tgaacgtccg atagaacggc tcgcttaaac aagagccgat acacgatcgc atgcgatcag
     4261 gccatgacac cgtccgtcgc ctgctcattc cgcgacctgc cgtttgcctg atcgtttccg
     4321 catggacgag aaaagtatac catgggcttg tttctcatca cgtttgacga cacatattct
     4381 cggacagccg tcattccaac ggaggaaaac gtgtgacgct accaatcgac cccttcctca
     4441 ccggcaactt gcctggaatc ggtggaaaga tccgcaccgt gcccgaagac tttcaagttg
     4501 acgaacggcc gctctatctt ccttgcgggg aaggtgaaca tttgtacgtg accatcacta
     4561 aacgtggtct ttccacgccg gatctggtcc gtcgactctc ttctaccctg tgtgtgaaag
     4621 cacaggccat cggcgtggcg ggcctgaagg acgcacgagc cgtcacgact cagatggtgt
     4681 ccttgcaagg aatccgcccg gaacaaatct ctcgcctcaa gatcgatgac actatcctca
     4741 acgtgcaaat cctcgggcgc catcgtaatc ggctacgaac cggccatcat tccggcaatc
     4801 gcttctgcct agtcatccgc catgtcgccg atcacgcggc tgaaaccgta ccggccgtcc
     4861 tccaacaact gagcacacgc ggcgtgccca actacttcgg ccctcagcgg caagggaaaa
     4921 acagtgagaa ctatcgcatc ggcgctctat tgttgcacga tgcacggcgg cgtgagaaga
     4981 tgagtcgggc cacccgcatc tggtatctca attcttatca atcattctta ttcaatcgga
     5041 tacttgcgcg acggatcgac catctcgatc gagtcttcgt cggtgattgg gccatgaagt
     5101 cggacaatgg cgcctgcttt caagtcgtgg atgccgagaa ggaacagccg cgagcggatc
     5161 gtttcgaaat cagcccaaca ggcatcctct ttggttcgcg ggtatcctgg gcaagcggcg
     5221 aacccggtca aatcgaggaa accgtcattg ccgaagcagg cgcaacgaag gaaggcctcg
     5281 tcgcagccgc gaaggcttgt ggattccgtg gcgagcgtag agcgcttcgc atccctctcg
     5341 ctgaacttga atggtctctt agggacgatg ccctcaccct ctcgttcagt ttacccgccg
     5401 gcgcctatgc tacgagcgta ctcagggaac tgatgaaagt atctcccacg aagccttagt
     5461 tcctctcatt ttcttcctcc agccaccgcc ggtggtaaaa tatgtcatat acagtgagta
     5521 ccgcgatgaa ccactaccag gaaagcgtgt accttcaagg gcacatcatc gactctctcg
     5581 tgctcgcgaa agtgctcgat ctcatcctaa tgatgggggg aaccttcgac ctggaggatg
     5641 ttcacatcgg caagaccagg gcggaaccat ctcatgcccg tattcgaatc cgaaccgcct
     5701 cgagagcgct cttagacgac attctgagga agattcaacc tcacggcgcc tcgatcgagc
     5761 gtgaggcgga ttgccggacc gatagggcac ccgccgacgg agtgttgccc gatgatttc
//
LOCUS       DATNDD010000115         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1550707, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000115 DATNDD010000000
VERSION     DATNDD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550707"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN647_02790"
     CDS             <1..322
                     /locus_tag="VN647_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95988.1"
                     /translation="MMLAKSGKSFDATLLKLFVNCVGIIPIGSLVVLDSDEFAVVLKP
                     AADKAESERPVVKVIADAQGTPLDNGPELDLTEKDGVGEYRHSIIRLIDNTEHKFDTS
                     RYFL"
     gene            407..1351
                     /gene="folB"
                     /locus_tag="VN647_02795"
     CDS             407..1351
                     /gene="folB"
                     /locus_tag="VN647_02795"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWG95989.1"
                     /translation="MGAHIVIQQLEFRGRCGVMPEERARQQPLAVDLDLELDGRLEAA
                     GLSDDLVHTIDYAKVAQHVIDIGTLQESQLLETLAERLLSVLFDKFPIGRITLWLRKL
                     HPPISFITRSVGIRIERTRLAQQVISADPPPAEFLIRQLHRLPKGKVLDVASGSGRHA
                     LFLASLGYQVEAVDRDEQAFVQLSAVARARHLTGINLQVLDLEQPSHEPNLGHEAYDV
                     ILVFFYLNRPLFPLLIDALKPGGVLLYETFTVDNHVQHRHPKRREFCLASCELLSLTL
                     GLRILHYDEGLHEGTSRSESVYTAQLMAQKPLSPGPSI"
     gene            1348..2196
                     /locus_tag="VN647_02800"
     CDS             1348..2196
                     /locus_tag="VN647_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWG95990.1"
                     /translation="MSRLDLHLHTTHSDGSCTPTEVVNMAHRAGVTALAITDHDILTG
                     VAEAIAVGQQCGIEVIPGVEISSLAGNSELHILGYFLDCRNPDLLERLKTLRDGRHLR
                     NPRIIERLHTLGIDITYDEVRALAGTGSIGRPHIARVLMDKHVVASAKEAFDRFLAEG
                     KPAYVPRELPHPAEAIRWIKAARGLAVLAHPTWVKVTEQPLVDLVRQLKADGLDGVEV
                     YYSTHAARQTREYLSLAQQLGLLVTGGSDFHGLTKPDIEVGIGKGALHIPASLLPKMK
                     EAAGRL"
     gene            2283..3680
                     /locus_tag="VN647_02805"
     CDS             2283..3680
                     /locus_tag="VN647_02805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWG95991.1"
                     /translation="MVNASSWAVQYVLVALVALFAGPVLSKLPAAQSVPFSLFGLTGS
                     QTIRLIVVGIGLVALCILSLRAFRQMPDNGRGFSFLRQLVPPVTALFIVIVTDKTIQV
                     VGLSLLDPVGTIRYTLAYAASLTLIGFWLTTAWLLNLDALHRFFERPVQSTRRRGPAQ
                     LIDDAEEAHHDPECSEPETTAHISTAQNGPPATLGRYKVLKELGRGSMGVVYLGKDPT
                     IQRFVAIKTMQLDEIDDADKLQEVKARFFREAESTGRLSHPNIVTIFDAGEEDDLGYI
                     AMEMLEGTTLKQWSRKPNLLPLEKVIPILATVAEAMDYAHQQGVVHRDIKPANIMLTT
                     DEVVKIMDFGIAKMATSSKTQTNIVLGTPTYMSPEQIAGKKVDGRSDIFSLAVVMFEL
                     LTGRPPFTADNVSALLFAIAHTPHLSVKAIRPDLPPIIKEVLDRALQKDPVHRYRRAG
                     EFAMELRSCLAGLAA"
     gene            complement(3848..>4099)
                     /locus_tag="VN647_02810"
     CDS             complement(3848..>4099)
                     /locus_tag="VN647_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95992.1"
                     /translation="RRRCVLQRLKRLDVPCRQIVRQRLVRWKEAEGYKLACIEDVKRV
                     CLTVQPGDGRILQCLQEHEQDLSEACYQSLPKGRLNLRN"
BASE COUNT          889 a         1256 c         1052 g          902 t
ORIGIN      
        1 tatgatgctt gcgaaatccg ggaaaagttt cgacgcgacc ctgctcaaac tcttcgtcaa
       61 ttgcgtggga atcattccga tcggaagcct ggtggtcctc gactccgatg aattcgccgt
      121 cgtgctcaag cctgccgccg acaaggccga gagcgaacga ccggtcgtca aagtcatcgc
      181 cgatgcgcag gggactcccc tcgataacgg tcccgagttg gacctgaccg agaaagacgg
      241 cgtcggcgag taccgtcaca gcatcatacg cctgatcgac aataccgagc acaaattcga
      301 tacgagccgt tacttccttt agccgttgta aacggcacct gtcggctccc aaacccatct
      361 ctcttcctcg cttgacagcc tgaaaccgat ctttgacaat gtgtacatgg gtgcacatat
      421 cgtcatccag caattggaat tccgcggccg ctgtggcgtg atgcccgagg aacgcgctcg
      481 ccagcaaccg cttgccgtcg atctggattt ggaattggac ggtcgtcttg aggcagcagg
      541 attgtccgac gatcttgttc acacgatcga ttacgcgaaa gtggctcagc atgtcatcga
      601 cattggaacc cttcaggaat ctcagctgct ggaaacgctg gcggaacgac tcctgtcagt
      661 cctgttcgat aaattcccca tcggtcgtat cacgctgtgg ctgcgaaagc tgcatcctcc
      721 gatttccttc ataacccgct ccgtcgggat ccgaattgag cgcacaaggc tcgcgcagca
      781 agtcatcagc gcagatccgc caccggccga gtttctcatc cgacagcttc atcgccttcc
      841 caaagggaag gttctcgatg tggcctccgg cagcgggcgt cacgcactgt tcctcgcctc
      901 gctgggctat caggtcgaag ccgtcgatcg cgatgagcag gccttcgtac aactttctgc
      961 cgtcgcccga gcacgacacc tcaccggcat caacttgcaa gtgcttgatc ttgagcagcc
     1021 gtcacatgaa ccgaatcttg gacatgaagc gtacgatgtc attctcgtct tcttttacct
     1081 gaatcgaccg ctcttccccc ttctcatcga cgcattgaaa ccaggcggtg tcttgctgta
     1141 tgaaaccttt accgtcgaca accacgtcca acaccgacat ccaaaacgtc gagaattttg
     1201 cctcgcatcg tgtgaactct tgagcctcac tctcggcctc cgcattcttc attatgacga
     1261 agggctccac gaaggaacga gcagatcgga gtccgtctac acggctcaac tcatggcaca
     1321 gaaacccttg tctccaggac cgtcgatatg agccgtctgg atcttcatct gcataccacc
     1381 cactccgacg gaagctgcac acccactgag gtggtaaaca tggcacaccg cgcaggggtg
     1441 acggccttgg cgatcaccga tcacgacatc ctgaccggtg tcgccgaagc cattgcagtc
     1501 ggacaacagt gtggaatcga ggtcataccc ggagtggaaa tcagttcgct tgccggtaac
     1561 tcggagttac acattcttgg gtactttctt gattgccgga accccgattt gctcgagcgt
     1621 ctgaagaccc tgcgagacgg tcgtcatctc cggaatccac ggatcatcga acgattacac
     1681 accttgggaa tcgacattac gtacgacgaa gtccgcgcac tcgccggcac cggctcgatc
     1741 ggcagaccgc acatcgcccg cgtcttaatg gacaaacacg tcgtcgcttc tgccaaagaa
     1801 gcctttgatc gttttcttgc cgagggtaaa ccggcctacg ttccccggga gctcccgcac
     1861 cctgccgaag cgatccgctg gatcaaagcc gctcgcggac ttgccgtcct ggcccacccg
     1921 acgtgggtga aggtgacgga gcagccgctg gttgatctag ttcgacagct caaagccgac
     1981 gggctcgacg gtgtagaggt ctactatagt acgcatgctg cgcgacaaac tcgggaatac
     2041 ctcagtctgg cccaacaact cggattattg gtcaccggtg gcagcgactt tcatggcctc
     2101 acgaaacccg acatcgaagt cggcatcggc aagggcgcgc tgcacattcc agcctccctg
     2161 cttcccaaga tgaaggaagc cgccggacga ctctagcgca gtatcctccg cgtattttcc
     2221 ccaatcgatt gactcctctt cgatatccgc tagactctga ttatcttcaa atctcatcat
     2281 ccatggtcaa cgcatcgagc tgggccgttc aatatgtact tgtcgcactg gttgcgctct
     2341 ttgccggtcc cgtactgagc aaactgccgg cggcgcagtc ggttcccttc tcgctgttcg
     2401 gcctgaccgg ctcccaaacc attcgcctca tcgttgtagg aatagggctg gtcgcgctct
     2461 gcattctttc gctccgcgcg ttccgacaaa tgccggacaa cgggcggggt ttctcgtttc
     2521 tacgacaact ggtccctccg gtcacggccc tgttcattgt gatcgtaacg gataagacca
     2581 tccaggttgt cgggctttcg cttctcgacc ctgtgggaac catacgctat acgctggctt
     2641 atgctgcgtc attgacactg atcgggttct ggcttaccac ggcatggctc ctgaatctcg
     2701 acgcactcca ccgttttttt gagagacccg tacaatcaac tcgtcgcaga ggtcctgctc
     2761 agctgatcga cgatgctgag gaagcccacc atgatccgga atgcagcgag cctgaaacca
     2821 cggcccacat ctccaccgca cagaacggtc cacccgctac actgggtcga tataaagtat
     2881 tgaaggaatt gggacgagga tcaatgggcg tggtgtatct tggaaaagat ccgacgatcc
     2941 aacggttcgt ggcgatcaag acgatgcagc tcgacgaaat cgacgatgcc gataaactcc
     3001 aggaagtcaa agcgcggttt tttcgggagg ccgaatccac cggacgtttg tctcatccaa
     3061 acatcgtcac cattttcgac gccggcgagg aggatgacct cggttacatc gccatggaaa
     3121 tgcttgaagg caccacactc aaacagtggt cacgaaagcc gaatctcctg ccgctggaaa
     3181 aggtgatccc gatactggcg accgtcgccg aagcgatgga ctatgcccac cagcaaggcg
     3241 ttgtacatcg cgacatcaaa ccggcgaaca tcatgttgac cactgacgag gtcgtcaaga
     3301 tcatggactt cggaatcgcc aagatggcga cgtcgtcgaa aactcagacg aatatcgtgt
     3361 tgggaactcc cacgtacatg tcccccgaac agatcgccgg gaagaaggtg gatgggcgat
     3421 cggatatttt ttcgctcgcc gtcgtgatgt ttgaacttct gaccggacga ccgccgttca
     3481 ctgcagacaa cgtctcggcg ctgctgtttg ccatcgctca cacaccgcat ctcagcgtga
     3541 aagcgatcag acccgatctg ccgcctatca taaaggaagt tctggatcga gcgttgcaga
     3601 aagacccggt ccaccgctat cgccgcgccg gcgagttcgc catggagctt cgctcctgcc
     3661 tcgcagggct ggccgcgtaa atgctacctg cgctccttct ctccattctc cacggaagtt
     3721 tagtttctac acgggagttt aggtttccag ccaatctttc cgatcgctgt ctatgaatcg
     3781 cgccacgccg gcagtcgaaa gcgacaccgg caaagctgtg cggcttgccg tacctgatgt
     3841 acgatcttca gtttcggaga ttgagtcgcc ctttgggcaa gctttggtag caagcttctg
     3901 agagatcctg ttcatgttcc tgcaaacatt gcaggatgcg gccatcaccc ggctgcaccg
     3961 tcagacagac ccgcttcaca tcttcaatgc aggcaagctt atatccttcc gcttccttcc
     4021 agcgtaccag ccgttgccgg acgatctgtc gacacggcac atccaatcgc ttgagacgct
     4081 gcagcacgca acgccgcct
//
LOCUS       DATNDD010000116         6301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4223727, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000116 DATNDD010000000
VERSION     DATNDD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4223727"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VN647_02815"
     CDS             <1..281
                     /locus_tag="VN647_02815"
                     /inference="COORDINATES: protein motif:HMM:NF040250.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95993.1"
                     /translation="SNKLEIDKAGKISILLLRVGEPQQGVVGLQKAGVAGELEPGLSV
                     RYMGTNDHGIASHLITRYFSTAVLTEDAIARLDNVSTKFYHEYVHPKG"
     gene            286..1437
                     /locus_tag="VN647_02820"
     CDS             286..1437
                     /locus_tag="VN647_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWG95994.1"
                     /translation="MTSAHNPISHDDDSFATTLIARLANELYRDSAITTQSSSPISEA
                     SFVSPVAPAALAGIPGGLQGMAGTHSTFPTSFATFPTDKPDSPGLAGGYPLATTTTHA
                     PIHADHVEPPAPPRSGNELGSGEHQPNSNEVYPFGEPRCNGSFRAREVYADAVDTSSA
                     FYFLRENGSHTGSKDQELSVGPTLSLREVDVIRRDFPVLHQRVHGHPLIWFDNAATTQ
                     KPRSVIDATSRFYGHDNSNIHRAAHTLAARATELYEGGREKVRQFIGAADTKEIVFVR
                     GTTEAVNLVAQTYGRSYIGRGDEILLTVMEHHANIVPWQILAEQTGALLRVAPINDAG
                     ELILEQFAPLLNPPHQISSGHASLERLGDDQPSGGDHWNGSCSRGTGSG"
     gene            1367..1648
                     /locus_tag="VN647_02825"
     CDS             1367..1648
                     /locus_tag="VN647_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWG95995.1"
                     /translation="MGTINPVEAIIGMAHAQGVPVLVDGAQSAPHLPINVRAMDCDFF
                     VFSGHKIFAPTGIGILYGKAALLEKMKGKVLPCSTEIFCLQLFVMSGRI"
     gene            complement(1939..2313)
                     /locus_tag="VN647_02830"
     CDS             complement(1939..2313)
                     /locus_tag="VN647_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG95996.1"
                     /translation="MHTFLHRRVVSTVLLIASLSVSLPVSANPPTKERLPLMLSGSGC
                     DSKEAEMNNVLQAIPGVTGVYFNRIPDHVLVDITPSAVKPADVVKRVNDAAISWQCKV
                     ELMQSCITADMSSTSAAMPHSE"
     gene            complement(2386..2838)
                     /locus_tag="VN647_02835"
     CDS             complement(2386..2838)
                     /locus_tag="VN647_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWG95997.1"
                     /translation="MSPKFLSPFLMVSLFGFTCFPFHSVAEEGVIVDGAGYTLHDTES
                     IKGHDEQHVSKDPVCDSSRKPKILSVEPDEAKPGHTITIKGENFGTRDCFRGVAFSAA
                     GPTQIDYKFVSDTTVEATVPDVRPGMSFIDVVAGGGTARSKAFLVQAK"
     gene            complement(2861..3682)
                     /locus_tag="VN647_02840"
     CDS             complement(2861..3682)
                     /locus_tag="VN647_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWG95998.1"
                     /translation="MTVGQFVTGLLVGVSLCVSHAGTFAQTAGNLIIVGNGPEQTIIE
                     ALARAFEKANPRAYVDIQWDEDCKPLQSVKTGQAHIAVTGVEDPALAATQIGWDGIGI
                     LVHLSNFTKEVTKQQVADIFSGKIKEWSDVGGPETKILLIDRPRNQNVREAFETQLGI
                     ADKIPDSTKMIGVDEAVVKTVVGTLPPLSAVAYISLSTGLSVVSGGVAVRLLPVDKVE
                     PEVPTVKDGRYSLRRPLLLLSKNESNTLVEAFAKFALSPPGQTIIGEIYIPMPNK"
     gene            complement(3732..4046)
                     /locus_tag="VN647_02845"
     CDS             complement(3732..4046)
                     /locus_tag="VN647_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWG95999.1"
                     /translation="MPLYEYRCGQCEKQFEANQSVYARVEDTECPFCRARQATRLMSS
                     FSSNVVGDRKPGFSELKAKAMNEERLQRFAKLPPLNIRPNMLSPHVTSESEPPSTEGS
                     TS"
     gene            4198..4374
                     /locus_tag="VN647_02850"
     CDS             4198..4374
                     /locus_tag="VN647_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96000.1"
                     /translation="MPVLIRRYKGNNGMMQEERIDDDDRIERYMRLFEKDDVKKLETG
                     VKVFIEKDEWQLLP"
     gene            4449..4652
                     /locus_tag="VN647_02855"
     CDS             4449..4652
                     /locus_tag="VN647_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96001.1"
                     /translation="MIYWVHIARAIDRVTLHRDRCSEVPSTATSSDFWEGGWFDYPDK
                     ERALRAMEQAGTNDQRRCPLCKP"
     gene            4752..5576
                     /locus_tag="VN647_02860"
     CDS             4752..5576
                     /locus_tag="VN647_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWG96002.1"
                     /translation="MPRLALLLTTVIWGATFPATKAALEQIPPLSFLLLRFLLGTLLV
                     LLWFVVSRRRLHRDRVVLGASALTTVFLFLGYLLQTVGLRHTSASNSAFLTALYVIFV
                     PMILMRIDSRVVSATAIAVVGLWLLVKPDTSMNLGDLMTLGCAVAFAGHIVCLERFTR
                     QVDAPSLFVWQMMAMTLLFLPAPWWEETTQSDFSPTAVLLIGLGVTGILATLAFAVQI
                     WAQRLVPAQQVALLFASEPAYAAWLSWYFLGETLDVQGWIGSALILLAVAIGAFGA"
     gene            5600..6070
                     /locus_tag="VN647_02865"
     CDS             5600..6070
                     /locus_tag="VN647_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96003.1"
                     /translation="MAQKFGNGRWVCKGFLDNRVDGTVVGQVVFAVIGPVDFYLRGNF
                     KSDIAGEVIRFHNSRFEDDDMAGQVIGDMANQQVGTVNLISLDPHPNLEPHPYVEWFT
                     LRKDHYRFELSPGDAWVLSDEERKTIDGESRRIRETLADRVTDQPRSDDGSEWV"
     gene            complement(6067..>6301)
                     /locus_tag="VN647_02870"
     CDS             complement(6067..>6301)
                     /locus_tag="VN647_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96004.1"
                     /translation="AATRQGFYWWEIDMTYYCLKGLEWMGLVWDIRGVPGYVREGKTK
                     LDSDRSVLKKKIDAAVKAAELPAPQPQLELTPP"
BASE COUNT         1383 a         1646 c         1788 g         1484 t
ORIGIN      
        1 cgagtaacaa gttggagatc gataaggcgg gcaagatcag catcctgttg ttacgtgtgg
       61 gtgaaccaca gcagggtgta gtcggtctac aaaaagccgg tgttgccggc gaactcgagc
      121 ctggtctgtc tgtccggtat atgggtacca acgatcacgg catcgcgtca catcttatta
      181 cacggtattt ttccactgcc gtgctgacgg aggacgcgat cgcccgtctc gacaatgtct
      241 cgaccaagtt ctatcacgag tatgtccatc cgaaaggctg acaccatgac cagcgctcat
      301 aacccaatca gccatgatga cgattcattc gccacgacgc taatcgcacg attagcgaat
      361 gagttgtaca gggactccgc catcacgacc cagtcatcgt ctccgatttc tgaagcatca
      421 tttgtgtcac cagttgcacc ggctgcactc gcgggaatac cgggaggctt acagggcatg
      481 gccggtacgc actccacatt cccgacatca tttgctactt tcccgactga caagccggac
      541 agtcccggtt tagcaggcgg gtatccattg gccacgacga cgacacatgc gccgatacat
      601 gcggatcatg tggaacctcc cgctccacct cggtcaggaa atgaacttgg ctccggagaa
      661 catcaaccga actccaatga ggtgtatcca ttcggggaac ctcgttgcaa cggcagtttc
      721 agggcacgag aagtctatgc cgatgcggtg gacacctctt cagcattcta ctttctacgt
      781 gaaaacggct cacacaccgg ttccaaagac caagagcttt cggtcggtcc cacgttgtcg
      841 ctacgcgagg tagatgtaat ccgacgggat tttcccgtcc ttcaccagcg tgtgcacggc
      901 cacccgctta tctggtttga caacgccgcc accacgcaga agccgcggag cgtcatcgac
      961 gccacgtcgc gcttttacgg tcatgacaat tccaatattc accgggccgc ccacacattg
     1021 gcggcccgcg caacggaact ctacgagggt gggcgtgaaa aagtgcgaca gtttatcggc
     1081 gcagccgata ccaaggaaat tgtttttgtg cgcggcacca cagaggccgt taatttggtc
     1141 gcgcagacct atggacgctc atatatcggc cgcggggacg aaatcctttt gaccgtgatg
     1201 gaacatcacg ccaacatcgt gccctggcag attctggccg agcagaccgg agctctgctt
     1261 cgggtcgctc cgatcaatga tgccggcgaa ttaattctcg aacaatttgc tccgctgctg
     1321 aaccccccgc accaaattag tagcggtcac gcaagtctcg aacgccttgg ggacgatcaa
     1381 cccagtggag gcgatcattg gaatggctca tgctcaaggg gtaccggttc tggttgacgg
     1441 tgcgcagtcc gcgccgcatc tcccgatcaa cgtacgggct atggattgcg atttcttcgt
     1501 attttcagga cataaaattt tcgcgccgac cggaatcggc atactgtatg gcaaggctgc
     1561 tctactggaa aagatgaaag gtaaagtcct gccatgttcg acagagatct tctgcttaca
     1621 gctgttcgtt atgagcggga ggatttagtt gacggacaca acaaagaatc ggtctatttg
     1681 gtctagaagg tcgcgctatt tgacgaggtc atgagtgggt tactgggaag aatgaagaca
     1741 aatttgaggc gggtatctga tggccacgcg tcatgaagtt cgggtaggtc gagctcaagg
     1801 gcaacgggaa gtacgacagt acaggtcaag agctccctag attgcgatga aggtcgagac
     1861 gatttcagga tgaaccagta aaatatgtac gatcagcggt ttagcgatgg ccgttgctcg
     1921 agcatctcac atgcgacatt actcactatg gggcatggct gccgatgttg atgacatgtc
     1981 cgcagtgatg caggattgca tgagctcaac tttgcattgc catgagatgg ctgcatcgtt
     2041 gacacgtttg accacatccg ccggcttcac ggcgctgggg gtaatgtcca cgagcacatg
     2101 gtcagggatg cggttgaagt agacgccggt cacgcctgga atggcttgca agacgttgtt
     2161 catctcagct tccttactgt cgcacccgga tccgctgagc atcaggggaa gccgttcttt
     2221 ggtgggtggg tttgctgaaa ccggtaggga aacggaaagg cttgcgatca agagtacggt
     2281 cgagacaact cgccggtgca ggaatgtgtg catgcgaaga actcccagac aagaaagtct
     2341 atccaccttc ctaaaataca tgatacggga tcatattgtc gatcgttact tggcctgcac
     2401 caaaaaggct tttgagcggg cagtgccgcc gccggcgacg acatcaatga atgacatgcc
     2461 gggccggaca tcaggaactg tcgcttcgac ggtcgtgtcg ctcacgaact tgtagtcgat
     2521 ctgggtgggg cccgccgcac tgaatgccac gccgcggaag caatccctcg ttccgaaatt
     2581 ctctcccttg atggtgatcg tgtgtcccgg cttggcttcg tccggctcca cgctcaagat
     2641 ctttggtttc ctgctgctat cgcagacagg gtctttcgac acgtgctgtt cgtcgtggcc
     2701 cttgatcgat tctgtatcgt ggagtgtgta gcccgctccg tcgacgatca ctccctcttc
     2761 agcaaccgag tgaaagggaa aacaggtgaa tccaaacaat gagaccatta ggaagggcga
     2821 cagaaatttg ggagacatcg ggacctcctt cgatggagaa ttacttgttc ggcatcggaa
     2881 tatagatttc tccgataatc gtttgtccgg gaggagagag cgcaaatttt gcaaaagcct
     2941 caaccagggt gttcgattca tttttcgaaa gcagcaacag agggcggcga aggctgtagc
     3001 ggccgtcctt cacggtcggc acttccggct cgaccttatc caccggcaac aaacggacgg
     3061 cgacgccgcc tgagacgacg gagaggccgg tgctcaatga aatataggcg actgcggaga
     3121 gaggcggcag ggtgccgacg acggtcttca cgacggcctc atcaacgccg atcattttgg
     3181 tggaatctgg aattttgtcc gctatcccga gttgagtttc gaaggcttcc cgaacgttct
     3241 ggttacgcgg tctgtcgatc aggagaatct tagtctccgg tccacccaca tccgaccact
     3301 ccttgatttt tcctgaaaaa atgtcggcga cttgctgctt cgtcacctct ttagtgaaat
     3361 tcgataaatg gaccaaaatg ccgattccat cccaacctat ctgagtcgcc gctaaagccg
     3421 gatcttcgac cccggtgacg gcaatatggg cctgacctgt cttgaccgat tgcaatggtt
     3481 tgcaatcctc gtcccactgg atgtcgacat aggcacgggg attggctttt tcaaaggctc
     3541 gagccaaggc ctcgatgatc gtttgttccg gcccgttgcc gacgatgatc agatttccgg
     3601 ctgtttgagc gaaggtgccg gcatgtgaca cacagagaga cactccgacg agcagcccgg
     3661 ttacgaactg tcccacggtc attgagcggc ctgtccccac tggaggtcac gaccgggttg
     3721 gtgttgggtg ctcaagaggt cgatccttcc gttgacggag gctctgattc tgaggtgacg
     3781 tgcggcgaca gcatgttagg tctgatattc agtggcggca acttggcaaa gcgctgcagc
     3841 cgttcctcgt tcatggcctt ggctttcagc tcagaaaagc cgggcttacg atcaccgacg
     3901 acattggacg aaaacgaaga catgagtcga gttgcctggc gggcccggca aaatggacat
     3961 tccgtgtctt cgactctggc atacacggac tgattggcct cgaattgttt ctcgcactgt
     4021 ccacaacggt attcgtagag cggcacggaa ttggatcctt tctgtgtctc tattgcagag
     4081 atatcggttc aggacttgac aaggcgacct ctcccaagcg atcttgaccg gctcgtaacg
     4141 atgcgtttat tctagcaaca gacgcaacta aactgccagc tttataggag gggttcgatg
     4201 ccagtgctga ttcgacggta caaaggtaat aacggcatga tgcaggaaga acgaatcgac
     4261 gacgacgatc gaatcgagcg ttacatgagg ctcttcgaaa aggacgacgt caagaagttg
     4321 gaaaccggcg tgaaggtctt tattgagaaa gatgagtggc aactcctccc ctagccggta
     4381 ggaggtgagg ggaataggag ttagacggat cgtctgtacc ctcaccacaa gcacctcact
     4441 ggccccgcat gatttattgg gttcacattg cacgcgcgat tgaccgtgtg actctccacc
     4501 gagaccggtg ttcggaggtt ccatccaccg cgacttccag tgatttctgg gagggaggat
     4561 ggtttgacta tccggacaag gaacgggcgc tccgtgccat ggaacaggcc ggaaccaatg
     4621 atcagcggcg gtgcccgctc tgtaagccgt agaaatataa aagtgctgag ttctgggtgc
     4681 tgagttcaaa gtcaaagaaa tgggcgacta tgttcagcac tgagcactca aaactcagca
     4741 ctctttaatt catgccgcga cttgcccttt tactgactac ggtcatctgg ggtgccacat
     4801 ttcccgcgac caaagcagcg cttgagcaga tccctccgct ttctttctta ttgctccggt
     4861 tcctcctagg cacgctcctg gtgcttctgt ggtttgtggt ctcccgtcgg cgactgcacc
     4921 gtgatcgtgt cgtattagga gcgagcgcgc tcacgaccgt ctttctcttt ctcggatacc
     4981 tgttgcaaac ggtcgggctc cgccatacga gcgcctccaa ttcagccttt ctgacggcgt
     5041 tgtacgtgat ctttgtgccg atgattctca tgcggatcga cagtcgggta gtgtcggcga
     5101 ctgcgattgc ggtggtcggg ctctggctgt tggtcaagcc cgatacctct atgaatctgg
     5161 gagatctcat gacgctaggg tgtgccgtcg cgtttgccgg gcatatcgtt tgtcttgagc
     5221 ggttcacccg gcaggttgat gctccgtcac tatttgtatg gcagatgatg gcgatgacgc
     5281 tgttgttcct tccggctcca tggtgggaag agacgacgca gagcgacttt tcgcccacgg
     5341 ccgtcttact gataggtctt ggcgtcacgg gtattctggc cacgctggca tttgccgttc
     5401 agatatgggc gcagcgactg gtgcctgccc aacaggtggc gttattgttt gcgtccgaac
     5461 ctgcctacgc ggcctggttg tcctggtact ttcttggtga gacgttggat gtgcaaggat
     5521 ggatcgggag cgcgctgatc ttattggcgg tggcgatcgg ggcgttcggc gcctaatcgt
     5581 ttcattaagg aggaatcgca tggcgcagaa gttcggtaac ggacgatggg tttgcaaagg
     5641 atttttggat aatcgcgtcg atggaacggt tgtgggccag gttgtctttg ccgtgatagg
     5701 gccggtggat ttttatttac gcggcaattt taagtcggat atcgcaggag aggtgattcg
     5761 atttcataat agccggtttg aagatgacga tatggccgga caagtcatcg gcgacatggc
     5821 aaaccagcag gtcggaacgg tgaatctgat ttcactggat ccgcacccga atcttgagcc
     5881 gcatccctac gtcgagtggt ttacgctcag aaaggatcac tatcgcttcg agctgagtcc
     5941 cggtgatgcg tgggtgctgt ccgatgagga gcgaaagacg atcgatgggg aaagccgacg
     6001 cattcgggag accctggccg atcgagtaac cgaccagcca aggtccgacg acggttccga
     6061 gtgggtctag ggaggggtca gttcaagttg aggttgaggc gccggcagtt ccgctgcttt
     6121 tacagctgca tcaatcttct ttttgagtac gctgcggtct gaatccagct tggtttttcc
     6181 ctcgcgcaca taccctggga caccacggat atcccagacc aaccccatcc actcaagtcc
     6241 tttcaggcag taataggtca tatcgatttc ccaccaatag aaaccttgtc ttgtcgcggc
     6301 a
//
LOCUS       DATNDD010000117         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1534954, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000117 DATNDD010000000
VERSION     DATNDD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534954"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /gene="tssK"
                     /locus_tag="VN647_02875"
     CDS             complement(<1..1299)
                     /gene="tssK"
                     /locus_tag="VN647_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HWG96005.1"
                     /translation="MSMNRTVVWSEGMLLTPQHFQQWDRHLEGLIADRFRIHDPFAWG
                     ITRLDINHDGLANGRFALLKCTVVMPDGLIVDIPDEDAAPQTRVFAELFHPTMENLSV
                     YLGLPIDRSDGMTCQLGKESGARPPRFLAEHIEAVDTTTGDNPREVLVAKKNLRIFFG
                     GEETADTVSIKIAELVRMPTGTVGLKETHIPACLWMSASPFLLRLLRGLIELLVAKRA
                     TFSDAQRGVMELIGADLGKFALVAAISTHLPLLQHLSRIEHVHPESLYSALVRLAGQL
                     LMLVPPGESPDLPSYQHENLSATFEALDKRIRSIIEGVTPTRYVIIPLDSSGDNVWVG
                     RVPESRLFASAQFFVMASGDLPEDQVRNLVPHQVKIGSPSKLKEIVAAAMPGVKLYHT
                     PRPPTSLPMKIGTQYFRLDDRGVFWEDIKKSQVVALHLPES"
     gene            complement(1323..1850)
                     /gene="tssJ"
                     /locus_tag="VN647_02880"
     CDS             complement(1323..1850)
                     /gene="tssJ"
                     /locus_tag="VN647_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HWG96006.1"
                     /translation="MNASWLLILQKDICRSIRLGPGRSAATTCLLVTLALCIGCSSDT
                     EKWRVLLFPTSTLNQDDTGYSLPVLVRVYQLKGKDRFQQATFNDLWKSDKEVLGDDLV
                     DRKEITVQPGTKVELQIDLEVKRGATHLGVMALFRKHDVEGWRQLVEASSSALNPMTP
                     KVKLVVDKNLLKLAD"
     gene            complement(1780..>2876)
                     /locus_tag="VN647_02885"
     CDS             complement(1780..>2876)
                     /locus_tag="VN647_02885"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HWG96007.1"
                     /translation="RGVWSICDVGSTNGTILNGIKIAPQEERELHDGDRITVGPYQLF
                     FQSVAPSTTPGASRESAPPVVPSSGTPATDPERLRYVLQRAYADFDGSSGENIEGYLE
                     SVLRRALEGCDRGAVRSAVQSLRTVLYSQTGQAIDVRNEAPPPKVEPAPVEPLRQESK
                     KRFLGGDRAREVYEELAVSGSSLNPDHMAKQLTKVLQTVCAGLADAVRGRREFQKEFE
                     VEATRILAWKPNPIKHAEDAVDIATILLDPASGGLSDEEAITSLKEAFQDLTLHQLGL
                     MAGFRECIHGLLKELHPDVIGKVRPGESKGKAIGLLTGGNIRSEAAAWRRYVEKHRQL
                     TEEEVKVFERILAPHFTKGYLSVHKTRTRA"
BASE COUNT          629 a          845 c          749 g          653 t
ORIGIN      
        1 actctcagga aggtgcaacg ccacgacctg cgacttcttg atgtcctccc aaaacacacc
       61 tcgatcgtcc agacggaaat actgagtgcc gattttcatc ggtagggagg tgggaggacg
      121 cggcgtatgg tacagcttga cacccggcat agccgccgcc acgatttctt tgagcttgct
      181 gggggatccg atcttgacct gatgcggcac gaggttgcgg acctgatctt cgggtaggtc
      241 gcctgacgcc atgacgaaga actgcgcaga ggcgaagagt ctgctttccg gcacgcgacc
      301 gacccacaca ttgtcgccgc tgctgtccaa tggaatgatg acataccgtg tgggtgtgac
      361 gccctctata atggagcgaa tccgtttgtc cagggcttca aaggtcgcgc tcaggttttc
      421 gtgttggtat gaaggaagat ccggtgattc gccgggagga acgagcatca gcagttgacc
      481 ggccagccga accagcgccg aatacaggga ttccggatga acgtgctcaa tccggctcaa
      541 gtgctggagc aagggcaagt gtgttgagat cgcggcgacc agtgcgaact ttcccagatc
      601 tgcaccaatg agctccatca caccccgctg ggcatccgaa aaggtcgccc gcttggccac
      661 caataattca atcaatcccc tcagcaagcg caacaagaaa ggtgaggcac tcatccatag
      721 acaggctggt atatgcgttt ctttcagccc aacggtgcct gtcggcatgc ggaccaattc
      781 agcaatcttg attgaaaccg tgtcggctgt ttcctctcca ccaaagaaga tccgcaagtt
      841 ctttttcgcc accaacactt cgcgaggatt gtcacccgtg gtggtatcaa ccgcttcgat
      901 atgttcagcc agaaatcgtg gcggccgggc accggactcc ttcccaagtt gacaggtcat
      961 gccgtcggac cgatcgatag ggagccccaa gtagacgctg aggttctcca tggtcgggtg
     1021 aaacagctcc gcgaagaccc tcgtttgcgg agcagcgtct tcgtccggaa tatcaacaat
     1081 caacccatca ggcatcacga cggtgcactt caagagcgca aaccgtccgt tggcgagtcc
     1141 atcatggttg atatccaatc tggtgatgcc ccaggcaaac ggatcatgaa tgcgaaagcg
     1201 atccgcgata agcccttcga gatgccgatc ccactgctga aagtgctgcg gagtgagcag
     1261 catgccttcg ctccacacca cggttcgatt catggacatg gcctcaacgt cttccttcgc
     1321 cgttaatcag cgagcttaag caagtttttg tcgacgacca gtttgacttt gggagtcatg
     1381 ggattcaacg cggaggaact ggcttcgacg agctgcctcc agccttccac atcatgcttc
     1441 cggaagagcg ccatcactcc caggtgcgtc gctccgcgct tcacttcgag atcgatttgc
     1501 aactcaactt ttgtacccgg ttgaaccgta atttctttgc ggtccaccag atcgtctccc
     1561 agcacttcct tgtcactttt ccagagatca ttaaatgtgg cttgctgaaa cctgtccttc
     1621 cctttcaatt gatacacccg taccaaaacc ggaagggaat acccggtatc gtcttgattc
     1681 agtgtacttg tcggaaaaag taagacccgc catttttcgg tatcgctact gcaaccgatg
     1741 cagagcgcca aggtgacgag tagacaggtc gtggcggcgc tacgcccggg tccgagtctt
     1801 atggaccgac aaatatcctt ttgtaaaatg aggagccaag atgcgttcaa aaactttcac
     1861 ctcctcttcg gtcaactgcc gatgtttttc cacatagcgg cgccatgcgg cggcttcaga
     1921 tcgaatattc ccgccggtca gcagccctat ggccttccct ttcgattcac cgggccggac
     1981 tttcccaatg acgtcgggat gcaactcctt tagaagcccg tggatgcatt cacgaaatcc
     2041 cgccatcagc cccagctggt gcagcgtcaa atcctgaaat gcctctttca ggcttgtaat
     2101 tgcttcctca tccgatagcc cgccggatgc gggatcgagc aggatggttg caatatccac
     2161 tgcatcttcc gcatgtttga taggattcgg tttccacgcc aagatccggg tggcctcgac
     2221 ttcgaactcc ttctgaaact ctctcctgcc ccgcacggca tccgccaagc cggcacagac
     2281 ggtctgcagc accttggtaa gctgcttcgc catgtgatcc ggattgagag aagatccgga
     2341 cacagccaat tcctcgtaga cttctctcgc ccggtcgccg ccaagaaatc tcttcttgct
     2401 ttcctgccga agcggttcca caggcgcggg ctcaaccttt gggggaggtg cttcgttccg
     2461 cacgtcgatg gcctgaccag tctgactata aagaactgtc cgcagggact gcacggcgga
     2521 tcgtactgca cctcggtcgc aaccttcgag cgctcttcga aggacggact ccaaatagcc
     2581 ttcgatattt tcacccgacg aaccgtcaaa gtccgcgtag gctcgttgca agacatatcg
     2641 gagtcgttca ggatctgttg ccggtgtgcc cgacgacggc acgaccggtg gagcagattc
     2701 tcgacttgcg ccgggagtcg ttgatggtgc gacagattga aagaacaact gatatggacc
     2761 gaccgtgatc cgatcgccgt catgcagctc gcgctcctct tgcggggcaa tctttattcc
     2821 attgaggatc gttccgttgg tacttccgac atcgcaaata ctccagactc cccgcc
//
LOCUS       DATNDD010000118         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1789870, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000118 DATNDD010000000
VERSION     DATNDD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789870"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VN647_02890"
     CDS             complement(<1..611)
                     /locus_tag="VN647_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWG96008.1"
                     /translation="MKQLVLIALRRPYTFVVLSILIVVFGALAVSEMPTDVFPVIQIP
                     VSSVVWIYDNLMPQDVEGRITYVFERFLTQTVEGIKYIWSNSLFGNSIINIYLQDGVD
                     LGGSEADIAAIAQTSVKALPPDINAPMVMRLFPSQVPVATLQVTSDSHTPAELYNLSI
                     MRIRPLLVTIPGAILPHPYGGQDMQVMINLDQQKLLARHLTPA"
     gene            1353..>2272
                     /locus_tag="VN647_02895"
     CDS             1353..>2272
                     /locus_tag="VN647_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWG96009.1"
                     /translation="MEDKLDEVETFKKWFKFDGIMAWFIITGVVLVGLSIGIMTLLES
                     DIVNRAGAHNRQVAFLRIAEFMGDMIGKTGGIRNRTVLEELIQDVREVRTGIQRLSVF
                     EITPESSLLILTTDPKAAPQTLDLQERLEIQAGHSVMQLDESSVERAWRITAPIAIDG
                     KVVGALRGLFSVKEYDDLFTQEIELAKTIGIGVVLVTSLTFLLLIRVKIHQPVHRLLY
                     AMRNVEAGDLSGHAPITGPVEIQEVTTQFNRMLDRVREAGLEKDRLLEEIRHFNQTLQ
                     KRISEATDKLQRANLELVEARLAVEGSQRLA"
BASE COUNT          546 a          551 c          630 g          545 t
ORIGIN      
        1 tccgctggag tgaggtgccg tgcgagcaac ttctgttgat cgagattgat catcacctgc
       61 atgtcctggc ccccgtaggg atgcggcagg atcgcgcccg gtatcgtgac caagaggggc
      121 cggattcgca tgatgctgag gttgtagagc tccgccggtg tgtgggagtc tgaagtgact
      181 tgtaatgtgg ccaccggcac ttgagatgga aacaggcgca tgaccatggg cgcattgata
      241 tcgggcggca gcgccttgac ggaggtttgt gcgattgccg cgatatcagc ttcgcttcca
      301 cctaggtcaa ctccatcctg aagatagata ttgatgatgg agttgccgaa taacgagttg
      361 ctccatatat atttaatgcc ttcgaccgtc tgcgtcagga agcgctcgaa gacatatgtg
      421 atacgcccct cgacatcttg cggcatcagg ttgtcataga tccaaacgac ggagctgacg
      481 ggaatttgaa tgaccgggaa aacgtcggtc ggcatctcgg acactgccaa tgctccgaat
      541 accacgatca ggatggacag cacaacgaag gtgtatggcc tgcgcagtgc gataaggact
      601 agctgtttca tgggattaga ccccccatta cctctgtaat tttgtgtgtc tgatttttcg
      661 tggccccata tcgacttttc agtctttaga accacagaac caccacgcct gaatgaaagc
      721 ttagcaaggt gtctgcctgg cacgctgaat ggtgcagaag tgacagttcg ctacaaaaaa
      781 agcgtgtcat aataacacag tgtgccaaag gtagccgaat gactcgatgt attgctcaat
      841 gaacgggagt gtcttgctca atatcttagc ggttgtgctg acattttctc caaatcactg
      901 gtcgcctcat cccttgaact atgatcaaca acgtagaccg taggcagtcc ggtttcacag
      961 gtaagttttc acgcagtcac cgccacgtcc gaagcattaa atttaggatc gatcaatctc
     1021 aatatgtgcg cgttaaaaat gtgccgtgtg tgcgattacg ctctcttaac aggattttga
     1081 cataggaaat ggttcgccga cgatgaagtt attgtctaat cagtcaacaa taggtgattc
     1141 tccgccagga tcatgttttg caggctgtgt cattgtgaca ttgatcgaat gtgcagaata
     1201 ctgaagccgg aggcgagggt ttcgctagtt ctacagctat agcaggatca ctctctaaac
     1261 agacaacccg aagccatgct ccgttacttc tgcgttggaa gtgccgggag atattctgga
     1321 tgtcgtcgaa cctattacat gtatataaat tcatggagga caagcttgac gaagtcgaga
     1381 cgttcaagaa gtggttcaag ttcgacggta tcatggcttg gttcatcatc accggagtcg
     1441 tgctggtcgg actgtcgatc ggcatcatga cactccttga aagcgacatc gtcaatcggg
     1501 caggggcgca taatcgacaa gtcgcgtttc tccgcatcgc tgagtttatg ggcgacatga
     1561 tcggcaagac gggcgggatc cgaaatcgta cggttctgga agagcttatc caagatgttc
     1621 gggaggtccg taccgggata cagcgccttt cagtctttga aatcacgccg gaatcgagct
     1681 tgctcattct gaccaccgat ccaaaggcgg cgccccaaac gttggatcta caggaacggc
     1741 ttgagattca agccggacat tcagtcatgc agctcgatga atcatcggtc gagcgggctt
     1801 ggcgcattac ggccccgatt gcgatcgacg gcaaggtcgt cggcgcattg cgcgggctgt
     1861 tttcggtcaa ggagtatgat gatctcttca cgcaggaaat tgaactggcc aaaacaatcg
     1921 gcatcggcgt tgtcctcgtc acatcgttga cctttttgct gttgatccgg gtcaagatcc
     1981 atcagccggt tcaccgactc ttgtatgcca tgcggaatgt ggaggccggg gatctgtccg
     2041 gccatgcacc gatcacaggc cctgtcgaaa tccaagaggt gacgactcag ttcaatcgca
     2101 tgctcgatcg cgtgcgggaa gccggattgg aaaaggatcg actcctggag gaaattcggc
     2161 atttcaacca gacactgcag aagcggatct cggaagccac agataaactg cagcgagcga
     2221 atcttgaact ggtcgaggcg aggctcgcgg tggaaggcag tcagcgcttg gc
//
LOCUS       DATNDD010000119         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1635571, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000119 DATNDD010000000
VERSION     DATNDD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635571"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VN647_02900"
     CDS             complement(<1..735)
                     /locus_tag="VN647_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWG96010.1"
                     /translation="MLKSKVNAWYGWVPDRPDHRDKLYTAIARPPKTLPPKVDLHDGC
                     SPVENQGQLGSCTANALVGNLEFLQKKAGHNPTDLSRLFIYYNERAMEGTIGEDAGAM
                     IRDGVKSLFKLGVCTEKLWPYKIARFTKKPSPACYKQAGNHQVTSYHRIIGLQQMKQC
                     LAEGYPFVFGFAVYEGFESAQVAKTGQLNLPKPSENELGGHAVLAVGYDDQAERILVR
                     NSWGTDWGIQGYFTMPYDYISNDNLAD"
     gene            complement(1203..1493)
                     /locus_tag="VN647_02905"
     CDS             complement(1203..1493)
                     /locus_tag="VN647_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWG96011.1"
                     /translation="MAFQEALQRYRMTFRPNRPGTPHLNGKVGRSQQTDWVEFYSTVD
                     LEDPALPELREEWQFFYNWHRPHSALSGKIPMERCCDLLEMTPWQEKVENQY"
BASE COUNT          617 a          654 c          552 g          687 t
ORIGIN      
        1 gtccgcgagg ttgtcattgg agatgtagtc atagggcatc gtgaaatagc cctgaatgcc
       61 ccagtccgtt ccccaggaat tccgcacaag gatccgctca gcttgatcgt cataccccac
      121 cgctagcaca gcatggccac caagctcatt ttcgcttggt ttgggcagat tcaactggcc
      181 ggttttggcc acttgagcgg actcaaatcc ttcatagacc gcaaatccga atacgaaagg
      241 atagccttct gcaaggcatt gcttcatctg ctgcaagccg atgatccggt gataggaggt
      301 gacttggtga tttcccgcct gtttgtaaca agccggcgat ggtttcttgg tgaatctcgc
      361 aatcttgtat ggccacaatt tctcagtgca gacgcccaat ttgaacagcg atttgacacc
      421 gtcgcggatc atggccccgg catcctcgcc gatggtacct tccatggccc gctcgttgta
      481 gtagatgaac aggcggctca ggtcagtggg attgtgtccg gccttctttt gaagaaactc
      541 tagattccca accagcgcat tcgcagtaca gctgccgagt tgcccctgat tttctacggg
      601 cgagcaacca tcgtgcagat cgactttcgg cggcaatgtc tttggaggcc tagcgatggc
      661 ggtgtagagt ttgtctcgat gatcaggccg gtctggaacc cagccatacc aggcattcac
      721 ttttgatttc agcatcttct ccctcctttg cttagtgttc gttaaaacat cacgtcttgc
      781 ttttggctgc atgaaataac tgcacaacat aggttcttat tcatggccaa catttccgta
      841 tgctgaccga gcaactaaat cacagacagc aaacatcatt ccatgagaca acatgaaatt
      901 ttgttcgatc gagacggttc cccactggta caaacttttt ctgatcagat tctaagcgta
      961 atgttttatt taaattaatt cttctcgttc aaacgatggt tgtttctaga tccatgcatt
     1021 ttgtattaaa gtggtacacc gctagcagca gtgaaccgga atcctgtatc gaaacagatc
     1081 caaggaggtg tatctaaaac gatccggtgc agcattcatc tgtgaagata cagacatcct
     1141 tcaattctgc gagccgttga tcggttgcaa attgacgcac cttggctcgt tcctgcttga
     1201 agctagtact gattttccac cttttcctgc catggcgtca tctcgagaag atcacagcag
     1261 cgttccatcg gtatcttgcc gcttaaggcg ctgtgaggac gatgccagtt ataaaagaac
     1321 tgccactcct cgcgaagctc tggcagcgcc ggatcttcga gatctaccgt ggagtaaaac
     1381 tccacccaat cggtttgctg agaccgcccg accttgccgt tgagatgtgg ggttccaggt
     1441 cggttgggcc ggaacgtcat gcgataccgt tgcagggcct cttggaacgc catcccaaac
     1501 cactcgacct cgcgatctgt ctgaacgcat tggatcgagt cacttttgcg attttgatga
     1561 atccttagtg ctgctctcta cccagccatc gaatatcgtc ttcccacctt accgaattac
     1621 tgagcaaatc gttcccacgc ccgatccgct acgccaaacc tcagtctcgc caagtgatgg
     1681 cccaacttct catgactgta tcttcattga ttcaggtagt cagtatcctt ttagattcac
     1741 ctgtatcgaa gcaatacaca ccgagcaagt ttcaatgact aactttctga aaacttttaa
     1801 ttctttcgta ctgaggtcag aaagatacta cgcataaatt ttgcttttgt cccagcacgt
     1861 acaacatcca attggaagaa gtgcgccctt atgtataagc gcagaaccta ggaccgaaca
     1921 aaaggatcta gttcaaatgg ctctattcaa cgggccatgt atctactatt gtatgtgcca
     1981 ttaagaagcc gattttggct agatgtccct aattggacct gtctctgttt ggacggatct
     2041 ataccaaagc catattgcta gcgctattgc cccgaatact caagtgaact cacgcatctt
     2101 ttgcatgggc taatatgcgg caagggcata gacaaggtag accaactcct gaacaatccg
     2161 gcatctgtgt attccggaac gcgaatgcac atctgaaaac aaatgcgtaa tcattgtttt
     2221 ctgctgctca atcttaatgc ttcaagttgc cctaacaatt ctaccgaatt agcatccatt
     2281 ctccgtaatt accactggcc ctgggatgtt gagctatgcg tcaaagacct accattgaat
     2341 gcctcgactg tctctccaac actcattttc ttttgtacac aaactctctc ccagcataag
     2401 gatatcgttt gcatccttag gaagacctgg gaagcaactc cactgataag cgtatggtgc
     2461 ccgaacaatt ctctggtaga ggatctaaac agtaccgtct ggaatgatgt
//
LOCUS       DATNDD010000120         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_934321, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000120 DATNDD010000000
VERSION     DATNDD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934321"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1055
                     /locus_tag="VN647_02910"
     CDS             393..1055
                     /locus_tag="VN647_02910"
                     /inference="COORDINATES: protein motif:HMM:NF033857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSL0067 family protein"
                     /protein_id="HWG96012.1"
                     /translation="MEPVPYAKLGDPQSLNLYAYVGNNPLDGVDADGHYGPEFAIPDS
                     SWVSDDPGTQEQKAKEAAARQQAQRQQAQQHAGRQPDGSYRADLSSPEIAPLLDPNHK
                     PSKSDVVGNGECVTFCKRAAGLEDTITSQWIPGPKVAGNDIARGTAIATLGSGGYPQE
                     DGVAKNSAIYLGKGTNGSIWILDQYNHPAHPPHPREIFFNSNPNHDVSNNANSYYVIH
                     VR"
     gene            1064..1660
                     /locus_tag="VN647_02915"
     CDS             1064..1660
                     /locus_tag="VN647_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96013.1"
                     /translation="MNRLWIIFILAFTTCSALGQTPKSRGTPNSEEALVQSLYDLVVT
                     RHPIGIPDGDDKGVFMPYLSQKLLHKIDLFRACDADWLRRNPDPNLKPPFGVFESGIF
                     SGGDERSEPRTFQVESTQAEKDGSHRVYVKLTWGKPSMSPQLIWRVAVVIVHEKSSFA
                     VDDVIYLKDKDHDVEWSLSKSLSYQCDGPRWVGSDQKR"
     gene            2167..>2385
                     /locus_tag="VN647_02920"
     CDS             2167..>2385
                     /locus_tag="VN647_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96014.1"
                     /translation="MSLSEIEAQLPNGLHDAKINSVCRDLKAQSVDIHLDVLMGLPDD
                     APANQNAFRAGVLKFTGAKIVIVEEPSVE"
BASE COUNT          617 a          593 c          654 g          521 t
ORIGIN      
        1 tcctttacaa gaacctgctc ccacgtccgt gcgcaggttc tccctggcat ggtggcccgt
       61 tctttcattg ggagccgcca cgctcgcggc tgtcatcatc atggtcgtgc tcctgcacca
      121 cacacatcaa accacttcac cacggtcaca ccgatgacct ttgccctgaa cgattggctt
      181 gggacgaagc gtgtggaggc tagtgtggcc gggcagatca gttcggtctt tccgtccttg
      241 ccttacggtg accagttgcc cacaggcacc ggtaacacag aacaattttt caccggcaaa
      301 gaacgggact ccgaatcagg gctggattac ttcggggcta ggtactacgg atcaagcatg
      361 ggcaggttca tgagtctgac tggagcacga aaatggaacc tgtgccctat gccaagctcg
      421 gcgatccgca gagcctgaat ctgtatgcct atgttgggaa taatccttta gacggagtgg
      481 atgccgatgg ccactatggc ccagaatttg cgatccctga cagttcctgg gtaagcgatg
      541 atccaggtac tcaggagcaa aaggcaaagg aagccgcagc cagacagcaa gcccaacggc
      601 aacaggccca acaacatgct ggacgccagc ccgatggatc gtatagggct gacttgagta
      661 gtccggagat agctccactg ctagacccga atcataaacc gtcgaagtca gacgttgttg
      721 gcaatggtga atgtgtaaca ttttgcaaga gagctgcagg acttgaggac acaattacca
      781 gtcaatggat acccggacca aaggtggctg gcaacgatat cgcacgagga acagcaatcg
      841 ctacgttggg tagtggtggc tatccgcagg aagatggtgt tgcaaagaac tcagcaatct
      901 atctaggaaa aggaacaaat ggaagtattt ggattttgga tcaatataac catccagccc
      961 atccgcccca cccaagggaa atattcttca actccaatcc gaatcatgat gtctcgaaca
     1021 acgccaattc ttactatgtg atacatgtga ggtagcaaaa acaatgaatc gtttgtggat
     1081 catctttatc ctcgcgttta ccacgtgttc ggctcttgga caaacgccta agtccagagg
     1141 cacgccaaac tctgaagagg cattggttca aagcctttac gatctggtcg tcacgcggca
     1201 ccccatcggt ataccagacg gtgacgacaa gggtgttttc atgccttatc tcagccagaa
     1261 gctgcttcat aaaatagatt tatttcgtgc ttgcgatgcc gactggcttc gacggaatcc
     1321 cgatccgaac ttgaaaccac cattcggtgt gtttgagagc ggaattttct cgggtggtga
     1381 tgagcgatcc gaaccacgca ctttccaagt agagagcact caagcggaaa aggatggatc
     1441 tcatcgcgta tacgtgaagc tcacgtgggg aaagccgtcg atgagtcccc agttgatttg
     1501 gcgggttgca gtggtcatag tccacgaaaa gagcagcttt gctgttgacg atgtgatcta
     1561 cctgaaggat aaagatcatg atgtcgaatg gagtctctcg aagtctctct cttatcaatg
     1621 tgacggcccg cgttgggttg gcagtgatca gaaacgctga agtctgctaa agataggccc
     1681 aacaacagag tgggccagta cttccaagca acccgtctgg tctagggtca ggttggcaag
     1741 atgtaactcc gactcccgga ggcaaagtca atccgaagat tccgaagaga ttcagaggtc
     1801 caagaggaac cgaagtcgaa tttgatccgg gtgttgcagg caagccggga tggggcgaca
     1861 aagaacactg gcatgagata ggccccgatg gaaagcgcga agatggtcat ctcgcccctg
     1921 gtgctcctat ccccggaccg gatggtgttc cggagccacc ggctccagca gagagaagca
     1981 tgatggatcg gatgagatca attactcctg gtccgatctt gaagttggga acagcgggca
     2041 tcatcacgta catcatcatc gacgaagggt cgaggctgta tccgcccaga aatctaatcc
     2101 ctgttccgta gccaagagac gggggtaaaa cggttacttc ggcgtgaatg ttttgaagag
     2161 gaatttatga gtctgagcga aatagaagcg cagcttccaa acggtcttca cgatgcgaag
     2221 atcaactccg tctgtaggga cttgaaggcc cagtcggtag atatacacct agacgttctg
     2281 atgggtctgc ctgacgatgc accggcgaat cagaatgcgt tccgcgcagg ggtgctgaag
     2341 ttcaccggag ccaagatcgt catcgtcgaa gagccgagcg tagag
//
LOCUS       DATNDD010000121         5414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2809352, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000121 DATNDD010000000
VERSION     DATNDD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2809352"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2773
                     /gene="dnaE"
                     /locus_tag="VN647_02925"
     CDS             <1..2773
                     /gene="dnaE"
                     /locus_tag="VN647_02925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWG96015.1"
                     /translation="TVGDPNRMKFDTDQLYVKSTEEIAPAFAEFPGAMTNTCRIADHC
                     ELDLPLNKIYLPQYRVPDGFNDRESYLEHLALTGLKERIKERPSRIASAVYDQRLREE
                     LMVICSMGFAGYFLIVWDIIRFARSQGIPVGPGRGSAAGSLVAYALRITDLDPLIYTL
                     LFERFLNPERVSLPDIDMDFCMDRRGEVINYVVDKYGTDHVAQIITFGTLGAKAAIRD
                     VGRVLEMPYADADKVAKLVPNQLNITLQQALETEPRLRELVETDSKVKELMANAQSLE
                     GLARHASTHAAGVVISEGPLTDHVPLYKGANDEIVTQYSMGDVEKIGLVKFDFLGLKT
                     LTMIRRAATLINEMHLEAPPLVIDQVPFDDPATFALLSSGKTTGLFQLESSGMRDLLT
                     GLKPDRFEDIIAIIALYRPGPMDLIPDFIKRKQGKVPITYELPELEPLLQDTYGVIVY
                     QEQVMAIANKMAGFSLGQADILRRAMGKKKPEEMEKLRAKFLDGAKQKKIPEKKAEKL
                     YELIQKFAGYGFNKSHAAAYAVVCYQTAYLKAHYPTEFMAALMTTDMGNQDKLVGYFT
                     ECRDLGIKVLGPDVNASQKHFAVAGGAIRFGLAAIKNVGEGAVESVLEVRTQAGPFGS
                     FFDFCRRVDLHKVNKRMLEGLIKAGAFDSSGAKRAQLMAVLDQAVEEGAAAQRERELG
                     QISIFGEELNGHTVSPALPTPLLPSLAEWDQAQRLKYERELTGFYISAHPLARYEATL
                     SALSTTTTVRLKECSDGGEVKICGIIATVKSMLTKKGDRMAYLTVEDLQGTTEVIVFP
                     DLFRTAGELIAPERIVRITGTIDRGDKGTKIRGSKIEPLAEVQAQSIKRIRIRLTDRP
                     EVREQLPRLLDIFRRHPGSTSISMSFRTDGTLEAETAPLPHLTVCASDHFVSDVEEVL
                     GKGALSLLS"
     gene            2823..3794
                     /locus_tag="VN647_02930"
     CDS             2823..3794
                     /locus_tag="VN647_02930"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HWG96016.1"
                     /translation="MRDYLEFEKPIREIEEKIEKLSATATNGKSSSQNDIRKLRAKLA
                     QTEHELYKNLTPWQRTQLARHPQRPSTLDYITELTRDFLELHGDRVFGDDRAIVGGFA
                     RFNDRPVMIIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMKLAEKFNRPIITFIDTP
                     GAYPGIGAEERGQAEAIARNLCVMSRMTVPIISVVIGEGGSGGALALAVADRVLMLEH
                     SVYSVISPEGCAAILWDSPEKVPDAASALKMTARDLLDLGVIDTIVSEPLGGAHREPR
                     AVCDLIGKALTNQLFDLLDLSVEQLIAQRDQKYRKMGAVTGLLPVQA"
     gene            complement(3801..4394)
                     /locus_tag="VN647_02935"
     CDS             complement(3801..4394)
                     /locus_tag="VN647_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96017.1"
                     /translation="MSFTAAISIRLPPACETALLERFLKAEAMALWTVRSARLQDVPP
                     NVYAFLRKHEEDEQQHLAQFEAMIGRRPRERERLPSVPRQWPALAVQLYGYESLGLEF
                     ATLLVTLRPDLISIREDELVHVEFFEKEICRLLKDGTSAAEQARVSAHAWWRKLPRTV
                     DRYLEAAVLDPFRPVLAGRILSSIERRFTTTGLLKDG"
     gene            4564..5277
                     /locus_tag="VN647_02940"
     CDS             4564..5277
                     /locus_tag="VN647_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96018.1"
                     /translation="MTRPTGTMSLVAQRVVPAMLAVALLLEVGCVSRRTYEKVKAETL
                     EQTQALEAVREDVRELDREIAGLQASNRREDAAVSVLRADIQREEEQLPIMKQRAEDT
                     LASLKTQVATLMNQSWHLARKIVDIRQESVSLQTKVAQYKEEMERVRAPGMVASDRNK
                     AAITQAADSEVTPSSVASTQDAESTQVAQAALPPPGLAPVKPSVSAPPMNADPPQAND
                     SWLGIITGWFAKIWNWLFG"
BASE COUNT         1232 a         1525 c         1533 g         1124 t
ORIGIN      
        1 gacggtcggc gatcccaacc ggatgaagtt cgacacggat caattgtacg tcaaatcaac
       61 ggaagaaatc gctccggcat tcgcagaatt tcccggtgcc atgacgaaca cctgccgcat
      121 cgccgaccat tgcgagctcg acctcccgct gaacaagatc tatcttccgc agtatcgcgt
      181 ccccgatggg ttcaacgatc gtgagtctta tctggaacat ctcgccctca ccggactgaa
      241 ggaacgaata aaagaaaggc ccagccggat tgcttccgct gtctatgatc aacgccttcg
      301 ggaagaactg atggtcatct gttcgatggg atttgcaggc tactttctca tcgtctggga
      361 catcatccgc tttgcacgat cacaagggat cccggtgggg ccaggtcgag gttctgccgc
      421 cggcagcctt gtcgcctatg ccctgcgcat taccgacctc gatccgctca tctatacact
      481 cttgttcgag cgtttcttga atcctgagcg cgtctctctt cccgatatcg atatggattt
      541 ttgcatggat cgccgggggg aagtgatcaa ctatgtcgtg gacaagtacg gcacagacca
      601 tgtcgctcag attattacgt tcggcacgct cggggccaag gccgcgatcc gcgatgtagg
      661 gcgcgtgttg gaaatgccct acgccgatgc ggataaagtt gcgaagctgg tccccaatca
      721 attgaatatc acgttgcagc aggcattgga gacggagccg cgcttacgcg agctggtgga
      781 gacggactct aaagtcaaag aactcatggc caatgcgcaa tccctcgaag gcctggccag
      841 acatgcttcg acccatgcgg cgggcgtcgt gatctccgaa ggaccgctca ccgaccacgt
      901 gccgctctat aagggcgcca acgacgaaat cgtgacccag tactcgatgg gggacgtcga
      961 gaaaatcggg ttggtgaaat tcgactttct cgggctcaag acccttacga tgattcggcg
     1021 cgccgcgacc ctcatcaacg aaatgcatct tgaggcgccg ccgcttgtga tcgatcaggt
     1081 gccgttcgat gacccagcca cttttgccct cttgagctcc ggaaaaacaa ccgggctgtt
     1141 ccagctggaa agctccggta tgcgcgatct gctgaccgga ttgaaacccg accggttcga
     1201 agacatcatc gccattatcg cgctctatcg tccgggaccg atggatctca ttcctgattt
     1261 catcaagcga aagcagggca aggtgccgat tacctatgag ctgccggaac tggagccgct
     1321 cctccaggat acgtatggtg tgatcgtcta ccaggaacag gtgatggcga tcgccaacaa
     1381 gatggccggg ttctcgttgg gccaagccga tattcttcgt cgtgcgatgg ggaaaaagaa
     1441 gccggaggag atggaaaagt tgcgtgccaa gttcctcgat ggcgcgaagc agaagaagat
     1501 tcctgagaag aaagccgaga aactctatga gttgatccag aaattcgccg gctacggttt
     1561 taataaatcg cacgctgccg cctatgccgt cgtctgttac cagacggcct acttgaaagc
     1621 ccactacccg accgagttta tggctgccct catgacgacc gacatgggca accaagacaa
     1681 gctcgtcggc tatttcacgg aatgtcggga tctgggcatc aaagtcctgg ggcccgatgt
     1741 gaacgccagc caaaagcact tcgccgtggc cggcggggcg atcaggttcg gcttggcagc
     1801 cattaagaac gtgggcgagg gtgccgtgga gtcggttctg gaggtgcgga ctcaggccgg
     1861 ccccttcgga tcgttctttg acttctgccg acgggtcgat cttcacaaag taaacaagcg
     1921 gatgttggaa ggtctgatta aggccggtgc gttcgattcg agcggcgcca agcgcgcaca
     1981 actgatggcg gtgctcgatc aagccgtgga agagggcgcg gctgctcaac gcgagcgtga
     2041 gcttggacaa atcagcatct tcggagagga gctgaacggg cataccgtct cgccggcctt
     2101 accgactccg ctgctccctt cgcttgcgga atgggatcaa gcacaacgat tgaagtacga
     2161 acgggaactg accgggttct atatttccgc ccatcccctc gcccgctacg aagcgacgct
     2221 gagtgccttg tcgaccacaa cgaccgtgag attgaaggaa tgctccgacg gcggcgaagt
     2281 gaagatctgc ggcatcatcg ccacagtcaa atcgatgttg acgaagaagg gcgatcggat
     2341 ggcctatctc acagtggaag acctgcaagg gaccactgag gtgatcgtgt ttccagattt
     2401 gttcaggact gcgggcgagc tgatcgcacc ggaacggatc gtccgtatca ccggtaccat
     2461 tgatcgagga gacaagggca cgaaaattcg gggcagcaag atcgaacctc tggccgaggt
     2521 ccaggcgcag tccatcaaac gtatccgtat ccgcctcact gatcgcccgg aggtgcgtga
     2581 gcagttacct cgattgctgg acatctttcg gcggcatccc ggcagtacct cgatctcaat
     2641 gtcgtttcga accgatggta ccctggaagc cgagacggct cccctccctc atcttaccgt
     2701 ctgcgccagc gaccattttg tgtcggatgt tgaggaagtg ctaggcaaag gggcactctc
     2761 tttgctatct taggggcctc aggttgtgcc ttggctgatc gttgtacggg attgaacagg
     2821 ctatgcgcga ttatctcgaa tttgaaaagc cgatccgaga gatcgaagag aagatcgaga
     2881 aactttccgc cacggccacc aacgggaaat cgtcctccca aaacgacatt cggaagctcc
     2941 gtgcgaaact tgctcaaacc gagcacgaac tctacaaaaa tttgactccc tggcaacgaa
     3001 cgcagctggc ccgacatccg caacgtccaa gcaccttgga ctatatcact gagctgacga
     3061 gagactttct tgagctgcat ggcgatcgtg tgtttggaga tgatcgggcc atcgtgggag
     3121 gattcgcccg gttcaacgac cgccccgtga tgattatcgg tcatcaaaaa ggcaaaaccc
     3181 tcaaagagcg catgcaacga aacttcggca tgcccaaccc ggaagggtat cgcaaggcgc
     3241 tgcgcctcat gaagctggcg gaaaagttca accgtccgat catcaccttt atcgatacgc
     3301 ctggtgccta tcccggcatc ggtgccgagg agcgaggcca agcggaagcc atcgcccgca
     3361 atctgtgtgt catgtcccga atgacggtac cgattatttc cgtggtcatc ggtgaaggag
     3421 ggagtggagg cgccttagcc ctcgcggtcg ccgaccgcgt cctgatgctg gaacactcgg
     3481 tctattcggt catttcgcct gaaggctgtg ccgccattct ttgggacagt ccggagaaag
     3541 ttcccgatgc cgcctctgca ttgaaaatga ccgcacgcga tcttctcgat ctcggggtga
     3601 tcgacacaat tgtttccgaa ccgctgggcg gcgctcatcg cgagccgcga gccgtctgcg
     3661 acctgatcgg aaaggcactc accaaccaac tgttcgatct gcttgatttg tccgtcgagc
     3721 aactcattgc tcagcgggat cagaaatacc gaaagatggg ggccgtcacc ggccttctcc
     3781 ccgtccaagc ctgattgact tcatccgtcc ttcaacagtc ccgttgtggt gaatcgccgt
     3841 tcgatcgaag aaagaatcct gcctgccagc acagggcgga acggatcgag caccgccgct
     3901 tcgagatagc gatcgaccgt tcgcggtaat ttccgccacc acgcatgcgc ggagacccgg
     3961 gcttgctcgg cagcggaagt cccgtctttg agcagacgac aaatctcttt ctcaaagaat
     4021 tccacatgga caagttcgtc ctcgcggatc gagatcaagt ccggccgtaa tgtgacgagt
     4081 aacgtcgcga attcaagtcc cagtgactca tagccataga gctgaacggc cagcgcgggc
     4141 cattgtcgtg gcacggatgg aagccgttcc cgttcgcgag gccgacggcc gatcatggct
     4201 tcaaattgtg ccagatgctg ctgctcgtct tcctcgtgtt ttctgagaaa ggcatagacg
     4261 ttcggaggaa cgtcttgtaa acgcgccgac cgtacggtcc aaagcgccat ggcttcggct
     4321 ttaagaaacc gttccaatag agctgtttcg cacgccggtg ggagccgaat ggatatagcc
     4381 gcggtgaacg acatgatggc ctcatagtac gcaaactccg aggggttatc caatctctct
     4441 tgaaaggagg aatgaggagg gttgactgac actcatcatc tgtgtacatc ttacgaagct
     4501 caccggaccg actgttgacc cggcacaaga gtttcgggtt ggctaaccca tgaggcaaca
     4561 cctatgacac gtcctaccgg taccatgtct ctggtcgctc aacgggttgt tcccgcaatg
     4621 ctcgccgtgg ccttgctctt ggaagtcggc tgtgtcagcc gacgaacgta cgaaaaagtc
     4681 aaggcggaga cgttggaaca gacccaagcc ttagaagcgg tacgagagga cgtcagagag
     4741 ctggaccggg agattgcggg attgcaggct tcgaaccgac gtgaagacgc cgctgtgtcc
     4801 gtattgcgcg cagatattca gcgggaagaa gaacagttgc cgatcatgaa acagcgagcg
     4861 gaggataccc tcgcgtcgct caaaacacag gtggcgacct taatgaatca aagttggcat
     4921 ctggcgcgca agatagtgga tattcgacag gagagcgttt cattgcaaac caaggtggct
     4981 cagtacaagg aggaaatgga acgggtcaga gcgcctggga tggtggcttc cgataggaac
     5041 aaagccgcca ttacccaggc cgccgactct gaagtgactc catcttcagt agcgtcgact
     5101 caggacgccg aatcgacaca ggtcgcacaa gctgctctcc ctcctcccgg cctcgcgcct
     5161 gtcaaaccat ccgtctcggc acctcccatg aatgccgatc cgcctcaggc aaacgactcg
     5221 tggcttggaa tcatcaccgg ctggttcgcc aaaatctgga attggttatt cggataggac
     5281 gggttcttcg cacctgatcg aacgagagtc atatttctca tccacaatgt tcgtcggccc
     5341 attcggccgc tgcacggcct gtgagacgtc cggtcgccaa acaacccgtg agcagatatc
     5401 ctccggtcgg tgcc
//
LOCUS       DATNDD010000122         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1102332, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000122 DATNDD010000000
VERSION     DATNDD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102332"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VN647_02945"
     CDS             <1..395
                     /locus_tag="VN647_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG96019.1"
                     /translation="PAVEDHVQQVSRRTGLPIHLKILDVPNAVPLDQATCLFRVMQES
                     VQNVVKHAQATTVTVQLRGSSKGVGLSVIDNGKGFDPQDLLTHQQGLGLSSMEERLRQ
                     LYGFFRIQSRSSHGTKVCAWVPCEVEVA"
     gene            392..697
                     /locus_tag="VN647_02950"
     CDS             392..697
                     /locus_tag="VN647_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG96020.1"
                     /translation="MKRPRILMADDHAIVLAGLRKLVEAESEVVGMVEDGRSLVDAAQ
                     QLRPDIVLLDISMPLLNGLDAARQISKLVPESKLIFLTMHATPPMRPKLLRPEPPAI"
     gene            694..1044
                     /locus_tag="VN647_02955"
     CDS             694..1044
                     /locus_tag="VN647_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG96021.1"
                     /translation="MIKRSAAVELKQAIQAVMQGQHYMTPLITKDVLAATLQSAEDQP
                     SKPLVTHLTQRQREVLQLVAEGKGTKAIASILNISVKTVEFHKFRIMGELDLHSTAEL
                     IKYAIAEGLVSVSS"
     gene            1255..1461
                     /locus_tag="VN647_02960"
     CDS             1255..1461
                     /locus_tag="VN647_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96022.1"
                     /translation="MQVSAIIDLVYGALEEFGTTCSMEEVVGLCPDLTWNQVYLAIDY
                     LNKTGKVAVTLDPDRTYTVRVHPA"
     gene            complement(2240..3166)
                     /locus_tag="VN647_02965"
     CDS             complement(2240..3166)
                     /locus_tag="VN647_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HWG96023.1"
                     /translation="MRTKPGCKPVCFRRSNYGVVLAVSLIVLSGCAVQKGSTNSLSLN
                     IEQNAPPVLIADVSHKTIPSAISTHEVSSEEPLDPFAKPGEEGIEEYDPWEPLNAKFF
                     EFNRQLDRWVLKPVAKRYDAVVPNIVQVGISNIFYNSRVTPRFMNNIFQGKFKGAGIE
                     AGRFLINTTVGIGGFFDVAQRFNLTTPEEDTGQTLGFYGVKPGPYIMVPLLGPYTVRD
                     LVGYAGDIALNPIYWLVIPAMHNSNAIPTVISIDDRLATYLISFGTKTIEVVNDRSLN
                     LEKFQGVEESTLDLYAAVRNAYLQKRAKMIQE"
     gene            complement(3655..>3871)
                     /locus_tag="VN647_02970"
     CDS             complement(3655..>3871)
                     /locus_tag="VN647_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWG96024.1"
                     /translation="FKCNVRRIIDYPHLFGYLRDLYQTDGIADTVNFDHIKRHYYITH
                     DDINPSRIVPIGPDQDLSAPHGRDRLA"
BASE COUNT          929 a          962 c         1040 g          940 t
ORIGIN      
        1 gcccggcggt cgaagaccat gtccagcagg tatcccggcg cacgggctta cccattcatc
       61 tgaagattct tgatgttccg aatgcggtac cgctcgatca ggcgacctgt ctctttcgcg
      121 tcatgcagga aagcgtccaa aatgtggtga aacatgcgca ggcgacgact gtgacggtcc
      181 aactccgcgg gtcgtcgaaa ggggtcgggc tgtccgtcat tgacaatggg aaggggtttg
      241 acccacagga ccttcttacc catcaacagg gactgggatt gagcagtatg gaggaacggc
      301 tgcgacaact atacgggttc tttcggatcc aatctcggtc atcccatggc acaaaggtct
      361 gtgcttgggt gccttgcgag gtggaggtcg catgaaacgg ccccgaattc taatggccga
      421 tgatcatgcc attgtcctgg ccggacttcg gaagctggtg gaagccgaga gcgaagtggt
      481 cggtatggtg gaagacggac gatcactggt cgatgcggcc caacagttac gccccgacat
      541 tgtgttgctt gatatctcga tgccgttgct caatggatta gatgcagcgc gccagatcag
      601 taagctggtg ccggagagca agctgatctt cctgaccatg catgccaccc cacctatgcg
      661 accgaagctt ttaaggccgg agcctccggc tatttgataa aacggtccgc cgcagtggag
      721 ctcaagcaag ccatccaggc ggtgatgcag ggacaacact atatgacacc gctgatcacg
      781 aaagatgtct tggcggccac gctccaatcc gcggaggatc agcccagcaa gcctttggtg
      841 acccatctca cacagcggca acgagaggtg ttacagctcg tcgccgaggg gaaaggtacc
      901 aaggctattg cctcgatttt gaatatttcc gtgaagacgg tggaatttca caagtttcgc
      961 attatgggcg aactcgatct ccactcgacc gctgagttga tcaaatatgc gattgccgaa
     1021 ggcctcgtga gcgtgtcgtc ataagcgcgt tctggttttg catattgccc gaggtacaat
     1081 ctggactacc taatacaaca tcatgaaagc ccccgcacat ggatccccct tcagcggcac
     1141 tcgtaatctc ccctcatttt tctagggtct ttcctagttc acaagatctg tcctacttca
     1201 ttaccattaa aggatgggtt cgtataagtt ttacctcggt gcggggagaa cttaatgcag
     1261 gtttcagcga tcatagacct cgtctatggt gccttagagg agtttggtac cacttgctcc
     1321 atggaagaag tggttgggct ctgtccggac ctgacctgga accaagtgta tttagccatt
     1381 gattacctga ataagaccgg aaaagtcgcg gtgacgcttg acccagacag aacctatacg
     1441 gttcgggtcc atccagcgta ggccagacgg tgcagtactt acctagagaa gatcacgtgc
     1501 acagtatcgc ctagtcactt tacaagtcat accttctatc tatgtgacgt aagggatggt
     1561 cgttcagctt agggccgtag ggccgtggaa tgggaattca tatggaagct gagatcgggc
     1621 ttcatagcgt ggcgggaaac atttcttcac agtgatcgat caatttgcca aaaacatccg
     1681 tccaagctac ttgacagcat attttgtcag gaatagattc ggtggattgt cggttcccaa
     1741 aggtgccagg gaccgagttg ttgtctgaca tcagaccctg tggtgtcatt cttctttaca
     1801 tctttgtccg gggaatagtg tccattcggt ctcatccttg ggaggtgtgc tgtggtgttc
     1861 cacttcgttg cgtgacgggt ccgaacgaaa ggagtaacca tggatagtac cttcttaatg
     1921 tttgccgtag tgatgctcgt gatcttcatc ggcggcatca tcgtgtacaa gaagagctaa
     1981 ttatcgctct gcccatctac tatatcttgt aggcctggct cggcagtggt gaggctactc
     2041 aagaacaatt ctcacgtgtg ctgaaccaac ctataggtct gccttcttcc gagtggttcc
     2101 cgcgatatca gcgaaaccct tgtagttcca ataggttatc tctaccgaag ttactgagga
     2161 cagggccatt ccgcaggcga gagctgcggc gatctcaact tgactgcccg tcgaatcaag
     2221 tgacgacgaa tccgatgagc tattcttgaa tcatcttggc tcgtttttgc aggtaggcat
     2281 tccgcactgc ggcatagaga tccagagtag attcttcaac accttgaaac ttctccaaat
     2341 tcaatgatcg gtcgttcacg acttcgatcg tcttcgtgcc aaacgagatg aggtacgtag
     2401 ccagccggtc atctatagag atcacagtag gaattgcatt gctattatgc atggctggta
     2461 tgactagcca gtaaatcgga ttaagggcga tatctcctgc atacccgaca agatcccgaa
     2521 ccgtgtacgg ccccaaaagc ggcaccatga tataggggcc aggcttgact ccatagaatc
     2581 cgagcgtctg ccctgtatct tcttccggag ttgtgagatt gaatcgttga gccacatcga
     2641 agaatccccc gatcccaacc gtcgtattaa tgagaaatct cccggcttct attcccgctc
     2701 ctttgaattt tccctgaaaa atattgttca taaatcgcgg ggtcacacga ctattgtaga
     2761 agatattgct gatgccgact tggacgatat tcggaacgac ggcgtcgtat ctcttggcga
     2821 ccggcttcag cacccaccga tccagctgac ggttgaattc aaagaatttg gcattaagcg
     2881 gctcccaggg gtcatactcc tcgatcccct cttcgcccgg cttggcaaat ggatcgagcg
     2941 gttcttcaga agagacttcg tgagttgata tggctgaggg gatggtcttg tgagaaacat
     3001 ctgcaataag aacgggtgga gcgttttgct cgatgttgag gctgagcgag ttcgtcgagc
     3061 ctttttgaac ggcgcaaccg gagagtacaa tcaacgagac ggcaagaaca accccatagt
     3121 tacttcgcct gaagcagacc ggcttacacc caggttttgt cctcattctt aagataacct
     3181 cttgagatta ataaaacgaa catcgacaat gggcgccgca ctgtatcaaa agtcgcgtca
     3241 cccgccaatc attaatttct aatatggttt tttcgaagga gggggggcaa agatgtgtca
     3301 ctgttctccg tgagagaaca atcgtgtaca ctccgcacaa tgcgggaact gcaggacaga
     3361 gacgaagtca gtccatcggt tcagaggagt ctccagcacg aggaacgagg cgaggccgga
     3421 tttacagcaa agccgttcct cgcccactgt gggacgagga agatatcagt cggaaggagg
     3481 gttctctacg aatcaacagg atcacgggga aggttcccaa cgcataaaga tccggctcgc
     3541 cagcgtaccg gtaaccatca cgggccacct gatttctaga cggcggacta tgcactctgc
     3601 ttgtagacga ttatcccgcc aagaaggagt gcgcccatga ggaggcttac gttattaggc
     3661 cagacggtca cgcccatggg gcgccgacaa gtcttggtcg gggccgattg ggacgatacg
     3721 ggaaggattg atatcgtcgt gtgtgatgta gtaatgccgc ttgatatggt cgaagttcac
     3781 ggtatcggca ataccgtcgg tttggtagag atctctgaga tacccgaaga gatgtggata
     3841 gtcgatgatt cgtcggacat tgcacttaaa a
//
LOCUS       DATNDD010000123         3849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2088987, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000123 DATNDD010000000
VERSION     DATNDD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088987"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN647_02975"
     CDS             <1..270
                     /locus_tag="VN647_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96025.1"
                     /translation="DGYVKPLFRNVDVYDPEKDQDKTLSHKMYRAIIDGVMSLLENIP
                     RDEVATKGDVSGPVENPQASTWQIIEKLVQNAFFNAILPGFEERA"
     gene            267..1199
                     /locus_tag="VN647_02980"
     CDS             267..1199
                     /locus_tag="VN647_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWG96026.1"
                     /translation="MNRTSAESSLTSQSSAYNPWKLGGLSFYDLARRLWHESLQDEVL
                     GRAAQLSYYIVLALFPALLVLTALMGVFSVQSYMPNLMSYLRQVLPNDALSMVERFLK
                     QVATGSGANILSLGALGTLWASSSGMVAIMDALNVVYGVKQDSRPFWRARLTAIVLTI
                     GLAGFVILSLTLVLYGTRLSAWIADLVGLGGVFVVAWNLLQWPVVAAIMLFVVGVIYY
                     VCPDIRQDWRWVTPGSVFAVIMWFMVSLGFKLYVDNFGDYNKVYGSIAGIILLMLWFY
                     WSGMVLLLGGEINAEIEKAATARAHQDRDHEEAA"
     gene            complement(1312..1797)
                     /locus_tag="VN647_02985"
     CDS             complement(1312..1797)
                     /locus_tag="VN647_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWG96027.1"
                     /translation="MRLLLAVDDSENAHRAVRYVGSLLKRTPDVTVTLFHVMKPIPRV
                     LLEHGGSENPAIEEQLSARLREDRKNSIRKEREAECPVLKKACETLAQSGFDTSCVNL
                     KFGYEDNIAANILEEARSGLYGTIALGRRGMSRITRMFGGGVTDQLLRDAKGFAIWII
                     E"
     gene            complement(2005..2142)
                     /locus_tag="VN647_02990"
     CDS             complement(2005..2142)
                     /locus_tag="VN647_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWG96028.1"
                     /translation="MRILIVGAGVAGLTLAGLLHRQGHGLCIVDRQPEGADLGYALSL
                     W"
     gene            complement(2210..3316)
                     /locus_tag="VN647_02995"
     CDS             complement(2210..3316)
                     /locus_tag="VN647_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG96029.1"
                     /translation="MPACILISLMAVLLLYGCTHTAPFRDNQGRIVPGSIAAMETISI
                     GGIPQQLWFRGIDTHNPALLILHGGPGISEAALYRHFDSELEQHFLVVNWEQRGTGRS
                     FHTDIPSESMTIAQFLRDLDEVVELIRERFQKNNVVLLGESWGTALGTIYSVQHPEKV
                     GAYVGIGHAANMPHGERLSYDYALTQAMARSDEKAVKELTAIGSPPHTVDEMLASRRW
                     VERFGGSFHADLSTGKLIWAALNTDEVNLVDLILFGRGNRFSLEQLWPEFSQLDLTIY
                     KKFETPVFFLLGRSDWQVPAILAASYFKSIEAPCKQLIWFERSAHHPPFEQPEEFNRV
                     MIEDVLPAATGSTTFVKQSKSQDDSCERRSHVER"
     gene            complement(3497..>3849)
                     /locus_tag="VN647_03000"
     CDS             complement(3497..>3849)
                     /locus_tag="VN647_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWG96030.1"
                     /translation="FMTIELNHTIVPARDKEQSARFFADIFGLQYEGAAGHFAPVRVN
                     DRLTLDFDQDDSFDMHHYAFHVSDDEFDAIFDRVRQAELAYGSHPWDAENRKLNDWKR
                     RPRRLLPRSERSSV"
BASE COUNT          843 a         1023 c         1056 g          927 t
ORIGIN      
        1 gacggctacg tcaaaccgct gttccggaat gtcgatgtct atgatccgga aaaggaccaa
       61 gacaagacgc tgtcccacaa gatgtacagg gcgatcatcg atggcgtgat gtcgttattg
      121 gagaacattc cgcgcgacga agttgcgaca aaaggcgatg tgtcggggcc tgtcgagaat
      181 cctcaggcca gtacatggca gataatcgag aaactggttc agaatgcatt cttcaatgcc
      241 atccttccgg gattcgagga gcgcgcatga atcgtacttc tgccgaatca tcgctcacca
      301 gccaatcgtc cgcatacaat ccgtggaagt tgggagggct ctccttctat gacctggcgc
      361 ggcggctgtg gcacgagagt ttgcaggatg aagtgttggg acgggccgcg cagttgtcgt
      421 actacattgt gctggcgttg tttccagcat tattggtcct gacggcgctg atgggcgtct
      481 tctcagtgca aagttatatg ccaaacctga tgagctatct gcgccaggtc ttgccgaatg
      541 acgccctttc catggttgag cgatttttga agcaagtcgc gaccggcagc ggcgcaaata
      601 ttctctcgct cggcgcgttg ggcaccctct gggcttcgtc cagcggtatg gtcgcgatca
      661 tggatgcgct gaatgtggtg tacggcgtga aacaggatag ccgcccattt tggcgtgcgc
      721 gtctcaccgc gatcgtgctt actatcggac tagcggggtt cgtcatcctg tctctgacat
      781 tggtgttgta tgggactcgt ctcagcgcgt ggattgccga tctggtcgga ctgggaggag
      841 tctttgtcgt cgcatggaat ctgctgcaat ggcctgtcgt ggccgccatc atgctcttcg
      901 tggtcggcgt catttattac gtatgcccgg acatccgaca ggattggcgc tgggtcacgc
      961 ccggatcggt attcgccgtg atcatgtggt tcatggtttc attgggattt aaactctatg
     1021 tcgataactt cggcgactac aacaaggtct acggatcgat cgccggtatc atcctgctta
     1081 tgctctggtt ctattggagc ggcatggtcc tcttgctcgg cggcgagatc aacgctgaga
     1141 tagagaaagc ggccacagca cgggctcacc aggatcgtga ccatgaagaa gctgcctgat
     1201 gaggtttgtt catatgcaaa ggccatccct tggactattg caagacaacc aaccttgcca
     1261 cgcgaagatc gacggagttt ctacgcgcaa ctgcgacttc ttttgtgggg tttattcgat
     1321 gatccagatc gcaaacccct tcgcgtcgcg tagtaattga tcggttacgc cgccgccgaa
     1381 catccgtgtg atccgggaca tcccccggcg gcccaacgca atcgtgccgt aaagtccgct
     1441 tcgcgcctct tcgagaatat ttgcggcgat attgtcctca tacccgaact tcaaattcac
     1501 gcagcttgtg tcaaaaccgg attgcgccag cgtttcacag gccttcttca ggaccgggca
     1561 ttcggcctcc cgttccttcc ggatcgagtt tttccgatct tcgcgcaggc gggcgctcaa
     1621 ttgttcttcg atagcaggat tctccgaccc cccatgctcc aatagaaccc gtgggatcgg
     1681 tttcatcaca tgaaagagtg tgacggtgac gtccggagtg cgtttaagta gtgatccgac
     1741 gtaccggacg gcacgatggg cattttccga atcgtcgacg gccagtagga gacgcatctc
     1801 ggttcagacc tgacggaatc tcataaaaaa gctccgtcct ttttcaaggg cggagccttc
     1861 gaacatgcaa tttgggcatt atgcttggat aacgtttccg tcatgcattc acaatgtttc
     1921 cccttgcttg ttctagtcat acgatatcgc gttcctatgt cgtgcggtac tcggaaaatc
     1981 cctaaagtta gtgtggtgta ttagttacca taaactcaag gcatacccaa gatccgcacc
     2041 ctcaggctgt cgatctacga tgcacagtcc atggccttgg cggtgtaaca accccgcgag
     2101 tgtcagcccg gcaactccag caccgacgat gagaatacgc actgatgctc ctcgcgaatg
     2161 agacagcagg ggtaatcaat gctgaaattc ggcatcatct cacgtgggcc tacctttcca
     2221 catgggagcg ccgctcacag gaatcatcct ggctcttgga ttgcttgaca aaggttgtgg
     2281 atcccgttgc ggctggaagc acgtcctcaa tcatgactct attgaattct tcaggctgct
     2341 caaacggagg atgatgggcg gaacgttcga accagatcag ttgcttgcac ggagcttcga
     2401 tggacttgaa gtaagaggcg gcaagtatcg ccggtacttg ccaatcggag cgaccgagca
     2461 gaaaaaacac gggcgtctcg aacttcttgt agatggtgag atcaagctgc gagaactccg
     2521 gccagagctg ttccagcgag aagcgattgc cacgcccgaa cagaatcagg tcgacgagat
     2581 tgacttcatc ggtgttcagg gccgcccaaa tcagtttgcc ggtcgataga tcggcatgga
     2641 atgaaccacc gaatcgttct acccagcggc gtgaggccaa catttcatcg actgtgtgcg
     2701 gaggagatcc gatggcagtc agttccttga ccgctttctc atcgcttctc gccatcgcct
     2761 gtgtcagcgc atagtcgtat gagagacgct caccgtgcgg catattagcc gcatgtccga
     2821 tccccacata ggccccgact ttttcgggat gttgaacgga gtaaatcgtg ccgagggcgg
     2881 tcccccagga ttcgccgagc aggacgacgt tgtttttctg gaatcgttct cgaatcaact
     2941 cgacgacttc atccaggtcg cggagaaact gagcgatcgt catggactcc gaaggaatat
     3001 cggtatggaa agagcgtccc gtgcctcgct gttcccaatt caccaccaag aagtgctgtt
     3061 ccagctctga atcgaaatgg cgatagaggg ccgcttcact gatccctgga cccccgtgca
     3121 agatcagcaa agccggattg tgcgtatcga tgcctcggaa ccagagctgt tgtgggatcc
     3181 ctccgatgga gattgtctcc atggcggcaa tacttcctgg gacgatccgc ccttggttat
     3241 cccgaaaggg agcggtgtgg gtacaaccgt agaggaggag aacagccatc agtgaaatga
     3301 gtatgcatgc cggcattgaa tgccccatcg ctgagcaaca gtcgaagttc tcatgtgctc
     3361 gcggcagtca agtcactctg tctacaatgt tttccgccga ggatggatac cgttccatac
     3421 ttgagagctc gagccggtga tttgcattac cttcattttc cggcgggcat cgatggttta
     3481 cggcctggtg agcaattcaa acagatgacc gttcggatcg aggaagtaga cgcctcggcc
     3541 gccgtttcca atcatttaac tttcgattct ccgcatccca tggatggctg ccgtatgcca
     3601 attcggcctg tcgaactcgg tcaaaaatgg catcgaattc gtcatcgctc acgtgaaacg
     3661 catagtgatg catgtcgaat gaatcatcct ggtcgaaatc gagcgtgagt ctgtcattca
     3721 cccttacagg cgcaaaatgt ccggccgcgc cttcatactg aagcccgaag atatcggcaa
     3781 aaaagcgcgc tgactgttcc ttgtcacgcg caggcacaat tgtatggttg agttcgatgg
     3841 tcatgaata
//
LOCUS       DATNDD010000124         9108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_364676, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000124 DATNDD010000000
VERSION     DATNDD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364676"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VN647_03005"
     CDS             <1..241
                     /locus_tag="VN647_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96031.1"
                     /translation="RDDGERDKFADFDVAIRRANVSEDVPWTFGARGQLGYDEDNQGV
                     QEQDIVFWYVAHLPHMAALGQTKWLALGPTLRVQR"
     gene            complement(1170..1700)
                     /locus_tag="VN647_03010"
     CDS             complement(1170..1700)
                     /locus_tag="VN647_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96032.1"
                     /translation="MRVIHTIHTSTLCLLFITVVVTAAGAGDKGRSTSQTTALDKKTE
                     DRARYVIKIAGCNDCHTTGYAEAAGKIPEKDWLKGDAMGWRGPWGTTYASNLRLYMQK
                     LSEAQWIKVSRSVEFRPPMPWFVLREMTEQDLRAIYRFIRNLGPAGDPAPTYLPPGQE
                     PKEPFILFPSNPEASK"
     gene            complement(1776..1949)
                     /locus_tag="VN647_03015"
     CDS             complement(1776..1949)
                     /locus_tag="VN647_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96033.1"
                     /translation="MRMFQVRQTVTYELTTAAGTEQEAILKAKGIPLAQWTAEADEVT
                     AEMIDETDEIDEY"
     gene            2189..5146
                     /locus_tag="VN647_03020"
     CDS             2189..5146
                     /locus_tag="VN647_03020"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HWG96034.1"
                     /translation="MNAWLFDDTFTCSPFPLCIEGLPGEWGGGDSVAEEEELPLPPQN
                     VQAKPGNGRITVTWDPVPDAMYYNLYFQTSKGVQIKFSELTRPIAGPEDFKSVIGVKK
                     DKATCLEGASSPYVHDDLANGTCYHYAVTVVTPKGESPESQEVMAIPSPYLLAMVIGC
                     EGVNDGELNSPTGIALDKDGNIYVADTDNHSIQKFDKAGKFLARWGGEPSSQEGQFYY
                     PRGLAVGPDDAVYVADSGNNRVQKFDSEGNAQKAWGKFGFAWRGADMGRFDVPWGITT
                     DQDGNVYVSDTSNSRIQKFQADGQPLLKWGRDGSFDGAFFFPRGVAVDFVGNIYVADE
                     SNSRIQKFDARGSFLTKWGREGQGPGQFKSPWGIACDALGNVYVVDSGNHRIQKFDGN
                     GTFLCSFGNRGKTEGQVNFPYGIAVDKEGCVYVVDSGNHRILKYVPTEEEMNRGKDQT
                     KQTVETGGGKSPRSLAVKAGDTEVFLSWTEVSGAQSYNLYFSTASHVTLEGATKIEGV
                     TNPYTHEGLTNDTPYYYAVTASFEDGTESGLSGEVTATPVLIDITAPQNPYAVINHGA
                     FMTNSPDVVVTISATDLDTGVGAYFISESPLTPMAGTPGWVDATPAIKFGSTIPFILS
                     PGDGHKTIYVWFKDIGNNVSTPASATILVNTSGYLCVSKWGKPGRGASLLHGGEFMAP
                     MYGLCVDQQGSLFVVDNGNNRVQKFDHAGTFIILWGSFGSANSNFHNPTGIACDGKGD
                     VWVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNAPWGIAVDRVRGYVYVVDSANF
                     RVQKFDMSGEFIMAWGSFGNGDGQFYFPRGVAVDQEDGAVYIVDMGNHRIQKFDTSTN
                     VLPQLLTKWGGSSTAGHASSSLAQEAGQLRSPWGLSIDGAGNVYVTDTGNHRIEKFDR
                     EGNFITQWGGFGNGDGQFNFPYGIAVDAKGSVFVVDSGNTRVQQFMPAEEGSERLQDE
                     AEELTELEKAQGT"
     gene            5241..6707
                     /gene="guaB"
                     /locus_tag="VN647_03025"
     CDS             5241..6707
                     /gene="guaB"
                     /locus_tag="VN647_03025"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWG96035.1"
                     /translation="MLDKEPRLGLTYDDVVLVPTKSLIVPNEVDTGTFVSRNIRINIP
                     LVSAAMDTVTESRLAIAMAREGGIGIIHRVLSPGDQAMEVDKVKKSESGMILDPVTIS
                     PDETIRDAHQLMAKYRISGIPVTKGRKLVGILTNRDLRFETRMDMKVSQVMKRDRLIT
                     APEGTSLEKAREILHEHRIEKLPVVNKQFELKGLITIKDIEKRIKYPNACKDSHGRLR
                     VGAAVGVGRDTEERVTLLKKSGVDLVVIDTAHGHSQAVLDRAKMIKKLYPDLELIAGN
                     IGTAEAAKDLLKVGVDAVKVGVGPGSICTTRIVSGAGMPQLTAIADCAKVLNGSGVPV
                     IADGGIKFSGDITKALAAGASSVMLGGLFAGTEESPGETVLYQARTYKVYRGMGSIGA
                     MERGGGDRYGQGERPAQKLVPEGIEGRVPYKGSLAAVVYQLVGGVRSGMGYCGCKTIA
                     DLQRNATFIRQSVAGLRESHVHDVIITKEAPNYRMDWE"
     gene            6806..8362
                     /gene="guaA"
                     /locus_tag="VN647_03030"
     CDS             6806..8362
                     /gene="guaA"
                     /locus_tag="VN647_03030"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWG96036.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSPILPCTVPLATI
                     LAYRPQGIVLSGGPSSVYEKNAPRIAKEVFDQNIPILGICYGMQLVTHLSGGTVAKST
                     HREYGGADLTIDDAGDLFKGVGTDRSTAVWMSHGDRIERMPPGFRSIAHTNNSPVAAM
                     KRDDPKRRIYCLQFHPEVAHTPEGTKILRNFVYAICGCKPTWTMQSYVETAVGQIREQ
                     VGKERVICALSGGVDSSVAAALTHRAIGDQLTCIFVDNGVLRAGERDQVQKTFASQLH
                     LKLRILDGTKQFLADLKNVTDPERKRKIIGRRFIKHFEAESKKLKGIKYLVQGTLYPD
                     VIESISFKGPSATIKTHHNVGGLPARMKLKLIEPLRELFKDEVRVLGTELGLPAEIIW
                     RQPFPGPGLAIRVLGPVTPERLAILRAAEAIVDQEIRSADLYREIWQAFAVLLPIRTV
                     GVMGDQRTYENVIAIRAVTSVDGMTADWAKIPNEVLGRMSNRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            8902..>9108
                     /locus_tag="VN647_03035"
     CDS             8902..>9108
                     /locus_tag="VN647_03035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWG96037.1"
                     /translation="MDYRQEIDGLRALAVLPVILFHAGFQSFSGGFVGVDVFFVISGY
                     LITTIILAELEQGRFSIINFYERRA"
BASE COUNT         2109 a         2312 c         2682 g         2005 t
ORIGIN      
        1 aagggatgat ggagagcggg acaagttcgc cgattttgat gtggccattc ggcgtgccaa
       61 tgtgagcgaa gatgtccctt ggactttcgg agcacggggc cagcttgggt acgatgaaga
      121 caaccaagga gtccaggagc aggacattgt gttctggtat gtggctcacc tgcctcacat
      181 ggctgccttg ggtcagacca aatggttggc gctcggcccc accctccgtg tgcaacggta
      241 gtacgatgtg tacgtcccgg ctctcctgaa aactcgccgt taaccatgcg atccattcag
      301 tacagagcgg catatcttat gcgtgtacac acccgacgcg gcatgaattg ttttagttga
      361 catgagggat tgaagaggag taatggagga atagttcact gatgtgaggt gtctgcaatc
      421 ctctgaatct ttccgaagca aagagggggt tgagatggaa gaccttagtc caccggatca
      481 cgtcgccccg ccgagtcatg ggcgggcgtt gcgtcagtca cgctagccaa ttctccatcg
      541 gcagtcgccg tcccgcgtag ggaaccaagg cgtcgtatcg acaagccgtg gggagattgt
      601 cagtcagcgc gagaaacagg atcgccggca atccgaatct attcctgacg aacgcttcta
      661 agcccattcc tctcgcgggt cgtttatcac tacagatgag atctacatgg cgtcctaagc
      721 gccagacgcg gggcagccct tcctatggcg ggaagggttg cccgcagcgg ttgtgttaac
      781 cttttcagtg tgaatgagtc agcgataaca atgaaggttc atgacgccgg tggatcattt
      841 tacgggaaca gtaagaagat aatattatcg aggtcgaacg aaaggagcga gccatggata
      901 gtaccttctt gatgttcgcg tccgtcatgc ttgcaatctt catcggtggc ttagtcgtat
      961 acaaaaagag ctcgtaactg ctgtggggtc actatccggc cccacgtttg ggcaattatc
     1021 tgttggccaa aagcaccggt agcgagtgtc gagttttcgg taagggacaa cgccagggtg
     1081 tcagggttca ggtttgtcac taatgcgccg gtgcatggcg gctaagtttc atcggtctga
     1141 tgagaagcaa tgcttttctc gcccggcgcc tatttcgaag cctccggatt cgaagggaac
     1201 agaatgaaag gttctttcgg ttcttgaccc ggggggagat aagtgggtgc cgggtcgccg
     1261 gctggaccca agtttctgat gaaccgatag atggcgcgca ggtcctgctc ggtcatttcg
     1321 cgaagcacga accacggcat cggtggtcga aactcaaccg aacgtgagac cttgatccat
     1381 tgggcttccg aaagcttctg catatagagc cgtaagttac tcgcataggt ggtgccccac
     1441 ggaccgcgcc aacccatcgc atctcccttg agccagtctt tttcgggaat ttttcctgca
     1501 gcttccgcat agccggtcgt gtggcaatca ttacaccccg cgattttgat gacataccgc
     1561 gcccggtcct cggttttctt gtccaatgcc gtagtttgac tcgttgagcg tcctttgtct
     1621 ccggcaccgg ctgcagtaac caccacagtg atgaagagca aacacaatgt tgacgtatgg
     1681 attgtatgaa tcacgcgcat gttttcttct ccctgatgag tatcggcgaa tcgccatcag
     1741 tgccctcggg aaatggaata gcgaacgagc atcgctcagt attcgtcgat ttcatccgtc
     1801 tcatcgatca tctccgccgt gacctcgtcg gcctcggccg tccattgagc cagcggaatg
     1861 cctttggctt ttaaaatggc ctcctgctct gttcctgcgg ccgtcgtcag ttcatacgtt
     1921 acagtttgtc tgacttgaaa catcctcata attattcacc tttgaatcgt ctaaaatctg
     1981 atcacgggtt ttccatgggt aaccggcgca ttgtaatcga tccggctatg gataccatag
     2041 agcctcgtta atattctgtt tcagaagacc tctcgccgtt gccttgactc tatttccgtt
     2101 gctttgctag agttgtcagt ccaagtctaa acgattgaaa gttccactgt ttctgaattg
     2161 ttccacaagc aacctgaggc ataccgtgat gaacgcttgg ctttttgacg acacatttac
     2221 atgtagtccg ttccctctct gtatcgaagg actcccggga gagtggggtg gcggagattc
     2281 ggtggcggag gaagaagaac tgccgttgcc tccgcagaac gttcaagcca aaccaggcaa
     2341 tggacgcatt accgtcacgt gggatccggt tccggatgcc atgtactata atctctattt
     2401 tcagacgagc aagggtgtgc agatcaagtt ctcagagctg acccgtccca tcgccgggcc
     2461 cgaagatttc aagagcgtga tcggtgtaaa gaaagacaag gcgacctgcc tggaaggtgc
     2521 ctccagtccg tacgtccatg acgaccttgc caacggcacc tgttaccact atgccgttac
     2581 cgtcgtcact ccaaagggcg aaagtcctga atcacaagaa gtgatggcca ttccatcgcc
     2641 ctatcttctt gccatggtca tcggttgtga aggagtgaat gacggtgagt tgaactcgcc
     2701 cacggggatc gcgctcgaca aggacggcaa catctacgtg gctgataccg ataaccattc
     2761 gatccaaaaa ttcgataagg ctggaaaatt cttggctcga tggggcggtg aacccagttc
     2821 acaggaaggc cagttttact atccccgcgg gttggctgtc ggaccggacg atgccgtgta
     2881 cgtggccgac agcggaaaca accgtgtaca gaagttcgac tcggaaggca acgcgcagaa
     2941 agcgtgggga aaattcgggt tcgcctggcg tggagccgac atgggtcgat tcgacgtgcc
     3001 ctggggcatt acgacggatc aagacggcaa tgtatatgtc tccgacacga gcaactcgcg
     3061 cattcaaaaa ttccaagccg acggccagcc gctgttgaag tggggtcgtg acggcagctt
     3121 cgacggcgcc ttcttttttc cgcgcggcgt cgcagtggat ttcgtcggaa atatttatgt
     3181 cgccgacgaa agcaacagtc gtatccaaaa gttcgatgcc cgcgggagtt tcttgacgaa
     3241 gtgggggcga gagggccaag gacccggaca gttcaagtcg ccgtggggta ttgcctgtga
     3301 cgcattaggc aacgtctatg tcgttgacag cggcaaccat cgcattcaga agttcgacgg
     3361 caacggcacg ttcctttgct cattcgggaa ccgcggcaaa acggaggggc aagtcaattt
     3421 cccctacggc attgcggtgg ataaggaagg ctgtgtgtac gtcgtcgata gcggcaatca
     3481 ccgaattctc aaatatgtgc cgaccgaaga agagatgaat cgtgggaagg accagacgaa
     3541 gcaaacggtg gaaaccggcg gggggaagtc cccccgcagc cttgccgtca aggccggtga
     3601 cacggaagtc ttcctgagtt ggacggaggt gtccggcgcg caatcgtata atctctattt
     3661 cagcaccgca tcccatgtca cgcttgaggg ggcgacgaag atcgaaggtg tgacgaaccc
     3721 ctatacgcat gaaggtctaa ccaacgatac gccgtattat tatgccgtaa ccgcgtcgtt
     3781 cgaagacggg acggaaagcg gactgtcggg agaagtgacg gcgacgcccg tactcatcga
     3841 tatcacggcg ccacagaatc cctacgccgt aatcaatcat ggagccttca tgaccaattc
     3901 gcccgatgtc gtggtgacga tctcggcgac cgatttggac acgggggtcg gggcttattt
     3961 tatttctgaa agtccgctca cgccgatggc tgggacacct gggtgggtgg atgcgacgcc
     4021 ggcgatcaag ttcggatcca cgattccgtt catcctctcg cctggagacg ggcacaagac
     4081 catttatgtg tggttcaaag atatcggcaa caatgtctcc acgccggcca gcgccacaat
     4141 tttggtcaat acgtccggct atttatgcgt gtcgaaatgg gggaagcccg gacgcggcgc
     4201 ctcgctgttg cacggcggag agtttatggc gccgatgtac ggactgtgcg tcgaccagca
     4261 aggatcgctg ttcgtcgtcg acaacggcaa caatcgcgtg cagaagttcg accatgccgg
     4321 gaccttcatc attctgtggg gaagtttcgg ctcggccaat tcgaattttc acaatcccac
     4381 cggtattgcc tgcgacggca agggcgatgt ctgggtggtg gataccaaca accaccgggt
     4441 tcaaaagttc gatggaaagc tcggcggcta tctcatgaag ttcggttcgc gtggaaacgg
     4501 ggaaggacag tttaatgccc cttgggggat tgcggtcgac cgcgtacgcg gctatgtcta
     4561 tgtcgtcgat agcgccaatt tccgtgtaca gaaattcgac atgtccggtg agttcatcat
     4621 ggcctggggc agtttcggca acggggacgg gcagttctat tttccccgag gggtggcggt
     4681 cgatcaagag gacggggcgg tctatatcgt cgatatggga aatcaccgca tccagaaatt
     4741 cgacaccagc accaacgtcc tcccgcagct gttgacgaag tggggaggca gttcgaccgc
     4801 cgggcatgcc agcagttcct tggcacagga ggccggacaa ttgcgctcgc catggggcct
     4861 tagcatcgac ggagccggaa atgtctatgt gacggatacg gggaatcatc ggatcgaaaa
     4921 gttcgaccgg gagggcaatt tcatcaccca gtggggcggg ttcggcaacg gggacggaca
     4981 attcaatttt ccatatggaa tcgccgtcga tgcgaagggg agcgtctttg tcgtcgatag
     5041 cggcaacacg cgggtgcagc agttcatgcc ggcggaagag ggcagcgagc ggttgcagga
     5101 cgaggcggag gagctgaccg agttggagaa agcgcaagga acgtagaacg tttaagtagg
     5161 atggcaacga cgtccaagtc cggccaagca ggagacagaa gttccagatc atctcctatg
     5221 agcctgctag aaggggaacg atgcttgaca aagaaccgcg attaggactg acgtacgacg
     5281 acgtggtcct cgtgccgacc aagtcgctga ttgtgcctaa cgaagtcgat accggcacct
     5341 tcgtctcgcg caatatccga atcaatatcc ctctcgtcag cgcagccatg gatactgtga
     5401 cggagtctcg gctggcgatc gcgatggcgc gcgaaggagg aatcgggatc attcatcgcg
     5461 tcctgtctcc gggggatcag gcgatggagg tggacaaagt caagaagtcc gaaagcggca
     5521 tgatcctcga tcccgtcacg atttctcccg acgaaacgat ccgtgatgcg caccaactga
     5581 tggcgaaata tcgaatctca ggcattcccg tcaccaaagg gcgcaagttg gttggaattc
     5641 tcaccaaccg cgatctgagg tttgaaacca ggatggacat gaaggtgtcg caggtgatga
     5701 agcgagaccg gttgatcacg gcgccggagg gcaccagcct ggaaaaggcc agagagattc
     5761 ttcacgagca ccggatcgaa aaattaccgg tggtgaacaa acagtttgaa ctcaagggcc
     5821 tcattaccat caaggatatc gaaaagcgga tcaaatatcc caatgcatgc aaggacagcc
     5881 atgggcgttt gcgggtcggg gcggcagtcg gagtcgggcg ggatacggaa gagcgtgtga
     5941 ccctgctcaa gaaatccggc gtggatcttg tggtgatcga cacggcccac ggccattcgc
     6001 aagccgtgtt ggacagggcg aagatgatca aaaagttgta tccggatctc gagctgatcg
     6061 caggaaacat tggaaccgca gaagcggcca aagacctcct caaagtcggt gtcgatgcgg
     6121 tcaaggtcgg ggtcggaccg ggatcgatct gcacgacacg gattgtgtcg ggagccggga
     6181 tgccgcagct gaccgccatc gcggattgcg caaaagtttt gaatgggagc ggtgtgccgg
     6241 tcattgccga tggaggtatc aaattttccg gcgatatcac aaaggccttg gccgccgggg
     6301 cgtcttccgt gatgctcggc gggctctttg ccggaacgga agagtctccc ggtgagacgg
     6361 tgttgtatca agccagaacc tacaaggtct atcgcggtat gggttccatc ggggcaatgg
     6421 agcgaggagg cggggatcgg tacgggcaag gagaacgccc ggcgcagaag ctggttcccg
     6481 aagggatcga aggccgtgtt ccctataaag gctcgttggc cgccgtggtg tatcagttgg
     6541 tcggcggtgt gagatcgggg atgggatatt gcggctgtaa aacgattgcc gatttgcaac
     6601 ggaacgccac attcatccga cagtccgttg ccggcctccg tgagagccat gtgcatgacg
     6661 tgatcattac caaagaggcg ccgaactatc ggatggattg ggaatgattt tccgtgaggg
     6721 gttaggcgcc aggcgttagg cgagttctaa ctccgaagca agcatctgca cttcacgccg
     6781 caccccttac gcctcacggg attcgatgga actctggcac gatagaattc tggtccttga
     6841 ctttgggtcc cagtacacgc agctgatcgc ccgccgtatt cgcgaagcgc aggtctattc
     6901 gccaattctt ccctgtacgg tcccattagc gacgattctc gcctatcggc ctcaaggcat
     6961 tgtcctttcc ggcggcccgt ccagtgttta tgagaagaac gcgccgagga tcgccaaaga
     7021 ggtgttcgat cagaacattc cgatcctcgg tatttgctac ggcatgcagt tggtgaccca
     7081 tctttccggg gggacggtcg cgaagtcgac ccatcgcgag tatggcggag ccgatctgac
     7141 gattgatgat gccggggatc tctttaaagg agtcggaacc gatcggtcga ctgccgtgtg
     7201 gatgtctcac ggtgatcgta tcgaacggat gccgccggga tttcgatcga tcgcgcatac
     7261 gaataattcg cccgttgcgg cgatgaagcg ggacgatccc aagcgccgga tttattgtct
     7321 gcagtttcac cccgaagtgg cgcacacgcc ggaaggaacg aaaattctcc gcaatttcgt
     7381 ctatgcaatt tgcggttgca aaccgacctg gaccatgcag tcctacgtcg agaccgcggt
     7441 cgggcaaatc cgtgaacagg tcggcaagga gcgagtcatc tgcgcgctga gcggcggcgt
     7501 ggattcctcg gttgcggcgg cgctgacgca ccgggcgatc ggcgatcagc tcacgtgcat
     7561 cttcgtcgat aatggcgtct tgagggccgg tgaacgtgat caagtacaga agaccttcgc
     7621 ctcgcagttg catctcaagt tacgaatttt agacgggacc aaacagtttc tcgccgatct
     7681 gaagaacgtg acagatccgg agcgaaaacg aaagatcatc ggccgtcggt tcatcaagca
     7741 cttcgaggct gaatccaaga aactgaaagg catcaagtat ctcgtccaag gcacgctcta
     7801 ccccgacgtg atcgaaagca tcagcttcaa gggcccatcg gctaccatca agacgcatca
     7861 caacgtcggc ggattgccgg cgcgcatgaa gctcaaactt atcgagccgc ttcgcgaatt
     7921 attcaaggac gaagtgcgcg tgttgggaac ggagctggga ttgccggctg agatcatctg
     7981 gcggcaaccg ttcccgggtc ccggattggc gattcgcgtg ctgggcccag tcacaccgga
     8041 acggttggcg attctgcggg cagcggaagc gatcgtcgat caggaaatcc gaagcgccga
     8101 tctttatcgc gagatctggc aggcgtttgc cgtcttgttg ccgattcgaa ccgtcggagt
     8161 gatgggtgat cagcggacct acgagaatgt catcgcgatc cgagctgtca ccagtgtgga
     8221 cggcatgacc gccgattggg caaagatacc gaatgaggtt cttggccgca tgtcgaatcg
     8281 aatcatcaac gaagtcaaag gcgtgaatcg ggtggtgtac gacattagct caaagccgcc
     8341 gagtaccatt gagtgggagt agaatcttga agtaaattat gtagtcgaaa gctacatgta
     8401 aggatacgcg aagcagcatc aactcaaagc tgtagtttct cgaagtgacc gtctccagat
     8461 tgtagtgacg caagcgaggt gattattgtc ggttcactgg ttcaaatggg aatgagtaaa
     8521 ccggcggcgg cactgaacga gagctgcagc gcgagtaaat gcgtttgact acatcgaaag
     8581 ctgatatagg ggaacaattc ttactcagct agggaaggtc taaatagttc ccaatttctg
     8641 tcagtaggtg aacgaaactc cgggaatctg attgcttccc gttcagaact tcgaattaat
     8701 cagaccttct ctggaaccta tctccaaatt aacgttcgac gttccgaacg atcacggacc
     8761 caggcactga gatacaatac acgcattttc ataaaaatgc gatccttttc aatgcggaga
     8821 gcagaggtgc acgagaccga cctcctcaaa aatcgatttt gagataggtt ccagttgtga
     8881 aaacttgatg gagtctagtg aatggactat agacaagaga tagatgggct tagggcgctt
     8941 gctgtacttc cggtaatact gttccacgcc ggtttccagt cattcagcgg tggattcgtc
     9001 ggcgtcgatg tgttctttgt gatcagtggg tacttaatta cgaccatcat tcttgccgaa
     9061 ctggagcaag gcaggttctc gatcattaac ttttacgaac gaagagca
//
LOCUS       DATNDD010000125         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3803303, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000125 DATNDD010000000
VERSION     DATNDD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3803303"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VN647_03040"
     CDS             complement(<1..1030)
                     /locus_tag="VN647_03040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWG96038.1"
                     /translation="MTHSFPWLTVLILLPLIGAAAVFFVKDPSARLVALAVAVADLMI
                     SLPLWWLFDASSGNMQFMESALWIASPPIHYRLGLDGISLPLVLMTTVLMPLCILISW
                     HSIESRVRSFMAMLLIMEGAMIGVFSALDFVLFYVFWEAMLIPMYLLIGVWGGPNRLY
                     AAIKFFLYTLTGSVLLLVAILVLYFQGGHTFDILQLSQGTYSKSLQFWLFLAFFAAFA
                     VKVPMFPFHTWLPDAHVEAPTAGSVILASILLKMGTYGFVRFSLPMLPDASQAFTPLM
                     VVLSIIAIIYGAYMALAQPDLKKLIAYSSVSHMGFVTLGLFMFNIQGIEGAVMQMVNH
                     GITTGALFL"
     gene            complement(1193..>2545)
                     /locus_tag="VN647_03045"
     CDS             complement(1193..>2545)
                     /locus_tag="VN647_03045"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWG96039.1"
                     /translation="LVWYSFGSFNYHEIFPAAHEAGELTMNLLGPFGGTWEVSVFTVI
                     GLLLFAGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVARLAPIYNLS
                     PTAMSVVAVTGAATMVLGATIALTQTDIKRVVAYSTVSQLGYMIMACGLGAYASGMYH
                     LLTHGAFKALLFLGCGSVIIALHHEQDMRHMGGLKDKLPITYWTFVIGSLALAGFPLT
                     AGFFSKDDLLVSAWSSGDLGQILTLFGLLTALLTAFYSFRLVFVIFWGTSHIDPHHAE
                     HLHEPSQTITTPLIILACFSILTGYLGIPSFLEPVFSIGGDQPVHHGSAGPVIMITAT
                     LMGLIGIAAAYYVYVLNPDLPERLARQLGGLYRGSLNKWYVDEAYDRLFVRPTFAAAS
                     ELWKRVDVNLIDGAVNGVARGVAWGGWLLRLVQSGQTQHYALAMAAGIVVIVTIFLIS
                     "
BASE COUNT          650 a          694 c          759 g          442 t
ORIGIN      
        1 agagaaagag tgctcccgtg gtgatgccgt gattgaccat ctgcatcacg gcgccttcga
       61 ttccttgaat gttaaacatg aataggccga gcgtgacgaa ccccatgtgg ctcacgctcg
      121 aataggcgat gagctttttc aggtcgggct gcgccaacgc catatatgcg ccatagatga
      181 tggcgataat cgagaggacc accatcaagg gcgtaaacgc ttgagaggcg tccggcaaca
      241 tgggcaaact gaagcgcacg aatccatagg tgcccatctt gagcaggatg ctggcgagaa
      301 tcacgctgcc tgccgtcgga gcttccacgt gcgcgtccgg caaccaggta tggaagggga
      361 acatggggac cttgacggcg aatgcggcga agaacgccag gaacagccag aattgcaaag
      421 acttggaata ggtgccttgg ctcaattgaa ggatgtcgaa cgtatggcca ccctggaaat
      481 acagcaccaa aatcgcgacc agcagcagga cgctgccggt cagggtatag agaaagaatt
      541 taatcgccgc atagagacga ttcggtccgc cccagactcc gatcaagaga tacatcggga
      601 tcagcatcgc ttcccaaaat acatagaaca gcacaaaatc caaggctgag aacacgccga
      661 tcatggcgcc ttccatgatc agcagcatgg ccatgaagct ccggactctc gattcgatgg
      721 agtgccaaga aatcagaatg cagagcggca tgaggaccgt cgtcatgagg accagcggca
      781 gactgatgcc gtcgagaccc agccgataat gaatcggcgg cgacgcaatc cacagggcag
      841 attccatgaa ctgcatgttg ccggaggagg catcgaacag ccaccagagc gggagtgaga
      901 tcattaagtc agcgacggcg acggcgaggg cgacaagccg agccgagggg tccttcacga
      961 aaaagaccgc agctgctccg atcagcggga gaagaatcag taccgtaagc caaggaaagg
     1021 aatgggtcat cggtcaatag ccgtccgaga cctcgccacg ggcgggtcgt tagtcaatgg
     1081 tccatcttta atggttaccg gtcactagtc gatcgacgat ggaacgaaag agcagcgcca
     1141 tcgcgctctc ccatgacacc cgactaatga tcggtgaccg gtgacttttc tcctacgaaa
     1201 tcagaaagat cgtcacgatg acgacaatcc cggccgccat cgccagtgcg taatgttgag
     1261 tctgcccgct ctggaccagc ctcaacaacc atccgcccca cgcaacaccg cgcgcgacgc
     1321 cgttgaccgc tccatcgatg agattgacgt cgactcgctt ccagagctcg gacgcggctg
     1381 cgaatgtcgg tctaacgaac aggcggtcat aggcttcgtc gacataccat ttgttcaacg
     1441 aaccgcgata caggccgcct aactgtcggg ccaagcgctc cggaagatcc gggttaagca
     1501 catagacgta ataggccgcg gcaatgccga tcaaacccat gagcgtcgcc gtgatcataa
     1561 tgacgggtcc ggcggaacca tggtgaacgg gctggtcgcc gccgattgaa aagaccggct
     1621 ccagaaatga cgggataccg agataaccgg tcaaaatact gaagcatgcc agaatgatga
     1681 gtggtgtggt gatcgtttgc gagggttcgt ggagatgttc cgcatggtgc ggatcaatat
     1741 gggaggttcc ccagaagatt acgaatacga gcctgaagct gtagaaggcg gtcaggagag
     1801 ccgtcagcag accgaagagc gtcagtatct gaccaaggtc tcccgacgac caagcggaca
     1861 cgagaaggtc atccttgctg aagaagcccg ccgtcagggg gaagccggcg agcgccaggg
     1921 acccgatcac aaacgtccag taggtaatcg gcaacttgtc tttgaggccg cccatgtgtc
     1981 tcatgtcctg ttcatgatgc agcgcgataa tgaccgagcc gcagccaagg aacagcaggg
     2041 ctttgaatgc gccatgggtg agtagatggt acatgccgga ggcgtaggct ccgagcccgc
     2101 aagccatgat catgtaaccg agctggctga ccgtcgaata cgcgacgacc cgtttgatgt
     2161 cggtttgagt caacgcgatc gtggcgccga ggaccattgt tgccgcgcct gtcacagcga
     2221 ccacgctcat cgcggtcgga gaaaggttat agatcggggc gagacgcgcc accatgaaga
     2281 cgccggctgt gaccatcgtg gcggcgtgaa tgagggccga aatcggcgta ggtccttcca
     2341 tcgcgtcagg cagccagacg tgaagtggaa cttgtgccga tttacccacc gcgccggcga
     2401 acagcaagag tccgatgacc gtgaacacgg aaacttccca tgttcccccg aaaggaccaa
     2461 ggaggttcat cgtgagctct cccgcctcat gtgcggcggg gaagatctcg tgatagttga
     2521 acgacccaaa gctgtaccag acgag
//
LOCUS       DATNDD010000126         3741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2775468, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000126 DATNDD010000000
VERSION     DATNDD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775468"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VN647_03050"
     CDS             <1..855
                     /locus_tag="VN647_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWG96040.1"
                     /translation="LVARAIHYNSARSALPFVAVNCSAVPETLLESELFGHMKGSFTG
                     AISNKAGLFEIADGGTIFLDEIGDTTPTIQVKLLRVIQEREFRRVGGNQDIKVDVRVV
                     AATNKELERAVAEGSFREDLYYRLDVIPIRLPPLRMRTGDIPLLVNHFLERFSKESGK
                     PKPVISPEAMHVLLGHEWRGNVRELENLIERVVAFSTEGPVTDAEVRGWLHRPAAQPQ
                     QPAMPLDLTDEGLDLEGLINGIEKDLLLKALERSKWVKKKAARMLRLNTRSFRYRLEK
                     YAIKGGRD"
     gene            848..1762
                     /gene="ispE"
                     /locus_tag="VN647_03055"
     CDS             848..1762
                     /gene="ispE"
                     /locus_tag="VN647_03055"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWG96041.1"
                     /translation="MTNPASRSTSSPPFITVFAPAKINLVLRILDRRSDGYHNLWSLM
                     QTVQLEDELALSLSDNHSTITLRCDEPSLKTDPSNLVYRAAAAVLESSGRTVGLDMIL
                     TKRIPMGAGLGGGSSDAAATIVGLNRLLNLEWSRERMVHIGQTLGSDVPFFFFAPSAI
                     VEGRGETVAPVQIKGSRWVVLVNPGFPVETKWAYQQLSVSRTGVQPFSAVHAGLGKAS
                     ELSWEEVLQATGNDFETPLFRTHPVLHRIKQKLLAEGAGAALLSGSGATVFGVFHDET
                     TARRAEIGFRTEPHLKVYTVSTLSTLRQ"
     gene            1959..2900
                     /locus_tag="VN647_03060"
     CDS             1959..2900
                     /locus_tag="VN647_03060"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWG96042.1"
                     /translation="MNRELKIFSGNANLVLAQEISAYLGQKLGEATVSSFSDGEIRVK
                     IDENVRGADVFVVQSCCQPVNDSLMELLIIIDALKRSSANRITAVIPYFGYARQDRKD
                     QPRVPITAKLVADLITTAGADRVLSMDLHAGQIQGFFNVPVDHLYALPVLLEYIVKKK
                     ISDLVVVSPDAGGVERARAFAKRLQANLAIIDKRREGPNQAQIMNIIGDVQGKSVLLL
                     DDMIDTAGTIVQGAQACLDQGARDVITACTHAVLSGPALERLQASCLSQVVVTNTIPL
                     RGKELACPKLHQLSVAPLLGEAIRRIHEDESVSSLFA"
     gene            2952..3734
                     /locus_tag="VN647_03065"
     CDS             2952..3734
                     /locus_tag="VN647_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWG96043.1"
                     /translation="MKFDLTVAIREEAGKGAARSMRRLGKIPAVLYGQGECLLLTANP
                     EELVKILKSHAGSTALISLTVNGAQSKQNRTALLRDYQIDPVTGAVLHADLFEISMSK
                     PIRVKVPVKVIGGIPAGVKEGGVLHHNMRDMHVECLPAALLDHIEVDASALTIGSGIH
                     VKEIGAREGVRFLDDPDQMVVSVAAPMSDAKLEALLTSGAGAAGEPEVVAKGKEVGAE
                     GAAGAEPAKAGAAAPAGEAKAGEKKEAAAAPKAEKKEAEKKK"
BASE COUNT          869 a          937 c         1124 g          811 t
ORIGIN      
        1 ttggtcgcgc gcgcgattca ttacaacagc gccagaagtg ccctcccatt tgtggccgtg
       61 aattgcagcg cggtgccgga aactttgttg gagagcgagc tatttggcca catgaaggga
      121 tcgttcaccg gagcgatctc aaacaaggcg gggttgttcg agatcgccga cggagggacc
      181 atttttctcg atgagatcgg tgatacgacc ccgacgattc aagtgaagtt gctgcgtgtc
      241 atccaagaac gggagtttcg gcgagtgggc ggcaatcaag atatcaaagt cgacgtacgt
      301 gtggtggccg ccaccaataa ggaacttgag agagcagtcg cggaaggttc atttcgagaa
      361 gatctctact atcgtttgga tgtgatcccc attcggcttc cgccgttacg tatgcgcacg
      421 ggtgatatcc cattgctggt caatcacttt ctggaacggt tttcgaagga gagcggtaag
      481 cccaaacccg tgatcagccc ggaggcgatg catgttttgc tggggcacga atggcgcggc
      541 aatgtccgtg agttggagaa tctgatcgaa cgggtcgtcg cattctccac ggaagggccg
      601 gtgaccgacg ccgaagtgcg aggatggctt catcgacctg cggcacagcc tcaacaaccg
      661 gcgatgccgc tggatctgac tgacgagggc ttggacctcg aagggctcat caacggaatc
      721 gaaaaggatc tattgctgaa ggcgcttgaa cgatcgaagt gggtcaaaaa gaaagccgca
      781 cgaatgctcc gtctgaatac cagatcgttt cggtatcgtt tggagaagta tgctataaaa
      841 ggaggtcgtg actaaccctg cctcccgatc gacaagctca cctcctttca ttaccgtttt
      901 cgcacccgcc aagatcaacc ttgtccttcg gattctcgac cgtcggtctg acggttatca
      961 caacctctgg tctctgatgc agaccgtgca gttggaggac gagctcgcac tttcccttag
     1021 cgacaaccat tcgacgatca ccttgcgatg cgatgaacct tccttgaaga cggacccttc
     1081 caacctggtg tatcgcgctg cagcggcggt actcgaaagc agcggccgaa ctgttggatt
     1141 ggacatgatc ctcacaaagc gaattccaat gggagccggg ttgggaggcg gaagcagcga
     1201 tgcggcagcg acgatcgtcg gtctgaaccg gctattgaat ttggaatggt cgagagagag
     1261 gatggtccac atcggtcaaa ctctcggaag cgatgtcccg ttcttttttt tcgctccttc
     1321 tgcgatcgtg gaaggacgtg gcgaaacggt agcacctgtg caaatcaagg ggagtcggtg
     1381 ggttgtgctg gttaatccgg ggttcccggt cgaaacaaag tgggcgtacc agcagctttc
     1441 tgtaagccgc acaggagtac agccgttttc ggctgtgcat gcggggttgg ggaaagcatc
     1501 tgagctctca tgggaagagg tgctccaagc gaccggaaac gactttgaaa cgccgctgtt
     1561 cagaacgcat ccagtgctcc ataggatcaa gcaaaaactc ctggctgaag gggcgggggc
     1621 agctctattg tcagggagtg gtgcaacggt ctttggcgtg tttcatgacg agacgacagc
     1681 gcggcgggcc gagatcggtt ttcggactga accccatctc aaggtctata cagtttcaac
     1741 tctctctacc ctccggcagt aacgcataga cgttcgatgg tcatttgttg acagagcccc
     1801 gcgatttcct atacagtttc accccttgac tgactgctgt gtcagtggtg tgttcagcga
     1861 acgagagggt tggtttagaa aggggcaacg ctgctcatcg cataagaact agcagagaat
     1921 ccaatacgtt ataaaaagac acggttcagg gaagtccgat gaacagagaa ctgaaaattt
     1981 tctccgggaa tgccaatctt gtgcttgccc aggagatctc agcgtacttg gggcaaaaat
     2041 taggcgaggc gacggtctcg tctttcagcg atggggagat tcgagtcaag atcgacgaaa
     2101 atgtccgcgg cgccgacgta ttcgtcgtgc agtcctgttg tcaacccgtc aacgattcct
     2161 tgatggagtt gttgatcatc atcgatgcgt tgaaacgttc atccgccaac cgcattaccg
     2221 ccgtcattcc ctatttcggg tatgcccgtc aggatcgtaa agatcaaccg cgcgtcccca
     2281 tcactgccaa attggtcgcc gatctcatca ctactgccgg agctgatcgt gtgctttcga
     2341 tggatcttca cgccgggcag attcagggat ttttcaacgt gccggtcgat cacttgtatg
     2401 cactcccggt gttgttggag tacattgtta agaaaaaaat atcggatcta gttgtcgtct
     2461 cgcccgatgc aggtggagtc gaacgagccc gagcattcgc aaaacgtctt caagccaatc
     2521 tggccatcat cgacaaacgc cgcgaggggc cgaaccaagc gcaaattatg aacatcatcg
     2581 gggacgtgca ggggaaaagc gtgttgctcc tcgatgacat gatcgatacg gcgggcacca
     2641 ttgtccaagg ggcgcaggcc tgtcttgatc aaggcgctcg agacgtgatt acggcatgta
     2701 cccatgcggt gctgtcgggg ccggctctgg aacggttaca ggcgtcgtgt ctctcccaag
     2761 tggtggtgac caacacgatt ccgttgcgag ggaaagagtt ggcctgtccg aagttgcatc
     2821 aattgtcggt ggcgcctctg ctgggcgaag ccatcaggcg gatccatgaa gatgagtcgg
     2881 tgagttcact gttcgcctaa gccattatcg actgggtgct gcgtacgaag cagaggagac
     2941 ggaggaggat catgaaattc gatttaacag tggcaatcag agaagaagcg gggaaaggag
     3001 ccgcgcgatc tatgcggcgg ttgggaaaaa taccggccgt gctctacggc caaggggaat
     3061 gtctgttgct gactgccaat cctgaggaat tagtcaagat cctcaagtct catgcgggca
     3121 gcaccgcgct gatttcgctc acggtgaacg gagcacagtc caagcagaat cgcacagccc
     3181 tcttacgcga ctatcaaatc gatccggtga ccggggcggt cctgcacgcg gacctctttg
     3241 agatttccat gagcaagccg attcgggtga aggttccggt caaagtcatc ggcgggattc
     3301 ccgccggtgt caaggagggc ggcgtgctgc atcacaacat gcgcgatatg cacgtcgaat
     3361 gtcttccggc cgctctgctt gatcatatag aagtcgatgc gtcggctttg accatcggaa
     3421 gcggcattca tgtgaaagaa atcggagcac gcgaaggtgt ccgttttttg gatgatcctg
     3481 accagatggt agtcagtgtt gccgcgccaa tgtcggatgc caaactcgaa gcgctgctca
     3541 ccagcggtgc aggggcggcg ggtgagccag aagtcgtggc aaaaggcaag gaggttgggg
     3601 ctgaaggcgc agcaggtgcc gagcccgcca aagccggtgc tgcagcgcct gcgggtgaag
     3661 cgaaggccgg cgaaaagaaa gaagctgcag ccgctccaaa ggctgaaaag aaagaagctg
     3721 aaaagaagaa gtagcggtgc a
//
LOCUS       DATNDD010000127         5659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1222309, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000127 DATNDD010000000
VERSION     DATNDD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222309"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1372
                     /locus_tag="VN647_03070"
     CDS             293..1372
                     /locus_tag="VN647_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWG96044.1"
                     /translation="MPVYKPEGEKNYRIQFRIAGKTYVKSAKTGDKRVAERMEAEWKA
                     EIHGGLYLHNREPITVSQMFDNYLALPLAESTKRSARTFFKQFRKHTNCDVKAHEFNQ
                     GELEKYVVARRAAGKKESGIRTHILIFMGAWNRTDNKVYNVPALETPKLATPKYTTEY
                     LTPEQEEQLTHYLLTRRPHAAGTGHWQYEIHDLIFMYIDTGARYCEIARLEWDQVDLR
                     NKTIELWRNKTKSESFIYMTDRVHQILQRRAESKKHERWVFTNRSCTNHRRLSTTYLN
                     EAIRKAGINSTVHKLRHAYATKMLKAGMTLNDVRLLLGHSSIQTTQRYQHLESSDVSP
                     KAAAILNQQNVERNRSKLKIVGGKK"
     gene            1615..1941
                     /locus_tag="VN647_03075"
     CDS             1615..1941
                     /locus_tag="VN647_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96045.1"
                     /translation="MNEQPEPTQNSDDKLSSIRSDLEYIFIRLNLILDVCIVCHKALV
                     MQNAEQDGSVANVLQRCGGDMLHEQLEELTRVVEELGGTTDYSSEERIDEQVATILNG
                     EGGHDE"
     gene            complement(2291..2599)
                     /locus_tag="VN647_03080"
     CDS             complement(2291..2599)
                     /locus_tag="VN647_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96046.1"
                     /translation="MVNNRNVGRNAGGDDELISLPRIAANIVGDFVKGYEPRAGDDAT
                     VGTDVTAIGMIASNQAEPNARDATSFDDFGVPEKIPDSHQGKLFILRCPPLWRMVVSW
                     "
     gene            complement(2983..3915)
                     /locus_tag="VN647_03085"
     CDS             complement(2983..3915)
                     /locus_tag="VN647_03085"
                     /inference="COORDINATES: protein motif:HMM:NF037406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96047.1"
                     /translation="MEDEGGNDDVELAEAAAFGHKFVDLPGNIEHLITLEKEGAVYSH
                     FWDDAIGTFRYYRVPFLPAEIYQYVEAHVAKQQFDKALLAGATFDEALQSISNPEIAR
                     RVQSYGNYGRSKLRNGGEADLRGCAASFYTPELEALVCGQRRDRYQLLPDMKGVDKSA
                     LLLQMIDYFPTLARFMARRGNDRPPFLIQNEYDIQHLLFVAVRSVFDDARLEEWTSKH
                     AGKSKRVDLVVPSAQTLIETKIARSIAHARKLSDELKVDIESYHVHPVCKRLFMLVYD
                     PDHHITDPAVLSNELSGPRVKGSSAFDVQVLVRS"
     gene            complement(5099..5407)
                     /locus_tag="VN647_03090"
     CDS             complement(5099..5407)
                     /locus_tag="VN647_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96048.1"
                     /translation="MGNKKRERKKKASEPELPISTDEPMVLKDSTATEGAIEECLDEI
                     NNFVMTLDQYPPNIVAIAMSVHLQTMLRTLIECDMCTPQQVREFIRELERDVFDKTDF
                     "
     gene            complement(5519..>5659)
                     /locus_tag="VN647_03095"
     CDS             complement(5519..>5659)
                     /locus_tag="VN647_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWG96049.1"
                     /translation="KELIGKIDHFVTQYNKKCQPFTWTATADSILQKLERLTSRISGT
                     GH"
BASE COUNT         1375 a         1578 c         1472 g         1234 t
ORIGIN      
        1 tcttgacagc ggccggattg gtcggtttga caggctcctt cccaggggcc ttggcttccg
       61 tgttggtctg cactacttca gcgttcatat ctcacctcgt tgtttgaact tctggggccg
      121 acatcggcca cccaacacgg ccagggtctc ttggaagttc gaacaagaca acggccattt
      181 catcgtcggc aggaagacat gaccgttttc tttgaaggaa ttcgacagga tagtccgcgg
      241 cgtaccgctg gtcattcaaa cacgaatgac caacgcacgt cgggagagcg tcatgccagt
      301 ctacaagcca gaaggagaaa agaactaccg catccagttt cgcatcgcag gaaagaccta
      361 cgtcaaatcg gcaaagaccg gcgacaaacg agtagcggag cgcatggagg cagaatggaa
      421 ggcggagata cacggagggc tatatctaca caacagggag ccgatcactg tcagtcagat
      481 gttcgacaac tacttggccc tccccttggc ggaatccacc aaacgcagtg ccagaacgtt
      541 cttcaagcaa ttccgcaagc acacgaactg cgacgtgaag gcccacgagt tcaatcaggg
      601 ggagctcgag aagtacgtcg tagctcgacg ggccgcgggc aagaaggaat ccggtatccg
      661 cacgcacatc ctgattttca tgggcgcgtg gaatcggacg gacaacaagg tgtacaacgt
      721 ccccgccctc gaaactccca agctggcaac accgaagtac acgacggagt atctgacacc
      781 ggagcaggag gagcagctaa cacattacct gctcacccgt cggccacatg cagcgggaac
      841 cggacattgg cagtatgaaa tccatgacct catattcatg tacatcgaca ccggtgcccg
      901 atactgcgaa atcgcccggc ttgaatggga tcaggttgac ctcaggaaca agacgattga
      961 gctatggcgt aacaagacaa aatcggaatc tttcatctac atgacagatc gtgtccatca
     1021 gatccttcag cgtcgagccg agagcaagaa acacgagcga tgggtgttca cgaataggag
     1081 ctgcacaaac caccggagac tgagtactac gtacctgaac gaagcaatac ggaaggccgg
     1141 aattaacagc acggttcaca agctgcggca cgcctatgca accaagatgc tcaaggcggg
     1201 catgacactg aatgatgtcc ggctactgct cggccattcc tcgatccaga caactcagcg
     1261 gtaccagcac ctcgaatcca gtgacgtgtc acccaaggca gcggcgatcc tgaatcagca
     1321 gaatgtcgaa cggaatcgct cgaagctcaa gatcgtcggc gggaagaaat agagagcaca
     1381 tcagtactcc cgtcattcct gttgacggct gattcctcca gcctcgacag tattcctcgg
     1441 ctccacgctt gtctgacctt cagaaggacg gacagactcg cggtggaatc tgaagcggtg
     1501 cccaccagtg cgtcaacacc ggcaggcacc taagcacaac gtaacctgat aacgaggtca
     1561 catcatgcct gtgtccaagt ctatcgcggt tgccttccgc atgggaggtc atccgtgaac
     1621 gagcaacccg agccaaccca aaacagcgac gacaagctta gctccatccg gagcgacctt
     1681 gaatacatct tcatccgtct gaacctcatc ctcgatgtct gcatcgtgtg tcacaaggct
     1741 ctcgtgatgc aaaatgcaga acaggacgga tccgtcgcca acgttctgca gcgctgcggc
     1801 ggcgacatgc tgcatgaaca gcttgaggag ctgacgagag tcgtcgagga gcttggtggc
     1861 accacggatt attccagcga agaacgaatc gacgagcagg ttgccaccat tctgaatggg
     1921 gagggcggcc acgatgagta agggacacta tgaaaaggcg agcgtagtca tcaaggcgct
     1981 cgtgcaaggc accgacccgg agaccggcga gccctttcct cctgactccg ttctcaaccg
     2041 ctcggacgtg ctgagggcgc tgctgaccgc ttctgtggcg attgaaaaca tgatgtcacg
     2101 tgcggcgcgt cgggctcagc tacccagcaa cgtaggtcgc tcgtgggaaa ggacgaggag
     2161 gacacactcg ttgccgcttt caagtccggc gactccatcg cggccattgc cgctcggcac
     2221 ggcagaaccg ttcgagccat cgaagccaga ttggagaaat tgggtttgat gagttcgtcg
     2281 ggtgataggt tcaccagctc accaccatcc gccacaacgg agggcagcga agaatgaaca
     2341 gcttaccctg atgggagtcg ggaatcttct cgggaacccc gaaatcatcg aaagaggtag
     2401 cgtcgcgtgc gttcggttct gcctgattgg aagcgatcat tccgattgca gtaacgtcgg
     2461 ttcccacagt agcgtcgtcg cctgcgcgtg gttcatagcc tttgacgaaa tcgcctacaa
     2521 tattggccgc aatgcgagga agggagatca gctcatcgtc accgcctgcg ttgcgcccca
     2581 catttcgatt gttgaccata gggccagccg tttggccggc gaattcatcg ttacgggttt
     2641 caagtatggg gcaaaggacg gaggaccagg cgcagtcggc acaacggact ctagaacgcc
     2701 aagtccgccg acacagtcgg cgacggaagc ggccccgatg acagtatgac catgttcgga
     2761 actgtgtaac gcaccaaggc cctcgtcgcg acggcggggg ccgttttttt ctcgtaccgt
     2821 cgttgccatc agcacaaatt tcagcggacg gtacggattg aatggactcc tcagataccc
     2881 aatcgcctca cacgcccact gacgagcaaa tggcgatccc tgtcgcagtc atagtgaatg
     2941 aattcttttt ggcacgctcg cagcccacaa gtcgggctcc tgtcatgatc gaaccaacac
     3001 ctgcacatcg aaagccgagc taccctttac tcgcggaccg gagagttcgt tggagagaac
     3061 ggcaggatct gtaatatggt gatcgggatc ataaactagc atgaagagac gcttacacac
     3121 cgggtgaacg tggtagcttt cgatatcgac cttcaattcg tcagagagct ttcgagcgtg
     3181 tgcgatactc ctggcaattt ttgtttcaat gagcgtttga gcggaaggaa ccaccagatc
     3241 gacacgcttc gatttgccgg cgtgctttga cgtccactct tcaaggcgag catcatcgaa
     3301 taccgaacgc actgcaacaa acagcaaatg ttgtatatca tactcgttct ggatcaggaa
     3361 cggcggccga tcgtttcccc gacgagccat gaagcgcgcc aacgttggga aatagtcgat
     3421 catctgcaac agaagggcag acttatcaac acctttcatg tcgggaagta actgataacg
     3481 atcgcgtctt tgcccgcaga cgagagcttc tagttcaggc gtatagaagc tggcagcaca
     3541 tccccgaaga tcggcctctc ccccattcct tagcttcgag cgaccgtaat ttccgtatga
     3601 ctgcacccgt cgagcgatct cgggattgga gattgactgg agagcctcat caaacgttgc
     3661 gccagcgagg agcgctttat caaattgctg tttcgcaacg tgggcttcaa cgtactggta
     3721 aatctcagct ggcaagaacg ggactcggta gtagcgaaag gtgccaatag catcgtccca
     3781 gaagtggctg taaaccgccc cctccttttc gagggtaatg agatgctcta tgtttcctgg
     3841 gagatccaca aacttgtgac cgaaagccgc tgcttcggcc aactcaacat cgtcatttcc
     3901 gccttcgtct tccatggacc cgatctctta ttcaagacat tgacagcatg gttcggaggg
     3961 ataccactac ccagattcct atgttgaggg gtcaggctgc actggcttcc gtttcgcaag
     4021 atttgcgtat gtgctcacct gaatatattt ttcaaagtct ccgacaacac actttatatg
     4081 agccgtgcct tgaaacgaca acagaacgca ttcactcgga tgtgcctcaa ggatcgcgat
     4141 gcttgaatga tcgagatcca ccccatcaag acgtagccac gctacaccgt gtccgcctac
     4201 gacggcatta cacttgtcaa cgtatcgccc caaaatcgct gatcccaggc cacctccgtt
     4261 tacaatttgc caactgatct cggagacagt gatcggaaag ggcaaaagat taattagcga
     4321 gaactccgcg tcgtagaccc ttggcatttg cgatcgcatt ttctggatcg acggcccgcc
     4381 attatatgca aacagcaata tccacttgcg acgtgtagat attggatagg ttgaccggag
     4441 gacaataccc ggagcccaac taatcagccg ggaaagccca gccctagcaa gggcccccca
     4501 tgcgtcaaac atgggaggtc actgtacgtc ccaaacccat tcagcctgag cgtcttgcgt
     4561 ctttgatgta ttcacaatct tcgttaacgc gggtccacga gaggttgatt gtctcacatc
     4621 cgagaccatg tgcgtccagg agggaggtag ctttagcggt ggattatcta ccgacctcct
     4681 tgccggtaga tttccggtag acgcccctac cgtcctcaac tctgcatttt tctgcagtca
     4741 gatcatccat gcgccggaac gtgcacgctt ccaccgatag gacacgaaat tcccgtgcct
     4801 tttagcccac aataaccgtc gggattcttc gcgagatact gctggtgata atatcccacg
     4861 ttcgttttca cccgcagaat gatggcttca aatcttgttc ttggtctgaa cagtagaaaa
     4921 atagaccagg aattagcgcc aatatagtag agacgagagc caataaaaaa gccgcaagtc
     4981 gtctctcgtc gtcgtgactt gcggcttctt catttccatc cctggaaagc gcccgaccgg
     5041 taatctattt atgcttccgc ttcttcacat ttgctcgctc ctccctgcct cgctccggtc
     5101 agaagtcggt cttgtcgaag acatcccgct ccagttcccg aatgaactcc cgcacctgtt
     5161 gtggcgtaca catgtcacac tcaattaacg tgcgaagcat cgtctgcaga tgaacactca
     5221 tcgcgatggc gacgatgttc ggcgggtact ggtcgagagt catcacaaaa ttattgatct
     5281 cgtccaggca ttcttctatc gcaccttcgg tggctgtcga gtccttgagt accatcggct
     5341 cgtcagtcga tatgggtaat tctggctcac tcgctttctt cttccgctcg cgcttcttgt
     5401 tccccatgca gcattcctat gttctggaaa gctacaccgt catcgagttc tttaggaaat
     5461 ggaacagggg ttctatctct gctggcgccc tcccgatcgg cgcttaggaa ccagaaatct
     5521 agtgtcctgt cccggagatt cgtgatgtaa gtcgttcaag cttctgaagg atggaatcag
     5581 ccgtggcggt ccaggtaaag ggttggcatt ttttgttgta ctgggtgaca aagtgatcaa
     5641 ttttgccgat caactcctt
//
LOCUS       DATNDD010000128         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3275615, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000128 DATNDD010000000
VERSION     DATNDD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275615"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VN647_03100"
     CDS             complement(<1..421)
                     /locus_tag="VN647_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96050.1"
                     /translation="MILPPNEWFAIDSSVALDGLKSLILLLTLVIVRSLIVRWIAHNP
                     TLSMESKRRWVVTTRNSVVFAFLIGLVIIWAHELQAFAVSLVALAAAFVLATKELLLC
                     WSGAALRVGGKVYAVGDRIQIAGHRGVVLDHDVFATKL"
     gene            complement(425..871)
                     /locus_tag="VN647_03105"
     CDS             complement(425..871)
                     /locus_tag="VN647_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96051.1"
                     /translation="MSVSISSAVQKYLPLVTGALLAALAIGFSGCGGPWRDSYFKKGV
                     GRLTQEEIRERLGPPHTAKTPALGGDSLWTYRFALSEQDLTSWNSSFVADASHSVTSM
                     MGKGTELAKPTLYCYRYILMFSEDKTLKNWKREECVPGTRETLTAK"
     gene            1022..1999
                     /locus_tag="VN647_03110"
     CDS             1022..1999
                     /locus_tag="VN647_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWG96052.1"
                     /translation="MDVRLIVTACIAGVATLSLQTGFAATESRTAKPESTSPTSLRSD
                     LPPVSAKPPGSTVDSKTGARSKSPTNGGAAYQLTVLIAASSEIRGKDGAPMVLVPAGE
                     FVMGSDKGDEDEAPVHRVYLNAFYIDKFEVTNGRFAKYVEAIQSEPPWGFTDKEMPVI
                     HAERPVRWVNWMDAMGYCLWIGKRLPTEAEWEKAARGTDERVYPWGNDPPTPVHAVYG
                     LKEGGAETVSVIGNHHMGQSPYGVQDLAGNLYEWVMDWYSEDFYSSFINSPAINPRGP
                     NEGTAKVQRGGSYINTPYRLRSSFRTKDDPTEHDPNVGFRCAQDIQKQP"
BASE COUNT          494 a          588 c          623 g          428 t
ORIGIN      
        1 ataacttcgt cgcaaagacg tcgtgatcca gcacgactcc gcggtggccc gcgatttgaa
       61 ttcgatcacc gacggcatag actttgccgc ccactcgaag tgccgcgccg ctccagcaga
      121 gaagcagctc tttcgtcgcc aagacgaacg ccgccgccaa ggcgacgagc gaaacggcaa
      181 aggcttgcag ttcgtgggcc cagatgatta cgagaccgat gagaaacgcg aagacgaccg
      241 agttcctggt cgtgaccacc cagcggcgtt tcgattccat cgagagcgtc ggattgtggg
      301 cgatccaacg cacgatcagc gacctgacaa taaccaaggt cagcaacagg atcagcgact
      361 tcaatccgtc caacgcgacc gagctgtcga ttgcaaacca ctcatttggg ggcaatatca
      421 tcctctactt ggcggtcaat gtttcgcggg tacccggcac gcattcctca cgtttccagt
      481 tcttcagggt cttgtcttcg gagaacatca agatgtaccg atagcaatac aacgtgggtt
      541 tggcgagttc ggttcccttg cccatcatgc tggtgaccga gtgagaagcg tcggcgacga
      601 acgaggaatt ccacgaggtg aggtcctgtt cgctgagtgc gaaccgatag gtccagagac
      661 tgtccccgcc gagagccggg gtcttggccg tgtgcggggg acccagtcgc tctcgaatct
      721 cctcctgtgt cagccgacca actccttttt tgaaataact atctctccag ggaccgccac
      781 agccggaaaa gccgatggcc agagcggcaa gcagggcacc ggtaacaagg gggaggtact
      841 tttggacggc ggaagaaatc gagaccgaca tgctgacgct tacgctacac cggacgaccg
      901 gtaagtgcaa ctccatagac catcaccgct atcatgttcg gctggcggta aagtggcaag
      961 ccgaggcggg ctggatttgg ccatacgttt tcggtacctt agtattgacc gaggccaacg
     1021 tatggatgtc cgactgattg ttaccgcgtg cattgccggt gtcgcgaccc tctctctaca
     1081 gacggggttt gcagcgaccg aaagccggac cgccaagccc gagtccactt caccaacttc
     1141 cctgaggtct gatcttcctc ccgtatcggc gaaaccacca ggctccactg tcgattccaa
     1201 gactggagcg aggagcaagt cgccgaccaa cggtggcgct gcatatcagc tgactgtgct
     1261 gatcgctgcc tcttccgaaa tcagggggaa agatggtgcg ccaatggtgc tggtgccggc
     1321 cggtgagttt gtgatgggaa gcgataaggg tgatgaagac gaagcccctg tgcaccgtgt
     1381 gtatctcaat gccttctaca tcgacaaatt cgaagtgacg aacggacgat tcgccaagta
     1441 tgtcgaggcc atccagagtg aacctccctg gggatttacg gacaaggaga tgcccgtgat
     1501 ccatgccgag cgtccggtcc gctgggtgaa ctggatggat gcgatggggt actgcctctg
     1561 gattggcaag cgacttccca cagaagccga atgggagaag gctgcgcgtg gaactgacga
     1621 aagggtctac ccatggggca atgatccacc gactccggtg catgcggtct atgggttaaa
     1681 ggaaggcggc gcagagacag tgtcggtcat cggcaatcac cacatgggac aaagtccata
     1741 cggtgtacag gatctggcgg gtaatctcta cgaatgggta atggactggt actccgagga
     1801 cttttattcc agctttatca acagccccgc tatcaatccg cgcggaccca acgagggaac
     1861 ggcaaaagtc cagcgagggg gatcctacat caataccccg tatcgtctgc ggtcgtcgtt
     1921 tcgcacgaag gatgatccga ccgagcatga tccgaatgtg ggcttccgct gtgctcagga
     1981 tattcagaag cagccgtaat ctcgtcagtg ccgcgattca cgcgtcctgg actcgaagac
     2041 cttacggctg ttggcatctg ctatgcacta cgcatcggtg ccaagaaaag ctgagtgaca
     2101 cttgacaagg ttttgcaaag agcgtaggaa gaa
//
LOCUS       DATNDD010000129         3573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3809443, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000129 DATNDD010000000
VERSION     DATNDD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809443"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..859)
                     /locus_tag="VN647_03115"
     CDS             complement(5..859)
                     /locus_tag="VN647_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96053.1"
                     /translation="MTRPSFVPLGLIFSLIFLLCFLTPATSQALADSSLNEPQPTTVL
                     VRVVAHGAMVLGREVGGARVTITDVASGHILATGLQQGESGDQNQIMRTPHLMEEPIY
                     SARPSAAFTTTLELQKPTVVEISAEGPLAYPAALQRASKTLLLIPGQDLTNDGIVLHL
                     YGYLVQIERPKPREALIAKDDVKLRASVRTLSGSLVRPYGDWDSRKIRIYGEVLIGDR
                     IIERLQLFYDEGSRAFEAPFFVPPSKEVPDGIKLRVIATDLSTGNSGMDQANYPVLSE
                     RLPPKSVR"
     gene            complement(856..2826)
                     /gene="metG"
                     /locus_tag="VN647_03120"
     CDS             complement(856..2826)
                     /gene="metG"
                     /locus_tag="VN647_03120"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWG96054.1"
                     /translation="MSRDDTFYVTTPIYYVNDVPHIGHAYTTVAADVLARYWRLRGHE
                     VFFLTGLDEHGQKVQQAAAKAGIAPQAHCDKLAPKFETLWKKLNVSNNAFIRTTDPEH
                     KFIVQRYLQQLYDKKLIYKDSYTGWYCTFDERFWTEKDVESGLCPDCKRPVEKLSEHN
                     YFFKMGQYQDRLIEHIKSNQDFIRPESRRNEVLGFLQNQKLGDLSISRPKSRLSWGIE
                     LPFDNNYVTYVWFDALVNYISALEYLPRENPAGNRFWPANIHLVGKDILTTHAVYWST
                     MLMALNLPLPETIFAHGWWTVDGEKMSKSRGNVVDPNKMADEFGTDAFRYFLLREVPF
                     GQDGDFSLTGMVARINSDLANGLGNLLSRTLTLIERTQAGLLPAPGLMKPAERELEQA
                     TLSLLNEVMPRHFDQLEFNRVLEAIWHVVQLANQYVDKTAPWNLAKNQSDAEQLRTVL
                     YTMAETLRCISLAAYPVIPKSAQSICSQLGISAEFTSPLLRSKIEWGGLKPGTLIHKG
                     PSLFPRIESKPGARPVTESSVPSQPTPTAPTATAVPAPPAPPQITIDEFMKIQLKTAK
                     VLSAERVPKSEKLIKLQVSLGTEQRQIVAGIGKKYDPDALIGKTIVIVANLKPAKLMG
                     IESQGMVLAAGDTDVHGLLTIQEEVDPGTKVK"
     gene            complement(2863..>3573)
                     /locus_tag="VN647_03125"
     CDS             complement(2863..>3573)
                     /locus_tag="VN647_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HWG96055.1"
                     /translation="FVYRPLVGERKICLIDDADRLTIGAANALLKTLEEPPGQSLFIL
                     ITSRLYALPITIRSRCQALRFTTPARTQVEAALILKRELPPIDARFLAVFSDGRIGEA
                     LTVNVAEVRARQQECLTLVKPESLTSSTTILSASESLAKTDRGEETLAWLTRWIRDLV
                     FVIVDGDQDQLLHLDQLTDLRRYAQRADIDTLLTLLNDIERTEQQATRHLNVQMALET
                     TFLRLREALGLAPAGASA"
BASE COUNT          745 a          945 c         1046 g          837 t
ORIGIN      
        1 ccggctaccg aacggatttg ggcgggagac gctcgcttag cacaggatag ttggcttgat
       61 ccattcctga attgcctgta gacaggtccg tcgcaatcac tcgaagcttg atgccatcgg
      121 gaacctcctt cgacggcggc acaaagaacg gcgcttcaaa ggcgcggctc ccttcgtcat
      181 aaaacagttg tagtcgctcg atgattcggt ctccgatcaa cacctctcca taaatacgga
      241 tcttccgcga gtcccagtct ccataggggc ggaccaatga accggacagg gttcggactg
      301 aggctcggag tttcacatcg tcttttgcga tcaatgcctc acgtggcttg ggccgctcga
      361 tctgaaccag gtagccatag agatgcagga caatgccgtc gttcgtcagg tcctgtcccg
      421 ggatcaacag caaggttttg ctggcccttt gcaatgcagc cggataggcg agcggccctt
      481 ctgcggagat ctccactacc gtcggtttct gcagttccaa cgtggtggtg aaggcggcag
      541 aaggacgagc gctgtagatc ggctcttcca tcagatgggg ggtacgcatg atttgattct
      601 gatctcctga ttcgccctgt tgcaggccgg ttgccagaat gtggccgctc gcgacatcag
      661 taatggtcac gcgcgctcca ccgacttcac ggcccaacac catcgctcca tgcgcgacga
      721 cccgcacgag cactgtggtc ggctgaggct cgttgagcga tgaatcagcg agggcttggc
      781 ttgtggcggg agtcagaaag caaaggagga agatgagcga gaaaatcagt ccaagtggga
      841 caaacgaagg tcgtgtcatt tcaccttggt gccgggatcc acttcttcct ggatggtgag
      901 cagcccgtgt acatcggtat cgcctgccgc gagtaccata ccctgtgatt caatgcccat
      961 gagcttggcc ggcttcaagt tggccacgat cacgatggtt ttgccgatca gcgcgtccgg
     1021 atcgtacttc ttgccgatgc ctgccacgat ttgacgttgt tcggtgccga gggacacttg
     1081 aagcttgatc agcttctccg atttcggcac acgttctgcg gagagtacct tcgccgtctt
     1141 gagctgtatc ttcatgaatt cgtcgatcgt gatttgcggc ggagccggag gtgctggaac
     1201 ggctgtggcg gtgggtgctg ttggggttgg ttgtgaagga acagatgatt cggtcactgg
     1261 tcttgctcct ggttttgatt cgatgcgggg gaacagcgac ggtcctttat ggatgagggt
     1321 gccgggcttc aggccacccc attcgatctt gcttctcagt aaagggcttg tgaattcagc
     1381 tgagatgcca agttgagaac agatcgactg cgcactcttc gggatcacag gatacgcggc
     1441 caagctgatg cagcggagcg tctcagccat ggtatagaga acggtcctca gttgttctgc
     1501 gtcactttgg ttctttgcca gattccacgg ggcagtctta tcgacatact gattggcaag
     1561 ctgaacgacg tgccatatgg cttcgagcac ccgattaaat tccaactgat cgaagtggcg
     1621 cggcatcact tcgttcaaga gagacagcgt ggcttgttcc aactcccgct ctgccggttt
     1681 catcagcccc ggagccggca gcagccctgc ctgcgtccgt tcgatgaggg ttagtgtacg
     1741 actcaaaaga ttgcccagac cattggcgag atcgctattg attcgtgcaa ccattccggt
     1801 aagcgagaag tctccatcct gcccaaaagg cacctctctc aataagaaat agcgaaaggc
     1861 gtccgtgccg aattcgtcgg ccatcttgtt cggatcgacg acgttgccgc gactcttcga
     1921 catcttctcg ccatccacgg tccaccagcc atgggcgaag atggtctctg gtaacggaag
     1981 attcaatgcc atcagcatcg tggaccaata gacggcatgt gtcgtcaaga tatccttccc
     2041 gacaagatgg atattggccg gccagaatcg gttgccggct gggttttccc tggggagata
     2101 ttccaaggcg gagatgtaat tcaccaaggc atcaaaccac acataggtca cataattgtt
     2161 atcaaatggg agttcgattc cccaagagag ccgcgatttc ggcctcgaga tcgagaggtc
     2221 gccgagtttc tggttttgta agaagcccag gacttcgttg cgtcgtgact ccgggcgaat
     2281 gaaatcctga ttggatttga tgtgctcaat cagacggtcc tgatactgcc ccatcttgaa
     2341 gaagtagttg tgctcgctga gtttctcgac ggggcgctta cagtcaggac agagtccgga
     2401 ctccacgtcc ttttcggtcc agaaccgttc atcgaaggtg cagtaccatc cggtgtatga
     2461 atccttgtaa atcagctttt tgtcatagag ctgctgcagg tatcgttgga cgatgaattt
     2521 gtgctcggga tccgtcgtcc tgatgaaggc gttattcgag acgttcagct tcttccagag
     2581 agtctcaaat ttcggcgcga gcttgtcaca gtgggcttgt ggggcgatac cggccttggc
     2641 ggctgcctgt tgcactttct ggccatgttc atcgaggccg gtgagaaaaa acacctcgtg
     2701 cccacgcagc cgccaatagc gcgccagcac gtccgccgcc accgtggtgt aggcatggcc
     2761 gatatgcggg acatcgttga cataataaat cggcgtggtg acgtaaaagg tgtcatctct
     2821 agacataacg aaggcaagat atcaggttgg catcgtttat tactaggcgg aagctccggc
     2881 gggggccagc ccgagtgcct cgcgcaggcg aaggaacgtc gtttccagcg ccatctgcac
     2941 gttcaagtgc cgcgtggcct gttgttcggt ccgctcgatg tcattcaaga gggtcaggag
     3001 cgtgtcgata tccgctcgct gggcataacg tcgtaggtcg gtgagttggt cgaggtgaag
     3061 gagctgatcc tgatctccat ccacgatgac aaagacaaga tcacggatcc atcgcgtaag
     3121 ccaggcgagc gtttcttctc cacggtctgt cttggccaag ctttccgatg cagagaggat
     3181 ggtcgtgctc gacgtcaagc tttccggttt caccaaagtc aggcattctt gctgtcgcgc
     3241 tcgaacttct gcaacgttca ccgtgagcgc ttctccgata cggccatcgg aaaagaccgc
     3301 caggaagcgg gcatcgatag gaggaagctc tcgcttgagg atcagcgccg cctcaacttg
     3361 cgtgcgggcg ggcgtcgtga aacggagcgc ttggcagcgt gatcgaatgg tgatcggaag
     3421 cgcatagagt ctgctggtga tgaggatgaa gagactctga cccggaggct cttccaaagt
     3481 tttgaggagc gcgttggctg ccccgatcgt cagtcggtcc gcgtcgtcaa tcaggcaaat
     3541 cttccgttcc ccgacgagtg gtcgatagac gaa
//
LOCUS       DATNDD010000130         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3514874, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000130 DATNDD010000000
VERSION     DATNDD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3514874"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /gene="tuf"
                     /locus_tag="VN647_03130"
     CDS             <1..140
                     /gene="tuf"
                     /locus_tag="VN647_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWG96056.1"
                     /translation="MVMPGDNVSVTAELISPIAMDQGLRFAVREGGKTVGSGVVTEIL
                     A"
     gene            243..392
                     /gene="rpmG"
                     /locus_tag="VN647_03135"
     CDS             243..392
                     /gene="rpmG"
                     /locus_tag="VN647_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWG96057.1"
                     /translation="MREIIDMACTLCKQRNYSSMKNKKNDPDRLERNKFCKFCRKHTP
                     HKEVK"
     gene            467..541
                     /locus_tag="VN647_03140"
     tRNA            467..541
                     /locus_tag="VN647_03140"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Trp,seq:cca)
     gene            714..908
                     /gene="secE"
                     /locus_tag="VN647_03145"
     CDS             714..908
                     /gene="secE"
                     /locus_tag="VN647_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWG96058.1"
                     /translation="MFKRMTDSVRLFVTDVRAELKKVSFPSRAETIGSTTVVIVFCIL
                     MSLYLSVIDSFLSWLVGKFI"
     gene            970..1506
                     /gene="nusG"
                     /locus_tag="VN647_03150"
     CDS             970..1506
                     /gene="nusG"
                     /locus_tag="VN647_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWG96059.1"
                     /translation="MTKNWYVIHTYAGFEGRVKTSLMERANQMGLVEKVGQVLVPTED
                     VIEIKDGKRRTSRRKFFPGYVLVELESPLGDETLQMIKETPKVTGFVGGGAVPTPLTD
                     EEVESLLKQVDAGQAEPREQVKFIKSDNVRIIDGPFLGFNGMVDEVDQDHSRLKVMVS
                     IFGRSTPVELGFLQVERI"
     gene            1587..2012
                     /gene="rplK"
                     /locus_tag="VN647_03155"
     CDS             1587..2012
                     /gene="rplK"
                     /locus_tag="VN647_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWG96060.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKEGDSIIPVIITVYKDRTFSFIMKTPPASDLLKKAAGIIKGSGVPQKDKVGKITR
                     QQLNEIARKKMTDLNAADVEGAAKIIEGTARSMGVVIQG"
BASE COUNT          546 a          459 c          650 g          462 t
ORIGIN      
        1 agatggtgat gccgggcgac aacgtgagtg tgacggcgga gttgatcagt ccgatcgcga
       61 tggatcaggg gttgcggttt gccgtgcgcg agggcggcaa gacggtcggc tcgggcgtcg
      121 tcaccgaaat tctggcgtaa ggaaaaggat gttaatcgtc acgcgtcaac cgtcattgga
      181 gagaagagac gcttccagat ttgcgattga cgatcggcag atgacgaatt acggagcaga
      241 aaatgcgtga aatcatcgac atggcttgta cactctgcaa acagcggaat tactcgtcca
      301 tgaagaacaa gaaaaacgat ccggatcggt tggaacgaaa caaattctgt aaattctgcc
      361 gaaagcacac gcctcataag gaagtgaagt aattgctgag acataagtgt tgagtattgg
      421 gatcatcggc aggcgaaaaa ctcgaccctc agcactgaca aagagtaggg gcatggtgtt
      481 aatggcagca catcggtctc caaaaccgag agtctgggtt caaatcctag tgcccctgcc
      541 attccgagcg cgctcgtacc gactgccatt agagagctca ttggcggccg gaggaagcac
      601 cctggggttg tagttggggg tatggcgaca gcgaatgcct gttctagttg tgggttcagg
      661 tttgatagtc gcttggaatt tgcaggctcg gacactagaa aacggagtga ttgatgttta
      721 agcgcatgac ggactcggtt cggctgttcg tgacggacgt gcgggcagag ctgaagaaag
      781 tttcatttcc gagtcgtgcg gaaaccatcg gctcaacgac ggtggtgatc gtgttctgca
      841 ttctgatgtc gctgtactta tccgtcattg actcattcct ttcatggttg gtcggcaaat
      901 tcatctagac ctgccgacaa gagctaaggg gaattcagcg gtaatttatc gaagacctga
      961 gggtggtgca tgacaaagaa ctggtacgtc atccatacgt acgcgggttt tgaggggcga
     1021 gtgaaaacca gcctcatgga gcgtgcaaat caaatggggc ttgtcgagaa ggtcggccag
     1081 gtgctcgttc cgaccgagga cgtgattgaa attaaagacg ggaagcgacg gacttctcgg
     1141 cgaaagttct ttccaggata cgtcctcgtg gagttggagt ctccgttggg ggatgagacc
     1201 ttgcagatga tcaaggagac accgaaagtt acaggatttg tcggaggagg tgcagttccg
     1261 actccgctga ccgatgagga agtggagtcc ttgctcaagc aagtcgatgc aggtcaggcc
     1321 gagcctcgag agcaggtcaa atttattaaa agcgacaacg ttcgcatcat cgacggcccc
     1381 ttccttgggt tcaacggtat ggtggacgaa gtcgatcagg accatagtcg gttgaaggtg
     1441 atggtgagca tcttcggccg gtcgaccccg gttgaattgg gattcctgca ggttgaacgg
     1501 atttaagccg atcgatcgac gtcagccttg agtcatgatt gaaggctgaa agttgattgt
     1561 cgatctcgaa ggagaaagaa aacgaaatgg cgaaagaagt ttcggcgcaa atcaaattac
     1621 aaattccggc cggcaaggcc aatccggctc ctcctgtcgg cccctccttg ggtcagcatg
     1681 gggtcaatat tatggagttt tgcaagcagt tcaatgccaa gacccaaaag gaaggggaca
     1741 gcattatccc cgtcatcatt acggtctata aggaccgcac gtttagcttc atcatgaaga
     1801 cgcctccggc ttcggatctc ctgaagaagg ctgcggggat catcaaagga tctggtgtgc
     1861 cgcagaagga taaagttggg aaaatcacca ggcagcagtt gaacgaaatt gcgcgaaaga
     1921 aaatgaccga tctgaacgcg gctgatgtgg aaggggcggc aaaaatcatc gaagggaccg
     1981 cccgtagcat gggcgtcgtc attcaaggct aatctcggga tcgaaggagc gtctggttat
     2041 gggaaagaaa atggatgcgg cgatcaagaa tgtcgagccg cgcgtctatg ggttgcgtga
     2101 ggcggtcgaa accgtga
//
LOCUS       DATNDD010000131         2457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2622324, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000131 DATNDD010000000
VERSION     DATNDD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622324"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VN647_03160"
     CDS             <1..619
                     /locus_tag="VN647_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWG96061.1"
                     /translation="NHMQTVREQLLLMRVVDLHIRGNITVGDSELKRFYQEHRDRFAL
                     PEEYQLSQIIIQPRSSDGLAEALTKARRAMDDLKRGEKFEDVAMQYSDGANALQGGRL
                     GWVRQGELIRVIEQAVAPLVPGGISNIIESPEGIQIIRMDDRKPKQFRRYEDARREIQ
                     EMVYRQKSDDMYESWLIDLKNKAYIEIKFEQVADQTQPQPVRSTP"
     gene            complement(627..1247)
                     /gene="yihA"
                     /locus_tag="VN647_03165"
     CDS             complement(627..1247)
                     /gene="yihA"
                     /locus_tag="VN647_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWG96062.1"
                     /translation="MKIFAAEFIKSCVTPEQFPSGQLHEIAFVGRSNVGKSSLINSLL
                     NRRDLAKVSRTPGKTRAVNVFLVSTSDPDLSQFHLVDLPGYGFAKVSKSVRTQWGPLM
                     EDYLVGRASLLGIVMLVDSRVVTDQDRQTIAWLRSIRRNPLIVATKVDKLKPNERVRT
                     LRQTHHALGLADGELLIPYSSETGDGRDRVWGALREVAGRRSTDIS"
     gene            1273..2073
                     /gene="mtgA"
                     /locus_tag="VN647_03170"
     CDS             1273..2073
                     /gene="mtgA"
                     /locus_tag="VN647_03170"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HWG96063.1"
                     /translation="MDARRKASHSPTFLRHYTYDPPSPKSTGRRRVAPVLPWSTLLIG
                     LPLSILALSWLSTLPDTDLLARTNPTSTALMDHRLTRAAEQGRTGKRQWIWTPLSRIA
                     PELQRAVITAEDASFFVHEGFDWEGIRDAALYNLEAGELKRGGSTITQQLAKNLYLSS
                     ERSLFRKAREALITRSLEHQLTKRRILELYLNVAEWGRGVYGAEAAARHHFGKSASDL
                     TADEAAWLAAILPSPQRYDPIRKTTALTRRHQRILRWMDKVSIQRVNS"
BASE COUNT          576 a          719 c          668 g          494 t
ORIGIN      
        1 gaaccatatg cagaccgttc gcgagcagct cctcctcatg agagtcgtgg acctgcacat
       61 tcgcggcaac attaccgtcg gagactctga gctcaaacgg ttctaccaag aacatcgcga
      121 ccggttcgct cttcccgaag aataccaact gagccagatc atcattcaac cgcgctcctc
      181 ggatggattg gccgaggcct tgaccaaagc ccgccgagcc atggacgact tgaagcgagg
      241 agagaaattc gaagatgtcg ccatgcagta ttccgacggc gccaatgcct tgcaaggagg
      301 acgactggga tgggtccgac agggggaact cattcgcgtc atcgaacaag cggtcgctcc
      361 cttagtgccg ggaggaatct cgaacatcat cgaaagcccc gagggaatcc agattatccg
      421 catggatgac agaaagccga agcagtttcg ccgatacgag gacgcacggc gggaaattca
      481 ggaaatggtc tatcgacaga agagcgatga catgtatgaa tcctggctca ttgatctcaa
      541 gaacaaagcg tacatcgaaa tcaagttcga acaagttgcc gaccagacgc agccacagcc
      601 tgtgcgttcg acgccatagg tagcgatcaa gaaatgtctg tggaacgtct tccggctacc
      661 tcgcgcagag ctccccacac ccgatcccgt ccatccccgg tctccgacga atagggaatc
      721 aacagttcgc cgtcggctag tcccagagca tggtgcgtct gcctcaaggt gcgcacccgt
      781 tcgttgggtt tcaacttatc cacctttgtc gccacgataa ggggattccg gcggatcgaa
      841 cggagccacg caatggtctg acgatcttgg tcggtcacga ctcggctgtc caccaacatg
      901 acgatcccga ggagcgaggc tcgaccgacg agatagtctt ccatcaaagg cccccactgg
      961 gtgcggacgg atttcgacac tttggcgaat ccgtagccgg gtaggtccac gaggtgaaat
     1021 tgcgagaggt ccggatcgga agtcgagacg aggaacacat tcacggctct tgtctttcca
     1081 ggagtccggc tgacttttgc caggtctcga cgattcagca acgaattgat caacgacgat
     1141 ttacccacat tggagcgtcc cacaaaagca atttcgtgaa gttgaccgga cggaaattgt
     1201 tctggagtga cgcaactttt gataaactca gctgcgaaaa ttttcatgcc gtcaggaccg
     1261 tcaggctgat cgatggatgc aagacggaag gcaagtcaca gtccgacctt tctgcgtcac
     1321 tacacatacg atcctccttc gcccaagtca accggccgac gcagagtggc ccctgttctt
     1381 ccctggagca ccctgctcat cggacttcct ttaagcatcc ttgcgctgag ctggctcagc
     1441 actcttcccg acacagacct cctggcaaga accaacccga catcgacggc gctcatggac
     1501 catcggttga cgagagcggc cgaacaggga cgcaccggca agcgacaatg gatctggacc
     1561 cccttgagcc gaatcgcgcc ggagttgcaa cgtgccgtga tcacggcgga ggacgcctca
     1621 ttctttgtcc atgagggatt cgattgggag ggcatccgcg acgccgcgct gtataatctt
     1681 gaagccggag aactcaaacg aggcgggagc accattaccc agcagctggc gaagaacctg
     1741 tatctgtcat cagaacgttc gctgttccgt aaagcccgtg aggccttgat cacgcgctcg
     1801 cttgaacatc agttgaccaa gagacgtatc ctcgaactgt acctcaatgt cgcggaatgg
     1861 ggacgcggcg tgtatggggc ggaagccgcg gctcgtcatc attttggaaa atccgcctcg
     1921 gacctgactg cggacgaagc cgcttggctg gccgccattc tccccagtcc gcagcgatac
     1981 gaccccatca gaaaaaccac tgcactgacg cgccgacatc agcgcatcct tcggtggatg
     2041 gataaggtat cgatccagcg ggtcaattcg taaccactgc atagcggcaa tgcttcgatt
     2101 cggcttagga tgccgccgaa tgtgcgcctc caatcaagag cgtgcgaagc agttccaatt
     2161 aacctcaatc cgaaaataat tctggtcctg ctggcttatt ctgcgggtcc tgtcgtggcg
     2221 aacaacccca ccgtgctcac tccacccatc ccccagtagg ggcccttccg ttgcgcgcgg
     2281 tggggcgccc gttcgccacg ccacccacgg tcatttcgct tccgtgcctt gagattattt
     2341 ttggattggg gctaaaagag ggggagttca aggctttgag attccaaact ggaatgtcaa
     2401 agctgtcgag acggcggcac ctgcactagg catgcctacg ttcatcaacg atcgctt
//
LOCUS       DATNDD010000132         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1435820, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000132 DATNDD010000000
VERSION     DATNDD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435820"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..815)
                     /locus_tag="VN647_03175"
     CDS             complement(51..815)
                     /locus_tag="VN647_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96064.1"
                     /translation="MNGDQPSRDVIALKRLEDEIERRRIHVYRNLLGQPYVFFDIVEG
                     TLADTQPAIHLHLYHKDVRGWLTQFIWKVEKILLHERELDRVLQALAGLSLRNKLDRV
                     TDPALLSLLETEPTVAVIVEFMHMEKQSRFEFTMESLWKRLRDFARERGLLLRGRKHF
                     PGGANVLSRKLAIYEKELLRLGIKLTMTRSNGCKVVLERLDDSTSQPSAESSTPNSSA
                     LNELSPEDAKQSMLATLANKRRQQVDQPPHPTTEGT"
     gene            complement(1045..1602)
                     /locus_tag="VN647_03180"
     CDS             complement(1045..1602)
                     /locus_tag="VN647_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96065.1"
                     /translation="MSLFHMKIQAILDVISVYEEEGQHERLDADHMLYLLVEDFRKSL
                     PGQRIPTAGRTLWYSVLGNSRDCPEDVATPLSQFADWLIQNYLTERELVIHIRSGSLE
                     FYGNSYSNIDPNTLALLEFLQQHPGEKFSSSDLARKIPGLRGNPSMIRNLLKELEKIE
                     PRLRACVKGKTGAGRWLEVPRRHYT"
     gene            2069..>2439
                     /locus_tag="VN647_03185"
     CDS             2069..>2439
                     /locus_tag="VN647_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96066.1"
                     /translation="MARTPSPWFWEERNGWYVTKDGQRHFLGEHFPDSPKPKKMRGKW
                     NAPDSIREQFHSLMAAKPVPASNDSLSLAGLFEKYLEWCQRHRAARTYEGYQWHLQAF
                     CDHLGNTARTAAGGLKPFHVI"
BASE COUNT          522 a          638 c          713 g          566 t
ORIGIN      
        1 gtccgggcgt atggtgcgaa caggtgggat actgacagta ttcgcgttca tcaggtacct
       61 tcagttgttg ggtgtggtgg ttgatcaact tgctggcgac gcttgttggc cagggttgca
      121 agcatggact gttttgcgtc ctccggcgac aactcgttca gggctgagga attcggtgtg
      181 gacgattccg cggacggttg agaggtggaa tcgtccaacc gttccaggac gaccttgcag
      241 ccgttggatc gggtcatggt cagcttgatg ccgagacgta atagttcctt ctcatagatg
      301 gccagcttcc tgctcaggac attggctcct ccagggaaat gctttctgcc tcgtagaagg
      361 agacctcgct cccgggcgaa gtcgcgaagt ctcttccaca gcgactccat tgtgaactcg
      421 aaccgggact gtttctccat gtgcatgaac tcgacgatga ccgcaaccgt tggttcagtc
      481 tccaggagag aaaggagggc aggatcggtc acgcgatcca gtttgttgcg aaggctcagt
      541 ccggccagtg cctgtagcac gcgatcgagt tctcgttcgt gaagcaggat cttttccacc
      601 ttccagatga actgggtcag ccatccgcga acgtccttgt ggtaaaggtg cagatggatc
      661 gcgggctgcg tgtcggccaa ggtaccctcg acgatatcga agaaaacgta cggttggccg
      721 agcaggttgc ggtagacgtg aattcgccgc ctctcgatct cgtcttccaa acgtttcaga
      781 gcgatcacgt ctcggctcgg ctggtctcca ttcatgagat ggcatcatct cccacatccc
      841 atcctgtaat cgcggcgacc agagcgacgc ttttttgccg cctgcctatt cacaggtgaa
      901 ggacaagtgc cccggtcctg agtgattgaa ctcgcaacgt gagcggccgc ttccgcgtac
      961 tgggtcgcgg atttgatcgt aaggtaatcg ctggttccaa acaaaagctc agatatgtct
     1021 agttgcggct agttgaggcg aacgtcaggt gtagtggcgt cttggcacct ccagccatcg
     1081 acctgcaccg gtttttccct tcacacatgc acgaagacgc ggctcaatct tctccagctc
     1141 cttcaggaga ttgcggatca tcgaagggtt gcccctgagg ccagggattt tccgggccag
     1201 gtcagaggat gagaactttt ctccaggatg ttgctgaagg aattcaagca aggcgagggt
     1261 attcggatcg atgttcgagt aagaattgcc gtagaactcg agagagccgc tccgaatgtg
     1321 aataaccagt tctctttctg tcaagtagtt ctggatcaac cagtctgcga actgactgag
     1381 aggagtggcg acatcttcgg ggcagtctcg cgagtttccg agcacgctgt accaaagcgt
     1441 ccgaccggca gtgggaatgc gttgccccgg cagcgatttc cgaaagtcct cgactagtag
     1501 atacagcatg tgatcggcat ccaggcgttc atgctgtcct tcctcctcgt atacactgat
     1561 gacatcgaga atggcctgga tcttcatgtg gaacaaggac atcgcagatt tccgctcagg
     1621 agaagttgac ctgaagccga attctactgc ttacctgggg catgtgaagc gatgtactcc
     1681 gagccgccag tcgtgcgttg gaggagagcg gaggtagtaa ctgtggtggc gatgagaagc
     1741 tttcaccagg gtattgtcag gtgactggag ctagtgaatg ttcagatacg acggctcctc
     1801 tcaggagggt acacttttag aaactgtacc ctacaaaggc catattcgtg gtttcgtgag
     1861 atgattgcgg tttttcgaaa ccccatgaaa caaggcatcc ggtgggtatc cgaaactctc
     1921 tcggaacacg tcttggcact acggaaccga aggttgcagg ttcgactcct gctgggtgta
     1981 tttggattta cggcgagtcg ctggtaagat ggtacagaat gacggtacag aataatcccg
     2041 gggccgcgac tcccccgagg aattccagat ggcccgcacc ccgagcccgt ggttctggga
     2101 agaacgcaac ggctggtatg taaccaagga cggacagcga cattttctgg gcgagcattt
     2161 tccggactcc ccgaagccga agaagatgcg cggcaagtgg aatgccccgg actccatccg
     2221 agagcaattc cactcgctca tggccgcgaa gcctgttccg gcctcgaacg attcactttc
     2281 ccttgccggc ctctttgaaa agtacctcga atggtgccag aggcatcgtg ccgcccggac
     2341 gtacgaaggt tatcagtggc atctccaagc gttttgtgac catctcggca atacggcgcg
     2401 cacggccgcg ggtggactga agccgttcca tgtcatcga
//
LOCUS       DATNDD010000133         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1552106, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000133 DATNDD010000000
VERSION     DATNDD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552106"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VN647_03190"
     CDS             <1..253
                     /locus_tag="VN647_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96067.1"
                     /translation="LGRGRLNYCRRLVERVRDLSSLRWAMMGVGMHSAALARDLALQL
                     GRTVGSGSRRSWGSASQAEKDQWQSVWAGLARDHTVIHA"
     gene            278..2215
                     /gene="asnB"
                     /locus_tag="VN647_03195"
     CDS             278..2215
                     /gene="asnB"
                     /locus_tag="VN647_03195"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWG96068.1"
                     /translation="MCGIAGMMFCLDAELSPIVSKMVEMIRYRGPDDSGLWCDADYGL
                     ALGHARLSILDLSPAGHQPMESASGRYVIVFNGEIYNHLELRGQLTGLPWRGHSDTET
                     LLTAFETWGVEKALQATVGMFALALWDRIERRLVLARDRIGEKPLYYGWSNGTFIFAS
                     ELKALEAYPGWRGEIDRDALALFMRYAYVPLPYSIYTGIRKLLPGTYVTIPSECPARY
                     WSEPKPYWSAAAVAGQPIESEWTDMAAEDELHRLLSRAIKGQMLSDVSLGALLSGGID
                     SSTVVALMQAQSSRPVKTFSIGFHDDEYNEAPQAKAVATHLGTEHTELYVRSADALAV
                     IPHLPTMYDEPFGDSSAIPTHLVARLAKQQITVALSGDGGDELFGGYNRYSWGRSIWH
                     RIARVPIPMRAMTAAALTMLSPEQWDRIGRILRPGLPASLHMSALGEKVHKLAAVLDS
                     DSQTELYRRLVSLQRETSSLVIGAQDKPIWADAQAQQLGGRNFSEEMMLHDLVGYLTD
                     DILAKVDRAAMAISLETRMPLLDHRVLEFSWSLPLSMKIREENQGKWLLRQVLYRYVP
                     KDLVERPKMGFGIPLDSWLRGSLRDWAEALLEESRLKREGYFDPAPIRAKWREHLSGR
                     RNRQYWLWNVLMFQAWLSAHE"
     gene            2208..>2576
                     /locus_tag="VN647_03200"
     CDS             2208..>2576
                     /locus_tag="VN647_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG96069.1"
                     /translation="MSDRPRLLYLITEDWYFWSHRVDLARAAREAGYDVIVATRVTDH
                     GARIRGEGFQLEPLELVRRSRNPLREAVAVAELVRLYRRVRPDIVHHVAMKPILYGSL
                     VAWFTRVPAVINAFAGLGYTF"
BASE COUNT          493 a          675 c          801 g          607 t
ORIGIN      
        1 attgggccgt ggtcggctca actattgtcg acgcctggtc gagcgggtgc gcgacctttc
       61 ctcattgagg tgggcgatga tgggcgttgg tatgcatagc gccgctcttg caagagacct
      121 ggcccttcag ttggggcgca ccgttgggag cgggtctcgc agatcttggg gctctgcgag
      181 ccaagcagag aaagaccagt ggcaatcggt gtgggcaggg ttggcgcgcg accatacggt
      241 catccatgcc tgaccattca gttcgttcgg catactgatg tgcggcattg ccggaatgat
      301 gttctgtctg gatgctgagc tcagccctat tgtttcaaaa atggtcgaga tgattcgtta
      361 tcgcggcccc gacgattcag gactgtggtg cgatgccgac tacgggcttg cccttggcca
      421 tgcccgcctg tcgatcttgg atctgtctcc cgccggccat caacctatgg aatcggcttc
      481 cgggcgatat gtcatcgtat tcaacggtga aatctacaat catctggaac tccgagggca
      541 gctcacgggc ctgccgtggc gcgggcattc ggataccgag acgctgttaa cggcctttga
      601 aacctggggt gtggaaaaag cgcttcaggc cactgtcggc atgtttgcat tggctctatg
      661 ggatcgcatt gagcggcgcc tagtcctggc tcgggatcgg atcggagaga agccgctcta
      721 ctatgggtgg tctaacggaa cgttcatctt tgcctcggag ctgaaggcgt tggaagctta
      781 cccgggctgg cgcggtgaga tcgatcggga tgccctggcc ttgttcatgc gctatgctta
      841 tgtgccgtta ccctactcga tctacaccgg aattcgaaag ctcttacctg gaacgtatgt
      901 gacgattcct tccgaatgcc cggcccgcta ttggtctgaa cccaaaccct attggtccgc
      961 ggcggcggtt gccggacagc ccatcgagtc cgagtggaca gatatggcgg ctgaggatga
     1021 actgcatcgg ctgttaagtc gcgcgatcaa gggacagatg ctctcggatg tgtcgctcgg
     1081 cgcgcttcta tcgggcggaa tcgattcttc aaccgtggtg gcgctgatgc aagcccaatc
     1141 ctctcgtccg gtcaagacct tctcgatcgg tttccatgat gatgagtaca atgaggctcc
     1201 gcaggcgaaa gccgtggcca cgcatcttgg aaccgagcat acggagctct atgtgagatc
     1261 ggccgacgcc ttggcggtga ttccgcatct gccgacgatg tatgacgaac cattcggcga
     1321 ttcatccgcc attcctaccc atctcgtcgc gagattggcc aagcaacaga tcacggttgc
     1381 gctttccggt gatgggggcg atgagttatt cgggggctac aatcggtatt cttgggggag
     1441 gtcgatctgg catcgaatcg cgcgagtacc aattcccatg agggcgatga cggcagctgc
     1501 cttgaccatg ctgtcgcccg agcagtggga taggatcggg aggatactga ggccggggct
     1561 tcctgcttcg ctccatatgt cggccctcgg ggagaaggtg cataaacttg ccgcagtcct
     1621 ggattcggat agtcagacgg aattgtatcg gcgacttgtg tcgctacaac gagagacttc
     1681 ttctttggtc atcggcgctc aggacaaacc gatttgggcc gacgctcaag cgcaacaact
     1741 gggggggcgg aatttcagcg aagagatgat gctccacgat ttggtcggat acttgaccga
     1801 cgatatcttg gccaaagtcg atcgggccgc gatggcgatc agtctggaaa ccaggatgcc
     1861 cctgctggac catcgcgttt tggagttttc ctggagtctt cctctttcga tgaagatcag
     1921 ggaagaaaac cagggcaaat ggctgcttcg gcaagtcctg tatcgctatg tcccgaaaga
     1981 tctggttgaa cgacccaaga tgggtttcgg cattccgttg gactcctggc ttagaggcag
     2041 cttgagggac tgggccgagg cgttgttgga ggagtctcga ctcaagcgtg aaggatattt
     2101 cgatccggct ccgatccgag cgaaatggcg ggaacatctc tcgggtcggc gtaatcggca
     2161 gtattggctc tggaacgtct tgatgtttca agcttggttg agtgctcatg agtgatcgcc
     2221 ctcgtctact gtatcttatc actgaagatt ggtatttctg gtcccaccgt gtcgatctag
     2281 cacgggccgc acgggaagcg ggctatgatg tgatcgtggc gactcgtgtg accgatcatg
     2341 gagcgcgaat ccgaggagaa ggattccagc tcgagccgct ggagctggtc cgtcggagcc
     2401 gcaatccgct ccgcgaggcg gtcgccgtcg ccgaactggt gcgattgtat cggcgtgtca
     2461 gacccgatat cgttcaccat gtggccatga aaccgattct gtacggatct ctggtcgcgt
     2521 ggtttacccg agtgccggcg gtcatcaacg cattcgccgg tcttggatat accttc
//
LOCUS       DATNDD010000134         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3228332, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000134 DATNDD010000000
VERSION     DATNDD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3228332"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VN647_03205"
     CDS             complement(<1..1090)
                     /locus_tag="VN647_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HWG96070.1"
                     /translation="MRTETGGATQWDRRRFIAFFLSGLVMTSITQPSPLFAQVLPPIF
                     DPTLRSGEPRAPLKKEFKPPTPPPGLTLPPVPAPPEGDVQKELGQVRVFVNAIQVTGN
                     TVFPDSEIKAVTTPYENRTLTTEDLERLRLALTLLYINNGYITSGAVIPDQDVKDGVI
                     QIQIIEGMLSRIDVEGNNWFRRGYLSDRLMLGAGPPLKIEPLQERLQLLQRDPRLERI
                     NADLRPGDRRGESVLTVKVREARPWKVWLDFNNYQTPIVGAERGLITLAHQNVTGNGD
                     PFSITYGGSRGVHPIIDTSYTLPLNRYDTTFTASYRRNEFVVVSDQFRALDLNSESEI
                     FGFTLRHPIYRSLTDELAVAITGEHLYNK"
     gene            complement(1087..>3202)
                     /locus_tag="VN647_03210"
     CDS             complement(1087..>3202)
                     /locus_tag="VN647_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96071.1"
                     /translation="SIASVASSGEIASGDLMPGSDMRMGPIHLSDSSLLNSSGDAGGS
                     IKIRGGSFVIEESTLSADSSAARGKPIAIDVNLTEDLTITNNSLPALTARAMGEGVAG
                     NIHLNARNISAHTSTDDVTFALIDTHTSGTRNAGAVKITSETLTAQMGTSGAFLIDSG
                     TTSEGNGGTVTINAATVQLKNANVNAGDFRTIVSGLPISSITGLGGNIAILANTISLR
                     STGLASQVFRGHGGDISLIGHQITLNGASFASTTGLSGSGAVLIDAHRFTMTEASQIE
                     ALTAFSSGGDVVMNAATVELSDGSTIRTQTGGPGSAGNIIVKATESVKLSGEEASIRP
                     SGFYSNSLAREGVKVTELGGNAGSISITTPRLAITGGARVDTTTQTSGSGGNVTIKAT
                     DTITIAGQRIVPIPEDFFSVGSDTAGGIFTRTIGGESCSGPCGNGGNISLSARQSISV
                     SDGAVISAASTGSGNAGNIGVDAGQQLNVQNGSITTQADQASGGNIDIRAIDSVRFAN
                     SQISASVGADRGNGGNITIDPKTVVLQNAQILANAQQGNGGNITITTPVFLQDQASRV
                     DASSQFGVNGTITIQSPTSNLSGTVKQLTTKPSETQALLQNRCIALAGGEQSTFIVAG
                     RDALPSEPGGWLSSPLSMDHLIGHGMEHAAGPTAKSVGPSGSSAMVASAGEMQALSLR
                     RLTPPGFLVRTFGSDGLTGCRS"
BASE COUNT          704 a          849 c          863 g          786 t
ORIGIN      
        1 tcttgttgta caaatgctcg ccggtgatcg cgaccgccag ttcgtcggtc aggcttcgat
       61 agatcggatg ccgcagtgtg aagccaaaga tttccgattc ggaattcaaa tccagcgcac
      121 gaaattggtc ggagacgacg acaaattcat tccggcgata ggaggcggtg aaggtcgtgt
      181 cataccggtt caacggcagg gtataggacg tgtcgatgat cgggtgcacc ccgcgcgatc
      241 ctccgtaggt gatgctgaaa ggatctccat tgcccgtgac attttgatgg gcgagggtga
      301 tcagaccacg ctccgctccc acgatcgggg tttgatagtt attgaaatcc aaccaaacct
      361 tccagggacg ggcctctcgt actttcacgg tcaagacgct ttcgccacgc cggtcgccgg
      421 gacggagatc ggcgttgatc cgttcgaggc gaggatctcg ctgcagcaat tgtaatcgct
      481 cttgaagagg ctcgatcttc aatggaggcc ccgcacccag catgaggcga tcgctcaagt
      541 acccgcgccg aaaccagtta ttgccctcaa cgtcgatgcg cgacagcatc ccttcgatga
      601 tctgtatctg aatcacgcca tccttcacgt cttgatcagg aatgacggcg ccggaggtga
      661 tatagccgtt gttgatgtac aagagtgtga gcgccagccg cagtcgttcc aaatcctcgg
      721 tggtcaacgt gcggttttca tacggcgtgg tgacggcctt gatctccgag tccgggaaca
      781 cggtatttcc ggtgacctgg atcgcattga caaatacgcg tacctggccg agttccttct
      841 gaacgtcacc ctccggtggt gccggtaccg gtgggagagt gagacccggc ggaggggtag
      901 gcggcttgaa ttctttcttc agcggcgcac gtggttcacc ggatcggagt gtgggatcga
      961 aaattggggg aagtacttgt gcgaaaagag gcgaaggttg tgtgatactc gtcatgacga
     1021 gtcctgaaag gaaaaaggcg atgaagcgac gccgatccca ttgcgtcgca ccgccggtct
     1081 ctgttctcat gagcggcagc ctgtgagtcc atcactccca aacgtccgaa ctaagaatcc
     1141 gggcggcgtc agccgccgaa gcgacagagc ctgcatctca cccgcagagg cgaccattgc
     1201 cgacgatcca gatgggccga cgcttttcgc tgtcggacca gccgcatgct ccatgccgtg
     1261 tccaatcagg tggtccatcg agagaggact actgagccac ccacctggtt cggaggggag
     1321 tgcgtcgcgt ccggcaacaa taaaggtgct ctgttcgccg ccggccaagg cgatacagcg
     1381 gttttgcaag agcgcctggg tttcgctcgg cttcgtggtg agctgcttca ccgtaccgct
     1441 caaattcgac gtgggcgatt gaatagttat cgtgccgttc actccaaatt gtgatgacgc
     1501 atcgacgcgg cttgcctggt cctgtaaaaa caccggcgtc gtaatcgtga tgttgccacc
     1561 atttccttgc tgtgcatttg cgagaatctg ggcgttctgt agaactaccg tcttggggtc
     1621 aatcgtgata ttgccgccgt tgcctctatc agcaccgaca gaagcgctga tttgactgtt
     1681 cgcgaaccgg acactgtcaa tggctcgaat atcgatattt ccgccactgg cttgatcggc
     1741 ttgcgtcgtg attgaaccgt tttgaacgtt aagttgctgg cctgcatcga ccccaatatt
     1801 acccgcattg ccagaacctg tactagcggc tgatatgacg gccccatcac ttacagatat
     1861 tgactgcctt gcacttaaag agatgttgcc tccattaccg caagggcctg agcaggattc
     1921 gcccccaatc gttcgtgtga aaattcctcc tgccgtatcg ctaccgactg aaaaaaaatc
     1981 ttctggaatc ggaacaatac gttgtcctgc aatcgtaatg gtgtcggttg ccttgatggt
     2041 tacattgcca ccgctcccac tggtttgcgt ggttgtatcg acccgggcgc caccagtaat
     2101 tgcaagacga ggagtagtaa tgctgatgga gccggcattc cctcccaatt cggtaacctt
     2161 tacaccttcc ctggcgagag aattgctata gaaaccgctt ggtcttattg atgcttcctc
     2221 acccgatagt tttacgcttt cggtcgcttt aacaatgata ttgcctgctg atccagggcc
     2281 gcctgtctgt gtacgaatcg tgcttccgtc gctcaattcg acggtagctg cattcatcac
     2341 aacatctcct ccagaggaaa atgcagtaag cgcttcgatc tgtgaggcct cggtcatggt
     2401 gaaccgatga gcatcaataa gcaccgcacc acttcctgat agtccagttg tactagcaaa
     2461 agaggctcca ttgagagtga tttggtgtcc tattagcgaa atatcccccc cgtgacctcg
     2521 aaagacttgt gaggcaagtc cggtggagcg aagcgaaatt gtgtttgcaa gaatggcaat
     2581 attaccgcct aatcctgtga tgctagaaat tgggaggccg gaaacaattg ttcgaaaatc
     2641 gccggcattg acgttggcat tctttaactg cactgtcgcg gcattaattg taacggtgcc
     2701 tccgttacct tccgaagttg tgccactatc aatgagaaat gcaccactag ttcccatctg
     2761 ggccgtgaga gtttctgacg tgatttttac agctcctgca ttcctggttc ctgatgtgtg
     2821 cgtgtcgatt agagcgaatg tgacgtcatc cgtcgaggta tgagcagaaa tatttcttgc
     2881 gttcagatgg atgtttcctg caacaccttc gcccatggca cgagccgtta aggcaggcaa
     2941 cgaattgtta gtaatggtta gatcctcggt caagttaacg tctatcgcga tgggtttccc
     3001 tcttgctgca gagctatctg ctgacaaggt gctttcttcg atcacgaaag atccacctcg
     3061 tattttgata ctgccgcctg catcaccgga agagttaagt aaggatgagt ccgaaagatg
     3121 aataggcccc atcctcatat cagatcctgg catcaagtca ccgctcgcaa tctctccaga
     3181 tgaggctacg ctggcaatgc tt
//
LOCUS       DATNDD010000135         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_591846, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000135 DATNDD010000000
VERSION     DATNDD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591846"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VN647_03215"
     CDS             complement(<1..874)
                     /locus_tag="VN647_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWG96072.1"
                     /translation="MSGLGRFKRLMSYVKPYRIRFVGAFVCSGLVALLSGAYAWLARP
                     VLDDIFIQKDERMLLVLPLALLGVATLKAVFSYGVGYLMAYVGNGVVADIRQQLFQQL
                     MRLSVGFHDSNTSGRLVSRIVNDVGMMANAASSVVKDIFQNGLTFLAMVGVIIYQNWR
                     LAAISLVVIPLSALTMVRVGKRLKRLAASGQEQMGDMASTLQETFAGIRVVKAFGREE
                     AEAERFKERNQTFLSTTLKSNQVWSLGHSHMEIIGVVGVSTIIWYGGYLVIHEVMTPG
                     AFFSFLAAMFMAYTP"
     gene            complement(862..2004)
                     /gene="lpxB"
                     /locus_tag="VN647_03220"
     CDS             complement(862..2004)
                     /gene="lpxB"
                     /locus_tag="VN647_03220"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWG96073.1"
                     /translation="MARILIISGEASGDLHGANLAKALKARDPHVSLAGIGGRAMEAA
                     GVRLVEEMGRFDVIGMVGPFALVSILRRFITMRRLFRSKQWDTVVFIDHPGLNMRFAY
                     FAKAAGLRVVYYIAPQIWAWAPWRIRWIKQRVDHVLVILPFEKSLYEEAGVRCTFVGH
                     PILDAVPGSYDRRGLRSRFGFLDDERVIALLPGSRAHEVQVLLPILLEAAEKLARREP
                     KTKFILAQASTIHDNLLQPLLVRSPVPIALVKEQATEVMALSDLVLVASGTATLQAAV
                     VGTPMVLFYRTTPLTFWFANVVIRFVVRVKWIGLVNLVAGRTVVPELVQSAATGQRLY
                     EEALRILEDRPTYNEMKRDLAKVRAALGEPGASARAAEVVLTACQA"
     gene            complement(2089..3036)
                     /locus_tag="VN647_03225"
     CDS             complement(2089..3036)
                     /locus_tag="VN647_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWG96074.1"
                     /translation="MANLRVGVIGVGHLGQHHARLYASLPDSTLIGVVDHDHGRASLI
                     GGQYGARAFDDLPDLLKQVDVVSVAVPTPAHYSVTKTCLEAGKHVLVEKPIAVLPIEA
                     QELVELAKMNGCTLQVGHSERFNPIMQMMRPHIRRPMLFEGQRMGVYSERGTDVDVVL
                     DLMIHDLDLVLSCHPGPVEEVRAAGLSVRSSTNDVANAYIQFESGCVASLIASRVSLK
                     AMRHMRLFQPDESISMDFQSRQGVIGRRSVEPGRKPMVAVEEFQGGDDEPLRLQLESF
                     LQAIHTQSCPVVSGEAGTAALEMAHRVLSAIKAFGERNS"
     gene            complement(3134..3976)
                     /gene="lpxI"
                     /locus_tag="VN647_03230"
     CDS             complement(3134..3976)
                     /gene="lpxI"
                     /locus_tag="VN647_03230"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HWG96075.1"
                     /translation="MPIPDGRIGVIAGNGRFPIIFADNARKMGLQVFAVAHEGETESE
                     LEQHVDRIHWIKIGQLNKLINAFKSDGVRNVVMLGGIKKTHVYSHARPDFRVLALATK
                     LVLWKDDDILRALAAELEKDGITICESTFGLEGILVEEGTLTSRQPTKKEWIDIRYGW
                     DVAKETGRLDIGQCVVIKDRVVVAVEAVEGTDEAIKRGGELAKDGAVVVKRSKPQQDL
                     RFDLPAVGPRTIEVMRSVKASVLAVEAGRSVMLDRELLISKAEDAGIAVVGLVRETEQ
                     STGT"
     gene            complement(4000..4818)
                     /gene="lpxA"
                     /locus_tag="VN647_03235"
     CDS             complement(4000..4818)
                     /gene="lpxA"
                     /locus_tag="VN647_03235"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWG96076.1"
                     /translation="MKIHPTAIVHPKASLDGDVEIGPFCVIGEHVAIGKGSRLLSHVT
                     IDGWTEIGERNEVHPFASIGGPPQHLGYKGEPTKVIIGHDNIIREYVTMNRGTVQGGG
                     VTSLGSKGILMAYVHVAHDCRLGDHIIMANAASLAGHITIGDHAVIGGLTGILQFVRI
                     GDYVMVGGCCAIGQDIPPFTFAAGGYRAHLYGLNSVGLQRHGFSADRIALLKKAFDLL
                     FRSGHRTAEAIRLAKKEFKGQADVARILTFMEGTKRGISRAVSLDLEREFDQQV"
BASE COUNT         1130 a         1584 c         1238 g         1046 t
ORIGIN      
        1 tcggggtata ggccataaac atcgccgcca ggaacgagaa aaaggcccca ggagtcatca
       61 cctcatgaat gaccagatag cctccatacc aaataatggt cgagacaccg accactccga
      121 tgatttccat gtgcgagtga ccgagcgacc aaacctgatt ggacttcaac gtggtcgaga
      181 gaaaagtctg gtttcgttcc ttgaatcgtt cagcctccgc ctcctcacgc ccgaacgctt
      241 tcacgactcg gatacccgcg aacgtttctt ggagcgttga agccatgtcc cccatttgct
      301 cctgtccgct ggccgccaat cgcttcaatc gtttccccac ccgcaccatc gtcagcgccg
      361 agagcgggat cacgaccagc gagatcgccg ccaatctcca attctgatag atgatgacgc
      421 cgaccatcgc cagaaaggtg agaccgtttt ggaaaatatc tttgacgacg ctcgatgcgg
      481 catttgccat cattcccaca tcgttcacga tccgcgagac cagccgcccc gatgtattgg
      541 agtcatgaaa tccgacggac agccgcataa gctgttggaa caattgctgt cggatgtccg
      601 ccacgacccc gtttccgaca taggccatca aatatcccac gccgtagctg aacaccgcct
      661 tgagtgtcgc gacccctaag agcgccagcg gcagcaccag caacatccgt tcgtcctttt
      721 ggatgaaaat atcgtccaaa accgggcgcg cgagccaggc ataggccccg ctgagcagtg
      781 ccaccagacc tgaacagaca aaggctccta cgaaccgtat acggtacggc ttcacataac
      841 tcatcagccg cttgaatcgc cctaagcctg acatgccgtc aacaccacct ccgcagctct
      901 ggccgacgcg cccggttcac ccaaggcggc ccgaaccttt gccaaatccc gcttcatctc
      961 attatatgtt ggccgatctt ccaagattcg aagagcctct tcatataatc gctgccccgt
     1021 cgcagcggat tgaaccaact ccggcacgac cgtccggccg gccaccagat tgacaagccc
     1081 gatccatttg actcgaacca cgaatcgaat cacgacattc gcaaaccaaa aggtcagcgg
     1141 cgtggttcga tagaacaaca ccatcggagt tccgactaca gccgcttgca gcgtagccgt
     1201 acccgaagcc accagcacaa gatccgacaa tgccatcact tcggtggcct gctccttcac
     1261 cagcgcgata ggaaccgggc ttcgcaccag gagaggctgc aacagattat cgtgtatggt
     1321 ggaggcttgc gccaggataa acttcgtttt tggttcccgg cgtgccaatt tctctgcagc
     1381 ttcgaggagg atcggcagta gaacctgcac ctcatgggcc cggcttcccg gcaacaaggc
     1441 aatcactcgc tcgtcatcca agaaaccgaa tctgctgcga agccccctcc gatcgtagct
     1501 gcctgggacc gcatccaata tgggatggcc gacaaacgta caccgtacgc ccgcttcttc
     1561 atagagggac ttctcaaaag ggagtatgac caggacatga tcgacccgct gtttgatcca
     1621 acgaatccgc caaggcgccc acgcccaaat ctgcggcgcg atgtagtaca ccacccgcag
     1681 gccggccgcc ttggcaaaat acgcaaagcg catgttgaga cccggatggt cgataaacac
     1741 caccgtgtcc cattgttttg atcggaataa ccgccgcatc gtgataaacc gacgtaagat
     1801 cgacaccaat gcgaaagggc cgaccatacc gatgacgtca aagcgcccca tttcctcaac
     1861 caatcgcaca cctgccgcct ccatcgcgcg accaccaatt ccagccaatg agacgtgagg
     1921 atcgcgcgct ttcagcgctt tggcaagatt ggctccatga agatcgccgg aggcttcacc
     1981 ggaaataatc aggatacgag ccatgatgat ctacaagacc ttaggcatga cggcaatctg
     2041 gtgtccgacc gcgaggaacc cgatatggtc agccgacaga agatgttctc aggagttacg
     2101 ctctccgaac gccttaatgg cggacagcac gcgatgcgcc atctccaggg cggccgtccc
     2161 cgcttcgccc gacacgaccg ggcatgattg cgtatgaatg gcctgcagga acgactcaag
     2221 ctgtaatctc aagggctcat catcgccgcc ctgaaactct tcaaccgcca ccatcggttt
     2281 ccgtccaggc tcaaccgatc ggcgtccgat taccccctgg cgggattgga aatccataga
     2341 tatagactca tccggttgaa acagacgcat atgccgcatt gccttcaagg agactcggct
     2401 tgcgatcaag ctggcaacac acccgctttc gaattgaata tacgcgttgg cgacatcatt
     2461 cgtagaagaa cgtaccgaca ggccggcggc tcgtacttct tccaccgggc ctggatggca
     2521 ggacaacacc aaatccaggt cgtgaatcat caaatccaag acgacatcga cgtctgtgcc
     2581 gcgctcgctg taaaccccca tccgctgccc ctcgaacaac atcggccgcc gaatgtgcgg
     2641 ccgcatcatc tgcatgatcg ggttgaaccg ttcgctatgc ccgacttgca atgtgcatcc
     2701 attcatcttg gctaactcca ccagctcctg tgcttcgatc ggcagcacgg ctatcggctt
     2761 ttctaccaga acatgttttc cggcttcgag acaggttttg gtaacggaat agtgtgcggg
     2821 tgtcgggacg gcgacactga cgacatcgac ctgtttcaaa agatccggca ggtcgtcaaa
     2881 cgcccgcgcg ccgtattgcc ccccgatgag agaagctcgt ccatggtcgt gatcgactac
     2941 gccgatcaag gtcgagtcgg gcaacgaggc atacagtcgc gcgtggtgtt gcccgagatg
     3001 tccgaccccg atgacaccga cacgcaggtt ggccatacac tgcttggact gaattgggga
     3061 ctctcttgat cgattaattt gttaagtttc tgaaatcaaa tagatcttca gattccgaaa
     3121 cttcttcgtt gactcaggtc cccgtgctct gctccgtttc gcgaacgagt ccgacgacgg
     3181 cgattccggc gtcttccgcc ttgctgatca gcagttctcg atccagcatc acggaccgac
     3241 cggcctcgac tgcaagcacc gaggccttga cggaacgcat gacctcgatc gttctggggc
     3301 cgacggccgg caggtcaaac cggagatcct gctggggctt gctccgcttc accaccaccg
     3361 caccatcctt ggccagttcg cctccacgct tgatcgcttc atcggttccc tcgaccgctt
     3421 cgaccgccac gacgacacgg tctttaatca cgacacattg gccgatgtcg aggcgccccg
     3481 tctctttggc cacgtcccag ccatagcgga tgtcaatcca ctctttcttg gtcggttggc
     3541 gggacgtgag tgtgccttcc tcaaccaaga ttccttccag gccgaacgtc gactcacaga
     3601 tggtgatgcc atccttttcg agttccgcgg cgagtgcgcg taggatatca tcatctttcc
     3661 agaggaccaa tttcgtggcc aacgccagca cgcgaaaatc aggacgtgca tggctataga
     3721 catgcgtttt cttgatgcct cccaacatga ccacgttccg gacgccgtcg gacttgaacg
     3781 cgttgatcag cttattgagt tggccgatct taatccaatg gatccgatcg acatgctgtt
     3841 caagctccga ttcggtttcg ccctcgtgag ccaccgcgaa tacttggagc cccatcttgc
     3901 gggcgttatc cgcaaagatg atcggaaatc gtccattccc ggcgatgacc ccgattcgac
     3961 cgtcgggtat aggcatggtc aggtgcatag cggcagacat cacacttgtt gatcgaactc
     4021 gcgctccaag tcgaggctga cagcccgcga gattccacgt ttggtaccct ccataaacgt
     4081 gagaatcctg gccacatcag cctgtccctt aaattccttt ttggccagtc gaatcgcctc
     4141 ggccgttcgg tggccggacc gaaagagcag gtcaaaggcc ttcttcagca aggcgatgcg
     4201 atcggctgaa aagccatggc gctgtaggcc gaccgaattc agaccgtaca gatgggctcg
     4261 gtacccgccg gcggcgaacg taaatggagg aatatcctga ccaatggcgc aacaaccgcc
     4321 gaccatcacg tagtctccaa tgcgcacaaa ctgaagaata ccggtcaatc ctccgatgac
     4381 ggcatgatca ccgatggtga tgtgtccggc aaggcttgcc gcattggcca tgataatatg
     4441 gtcgccgagc cggcaatcgt gtgccacatg cacgtaagcc atcaaaatgc ccttcgaccc
     4501 aagagaggtc actccacccc cttggacggt tccccgattc atcgtgacat actcccggat
     4561 aatgttgtca tggccgatga tgaccttggt cggctccccc ttatatccaa ggtgttgtgg
     4621 aggccctccg atcgaagcaa acggatgcac ttcgttccgc tccccgatct ccgtccaccc
     4681 atcgatagtc acatgggaga gcaaccggct tccctttccg atcgcgacat gctccccgat
     4741 tacacagaat ggtccgatct caacgtcgcc gtcgagactc gccttgggat gaacgatcgc
     4801 tgtaggatgt atcttcacgc atgccccctt tactcgcgcc caacatcacc ggtcattggt
     4861 caaccccggg tcactgcctt gcttaccgtg tttctgtctt ctcttccatc accatcgccg
     4921 tcaccacggc ttcacagacc acctcattgt ctacgtatgc cttcccttgc attttccaga
     4981 acggcggacg cttcttga
//
LOCUS       DATNDD010000136         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4164896, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000136 DATNDD010000000
VERSION     DATNDD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4164896"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..436)
                     /locus_tag="VN647_03240"
     CDS             complement(248..436)
                     /locus_tag="VN647_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96077.1"
                     /translation="MAGVKQEVIQLVQSLPETATLDDIMAELYFKMQVDAGLKELDEG
                     AGIEHKDVEKRLGKWLAR"
     gene            complement(455..967)
                     /locus_tag="VN647_03245"
     CDS             complement(455..967)
                     /locus_tag="VN647_03245"
                     /inference="COORDINATES: protein motif:HMM:NF040117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HWG96078.1"
                     /translation="MERQSSERILELLVESDHPPDHQAPTPPKTMNGVYRGILSEIDL
                     ESGLIRIVMPSLFGMKAVSARSIVPISKEKIGEEVLVAFEAGDIRSPYIIGPLWNSQQ
                     PSPEAQSPIEAKVDGEQVVIEGKKEIVLKCGKASITLTRAGKVLIRGAYLLSRSSGVN
                     RIKGGSVQVN"
     gene            complement(980..>2890)
                     /gene="tssI"
                     /locus_tag="VN647_03250"
     CDS             complement(980..>2890)
                     /gene="tssI"
                     /locus_tag="VN647_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HWG96079.1"
                     /translation="CRIFQGKKVPDIIQEIFKENGITDIKTVLKATYEPREYCVQYRE
                     TDFNFVSRLMEQYGMFYFFEHEEKKHTVVLSDDLSAHQLCLEQSKVSWNPQGSDNLNE
                     DVITGLQWEETFRFGQYAVTDYNFETPSTSLHAEVKTQIEVGGNGKYEIYDYPGEYEK
                     KAEGDGIAKIRMQEEEAQYKVMSGSGTARVFTTGYKFTLQDYDARKDVNGDYVLTQVQ
                     HIASVGNTYATEAAGGVENGDYSNSFTCIPAKRSFRPSQVTPKPMVQGPQTAVVVGKS
                     GEEIWTDKFGRVKVQFHWDRYGKMNESSSCWVRVSQNWAGKQWGGVFIPRIGQEVIVE
                     FLEGDPDRPIITGRVYNAEQMPPYALPANQTQSGVKSNSSKGGGGSNELRFEDKKGSE
                     EIYLHGQKDWTIAIENDKNQTVGHDETLSIGNNRTKKVGVDQSETIGANKTINVGANH
                     TESIVANKSMTVGGNHTETISGAEAITVSMASAHTIALARALSIGAGYQVTVGGAMNE
                     SIGGLKAEEIGGLKSVNVIGNSSETTKANKSVDATGNISEKAGKDIAMNAGKNVTTSA
                     TDNISDTAGKDVTIKAGKKMLLDAGDEITIQTGSARIQMKKNGDIVIEGKKISVTASG
                     DIVMKGKKILQN"
BASE COUNT          528 a          864 c          704 g          794 t
ORIGIN      
        1 agaggcactt gttcttccgc gagttctggt tcttggttgg gcttctcagg gaagcggaaa
       61 gtgcctttca ccaccacgac gagggattca cgcccgtcgg gcgccatccc catcgtataa
      121 cccgcttgca tcttggtggc attgagcagt tccatcgtga tcaaagagtg ttgttataga
      181 gtcgtcgcct tacagaacat tctccagtag ccttgcacca gttgcgaaac cgcccgtggc
      241 gaccacctta tcgtgccagc catttcccca gccgcttctc tacgtctttg tgctcgatcc
      301 ctgctccttc atccagctcc ttcaaccccg catccacctg cattttaaag tagagctccg
      361 ccatgatatc gtccaaagtc gccgtctccg gcagcgattg cacgagttgg atgacttctt
      421 gtttcactcc agccatcgtc tcctccagac tgtctcaatt cacttggacc gacccaccct
      481 tgatgcgatt cactcctgac gaccggctga ggagataggc gcctcggatg agcaccttcc
      541 ccgctcgtgt cagcgtgatg ctggccttgc cgcacttcag cacgatttct ttcttcccct
      601 cgatgacgac ctgttctcca tcaactttgg cttcaatcgg tgattgcgct tctggcgatg
      661 gctgttggga attccacagt ggcccgataa tataaggaga acggatatcg cctgcctcga
      721 atgcaaccaa gacttcttca ccaatctttt ctttggagat tggcacgatc gaacgtgcgg
      781 acactgcttt catgccaaac agactcggca tcacaatacg gatcagaccg gattccaaat
      841 cgatctccga caatatcccg cgatagactc cgttcatcgt ttttggcgga gtgggagcct
      901 gatgatctgg tggatgatcg gactcgacaa gcagttcaag tattcgctct gaactctgac
      961 gctccatggt cgtccctctc tagttctgca gaatcttctt gcccttcatg acaatatcgc
     1021 ctgaggcagt gacggaaatc ttcttgccct cgatgacgat gtcgccgttt ttcttcatct
     1081 gaatgcgcgc actacccgtt tggatggtga tttcatcacc ggcatcgagc agcatcttct
     1141 tcccagcctt gatcgtgaca tccttcccgg ctgtatcaga gatgttatcg gtagcgctgg
     1201 ttgtcacatt tttaccggca ttcatggcga tgtctttgcc ggccttttca gaaatgttcc
     1261 cggtcgcatc gacagacttg ttggctttgg tcgtctcgct gctgtttccg atcacgttga
     1321 cggacttcaa gccgccgatc tcttccgcct tgagtccacc gatcgactcg ttcatcgccc
     1381 cgccgaccgt gacttgatac cctgcgccga tgctgagagc ccgagccaat gcgatcgtat
     1441 gggccgaggc catgctgact gtaatggctt ccgcgccgct gatggtttct gtgtgattgc
     1501 cgcctacggt catcgactta ttggcgacga tggattccgt atggttcgct cccacgttga
     1561 tggtcttgtt ggctccaatc gtttcgcttt gatcgactcc gacttttttc gtccgattat
     1621 tgccgatgct gagtgtttca tcatgcccga cggtctgatt cttgtcgttc tcaatcgcaa
     1681 tggtccagtc cttctggcca tgcaggtaga tctcttcgct gcccttctta tcttcaaatc
     1741 gcagctcgtt cgagcccccg cctcctttgg agctattcga cttgacccca ctctgcgtct
     1801 ggttggcggg caaggcatag ggcggcatct gctccgcgtt gtacacgcgg ccggtgatga
     1861 tgggacgatc gggatccccc tccagaaact ccacgatcac ttcctgcccg atgcggggaa
     1921 tgaagacccc tccccattgc ttgccggccc agttctgcga aacccgcacc cagcaggagc
     1981 tgctctcatt cattttgccg taccggtccc agtggaactg gaccttcacc cgaccgaatt
     2041 tatccgtcca aatctcttcg ccggatttcc cgacgaccac ggccgtctga gggccctgga
     2101 ccatgggttt cggcgtcact tgggatggac ggaaggatcg cttcgcaggg atgcaggtaa
     2161 aggagttgga gtagtcgccg ttttctacgc ctcccgccgc ttcggttgca taggtgttcc
     2221 ccaccgatgc gatgtgctgg acctgcgtga ggacatagtc cccattgaca tcctttcggg
     2281 catcatagtc ttgcaaggtg aatttgtagc cggtcgtgaa cacccgcgcc gtgccggagc
     2341 cgctcatcac cttatactgc gcctcttctt cctgcattcg aattttggcg atgccgtcgc
     2401 cctcggcttt tttctcatat tcccccgggt agtcgtagat ctcgtatttg ccgtttccgc
     2461 cgacttcgat ttgggtcttg acttcggcgt gtaagcttgt gctcggtgtc tcgaagttgt
     2521 agtcggtgac ggcgtactgt ccgaagcgga aggtctcttc ccattggagg ccggtgatca
     2581 cgtcctcatt cagattgtcg ctcccctgcg gattccatga gaccttcgat tgttccaaac
     2641 agagctgatg tgccgacaag tcgtccgaca acaccacggt atgtttcttt tcttcatgct
     2701 caaagaaata gaacatgccg tactgctcca tgagacgaga cacgaaattg aaatccgtct
     2761 ctcgatactg cacacaatac tctcgtggtt cgtaggtcgc cttcaacacc gtcttgatgt
     2821 cggtgatccc attctccttg aagatctctt gtataatgtc cggcactttt ttaccctgaa
     2881 atatccggca
//
LOCUS       DATNDD010000137        11522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3248785, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000137 DATNDD010000000
VERSION     DATNDD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248785"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VN647_03255"
     CDS             <1..756
                     /locus_tag="VN647_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWG96080.1"
                     /translation="INRAIEFQGVSLRYENQTAPALTDVDLSIKPGEVVALVGSSGSG
                     KTTLVGLLPRFYEPTGGGIYIDGIPLTSYELQSLRAHIGIVSQEIVLFDDTVRSNIAF
                     GRTGAGQADVEQAAKLAYAHDFILRLPEGYDTIIGERGLKLSGGERQRLAIARAILRD
                     PPILILDEATSALDTESERIVQLALANLMKNRTTLVIAHRLSTIQNADRIVVLDRGAI
                     VEMGSHDELLRQGGVYRRLHALQFQDVTSPHYS"
     gene            753..995
                     /locus_tag="VN647_03260"
     CDS             753..995
                     /locus_tag="VN647_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96081.1"
                     /translation="MNASTATVDTPLQQAWLREQARGQAGRLAARAVDILFQVCLSSS
                     RLRVPVSRGVSAVSSRTVMNNVGLCVKTWGVVSAEC"
     gene            1033..1638
                     /locus_tag="VN647_03265"
     CDS             1033..1638
                     /locus_tag="VN647_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWG96082.1"
                     /translation="MKCSLLPPLGAAAIRSVARTMHCETGGHEAVDGLYREGRSIILA
                     FWHAQQLMIPIGYRGTGSHVLISQHGDGEIIARIIARFGHEAVRGSSTRGGAGALRAM
                     IKLGRSGRDVVVTPDGPKGPRQVAKLGVIHLAKATGLPIVPLAFACSKKNSLRAGIVI
                     WSRTRFPEGYFSTGIRFGSRAKPMKPRSRRLAWSLRRSSID"
     gene            1751..3127
                     /locus_tag="VN647_03270"
     CDS             1751..3127
                     /locus_tag="VN647_03270"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HWG96083.1"
                     /translation="MWKLLYNIGFLLATPVIVGVLLAKPRCRPGLAQRLQLEECLSGL
                     SGRRDRRDRPNRPDRPDQPVIWIHAVSLGEVVAAAPLVKALHERHPEFRYIVTTVTET
                     GREAVEQRLGGIAEHRYAPLDFPWAVAGMVRHLRPMVYVFVETEIWPNLLWTLREQGV
                     PSVLVNGRLSSRSFRRQNVPMIRSFYRSVLRTVTLCLTQSDRDGDRLVALGADPTRVH
                     VTGNIKFDQPLPDVRSDESLRRSFGIDEHEQLILAGSTHAGEEELLVSAYRRIVKTYP
                     ATVLMLAPRHIERAERVEATLREAGLVVQRRSRIREKQAGPRVVILDSRGELSRAYRE
                     AVVAFVGGTLVPVGGHNLLEPAVWGTPVMFGPYTDHCAEVATLLSDSGGGRRVTGEED
                     LVASLDEWLGRPETRYRVGQAARQVVLDNQGALTRSLELIETCLRAAPSYSSSCVTTG
                     PGPLMVKP"
     gene            3129..4220
                     /gene="lpxK"
                     /locus_tag="VN647_03275"
     CDS             3129..4220
                     /gene="lpxK"
                     /locus_tag="VN647_03275"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWG96084.1"
                     /translation="MPFLHPGQPVRKGLYWVAGLYGLIIRFRLWCYRKGWLTTTRLSC
                     RVVSVGNLTVGGTGKTPIVILLTEWLSAKGKKVAILSRGYKRMSKEPRLLVSDGSRIL
                     VGPVEAGDEPFLMAQRCPQAIVAVGADRVALGQWVLEQHPVDCIVLDDGFQHRSLHRD
                     VDLVLLDATDAAGLDALLPAGRLREPLTELCRASAIMITRADSRQDVDAIRSRLQAVS
                     CSSEDAVEVVFRPESLMPIVSGERQPVEWCRRKKAWLVSGIGNSRSFRRSAESMGVEI
                     LGETAFEDHHDYRHDDIEKIRTNLQATGGEIVLTTEKDGGKLLPLLMTSDSWWMLRLG
                     AEVMRGKERLYRLIEESLIGERHGVRARA"
     gene            4198..5904
                     /gene="waaF"
                     /locus_tag="VN647_03280"
     CDS             4198..5904
                     /gene="waaF"
                     /locus_tag="VN647_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWG96085.1"
                     /translation="MGSELVRRNLPRRILVRAPNWIGDAVMCEPAVGGLRSLFPEAEL
                     TMLAKPAVAELFIAHPALHCMLVYEDKGAHAGISGKWSLAGTLRRHRFDLAVLFQNAF
                     EAAFLAWLAGIPRRYGYATDGRVFLLTEPVAVPKDPVPGHQVEYYWNLLRPLGLADGP
                     TLPTLLVSADEDRMADVRLASAGIDSSDLIIGINPGSTYGSAKRWMSDRFAEVAKRLV
                     RRVEQSESVHVAVVILGAKGEEWLGENIAAQLGGRSIVLSGATTIRELMAVLKRCRLL
                     ITNDTGPMHIAAAFGVPVVAVFGPTDWRTTAPYGQERSIVREAVDCAPCLLRECPIDH
                     RCMTRVSVEKVYEAGLSRLSGLFGLSGPDKTDQIDRKILSGYTIFLDRDGTLNPDPGY
                     INSSDQFELFPGVPEALARLKRAGARLIIVTNQSGVARGFVTRDDLDAVHMKLKHLLD
                     PAGASLDAIYFCPHHPDDGCGCRKPNRGMIDQAVREWGVNLERSYLIGDHFRDIELAK
                     RIGARSVLVTTGVVPSQEAESLKASGSPPDWIASSLAEAAEWVLSDARRTSAQIRATV
                     NT"
     gene            6085..7047
                     /locus_tag="VN647_03285"
     CDS             6085..7047
                     /locus_tag="VN647_03285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96086.1"
                     /translation="MSILSNRSRSGKPLRTVICQTCGLVWSDPRPHDARQFYEEEYRL
                     SYKHAYSPKPKHVLRAGHVALSRFEKIERLLSSRKAVLDVGTGGGEFAYLLQSLGHGV
                     SGIEPNRGYADYSIQEYGLTVQVGFVQDATFTPESFDVVTIWHVLEHTEDPGFILALL
                     RSWLKPGGTLVVEVPNVEATCQAPRSTFHEAHLYNFNVVSLRRLAKKQGLYEVKHLIS
                     RDGGNITMFFSRMEPLVEDHYDASIPGNCEWISRIVRRHSNVRRHLTPLPYLRAWQRL
                     CRSLQERRETAGERGGKALLDQLYSPQLQVHSVGHHEEIQIVQG"
     gene            7093..8184
                     /gene="waaF"
                     /locus_tag="VN647_03290"
     CDS             7093..8184
                     /gene="waaF"
                     /locus_tag="VN647_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWG96087.1"
                     /translation="MLEGCPVPFISPLGSRSASALENARILLIKPSSLGDIVHAFPVV
                     SAIKTQWPGSHITWLVKRQWADLVERAEGIDRVWPVEMTIRSWIREGRALRAQRFDIA
                     IDLQGLFRSAIVARFSGAPTRIGFANGREGSPWFYTQRIPVLSPDIHAVDRYLSVAAA
                     LGSSLPGKPRFGLRLLEKDMATVRELCRRKGFSMDKPWVAMNIGARWPTKRWPLASFA
                     AVVDQLHETHRDPVVMIGSADERAYTNRLMALTDRPFVDLSGEIPLGCLPALLSTATA
                     MITNDSGPMHIAAALGVPIITMFGPTSAIRTGPYGAGHHVLAGQVSCSPCFSRVCRHD
                     PELECLHLIKPTQVVDVIRPLLAAHVPCR"
     gene            8175..9200
                     /locus_tag="VN647_03295"
     CDS             8175..9200
                     /locus_tag="VN647_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWG96088.1"
                     /translation="MSMNVLIVRPDGIGDVLLSLPVATQLKRLVPGVTVGFLTSPTVA
                     PLLDRHPDVDYVRTIRFTDPFKKLRLAFSQRVEAAIFLKPFRRLMWAAWVAGVPIRVA
                     TRYRWYSPLANRHIYEHRSEFSKHESEYNVEMLKGLGLRPQPTRPPVLTLTEAERAAG
                     ASQWSGLPRPRVVMHPGGVSARRWRLEQYRDLVVTLTDRGYGVVLTGSDQERTEFGKS
                     LPMSTALPAGVVDLMGHLSLRELMSVIANAHVVVSGATGPAHLAAALGIPTVTLFDPR
                     RNNLPVRWKPLGLGALLRPDVPTCEKCIGEACPYWDCLDRFTVEKVIAAIDKVVENPS
                     PLTVLHI"
     gene            9287..9874
                     /locus_tag="VN647_03300"
     CDS             9287..9874
                     /locus_tag="VN647_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWG96089.1"
                     /translation="MNLQGQRDLLLLTEVERDGTVTQRSLASKLGVALGLTNLYLKRL
                     ARKGYIKITTIPSHRVRYLLTPQGFAEKSRLTYLYMEYSLSHYRDMRARLREALSHAA
                     KNGTKRVVIYGTGELAEMAYLSLREMHMTLIGFIDDNPQEAFLSYPVWQPEALSEWEF
                     DAVLLADMERTVRHREILEQYHVPDMKILALGPSV"
     gene            9928..10434
                     /locus_tag="VN647_03305"
     CDS             9928..10434
                     /locus_tag="VN647_03305"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HWG96090.1"
                     /translation="MSETETGHLRWYALRTKSRHEKFVRDQLNKQGIEPLLPTVKRLS
                     QWKDRKKEIEVPLFSGYCFVRFGKEGKLPVQKVVGVLEVVGSKRPEPIPEEEIESLKT
                     LMTSVLPYDSHPYLHEGMKVEVARGPLQGVHGILLRKEKRHRLVIGVRVIQQAAVVEI
                     DVNDVIAV"
     gene            10537..11013
                     /locus_tag="VN647_03310"
     CDS             10537..11013
                     /locus_tag="VN647_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWG96091.1"
                     /translation="MNKSEICTMYGNSVVEGMRVRVGVGVVVRGPGEAILLEKRRDCG
                     WWGLPGGKVEPGESLIDAAVREVFEETGLTVEVTHLIGVYSDPDGRIVTYPDNGDVVQ
                     LIDVVVGARVVSGHIVCSHESEEVRFFSPSQVPEEIVPPARQPVADAFEGRGGVLR"
     gene            11026..>11522
                     /locus_tag="VN647_03315"
     CDS             11026..>11522
                     /locus_tag="VN647_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96092.1"
                     /translation="MTPYRDLLMLGFGRGLQVLAGLVTIKIATTLLSPGEVGSMNQLM
                     SLAVLGTSALLMPMTAYIGRGCLEWMDAGILSRRLGSYLLIILAVALLYGSAVWVIQI
                     QLTVVSGMTSVWVAGLVVLYTVGFALHTMGASGLNLIGHRFLYILFVNLAAWGGLLLA
                     IWWSK"
BASE COUNT         2351 a         2959 c         3530 g         2682 t
ORIGIN      
        1 atcaatcggg ctattgaatt ccagggagtc tccctgcggt atgagaatca gacggccccg
       61 gctctgaccg atgtggatct cagtattaag cccggcgaag tggttgcgct cgtcgggagc
      121 agcggaagcg gaaagacaac attggtcggt ttgctgccgc gtttttatga accgacgggg
      181 ggcggcattt acatagacgg cattcccttg acctcatacg agttacaatc gctgcgggcc
      241 catatcggaa tcgtctcgca ggagatcgtg ttgttcgatg ataccgtccg aagcaacatc
      301 gcgttcggaa ggaccggtgc aggccaggcc gacgtcgaac aagcggcaaa attggcctat
      361 gcccatgact ttattctccg ccttcctgaa ggatacgata cgatcatcgg ggaacgcggc
      421 ctcaagctat cgggcggcga gcggcaacgc ctggcaatcg cccgcgctat tcttcgtgac
      481 ccgccgattc tgatcctcga tgaagcgaca tcggcactcg acaccgagtc tgaacggatc
      541 gtacagttgg ccttggccaa tttgatgaag aatcgaacga ccttggtgat tgcacatcga
      601 ctctcgacca ttcaaaacgc tgatcgaatc gtcgtcttgg atcgaggggc tatcgtcgaa
      661 atgggctctc atgacgaact gcttcggcag ggaggcgtct atcgccgtct gcatgcccta
      721 caatttcagg atgtgactag cccacattat tcatgaacgc ttctactgca accgtcgata
      781 cgccgctgca acaagcttgg ctacgcgagc aagcgcgcgg ccaagcgggc aggctcgccg
      841 ctcgggcggt cgacatactg tttcaagtat gcctctcttc ctcgcggctc cgtgtgcccg
      901 tttcgcgtgg cgtctcggcg gtttcgtcac gaaccgtcat gaataatgtg ggcttgtgtg
      961 tgaagacgtg gggggtggtg agtgctgagt gctgagggtg ggaacagcct gaagaaacgt
     1021 gttgtatact gggtgaagtg ttcactcctc cctccgctcg gagccgccgc cattcgcagc
     1081 gtcgcgcgca cgatgcactg tgaaaccgga ggccacgagg cagtcgatgg gttgtatcgg
     1141 gaaggacgca gcatcattct cgcgttttgg catgctcagc aattgatgat tccgatcggt
     1201 taccgaggaa ccggttccca tgtgctgatc agccagcatg gcgatgggga aatcatcgca
     1261 cggatcatcg cgcggttcgg ccacgaagcg gtacgtgggt cgagtacgcg tggaggtgcc
     1321 ggtgcgcttc gggccatgat caagctggga cggtcgggtc gggatgtcgt ggtgacaccc
     1381 gacggcccca aggggccgcg tcaggtcgca aagcttggcg tcattcattt agccaaggcg
     1441 acaggtcttc ccatcgtgcc gctggctttt gcctgttcaa aaaaaaactc tttgcgagct
     1501 gggatcgtta tatggtcccg tacccgtttt ccagagggct atttctctac gggaatccgc
     1561 tttgggtctc gcgcgaagcc gatgaagcct cgctcgaggc gactcgcctg gagcttgaga
     1621 cggtcctcaa tcgactgacg gatcaggcgg aacaaaacgt tagtcgttaa tcgtcaagcg
     1681 cggtcccggc gcgaatgctt tcggtcattc tacgtttgac gtatgacgag cggcggtcgg
     1741 tggtgtttcc atgtggaagc ttctctataa catcggcttt cttctcgcta cccctgtgat
     1801 cgtaggcgtt ctcctggcca aaccacgttg tcgaccgggg ttggcgcaga gacttcaatt
     1861 ggaagaatgt ctatctggtc tgtccggtcg acgagataga cgagacagac caaatagacc
     1921 agacagacca gatcaaccag tcatctggat tcatgcggtg tcgctcggtg aagtggtggc
     1981 ggcagcgccg ctcgtgaagg cgctgcacga gcgtcatccg gagtttcgct acatcgtgac
     2041 gacggtaacc gagaccggac gtgaggcggt tgaacaacgt ttagggggca tcgccgagca
     2101 tcgatacgct ccgcttgatt ttccctgggc cgtggccggt atggttcggc atctgcgtcc
     2161 aatggtctat gtgttcgtgg agaccgaaat atggccgaac ttactatgga cgttacgaga
     2221 gcagggtgtg ccctccgtgc tggtgaatgg gcggttgtct tcccggtcgt ttcgacgtca
     2281 gaacgtgccc atgattcggt cattctatcg ctccgtgctc cggacagtga cgctttgcct
     2341 cacgcaatcc gatagggatg gagatcgtct tgttgccttg ggggccgatc cgacgcgggt
     2401 tcacgtgacc ggcaatatca agtttgatca gccgctgccg gatgttcgat cggatgagtc
     2461 tctccgccga agcttcggga tcgatgagca cgaacagttg attctcgccg gcagtacgca
     2521 tgccggcgaa gaggaactgt tggtctcggc ctatcgccgc atcgtgaaga cctatcccgc
     2581 aacggtgttg atgttggcgc cgcgccatat cgagcgcgcg gaacgagtcg aagcgacgct
     2641 tcgagaagca ggacttgtcg tccagcggag gagtcggatt cgagagaagc aagccggccc
     2701 acgcgtggtc atattggata gcagggggga gctgtctcgt gcgtatcgag aagccgtcgt
     2761 ggcattcgtt gggggaacgc tggttcccgt gggtgggcac aatttgttgg aaccggctgt
     2821 gtggggcaca cctgtcatgt ttgggcccta tacggaccat tgtgctgagg tcgcgacgct
     2881 cttatccgat tccggaggag gccgccgtgt gacgggagag gaggatctgg ttgcctccct
     2941 tgacgaatgg ttggggcggc ctgaaactcg atatcgggtg ggacaggctg caagacaggt
     3001 ggtgttagac aatcaaggcg cgctcacacg gagtctggag ctcattgaaa cctgtctgcg
     3061 tgcggctcct tcgtattcta gttcatgtgt aacaacgggg ccgggacctc ttatggtcaa
     3121 accctaacat gccattcttg catcctggac aaccggtccg taaagggctt tactgggttg
     3181 ccgggttgta cggtctcatt atccgatttc ggctgtggtg ctatcggaaa ggttggctta
     3241 cgaccacacg gctgtcctgt cgagtggtga gcgtgggtaa tcttacggtc ggggggaccg
     3301 gaaaaactcc catcgtcatt ctcctgacag aatggctgtc ggctaaaggg aagaaggtgg
     3361 cgatcttgag ccgggggtac aaacggatga gcaaggaacc tcgccttctc gtgtccgatg
     3421 ggtcgcggat tttggttggt ccggtagaag ccggggatga gccgtttctc atggcacaac
     3481 gttgtccaca ggcgattgtc gcggtgggag ccgatcgggt tgccctagga caatgggtat
     3541 tggaacagca tccagtcgac tgcattgttt tggatgatgg atttcaacat cgatcgcttc
     3601 atcgtgatgt cgatcttgtg ctgctggatg ccaccgatgc cgcaggcctt gacgcgctgc
     3661 ttcctgccgg aagactacgg gaacccttga ccgagctgtg tcgagcgagt gcaatcatga
     3721 tcacccgtgc cgattctcgt caggatgtgg acgcgattcg tagccgattg caagccgttt
     3781 catgttcatc agaagacgcg gttgaagttg tgttccgacc ggagtccctt atgccgatcg
     3841 tttcgggaga gcggcaaccg gttgaatggt gccgaaggaa aaaggcttgg ttggtcagcg
     3901 gcatcgggaa tagccgatca ttccgtcgat cagccgaatc catgggggtt gagatcctgg
     3961 gtgagaccgc ctttgaagac catcacgact atcgccacga tgatatcgag aagattcgca
     4021 ccaatctaca ggccaccggc ggcgagatcg ttctgacgac tgaaaaggac ggtgggaagc
     4081 tgcttccctt gctcatgaca agcgactcct ggtggatgct tcgtcttggg gcggaagtca
     4141 tgcgggggaa agagcggctg tacagattga tcgaggagtc gttaatcggt gagagacatg
     4201 gggtcagagc tcgtgcgtag gaatttgccc cgcagaattc tggtacgtgc gccgaattgg
     4261 atcggcgatg ccgtcatgtg cgaaccggcg gtcggtggcc tgcggtctct gttccccgag
     4321 gcggagttga cgatgcttgc caaacccgcc gttgcggagc tattcatcgc ccatccggct
     4381 cttcattgca tgctggtgta tgaggacaag ggggctcatg cggggatatc gggaaagtgg
     4441 tcgctcgccg gtacgttgcg acggcaccgc ttcgatttgg cggttctgtt tcagaatgcg
     4501 ttcgaagccg catttcttgc gtggctcgcc ggcatcccac ggaggtatgg gtatgccacg
     4561 gacggaagag tcttcttgct caccgagccg gtcgctgttc ccaaggaccc tgtgccgggg
     4621 catcaagtcg agtactattg gaatttattg agaccattgg gactggctga tggccccact
     4681 ctccccacgc tgctcgtttc ggcggacgag gaccgaatgg ccgacgtacg gcttgcctcc
     4741 gccggtatcg attcgtcgga tctcattatc ggcatcaatc ccggatcgac ctacggcagc
     4801 gcaaaacgtt ggatgtccga tcgatttgct gaagtcgcga agcgacttgt gcggcgagtg
     4861 gaacagtctg aaagcgtgca tgtcgcagtg gtcatcctgg gagcgaaggg agaggaatgg
     4921 ctgggagaaa acattgcggc tcagctcggc gggcgatcga tcgtcttatc cggcgcgacg
     4981 accattcggg agctcatggc ggtgctgaaa cgttgtcgct tgctgattac gaacgataca
     5041 ggaccgatgc atattgcggc ggcgttcggt gtgccggtgg ttgcggtgtt cggtcccacg
     5101 gactggcgca ccaccgctcc ctacgggcaa gaacggtcga tcgtgcggga agccgtagac
     5161 tgtgcgccct gcctcctcag agaatgcccg atcgatcacc gttgcatgac aagggtttcg
     5221 gtggaaaaag tctatgaagc cggtctgtct cgtctgtctg gtctcttcgg tctatctggt
     5281 ccagacaaga cagaccagat agaccggaaa attctttccg gttacaccat ctttctcgat
     5341 cgagatggaa cgctcaaccc tgatcccggc tacatcaact cctccgatca gtttgagctg
     5401 tttccagggg tgcctgaggc gctcgccagg ctgaagcggg ccggtgctcg attgatcatt
     5461 gtcaccaacc aatcgggagt cgcgcgagga ttcgtgacgc gcgatgacct cgatgccgtg
     5521 cacatgaaac tgaagcatct cctcgatccc gccggtgcgt cgctcgacgc gatctatttc
     5581 tgtcctcacc atcctgacga cgggtgcggt tgtcgaaagc cgaaccgtgg gatgatcgac
     5641 caagcggtgc gagaatgggg agtgaatctt gagcgatcct atctgatcgg cgatcacttc
     5701 cgcgatatcg agttggcgaa gcggattggg gcgcgaagtg tcttggtgac gacgggagtc
     5761 gttccctcac aagaagcaga aagtctgaaa gcctcggggt cacctccaga ttggatcgca
     5821 tcctcgctgg cggaggccgc cgagtgggtg ttgtccgatg caagaagaac gtccgcacag
     5881 atacgcgcaa ccgtaaacac gtgatggaga tggaaaggct gtgtccggcc cggcttgtgt
     5941 ctgccggaga accggcgtaa gttttgtcgg tagaatgcga cgcgcaaagg agtcggctgt
     6001 gttgctcaga gagagagaaa gatggtagtg agccggcaag taaacccttg ttccattcct
     6061 tgtaatcttt gtggcggcac cgaggtgtcg attctttcga acagaagccg aagcgggaag
     6121 cccttacgaa ccgtcatttg tcaaacctgc gggctcgtgt ggtcggaccc gcgccctcat
     6181 gacgccaggc agttttatga agaggaatat cgcctctcct ataaacacgc ctacagccca
     6241 aaaccgaagc atgtgttgcg cgcgggccat gtggccttat cgcgattcga aaagatcgaa
     6301 cggttgctgt cgagtcggaa agccgttctc gatgtcggca cggggggggg agagtttgcc
     6361 tatctgcttc aatcgttggg acacggtgtg agcgggattg aaccgaatag agggtatgcc
     6421 gactattcca tacaggaata tgggctcacg gttcaggtcg ggttcgtgca ggatgcgacg
     6481 tttacaccgg aatcgtttga tgtggtcacg atctggcatg tcctcgaaca tacggaagat
     6541 cctggtttca ttttagcctt gcttcgatcc tggcttaaac ctggcggcac gctcgtggtg
     6601 gaagttccga atgtggaggc gacgtgccaa gctccgcgca gcacgttcca tgaggcccat
     6661 ctgtataatt tcaatgttgt gtcattgcgc aggttggcca agaaacaggg gttgtatgag
     6721 gttaagcatc tgatctctcg cgacggtggg aacatcacga tgttcttttc tcgcatggag
     6781 ccgctggtcg aggatcatta cgacgcctcg attcccggca actgcgaatg gatctcaaga
     6841 atcgtgcgtc ggcacagcaa tgtgcgacgc cacttgacgc cgctgccgta tctacgggct
     6901 tggcagcgcc tctgccgatc acttcaagag aggcgcgaga cggcgggcga gcggggcggc
     6961 aaggcactgc tcgaccagct ctattctcct cagctgcagg ttcattccgt agggcaccat
     7021 gaagagattc agatcgtaca aggatgaatg atacgcgttc taccttcgtc tccaatttct
     7081 gacgagcggg gcttgctgga aggttgtccg gtgccgttta tcagccctct cggtagccgt
     7141 tccgcctcgg cgttggagaa cgcccgtatc cttttgatca agcccagttc tcttggagat
     7201 atcgttcatg cgtttccagt cgtctcggcg atcaaaacac aatggccggg atcgcatatt
     7261 acgtggttgg tgaagcgcca atgggccgac cttgtcgagc gggcggaagg aatcgaccgt
     7321 gtctggcccg tagagatgac gataaggagt tggatcagag aaggccgggc gttgcgtgca
     7381 cagcggttcg acatcgcgat agatttgcag gggctgtttc gcagtgccat cgtggcacgc
     7441 ttcagcggcg caccgacgcg tatcggattc gcgaatggga gagaaggaag cccatggttc
     7501 tatactcaac gcataccggt tctcagccct gacatacacg ccgtcgaccg atatctttca
     7561 gttgccgcgg cgttgggatc atctttgccg ggaaaaccgc gattcgggct caggttactg
     7621 gagaaggaca tggcgaccgt tcgggagctc tgccgacgca aaggcttttc aatggataag
     7681 ccatgggtgg cgatgaacat tggcgcacgc tggccgacga aacgctggcc attggcgtct
     7741 ttcgccgccg ttgtggatca gctgcacgaa acacatcgtg atccggtcgt catgatagga
     7801 agtgcggatg agcgagcata cacgaatagg ttgatggctc tgacggaccg tccgttcgtc
     7861 gatctgagtg gtgaaattcc attagggtgc ttgcccgccc tactctcgac cgcaaccgcc
     7921 atgatcacaa acgattccgg accgatgcac attgctgccg cgcttggagt tccgatcata
     7981 acgatgtttg gaccgaccag cgcgattcgg accggtccgt atggggccgg acatcatgtc
     8041 ttggccggtc aagtatcctg tagcccttgc ttcagtcgtg tgtgccgaca cgatcctgaa
     8101 ttggaatgcc ttcatctcat taagccgacc caagtggtcg atgttatccg gccgctgttg
     8161 gcggctcatg taccatgtcg atgaatgttc tgatcgttcg cccggacggc atcggcgacg
     8221 tcctgctctc ccttcccgtg gcgactcaac tcaagcggct tgtgcccggg gtcacagtcg
     8281 ggtttctcac cagtcccacc gtcgcacccc ttctcgatcg ccatcccgac gtggactatg
     8341 tgcgaacgat tcgcttcaca gatccattca aaaaactccg tctcgccttt tcacagaggg
     8401 ttgaggcggc gatttttctg aagccgtttc gtcgacttat gtgggccgca tgggtggcgg
     8461 gtgtgccgat tcgagtggca acgcggtacc gttggtatag cccgttggcc aatcgccata
     8521 tctatgaaca ccgcagtgaa ttttcgaaac atgaatcaga gtacaacgtc gaaatgttga
     8581 agggcttggg attgaggccg cagcctacac gcccgccggt gctcactctg accgaggctg
     8641 aacgcgccgc cggtgcgtcc caatggtcgg gattgccgcg tcctcgcgtc gtcatgcacc
     8701 cgggaggagt gtctgctcgc cgctggcggc tggagcagta ccgtgatctg gtcgtgacgt
     8761 tgacggacag aggctatggg gtcgtgctga caggcagcga tcaggaacga acggagttcg
     8821 gaaaaagttt accgatgtct acggcgcttc ccgcaggggt agtggatctt atgggtcatc
     8881 tgtcgctgcg agaactgatg tcggtaatcg ccaatgctca tgtcgtcgtc tcaggagcga
     8941 caggaccggc tcacctggct gcagccctgg gtatccccac cgtcaccttg tttgatcctc
     9001 gacgaaacaa tctgccggtg agatggaaac cgcttgggct gggagcattg ctgcgccccg
     9061 acgttcccac ttgtgaaaaa tgtattggag aagcctgtcc ttattgggat tgtcttgatc
     9121 ggttcactgt cgagaaggtg atcgccgcaa tcgacaaggt cgtggagaat ccttctccac
     9181 tcaccgttct tcacatataa gtcagagatc aaggataact ctcctggtcc actcttgact
     9241 ccaattcgct tctgtgttca tattctgaac gtttggttct aaccatatga atctccaagg
     9301 acaacgagat cttctcctgc tgaccgaggt agagcgagat ggtacggtta ctcagcggtc
     9361 tctcgcttca aagttagggg ttgctctggg cctgaccaat ctctatctta agcggttggc
     9421 acggaagggt tatatcaaga taaccacgat tccctcacac cgggttcggt atttactgac
     9481 cccgcaaggg tttgccgaga aatccaggct gacctacctc tatatggagt attccctgtc
     9541 tcactaccgc gatatgcgtg cgcggttacg tgaggcactg tcccatgcgg ccaaaaatgg
     9601 aacgaaacga gtcgttatat atggaaccgg tgaattggca gagatggcct atctgtcgct
     9661 ccgagaaatg catatgactt tgattggatt tatagatgat aacccgcagg aggcgttttt
     9721 atcctatccg gtctggcagc cggaggcgtt aagtgaatgg gagttcgatg cggtattgct
     9781 ggcggatatg gaacgaacgg tacggcaccg cgaaatattg gagcagtatc acgttccaga
     9841 tatgaaaatt ctggcgctgg gcccgtcggt ctaagcgttt gaagggtaat cgatttttcc
     9901 gttcactgta agtgagaggg cgaagctgtg tccgagaccg agacaggcca tctccgctgg
     9961 tacgcgcttc gtacgaaatc tcgccatgag aaattcgtac gggatcagct taacaaacag
    10021 ggcatcgaac ctctgttgcc gacggtgaaa cggctcagtc agtggaagga tcgcaaaaaa
    10081 gaaatcgaag tgccgttatt ttccggctac tgttttgtgc gattcggaaa agagggaaag
    10141 ctgccggtgc aaaaagtggt tggagttctc gaagtcgtcg gcagcaagcg accggagccc
    10201 attcccgagg aggaaatcga atcgttgaag actttgatga ccagtgtcct tccttatgat
    10261 tcgcatccgt acctacatga gggtatgaag gtagaagtcg ctcgtggtcc cttacaggga
    10321 gttcatggaa ttctcttgcg gaaggaaaaa cgccatcgac tcgtgatcgg agttcgagtg
    10381 atccagcagg cggcggtggt agaaatcgac gtgaatgacg tcattgccgt gtgagaagcc
    10441 tgtaaccggc tcgatacctc ttccgccgga atgaagtgag ccgtcttgtt acctgctcac
    10501 ccgaacccta aagaacatga tgttggaaga atctgcatga acaagtccga gatttgcaca
    10561 atgtacggga attccgttgt agaggggatg cgggtccgcg tgggagtcgg ggtggttgtg
    10621 cgaggcccgg gagaagccat cctgttggaa aagcgtcgcg actgtggatg gtggggatta
    10681 cctggaggaa aggtcgagcc gggtgagtct ctcattgatg cagccgtccg agaggtgttt
    10741 gaagagaccg gcctcactgt agaggtgacg catctgatcg gcgtctattc cgatcctgat
    10801 ggccgtatcg tgacctatcc ggataatggc gacgtggttc agttgatcga cgtcgtcgtt
    10861 ggagcgcggg tggtgtccgg acacatcgta tgcagccatg agagtgaaga ggtacggttt
    10921 ttctctccat ctcaagtgcc tgaagaaatt gtcccgccgg cgcggcaacc ggttgccgac
    10981 gcatttgaag gccgaggcgg agtcctgcga tagagtgggc ttgccatgac accgtaccgt
    11041 gacctcctca tgcttggttt tggacgaggg cttcaagtcc ttgcaggcct ggtcaccatc
    11101 aagattgcaa cgacgttgtt gtcacccggc gaagtcggaa gtatgaacca gcttatgagc
    11161 cttgccgtcc tggggacgtc ggccttgttg atgcccatga cggcctatat cggccgtgga
    11221 tgtctggaat ggatggatgc cggaatatta agtcgacggt tgggctccta cctgctcatt
    11281 attttggctg ttgctctgtt atatggttcg gccgtttggg tcattcagat tcagttgacg
    11341 gtggtttcag gaatgacttc agtttgggtc gccggattgg tcgtgctcta tacggtcggt
    11401 tttgcactac atacgatggg ggcttcaggg ctgaatctta taggccatcg atttctctat
    11461 atcctgttcg tgaacctcgc ggcgtgggga ggtcttctcc tggccatatg gtggtcgaaa
    11521 ca
//
LOCUS       DATNDD010000138         2888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1291236, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000138 DATNDD010000000
VERSION     DATNDD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291236"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1510
                     /locus_tag="VN647_03320"
     CDS             50..1510
                     /locus_tag="VN647_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96093.1"
                     /translation="MNPFLTRLAHGLISPLPFLAVLVLSSGCGPSVNRYLLIEQSLVA
                     GDPQRAAAIVEQTEKEYGAKGRLLYEMDRGMVLQLAGQYQRSSATLERAEEEIERLYT
                     RTIRSETAAFLTSDNALPYEGDAYEHVMINVIKALNYAAQGQLQEALVEARRIDHRLN
                     VLSDRVTDADKYRNDGFARYVSGILYEATGDLNNAFIAYRNAYEAYESMQRWSKMSSP
                     PSLRSDLLRTAEALALTTEFAEYRQAFPDVEWQSSSSQERLAQVVMVSYNGRAPRKED
                     LFLDLPISLDALQLVLINRGVFQSPYQRNRAVDSVLYGLNGQVVRVALPRLVPQKTQV
                     AFENMTLTDSGSHSVSVRSELAHSVTALAQKSLSERLPAITVKALARAATKYAMAEGI
                     TRGAQQAAGRDGAPWVGLLVALVTHGLAVASEEADKRSWQTLPDEIHVARAWVSPGQY
                     QMSIRPSGQGVTTAKDGQTITLTAGQTMFIIQRVMQ"
     gene            1507..2646
                     /locus_tag="VN647_03325"
     CDS             1507..2646
                     /locus_tag="VN647_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HWG96094.1"
                     /translation="MMRAGQPSVGQGIAVFLCLWLTMSAVTACTWFTGKGKPGWVDGR
                     SAEYPSIQYLTGVGQADTRSNAEDQAYAAVARIFKAEVAAQAKDWESYWVVENHGVSN
                     AERRLTIDNVTKVSTDKVLENVRIADVWYDSDSRSYHALGVMNRSQAEAALMEKVSAS
                     DRAIDADVTESRQTTDKLAKVRALRRAGRNLVLREVYNTDLRVIRPSGQGTPSVYQVN
                     ELSGELEQFLATNLVIAVQVSGDHAEPVGRALEEGLIREGLQVTTKPEGEGTGSPEVI
                     VRGTVRLFPVEVRDPNFKYVRWCSDFEVVEPGTGRILGAVAQGGREGHLTEREATAKT
                     LRVMQQELSSDVAKAIAAHIFGEVSLPETATMPAGCPREESGVKR"
     gene            2684..>2888
                     /locus_tag="VN647_03330"
     CDS             2684..>2888
                     /locus_tag="VN647_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96095.1"
                     /translation="MKKTDELSVNRSPRSRGRGLTKSGQKVSSRLAKRRLNDRLKEDT
                     SSFNRGNLADTLRKEGYEEVPLDE"
BASE COUNT          649 a          743 c          907 g          589 t
ORIGIN      
        1 gaaggtggtt gaaaggaaac gcaccatctt ttaggttccg cggacgctgt tgaacccatt
       61 cctcacacgc cttgcccacg ggcttatctc gccgctgccg tttcttgccg ttctcgtgct
      121 ctccagcggg tgtggccctt ccgtcaatcg gtacctcctc attgaacaaa gccttgttgc
      181 cggtgatccg caacgggccg cggcaatcgt cgagcaaacc gagaaagagt atggagccaa
      241 ggggcggtta ctgtatgaaa tggatcgggg aatggtgctg cagctggcag gccagtatca
      301 gcgaagcagt gcaacattgg aacgagcgga agaggagatc gagcggctct ataccaggac
      361 catccgttcg gaaaccgccg cgtttctcac cagcgacaat gccttgccgt acgaaggcga
      421 cgcttacgag cacgtcatga tcaacgtaat caaggctttg aactatgcgg ctcagggaca
      481 acttcaggaa gcattggtgg aggcgcgacg tatcgaccac cgcttgaatg tgctcagcga
      541 cagagtgacg gacgccgata agtaccggaa cgatgggttt gcgaggtatg tgagcggtat
      601 tctatacgag gcaaccggcg atctcaataa tgcgtttatc gcctatcgca acgcatatga
      661 agcgtacgaa tcgatgcaaa ggtggtcgaa gatgtcctct cctccgtccc tgcgctctga
      721 tctcttgcgc acagcggagg cgcttgcctt aaccacagag tttgccgagt atcgacaggc
      781 tttccctgac gttgagtggc agtccagctc atcccaagaa cggctcgccc aagttgtgat
      841 ggtcagttat aatggtcggg ctccacgcaa agaggatctg ttcttggatc tacccattag
      901 cttggatgcg ctgcagttgg tcttgatcaa tcgtggagtc ttccagtccc cctatcagcg
      961 aaatcgcgca gtggacagtg tgctctatgg actcaacggt caggtcgtcc gcgttgcgct
     1021 cccgcggtta gtcccgcaaa aaacacaggt ggcgtttgaa aatatgacac tgaccgattc
     1081 gggcagccac tcggtcagcg tacgatcgga actcgcgcat agtgtgacgg ccctcgcaca
     1141 gaagagcctt tcggagcgct taccggcgat caccgtgaaa gctctcgctc gagccgcgac
     1201 aaaatatgcg atggcggaag gcatcaccag aggcgcgcag caggcggctg gaagggatgg
     1261 cgctccctgg gttggactgc tggttgcgtt ggtgactcat gggctggccg tggcgtccga
     1321 agaagccgac aagcgaagtt ggcagacctt gcccgacgaa attcatgtgg ctcgggcgtg
     1381 ggtatcgccc ggccagtacc agatgtccat tcgaccgtcg gggcaagggg tgacgacggc
     1441 caaggatgga caaacgataa cgctgactgc aggccagacg atgttcatca tccagcgtgt
     1501 gatgcaatga tgcgtgccgg tcaaccttct gtcggacaag gcatcgcggt tttcctgtgt
     1561 ctgtggttga cgatgtccgc agtgacggcc tgtacctggt ttaccggaaa agggaagccg
     1621 gggtgggtcg atggccggag cgcggaatat ccatcgattc aatacctgac cggggtcggc
     1681 caagcagaca ctcgaagcaa tgccgaggat caggcctatg cggctgtggc ccgcatcttc
     1741 aaggcggaag ttgcagcgca ggctaaagac tgggaatcgt attgggtcgt tgagaatcac
     1801 ggtgtctcca acgccgaacg gcggctcacg atcgacaacg tgacgaaggt atcgaccgat
     1861 aaagtgcttg aaaacgtcag gattgcggat gtgtggtacg actcggacag ccggtcgtac
     1921 cacgctctgg gcgtgatgaa tcgctctcaa gccgaagctg cattgatgga gaaggtgtcg
     1981 gcgtcggatc gcgcgatcga cgcggacgtc accgagtcac ggcaaacgac cgacaaactt
     2041 gccaaggttc gtgctctgcg gcgggccggc agaaatctcg tgttgcgcga ggtctacaac
     2101 acggatttac gggtgatccg tcccagcggg caaggtactc catccgtcta tcaggtaaac
     2161 gaattgtccg gcgagttgga gcaatttctt gctacaaatc tggtgattgc ggtacaagtg
     2221 tccggcgacc atgcggaacc ggttggacgt gctctggagg aagggctcat ccgggaagga
     2281 cttcaggtca cgacaaagcc ggaaggtgag gggactggct cccctgaagt gatcgttcga
     2341 ggcactgtcc gtctgttccc cgtcgaggtg cgggacccga atttcaagta cgttcggtgg
     2401 tgtagtgatt tcgaggtcgt ggagccgggg acggggcgta tccttggggc ggtggcgcag
     2461 ggtggaaggg aaggacattt gacggagcgt gaagcaacgg ccaaaacttt gcgtgtgatg
     2521 cagcaagagc tgtcgtcgga tgttgcaaag gcgattgccg ctcatatctt cggcgaagtc
     2581 tcgctgcctg agacggccac gatgccggct ggttgtccac gagaagaatc aggagtgaag
     2641 cggtagcggt aatgaacgtt ctttcatgag gaggcctttt atcgtgaaaa agacagacga
     2701 attatccgtc aaccgatctc cacgcagcag ggggcgggga ctgaccaagt caggacaaaa
     2761 ggtatcaagc cgtttggcca agcgccgatt gaacgaccga ctgaaggaag acacatcgtc
     2821 tttcaatcga ggcaatctcg ccgacacctt gcgaaaagaa gggtatgaag aggtgccgct
     2881 cgatgagc
//
LOCUS       DATNDD010000139         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_812266, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000139 DATNDD010000000
VERSION     DATNDD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812266"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VN647_03335"
     CDS             complement(<1..542)
                     /locus_tag="VN647_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96096.1"
                     /translation="MAPRPARFDRDAFHGKVEPGESMLSVDHQVLSLRLAQVADGIRA
                     IYLLEAKCFIGKEKHRAGNHRLRHDSLIKVDDLFDLLPIQDPLESLLASLDSRNKLRH
                     IVMVRNLRLCDLFTLKVVSAGEPNLVQELTGLVRDEIKCALFLRNPRREHGASMGLNR
                     SRLKDSTRPSPWPQDKGLCY"
     gene            548..1198
                     /locus_tag="VN647_03340"
     CDS             548..1198
                     /locus_tag="VN647_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96097.1"
                     /translation="MFDGRSADFATEMFVIINSTHTRINRSHLVDLYEKVSWESPEKK
                     FAAKVVNLLYGEPDSPLQYKINRLGGRSKQEKWILQSEVFNELLKVVTVHKRWMESRL
                     GMKADRCYALVRDYLKGVKDVMGEVWGQNDRYMFTRDVTLKALIRVLDDLIMDRKLIN
                     DWDEQRSHKPFAEIVKPWAPLTKEFRADGFYERFPAKGQLERVRKIHQRLLDAIVG"
     gene            1182..1775
                     /locus_tag="VN647_03345"
     CDS             1182..1775
                     /locus_tag="VN647_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWG96098.1"
                     /translation="MRLSDKSQEAWIILLLLLVVQGPLSIVVLGSSALAAADADGLEV
                     KTEPGGGIRATAHPIFPAKPEIIQTLLADYAHWPDLFEVRMRVAELHIHDGIATVDLR
                     IDHPLMPGERRLITESRILPNGGLVTDLTGGDFKRYHRVWKLQPAGEGNQTGADFELV
                     VEIASMVPDWLVAIAIRQELEMHFRIVRQKALEHSKR"
     gene            1781..>2258
                     /locus_tag="VN647_03350"
     CDS             1781..>2258
                     /locus_tag="VN647_03350"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWG96099.1"
                     /translation="MAQVSGEKHPSASILAGLYVILDPSVSSDRPLVDVLNISATAGA
                     KIFQYRNKTASMKAAYAEALPLRKAAHELGVLLIVNDRCDLALAVDADGVHLGQGDLP
                     LNLARKVMGPDKLIGISTHSREQVLAATAGGPNYLGFGPIFMPGSKLDHDLVVGLQG"
BASE COUNT          525 a          559 c          670 g          504 t
ORIGIN      
        1 tggtaacaca atcctttatc ttgcggccac ggagagggcc gcgtagaatc tttcagccgg
       61 cttctattca aacccatgga ggctccatgc tcgcgacgcg gattacgcaa aaagagggcg
      121 cattttattt catcgcgtac aaggccggtg agctcttgaa caaggttcgg ttcaccagcc
      181 gatactactt tgagggtgaa gagatcgcac aggcgaagat ttctgaccat gacgatgtgg
      241 cgcagtttat tgcgggaatc gagcgaagcg agaagggatt ccaacgggtc ttgaatcggc
      301 agaaggtcaa acagatcgtc aacttttatg agactgtcgt ggcgcaaccg atgattcccg
      361 gcacggtgct tctctttacc gatgaaacac ttcgcttcca aaaggtagat ggctcggatt
      421 ccatcggcca cctgagcgag ccgaagggaa agtacctggt gatcgacgga caacatcgac
      481 tcgccgggct ccacttttcc atgaaaagca tcccgatcaa agcgcgcagg tcgaggtgcc
      541 atgcctcttg ttcgatgggc ggagcgcgga cttcgccacg gagatgttcg tcatcatcaa
      601 ttccacccac acgcggatca accggtcgca tttggtcgat ctctatgaga aagtctcatg
      661 ggaaagtccc gaaaagaaat tcgcggcgaa agtcgtgaac ctcctctatg gtgaaccgga
      721 ctcgccgctg cagtacaaga tcaatcgcct tggagggcga agcaaacagg aaaaatggat
      781 tctgcagtcc gaggtgttta acgaactgct gaaagtcgtg acggtccata aacgctggat
      841 ggagtcccgc ctggggatga aggccgatcg ctgttacgcg ctcgtgcgtg actacctgaa
      901 gggggtcaag gacgtcatgg gcgaggtctg ggggcagaac gaccggtata tgtttactcg
      961 cgatgtgaca ctcaaggcgt tgattcgcgt cctggacgat ctgatcatgg atcgcaagct
     1021 catcaatgac tgggacgaac agcgctcgca caaaccgttt gccgagatcg tcaaaccctg
     1081 ggcacctctg acgaaggagt tccgggctga cggcttttac gaacgctttc ctgctaaagg
     1141 ccaacttgaa cgagtgcgga agattcatca gcggctgctg gatgcgattg tcggataaat
     1201 cgcaagaagc ttggatcatt cttctgcttc tgctcgtcgt gcagggcccg ctgtcgattg
     1261 ttgtattggg atcatcggct ttggccgctg ctgatgctga tggattagag gtgaagacgg
     1321 aacctggtgg tggaattcgt gcgacggcac atccgatttt cccggccaaa ccggaaatta
     1381 tacaaacgtt gctggcggat tatgcgcatt ggccggacct gtttgaggtt cgaatgcggg
     1441 tggccgagtt acacatccat gatgggattg cgactgtcga ccttcgcatc gatcacccct
     1501 tgatgccggg cgagcgcaga ttaatcacgg aatcaagaat cttgccgaac ggagggcttg
     1561 tgaccgacct cacgggaggc gattttaagc gctatcatcg agtgtggaaa ctacaaccgg
     1621 cgggggaagg gaaccagaca ggtgctgatt tcgaactggt ggttgaaatt gcatccatgg
     1681 tgccggattg gctcgtggca atcgccatac ggcaggaact ggaaatgcac tttcgtatcg
     1741 tcagacagaa ggcgttagag cactcaaagc gataactgat atggctcagg tttcgggaga
     1801 gaagcatccg agcgcatcga tactggcggg tctctatgtc attctggatc cgtcggtttc
     1861 ttccgatcgt ccgcttgtcg atgttctgaa catatccgcg acagccggcg ccaaaatatt
     1921 tcagtaccga aacaagacgg cttcgatgaa agcagcttat gcggaggcgt tgccgctgag
     1981 gaaagcggcg catgagctcg gcgtcctgtt gatcgtcaat gaccgatgcg atttggcgct
     2041 ggccgtggat gcggacggtg tgcatctcgg acagggagac ttgccgctca atctcgcacg
     2101 aaaggtcatg gggcctgaca agctgatcgg tatttcgaca cacagccgag agcaggtgtt
     2161 ggcggcaacg gccggcgggc caaactatct cggatttggt ccgattttca tgcccggctc
     2221 aaagttagac catgatctgg tcgttggtct gcaaggac
//
LOCUS       DATNDD010000140         4627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2424923, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000140 DATNDD010000000
VERSION     DATNDD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424923"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VN647_03355"
     CDS             complement(<1..1330)
                     /locus_tag="VN647_03355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWG96100.1"
                     /translation="MTDFSRYIVNPGPKAAVSGLKAAVTTDSAIVTETMLRVLSGGGN
                     AVDASIAGALVQAAVEPFMTNHAGLVTALYYEAKTRQIHQLDSLGAFPSGLPPFKPVP
                     QGTGPYAVMPPSGIIPGFMPGLKEFHRRFGICRWSELCVDAVLWAERGHPVSTFEYGV
                     NVHNEKFITYFPEGREFYQSNGFFTNVGEMFVPSGLATTLRGVAEQGPDYMIVGPWAH
                     DFIAKANEMGWKIRLEHMTETPPRWLEPIRYRHHEFEVVSLGPPQAQGLYIGVALGVL
                     KHLGIRSMKVGSAEHFWAMGHALRQAARHWEYAQDEAIYGVPRDEVLDDHYHEHLAKM
                     IRKSRPKVDLTEHIRLSGDVAKAGDAMAALSGAGSKPRLSCAEAAQPSGSCEISVVDA
                     EGNWVQMMNTLQGSGIPGMVVGGVPMVGSHSTFGGLMGSMDATLVKGAKPR"
     gene            complement(1407..3776)
                     /locus_tag="VN647_03360"
     CDS             complement(1407..3776)
                     /locus_tag="VN647_03360"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HWG96101.1"
                     /translation="MNRRASRRGIIRSGRRRTLLGYAWPLTVRPGESVEFKVCSCPTS
                     RYRADLVRIICADDLAGALDFKEREVGAPFAGHYVGRFQETHTGSYVEIDSRGILAGL
                     DGFTVQTCVFPTALPKTKVRKLPSPVAAPQAAQHLVSCWDQLTQSGWALLIDDDNCPA
                     FQIGQCEGLSRVSLPSALKEGHWFLIAASVDLRRGQLRISAEPLNKAPAELLTWSGRD
                     AQAELSVETHSLPRLPIRFGAGANGAGNGERRKPIFCFNGRLDRVRLTKDTLTQLEVR
                     ALKGSEIPEALRPRILGFWDFSKGIDTVCVYDQTSNSVHGTAINLPTRGVAGIDWDGS
                     CRDWRAQPAHYSAIHFHDDDLYDAEWQTDFVYSVPEDLPSGIYSARLRAGDSEDYIPF
                     FVAPRRGTHTAPIALLLPTATYTAYTNLCWLNRKLKRRVKQSSGDSINVEQEAYPWVL
                     RTEEDEVFIANGLRERSLAKGVYLHHTDGSYCDTASQRVPNMTLKPKGRNWGLVADTY
                     ITDWLEHKGFSFDVITDDILHAEGLALLMQYSVVITGNHPEYCSRQMLDAIRQYQSEG
                     GRWMYLGGNGFYWVTSYHSQLPGVLEVRKEASYPGHHPPYELCHAFDGEGGGLWSSQG
                     RAQHALVGVGSVVPLPFEGSVPYERLPGSYHPRAGFIFDGVNAKLLGDFGIIGGGAAG
                     QETDAVDYDGGTPSHALHLARSREFPWPLLGPDGLTAEEYRDNVCMPRADMVFFEGPR
                     GGAVFSVGSMAWVGALSHESYENDISRITENVLRRFLDNDPFPIPDTDT"
     gene            complement(3773..>4627)
                     /locus_tag="VN647_03365"
     CDS             complement(3773..>4627)
                     /locus_tag="VN647_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HWG96102.1"
                     /translation="ILCAGAVNSPKLLLLSGIGDREELERHGIRVNVELPSVGCFFKD
                     QPFLRMTYRSKVPTYNLTGGLRQKLAIAAKFVFHGEGPISNLFEAVGFLKSRPSEVMP
                     DIRLTFLALGFVESSEGHSKLAPFPALMVSLMKSYPVSCGRIRLVSNDPAERPRIEYP
                     LFANQADVDTMIRGIHTIREIMTAEPIAGLIEEEIAPGQGVQSEAALERFVRKHATVA
                     LHSIGTCRMGVTDDAVVSPELRVHGLENLWVADASIMPDHISADTNATSMMIGAKLGR
                     QLCRGSSR"
BASE COUNT         1062 a         1386 c         1198 g          981 t
ORIGIN      
        1 aacgcggctt ggcgcccttt actaaggtcg cgtccatgga ccccatcagc cctccgaagg
       61 ttgagtggct ccccaccatc ggcacacctc caaccaccat tcctgggata ccagaacctt
      121 gcagggtatt catcatctgt acccagtttc cttctgcatc caccacggat atctcacagg
      181 aaccggatgg ttgagcggcc tctgcgcaac tcaggcgcgg tttgctgccc gccccagaaa
      241 gtgcggccat ggcgtcgccg gcctttgcaa catcaccact gagacggatg tgctcggtca
      301 agtccacctt agggcgtgac ttgcgaatca tcttcgcaag atgctcatgg tagtgatcgt
      361 cgagaacttc atcccggggc acaccataga tcgcctcatc ttgcgcatat tcccagtgcc
      421 gcgccgcctg tcgcagagca tgccccatcg cccagaaatg ttccgccgac cccactttca
      481 tgctccggat tccgaggtgc ttgaggactc cgagcgcaac accaatgtag aggccctggg
      541 cctgcggagg accgaggctc acaacttcga actcgtgatg tcggtagcga atcggttcga
      601 gccaacgcgg tggtgtctcc gtcatatgct caaggcgaat tttccatccc atttcgttcg
      661 ccttcgcgat aaagtcatgt gcccaaggtc caactatcat gtaatcaggc ccctgttcag
      721 ccacgcctcg cagagtggtt gccagacctg acggaacaaa catttcgcca acgttcgtga
      781 agaatccgtt cgactgatag aactcgcgac cctccggaaa ataggtaata aatttctcat
      841 tgtgcacgtt cacgccgtat tcgaacgtcg agacaggatg cccgcgttcg gcccacaaca
      901 ccgcgtcgac acatagctca gaccacctac agataccgaa gcggcggtga aattccttta
      961 gcccaggcat gaatcctggg ataatgcccg acggaggcat taccgcatac gggccagtgc
     1021 cttgcgggac gggcttaaat ggaggtaggc cagagggaaa cgcgccaagg ctgtcgagct
     1081 ggtgtatttg ccgcgttttc gcctcatagt agagagctgt cacaagaccg gcgtgattgg
     1141 tcatgaacgg ctccacagcc gcttgcacca acgcaccggc gatgcttgcg tcgacagcgt
     1201 taccgccgcc ggacaggact ctgagcatgg tctcggtgac gattgcggaa tcggtcgtca
     1261 cagcagcctt caaacccgaa accgctgctt tggggccggg gttaacgata taccgcgaga
     1321 aatcggtcat tttctatctc aacgtggagt cactgctcgc gaaaagagga ggtcgatttc
     1381 caggcgctta gaaggcggtg gagaactcac gtatctgtat ccggtatcgg aaacgggtcg
     1441 ttatcgagaa agcgacgtaa cacgttttcg gttatacggg agatgtcgtt ttcataactc
     1501 tcatgactca atgcacccac ccacgccatc gatcctacgc tgaaaacggc tccgccgcgc
     1561 ggaccttcaa agaacaccat gtccgcccga ggcatacaaa cgttatcccg atattcttcg
     1621 gctgtcagac cgtcagggcc cagcaaaggc cacgggaact ctcgactgcg agccaagtgt
     1681 aaagcgtgtg acggggttcc cccgtcatag tcgaccgcat cggtttcctg acctgctgcg
     1741 ccaccgccga taatgccaaa gtcacccaat agtttggcat tcaccccgtc aaagatgaaa
     1801 ccggccctgg ggtgatatga ccccggcagc cgctcgtaag gcacgctgcc ttcgaatgga
     1861 agaggtacga cggaacccac tccgacaagg gcgtgttgcg cgcggccctg actgctccag
     1921 aggccgcctc cttctccgtc aaaagcatgg caaagctcat atggcggatg atgacccgga
     1981 tatgaggcct cctttcgcac ctccagtacg ccaggcaact gcgagtgata gctcgttacc
     2041 caatagaatc cattaccacc gagatacatc caacgaccac cctccgattg atattggcga
     2101 atcgcatcga gcatctgccg tgagcagtat tctgggtgat ttccggtgat aacaaccgag
     2161 tactgcataa gtaaagccaa tccttcggca tgcaaaatat cgtccgtaat gacgtcaaag
     2221 gaaaatccct tatgctcaag ccagtctgta atgtacgtgt cagccaccaa accccaattc
     2281 cttccctttg gcttcaaggt catgtttggg acccgttgcg acgcagtatc gcaataactt
     2341 ccatctgtat gatgtaggta tacaccctta gccaagctac gctctcgcaa cccgttcgct
     2401 atgaacacct cgtcctcttc cgtccgcaag acccagggat atgcttcctg ttcgacattt
     2461 atcgagtcac ctgaagactg ctttacgcgc ctcttaagtt ttcgatttaa ccaacataaa
     2521 ttggtgtatg cggtatacgt ggcagtcggc agcaacaacg cgattggcgc ggtatgcgtc
     2581 ccgcgccggg gggcaacgaa gaaaggaata tagtcttcgg agtcacccgc cctcagtctt
     2641 gcagagtaga tgccgctcgg aaggtcttcc ggtacgctgt acacgaagtc cgtttgccac
     2701 tcggcatcgt aaaggtcatc gtcatgaaag tggatcgcgc tatagtgagc aggctgcgct
     2761 cgccaatctc ggcacgaccc gtcccagtcg atacccgcta cgccgcgagt tggcagattg
     2821 attgcagtgc cgtgcacgct gttacttgtc tgatcgtaaa cgcaaactgt atcgatccct
     2881 ttgctgaaat cccagaaccc gagtatccgt ggtcgcaacg cttcggggat ctcggacccc
     2941 tttaaagctc tcacctcaag ttgagttagc gtgtccttag ttagcctcac tcggtcaagc
     3001 cgaccgttaa agcagaagat gggtttacgc cgctctccat ttcccgctcc gttcgcgccg
     3061 gcgccaaatc ggatgggcaa tctcgggaga gagtgagtct caacggataa ttcggcctgc
     3121 gcatctcgtc ctgaccacgt cagcaactcc gcaggggctt tattaagcgg ctccgcggag
     3181 attcggagct gacctcgacg cagatcgaca ctggcagcta ttaagaacca gtgtccctcc
     3241 ttcaaagcgc tcggcaatga cactcgcgac aagccctcac attgcccaat ctgaaacgcg
     3301 ggacaattgt cgtcatcgat cagcaacgcc cacccgcttt gggtcaactg atcccaacac
     3361 gaaacgagat gctgagctgc ctgcggagcg gccaccggtg aaggcaactt tctgaccttt
     3421 gtcttaggta aggcggtcgg gaatacacat gtttggaccg tgaagccatc cagacccgcg
     3481 agaattcctc gcgagtcgat ttctacgtaa gaccccgtat gagtttcctg aaagcgcccg
     3541 acatagtgac ccgcgaaagg cgcacctacc tccctttcct tgaaatccaa tgcccccgcc
     3601 aggtcgtctg cacagatgat ccgaaccaag tcagctcggt agcgcgacgt ggggcaagaa
     3661 caaactttga attccaccga ttcgcccggt cggactgtca aaggccatgc atatcccagc
     3721 aaggtacgcc gacgacctga gcgaatgatg ccccggcgtg atgcgcgtcg gttcatcgcg
     3781 aactccctcg gcacagttgc ctaccgagct tagcgccgat catcatgctt gtggcgttag
     3841 tatctgcgct gatatgatcg ggcatgattg aggcgtcggc cacccaaagg ttttccaacc
     3901 catgcacccg aagttctgga ctgacgaccg cgtcgtccgt gacgcccatc ctacacgtac
     3961 cgatcgaatg gagggccacc gtggcgtgct tacgcacgaa gcgctcgagc gccgcctcac
     4021 tctggacgcc ttgaccggga gcgatctcct cctcgataag accggcaatg ggttcggcag
     4081 tcataatctc ccggatcgtg tgaatacccc gaatcatcgt gtcgacatcg gcctgattgg
     4141 cgaacagcgg atactcgatt cgcggtcgct cggcagggtc gttgctgacc aagcgtatgc
     4201 gtccacagct tacaggatag gatttcatga gcgataccat gagagcagga aacggcgcca
     4261 acttagaatg cccctccgaa ctctcaacga aacccaaggc taaaaaggtc agtcggatgt
     4321 caggcatgac ttccgatggc ctggatttga gaaaaccgac cgcctcgaag agattcgaga
     4381 tgggtccctc gccgtggaac acgaacttcg ccgcgattgc taacttttgg cgcaatcctc
     4441 ccgtcaagtt gtacgttgga actttggagc ggtaggtcat ccgtaagaat ggctgatcct
     4501 tgaagaaaca cccaacggaa ggaagctcta cattgacgcg gataccgtgg cgctctaatt
     4561 cttcccgatc cccgatacca gaaagcaaca gcaacttggg ggaattgact gcgcccgcgc
     4621 acagaat
//
LOCUS       DATNDD010000141         2968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_221305, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000141 DATNDD010000000
VERSION     DATNDD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221305"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VN647_03370"
     CDS             complement(<1..253)
                     /locus_tag="VN647_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96103.1"
                     /translation="MRSSNLGAGKAGVAGLVLGSLILAGLGLENASAETPTPPTDFQS
                     LTQSWDKKLPGASRFTVLSDFGGAAVRDNETGLVWEQSPE"
     gene            complement(280..813)
                     /locus_tag="VN647_03375"
     CDS             complement(280..813)
                     /locus_tag="VN647_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG96104.1"
                     /translation="MVQQQGPVLLVGHSYGGAVITEMGDLPNVGGLVYIAAFSPDVGE
                     SPGGISQSKPPAAIANLAPDSDGYLWILPDKFHESFCQDLTPDEALVMAVTQKAPLAS
                     TFGDRITAPAWKRKPCWYQVSREDRMIHPENQTHMAGRMNPRKIVTLAASHASLASKP
                     VEVSGLIDEAATALAKV"
     gene            complement(813..992)
                     /locus_tag="VN647_03380"
     CDS             complement(813..992)
                     /locus_tag="VN647_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96105.1"
                     /translation="MTTKPNILFVHGFWGGAAHWSKVIIELSRKGYDKLRAVELPLTS
                     LADDAERTRKNAHVR"
     gene            complement(1142..1333)
                     /locus_tag="VN647_03385"
                     /pseudo
     CDS             complement(1142..1333)
                     /locus_tag="VN647_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            1824..2705
                     /gene="hchA"
                     /locus_tag="VN647_03390"
     CDS             1824..2705
                     /gene="hchA"
                     /locus_tag="VN647_03390"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: protein motif:HMM:NF003168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HWG96106.1"
                     /translation="MTGKHTEADKHPTPDPAENNAFFPSAYSLEKFTSSKSDLEGADY
                     PNLYREGKWKVLMIASEERYLLMQNGTMFSTGNHPVEMLLPMYHMNKAGFEFDIATLS
                     GNPVKLELWALPKEDEAVQSVYQKYLRKLKSPLKLSDVIESKLGDDSNYIAVFIPGGH
                     GALIGIPDSKDVKHVLEWAMRKDKHVISLCHGPAALLSAALGESKDDFMYKGYKICVF
                     PDSIDKKNPDIGYMPGPMPWFVAEKLKRLGVEILNSDITGKTHQDRKLITGDSPFASN
                     NVGKASAQALLKEVGLS"
BASE COUNT          708 a          811 c          761 g          688 t
ORIGIN      
        1 tctccggcga ttgttcccag accaaccctg tctcattgtc ccgcactgct gctccgccga
       61 aatcactcaa gacggtaaac cgagaggcac cgggtaactt cttgtcccag ctttgggtca
      121 ggctctgaaa atcagtcggt ggagtcggtg tctcagccga cgcattttct aagcccaaac
      181 ccgcgagaat caggctcccg agcactaacc ctgcgactcc ggctttacct gctcctaggt
      241 tgctgcttcg catgatgctc cctcctccgt gtgttgggat tagactttgg caagtgcggt
      301 cgccgcttcg tcgatcagtc cagacacctc gaccggtttc gacgccagtg aggcgtggct
      361 cgccgccaag gtcacgatct tgcgcggatt catccgccct gccatgtgcg tctgattctc
      421 cggatgaatc atccggtcct ccctggaaac ctgataccag cacggcttcc ttttccatgc
      481 cggggcagtg atcctgtcgc cgaaggtgct ggcaagcgga gctttctgcg tcacggccat
      541 caccaaggct tcgtccggag tcagatcttg gcagaaactc tcatggaact tgtcgggcag
      601 aatccagagg tagccatcgc tgtcgggcgc aagattggcg attgccgctg gcggcttgct
      661 ttggctgatc ccaccgggac tttccccgac atcgggcgag aaggcagcga tgtagactag
      721 gccgcccacg ttcggaagat cgcccatctc ggtgatcacc gcgccgccgt atgaatgtcc
      781 aaccaacagc acggggccct gctgttgcac cactatctta cgtgtgcgtt cttacgtgtg
      841 cgttcggcgt cgtcggccag cgatgtcaac ggcaactcga ccgcgcgcag cttgtcatat
      901 cctttgcgcg acagttcgat gatgaccttg ctccaatgcg ctgcgccgcc ccagaaccca
      961 tgcacgaata ggatgttggg tttcgtcgtc atggtaatcc tcctacttgt aggaagttcc
     1021 ctcacaagcc cgaatcatgc acctcaattt ttggagatct gtgggtaatc gtctgtcatt
     1081 ctgaagtcac gtgaggagcc gttcatggat attagatcgc cgagtctcct aaacgactct
     1141 tttactccac tatagcccgt agcatccagg ccatcttttc atgtcgctcc atgagtccgg
     1201 tgagaaaatc gttcgtgccg gagtcgtgaa acttatcgcc gcaactttca agatccactc
     1261 gcaacggctg aataatctct tcatgactca ttaataactt tttgatcatc tcaatggcgc
     1321 tcaaagattt ctcagctgtt ccttcagtct cgtgtgtttc agaaactcag tgaggatggg
     1381 aggccagtgt acgagggtag cgaactaaga cgcaagtcta ggcccaccgt ggagaaccgt
     1441 caggccgtaa cgaagtgccg gagctttatg cttgaaggta acaaggcgaa ccgatgcggg
     1501 gtcatgaact gatagacgag aggggagcaa ccggagagaa caaatcaggc catgcaaagc
     1561 tgcatagcct acaagaaaat cagctatgaa aaccgacaaa cttttcccct ttccccaatc
     1621 ggacagcttt gctactgtga agtttcctct ctgaacatgt gattaggatt ctaccgagtg
     1681 gtggtctcaa tcgatgtcac actcgcatgg caaactaact tgcagtgccc caagcaaggc
     1741 aacgccggcg cacttaataa ttcaaaaata gaaagggaaa ccatgaaatc tccattcacg
     1801 aatcaagcga gaggaggaaa gccatgaccg gtaagcatac cgaagccgac aagcatccta
     1861 cccctgatcc ggctgaaaat aatgccttct ttccatcggc ctactccctg gaaaagttta
     1921 cgtcgtccaa atccgatttg gaaggggcgg actacccaaa cctctatcgc gaggggaaat
     1981 ggaaagtgct gatgatcgcc tctgaagaga gatatctcct catgcagaac ggcacgatgt
     2041 tctccacggg aaaccacccc gttgaaatgc tgcttccgat gtaccatatg aacaaggccg
     2101 gcttcgaatt cgacatcgcg actctgtcgg gcaaccctgt caagttggaa ctttgggcat
     2161 tgccgaaaga agacgaagcc gtccagagtg tgtatcagaa gtacctgaga aaacttaaaa
     2221 gcccgctcaa gctatcggat gtgatcgaga gcaaacttgg agacgactca aattatatcg
     2281 ccgtcttcat cccggggggg catggtgcct tgatcggaat ccctgacagc aaggacgtca
     2341 agcacgttct cgaatgggcg atgaggaaag acaagcatgt tatttccctc tgtcatgggc
     2401 cggcggcgtt gctatcggcg gcgctcgggg agtccaagga cgacttcatg tataagggct
     2461 ataagatctg tgtcttcccc gattccatcg acaagaaaaa tcccgatatc ggatacatgc
     2521 ctggcccgat gccatggttt gtcgccgaaa agctgaagcg gctcggtgtt gagatcctca
     2581 atagcgacat caccggaaaa acccaccaag atagaaagct cattaccggt gatagtccgt
     2641 ttgcgtctaa caatgtcggt aaagcaagtg ctcaggctct cttgaaggag gttgggttga
     2701 gctagagcat gcaatgtcca gagaggagaa ccgcgatgat gaccaaactc cacatcgtct
     2761 tgatccttct tctataagcc tctacctata ggtctctgcc gagaggactt catttaggag
     2821 ttggaataga cacatcgtaa gcccttcgca cttgggtgtc ttgggctaat aaggccttga
     2881 catctcatgc ttgctgagca gattctcact gcgtgtttta tgctatgaaa aacgtcttgc
     2941 gggccgaccc gacgccgatg agacttgc
//
LOCUS       DATNDD010000142         3382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1321934, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000142 DATNDD010000000
VERSION     DATNDD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321934"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..303)
                     /locus_tag="VN647_03395"
     CDS             complement(73..303)
                     /locus_tag="VN647_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96107.1"
                     /translation="MLCIRCGGLTVLDEWEDLFEVRESISIEIWRCICCGAREYPLRL
                     ENSQLRECRSGTPYDGRTHDLSRPAIKQDLQY"
     gene            complement(407..754)
                     /locus_tag="VN647_03400"
     CDS             complement(407..754)
                     /locus_tag="VN647_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96108.1"
                     /translation="MNVRRFAIPIAAWALISISTANGAETSVIKIVSPTDRAQINAEQ
                     EYPLTYEVALGPGGNHFHVWVDDKRGPGVRDLNGTYLLPGLSPGEHVITLRIVDRQHS
                     PTGVEKSIRLTAK"
     gene            975..1730
                     /locus_tag="VN647_03405"
     CDS             975..1730
                     /locus_tag="VN647_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96109.1"
                     /translation="MTSQAIADRRNEWFVPSRGPLNVRVLIGLLFLPYTGMVLSYTII
                     GAMLAERIYWDRVGAIVTIYFLALGIGAHALDAVGSKNVKPWGTVFSDGPLWAVALSS
                     ITIAYTIGIYYMVLYTPLLALIALVEGFFLFAYNLEWFGGRFHTDPWFAVSWGGLPVL
                     AGYILQTNRLSLMALVVAISTGLFSLVEIKTSRPYKALRCRFPTTKTEGDEEQREMLQ
                     RYEVILKSLSAAVLLLGIGLLMWRITSPVCSPS"
     gene            complement(1702..2406)
                     /locus_tag="VN647_03410"
     CDS             complement(1702..2406)
                     /locus_tag="VN647_03410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96110.1"
                     /translation="MQTHDARVELVHRFFSGTGRTYDAVVHYATFGIDRRWKRRIIEL
                     LPPNPRRILDLACGTGISTLAIAEQFPHCRVIGVELREEYLEIARSKIHKLGLVNVDF
                     VLSRAEDYRSAEPFDCVVSSYLAKYADLERLIPVTKTMLRKGGLLLMHDFTYPTKPCL
                     IPMWRLYFWMLQRVGARLCPAWREIYYGLPGLIQNTRWIQELAEVLKEQGFENIRLEY
                     LTAYGAAIMTASTQDS"
     gene            2597..3157
                     /locus_tag="VN647_03415"
     CDS             2597..3157
                     /locus_tag="VN647_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWG96111.1"
                     /translation="MGLIRNLQPERSSEQDEPSRFEEAILPHLDAAYNLARWLTRNDQ
                     DAEDMVQEACLRAFKSFDGFYGVKSKAWLLTIVRNTCYTWLGKNRVQDLSTSLDEDVH
                     QAESQVPDPQTLLLRFEDNQALRQALEELPAEFREVLILRELEELSYKEIADVIGIPP
                     GTVMSRLARARGRLKLSLADRMNKEH"
     gene            3159..>3382
                     /locus_tag="VN647_03420"
     CDS             3159..>3382
                     /locus_tag="VN647_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWG96112.1"
                     /translation="MNCQEARTLIDGYMDGELDPIRNREVEQHFRTCPVCLTVYNSHR
                     ALRTALRTGSLYRTAPPALRNRVQSSFKPAP"
BASE COUNT          697 a          939 c          960 g          786 t
ORIGIN      
        1 atgcgcaggt ttgccgtatt ctcatgagat cctccttcga ggtaaggtta taggtaaact
       61 accgtttccc cctcagtact gtaggtcttg cttgattgcc ggtcgcgaca ggtcatgcgt
      121 gcgaccatca tatggcgtac cgctgcggca ttctcgaagt tggctgttct ccagccgcag
      181 cggatactca cgagccccgc agcagatgca acgccatatt tcgatcgaga ttgactctct
      241 gacttcgaac agatcttccc actcgtccag gaccgtgagt cctccgcatc ggatgcacag
      301 catgtgtcct ccttgcatgt tgaaggtcat gagacggcgg tcgattcggc cgtcgatttc
      361 cgtgtgatcg ccccgtccga gccgcctgcg tgtttctgct tccttgtcac ttcgctgtga
      421 gccgaataga cttctcgacg cctgtcggac tgtgctgcct gtccacgata cggagggtga
      481 tcacatgttc gcctggactc aagccgggta gaaggtacgt gccgttcaag tcacgtacac
      541 ccggcccgcg tttgtcatcc acccagacgt ggaaatgatt cccgcctggc cccagtgcga
      601 cctcgtaggt taacgggtat tcttgctcgg cattgatctg cgcgcggtcg gtcggggata
      661 caattttgat aaccgatgtc tccgctccat tggcagttga aattgagatc agggcccacg
      721 ccgcaatcgg aatagcgaaa cgcctcacgt tcatgacaga cctccttggt tgagaattgg
      781 gtgcgatgtc cgttcactgt gtgtaaccgc cgcatcgtga aaatattccc gccatgcaga
      841 agtttttggt gggaatagaa tcgcccatcc gtcggtctca agagaatcaa agcaacaagc
      901 ctgcgacagt catcacggcc gatggaacgc gtcgccggtg aatgaggtgc tctcatcgac
      961 acagggagat gaccatgacg tctcaagcga tcgccgatcg aaggaatgaa tggtttgtgc
     1021 cttcccgtgg cccgctcaac gttcgggtgc tgatcggcct gctctttctt ccctatacgg
     1081 ggatggtgct gtcctacacg atcatcggcg ccatgctggc tgagcgtatt tactgggatc
     1141 gggtgggggc gatagtgacg atttatttct tggccctcgg tatcggggcg cacgcgctgg
     1201 atgccgtcgg cagcaagaac gtcaagccat gggggaccgt gttttctgat gggccactct
     1261 gggccgtagc cctttcttcg atcacgatcg cttacaccat cgggatctat tacatggtgt
     1321 tgtacacgcc tctgctcgcg ctgattgcct tggttgaagg gttctttctg ttcgcctaca
     1381 atctggagtg gttcggcggc cgattccaca ctgatccttg gtttgccgtc tcgtggggag
     1441 gcctgcctgt tctcgccggg tacattcttc agacgaatcg gctttcactg atggccctgg
     1501 tcgtggcaat ctcgacgggc ttgtttagtc tcgtggagat caagacgtca cggccgtata
     1561 aggcgttgag gtgccgtttt ccgacaacaa agacggaagg cgatgaggag cagagagaga
     1621 tgcttcagcg gtacgaggtg atcctcaaaa gcctcagtgc ggcggtcctc cttctgggaa
     1681 tcggactgtt gatgtggcgt atcacgagtc ctgtgtgctc gccgtcatga tggcggctcc
     1741 ataggcggtc aggtattcca gccggatgtt ctcgaagccc tgttccttga gcacctcggc
     1801 caattcctga atccagcgcg tgttctggat cagaccgggc aggccgtagt aaatttcccg
     1861 ccatgcggga cagagccgag cgcccactcg ctgcaacatc cagaaataca ggcgccacat
     1921 cggaatcaag cagggcttgg tcggataggt aaagtcatgc atgagcagaa gaccgccctt
     1981 ccttagcatg gtcttggtga cggggatgag gcgttctaag tcggcatatt tcgccagata
     2041 ggaagagacc acgcagtcga acggttcggc ggatcggtag tcttcagccc ggctcaatac
     2101 gaagtccaca ttaaccaagc caagcttgtg gattttgctt cgagcaatct cgagatattc
     2161 ctcccgcagt tcaacgccga tcaccctgca atgcggaaac tgttcggcga tcgccagcgt
     2221 agaaataccg gtgccacagg cgaggtccag aatacgtcgc gggttcggag gcaacagctc
     2281 gatgattctc cgcttccacc ggcggtcgat accgaaggtt gcgtaatgaa ccactgcatc
     2341 gtaggtcctc cctgtaccgg agaagaaccg atggaccaat tccactcgtg catcgtgcgt
     2401 ttgcatgaca aaccctcctt tccatcgcca tctcctattc tgaccttcct cgcatggttt
     2461 ctattcccga cgttgcgttg atttgatggc cgcagtgctg ttaagtaggt gccgtgtcgc
     2521 cgggattgag cgatatagca acgcgactcg ctaggatcac atcacccaat tgcatcggga
     2581 ataaatcggc tcagaaatgg gtcttatcag gaacctgcaa ccggagcgat cgtctgagca
     2641 agacgagccg tcacggtttg aagaagccat cctcccgcat ctcgatgcgg cttacaattt
     2701 ggcgcgctgg ctgacacgca acgaccaaga tgcggaagac atggttcagg aagcctgctt
     2761 gcgcgcgttc aaatcgttcg acggcttcta tggagtcaaa tcgaaagcgt ggctgctcac
     2821 gatcgtgcgg aacacctgtt acacgtggct cggcaagaac cgagtgcagg acctgtccac
     2881 ctcgctcgat gaagacgtgc accaagcgga aagtcaggtt ccggacccgc agacgctgct
     2941 gctccgattt gaagataatc aggcgcttcg gcaagccttg gaagaattac cggcggagtt
     3001 ccgcgaagtg ttgatcttac gagaattgga agagctttcg tataaggaga tcgccgatgt
     3061 catcggcatt ccaccgggga cggtcatgtc tcgtctggcc cgcgcgcgag gtcggttgaa
     3121 actctcgctt gccgatcgca tgaacaagga acattgacgt gaattgccaa gaagcccgga
     3181 cgctcattga cggctatatg gatggagagt tggatccgat ccgcaaccgg gaggtcgaac
     3241 agcacttccg gacttgtccg gtctgcttga ctgtgtacaa cagtcatcgg gcgttgcgaa
     3301 cggcattacg aaccggttcg ctctaccgga cggcgccccc ggccctgcgg aaccgagttc
     3361 aatcatcctt caagccggcg cc
//
LOCUS       DATNDD010000143         4208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2762396, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000143 DATNDD010000000
VERSION     DATNDD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762396"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VN647_03425"
     CDS             <1..727
                     /locus_tag="VN647_03425"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HWG96113.1"
                     /translation="NSATNKLPCSRRFRRKKHRKGMTDMKRRVLRYAAIVLSLPMGIV
                     MWQPSVFAQGQPLVLEDFQAKEADGFPSQWDHENQRSQSKGREAYKVRSETGLSFLSA
                     KDAGQRIKKKKIDWDPKAYPILTWRWRLNKATTGTEPLAAVYASLDTDLLFIPVFTKY
                     VWSGSKPDGTLTEGGMFSGSEIVVQTGIKEVGQWFEERVNVYEDFKRIHQHEPAEKAW
                     GISIIAAPGVEIDFGPLIATPIK"
     gene            766..1131
                     /locus_tag="VN647_03430"
     CDS             766..1131
                     /locus_tag="VN647_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96114.1"
                     /translation="MVAREKVFDIALGITVMGTVGALIGLTMGGGLMPIAIVVGIVLG
                     IVIGFLGGRRFLISILIGTVLGGALAWMLAGAERIWVGAGAGAAMGGFLGVQISMLLD
                     VRAAKKAASEQGETSSSYR"
     gene            1156..2073
                     /locus_tag="VN647_03435"
     CDS             1156..2073
                     /locus_tag="VN647_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWG96115.1"
                     /translation="MENRGVLVGSIVFVFTSFFLMIGLLAYESYKAKQMKQLAASVKS
                     EARPTTSKLPTQDFSMYKTKVGDEGREMVQIPEGPFTMGSNEGDPDEAPEHQVYLKGF
                     YLDQKEVTQEEYMRFAKMTKRAMPRIEVFEDDQSKILKSELAAMSVSWDEAAAYCKWA
                     GKRLPTEAEWEKAGRGESKKRYPWGDKFVANAANVDGSEDGYKYLAPPGSFDAGRSPY
                     GLYDMTGNVAEWVEDSYGEHYYKKSPFRDPKGPENQDLKVVRGGSWKETEHNARLSKR
                     FAAKHWRTDVTIGIRCASDLDQVTGSPES"
     gene            2080..2979
                     /locus_tag="VN647_03440"
     CDS             2080..2979
                     /locus_tag="VN647_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWG96116.1"
                     /translation="MLETKFKVVFLLVVLVCAAMPITAILRGTTLTPYEEQESGTSSE
                     TEPERDSASGGDPVPEDIVTIPAGPFVRGTEDGGFDEQPQRTIFLDAYSIDRYEVTNH
                     QYQQFVASTGHRKPGLPSRYAKSGSKVRGINQPVVYVSWDDASEYCRWKGKRLPTEAE
                     WEKAMRGTDGRLWPWGNKEQANGANWARVQDGHEVSASVGSFQADKSPYGVMDGAGNV
                     IEWVNDWYDETYYKSSPEQNPPSPEYGTYRVLRGGGYTTTGGDVRITSRSKMMPDFRD
                     ETIGFRCAVSKTGMSEEAKKKAG"
     gene            3620..>4208
                     /locus_tag="VN647_03445"
     CDS             3620..>4208
                     /locus_tag="VN647_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96117.1"
                     /translation="MGLLTGKRVFSIMALCMMVGLLLLPIVIALPSPAIGEDAPAGDA
                     AKKDGDKVEKGRDVYYKTEGIVVGAHAPKTTDGPKDYPRYNFESRVLIWFANQQHLYY
                     GSFVLAVPIFCMIIEFMGVVTKDKALAKRYDQLAYDFIKISLTAYSLTAILGGILIFT
                     FLTLYPAFFGYLSSIFRPVMHIYALMFVAESGTLYI"
BASE COUNT         1022 a          938 c         1236 g         1012 t
ORIGIN      
        1 gaattccgcg acaaacaagc tcccttgctc acgccggttc cgtcgcaaga aacatagaaa
       61 gggaatgacg gacatgaagc gtcgggttct gcgttatgca gcaatcgttc tgagcctgcc
      121 tatgggcatt gttatgtggc aaccatccgt gtttgcccaa gggcaaccat tggtcctgga
      181 agactttcaa gcgaaggaag cggatggatt tccttcgcag tgggatcacg aaaatcagcg
      241 aagtcaatcg aaggggcggg aagcctataa ggtgcggtcc gagaccgggc tgagtttttt
      301 gtcagcaaag gatgcgggcc agcggatcaa gaagaaaaaa atcgactggg atcccaaggc
      361 ctatcccatt ttgacctggc gctggcgtct caacaaggca acgaccggga ccgagccgct
      421 tgccgctgtc tacgcttcgc tggatactga tctcctcttt atcccggtgt ttacgaaata
      481 tgtgtggagt gggtcgaagc cagacggcac cttgacggaa ggcggcatgt tcagcggctc
      541 cgagatcgtc gtgcagactg ggataaagga ggtcgggcag tggtttgagg aacgggtgaa
      601 tgtgtacgag gacttcaagc gaattcatca gcatgagccg gcggagaagg catgggggat
      661 ctctattatc gcggcacctg gtgttgaaat cgattttggt ccgctgatcg cgactcccat
      721 caagtagcga ttgtcttttc ccgctctgtc tatgaaggac tgaatatggt ggcacgagaa
      781 aaggtatttg acattgcctt agggataacc gtgatgggta ctgtcggtgc tctaatcggg
      841 ctgactatgg gcggtgggct gatgccgatc gctatcgttg tagggattgt gcttggcatc
      901 gtcatcggct ttctcggcgg gcgtcgattt ctcatcagta tcttgatcgg gacggtgctg
      961 ggcggcgcgt tagcctggat gctggcgggt gctgagcgga tctgggtcgg cgcaggtgcg
     1021 ggggctgcca tgggtggatt tcttggcgtc cagatatcaa tgttgcttga tgtgcgtgct
     1081 gccaagaagg cggcatcaga acagggtgag acatcgtcgt cttatcggta attcctccac
     1141 gttgtagagg tgtccgtgga aaacaggggt gttttagtcg gatccatcgt ttttgtgttt
     1201 acctcgttct ttctcatgat cggtctgctg gcctatgagt cctataaggc aaagcaaatg
     1261 aaacagcttg ccgcgtcagt gaagtcggaa gctcgaccga cgacgagcaa gttgccgact
     1321 caggatttct cgatgtacaa gacgaaagtg ggggatgaag gtcgggagat ggtgcagata
     1381 cccgaaggcc cttttacgat ggggagcaat gaaggggatc cggatgaggc tcctgagcat
     1441 caagtttacc tcaagggatt ctatctcgac caaaaagaag tcacccaaga ggaatacatg
     1501 cgcttcgcga aaatgaccaa gcgcgctatg cccaggatcg aggtgttcga agacgatcag
     1561 tcgaagattt tgaagtcgga attggctgcc atgagcgttt cctgggacga ggcggccgcc
     1621 tactgcaagt gggccggcaa gagacttcca acggaagcag agtgggagaa ggccggccgt
     1681 ggcgagagca agaaaagata tccgtgggga gataagtttg tcgcgaatgc cgccaacgtc
     1741 gacggcagtg aagacgggta taagtatctt gccccccccg ggtctttcga tgcgggacga
     1801 agtccctacg gcctctatga catgacgggt aatgtcgcgg aatgggtgga ggattcgtac
     1861 ggcgagcatt actataagaa atctccgttt cgtgatccca aggggccgga gaaccaggat
     1921 cttaaggtcg tacgcggggg ctcatggaag gaaaccgaac acaatgcgag actgtcaaag
     1981 cgatttgcag ccaaacattg gcggacagat gtgacgatcg gcattcgttg cgcaagtgat
     2041 ctggatcaag tgacagggtc gcctgagtct tagtccgaaa tgctcgaaac taaatttaaa
     2101 gtggtatttc tgctcgtggt gctggtttgt gccgctatgc ccatcaccgc tatcttgcga
     2161 ggcaccaccc tcacgccgta tgaagagcag gagtcaggca ccagttctga aactgaaccg
     2221 gagcgagata gcgcttctgg aggggaccct gttccggagg acattgtgac gattccggca
     2281 ggcccattcg ttcgtggaac tgaggatggc ggattcgacg agcaaccgca gcgaaccatc
     2341 tttctcgatg cttactcaat cgatcgatat gaagtaacca accatcaata tcaacaattt
     2401 gttgcttcca caggccatcg aaaaccagga ctgccttcgc gctatgcaaa gagcggcagc
     2461 aaggtgcgtg gtataaacca accggtcgtc tatgtctctt gggatgacgc atcggaatac
     2521 tgccgctgga aaggcaagcg gcttccgact gaagccgagt gggagaaagc tatgcgcggt
     2581 acggacggga ggctctggcc atgggggaac aaagagcagg ccaatggcgc caactgggcc
     2641 agagtacagg acgggcatga ggtgtctgcg tcggtgggaa gctttcaggc tgacaaaagt
     2701 ccttatggag tgatggatgg agcgggtaac gtcatcgaat gggtgaacga ttggtacgat
     2761 gaaacatact acaagagttc gcctgaacag aaccccccga gccctgagta cggcacctat
     2821 cgggtacttc gcggcggagg ctatacgacg accgggggtg atgttcgaat tacgagtcga
     2881 agtaagatga tgccggactt tcgtgatgag acaatcggat ttcgctgtgc agtttcgaag
     2941 accggaatga gtgaagaagc gaagaaaaaa gcggggtgaa tttacagaaa atcaaagtag
     3001 tagagaatca aaaacacggc caaaatgata ttgacaacca ttccggccaa aactataatg
     3061 tcaaacactt ttgagttttt tgtcacggtt tctcctgttt gattcgctcg caaacgaaga
     3121 aatttgataa cacagggtgg aatgcctgtc aagaaaccgt agtgtttttg atgtctcata
     3181 attctatttt ctgaacagga gtaatgagca cgatggcaac agctacatct gacagtgaaa
     3241 ttaagataaa agtcggtaag atgattttct atatcacctg cgccgttggg ttatggtttt
     3301 tctactggtt tgctggagtc cagtgtccct gctgagatcg taggtctcgc ctaacctcag
     3361 ggtctggttc gcagttcaat caacagatgg ccaggtaagc agaggagaag agggaaatga
     3421 gcgcgttgaa taatccaatc gttgcagtga ttgtctctct tgttatcgcg gtcggctatt
     3481 ttacgtttgt tgatcattat ctaatggata tgcagggtct agactactgg tacctattcc
     3541 gaaagtaggc tgtgaggaag tagtcaagac ttctggtgaa cgtcctcaat ttaacgtaag
     3601 tgtattaagg aggtatccca tgggcctttt aaccggcaag cgcgtgtttt cgatcatggc
     3661 gctctgcatg atggtcggcc tccttctgct cccaatcgtt atagcgctgc cttcaccggc
     3721 tatcggtgaa gatgctccag ccggcgatgc ggcgaagaag gatggagata aagttgagaa
     3781 gggtcgggat gtctattaca aaacggaggg tattgtagtc ggcgctcacg ctcctaagac
     3841 cacggacggt cccaaggact atccgcgata taacttcgaa agtcgtgtct taatctggtt
     3901 tgccaaccaa cagcatcttt attatggaag ctttgtgttg gctgtgccga ttttctgtat
     3961 gatcatcgag ttcatgggcg ttgtgaccaa ggataaggcg cttgcgaagc gttacgatca
     4021 actggcctat gattttatca aaattagtct cacggcttac tcgcttacag ccattcttgg
     4081 tggcattctg atctttacat ttctgactct gtatccggcg tttttcggat atctgtccag
     4141 tatcttccgg ccggtcatgc atatctacgc attgatgttc gtggccgaga gcgggaccct
     4201 ctatatct
//
LOCUS       DATNDD010000144         4498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2728916, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000144 DATNDD010000000
VERSION     DATNDD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728916"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..890
                     /locus_tag="VN647_03450"
     CDS             294..890
                     /locus_tag="VN647_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96118.1"
                     /translation="MKGCGLPTSVEWVVFRLMPIVMAGSVQTATALDFSAERIVKNGE
                     SVVIAHVNAKEDRWRFEYAYPQRGVSIMIVRMDKQHSWLILSKRRQFLEVPIAADHVP
                     TVTERMEGEISREFVGDETLSGHPTELFEVTVVAQGETRQYYRWVTKAQRFPLKTVSK
                     QGKWSEEFRRVIFTEQSTFLFELPRRLDNANPSAGMQH"
     gene            912..1697
                     /locus_tag="VN647_03455"
     CDS             912..1697
                     /locus_tag="VN647_03455"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWG96119.1"
                     /translation="MRQPFPRGLWMAGCASVILILGFQADAGAYESGNVEDGAVVRGK
                     VTFTGTEPDPKEFELRRSPDREFCSAVSDGNGHRLLKEITIGPDGGLKDVVVVLEGVE
                     RGKPFTFTDAEVEASLCRFLPFVTVVSDKRRVTVFNRDPVLHDIQGYAYNEAGVDLVL
                     QRPALHESGTTDIVKLVKGRKVFTMQCGMHPYMQNWGYAIDNPYYAITDLNGSFAIGD
                     IPAGTYHLKAWHPILGTQERTITVKPNETISLDWLFDAKRVIE"
     gene            1738..2022
                     /locus_tag="VN647_03460"
     CDS             1738..2022
                     /locus_tag="VN647_03460"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWG96120.1"
                     /translation="MPTVGTDDVVAEVCRREKRVVVMLDKDFGDIRAYPPHEYAGLIV
                     LRVEDQQKKRLVALMNHVVNRLATEPIQGRLWIVEEQGIRVRMSWSEATE"
     gene            complement(2031..2660)
                     /locus_tag="VN647_03465"
     CDS             complement(2031..2660)
                     /locus_tag="VN647_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96121.1"
                     /translation="MKLLMIRGCLVFALVAMSLVPQATPALGAVTDTNEGTGWKLYTN
                     DRFGFSVRYPEDWRLGNPLPDGIGVTMYPPIEQSLVALSGHMNVVSGRSQDGRQTLKE
                     FADAHRRIITDLYDKKQVQVTWQKDQEATLAGFPAKRLAFTYQDDKRNAMLEVHILSL
                     GRNEGRGVRIKMPADRTDQLMPTITRMLKSYEAGRDQNAVSPIVPKGKG"
     gene            complement(2680..3213)
                     /locus_tag="VN647_03470"
     CDS             complement(2680..3213)
                     /locus_tag="VN647_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWG96122.1"
                     /translation="MAFTALLLKPLAGRAQDNSFYERIATLAHGSPMITVGQGHFVMG
                     TARTGQAARGEKGYLFPWGHKPPAIGLAMFGQYHLHEIPIVASVERGEEGRSPYGLHH
                     MAGNAAEWVEDWFGIDYYATMLDRNPCGAGQGRYKVVRGGSWKSAPPLLRTATRSGAA
                     PEQRTATVGFRCAKSIR"
     gene            complement(3379..3819)
                     /locus_tag="VN647_03475"
     CDS             complement(3379..3819)
                     /locus_tag="VN647_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96123.1"
                     /translation="MKRAMLVLVSLVLTSLIAFVTLMIRWMNFWIRGSYFVSYQTIFL
                     ITLTIFLLEWLRRHHTGPLPVSSTILYSIVVGYVSGLIAMALYPISQPDGLQQTALGL
                     RFPTPEAVAAFLWFPVRMLAWLFGGIAGLVILAMSRRFKPLLLE"
     gene            3879..>4498
                     /locus_tag="VN647_03480"
     CDS             3879..>4498
                     /locus_tag="VN647_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWG96124.1"
                     /translation="MSSEEQILRPDVQSPTSIKGKAIVAVVFLAAIGAFYFFDLMAYL
                     SLDVLKANRDKLFAFTEEHYVSAVALFILTYVAQTAFSLPGATLMTLTGGFLFGTVWA
                     ALYVNIGATTGATLAFLAARYLFRGWVERRFGNRLSAFQEGFTRNAFNYLLTLRLIPL
                     FPFFLVNLLSGLTRVRVGTYAAATALGIIPGSLAYTFAGRQLSTINS"
BASE COUNT          969 a         1234 c         1328 g          967 t
ORIGIN      
        1 tctggctcaa ccagcggccg gggtatctgg atgccggtca ttggggaatg ctgaaggtcc
       61 tggatcgtga cagtcgtgcc atcctgccgt taggcggtcg gatcccgacg actcagcagg
      121 ccgaagagcg atcgcacgcg aaatccagac cggtatccag gcaggccaaa taggccggtc
      181 cggcatcgaa tcggcggggg aacttggtca atgagtccgg gttccccccg atcgttctct
      241 tgtcgacgaa tgatgtcgtg aacccatcga ggacgagctg tagagcctga cccatgaagg
      301 gatgcggttt acccacatcg gttgagtggg tagtgttccg gctcatgccg attgtcatgg
      361 ccggttcggt tcaaacggcg acggcgcttg atttctcggc cgaacgaatc gtaaaaaacg
      421 gagagtccgt cgtcatcgct catgtcaacg ccaaggaaga tcgctggcgg ttcgagtacg
      481 cataccctca gcgaggcgtc agtatcatga tcgttcggat ggacaaacag cattcgtggc
      541 tcatcctctc gaagcggagg cagttcctcg aggtccctat cgcggccgac catgtgccga
      601 cggtgactga gaggatggaa ggagaaatct cgcgcgagtt tgtgggtgac gagacgctga
      661 gcggtcatcc cacggaatta tttgaagtca cggttgttgc gcagggtgaa actcggcaat
      721 attatagatg ggtgaccaag gcccaacgtt ttccgctcaa gaccgtgagt aagcagggga
      781 agtggtcgga ggagtttcgt cgggtgatct tcacggagca gtccaccttc ctcttcgaac
      841 ttccgcgaag gctggataac gccaatccgt ctgcaggcat gcaacattaa gaacgcgtgg
      901 ccggagggga tatgagacaa ccgtttccga gaggactgtg gatggccggc tgtgcgtcgg
      961 ttatcctgat attgggattt caagcagatg ccggagcgta tgaatcgggg aacgtggaag
     1021 atggtgcggt cgtgcggggc aaggtcacgt ttacaggaac cgagccggat ccgaaagagt
     1081 tcgagctgcg tcggtctccg gaccgagagt tttgcagcgc tgtgtcggat ggaaatgggc
     1141 atcggctcct gaaggaaatc actatcgggc cggacggcgg gcttaaagat gtggtggtgg
     1201 tcctggaggg cgtcgagaga ggcaagccgt tcacctttac cgatgccgaa gtggaagcaa
     1261 gtctctgccg gttcctgccg ttcgtcaccg tggtcagcga caagcgtcga gtcacggtgt
     1321 tcaatcgaga tccggtttta cacgatattc aagggtatgc gtacaatgaa gcaggcgtcg
     1381 atcttgtcct ccagcggcct gcgctgcatg agagcgggac taccgatatc gtgaaactgg
     1441 tcaaagggcg gaaagtgttt accatgcaat gcggcatgca tccctacatg cagaattggg
     1501 ggtacgcgat cgacaatccc tattatgcga tcacggacct caatggatca tttgccatcg
     1561 gtgacatccc ggcaggcaca taccacctca aggcctggca cccgatttta ggaactcaag
     1621 agcggactat caccgtgaaa ccgaacgaga ccatctcgct tgattggctg tttgacgcga
     1681 aacgagtcat cgaatgatca aaggtcccac gctctgtgtc tcgcgatcgt tttcggcatg
     1741 ccgacggtcg gtacggacga cgttgtggct gaggtctgtc ggcgggaaaa gcgagtcgtg
     1801 gtcatgctgg acaaggactt tggggacatc cgtgcctatc cgcctcatga gtatgcagga
     1861 ctcatcgtgt tgcgcgtcga ggaccaacag aagaaacgcc tggttgctct gatgaaccat
     1921 gttgtgaaca gactggcgac cgagcctatt caaggccgcc tctggatcgt agaggagcag
     1981 ggcattcgag ttcggatgag ttggtcggaa gcaaccgagt aatgcccgtt tcaacctttc
     2041 cctttcggca cgatcgggct cacggcattc tgatctctac ccgcctcata ggatttcaac
     2101 atccgtgtga tcgtcggcat cagttggtct gtgcgatcgg cgggcatttt aatccgcacg
     2161 ccgcgccctt cgttgcgccc caaggacaaa atgtggactt ccagcatcgc gtttcgcttg
     2221 tcgtcttgat aggtgaaggc taggcgcttc gcggggaatc ccgccagggt cgcctcctga
     2281 tccttctgcc aggtgacctg aacctgcttc ttgtcataca gatccgtgat gatgcgtcga
     2341 tgggcatcgg cgaattcttt gagtgtctgc cgtccgtcct ggctgcggcc gctgaccaca
     2401 ttcatgtggc cggatagtgc gaccaggctc tgctcgatcg gagggtacat tgtcactccg
     2461 attccgtccg gcaggggatt cccgaggcgc cagtcctctg gataacggac ggaaaaaccg
     2521 aagcgatcgt tcgtatagag cttccagccg gtcccctcgt tcgtgtcggt cacggcgccc
     2581 agagcaggcg tggcctgtgg gacgagactc atcgctacca gggcaaacac aagacatccc
     2641 cgaatcatca gcagcttcat aaggcagatt tatcctccat taccggatcg acttggcaca
     2701 gcgaaaccca acggtcgcag ttcgctgctc cggggcagcg ccgctccgcg tcgccgttcg
     2761 cagcaacggc ggagcgctct tccacgatcc accccgcacc accttatagc ggccttgccc
     2821 ggctccacac gggttacggt cgagcatagt cgcatagtaa tcgataccga accaatcttc
     2881 gacccattcg gcggcattcc ccgccatgtg atgtaggccg taggggcttc gaccctcctc
     2941 gccgcgttca acggacgcca caatgggtat ctcgtggaga tggtactgcc cgaacatagc
     3001 cagtccaatt gccggaggtt tgtgacccca gggaaaaaga taacccttct ctccccgggc
     3061 ggcctggccg gttctggcag tccccatgac aaaatgacct tggccgacgg ttatcatggg
     3121 ggaaccgtga gcgagcgtgg cgatgcgctc atagaatgag ttgtcctgcg ctcttccagc
     3181 caatggcttc aacaggagtg ctgtaaatgc caagaggaag agaagcctga aaggaatcgg
     3241 atcatgcccc cctggttttc tgtaccgaga acatcctcca ggagtcaatg gtcggagaga
     3301 gcagcggcat agtcccgatc ccagaaatcg ctccagagtt gttggatgta gtatgtggac
     3361 tgctgattat ccctctgttc attcaagcaa tagcggcttg aatcgtcggc tcatcgcgag
     3421 gatcaccaag ccggcgatgc cgccaaacag ccaggccagc atacgaacgg ggaaccacaa
     3481 gaaagctgca acggcttcag gcgtgggaaa ccgaaggccc aaggccgttt gctgaagccc
     3541 gtccggttga gagatgggat acagcgccat cgcaatcagc cccgatacat atccgacgac
     3601 gatcgagtac aggatggttg aggaaacggg aagcggtcca gtatgatgcc gacgcaacca
     3661 ctccagcagg aagatcgtca gcgtgatcaa gaaaatagtt tgataggaca caaagtagga
     3721 cccgcggatc caaaaattca tccagcggat catcaacgtg acaaatgcaa taagagaggt
     3781 caggacgaga cttaccaata ccagcatggc gcgcttcatg atgggtgagt ctcccaacat
     3841 cgtgacgatt actgtacgga gtcagacaga gggttgcaat gtcatctgag gaacagattc
     3901 ttagacccga tgtgcagagc ccgacctcga tcaagggaaa ggcgatcgtc gcagtcgtct
     3961 ttctggccgc gatcggcgcc ttttattttt tcgaccttat ggcctatctc tcgctcgacg
     4021 tgctcaaggc caaccgggac aagctcttcg cctttaccga agagcattac gtatcggccg
     4081 tggcattgtt catcctgacc tatgtcgccc agacggcctt ttcgttgccg ggagcgaccc
     4141 tgatgacgct gaccggaggg tttctcttcg ggactgtgtg ggccgcactc tacgtgaata
     4201 tcggcgccac gaccggggcc acactcgcgt ttctggccgc tcggtatctg tttcgtggct
     4261 gggtggagcg acggttcggc aaccggctct cagcttttca ggagggtttt acccggaacg
     4321 ccttcaatta tcttctcaca ttgcggctca ttccgctgtt tccgtttttt ctcgtgaacc
     4381 tgctctccgg gttgacgaga gtgcgcgtgg ggacgtacgc cgccgcgaca gccctcggca
     4441 ttatccccgg cagtctcgcc tacaccttcg ccggccgtca attgagtacg atcaattc
//
LOCUS       DATNDD010000145         3326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2529332, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000145 DATNDD010000000
VERSION     DATNDD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529332"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1039..1209)
                     /locus_tag="VN647_03485"
     CDS             complement(1039..1209)
                     /locus_tag="VN647_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96125.1"
                     /translation="MELSFCACGRWVVLRLDAQAARVLKNAFRTGVVDEVLAADQPFS
                     HRHFAPGAEAIG"
     gene            complement(1246..2160)
                     /locus_tag="VN647_03490"
     CDS             complement(1246..2160)
                     /locus_tag="VN647_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HWG96126.1"
                     /translation="MNRTAFIISMLGMVFLIAAPVQGEASAGSLDTKAIEQAIGKPGE
                     MKDDVYKVSLPRKDLSVSLNGVQLKPGFALGTWIAFKQAGHNAVLDGDLVLTEQEVGA
                     VFGQLRKVGIQVSALHNHLIGETPRVMFLHIEGKGDAGKMAMQVKEALSLTGTPMEPL
                     VASAITIEMSTASEEADFDAEVIQKELGHKGKIKDGVLHVSVPRPESIKMHGTTLPPS
                     MGMATAFNFQSAGGNKVAATGDFVMDRDDVNRVTKALAEHGILVTALHNHLVHGSPDL
                     YFMHFWADDSPEKVAKGLRAGLDAMKRG"
     gene            2959..>3326
                     /locus_tag="VN647_03495"
     CDS             2959..>3326
                     /locus_tag="VN647_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96127.1"
                     /translation="MDILILCRIVLMGWVAWIAFRPSSRRRKVKAGPPDHDSSSQKRA
                     GLTGEHTEVVDMPEPHSRPLTADEEHQAFTAGQPVWIRYEDTSGAVTERVVEIYRPCD
                     DEVIYTWCRRKQAPRTFVRR"
BASE COUNT          796 a          921 c          858 g          751 t
ORIGIN      
        1 ttgataagta ggagcagggc tgcgatggaa agagcttgga ggagtttcat catcattggc
       61 ctgatttatg aataaggata ccggctccaa gcctgccatg gcaacgctat cgaccctcag
      121 aaaaatggtc tagaagactc atggataacc cgaccagcga gccagagggt tgatgcgcac
      181 ccatggagaa gaggctcttc catagtatcc attgagatac gacacgtatc tgtccagata
      241 ccatacatac ataagatctg gagaaccaca tatccaactg ccaccagtca gactgttcaa
      301 caaggcctct ttttggacgc atgccattga ccattctatt tcggcaattg gcatagctta
      361 tgcggttctg aaggccagtc aacaagttca agatatcgac gagtgggagg gaatcatgac
      421 tcatccggga gatgtcgagg cagggaacat tcacatcata cgacagtacc ttgaaagatg
      481 gtttcgggat gttacgccgg acccgtatac tgagcgcttt gtctatcatg atggcacaca
      541 gacttcagag ccggcgctcg accgtgaatt cgtcaacgat ctgagcccgc agaggctcaa
      601 ggcctatatt gaacagacag tgctcccata ctccgtgcga gcgcagggaa gatcattcga
      661 gcaggggccg atggcatcac tgtccgggca agacagacgc cctgacccac cgcctaccga
      721 tacggaagaa cgttcacgtt catgagatgg ctgaagactg aagctacgtc aatgccggac
      781 tatacgaaac gccggatcga gttgaggcgt cgggagcagc atgcggatgg aacctggagc
      841 tgtcactgtg tcatttttga gttggaacca gagactggcg gtgcaagaaa gggcgtgctg
      901 acggcacctt cagaacccct gaagaggcag tagccgctgc gctgacacag gcgcaacaga
      961 ttatcgattc actccaaccc tcggcctcct ctctggagga acgcgatgcc tgatagaatg
     1021 ctcccaacgg tgaccgcact acccgatcgc ctctgcacct ggtgcaaagt gcctatgaga
     1081 aaacggctgg tcggcggcga gcacttcgtc cactacacct gtccgaaatg catttttcag
     1141 cacacgcgcc gcttgcgcgt ccagtcgtag caccacccag cgtccgcacg cacaaaaaga
     1201 aagctccatc ctctttcaag ggcggagctg ccctaaacga tgcactcaac cccgcttcat
     1261 ggcatcgagc ccagccctca gtcctttggc caccttctcg ggactatcgt cggcccaaaa
     1321 atgcatgaag tacaaatccg gcgatccatg tactaaatgg ttgtgcaatg ccgtgaccaa
     1381 gatgccatgt tcggcgagcg ccttggtgac ccgattcaca tcatcgcgat ccatcacaaa
     1441 atcaccggtc gcggcaactt tgttacctcc agctgattga aaattgaacg cggtcgccat
     1501 acccatgctt gggggcagcg ttgtcccatg cattttgatg gactccggtc tcggcaccga
     1561 tacatgcaac acgccatctt tgattttccc tttgtggcca agttcctttt gaataacctc
     1621 ggcgtcaaaa tcggcctcct cactggcagt gctcatctca attgtgattg cactggcaac
     1681 tagaggttcc atcggcgtac ccgtgagact taatgcctcc ttgacctgca tggccatttt
     1741 ccctgcatcg cctttacctt caatatggag aaacatcacc cgaggcgtct cgccgatcag
     1801 atggttatgg agggcgctca cttgaatgcc tacctttcgg agttggccaa aaacggcacc
     1861 gacctcttgt tctgttaaca ctagatctcc gtctaagacc gcattatgtc ctgcctgctt
     1921 aaacgcgatc caggttccga gggcaaagcc aggtttcagt tggactccat tcagagagac
     1981 cgataaatct ttgcgtggca gtgacacctt atagacatca tctttcatct ctccgggttt
     2041 gccgattgct tgctcaatcg cttttgtgtc gaggctgcca gccgatgctt ctccttgaac
     2101 aggcgctgcg atcaagaaca ccattcccaa catgcttatg atgaaggctg tgcggttcat
     2161 agtgtcctcc ttcacgttat aatcctatgg atagtctgcc atgtcccaac agttagggta
     2221 gcggggtaca tcactaggca atgggcctgc aaggtatcgc ggaaaatcgt tcatgagatt
     2281 gctctccgcg atggccgcta tgcccggcca aaacggctaa tcttgaactt atcctgaaag
     2341 ggtggctgta acgggaccac cagacaagga gatcgagcag cgaggcatcg cgttgtttga
     2401 gggtctcttc accgccctgt cacggatgag tcggccaacc cagatgacgt cactcatgtc
     2461 atcgaaccga tcggcgatcg cctgtgtgcg gtcggattcc tcgcccggct ttcctacagt
     2521 cgatgatgag cttctccttg gcgggattct caaactatgc cgccgcaaca tgcccttcaa
     2581 acggcatgca ccaggaagat ttttcgagag tgcaaagtcc gttgatcccg ttgggggaca
     2641 ccagttcatt tacatggccc atagcaagga gagatcgctg cgcggcgagg ttgtcctata
     2701 taggacacgc actctgtaac tcattgtaaa accttgctac tcatcggacc cttctaagat
     2761 atcatcttct tgatttcttc agtttggtct gtcctaaaat gacggcctat tcgatcgaca
     2821 tacctcgatt cagttaagat ggctatgtga acgaaatgct cggcgaagcc ccgaaggatt
     2881 tactgaagag gtaccgggaa aaagaaaaac ctaaatgaca cggagttttt aagctgcgga
     2941 atgccgccgc gagagcatat ggacattctc attctctgtc gcatagtgtt gatggggtgg
     3001 gttgcgtgga tcgcatttcg cccctcatcg cgccggcgca aggtcaaagc gggcccacct
     3061 gaccacgata gttcctcgca gaagagagct ggcctcacag gtgaacatac cgaggttgtc
     3121 gacatgccgg agccgcacag ccggccgttg actgcagacg aggaacacca ggcgttcacg
     3181 gctggccaac cggtctggat ccggtacgaa gacacatccg gcgcggtcac tgagcgagtc
     3241 gtcgagatct accgtccatg cgatgacgag gtgatctaca cctggtgccg gaggaagcag
     3301 gccccgcgca ccttcgtgcg tcgaaa
//
LOCUS       DATNDD010000146         4541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3067993, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000146 DATNDD010000000
VERSION     DATNDD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067993"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VN647_03500"
     CDS             complement(<1..592)
                     /locus_tag="VN647_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWG96128.1"
                     /translation="MEQQPRRKSWVVGPMIAVALLCGVVIGKGWERTGHAGETYEELK
                     TFSEVLNQVQKHYVDETKPKDLIQGAIRGMLSTLDPHSAYMTPEMYKEMQVETRGEFG
                     GVGIQIGVKDNRLAVIAPIEGTPAHRAGIKAGDFIVKVNDDPTKDLTLMDAVQKMRGP
                     KGSKVNLTIVRDGATDPLVFTLVRDTIKIESVKSKVI"
     gene            complement(661..1845)
                     /locus_tag="VN647_03505"
     CDS             complement(661..1845)
                     /locus_tag="VN647_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWG96129.1"
                     /translation="MRISFILLLWCLLVEGGWASVAGAANDPISEKIERERKTLETLK
                     DKIEEKRKRVEEAEKKRESVLQGIQSLDERLVHHRQDHHEISKKLRQKDREIEEITDR
                     LGAMRASIDARREAILARLRVQYMEGRFGRVKVLLTADSYGDFQRRGQYLSAVSHKDY
                     ELLETFRVDMERMQQAEHQRAEARAVLVAFKMNIEKKVADIRVLQKEKKVYLTKITHE
                     KDSYNRTVEELQRSASRVDGLLRDLETRRRATALHPPASSPLAHGVRGTLPWPADGKV
                     VSFFGRQKHPTFNTYVQRKGIEIRTAEGSLIHAVMTGSVVYADWLKGYGLVIILDHSN
                     GFFSLYAHASKILAKAGEQVAEGQPIGETGDTGMIGENTLYFELREGAEPVDPLHWLA
                     KR"
     gene            complement(1842..2741)
                     /locus_tag="VN647_03510"
     CDS             complement(1842..2741)
                     /locus_tag="VN647_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWG96130.1"
                     /translation="MRRLFYLIREASANMRINRTTTIVAVLTTAFTLACVGIFLLLYV
                     NLRHAAGSLQEDVKVMVYLDDRLPRERVQELERTLKIDRMVAEVFFISKEQALGEFRV
                     QFPSESHLLEGLGENPLPASFVVTLAPNFRSPDAMKGWVERVQTMEGVAKVDYNQEWL
                     SLLAELIGYIELAAIGVGILLSAAAVTIIGNTIRLALLARQEEIEILRSIGATRTFIR
                     IPYFLEGAVLGACGSALSLGILKVAFELFRQQIQLTGRFSGIGDMISFFPLSVCLALV
                     MAGMGLGFAGSVVSLLRVGQGRA"
     gene            complement(2738..3427)
                     /gene="ftsE"
                     /locus_tag="VN647_03515"
     CDS             complement(2738..3427)
                     /gene="ftsE"
                     /locus_tag="VN647_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWG96131.1"
                     /translation="MDAIIQLIHVSKWHDRKAALSDVTLEIEKGEFVLLMGSSGAGKS
                     TLLRMLIGEEQPDEGQIFVHGRNVTKIRPAEIPYLRRKVGAVFQDFRLLPKKSVFDNV
                     ALPLIVQGASEKDVRRKVTEALRAVDVDHKKDQLPNSLSTGEQQRVCIARAIVNGPVV
                     LLADEPTGNLDPERTREIIELFKLINARGTTVIVATHDPHVLNHMNRRVITLVQGVLL
                     SERQIRQGIGV"
     gene            complement(3448..3837)
                     /locus_tag="VN647_03520"
     CDS             complement(3448..3837)
                     /locus_tag="VN647_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWG96132.1"
                     /translation="MATSDPRHQFGQASEMQAEQFLLAKGYRILDRNVRTPLGELDLV
                     AEDQGVVVFVEVKGRTTEAFGGALLAVNHRKRMKLAKLATLYLAQRHWSNKACRFDVV
                     LVQGRPSDRGRIEHLQNAFDVGEHGKF"
     gene            complement(3842..>4541)
                     /locus_tag="VN647_03525"
     CDS             complement(3842..>4541)
                     /locus_tag="VN647_03525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWG96133.1"
                     /translation="SRQCDGMIGEGMYDRPTEEFERTARLRGYRRIAGIDEAGRGPLA
                     GPVVAAAVVLPTRCRLLGVDDSKQLSEGKRDRLYAAILEQALGVGIGSADVGEIDRLN
                     ILEATRLAMRRAIDQLAPPPDYLLIDAVTLPGIRISVRPIIKGDSLSLSIAAASIIAK
                     VTRDRLMAKYHEMFPEYDFLSHKGYCTTGHLQRLARHGPCSIHRRTFMPVHEMIIPAK
                     RESARRMLPTLFEQ"
BASE COUNT          989 a         1362 c         1134 g         1056 t
ORIGIN      
        1 cgatgacctt cgacttcacg ctttcaattt tgatggtgtc ccggacgagg gtgaacacca
       61 agggatccgt tgccccgtcc cgtacgatgg tgagattgac cttactgcct ttcggcccgc
      121 gcatcttctg gacggcatcc atcaacgtca gatccttcgt ggggtcgtcg ttgactttaa
      181 cgatgaaatc gccggccttg atcccggctc gatgcgcagg cgtgccttcg atcggcgcga
      241 ttacggccag ccggttgtcc ttcacgccga tttgaatacc gactccgccg aattctcccc
      301 tggtttcgac ctgcatctcc ttatacattt caggagtcat gtacgctgag tgcggatcaa
      361 gcgtcgacag catgccgcga atggctcctt gaatgagatc cttcggcttc gtctcatcga
      421 catagtgctt ttgaacctga ttcaagacct cggagaaggt tttgagttcc tcataggttt
      481 caccggcgtg accggttcgc tcccagcctt tcccgatgac cacgccacag agcagagcga
      541 ctgcgatcat cggtccgacc acccaagatt tccgccgcgg ctgctgttcc ataatcttca
      601 catcccttct ttcctgcgtt gtcgaaggct cggacccgat cgacaccgac aacacacgtc
      661 ctatcgtttt gccagccagt ggagcggatc aaccggctcg gccccttcac gcaactcgaa
      721 gtataaagta ttttctccaa tcatgcccgt gtctcctgtc tccccgatgg gttgaccttc
      781 ggcaacttgt tcacccgctt tagccagaat cttggacgca tgcgcataca gagagaaaaa
      841 cccattggag tgatctaaga ttataacgag tccgtatcct ttcaaccagt ccgcatagac
      901 gacgcttcct gtcatgacgg catggattaa gcttccctct gcggtccgaa tttcaatgcc
      961 tttgcgctgg acatacgtat tgaacgtcgg gtgtttctga cgcccgaaga acgacacgac
     1021 ttttccatcg gccggccaag ggagtgttcc tctcacgcca tgggctaagg gtgaagacgc
     1081 cggagggtgg agggccgtgg cacgtcggcg cgtttccaaa tcacgcaata aaccatccac
     1141 tcgcgaggcg gaacgctgca gttcttcgac cgtgcgattg taggaatcct tttcgtgcgt
     1201 gatcttcgtc agatagactt tcttctcctt ctgaagcact cggatatcgg ccactttctt
     1261 ctcgatgttc atcttgaaag cgaccagtac ggccctggcc tcggcacgct gatgctcggc
     1321 ctgctgcatc cgctccatat cgacccgaaa cgtctccagc aattcatagt ccttgtgtga
     1381 aacggcagag aggtattgcc cgcgacgctg gaaatcaccg tacgagtcgg ccgttaagag
     1441 aaccttcaca cgcccaaacc gtccttccat atactgcacg cgaagccggg caagaatggc
     1501 ctcacgtcgc gcatctatgc tggctcgcat cgcccccagc ctgtctgtaa tctcttcgat
     1561 ctcccgatcc ttctgtcgca gtttcttgct gatctcatga tgatcttgcc gatgatgcac
     1621 cagccgttca tccagagatt ggataccttg cagaaccgat tctcgttttt tttccgcttc
     1681 ctcgacccgt ttccgtttct cttcgatctt gtccttcagc gtctcaagcg tctttctctc
     1741 acgctcgatt ttttcggaga tcggatcatt cgccgcaccc gctaccgatg cccaccctcc
     1801 ttccaccaaa aggcaccaga ggaggaggat gaaagagatt ctcatgcccg cccttgcccc
     1861 acgcggagga gcgacaccac gctaccggca aaaccaagcc ccattcccgc catgacgagc
     1921 gccagacaca ccgaaagggg aaaaaaagaa atcatgtctc cgatgccgct gaaacggcct
     1981 gtcaattgaa tctgctgtcg gaacaattca aacgctacct tgagaattcc caacgacagt
     2041 gcgctgccgc atgccccgag cacggctcct tcgaggaaat atggaatacg gatgaacgtg
     2101 cgcgtggctc caatcgagcg gaggatttcg atctcctcct gtctggccag cagtgcgagc
     2161 cgaatcgtat tcccgatgat cgtcaccgcg gctgcagaga ggaggatacc caccccaatc
     2221 gcagccagct cgatgtatcc gatcagttcg gccagcagac tgagccattc ctgattatag
     2281 tcgaccttgg cgacaccttc cattgtttgc actcgttcaa cccagccttt catggcgtcc
     2341 ggagaccgaa agttcggcgc aagcgtcacc acgaacgatg cggggagagg gttctcaccg
     2401 agcccttcaa gcaagtgaga ctcactgggg aattgaactc gaaattctcc caacgcctgt
     2461 tctttcgaaa taaaaaacac ctcggcaacc atacgatcga tcttgagtgt tcgctcgagt
     2521 tcttgcaccc tttctctggg aagtcggtca tccaaataaa ccatgacctt gacgtcttcc
     2581 tggagcgatc ccgctgcgtg ccgcagattc acatagagca acagaaagat gccgacacag
     2641 gccaaggtaa atgccgtggt cagaacggcc acgatcgtcg tcgtgcgatt aatccgcatg
     2701 tttgccgacg cttcgcgaat gagataaaac aatcgtctca tactccgatc ccttgccgga
     2761 tctgcctctc cgacaacaac accccttgca ccaaggtaat cacccgccga ttcatgtgat
     2821 tcagaacatg gggatcgtgc gtcgccacaa tcacggtcgt ccctctggca ttgatcaatt
     2881 tgaacaactc tataatctct cttgtgcgtt caggatcaag gttgcccgtt ggttcgtcgg
     2941 ccaggagcac caccggcccg ttgacgatcg cccgtgcaat gcacacacgc tgctgctctc
     3001 ccgtggagag actgttcggc aactgatctt ttttgtgatc gacatcgact gcgcgcaatg
     3061 cctccgtgac tttacgacga acatctttct cggaggcacc ttgaacgatc aacggaagcg
     3121 ccacattgtc gaagacggat ttcttcggca aaagacgaaa gtcttgaaat accgcgccga
     3181 cttttcgccg taggtaagga atctcggccg gtctgatctt cgtcacattc ctgccatgaa
     3241 cgaaaatctg tccttcatca ggctgttcct caccgatcag catgcgcagc agcgtagact
     3301 tcccggcacc gctcgatccc atgaggagga caaactcgcc cttttcgatc tcgagcgtga
     3361 catcggacag cgccgctttt cgatcatgcc atttggacac gtggatgagt tgaatgatgg
     3421 catccatgga aaccctttgg agaagggcta gaactttccg tgttcgccga cgtcaaacgc
     3481 gttctgaaga tgttcgatcc ttccccggtc ggaaggtctc ccctggacca acaccacatc
     3541 gaatcggcag gccttattgg accaatgccg ttgcgctaaa tatagggtgg ccagtttcgc
     3601 cagcttcatg cgcttgcgat gattcaccgc cagcagagca ccgccgaagg cttccgtggt
     3661 tctgcccttg acctcgacaa agaccacaac accctggtct tctgccacca ggtccaattc
     3721 gccgagagga gtccgtacgt tgcgatcgag aattcggtag cccttggcca acaagaactg
     3781 ctcggcctgc atttcgctgg cttggccgaa ctgatggcgc gggtcggaag tggccatgcg
     3841 cttactgttc aaacaatgta gggagcatcc gtctggccga ttcccttttc gcagggatga
     3901 tcatctcatg caccggcatg aatgttcgac gatgaatgga gcacgggccg tgacgcgcca
     3961 atcgctgcag atgtcctgtc gtgcaatacc ccttatgcga gagaaaatcg tattcaggga
     4021 acatttcatg gtacttcgcc atgagacggt ctctcgtgac tttggcgatg atggaggcag
     4081 ccgcaatcga gagggaaagg gagtctccct tgatgatggg ccgtacggag attctgattc
     4141 caggaagcgt cacggcatca atgaggagat aatcaggagg aggagccagc tgatcaatgg
     4201 ctcgccgcat cgctagccgg gtcgcttcga gaatattgag ccgatcgatc tcaccgacgt
     4261 ccgccgatcc gattcccact cccagagctt gctcaagaat cgcggcatat aatcggtctc
     4321 gttttccttc agaaagttgc ttggaatcat cgacgcctaa gagtcgacat cgggtcggta
     4381 agacgacagc ggcggcgacg accggacctg ctaatggacc gcgtccggct tcatcgatac
     4441 cggcaatacg acgataccca cgcagccggg ctgtccgctc gaactcttcg gtgggtctat
     4501 catacatccc ttcgccgatc atgccatcgc attgccgtga g
//
LOCUS       DATNDD010000147         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1637863, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000147 DATNDD010000000
VERSION     DATNDD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637863"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VN647_03530"
     CDS             complement(<1..815)
                     /locus_tag="VN647_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWG96134.1"
                     /translation="MIIGIPKEIKDHEYRVSLTPEGAATLRQSGHEVWLEPSAGQGSG
                     FSDDEYRKAGALIARSKNEVFEKADMIVKVKEPLLSECPLFRPGQVLFTYLHLAALPE
                     LTKALLDMKLTAVARETIEAKDGSLPMLKPMSEIAGRMSVQIGAQYLEKTHGGCGLLL
                     GGVPGVAPATVTVLGAGVVGSAAIRIAVGMGAQVRVISLDVEQLRYLDDQYQGRIVTL
                     TSTTAAIEDAVQSADLVIGAVLIHGAKAPKLVSRSLVSRMKPGSVIVDVSVDQG"
     gene            977..1360
                     /locus_tag="VN647_03535"
     CDS             977..1360
                     /locus_tag="VN647_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96135.1"
                     /translation="MDSWNESCQACGAAGVPLTKLSLGKDFFGRPYDRLSPSSDQNPK
                     WYCALCSVHKNLQRDFRDIRAEFDKLRAGQGSELSKGDEFRRASLRLQEIMAILKGTQ
                     QQSPFLNGRDITLLMERLNTLTMPV"
     gene            1452..1727
                     /locus_tag="VN647_03540"
     CDS             1452..1727
                     /locus_tag="VN647_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWG96136.1"
                     /translation="MGSETLHAHFKSETKRLNLKGLVRANKAGCLDHCELGPSVVVYP
                     EGVWYWVGTEADVTEIMERHVLKGEIVTRLLMPDHPVPERLDPIKKS"
     gene            1777..>2122
                     /locus_tag="VN647_03545"
     CDS             1777..>2122
                     /locus_tag="VN647_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96137.1"
                     /translation="MEKVAFTQQFPGLLLDWKSFEGKTIQAVIPLTGKPSAAVIICSD
                     ATFTVVPALSPEPWALGQGIVDARARLEPAHQAAYKEYDRLTKNDKEALRIARLEKIL
                     ARFTIIWSRFLSS"
BASE COUNT          508 a          626 c          538 g          450 t
ORIGIN      
        1 ccctgatcga cagaaacgtc cacaatcacc gaccccggct tcatccgtga gaccagcgaa
       61 cgggacacca gcttgggggc cttggcgccg tggatcagca cggcaccgat gacaaggtca
      121 gctgattgca ccgcatcttc aatcgccgct gtcgtcgacg tcaaggtcac gatccgtcct
      181 tgatactgat cgtcaagata cctcagttgt tccacatcca agctgatgac tcgaacttgg
      241 gcccccatgc caactgcaat acgaatcgcg gcactcccca ctactccggc accaagcacc
      301 gtgacagtcg ccggtgcaac gcctggaact cctcccagca acaacccaca ccctccatgc
      361 gtcttttcca gatactgcgc accgatctgc accgacatgc ggcctgcgat ttcactcatc
      421 ggcttcaaca tcggcagcga cccatccttg gcttcgatcg tctcacgggc aacggcggta
      481 agcttcatat caagcagtgc cttagtcaat tccggcaaag ccgccagatg cagataggtg
      541 aacaagacct ggccaggacg gaacagtggg cattcagaga gtaagggttc cttgaccttc
      601 acaatcatat ctgccttttc aaacacctcg tttttggaac gagcaatcag tgccccagcc
      661 ttacgatact cgtcatcgct gaatccacta ccctgcccgg cggacggttc gagccagacc
      721 tcatgcccgc tctgacggag cgtcgctgca ccctccggcg taaggctgac gcgatactca
      781 tgatctttaa tttctttcgg aatgccgatg atcataacgg ttcccctttc ctgcgacgag
      841 tgctgcaaac tcgatactgc cattatacct cgatacccga agtgatcggg cctgaccgcc
      901 gtacatacag ccgtggacag tctggaaaga cctgttgtaa gatgaagaaa cttcactgtc
      961 atttatggag gtgaggatgg actcctggaa cgaatcgtgt caggcatgcg gagccgccgg
     1021 tgtcccgctc acgaagcttt cgctggggaa agattttttc ggccggccgt acgatcgtct
     1081 gtcgccatca tcggatcaga acccgaaatg gtactgtgcc ctttgttcgg tacacaagaa
     1141 cttgcagcgg gattttcgcg atatccgggc cgagttcgac aaactacgtg ccggccaggg
     1201 atcggaactc tcgaaaggcg acgagtttcg acgcgcgtca ttgcggttac aggaaatcat
     1261 ggccattttg aagggaaccc aacagcaatc tccgttccta aacggccgag atatcacgct
     1321 actgatggaa cggctcaata ccctcaccat gccggtatag ctgccatctc cctatgccac
     1381 catttcaacg tcacatcttc gtgtgtacaa accagcgtcc taaagacgac ccgcgcggct
     1441 gttgtgcgaa cttgggctcg gaaacacttc atgcgcactt caagagcgaa accaagcgat
     1501 taaacctcaa gggtctcgtt cgtgccaata aggccggttg ccttgaccac tgcgaactgg
     1561 gtcctagtgt agtggtctat cccgagggag tgtggtactg ggtcggcaca gaagccgacg
     1621 tcacagagat catggagcgg catgttctga agggcgaaat tgtcacccga ttgctcatgc
     1681 ccgatcatcc ggttccggaa cgactggacc ccatcaagaa atcgtaggat ttactactct
     1741 acttccatgc cgacagaaga caaatggaag gccaatatgg aaaaagtggc cttcacccag
     1801 cagtttcctg ggctgctgct ggactggaag tcgttcgaag gcaagacgat ccaggccgtg
     1861 attccattga ctggaaaacc cagcgccgcc gtgattatct gcagcgatgc aacctttacc
     1921 gttgttccgg cgctttcacc tgaaccctgg gcactcggac agggcatcgt cgatgctcgc
     1981 gctcgacttg aaccggcaca ccaagcagct tacaaggagt atgatcgatt aacgaagaac
     2041 gacaaggagg cgctgagaat cgccagactg gaaaagattc tggcgcggtt cacaataatc
     2101 tggagcagat tcctgagctc aa
//
LOCUS       DATNDD010000148         3879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_318090, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000148 DATNDD010000000
VERSION     DATNDD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318090"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..988)
                     /locus_tag="VN647_03550"
     CDS             complement(293..988)
                     /locus_tag="VN647_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWG96138.1"
                     /translation="MVTISVIIPALNEEKTIRQTLSCLPTAEILEAIIVDGGSTDQTI
                     SIAEAFCAEASNARIITAPVGRARQMNEGAKAGRGDILLFLHADTRLPTKAILMIEAA
                     LTDPAVVGGRFDVTFDSRSVWGTMISTFMNRRSRLSGIATGDQALFVRRRIFEQLGGF
                     ADIPLMEDIEFSRRLKRTGQMAALHQTVTTSFRRWEQQGPLRTILSMWALRFMYWAGV
                     NPTRLSRWYKTVR"
     gene            complement(994..1407)
                     /locus_tag="VN647_03555"
     CDS             complement(994..1407)
                     /locus_tag="VN647_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96139.1"
                     /translation="MAPSDLPPQGEPPRSSRPAKAGEQGGGGNEHHIHVIGLMVGSAL
                     MFIGFLNVFLSISGGFEINVMPFLVYFGGLAIWANAAIETPALRYGTMAVAISLALGL
                     FQYGEVLFWHKQVLFWVTIIVVMYFMFVEPKKPTP"
     gene            complement(1555..2712)
                     /locus_tag="VN647_03560"
     CDS             complement(1555..2712)
                     /locus_tag="VN647_03560"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HWG96140.1"
                     /translation="MTTHSPRAGILPYQDIQRLIASRAISASPTVEERQIQPASLDLR
                     LGRKAYRLISSFLPELSAISSRLDVLDFYQSDLVMYEMDLTDGAILEKGHVYLVPLLE
                     QLALPKTIRARANPKSTTGRLDVFTRVVTDLTSGFDEIRAGYRGQLFLEVVPRSFAIK
                     VRTGQSLNQIRFVRGEATVPDQALQALHRHTPLLYHNTASHKTIDNRDFRVERGLFLR
                     IDLAGTDQRDTRVIGYRAKKNSHVIDLEKVGHYAAADFWEPLHRHRHDSLLLEPEEFY
                     ILASKERIRVPAGYAAEMVAYEAACGELRTHYAGFFDPGFGYGSKGEIKGTQVVLEVR
                     PHDVPFLIHDGQTFFKVVYDKMMGMPAQLYGSPLGSSYQRQTLTLSKHFKV"
     gene            complement(2709..>3879)
                     /locus_tag="VN647_03565"
     CDS             complement(2709..>3879)
                     /locus_tag="VN647_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HWG96141.1"
                     /translation="AQSIVKPKDLAERFGLDEKEIEAIVGPYPMRITPTVLETIKSKD
                     DAIWKQVVPNTAELDDIAADEDPLEEDLMSPVPHLVHRYPDRVLLMVTNQCPIYCRFC
                     TRKRLVGKPGFLKKGELDRAIQYLREHKEVRDVILSGGDPLLLPDHLLERVLKALRTI
                     PHLELIRIGSRVPGSLPQRITSKLCEMVRRYHPIYMNLHFNHPDELTPEVKAACGMLA
                     DAGIPLGAQTVLLKGVNDDPEIMKRLVHQLLLARVKPYYLYQADLTKGTNHFRTTVET
                     GLNIIKALQGHTSGMAVPHFVIDAPGGGGKIPLLPDNYVVHLDEDGAILRNYEDKTFH
                     YPQPKSDNRRELPMIGVQSPLDDPEEAYAACSDSYPDRDGYAAWGNSCGGDADDL"
BASE COUNT          825 a         1068 c         1133 g          853 t
ORIGIN      
        1 gccagatagc gatcgatcgg cagcttcagt tttgaagcgg ccgtctttgt tctttccaaa
       61 gtatcgagca cgaaactgcc atggagcgtg gccgcttcgt ccggagtcag tggtgggcag
      121 agacgcgtct tcacctgacc cgggatcggt gctttggcga agatcaccaa ggccgcgtga
      181 ggcgtgaggc gtgaggcgtg aggcgatttc ggatcggagg cgttgtcggt ttctgggcgt
      241 cttccgttct atctttcaca ccgctcaccc cttacgcctt actcctcacg ttttatctga
      301 cggttttgta ccatcggctc agacgcgtag ggttgacgcc tgcccaatac atgaatcgca
      361 aagcccacat cgagaggatg gtccgcaagg ggccctgttg ctcccagcgt cggaacgatg
      421 tcgtgaccgt ctgatgcagt gccgccatct gtccggtacg cttcagtctc cggctgaatt
      481 caatgtcctc catcagcggg atgtcggcga agcctccgag ctgttcaaag atccggcggc
      541 gtacgaatag ggcctgatcg cccgtggcaa tgccgctcaa tcgggaacgc cggttcatga
      601 atgtgctgat catggttccc cagacggagc gagagtcgaa ggtgacatcg aagcggcctc
      661 ccaccaccgc cgggtcggtg agcgccgcct cgatcatcag tattgctttg gtcggcaatc
      721 gtgtatcggc atgcaagaag aggaggatgt caccgcggcc ggccttcgcc ccctcattca
      781 tttggcgtgc ccgccctact ggagcagtta tgatacgagc gtttgatgct tccgcgcaaa
      841 aggcttcggc aatcgaaatc gtctgatcgg tgcttccccc atcaacaatg atggcttcga
      901 ggatttcagc cgtcggtagg catgagagtg tctgcctgat ggtcttctct tcattcagag
      961 ccggaatgat gacggagatc gtcaccatgg aggtcacggt gtgggtttct tgggctcgac
     1021 gaacatgaaa tacatgacga cgatgatggt gacccagaag aggacctgct tgtgccaaaa
     1081 gagaacttct ccatactgaa agaggcctaa ggccaaggag attgcaacgg ccatggttcc
     1141 atagcggaga gccggagttt cgatggcggc attggcccag atcgcaaggc cgccgaaata
     1201 gacgagaaac ggcatgacat tgatttcgaa gccgccgctg atggacagaa acacattgag
     1261 aaagccgata aacatcagcg ccgatccgac catcaagccg atgacgtgta tgtgatgctc
     1321 gttcccacct cctccctgct ctcccgcttt ggccggacgt gacgagcgtg gaggttcgcc
     1381 ttgaggcggc agatcggacg gagccatggt taacagtctc cacatagttt cgcaggatgt
     1441 tcaaaaaggc cgtccagcga ggccgcagcg agcgaagagg cgaggcgtac gctccggtac
     1501 gttgagactc tgagcgacgc gagaacgaag ctggcgggct ttttgaacat cctgctagac
     1561 tttaaaatgt ttactgagtg tcaaggtttg tcgctgatag gatgatccca acggggaccc
     1621 atagagctgg gccggcatcc ccatcatctt gtcatagacg accttgaaaa aggtctgtcc
     1681 atcatgaatc aagaatggca cgtcatgcgg ccgaacttcc agcacaacct gggtgccttt
     1741 gatctctccc ttcgatccat agccgaaccc cgggtcgaaa aagccggcat agtgagtccg
     1801 tagctctccg caggctgctt cgtaggccac catttcggcg gcatagccgg ccggcacacg
     1861 aatgcgctcc ttcgaggcaa ggatataaaa ctcttccggc tccaagagca agctatcgtg
     1921 gcgatggcga tggagcggtt cccaaaaatc agccgcagca taatggccga ctttctcgag
     1981 atcgatgacg tggctatttt tctttgctcg ataaccgatc acacgggtat cgcgctgatc
     2041 cgtgccggca agatcgatgc ggaggaacag tccgcgttcg acacgaaagt ctcggttgtc
     2101 tatagtctta tgggatgccg tattgtgata cagcaacggt gtgtgtcgat gcagcgcctg
     2161 aagtgcttgg tccggcaccg tagcctcgcc gcgcacaaat cggatctggt tcagcgactg
     2221 ccctgtgcgg actttgatcg caaacgagcg tgggacgact tccaggaaga gctggcctcg
     2281 atagccggca cggatttcat cgaatccgga ggtcagatcc gtcaccacgc gggtgaagac
     2341 atccagacgg cccgtcgtgc tctttggatt cgcccgggca cggatggtct tcgggagggc
     2401 gagttgttca agcaatggaa cgagatacac atggcccttc tccaaaatcg ccccgtcggt
     2461 cagatccatc tcgtacatca cgagatccga ttgataaaaa tccagcacgt ccagtctaga
     2521 ggagatggcc gacagttccg gcaaaaagct gctgatcagc cgataggctt tgcggcccag
     2581 acgaagatcg aggctggccg gctggatctg tctttcttcg actgtcggag aagcgctgat
     2641 ggctcgactt gcgatcaagc gctggatgtc ctgatagggg agaataccgg ctcgcggcga
     2701 atgagtggtc acaggtcgtc cgcatctccg ccgcagctgt tcccccaggc ggcgtaaccg
     2761 tctcgatcag ggtagctgtc gctgcaagcc gcataggctt cttccggatc atcgagcggg
     2821 gactgtacac cgatcatcgg aagctcccgc cgattatcgg acttaggctg aggatagtgg
     2881 aatgtcttgt cctcataatt ccggaggatc gccccatcct catcaagatg gaccacgtag
     2941 ttgtcgggca gcaacgggat cttgccgccg ccgcccggcg catcgatcac gaaatggggc
     3001 acggccattc cgctcgtatg gccctggagc gctttgatga tgttcaggcc tgtttcgacg
     3061 gtcgtccgga aatgattcgt tcccttggtc aagtctgctt gatacagata atagggcttc
     3121 acccgggcga ggagcaattg atggacgagg cgcttcatga tttccggatc gtcgttcacg
     3181 cctttgagga gaaccgtttg ggcaccgagc ggaataccgg catccgcgag catgccgcag
     3241 gcggctttca cctcgggagt cagctcatcc gggtggttga aatgcaggtt catataaata
     3301 ggatggtacc ttctgaccat ctcacacaat ttggacgtga tccgttgggg taatgacccg
     3361 gggacccgcg agccgatccg tatgagctcc aagtgaggaa tcgtccgaag cgccttcagt
     3421 acacgttcga ggaggtggtc gggaagcagc agcggatccc ctcccgagag aatgacatcg
     3481 cgtacctcct tgtgctcgcg aaggtactga atggctcggt ccagctcacc ttttttgagg
     3541 aagccgggtt ttcccaccag tcgctttctc gtacaaaacc gacaatagat cggacattga
     3601 ttcgtcacca tgagcagcac ccggtctgga taccggtgaa caaggtgagg caccgggctc
     3661 atgagatctt cttcaagggg atcctcgtcg gccgcaatgt cgtccaactc tgcggtattg
     3721 gggacaacct gtttccagat cgcgtcatcc ttcgatttga tggtctcaag taccgtcggg
     3781 gtgatccgca tggggtaagg gccgacgatc gcttcaatct ctttctcatc aaggccgaat
     3841 cgttcggcca agtccttggg cttcacgatg ctctgagcc
//
LOCUS       DATNDD010000149         4481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4269596, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000149 DATNDD010000000
VERSION     DATNDD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269596"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..894)
                     /locus_tag="VN647_03570"
     CDS             complement(202..894)
                     /locus_tag="VN647_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWG96142.1"
                     /translation="MANESPNRKILRIGHRGACGHAPENTLASIEQAIALQCAFTEVD
                     IQRTSDDALVLLHDKRVDRTTNGRGRLSELTLPDIRKLDAGGGQMVPTLKEALRAANG
                     RIGVILELKTGGLAYDVCATVRASGFNRPVIYASFLQKELHHVRRVDPNSQTLVLFNK
                     WFPKDPVAAAFKLQATHVGLHFNTATRHRVNALHNAGLTVFVYTVNKPAEINKMNLLG
                     VDGLISDFPDRI"
     gene            875..1915
                     /locus_tag="VN647_03575"
     CDS             875..1915
                     /locus_tag="VN647_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWG96143.1"
                     /translation="MGLSFAMALSYHIAVAEKKVKRAPLPKGVRRRGKPFGSSTGLAS
                     IELQAAAPPGVVAELHQAVEQDGGKVLSVYRDPYGGQWLVLAALPIELVEPTPYQRNL
                     SDSHVRKLEAAIGKIGRFLDPIITVRIAKSDHMAKYWTPNGNHRLSAMRTLGAKSIIA
                     IVVPEAAAAYQILALNTEKAHNLREKALEVIRMYRQLVQLDHATEAVYALEFEEPALI
                     TLGLCYEERPRFSGGAYHPVLKRVDEFLQKPLHAALSIRQQHAKTVLALDDLIVKQVE
                     TIKAKGLTSPYLKSFVVARVNPIRFRPKDAPPLSFDDALDRMTQAAEKFNPDKIKMDD
                     LAKSGGVPDENE"
     gene            2055..2525
                     /locus_tag="VN647_03580"
     CDS             2055..2525
                     /locus_tag="VN647_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96144.1"
                     /translation="MAIKLHATWLLLGALIAPLPLWADANLDYQNRGDRFEGVKLKPV
                     SGYDIKVISVLADYREPAERLPDQLRVGFYLQSQTQVVGIEGGDQNGLAALRVVGPIP
                     DGVVALPLDLMTNVVGGEALTFIGYVPRGPMESLAAGGPDTQGASGQSSGARHS"
     gene            2718..3065
                     /locus_tag="VN647_03585"
     CDS             2718..3065
                     /locus_tag="VN647_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWG96145.1"
                     /translation="MSLADNKCIPCRGGVPPLPNDRTQALLKELGRGWSLNGQGHLER
                     LYTFKDFAQALEFVNKVGGVAEAEGHHPDLYLAWGKTKVEIWTHKINGLTESDFYMAA
                     KADREFEPFRAGT"
     gene            3321..>4481
                     /locus_tag="VN647_03590"
     CDS             3321..>4481
                     /locus_tag="VN647_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWG96146.1"
                     /translation="MPSKSEGQQPSLVHGRQPQSTGAITDRQQSLDNADEPKAPRRPF
                     EQESSTETSRSELPDAPVGSFSPESTTEGPDADPPSESDEDQPGASTRPARSKQSPPY
                     LIIGAALLVVMGAFVGQWILSPTLSSILTKNPSAFQTMESRRSQSQGMTPDPAPAPIV
                     LVPAGEFMMGARQEYGMADKDERTIHTVYLDAFYIDQYEITTARYAKFFQETKRSAPK
                     YWSEQVLKQHERKPVVGVDWNEAVAYCSWAGKRLPTEAEWEKAARGTDQRLYPWGNEE
                     PGRERANFDRCCDSSHYGILTDVGSFDGGKSPYGVYDMAGNVWEWVADWSDGGYDATS
                     LERDPTGPSRGEKRVVRGGSWDSAPTYVRSSYRLRLSPTFRLDNIGFRCARDV"
BASE COUNT          991 a         1218 c         1309 g          963 t
ORIGIN      
        1 acacaagcac ctgggattga cgaggtctcc cccgattgga aaccttgggt cacccacagt
       61 atcgtgcggg tcttgagggc cgcgacaaca gacatcaata ataattatat atggcgtcct
      121 aagcgccaac tcgcgggcga cctcccaccg tagtgcaagg tcgcccgcgc tgtttctgaa
      181 cccttcccct cgtcgtgccg atcagatacg atccgggaaa tccgagataa gaccgtctac
      241 accgaggagg ttcatcttgt tgatctcggc aggtttgtta acggtgtaga cgaatacggt
      301 caaccccgcg ttgtggagtg cgttcactcg atgacgtgta gcggtgttga agtgtagacc
      361 aacatgtgtg gcttgtagtt tgaaggctgc tgcgacaggg tccttgggaa accatttgtt
      421 gaacaggact aacgtctggg agttcggatc aactcgtcgg acatggtgaa gttctttctg
      481 tagaaacgaa gcatagatca caggtcgatt gaagccgctg gcacggacgg tcgcgcagac
      541 atcataggcc aacccccccg tcttcagctc cagaatcacg ccgattcggc cgttggcggc
      601 cctgagagcc tccttcaacg tcggaaccat ctggccgcca cccgcatcca gttttcgaat
      661 atctggaagc gtcagctcag aaaggcgccc gcgaccgttc gtggtgcggt ctacccgctt
      721 gtcatgcagg aggaccagcg catcgtcgga agttcgctga atgtccactt cagtgaaggc
      781 acattggagc gcgattgctt gctcaatgga cgccagggta ttttccggtg cgtgcccgca
      841 ggcgccgcga tggccgatgc ggagaatctt tcgattgggg ctttcatttg ccatggctct
      901 gtcctaccat atcgccgtgg ctgaaaagaa agtgaaaaga gcacccctcc ccaagggggt
      961 gagacggcgt gggaaaccgt ttggatcctc gaccggcctt gcttcaattg agttgcaagc
     1021 tgccgcaccg ccgggtgtcg tggcggagtt gcatcaggcg gtcgaacaag atggagggaa
     1081 agttctctcg gtctatcgtg acccatatgg aggacagtgg ttagtcttgg cggccctgcc
     1141 gattgagctc gtcgagccga caccatatca gcgaaatctt tccgactctc acgtcagaaa
     1201 gttggaagcg gcgatcggga aaatcggtcg ctttctcgac cccatcatca ccgtgcgaat
     1261 cgcaaaatca gaccatatgg cgaagtactg gacaccgaac ggcaaccatc gcctgtccgc
     1321 gatgcggacc ctgggagcga agagcattat cgcgatcgtg gttccagaag ctgctgccgc
     1381 ctatcagatt cttgcgctga ataccgagaa agcacacaac ctccgtgaga aagcgttgga
     1441 agtgatccgg atgtaccgcc agctggtgca actcgatcac gcaacggaag ctgtctatgc
     1501 cttggagttc gaggaaccgg cgctgattac gctggggctc tgttatgaag aacggcctcg
     1561 gttcagcggc ggcgcctatc atccggtctt aaaacgagtg gacgaatttc tacagaaacc
     1621 gttgcatgcc gctctgagca ttcgtcagca gcacgccaag accgtcctgg cgctggacga
     1681 tcttatcgtc aaacaagtcg aaaccatcaa ggccaagggg ttgaccagtc catatctcaa
     1741 aagcttcgtc gtggcccgcg tgaaccccat tcggttccgt cccaaagatg caccgccctt
     1801 gtcgttcgat gacgcactcg atcgcatgac gcaggccgcg gagaagttca atccagacaa
     1861 aatcaagatg gacgatctgg ccaaatccgg cggggttccg gacgaaaacg agtagcccgt
     1921 tcctacctta gatgccggca ttcgttcgcc actgtcttgc ccctgcatag ctagattcac
     1981 ttaggcctct gggctatact gccgccggat tcgaggaatg tctcagcaat tcactggagt
     2041 ccgcggacct catcatggcg atcaagcttc acgcgacgtg gcttctcttg ggagcgctga
     2101 ttgcccctct ccccttgtgg gccgatgcca atctggatta ccaaaatcgt ggcgaccgct
     2161 tcgagggagt caaactgaaa ccggtttccg gctacgacat caaagtcatt tccgtccttg
     2221 cagattatcg cgagcctgct gaacgtctgc ctgaccagct ccgagtcgga ttctacttgc
     2281 agagccagac ccaggtggtc gggattgaag gcggagatca aaacggcctc gcggccctgc
     2341 gtgtggtcgg gccgattcca gacggcgtgg tggcattgcc gctggatttg atgacaaacg
     2401 ttgtcggcgg tgaggcgctc acattcatcg gctacgtacc aagaggcccg atggaatccc
     2461 tcgccgccgg aggaccagac acgcaaggtg cttcggggca gtcttcaggc gctcggcatt
     2521 cgtgacgctg tcggccacct cgaatatcag gtgcgcatgt atgcaggcgc ctatgtatga
     2581 tggagcgcct gggagaggtt ttcgctgcat ggaagatgtc agaaaagaca atgagtttga
     2641 gttgatttct tccgtcttgc ccttgctctc aaagcccctt tacaatgttt tcttgttatg
     2701 catgaaagga gatttccatg agtcttgctg acaataaatg cattccctgt cgtggcgggg
     2761 taccgccgtt gccgaacgat cgcactcagg cactattgaa ggaactaggc cgaggctggt
     2821 ccctgaacgg acaaggacat cttgagcggt tgtatacgtt caaggatttc gcgcaggcgc
     2881 tcgagttcgt gaacaaggtc ggtggggttg cagaggccga gggtcatcat cctgatctgt
     2941 atctggcctg gggaaaaacc aaggttgaaa tctggacaca taagatcaat ggcttgaccg
     3001 agagcgactt ctacatggcg gccaaggccg accgggaatt tgagccgttc agggcaggta
     3061 cctagcattg ggccctcact tgcattcctg atatagtccg ctcgcccttt cgtgctgagt
     3121 ttgtcgaagc atgagtcttt cggcaacctc aggacagggg gtcggtctgg tgggatcgat
     3181 gcatccgcac tgacgacgaa atccgtagtc gtggtctgaa ccgagtcatc gatcaagtcg
     3241 acatgggcca agcaagaatg aggcgacgga agggaatgcg gcgtggagtg ttgattgcgg
     3301 gtcttgtata ggtcatcggt atgccttcga aatctgaggg gcaacaacct tctctcgtgc
     3361 atggccgaca gccacagtcc acaggcgcca ttactgaccg gcagcagtcg ctggataacg
     3421 cagacgagcc gaaagccccc cgtaggccgt ttgaacagga gtcgtccacc gagacgtccc
     3481 gctccgaatt gccggacgcg ccggtaggat ccttctctcc tgagagcaca acagaaggtc
     3541 cagacgcaga tccaccatcg gaatcagatg aggaccagcc tggtgcatcg actcgtccag
     3601 cacgatcaaa gcagtctcca ccatacctca ttatcggagc ggccttgctt gtggtcatgg
     3661 gagcatttgt ggggcaatgg atcttgtcac ctaccctgtc ttctattctc acgaagaatc
     3721 cgtcagcatt tcagacaatg gaatcgcgac gtagccaaag ccaggggatg acgccggacc
     3781 ctgcgcccgc cccgatagtc ctggtgccgg ctggggagtt catgatgggg gcgcgccagg
     3841 aatacggaat ggccgacaaa gatgagcgga cgatccatac ggtgtatctg gatgccttct
     3901 acatcgacca atatgagatc accaccgctc gctatgccaa attctttcaa gagacgaaac
     3961 gatccgcacc aaagtattgg tctgaacagg tcctgaagca gcatgaacgc aagccagtgg
     4021 tgggggtgga ctggaatgaa gctgtcgcct actgctcttg ggcaggaaaa cgcttgccca
     4081 ccgaagccga atgggagaaa gcggcgcgag ggaccgacca acgtctgtat ccatggggga
     4141 atgaagaacc cggtcgagaa cgggcaaatt tcgaccgctg ttgtgattct agccattatg
     4201 ggatcctgac cgacgtcgga tcctttgatg gagggaaaag tccctacggt gtctatgata
     4261 tggccggcaa tgtgtgggaa tgggtggcgg attggtccga cggaggttat gacgccacga
     4321 gcctggagcg cgatccgaca gggccttcga ggggtgaaaa acgtgtggtc cgaggcgggt
     4381 cctgggactc ggctccgacc tacgttcgat cctcgtaccg gctcaggctc tcgcccacgt
     4441 ttcggctcga caatatcggg tttcgttgtg cccgggacgt t
//
LOCUS       DATNDD010000150         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_618687, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000150 DATNDD010000000
VERSION     DATNDD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618687"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VN647_03595"
     CDS             <1..450
                     /locus_tag="VN647_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HWG96147.1"
                     /translation="CVPVLIHDPKRHVVAAVHAGWRGAVAGIVSKTLTLLESRFGSCM
                     EHVRISIGPSAGRCCYEVDEPVLDQLCQRFPDWNKVVRTRGDGKADLDLKALVKEQSQ
                     ALGVTPKSITTVNLCTICHEDLFFSYRRERKVNGTMVSAIGLPMRRE"
     gene            533..1243
                     /locus_tag="VN647_03600"
     CDS             533..1243
                     /locus_tag="VN647_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWG96148.1"
                     /translation="MESLIQEAIGKRVQSVLTKIRSAEEKAGRPAGTVRLVAATKTVT
                     VEHIAEGVRAGLFILGENRVQEALPKIAAFTQASVHWHFIGQLQRRKVRSVIGLFDLI
                     HSVNSLDLAQEIDRRAGEAGRRQNVLLEVNIGKEPTKAGFHPDDVVRSVPMMAQLSHV
                     CVKGLMTIPPPTADPDSARPYFRTLRDLARQIAAFDLRTVRMDELSMGMSNDYEVAIE
                     EGATLVRVGTAIFGARDV"
     gene            1236..2054
                     /gene="proC"
                     /locus_tag="VN647_03605"
     CDS             1236..2054
                     /gene="proC"
                     /locus_tag="VN647_03605"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWG96149.1"
                     /translation="MSSSTHSQSISFVGGGRMAEALISGVLSAREYKADQIHVADPDT
                     VRLDHLKRQYGIQIALTNHEAVALGDIVVLAVKPQVTAEVLKEIGDVLTKRLVISVVA
                     GVRLGRIIEACGPQARVIRAMPNMPAMVGEGMTALAIGPGVGDNGIACARQIFESVGR
                     VVPIDERFMDAVTGLSGSGPAYVFLMIEAMADGGVKMGLPRETSSLLAAQTVLGAARM
                     VLETGQHPASLKDQVASPGGTTIAGLHRLEQGGLRGLLIDAIESATKRSRELGG"
     gene            2054..2350
                     /locus_tag="VN647_03610"
     CDS             2054..2350
                     /locus_tag="VN647_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWG96150.1"
                     /translation="MFVVGNTLLGFATVLDYVLTFYTWIVIARALISWVNPDPWNPIV
                     QFLSRATEPVLAPIRRRLGWSMGVDLSPLVVIVAIWFLQIAVVQSIKDIAVRMN"
BASE COUNT          496 a          574 c          751 g          543 t
ORIGIN      
        1 tgcgttcccg tactgataca tgaccctaaa cggcatgtcg tggccgctgt ccatgccggc
       61 tggcgaggag ctgttgccgg cattgtctcc aaaacgctga cactgttgga gtctcgcttt
      121 ggctcgtgta tggagcatgt acggatcagt atcggtccat ccgctgggcg ttgctgctat
      181 gaagtggatg agccggtcct cgaccagctg tgtcagcgat ttccggattg gaacaaagtg
      241 gtacggacga ggggagacgg aaaagcggat cttgatttga aagcgctcgt caaagaacag
      301 tcgcaggctc tcggggtgac tccgaagtcc atcacgaccg tcaatctctg tacgatttgc
      361 catgaagatc tctttttctc ctatcgtcgg gaaaggaaag tcaacggcac catggtcagc
      421 gccattgggt tgccgatgag gcgagaataa gtcagactct gccaattctg aggctcttcc
      481 gctagaataa ggacggttcg ctgaatggcg gcagcggctc gcgtggagga tgatggaatc
      541 gctcatccag gaagcgatag ggaaacgtgt tcagtcggtt ctcacgaaaa ttcggtcggc
      601 ggaagaaaag gccggacgtc ctgccggtac agtgcgattg gtggccgcga cgaagactgt
      661 caccgtcgag catattgcgg aaggtgtgcg tgccggtttg ttcatattgg gtgaaaatcg
      721 tgtacaggaa gcgcttccta aaatagccgc cttcacgcag gcatcggttc attggcattt
      781 tatcggacag ctgcaacgaa ggaaggtacg atccgtgatc ggcctcttcg acctgattca
      841 ttcggtgaat agcctagacc tggcgcaaga gatcgatcgc cgtgcgggag aggccggccg
      901 tcggcaaaat gtcttgctgg aagtgaatat cgggaaagaa ccgacaaagg caggatttca
      961 tcccgatgac gttgttcgat cggtaccaat gatggcgcaa ctctctcatg tctgtgtcaa
     1021 aggcctcatg acaattcccc cgccgaccgc tgatccggat tcggccaggc cgtatttccg
     1081 cacactccgt gatcttgccc ggcagattgc ggcattcgac ttacggaccg taaggatgga
     1141 tgaactgtcg atgggaatgt cgaacgatta tgaggtcgcc attgaggaag gggcgacact
     1201 ggtccgtgtc ggcaccgcca tctttggagc gcgtgatgtc tagttcaacg cactcacaaa
     1261 gtatctcctt tgtgggtggt ggccggatgg cggaggcatt gatcagtggg gtgctttccg
     1321 caagagagta caaggccgac cagatccacg tagctgatcc agatacggtc agactggacc
     1381 atcttaagag acagtacggc attcagattg ctctcacaaa tcatgaggcg gtcgccttgg
     1441 gggatatcgt cgtcctggcc gtgaaacctc aggtgacggc cgaagtcctc aaagagatcg
     1501 gggatgtgct gacgaaacgg ctcgtgatct cggtggtcgc gggagtgcga ctcggtcgaa
     1561 ttattgaggc ttgcgggccg caggctcgcg tcattcgtgc gatgccgaat atgccggcaa
     1621 tggtcggcga agggatgact gctctggcga tcgggcctgg ggtaggggac aatgggatag
     1681 catgtgcacg gcagatcttt gaatcggtcg gccgggttgt gcccatcgac gagcgtttca
     1741 tggatgccgt gaccgggttg agcggaagcg gaccggccta cgtctttctc atgattgaag
     1801 cgatggccga tggcggcgtc aagatgggtt tgccgaggga aacgtcaagt cttctcgccg
     1861 cgcagaccgt gttgggtgcg gcacggatgg tcttagaaac ggggcagcat cctgcgagtc
     1921 tcaaggatca ggtggcgtcc cccggaggaa cgacaattgc cgggttgcat cggcttgagc
     1981 agggaggtct ccgaggtctg ttgatcgacg ctattgagtc tgcgacgaag cgttctcggg
     2041 agctcggagg ctgatgtttg tggtaggaaa taccttgtta gggttcgcca cggtactgga
     2101 ctatgtattg acgttctata cctggatcgt catcgctcgg gccctgattt cttgggtgaa
     2161 tccggatcca tggaatccga tcgttcaatt cctcagtcgt gccaccgaac cggtcttggc
     2221 tccgatcaga cggcgattag gctggagcat gggcgtcgat ttgtcgccgt tggtcgtcat
     2281 cgtggccatc tggtttttac agattgcggt cgtacagtcg attaaggaca tcgcggtacg
     2341 gatgaattga ttcaaccaac gggg
//
LOCUS       DATNDD010000151         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1691337, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000151 DATNDD010000000
VERSION     DATNDD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691337"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1083)
                     /gene="trpD"
                     /locus_tag="VN647_03615"
     CDS             complement(43..1083)
                     /gene="trpD"
                     /locus_tag="VN647_03615"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWG96151.1"
                     /translation="MPIQDLLAKIAKGPKASKDLTWDECKQAMKALIEGEAMPAQVGA
                     FLIAMRFKTESVTELAALTAAARQYVLPLTVSRDLALVDVPTYAGKQDTFHATIAASI
                     VAVAAGAAVLMHGYDGIPGRPGSAGVLRAMGIPVDADPKLVSEDVNRKGFAYLDIGLY
                     HPPLYRFLEMRQELGVRNVFHPIARLLNPARASVQVVGLTHPPHFEKTAEVLRILGCR
                     RALVIRGVEGDPELSASMATRVLELRDERITPLGVAPKDFGLAFVPSREMAGFPPDQR
                     DREADLIRRILRNQVQGGPRDWVLMNAAMLLYAAGKGMSLSACFPAVRAALDGGAAGR
                     KLDELVRQPVAV"
     gene            complement(1085..1801)
                     /locus_tag="VN647_03620"
     CDS             complement(1085..1801)
                     /locus_tag="VN647_03620"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWG96152.1"
                     /translation="MAVSDQSKLIGELKEWGASFETKQPQDILTAAIERYGPKIVVAC
                     SFGAEDVVLVDMVHRINASVPLFYLDTDFLFPETYATRDRVIERYDLQPTQVIQVKSL
                     LTPEVQAESYGEALWARNPDQCCQLRKVEPLTRVLRGYDAWITGIRRDQAPSRADAGF
                     IEWDQKFGLIKVNPLARWTWTDVWTYIKVYEVPYNPLHEQNYPSVGCMYCTAPVAPGD
                     DPRAGRWKNFAKTECGLHKS"
     gene            complement(1817..>2622)
                     /locus_tag="VN647_03625"
     CDS             complement(1817..>2622)
                     /locus_tag="VN647_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWG96153.1"
                     /translation="LIPYTIVPSFQEDPTFYYDWEGEEEFILEDLGPGECAGGALEMI
                     ENGILEADQELYQAKLLVEKHQYAVSVNKSYRAVLAAAKAMLVTEGLEPSTDSETFIE
                     FDSRVAQKDVVPSVYRDLSKKVGDLGPKETSSESAREKMAFAKGFVDACRAATDQMGK
                     DLKLSAMKEEMPPVVAAPVETKPVAPAPTGAPVYDLRGVACPMNYVKTKLKLEMMDAG
                     EQLEVWLDAGEPIKNVPMSLKNDGHKVLVQEALEPEASHYRILVEKVEG"
BASE COUNT          567 a          816 c          653 g          586 t
ORIGIN      
        1 ttgccgcagg tgcttcatat ccggaatcct tccactttcg tcctacactg cgaccggctg
       61 tcgcaccaat tcatcgagct tgcgccctgc ggctcctccg tcgagcgccg cgcgtacagc
      121 cgggaagcag gccgacaggg acatgccttt gcccgccgca tagagcaaca tggctgcatt
      181 catcaacacc caatctcgcg gcccaccctg aacctggttc cgaagaattc gccggatcag
      241 atcggcttcc ctgtcccgct gatcgggagg aaacccggcc atctctcttg atgggacaaa
      301 tgccagcccg aaatctttcg gagccactcc cagcggcgtg atgcgctcat cacgcagttc
      361 cagaactctc gtcgccatcg aggcggacaa ctccggatca ccttcaactc cacggatcac
      421 caatgctcgt cggcacccga ggatccgcaa gacctcggct gtcttttcaa aatgcggcgg
      481 atgcgtcaac cccacgactt gcacagatgc ccgagccggg ttcagaagcc tggcgatcgg
      541 atggaacaca ttcctgacgc cgagctcttg acgcatctcc agaaatcgat agaggggtgg
      601 atggtacagt ccgatatcca ggtaggcaaa acctttcctg ttcacgtctt cggaaaccag
      661 tttgggatcg gcatcaaccg gaatgcccat ggctctcagc actccggcac tgcccggtcg
      721 gccaggaatg ccgtcatagc cgtgcatcaa gactgcagcg ccggctgcaa ctgcaacaat
      781 agacgcggca atagtcgcgt gaaacgtgtc ctgcttcccg gcataagtcg ggacatccac
      841 cagagccaag tcacgcgaga cagtgagggg aagcacatat tgccgagccg cagccgtcag
      901 cgccgccaat tcggtaaccg attccgtctt gaatcgcatg gcaatgagaa aagcccccac
      961 ttgcgccggc atggcctcac cttcaatcaa tgccttcatc gcctgtttac actcatccca
     1021 tgtaagatcc ttggaagctt tcggaccctt ggcaatcttg gcgagaagat cctgaatcgg
     1081 catgttacga cttgtgtagc ccgcattcgg tcttggcaaa attcttccag cgaccggccc
     1141 gcggatcatc gccgggtgcc acgggagcgg tgcagtacat acagccgacg ctgggataat
     1201 tctgctcgtg cagggggttg tagggaactt cgtagacttt gatgtaggtc cacacatcgg
     1261 tccacgtcca tcgggccagg gggttcacct tgatcaatcc aaatttctga tcccactcga
     1321 taaacccggc atccgcacga gacggggctt gatcacgcct gattccggta atccatgcgt
     1381 cgtatccccg cagaacgcgc gtcaatggtt cgactttccg cagttggcag cactggtccg
     1441 gatttcgagc ccacagcgcc tcgccgtacg attctgcttg gacctctggt gtgagcaatg
     1501 acttcacctg gatgacctgc gtcggctgaa gatcgtatcg ttcaatcact cgatcccgcg
     1561 tggcataggt ttcaggaaac aaaaaatcgg tatccagata gaacaacggt actgaggcat
     1621 tgatccgatg caccatatcg acgaggacca cgtcttccgc tccgaagctg caagccacaa
     1681 caatctttgg gccgtatcgc tcgatcgcag ccgtcaatat atcttgtggc tgcttcgtct
     1741 cgaatgaggc accccattcc ttaagctctc cgatgagttt ggattggtcg cttaccgcca
     1801 tggcgaatca gatcccttac ccttcgactt tctcgaccag aatccgataa tgcgaggctt
     1861 ccggctccaa ggcctcttgg accagcacct tgtgtccgtc attcttaaga ctcatcggta
     1921 cattcttgat cggttcaccc gcatcgagcc acacttccaa ctgttcaccg gcgtccatca
     1981 tttcaagttt caacttcgtc ttcacatagt tcattgggca tgccacgcca cgcaagtcat
     2041 aaacaggcgc acccgtcgga gcaggagcaa cgggtttggt ttcaaccggt gcagcgacga
     2101 ctggaggcat ttcttctttc atagccgata gcttcagatc cttccccatt tgatccgtcg
     2161 ccgcacggca ggcatcaaca aaacccttgg caaatgccat cttctccctg gctgactcgg
     2221 acgacgtctc tttcggcccc agatcgccga ccttcttgct gaggtctcga tagacggacg
     2281 gcacaacatc tttctgtgca accctgctgt cgaactcgat gaacgtttcg gagtccgtcg
     2341 agggttccag tccttctgtc accagcattg ccttggcggc ggccagtacg gctcgatagg
     2401 atttattcac cgacacggca tactgatgct tctcaacgag cagcttcgct tggtagagct
     2461 cttgatcggc ctccagaatg ccgttttcaa tcatctcgag cgcaccaccg gcacattcgc
     2521 cggggccgag atcctcaagg ataaactctt cttctccctc ccaatcataa tagaacgtgg
     2581 ggtcctcttg aaaagacggc acgatggtat acgggatcaa ct
//
LOCUS       DATNDD010000152         4350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2909003, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000152 DATNDD010000000
VERSION     DATNDD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909003"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..432)
                     /locus_tag="VN647_03630"
     CDS             complement(100..432)
                     /locus_tag="VN647_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96154.1"
                     /translation="MSPGSMRRDFPGKNNTGTTRRSAASACCWMAGRSHRVFANAAKE
                     ATLLIVFNAYHEGVDFFLPECEGGREWSLLVDTNAPEHHDKNAIPVGSSYVVTGCSLV
                     LFKLLLES"
     gene            2429..2983
                     /locus_tag="VN647_03635"
     CDS             2429..2983
                     /locus_tag="VN647_03635"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWG96155.1"
                     /translation="MTRTLCSITTVIVFAAFPIMSVSAASPGDRGDLFLKMVAAGNQA
                     EIALGQLALQQAESAEVKQFGAKMIQDHQKASHEIQQVASKEEVQLPRQMSEKQRLKQ
                     VELAQLSGKAFDLAYMQYMLKDHRKEVKQFEETAQQLQNRVVKTWAFNTLPVLKQHLE
                     LASNIAARLGPMNVYNEETAPPSK"
     gene            3446..>4350
                     /locus_tag="VN647_03640"
     CDS             3446..>4350
                     /locus_tag="VN647_03640"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWG96156.1"
                     /translation="MVDSKKPSAAAAQNETDMRLARDPKKGTLSRLGGRWIIPVAIVG
                     VLTLTVLLASALLSEPLKNYAKREASERLPHFDITIGTLHLQPFRLGMGVEDLQVRLR
                     AQTDPPLAEIPQVKAHLRFLPLFAGKIDVNLQIEGPHVAATSQQVGSILHTPKKEAVK
                     LEAIAWQDTVRNMIPIHVSLSLSNGEVRYQSDPHADPIQLDELQITAANVTNRPPEHE
                     AYPSELRVKAHLADNAEVALNSHIDLLATPSPRMDGDLKVRHLTLPTLRPLMGQYNIQ
                     LRQGVFDMTGHVNYASPTTVVDIHH"
BASE COUNT         1047 a         1193 c         1117 g          993 t
ORIGIN      
        1 tctgcccgtt cacgttcggc cgtccagtgc gcctccgcaa gccagcgcgg tcaggactgc
       61 ggggagatcc gcaaggcgat aggctcaccc cctggtccct caggattcca ggagcaattt
      121 gaacagcacc aacgaacaac cggtcaccac atacgagctt cctactggaa tcgcattctt
      181 atcgtgatgc tcgggcgcat ttgtgtcgac taataacgac cattcccgtc cgccttcaca
      241 ttccggaagg aaaaagtcga cgccttcatg atacgcatta aaaacaatga ggagggttgc
      301 ttccttagcc gcgttggcga atacccgatg actgcgcccg gccatccagc agcatgccga
      361 agcagcggag cgacgtgtcg tgccagtgtt gttcttcccg gggaagtccc gtcgcatcga
      421 tccaggtgac atcccgaact cctagttcct cgatatagtg gccgtttaag aataaattac
      481 gccgcaatat gggatatttg tgtcgaagcg tgggaggatc tgaacgaacc gtaggagagc
      541 tgactcggct tcaaatggtg ggatcctcag ttcgtccttc tgcaccacaa ttccatgacc
      601 gattgttgaa gctcccatcc gcgttctgtt caccattcgc ctcattgtgt ttatcattgt
      661 aggagacgac atcgttgagc gtaaacccgt gtgggcagtg ataaagttca cgcaggccca
      721 gggtcttcga ccttgatgat gaaagagtga ggcagaagcg ctcagccgat cggctaacaa
      781 ggacaccggt aattcccccc gccaaaagtc ccgcacctga tcccgatatt tatcattcca
      841 ctcggcccag cccggaggaa agccaccgac ctggtagccg ccgggcccgc aatcccacgg
      901 ctcagcaata agcttgacgg ttcccaggac cgggtcctga ctacaggcct tgagaaagcc
      961 actttgatta tgaaactcat tcggctcacc tgacatccag acataatttc ttcctagcat
     1021 attgaccttc aacgcagctt cctactagga aaacgcatag tcggtttatc ggtttaatgg
     1081 tattaaaaag cacaagacaa taacagcacg atagtcgaca agaaaaaaga gacgaatcaa
     1141 acatagccga tggtaaggtt ggcaccgatc cagaatgaaa tagtccacac tctttgattg
     1201 agtgtctacg atcgcgccgg ggggatggga tcgttgccgc gcggcattct gctttgacga
     1261 agagaggctg accgccatac tttccttggc tcgtcaattc ggatatgcca gtgtccgggc
     1321 agtgagtggg gccgggggct atttatatct gaagaggtgg gccaggccct tatgggttat
     1381 gagcaccaag ccacggcccc ttcatcgatg aaactctatg catcatgctc tgttgctatg
     1441 ccaaacgcca atgtttggca accgacatgt tcgatatcat gagccccttc ctcgggcagt
     1501 gctttggact cacgctggtt gagccagtat ctgaactgag gcaaccattt gtcaccgtac
     1561 ctgtgcttga tcttcatatc gtcctctctt tcctttctgc aaccattgac aaggctgaac
     1621 gccctggctc tcgcaccatg tgtccttgtt gagtgaattc cttggccgaa cgcccagcat
     1681 ccttcaccgc gtcctgtccc tgttgtgcaa attcccctgg ctgatcgtcc ggcattccgg
     1741 acaacctctt ctcccttgcc gcgccgctct gtgccctgtt aggcgaaaat agtaggggaa
     1801 taccggcccc aatcaatgcg cccgccataa atgcggacac atcccatggc ttcccccccg
     1861 ctccctcagc ataatggttt ccgttatacc tcttatcatc ctccttgtgc gtctaaaatt
     1921 agattagaag gcttacactt gagttggatt ttgggtttga ccgtcatgga ccattaaccc
     1981 tacttaataa atctagcgca gtctgagcga gcgccatacg gggagaagac ataggacgaa
     2041 cggtcgcaat gccggatggt ccacccagga taatgataca atcccatcct cacgtattgc
     2101 tcagcgatct cgcgggatct attgaagaat aataaccatt ttgatcgtgt ctgaacgtga
     2161 ggcgcatcat tgtctcgtct ctctaagtcc tctctagcta actctatttc gagatgtttt
     2221 ccccaattac agtttcgatt gtcctatgcg gaagcaatag ctcctaattg tttgaactct
     2281 cctcatcggt aattcctaag gcggaagcga gcctttcacg gttaatcatc acaacgcccc
     2341 acataggacc tttgctagtg gtgcgcttcg gggcggtgta gtgagataaa cgaacaacgg
     2401 ttgattgacc ctcacaaaag gagaccgtat gactcgtacc ctatgttcca tcacgacagt
     2461 gatcgtcttc gccgcattcc caatcatgtc agtatcagcc gcttccccag gcgacagggg
     2521 ggatttattt ctgaaaatgg tggcggcggg gaatcaggcg gaaattgcgc ttggacagtt
     2581 ggcgctccag caggcggaaa gcgccgaggt gaaacagttt ggtgccaaaa tgatccaaga
     2641 tcaccagaaa gcgagccatg agattcagca ggttgcgtcg aaggaagagg ttcaacttcc
     2701 caggcagatg agtgagaagc agagactaaa acaggtagag ttggcgcagc tatcgggcaa
     2761 ggccttcgac ctggcgtaca tgcaatacat gctgaaagac cacagaaaag aggtgaagca
     2821 gttcgaagag actgcccaac aactgcaaaa tcgggttgtc aaaacatggg cgttcaacac
     2881 gttgccggtc ttaaagcaac atctcgagct ggcttcgaac atcgccgcga ggctaggtcc
     2941 gatgaatgtc tataacgagg agactgcccc accctctaaa taaaacgtca ttctggatgg
     3001 ctgggggcat ttcgcgattc tatgcatgcg gagttttaac ttttaaagta tggaggggga
     3061 tcgatggctg aacccatgac gaaattgaaa cacacgagcc atagggaggg agctcaggaa
     3121 cacaggcagg gcaacgtggc ctatagtatt gaagcgcaga cagcgaagat gtcttctgac
     3181 gtgtggttgt gggccgcctt tgccgcgatt ggggtttcca tgttgttcca cggaacggga
     3241 agagagaaga cggccatttg gtcggacagt gggtcgcgcc cttcttgctg ttcggtgtgt
     3301 acaataagtt ggtcaaaatc ggcgtgtctt atcgcacttc ttaagtccgt gacatcgagc
     3361 caatcacgct tagggcatca atacgggctg ctcacgtatg gcaatctgat ggcgcatcag
     3421 attttaaccc actgaaagag cccagatggt cgattccaaa aaaccctccg ccgctgccgc
     3481 gcagaatgag acggacatgc gcctcgctcg agatccgaag aagggtacac tctcacgatt
     3541 gggtggccgc tggatcatcc cagtggcgat cgtcggtgtc ctgactctga cggtgcttct
     3601 tgccagcgcc ctgctcagcg aaccattaaa gaactatgcc aagcgcgagg cgagcgagcg
     3661 actgccccac tttgacatca cgattggcac actccactta cagccgttcc ggctgggaat
     3721 gggtgtggaa gacctccagg tgcgtcttcg agcccaaacg gatccgccat tggcagagat
     3781 cccgcaggtg aaggcccacc tccgattcct ccccctgttc gccggcaaga ttgatgtcaa
     3841 tctacagatc gaaggcccac acgtagccgc aacgagccag caagtcgggt cgatcctcca
     3901 tacccccaag aaggaagcag tcaaactgga ggcgatagcc tggcaggaca ccgtgcggaa
     3961 catgatcccc attcacgtgt cgctgtccct ctccaacggt gaagttcgct atcagagtga
     4021 cccacatgcc gaccccatcc aactcgatga acttcagatt accgcggcga atgtcaccaa
     4081 tcggccacct gagcatgaag cctatccgtc ggagcttcgt gtgaaggctc atttggcaga
     4141 taatgccgaa gtggccttaa atagccacat cgatttgctc gccacaccaa gcccacggat
     4201 ggatggggac ctgaaagtcc ggcatttgac gcttcccacc ttgcggccgc tgatggggca
     4261 atacaatatc cagcttcgtc aaggggtttt cgatatgacg ggtcatgtga attatgcctc
     4321 tccgaccacg gttgtcgata tccatcattt
//
LOCUS       DATNDD010000153         5482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2530839, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000153 DATNDD010000000
VERSION     DATNDD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530839"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..614
                     /locus_tag="VN647_03645"
     CDS             72..614
                     /locus_tag="VN647_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96157.1"
                     /translation="MQFAEAEQVARSRPLPMVDAAWEIFRKTLPDELKAKLQQKKLGL
                     DYRTLDRAVAKGGDIKYIDFRKDWSPHFKRLCIMPDGRLVETGGVQDFARLHGITVAQ
                     AQGLIEQGGALEVNGEVLACQIVNGQPAVARFSAKNYAKAKDLVSSKGLHLMDALSEV
                     GYNDPAMMRGLARKELAGKR"
     gene            complement(647..1012)
                     /locus_tag="VN647_03650"
     CDS             complement(647..1012)
                     /locus_tag="VN647_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96158.1"
                     /translation="MATILVIDDEQSIRGLLKEVLVKAGHRVLEAEDGRKGLTLYQKE
                     PVDLVIMDLLMPETDGLEATLQLTREYLDAKVIAITGAQGDHNFLDVAKLFGARRAFE
                     KPFDINKLLDAVKEELAAA"
     gene            1371..1943
                     /locus_tag="VN647_03655"
     CDS             1371..1943
                     /locus_tag="VN647_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HWG96159.1"
                     /translation="MSTTDQPKWAWTRRSFLQVSLVGTLLLSGRLAGPQPVQARELPE
                     GRLTLVNVWTNERLDVTYRDEAGNYDLAALDDVNYLLRCHYTGEVGAIDVRVLEHVNL
                     VQKKLGSLEEIHVISGFRSPEYNAMLVRTDRRVAKNSLHMQGQAIDLLIPGISTTKLR
                     QAALELRYGGVGFYKRSSYVHLDSGPFRHW"
     gene            complement(2042..4264)
                     /gene="glgB"
                     /locus_tag="VN647_03660"
     CDS             complement(2042..4264)
                     /gene="glgB"
                     /locus_tag="VN647_03660"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWG96160.1"
                     /translation="MSLELTIQRDDLDRLIAGTHWSPHSILGPHAITIDGRPFLAIRT
                     WLPRVKDVEVVSNPSLWRMTRVREEGLYEVLLPGETQALSYRLRITHHGGTVTELHDP
                     YAFPPLLTDFELHLFAEGTLYKAYDNFGAHVRTVEGVCGVHFVVWAPNAVRVSVVGDF
                     NGWNGLCHPLTSRGATGVWELFIPDLPEGTLYKYEIRSREHTAPLLKADPYAVASELR
                     PRTASVVHDLSTYTWHDGEWMAARAEWDPLTASVSIYEVHLGSWMRVPEENNRWLTYE
                     ELAAKLIPYATDLGYTHLELMSVTEHPFDGSWGYQSTGYFAATSRYGDPKGFMAFVDA
                     AHQAGLGVIMDWAPAHFPDDSHGLALFDGTHLYDHADPRLGYHPDWHSRIFNYDRVEV
                     RTFLLNSALFWLDKYHIDGLRVDAVASMLYLDYGRRSGEWIPNEFGGKENLGAVSLLK
                     ELNVLIHREFPGAVTIAEESTAWPGVSRPTYTGGLGFTFKWNMGWMHDMLAFFQHDPI
                     YRRFHQNQITFGLLYAFSENFILALSHDEVVHGKRTLLDKMPGDVWQRFSNLRLLYGH
                     MYSHPGKKMLFMGGEFGQWREWNHDGSLDWHLCEEEPHRGLQRLIRDLNRVYREEPAL
                     HEVDFDWSGFQWIDFSDADNSVIAYLRKAKSTQTAIVCLCNFTPVPRLGYRIGVPEPG
                     WYHELINTDGIAYGGGNMGNGRGVHTAELPSHGFRYSLTVTLPPLSILWLKQPPTNNL
                     "
     gene            4432..>5482
                     /locus_tag="VN647_03665"
     CDS             4432..>5482
                     /locus_tag="VN647_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HWG96161.1"
                     /translation="MSSLMLALLIFGLCYVLIMTERLHKTIVALFGAAVMIGLGVVSQ
                     EEAFYSHEFGVDYNVVFLLIGMMVIINIVRETGLFEVLAVWAAQRADAKPFRLLVLLA
                     ILTAVLSAMLDNVTTVLLMAPVTLAITKRLELNPVTFLMTEALASNIGGTATLVGDPP
                     NIMIASKAELSYLDFLVVMGPIVIVIMAVFLTALWFIFGRTMTVAPHLREAVLALSSR
                     EFVSDDAFLRRCLFLLAVVNVGFCVHSLVHLEPATIALLGASLFMVIGHARRKPEDTE
                     ELTYLAEVEWKTIFFFIGLFILVGGLVKVGVIRYLADQLVTVTRGNLTGSTMAVLWGS
                     AILSAVVDNIPYVAAM"
BASE COUNT         1203 a         1404 c         1581 g         1294 t
ORIGIN      
        1 ctcgatgatt atttgcctgc tggatcgatc cagtcgggca agggccgaaa cagacctgga
       61 cgctggacct gatgcagttt gccgaggcgg agcaagtggc tcgatccagg ccgcttccga
      121 tggtcgatgc ggcatgggag atctttcgaa agacgctgcc ggatgaattg aaagccaagc
      181 tgcagcaaaa aaaattagga ttggattacc gaacgttgga tcgcgccgtc gccaaaggcg
      241 gcgacatcaa gtatatcgat ttccgaaagg actggtcccc acatttcaag cggctctgta
      301 tcatgccgga tggccggctt gtcgagaccg gcggggtaca agattttgcg agactccacg
      361 gcatcaccgt ggctcaggcc caagggctga tcgaacaagg tggtgcgctc gaagtgaacg
      421 gcgaggtgtt agcctgtcaa atcgtgaacg gtcagccggc tgtggcgcgg ttcagtgcaa
      481 agaattatgc caaggccaag gacttggtat cctcgaaggg cctgcatttg atggatgccc
      541 tcagtgaagt gggatacaac gatccggcca tgatgcgagg tctggcgcgg aaggaattag
      601 ccggcaaacg ataattccat gaaatatatg acgcgattac tcgagttcac gcggcggcga
      661 gttcttcctt caccgcgtca aggagcttat tgatgtcgaa cggcttttca aaggcgcgac
      721 gagcaccgaa aagcttagcg acatccagga agttatggtc gccttgcgcc cctgtgattg
      781 cgatcacttt ggcgtcgaga tattcgcggg tgagttgaag cgttgcctca aggccgtccg
      841 tttccggcat caacaagtcc atgatcacga ggtcgacagg ctctttttgg taaagtgtga
      901 gccctttacg accgtcctct gcttcaagca cacggtgccc tgccttgaca aggacctctt
      961 tcagcagccc tcggatagat tgttcgtcgt caataacgag aatggtggcc atacatgctc
     1021 tactgatttt ttctgatctt accctggcgc ccgcgaaaca gtctagaaag aaaatgattt
     1081 ctggaatagg tgcaagaggt gaaatgaaga tgagtccgat tggcctcata cgttgcgaca
     1141 accacaggcc ccctgatgag cagtgaccag gtcacttttt ataaaattcg aatgaaaata
     1201 catacatcgt ttaatgttta tatggatgga tagtcatcca attttttgga tgagaagagt
     1261 ctatccagtg cactagggac aaatcatttg actcaatttt ggattttttg ttacaaggcc
     1321 aagagttctt tttgaatgat caccgttcac ttttttctgg agttgtttca gtgagtacta
     1381 cagatcagcc caaatgggcc tggaccaggc gatcgtttct tcaggtttcc ttggtgggga
     1441 ctctattgct gagtggacgg ttagctggtc cacaacccgt gcaggctcgt gaactgcccg
     1501 agggcaggct gacgctggta aacgtgtgga ccaatgagcg gttggatgtc acctaccgag
     1561 acgaagccgg gaactatgac cttgcggcgc tcgatgatgt gaattacctc ctgcgctgcc
     1621 attacacggg tgaagttggg gcgatcgacg tccgtgtgct ggagcacgtg aatttggtgc
     1681 agaagaagct cggcagtctg gaagaaattc atgtgatttc cggctttcgg tctcccgagt
     1741 acaatgccat gttagttcgg acagaccgac gagtcgccaa gaacagttta cacatgcaag
     1801 gacaggcgat tgatcttctc atccccggta tttctacgac aaagcttcgc caagccgcgc
     1861 tcgaactgcg gtacggcggg gtcggcttct acaaacgctc gagctatgtg catttggatt
     1921 ccggtccctt ccgacattgg tagccctcgc tcctgctgca ggcgaatctt ttccttcttc
     1981 caacacatca gtttacgaaa aggatcggga ctgtcgttgt gtcgaaacgg ggtagtgggg
     2041 gttataggtt gttggtcggc ggctgtttca accagaggat ggatagggga gggagggtga
     2101 ccgtcaaaga gtagcggaac ccgtggctcg gcagttccgc agtatgaaca cccctgccat
     2161 ttcccatatt gccgccaccg taagcgatcc cgtcggtgtt gatcagttca tgataccaac
     2221 ccggctctgg gacgccgatc cggtaaccga gacggggtac gggcgtgaag ttacacaggc
     2281 agacaatagc cgtttgcgtg ctttttgcct ttcgcaggta ggcaatgacg gaattgtcgg
     2341 cgtcgctgaa gtcgatccac tgaaatccgc tccaatcaaa gtcgacctcg tgcagggcgg
     2401 gttcttcgcg atagacgcgg ttcaagtcgc ggatcagacg ttgcaagcca cgatgcggct
     2461 cctcctcgca gagatgccaa tccaagctgc cgtcatgatt ccattcccgc cactgaccga
     2521 actctccgcc catgaagagc attttcttac ccggatggct gtacatatgt ccatagagta
     2581 gccgaaggtt tgagaaccgc tgccacacat cgccgggcat tttgtccagc aaggttcgtt
     2641 taccatggac cacttcgtca tgtgagaggg cgaggatgaa attctcgctg aaggcataga
     2701 gcagaccgaa cgtaatttgg ttttgatgga accgacggta gatcggatcg tgttggaaaa
     2761 aggccagcat gtcgtgcatc catcccatgt tccatttgaa cgtaaagccg agcccgcccg
     2821 tataggtcgg tcgagacacg ccgggccagg ccgtggattc ttccgcgatc gtgacggccc
     2881 cggggaattc tcggtggatg agcacgttga gttctttcag gagtgagacg gctccaagat
     2941 tttccttgcc gccgaactca ttgggtatcc attcaccgga ccttcgacca tagtcgaggt
     3001 agagcatcga cgctacggcg tctacccgca atccgtcgat gtgatacttg tccaaccaga
     3061 agagtgcgct gttcaacagg aaggtgcgca cctcgactcg gtcgtaattg aagatccggc
     3121 tgtgccaatc ggggtgatag ccgaggcgtg ggtcggcatg gtcatagagg tgtgtgccgt
     3181 cgaacagcgc caatccatgg gaatcgtccg gaaaatgcgc gggagcccag tccatgatca
     3241 cgccgagacc ggcttggtga gcggcgtcca cgaacgccat gaatcccttg ggatctccat
     3301 atcggctggt ggccgcaaag tagcctgtcg attgataccc ccatgatcca tcgaacgggt
     3361 gttcggtcac cgacataagt tcgaggtgtg tgtagcccag atccgttgcg taggggatca
     3421 gcttcgccgc gagttcttca tacgtgagcc aacgattgtt ctcttccggt acccgcatcc
     3481 atgacccgag atgaacttca tagatggaga cggaagcggt cagcggatcc cattctgctc
     3541 gggccgccat ccactcgccg tcatgccagg tgtaggtgga taagtcatgc acgaccgacg
     3601 cagttcgtgg gcgaagctcg ctcgcgacgg catagggatc cgctttcagc aacggagcgg
     3661 tatgttctcg tgatcgaatt tcgtatttgt agagggtacc ttccggtaga tcgggaatga
     3721 agagttccca gaccccggta gctccacgac tcgtcaaggg atggcacaga ccattccatc
     3781 cattgaaatc accgacgacg cttacgcgga cagcattggg agcccacacc acaaaatgaa
     3841 ctccgcagac gccttcgaca gtgcggacat gggcgccgaa attgtcgtag gccttgtaca
     3901 gggttccttc ggcaaagaga tgcagttcaa aatccgtcaa cagcggggga aaagcatagg
     3961 gatcatgaag ttccgtgaca gtgccgccgt ggtgcgtgat tcgcagccga tatgacagcg
     4021 cttgggtttc acccggaaga agcacctcat acagtccttc ctcacgaaca cgagtcatac
     4081 gccagaggct ggggttggat acaacctcta catccttgac ccgaggcagc caggtccgga
     4141 tcgcgaggaa cggacggccg tcgattgtta tggcatgtgg gcccagaatc gaatggggag
     4201 accagtgtgt gcctgcaatg agacgatcga gatcatcccg ttgaatggtc agttcaagag
     4261 acatcacgct atcctaatgg ttacccgctt gattaggaag gagaaaatgt tctcccgtga
     4321 cggcttcgga tgacagacca gaaagacgac tctgattgca ttgtcgtgac cggccccgga
     4381 taatgcgatg gctctcagga cgatccgtct ccgttcggtt caaggacata catgtcatct
     4441 ctgatgctgg cccttcttat cttcggcctg tgctatgtgc tcatcatgac cgagcggctc
     4501 cacaagacca ttgtggcctt gttcggagcg gccgtgatga tcgggctcgg tgtggtctcg
     4561 caagaggagg cgttttattc ccacgagttc ggggtggact acaacgtcgt cttcctgctg
     4621 atcggcatga tggtgatcat caacatcgtg cgggagaccg gactgtttga ggtgttggcc
     4681 gtttgggcgg cacagcgagc cgatgcaaag cccttccgat tactcgtgct gttagccatt
     4741 ttgacagcgg tattgtccgc catgcttgat aacgtcacca ccgttctgct catggccccg
     4801 gtcactcttg cgatcaccaa gcggttagag ttgaaccctg tcacgtttct gatgaccgag
     4861 gcgctcgcct ccaatatcgg cgggaccgcc acgctcgttg gggatccgcc caacatcatg
     4921 attgccagta aggccgagct cagctatctc gatttcctgg tcgtcatggg gccgatcgtc
     4981 atcgtcatca tggcggtatt tttaacggcc ttgtggttca tctttgggcg aacgatgacg
     5041 gtggcacctc atctgcggga ggcagtactg gccctcagtt cgcgggagtt tgtttcggat
     5101 gatgcttttt tgcgccgttg tctgttcttg ctggcggtag tcaatgtggg attttgcgtc
     5161 cattctctcg tccatttgga accggctacg attgcgttgc tgggggccag cctgttcatg
     5221 gtgatcggcc atgcccgacg aaaaccggaa gacaccgagg aattgacgta tctggcggaa
     5281 gtggagtgga agaccatttt ctttttcatc ggcttattta tcctggtggg aggattggtc
     5341 aaagtcggag tgatccggta tctagccgat caactcgtga cggtgacaag agggaatctc
     5401 accggctcca caatggccgt gttgtgggga tcggcgatac tgtctgcggt ggtggacaat
     5461 atcccctatg tcgcagcgat ga
//
LOCUS       DATNDD010000154         4335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3428708, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000154 DATNDD010000000
VERSION     DATNDD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3428708"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="VN647_03670"
     CDS             complement(<1..2007)
                     /locus_tag="VN647_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG96162.1"
                     /translation="MTQTTAESADYPTILVVDDNPSALYVKSRLLSRQDYRIIEADNG
                     ADALTVATAERPALILLDVNLSDISGFDVCQQLKTQPETKFIKILQTSAARITAPDRV
                     KSLEVGADAYLVEPAEEEELIGTVRALLKLAQHERDNQRLIERLTESEMRYRSLIERM
                     PAAMYTIDRHGRITFYNDQAAELWGRRPNLGDSESQFRGSHKLLWPDGTPLPHDKTPM
                     ADAVQDGTALHGQDVILERSDGTRRHVIVHLDPLRNAEGHIVGAVNLFTDITVRKQAE
                     DALRDAERRYRTIFEQAGVGVAQINSRTGRFERVNRKYCEIVERTEAEMLATTFMSIT
                     HPDDLAEDLAGMEHLRCGLTDSIAMEKRYVRKDGSIVWVLLNVAPLRKLGEELSQHIA
                     VVQDITERKHAEERLRESEERFRMLAEAMPHFVWHTDGQGETEFENQRWYDYTGLTHE
                     TTRHGGWFTVQHPDDAPRLADAWKKAVETGGEYDVETRFRRDADGAYRWFRVRGAPVR
                     DAEGRIRSWVGTCTDIHDRKEAELALRESEERYRATFANAAVGIAHIGLDGRWLQFND
                     SLCTITGYFREELLTMTFVDITSPGDLKTEWAQARRMLAGEIETYSMEKRYVRKDGSL
                     IWVNKTVSLLRDACHRPLHFICIIEDIDDRKQAEEALREAQARLY"
     gene            complement(2004..3890)
                     /locus_tag="VN647_03675"
     CDS             complement(2004..3890)
                     /locus_tag="VN647_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWG96163.1"
                     /translation="MHEGKAADPPPEAFITDSEPFGYQETISIEKQETPKPLPSERDG
                     LRRMNQQARNAEDESHQTGVPYDWYYRTSKWFVDTVALIAVLVLTGWTFHITRLTSLS
                     PDFASMKVTTAIGLLLLAGALLFLNPHIIRPDPSGTRVRNGIVFVLAAMAGLLGLGTL
                     FGYATHVEGERVSPAWTGPATALCFVLLGGAVFASMKQNVRHYVSQSLATVVIFICSL
                     AVLGYLFHKQSLYAIPSYKSMALHTAVSFVLLSLALLCLRPDQGLMKTATAGTAGGSM
                     LQRMVPLMVGMVLIIGWVHLMGETVGLYNSRFSLLMAVGMSVVGFSIILWTVAGSLNQ
                     IDAAKGLSERRLQHAIKQLRASDEQKTRLLYTVSHEYRTPLNGIIGISRFLLNRADGP
                     LTPEQDKQITMMQTGAQQLLVLIDTMLDLDKIKTGKLQPASRRCTVPSLLQSLRVAAE
                     LFPSRPGVSLVIKEPDSLPELMTDDTLLSRILYNFVHNAVKYTEHGTIIVSAESVSNG
                     RAVRFVVRDSGVGIAPEDQARIFDEFVQIRHPLQQGIRGQGLGLALCKRLADVLDAII
                     MVKSQPGKGSTFSVTVPCEQKTSAAMVEDERQEQAQADMAESGTFKSVECRSGSPMSL
                     VRVI"
     gene            complement(4011..>4335)
                     /locus_tag="VN647_03680"
     CDS             complement(4011..>4335)
                     /locus_tag="VN647_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWG96164.1"
                     /translation="LVMTGKLGENLTVEQGVEAARVAVLNGISIVRSEAGSLDRVKQI
                     VKMVGYIASAPGFTDQPQVLNGASDLLVSVFGEAGRHARVAVGAAELPRLAPIEIELI
                     VELRS"
BASE COUNT          903 a         1272 c         1185 g          975 t
ORIGIN      
        1 gtacaagcgc gcttgcgcct cgcgcagcgc ttcttccgct tgcttccgat catcaatatc
       61 ctcaatgatg caaatgaaat gaagcggcct atgacacgca tctcgaagga gcgataccgt
      121 cttattcacc cagatgagcg aaccatcctt tcgtacgtag cgcttttcca ttgaataggt
      181 ttcaatttca cccgccagca tgcgacgtgc ttgcgcccac tctgtcttta gatcgccggg
      241 agaggtgata tccacgaacg tcatagtgag caattcttca cggaaatagc cggtgattgt
      301 gcataaggaa tcgttgaact gtaaccaccg accgtccagt ccgatgtgag cgatgccgac
      361 agccgcattg gcaaaggtgg cgcgatagcg ctcttcgctc tcgcgtagag ccagctccgc
      421 ttctttgcgg tcatggatat cggtgcaggt ccccacccat gaccgaatgc gcccttcagc
      481 atcccgaacc ggcgcccctc tcacacgaaa ccatcgatat gctccgtcgg cgtcgcggcg
      541 aaatcttgtt tcaacgtcgt actcgccgcc tgtctcgacg gctttcttcc acgcgtcagc
      601 caatcttggc gcatcgtcgg ggtgttggac cgtgaaccag ccgccgtgtc tcgtcgtctc
      661 atgcgtcagt cccgtatagt cgtaccaccg ttgattttcg aactccgtct cgccctgccc
      721 gtcggtatgc cacacgaagt gcggcatcgc ttcggccagc atacggaacc tttcttcgct
      781 ctcgcgtaat ctttcttccg catgcttgcg ctccgtgatg tcctggacaa cggcgatgtg
      841 ctgggagagt tcctctccca gcttccggag cggagccaca ttgagcagga cccagacaat
      901 cgacccatcc ttccgcacgt agcgcttttc catggcgatg gaatccgtca gcccgcagcg
      961 gaggtgttcc atgccggcaa gatcctccgc gagatcgtca ggatgggtaa tactcatgaa
     1021 ggtggtcgcc agcatctcgg cttccgtccg ttctacaatc tcacagtatt tccgattgac
     1081 ccgctcgaat cgtcctgtgc gcgaattgat ttgtgccact cccacgccgg cctgttcaaa
     1141 aatggtgcgg tatcggcgct ctgcatcgcg tagcgcgtct tcagcctgct tgcgtacggt
     1201 gatgtccgtg aacaagttca ccgcgccgac gatgtgtcct tcggcatttc gcagaggatc
     1261 gaggtggacg atcacgtggc gccttgtgcc gtcggagcgt tcgaggatga cgtcctgacc
     1321 atgcagcgcc gtgccatctt gcaccgcatc ggccatgggc gtcttgtcat gcggcagcgg
     1381 tgtcccatcc ggccacagca gcttatgcga cccccgaaac tgactttcgc tgtcgcccag
     1441 gttcggcctg cgaccccaca attcggctgc ttgatcgtta taaaacgtga tacgaccgtg
     1501 tcgatcgatc gtatacatgg ccgccggcat gcgctcgatg agagaccgat agcgcatttc
     1561 gctctcggtg aggcgttcga tgagccgctg attgtcccgc tcgtgctggg ccagcttgag
     1621 cagtgcccgc acagtgccga tcaactcctc ttcctccgcc ggttcgacca aataggcgtc
     1681 cgccccgacc tccaggcttt tcacccgatc cggcgcggtg atgcgggcgg cggaggtctg
     1741 caatattttg atgaacttcg tctcaggctg agttttgagc tgctgacaga catcgaaacc
     1801 gctgatatcc gataaattga cgtcgagcag aatgagagca ggccgttctg cggtggcaac
     1861 agtgagtgcg tcggcgccgt tgtccgcttc aatgattcta taatcctgcc ggctgagcaa
     1921 tcgactcttc acgtacaagg ctgacgggtt atcgtcgaca acgagaatgg tcgggtaatc
     1981 ggcggattcg gcggtggtct gagtcatatc accctcacga gactcatcgg agacccggac
     2041 cgacattcga cactcttgaa cgttccgctc tcagccatat ccgcttgcgc ctgctcttgc
     2101 cgttcatctt cgaccattgc cgccgatgtt ttctgctcac agggcaccgt gaccgaaaag
     2161 gtcgagcctt tcccaggctg actcttaacc atgatgatgg catcgagcac atcggcaagc
     2221 cgcttgcaca gcgctaggcc gaggccctgt cccctgatcc cctgttgcaa gggatgacgg
     2281 atttggacga attcgtcgaa gatgcgagcc tgatcttcgg gtgcgatgcc gaccccgctg
     2341 tcgcgcacga caaaacggac agcccggccg tttgagaccg actcggccga gacgatgatc
     2401 gtgccatgct cggtatattt caccgcattg tggacgaaat tgtagagaat gcggctcaag
     2461 agagtatcgt cggtcatgag ttcgggtagg gaatccggct cctttatcac aagactcacg
     2521 ccgggtcgtg aaggaaacag ttctgcggcg acgcgtagac tctgcaacaa tgagggcacc
     2581 gtacatcggc gggatgccgg ttgtagtttg ccggttttga tcttatcgag atccagcatc
     2641 gtatcgatga ggaccaatag ttgctgggca cctgtttgca tcatcgtgat ttgtttgtcc
     2701 tgttccggcg tcagcggacc gtcggcacga ttcagcaaga accggctgat gccgatgatc
     2761 ccgttcaggg gggttcgata ttcatggctc accgtataga ggaggcgggt cttttgttcg
     2821 tcggaggcgc gaagttgctt gatggcatgc tgcagacgtc gctcgctcag cccctttgcc
     2881 gcatcaattt ggttcaagga ccctgctact gtccacagga tgatgctgaa tcccaccaca
     2941 ctcatcccaa cagccatgag aaggctgaag cggctattat acaatccgac cgtttctccc
     3001 atcaggtgga cccatccgat gatgaggacc attcccacca tcagcgggac catgcgttgc
     3061 agcatcgagc cccccgctgt gccggctgtc gccgttttca tcagcccttg atcgggtcgg
     3121 agacagagca gggcgagact cagcaacaca aacgagacgg ccgtgtgtag cgccattgac
     3181 ttgtatgacg gtatggcata gagcgattgc ttgtggaaga gatacccaag cacggcaagc
     3241 gaacagatga aaatcaccac agtggctaga ctttgagaga catagtgccg aacattctgc
     3301 ttcatcgaag caaagacggc tcctcctagg agcacaaagc atagagccgt tgcgggcccc
     3361 gtccatgctg gagaaactct ctctccttcc acatgcgtgg catatccgaa caaggtgccg
     3421 agccccagca gaccggccat cgctgccaat acaaacacga tcccattccg aacgcgcgtg
     3481 ccgcttgggt ccggacggat gatatgcggg ttgaggaaca gcagggcacc ggctaagagc
     3541 agcaagccga ttgccgtggt gactttcatt gaggcgaagt cgggagacaa gctggtgagt
     3601 cgggtgatat gaaacgtcca gccggtgaga accagcacag cgatgagtgc caccgtatcg
     3661 acaaaccact tggaagtccg gtagtaccaa tcatagggca ccccggtctg gtgcgactca
     3721 tcctcggcgt tccgagcctg ctgattcatg cgccgtagac catcccgctc ggaagggagt
     3781 ggcttgggcg tttcttgttt ctctattgat atcgtctctt gataaccaaa tggttcggaa
     3841 tcagtaataa aagcttcggg aggaggatca gcagccttgc cttcatgcat ggacgatact
     3901 ccttggactc gagagaagca tcgaagcaat aaacagctta ctgcatgcca tagtctaggt
     3961 aaacccctag actcgaaaac aaaggttttc actaggacgg tatgtgacta tcacgaacgc
     4021 agttccacta tcagttcgat ttcgatggga gccaggcgtg gcaattccgc ggcaccgacg
     4081 gctacccgtg catggcggcc tgcttctcca aatacggaca caagcagatc agatgcgccg
     4141 ttcagcactt gcggttgatc ggtaaagccg ggagccgagg cgatatagcc gaccatcttg
     4201 acgatctgct tgacccgatc cagcgatccg gcttcgctcc ggacgatact gatgccgttc
     4261 agcaccgcta ctcttgccgc ctcaactccc tgttcgactg tgagattctc tccgagcttg
     4321 ccggtcataa caagc
//
LOCUS       DATNDD010000155         4564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3260182, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000155 DATNDD010000000
VERSION     DATNDD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3260182"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VN647_03685"
     CDS             complement(<1..139)
                     /locus_tag="VN647_03685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HWG96165.1"
                     /translation="MIPLSAYTAVSAVLFMTGILGVLVRRNFIIVLMSVEIMMNAANI
                     NL"
     gene            complement(136..663)
                     /locus_tag="VN647_03690"
     CDS             complement(136..663)
                     /locus_tag="VN647_03690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWG96166.1"
                     /translation="MVGMFFTYFALVSIAAGVLTVTLRHPVHCALSLLVLLMHVSGLF
                     ILLNAEFLWAVQVIVYVGAILVLYLFVLMLMNLKTDERYFHPSALYILGPAVLGAGYV
                     LYLLLRSPFGGAKGDTPATAVLQEGDTYAVGITMFSDHLLQFEIVGIFLLGAIIGAIV
                     LAKAPKPLDTERERL"
     gene            complement(673..1260)
                     /gene="nuoI"
                     /locus_tag="VN647_03695"
     CDS             complement(673..1260)
                     /gene="nuoI"
                     /locus_tag="VN647_03695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWG96167.1"
                     /translation="MSVAALTKKILHAALFYEIWDAMKVTFRHMLHRPMTFQYPREQR
                     TIPDTHRGALGLLRYDDGRERCVGCDLCEVACPSHCIKVISAEDTARPLQRYASEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEYSTHDKRSLLFDKERLYDIGERHLDDGKKYL
                     YAHNQEKNLEQSREYRYYFPQSVQKSTQPPPKHLS"
     gene            complement(1294..3987)
                     /locus_tag="VN647_03700"
     CDS             complement(1294..3987)
                     /locus_tag="VN647_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWG96168.1"
                     /translation="MGLKPATNPDVEAATIELSIDGKSVTARDGVSLYDVIAMTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMVVRTEGEDLFLARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSPVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVAKGDLALDCEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKKTTTTCNYCGDGCQLYLETKDEEVVRVTSSLSWKNKWGDRSETAKGH
                     GGICVRGRFGFEYLDSKSRLTQPLVREGVQLVQKPWLETMHLLVDRFTEIKSKYGADA
                     VGGLVTARCTNEELYLFQKLMRACFGTNQLDSSARYGHMNFVLASRHAVGLGRTPNDW
                     EDLTKAKAIILIGSNITETNPLTAVRIKEAMRIYKAQVVTLDSAITNMAKLASHPFLI
                     KPGTEGAVIDGLVKATIDQDLVDEEVVGKHPQAFEALKSAMGAVSLERLAGQIGFPVE
                     AFREIAAIFAESSRSIILCAEGIVRRTNGYQNVLKLMDLAWITGKLGRAGCGVNTVTE
                     EPNEQGAVDMGAAPEFFPGQARFDDPGVRDRFAKAWEASLPPVGSGAHLIEILKRCKS
                     GQIKALYILGENPLETLPASMEVRAALERLELLVVQDPFLTDTGKLAHFVLSACTYAE
                     KDGTFTNLEGRVLRLRQAMDPLGESLPDWHIMTALANAMGCRWEYQSTNDIQAEIMKL
                     LPGYYNLGQPRQVVPVPDHYLSNGYVAEVKARYQETPPSGERTRPFSLVMGQLLVHSG
                     KLSTQAPGLIKIAPNTGKLRMNIRDMERLGLQDGAKVRLTSDRGSLQLGVQPDQSIAP
                     GTCFFPEHFNEPPVKDLMPVSVDATTGVPSFKQIWVSVEQA"
     gene            complement(4074..>4564)
                     /locus_tag="VN647_03705"
     CDS             complement(4074..>4564)
                     /locus_tag="VN647_03705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWG96169.1"
                     /translation="LRKYEPYSAYPKCEFSVPVGKNGDTYDRYWIRVMELYESVKIIR
                     QCLEQMQDGPIMADVPSVTLPPKERVFTNLEAMIQQFKLFSQGFDAPPGEIYCGTEAH
                     KGELGFYIVSTGSGKPYRLKIRAPSFVHMGAFDHMARGYMISDACTIFGTYDVVMGEC
                     DR"
BASE COUNT         1070 a         1319 c         1133 g         1042 t
ORIGIN      
        1 ctaaattgat gttggccgca ttcatcatga tttcaaccga catcagcaca atgatgaagt
       61 tccgcctcac aagcacaccc aagatccccg tcataaaaag gacggcgctg acggctgtat
      121 aggcggacaa gggaatcata accgctctct ctccgtatcc agcggcttcg gagccttggc
      181 caggacaatc gcgccgataa tggctccgag cagaaagata ccgacgatct caaattgcaa
      241 gagatggtcg ctgaacatcg tgatacccac cgcgtacgta tctccctctt gcagcacggc
      301 agtggccgga gtatccccct tcgctccgcc aaacggcgac cgcaggagga ggtagagaac
      361 gtagccggcc cccaagacgg cgggtccgag aatatacaga gccgaggggt gaaaataacg
      421 ctcatccgtt ttgagattca tcaacatcaa cacaaagagg tacagcacca gaatggctcc
      481 aacatacacg atgacctgta cggcccaaag gaactccgcg ttcagaagga tgaacaggcc
      541 ggagacgtgc atgagcaaca cgagcaggga gagcgcgcag tgaacggggt gtcgcaaggt
      601 taccgttaaa acaccggcgg caatactgac caatgcaaaa taagtgaaaa acattccgac
      661 catatgcgcg gctcagctca gatgtttggg aggtggttgg gtcgacttct gtaccgactg
      721 cggaaaatag tagcggtact cgcggctctg ctcgagattt ttctcctggt tgtgtgcata
      781 caagtatttc ttcccatcat cgaggtggcg ttcgccgatg tcgtacagcc gctccttgtc
      841 gaacagcaga ctgcgtttgt cgtgagtcga gtattcatac atcttggtca tcgccaacgc
      901 gttcaccgga caggcttcga cacaatagcc gcagaaaacg cattttgtga tatcgatata
      961 gaactcactg gcgtagcgct gaagcggtct ggccgtatct tcggcgctga tcactttgat
     1021 gcaatgcgaa gggcaggcga cctcacagag atcgcaacca acgcagcgtt ctctcccatc
     1081 atcatatcga agcaagccaa gcgcaccgcg atgcgtatct gggatcgttc gttgttcccg
     1141 cggatactga aacgtcatcg gacgatgaag catgtgccga aacgtgacct tcatcgcgtc
     1201 ccaaatctca tagaataacg cggcatggag gattttcttg gtcagcgcgg cgacactcat
     1261 cgtaggctcc tcgtcacgga cgtagatatc cgcttacgcc tgttcaacac ttacccagat
     1321 ctgcttgaac gacgggacac cggtcgtggc atcaaccgac accggcatca agtccttcac
     1381 cggaggttca ttaaaatgct cggggaaaaa acaggtaccc ggtgcaatgg actgatccgg
     1441 ctgcacaccg agctgaaggg aaccacgatc cgaggtcaaa cgcaccttcg caccgtcttg
     1501 caatccaaga cgctccatat cccgtatatt catccgcagt ttcccggtgt tgggagcgat
     1561 cttgataagc ccgggcgctt gcgtagacaa tttccccgaa tgcactaata actgccccat
     1621 caccagcgaa aagggacgtg tgcgttctcc cgatggtgga gtctcttgat aacgcgcctt
     1681 gacctccgct acgtagccgt tggacaaata atggtcaggg acgggcacga cttgacgcgg
     1741 ttgtccgaga ttatagtacc cgggtaacag cttcatgatc tcggcttgaa tgtcattggt
     1801 cgattggtat tcccatcggc accccatggc attcgcaaga gctgtcatga tgtgccagtc
     1861 cggcaggctc tctccaagcg gatccatcgc ctgacgaaga cgaagcacac gaccttcaag
     1921 gttcgtaaat gtgccgtcct tctccgcata ggtacaagcg gagagcacaa aatgagccaa
     1981 tttccccgta tcggtcaaaa acggatcttg cacgactaat agttcgagtc gttcgagagc
     2041 ggctctgacc tccatcgaag ccggcaaggt ctcgagcgga ttttctccca gaatgtacag
     2101 ggctttaatc tgcccactct tgcatcgctt caagatttcg atgagatggg cccctgatcc
     2161 gacaggaggg agcgacgctt cccatgcttt ggcgaaacgg tcccgaactc ccgggtcatc
     2221 aaaccgagcc tgacctggga aaaactcagg tgccgcgccc atatccacag ccccttgttc
     2281 attcggttcc tccgtcactg tgttcacacc acaccctgct cgtccgagct tgccggtgat
     2341 ccaggcgaga tccatcagtt ttaagacatt ctgatagccg ttcgtccgcc tgacaatccc
     2401 ttccgcgcac aggatgatag acctcgacga ttcggcaaag atagccgcaa tttctcggaa
     2461 ggcttctacg ggaaagccga tctgacccgc tagccgctcc aacgaaacag cccccatcgc
     2521 gcttttgagc gcttcaaatg cttgagggtg tttcccaaca acctcttcgt ccaccaaatc
     2581 ttggtcgatg gtcgccttga ccaacccgtc gatcactgct ccctctgtcc caggcttgat
     2641 taaaaacggg tgcgaggcca acttcgccat attcgtgatg gcgctgtcga gcgttaccac
     2701 ctgagccttg tagatccgca tggcttcttt gattcgcacg gcagtcaatg ggttcgtctc
     2761 ggtaatattg gagccgatca aaatgatggc tttcgccttc gtcagatctt cccaatcatt
     2821 cggtgttctc ccaagtccga cggcatgtct ggacgcgagc acaaagttca tatggccgta
     2881 gcgggcgcta ctatcgagct gattagttcc aaaacaggcg cgcatgagct tctggaacaa
     2941 atacagctct tcattcgtac agcgggccgt cacaaggccg ccgacggcat cggcaccgta
     3001 tttgcttttg atttcggtga accggtctac gagcaggtgc atggtctcca gccaaggctt
     3061 ctgcaccaac tgcacccctt cgcggaccag aggctgtgta agccgactct tactgtcgag
     3121 atattcgaat ccaaaacgcc cgcgtacgca gatgccgccg tgccctttgg cagtttccga
     3181 tcggtctccc catttgttct tccaagataa cgaggaggtg actcggacca cctcttcatc
     3241 tttcgtttcc agataaagtt ggcaaccgtc tccacaatag ttgcaggtcg tcgtcgtctt
     3301 cttcatctgc cacggcttat acagatattt ggaaaacttg ttcgtgatgg cgccgaccgg
     3361 acagaccgct aaacaatctc cgcagaattc acaatcgagt gcgaggtcgc ccttggccac
     3421 gacctggttg aatccaccct tcttcatgaa ctgcaaggca tcgatcatca acacatcctt
     3481 gcaaacattg atgcattcgg cacaggcaat gcagcgattc atgttgaagt cgagcacggg
     3541 actgcgggta tcttccggaa tgaatttctg cttggcattg gccagattgg tcacgccatg
     3601 ctggaacgcc atatcttgaa gttcacaatg tccgtccgca tcgcatacgg ggcagtcgag
     3661 aggatgcaca gacaggtgtt tctcgacagc tttcttccgg gcgaggaaga gatcctctcc
     3721 ttctgtccga accaccattc ccgcggccgc cttggcggtg cacgaccgaa cgggagcctt
     3781 cttcccttcc tgcatgacga ggcacatgcc acacgagccg aacgggtcga atgtgtagtg
     3841 atagcacatg gccgggatga tcttgcccgt cattgcgatg acgtcgtaca acgacacccc
     3901 gtcccttgcc gtgacagatt tcccgtcgat gctcaattcg atcgttgccg cttcaacatc
     3961 tggattagta gccggcttca aacccatagt tcttctccgt gaaagggaaa acgtgaaccg
     4021 acaaatgaaa tcctgatttt acctttcact ttccacatct catatttcac attctatcta
     4081 tcgcattcgc ccatcacgac atcgtacgtc ccgaagatgg tacaggcatc cgagatcata
     4141 tatcctctgg ccatatgatc gaacgcgccc atgtgcacga acgaaggggc gcgaatcttc
     4201 agtcgatagg gctttccact ccctgtactg acgatgtaaa accccaattc tcctttgtgc
     4261 gcctctgtgc cgcagtagat ttctccaggt ggagcatcga acccctgcga gaaaagcttg
     4321 aactgttgga tcatcgcctc taggtttgta aaaacccgtt ctttcggcgg gagcgtcacg
     4381 ctgggaacat ccgccatgat gggcccgtcc tgcatctgct ccagacattg ccgaatgatt
     4441 ttgacgctct catagagctc catcacccga atccagtagc gatcgtacgt atccccgttt
     4501 tttccgaccg gcacgctgaa ctcacacttt ggataggcac tatagggctc atacttgcga
     4561 agat
//
LOCUS       DATNDD010000156         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1395950, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000156 DATNDD010000000
VERSION     DATNDD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395950"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..832)
                     /locus_tag="VN647_03710"
     CDS             complement(89..832)
                     /locus_tag="VN647_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWG96170.1"
                     /translation="MLDSLASAFAWEGIAIYWGIGLLFFAAEYWWSTRPVPYLQVFLP
                     DVAALTTYQVFFVFAAGVTNRIPFPHYSYWRWQALPFGFKLIVFLFVVDGIAYWMHRL
                     WHTSWGWPIHRWHHAPTELYWLAGVRASFPQIVLANVPYLLAFPLLKPVPAAFFPIYG
                     YMLVLTNNWMHMNVTWQSRKLEWLFVTPRYHRVHHLREIAKAGANFGVLFTVWDRLFG
                     TYVDPEQADLTGPYGIQETVHPVRMAAGV"
     gene            complement(1019..2110)
                     /gene="ychF"
                     /locus_tag="VN647_03715"
     CDS             complement(1019..2110)
                     /gene="ychF"
                     /locus_tag="VN647_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWG96171.1"
                     /translation="MGLCCGMIGLPNVGKTTVFNALTGGGALAANYPFATVDPNTGIA
                     LVPDPRLIKLTEIFTSKKTTYSTLEVRDIAGLVEGASKGEGLGNQFLGHIREVDALLH
                     VVRCFQGTDVVHVSGGIDPLRDIGVIETELMLSDLETLDRRKQKTEKKVRAGDKKAAF
                     EVEFLAKLIGLLDKGEWLGNREYTVEERVILNECQLLSAKPVLFVANVSEGKNADEVI
                     VQAVREFAAKRGARVVTICGQLEAELSSLPESERADFLNELGLTESGLVRLTREAYTL
                     LDLITFFTAGEIESRAWPIPKGTKAPQAAGKIHSDMERGFIRAEVYHYDDLLTCGSEA
                     KVKEKGLFRLEGKDYVIKEADVVYFRFNV"
     gene            complement(2245..>2789)
                     /gene="pth"
                     /locus_tag="VN647_03720"
     CDS             complement(2245..>2789)
                     /gene="pth"
                     /locus_tag="VN647_03720"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWG96172.1"
                     /translation="TRHNVGMWVIERAAARWSIRLSPRGTTQRGSGRLGGKLVELAGL
                     LDWMNISGPPLKGLLREFELTTSEFVVVHDDLDLEPGRLRIKLAGGHGGHNGIKSVID
                     ALGTPQFMRLKIGIGRPAPSQDSADYVLEPVTKDEMAVFDPCLERAVDALECLIHRGP
                     EVAMNQFNVRERVDEGEQPD"
BASE COUNT          645 a          798 c          722 g          624 t
ORIGIN      
        1 taattgatcc tgagtctttt ctataggccg attactggtc acgtcggcga atcacgtctg
       61 ttgaacctga ttaatgattg cttctggttt aaaccccggc tgccatacgg acaggatgga
      121 cggtttcctg aataccatac ggccctgtca agtccgcttg ctccggatca acataggtcc
      181 cgaacagccg atcccatacg gtaaacagga ccccgaaatt cgcccctgcc ttcgcaatct
      241 ctctcagatg atgcacacga tgatagcgag gcgtgacgaa gagccactcc agttttctcg
      301 attgccaggt cacgttcatg tgcatccagt tattggttag gaccaacata tagccataga
      361 tcgggaaaaa cgctgcaggc accggtttga ggagtgggaa cgccaaaaga tatggaacat
      421 tcgcgagcac gatttgtgga aagctcgccc ggacaccggc cagccagtat agttcggtgg
      481 gcgcatgatg ccatcgatgg atcggccagc cccacgacgt atgccacagc cgatgcatcc
      541 aataggcgat tccgtcgacg acgaacaaga acactattag cttgaaaccg aacggaagcg
      601 cctgccaacg ccaataggag tagtgaggaa acgggattcg gttcgtcacc cctgcggcaa
      661 agacgaagaa gacttggtac gtggtgaggg ctgctacatc cggtaggaat acctggagat
      721 atgggaccgg acgtgttgac caccaatact ctgccgcgaa aaagagcagg ccaattcccc
      781 aatagatagc gatcccttcc cacgcaaacg cactagcaag cgagtcgagc attgttgtga
      841 cctaggacac atgccttaga gtgacgacac aaaaagcaag acgagaggat cgctcatctc
      901 aaagacaggg tcaaggtcag catggtactt tattcaccat cttatggggg gagcatcaat
      961 tcagaagacg gacaaagtcg ggaaggttgg agagcgagaa ttgcggtctt ttggatggct
     1021 aaacgttaaa cctaaaatag acgacgtccg cttctttaat gacgtagtct ttcccttcga
     1081 ggcggaacag tcccttctcc ttgaccttcg cttccgaccc acaggtgagc aaatcgtcat
     1141 agtggtagac ttctgcacga atgaagcctc gttccatatc ggagtggatc ttgcctgcgg
     1201 cttgaggcgc cttggtgccc tttggaatcg gccaggctcg agattcgatc tcgccggcag
     1261 taaagaaggt aatcagatcc agtagagtat aggcttctct agtcaatcga acgagccctg
     1321 attcggtcag ccccagttcg ttcaggaagt ccgcccgttc gctctcaggc aatgacgaca
     1381 gctcggcttc cagctgtccg cagatggtca cgacccgcgc cccgcgcttc gcagcaaact
     1441 cgcgcacggc ctgcacgatg acctcgtccg cattctttcc ttcggacacg tttgcgacaa
     1501 acagaaccgg cttagcggag agcaattgac attcgttgag gatcacacgt tcttccaccg
     1561 tgtattcccg attgcccaac cattcacctt tgtcgagcaa tccgatgagc ttggcaagga
     1621 actccacttc aaacgcagct tttttatcac cggcccggac cttcttttca gtcttctgtt
     1681 ttcggcgatc gagtgtctcc agatcggaca acatgagttc ggtttcgatc acgccgatgt
     1741 cacgaagcgg atcgatcccg ccgctgacat gaaccacatc ggtgccctga aaacagcgga
     1801 ccacgtgaag aagggcatcc acttctctga tgtggccaag aaattgattg ccgaggcctt
     1861 ctcctttgct ggccccttcg acaagtccgg caatgtctcg tacttccaac gtgctgtagg
     1921 tggttttctt cgaggtgaat atttcggtca gtttgatgag gcgggggtcc ggcaccaggg
     1981 caataccggt gtttggatcg acggttgcaa acggataatt agccgccagg gcacccccac
     2041 cggtcagtgc attgaagacc gtcgtcttgc cgacgttcgg cagaccgatc atgccgcagc
     2101 aaagtcccat gttatttcct tgtctttcta cacacttttt acaaagggga gctgcggggc
     2161 tgacctaact gcgcgcatcc accgcgcacc gcccttagtt tcgtcattcg atccattctc
     2221 gcgtgcgcgg tgggcgagca caggtcagtc cggctgctcc ccttcatcta ctcgctctct
     2281 gacattaaac tgattcatcg ccacttcggg tccgcgatga atcaaacatt ccagtgcatc
     2341 cactgcccgt tccaggcagg gatcaaagac agccatctca tccttcgtca ccggctctaa
     2401 aacatagtcg gccgaatctt ggctcggagc aggacggccg atcccgatct tcaatctcat
     2461 gaattgcgga gttccaagcg cgtcgatgac cgacttgatt ccgttgtgtc ccccatggcc
     2521 accggcaagc ttgatccgca gccggcccgg ctctaaatcc aaatcatcgt gtacgacgac
     2581 gaattcgctg gtggtgagct cgaattctcg taggagacct ttcaacggag ggccggaaat
     2641 attcatccag tcgagcaggc cagccagctc gaccaacttc cctcccaggc gtccggaacc
     2701 tcgttgagtt gtgccgcgcg gcgagagtcg gatcgaccat cgagcggccg cccgctcgat
     2761 gacccacatg ccgacattgt gacgggtct
//
LOCUS       DATNDD010000157         2371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2794277, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000157 DATNDD010000000
VERSION     DATNDD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2794277"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1522
                     /locus_tag="VN647_03725"
     CDS             257..1522
                     /locus_tag="VN647_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96173.1"
                     /translation="MRWLKRILIGLAVLSGLTYLYYTEVKPTVIFGLREDYAHAIPHQ
                     KVPEGLTSLRAESCGTCHTEIYNEWKTSIHSQAYKDPFFHAYWTKDKHTWVCLNCHSP
                     LENQQPTLIKNIPRDRVERAVQEPNPHYDQEYQQEGVTCAACHVRDGVILGPFEDAKA
                     PHPTKYDPMFRTTQLCYRCHSVVGGPAQFYNGGPCGTYPEYEDGYWKKERGFICQNCH
                     MPEIERPVAINGPIRQGRQHLWRGGHDPAMVKRAIDVNVVADPAEPKPGDTVRVTLTL
                     INAGAGHKLPTGDPDRHFTVEFAVEDQNGKVLEQQSDTMGRWIMWQPAIVELYDNRLM
                     PLASRDYIFEYRLPKESAGLKLTTKVRYHIQTERQHRMLIDKYGLTAKDPYNFTVYER
                     AIALTGNLTDHFQNNEAHSHMACAAANHS"
     gene            1640..2266
                     /locus_tag="VN647_03730"
     CDS             1640..2266
                     /locus_tag="VN647_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWG96174.1"
                     /translation="MKEIHSWSGLFVGWLLFAVSLTGALTVFDNEITYWMQPGLLEVS
                     TGARELDKTVPRVTESLMQVAHWNIAIPTERSPALFVKLQEKQTFSGQTINPATGEMN
                     SFRDTQGGDFFYHFHDGLLSGWHGAWLVGIAAIVMLVTLVNGLTTKSHLLTSVPMGQW
                     AMAGVDLTGVTAGMLLGWAARRVARAAGEIPRNHTASGIVSDQRPAQM"
BASE COUNT          596 a          650 c          635 g          490 t
ORIGIN      
        1 tcatatgtag atgtgtacat atattctgat gcagaagacc agaagcgcat ccctccctat
       61 tccacatgac ctttggtctc aagatgtcat cgtatcccgc tcagttgtta atgctccctg
      121 acgaaaccgt tcgcagaccg aactcaccaa caatatcttc atcctccatt acgggaaaac
      181 tcgtcacagt ccgagcgctc ataaaaactg agattctgaa atccatgaac ctcgggctgg
      241 aacaacggag atctcgatgc ggtggttgaa gcgaattctg atcggactcg ccgtcttaag
      301 cggccttacc tatctatatt acaccgaagt gaaaccgacc gtcatcttcg gcttgcgaga
      361 agactatgcc cacgctattc cccatcaaaa ggtacctgag ggtctgacga gcttgagagc
      421 agaatcgtgc ggcacgtgcc acacagaaat ttataatgaa tggaaaacca gcattcactc
      481 acaagcgtat aaagacccct tcttccatgc ctattggacg aaggataaac atacgtgggt
      541 ctgcctcaat tgccattcgc cgctggagaa ccagcagccg accctgatca agaacattcc
      601 gcgagaccga gtggaacgtg ccgtccagga gccaaatcca cactatgacc aggagtatca
      661 gcaggaaggc gtgacctgtg cagcctgtca tgtgcgggat ggagtgatcc tgggaccttt
      721 cgaggacgca aaagctcccc atcccacgaa gtacgatccc atgtttcgta ccacgcaact
      781 ctgttaccgc tgccatagcg tcgtgggcgg accggcgcag ttttataacg gtgggccttg
      841 cggcacctat cccgagtatg aggatgggta ctggaagaaa gagcggggct tcatctgcca
      901 aaactgccac atgcccgaga tcgaacggcc ggtcgccatc aatggcccca tccgccaggg
      961 tcgccagcat ttatggcggg gtggacatga ccccgcaatg gtcaagcggg ccatcgatgt
     1021 caacgtcgtg gccgacccag ccgaaccgaa gcccggcgat acggttcgag ttacgttgac
     1081 tctgatcaat gcgggtgcgg gacataaact cccgacaggg gatcccgatc gccacttcac
     1141 cgtcgagttt gcggtggagg atcagaacgg gaaagtcttg gagcagcagt cagacacgat
     1201 ggggcgatgg atcatgtggc aaccggccat tgtcgaactc tacgacaatc ggctcatgcc
     1261 gttggccagc cgagattata tattcgagta ccgactcccg aaggaaagtg ccggcctcaa
     1321 actgaccacg aaagtgcgct accacatcca aacggaacgg caacatcgga tgctcatcga
     1381 caagtacggc ctcacggcga aagacccgta caattttacc gtctatgaac gagcgatagc
     1441 tctcaccggc aatctgacag accattttca gaacaatgag gcgcactcac acatggcatg
     1501 tgccgccgca aatcacagtt gaagcacaaa cctgacgccc gggcatggtt cccttctcag
     1561 aaaatgttga ggacatatca gaagggaccc atcatcgccg taatcaagga gaggccaggc
     1621 aaggcggttt acacaatcga tgaaagagat acattcttgg agcggtctct tcgtgggctg
     1681 gctgttgttc gcggtttctc tgaccggggc gctgaccgtt tttgacaatg agatcaccta
     1741 ttggatgcag ccggggttac tggaggtgtc caccggtgcg cgagagttag acaaaactgt
     1801 cccccgtgtc accgagtcgc tcatgcaggt ggcgcactgg aatatagcta ttcccacaga
     1861 gcggtcccca gcgctcttcg tcaaattgca ggagaaacaa actttttcag ggcaaacgat
     1921 caaccctgct accggagaaa tgaatagctt ccgtgatacc caaggcggag atttctttta
     1981 tcactttcac gatggattac tatccggatg gcacggcgct tggctggtcg gtatcgcggc
     2041 gatcgtgatg ctcgtcaccc tcgtcaatgg cctgacgaca aagagtcatc tcttgacgag
     2101 cgtaccaatg ggacaatggg caatggctgg tgtcgatctc acgggggtta cggcaggcat
     2161 gttgctcggc tgggcggccc gacgtgttgc cagagctgca ggggaaatac cccgtaacca
     2221 tactgcttcg ggcatcgtgt cggaccagag acccgcgcag atgtgacgag gccgctgctg
     2281 gttatgaggc ccacggatca cgaggacgtt caggagctcc tctgcgagta caggccataa
     2341 tgcgaaatga ctacacgaag gaataattca a
//
LOCUS       DATNDD010000158         5077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3668226, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000158 DATNDD010000000
VERSION     DATNDD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668226"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1609)
                     /locus_tag="VN647_03735"
     CDS             complement(5..1609)
                     /locus_tag="VN647_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HWG96175.1"
                     /translation="MTTETTPLSELAAAIARRRTFAIISHPDAGKTTLTEKLLLYSGL
                     IRTAGMVRGRKGGKTTASDWMGMEQERGISITASAMQFPYKDAVVNLLDTPGHQDFSE
                     DTYRTLTAADSAIMVIDAAKGVETQTRKLFAVCRMRRIPVLTLINKMDLPGRPPLDLM
                     TEVEQALDIHASAINWPIGSGSDFVGIVSRTDNRVQLCSKTMRGGATKVETNTLSLAE
                     LHSNGRVSQETMAQVQHDLELLEIAGNPFTRDQFLQGAVTPVFFASALTNFGIESFLD
                     TFVDLAPSPGMRPADSDDGSELFINPMNMPFSAYVFKLQANMNPKHRDSTAFLRICSG
                     RFERDMVVKHHRLGRDIRLARPHSLVAQERSTVEEAYPGDIIGIINPGVFAIGDTVSL
                     TGGFNFKSLPQFQAELFARLRPTDVGKRKAFDKGLSQMAQEGTVQIMRSLNDQDALIA
                     AVGRLQFDVLQYRLRHEYRVETIMDALPFTCSAWLDGDPSTFKSPSASMMVKDQRDRV
                     VVLFGDQLMKTIARDRNPDHVLRDMG"
     gene            complement(1752..2327)
                     /locus_tag="VN647_03740"
     CDS             complement(1752..2327)
                     /locus_tag="VN647_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWG96176.1"
                     /translation="MVRDPSVGYTAFSYKTVMQPRGYEPGSVTRFLAEDHRRLEHLLQ
                     TALASADHVVQETYDHFRAGLLRHIGMEEKILLPAVQRWNEGRPLSIAAKLRLDHGAL
                     AALLMPTPTTDIIGTIHRILEQHNLAEEGPDGWYEICDRLAGAEADQLMTRLRATPEV
                     AVLPHSDTAAVLSAVHRALERAGHTLGSGSR"
     gene            complement(2774..2974)
                     /locus_tag="VN647_03745"
     CDS             complement(2774..2974)
                     /locus_tag="VN647_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96177.1"
                     /translation="MEAGQTLLALIGACTSFVTFCLMRFPQTRRSRFQRYGPFSLIAS
                     FFGALTILLISALTVYAQAVWH"
     gene            complement(2995..3222)
                     /locus_tag="VN647_03750"
     CDS             complement(2995..3222)
                     /locus_tag="VN647_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96178.1"
                     /translation="MAALWKGRVTEAIQMVRLEQNIGLAEATDLIDTFLRSQPALKSQ
                     INEVQADAREGLLRWLIFLLTGGVGLAHFLG"
     gene            complement(3571..3834)
                     /locus_tag="VN647_03755"
     CDS             complement(3571..3834)
                     /locus_tag="VN647_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96179.1"
                     /translation="MKYSEGETVPDWWFHDFEPVWRSFIQSLGRQKKVTMLEFEQAAS
                     QVPGLTHIEKTIAISAFRQQQEQRSAPSHSTAAVRSERHLSVV"
     gene            complement(4580..>5077)
                     /locus_tag="VN647_03760"
     CDS             complement(4580..>5077)
                     /locus_tag="VN647_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWG96180.1"
                     /translation="YRKTIRGYTPQAVDALRAYPWPGNVRELSNRIRRAVVMAEGMEI
                     TPHDLDLAAEELQTADSLDSLRAAHRRIEMELLVKAISLHRGNLTRMARDLEVSRSTL
                     YRKLRAYGLEKFVPAATSLSLRSRSKTKKSAKLSDQESAGEMPSESIPVDLPLESADQ
                     IPQSV"
BASE COUNT         1189 a         1347 c         1339 g         1202 t
ORIGIN      
        1 cagtctaccc catatcccgt agcacatgat caggattccg gtcgcgcgcg atggttttca
       61 tgagctgatc gccgaagagg acgacgactc gatcccgttg gtccttcacc atcattgagg
      121 cagatggtga cttgaaggtg gagggatctc cgtccagcca cgcactacag gtaaacggca
      181 gtgcgtccat gatggtttcc actcggtact catggcgcag tcggtattgc agcacatcga
      241 actggagacg accgaccgct gcaatcaggg catcctgatc attgagactt cgcatgatct
      301 gtactgttcc ctcttgcgcc atttgggaca ggcccttgtc gaacgctttg cgtttgccca
      361 catccgttgg cctgagacgt gcaaaaagtt cagcctggaa ctgcggcaga gatttgaaat
      421 taaaccctcc ggtcaatgaa accgtatcac caatggcgaa gaccccggga ttgatgattc
      481 cgatgatatc gccgggatag gcttcttcga ccgtactacg ttcctgcgcc accagactgt
      541 gtggtctcgc cagtcgaata tcgcgaccca atcggtgatg cttgaccacc atgtctcgtt
      601 cgaaacgtcc ggaacagatc cgaagaaagg cggtgctgtc tcgatgtttg gggttcatat
      661 tggcttgcag cttgaacaca taggcgctga acggcatgtt catcgggttg atgaaaagct
      721 cggaaccatc atcgctgtcg gcaggccgca tacctggact tggcgccaga tctacaaacg
      781 tatcgagaaa gctttcaatg ccgaaattcg tgagcgcgga tgcgaaaaag accggggtga
      841 ccgctccctg cagaaattgg tcccgcgtga agggatttcc ggcgatctcc aaaagctcca
      901 aatcgtgctg cacctgtgcc atcgtctcct gcgagactcg accgtttgaa tggagttcag
      961 ccaaggacaa agtattcgtc tccactttgg tcgctccgcc acgcatggtt ttgctgcaca
     1021 gctgtacccg gttgtcggta cggctgacaa tgcctacgaa gtcactgccc gacccgatcg
     1081 gccagttgat cgcgcttgcg tgaatgtcca atgcctgttc cacttcagtc atcaagtcga
     1141 gcggtggacg ccccggcagg tccattttgt tgatcaacgt caggacgggg atccgccgca
     1201 tgcggcacac ggcgaataat ttgcgtgttt gggtttccac gcctttggcc gcatcgatga
     1261 ccatgatggc actgtctgcg gccgtcaagg tgcgataggt atcttcggaa aaatcttgat
     1321 ggccgggcgt atccagtaag ttcaccacgg cgtctttata gggaaattgc atggccgacg
     1381 ccgtgatgga aatgccgcgc tcctgttcca ttcccatcca atccgaagcg gtggtcttgc
     1441 cgcccttgcg tcctcgcacc atgcctgcgg ttcgaatcag gccggagtac aagagaagct
     1501 tttcggtcag cgtcgtttta ccggcatccg gatggctgat gatggcaaag gtgcgcctcc
     1561 gtgcgatggc ggcggcaagt tcgctgagtg gagtcgtttc agtcgtcatg atgcgaggcc
     1621 cggcacacca tatcgtgttt aaaaggggat gattctcaca cggtgagtga gtcgagccgt
     1681 tgagtcggtc cggttgattc gagttcgaac aacgattgga atttccttga accttctcct
     1741 gactccgttg ttcagcggga acccgagccc aacgtatgac cggcccgctc caatgcacga
     1801 tggactgcac taaggacggc cgcagtatcg gaatgcggga gcaccgccac ctccggggta
     1861 gcacgaagcc tggtcatcag ttgatcggcc tcggcccctg ccaatctatc acagatttcg
     1921 taccaaccgt caggaccttc ttcggcaagg ttatgctgct ccaaaattct atggatcgtg
     1981 cctatgatat cagttgtggg agtcggcatg agtaaggctg caagggcccc atgatcgagc
     2041 cgaagcttcg ctgcgatcga aagcggcctt ccttcgttcc atcgctgtac cgccggcaga
     2101 agaatcttct cttccatccc gatgtgccgg agcaggccgg ctcgaaagtg atcgtacgtt
     2161 tcctgcacca cgtggtccgc ggaagcaagg gctgtctgaa gaaggtgctc taagcgccgg
     2221 tgatcctccg cgagaaagcg tgtaacggac cctggttcat atcctcgtgg ttgcatcacc
     2281 gttttatatg agaaagctgt gtacccgaca gagggatctc gcaccatagc ttgaagatat
     2341 tcatgacgga ttcgaacgaa aaacacaaga tatcggctgg tacaaggctg actgcggata
     2401 aactgcaaac cctacaatgc ctagatcgac atgccggcgt ccaggtggaa actatactcg
     2461 atgtcatggc ttcgataacg ttgcgatgaa ccttgacagc gggaggcagg ataaacatga
     2521 ctgttgatta tgcggcttca tggagtttta tcgccgcatt acccctgcca gatgttttct
     2581 acagcttctt gccccgttcc gaaaagccag atgaccaact ttcccatgca agtggcaagt
     2641 cctacaacca ttaaaccgat aaccacgatg attccgatgc cgagccaact ctcggacata
     2701 gcgaacatag gtatcccttg agcaacgact tacctttgta gattgtacac cttgtcggag
     2761 aaggaagaaa atttcagtgc cagacagctt gtgcatagac cgtcaaggcg gagatgagga
     2821 ggatcgtcaa ggcaccgaag aaggaggcta tgagcgagaa tggaccgtat cgttggaacc
     2881 gagatcgccg agtctgcggg aaccgcatga gacaaaatgt cacgaatgat gtgcaggctc
     2941 cgatcaatgc cagcagagtt tgaccagctt ccattgtgcc aatctttcca ttacttaacc
     3001 caaaaaatgt gcaagcccta caccaccggt caacaggaag atcagccaac ggagcaatcc
     3061 ttctcgcgca tcagcttgaa cctcgttgat ttgactcttg agagccggct gtgaccggag
     3121 aaatgtatcg atcaagtccg tggcttcagc taaaccgatg ttctgctcaa ggcgaaccat
     3181 ctggatggct tcagtaaccc ggcctttcca aagtgcagcc acagccgctt ctgagagtgc
     3241 aggatacagc ggaggagctg tctgagcgtc agagattgga gactgaagag atacaagcgg
     3301 cataggctga aacacagcct agcaccatcg tccgagcagc cgaagaaaaa ccgaagaatt
     3361 gacgaatgaa aacccgaaac gtccctcgag ttcagctagg cccttcgcgg gcggtattcc
     3421 caggaacacg cagtcggtag ccgcacaaga acgaggcatc accgtatcgt ctctatcgtt
     3481 gtgtagaatc gagccggccg gttattcagc tccaaacgac acacggaccc cctgccctgg
     3541 aaccagctca aaacgaaagt cggtagtcgg ttacacgaca gacaggtggc gttcactgcg
     3601 aaccgcagca gtgctgtggg aaggcgctga tctctgttct tgttgttgac ggaacgcgga
     3661 tatggcgatt gttttttcga tgtgtgtcag accgggaact tgagaagccg cttgttcaaa
     3721 ctcaagcatc gttaccttct tctgacggcc gagcgactga ataaatgatc tccagaccgg
     3781 ctcaaagtca tgaaaccacc agtccggcac cgtctcccct tctgagtatt tcatagatga
     3841 cagcggggta cagcccaact catactgatg gtgcgcagcc gttggtcgat gcagaagtaa
     3901 ggatttgttc tttccgcttt atgttattca caatgtccca ccccctcaaa gaccgcacag
     3961 aaggttgctt cttgagcaat tgagatgcca cagacataat attgttattt gataatgtgt
     4021 tgcgaaatat tttgctgatg gtgatgagat cgattgtatg aaatcgtacg cgtttgtctt
     4081 attctggaac actgatttca ctcaaacgcc ccctctcttt tctcgctgcc cccacgctct
     4141 tcattccaaa aagttactga atgcctagaa ttttgaaaac ctgacggata ctttttcatc
     4201 atgttgacga atatgattta gtgcaaagtg ttctattatt agacacaccg gagaaagttt
     4261 tttgaaatgg acacagcggc tgctagaggt tgatttgacg aaattatacg aactatcttg
     4321 aagatctacc ggatgttgcg gcattgtcct tcttctttat ggtgtttcat gatgaaacac
     4381 ttcttggata tccctgcttg cggttcagat ttttcaagcg cgagcggatg tgttcgctcg
     4441 cttccggata cacgcacctt tccacgcata gaggcctatg cgctttctgt ttcagttgcc
     4501 gtcgcggtca agagagatgt cagaggcggg taaaggatat ttttcaagaa agagactcgt
     4561 tcataacgaa acagtcctgt tagaccgatt gagggatctg atctgccgac tccaagggaa
     4621 ggtccaccgg tatagattca gacggcatct cgcccgcgct ttcttggtcg gagagttttg
     4681 ccgatttctt ggtcttggat ctggagcgta aagaaagcga agtcgcggcg ggcacaaact
     4741 tttcgagccc ataggcccgc agttttcggt acaatgtcga gcggctcacc tccaagtctc
     4801 gggccatccg ggtcaagttt ccccggtgta aactgatcgc cttcaccagc aactccattt
     4861 caatccggcg gtgagccgcc cgcagagaat ccagtgaatc cgctgtttgg agttcctcgg
     4921 cggccagatc caaatcatgc ggagtaatct ccatcccttc cgccatgacg acggcgcgtc
     4981 ggattcgatt gctgagctcc cgcacatttc ccggccaggg ataggcacgc agcgcatcga
     5041 cggcctgcgg cgtatagcct cggatcgtct tgcgata
//
LOCUS       DATNDD010000159         3329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3216548, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000159 DATNDD010000000
VERSION     DATNDD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216548"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VN647_03765"
     CDS             <1..693
                     /locus_tag="VN647_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWG96181.1"
                     /translation="RARADLDVAQAQIARTKAIIAKKTIRAPFRARIGMADVHPGQYL
                     DEGTLLTTLQGVGEAVHVDFTVAQQVAARLQVGETVEVFAAGDAPTVIAKIVALDARV
                     DPITRNAMIRARIEGTRNVPAPGASVRVRIPVGPEHSVIAIPVSALRKGPGGDQVFVI
                     APDKDGRNRVHTRQVESGPMFGDEIVIQAGLTTGEQIAAVGSFKLRDGILIAVAESVE
                     NNSKENHETGKP"
     gene            936..1565
                     /locus_tag="VN647_03770"
     CDS             936..1565
                     /locus_tag="VN647_03770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96182.1"
                     /translation="MHHRLSRKSLDALLVLVLALTGCTGWSSQPINDPSRDTWQKPQG
                     VVEKLSIARGSRIADLGAGGGYFTWHLAKAVGARGTVYAVDTEERAINTIFQEMVSRG
                     TPNVRPVRAEPRDPKLPEPVDLVFSCDTYHHMIDRVAYFRSLASSLRPGGRVAILDLH
                     PRGFVSGLFGDRVGKEEVRREMEDAGYLLIDDFDLIDRQHFQIFAKSDN"
     gene            1751..3274
                     /locus_tag="VN647_03775"
     CDS             1751..3274
                     /locus_tag="VN647_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG96183.1"
                     /translation="MRINAQFRESLLIGFIILLFIGIFIFDIYTPTAFTDHALYVIVV
                     LIATASRFSWMPPLAAGAGTLLTVVGGLGTPWFTDLPRWIQMGNRSITIMVLWVLVWF
                     AWKRRQAKAALQKANEELEQKVAERTQELATVNRTLVTEIAEHVQTEQALRLSQGRLA
                     DILDIAEDAIIVTEHDRSIMLFNQGAVKLFGYDSDEVLGNRIDLLLPERFRIDHPPYI
                     NAFAHNPESARWMAQRREMFGLKKDGSEFPAEASISKLNLGERTTFTVIVRDITDRLR
                     TERQLQSLTAELMTAQEEERRRIARELHDDVNQRLALLAIDMANMLSDPSTLTIRAKE
                     TIQSLNQRLVRISDDVRRMAYQFHPSILDDLGLTAALKHMADEWSKKTGIKTVIVQEE
                     TTDPLPRDIASCLYRVAQESLTNIMKHARAARVELELTCGDQEITLSIYDTGVGFDLK
                     DIRARHPGLGLVNMRERVRSVQGRLDIQSQLGRGTHIIVHIPFSGAPHHEETTSSVS"
BASE COUNT          776 a          945 c          917 g          691 t
ORIGIN      
        1 cgcgcccgtg ccgacttgga tgtggcacaa gctcagattg cccgcaccaa ggccatcatc
       61 gccaagaaga ccatccgggc cccattccgt gcgcgcatcg gcatggcaga tgtccatccc
      121 ggccagtacc tggatgaagg gacattgctc acgacgcttc agggcgtcgg cgaagcggtg
      181 catgtggact tcaccgtcgc gcagcaggtc gccgcccgct tgcaggtcgg ggaaaccgtc
      241 gaagtgttcg cggccggtga tgcccccact gtcatagcca agatcgtggc cctcgacgcg
      301 cgtgtcgatc cgataacccg gaatgccatg atacgtgcca gaatcgaagg cacccgcaat
      361 gtaccggcac cgggagcttc ggtacgcgtc cggattccag tcggtcccga acatagtgtc
      421 atcgccatac cggttagtgc gttacgcaag gggccgggag gcgaccaagt attcgtcatc
      481 gcgccggaca aggacggccg caaccgagtc catacccgcc aggtagaaag cggccccatg
      541 ttcggcgatg agatcgttat ccaagccgga ctgacaaccg gtgaacagat cgccgcagtg
      601 ggctccttta agctccgcga cggtatcctt atcgcggtcg cggaatctgt cgagaataac
      661 tcaaaagaga atcacgaaac cgggaagcct taaaccgacc cacatgtcac agaacaatct
      721 ccgaacatcg ttcaccgacg tgagcctgag gagctgatgt tgacaagagc aagggcgtaa
      781 actatggtaa aactaccgcc gacaaaatcg tgattctctg tgtgctttcg tgagctgaga
      841 ccgcgaaggg agtaatctct cccgaaaaac caactcatct cctcatgcag cactttgatt
      901 gacctgaaac accggtcttg agaaaggaaa aggctatgca ccaccggttg tcacgcaagt
      961 ctttggacgc attgttggtt ctcgtgctcg ccctcaccgg ttgtactggg tggagcagtc
     1021 aaccgatcaa tgatccctca cgggacacct ggcagaaacc acaaggagtc gttgaaaagc
     1081 tgtccatcgc gcgaggatcg cgcattgcag acttgggggc cggcggaggc tattttacct
     1141 ggcatctggc caaggcggtc ggggcaagag gaacggtcta tgcggtcgat accgaggaaa
     1201 gagccattaa tacgatcttc caagaaatgg tctcccgagg gacgccgaat gtccgaccgg
     1261 tccgggccga gccgcgcgat ccaaaacttc ccgagcccgt cgacctggtg ttcagttgtg
     1321 atacctacca tcatatgatc gaccgcgtcg cctattttcg atctcttgcc tcgtcgctac
     1381 ggcccggcgg tcgcgtcgcg attctcgatc ttcatcctcg aggattcgtt tcggggctct
     1441 tcggtgacag agtcggaaag gaagaggtcc gacgcgaaat ggaggacgca ggctatctcc
     1501 tgatagacga tttcgatctt atcgaccgcc agcacttcca gattttcgcg aagtctgata
     1561 actgagatct cccggctcct ggctccccga tgaatcgttg agcttgacga accggatgtt
     1621 tctcgatgcc cctcgatcac cgcccaaccc atttgacgca agcagcccat cctcggtacc
     1681 ttacgtacaa ccgcaaccca tttgggggta acgggaagag ttatcatttc aatagttcat
     1741 cggcgacaag atgcgtatca atgctcaatt tagggaatct ctcctgatcg gcttcataat
     1801 cctgctcttc attgggatat ttatcttcga catctacacg cctacggcct tcaccgacca
     1861 tgcgctctat gtcatagtcg tgttgatcgc cacggcttca cgtttttcgt ggatgccccc
     1921 tctcgctgcc ggagcaggaa cactcctcac cgtggttgga gggcttggga ctccctggtt
     1981 taccgattta ccgcgatgga ttcagatggg aaaccggtcc atcacgatta tggtgttatg
     2041 ggtgctcgtc tggtttgcat ggaaacggag acaggcgaaa gcggccctac agaaggcgaa
     2101 tgaagagctc gagcaaaagg tcgcagaaag gacccaggaa ctcgccaccg tgaatcggac
     2161 cttggtcacg gaaatcgccg aacacgttca gaccgaacag gcgctgcgtc tctcgcaggg
     2221 acgacttgcc gacatcctgg acatcgccga agacgccatc atcgtcaccg agcatgatcg
     2281 atcgatcatg ctctttaatc aaggggcggt gaagctgttc ggctacgact cggatgaagt
     2341 gctggggaac cggatcgacc tgctgttacc cgaacggttt cggattgatc atccacccta
     2401 catcaacgcg ttcgcccata accctgaatc ggctcgttgg atggcgcaac ggcgtgaaat
     2461 gttcggcttg aaaaaggatg gaagcgagtt tccagcggag gcgagcatct caaagctgaa
     2521 ccttggcgaa aggacgacgt ttacggtgat cgtgcgggac atcacggacc gattgagaac
     2581 agaacggcaa ctccagtcgc tcaccgccga actgatgacg gcgcaagaag aagaacgccg
     2641 gcgcatcgca cgcgaacttc acgacgatgt gaatcagcgg ctggctttgc tggcgatcga
     2701 tatggcgaac atgttgtccg atccatcgac gctgaccatc cgagccaagg agacgatcca
     2761 atccctcaat caacggttgg tgaggatttc cgatgatgtc cgccgcatgg cctatcaatt
     2821 tcatccctcc atcctggacg acctgggtct gactgccgcg ctcaaacaca tggctgatga
     2881 atggtcgaag aagacgggga ttaaaaccgt catcgtacag gaagagacaa ccgatccttt
     2941 gccgcgcgat attgccagtt gtctctatcg ggtggcgcag gagagcctta ccaacatcat
     3001 gaaacatgcc cgcgccgcac gcgtggaact cgaattgacc tgcggcgacc aagagattac
     3061 gctatcgatt tatgataccg gtgtcggctt cgatctcaag gacattcggg ctcgccatcc
     3121 cgggttgggt cttgtcaata tgcgggagag ggtgcggtcc gtacaaggcc gattagacat
     3181 tcagtcccaa ctaggccgag gcacacacat cattgtgcac attccattct ctggagcgcc
     3241 acatcatgaa gaaaccacga gttctgttag ctgacgacca tacctttgtg ctcgagggat
     3301 tcaagaaatt gctggaagac cattgcgac
//
LOCUS       DATNDD010000160        11787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2007436, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000160 DATNDD010000000
VERSION     DATNDD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007436"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VN647_03780"
     CDS             <1..1175
                     /locus_tag="VN647_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWG96184.1"
                     /translation="LPDGPTLVVDIGGGSVELIVGNREGLIHCKSLKLGAIRLAEQFL
                     PKTPPSESMLQALDDAVLTHLREALGSFKMKKFHALVATSGMAGNLCEVVHLRQTGRP
                     LPQHNLAAVLSKDIRSLETELARSSVKARLAIPGLDPKRVDTLLPTAVILRRLLELSD
                     LSEVILCDKAIREGVIYDFIVRHREGLKAEHDIPDVRRRNVIGLARRCQAPEVHSLHV
                     ADLALSLFDQTKKEHRLGQQERTWLEYAAILHDVGYLINLRQHHKHAYYLIKHSDLGG
                     LTAEEIDVVANIARYHRRALPSLKHETFNCLTPRLQRVVKILASLLRIADGLDRTHFS
                     LVRVVNVKLGKQMTIEVRLTGDAEMELWAAKSRADLFEQVFRRRVLFSGVLETEPS"
     gene            1172..1921
                     /gene="tmk"
                     /locus_tag="VN647_03785"
     CDS             1172..1921
                     /gene="tmk"
                     /locus_tag="VN647_03785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWG96185.1"
                     /translation="MTEPHILKATPHPHPGKLIIVEGIDGSGKSTQLQLLHKWLESKG
                     HKVFFTEWNSSALVKETTKRGKKSKSLTPTTFSLLHPTDFASRLYHQILPPLKAGMLV
                     LADRYMYTAFARDVVRGVANEWVRKLYAFSIKPDMAFYFKVPIEVAISRLSRGTRGHF
                     KYYEAGMDMDLSPDITESFRLFQSRILAEYDKIVDECGLLTMDATQDIETQQEHMRAL
                     VEEALHGYKPRRGTYGRRTLFWRRFEVSRSE"
     gene            1869..2591
                     /locus_tag="VN647_03790"
     CDS             1869..2591
                     /locus_tag="VN647_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWG96186.1"
                     /translation="MADARFFGDGLKYLDPSELKGKLIAIEGTDGVGRSTHIEMLQEW
                     LEVQGYGVMTTGWTRSNLMSKTIEMAKAGNIVDRWSLSLLYKTDFADRLEHQIIPALR
                     SGFVVLTDRYIYTAFARDFVRSADRTWIRDVFGFALIPDLVCYLRIDVETLALRVIET
                     TGMNYWESGMDLRLGGDLYDSFKKYQSLLIEEFDKMAIEFRFDVVDARKSPEEIQDEL
                     RGHILPVLQNNKPTVGTETASS"
     gene            complement(2588..3121)
                     /locus_tag="VN647_03795"
     CDS             complement(2588..3121)
                     /locus_tag="VN647_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWG96187.1"
                     /translation="MDCILVRHGIAVEPDEWEGTEENRPLTEKGKRRARQAAEGLAAL
                     DCKPTHLFTSPFVRAYDTARLLRAVVCPTLKVETREELVVGAKPEQVVALLHTLPSDS
                     AVVCVGHEPLLGEVVSLLLCGKALPNFPLKKAGAACIHADEVIRPGHGRLRWWLQPMQ
                     LRTLGKRAQNKKEVKRV"
     gene            complement(3163..3558)
                     /locus_tag="VN647_03800"
     CDS             complement(3163..3558)
                     /locus_tag="VN647_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWG96188.1"
                     /translation="MATLVRDIMKGLSPTRRKKIETRASELIAEEMSLKDLRQALTLT
                     QQRIAKTLGIGQEGVSRLEQRSDLLLSTLRSYIEAMGGQLSLIAEFPDREPVALSGLC
                     AMETTPRRTPRPSRRRRIRPHADQSKGVY"
     gene            complement(3561..3941)
                     /locus_tag="VN647_03805"
     CDS             complement(3561..3941)
                     /locus_tag="VN647_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWG96189.1"
                     /translation="MSYSPGENWEVEIGDEFRPEFHELPEDVQDEILAHARLIEELGP
                     ELGRPRVDTLKGSRYANMKELRFHAADGVWRVAFAFDPRRHAILLVAGDKSGGSERRF
                     YEALIRKADKRFYDHLGRLKQKRK"
     gene            complement(4137..4448)
                     /locus_tag="VN647_03810"
     CDS             complement(4137..4448)
                     /locus_tag="VN647_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96190.1"
                     /translation="MFTSELSLAVKAYAQIGLCYKLSGHCEEAVTAFQKALKATPASS
                     RETVQILYVLGRTMESLGRVAETLEAYRWIRREDASYRDVAERIERLSSRRPSLAMKK
                     N"
     gene            complement(4459..5865)
                     /locus_tag="VN647_03815"
     CDS             complement(4459..5865)
                     /locus_tag="VN647_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWG96191.1"
                     /translation="MYEAFYHLKTKPFALLPDSSFLYSGSEHQTAYSLLEYGILSQAP
                     FMVLTGEPGVGKTSLLQKLIAEHGNRHKIGLVTHAHYDIEHLLPWILLSLGLSTKRLD
                     PIEAYHMFSAFLAQESKSLRRVILVIDEAQNLGAERLEELRLLSNMNDGKILKLQIIL
                     SGQPDLYTLLQRIDMTQFAQRIVVDYHLKPLTEVDTARYIRHRIRVAGGHPALFTNKA
                     CVLVHRLSQGNPRLINQVSDRALIYGYAEQAKVVTSKLVAQAALDRTKGGILPLAAKD
                     ELVALATDTEDMAEINDPLPSLETEVHADESFGSRPPDSPEEAYAHGMALKDQGRLKE
                     AVDMFHLAAQGKSMRLKAHAQVGLCYRRMREHQAAIQAFRSALNDQSAPPNDIIDVLY
                     FLGQSLEFTGQTEEALEVYRRVIQLNPGFANVADRVRGLRQSLEQTSLEKEPGMSNRS
                     WLGGVITKLWRSLTGSQK"
     gene            complement(5890..6717)
                     /locus_tag="VN647_03820"
     CDS             complement(5890..6717)
                     /locus_tag="VN647_03820"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HWG96192.1"
                     /translation="MDRIRTALELYKESEGTSSDRVSAKRAAEHSLTSSITYTRTRSL
                     TVPLPVLREHRVMAANKYGPFIDAYKILRTQVTQRLRENGWNVVGVTSPGYGEGKTLT
                     AVNLAVSLAMETTQTVLLVDSDLQDPTVHQVFGLQNCLGLADYLLDDQPVEDLLIHPG
                     IGRFVLLPGGRALSNSTEILTSPKMLALVEELKHRYPSRIVVFDLPPLLHTADVLAFS
                     PYTDALLMVVEEGKTTGEELQRALRLVKGSRPVLGTVLNKAGRSSLTIAEMKTRLAP"
     gene            complement(6731..8461)
                     /locus_tag="VN647_03825"
     CDS             complement(6731..8461)
                     /locus_tag="VN647_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWG96193.1"
                     /translation="MSLKDYIAAFHRRRKLILLTGLGFLTISLTLAFLWPPTYKSTAT
                     ILIEEQEIPSELVRSTITSYADQRIETIKQQVMSRTTLWKVVEQFGLYHDQQQNSPAE
                     EIVKRFIKDVEVEVISADVVDKRTQHATKATIAFTVAYQNRSPELAQKVANELTSLFL
                     GENLKSRERQAQETTSFLQQEAENLARHISEIDEKIASFKHRANGALPELMSLNQQMM
                     NQAERELMDVDQQVRNLEERKAYLEGELATIKPNTPILSVTGERILDSVERLRALRAE
                     YVGAAANRSSDHPDIFKMKQEIEALEKETGQLSDADETSKRLVDARATLAADLERLGD
                     DHPDVLQIRRKIAALEQEVVRLNAVPERKITPRPENPAYINLQAQLNSATSSLEAFRK
                     TRLDIKRRLQVYATRLEKGPEIEPEYLVLTRDRDTSGQKYQDIRSRLLEAKVSEGLEV
                     QRKGERFSLIDPPSLPEKPFKPNRMAIIFLGFILAVGGGIGSGAVAESLDHSIRTPDQ
                     LAALTQQFPLAVIPFMPNEKDLFRAKMRWRLVKTAGVGVVAVTLVILHVFVIPLDVLW
                     FAALRRFGVG"
     gene            complement(8515..9651)
                     /locus_tag="VN647_03830"
     CDS             complement(8515..9651)
                     /locus_tag="VN647_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96194.1"
                     /translation="MTFHSEAAEWSLSPSVGAKAVYNSNLLLTPQPHTETYGFWVSPA
                     TEIAGKTERLEVSSRLAADVVRYFGGQDREFTNVFAPLSIRYKTEKDLLGFTGGFVRD
                     NTLLGAELQETGVVLQFVQRNQWTFNPTWTRRLTEKLSLQSSIQFSHTTYESDRLVGY
                     RVVGGSTGFLYQLSERDQFQLTGSYVDFQTANSVFPVRASYPGINMSFTHAFSESLTG
                     TVYGGPRFLSSSSQTTLGDITASETEWLGGASIAKRFERASLQVSFSRDLTPSGFGLL
                     LETDRGALSASYDISETLACSLNVNGILTSGKTNAVIGGAFPDRRYVSVTPTLSWKFL
                     EWWQAEVSYMYRWREIDTDVSSSAQSHAVTFMVTYYPPNFSWSY"
     gene            complement(9751..10353)
                     /locus_tag="VN647_03835"
     CDS             complement(9751..10353)
                     /locus_tag="VN647_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWG96195.1"
                     /translation="MFCSARLVMSALFMVIVVTVADLSVYAEDGLTAALESKIDPGYR
                     LGPEDVMLVSVWKDEQLTREVVVRPDGMFSFPLVGDIRAEDRTVEDISADLVRRLTKY
                     IPNPNVSVAVTKVVSYKVYVIGRVNKPGEYLIGHYTDVLQVLSLAGGLTPFAAENDIK
                     VMRRVRGEQHAIPFRYGDVRKGRDLEQNIILQRGDVVMVP"
     gene            complement(10363..>11787)
                     /locus_tag="VN647_03840"
     CDS             complement(10363..>11787)
                     /locus_tag="VN647_03840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96196.1"
                     /translation="YDILVEEYKGKPVGLEAKVKLAEMDLLTGKQADAERQVEEVLKQ
                     NPRSSDGLILSGRMALARRNGKDAVQAFRIVLHDQPNMAMVHYLLGQAYQLTGEINLA
                     KESFEQAVALYPDQVEAKRSLAVLDSKSGRYEQAHARLDDLLKRRPDDIAALDMLMML
                     DLVTKNWPEAERTLQRLQAVAKESLLTHMAEGRLHQAQGRFERAVAAYERAAISAPTD
                     PDPLLSLVKLEVAQGHLEQVKLRMEAMIAKRPDHVFAHGLLGEVSMMAGDYSGADAHF
                     DQATKLNPKWLTPWLNWGSLWLGQKRFDQAVLVVKNGLRANAESEELHMLLASVHSAQ
                     GKVDDVISAYDAALRLNPKNVLVANNLAVLLVDHKGDPQSLQKAFALSRDFEKETPHP
                     LFLDTLGWVRFKMGQQEEAIRLLKYAVAKSPDTSILNYHLGMAFFQSGKSAEARPYLS
                     KALKHREQFEGRQEAEQVLARIRR"
BASE COUNT         2695 a         3343 c         3025 g         2724 t
ORIGIN      
        1 ccctcccgga cggaccgacc ctggtcgtcg acatcggggg cggttccgtg gagttgatcg
       61 tggggaatcg agaaggcctg atccactgca agagcctcaa gcttggagcg atccgtttag
      121 ccgagcaatt ccttcctaaa actccaccgt ctgagtcgat gctgcaggcc ctggacgatg
      181 ccgtgctcac ccacctgcgc gaggcgctcg gatcattcaa gatgaagaag ttccatgcat
      241 tggtcgctac atccggtatg gcaggaaacc tgtgcgaggt cgtccatctc cgtcaaaccg
      301 gacgaccgtt gccgcagcac aatcttgcgg ccgttttatc gaaggatatc cgcagcctcg
      361 aaacggagtt ggctcggtca tcggtgaagg ctcgtttggc gattcccggt ctggacccca
      421 aacgtgtcga tacgctgttg ccgacagccg tgattctccg gcgtcttttg gagctatccg
      481 atctcagcga ggtcatactc tgcgacaagg ctattcgtga gggtgtaatc tacgatttta
      541 tcgtacgaca tcgagagggt ctgaaagccg aacatgatat tcccgacgtt cgacgtcgca
      601 acgtgatcgg cctagcccga cgctgccagg caccggaggt tcattcactc catgtggccg
      661 acttggcact gagtttgttc gatcaaacca agaaagaaca tcgcttggga caacaggagc
      721 gaacctggct ggagtatgca gccattctgc acgatgtcgg atatctcatc aatctgagac
      781 aacatcacaa acacgcgtac tacctcatca agcatagtga cttgggcgga ttgacggctg
      841 aagaaatcga tgtggtcgcc aacatcgccc gctatcaccg gagagcgttg ccgtcgctga
      901 agcacgaaac attcaactgt ctgacaccac gtttgcagcg tgtggtcaag atccttgcct
      961 cattgctgcg gatcgccgac gggcttgatc ggacccactt ctcgctcgtt cgggtcgtga
     1021 acgtcaagtt gggaaaacag atgaccattg aagttcgttt gacgggggac gctgagatgg
     1081 aattatgggc tgcaaagagc agagccgacc tgttcgaaca agttttccgt cgacgtgtcc
     1141 tattctccgg agtgctggaa accgagccat catgaccgaa cctcacatac tcaaagcaac
     1201 ccctcaccct cacccgggga agctgatcat cgtcgaaggc atcgatgggt caggcaagag
     1261 tactcaattg caactgctgc ataaatggct tgagtccaag ggacataagg tgttctttac
     1321 cgaatggaat tcctcggcac tcgtgaaaga aaccaccaaa cgagggaaaa aatcgaagag
     1381 cctcactccg acaacgttca gtttgctgca tcccacggat tttgccagcc ggctctatca
     1441 tcagattctc ccgcccctga aggcgggcat gcttgtcctc gccgatcgat atatgtatac
     1501 ggcgtttgcg cgcgatgtgg tgcgaggcgt ggcgaacgag tgggtgcgga aactctacgc
     1561 gttttcgatc aaacccgata tggcgtttta tttcaaagtc ccgatcgaag tcgccatctc
     1621 ccggctctcg cgagggactc gcggccattt caaatactat gaagcgggca tggacatgga
     1681 tctgagtccg gacatcaccg aaagcttccg gctctttcaa tcacggattc tcgcggaata
     1741 cgacaagatc gtcgatgaat gcggtctctt gacgatggat gcgacccaag acatcgagac
     1801 gcaacaggaa cacatgcggg cgctggtgga agaagcactc catggctaca aacccagacg
     1861 aggaacctat ggccgacgca cgcttttttg gcgacggttt gaagtatcta gatccgagtg
     1921 agctgaaagg caagttgatc gccatcgagg gaaccgacgg agtcggccga tcgacccata
     1981 tcgagatgct gcaagagtgg ctggaggtcc aggggtacgg cgtgatgacg acggggtgga
     2041 cccgttccaa tctcatgtcc aaaaccatcg aaatggcgaa ggccggcaat atcgtcgatc
     2101 gttggtcgct cagtctcctc tataagacgg atttcgccga tcgcctcgag catcagatca
     2161 ttccggctct ccgatccgga ttcgtcgtgc tgacggatcg ttacatctac acggcgttcg
     2221 cgcgcgactt tgtgcggagc gccgaccgaa catggatccg tgacgtgttt gggtttgcat
     2281 tgatccccga cttggtctgc tacttacgaa tcgatgtgga aaccctggcg cttcgagtca
     2341 tcgagaccac cggaatgaat tactgggaat ccggcatgga tctccgcctc ggaggcgacc
     2401 tctatgatag tttcaaaaaa taccaatcgt tgttgatcga agaattcgat aagatggcga
     2461 ttgaatttcg cttcgatgtc gtcgatgcga gaaagtcgcc cgaagaaatc caagacgagc
     2521 tccgaggaca tatcctcccg gtactacaga acaacaagcc tacggtcggc acagagacag
     2581 cctcttctta aacccgtttg acctctttct tgttctgagc gcgcttccct aaagtccgga
     2641 gctgcatcgg ctgaagccac cagcgcagcc gcccatgacc gggtctgata acctcgtccg
     2701 catgaataca cgcggctccg gccttcttaa gcggaaaatt gggaagggct tttccgcaaa
     2761 gcaataaact cacgacttca cccaacagcg gctcatgtcc cacacacaca accgctgagt
     2821 ctgacgggag cgtatgaaga agcgctacca cttgttcggg tttcgctccg accaccagct
     2881 cctcgcgtgt ttcgaccttc aaagtcggac agacgaccgc acgcagtagt cttgccgtat
     2941 cataggcccg cacgaatgga ctcgtgaata gatgggtcgg cttacagtca agtgcggcca
     3001 acccttcagc agcctgccgc gcgcgccttt tccctttctc cgtgagagga cgattctctt
     3061 ccgtcccttc ccactcatca ggctctacgg cgattccatg acgaaccaga atacagtcca
     3121 taatcacctc tctacatagt gagactcgtg cagaacaggg agtcagtaga cgcccttaga
     3181 ttgatcggca tggggtcgta tgcgccttct gcggctgggg cgcggggtac gccgaggagt
     3241 cgtttccatg gcacatagcc ctgagagggc gacgggctcg cggtcaggaa attcggcgat
     3301 cagtgagagt tgaccaccca tggcttcaat gtagcttcgc agagtggaca acagcaaatc
     3361 gctgcgttgt tcgagacgcg atacaccttc ctggccaatg ccaagggtct tggctatacg
     3421 ttgctgcgtg agagttaagg cctgccgtaa atcctttagg gacatttcct ctgcgatcag
     3481 ctcgctggcg cgcgtctcaa tctttttgcg gcgggtgggg ctcaagccct tcatgatgtc
     3541 tcgtacaagg gttgccacgg ctatttcctt ttctgtttca atcgcccgag atggtcatag
     3601 aagcgtttgt cggccttccg gatgagtgct tcgtagaaac gtctttcgct tccccctgat
     3661 ttatccccgg cgacaagcag gatggcatgc cgtcggggat caaacgcgaa agcgacacgc
     3721 cagactccat cagcggcatg aaagcgaagt tccttcatgt tcgcgtagcg cgaccccttg
     3781 agtgtgtcca cacggggacg acccagttca gggcctaatt cttcgatcag gcgcgcatga
     3841 gccagaattt cgtcctggac gtcttcaggc aactcatgga actccggccg aaattcatcg
     3901 ccgatctcaa cctcccagtt ttcacctgga gagtatgaca tacaatacat atgctgtcaa
     3961 atacatgttt tgcagacggt caggtcttgc catcatacat ttcctgctcc atctgattga
     4021 gcccgacgct caccgtcgca tagtgaacgc ctaatagggc ggcggaaggt cggccgaaag
     4081 gcatctcaga ggatgagaag cgcgccgcca tagctgtaga aaggccgatt ccggaatcag
     4141 ttctttttca tagccagaga ggggcggcgg gagctcagcc gctcaatacg ttcagcgaca
     4201 tcccgatagg acgcatcctc ccgcctgatc cagcgatagg cttccagcgt ttccgctacc
     4261 cgacccaatg attccatcgt gcgcccgagg acatagagaa tctgaactgt ttctcttgag
     4321 gacgccggcg tggccttgag ggctttctga aacgccgtaa cggcctcttc acagtgaccg
     4381 gacaatttat aacaaagtcc gatctgagca taggccttga cggccaagga tagctccgag
     4441 gtgaacatac agggctgtct acttctgact gccggtgaga gaacgccaca atttcgtgat
     4501 caccccgcca agccatgaac ggttcgacat acccggctcc ttctcgaggc ttgtctgttc
     4561 caggctctgc cgcaatcccc ttactcgatc ggcgacattt gcgaaccctg gattcaactg
     4621 aatgacgcga cgatagactt ccagggcttc ctcggtctgc ccggtaaatt ccagactttg
     4681 cccaaggaaa tacaagacat caatgatgtc gttcggtggg gcagactgat cattcagcgc
     4741 gctgcgaaat gcttggatag ccgcctgatg ctccctcatt cttctatagc agagaccgac
     4801 ctgagcatga gccttcagcc gcatcgactt gccctgcgcc gcaagatgga acatgtcgac
     4861 agcctctttt aaccgtccct gatccttgag agccattcca tgggcgtacg cttcctctgg
     4921 agaatccggg gggcgagagc caaatgattc atcagcgtgg acttccgtct cgagactagg
     4981 aagagggtcg tttatctcgg ccatatcctc agtatccgtc gccagtgcaa ccagctcgtc
     5041 tttggcggcc agaggaagga tccccccctt cgttcgatca agcgccgctt gggccaccaa
     5101 cttggaggtg acgacttttg cttgctcggc atacccatag atcaatgctc tgtcggacac
     5161 ttgattgata aggcgcggat tcccctggct cagacgatgc accagcacac aagccttatt
     5221 ggtaaagagg gctggatgtc caccggcgac tcgtattcga tgacgaatat atcgcgcggt
     5281 gtcgacctcc gtcagtggct tgagatgata atcgacgaca atccgctgag cgaattgagt
     5341 catgtcgata cgctgcaaca gcgtatacag atccggctgt ccggaaagga tgatctgcaa
     5401 ctttaagatc ttgccgtcgt tcatgttgga gaggagccgg agctcttcga gccgttcggc
     5461 acccagattc tgagcctcgt caatgaccag gatgacccga cgtaggctct tcgattcctg
     5521 agccaggaat gccgagaaca tgtgataggc ctcgatcgga tcaagccgct tggtgctcaa
     5581 tcctagagac aacaaaatcc aaggtaacag atgttcgata tcgtagtgcg catgtgtgac
     5641 cagaccaatc ttgtgtctgt tgccgtgttc tgcaatcaac ttctgcagaa gggacgtttt
     5701 tccgacacca ggctcacccg tcagaaccat aaacggcgcc tgactcagga tgccgtactc
     5761 gagtaaactg taagcagtct ggtgttctga accggagtac agaaaactac tatccggcaa
     5821 aagggcgaac ggcttggttt tcaagtgata gaatgcttcg tacatgggtg cgcacttatt
     5881 tttttcgcgc tagggggcta acctcgtctt catctccgca atggtcaggg acgatctccc
     5941 ggccttgttc agcactgttc caagaacagg gcgggaaccc ttgactaacc tcaacgcccg
     6001 ctgtagctcc tcaccggtcg tctttccctc ctcgaccacc atgaggagcg catccgtgta
     6061 aggagagaaa gccaatacat ccgccgtatg aagcaacggg ggaagatcga acacgacaat
     6121 ccgagagggg taccgatgtt tcaactcttc aaccaaggct aacatcttcg gcgacgtcaa
     6181 aatctcagtg gagttcgaga gagcccgtcc ccccggcaac agcacgaatc gccctattcc
     6241 cggatggata aggaggtcct cgacgggctg gtcgtccagc aagtagtccg caagcccaag
     6301 acaattctgt aaaccgaata cttggtgcac agtggggtcc tgaagatccg agtcgaccag
     6361 caacaccgtt tgcgtggttt ccatcgccag gctcaccgct aaattcacgg ccgtcaatgt
     6421 cttcccttct ccgtagcccg ggcttgtcac ccccaccaca ttccacccgt tctcccgcag
     6481 tcgctgcgtg acctgcgtcc gtagaatctt ataggcatcg atgaacggtc cgtacttgtt
     6541 cgcggccatg acccgatgct cacgcagaac gggaagcggg acggtgagcg acctcgttcg
     6601 tgtgtaggtg atggaagacg tgagcgagtg ctccgcagcc cgtttcgcgc tgactctatc
     6661 ggaagacgtg ccctccgact ctttatacag ttcaagtgcg gtccgtattc ggtccatggt
     6721 gttccttggc ctaccctacg ccgaaacgtc tcaatgcggc aaaccacaac acatccaaag
     6781 gaatgacaaa gacgtgcaat atgacgagcg tcactgccac gacgccgact cccgcggtct
     6841 tgacgaggcg ccatcgcatt ttcgcccgaa agagatcttt ttcattcggc ataaaaggaa
     6901 tcacagccaa aggaaactgc tgcgtcaggg cagccaactg gtcgggtgtt ctgattgaat
     6961 gatcgagtga ttccgccacg gctcctgagc cgattccgcc accgacagca aggataaacc
     7021 ctaggaagat gatggccatc cgattgggtt tgaacggttt ttcagggagg ctcggcgggt
     7081 caattaacga aaagcgttcg cctttccgct gcacttccaa tccttccgac accttcgcct
     7141 ccaacaacct cgaccgaatg tcctgatatt tttgccccga cgtatcgcga tctctcgtca
     7201 aaaccaggta ctccggctcg atctccggtc ccttttccag acgcgtcgca tacacttgca
     7261 gccgacgctt gatgtcaaga cgggtctttc gaaatgcctc caacgaagaa gtcgcagaat
     7321 tcagctgagc ttggagattg atataggcgg ggttttccgg gcgcggagtg atttttctct
     7381 caggcacggc gttgagccga acgacttcct gttcaagcgc cgcaattttt ctccgaatct
     7441 gaaggacgtc cggatgatca tcccccagac gctccaaatc agccgccaat gtggctcgag
     7501 catctaccag acgcttcgag gtctcatccg catcggagag ttgaccggtc tccttctcca
     7561 aggcttcgat ttcctgtttc atcttgaaaa tatcaggatg atcagaagaa cgattggctg
     7621 ccgctcccac atattccgca cgaagcgccc gcaaacgctc aacagaatcc aaaatgcgct
     7681 cgcccgtgac ggacaaaatc ggcgtattcg gcttgatcgt cgccaattcc ccttcgagat
     7741 aggccttgcg ctcttcaagg tttcggacct gctgatcgac gtccatcaat tcacgttccg
     7801 cttggttcat catctgttga ttcagcgaca tcaactccgg aagcgcccca ttggctcgat
     7861 gtttgaacga tgcgatcttt tcgtcgattt cgctgatgtg ccgagcaaga ttttccgctt
     7921 cttgctggag gaacgaggtg gtttcctgag cttgacgttc gcggcttttc aggttttccc
     7981 ccaaaaacaa gctggtcagt tcgttcgcca ctttctgtgc cagttccgga gagcggtttt
     8041 gataggccac cgtaaaggca atggtcgcct tggtcgcatg ctgtgtgcgt ttatccacga
     8101 cgtcggcact gatcacttcc acctcgacgt ccttgatgaa ccgtttgacg atttcttcgg
     8161 ccgggctgtt ctgctgctga tcgtggtaga gaccgaactg ctcgaccact ttccagaggg
     8221 tcgtgcggct catgacttgt tgtttgatgg tctctattcg ctggtcggca taactcgtaa
     8281 tagtggaccg tacgagctcg gatggaattt cctgctcttc aatcaatatg gtggcagtcg
     8341 acttataggt cggtggccag agaaacgcca gcgtcaaaga tatcgtcaag aatcccaagc
     8401 ctgtcaggag gatgagtttg cgacgtcgat gaaaggctgc aatgtagtcc ttcaaactca
     8461 tgggtcgctc ggcggattgt atcgaagtat gcgtctgtgc catagcttca agcttcagta
     8521 ggaccatgaa aaatttggtg gataataggt gaccatgaac gtcaccgcat gagattgggc
     8581 tgatgagctc acatcagtgt cgatctctcg ccatcgatac atgtacgata cctccgcctg
     8641 ccaccattcg agaaacttcc aggagagcgt cggcgtgaca ctgacataac gtctatccgg
     8701 aaaggctccc cctattactg cgttggtctt tccagacgtg aggatcccgt tgacatttaa
     8761 ggagcaggca agggtttccg atatatcgta tgacgcggaa agtgctcctc gatcggtctc
     8821 aaggagtaat ccgaacccgc ttggagtaag atcccgagaa aatgagactt gcaatgaagc
     8881 tctctcgaac ctcttcgcta tacttgctcc tcctagccac tccgtctcgc tagccgtgat
     8941 gtcgccgagt gtggtttgcg agctcgacga taagaatctg ggaccgccat agacggttcc
     9001 tgtcagcgat tcagaaaagg catgtgtgaa actcatattt attccaggat agctcgctct
     9061 aacaggaaat acggaattgg ctgtctgaaa atcaacgtac gatcccgtga gctggaactg
     9121 atcccgttca gaaagttgat acaggaatcc agtggaacct cccaccactc gatagccgac
     9181 caatctgtca ctctcatatg tggtgtggga aaactgtatg ctcgattgca atgacagctt
     9241 ttcggtcaac cttcttgtcc acgtagggtt aaatgtccac tggttgcgct gcacaaactg
     9301 cagaaccact ccggtttcct gcaactccgc accgagtagc gtattgtcac ggacaaagcc
     9361 gccggtgaag ccgaggagat ccttctcggt cttataccgt atggacaacg gagcaaagac
     9421 atttgtgaac tccctgtcct gtccgccgaa atatcttaca acatccgcag cgagccggct
     9481 gctcacctca aggcgttctg tcttgccggc gatctctgtc gctggagaaa cccaaaagcc
     9541 gtacgtctcg gtgtgaggct gaggagtcaa gagcaagtta ctgttataaa ccgccttagc
     9601 cccgacggat ggcgagagcg accattctgc cgcctcgctg tggaatgtca tgctggactc
     9661 cgtgaggcag acccacaaga tgcatcgccc aagccatcgt acgtgcttcg atcgctcaca
     9721 acctccaacc tcacgcctcc aatgcatttc ttacggtacc atgacaacgt caccgcgctg
     9781 gagaatgata ttctgctcca aatccctacc cttccgaaca tcgccatagc gaaagggaat
     9841 ggcatgttgt tctcccctca ctcgccgcat gaccttgata tcgttttccg cggcaaatgg
     9901 agtcagcccc ccggcaaggc tgagtacctg caacacatcg gtataatggc cgatcaagta
     9961 ttcgcccggt ttattcactc gcccaatgac atacacttta taactcacta ctttcgtgac
    10021 tgcgaccgac acattcggat tcgggatgta cttcgtcaat cgcctgacga gatcggcact
    10081 tatgtcctcg actgttcgat cttcggcccg gatatcaccg acgagaggaa aagaaaacat
    10141 cccatccgga cggacgacga cttctcgcgt cagctgttca tccttccaga cagaaaccag
    10201 catgacatcc tcgggaccaa ggcgataccc cggatcgatc ttggattcca gtgcggcagt
    10261 caaaccatcc tcggcataaa cagacaggtc agctaccgtg acgactatca ccataaacaa
    10321 ggcgctcatc accaatcgag cagaacaaaa catgaacact ccttaccttc tgatccgtgc
    10381 taagacctgc tccgcctcct gccgaccttc aaactgttca cggtgcttca atgcttttga
    10441 aaggtacgga cgggcttccg ctgatttgcc tgattgaaag aatgccatcc caagatgata
    10501 attcaggatc gaagtgtccg gggatttggc aaccgcatac ttcaataagc gaatggcttc
    10561 ttcttgctgt cccattttga atcgaaccca gccgagggtg tcgaggaaca gagggtgcgg
    10621 agtctctttc tcgaagtctc gactcaatgc aaatgccttt tgaagacttt gtggatcgcc
    10681 cttatgatcc accagtagaa ccgcgaggtt gttggcgaca agtacgttct ttgggttcag
    10741 ccgcaatgcc gcgtcgtaag cgcttatcac gtcgtcgacc ttcccttgag ccgaatggac
    10801 tgaagcaagg agcatatgca actcttcact ctcagcatta gcccttagcc cgtttttcac
    10861 gaccaaaacc gcttgatcga accgtttctg gccaagccac agacttcccc aattcaacca
    10921 tggcgtcaac cacttcggat tgagtttcgt ggcctgatca aagtgggcat cggcgccgga
    10981 gtagtcaccg gccatcatag acacttcacc caataaacca tgggcgaaca cgtgatccgg
    11041 tcgcttcgcg atcatggctt ccatccggag cttcacttgc tcaagatgtc cttgagcaac
    11101 ttccaacttc accaaagaca ggagaggatc aggatcggtc ggcgccgaga ttgccgcacg
    11161 ctcgtaggcg gcaacggctc gctcaaatcg accttgcgcc tgatggagtc gcccctccgc
    11221 catatgggtg agcagactct cttttgctac agcctgcagg cgctgaagag tgcgctcggc
    11281 ctctggccaa ttcttcgtca ccagatccaa catcatcagc atgtcgaggg ccgctatgtc
    11341 atcggggcgc cgttttagga ggtcatcgag tcgtgcgtgc gcctgttcat aacggccgct
    11401 tttgctgtcc aacactgcaa gcgatcgttt agcctccacc tggtcgggat agagagccac
    11461 agcttgctca aagctttcct tcgcaagatt gatttcccca gtgagctgat aggcctgacc
    11521 aaggagatag tgaaccatcg ccatattggg ttgatcgtgc aatacgatac gaaaggcctg
    11581 caccgcgtcc tttccatttc gtctggccaa ggccattcgc cctgaaagaa tcaaaccatc
    11641 ggaagagcga gggttctgtt ttaacacctc ttctacctgc cgctcagcgt cggcctgttt
    11701 ccctgttagc aaatccattt ccgccaattt gaccttggcc tccagaccga ctggtttgcc
    11761 cttgtactct tctaccaata tgtcata
//
LOCUS       DATNDD010000161         5059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2397615, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000161 DATNDD010000000
VERSION     DATNDD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397615"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /gene="yidC"
                     /locus_tag="VN647_03845"
     CDS             complement(<1..1486)
                     /gene="yidC"
                     /locus_tag="VN647_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWG96197.1"
                     /translation="MEKRVIVFLVLSLAIIFGYEFLLKELGLLPQPTISEPADPSTSD
                     TTFPSPSSTSPGAPTSSAPETKETVPLKPSAEDRRTQEPLGEPSTMKVVEVETDLYRA
                     KFTTRGAALISWELKRYQNSSDGKSAVQLVRQGGKFAEPLTITADDAAHSKELSEGIY
                     RVEKDFTTLDQGHPTGHMTFYYANPATGVLLEKQLTFHADSYVVDIALKPSGLGGVKV
                     GLGTNFGVVDWGEGFIGSVGAASLIGDKIENDMPESETERKGALKWVALQDKYFIAAL
                     IPKTNASAFPRKQGDKLVSTAVRLAVDSSGAPLGLQLFAGPKEFDTLQSLQIRLEDTI
                     DFGWFLFGSWDTVRAVAKPIFYVLRYINEYTHNYGLTIILLTVIIKLLFVPLQYKSYT
                     SMKKMQGIQPKVAAVQEKFKDDRERLNKELIKLYRDHKVNPVGGCLPMVLQMPVFVSL
                     FNILYMTIDLRQAPLGLWITDLSVQDPYYILPIIMGISMVVMQKI"
     gene            complement(1523..1801)
                     /gene="yidD"
                     /locus_tag="VN647_03850"
     CDS             complement(1523..1801)
                     /gene="yidD"
                     /locus_tag="VN647_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWG96198.1"
                     /translation="MSWSKRGGARLSTCTFFDQGHIDMQWLCLRLIRGYRLVISPLYG
                     RTCRFEPSCSQYASDAIIKYGTLQGIGLAILRLMKCHPFHPGGEDPVK"
     gene            complement(2243..2380)
                     /gene="rpmH"
                     /locus_tag="VN647_03855"
     CDS             complement(2243..2380)
                     /gene="rpmH"
                     /locus_tag="VN647_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWG96199.1"
                     /translation="MSFTFQQPSNLKKKREHGFRKRMSTKNGRKVLARRRAKGRARLT
                     V"
     gene            2854..3465
                     /locus_tag="VN647_03860"
     CDS             2854..3465
                     /locus_tag="VN647_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96200.1"
                     /translation="MLRKASLVSLSLTLVVMAGCAEPPTQQIQEAEKALKDAQESGAA
                     TYSADEYAKLEGTLSAIRKEVADQDGKFTLFRDYSKAQQLSASAKADSERIKAASSQK
                     KEEAKAAALQAQQVAEEAVKATQDLVAKAPVGKDRAAVEAIKNDVEGLKSLLKQVQVS
                     IDKEDYPAAQTQAKAIHDMSQGVSTEIQSALAKVGKGKLARKK"
     gene            3474..4607
                     /locus_tag="VN647_03865"
     CDS             3474..4607
                     /locus_tag="VN647_03865"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWG96201.1"
                     /translation="MALDQTHKTLAVILCVLCLTGCVQAVPPDLLVALEAIDQDLIAL
                     RAPEAAPAEYGQFVHQWVSLKARVQLDDDLIRWPWESNDLENELRRLYIMGERTVSQL
                     HERQASQHRVAQAKVVRLEERLHTITSRVESIDGRILLGDKVVQTDLLVKQARSFFEQ
                     KDFQRAMQAAEKADHALKAQASLLSQELGRYADERRIAYWQTLAKQTIAWSRIHQSTA
                     IVVSKADRELTLYKSGRKVLSYPVRLGFNGMLEKQFQGDGATPEGRYRVTDKRGQGQT
                     QFYRALALDYPNAEDRRRFELAKRSGRIGPAKGIGGQIEIHGADNELLAQTLGCIMLD
                     NPNMAVLYEGVFVGTPVTIVGALTRENAVALALSELAQRKDKI"
     gene            4618..>5059
                     /locus_tag="VN647_03870"
     CDS             4618..>5059
                     /locus_tag="VN647_03870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWG96202.1"
                     /translation="MRRIHLAAAAITLLLLLIVSALLVVSDPTPSRVASEDSQVVHQP
                     AAQDKAGLRTLQARYKVLSKQLSQLMPNQPYILVDTARNRLYVKHQEQVVLDAIASTG
                     SGTILDKPGEGHSQWIFDTPRGEFLVKSKLTNPTWIKPDWAFVEE"
BASE COUNT         1265 a         1305 c         1339 g         1150 t
ORIGIN      
        1 ggatcttttg catgaccacc atgctgattc ccatgatgat cggcagaatg tagtagggat
       61 cctgaactga cagatcggta atccacaatc ccaacggtgc ttgacgtaaa tcgatcgtca
      121 tatacagaat attgaacagc gatacgaaga ccggcatctg caataccata gggagacagc
      181 cgccgacggg attgaccttg tgatcccggt acagtttgat gagttccttg ttcaaacgtt
      241 cgcgatcgtc cttaaatttc tcctggaccg cagcaacctt cggctggatg ccctgcatct
      301 tcttcatcga tgtataactt ttgtattgca gggggacaaa cagcagcttg atgatgacgg
      361 tcaagaggat gatcgtcagt ccataattat gtgtgtactc attgatataa cgcagcacgt
      421 agaagatggg cttggccacg gctctgaccg tatcccagct tccgaaaaga aaccatccaa
      481 aatcgatggt atcttctagc ctgatttgca aactctgaag ggtgtcaaac tccttgggac
      541 cggcgaatag ctggaggccg agaggtgccc cggacgaatc gacagccaaa cgaaccgcag
      601 tggaaacaag cttgtcccct tgtttcctgg gaaaagcgga cgcattcgtc tttggaataa
      661 gggcggcaat gaaatacttg tcttgtagcg cgacccattt tagggcgcct ttgcgctcag
      721 tctcggattc cggcatgtcg ttttctatct tatcgccgat gagggaagct gctccgacgg
      781 aaccgatgaa tccttctccc caatccacga ccccgaaatt cgttcccaat ccgactttca
      841 cccctccgag tccgctgggt ttcaacgcga tgtctacgac atagctatcg gcgtgaaatg
      901 tcagttgctt ttccaacagt acgccggtcg ccggattagc gtaatagaag gtcatatggc
      961 cagttgggtg gccctggtcc aaggtcgtaa agtccttttc aaccctgtag atcccttcgc
     1021 ttaattcttt tgaatgagcg gcatcatcag cggtgatcgt cagcggctcg gcaaacttgc
     1081 ctccttgtcg cacgagttgc acggcggact tcccatccga actgttttga tagcgtttga
     1141 gttcccagct aatcagcgcg gccccgcgag ttgtgaactt agcccgatag agatccgtct
     1201 ctacttcgac gaccttcatt gtcgaaggct ccccgagcgg ttcttgagtc cggcggtcct
     1261 cagcggaggg ttttaggggc actgtttcct tggtctcggg agcgcttgaa gttggagcgc
     1321 cggggcttgt actcgaaggg gaagggaatg tcgtatcgct cgtggaaggg tcagccggtt
     1381 ccgaaatggt cggttgagga agtaggccca gctctttgag caggaactcg tacccaaaga
     1441 taattgcaag ggagagcacc aagaacacga tgactcgctt ttccatgaag agactatgct
     1501 ttcttaaaag atgttatcgt cgctatttga ccgggtcttc tcccccggga tgaaaggggt
     1561 gacatttcat aagccggaga atggctaggc cgatcccctg taacgttccg tatttgatga
     1621 ttgcatccga ggcatattgc gaacaactgg gttcgaatcg gcatgtacga ccatacaaag
     1681 gggaaatcac caggcgatat cctcgaatca gccgcagaca cagccattgc atatcaatat
     1741 gtccttggtc gaagaaggtg caggtgctca agcgagcccc tccacgcttg gaccaactca
     1801 tcccgcgatt gcaatagtgc gtcccgcttt ggaaacacga gaagtcctcg gcccggaacc
     1861 aagtccggat gtaactgcct taccagttcg cgaaagatcc gtttggcccg attgcgtcgt
     1921 acagcatttc caaatcgctt tcccacgata atcccaacgc gagtcggacc atcatccatc
     1981 ttgcacacca agagattgaa gaggacggtc gtaattcggc ggccatgatg tttgacgatt
     2041 tcaatgtccc ggttactgcg caaaaaaatg acatgtcgcc cctgcgcgtt cggtgtcatt
     2101 ggaaaggggg tgtttcttcg cgccgcgttc catccgttaa tgatcccgga gaagaatcta
     2161 gctggtaggt gaacgcacgc ccaaccgcaa cgcgagagat cgagagcgac ccaaagaaca
     2221 cctgcttatc cacacgatgt ggctagacag tcaaccgagc tcgtcctttt gccctccggc
     2281 gggccagcac cttgcgtccg tttttagtgc tcatacgctt cctaaatcca tgctcacgct
     2341 tcttttttaa gttggacggt tgttgaaatg taaaagacat aatccccctt ccattcccac
     2401 gctttaaata gtcagttcaa atgaactggg gattataggg gaggcacttg ggcctgtcaa
     2461 tttgatgctg cctctgacat ggccatcaga gacttttaag aatgcccaac gtcagtcatt
     2521 catatctaca gcgttgaggc gcttttgctt tctgtcacca gaatgccaca aaaatcggtg
     2581 tcttgaggac acaaactcat cataaatatg tgaaatagat tgaatcttta tccgaccaac
     2641 gaccattatc gatggcaccg agcttgctgc atattttgtt cgtgctagta tgccggacgg
     2701 tctaagtctg ttaagctggc gtatctataa gatagtctta atgggttttg aagagtaatg
     2761 ccggctgaaa atgatggact gttacattaa agacgaagga agaatcggat tgaccaaagt
     2821 ctgtggatag aacctgtaag gaggattcct cgtatgctga gaaaagcatc ccttgtctct
     2881 ctttccttga ctctcgttgt gatggccgga tgtgcggagc ctcccactca gcagatccag
     2941 gaagcggaaa aggctctcaa agatgcacaa gagagcggcg ctgccactta tagcgctgat
     3001 gagtatgcca agctagaagg gactctttcc gcaatacgga aagaagtcgc cgatcaggat
     3061 ggaaagttca cactctttcg agactatagc aaggcacaac aactctctgc ttcggccaaa
     3121 gccgatagtg aacggatcaa agcggcctcg tctcaaaaaa aggaagaagc caaggcggcg
     3181 gctttgcaag ctcaacaagt cgctgaagag gcggtgaagg ccacgcaaga tctggtcgcc
     3241 aaggcgccgg tcggaaaaga tcgtgcggcg gtggaggcca tcaagaacga tgtggaaggg
     3301 ctgaagtcgt tactgaaaca ggttcaggtg tcgatcgaca aagaagatta tccggccgcg
     3361 caaacgcagg cgaaggccat tcacgacatg agccaaggag tgtcgacaga aatacagagt
     3421 gctctcgcga aggtgggaaa aggaaaactt gcaaggaaaa agtaatacac ggcatggcgc
     3481 ttgatcagac acataagacg cttgccgtga ttctctgcgt cctctgcttg accggttgcg
     3541 ttcaagctgt tccgccggat ctccttgtag ctcttgaggc cattgatcaa gatttgatcg
     3601 cattgcgtgc tccggaagcc gcacctgcgg aatacgggca gttcgttcac caatgggtct
     3661 ctctgaaagc gcgcgtccaa ttggatgatg acctgatcag atggccatgg gaatcgaacg
     3721 atcttgagaa tgaactccgg cggctataca tcatgggcga acgtactgtc tcccagctgc
     3781 acgagagaca agcctcacag catcgcgtgg cacaggccaa ggtggttcgc cttgaagaac
     3841 ggcttcatac gattacctcg agggtcgaat cgatcgatgg gcgtatcctc ctaggagaca
     3901 aagttgttca aacggatctc cttgtcaaac aggcccgttc cttcttcgaa caaaaagact
     3961 tccaacgagc catgcaagcg gcagagaaag cggatcatgc cctcaaggct caggcttcat
     4021 tactcagtca agaattggga cgatacgccg acgagaggcg gatcgcctat tggcagacct
     4081 tggccaagca gactattgct tggtcgcgaa tccaccaatc aacggccatc gtggtgagca
     4141 aggccgaccg agagttgact ctctacaaga gcgggcggaa ggtgctatca tatcccgtca
     4201 ggttggggtt caatggcatg ctggaaaaac agttccaggg ggacggagca acaccggaag
     4261 gtcggtatcg tgtgacggac aagcgcggcc aaggacagac gcaattctac cgagccctgg
     4321 ctctggatta tcccaatgcc gaggatcgtc ggcggttcga gcttgcgaaa agatcgggaa
     4381 ggattggccc ggccaaaggc attggcggcc aaatcgagat ccatggagcc gacaatgaac
     4441 tgttggccca gacccttggg tgcatcatgc tcgataatcc aaatatggct gttctctacg
     4501 agggggtctt cgtcgggaca ccggtcacga tcgtcggtgc cttgacgaga gaaaatgcgg
     4561 tggccctggc cttatcggag ctcgcacagc gcaaagacaa aatctgagca taaactgatg
     4621 cgccgaatcc atcttgcagc cgcagcgatc actttactcc tactactgat cgtgagcgct
     4681 ctactcgtcg tgagtgaccc gactcccagt cgcgtggcgt ccgaagattc ccaagtcgtt
     4741 caccagcccg ctgcccaaga caaagccggt cttcgaacct tgcaagccag gtataaggtg
     4801 ctctccaagc agctgtccca actcatgccg aaccagcctt acatcttggt cgatacggcc
     4861 aggaacagac tctatgtgaa gcaccaagag caagtcgtcc tcgatgcaat cgcttcgacc
     4921 ggtagtggaa caattctcga taagcccggc gaaggtcaca gccagtggat tttcgatacg
     4981 ccacgaggag aatttctcgt caagtcgaaa ctgaccaatc ccacgtggat caagcccgat
     5041 tgggcgttcg tcgaagaag
//
LOCUS       DATNDD010000162         5381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3881511, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000162 DATNDD010000000
VERSION     DATNDD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3881511"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VN647_03875"
     CDS             <1..261
                     /locus_tag="VN647_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96203.1"
                     /translation="QNAISQSFIASSSRLLLWSMFPLMVGTCLDFYLISTMILSSAWW
                     GAVLSLALFTAFGTLWFGFPYVANKKKGALELTSNKKALSSQ"
     gene            589..1038
                     /locus_tag="VN647_03880"
     CDS             589..1038
                     /locus_tag="VN647_03880"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWG96204.1"
                     /translation="MSVIQKSIDLNVPVRTAYNQWTQFEEFPRFMEGVEQVQQIDDKH
                     IHWKANIGGKQEEWDAEITEQVPDQRIAWRSQQGAKNEGIIIFSPITEGKSKINLRID
                     YEPQGVVEKTGNAIGVVAQRVEGDLKRFKEFIESRGQETGGWRGNVS"
     gene            complement(2228..2398)
                     /locus_tag="VN647_03885"
     CDS             complement(2228..2398)
                     /locus_tag="VN647_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96205.1"
                     /translation="MSPDEAMQELLTGNQRTIDNQLTSIQQDPTVLREHTVDKPEPRS
                     SHRENAGSAGQP"
     gene            complement(2715..3467)
                     /locus_tag="VN647_03890"
     CDS             complement(2715..3467)
                     /locus_tag="VN647_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWG96206.1"
                     /translation="MITSTFCLLPGIGRTTERRLWWEGITTWSHFLAASSIRGLSAGR
                     KALYNESVSEAQSQYVRDNARYFGVVLPQREQWRLYEWLRQRAVYLDIETDSYGQITL
                     VGCYGQGRFISFVRGESLVARNLVEELSQYDLLVTFCGTTFDLPMLLAHYPDLPLDQP
                     HIDLCFVGRQLGYRGGLKAIEAQLDIARAADLHGVNAHDAVLLWNRWRHSHDEDARAR
                     LLGYNEADCVNLERLADIFYCEMVRQLVAVPR"
     gene            3874..4182
                     /locus_tag="VN647_03895"
     CDS             3874..4182
                     /locus_tag="VN647_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96207.1"
                     /translation="MDTVLLTGVPTKAFTVIENGPLPVLPEVIIEFQQGELHPELRAR
                     FDFPADAFTGEALIASADTTSLNTPPQRFVISRFVSTHFFEPSYVLVMTDGTMAAPFQ
                     "
     gene            complement(4678..4965)
                     /locus_tag="VN647_03900"
     CDS             complement(4678..4965)
                     /locus_tag="VN647_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96208.1"
                     /translation="MAFAGGIGSSGGARSPTPTQPRKYLNQFPEDFQMCFKVWEEITI
                     PSYAKHARYGLKAGQLNLFFLNAKMSTDLVLIPHRDTRLDPHTGPFLFEFQ"
BASE COUNT         1264 a         1453 c         1400 g         1264 t
ORIGIN      
        1 caaaatgcaa tcagtcaatc atttatagcg agttcgtctc gactcctgct gtggagtatg
       61 ttccctctga tggtcggcac gtgtttggat ttctacctga tcagtacgat gatcctctcg
      121 agtgcctggt ggggagcggt attatcgctc gccctattca ctgcctttgg aactttgtgg
      181 ttcggatttc cttatgtcgc gaacaagaaa aaaggcgcac ttgaactaac ttccaacaaa
      241 aaagcgctgt cttctcaata agactaaaaa ggttgggcgt atcttgccgt cactggacga
      301 cagatgtacc cataggtgat ggcctgccga gccggtgatc cccctgtatg tgtagtcttc
      361 tccgtccgat ccctatgtga acgtggctgg cgaccgagat aaagcattcc gctgcccaaa
      421 ccctgcctga tcgttggcct cttacagcgt ttgaattcct tccccttcgt tctgcactat
      481 gctctttcct agtatgacac gtgagtaaag tgtcatacat tgaggtaggc aatctgctcg
      541 acctgtcttg catcaacgtc agctacaatc aataaggagg tacctacaat gtctgtcata
      601 cagaaatcca ttgacctcaa tgttccggtt cgtaccgcct acaatcaatg gactcaattt
      661 gaagaattcc ctcggttcat ggagggagtt gagcaagtcc agcagattga cgacaagcat
      721 atccactgga aggccaacat cggtggaaag caggaggaat gggacgcgga aatcaccgaa
      781 caggttcctg atcaacggat cgcatggcgg agtcagcaag gggcgaagaa cgaaggaatc
      841 atcatctttt ctcctatcac tgaggggaag tccaagatca acttgcgcat cgactatgag
      901 ccccaagggg tggtcgagaa aacggggaat gccattggcg tggtggctca acgagtggaa
      961 ggggacttaa agcgattcaa ggaattcatc gagtcgcgcg gacaagaaac cgggggatgg
     1021 cgagggaatg tcagctgagc aaaaaaagac gtcctcgccg ggcttcgtgg tgacacactg
     1081 caggcgggct gcgcaaaccc aaggaagttg taacggaata ttcccagcga atgaggagcc
     1141 gcagcccata tcggctgatg aaagaaacga gagcccaata atgaccgtgc ccgactatat
     1201 tctttgatgg caccttgtcg cttcgaggcc gtccatcttc ggcatgttac tatccatcac
     1261 gatcacatcg agtggagctc ccgaacgagg accaccactg tgagcccacc tcttttttgc
     1321 cgatcacttc caaatgcttg tagacagata caatgcttcg cagtccgttc cgcatgagag
     1381 gctgatcatc catgggaaga agccgaatcg tcgtatattt ttctggtgct ggggactgat
     1441 tttgagaatt aggcgatcag aagaaggagc cgtcatgtga tcgctgatgc cgtcttcaac
     1501 ttgcattgtg acgggcagga tacgtgtcgc catctccccc cttccccgct ctgcttgaag
     1561 atcgaaaccg ccgcctagag ctttcattcg ttcccggcta ctgaatagcc caaaatttga
     1621 ggatagcggg gaggagctct caaagagaga gaagcagcca gattaaatcc aatgccatcg
     1681 tatatgacga cccacagcac tccattctca tagatcaatc gaactgtgac ctgtcttacg
     1741 gtgccgtgtt tcgcgacatt gatcagcaac tccccggacc gacgggtcca acgtgttctt
     1801 gattcccatc gctggaatat accttgggac ggcccttggc tctcgctctg gggaaggtcg
     1861 gatctccagc acactccgcc cgcgcgccct cgtcagcacg tgctgactcc tcgggaggcg
     1921 ctgcggtaga attctgcccc atgcccctct tgcccctgat acgattcggg tcttccccct
     1981 ggacgtatga agactggcaa ggccaggtct atctgaaaca atacgccaag acgaagtttg
     2041 caaaggaatg tcttggggag tattgccagt atttctataa cggcgaaacg atgccacctt
     2101 ctatcgacca ccgacaccga accaacttca caagtacctc aatcagatcc cggaagactt
     2161 tgaaacctgc ttcaagaggg ggaagaaatc accattccga gctatgcaaa acacgctcgt
     2221 tatggagcta aggctggccg gccgaacccg cattttctcg atgcgaagat ctcggctctg
     2281 gtttgtcgac cgtatgctct cgtagaacag tcgggtcctg ctggatggag gtcagctggt
     2341 tgtcgatggt ccgttgattg ccggtgagca attcctgcat cgcctcgtcg gggctcatga
     2401 gagatgtcag cctaacttgg agttcttaga tgcgaagaaa gcgttggaat gcaatgtgac
     2461 cataatgcca gcgggtttat caggagtctt ccagagcgag aattaaacac cagtcgtcat
     2521 aagaaatatg catgagagaa gcatcatgtc caacctcagc gaacctcgag ttcggcagat
     2581 gtgtgagcga tgtgagtgta ggatgaggta gtttctcgtg atcgttctga tctggccccc
     2641 agtgatatcg gcgtgagcag tcgaagacca gagaagggct caagacactc agtcaggttg
     2701 gccctatcga gttgttaacg aggtaccgcg actagctgac gaaccatctc gcagtagaag
     2761 atatcggcca gccgttctaa attaacgcaa tccgcctcat tgtagccgag caaccgagct
     2821 ctcgcatcct catcatggct gtgccgccat cgattccaca gcaacaccgc atcatgagcg
     2881 tttacgccat gcaggtctgc cgcgcgagca atgtccagtt gagcttcaat ggccttcagc
     2941 ccccctcgat atcccagttg tcgaccaaca aaacacaggt ctatatgagg ttgatctaaa
     3001 ggcagatcgg gatagtgcgc gagcaacatc ggcagatcga acgtcgtccc gcaaaacgtc
     3061 accaagagat cgtattgaga cagttcttcc acaaggttcc gcgcaacgag ggactcccct
     3121 cgaacgaagg agatgaaccg gccctggccg tagcacccca caagggtaat ctgtccgtaa
     3181 gaatccgttt cgatatccag atacaccgcg cgctgacgca gccattcgta gagcctccac
     3241 tgctcccgtt gcggcaagac cacaccaaag tagcgcgcat tgtcccgaac atactgggat
     3301 tgggcttccg agacactctc attatacagg gccttccggc ctgcgctgag acctcgaatg
     3361 gacgatgcgg cgagaaaatg tgaccaggtg gtaattcctt cccaccacag ccgccgttcc
     3421 gtcgtgcgtc ctatccctgg aagcaagcag aaggtggagg tgatcatcag tcgtctccat
     3481 cgactctttc gtcgagtgca gcaggccggg gcatgaaaca ctgcatggtt tcgctcattc
     3541 gtagccgaag cttttgatat tacatcattc gagtgaaatt ctcgtgcgtt ctacggactc
     3601 atatagttgt gcaatggttg aatgcaggcg atccatcgca tgagaggcag gctatgaggt
     3661 cgggcttcac ctgcagccta gaagccgcta actcaacggc cctgccctac tgaacttgtg
     3721 gtctgcgaca attgcgtctt gaccttctcc acgggtccat ttgtgtggct agttcgccag
     3781 gatggaagac ctgacggcta ttagttctcg ggtgggggtt cgctgcagat cattggggga
     3841 gccagtgctc gggatggaag ttggtccctc cggatggaca cggtcttact gacaggagta
     3901 ccgaccaagg ccttcacggt tatagaaaat ggtccccttc ctgtcttgcc tgaagtcatc
     3961 atcgaatttc agcaggggga actccacccg gagcttcgcg cacgttttga ctttccagct
     4021 gatgctttta ctggggaagc gctcatcgcc tcggctgaca cgaccagtct caacacccca
     4081 ccccagcgtt ttgtgatcag ccgattcgtg agcacacact tctttgaacc cagttacgtc
     4141 ctggtcatga cggatggaac gatggcagct ccattccaat gagctaagtc tctgccaagc
     4201 caataggatc atgactcacc aggctgcagc atcttctcaa gccttgcatc gtgcgcggtg
     4261 cattcccctc tgtccgatta ttgatgatca cgtaggcttg atgcttctcc tccatggcct
     4321 tcttcaccag gtccattatg tatgtctcgc cgcatttcgg gcagcttacc gacgatcttg
     4381 ttgtacggtt ccgctcgttt cttcgccgct tcgtaggata tcttcaatgg tgtgagcaga
     4441 cgcagggacc gtgaaggggg ccgtgaatgc ctccatgcat tgatgttggt ctgccaacga
     4501 gggcatgtaa gaccagtgat tgtagacgtg ggcgacgcca tgccgctcta agacctggcg
     4561 atgtactgga ccaggcgatc ccgtattccg aatctcaccc gcatactgga actctttcgg
     4621 cagctgcccg aagaaggtat gcagccacga gcaaaattct tggctcgaca tcccgtgtca
     4681 ttggaactcg aacagaaacg ggccagtatg tggatcaagc ctcgtatctc gatgtgggat
     4741 caagacaaga tctgtggaca tcttcgcgtt cagaaagaac agattgagtt gtccagcttt
     4801 caaaccatag cgagcatgct tcgcatagct gggaatcgta atctcctccc agaccttaaa
     4861 acacatctga aagtcttcgg gaaactgatt gagatatttg cggggttggg tcggtgtcgg
     4921 tgatctcgcg cctccggatg atccgatccc accagcaaat gccataccac cctaggtgcc
     4981 catgctttcg gatgattccc tggctaccaa atccgattcc cgagccgatg cccgcgccat
     5041 tggcacccct cgaaccacct agggtcgaat ccagaactcc tcggccctgg gggcatctcc
     5101 gctttgtgct ggtctcgtca tatcgacgcg acattggtcc atcggtctgg cagattctcg
     5161 accgtgggtc caatttcggt tcatagcccc tggatgcatc tgggcttcct gggagttccg
     5221 tagtaggaaa atgtccgggc gaaagaccga aacgagggaa atcagaccca agatcacgag
     5281 cgcaagattt ttgagttagg aacgtcatgg cgttaagagt ctctcgattt ccttaccatc
     5341 ccacaggcga cttgatcagg attgaattca tgctgcgtct c
//
LOCUS       DATNDD010000163         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2419708, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000163 DATNDD010000000
VERSION     DATNDD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419708"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1125
                     /locus_tag="VN647_03905"
     CDS             292..1125
                     /locus_tag="VN647_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96209.1"
                     /translation="MMGSSVQITGHSLRPALCVCLAALLGACGQLKPVNPLSSPTQAT
                     PEQSAEREREGQPPKVVQARPNSSSPQQCLINFDDENALEHIFGQARTTLAFRTSRIG
                     FGPLQMCDPSKHSDCWAYRQRCARSDVNFDTIGHTHFHLNMETGVECYTLPDPGDGLG
                     RGFGKLVDFKCTDVDWTKTPRVLSSHDQNQWIKIWVGNVDNHAPMYFDMESISILPDT
                     SIQLWFRKRDGTWWFWPELKAGRTWNIHNQVRDVSEVRIRGTKSGRASSYVIGSLVIR
                     D"
     gene            complement(1156..2475)
                     /locus_tag="VN647_03910"
     CDS             complement(1156..2475)
                     /locus_tag="VN647_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96210.1"
                     /translation="MGELGQTMLIRWADSLGAKREELVPLAWAFVYFFCLLCGYSILR
                     PVRDEMAIEGGLKHLPWMMTVTFLTMLGATPLFGWLSARCSRYRLLLTVYTFFITNLL
                     VFYALMTSHLYPEWVARGFFVWLSVFNLFIVSVFWSFMDDLFTPEQGARLFGVIAAGG
                     SSGALLGPLFTTGLTFVFPIQVLMAASSLFLGACIGCVYRLERWGRTQTLHHRPQQGE
                     PLRGSFLAGVRLTFSSPYLLGICGYLAILTMTATVLYFEQTRLVSEYLDRPEARTRLF
                     STLDFTTSLLTWLTQVFMTRRLIGRFGLVAALLSLPAISLVGFLGIALWPSLAVYVVF
                     SVLRRVGEYALSKPAREVLFTVVSREEKYKAKNFIDTAISRGGDASTAWLVTAVKAVG
                     AAPTHIAWTLVPLMGVWAWLATVLARQEKHRSASTLSSTTENSRSTA"
BASE COUNT          652 a          732 c          700 g          492 t
ORIGIN      
        1 tggaagcagg gccagatccg ccgatacctt cctgcactcc ttcaataact tatcgatgta
       61 taacttgact ggaacttcaa tcgtcatatg gggtccattg attcattgca atcgatccag
      121 ctccctgaga caatggcaca catttagggc gtcggactat accacgtgtt tttttcatga
      181 gtaaataacc ggcaagcctc gcggttcaac ctgctacaat tagaccttaa aatggcgaat
      241 aaggcgatgt gccggctcgc attggcgcaa gggatcaagg gataaggaga aatgatgggc
      301 tcatcagttc agatcactgg acatagtctc aggccggctc tctgtgtgtg cttggcggca
      361 ctcctaggag cctgtggtca actgaaaccg gtgaatcctc tgagttcccc gacgcaagcc
      421 acacccgagc aatcggccga acgagaacgg gagggtcaac ctcccaaagt cgtgcaagcc
      481 cgccccaaca gcagcagccc acaacaatgc ctgatcaatt tcgacgatga gaacgcgctg
      541 gaacatatct ttggccaagc cagaacaacg ctggcattca ggacgagccg aatcggattc
      601 gggcccttgc agatgtgtga tccgtcgaaa catagcgact gctgggccta ccgccaacgt
      661 tgtgcacgaa gcgatgtcaa cttcgacact atcggccaca cccatttcca tttgaacatg
      721 gaaacagggg tcgagtgcta tacccttccg gatccgggag acgggctcgg tcgagggttc
      781 ggcaaactgg tggactttaa atgcaccgat gtcgattgga cgaagactcc ccgcgtcctc
      841 tcctcccacg atcagaatca gtggatcaaa atctgggtcg gcaatgtcga caatcatgcg
      901 cccatgtatt ttgatatgga atcgatttcg atattgcccg atacctcgat tcaactctgg
      961 ttccggaagc gcgatggaac ctggtggttc tggccggagt tgaaggccgg ccgcacctgg
     1021 aacatccata accaggtacg cgatgtgtcg gaggttcgta tccgaggaac caagtcggga
     1081 cgagccagtt cttacgtgat cggctcgctg gtcattcgag attaggcaat aaagtccgaa
     1141 gcaagcggta ccgactcatg ccgtactgcg agaattctct gtcgtcgaag ataacgtaga
     1201 cgccgaccgg tgcttttctt ggcgagccag taccgtggca agccaggccc agactcccat
     1261 caacggaacc aaggtccagg caatgtgagt gggtgcggca ccgacggcct tcaccgctgt
     1321 cacgagccag gccgtcgatg cgtcgccacc acgcgagata gccgtatcga tgaagttctt
     1381 ggccttatat ttttcttcac gactgaccac cgtgaacaga acctcccgtg ccggtttgga
     1441 cagggcgtat tcccccaccc tccgcaacac ggaaaagaca acatagaccg cgaggctcgg
     1501 ccacaaggca atccccaaga atcccaccag actgattgcc ggcaaggaca gtaacgccgc
     1561 cacaagaccg aaccggccga tgagtcttct ggtcatgaat acctgcgtga gccaagtcag
     1621 gaggcttgtc gtaaagtcca aggtggaaaa cagccgagtc cgggcctccg gtcggtcgag
     1681 atattccgag actaagcgcg tctgctcaaa gtaaaggact gtggcggtca tggtcagaat
     1741 cgccaggtag ccgcaaatgc caagcaagta gggcgacgag aaggttagtc ggacgcctgc
     1801 gagaaagctg ccgcggagcg gctcaccctg ctggggccga tgatggagag tctgtgtccg
     1861 cccccatctc tccaatcggt acacacatcc gatgcaggct cctaaaaaca ggctcgatgc
     1921 cgccatcagc acctgaatcg ggaagacaaa cgtgaggccc gtcgtgaaga gcgggccgag
     1981 caacgctcca ctgcttcccc cggcggcgat cacgccgaac agccgcgccc cttgctccgg
     2041 tgtaaacaga tcgtccatga aactccagaa gaccgacacg atgaacaggt tgaacaccga
     2101 cagccagaca aaaaacccgc gggccaccca ctccggatac agatgactcg tcatcaacgc
     2161 atagaaaacc aacagattcg tgatgaagaa tgtatagacg gtcagcagca accggtagcg
     2221 cgagcatcgt gccgagagcc atccgaacag cggcgtggca ccgagcatgg tgaggaatgt
     2281 cacggtcatc atccagggaa gatgtttcaa acctccttcg atggccattt cgtcgcgtac
     2341 cggacggaga atggagtagc cgcacaggag acagaaaaaa tagacgaagg cccaagccaa
     2401 tgggaccagt tcttcccgct tcgcgcctag ggagtcagcc catcgaatga gcattgtttg
     2461 acccagctcg cccatgcgtg tccagtgtag caggtgtgct ttcacctgca agcgacagat
     2521 ctgttagaat actcggaccg tacggaattc ttaggagaca gcacagatga tcgacc
//
LOCUS       DATNDD010000164         4461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3713423, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000164 DATNDD010000000
VERSION     DATNDD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3713423"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VN647_03915"
     CDS             complement(<1..272)
                     /locus_tag="VN647_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWG96211.1"
                     /translation="MTGLAASPELLSALHNKATQLRINSVRATSEAGSGHPSSCASAA
                     DIVATLFFSVMRYDPHNPKAPNSDRFILSKGHAAPLLYAAWAEAGL"
     gene            complement(445..1071)
                     /locus_tag="VN647_03920"
     CDS             complement(445..1071)
                     /locus_tag="VN647_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWG96212.1"
                     /translation="MLKNAISPGFVDRNLTTIAIAQIIVLGLIALPLAGCGADGPDGP
                     AISSLSTPSNAQSGDDAQPDSNVAAMRSPDGEDPTAASPEHEAAEHATAHAAEDFEPA
                     TLAEPVEGDEDPTISVTSTSTGATAKLTWDTSHDPTVSNYYVYYGKQSSGEPGSCSYE
                     ASQSVETPPAIITGLEPNTPYFFAISAFGESESPCSSEVLVVTPPAQS"
     gene            complement(1320..2168)
                     /locus_tag="VN647_03925"
     CDS             complement(1320..2168)
                     /locus_tag="VN647_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWG96213.1"
                     /translation="MDAAVLVLITFVAATVNGALGYGFSSITVPVALLFYTNRILNPA
                     LVLIELVINSYVLFINRKSIPNIWRRVAPILIGLVIGIGIGSYILFLVQPAWIKFVTY
                     FFLLPLILLQAAGIRKPIQAEKAIAVPFGIGIGTLYSVTTISGPPLALLFNNQGYAKQ
                     DFRAALGVIRVAESSLTAIAYGLIGFYSAGSMDIIPYIVPSVIVGIPLGTYLIRLMDP
                     ETFRRICMAFDGLVVGFGLSRVLIELDLATSITTYSILSIVVLVNGYLLFRFFRDRAD
                     PQRIAS"
     gene            complement(2175..2609)
                     /locus_tag="VN647_03930"
     CDS             complement(2175..2609)
                     /locus_tag="VN647_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWG96214.1"
                     /translation="MKLSKKSEYGLRALLELTLVHGKTTLQRHDIAARQHIPVEFLEQ
                     ILLTLKRAGIISSRRGVNGGYVLIKQPNEITLGQVIRILDGPLAPIGCVSKTAYQKCN
                     DCPYANKMRCPVQQVMGTVRDAIAGILDHYSLDDFAAGYPKG"
     gene            2904..4265
                     /gene="radA"
                     /locus_tag="VN647_03935"
     CDS             2904..4265
                     /gene="radA"
                     /locus_tag="VN647_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWG96215.1"
                     /translation="MKSKIVFSCQACGYQAPRWLGRCPDCGGWNTMKEERQAATGKGR
                     PAAIKTAQAEVTPIGEIEIVGEDRRLTRIGEFDRVLGGGVIPGAVILIGGDPGIGKTT
                     LLLQALPRLATKEAPVLYVSGEESPRQIKMRGQRLGIEHPHLLILAETSLEQILKAVQ
                     EIGPAAVVVDSIQTVYTEQITSAPGSISQVQEVAGQLMWFAKRAGVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKGHSYRILRAVKNRFGSTNEIGVFEMKDAGLEEVDNP
                     SELFLAERPERSTGSVVVSSLEGTRPILVELQALVSSTTYAMPKRMANGVEVNRVSLL
                     LAVMEKRLGVHLSGQDVYVNVVGGMHIDEPAIDLGIVAAVTSSLREVPVEPGTLLLGE
                     VGLGGEVRAVSQAELRIRESAKMGFKRCILPERNLAKLDPIDGMELIGIQEVREALDV
                     VLA"
BASE COUNT         1069 a         1041 c         1337 g         1014 t
ORIGIN      
        1 aagagacccg cttccgccca cgccgcatag agcagcggag ccgcatgtcc ttttgacagg
       61 ataaaacggt cgctgttggg tgcctttgga ttatggggat cataacgcat gacggaaaaa
      121 aacaatgttg cgacgatgtc agcggcggag gcgcagctcg acggatggcc gctcccggcc
      181 tcgctggtgg cccgcacact gttgatgcgg agttgggttg ccttattgtg caaggcactg
      241 agtagttcag gcgaagcagc taaaccggtc atgggagatc ctttcgggat agagtaagga
      301 ggtggagtat ttgattgtcc actctcggca gcagcctgaa cttgaagcta acaaatcgac
      361 tgggtagagg tcaatgaaga gatgagctcc cctggaaaga accaggagag ctctgatgcc
      421 gtgatgacca ggcgaacggt ttcctcagct ttgagccggg ggtgtgacca caaggacttc
      481 actcgaacag gggctttccg actcgccgaa cgcacttatt gcaaagaagt agggtgtatt
      541 gggctcgagt ccagtaatga ttgccggtgg agtctcgacc gattgacttg cctcgtagga
      601 acatgaacct ggctctccgg atgattgctt tccgtaatag acatagtaat tggatacagt
      661 ggggtcgtgg gaggtatccc aggtcaattt tgcggtagcg ccggttgagg tggaggtcac
      721 agagattgtc gggtcttcgt ctccctcaac cggttctgca agggttgcag gctcaaaatc
      781 ttcggccgca tgtgcggtcg cgtgttctgc agcttcgtgt tctggtgatg ctgctgtcgg
      841 atcttcccca tcaggagatc tcattgcagc aacattagag tccggctgcg catcgtcacc
      901 cgattgagcg ttggatggtg tggagagaga cgaaatagcc ggtccatctg gtccgtcggc
      961 cccacatcca gctaacggga gggcgatgag gcccaaaact atgatctggg cgatcgcaat
     1021 cgtagtgagg tttcgatcaa cgaagccagg cgaaatcgca tttttcagca taagaaactc
     1081 ctctcagatt tagtatgtat gaagcgataa cggctcaagt gtcaaagcaa gggttatgcc
     1141 cagtgggcaa tgtaaaaact catttattat tcgatatttt gtaaatatga aaaacggcca
     1201 agatgctagg tgtgaagggt tcaaccttca agtaccgcca acagccataa caggtagtga
     1261 tgaaacccgc gtcaggtttt gccgcactca cgactcttgt ggaataacca aaggagggat
     1321 caagaagcga ttcgttgggg atctgctcga tctctaaaaa atcgaaacag gaggtaacca
     1381 tttaccagaa ccacgatgga caagatactg taagtcgtta tggatgtggc aagatcgagt
     1441 tctatcagta cccgggacag gccgaatccg actactagcc catcaaatgc catacaaatg
     1501 cgtctgaacg tttcgggatc catcaatcgg ataaggtagg ttccgagtgg aataccaaca
     1561 atgacgctcg gaacgatgta ggggataata tccatactgc cggcgctgta gaagccgatg
     1621 agtccataag caatcgctgt gagggaggat tcagcgacac ggatgactcc caaggcagcc
     1681 cggaaatctt gtttggcgta tccctgattg ttgaagagga gggcaagagg ggggccggaa
     1741 atggttgtga ccgagtagag cgtcccgatt ccgattccaa acgggacggc aatcgccttt
     1801 tcagcttgaa tgggttttcg gatgccggca gcttgaagga ggatcaacgg taacaaaaag
     1861 aagtaggtga cgaatttgat ccatgctggc tgaaccaaaa agagaatgta gctgccgata
     1921 ccgattccga tgaccaaacc gatcagaatg ggggcaaccc gccgccagat attggggatg
     1981 ctcttccgat tgatgaacag cacgtagctg ttgatgacga gttcaatcag cacaagggcc
     2041 gggttcaaga tgcgattggt gtagaacagc aaggccacgg gaacggtaat agaggaaaag
     2101 ccgtacccta acgcgccgtt caccgtggcg gcgacaaacg taatgaggac aagaacggct
     2161 gcatccatga gtgcttagcc tttgggatag ccggcggcaa agtcgtcaag ggaatagtga
     2221 tcaaggatcc ccgcaattgc atcacggacc gtgcccatga cttgttgtac gggacatcgc
     2281 attttattcg catacggaca atcgttgcat ttctggtacg ctgtcttgct gacacagccg
     2341 atcggcgcca gtggaccatc gagtatgcgt atgacttgac cgagtgtgat ctcgttcggt
     2401 tgtttaataa ggacgtatcc tccgttcacc ccgcgtcggc tggaaatgat cccagctcgt
     2461 ttgagcgtga gcagaatctg ctccaagaat tcaacgggga tgtgctggcg agcggcaatg
     2521 tcgtggcgct gcagcgtcgt ctttccgtgg acgagcgtga gctcaagcaa cgctctgaga
     2581 ccatattcac ttttcttcga gagtttcata agagtagagt gagttagtca acaatcgtta
     2641 catagggtaa actttggtgg ttcgtcaagg tcaaagttcc taagtgagta caaccgggta
     2701 ggagtccggc gaccgcgatg ctcttggaac aaagattgtc ggggatgcgt cgtgcgccgt
     2761 aaaggcgtat gcatattgga ggacatctga ggcatctcaa atggttccct cacctcatac
     2821 acgtccactc ttcccggctt ttattccttt tgtggattca ttaagatagg cacctggtat
     2881 cgtcctcctc cacgaggcca tttttgaaat ccaagattgt cttttcttgc caagcctgtg
     2941 gatatcaggc gccacggtgg ctcgggcgct gtcctgactg cggcggttgg aatacgatga
     3001 aagaggagcg gcaagcggcc accggaaagg ggcgacctgc cgccataaag acagctcagg
     3061 ccgaagtcac acccatcggc gaaatcgaaa tagtggggga ggatcgtcgg ctcacccgta
     3121 ttggtgaatt cgatcgtgtc ttgggcggtg gggtgattcc cggcgcggtc atcctcatcg
     3181 gcggcgatcc gggaattggg aagacgacgc tgctcttgca ggccttgcca cggttggcga
     3241 cgaaggaggc gccggttctc tatgtctccg gcgaagagtc tcctcggcag atcaagatgc
     3301 gtgggcagcg gctaggtatc gagcatccgc atctgttgat tttggccgaa acttcccttg
     3361 aacagattct gaaagcagtc caggaaatag ggccggctgc cgtcgtggtt gattcgattc
     3421 agaccgtgta cacggagcag attacatccg cccccggcag catcagtcaa gttcaggaag
     3481 tcgccgggca gctgatgtgg tttgcgaaac gagccggtgt cccggtcttt atcatcggac
     3541 atgtgacgaa ggaaggggcg attgccggac cacggctgct ggaacatatc gtcgatacgg
     3601 ttttatactt tgaaggagat aaaggacaca gctaccgaat tctccgcgcc gtgaagaatc
     3661 gttttggatc gacgaatgaa attggggtat tcgaaatgaa agatgcggga ctcgaagagg
     3721 tggataaccc atctgaattg tttctggcgg aacgccctga acggagtacc ggatctgtgg
     3781 tggtatcgag tctcgaaggt acgagaccga tcctggttga actgcaagcg ttggtttcct
     3841 caaccaccta tgcaatgccc aaacgaatgg caaacggagt ggaggtcaat cgagtctcgc
     3901 tgctgctcgc cgtgatggag aaacgactcg gagtgcatct ttcaggacag gatgtgtatg
     3961 tgaatgtcgt tggaggcatg catatcgacg aaccggctat tgacttgggg attgtcgcag
     4021 ctgtcacatc gagtttacgg gaggtgcctg tcgagccggg gacgttacta ttgggagaag
     4081 taggtttggg gggtgaggta cgtgcggtta gtcaggctga attgcgtatt cgcgagtccg
     4141 ccaagatggg gttcaagcga tgcattctgc ctgaacgaaa tttggcgaag ctcgatccca
     4201 tcgacggtat ggagctgatt ggtatccagg aagtgcggga ggctttggat gtggtgttgg
     4261 cgtgaagaga ggcatggcga agcggaaggg tctcttactg cgcgcgcgcg cccaacgcgc
     4321 ggcttcggaa agccctcgtt ggatgcgcgc gcgcgcagta agagaccctt ccactccgcc
     4381 atgccatagg ggattgagga aaataggatg ctgagacgtg cctatcccct tacattttta
     4441 acggcgttcg ggctcctctt c
//
LOCUS       DATNDD010000165         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_42914, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000165 DATNDD010000000
VERSION     DATNDD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42914"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VN647_03940"
     CDS             complement(<1..1580)
                     /locus_tag="VN647_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HWG96216.1"
                     /translation="MSVFKNLTTVRKLMLAFGTVGAIMAGLGCFGINTVSTVNDMVAV
                     LYERELKGLSAIKDVNLAITGISRLTRAAIISTDQKEKEQFSKTIVQLKQQFQDKLSE
                     FQKLTITDEAKTISAELARIFPPYMELMEEAVRLTLRDDNVNAIKKVHDAISDAQQVM
                     SKASELAVMKEKQAERVYKESNVLYERSRNIMVGLTIVGVGLGLMLGWVISQMIGRSL
                     AHLGKVVSKLERGDLTARAAVESKDEVGQLAQSFNKMGEQLEHTVKNQEQVIASKVAE
                     VKGITDAIDKSQAAIEFNMDGTIITANQNFLNCLGYTLDEIKGRHQRMFAEPAHAAST
                     EYQAFWAKLNRGEFDAGVYRRVGKGGKEIWIQASYNPILDSNGKVYKVVNYATDIMAQ
                     KTAQVEMERLVAESQTVLSKLAANDLTQEMTEAYSGELKKIKDNINAVVQHLTETITT
                     VRAAVEAVKDGSEEITRGNEDLSHRTSEQASALEETSASMEEMTSTVKQNAENAKQAN
                     QLAIAARETADKGGAVTK"
     gene            complement(1758..>2892)
                     /locus_tag="VN647_03945"
     CDS             complement(1758..>2892)
                     /locus_tag="VN647_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWG96217.1"
                     /translation="AQGVLTAMASGDLTKQMTGAYQGEFEQMKMSLNTAITQLTQTVS
                     LVREAVEAVTAGSEEISKGSDDLAHRTSEQAGALEETSASMEEMTSTVKQNADNSKQA
                     NQLAIAARDIANKGGAVTTRAVDAMGEINKSSKKIADIITVIDEIAFQTNLLALNAAV
                     EAARAGEHGRGFAVVAAEVRNLAQRSATAAKEIKGLINESIQRVTDGTELVNQSGKTL
                     EEIVGSVKRVTDIIAEISAASQEQASGIDQVNKAITQMDEGTQQNAALVEETASASQS
                     MKEQANELLHKISVFKIHVSEAQKAGMPPVAKYRKHVAATIGKVYGKTEATLTSARKQ
                     EPKSHFQNEDIDRPQTGPDFRAAGVAVGNAKDRRSAGDDFEEF"
BASE COUNT          504 a          874 c          725 g          789 t
ORIGIN      
        1 ttttttgtga cggcccctcc tttgtccgcc gtctcccggg ctgcgatcgc caattggttg
       61 gcctgcttcg cgttttcggc gttctgtttc accgtgctcg tcatctcttc catcgacgca
      121 gaggtctctt ccaaggccga ggcctgttca ctcgtccggt gcgacaaatc ttcgttccct
      181 ctggtgattt cctcggagcc gtccttcacc gcttccaccg ccgcccgaac ggtcgtgatc
      241 gtctcggtca gatgttgcac gacggcattg atattgtcct tgatcttctt caactcgccg
      301 ctgtaggcct cggtcatctc ctgcgtcaga tcgttggccg ccaacttcga gagcacggtt
      361 tgggattccg ccaccaaccg ctccatctcc acctgcgccg ttttctgcgc catgatgtcg
      421 gtagcatagt tgacgacttt gtacaccttt ccattgctat ccaggatagg gttgtaggag
      481 gcctgaatcc aaatttcttt gcctcccttt ccaacccggc gatagacccc ggcatcgaac
      541 tcgcctcgat tgagcttggc ccagaaggct tgatattcgg tcgatgctgc atgggcgggt
      601 tctgcaaaca tccgctggtg acggcctttg atttcgtcca gcgtatagcc cagacagttg
      661 aggaagttct ggttggctgt aataatcgtg ccgtccatat tgaactcgat cgccgcctgt
      721 gatttatcga tggcatcggt gattcctttt acctccgcca ccttggacgc gattacttgc
      781 tcctggttct tgaccgtgtg ctcgagctgt tctcccattt tattgaacga ttgggcgagt
      841 tgtccgacct catctttgga ttccacggcc gctcgagcag taagatcgcc gcgctccagc
      901 ttcgagacga ccttcccgag gtgggccaac gaacgcccga tcatctgtga tatcacccac
      961 cccagcatca gccccaggcc caccccgaca atggtgagcc ccaccataat attgcgggag
     1021 cgttcataga ggacattgct ttccttgtag accctttcag cctgcttctc cttcatgacc
     1081 gccagttcgg atgctttact catcacttgt tgcgcatcgg agatggcatc atgcaccttc
     1141 ttaatcgcat tgacattgtc atcccgcaag gtcagcctga ccgcttcctc catcaactcc
     1201 atgtatggag ggaagattcg cgctaactcc gcagaaatgg ttttcgcttc atctgtgatg
     1261 gtcaacttct gaaattcgga caacttgtct tgaaactgct gcttgagttg aacgatcgtt
     1321 ttgctgaact gttccttttc cttctgatca gtggagatga tcgccgcgcg tgtcaaccgg
     1381 ctgatgccgg ttatggcaag attgacatcc ttgatcgccg acagcccctt gagttcccgt
     1441 tcatagagaa cggcaaccat atcgttcacc gtcgacaccg tgttgatccc gaagcacccg
     1501 aggcccgcca tgatggcccc taccgtgccg aacgccaaca tcagcttcct tactgtcgtc
     1561 aaattcttaa acacgctcat gtgtttttct tacctcctac tcatatgcca tttctaagta
     1621 tccgggggtt aggtcggtcg cgggcatgct gaatgtagtt ccgagatcag ctacgagtct
     1681 tgtcctcggg aatctgtcgt tcctgagaac aacaccccat ctgatatcta ttgttgtagc
     1741 ttctggagaa aagtgtccta gaactcttcg aagtcgtcgc cggcactgcg acgatctttg
     1801 gcgttgccga ccgcgactcc tgcagcacgg aaatcagggc cagtctgtgg ccggtcgatg
     1861 tcttcattct ggaaatggga tttgggctct tgcttgcgcg ccgaagtcag ggtagcctcc
     1921 gtcttaccat aaaccttacc aatcgtagcc gcgacgtgct ttctgtactt cgccactgga
     1981 ggcattcctg ctttctgtgc ttcggagaca tgaatcttga acactgagat cttgtggagc
     2041 aattcgttcg cttgttcctt catcgactgg ctggctgaag cggtttcctc aacgagggcg
     2101 gcattctgct gggtgccctc atccatctgc gtaatggcct tgttcacctg atcgatcccg
     2161 cttgcctgct cctgagaggc cgcactgatc tccgcaatga tatccgtcac ccgtttgacg
     2221 gatccgacga tctcctcaag cgtctttccg gactgattga ccaactcggt accgtccgtg
     2281 acgcgttgga tcgattcgtt gatcaagccc ttgatctctt tcgccgcggt tgcggaacgc
     2341 tgggctaaat tccgcacttc ggccgccact acggcaaacc cccggccatg ttccccggcc
     2401 cgcgccgctt ccacagcggc attcaacgcc aaaagattgg tctggaaggc gatctcgtcg
     2461 atgacggtga tgatatcggc gattttcttg ctgctcttgt tgatctcgcc catcgcgtcg
     2521 accgccctgg tggtgaccgc tccccccttg ttggcgatat cgcgggcggc gatggccaac
     2581 tgattcgctt gtttggagtt gtcggcgttc tgtttgacgg tggacgtcat ttcttccatt
     2641 gacgcggagg tttcttccaa ggcgccggcc tgctcgctcg tccggtgagc gaggtcatcg
     2701 ctgcctttgc tgatttcctc cgatccggcc gtaacggctt cgaccgcttc acgcacgagc
     2761 gacaccgttt gagtcaattg cgtgatggcc gtattgaggc tcatcttcat ctgctcgaac
     2821 tcgccttgat aggcgcccgt catctgcttg gtcaagtcac cggaggccat cgccgtcaag
     2881 acgccctgtg ct
//
LOCUS       DATNDD010000166         3232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_149022, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000166 DATNDD010000000
VERSION     DATNDD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149022"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VN647_03950"
     CDS             complement(<1..332)
                     /locus_tag="VN647_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWG96218.1"
                     /translation="MFRSKLKELADRSLMRRLSPLESGTGPTIRYAGRQVILLSSNDY
                     LGLATHPEVIRAAIHATEQYGTGSGASRLISGTLPPHMHLETSLATFKGTEAALLFGA
                     GYLANIGV"
     gene            493..1269
                     /locus_tag="VN647_03955"
     CDS             493..1269
                     /locus_tag="VN647_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96219.1"
                     /translation="MTYRIKVPPRTLPVDEAHLVSGLEHWVFGLANYRWSILVGVLLL
                     LMIGGGIWGVFWYDAQNAGKAQELEREATILFTRAANDPQKAANNLKEAIVLYKRVVD
                     EYPRTSTAPLAQFSLGNAYLQSNDLASAIEAYNRFISTYGSHVSLLGLVQQKLGYVYL
                     LKGDLDQAAKAYSAVLEIPGALNRDYALFELARLEENRSKPDEALKHYQNLMKTFPNS
                     SLTSEAAMRVKVIEAKRTPDPSPAATTPSASSSPSKPSKP"
     gene            complement(1557..2858)
                     /locus_tag="VN647_03960"
     CDS             complement(1557..2858)
                     /locus_tag="VN647_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWG96220.1"
                     /translation="MMTTCERQWGPQATIAAVLIATLLLPPFESLAQSTSAGEQSNPA
                     GEIKSPQVDDGIDSNLVPLEPELEPEQTVSPSEPPSTDGSVELPEQTPDKSTVDASGT
                     ASTDAQGPLYNIPVVIDQNVQSHIHFFNIAIRNRFEQWLLRFSRYRPLVENIFAEFDL
                     PSDLVNLSLVESGFNPYAYSRAKATGPWQFMKGTGQLYGLRIDHYVDERRDPIKSTVA
                     AARYLRDLYDLFGAWPLAMAAYNAGEGKVMRALQKAQAESFSDISKTKLIRLETKQYV
                     PRIMAATIIAKNLDQYGFTQDPVPLHQFEEVVVTRPLHFRAIANVTGIPYAELRLLNP
                     ELRRDATPPNDAAYHLKVPVGTSAKVVELINRVPTYKFPPLRAESRHVKIDTARRYRV
                     RMGDTLEKVSRRFHVPLKTLKTLNHLSSPAIKAGELLVITR"
BASE COUNT          735 a          840 c          911 g          746 t
ORIGIN      
        1 atgacgccga tatttgctaa ataaccggct ccgaacagaa gcgcggcctc tgtcccttta
       61 aacgtcgcga gggaggtctc taggtgcata tggggaggga gtgttccact aatcaggcga
      121 gaagctccgg acccggtccc atattgctct gttgcatgta tcgcggcacg gatgacttcc
      181 ggatgggtcg caagtcccag gtaatcattg gaggacagta agatgacctg gcgtcccgcg
      241 tatcggatgg tcggcccggt gcccgattcc aatggagata gtcggcgcat gagcgatcgg
      301 tcagcgagtt ctttgagctt gcttcgaaac atgctgattt gaaactttct ttagagcttc
      361 gtagagcctc gacaggccgt gaccggacaa cgagtgtgtt ccgattgcat tgacaagaag
      421 aattcatcct tagtataaga caaagattct ggggcagata gtctggtctc aaccgtgaag
      481 tgatgattgc tgatgacata ccgaattaaa gttccgcctc ggacgttgcc ggtggatgaa
      541 gctcacctcg tgagtgggct tgagcactgg gtgtttggac tggcgaacta ccgctggtcg
      601 attttggtgg gggtcctgct tctccttatg ataggaggag gaatctgggg cgtcttctgg
      661 tatgacgcac aaaatgccgg caaggctcag gaactggaac gagaagcgac catcctcttt
      721 acgcgtgcgg ccaatgatcc acagaaggca gccaacaacc tgaaggaagc gattgtcctg
      781 tataagaggg tggtcgacga gtatcctcga acttcaacgg ctccgctcgc acagttcagt
      841 cttgggaatg cttaccttca gtccaacgac cttgcgtcag cgatcgaggc ctataacaga
      901 tttatctcta cctatgggtc tcatgtgtcg ctcctcgggc tcgtacagca aaaactgggt
      961 tatgtctatt tgcttaaggg ggatctagat caagcggcca aggcctactc ggcggtcctc
     1021 gagatccccg gcgcgctgaa ccgggattat gccctctttg aacttgccag attggaagag
     1081 aatcgttcta aacctgatga agccttgaag cattatcaga atctgatgaa gaccttccca
     1141 aattcttccc tgacgagcga agccgccatg cgtgtgaagg tgatcgaagc caagagaact
     1201 cccgatccgt ctcccgctgc aacaactccc agtgcttctt ccagcccttc gaagccatcc
     1261 aagccgtaac cgagctaact tcccttggaa aacagaggcg cccgctcgca agggcccgca
     1321 ggctggtcaa aaaggccgtc cggcaaggcc gcagaaaggt ccgcggcgcg aagaataatg
     1381 agcgccaagt ttgcggacgg gcgcaagttg gtgagcgccc agtatcttaa gggcgaagcg
     1441 tagaggtttt cacccaccca cccatcgcct gttggaacag gcgttttcac agggggctgt
     1501 acgttgagcc tctaagcgcc gtgagaacgc caccggcgga ctttttcaac agcctatcaa
     1561 cgagtgatca cgaggagctc gcctgcctta atcgctgggc tggacagatg gttcagcgtt
     1621 ttgagggttt tgagaggaac gtgaaatcgt cgggaaacct tctcaagcgt gtcccccata
     1681 cgaacccgat atcggcgtgc agtatcaatt ttgacatgcc gactttcggc gcgcagggga
     1741 gggaacttat aggtagggac tcggttgatt agttcgacta ctttggcgct ggttcccacc
     1801 gggaccttca gatgatacgc tgcgtcgttc ggaggtgtcg catcccgccg cagctccgga
     1861 ttcagtaatc ggagctcggc gtatggaatc cctgtcacat tggcaattgc gcggaaatgc
     1921 aaggggcggg tcaccacgac ttcctcaaat tggtgaagcg ggacgggatc ttgagtgaac
     1981 ccgtattggt cgagattctt cgcaataatg gtcgcagcca taatccgggg aacgtattgc
     2041 tttgtctcca gccgaatgag cttcgttttt gagatatctg agaaggactc cgcctgagcc
     2101 ttctgaaggg ctcgcatgac ctttccttct cctgcattgt acgccgccat ggccaagggc
     2161 caagcgccga ataaatcgta gaggtcccgc agataccgcg ccgcggcgac agtggatttg
     2221 atcgggtcac gtcgttcgtc aacataatga tcgatccgca acccatagag ttgccccgtc
     2281 cctttcataa actgccatgg acccgtggct ttagctcgtg aataggcgta ggggttgaag
     2341 ccgctctcaa caagagagag attcacaagg tcgctcggaa ggtcgaattc ggcaaagata
     2401 ttttcgacca gcggacgata gcggctgaag cgcaggagcc attgctcaaa cctgtttcta
     2461 attgcgatgt tgaaaaaatg aatatggctt tgcacgttct gatcgatgac gacgggaatg
     2521 ttgtagagcg gaccttgagc atccgtactc gctgtgcctg aagcgtccac ggtggattta
     2581 tcaggtgtct gttcaggtag ctctacacta ccgtccgtcg atgggggctc agaaggagaa
     2641 accgtctgct caggctccag ctcaggttct aaggggacga ggtttgaatc aatgccatca
     2701 tctacttggg gcgattttat ttcaccggca ggattacttt gttcaccagc cgaagtgctc
     2761 tgggccagac tctcgaatgg cggcaacagc agagtggcga tgagcacggc cgcgattgtc
     2821 gcttggggcc cccactgcct ctcacacgtt gtcatcatgg ataacctcga tgatagcctc
     2881 gatcgcatca aagactatcg acccaccaga tacttgtcaa ggaactgcag tcggcgaaga
     2941 cgctggtacg ttcgtgtcat gtcggcgtgt catgtcggac agagagaatg gtgtgcggaa
     3001 aaacttagct tctccagcgt catcgcaaga gaaaaaatcc tgatgcagtc ttcgtttacg
     3061 ccggtcgatt tcttgacagc ctcccggtgc aaatgatagc gtaccgcctc tcctagtggt
     3121 accccaccag aatcgcattg taaggaggat cgatcaatgt tgaagcggta tctcttggct
     3181 cccggcccaa cgcccgtccc tccggaagtg ttgctggcga tggctcgtcc ga
//
LOCUS       DATNDD010000167         6727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3772676, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000167 DATNDD010000000
VERSION     DATNDD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3772676"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..945
                     /locus_tag="VN647_03965"
     CDS             97..945
                     /locus_tag="VN647_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWG96221.1"
                     /translation="MNVFRFLLDVLKTFLQQGCASLAASLAFFSLLSLFPLAFLVLYG
                     VSFFVSQDVIGEQFMLSFLREFLPSLGERLAEELHRISVLESVRWLVFLSFFWFGGLV
                     FYELDYTLNVVFESMPQRHPLISTAISIALVGSTGLLLLMAYVGTQTIAFLTTYAPKL
                     WGLDLVALAAHDFHLTYTLPFTLAFLAVSLLYRLVPRRRPRWRAAMAGALIFSVLWVS
                     AKLLFVSYSDYATVYTRLYGSLLEVVLLLLWVYYSAGLLLFGGIIAHKLQQLACSSPT
                     VSPDDA"
     gene            complement(987..2147)
                     /locus_tag="VN647_03970"
     CDS             complement(987..2147)
                     /locus_tag="VN647_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWG96222.1"
                     /translation="MRVPSWEIGRALGIPIRIHASWFLVFLLVTWTLSTGYLPESLPG
                     LTPERYWAMGGLAAVLLFLSVLLHELGHSYVALYYRIPIEKITLFIFGGVAHMRKEAP
                     TPLAEFLIALAGPAVSFVLGGLCFVFVELAEAIQRQHGLQGWIMLGALLGFVNMQLGL
                     FNMIPGFPLDGGRVLRAGLWAWGNDFHRATKQAAVVGLAFGLLLGLVGLVVMYGAVSG
                     GLPASMASNGGWILVIGMFLFAAALASRRQAAIRQSLATVPIRDLMVTTVTSIPSHCT
                     LDEAVNQYFQSYGYGGFPVLEDGRLVGLITVLEIQNVSPSLWLWRRVEQVMRPRSESL
                     VIEPDIPVIHAMERMAREGWDRLVVMQDEDIIGLVTHSAIVHFLQLRSSSNR"
     gene            2217..2936
                     /locus_tag="VN647_03975"
     CDS             2217..2936
                     /locus_tag="VN647_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWG96223.1"
                     /translation="MQRRVLLRLLFGMLSAATPGLFVPVVGAAQVIKSGPPTCPGVAL
                     TFDLCPVRKGSGYDQPLMDYLITHRIPATFFMSGKWMAKHEADVDRLLGLGFFEVGTH
                     GEVHAHLPMHDADEQRTEILGPVKLLRKHYAHEATLFRPPYGEYNDVTVAVVNMLGLR
                     FIQWNIESGDPDPTLSAEQILSGVAARAKPGSIIVFHANGKGKQTRQVIEPLTSNILP
                     RKGLQPMTVSELLNCTPRAHD"
     gene            2929..3408
                     /locus_tag="VN647_03980"
     CDS             2929..3408
                     /locus_tag="VN647_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWG96224.1"
                     /translation="MTDPVIRNMDPDDRESVVQLLGESDPWRKLGYSKDDWNRVFCPT
                     PHGRDCYVALLDGQVAGVAIVKQKFLLGDYLELLGVAVWARQKGIGGQLLRYIEELVF
                     KRTKNLFACVSDFNEPARVFYQKHGYQEIGPMPNFLIPGSAELLLRKTAGPARQKGQ"
     gene            3471..3878
                     /locus_tag="VN647_03985"
     CDS             3471..3878
                     /locus_tag="VN647_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWG96225.1"
                     /translation="MTDLRVKKFLHTRMRVSNLDETIRFYTTVLGLEVIERKTSPRGS
                     ELAFLKVPNSDELIELTGFPPSGPVKVQEDLVHLAFQVESLDETIASLNIHGVRITDG
                     PTKTSSGSRFIFIDAPDGYEIELIERPADVKIV"
     gene            4014..4883
                     /gene="dapF"
                     /locus_tag="VN647_03990"
     CDS             4014..4883
                     /gene="dapF"
                     /locus_tag="VN647_03990"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWG96226.1"
                     /translation="MKNGFFRGHGLGNDYLVVNPKEMTFKLTPKNIKAICDRNWGAGS
                     DGILAFVPSKRANFGLRIFNPDGSEAEKSGNGLRIFARYLHATGKTRKTRFTVETKGG
                     LAGIALHIDRHGDAAAATVEMGVATFKPDALPCSLNVPELVQQSIQAAGRTLTFTGVS
                     VGNPHCVVFKPVGESWSREDLLALGPALENHRLFPKRTNVQLAVPTGHKEIFILIWER
                     GAGETQASGSSSCAAASAAVRLGLVRSPVTVKMPGGVLHIDVAPDFSLTMKGPVAEVA
                     RGTLSQSFVRGLR"
     gene            4951..>6727
                     /gene="uvrC"
                     /locus_tag="VN647_03995"
     CDS             4951..>6727
                     /gene="uvrC"
                     /locus_tag="VN647_03995"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWG96227.1"
                     /translation="MTSDLHSKLAHLPGNPGVYLFKNAQGEIIYIGKAAVLADRVRSY
                     FQKGADHSPKTSLLVGHITDVETMVTRSELEALILESNLVKRHKPRFNIVLRDDKQYP
                     YVRLPVKDDFPRLSIVRRVQKDGALYYGPYTPANALRETLKVIKHVFPLATCTIDIDG
                     TADRACIEFEIKRCMAPCTGNQSMEEYHQIVKQVRQFLEGRDRELLDDLRTRMEAAAE
                     REEFEEAARLRDRLFKIDRMLEKQRITQTSATDQDVIGLARQGSAVDLQILFVRGGLL
                     IGRKDFFWPQSADAPDTELVRSAIEQFYNKDGQPPREVLAPTDLDDIALIEQWLSDKR
                     GESVRILAPERGAKHQLVLLAEENAAAAAADHLRDEELDRQAGEELKRLLRLNHAPRR
                     IEGFDISNTMGNQSVASMVVWEDGQMKKADYRRFKIQTVTGANDFASMKEVVTRRYGR
                     EENLAQPDLILIDGGLGQLAAALEGLKEAGRQGLPILGLAKARGDKEERIFLAGRKNP
                     IVLRPQSPSTHLLQRIRDEAHRFAITFHRKLRGKSLVGSRLDQVIGIGEIRRNQLLER
                     FGSLDKLASASDDALREIGLNAKIIV"
BASE COUNT         1523 a         1931 c         1858 g         1415 t
ORIGIN      
        1 gggatctacc ctaatccgct cgtgcagcta gccatgcagg cggtgctgac gttaagatga
       61 gaaagccgtg tgtcgcccat ccgcatgcgg agtctcatga atgtcttccg cttcctgctt
      121 gacgtcctga agaccttttt acagcagggt tgcgccagtc ttgccgcatc tttggctttt
      181 ttctcgctcc tctccctctt tcccctcgcg tttctggtcc tgtacggagt gagctttttc
      241 gtcagtcagg atgtcatcgg cgaacagttc atgctgagtt tcctcagaga atttttaccg
      301 tcgttgggcg agcggctggc ggaagagctc catcggatca gtgtgttgga gagcgtgcga
      361 tggctcgtat ttttgtcgtt tttctggttc ggcgggcttg tcttttatga gttggactac
      421 acgctcaacg tggtttttga gagcatgcct cagcgtcatc cgctgatttc aacggcaatc
      481 tccatcgcgc tggtcggatc gaccgggctt ctgctgctca tggcgtatgt cggcacgcaa
      541 accatcgcct ttttaaccac ctatgcgccc aagctctggg ggctcgatct tgtcgcccta
      601 gccgcgcatg attttcatct gacctacact ctgcctttta ccctcgcatt cctggcggtc
      661 agcctattgt accgccttgt tcctcgccgc cgcccgcgct ggcgagccgc aatggccgga
      721 gccctgatct tcagtgttct ctgggtctcg gccaaattgt tgttcgtcag ctatagcgac
      781 tatgccactg tctatactcg gctctatgga tcgttgctgg aagtcgtcct cttgctcctg
      841 tgggtctatt attccgccgg gctcttgctg ttcggcggca tcatcgccca caagctgcaa
      901 cagctcgcct gctcgtcgcc tacggtgtcg cctgatgatg cttagcaaag atgttgaagc
      961 ggatgacaga cggcgagtgc cgccgctcag cggttggaag agctacgtaa ctgcaaaaaa
     1021 tgcacgatgg ctgaatgggt cacgagcccg ataatgtcct cgtcctgcat gacgactaat
     1081 cgatcccatc cttcacgggc catgcgttcc atcgcatgga tcacaggaat gtccggctca
     1141 atcaccaacg attccgaacg tgggcgcatc acctgttcga ctcggcgcca gagccatagc
     1201 gagggcgaca cgttctgaat ctccaaaacg gtgatgagtc ccaccagtcg cccatcctcc
     1261 aacacaggaa agccgccata gccataggac tggaagtatt ggttcaccgc ctcatccagc
     1321 gtgcaatgcg atggaatgga ggtgaccgtc gtcaccatta aatcccgaat agggaccgtc
     1381 gccagtgact gacgtatggc ggcttgacgg cgactcgcca gagccgcggc gaagagaaac
     1441 atgccgatga cgagaatcca tccgccgttc gatgccatcg aagcaggcag cccgccgctc
     1501 acggctccgt acataaccac aaggccgacc agtccgagca gcaacccaaa cgccaggccg
     1561 actactgcgg cttgtttggt agcccgatga aaatcgtttc cccatgccca caatcccgcc
     1621 cgtaacaccc gtccgccgtc cagcggaaaa cccggaatca tattgaaaag cccgagttgc
     1681 atattgacga agcccagcaa ggccccgagc atgatccacc cctggaggcc atgctgccgt
     1741 tgaatcgcct cggccagttc cacaaagaca aaacagaggc cgccgagcac aaaactgacc
     1801 gccggcccgg ccaaggcgat aaggaattcc gcaagaggtg tcggagcttc tttccgcatg
     1861 tgagccacac cgccgaaaat aaacaacgtg atcttttcaa tcgggatgcg atagtacaat
     1921 gcgacgtagg aatggccgag ctcatgcagg agcacggaga ggaataacag caccgcggcg
     1981 aggccaccca ttgcccaata acgctcggga gtgagcccgg gcaaactttc cggcagatat
     2041 ccggtcgaga gggtccaggt gaccaggaga aagaccagaa accatgaggc atggatacgg
     2101 atcggaattc ccaacgctct gccgatttcc caagaaggta cgcgcatagg ccataccgta
     2161 acagccgact acgtaaacgg tcaattcttc ccgacggttc aggtatactg acccgtatgc
     2221 aacgacgtgt tctgctacgc ctgctgttcg gcatgttgtc agcagcaact cctgggttat
     2281 tcgttccagt ggtcggtgct gcccaagtca tcaagtccgg cccaccaacg tgcccggggg
     2341 tggccttgac gttcgatctc tgtcccgtgc ggaaggggtc cggctatgat cagccgttga
     2401 tggactatct gatcacgcat cgtatccccg ccacattctt catgtccggc aagtggatgg
     2461 caaaacatga agcggacgtg gaccgcctgt taggcctggg atttttcgaa gtaggtacgc
     2521 acggtgaggt gcatgcccac cttcccatgc acgatgccga cgaacaacgc acggagatcc
     2581 tcggacccgt caaactgttg cggaaacact atgcccacga ggcgacgttg tttcggccac
     2641 cgtatggaga atacaacgac gtgaccgtcg ccgtggtgaa catgctcggc ttgcgattca
     2701 tccaatggaa tatcgaatcg ggtgatccgg accccacgct ctccgccgag caaattctct
     2761 ccggagtcgc cgcgcgtgcc aagccgggga gcatcatcgt cttccacgcg aacggcaaag
     2821 gaaagcaaac ccggcaggtg attgaaccgc tcacatcgaa catcctgccc cggaaaggac
     2881 tccaacccat gaccgtcagc gagctgttga actgtactcc ccgagcccat gactgacccg
     2941 gttatcagga acatggatcc ggacgatcgt gagtccgtcg tccaactgct cggtgaatcc
     3001 gatccctgga gaaaactggg gtactcaaag gacgattgga accgtgtctt ttgcccgact
     3061 ccgcatggac gagactgtta cgtcgcgctg ctcgatgggc aggtggcagg cgtcgccatc
     3121 gtgaagcaga agtttctctt gggcgactat ctggagcttt tgggtgtggc ggtatgggct
     3181 cgccagaaag gaatcggcgg tcagctgttg cgatacatcg aggagctggt gtttaagagg
     3241 accaagaacc tctttgcctg tgtatcggat ttcaacgaac cggcgagggt cttctaccag
     3301 aaacacggct atcaggaaat cggccccatg cccaacttcc tgattcctgg tagtgccgaa
     3361 ctgctgttgc gtaagactgc cggaccagcc agacaaaaag gtcaatagtc attggttact
     3421 cgtcactggt atgatgaaag tatattcgct gttccaatga ccattgacca atgactgatt
     3481 tgcgcgtcaa aaagttcctc cacactcgca tgcgcgtcag caatttggat gagaccatcc
     3541 ggttttacac gaccgtcttg ggtctggagg tcattgagcg aaaaacttct ccccgaggat
     3601 ccgagctcgc ttttttaaaa gtgcctaaca gcgatgagtt gattgaactg accggttttc
     3661 cgccaagtgg gccggtgaaa gttcaggaag atctcgttca tttggccttt caagtcgaaa
     3721 gcctcgacga aacgatcgcc tccctcaata tacatggagt caggatcacc gacggtccca
     3781 cgaaaacctc ctcaggcagc cgattcatct ttatcgatgc gccggacggc tacgagatcg
     3841 aactgattga acggccggcc gacgtgaaga tcgtttaaga aagtcatcgg tcatgaagtc
     3901 attggtcatt gaaccagcca cgaactgttg actattgacc attgactaaa ggctcagtac
     3961 cctcgccctc cggcaacctt tatccattca caggacttca cagggagatt ggcatgaaga
     4021 atggtttctt tcgcggacat ggtctcggca acgactatct ggtagtgaat cccaaggaga
     4081 tgacctttaa actgactccc aaaaacatca aagctatttg cgaccgcaat tggggcgcag
     4141 gcagtgacgg gattttggcc ttcgtccctt ccaagagagc caatttcggg ctacgcattt
     4201 tcaacccgga cggcagcgag gcggaaaaat caggcaacgg cctcaggatc tttgcacgct
     4261 atctccacgc gaccggtaaa acgaggaaga cgcgcttcac agtcgagacg aagggcgggc
     4321 tggccggcat tgcgctacat atagaccggc atggtgatgc agccgccgca accgtcgaga
     4381 tgggagtcgc cacgttcaag ccggatgctc tcccctgttc actgaacgtg cccgagttgg
     4441 ttcagcagtc gatccaggca gccggacgga ccctgacatt taccggcgtg agcgtcggta
     4501 atccacattg tgtcgtcttc aagcccgtcg gagaatcctg gtcacgggaa gatctcttgg
     4561 cgcttggacc ggccctggaa aaccacagac tatttcctaa acggaccaac gtgcagctgg
     4621 ctgttccgac cggacataag gaaatcttca ttctgatttg ggagcgtggc gccggcgaga
     4681 cgcaggcctc cggctcttcg tcctgcgccg cagccagcgc agcggtacgc ttaggactcg
     4741 tgaggagccc cgtcaccgtg aagatgccgg ggggggtgct gcatatcgat gtggctccgg
     4801 acttcagcct caccatgaaa gggccggtgg ccgaagtcgc acgagggaca ttaagccagt
     4861 cgttcgtgcg agggctacga tagagagtcg tcgagctgta caatatggcc atgttgttct
     4921 tctctgccaa tgactaatga ccgtgaccaa atgaccagtg atttacactc caaactcgct
     4981 caccttcccg gcaatcccgg cgtctatctg ttcaagaacg cgcaaggaga aatcatttac
     5041 atcggaaaag ccgcggtact cgccgaccgc gtccgatcct atttccagaa gggtgcggac
     5101 catagcccca agaccagtct tctcgtcggc cacatcaccg acgtagaaac gatggtgact
     5161 cgatccgaac tcgaagccct gattcttgag agcaatctgg tcaaacgcca taaaccccgc
     5221 ttcaacatcg tgctgcggga cgacaagcaa tatccctatg tgcggctgcc cgtcaaagac
     5281 gattttcccc gactctcgat cgtgcgccgc gtgcaaaagg acggagctct ctactatggc
     5341 ccttatacac cggcgaacgc acttcgggag acgttgaaag tcatcaagca cgtctttccc
     5401 ctcgccacat gcacgatcga tatcgacggg accgccgacc gcgcctgcat cgaatttgag
     5461 atcaagcgat gcatggctcc ctgcaccggc aatcagtcga tggaagagta ccatcagatc
     5521 gtcaaacagg tccgccaatt tctggagggc cgggatcgag aattactgga cgatctccgt
     5581 acccgcatgg aagcggcggc ggagcgtgaa gaattcgagg aagcagcccg actgcgggac
     5641 cgcctcttca agatcgaccg gatgctggaa aagcagcgga tcacgcagac ctcggccaca
     5701 gatcaggacg tgatcggctt agccagacaa ggttcggcag tggacctcca gattctgttc
     5761 gtgcgcggcg gcttgctgat cggacggaaa gatttcttct ggcctcagtc ggcggatgca
     5821 ccggacactg aactcgtccg ctccgcgatc gagcagttct acaataaaga tggccagccg
     5881 ccgagagagg tcttggctcc gaccgacctg gacgatattg cgctgatcga acaatggctc
     5941 tctgataaac gcggggagtc tgtccgcatc ctggctcctg agcggggggc caagcatcaa
     6001 cttgttcttc tggccgaaga gaacgcggct gccgcggccg ccgatcactt gcgcgatgag
     6061 gaactggacc ggcaagccgg tgaggaactg aaacgattgc tgcgactgaa ccacgctcct
     6121 cgccggattg aagggttcga catctctaat acgatgggca accaatcagt cgcctcgatg
     6181 gtcgtgtggg aagacggaca gatgaagaag gcggactacc ggcgattcaa gatccaaaca
     6241 gttacagggg ccaatgactt tgcaagcatg aaagaagtgg tcacgcgtcg ctatggaaga
     6301 gaggagaacc tggctcagcc ggatttgatt ctcatcgacg gcgggttagg tcaactggct
     6361 gccgcgctgg aagggctcaa agaggccgga cgacagggtc tgccgatcct gggactggcg
     6421 aaggcccgcg gcgacaagga ggaacggatc ttcctggcgg ggagaaaaaa cccgatcgtg
     6481 cttagaccgc aatctccttc cacgcatctg ctccaacgca ttcgcgatga agcccatcgc
     6541 tttgcgatca cgttccatcg aaagctgcga ggcaaatcac ttgtcgggtc cagactggat
     6601 caggtcatcg gaatcggtga gattcggcgc aatcaacttc tagagcgttt cggcagtctt
     6661 gataaactgg cttcggccag cgacgacgcg ctacgggaaa tcggtctcaa cgcaaagatc
     6721 atcgtgg
//
LOCUS       DATNDD010000168         5330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_223841, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000168 DATNDD010000000
VERSION     DATNDD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223841"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VN647_04000"
     CDS             complement(<1..189)
                     /locus_tag="VN647_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96228.1"
                     /translation="MLVPRARTGVFAGSIAVLMVGLLILHLPTEVAKSFLSLFPRQIS
                     TPFEMENLITVAREDATAL"
     gene            complement(236..1120)
                     /gene="ttcA"
                     /locus_tag="VN647_04005"
     CDS             complement(236..1120)
                     /gene="ttcA"
                     /locus_tag="VN647_04005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HWG96229.1"
                     /translation="MIVPLAPTSTPKLDEPTEKIQTRLCRLVGQAIAAYRLIEDGDKV
                     MVCLSGGKDSYGLLDILLVLQRRAPIRFDLIAANLDQRQPGFPEHVLPQYLTRRGIPF
                     HVESRDTYSIVKRLIPEGQTTCSLCSRLRRGHLYRIATELGATKIALGHHRDDMIETL
                     FLNLFYTGKLKAIPPKLRSKDGRHVVIRPLAFVKEADLARYAGLRGFPIIPCDLCGSQ
                     EDLKRKKVKAFLQQWERESPGCSDSIAAALANVAPSLLMDSRLFDFKSVTAVANNRAE
                     GDVWLDEEPSDREDDRPS"
     gene            1194..1691
                     /locus_tag="VN647_04010"
     CDS             1194..1691
                     /locus_tag="VN647_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96230.1"
                     /translation="MAQSILFVCTGNVFRSMAAEYALRTQLGQPSSYRVESAGIEAKP
                     KTIHPVIFNRLIQKGANPSAHIPQRLTKKLIDRNDLIIAMGLGHREFIHQQFRLKVPL
                     FNEVSFGEEIPILDLHEALPDWERDIDEARAYVESVIDHIWESVPALIARLPHFQEDR
                     RPMKS"
     gene            1841..2902
                     /locus_tag="VN647_04015"
     CDS             1841..2902
                     /locus_tag="VN647_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HWG96231.1"
                     /translation="MDKTVSDVLKTPVVGQDEPQWVSWSDDALLDLPMCDLHVGIKGG
                     FLEQPIAELIQELERRGLLFHPYFWLSNEWFTPDGVPGIAVPFYLAHPRLAKLEQAQM
                     LEVEGGTTEWCLRILRHEAGHAIENAYQIRRRKTRRHIFGKSSQRYPMYYSPRPYSRS
                     FVRHLDLWYAQSHPDEDFAETFAVWLTPDSLWEERYRGWPVLKKLKYVEGLMKELRGV
                     SPSVTTREEVDPLPSLKKSLREHYEHKRRHYGIERQSQYDPDLKRLFSSLPSHAGKLS
                     AATFLNRFRREVRRKVASWTGEYQYTIDQVLEDMIQRCRELNLRVPVAEDQAKLDFTI
                     LLTVHTMNFLRSGRHRVAL"
     gene            2899..3921
                     /locus_tag="VN647_04020"
     CDS             2899..3921
                     /locus_tag="VN647_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWG96232.1"
                     /translation="MKRLRVLVLMHEDLVPPDEVNGYDLKTVEWRTEYDVISTLKKLG
                     HEVYPLGVKSDLGAIHSAIENRKPDMAFNLLEEFDGVAVYDQHVVSYLELLRLPYTGC
                     NPRGLMLARDKALTKKVLSFHRIPYPEFIDVPQGRTVKRPKWLSFPLIVKSVNEEASL
                     GISQASIVEDDDKLSERVAFIHNNVGSGALIERYVEGREFYVGVIGNGHLQVLPVWEL
                     IMDKLPEDAKRIATERVKWSRKYQQKYGITSREADNLPQGKTEEIQNLAKRVYRALGL
                     SGYARIDMRMAADGQLYVLEANPNPQIAEDEDFADSAKKADYNYTELLQEILNAGLRW
                     RPAKAA"
     gene            4073..>5330
                     /locus_tag="VN647_04025"
     CDS             4073..>5330
                     /locus_tag="VN647_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWG96233.1"
                     /translation="MTSHLERSTSAGTALTALGVVYGDIGTSPLYALRECFHISHSLS
                     VTLPTITGLLSLIIWALLLVVTVKYLLFVMRADNQGEGGILALMALGQRHREESAFPL
                     RIGPVIALGLLGASLVYGDGIITPAISVLSAVEGIEVETTAYRPYTLPIAIAVLLAFF
                     AIQSRGTARLGEWFGPIMLIWFVTLAALGIKSAVQTPEVFAAVSPHHAVQFLLDHPAQ
                     GFAILGSVFLVLTGAEALYADMGHFGKGPIRLGWYSVVLPSLVLQYLGQGALLIRQPE
                     AVTNPFYLLAPGWFLLPLVILATMATIIASQAMLSGAFSLTHQAIQLGYLPRMDIRYT
                     SASHIGQIYVPAMNVLMLIGTVGLVVLFGTSSNLAAAYGIAVAGTMVLSTLLISVVAR
                     RQWKWNLPAVVLVTSFFLIMDLSFFGA"
BASE COUNT         1177 a         1511 c         1447 g         1195 t
ORIGIN      
        1 gagggcggtg gcatcttccc tggccacagt gatcaggttt tccatctcga acggcgtcga
       61 tatttgccgg gggaacaagg acaggaaaga tttcgcaact tccgtgggga gatggaggat
      121 cagcaatcca accatcaata cggcgatact gccggcaaaa accccggttc tagctcgcgg
      181 cacaagcatg ggctgatctc actcagtcga attatcctcc ttcagagtcg ttcaatcaag
      241 aaggacgatc atcttctcgg tccgacggtt cttcatctaa ccagacatcc ccctcagccc
      301 gattattcgc taccgccgtc actgacttga aatcaaagag ccgagaatcc attaagagcg
      361 acggcgcgac attggccaac gcggccgcaa tgctgtcgga acaaccaggc gactcccgtt
      421 cccactgctg aagaaacgcc ttcaccttct ttcgtttcaa atcctcttgt gagccgcaga
      481 ggtcgcaggg aatgatcgga aatcctcgca atcccgcata tcgtgcaaga tcggcctctt
      541 taacgaacgc cagcggtcgg atgacgacat gccgaccgtc ctttgaacga agcttgggag
      601 ggatcgcctt cagcttgccc gtatagaaga gattcaagaa taaggtttca atcatgtcgt
      661 cgcggtggtg accgagcgcg atcttggtgg ctccaagctc cgtcgcaatg cgatagaggt
      721 gtcctcgtcg taaccgtgag cacaacgaac aggtcgtctg cccctctggg atgagtcgct
      781 ttacgattga ataggtatcg cgggattcaa cgtggaacgg aataccgcga cgagtcagat
      841 actgaggaag cacatgttcc ggaaaacctg gttgccgttg gtccagatta gccgcgatca
      901 gatcaaaacg aatcggggct cggcgttgca atacgagcaa gatatccagt aaaccataac
      961 tgtctttacc gcccgacagg cacaccatca ccttgtcgcc gtcctcaatg agccggtagg
     1021 cggcgatggc ctggcccacc agccggcaga ggcgtgtttg tatcttctcc gttggttcgt
     1081 ctaatttcgg cgttgatgtt ggagcaagcg gaacgatcat ggcaagattg tagcggctcc
     1141 cttatagtcc tgtcgacagg atcgcgctta agataaaggt acccgaaccg cctatggcgc
     1201 agtcaattct ctttgtatgt accggaaacg tcttccgcag tatggcggct gaatatgcgt
     1261 tacggacaca gcttggtcag ccatcttcct acagagtcga atcggcaggg atcgaagcca
     1321 agcccaaaac gatccatccg gtcatcttca accggttaat ccaaaaaggg gccaaccctt
     1381 ccgcacatat cccacaaagg ttgaccaaga aactgatcga ccggaacgac ctcatcattg
     1441 cgatggggct tggccaccgc gagttcatcc atcagcaatt taggttgaaa gttcccctct
     1501 tcaacgaggt gtcgttcggc gaggaaatcc cgatcctcga cctgcacgag gccctccctg
     1561 attgggagcg tgatatcgac gaagcgcggg cgtatgtgga atcggtcatc gaccacattt
     1621 gggaatccgt accggcgctg atagcacgac tcccgcattt tcaggaagat cgacggccga
     1681 tgaaatcgta gcgtcgattc aactcggaac cgtttgcaca attttctgct cgcctacctc
     1741 tcgaccattc ctgagaagcc tccccatcgg acgggataaa tattgcgtgc tatattttcc
     1801 atgaggggca caatgtgcgc atttcagagg tttgcctttc atggacaaaa cagtttccga
     1861 tgtactgaag acaccggtcg tcggacagga cgagccccaa tgggtatcgt ggtcggacga
     1921 cgccctgttg gacctcccga tgtgcgacct gcacgtggga atcaagggcg gatttcttga
     1981 gcaacccatc gccgaattga tccaagaact ggagcgccgc ggattgctgt ttcatccata
     2041 cttctggctc tccaatgagt ggtttacccc cgatggagtt cccggcatcg ccgtcccttt
     2101 ttacttggcc catcctcgcc tggcaaaact ggaacaagcc caaatgttgg aagtggaagg
     2161 cgggaccacg gaatggtgct tgcgcattct tcgtcacgaa gcgggtcacg cgatcgaaaa
     2221 tgcctaccaa atccgccggc gaaaaactcg ccggcacatt ttcggcaagt cttctcaacg
     2281 gtaccccatg tactactccc cccgccccta tagtcgaagt ttcgtccgcc acttggactt
     2341 gtggtatgcc cagagccatc cggatgaaga cttcgccgag accttcgcgg tctggctgac
     2401 accggactcc ctgtgggagg aacggtaccg gggatggccc gtgctcaaga agctcaagta
     2461 tgtggagggg ctcatgaagg aactccgagg ggtttctccg tccgtgacga cccgcgaaga
     2521 agtcgatccg ctccctagtt tgaaaaaatc ccttcgcgaa cattatgaac acaagcggag
     2581 acattatgga atcgaacgac agtcgcagta cgacccggac ttaaaacggt tgttttccag
     2641 tttacctagc catgcaggca agctgagcgc cgcaaccttt ctgaaccgct tccgtcgaga
     2701 agtgcgacga aaagtggcga gttggaccgg cgagtaccaa tatacgatcg accaagtgct
     2761 ggaggatatg attcagcggt gccgtgaact aaaccttcgc gttcccgtcg cggaagacca
     2821 ggccaagctt gatttcacca tccttctgac cgttcacacc atgaactttt tgcgaagtgg
     2881 acggcatcgg gtggccctat gaaacggctg cgggtgcttg tcctcatgca tgaagacctt
     2941 gtacctcccg atgaggtgaa tggctatgac ttgaaaaccg tggagtggcg gacggaatac
     3001 gacgtcatct ctacactgaa gaagctgggg cacgaggtgt atccgctcgg cgtcaagagc
     3061 gatctcggcg cgattcactc cgccatagag aatcggaagc cggacatggc ctttaacctc
     3121 ttggaagagt tcgatggcgt ggcagtttat gatcaacatg tggtgtccta cctcgaactc
     3181 ttgcgcttgc cctacaccgg ctgtaatccg cgcggactga tgttggcgcg cgacaaagca
     3241 ttaacgaaga aagtactgtc ctttcaccga atcccgtacc ctgaattcat cgacgtacct
     3301 caaggacgca cggtaaagcg gcccaaatgg ctatcttttc cgttgatcgt caaatcggtc
     3361 aacgaagaag cctcgttggg catctcccaa gcctcgatcg tcgaggacga cgataaactg
     3421 agcgagcggg tagcatttat ccacaataac gtggggagcg gggcattgat cgaacggtac
     3481 gtcgagggtc gcgagttcta cgtaggggtc atcggaaatg gacatttgca agtgctgcct
     3541 gtgtgggaac tcatcatgga caagctgccg gaagacgcca aacgcatcgc aaccgagcgg
     3601 gtgaaatgga gtcggaagta tcagcagaaa tacggcatca cctcacgcga agccgacaac
     3661 ttgccgcaag gcaagaccga agaaattcag aatctggcca aacgggtcta tcgcgcgctc
     3721 ggactcagcg gctatgcgcg aatcgatatg cggatggctg ccgatggaca gctctacgtc
     3781 ctggaggcca atcccaaccc ccagatcgcg gaagacgaag actttgccga ttcagctaag
     3841 aaggccgact ataattacac agagttactc caagaaatcc tgaacgccgg ccttcgctgg
     3901 cggcccgcca aagcagcctg atcatagaga cggaaccgca aacaccctca gtcttcgccc
     3961 aaccgccttg ttctgacttc ttgaaccggc taggatcgat gccatatgcc tggtatctac
     4021 ggtttgactc ttactcatct agacctacgc ctctggccgt tccgtcgcac cgatgactag
     4081 ccacctcgag cgctcgacgt ccgcgggaac ggcgctgact gcgcttggcg tcgtgtacgg
     4141 cgatatcgga accagtcccc tctatgcgct gcgcgaatgc tttcacatct ctcacagtct
     4201 gtccgtcacc cttccaacga tcaccggcct actctcgctc atcatctggg ccctactgct
     4261 ggtcgtcacg gtgaagtact tactgttcgt catgcgagca gacaatcaag gcgagggggg
     4321 aattttggcg ttgatggcct tggggcaacg ccatcgggaa gagtccgcgt tccccttacg
     4381 gatcggcccc gtgatcgcgc tcggcttgct cggagcttcg ttggtctatg gggacgggat
     4441 cattacgccg gcgatttcgg tcttgagcgc cgtggaaggg attgaggttg agacaacggc
     4501 ctatcgaccc tatacgttgc cgatcgccat cgcagtcttg ctggcgttct ttgcgatcca
     4561 gtcgcgcgga accgcacgac tcggcgaatg gttcggtcct atcatgctga tttggttcgt
     4621 cacgcttgcc gccttgggga tcaagagtgc cgtacagact cctgaggtgt ttgccgctgt
     4681 cagccctcac catgccgtcc agttcttgtt ggaccatccc gcgcaaggct ttgcgatatt
     4741 ggggagcgtg tttctcgtcc tcacgggagc ggaagccttg tatgccgaca tggggcactt
     4801 cgggaaaggg ccgattcgcc tcggctggta cagcgtcgtg ttgccgtcgc ttgtgctgca
     4861 atacttggga cagggcgcct tactgatccg acagccggag gcggtgacga atccgtttta
     4921 cttgctggca cccggatggt ttctcctgcc gttggtcata cttgcgacaa tggccaccat
     4981 catcgcctct caagcgatgc tgagcggtgc cttttccctg acccatcagg cgattcaatt
     5041 gggctatctg ccccgcatgg acattcgata cacgtctgct tctcatatcg gacagatcta
     5101 tgtgccggcc atgaatgtgc tcatgctgat cggtactgtc gggctggtcg tgctctttgg
     5161 cacctccagc aatctggccg ctgcctatgg gattgccgtg gccggcacca tggtcctttc
     5221 cacactcttg atttccgtcg ttgcccggcg ccaatggaaa tggaacctgc cggccgtggt
     5281 cctcgtcact agttttttcc tgatcatgga cctctcgttt tttggggcca
//
LOCUS       DATNDD010000169         2968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_894842, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000169 DATNDD010000000
VERSION     DATNDD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894842"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VN647_04030"
     CDS             <1..924
                     /locus_tag="VN647_04030"
                     /inference="COORDINATES: protein motif:HMM:NF045283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HWG96234.1"
                     /translation="LRAESPAADMKALAKSIVSGEQNDIFQAMKNTDAMLQKLDPETM
                     ESVTPLLVEPWMITMRGTLERAKAEAAKRWEGEVYPACQRNVEGRYPFRQVGGDAPIA
                     DLSDLVHPDNGLIWKFYQAELKPFIVEGTDRWEPRQWAGVSMGLSDEFLGSLVHARLL
                     SDSLFPKGSANPGVTFELYPYPPLGGVKSVTEIRLEVGGQPFRYRMEPQEWHEIKWPG
                     QTPAAGALLQIQVGGVWITKEFGDWWGLFRLIQAGQLKPMAGDTTYRLQWDFPTADGQ
                     SAKVQYDLRAAGHKNPFRPGLFEQFRCVEHL"
     gene            934..1905
                     /gene="tagF"
                     /locus_tag="VN647_04035"
     CDS             934..1905
                     /gene="tagF"
                     /locus_tag="VN647_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HWG96235.1"
                     /translation="MTEAVCGYYGKLPVSPEFLRFHAAGPEVRELDEWLERGIQYAKA
                     RAGAEWPALVAQADIWNFLFVPETNGRVVCGLVFASRDRAGRSFPFLTFLLLECRPVP
                     PAPWLIPLKCRVFLEKARRLIQQLRADLDWNTFRASAESLSPEVDSDAVIEAGFQEYL
                     AHTTIEEFRSRLGGASDHPQQEGRGPTTAFYRPSGSEPFGKQAGSGRYLPLLREQPVE
                     TYDVPFWLEFHARWSGVKTGPVSSSITFWNRAPSKVEPCVLLSIGRPSSNLARFIVSP
                     NETDEAWDAGVVCIENSPEGISSSSVSPMRAMEDRNISLKQYLDLLN"
     gene            2061..2852
                     /locus_tag="VN647_04040"
     CDS             2061..2852
                     /locus_tag="VN647_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HWG96236.1"
                     /translation="MKAKELLDANRLSAAILELNQEVKQHPTDLRIRTFLFELLCFDG
                     AHERAERQLDVIGLQNESAGIGVDVYRQLLRADKARRRWFTEGLKPTFLIDPPSWAHH
                     QLEAGHRLRGQQAEEATTLLEEAAHSRPKLTGLVNGMRCTEFRDIDDRLGAVLEVFFR
                     ETYVWLPTEQVRKITIPPPRQLRDLLWLPATIEIEEGAAGQVYLPVLYVGSQSENDDQ
                     LRLGRMTDWRELGNGLVGGVGQRMFLVDDREFSILEIREMEFQLE"
BASE COUNT          646 a          769 c          920 g          633 t
ORIGIN      
        1 ttgcgggcag aaagccctgc cgcagacatg aaggcgttgg caaagagcat cgtatcggga
       61 gagcagaacg acattttcca ggccatgaag aatacggatg cgatgctgca aaagctcgat
      121 cctgaaacga tggagagcgt cacacccttg ctggttgaac cctggatgat caccatgcga
      181 gggaccttgg agcgggcgaa ggcggaggct gccaaacgct gggagggtga agtgtatccg
      241 gcctgccagc gaaacgtgga gggacgctat ccgtttcgcc aagtgggagg ggatgcaccc
      301 atagcagact tatctgattt ggttcatcca gacaacgggt tgatatggaa attctatcag
      361 gcggagctga agcccttcat cgtggaagga acggatcgct gggaaccgcg acaatgggcc
      421 ggcgtgagta tggggttatc cgatgagttt ctcggttccc ttgtgcatgc caggctgctc
      481 tcggacagcc tgttcccgaa aggcagcgcg aatcccggcg tcacgttcga actctatccc
      541 tatcccccgc tcggtggagt caagtctgtc acagaaattc gtcttgaagt gggtggacag
      601 ccattccgtt atcggatgga accgcaggaa tggcatgaaa tcaaatggcc tgggcaaacg
      661 ccggcagccg gggccctgct gcaaatccag gtcggtgggg tctggatcac caaggagttt
      721 ggggattggt gggggctctt tcgcttgatt caggccggac agctcaaacc gatggccgga
      781 gatacgacat atcgccttca atgggatttc ccgacagcag acgggcagtc ggccaaggtc
      841 caatacgatt tgcgcgccgc cggccacaag aatcctttcc ggcccggctt gttcgaacag
      901 ttccgttgtg tcgagcacct ctgagggaaa gcgatgaccg aggcggtatg cggatattac
      961 ggaaagcttc ctgtttcgcc tgagttcctt cgcttccatg cggcagggcc ggaagtgcgc
     1021 gaactcgacg agtggctcga gcgcggtatt cagtatgcca aggctcgggc aggagcggag
     1081 tggccggctc tcgtggccca agcagatatt tggaatttcc tgtttgtgcc ggagacgaat
     1141 ggccgcgtcg tatgtgggct cgtctttgcc agtcgagacc gagcagggag atcgtttccg
     1201 ttcctgacgt ttcttctcct tgagtgtcga ccggttccgc cggctccatg gctgatccca
     1261 ctgaagtgcc gcgtcttcct ggaaaaggcc agacggttga ttcagcagct tcgcgccgac
     1321 ctggactgga acacctttcg cgcaagcgcg gagtcgttgt ctcctgaggt ggattccgat
     1381 gccgtgattg aggccggatt tcaagagtac ttggctcaca ccaccatcga agaattcagg
     1441 agccgtctcg gtggcgcatc ggaccatcca cagcaggaag gtcgtggacc gacaaccgcc
     1501 ttttatcggc catcggggag cgaaccattc ggcaagcagg ctggaagcgg gcgatatctc
     1561 cctctactga gggagcaacc ggtcgaaacc tacgatgttc ctttttggct tgaatttcat
     1621 gctcggtggt ctggcgtaaa gacggggccg gtgtcgtcat cgatcacctt ctggaaccgt
     1681 gctccgtcga aggtcgagcc gtgcgtcctg ctgtccatcg ggcgtccgtc gtcgaacctg
     1741 gcgaggttca tcgtctctcc caacgaaacg gatgaagcat gggatgcggg ggtggtttgt
     1801 atagaaaatt ctccagaggg catatcgagt tcatccgtca gtcctatgcg agcgatggag
     1861 gaccgcaaca tctcgctgaa gcagtatctc gacttgctga actgagtagg ttgggtcgca
     1921 agaaaagaag aggcggaacc ggacgatccg ccgttgatat gcgtaggaat catatacgta
     1981 ggaacacgtt gaggacgttg tgtttcattc tcgagttgcg taggatgccg tagtattacg
     2041 gcttgcgagt ggagggtctc gtgaaggcta aggaacttct cgatgcgaac cggctttcag
     2101 ccgcaatcct tgagctgaat caagaagtca agcagcatcc caccgacctc cgcattcgca
     2161 catttctttt cgagttgctg tgtttcgacg gcgcgcatga gagggcggaa cgccagctcg
     2221 atgtgatcgg acttcaaaat gaaagtgctg gcatcggggt cgacgtatat cgacagctac
     2281 tgagagccga caaggcccgc cggcgttggt ttaccgaagg gcttaagcct acattcctca
     2341 tagatcctcc ctcttgggcc catcatcagc tggaggccgg tcaccgatta cgcgggcagc
     2401 aggcggaaga agcgacgaca ctccttgaag aagcagcgca cagccgtccc aaactgacag
     2461 gactggtgaa cgggatgcgc tgtaccgagt ttcgcgacat cgacgatcgg cttggcgcgg
     2521 tcttggaggt cttcttccgg gagacttacg tctggttgcc gaccgagcaa gtgaggaaga
     2581 ttaccattcc gccacccaga caattgcgtg atctcctatg gcttccggcg accatcgaga
     2641 tcgaagaggg agcagccggg caagtgtacc ttcccgtact ctatgtcggt tcccagagcg
     2701 agaacgatga ccaacttcga ctcggaagaa tgaccgattg gcgagaattg ggcaatgggc
     2761 ttgtgggtgg ggtaggccaa aggatgtttc tggtcgatga ccgtgaattc agcatcttgg
     2821 aaatccgaga gatggaattt cagcttgaat aagcaggccg cttcctgagc acaaggacgg
     2881 atatggcgaa agcaccgact atcgacattg atgcgatcct cgcgccgatt ccaggcggga
     2941 accctgcggg aaaggacgtt cgcgatgg
//
LOCUS       DATNDD010000170         3542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2353448, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000170 DATNDD010000000
VERSION     DATNDD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353448"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VN647_04045"
     CDS             <1..590
                     /locus_tag="VN647_04045"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HWG96237.1"
                     /translation="RSMIMKSLVLGIGSLFLLAFVYLGISSGAKDAGGIPAEKVADML
                     HSVIESNRTFYTQHVVERMQAKGAAVASENWRSEKTLPLPAQFLQESARLSVNSKAKV
                     GYKLISLWPINKQNGAKSEAEHRGLMATQQNPDRPYTEVVMNGADAYLMAVYADKAVS
                     QACIGCHNAHQDSPRHDFKQNDVMGAIMITIPLNL"
     gene            complement(775..849)
                     /locus_tag="VN647_04050"
     tRNA            complement(775..849)
                     /locus_tag="VN647_04050"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(815..817),aa:Cys,seq:gca)
     gene            complement(999..2345)
                     /locus_tag="VN647_04055"
     CDS             complement(999..2345)
                     /locus_tag="VN647_04055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HWG96238.1"
                     /translation="MKSKPAIHRISEFVGSSLFVRLVKVGLVIAAVVHGYIISFGRSF
                     HFRDIDIHREIGRRFLSGEYLYANDYCYMYLPTTGIYFAPLLILERNASLALRYAVAV
                     GCLVITIMLFHRMLRGTSHSSAWSRLLVGIGAGALTLQFILNDLDDGGPHLMLLGILT
                     GGIYAIWVGKERFGAMLIGLSIVLKITPALFVLLFLWKRQWRLASYTVLATLLWIVLP
                     ILYMGPTSWWNHHMEWTRNAVLSVLDRQMEGRQENELQKANLSLRHTMLRYLVAYPPG
                     HRLRQVDQSYKPVLDLPSPIANVIVGAAGLTLLGLFAWSSRRPYQGVGDPAWARECAG
                     TLLLALLFSPITWDQHLVWMIPAAYVVVAAAAKMSGGLSRTGYVMLAVYVVLTMVLNY
                     EVVGSARWEALKSYHHLGIALLILYGLLLSTKRSWSTPNPHPEKILSGFPRMAGGP"
     gene            complement(2456..>3542)
                     /gene="ispG"
                     /locus_tag="VN647_04060"
                     /gene_synonym="gcpE"
     CDS             complement(2456..>3542)
                     /gene="ispG"
                     /locus_tag="VN647_04060"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWG96239.1"
                     /translation="TRDVAATVAQIHQLEAAGCELIRVAVPDDEAARALPHIKSAMTV
                     PLIADIHFDHRLALKAADIVDCVRINPGNIGAWWKVEEVIKAVNERRIPLRVGVNGGS
                     LERPLLEKYGWPSPEALAESALNAVHALEDVGFTNMKVSLKASDVHHAIDAYWLFAHQ
                     SDYPLHIGITEAGTAMTGAVKSSIGLGYLLSQGIGDTLRVSLAADPVEEVKVGFEILK
                     SLELRHRGINVIACPTCGRVEIDVVRMANELEKKLNHIKTPLNVSVLGCVVNGIGEGK
                     EADIGIAGGEGKGILFKKGKLVRKVPMEELMDTLIVEVEQLAKEKEAESNGEASASVS
                     SNGWESMEPQSDHPSTLGKEIPVLPLR"
BASE COUNT          836 a         1026 c          958 g          722 t
ORIGIN      
        1 ggcgcagcat gattatgaaa agcctggtgc tgggaatcgg atcactattt cttcttgcgt
       61 tcgtttattt gggaatctcg tccggcgcca aggatgccgg gggcattcct gccgagaaag
      121 tggcggatat gttgcattcc gtcatcgagt caaaccgcac attttatacg cagcatgttg
      181 tagagcggat gcaagcgaaa ggcgctgccg tcgcatcgga gaattggcgc tcagaaaaga
      241 ccttacccct tccagcccag ttcttacagg aaagcgctcg tctatcggtc aattcgaagg
      301 ctaaggtcgg ctacaaactc atcagtctct ggcccatcaa caagcagaac ggggccaaat
      361 ccgaagctga gcataggggc ctgatggcaa cccaacagaa cccagaccgc ccgtacactg
      421 aagtggtcat gaacggagcg gatgcctatc tcatggccgt ctatgccgat aaagctgttt
      481 cgcaagcttg catcggatgc cacaacgcgc atcaggatag tccgagacac gatttcaagc
      541 agaatgacgt gatgggtgcc atcatgatca cgatcccgct gaacctctag cggcacgagc
      601 aagcgaggta tccgaaagaa gacacccggc gatgattatc ccgcggcgtc aattgaagag
      661 aggctacgcc tgaagagccg cgggtacgca tgaaggaaga attagagcct actggtggaa
      721 aagtacctat ccgccgcgat ttgatcgaac gcggcggcac gagcgagctt ggtttggagg
      781 cgccgagcgg gttcgaaccg ctgcatcgca gttttgcaga ctgctccctt accacttggg
      841 tacggcgccc cgggaggcgg cattatagtc gcttcctttt ccagaactca acacaagatc
      901 gacccaacaa accgatctcg tcggaattgt ttcgagcggc aagagcattg tggcaattgc
      961 tgttggacgg agcgatcaaa cggaccggca acaatgcgct atggcccgcc ggccatgcga
     1021 ggaaagccgg aaagaatttt ttccggatgc ggattcggag tactccagct gcgcttagtg
     1081 ctgagaagta atccgtacaa gatcagcagc gcgataccga gatgatggta actcttgagc
     1141 gcctcccatc tggctgaacc cacaacttca taattcagta ccatggtcag cacaacgtac
     1201 accgccagca tcacgtatcc ggtacggctc aacccgccgc tcatttttgc agcggcagcc
     1261 acgacgacat aggccgccgg tatcatccac accaaatgct gatcccaggt aataggagaa
     1321 aacaataagg ccaagagcaa cgtaccggcg cactctcgcg cccacgcggg atctcccacc
     1381 ccttgatatg gccgccggct ggaccaggcg aacaggccga gcagagtcag cccggccgcc
     1441 ccgacgatca cgttcgcaat cggcgacgga agatccagca ccggcttgta gctctggtcc
     1501 acttgccgaa ggcgatggcc cggcgggtac gcaaccagat accgaagcat ggtatggcgg
     1561 agtgaaagat tggccttctg aagttcattt tcttgcctgc cctccatctg ccgatcaagc
     1621 acggataaca ccgcgttcct ggtccattcc atgtgatgat tccaccaact cgtgggtccc
     1681 atatataaga tgggcagtac gatccacaag agggtcgcga gcaccgtgta tgaggccaat
     1741 cgccactgcc gcttccacag aaacaacagc acgaaaagcg cgggggtgat cttgagaacg
     1801 atgctgagtc cgatcaacat cgcaccgaac cgttctttcc cgacccatat cgcgtaaatt
     1861 ccaccggtta ggatccccag tagcatcagg tgcggcccgc cgtcgtcgag atcattcaag
     1921 atgaattgca gcgtcaacgc tcccgcgcct atgccgacca acagccggct ccacgcactg
     1981 gagtgtgatg tgccgcgcag catgcgatgg aacagcatga tggtgatgac taagcatccg
     2041 acggcgacgg cataccgcag cgccaagctt gcgtttctct ctagaatgag caagggagcg
     2101 aaatagatcc cggtggtcgg caaatacatg tagcaatagt cgttggcgta gagatattcg
     2161 ccggagagaa agcgccttcc gatctcgcgg tggatgtcga tgtcacggaa gtgaaaggac
     2221 cgtccgaaag atatgatgta gccgtgcacc accgccgcaa tcacgagacc gaccttcacc
     2281 aacctcacaa acagggaaga tccgacgaac tcactgatcc ggtgaatggc gggctttgat
     2341 ttcatggctc agcggcacac atacaagaac cggtgaaagt gcgtcaaccg tttcttttgc
     2401 aaaggggatt acgggttgaa tgacgtaggt gagaatatgt tcttaacagc gcccgttacc
     2461 tgaggggcaa gaccgggatt tcttttccaa gagtcgaggg atgatctgac tgaggctcca
     2521 tcgattccca cccgtttgaa gagacagagg cactcgcttc gccgttgctt tccgcttctt
     2581 tttccttggc gagttgttcc acttccacaa tgagcgtgtc catcaattct tccatcggca
     2641 cctttcggac aagcttgcct tttttgaaca aaataccctt gccctcgccg ccggcaatcc
     2701 cgatatcggc ttcttttccc tctccgatgc cgttgacgac acaacccagc accgacacgt
     2761 ttaatggagt cttgatgtgg ttgagttttt tctccagctc gttcgccatc cggacgacgt
     2821 cgatttcgac gcggccgcac gtcggacagg cgatgacatt gatgccccga tgacgtaatt
     2881 cgagtgattt caggatctcg aacccgactt tgacttcctc caccggatca gcagccagcg
     2941 acacgcgcaa cgtgtccccg attccctgcg aaagcaagta cccaaggccg atggacgact
     3001 ttaccgctcc ggtcatggcc gtccccgctt cggtaatccc gatgtgaagc ggataatcgg
     3061 actgatgggc gaagagccag taggcatcga tggcatgatg cacgtccgag gctttaagcg
     3121 acaccttcat gttggtaaag cccacgtcct caagcgcatg cacggcattg agcgccgact
     3181 cggccagcgc ctcgggggaa ggccatccgt acttctccaa caacggtcgt tcgagcgacc
     3241 cgccgttgac gccgactcga agcggaatcc ttcgctcatt cacggcctta atgacttctt
     3301 ccaccttcca ccaggcaccg atattgccgg gattgatgcg gacacagtcg acgatgtccg
     3361 ccgccttcaa agcaagccga tgatcaaagt gaatatccgc aatcagcggt accgtcatcg
     3421 ccgatttgat gtgcgggagg gcccgggcgg cctcatcgtc cggaacggcc acacggatga
     3481 gttcgcagcc ggcggcttca agctgatgaa tctgtgcgac cgtcgccgcc acatcgcgtg
     3541 tg
//
LOCUS       DATNDD010000171         7799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_58105, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000171 DATNDD010000000
VERSION     DATNDD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58105"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..2681
                     /locus_tag="VN647_04065"
     CDS             45..2681
                     /locus_tag="VN647_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWG96240.1"
                     /translation="MEVSLTPMPAFLRVLLLLLTSHVRQWPLRTLLTIVGVALGVSAS
                     VAVRTANVDVLRSFEQTVMTVAGPTTLDVSGGEAGFDEHIITKVRTVVGVTSASPMIL
                     QTAVRMRGEQADQAVQVVGLDLLAEFNTRGFRLSQPASESQLMSMIQPQAVFLGAKLA
                     AEWNLSVDDPVDLLIGPRRLTCRVAGILHNESDRTASWERMAVMDIAAAQITFGMVGK
                     VDRIDIVTDEKRAIEDVAQDLRTVLPPHMTVERPANRTSQVEQMIRAFRLNLTVLSWV
                     GLLVGIFLIYNTMAFAVAQRRREIGIYRAIGMTQSRVAVLFLVEAGLFGFLGGIAGSG
                     AGVALAQELVALLSRTISDLYAPVGAGEGGLLWAGQFWRIMIEGIVIGCVVSMIGAVG
                     PSLDAGRTTTVRALAPGDYEASRQLRVGMLGVAGLSLLAAAGLLSLPGPIGEVPVLGY
                     AATLCLLAGLACLAPICVTGWRRPRRYVGREFGVRGVMRGIAVEHASRSPGRNGVTVS
                     ALMVGLAIMIGVLVMVRSFRHTVELWVTDTVLADLVVAPSMWLRGTEIGSAGRSLPPM
                     WLGVLSSIPEVAAVDSYRDVRVEVNGQRVAVVSRDLRLHAQWSRYLVRKGDSSEQLRR
                     AADTRGLLVSEVLANRLGVEEGSTIEIMTPSGPMQFPIVAVFYDYSTDGGKLLMDRAL
                     YQSLWHDDLVTVFPVYLRDGSSIDRVRERITEQLSGAAGGGLPPLVISNTELRKEILE
                     IFDRTFLLTYVLEAIAVVIAMLGIVNTLVTSVLERRREFATLRAIGGSAEQIRQLVLW
                     EAAYLGVVGIALGLIGGGLLSLLLIKVINKQSFGWTIQMIIPVGALIQAVVLAVIATL
                     VAGYFPARWAARQPIVEGLREE"
     gene            2764..3045
                     /locus_tag="VN647_04070"
     CDS             2764..3045
                     /locus_tag="VN647_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96241.1"
                     /translation="MRIDCVSAQRYGLIVIVGFLCLSVLAQMLGMSGSLIDLLTPSGT
                     AVASVSEDVSLTPLTLEPGTSGLSRLRVLAQPSLQLPVLVTSVFHPPQA"
     gene            3298..4188
                     /locus_tag="VN647_04075"
     CDS             3298..4188
                     /locus_tag="VN647_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96242.1"
                     /translation="MFTLLCVAPFNAQALEKYGHPLPSMKQAGEDSLNAESPLLSGYF
                     LTSVFPYNPSYFARPDNSGLVGMRHMLHLETDLYKQYLTFYTDQNFFSDRTNGWIELS
                     EWDSTYALTGVVDHFNWRIQYERDAGLDRRTVVQEYGDVLVTAKLQAIQDFAWWGQRF
                     PNQNLTVYAGGGWLFHNDNYFARPDNTGRALYRYVVHTDLDLYQNRVVLFGDINMFTD
                     SQSSNKVRPTELDWMVGLALRWQNTEVAVYYEEDHPLDRSGLIQKYLAVQFRLSFDVF
                     KRHLGIGKTPPTTTVGEDQL"
     gene            complement(4419..4817)
                     /locus_tag="VN647_04080"
     CDS             complement(4419..4817)
                     /locus_tag="VN647_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96243.1"
                     /translation="MKTSLRTVPALLLLLTLGCITSPIPVTPEQIASADYGTVPVPPA
                     YQDAIKGYMQEILFDPRTARYRFVGEPQRGYAYLLGRRKPPVFGYLVQVEIDAKNLKG
                     RYTGERSYRFFIKDETLYPLDTSTKTEVVQ"
     gene            complement(5060..5135)
                     /locus_tag="VN647_04085"
     tRNA            complement(5060..5135)
                     /locus_tag="VN647_04085"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5100..5102),aa:Met,seq:cat)
     gene            complement(5156..5530)
                     /locus_tag="VN647_04090"
     CDS             complement(5156..5530)
                     /locus_tag="VN647_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWG96244.1"
                     /translation="MSAQVDHVKALARQLNRLTPYLFPHVTGRFQGERRRDFRKVWHT
                     ALVQVGLVGMLRHDFRRTAVRNMVNLGVPERVAMKITGHQTRAVFDRYHIVSPADLQN
                     TARMLTGTNTGTVGESSLTPAL"
     gene            complement(5560..6705)
                     /locus_tag="VN647_04095"
     CDS             complement(5560..6705)
                     /locus_tag="VN647_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96245.1"
                     /translation="MKKSKQAKEQITPRTMLQIRVVRQDEKGTIRNVTHEDDRGGYGL
                     RGLQMRSSCAYDSLLRTREFLGLLELAVENRDHVDAALVRRVLGDVVTELSRGGDLGC
                     YVQYLQELFEGAMALPWINGTSPQCSTRAICHRGRRITRWLLCREPYNKPKGAHSMGM
                     LYKCGKVFWIKYYSAGRPIRESMGTTKQKEAERFLKDREGRVAMGTPALPRIDRIRYD
                     ELADDLRRHYEVTNCRDLKEADVRLKPLKAFFSGRRASGIGGPDIERYVQVGQGAAVS
                     NGAINRELSTLGRMLRLAYEYGKLQRVPMIHRLKEAAPRQGFFEREQFAAVRKQLPPD
                     LQAAVTIAYTYGWRMQSEVLTLTLSQLDLEAGHFALSQEQQRMPRGG"
     gene            complement(6923..7282)
                     /locus_tag="VN647_04100"
     CDS             complement(6923..7282)
                     /locus_tag="VN647_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96246.1"
                     /translation="MAKKALALVRKGSKQQPEAPTPHHDRKRALSEDWLFQGKDEVGR
                     EGWFLRIEVTGMWPRRCGPFATQDKACEFLEGFLNRHVLGGFCEIQSEMTPPHQTCVT
                     EGVPQLAATPNGAGGSQ"
     gene            complement(7308..7448)
                     /locus_tag="VN647_04105"
     CDS             complement(7308..7448)
                     /locus_tag="VN647_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96247.1"
                     /translation="MKMIRIPVQLPTKLKSKLDAERKRGTSAAGLIRHLLEQYFKGKK
                     AA"
     gene            7558..7749
                     /locus_tag="VN647_04110"
     CDS             7558..7749
                     /locus_tag="VN647_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96248.1"
                     /translation="MTKPQQQTRDYLLRGLPLDVAEKLKVAASLHRASMREYMIEVLE
                     AHLKELERKGITLTLPKGK"
BASE COUNT         1634 a         2043 c         2233 g         1889 t
ORIGIN      
        1 gagggtgcga tcacgcagat ggggactgat aagacatctc caagttggaa gtctctctca
       61 cgcccatgcc cgctttttta agggtgctac ttcttcttct cacctctcat gtccggcaat
      121 ggccgttacg cacgctgctg acgatcgtcg gagtggctct cggagtgtcg gcctccgtag
      181 ccgtacgaac cgccaatgtc gacgtgctac ggtcctttga gcaaacggtc atgacggtgg
      241 ccggtccgac caccttggac gtgtcaggag gtgaggctgg gtttgatgaa cacatcatta
      301 cgaaggtacg aaccgtcgtc ggcgtgacgt ccgcatcgcc gatgatccta cagacggctg
      361 ttcgcatgag aggagagcaa gccgatcaag cggtacaagt ggtgggtctg gatctattgg
      421 ctgaattcaa tacacgcggg tttcgcttga gccaaccggc gtcggaaagt cagctgatga
      481 gtatgattca accacaggcg gtgttcttgg gagccaaatt ggccgctgag tggaatctat
      541 cggttgatga cccggtcgat cttctgattg ggcccaggcg tctcacctgt cgggtggcag
      601 ggattcttca caatgagtcg gaccgaaccg catcatggga acgcatggcc gttatggata
      661 tcgctgccgc acaaatcacg ttcgggatgg tcgggaaggt ggatcgaatc gatatcgtga
      721 ccgatgaaaa aagggcaatc gaagacgtgg cacaggacct acggactgtt ctgcctcccc
      781 atatgacggt ggagcgaccg gccaaccgga cgagccaggt tgagcaaatg atccgcgcct
      841 tccgtctcaa tctaacggtg ttgagctggg tcggcctgct ggtcgggata ttcctgattt
      901 acaacacgat ggcatttgcc gtcgctcaga ggaggcggga aatcggaatt tatcgagcga
      961 tcgggatgac tcagtctcgg gtggcggttc tgtttttggt ggaagcgggg ttgttcggat
     1021 ttttgggagg gatcgccggg agtggagccg gggtggcgct ggctcaagag ctggtggctc
     1081 tgctcagtcg aactatttcg gacctctatg ctccagtggg tgccggcgag ggagggctac
     1141 tctgggccgg ccaattctgg agaatcatga tcgagggaat tgtgatcggg tgcgttgtgt
     1201 ccatgatcgg tgccgtcggt ccaagcctgg atgccggccg cacgacgact gtacgggcgt
     1261 tggctcccgg tgactatgaa gcaagccgtc agctacgggt gggcatgctt ggtgtcgcgg
     1321 gactgagctt gctcgctgcc gctggacttt tgagccttcc tggaccgata ggcgaggttc
     1381 ctgtcttggg ttatgcggct acattatgtc tactggcagg gttagcctgt ctggccccaa
     1441 tttgcgtcac tgggtggcgt cgtccacgcc gatatgtggg gcgtgaattt ggagtacggg
     1501 gagtcatgag ggggatcgcg gtagagcatg catcccgcag tcccggacga aacggagtca
     1561 ccgtctctgc cttgatggtg ggattggcga ttatgatcgg cgtgcttgtg atggtgcgaa
     1621 gttttcgaca tacggtcgaa ctctgggtca ccgatacggt cctggccgat ctggtcgtgg
     1681 cgccttcgat gtggctgcgg ggcacggaga tcgggagcgc cggtcggagt ctcccgccaa
     1741 tgtggttggg cgtcctctcc tccattcctg aagtggctgc ggtcgacagt tatcgcgatg
     1801 tacgggtgga ggtgaacggt caacgggtgg ctgtcgtgtc gcgggatctg cgattgcatg
     1861 ctcaatggag ccggtatctc gtgcgcaagg gcgattcttc ggaacagctg agacgggcgg
     1921 cagatactcg tggtctcttg gtatcggaag tcttggccaa tcggctcggc gtggaagaag
     1981 gatcgacaat tgaaattatg acgccgagcg gtcccatgca attcccgatc gtggccgtgt
     2041 tctatgacta ttcgacggac gggggaaaac ttctcatgga tcgggcactc taccagtcgc
     2101 tctggcacga tgatctggtc acggtattcc ccgtatactt gcgcgacggg tcaagtatcg
     2161 atcgtgtgag agaacggatc acggaacaac tgagcggcgc agccggcggg gggcttccgc
     2221 cgcttgtgat cagtaacact gagttacgga aagaaattct tgaaatcttc gatcgcacat
     2281 ttcttctgac ctacgtgctg gaggccatcg ctgtcgtgat tgccatgctc gggatcgtca
     2341 atacactggt cacgtccgtc ctcgaacgtc gccgtgaatt tgcgaccctg cgggccatcg
     2401 gtggcagcgc ggagcagatt cggcagctcg tgttatggga ggcggcctac ctcggtgtgg
     2461 tggggattgc cctggggctt atcggcggag gcttgctttc tctattactg atcaaggtca
     2521 tcaacaagca atcgttcggg tggacgattc agatgattat tccggtcggt gcgcttattc
     2581 aggccgtggt gcttgcagtc attgcgacct tggtggcggg ctacttccca gcccgctggg
     2641 cagcacggca accgatcgta gaggggttgc gggaagaata atgtggcgat tactttgagt
     2701 ctctaagcag cgcgagaaca ccggctcagc ggggatgtat ggtggttcag aacgagacta
     2761 aacatgagaa ttgattgcgt ttcagcccag aggtatggat tgatcgttat tgtaggattt
     2821 ctgtgcctta gtgtcctcgc gcagatgttg ggaatgtccg gtagcttgat cgatctcctt
     2881 accccatcag gtacagccgt ggcgtccgtt tcagaagatg tttctctcac cccgctgacg
     2941 cttgaaccag gaacatcagg actaagtcgt ctgcgtgtcc tcgctcaacc atcgcttcag
     3001 ctcccggtct tggtgacctc tgtttttcat cctcctcaag catagaattg acggtccctc
     3061 acaattatcg cttctagatt gatttagacc tgtagaacgc accggctttc actggctcaa
     3121 tcttttagtt caaagaagct cgatggagtt gcggatgggc atagcgcagc tgttaggcca
     3181 catggtccca tcaataatat tcgttggagg aatcgagtgt caccacatag tgctaagccc
     3241 tactcccaat tcaggaacgg tggtattcct agcgtctctc cgacattctt aggattcttg
     3301 ttcaccctct tgtgcgtggc accgttcaat gcgcaggcgc tcgaaaaata tggacacccc
     3361 ttgccgtcca tgaaacaggc cggtgaggac agtttgaacg ccgaaagccc gttgttgagt
     3421 gggtatttcc tcaccagtgt gtttccgtac aatccaagct attttgcaag accagacaat
     3481 agcggtctcg taggcatgcg gcacatgctg catttggaaa ccgatcttta taaacagtat
     3541 ctcacgttct acactgacca gaactttttc tcggaccgca cgaacggatg gattgagctg
     3601 agcgaatggg atagtactta tgctttgacc ggtgtggtcg atcactttaa ttggcgcatc
     3661 caatatgaac gcgatgcagg gctggatcgg cgcaccgttg ttcaggaata cggcgatgta
     3721 ttagtgacgg ccaagctcca agcgattcag gacttcgcat ggtggggaca acgctttccg
     3781 aaccagaatc tcacagtcta tgcgggtggc ggatggttgt tccacaatga caattacttt
     3841 gccagacccg acaataccgg acgggcactc tatcgttatg tggtccacac ggacctcgat
     3901 ctctatcaga accgggtggt cctgtttgga gacatcaaca tgttcacgga tagccaatcc
     3961 agtaacaagg tgaggccgac ggaactcgat tggatggtcg gcctggccct tcgatggcag
     4021 aacacggaag tcgccgtgta ctacgaggag gaccatccct tagatcgatc ggggctgatt
     4081 cagaaatatc tggctgttca gttccgcttg tctttcgatg tgttcaaacg gcacttggga
     4141 atcgggaaga ctccaccaac caccaccgtt ggagaagatc aattgtaatg atcatatgca
     4201 ttcgcaaata actaaaactg aaaacgattc attgcctgga tcagcttgac ctgcagctta
     4261 tcagggacta agtaggactc cgtggagggg ggagccggca ctcgactgtc acaaaaagtg
     4321 ttctcctgta aaaagaactc gcttcctgcc tcacgatcag ggtcctttca gattcctcgc
     4381 gtcatgagag atttggaagt agactctggc aatagagcct actgcaccac ttcggttttg
     4441 gtggaggtat ccaagggata cagcgtttcg tccttgatga agaatcgata cgatcgctcg
     4501 cctgtgtagc ggcccttcag gttcttcgca tcgatctcca cctgcacgag gtacccaaac
     4561 accgggggct tcctcctacc taaaagatac gcgtagcctc tctgtggctc tccgacgaat
     4621 cgatagcgag cggtgcgcgg atcaaacaga atctcttgca tgtagccttt aatggcatcc
     4681 tgataggctg gtggcactgg aacggttcca tagtcagcac tcgcgatctg ttcaggagtg
     4741 acgggtatgg gagacgtgat gcaaccgagg gtgaggagca ggagcagtgc agggacggtt
     4801 ctgagagagg tcttcaacgg agagataatc gctcctcact gcatatgcgg gccgcttctt
     4861 tgtcgctgcc cggcgctatt cgctattcta ggaaccggtg tgttgatctt caataaggtt
     4921 catcgcccga tgaacagccg actcagaaga tatagaaccg tgagtgaggt ttggagtaga
     4981 ggaaagtcaa agaggtttat ccgccggcat caatcacaaa aaaacgaaaa ttagccggcg
     5041 ggtcaaccga aggttggtat ggtggcggtg ggccggatcg aacggccgac ccgcggctta
     5101 tgagtccgct gctctgccaa ctgagctaca ccgccacgcc aaagaaatta acgagttaca
     5161 gtgcaggagt caaggaggat tcgcccactg tgcccgtgtt tgtgcccgtg agcattcgcg
     5221 cagtgttttg gagatccgct ggactcacaa tatggtagcg atcaaacacc gctcgcgttt
     5281 ggtggccggt gatcttcatg gcgacccgtt caggcacacc aagattgacc atattgcgga
     5341 cggcggtccg gcggaaatca tggcgcaaca tccccacaag ccccacctgt accaaggcgg
     5401 tgtgccagac cttgcgaaag tcccggcggc gttccccttg aaacctgcct gtcacgtgcg
     5461 gaaagagata aggggtgagg cgattcagtt gtcgcgctaa ggctttcacg tgatcgactt
     5521 gggcactcat gagcgcctgc agttcgggcg tgagatagac tagccgcccc tcggcattct
     5581 ttgttgttcc tggctcaagg cgaagtggcc tgcctcaaga tcaagttgcg acaaggtcaa
     5641 cgtcaggact tcgctctgca tgcgccagcc gtaggtatac gcaatcgtga ccgccgcttg
     5701 cagatctggg ggaagttgct ttcgaacggc tgcaaactgc tctcgctcaa agaaaccttg
     5761 acgaggagca gcttctttca ggcgatgaat cattggtaca cgctgcagtt ttccatactc
     5821 gtaggctaac cgtaacatgc gtcccaatgt ggagagttcc cgattaatgg ccccgttact
     5881 gactgccgca ccttgcccaa cttgaacata gcgctcaatg tccggtccgc caattcctga
     5941 ggctcggcgc ccgctaaaga atgccttgag cggcttgagt cgaacgtctg cctctttcag
     6001 gtctcggcag ttagtcactt cataatggcg gcgaagatca tccgccagtt catcgtagcg
     6061 tatgcggtca atccgtggca gggcaggggt ccccattgcg acccggcctt cacggtcttt
     6121 caagaaccgt tcagcctcct tctgtttggt cgtccccata ctctcccgaa tcggacgccc
     6181 tgccgaatag tatttgatcc agaacacctt cccgcatttg tacagcatgc ccatactgtg
     6241 tgctcctttt ggcttattgt atggttcacg acacaagagc cagcgagtga tccgtcttcc
     6301 tcggtgacaa atagctcttg tcgaacactg cggcgaggtc ccgttaatcc aaggcaatgc
     6361 catcgcgccc tcgaagagtt cttgcagata ctgcacatag catcccagat cgccaccgcg
     6421 agataactct gtcaccacat cgcccagtac gcgccgcacc agcgcagcat ccacatggtc
     6481 cctgttctcc actgcgagtt cgagcaaccc tagaaattct ctcgtgcgta ggaggctgtc
     6541 gtatgcacat gagcttctca tctgaagacc ccgcagccca tacccgccgc gatcgtcctc
     6601 gtgcgtcaca ttgcgaatgg tgcccttctc atcctgacgc acgacccgga tctgcagcat
     6661 ggtccgaggt gtgatctgct cttttgcctg tttgctcttt ttcatgtcga tcctcccttt
     6721 tgacggtgat tgtgaattgg tcagttgctc tgcctgcggc tctttggctt tcgtgcctgc
     6781 gcgagcagat actccaggcg atccatgccg atggctttgc ccagctttga ttgcaaccgc
     6841 aatctccgtc ctctcctgat ctgcccacca acgatggcgg tgcgggagtg gccattcgcc
     6901 ggttgacgaa attgcttcat gttcattgcg agccccccgc tccattgggc gtcgcggcta
     6961 actgaggtac gccttcggtc acacatgttt gatgaggtgg ggtcatctcg ctttggattt
     7021 cgcagaagcc tcccagcacg tgcctgttca ggaacccctc tagaaattca cacgccttgt
     7081 cttgtgtcgc gaatggcccg catcgtcgcg gccacatgcc ggtgacctca atgcggagaa
     7141 accacccctc gcgcccaacc tcatccttgc cctgaaacag ccagtcctcc gatagagcgc
     7201 gcttacggtc gtgatggggg gttggtgcct cgggctgttg tttggaacct ttgcgaacga
     7261 gtgccaatgc tttctttgcc attgagtcct ccttggttgg ttgtgaacta ggcggccttc
     7321 ttgcccttga agtactgctc cagcaaatgc cgaatgagtc ccgccgcgct ggtgccgcgt
     7381 ttccgttcgg cgtcgagctt ggatttgagt tttgtgggta gctgaacagg gatgcgtatc
     7441 attttcataa aacctccaat gttattaaga taataatact cagctaaata ctaattgata
     7501 tcagtatatc aatagatatt tattgatgtc aaggtgtcaa tttgatacac tcgcggcgtg
     7561 accaaacccc agcaacagac ccgtgactac ttactgcggg gcctgccttt ggatgtagcc
     7621 gaaaagctta aggttgcggc ctcgcttcat cgcgcctcta tgagggagta catgattgaa
     7681 gtcttagagg ctcatctcaa ggaattggaa cggaagggga ttacgttaac gctgccgaag
     7741 ggaaagtaac cagaagcgct aagctcacag cggtatgcga atacacgtta gcagattga
//
LOCUS       DATNDD010000172         2798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2044791, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000172 DATNDD010000000
VERSION     DATNDD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044791"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VN647_04115"
     CDS             complement(<1..393)
                     /locus_tag="VN647_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWG96249.1"
                     /translation="MYPVVRCALDTMFVMVVTTTALGMFSVVYAQNGEQAQTIKGVVQ
                     NQDLRRVPQAVIEVRSQDGEIISSAVSNDAGEFKITVPEGGTYSVSAVQETYRSEYAV
                     VTIGKERPAPVTLTLALTKEIALEIVSPL"
     gene            complement(505..774)
                     /locus_tag="VN647_04120"
     CDS             complement(505..774)
                     /locus_tag="VN647_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96250.1"
                     /translation="MISIILGLAPFAVAQDVHHELAAADSDGHEHSDTDICQWVQHHI
                     AGSVDLDLPRLTVCSVARHQELPSESILLSAAPSLVGPSRAPPQV"
     gene            1010..2071
                     /locus_tag="VN647_04125"
     CDS             1010..2071
                     /locus_tag="VN647_04125"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWG96251.1"
                     /translation="MNRPIDNQHVIEIKALPSPRAIKTKLPITDQAAALVVETRESIR
                     RILHGKDRDRLLVIVGPCSIHDPDAAYEYAVKLKPVADALRDRLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIATGMELAREILLKINQLGLPCGTELLDPISPQYVADLISW
                     AAIGARTTESQTHREMASGVSMPVGFKNGTEGSLQVAVNAMISARTLHHFVGINADGQ
                     TAIIRTMGNPDRHIVLRGGGGRTNYEEEHVARTEAAVAGEGIARPIMIDCSHDNSKKD
                     HTRQGLVAHEVLRQFRGGRQTIMGLMLESNLNPGRQAWKEDVALLHGVSITDACLGWD
                     ETERLLTELAAAITPKPVS"
     gene            complement(2134..2385)
                     /locus_tag="VN647_04130"
     CDS             complement(2134..2385)
                     /locus_tag="VN647_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96252.1"
                     /translation="MTKSAAIEAFEICMIVSAVTVASGAWGSALAGMALALLGTGCVV
                     IVAKGTLQHVPEVDLKLWTGVLLSSIGLWWLYEASVNWP"
BASE COUNT          659 a          828 c          747 g          564 t
ORIGIN      
        1 cagcggagag acgatttcca gcgcaatctc cttcgtcagg gccaatgtca gggtcactgg
       61 agcaggtcgc tccttcccaa tagtcaccac tgcatactcg ctccgatatg tctcctgcac
      121 ggcgctgacg gagtacgtgc ccccttcggg gacagtaatc ttgaattcac ccgcatcgtt
      181 cgagaccgcg gacgatatga tttcgccgtc ttgactcctc acttcgatga ccgcctgcgg
      241 cacgcgccga agatcctggt tttgtacaac ccccttgatt gtctgggctt gttcgccatt
      301 ctgtgcataa acgaccgaaa acattcctaa tgcagttgtc gtaaccacca ttacgaacat
      361 cgtgtccaac gcacatctca cgactgggta cattatgccc ctcctcactc acaagtcagc
      421 gattaacaaa tgaaaattga taggataggc atacatggac ttcacggcct atcaaccgtc
      481 ctgactgctt gacgtgatgt tcggttatac ttgaggagga gcgcgggaag gcccgacaag
      541 cgatggggcc gccgaaagca gaatggattc agaaggaagt tcctggtgcc gggcaacgct
      601 gcacacggtt aatcgtggaa gatcgagatc gaccgagccg gcgatatggt gctggaccca
      661 ctggcagata tcggtgtccg aatgttcgtg accatccgaa tcggccgcgg ctaactcgtg
      721 gtggacatcc tgggctacgg cgaacggagc cagcccaagg atgatagaga tcatccccaa
      781 cgcgaccccc gaacggagca acgaagagag atatctgaac cttattggga acatggtcgt
      841 agaataggca cccatggtca gaattgcaag aacagccgga tgggcgtgcc cccatcagct
      901 cgattcccgg acgggtcact cggtatggac ttttccggca tgattcgtta tattaccacc
      961 cccctcaagt tctcaacgag aacgagatct ctgaaggaca taccccttca tgaataggcc
     1021 gatcgacaat cagcatgtta ttgaaatcaa ggcgttgccg tctccgcgcg ccattaaaac
     1081 caaactgcct attaccgatc aggctgcggc actcgtcgtc gaaacccgag aatcgattcg
     1141 ccgaattctc catggaaagg atcgtgacag gcttctggtc atcgtgggac cgtgctccat
     1201 ccatgacccc gatgccgcct atgagtatgc cgtcaagttg aagccggttg ccgatgccct
     1261 gcgtgaccgt cttctgatcg tgatgcggac gtactttgaa aaaccacgaa ccaccgtcgg
     1321 atggaagggt ctgattaatg acccgcatct cgacggtacg tgcgatatcg caacaggaat
     1381 ggagttagcc agggagattc tcctcaagat caatcaattg ggtcttcctt gcggaacgga
     1441 gctgctggat ccgatcagtc cacaatacgt cgccgacctc atcagttggg cggcgatcgg
     1501 ggctcgtacc acggaaagcc aaacccatcg agaaatggcg agcggcgtgt ccatgccggt
     1561 cggcttcaag aacggcactg agggcagctt gcaggttgcc gtcaatgcca tgatttccgc
     1621 ccgcacatta caccattttg tcggcatcaa cgccgacggt caaactgcga tcatcagaac
     1681 catgggcaac cccgaccggc atatcgttct ccgtggcgga gggggaagga cgaactacga
     1741 ggaggaacat gtcgctcgga ctgaagccgc ggtcgccgga gaaggcatcg cccgtccgat
     1801 catgatcgat tgttcgcatg ataattccaa gaaagatcac acacgccaag gtttggtcgc
     1861 ccatgaggtt ctgcggcagt tccgtggagg tcgccaaacc atcatgggtc tcatgctcga
     1921 aagcaacctc aatcccggca gacaggcatg gaaagaagat gtagccctcc ttcatggcgt
     1981 ctcaattacc gatgcctgtc tgggctggga cgagaccgaa cgtctgttga ccgaacttgc
     2041 cgcagcaatt actccgaagc cggtttcctg aaccctattc tttccgacca ttgtagctcg
     2101 gagcaccggc cgcttgttcg ggtcagcagc ctctcacggc caattcaccg acgcttcgta
     2161 tagccaccag aggccgatcg acgaaagcag caccccggtc catagtttga ggtcaacctc
     2221 cggcacgtgc tgaagtgtgc cttttgcgac aatcaccacg catccggttc ccagcaacgc
     2281 cagggccatt cccgccaatg cggaacccca cgccccggac gccacagtca ccgccgaaac
     2341 gatcatgcaa atttcgaaag cctcgatcgc ggcgcttttc gtcatgacaa aggtcgtgaa
     2401 gaagttgaac ctcgttccac tcgattcgct gcagcttgga ccccccgtag aggatcgtcg
     2461 atccaccccg accgttgctt gtaccgcttc cggcgaacgg ccttgatcaa ccacgaccca
     2521 ccgagccaga acaagaggac gccgacactt ggaggttcat agagcgagtg acggagtgcc
     2581 tccatcatga gcatcgcccg cgacttccgc gccgggctgg aacggtacat cgagaagtat
     2641 cagattcaaa gcgtcacagt cgaagacgac ctcgcccgga gacagtacat gaggcggtag
     2701 atgcagtgcg atcgtttatg acgacttcgc ccgggtggac catagtccac cggcgatcaa
     2761 cacaagcgtc agcgcctgcg gcacgagact ctgcgcgg
//
LOCUS       DATNDD010000173         5532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2202362, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000173 DATNDD010000000
VERSION     DATNDD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202362"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VN647_04135"
     CDS             complement(<1..590)
                     /locus_tag="VN647_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2172 domain-containing protein"
                     /protein_id="HWG96253.1"
                     /translation="MAHISQERRIAIMTSVGSKEAVRGEELGKEMHDFMVELYPICRS
                     ITGEGVRETLRAIRKRIPLEMREVSSGTKVFDWTVPLEWNVTDAYVMNREGKRVIDFK
                     AHNLHLMSYSLPVKKKMMLADLRPHLFTLPEHPEWIPYRTSYYKENWGFCLRHADLER
                     LPDDEYEVVIDSSLRPGSLTYGELYVPGEISDEILVS"
     gene            complement(994..2229)
                     /locus_tag="VN647_04140"
     CDS             complement(994..2229)
                     /locus_tag="VN647_04140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96254.1"
                     /translation="MGQSGCRSCGAMLERTFVDLGMSPLANSYIKPDQLNRMESFYPL
                     HVYVCEKCLLVQLQQFSTPHDIFSDYAYFSSFSDSWLAHAKAYVDMIVGRFRLDRSSK
                     VVEIASNDGYLLQNFVSRGIPVLGVEPASNIAEVARKKGIHTKVAFFGEKTALDLAAD
                     GWGADLIIGNNVLAHVPDLNDFVNGLKVLLKPTGLITMEFPHLLQLMQQNQFDTIYHE
                     HFSYFSFLAVEQIFARHGMKLFDVEELSTHGGSLRIYACHDDDVSKPIEMRARELKSR
                     EEIAGFGQLNHYLSFGLQVEVIKRKLLSFLISSKQEGKHVVGYGAPAKGNTLLNYCGV
                     RMDLIDYTVDRNPHKQGHFLPGVHIPIYGPERVRDTRPDYLLILPWNIREEVMQQMIY
                     IREWGGKFVVPIPEVQVYS"
     gene            complement(2302..3360)
                     /locus_tag="VN647_04145"
     CDS             complement(2302..3360)
                     /locus_tag="VN647_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG96255.1"
                     /translation="MKILVTGNMGYVGPLVLRRLRESHPQATLIGYDMGYFAHCLTGT
                     SRFPESLADQQYFGDIRHVPKQILQGIDIIVHLCAISNDPMGATFEKVTLDINHRASI
                     DLAGKARQAGVKKFVFASSCSVYGFAEGGPRREEDALNPLTAYAKSKVQTEQDLASLA
                     SEAFTATCLRFATACGMSDRLRLDLVLNDFVAGALVSKRINILSDGTPWRPLIHVKDM
                     ARAIDWAVQRDSRDGGSFLTINVGSDAWNYQVKDLAAAVANLVPNIEVSINKDAQPDK
                     RSYRVNFDKFSKLAQGFLPMVDLQSAVKDLRDGLVVMQFRDHEFRTSELIRLVTLKRL
                     RESGQLTDDLVWKERSNV"
     gene            complement(3608..4381)
                     /gene="rfbF"
                     /locus_tag="VN647_04150"
     CDS             complement(3608..4381)
                     /gene="rfbF"
                     /locus_tag="VN647_04150"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HWG96256.1"
                     /translation="MKAVILAGGWGTRLSEETTLRPKPMVEIGGKPILWHIMKIYAAH
                     GIKEFIIGLGYKGEMIKDYFLNFYAFNKDISVDLESGKTTIHDGRRHEDWKVHLVDTG
                     LHTQTGGRLKQLKKWIGENETFLFTYGDGVSDVDIQATIKFHTSHGKLATVTTVLPPA
                     RFGRLVFDQDHIVDFKEKPHGEEGWINGGFYVLEYKAIDYIKGDETVWERDPIQQLTK
                     DNQLMGYRHDRFWSCMDTLKEKNYLEELWQSSKAPWKVW"
BASE COUNT         1432 a         1526 c         1182 g         1392 t
ORIGIN      
        1 gagacgagaa tctcatcaga aatttcacct ggtacgtaga gctccccata cgtaagagaa
       61 ccgggccgaa gactcgaatc gataacgacc tcgtactcgt catcaggtag tcgttcgaga
      121 tctgcatgtc gaagacaaaa cccccagttc tccttgtaat aggaggttcg gtacggaatc
      181 cactcaggat gttccggcag cgtgaataaa tggggtctga ggtccgccaa catcatcttt
      241 tttttaacgg gcaaactgta gctcattaga tgtagattgt gcgctttgaa atcgatgact
      301 cgtttcccct cccggttcat gacataggca tcggttacgt tccactccaa cggaaccgtc
      361 caatcaaaca ccttggttcc gctagaaacc tcacgcattt caagtggaat acgttttcgg
      421 atcgctcgga gagtttctct gacaccctca ccggtaatac tccggcatat cggataaagc
      481 tctaccatga agtcatgcat ctctttgcct aactcttcac ctctcactgc ctctttagaa
      541 ccgactgatg tcatgatagc aatgcgccgt tcttgggaaa tgtgggccat gtgcgatcct
      601 tactccgaca tgaccaaaat aggtgcgggc caggcaatct tgaacgcagg gtcatcccat
      661 cgaaacccgc gagggcttgc aggcacatga acctccgaca tgcagtacga caccctcgct
      721 attttgttcg agtgtgagaa acccatgggc acaacaatag ttcctgccgt gcaccaaacc
      781 aacttcactt cctcatgtgg agcacattga tagtgaagcc cacgccgggt gcctttccgt
      841 gtattcaccg aaagcttgac tgtacccatg tgatcgaagt ccatgaacct cgaactcccg
      901 ttgacaccag gtcctcataa acattctccc gctcatctcg caccggttca agatcgacca
      961 ggaatacacc ctggagtgag gtttccgtaa aaatcatgaa tatacttgca cttcaggaat
     1021 cggaacgaca aacttccctc cccactcccg aatgtagatc atctgctgca tcacttcttc
     1081 ccgaatattc caggggagaa tcagaagata atcagggcgt gtgtcacgaa cccgttccgg
     1141 cccataaatc gggatatgca cgccgggcag aaaatgccct tgcttatgtg gattccggtc
     1201 taccgtgtag tcaataaggt ccatccgaac cccacaataa ttgaggagcg tattcccttt
     1261 tgcaggagcc ccgtaaccga caacatgctt gccttcctgt ttcgaagaga tgagaaagga
     1321 cagcaacttt ctcttgatca cctcaacttg cagaccaaac gacaggtagt gattcagctg
     1381 accgaacccg gcgatttctt ctcgagattt cagttccctt gcccgcatct ctatgggctt
     1441 ggaaacatcg tcatcgtggc acgcatagat cctcagagac ccaccatgag tcgatagttc
     1501 ctctacatcg aacagtttca ttccatgacg agcaaatatc tgctcgaccg ctaagaaaga
     1561 aaaataggaa aaatgttcat gatagatggt atcaaactgg ttctgctgca tcagctgaag
     1621 caggtgcggg aattccatgg tgatcaggcc cgtcggcttc agcagcacct tgagcccatt
     1681 cacaaaatcg ttcagatcag ggacatgggc cagcacgttg ttcccgataa tcagatcagc
     1741 accccatccg tcagcagcta aatccagagc cgtcttctct ccgaagaagg ccaccttcgt
     1801 atgaatcccc ttcttcctcg ccacctcagc tatattggaa gcaggctcca cgcccagtac
     1861 aggaatgccc cgagaaacga agttctgcaa aagatatccg tcgttgctgg cgatctccac
     1921 tactttagag ctgcgatcca atctgaatcg acctacgatc atgtcgacat aggcttttgc
     1981 gtgtgccagc caggaatccg aaaatgatga aaaatatgcg tagtcggaaa aaatatcgtg
     2041 tggcgttgag aattgctgca actgaacgag taaacacttc tcgcaaacat acacatgcaa
     2101 tggatagaaa gactccatac gattcaactg gtccggctta atgtaggaat tggccaatgg
     2161 agacatacca aggtccacga aggtacgctc aagcatcgct ccgcaagacc gacatcctga
     2221 ttgccccata cagatatttc ccccttctgg cgaagaatga tgcgtcgtgt tcaatacctg
     2281 acgcgacaac cgaccgcgcc ctcaaacatt gcttcgctct ttccacacaa ggtcgtctgt
     2341 cagttgccca ctctcccgca atcgttttaa ggtgactagt cgtatcaact cactcgtcct
     2401 gaactcatga tctcgaaatt gcattaccac aagtccatca cggagatcct tcacggcact
     2461 ctgaaggtca accataggga gaaatccttg cgccagctta gagaacttgt caaaatttac
     2521 acgatatgag cgtttatctg gttgcgcatc tttattgatc gaaacctcga tgttcggcac
     2581 aagattggct acagctgccg ccaaatcctt tacctgataa ttccatgcgt cgcttcccac
     2641 gtttattgtc agaaaggatc caccatctcg actgtctcgc tgcacggccc aatcgattgc
     2701 tctcgccata tctttgacgt gaatcagtgg gcgccatggt gtcccatcac tcaggatatt
     2761 gatgcgtttt gacacaagcg cgcccgccac aaaatcattc aacacaagat ccaatcgcag
     2821 ccgatcactc atgccgcatg ctgttgcaaa ccgcaaacag gtcgccgtga aggcttccga
     2881 tgcaagcgat gccaggtctt gctcagtttg aactttcgat ttggcatatg ccgtaagagg
     2941 attcaaagca tcctcttctc ggcggggccc accttcggca aatccataca cactgcagct
     3001 cgatgcgaac acaaatttct taacacctgc ttgccttgct ttcccggcca gatcgatact
     3061 tgctcgatgg ttgatatcga gggtaacttt ttcaaaagtg gcgcccatag gatcgttcga
     3121 aatagcgcag agatgtacga tgatatctat cccttgcagg atctgcttgg gaacatgtcg
     3181 gatatctccg aaatattgct gatcggccag actttctggg aatcgcgaag tacctgttaa
     3241 acaatgcgca aagtaaccca tatcgtaacc tatgagtgtt gcctgaggat gggattctcg
     3301 caaacgacgt agtaccaatg ggccgacata gcccatgttg cctgtaacca gaatcttcat
     3361 gccttaactc cctgattaac cgccctgttt ttcagcagac atatcttgag ttgagttaag
     3421 taagcactca caggaatacc gccagacagc attcgccaaa cgcgtcgaat gctaggcacc
     3481 ctcaagctaa attagctccc agctttcccg tcctgatata actgctgtag gaaagccaca
     3541 gactttccca ctccttcaag cagtcacatg attaagcaag agaatacaaa atggtcggga
     3601 atcaatctta ccataccttc cagggggcct tcgacgattg ccatagctct tccaaataat
     3661 tcttctcttt cagagtatcc atacaggacc agaaacgatc atgccgatag cccatcaatt
     3721 gattatcttt cgtgagctgc tggattggat ccctctccca cacagtctca tcacccttaa
     3781 tataatcaat ggccttatac tcgagaacat aaaaaccgcc attgatccag ccttcctcgc
     3841 catgaggttt ttccttgaag tcgacgatgt gatcttgatc gaacacaagc cgtccgaatc
     3901 tagcaggagg caggaccgtc gtcaccgtgg ctaactttcc gtgagacgtg tggaatttaa
     3961 tagtggcttg aatgtcgaca tctgaaaccc catctccata cgtaaataaa aacgtttcgt
     4021 tttcgcctat ccattttttc agctgtttaa gccgacctcc cgtctgagtg tgcagaccgg
     4081 tatcgacaag atgcactttc caatcttcgt gccgtcttcc atcatgaatc gttgtctttc
     4141 cactttcaag atcgaccgaa atatctttat tgaaagcata gaagttgaga aaatagtcct
     4201 tgatcatctc tcccttgtag ccaagaccga taataaattc ctttatgccg tgagccgcat
     4261 aaatcttcat gatatgccac aggatcggtt ttccaccgat ttccaccata ggcttcggtc
     4321 gcagagtggt ctcttcggat agtcgagttc cccatcctcc tgcaagaatt actgccttca
     4381 tgctcatttc ctccttgcat caggccccaa cacgtggaaa gaattcccat cgaagccttt
     4441 ttgaagaacc gataatcgga atctacggag ttcacgctgc agcatatgga atgagttggt
     4501 tgatctccca acacaacccg cgcctggagc gcgagtgtgc gaagaagcac caccgcccgt
     4561 tgatgtccgg acatctctct cataaacacg gcctcaagtc cgacgaatgg gccatcctta
     4621 atttgaacaa gctggccgct acttagctct ttcggcttct ccagtatata cacttgagag
     4681 cttgccattc gcctcttgat ggcatcaatc atcgcgatat cgacctccac aggagtcgca
     4741 ccgaacttca cgattctccg aactcctctg gcataagaca ccgctcgata gtgctccgat
     4801 aaattgattc ggacgaatag atattccggg aagagaggag caattacggt tctgaccttg
     4861 cgtcgaatag tcctctgttc ttgcagcagt ggcaagaaac actccactcc catgtgtcgg
     4921 atattctgct cggcttgttt ttcgtggttt gactttgtac aaagtacgta ccatcgcatc
     4981 tcgcagcgtc tcaatctctc tgaaagagaa tcgctcatag ctcagtgatc taaagcctca
     5041 ataggctacc gcagtccccg tacccaccgg atctttaact ttctaataaa ctatgcgcac
     5101 taagtcaact tgctatgaga aagcggtctt tgcaagatag cagtaattct tcttagagaa
     5161 gccttgaccg acgctccgcc ggcgcgaact gctcacacga tatggcagcc agagtacctg
     5221 tggacgacgt tgacgacggc tatggcaata ggactccagc ggggtgcttc ttgccctctg
     5281 gcgggcgttt gtggtcaagc taaatgccca ccagaacctg cgctggaacg aatgctccac
     5341 atggcctgtg ccctcatcac gctccgcaat attttaaaat gacctcggga gacatcgctt
     5401 ctttctgcga aagtagcagc tgcagataat tatgttcgtc agctcaatcg cttctcaaag
     5461 tctagccgtt cgactcctaa acagagatcc atccatcgca atcaatcgtt tgtcaatatc
     5521 ctacttgtag ga
//
LOCUS       DATNDD010000174         3595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1213193, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000174 DATNDD010000000
VERSION     DATNDD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213193"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN647_04155"
     CDS             <1..223
                     /locus_tag="VN647_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWG96257.1"
                     /translation="SSPYRSVLRSLLDYVEEVLEKDSNTWITVVIPEILPARWWQNIL
                     HNQRALMLKASLLFKDRVILIDVPYHLTR"
     gene            355..1284
                     /locus_tag="VN647_04160"
     CDS             355..1284
                     /locus_tag="VN647_04160"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWG96258.1"
                     /translation="MKLSAGSTVTTHVDLGKDTGIVKVRYYWYREQDEVLVEQDDSTA
                     TGSIVAPVAMVGSAEQEPAFGGSLKVPEDGIGPMRLLAVAEISRGRLGTGAVFDEVIM
                     KVEPSAALETIDFQTEKPLHLGRAGQSSAFGHVDSMGKIFELPVVADFADGVTRRISP
                     PAGGTNYESSNQNIIKVLPGGLLQIVGNGKTTITVTNRGKQASLDVDVKVNDEPNESP
                     IADAGMNKSVKAGSRVKLSGLKSRDPEGEALYYSWSQVRGSKISLLDVNNSEASFLAP
                     TVSEPRMYRFRLRVTDKKGADSLPAFVDVTVEP"
     gene            1957..2154
                     /locus_tag="VN647_04165"
     CDS             1957..2154
                     /locus_tag="VN647_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWG96259.1"
                     /translation="MTTVTVSLSDEEMRRLEELSKREGLTVEQMVRLGINDFIGQPDD
                     SFRAAARRVMENNAELYRRLS"
     gene            complement(2466..3053)
                     /locus_tag="VN647_04170"
     CDS             complement(2466..3053)
                     /locus_tag="VN647_04170"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HWG96260.1"
                     /translation="MHSLKEMHRRGIAFAAVLGWSCCVTLVCSVAGAGAATIENSKSI
                     KVEKLLTLIRVSELESEYDSLTQRYVGEYEQQIRKSIASTYPNLAGEKKKEIERLLDV
                     FLKDIRKSIGQVETLHDHLKHAYARSFGEDELDRLIAHYSSPVGEKDAGMKKSAAIEY
                     NKMWTAQFMNQYKARVEKLFQVIAVVARGKAKQGA"
     gene            complement(3149..>3595)
                     /locus_tag="VN647_04175"
     CDS             complement(3149..>3595)
                     /locus_tag="VN647_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96261.1"
                     /translation="QIFYSPWRRSLRPLRGILHATFTFTMGALLFQRLSSWASGRDGT
                     ARWKKAGLTQRDLHRARFRCVEEVESVRYSLQDLHYADHHLGWLTGSGRRLVGLLTEA
                     IEQVERESERFKREVGRSAFGQPLRKHVQELQQARQTHGPVRLGKV"
BASE COUNT          865 a          980 c          894 g          856 t
ORIGIN      
        1 ctcctcaccc tatcgctcag tcctaagatc gttattggac tacgtcgaag aagtgctaga
       61 gaaggactca aacacctgga tcaccgtcgt gatcccggag atccttccgg caaggtggtg
      121 gcagaacatc ctacataatc agcgggcatt gatgctcaaa gcctccttgc tcttcaagga
      181 tcgggtgatt ctcatcgatg ttccttatca cctgacgagg tgaccgtgaa tcgtcatccg
      241 tcacccgtcg atcgtcacac acgtcttcgt gattctgtcg ttttcatttt tgccttctgt
      301 ctttgcttac cgctgcacgc actggcattc aagatcactg aaccggcagc tggaatgaaa
      361 ctttccgctg gaagcaccgt gaccacgcat gtggatcttg ggaaggacac cggtatcgtc
      421 aaagtccgtt actattggta ccgcgaacag gacgaggtgt tagtcgaaca ggatgactca
      481 acggccacag gctccatcgt ggcaccggta gcgatggtag gctcagcaga acaggaaccc
      541 gccttcggcg gctcactgaa agtgccggag gatggaatcg ggccgatgcg tctgttggcg
      601 gtggccgaaa tctcccgtgg acggttagga accggagctg tcttcgacga agtcatcatg
      661 aaggttgaac cctcagccgc cctcgaaact atcgattttc aaacggagaa gcctttgcac
      721 ctaggcagag cgggacagtc ctccgccttc ggccatgtgg actcgatggg taagattttc
      781 gaactcccgg tcgtcgccga ctttgccgac ggcgtcaccc gacgcatcag cccacccgcc
      841 ggcggcacca actatgaatc ttccaatcag aacatcatca aggtcttgcc cggcggctta
      901 cttcagattg tcggcaacgg gaaaaccacc atcactgtca ctaatcgtgg gaagcaggct
      961 tcactggatg tggatgtgaa ggtgaacgac gagccgaacg aatcgccgat cgccgacgcc
     1021 ggcatgaaca aatcggtcaa agccggctct cgggtgaaac tgagcgggct gaagagccgt
     1081 gacccggaag gcgaagccct ctactatagt tggagccaag tgcgcggcag caaaatttcg
     1141 ctgctggacg tcaacaactc agaggcatcc tttctcgcgc caaccgtctc ggagcctcga
     1201 atgtatcgct ttagattgcg tgtaacggac aaaaagggag ccgacagttt accggcgttt
     1261 gtggatgtga cggtggaacc gtaagtagtc ccctgaaaca gtctcctagg gagtccgcag
     1321 tcattgagaa tggagcgttc ggcctctggg atatatcacc tcaaaataag tttgcaggag
     1381 aagtattcct actgtatctc gtttcaagca ttctacaatc tgaacagttg caaaatggct
     1441 aggccatgac attcctcgat tcgttaaatg catgcaatca agaaggatat cgccctctta
     1501 ccacccagct cccccatact ccgaagacga tctattggag gcgttacata gagcatacgt
     1561 tgaggtccgc tatccagtgc cgcaaccatt ttataggacg aaggggcaag acgggaaaga
     1621 gcgtttctgc atttcgagcg acaaattcaa ggtatatcgc gacctcctta gcgagacaac
     1681 tccgcgtgac tacgctagaa gtatggctag gacattattg aaaaggatag aggatgagta
     1741 ttctgtaaag atctcctatt ctcaattctc gaacaagatt tcgtatcagg attgggaacg
     1801 attcacgaat gttttttatg ggagatgagc cttcaaaata caacccgcac aagaaaccaa
     1861 ctcagtacca gcttgcttcg tttgcccttc acagggcttt ccaatatact aaagccaatc
     1921 ataggttccc ccacgcgcta taaggagttg agtatcatga ccactgtaac agtatccctc
     1981 tccgatgaag aaatgcgccg actggaggag ctgagcaaac gggaggggct aactgttgaa
     2041 cagatggtcc gactcggtat caatgacttc attggccaac cagatgactc ctttcgtgct
     2101 gcagcgaggc gtgtcatgga gaataacgcc gagctctatc gtcgcctctc gtgatccgct
     2161 atctttccct accagaggtt ctcgaactcc accggtccgt gattgatcgc tggggtggtg
     2221 ctaccggaat tcgagatctc ggtgcattgg aatccgcttt ggcccaacca cgccagagtt
     2281 ttggatctag cagctgggaa tctttcgcga gatatatttg cggaatggat aaaacaacac
     2341 accgacccga ctaaaaaata gtcgctctcc cgactacccg tcgctgatca atgtgcttcg
     2401 ctgaactcgg gtagcgattc ttctcgcaga caggattcaa gtttacagtg ccgggctggt
     2461 tatcgctatg ctccctgctt ggccttgcct ctcgcaacta ctgcaatcac ctgaaaaagc
     2521 ttctcgactc gagccttgta ttggttcatg aattgagccg tccacatctt attatattca
     2581 attgcagcac tcttcttcat tcccgcatct ttttcaccaa ccggagagga atagtgggca
     2641 atgagacggt caagctcgtc ctcaccaaaa ctcctcgcat aggcatgctt taaatggtca
     2701 tggagcgttt caacctgtcc gatcgatttt ctgatgtcct tcaagaatac gtcgagaagc
     2761 cgttcgattt ccttcttttt ttcaccagcc agattagggt aggtgctcgc tatggatttt
     2821 cgtatttgtt gctcgtattc accgacatat ctttgagtga gtgagtcata ttccgactcc
     2881 agctccgaga cacggataag agtcagcagt ttctcaacct tgatcgactt tgagttctcg
     2941 atcgttgccg ctcccgcccc agctacactg caaaccagcg tcacgcaaca ggaccaacct
     3001 aatacagcag caaacgcgat tcctctacga tgcatctctt tcaagctgtg catggctctc
     3061 acctcttgta atcgagtcta catcgtatgg accgatcgtc aagaaatata aaatcttcca
     3121 tcgtcagact ttgctctacc agacaacgct acacctttcc taaccgaacc ggtccatggg
     3181 tttgacgggc ttgctggagt tcttgaacat gcttccgcag aggctggccg aaggcggaac
     3241 gtccgacttc tcgtttaaat cgctcactct cacgttcgac ctgttcgatc gcttcggtca
     3301 gcagtccgac caagcgccgg cctgaaccgg tgagccaccc gagatggtga tcggcatagt
     3361 gcaggtcttg cagcgagtag cggacggact cgacttcttc gacacaacga aagcgcgctc
     3421 ggtgcaagtc tcgctgtgta agaccggcct tcttccatct tgccgtcccg tctcgtcccg
     3481 acgcccagga cgaaagccgc tgaaataaga gcgcacccat ggtgaacgtg aaggtcgcgt
     3541 gaagaatgcc gcgcaacggc cggagactcc gccgccaggg tgagtagaag atctg
//
LOCUS       DATNDD010000175         4801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2942176, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000175 DATNDD010000000
VERSION     DATNDD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942176"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VN647_04180"
     CDS             <1..511
                     /locus_tag="VN647_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWG96262.1"
                     /translation="FALCLMVGCTAVPRKYLQEADSTLRFSWLAATPERYQGRLVILG
                     AVIVKEEVRAGDLWLHVKNRPLNEKYRPQLPPSPSDQEGGWYWLVVKKHHTLPSSYHH
                     WADMTIVGRVIGVGPEKQPLLTLIYARGWGMNREHDGVWEHLTDKNYGVMTPPELIRD
                     SAPLQNIEQ"
     gene            2903..3766
                     /locus_tag="VN647_04185"
     CDS             2903..3766
                     /locus_tag="VN647_04185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG96263.1"
                     /translation="MSIGDNRAVVITGASTGIGAACALHLDRIGFAVFAGVRKSEDGM
                     ALQKSGSDRLVPVELDVTDLTTIQKSHAVVLEATRERGLFGLINNAGIAVVGPLEAVP
                     ISDLRQQLEVNVIGQVAVIQAFLPLVRQARGRIVNMGSIAGLSTMPLMGPYSASKFAL
                     EAITDALRLEVQQWRIHVAIVEPGAIATPIWNKSAIEAAEREAAIETELRTLYKSVVA
                     SVRKVVGEASKRAISADAVAKVVEDALTASTPKTRYLVGADAKLRALMVRLLPDRISD
                     RMLTWILKLPH"
     gene            complement(3835..4239)
                     /locus_tag="VN647_04190"
     CDS             complement(3835..4239)
                     /locus_tag="VN647_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96264.1"
                     /translation="MWAIVFLLLSLCPPLLTPLGSTGPAAAVSLIEIAELLAHPELYD
                     HQDVVVTGKVMNVQLATNRQGQPAYGFLLQDHAGTLKVVSLGQVEVHEGDQVIVEGVF
                     SRLRQVGRTIVYNEIKALSVKPLTRLNPDFVG"
     gene            complement(4327..>4801)
                     /locus_tag="VN647_04195"
     CDS             complement(4327..>4801)
                     /locus_tag="VN647_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96265.1"
                     /translation="VAAGSGNSLSLDLWKETMQVLSRTPVERSIATYDGLERTESALL
                     EKLTAYALIRIPEVVETKFWNSLVYGASSNAVHSETTRTLFGLQRLVSVQKDLDAGDE
                     ALDEELLSRTTPISKEAEPVANQNAAGQSELTELQQHILRSFIGDDSSGRASDKW"
BASE COUNT         1186 a         1180 c         1290 g         1145 t
ORIGIN      
        1 attcgcacta tgcctgatgg tggggtgcac tgccgtgccg cgaaaatatc tgcaggaagc
       61 agactcaacg ctcagattct catggttggc cgccacgcct gagcgttatc aagggcgcct
      121 agtcatctta ggggctgtca tcgtgaagga ggaagtccgg gctggagacc tctggttgca
      181 cgtcaagaat cgcccgctca atgagaagta ccgtccccaa cttccgccca gccccagcga
      241 tcaagagggc gggtggtatt ggctcgtcgt gaagaaacat cacacgttgc ccagttccta
      301 tcatcattgg gctgatatga cgatcgtcgg tcgtgttatc ggtgttggcc cagaaaaaca
      361 gcccctcttg acgttgatct atgcacgcgg gtggggaatg aatcgggaac atgacggagt
      421 ctgggaacat cttaccgata agaattatgg ggtcatgact ccccctgagc tcattcgtga
      481 ttcggctcca cttcagaaca ttgagcagta gtctgatacc aaaattgcta ccgctgtgga
      541 cggtaagacg atcaaagcgc tggtattgga agcactagaa gtaaagattc aggagctgga
      601 aaagaagggg ctgctgccga aggggaagta atgagataag gtacgccagc aatggccaaa
      661 tctgtaacct acaaggacta caccattcaa cccgcccctc ggcagctggt agataccggc
      721 cagtgggaat tgaatgtctt catttcgtgg tcaacggagg acgacgagga tagtcgtcat
      781 ttcgtgaaga ctggcaggta tgcgacagag gaagaggcca cggctcagtg cattatctat
      841 ggtcaacaga tcatagacgg taatgttcca ggatcatcag tgagataaag cgcgttccac
      901 atcccggaag cgaagccggt tcgctgtagc cgctgagttt tgaggcagcg agagatatga
      961 attgcctcat gatgagctaa gtttgtcgat acaaatcact ttaaatggag agctacctac
     1021 aagagtgaac gtgttcgagt tagtttttgt aatattctgt atcaatttga atacttgata
     1081 gtatccgctg cgaaagtgtc cgttatcgct tgtacgtatt atatttcgag acgaatcatc
     1141 cagccgaaac tcataatccg cattgatacg gatggcaacg gtctgagaaa tattgaagtt
     1201 aggccatacc tctacctgct tggcatacgc cttggtaccg tcatatggca atggtgtagg
     1261 gagctgcttc tctcgaaagc gcaacctttc attaatacac aattttgaaa tgagtgcgtt
     1321 tcttatctcg ttgtcctgct gcacgccgtg caatggcttg gggatacgct ttgatgaaga
     1381 actcttcacg atctcatcat acaaacttca ttttggaggc gtactcttaa aaaagaaata
     1441 gaaaggggat gtcatcatgc ctatcttgat cgaagtatta atcttggccc caatattcgg
     1501 actgctgtac ggaatcttgc ggtttgccag tcggtaacga gttcgactct cgcccagggt
     1561 cgctcctttt tcgagcaccg tctggccgtg gggttttatc cttggccttt acactgacca
     1621 atgatacccg atcgccactg cccctggtgt aaggttccca tgagaaaacg gctggtcgat
     1681 ggcgaactcc ttatccacta tacttgtccg aagtgcattt tccagcacac gcgcccccta
     1741 cgcctccaaa tcgaaggtga caccccaccg gtatagcgtc cagtaaccaa caagcaataa
     1801 aaaacccctg aagccgtgcg actccagggg attcgatttc tcgataggct aaataactgc
     1861 tggatgccta gctagcagag cagtttcatg ttacgccgtc agaatatagc ggttttgggc
     1921 ttacgcgtgg taacgctggc atatcattca catggccttt gtgaaagtgt ctcgctcaag
     1981 aaatgtggaa ggagaggagc gcgacttgga ggaggagatt tcgtcagact cacgatccac
     2041 tgttggcatt ttggcaaggc ctaatctcca gccgcttcct tgacacccct ttccacatcg
     2101 gcatagcatt agttgccata tacgttcctg cagaagaaga tcttaattag acatcctgta
     2161 tggaatctga aagagggaat gcacggccat gaaaacgctt atcataattg ctcgggattc
     2221 catgctcaag gagttggagg agcttttgcg taataatggt gtcatggcat ataccaccct
     2281 tagcaacgta tcaggtcaag gtgtaaccgg taaagtgtat gggaccttcc ttaaccccgc
     2341 tattaatgcc gtcatttgtg cagttcttac aacagacgaa gcagacaagg caatcatagc
     2401 ccttaagacg tttcatacaa ggcgaaagga gactgcgccc cgggatcaac ctattccact
     2461 taaagtattc tcgtggtttt gtgacgagca cgtatagcga gattgattta caactaatac
     2521 aacatgcttt tgtacctgcc atccagagtg acttgcggaa ggatattgtc gaatggatga
     2581 aaccactgcc tccactgtgc ccgtaattgt ggccgcgagc ggctatatag caccattctc
     2641 caccccccca gcaaccaatg aggcaataaa aaaccccttg taaccgttga agattacaag
     2701 gggctcttgt tatctggttg gcttatgagt ccgctgctct gccaactgag ctacaccgcc
     2761 acaacgtcac tcagaagaaa ccaaatgata actgaagatc gaggccggtg tccaccgagt
     2821 gagttggaaa tgctgcgcca tgtatggatg aagtcgcgtg aaacctggta tggtcctccg
     2881 gtcgcgattc aaaaggagca agatgtcgat cggggacaat cgcgcggtgg tcatcacggg
     2941 agcgtctact ggaattgggg cggcctgcgc gctgcatctt gatcgaatag gattcgccgt
     3001 ttttgcagga gtgcgaaaat cggaagacgg catggctctt caaaaatccg gttccgatcg
     3061 gctggtcccg gtggaactcg atgtgacgga cctcacaacc attcagaaat cccacgcggt
     3121 cgtgttagaa gcgacgagag agcgcggatt atttgggctc attaataatg ctggaattgc
     3181 ggtggtgggg ccgcttgaag ccgtaccgat ctccgacctc cgccagcagc tggaggtcaa
     3241 cgtcatcgga caggtggcag tcattcaggc atttttgccc ttggtccggc aggcacgggg
     3301 acggatcgtc aacatgggtt ccatcgcagg actctcgacc atgccgctta tgggccccta
     3361 ttcggcatcg aagttcgcac tggaagccat tacagacgcc ttgcgcctcg aagtccaaca
     3421 atggaggatt catgtcgcga ttgtcgagcc gggtgcgatc gccacgccta tctggaacaa
     3481 gtcggctata gaggctgccg agagagaagc agccatagag acggaacttc gaactctcta
     3541 taagtctgtt gtggcctctg tgaggaaggt cgtcggggaa gcatcgaaac gagcgatctc
     3601 agctgatgcg gtagcaaaag tcgttgaaga tgcgttgacc gcgtcgactc ccaagacgcg
     3661 atatctcgtt ggagccgatg caaagttgcg cgcccttatg gtgaggcttc ttcccgatcg
     3721 gatatcagat cgaatgctca cctggatttt aaagcttccg cactgagtga ccgagcaacc
     3781 cttcaaaaac taagctaaga ccggaaggat gacttgctga tgaggagcga cgaatcagcc
     3841 gacgaagtcc gggttcaggc gagtcagcgg ttttactgaa agggccttga tctcattgta
     3901 gacgatcgta cggccgactt gtctgaggcg actgaagaca ccctcgacaa tcacttgatc
     3961 accttcgtga acttccacct gcccaagact cacgactttg agcgtgccgg catggtcttg
     4021 gagtaagaat ccataagccg gttgaccctg acgattggtc gccaactgta cgttcatgac
     4081 cttccctgtg acgacgacat cttgatggtc gtacagctca ggatgtgcca gaagttctgc
     4141 gatctcaatc aggctgacgg cagcagcagg accggtggag ccgagcggtg taagcaatgg
     4201 tgggcagaga gacagaagga ggaagacgat tgcccacata agaaaaggtc gttgggtggc
     4261 gagctgcgtc gtcatcgttg gtggtgcaat tataccgaac tgccggtgat tggcaagggt
     4321 gaaagtttac catttgtcgg aagctctgcc gctgctgtca tctccgataa aactacgaag
     4381 aatgtgttgt tgcagctccg tcagctcgga ttggccggcg gcattttgat ttgcgactgg
     4441 ctccgcttct ttcgatatcg gcgtcgtgcg gctgaggagt tcttcatcca gcgcctcgtc
     4501 gccggcgtcc aaatcttttt gtacggagac cagccgttgg agtccaaata atgtccgcgt
     4561 cgtttcactg tgcacggcgt tggaactggc accgtacacg agcgaattcc aaaatttcgt
     4621 ttcaacgacc tccggtatgc gaatcagggc ataggccgtc agtttttcaa gcaaagccga
     4681 ctcagtccgc tcgagaccat cgtaggtggc tatcgatcgt tcgacaggag tacgagagag
     4741 aacctgcatg gtttctttcc agagatcaag ggagagactg ttgcccgatc ctgcggcaac
     4801 g
//
LOCUS       DATNDD010000176         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1402591, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000176 DATNDD010000000
VERSION     DATNDD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402591"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VN647_04200"
     CDS             <1..255
                     /locus_tag="VN647_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96266.1"
                     /translation="KSAGRIALELKDKVGKIHGIRPHATAAAAPELDGPYEDALSALV
                     NLGYRAQDAKEALKRVIKAAPGSLGLKELIRNGLKELARG"
     gene            386..748
                     /locus_tag="VN647_04205"
     CDS             386..748
                     /locus_tag="VN647_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96267.1"
                     /translation="MTCGTCPEGYATTGVTQSPEICKDEDPTLVQCVPLGANMLGVCG
                     TCPDGYVEIGSSSVPARCGSKDSGRMTQCQLRKMEQNFPDSTQGYKRCPPDCGSTAQP
                     GQGTLPPPPKYQQMPESK"
     gene            764..1768
                     /gene="ruvB"
                     /locus_tag="VN647_04210"
     CDS             764..1768
                     /gene="ruvB"
                     /locus_tag="VN647_04210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWG96268.1"
                     /translation="MTERFLTNRATDEERGQENVLRPQALTEYVGQDKMKESLRICIE
                     AAKQREEALDHAIFYGPPGLGKTTIAHIIAREMGATLRSTSGLVLAHAGDLAAILTNL
                     QEHDVLFIDEIHRLPASVEEALYPAMEDFQLDLVIGQGPAARIVKLDLPPFTLVGATT
                     RAGSLTSPLRDRFGLVYRLEFYGPSELEAIVTRSAGVLGIGIDRAGAEEISRRARGTP
                     RIVNRLIRRIRDYAQIKADGHITEQVAKEGLAWVGIDEAGFDDMDRKILITIIEKFNG
                     GPVGVESLAAAVQEDKGTIEDVYEPYLIQAGFLDRTGRGRQVTRLAYDHFKRPSPLLM
                     "
     gene            1930..>2447
                     /gene="pheA"
                     /locus_tag="VN647_04215"
     CDS             1930..>2447
                     /gene="pheA"
                     /locus_tag="VN647_04215"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWG96269.1"
                     /translation="MSKDMAEYRKEIDRIDDQIIRLLNERSKSVIEIGKIKKEKNADA
                     NLHTAGREAEIIERLTKLSTGPFPSEAIRSVYREIMSASLSLESPQKVAYLGPRATFT
                     HMACMQKFGSSVQYVPVHSIKEVFSEVERSRANFGVVPIENTTEGVVNHTLDMFIDSN
                     LLIYGEILQEVS"
BASE COUNT          596 a          639 c          693 g          519 t
ORIGIN      
        1 aaatcggcgg gtcgtattgc acttgaactg aaagacaagg tgggcaaaat ccatggtatt
       61 cgcccccacg ccactgccgc tgccgcgcct gagctggatg gtccttacga ggatgcgctt
      121 tccgctctgg taaacttggg ctatcgtgct caggatgcca aggaagcctt aaagcgggtg
      181 ataaaggctg cgcccggatc gttggggctg aaagagctca tccgcaacgg gctcaaggaa
      241 cttgccaggg ggtaagccta tgccgctcaa cgtcaggaac atcggtcaga tgctcgtgta
      301 cagtatctct atcggggcgg ttttcgtagt cgcattccca actcggagtc tcgcgcaaga
      361 ggccacggag ccgaaaagca tcagaatgac ctgcggaacg tgtccggagg ggtatgcaac
      421 gaccggcgtc acccaatctc ccgaaatctg caaagacgaa gatccgactc tcgtgcaatg
      481 tgtgccgctg ggggcaaaca tgctgggcgt gtgcgggacg tgtcccgacg gctatgtcga
      541 aattggaagt tcctcagtgc ctgctcgatg cgggagtaaa gacagtggtc ggatgacgca
      601 atgtcaattg aggaaaatgg agcaaaattt tcctgactcg acgcagggat ataagcgatg
      661 tcctcctgat tgcggcagta cggcccagcc tggtcaagga actttgccgc ctcccccgaa
      721 gtatcaacag atgccggaga gcaagtagaa tgggcgtcgc accatgaccg aacggttcct
      781 caccaatcgc gccacggacg aagagcgtgg ccaagagaac gtccttcgac ctcaggcgct
      841 cactgagtac gtcggtcaag ataaaatgaa ggaatcgctt cgaatctgca tcgaagcggc
      901 taaacagcga gaagaagcgc tcgaccatgc gatcttctac ggaccgcccg gcctcggaaa
      961 gacgaccatc gcccacatca ttgcgaggga aatgggggcg acattgcggt cgacgtcggg
     1021 actggtccta gcccatgccg gcgacttggc ggcgattctg accaatcttc aggaacatga
     1081 tgtgctcttt attgatgaga ttcaccgtct gcccgcgtcc gttgaggaag cactgtatcc
     1141 ggccatggag gattttcaac tggacctggt gattggacag gggcctgccg cgagaatcgt
     1201 taagctggat cttccgccat tcacactggt aggggcgacg accagggccg gctctctaac
     1261 atcccctctg cgagacagat tcggcttagt ctatcggttg gagttctatg ggccgtccga
     1321 attggaagcg attgtgactc gctcggccgg ggtcttaggt attgggattg accgggcggg
     1381 tgccgaggaa atctcacgcc gagcgcgtgg aacaccgcgg atagtgaacc ggctgattag
     1441 aaggatcagg gactatgcac aaatcaaggc tgatgggcat atcaccgaac aggtggccaa
     1501 agagggatta gcctgggtgg ggatcgatga ggcaggcttc gacgatatgg atcgcaaaat
     1561 ccttatcacc attatcgaga agttcaatgg tgggcctgtc ggggtggagt ccttggctgc
     1621 cgcggtccag gaagacaagg gcacaatcga ggatgtgtat gagccctatc tgatccaagc
     1681 cggtttcctg gatcgtaccg gtcgtggtcg gcaagtgact cgtttggcgt acgatcactt
     1741 taaacggcct tctcccttac tgatgtaaga cccctgtaca ttccaaccag aaacttcctg
     1801 atatccaagt gctatccttg caatagattc aatcgttcct gtaaaatcca ctacttgtcc
     1861 ctatgatccc cttgccctcc ggggatgagg aagcccttcc cgcaatcggg tgccgagacg
     1921 agactcagca tgtccaaaga tatggcagaa tatcgtaagg aaatcgatag gatcgatgac
     1981 caaattatcc gattgctcaa tgagcgttct aaaagcgtca tcgaaatcgg aaaaatcaaa
     2041 aaagaaaaga atgctgatgc caacctccat accgcaggtc gggaggctga gatcatcgaa
     2101 cggcttacga aactcagcac gggccctttc cccagcgagg ctatccgatc agtctaccga
     2161 gaaatcatgt cggcttcctt gtcgctcgag tctccgcaga aggtagcgta tctgggaccc
     2221 cgagccacct ttactcacat ggcttgcatg cagaaattcg gctcgtccgt ccaatacgtg
     2281 cctgttcaca gcatcaaaga ggtattcagc gaagttgaac gaagccgagc caatttcggg
     2341 gtggtaccga ttgaaaacac caccgagggt gtcgtgaatc acactctcga catgtttatc
     2401 gattccaact tgctgatcta tggagagatt cttcaagagg tctcaca
//
LOCUS       DATNDD010000177         3294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1851150, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000177 DATNDD010000000
VERSION     DATNDD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851150"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VN647_04220"
     CDS             <1..372
                     /locus_tag="VN647_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HWG96270.1"
                     /translation="VPLAGYYYCPHDPEGDVPLFNVDCYCRKPNPGLLIQAANELHVN
                     LTQSWMIGDILHDVEAGRAAGCRTILLTNGNETEWHLTPLRWPNLIADEVFEAARLIV
                     FTDLIATEERHCFQDDGLKES"
     gene            complement(491..946)
                     /locus_tag="VN647_04225"
     CDS             complement(491..946)
                     /locus_tag="VN647_04225"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWG96271.1"
                     /translation="MQSASALQDTMSEYTRHCVRSTERYALERHQFRSEGTVFAKNKD
                     GKLVICPATNGTEPYTLQRRGAVVAVRVSPVAPFTVVNMIAGATHIRTRDFLSRTVLG
                     ELPGLSAIAVFVDQVTTILHSPNLGSYLVLAGSVIVVWGLRCRLSRRAL"
     gene            1597..2205
                     /locus_tag="VN647_04230"
     CDS             1597..2205
                     /locus_tag="VN647_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWG96272.1"
                     /translation="MPKPDSGKAKAKSPSQKKRSTDAIQMLKDDHRKVEVLFDQFLEE
                     ESGKKQQLAQQIFHELEVHSTLEEELFYPALQNQGDPAELGSLEQDEEIEGKEAMDAS
                     ELDEGEEMERDGDEDETAEDMIAGAYDDHRVVRDMIAQLRNNDPSSLEFRQSMIELQQ
                     TVAGHVSQEEDELFPQAQLSMDTKTLGMQMQQRKQEILSAAA"
     gene            complement(2264..>3294)
                     /locus_tag="VN647_04235"
     CDS             complement(2264..>3294)
                     /locus_tag="VN647_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96273.1"
                     /translation="VARVAKEPGTPLLAELDQIFDESLRYTRTLIADLAPPSLQEFGL
                     SHAFQWLSEHMRTHGLTVDVQVGSDRINLPDDQAILVFQSVRELLLNIVKHAETNRAS
                     VTVATSPTELSITVTDGGQGFDHAVVGAPEPGSSRFGLFSIDERMRAMGGRFEINSTP
                     GRGTRASMILPYWPASAAPELWEVGKADQRSSLTTQTSPEQPMVRILLVDDHVMVRRG
                     IKSLLDGHENLRVIGEASDGQQAVKLTAELRPDVVLMDVNLPVMDGVTATSRIHRDYP
                     DTVTVALSVHDDRQIIDAMTKAGAAAFVSKGSVTEELYEAISRALLSKGKLPGSPRRD
                     KLSLSSKV"
BASE COUNT          786 a          936 c          921 g          651 t
ORIGIN      
        1 gtccctcttg caggctatta ctactgtcct catgatccag agggcgacgt ccccttgttc
       61 aacgtggact gctattgccg caaacccaat cccggtctgc tgatccaagc cgccaatgaa
      121 ctccatgtga atctgaccca gtcatggatg atcggcgata tcctgcacga tgttgaagcc
      181 ggccgagcag ccggatgccg caccatctta ctcacgaacg gcaatgaaac cgaatggcat
      241 ctcacgcctt tgcgttggcc taacctcatt gccgacgagg tattcgaagc cgcccggctc
      301 atcgtgttca ccgacttgat tgcgaccgaa gaacgacact gcttccaaga tgacggattg
      361 aaggagtcct gatggcgatc gtcattgatc gaccgagagc cccgcctctg cgccttcgga
      421 tgaggcttct caccccagca gaacgttcat gcggcgcgtg aggttgtctc gcgtcgacga
      481 tgcttatcgt tcataaagcg cgtcgagaga gccggcatcg caacccccac acgacgatga
      541 ccgacccggc caggacgaga taactcccaa gattcggact gtgcaatata gtcgtcactt
      601 ggtccacgaa aacggcgatg gccgatagac cgggcaattc gcccaggaca gtcctcgaca
      661 gaaaatccct cgttcgaata tgtgtggcac ccgcaatcat attgaccacc gtgaatggag
      721 cgaccggact cacccgcacg gcaacgacag ccccacgcct ttggagggta tatggctctg
      781 ttccgttcgt cgctgggcat atcactagct ttccatcctt atttttcgca aagacggtgc
      841 cctcggaacg aaattgatgt ctctccaatg cgtaccgttc cgttgaccgg acacaatgtc
      901 ttgtatactc agacatcgtg tcttgtagag cagacgcgga ctgcattatt tcaatgatca
      961 atcaaccctc acaaaggggt ctgtattttg caccgactgc ttgcttgaat gtttgcccac
     1021 atactttttt atcgaaggag gatttttatg agcagaggac gggtgtgcgc gtacatgttg
     1081 atggtcggag gaatgctcct cttcgccagt ggcgcatctc aggcggaaca atcaaagagt
     1141 ccaaagagtg gaacccaatc cggtaacaca accatgggga ccggatccgg aagtaccggt
     1201 ggatcaagcg gaaccatgaa gcaaggccac gaatcgtcgg gcaagttggg agcagaacca
     1261 cacggtcaat ccagcggacc aaaaactggt ggtgaggcaa ctctgggagg atccggacaa
     1321 atccctgact cgagcaaggt tcctggtaag gaatcaagcg gcggaagcgg atccagttca
     1381 gtaccaggca ccggtagctc cggaaacatg ggatcatcgg gcggaacggg ctcatccggc
     1441 ggcacagggt cctcgggtgg agccggcggc tattaatctt cttattttgt catatctcac
     1501 acagccgact gaggcaggga gggaaaggtt cctacctgcc tcatggcatg gcacacacta
     1561 tgccgacgcc caggtatcat cagaaaggag acacctatgc cgaagcccga ttcaggcaaa
     1621 gccaaggcga aatcgccgtc ccaaaagaag cgatccaccg acgcgataca aatgctgaaa
     1681 gacgatcacc gtaaagtcga ggtacttttc gatcaattcc tggaagaaga aagcgggaag
     1741 aaacagcaac tcgcacaaca gatttttcat gagctggagg tgcacagcac cttggaagaa
     1801 gagctctttt atccggcgct tcagaatcaa ggcgaccccg cagaactcgg ttcgctcgaa
     1861 caggatgagg agatcgaagg aaaagaggcg atggatgcat cggagctcga cgaaggggag
     1921 gaaatggaac gggatggaga cgaggacgag accgccgaag atatgatcgc cggcgcatac
     1981 gacgatcatc gtgtcgttcg agacatgatc gctcagctca ggaacaatga tccatcaagt
     2041 ctggagttcc gacagagcat gatcgaattg caacagacgg tggcgggtca tgtgtcccag
     2101 gaagaagacg agctcttccc ccaagctcag ctcagcatgg ataccaagac gctcggcatg
     2161 caaatgcagc aacggaagca ggagattctt tccgccgccg cctgaatcgg ctcggcacat
     2221 gggccacggg ggtgacaccc cttccctggc tccgcgcggc gggctatacc ttgctggaca
     2281 gagacagctt gtctcttcga ggagagccgg gcaatttgcc tttggaaagc aatgcgcggc
     2341 tgatcgcctc gtacaactct tccgtcacgg accctttcga cacgaaggcc gccgcaccgg
     2401 ccttcgtcat cgcatcgatg atctggcgat cgtcatggac agacaacgcg acggtgacgg
     2461 tatcgggata gtcccggtgg atccggcttg tcgccgtcac cccgtccatc acgggaagat
     2521 tcacgtccat gagcacgaca tcgggccgca gctccgccgt caacttgacg gcctgttgtc
     2581 catcggaggc ctctccgatg acccgcaagt tctcatgtcc gtccagcagg cttttgatcc
     2641 cgcgccggac catgacatgg tcatcgacaa ggagtatgcg gaccatcggc tgttccggcg
     2701 aggtctgcgt ggtgagactc gagcgttggt ccgctttgcc gacttcccac agctccggtg
     2761 cggcggaggc aggccaatag ggcagaatca tggaggcccg tgttccacga ccgggcgtgg
     2821 aattgatctc aaaccgtcct cccatcgccc gcattcgctc atcgatgctg aacagcccga
     2881 atctggatga cccaggttcg ggagcaccta caaccgcatg atcgaacccc tgtccgccgt
     2941 ccgtgacggt aatggacagt tcagtgggac tagtggccac cgtcacactc gcgcgattcg
     3001 tctccgcatg cttcacgatg ttgagcagca gttctcgaac ggactgaaaa accaaaatcg
     3061 cttgatcgtc cgggagattg attcggtccg acccgacctg cacgtccacg gtcaatccat
     3121 gggtacgcat gtgttcggac aaccattgaa aggcatgaga caacccgaat tcttgcagtg
     3181 aaggaggagc cagatcggca atgagggtgc gtgtataccg caaggattcg tcgaaaatct
     3241 gatctaattc ggcgagcagc ggcgttccgg gctccttggc gacacgggcc acgg
//
LOCUS       DATNDD010000178         4176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_449156, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000178 DATNDD010000000
VERSION     DATNDD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449156"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VN647_04240"
     CDS             complement(<1..548)
                     /locus_tag="VN647_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96274.1"
                     /translation="MRARILIVDDDPDIALPLERRLIGMGHETLIVNNGKDGLRLIHQ
                     EQPDLVLLDLELPFVSGFEVLRQVRDRCHPAEEGAPSVRVTYTTPLIVIFTAYGTIER
                     AVQAMQLGAFDFIPKPFTKDHLAVVINKALDTVALHRQVETLRKEVDAQFEPIVSTSK
                     RMSDQLTVAKQAAASSATVLLLG"
     gene            complement(556..2796)
                     /locus_tag="VN647_04245"
     CDS             complement(556..2796)
                     /locus_tag="VN647_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWG96275.1"
                     /translation="MADTDHLLVPGEFEFLPKTRRILERPLMLFRPFGKDVDGVAIRD
                     MTGVSIRSNVEHLEEYVTQTQGTQTGEEVVETLVKLLNERIPDRSYHVTSRFLRNPWT
                     CYSNEFTAYLVEFCIELSRDNGFQFNMGRKKLIPPIMQTLMRPFSVKSIYKGATRWIE
                     HYAKDSYDLQGIEVRDGYAILRMTLMDRALKQFGPYRRACAKIWCNALKVGISIVPQK
                     IHGLEPAAMFERKCIVEGDDFCEWQVRWKEPVRWYLGKQIATNIAHHVLRHEIVDRDR
                     LIEEQTFSLKTRHEELEKAYVELQQTAVELRKRVDYLTTLYEAGLQFTSSRDSEVLLQ
                     HALEILINQLSYDRVMISFFDSTRKISHSSRVVGVTDALAAFARNLEVPVTDPLTIEG
                     AVLLHGCPVLVQNVHEVLDRLHPLHRELAFKSGTQSFVSVPLKTKNGILGSLTVDRSQ
                     ANSLNQDDLEIMTTFANQLAIAVDNVTAYSQIEQLNLGLEQKVLERTAELEAANVQLK
                     ELNQLKSAFVSVVSHELRTPMTSIRVYVENMLDGLIGSLTDEQSRYLNRILFNVDRLT
                     RLSTDLLDLSKIESGKVELRLERLSFDDVLSDIVEGFRHSLKENSIALDVSSADKAVT
                     IHADRDKLAQIFTNLFSNAIKFTPAGGKIQILTKVQPDGYLHIRISDTGCGIAPEELS
                     KVFEKFFRGESTPSGIRGAGLGLAIVKNLVELHGGTVGVESTVGQGSTFFFTVPTSQT
                     SVLTPS"
     gene            complement(2883..>4176)
                     /locus_tag="VN647_04250"
     CDS             complement(2883..>4176)
                     /locus_tag="VN647_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWG96276.1"
                     /translation="PFPSHSEAFRILDQGAAASGQGTAFAAQADDPSAIHYNPAGMTQ
                     LSGMQFYGGTLLVGGHYDYTAPTGARYRANLDGAIAFPPPINFYLTANFGKLNIDFLK
                     NFSIGLGINSPYGLIINYPREVPFSALDTRATLPLIDIKPTFAYRFNDYLSIGGGLDI
                     YTFTSFAGAGGVQINAFVPPSTNLELNAKDTAIGYNIGVLLTPWRSDEKPRLNLAFVY
                     RSQATLNFHGDFLMNGGLLANARVDLTLPEIVTGAVAYWPIRDGNREWKLEVDVDYAN
                     WHTFKQVDIQLSNGAILPEPRNWKGVFIIHTGTEYKWIGSSILPEWDISARFGYIRSQ
                     TPVPENTFEPAVPDANYNSFSVGCGFLCKGQGKFFGFIKCQNHLVGLTSIKSIGLDLA
                     YKNQIYESRTITNDIRPIVNGTWETILHAGVMSLRINF"
BASE COUNT         1016 a          988 c         1088 g         1084 t
ORIGIN      
        1 ccgagcaaca agacggtcgc ggaggatgcc gcggcctgtt tggccaccgt cagttggtcg
       61 ctcatccgct tactggtgga gacgatcggc tcgaactggg catcgacttc cttgcgaagg
      121 gtctcgacct gacggtgaag ggcgacagta tctagagcct tgttgatgac cacggctaag
      181 tgatctttag tgaacggctt cggtataaag tcgaaagcgc ccagttgcat ggcttgcacc
      241 gctcgctcga tcgttccata tgccgtgaag ataacgataa gtggtgttgt ataagttact
      301 cgaactgagg gagcgccctc ttctgctggg tggcatctat cgcgaacctg ccgtaacact
      361 tcaaagccgg acacgaatgg gagttccaaa tctagaagca cgagatccgg ctgttcttgg
      421 tggatcaggc gaagcccgtc cttgccgtta ttcacgatca atgtctcatg gcccatcccg
      481 atcaaacgtc tttctagagg caaggcgata tcggggtcat catcaacgat gagtatgcgt
      541 gctctcatgg gacctctatg aaggagttag aaccgacgtc tggctcgtcg gaaccgtaaa
      601 gaagaaagta ctgccctggc cgactgtgct ttccactccg accgtacctc catggagttc
      661 aaccagattt tttacaatgg ccaagccgag tcctgctcct cggattcctg acggtgtcga
      721 ctctccgcgg aagaatttct caaaaacttt ggagagctcc tctggtgcga taccgcagcc
      781 ggtatccgag atgcggatgt ggaggtaccc atcgggctgc accttggtca gaatctgaat
      841 cttgccgccg gcaggggtga actttatggc gttgctgaag aggttcgtaa agatctgtgc
      901 aagtttatcc cggtcggcgt ggattgtcac cgctttatcg gcagatgaaa cgtcgagcgc
      961 gatagaattc tctttcaatg aatggcgaaa gccttcaacg atgtcgctga gtacgtcatc
     1021 gaaggatagc cgttcgagcc ggagctcaac cttgcccgat tctattttgg agagatctag
     1081 aaggtcggtg ctcagtctcg tcagtcggtc aacgttgaag agtattcggt taaggtagcg
     1141 ggactgttca tcggtcagcg agccgattag gccatccaac atgttttcga catatacacg
     1201 gatcgatgtc ataggcgtgc gcagttcatg ggagacgacg gaaacgaacg ctgatttgag
     1261 ctgattcaat tccttcaact gcacattggc agcttcgagt tctgcggtac gctcaaggac
     1321 cttttgctca aggcccaagt tcaactgctc aatttggctg tatgctgtta cgttatcgac
     1381 agcaatggcg agttgattgg caaacgtagt cataatttct aaatcgtctt ggttcaagct
     1441 attagcttgg ctacgatcta cggtcagaga gcctagaatt ccgttctttg tcttaagcgg
     1501 tacagataca aatgattgag tgccactctt gaatgccagt tctctatgga gaggatggag
     1561 ccggtccagt acctcgtgaa cattctgaac taagactggg caaccatgaa ggagaacggc
     1621 gccctcgatc gtcaatgggt cggttactgg aacctccaga tttcgagcga acgcagcaag
     1681 tgcatcagta actcctacaa ccctgctgct atgtgatatc ttgcgggtgg agtcgaagaa
     1741 gctgatcatg acacggtcat atgaaagttg gttaattagg atctctaggg catgttgaag
     1801 aagaacttca gagtctctcg aagaggtaaa ttgcaggccg gcttcgtaga gagtggttaa
     1861 ataatcgact cttttcctta actccacagc agtctgctgc aactcaacgt aagccttctc
     1921 aagctcctcg tggcgagtct tgagcgagaa ggtttgttcc tcgatcagtc gatcgcgatc
     1981 gacgatttcg tgtcgtaata cgtggtgcgc tatgttggtt gctatttgct ttccaaggta
     2041 ccaacgtact ggttctttcc agcgaacctg ccattcgcag aagtcatctc cctccactat
     2101 acacttgcgt tcgaacatag ctgccggttc aaggccatgg atcttttgtg gaacgatgga
     2161 tatccccact ttgagggcgt tacaccagat tttcgcgcag gcacgacgat atggtccaaa
     2221 ttgttttaag gctcgatcca tgagagtcat gcgaaggatg gcgtaaccgt ctctgacttc
     2281 tattccttga agatcataag aatcctttgc gtagtgttcg atccatcgcg ttgctccctt
     2341 atagatgctt tttaccgaaa acggacgcat gagagtttgc atgatgggcg ggatgagttt
     2401 cttgcgtccc atgttgaact ggaagccgtt gtcacgggac agttcaatgc aaaactcaac
     2461 gagatacgct gtaaattcat ttgagtaaca ggtccatgga ttcctcaaaa atctggaagt
     2521 gacatggtag gaacgatcgg gaatacgctc gttcagaagt ttcaccagcg tttcgacaac
     2581 ctcttctcct gtctgagtgc cttgtgtttg ggtcacatat tcttcaaggt gctcgacgtt
     2641 cgaccttatg gatactccag tcatatccct gatagccacg ccgtcgacgt ccttgccgaa
     2701 tggccgaaac aacatcagtg gacgttcaag aatacggcga gtctttggga gaaactcaaa
     2761 ctctccgggg acgaggagat ggtccgtgtc tgccattcga tgcatctcta ttgcaagggc
     2821 aggcgatttc ttatcgtgtt tggttcaatc agagaacagt aaaatctaaa gccttatcag
     2881 attcaaaagt tgatgcgaag gctcatcacg ccagcatgta ggattgtctc ccaagtgcca
     2941 ttcactatgg gtcgtatgtc gtttgttatg gtgcgcgatt cgtagatctg gttcttgtaa
     3001 gccagatcga ggcctatact ttttatactc gtgagaccaa ccaggtggtt ctggcatttg
     3061 ataaacccga agaatttccc ctgtcctttg caaaggaatc cacatcccac tgaaaaggaa
     3121 ttataatttg cgtctggaac tgccggttcg aatgtgttct ctggtacggg ggtttgcgaa
     3181 cggatatatc cgaaccgtgc agaaatgtcc cattcaggga gaatggaaga gccgatccac
     3241 ttatactctg ttccagtatg gataatgaac actcctttcc agttgcgagg ctctggcaat
     3301 attgctccat tgcttagctg aatgtccact tgcttgaagg tgtgccagtt ggcgtagtcg
     3361 acatcgacct ccagtttcca ttctcgattc ccgtcgcgaa tgggccaata ggctacagcg
     3421 ccagtgacta tctcaggaag tgtgagatca actctggcat tcgcgagtag ccccccattc
     3481 atgagaaagt caccatgaaa attcaacgtc gcttggcttc gatagacgaa tgccaagttg
     3541 agacggggtt tctcatccga gcgccatggc gtgaggagca ctccaatgtt gtaaccaatg
     3601 gcagtatctt tcgcatttaa ctcgagattc gtactgggag gaacaaaagc attgatctga
     3661 acaccacctg ctccagcaaa actcgtgaag gtatagatat ctagacctcc accaattgag
     3721 agatagtcat tgaatcgata agcaaaagta ggcttgatgt cgatcagggg tagggtagcc
     3781 cttgtgtcca aagccgagaa tggaacttct ctgggataat tgatgatgag cccatatggc
     3841 gaattgatgc caaggccaat actgaagttc tttaagaaat caatgttgag cttgccgaag
     3901 ttggcagtca aataaaagtt gattggtgga ggaaaggcaa tcgcaccatc cagattggct
     3961 cgatatcgcg cgccggtagg ggctgtgtaa tcgtaatggc ctccgaccag cagtgtgcct
     4021 ccataaaatt gcatacctga aagttgggtc attcctgccg gattatagtg tatcgccgag
     4081 gggtcatcgg cctgcgcagc aaaggcggtc ccttggcccg aagctgcggc accttggtcc
     4141 aaaatgcgaa atgcctcact gtgagatgga aatggg
//
LOCUS       DATNDD010000179         4249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_586448, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000179 DATNDD010000000
VERSION     DATNDD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586448"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VN647_04255"
     CDS             complement(<1..654)
                     /locus_tag="VN647_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWG96277.1"
                     /translation="MFKNREEAGRRLVERLTRYRGDPTAIILALPRGGVMVGYQLSLG
                     LHFPLDVFIARKLGAPDNPEYALGAVGETGTIYLNPDALAVYNLSGDEMEALAQTQQR
                     EVARQQDLYRQGRQLPTLIDRTVVLVDDGIATGSTFFASAKSIKHLKPRRLIGAVPVG
                     PVETIREACSQVDELIVLATPDPFWAVGNHYVDFAQINDADVVEYLKLAEEAMRERSQ
                     "
     gene            1176..1388
                     /locus_tag="VN647_04260"
     CDS             1176..1388
                     /locus_tag="VN647_04260"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HWG96278.1"
                     /translation="MKVHYYSDAGSPYIDLSERSSVESRELSEGVMLDDDPSGRVVGI
                     EIDNAADKVELQNGSSAGFTIRSKDI"
     gene            1408..1725
                     /locus_tag="VN647_04265"
     CDS             1408..1725
                     /locus_tag="VN647_04265"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HWG96279.1"
                     /translation="MNDGDGMLFVAHWTHTPENCPGRSNEGATMLNEFWAKRDLAAKK
                     GVKILSAYVAATEHTYYITVQAKNYQSLLEFFEPLAPTQTGAIHPVTTMDEWTKHIDP
                     KRR"
     gene            complement(1857..2063)
                     /locus_tag="VN647_04270"
     CDS             complement(1857..2063)
                     /locus_tag="VN647_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96280.1"
                     /translation="MSIERKREEERLITQLSLEPNEQDILIWALQGAISDLGGEIADT
                     ENQEFREDLKQRKTVLQNILRRLR"
     gene            complement(2132..2674)
                     /locus_tag="VN647_04275"
     CDS             complement(2132..2674)
                     /locus_tag="VN647_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWG96281.1"
                     /translation="MEAVRETLTQVLRILDDPSLKTSAKQAQRRHMLEEIAAGRFDYA
                     EMSRRVLGTYWKPLTGTQRKEFVEVYKGFLSDKYAGRIEDYSGRKQDVGYLTERIEGS
                     YAEVRTELRSDKTTLPMDYRLLMTDGRWSAYDIIIDGVSLVSNYRSQFQKIIRERSYE
                     ELVNKLRERSLPAENTKRKP"
     gene            complement(2822..3022)
                     /locus_tag="VN647_04280"
     CDS             complement(2822..3022)
                     /locus_tag="VN647_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWG96282.1"
                     /translation="MSVAKIIELSAESITSFEDAVVQGIAKASKTIHGIKSAWVKEQH
                     VVVENNKVALYRVDLKVTFVLD"
     gene            3603..>4249
                     /locus_tag="VN647_04285"
     CDS             3603..>4249
                     /locus_tag="VN647_04285"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HWG96283.1"
                     /translation="MDKGASVVRRVRLLRIMVGLLPLLWVPVGLGQTTSITSSGMNTH
                     VSHTTGQQNYDITGGTRPGNGTNLFHSFGDFSVGAQDVARFVNDAHLPTTNILSRVTG
                     GNPSNIFGEINTTNFPGANLYLINPAGVLFGPTAMLNVSGSVHVSTADYLRLADGLRF
                     NAVPGPQDTLLSQAPVVAFGFLNAHPHPITVQGSQLTVSDGKSLSLVGGDMAITSG"
BASE COUNT          975 a         1151 c         1111 g         1012 t
ORIGIN      
        1 ctgcgatctc tcgcgcatcg cttcctcagc cagctttagg tactccacca catcggcatc
       61 attgatctgt gcaaagtcga catagtggtt accgacggcc cagaacgggt ccggcgtggc
      121 aaggactatc aactcgtcca cctggctgca agcttctcgt atcgtctcca ctgggccgac
      181 tggaacggct ccaatcaggc ggcgcggctt caggtgcttg atggacttgg ccgaggcgaa
      241 gaatgtggaa ccggtagcga tgccgtcatc cacgagaaca acagttcggt caatgagcgt
      301 agggagctgt cggccctgtc gatagagatc ttgctgtcga gccacctctc gctgttgtgt
      361 ctgagccagg gcctccattt catcaccgga aagattatag acggcgagtg cgtcagggtt
      421 cagatagatc gtccctgttt cacccaccgc gcctagcgca tattcgggat tatcaggtgc
      481 tcccaacttt cgagcgatga agacatccag tggaaaatgc agccccagac tcagctgata
      541 accgaccatt accccgccgc gtggaagcgc caaaataatg gcggtcggat cgccgcggta
      601 tcgggtcaat cgctcaacga gccgacgacc ggcctcttct cgattcttga acattatacg
      661 gttctcttcc atttggattc acgggtctac atcaggccgc ttgtcacgcc gactcctatg
      721 aagaacattg ccatcaagat caggaactgt gagtagcagg tggtggtgcg gtctggactc
      781 agtctgatct caacgaactt cccagggaat ttcgaggtaa cgaggatgac gaaacatgat
      841 accttccttt ccccaatgaa actggaatct catttcaatg tttgtgcatc gatagtgcca
      901 atacctcgga tatgagcatc tatgagaaaa tgcagtgttc caggttcaat taggctggag
      961 gggtctgtgg cattacggga cagtggcatt cctgagactg ttgcctcttt caacatccca
     1021 gcgagtaagt cccgataaga gccgagcgat atggctgtcg aattgtttct attcttgtgg
     1081 ctctgcgtca cgaagagcaa tgaatgggat agccatgatt ccatgccagt ctcgctatgg
     1141 agagaccatc cggacggctc aagaaggttt cactcatgaa ggtccactat tattcggatg
     1201 ccggctcgcc ctatattgat ctttctgaaa gatcaagcgt agaaagtcga gaactttccg
     1261 agggcgtcat gctcgatgac gatccgagcg gacgggtggt gggtattgag atcgacaatg
     1321 ccgccgacaa ggtcgaatta caaaacggat cctcagcagg cttcacgata agatcgaaag
     1381 acatatgatc aaggaggaag gtgaattatg aatgacggcg atgggatgtt gtttgtagct
     1441 cattggacgc acacgcctga gaattgcccg ggaagaagta acgagggagc cacgatgctc
     1501 aatgagttct gggccaagcg ggaccttgcc gccaagaaag gcgtgaagat tctgagtgcc
     1561 tatgtcgccg ccacggaaca tacctactac atcaccgttc aggccaagaa ctatcaatcc
     1621 ctcttggagt ttttcgaacc gttggcgccg acccaaaccg gcgcaattca tccggttacg
     1681 acgatggatg aatggacgaa gcacattgat ccgaagagaa ggtgatgagt gaccctttaa
     1741 cgaaaccggg catcaatgga tcgcgtctga tgcactaggc aacacagaag cactgcgaag
     1801 gtccgaagcc ggccaacatg agcatacagc cgactccggc ggaccggcat ccggctttat
     1861 cgcaagcgcc gcaagatatt ctgtaagaca gtcttccgtt gcttaaggtc ttcacggaat
     1921 tcttggttct ccgtgtccgc tatttctcca ccgagatcag atatggcgcc ctgaagcgcc
     1981 cagatcaaga tatcctgttc gttcggctcc agcgatagct gcgtgataag tctctcctct
     2041 tctcgcttac gctctataga catgtgccgc cgccttttct acaggatgta tgccgaatta
     2101 cacaagcacc tgtgagtgct ccatgctctg gtcatggttt cctcttcgtg ttctccgcag
     2161 gaagggatcg ttcgcgcagt ttgttgacca gctcctcata cgagcgctcg cggatgatct
     2221 tctgaaactg gctccgatag ttactcacca aactgacgcc gtctatgatg atgtcataag
     2281 ccgaccaccg cccatctgtc atcaagaggc ggtaatccat cggaagcgtc gttttatccg
     2341 accgcaactc ggttctcact tccgcgtacg acccttcgat ccgttccgtc agatagccca
     2401 catcttgttt ccgtccgcta tagtcttcga ttcttccggc atacttatcg gagagaaatc
     2461 ccttgtacac ctcgacgaac tccttccgtt gagtccctgt cagcggcttc caataggtcc
     2521 ccaacactcg cctcgacatt tccgcgtaat caaatctgcc ggcggcgatt tcttcgagca
     2581 tatgacgacg ctgcgcctgt ttggccgaag ttttcaggga agggtcatcg aggatgcgga
     2641 ggacttgagt aagcgtctcg cgcaccgctt ccatcgggct ttccgcccct tgcgaagaag
     2701 cggtgacgac aaagcaccac agaaaagcgc atgcaaaggc cgcaggccac agtgatcctc
     2761 ttgcagcttg ccgactcctt ccgggccact gaggcatgaa gtgtaggttc tccgtaacgc
     2821 gtcaatccaa tacgaacgtc acctttagat ctacacgata caaagccacc ttgttgtttt
     2881 caaccacgac atgttgctcc ttcacccaag ccgatttgat gccgtggatg gtcttggatg
     2941 ctttggctat tccttggacg acggcatctt caaagctcgt gatcgactcg gcgctaagct
     3001 cgataatttt agcaacggac ataaggctcc tccctttcat gtcatgcgca tgtgcgcggt
     3061 cgatgactca tcaatcttcg gctcgaacca atatattaag cacgtagcaa ggttgaggcc
     3121 agggatcgtt cggccggcaa ctgtgcatag aaaggagatg tggacggatt tggttgccct
     3181 atattgcctc aacatgtgcc tatcaccagt gggcaatgtg ccccaagacg agcctctgac
     3241 acttcttacg ttccgcctgc tctcctgcgt gaatcagact gcaagcgcgc ttacgtagcc
     3301 actggagaag gtcctgtcta gtagcgagtt tgcttaagca tagatcagta gctatactag
     3361 tacccatttt ttagccaacc caaaagagag gcgattttct cctgtgttcg gggcttggag
     3421 gatgatgatc atgacaagat aatcggcgaa gtcagaggtc taggtgacag aatagcttca
     3481 atcgatacgc acacataact ccgtaaaaga acgacctgca tttgtcggcg gagcatcggg
     3541 tttttgtagc ggccagaatg gggtaagtgg ctccgttcgg tcattttgaa aggagagatg
     3601 ttatggacaa gggagcaagt gtcgtgcgac gagtgcggct gctcagaatc atggtcgggc
     3661 tgcttccgct cctctgggtg ccggtcggtc tagggcaaac cacttcgatt acgtcctctg
     3721 gaatgaacac ccacgtttca catacgaccg gtcaacagaa ctacgacatt acgggcggca
     3781 cgaggccggg caatggcacg aatctctttc acagctttgg tgacttcagc gtcggggcgc
     3841 aggacgtcgc gcgctttgtg aatgatgcgc atctgcccac cacaaatatt ctcagtcgtg
     3901 tgaccggcgg aaatccttca aatatctttg gcgagatcaa tacgacgaat tttcccgggg
     3961 cgaacctcta tctcatcaac ccggccggcg tgctatttgg ccccacggcc atgttgaatg
     4021 tgagtggatc agtgcatgtc tcgacggccg attacctccg cttagccgat ggtctgcgct
     4081 ttaatgccgt gccaggccct caggatacgc ttctctcaca ggctcccgtg gtcgcctttg
     4141 gatttttgaa tgcccatccc catccgatca ccgtccaggg cagccagctg actgtgtcag
     4201 acggcaagag tctttcgctg gtcgggggcg atatggcaat caccagcgg
//
LOCUS       DATNDD010000180         5544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3890210, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000180 DATNDD010000000
VERSION     DATNDD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890210"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VN647_04290"
     CDS             complement(<1..1238)
                     /locus_tag="VN647_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96284.1"
                     /translation="MSHLCKEKENERTLHGCTDGPSRQIEKEARHVRHYQKLVSTVCV
                     IIAFLIVGCDGRGGESVGPTLFAAIAPTTYSAYTGTDAKQIPHAPALGPANSIIQDPT
                     FGSRILRVTDAHTHGGDSLIPEYAGFFRTWNADSTALKLHGPHGTSFWLEFHASSFKV
                     GDGSARPTLHPLSFNVKWEWSAVDPDIIYFINGNQLAKYNKATHVVTYLGPHQSVPLA
                     YHVLVVGKDAWICAAAGSAGQNTYTQIYCLNPHDPSQHKFIDILKKTINGIAQHDPHW
                     PTSAAGQTIGIHAMYGSAAGAWLDVGFVHHSWGVNGDSVFNLSTNTWSLMTNANMYSS
                     GHSSIGAKFVNGCGSINGMYSGGACLRDPSNLMDATQYTFIMQPPSTATGWHDGEHSS
                     WFNASTNPHAPVLFSRYNIST"
     gene            complement(1551..1835)
                     /locus_tag="VN647_04295"
     CDS             complement(1551..1835)
                     /locus_tag="VN647_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96285.1"
                     /translation="MPVESIESQSLYIFLDEGGNLDFSPNGTKFFTVSTFTKCRPFAL
                     DADLLALKYDLLEEGLDLDCFHASEDRQLVRNRVFSLIGRHLAQFLPTHA"
     gene            complement(2128..3537)
                     /locus_tag="VN647_04300"
     CDS             complement(2128..3537)
                     /locus_tag="VN647_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96286.1"
                     /translation="MSHKMMQSDRYGFCRPLKKPCAVISLLIIALLGFGSSLFAASAP
                     TTYSAYTGTDAKATPPAPALGPANSVINDPTFGSRILRVTDSNTASGGSFIPSDAGYF
                     RTWNANSTALKLVNPHGFSYWLEFNSSTFKVGDGSPQPSLHPLSFYWTWEWSAVDPDV
                     IYFLNGNQIAKYNKVTSLVTNLGGPPNGDPVTYRVAVVGTDKWVCSAAGPGTQNTYTE
                     IYCVNPNNTSQVKFIDVVNQTINGVKQRDRNWPTSAAGQTIGIHSLYGSAGGAWLGVN
                     FHQQSWGANGDSVLNLNNNDWSLVTNADTHWSGHSSIGNGKYVNGSGSINGNDSRGAL
                     LRDPNNLMNTNQYTFIMQPPSTVGWYDGEHSSWFNSSTNPKAPVLFSRYSITAPPGTL
                     TWYGEIIAAATDGSNRVWRFAHNHNGGLGGYYGSAFAQISNDGRWALFSSYWDGTLGA
                     SPGDFGFSTRIDTFIVELK"
     gene            complement(3651..3815)
                     /locus_tag="VN647_04305"
     CDS             complement(3651..3815)
                     /locus_tag="VN647_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96287.1"
                     /translation="MKKRKNPHAVALSKLGAKKGGKTRAARLSPEQRSASARLAAQAR
                     WSKKGKRSSS"
     gene            complement(4045..4320)
                     /locus_tag="VN647_04310"
     CDS             complement(4045..4320)
                     /locus_tag="VN647_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96288.1"
                     /translation="MGPPPILHIWSGVYGFFGKSGISDISFIGQSIGQKCDVEAGHGF
                     AELLVPSEATVAVAARHRFTAEDVAAGAAETMPQQCGPDLERHTEVL"
     gene            complement(4309..4386)
                     /locus_tag="VN647_04315"
     tRNA            complement(4309..4386)
                     /locus_tag="VN647_04315"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4348..4350),aa:Glu,seq:ctc)
     gene            4580..5137
                     /locus_tag="VN647_04320"
     CDS             4580..5137
                     /locus_tag="VN647_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96289.1"
                     /translation="MQNPFQTDANMDANMMDVGEAAETIVEEEKPEAGLELHVGSNIF
                     RATNGVIKFQGKEQIVLEVQPDPPSLLLTMDFYDEAGRRIGHLRRNQLSAVGASRFAV
                     GVTTPADPTLDDPLTVTVSERATGSTVVEVYLFHQRKIRFTTAHFHTHKGEVVSISPH
                     YCRLGTGLTMFGDVVEGRGGTAAIG"
     gene            5223..>5544
                     /locus_tag="VN647_04325"
     CDS             5223..>5544
                     /locus_tag="VN647_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96290.1"
                     /translation="MSEIIKPSVQAFLVCDQVIEDSVTKKKNLIGLFTHLQASSFPFQ
                     HQQMGLYFCLTDAEGTYRFDIDLIYLNSEQLVCRATLPNIVIADRLQISDFGINIPSL
                     IFPAP"
BASE COUNT         1259 a         1519 c         1556 g         1210 t
ORIGIN      
        1 gtactgatgt tatatcgtga aaacagcacg ggcgcgtggg gattcgttga cgcattgaac
       61 cacgaactgt gttcgccgtc atgccatccc gtggcggtcg acgggggctg catgataaag
      121 gtgtactgcg tggcatccat gaggttactc ggatctcgaa ggcaagcgcc tccagagtac
      181 atcccattga tcgatccaca gccgttgaca aatttggcgc ctatagacga gtgtccgctc
      241 gaatacatat tggcattcgt catcagcgac caggtattgg tcgaaagatt gaacacagaa
      301 tcgccattga ctccccagct gtggtgcacg aaacccacgt ccagccaggc gccggctgca
      361 ctcccataca ttgcatgaat cccgatggtt tggccggcag ccgaggttgg ccagtgagga
      421 tcgtgttggg caataccgtt gatggtcttc ttcaagatat caatgaattt gtgctgcgat
      481 ggatcatggg gattcagaca gtaaatctgt gtataggtat tttggcctgc tgatcctgcc
      541 gccgcacaga tccaagcgtc tttgccgaca accaggacat gataggcgag cggaacgctc
      601 tgatgcgggc cgaggtaagt gaccacatgc gtggccttgt tgtatttcgc cagttggttc
      661 ccgtttataa agtagataat gtcgggatca acggcggacc attcccattt cacgttgaag
      721 ctcaggggat gcaaggttgg tcgggcagac ccatccccga ccttgaaact tgaggcgtgg
      781 aactcaagcc aaaaggatgt cccgtgggga ccatggagct tcagcgccgt tgaatcggca
      841 ttccaggtcc gaaagaagcc agcatattcg ggaatcaagg aatcgccgcc atgggtatgg
      901 gcatcggtca ctcgcaggat gcgcgagccg aacgtcggat cctggataat cgagttagcc
      961 ggaccgaggg ctggagcgtg cgggatctgt ttggcatcgg ttcccgtata ggcagaatag
     1021 gtggtgggag cgatggcggc gaaaagtgtt gggccgacac tctcaccacc ccgcccatca
     1081 caaccaacta tcaggaacgc gatgatcaca catacggtgc tcactaactt ttggtagtgc
     1141 ctgacgtggc gcgcctcctt ctcgatttgc cgagaggggc cgtccgtgca accgtgcaac
     1201 gttcgttcgt tttctttttc tttacataga tgagacacgg aatcctccaa tgctgcaggt
     1261 ggacacaatg ggcctacgag tcgttgtggc gatagcgcac ctacgcgctg acccattggt
     1321 tatgtacggt taaagaagtc gggagattta ggcagaagga ggatcggttt gggacgggct
     1381 gacagcgtga tcatcttctc ttaagtagag cgggtacatt ctggacctag tactcggtta
     1441 agctaaattc tatcgatcaa ccgaaaatca agcgatattc aagcagatcg caacacttca
     1501 gagtggcggg agtatacgga gccgaagctg agtcacaact agtgaaaccc ctaggcatgg
     1561 gttggcagga attgggcgag atgcctcccg atgagtgaaa agacgcgatt ccggaccagt
     1621 tgcctatctt ctgacgcatg gaagcaatca aggtcgagac cctcttctaa gagatcatac
     1681 ttgagtgcaa ggaggtccgc gtctaaggca aagggccggc atttcgtaaa cgtgctgacg
     1741 gtgaaaaact tcgtcccgtt tggtgagaag tcgagattgc cgccttcgtc aagaaagata
     1801 tagagggact gggattcgat agactccacg ggcaccggat ttccatttcg atggatcggg
     1861 agacaaggag tcttctaagt gccttctccc aggaagtcaa accttcctca tcctgtcctc
     1921 cctatgcgct ccttcctagc ctgctcacag atcccttcaa tttctcgaac cctcgccgga
     1981 tccggtggag gtccgcagtc gaatagcacg gtccagtggt cggctttgcg atgagtcagc
     2041 ctggcgatcg caagatgacg aagcatggcc ggtgatattc tgaccggccg gccagccact
     2101 ccacgccagg cggtaaagaa gaccttgtta tttcagctca acgatgaatg tatcgatacg
     2161 agttgaaaac ccaaagtcgc ccggcgatgc gcccagcgtg ccgtcccaat agctggagaa
     2221 cagcgcccaa cggccatcat tggaaatctg cgcaaacgcg ctcccatagt agcctccaag
     2281 cccgccattg tgattgtgcg cgaatcgcca gaccctattg gagccatccg tcgccgcagc
     2341 aatgatctct ccataccagg tcagtgttcc gggaggcgca gtgatgctat accgcgaaaa
     2401 gagcacgggc gctttgggat tcgtcgacga attgaaccag gaactatgtt cgccgtcata
     2461 ccagcccacg gtcgacggcg gctgcatgat gaaggtgtac tggttggtat tcatgaggtt
     2521 gttcggatct cggaggagag cgcctcgaga gtcattgcca ttgatcgatc ctgagccgtt
     2581 gacatatttg ccgttgccta tggaggagtg tccagaccaa tgggtatcgg cgttcgtgac
     2641 cagcgaccag tcattgttgt tcaaattgag cacagaatcg ccattggctc cccagctctg
     2701 ttgatggaag tttacgccaa gccaggcccc accggcactg ccatacagcg aatgaatgcc
     2761 gatggtttgg ccggcagccg aggtcggcca gttacgatcc ctttgcttaa ccccattgat
     2821 cgtttggttc acgacatcaa tgaatttgac ctgcgaagtg ttgttaggat tcacgcaata
     2881 aatctctgta tacgtattct gcgttcccgg tcccgcggcc gagcagaccc acttgtctgt
     2941 tccgacgacc gcgacacgat aggtgaccgg atcgccgtta ggcggacctc cgaggttggt
     3001 gaccagagaa gtgactttgt tgtatttcgc tatttgattc ccgttcagaa agtaaataac
     3061 gtccgggtcg accgccgacc attcccaagt ccagtaaaag ctcaacggat gcaagcttgg
     3121 ctgtggggac ccatcgccga ctttgaaagt tgaggagttg aactcgagcc aataggagaa
     3181 tccatgggga ttcacgagct tcagcgcggt ggaattggcg ttccaggttc gaaagtagcc
     3241 agcatctgag ggaatgaagg aaccgccgct cgcggtattg ctatcggtta ctcgtagaat
     3301 acgcgagccg aaggtcggat cattgatgac cgagttagcc ggaccgaggg ccggagcggg
     3361 tggggttgcc ttagcatcag ttcccgtgta ggcggaatag gtcgtcggcg ccgaggctgc
     3421 aaaaagcgat gagccgaaac ccagtaacgc aatgattagc agcgagatta cggcgcacgg
     3481 cttcttgagt ggccgacaga agccatacct gtcactctgc atcattttat gagacatgac
     3541 atcctcccca tgcacggtta atccaaaaag ctgagacacg cccagtggga catattggca
     3601 caatagcacc ggcggtcggc atgccgctga tctactagtt gcctgctccg ttaggaactg
     3661 gaccgcttcc ctttcttgct ccaccgagct tgagcggcca gccgagccga cgccgaccgc
     3721 tgttcaggag acagccgagc tgcgcgcgtc ttcccacctt tcttggctcc cagtttcgag
     3781 agagcgactg catgcggatt cttacgtttt ttcatggcgc cacagtatgc ccagccgagc
     3841 aggcacggtc aacacgttga cacatgcgtg cacgtgcacg cataataatg cctaacagcc
     3901 ctacggagcg agatcgccca tgatgggaaa accttcaggc gatggaatgc gggcgcgttt
     3961 gggaccttct ggcgacgttt gacgatcgag gccaaggtac aaggtctcca cttccatgat
     4021 ttgcgccact cgctcacgac ctggctacag aacctcggtg tgccgctcga ggtcagggcc
     4081 gcattgttgg ggcatcgtct ccgcagcacc ggcagcgacg tcctcggcgg tgaagcgatg
     4141 acgagccgct acagccacgg tggcttcgga tggaaccagc agctccgcga agccgtgacc
     4201 cgcctccaca tcgcactttt gtcctatgga ttgtccgatg aacgagatgt ccgagatgcc
     4261 gctctttcca aaaaagccgt aaacgccgct ccagatgtgg agaattggtg gtggtcccaa
     4321 cgggatttga acccgtgtct gagccttgag agggctccgt cctaggccag gctagacgat
     4381 gggaccaaag gtgcagactt ggaacggagt gctgacggta ccatgagcct agtggggttt
     4441 tcaattccat cgccttgaga cggaccggaa tttttttggc gccagcatct tgacagatcc
     4501 gattgcttgg cctatttttt cgcaggctat cacgtcccga cacgactcct cactcgcgct
     4561 cacgcaaaag gctcgcctca tgcagaatcc atttcagacc gacgccaata tggatgccaa
     4621 catgatggac gtcggcgaag ccgccgaaac aatcgtcgag gaagaaaagc cagaagccgg
     4681 attggaactg cacgtcggct ccaacatctt tcgagccacg aacggcgtca tcaagtttca
     4741 gggcaaagaa cagattgtgt tggaagtcca gcccgacccg ccgtcgttgc tgttgacgat
     4801 ggatttctac gatgaggcgg ggcggcgcat cggccatttg agacgcaacc aactctcggc
     4861 cgtcggagcg agtcgatttg cagtgggcgt gaccactcct gctgatccaa ccctcgatga
     4921 tcctctcacg gtcacggttt ccgaacgggc aaccggcagc acggtggtgg aggtgtattt
     4981 gttccaccaa cggaaaatcc gatttacgac tgcacatttt catacccaca agggcgaagt
     5041 ggtgtcgatc agcccccact actgccgcct gggcaccggt ttgacgatgt tcggtgatgt
     5101 ggtcgaaggc cgcggtggga ccgcggcaat cggttagccc accgcccggc gtccccatct
     5161 ccctcgccca cggaaaggca tatccctttg acagcctaca agactcccgt agaataggca
     5221 cgatgtctga aatcatcaaa cccagcgtgc aggcgttcct tgtctgcgac caagtcatcg
     5281 aagacagcgt gaccaagaaa aagaacctga tcggcctgtt cacgcatctg caggcctcga
     5341 gctttccatt ccaacaccaa cagatgggct tgtacttctg cctgaccgac gcggaaggca
     5401 cttaccggtt cgacatcgac ctcatctacc tcaacagcga acaactcgtc tgccgcgcta
     5461 cgctcccgaa tatcgtcatc gcggaccgtc tgcagatctc ggactttggg attaatatcc
     5521 cgtcgctgat ctttccggcc cccg
//
LOCUS       DATNDD010000181         4164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_333396, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000181 DATNDD010000000
VERSION     DATNDD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333396"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VN647_04330"
     CDS             complement(<1..270)
                     /locus_tag="VN647_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96291.1"
                     /translation="MSGLMSADDMFSKAEAAAVAATGPDEKALQIDYDALREKFRKAL
                     GDRKVALCHVNKFLPEGYEDQGRFNLILLTAGNVVFDIVIGDSYFR"
     gene            complement(380..1744)
                     /locus_tag="VN647_04335"
     CDS             complement(380..1744)
                     /locus_tag="VN647_04335"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWG96292.1"
                     /translation="MDSDFLPQGVAVGDVGSQSALLWLRTDGPASVQIEWATVSRWER
                     ASKLATVVAPVPRTASIITTAETDYTLIIPLEGLIPATRYRYHVLVGSADQNARQLPA
                     SLAAMGEFTTLPDDKTSAAVTFVWSGDLGGQKRCRQGVGGYPIFDIIRRQHPDFFLFL
                     GDTIYSDDICPSPPNEPGADFKATTLQTYRVRHRYQRGAEALRRFLETTPVYVVWDDH
                     DVRNNFSGPYDEQMPAGRQALREYWPIASPAHDPHRLYRHVRYGADLELFILDTRQYR
                     SRNADPDGPDKTMLGATQLAWLLDGLRTSTATWKAIVTSVPLSMPKGGDVNVPGYDGW
                     AGGLDGTGFERERRVIVDTILDHKLKNVVFLAGDVHLAQANAYDPDRDGVIDFHEYVV
                     GPLSARHGRLTATDDGLHQTQLFYESGFDNFGLARVTKNEFAVAVVDEAGTKRFSHVV
                     PARP"
     gene            1879..2208
                     /locus_tag="VN647_04340"
     CDS             1879..2208
                     /locus_tag="VN647_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96293.1"
                     /translation="MKLTGSSSEGVAGLKIGTADRHTEQVHRRTKMLDSRCCAPNCLE
                     APYAPAGTRSICKDHFLNFLTWRRRRGVQMFHTYAGMTMEERDTIAAEWMKTIRTDEL
                     PAPAPKL"
     gene            complement(2222..2668)
                     /locus_tag="VN647_04345"
     CDS             complement(2222..2668)
                     /locus_tag="VN647_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWG96294.1"
                     /translation="MAYTVRRAILHDLNQLVLLFDAYRRFYGQQSDIIVARQFLNDRL
                     TRNESIVLVAENDSGAAVGFVQLYPTFSSILAAPMYVLSDLFVIPEARRRGMGTQLLA
                     SAAEAARTAGAVRLELATAITNISAQRLYEALGWRKDDFYLYGISL"
     gene            complement(2949..3341)
                     /locus_tag="VN647_04350"
     CDS             complement(2949..3341)
                     /locus_tag="VN647_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWG96295.1"
                     /translation="MLKQRIDAVTKANQEFYDAFESLDIAKMDEIWAHQEYVTCIHPG
                     WTIRSGWPAVRDSWVLIFNNTFSMKFELTDVMVQVAGDMAWVICVENLTTQQSDEPQQ
                     AKVLATNLFELIGDEWLMIHHHGSPVMG"
     gene            complement(3430..3771)
                     /locus_tag="VN647_04355"
     CDS             complement(3430..3771)
                     /locus_tag="VN647_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96296.1"
                     /translation="MNVKGFIIEDDQGREFVLLCEQPFGRSGRTFSLRGWQRLRLVPL
                     RYTERELRKIIGGVLATLNDLFKSLPRLLEHVSDAASDHAGSATTFTRKTPALRKVTR
                     REENTVRSEWP"
BASE COUNT          939 a         1242 c         1095 g          888 t
ORIGIN      
        1 ccggaaataa gaatcaccga tgacgatgtc gaacacgaca ttgccagcgg tcagtagtat
       61 caggttgaac cgaccttgat cctcgtatcc ttccggtaaa aacttattga cgtggcaaag
      121 cgccaccttt cgatcaccca acgccttgcg aaatttctct ctcaatgcgt cataatcgat
      181 ttgcagggcc ttttcatccg gcccagtcgc agctacggcc gccgcttccg ctttcgagaa
      241 catatcatcg gcggacatca atcctgacat gacgtgtact cccttgctat cgttgcagtg
      301 aactgagcaa tgacctccgg agccgtacgg taaccggcca tctgccgcaa ttgcaagtgg
      361 cttgaacggt gagaacggct tacggcctgg ccggcacaac gtgagaaaat cgcttcgtac
      421 cggcctcatc caccactgcg acggcgaact cgttcttggt gacgcgagcg agaccgaaat
      481 tgtcgaatcc tgactcgtaa aagagctgcg tctgatgcag tccgtcatcc gtcgccgtca
      541 atctcccatg ccgggcggaa agaggtccga cgacgtattc gtgaaaatcg atcacgccgt
      601 cccggtcggg atcataggcg ttcgcctgtg cgaggtgtac atcacccgca agaaacacca
      661 catttttcag cttgtgatcc agaatagtat cgacgattac tcgacgttcc cgttcaaagc
      721 cggtgccgtc cagccctccc gcccagccat cgtagccggg aacgttgaca tcacccccct
      781 tcggcatcga gagcggaacg gaggtcacga tcgctttcca ggtcgcagtt gaagtgcgaa
      841 ggccatcgag caaccacgcc aattgtgtcg ctccgagcat ggtcttgtca ggaccatccg
      901 gatcggcatt acggctccga tattgcctgg tatccagaat aaagagttcg aggtcggcac
      961 cataacgtac atgccgatag agacgatgcg gatcgtgtgc cggagaagcg atgggccagt
     1021 actcccgcaa tgcctgccgc ccggccggca tctgttcgtc atacgggccg gaaaaattgt
     1081 tcctcacgtc atgatcatcc cacaccacat agaccggcgt ggtttccaag aatcggcgca
     1141 gagcctccgc accacgttga tagcggtgac gtacccgata ggtctgcagc gtggtcgctt
     1201 tgaagtccgc acccggttcg ttcggcggag agggacagat gtcgtcgcta taaatcgtat
     1261 ccccgagaaa cagaaagaag tcgggatgtt gtcgacggat gatgtcgaag atcggatagc
     1321 cgccgacacc ttggcgacag cgcttctgac caccaagatc tccgctccag acaaacgtca
     1381 ccgcagccga agtcttatca tccggcaagg tggtaaattc gcccatggcc gcaagacttg
     1441 cagggagctg tcttgcgttc tggtcggcag aaccgaccag aacatgatac cgatagcgcg
     1501 tggcggggat cagaccttcc aatgggataa tgagggtata atccgtctct gcggtcgtaa
     1561 tgatggacgc cgttctcggc accggagcca cgactgtggc aagtttcgag gctcgttccc
     1621 atcgtgaaac agtggcccac tcaatctgaa ccgaagcagg tccgtcggta cgcaaccaga
     1681 gcaacgcgct ttgcgaaccg acatcgccga ccgcaacacc ttgaggcaag aagtccgagt
     1741 ccatgaacgg ctttccctcg aacgaactgc tggatcgaac gctctcattt gaaaatacaa
     1801 cgcagccgac gaaagaggag ccaaggacta gcataccggc cagcaaaagg aacgcgcgca
     1861 tcggggcagt cctaccctat gaagctgaca gggtcaagca gcgaaggcgt cgcgggcttg
     1921 aaaatcggca cagccgaccg gcatactgaa caagttcacc ggaggaccaa gatgttggat
     1981 agtcgctgct gcgcgcctaa ttgcctggag gcaccctatg caccggccgg cacgcgttcc
     2041 atctgcaaag accatttcct caactttctc acgtggcgtc gccgacgtgg cgttcagatg
     2101 ttccacacct acgccggtat gacgatggag gaacgcgata ccatcgccgc tgaatggatg
     2161 aaaaccattc ggacggatga actgcccgcc cctgcaccga aactctgacc tcgtcgctct
     2221 gctacaacga tatgccatac aggtagaagt cgtctttccg ccaccccaac gcttcataga
     2281 gtctttgtgc cgaaatgttg gtgatcgcgg tcgccagttc caaccgaacc gcgccggccg
     2341 tacgagcggc ctcggcagca gacgcgagca attgagttcc catgcctcgc ctcctagcct
     2401 caggtatgac aaacaagtcg ctgagcacat acatcggtgc ggcgagaatg gatgaaaagg
     2461 tggggtacag ttgcacaaag ccgaccgccg ctccactatc attttcagca actaatacaa
     2521 tcgactcgtt ccgcgtaaga cgatcgttca agaattgccg ggcgacaatg atatccgact
     2581 gctgcccata gaaccggcgg taggcgtcga agagcaagac gagttgattg agatcatgca
     2641 gtatcgccct gcgtacggtg tatgccatga gctgagaagt agagcaatcc aaggccaaac
     2701 ttgcccgaaa attttctgat accgtttata cctgcgtaga attcattggt ttgcaccttc
     2761 ccctttggcg ggcggtggtg tggttttctt ctgcgcgcat tgagcgagca ccgccgatcc
     2821 tcttttcctc actctctctt actctttaag ggggcggccg agtggtctcc taactgcgcg
     2881 catccaacga gggccttccc aggccgcgcg ttgggcgagc acgggagacc attcggctgc
     2941 ccccgctctc accccatcac aggcgacccg tggtgatgga tcatcagcca ctcatcgccg
     3001 atgagttcga aaaggttcgt cgcaaggacc ttggcttgtt gcggttcgtc ggactgttga
     3061 gtggtgagat tctcgacgca aatgacccag gccatatctc ctgcgacttg gaccatcacg
     3121 tcggtgagtt cgaacttcat cgagaaggta ttattgaaga tcagcaccca ggaatcgcgg
     3181 acggcgggcc atccggaacg aatcgtccag cccgggtgta tacaggtgac gtattcttga
     3241 tgggcccaga tctcgtccat cttggcgatg tcgagactct caaaggcgtc gtagaattcc
     3301 tggttggctt tcgtgacggc gtcgatacgc tgtttcaaca cggctacgcc tctcagtcag
     3361 cggagccgta tcatactgca agcgagtgca gcgctgcgag gacatttctc aggacacgag
     3421 actcgtaccc tacggccact cgctcctcac cgtattttct tctcgcctgg tgactttcct
     3481 caacgccggt gtcttcctcg tgaacgttgt cgcggagccg gcatgatcag aagccgcgtc
     3541 agagacatgt tcaagcagcc tcgggaggct cttaaataag tcattcagtg tagccaacac
     3601 tccaccgatg atttttcgaa gctcccgttc agtataccgc aacggaacca ggcgcagccg
     3661 ctgccacccg cgaagtgaga aggttctccc cgatcgccca aacggctgtt cgcacaggag
     3721 cacgaattcc cttccctggt catcctcgat aatgaacccc ttgacgttca tacgacctcc
     3781 ctaccccttt tcagaggtat cgtctccaca tatctgttca acggcgtttc gttttcccga
     3841 ctatgttgcg ggaaccgcgg ggacggctcg cactgctctg ttccgtatga tgaccgagtc
     3901 caccacattt ccgcagttca cacagcgcca ggacacccct aggataagtt catcactcgc
     3961 actatgcccc acacgccgtt ccggcatcat ccatccgttg catcgctcac aacccatggc
     4021 cgctcctttc cggcgtctag aaattggatt ctgtttgcct ccgctgttga gggaagagac
     4081 gagccacagt tgggagcaaa tccctgtaca gcgagtcctt cttgagaaat gcgtcggctt
     4141 tcgaatcacg atcctcacac gttt
//
LOCUS       DATNDD010000182         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1242744, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000182 DATNDD010000000
VERSION     DATNDD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242744"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..852)
                     /locus_tag="VN647_04360"
     CDS             complement(10..852)
                     /locus_tag="VN647_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWG96297.1"
                     /translation="MAYELTRHRFLELLRTYQPSSYETFGQGDARFAIPMHHAMKCRD
                     RLHVALKSGLRRLPSKKQTIVDLGPFPGSLLRLLRSLDYTRSAQLFGAGLMVSEGFLR
                     GMQRDAQADILTVNLDPAGGQFQSKGYPEKVPLPDNSVQLVFALEIIEHLMSPVHLLA
                     EAYRLLRSGGHMVLTTPNVTRIGNVFKLLIGRTPNDRLAPPGYNNPDDEWRPHTREYA
                     MHELTEMLRRTGFDIAETRFFLGEDTQECRQSLSQHGIDWVKWPFYVVPHLRGSLLIV
                     GRKP"
     gene            complement(852..2141)
                     /locus_tag="VN647_04365"
     CDS             complement(852..2141)
                     /locus_tag="VN647_04365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWG96298.1"
                     /translation="MFLLSAMVAGLFARTWVILHAPGHAFAADQLFDTLAWNLATLHQ
                     FTFDGVNPAAHVGPLYPLVLAGFYTFVGHRPEWVPMLHVLFDLGTAWCMYQVATALWG
                     SWIGAWTAALVFLYPAYWSYDPRIRSEALLTLLMSVWLWATVVCGQSPSSCRCAFLGL
                     AAGLTILCKPVVLVLALLLAGLLWIDTDRLSQKIGYVIVYGVTCAALVIPWSLRNYVV
                     FHHIIPVSAGVGAGLWMGSDPVSRGSWPMPPEREQAIWESAGITPLPYAHAMYDVQTD
                     RLLREKGMSRIAADPIRYVGLTFTRVWDFWIGNSFYLVNGEGLTHGLQTDAAERGWAV
                     AIYSLIKRLLLMPGLIVVAACSAWVHRKRWRDLLPLYLFPIGLTAGYVPFTVEAGRYA
                     LPVLPCFMMLSVALITHWQAVRSFHLDRHTAPQTTSM"
     gene            complement(2177..2917)
                     /locus_tag="VN647_04370"
     CDS             complement(2177..2917)
                     /locus_tag="VN647_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96299.1"
                     /translation="MERRAAYPTDTQHVVNLSGYVWAARQLGPLKERCVLDLSCGTGY
                     GSDYLGACAGRIVGIDGAPDVVAKCRADYPRSNVAFLAMDACALAFRDASFDCIVSQD
                     TIEHVQDDSCFVSELTRVLKPNGVLVIFTPHGKGRGVIPDDRYHVREYTPDEFKTLLQ
                     PHFSTVRWYGRRQGHRLKAVERSMDTVRNFDPVGLRNCVPRPIRHWLGSVISRAQGGP
                     ALHELVPDDIEYREGVTDDTNLIGVCIK"
     gene            complement(2998..>3456)
                     /locus_tag="VN647_04375"
     CDS             complement(2998..>3456)
                     /locus_tag="VN647_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96300.1"
                     /translation="QDVDWINGACLMVRTDLMRACCGLDEGYFMYFEDVDLCRALHQR
                     GYRIRHLADADIVHLIGRSGERDRTQLNLVWEFSRIRYVEKHFPLFKRWIMKAWIALG
                     AIVRLIGAVGRRPSTVSDCSLDMYVSVVRRLWHWGQPGPLGLAWTRPNRG"
BASE COUNT          845 a         1018 c          908 g          685 t
ORIGIN      
        1 cgaccggctt catggtttcc ttcccactat gagaagactg ccgcgcaaat gcggcacgac
       61 atagaacggc catttcaccc aatcaatgcc gtgctggctt aaggattgtc ggcactcttg
      121 tgtgtcctca ccaagaaaaa accgagtttc cgcgatgtcg aagccggttc gtcgtagcat
      181 ctcggtcagc tcatgcatcg cgtattcgcg ggtatggggc ctccactcat catccggatt
      241 gttgtatccc ggtggagcaa gtcgatcgtt cggcgtccga ccaatcaaca gcttgaagac
      301 attcccgatc cgcgtcacgt tcggtgtcgt cagcaccata tggccacccg atcgcaatag
      361 gcgatacgcc tccgcaagca aatggacggg tgacatcaga tgttcgatga tttccaacgc
      421 aaataccaac tggacggagt tatccggaag cggcaccttc tcgggatatc ctttgctctg
      481 aaactgccca cctgccggat cgagattgac cgtcaggatg tctgcttggg catctcgctg
      541 cataccacgc aaaaaacctt ctgagaccat caaccctgct ccgaacaatt gtgccgaccg
      601 cgtataatcc aggctgcgca agagtcgcaa aaggcttcca ggaaacgggc ctaaatcgac
      661 gatcgtctgt ttcttagacg gcaatctacg caagccactt ttcaaggcca catgcaatcg
      721 gtctcggcat ttcatcgcat ggtgcatggg aatggcaaag cgtgcgtcac cttgtccaaa
      781 cgtctcatag gaggacggct gatacgttct caacagctca aggaagcgat gcctggtcag
      841 ttcgtatgcc atcacatact cgtggtctgt ggagcagtgt gtctatccag gtggaacgat
      901 cgcactgcct gccagtgcgt tatcagagca actgagagca tcatgaagca tggtaatacg
      961 ggcaaggcat agcggccagc ttcgaccgtg aatggaacat agcccgccgt gagtcctatg
     1021 gggaaaagat acagcggcag aaggtcccgc catcgctttc gatgaaccca cgcgctgcat
     1081 gcggcaacca cgattaagcc gggcattaag agcaatcgtt tgatcagact gtagatcgcc
     1141 actgcccatc ctcgctctgc cgcatccgtc tgcaatccat gcgtcagccc ctccccatta
     1201 actaaataga acgaattgcc gatccagaaa tcccacaccc ttgtgaaggt caaaccgaca
     1261 taccggatag gatcggctgc gatccgactc atgcctttct cacgtaacag ccgatctgtt
     1321 tgcacgtcgt acattgcgtg cgcataggga agcggggtga tgccggcact ttcccagatg
     1381 gcctgctctc gctccggcgg catcggccag ctccctcgag agaccggatc gctccccatc
     1441 cataatccag cccccacccc ggccgatacg ggaatgatgt gatgaaatac gacataattt
     1501 cgtagcgacc aagggatgac gagcgcagca caggttacgc cgtatacgat cacatagccg
     1561 atcttttggg aaagacgatc cgtatcgatc cacaggagtc ccgcgagaag cagcgccaga
     1621 accaacacca ccggtttgca gaggatagtt aagccagccg cgaggcctag gaacgcacat
     1681 cgacagctgg atggactttg accacagaca acagtcgccc agagccagac gctcatgagg
     1741 agtgtgagga gcgcttcgct cctgatccgc ggatcatatg accaataggc ggggtagagg
     1801 aacacgagag cggcggtcca agctccgatc catgaacccc acaatgcggt cgcgacttga
     1861 tacatgcacc atgcggtgcc gagatcgaag agcacatgca gcatcggaac ccattccggc
     1921 cggtggccca cgaaggtata gaagccggcg agcaccaggg gatagagagg accgacatgc
     1981 gctgccggat tgactccgtc gaatgtaaac tgatgcaggg tcgcgagatt ccacgccaac
     2041 gtatcgaata attgatcggc ggcaaacgca tggccagggg catgcagaat gacccaggtt
     2101 cgggcgaaga gacctgcaac cattgcagag agaaggaaca tccggggttg ccctatgatt
     2161 ggagaatcag gccaattcat ttgatgcaaa ccccgatcaa attggtgtcg tccgtgaccc
     2221 cttcgcgata ctcgatatcg tcgggaacaa gttcatgtaa tgcaggcccg ccttgtgctc
     2281 ggctaatcac gcttccaagc caatgacgaa taggtcgtgg gacacaattg cggaggccaa
     2341 ccggatcaaa attgcgcacg gtgtccatgg agcgttcaac cgccttgaga cgatgcccct
     2401 gacgacgacc gtaccagcgg acagtcgaaa aatgcggttg cagcaaagtc ttaaattcgt
     2461 ccggcgtata ttcacgaacg tggtagcgat cgtcgggaat cactccccga ccctttccgt
     2521 gcggggtaaa tatgacgagc acgccgtttg gtttcagcac ccgggtcaac tcggatacga
     2581 agcagctgtc atcctggaca tgttcgatgg tgtcttgtga gacgatgcag tcgaatgacg
     2641 catctcgaaa ggccagagca caagcatcca ttgcaagaaa cgcgacgttc gatcgaggat
     2701 agtccgcccg gcatttggcg acgacgtcgg gagcgccatc gatccccacg attcgaccgg
     2761 cacaggcacc caaataatcg ctgccatagc cggtaccaca tgaaaggtcc aagacgcacc
     2821 gctctttaag cggtccaagt tggcgggccg cccacacata tcctgataga ttcacgacat
     2881 gctgggtatc tgtcggataa gcggcccttc gctccacgat tccccctacc ccttattcgc
     2941 caggtccata cgaagattca gtacgaatga atgcgcgatc cgttcgacat ttttccccta
     3001 tcccctgttc ggcctggtcc acgcgagccc taatggaccc ggttgccccc agtgccagag
     3061 gcgtcgaaca accgagacat acatgtcgag cgagcagtca ctcacggtcg acgggcggcg
     3121 accgaccgca ccgatcagtc gcacgatcgc accgagcgcg atccacgctt tcatgatcca
     3181 acgcttgaac agcggaaagt gcttttccac gtagcggata cggctgaact cccatacgag
     3241 gttcagctga gtacgatctc gctctccgct ccggccgatc aaatgtacga tgtccgcatc
     3301 ggccaagtgc cgaatgcgat agccccgctg atgaagggct cggcatagat cgacatcttc
     3361 aaaatacatg aagtagcctt catccaatcc gcagcaggcc ctcatcagat ccgtccggac
     3421 catcaagcag gctccattaa tccagtccac atcttg
//
LOCUS       DATNDD010000183         3535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1856752, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000183 DATNDD010000000
VERSION     DATNDD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856752"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VN647_04380"
     CDS             complement(<1..196)
                     /locus_tag="VN647_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96301.1"
                     /translation="MKFLEDPMQTMWAGFALSIVLIIIYLGLTGIGAGEAEWASLILR
                     WVHFLAGITWIGLLYFFNLIN"
     gene            complement(499..1449)
                     /gene="mdh"
                     /locus_tag="VN647_04385"
     CDS             complement(499..1449)
                     /gene="mdh"
                     /locus_tag="VN647_04385"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWG96302.1"
                     /translation="MMTRPKITVVGAGNVGGTTTQRLAEKNLYDVVLVDIAQGVPQGK
                     ALDISQAGPVCGYSTQVVGTNEYEETAGSSIAVITSGIPRKPGMSRDELLATNAKIVK
                     SVVRELVSRSPDITLILVTNPLDAMVHVAHSVSGLPKSRIIGMAGVLDSARMRTFIAA
                     ELNVRATEVQAMVLGGHGDTMVPLSRYTTVKGRPVSELMSKEKLDAIVKRTRDGGAEI
                     VGLLKTGSAFYAPSASAVAMVESIHKDEKQVMPCAVLCEGEYGLKNVVVGVPVKIGRG
                     GAEQILEYELTSDERAALETSAGAVRELCAAVDRLMTLGG"
     gene            complement(1542..1946)
                     /locus_tag="VN647_04390"
     CDS             complement(1542..1946)
                     /locus_tag="VN647_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96303.1"
                     /translation="MTNSIGKPAIPPWLYKLFTGHQYPYVRRLAKFGQTVKPGEDRPE
                     PTKEMIEAKFWEVYPRCWVKVLQEVKEGMIVVFHDLGEYHPGGFQELVDNPEEFLAKT
                     YGKKKIKVNFYDEDNFVCTINFKVAGWTEHEH"
     gene            complement(1959..>3535)
                     /locus_tag="VN647_04395"
     CDS             complement(1959..>3535)
                     /locus_tag="VN647_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWG96304.1"
                     /translation="VRDLWIVLEDARIEFLLQTEYPGLRRDLARFAAESIVPRDPAQG
                     VTPKELIVDGLLRLSTGETEDSAVPMAVREEVSILWSMCRSILKSTATAEETVRVVDQ
                     AYVRLEELLVARDDMMCPERREEPEDAETGQTKPEQPGDQYRAVTDLAYRGSMNPEFI
                     AWSQEQFDRQPEPPAQSDGRAKGNVRIGEQDLGSKDMLDDGRSLPSIVEECLTLEVET
                     PPIRELATHDGRAILYAEWDYRIADYRMNWCRVVERAAEAGPDDFVTETLASHRSTVK
                     SLRRFFEGLRPPAFRRIAGQPDGEEVDLDAVVRRTTEQRAGMEGDDRLYIRREKRERD
                     VAVAFLIDISGSTSRDLGTGRRVIDIEKEGLVLLCEALDAVGDQYGLYAYSGQGRAKI
                     EFLTIKDFDDRLGAATAHRLGGLAPRHQNRDGAAIRHATAKLLTREAKNRVLILLSDG
                     RPLDDQYKDDYSLEDTKAALREARQRGVETFCITIDREAENYLRRMYADAQYCVIDRV
                     EALPMKLPGIYRRLTA"
BASE COUNT          708 a         1123 c          853 g          851 t
ORIGIN      
        1 cattgatcag gttgaagaaa tacaaaagcc cgatccacgt gatcccagcc aggaagtgca
       61 cccagcgaag gattagggaa gcccattcgg cttcacccgc gccaattcca gttaaaccta
      121 aatatatgat aatcaatacg atggacagcg caaagcccgc ccacatcgtc tgcatcggat
      181 cttcaagaaa tttcattgtt ctctctcctc tgaacggttg attgtctcgc aggatgctga
      241 aaaaccccgc cagcggcgtt ctcgcatcgc tcagaggctc aacataccga agcgtacgcc
      301 tcgccccttc gctcgctgcg gtcttgctgg acagcctttg agcatcctga tgttattccg
      361 catctgctag ccggatgtct gtactgtagc ttttctccta ttgtcaaggc gccatgtctg
      421 tcggccggtc agcggtcact tgtcattggc gctcgtcaat gatgagggac tcgttttctt
      481 tctcttacta atgaccactc acccacctaa tgtcatcaac cgatcgaccg ccgcacacag
      541 ttcccgcacc gcgccggccg aggtttccaa cgccgcccgt tcatcgctgg tcagttcata
      601 ctccagaatc tgttccgccc ctccgcgtcc gatcttcaca ggaaccccga caacgacgtt
      661 tttgagtcca tactcccctt cacacagcac cgcacagggc atcacctgct tctcatcctt
      721 gtggatcgat tcaaccatcg ccactgctga agcggacgga gcatagaagg cactgcccgt
      781 tttcaagagg cccacgattt cggctccccc gtcccgcgtt cgtttgacga tagcgtccag
      841 tttctccttc gacataagtt ccgacaccgg cctacccttc acggttgtat agcgcgacag
      901 cggcaccatc gtatcgccgt gcccccccaa caccattgcc tgcacttccg tggcccgtac
      961 gttgagttca gcagcaatga acgtccgcat tcttgccgag tccagtactc cggccatccc
     1021 gatgattcgc gatttaggga gaccgctgac agaatgcgcc acatggacca tggcatccaa
     1081 tggattggtc acgaggatca gagtaatgtc cggagacctg gagactaatt ctctgacgac
     1141 ggatttcacg attttcgcat ttgtcgccaa caactcgtct cgactcatgc cgggctttct
     1201 cggaatgccg gacgtgatca cggcgatcga cgacccggcg gtttcctcgt actcattggt
     1261 gccgaccacc tgcgtgctat acccacagac cggccccgcc tgtgaaatat cgagcgcctt
     1321 gccctgtgga accccttggg ctatatcgac cagtaccacg tcgtatagat tcttctcggc
     1381 taaccgctgc gtcgtcgtcc ctccgacgtt ccccgcacct accactgtga tcttcggtcg
     1441 tgtcatcatg tcctcacatc aagggctgaa gactgagggc cgatgactga gcagtttgag
     1501 ttcaccgtct cagtcctcag catttcgccc tcagtcctga tctaatgttc gtgctccgtc
     1561 cagcccgcaa ctttgaagtt gatcgtgcag acgaaattat cttcgtcata gaaattgacc
     1621 ttgatcttct tcttgccgta tgtctttgcc agaaactcct ccggattgtc gacgagttcc
     1681 tgaaagccgc cgggatgata ctcgcccagg tcatgaaaga ccacgatcat cccttccttg
     1741 acttcctgca gcactttcac ccagcaacga ggatagactt cccagaactt tgcttcaatc
     1801 atctctttcg tcggctccgg acggtcttca ccgggtttca ccgtctgtcc gaacttggct
     1861 aaccggcgga cgtagggata ctgatgtcct gtgaagagct tgtacaacca cggaggaatc
     1921 gctggctttc cgatcgagtt cgtcatgttc ttgagttctt atgcggttaa tcgcctgtaa
     1981 attccgggca gcttcatcgg aagggcttcg acgcggtcta tgacgcagta ttgtgcatcg
     2041 gcgtacatgc ggcgtagata attttcagct tcccgatcga tcgtgataca aaaggtctcg
     2101 actccccgtt gtcttgcctc gcggagcgcc gctttcgtat cttccaacga gtagtcgtcc
     2161 ttgtactggt cgtccaatgg tcttccatcg ctcaacagta tgaggacacg gttcttggct
     2221 tccctcgtca gcagcttcgc cgttgcgtga cgaatagccg caccgtcgcg attctgatga
     2281 cgaggcgcca agccaccaag ccgatgcgcc gtcgccgccc caagtcgatc gtcaaagtct
     2341 ttgatcgtga gaaactctat cttcgctcgt ccttggcccg agtaagcata gagtccatac
     2401 tgatcaccga cagcatccag tgcttcacag agcaatacca acccttcctt ctcaatgtcg
     2461 atcacccgtc gaccggttcc aaggtctcgg cttgttgatc cactgatatc gatgaggaag
     2521 gccaccgcca cgtcgcgctc tcgtttttcc cgccggatgt atagccgatc atcgccttcc
     2581 atgcctgccc gctgttccgt tgttcgcctg acaacggcat cgagatccac ctcttctcca
     2641 tcgggttgcc ccgcgattcg tcggaaagcg ggaggacgca agccttcaaa aaatctgcgc
     2701 agtgatttga cggtactccg gtgggaggcc aacgtctccg tgacgaagtc gtcgggtccg
     2761 gcctccgccg ctcgttccac cacgcgacac caattcatcc ggtaatccgc aatccgatag
     2821 tcccactcgg catagaggat ggctcgcccg tcatgagttg cgagctcccg aatcggtggt
     2881 gtttccacct caagcgtgag gcactcttcc acaatggacg gcaatgaacg cccatcgtcc
     2941 aacatatcct tgcttccgag atcttgttcg ccgatccgga cattcccttt cgctcgaccg
     3001 tccgattgtg ccgggggctc cggttgccgg tcgaattgtt cctgactcca tgcgatgaac
     3061 tccggattca ttgagccacg ataggccaaa tcggtcaccg cgcggtattg gtcacccggt
     3121 tgctccggtt tcgtctgtcc cgtttccgca tcctccggct cctcgcgtcg ctccgggcac
     3181 atcatatcgt cgcgagccac caacagttct tccaagcgaa catatgcctg atcgaccacg
     3241 cgaaccgttt cttctgccgt ggccgtagac ttgagaatgg aacggcacat gctccagagg
     3301 atagagactt cttccctgac ggccatgggc accgccgaat cctccgtttc accggtggag
     3361 agtctcaaaa gaccatcgac gatcagttcc ttgggcgtca ctccttgagc cgggtcgcgc
     3421 ggaacgatgg attcagcagc gaatcgcgca aggtcgcgcc ggagtcccgg atactcggtt
     3481 tgcagcaaga attcgatccg cgcatcttcc agtacgatcc agaggtcccg cacca
//
LOCUS       DATNDD010000184         8126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2485654, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000184 DATNDD010000000
VERSION     DATNDD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485654"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VN647_04400"
     CDS             <1..235
                     /locus_tag="VN647_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96305.1"
                     /translation="VEKITGKEEHQPSWDVAPITLMAETHLLHEQPTCPYDDTPITFV
                     SLPNPRRKARYRLLCYYCGRHTEMNWPPPEAKR"
     gene            complement(272..1033)
                     /locus_tag="VN647_04405"
     CDS             complement(272..1033)
                     /locus_tag="VN647_04405"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG96306.1"
                     /translation="MLDQFFVYHPEPWQDRDWARLSGLPLEDVWFQASDGVRLFGWYV
                     EAAADRPIILWCHGNAGNIINRLDNLKLLYRMGLSIFLFDYRGYGKSQNIRPSEKGFY
                     EDAYGAYDYLTRVRKIRPERIILFGRSLGASVAGELAVQRPASALILESSFPSIEAVA
                     RLHYGGFPAHWFLGAEFRLIDRLPNLSLPKLVIHGDQDDIIPIELGHQVFDAAKPPKE
                     WYVIQGAGHNDTYAMGGGAYFRRLADFIRNALAAY"
     gene            complement(1281..1535)
                     /locus_tag="VN647_04410"
     CDS             complement(1281..1535)
                     /locus_tag="VN647_04410"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWG96307.1"
                     /translation="MDAHEYRYTVFFEPAEEGGHIVTCPALPGMVTEGETLEEARAMA
                     KDAIRAYLESLRQDGLPIPPDKDIRLDPVKGELTVALDAA"
     gene            complement(1618..3552)
                     /locus_tag="VN647_04415"
     CDS             complement(1618..3552)
                     /locus_tag="VN647_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HWG96308.1"
                     /translation="MSGPLFSLYTVLVPLVFIACFFVSIISHAQQPDHEAYLQDLIAK
                     AQQKHLSDQREWHLLLHYRKGLFGGYESEQDDPGFFLSPNGKTDPAAELNATLAKFFS
                     DELVGRSRQPAQCAFIARYHWLKEQLNFDSARLAPLACERFHRWYEDFDVQSISLIFP
                     SAFLNNPASMFGHTLFRVDQKGQTEQTRILAYTINYAADVPPDAGLAYPVRGIFGSYK
                     GYFSTIPYYLKVQQYRDIENRDIWEYRLNLTENQLHRFLMHAWELGNAYFDYFFFKEN
                     CSYHLLALLDYADPDLHLTEEFIGWTVPADTVRLIVSKPGLVSDVSYRPSRSTVIKRK
                     RESLPAGERDLADRITQDPRAVTSPVFTQLALPKQAFVLDLASDYLRYRIETTDSPKP
                     EWKERNRAVLTARSQLRIPSEEFIVRPFARQPELGHKMHRASIGGGWRNDDTFEEATF
                     RGGYHDLLDPEVGYTPDAQIEMASITVRHYNRADQTRVERATLLNLLSLSPIDSVFHA
                     PSWKLNIGMNTIRHNDCRLCSNGFLNGGIGGAKEFRLLKREVLFAFAETEANVSKAYH
                     EMHRVGGGATIGMLADLTERWKVMATGTYLRFPLGDKSDDFRWYVGSRFTLAQNWTVR
                     LEYNHRDRDNDVLFSVQAFF"
     gene            complement(3576..4085)
                     /locus_tag="VN647_04420"
     CDS             complement(3576..4085)
                     /locus_tag="VN647_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HWG96309.1"
                     /translation="MSKKVLVLSIAVLFGAQAGLAMAANPDTGPGCGLGKLAWGEYKG
                     QKEIAPQVLQATTNGTFGSQTFGISFGTSGCTNDGKIMSEQKTTMFASLNFEALTAEM
                     AQGKGEHLASLASLMGVPTEQHPAFFAMTQERYTSLVQAGEASPVALVKSLNDAIAGH
                     PVLAQASVR"
     gene            4356..4874
                     /locus_tag="VN647_04425"
     CDS             4356..4874
                     /locus_tag="VN647_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HWG96310.1"
                     /translation="MSIRTTMQSTVRVMPYRSTILKNAINSVLFDKIKIDERHADDYT
                     PLRRLPPYLGSQTINFYSGGPMKHVSRLSLAVIALVGLGLGGCQIVATPMLGGIYTEV
                     KYGTVATDASAATKEGKACGQSILGWIATGDASITAAKAAGGITTVAVVDHSAKNILG
                     ILGEWCTIVRGS"
     gene            4961..5644
                     /locus_tag="VN647_04430"
     CDS             4961..5644
                     /locus_tag="VN647_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96311.1"
                     /translation="MGLASLARLVIPSTLLLCCLCLRDAAIAGEFGDVELSAYGLGAW
                     PRDVNIFNQETTVPASIQEGFGAGFKVGLFPSALRRTVGLELDSNVHGTAVSFPNVAN
                     GQNHGTGRSDLLIINTTFNLILRYPGERIRPYIGAGIGWSHGTLLNPNIAGRDDKDFD
                     SARAFTHQFLGGIQVLLSPTVHLFGKDRPYSFFLFGEYRHLSSDYHWEGLAIDFRTHY
                     GLVGAGLRF"
     gene            complement(5723..6286)
                     /locus_tag="VN647_04435"
     CDS             complement(5723..6286)
                     /locus_tag="VN647_04435"
                     /inference="COORDINATES: protein motif:HMM:NF023238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HWG96312.1"
                     /translation="MNRFSHRIVVATVLLSLITVSTACYGPFNLTKNVYHWNNNVKGS
                     GEVNNKWMKAIVFFGMFIIPVYEFSLLLDTFIFNSIHFLTGDSPIKSSDLHNDGKREV
                     TLGETTLRWTSFKDGATVIYERDGVVDRRATIVTGATGYRLIDENGSVLSEAEYGVDG
                     SVRLLDSECRVINQWSKEQLQSIAQRQ"
     gene            complement(6567..6857)
                     /locus_tag="VN647_04440"
     CDS             complement(6567..6857)
                     /locus_tag="VN647_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96313.1"
                     /translation="MNRPIVIALFFLTACGGTFAYGSRALIDKLENKYFSVLDEWVQR
                     GGKAQDYQSEVLETCGKLVVASATISEALTLPIIQREEFDFRVNVCAKTTAH"
     gene            complement(7045..7476)
                     /locus_tag="VN647_04445"
     CDS             complement(7045..7476)
                     /locus_tag="VN647_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96314.1"
                     /translation="MGRAMLMLILVSLMLVQNACSVAWALKQPPAKDLEGLGIGTPRQ
                     QLIDRLGIPKLSETDPQGRKQDMFEFQSGMHQASKLRIIPYLAADVFTVCLAELILWP
                     MEVMVLKEATCNAFATYDSSQNVETWRVNKASQSGLEAQGC"
     gene            complement(7616..7762)
                     /locus_tag="VN647_04450"
     CDS             complement(7616..7762)
                     /locus_tag="VN647_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96315.1"
                     /translation="MTDFATLLRLLVKHNIDFIIIGGAAVFLEERKAELTPRVKPLGN
                     HIPL"
BASE COUNT         1970 a         2202 c         2027 g         1927 t
ORIGIN      
        1 cgtcgagaag attaccggca aggaggaaca tcagccttct tgggatgtgg cccccatcac
       61 cttgatggcg gagacgcatc tcctccacga acagccgacg tgcccctacg acgatacccc
      121 cattaccttt gtctccttgc ccaatccccg ccgtaaggct cgataccgcc tcctgtgtta
      181 ctactgcggt cgacatacgg aaatgaactg gccgccgcct gaagcgaaac gataactcga
      241 cgaggccgcc cgatcagatg cgcgccaagc ctcagtatgc cgcaagcgca tttctgatga
      301 aatccgccag ccgacggaag taggcgccgc cgcccatcgc ataggtgtcg ttatggccgg
      361 cgccttggat cacataccat tccttcggcg gtttggccgc atcgaaaact tgatgcccta
      421 gctcaatggg gatgatgtcg tcctggtccc cgtgaataac cagcttcggg agtgaaaggt
      481 tcggtagacg atcgatcaat cgaaattctg ctccgaggaa ccaatgcgcc gggaagccac
      541 cgtaatgcaa tctggctact gcttcaatag aaggaaacga cgattcaagt atgagggctg
      601 aggctggtcg ttgcaccgca agttcgcccg ccacggaggc tccgagtgat cgcccgaaca
      661 ggataattcg ttcaggacgg atttttctga ctcgggtgag gtaatcatac gctccatatg
      721 cgtcttcgta gaatcctttc tcactgggac ggatattctg gctcttccca tatccccgat
      781 agtcgaacag gaaaatcgat agtcccatcc ggtacaggag cttgaggttg tcgagacggt
      841 tgatgatatt gccggcattg ccatgacacc acaggatgat cgggcgatca gccgccgcct
      901 caacatacca accgaacaaa cggacgccat cggaagcctg aaaccacaca tcttctagcg
      961 gcaacccgct caaccttgcc cagtctcggt cttgccaagg ttcgggatga tagacgaaga
     1021 attggtcgag catgatagtt catattcccg tcttatgcgt cagacatgaa gggtcattga
     1081 acttcgaaga tttcagacca tgacggacct ggcatgccgg ttagagaaat gtgagaaatt
     1141 cgtcgacgga aagacgcgaa gagtcggccg atcggggtgt ttgagaatgt aatgaccgcc
     1201 cgatgtatga tgaacgaaaa atccgactct ttgcaacgcg cgaagaacct ctttcggcgt
     1261 gagtgaaggc agccgccgac tcatgcggca tcaagggcga cggtcaattc ccctttcaca
     1321 ggatcaagtc ggatgtcttt gtccgggggg atcggaagcc catcttgtcg taaactttcc
     1381 agataggctc ggatggcgtc tttggccatg gcccgggctt cttccaaggt ctctccctcc
     1441 gtcaccattc ccggcaaggc ggggcaggtg acgatgtgtc ccccctcctc agccggttca
     1501 aaaaaaactg tatagcgata ttcatgggcg tccatggttt cctccattga atgcaagtga
     1561 ttttccgact cagtgtaaaa aagcaacagt tcgaagtcaa atagaggcat gtatcaccta
     1621 gaaaaacgcc tgtacgctga acaagacatc attgtcgcga tcgcgatggt tgtactccag
     1681 tctgaccgtc caattttgag ctagcgtgaa gcgcgacccg acgtaccacc ggaagtcgtc
     1741 cgacttatcc cctaacggaa atctcaagta cgtccctgtc gccatcactt tccatcgttc
     1801 ggtgagatcg gccaacatgc cgattgttgc accgccgccg actcggtgca tctcgtgata
     1861 ggccttactg acgtttgctt cagtttcggc gaaggcgaag aggacttctc gcttgagcaa
     1921 ccgaaattcc ttcgcccctc cgatcccgcc gttgaggaag ccgttgctgc aaagccggca
     1981 atcgttatgc cggatggtgt tcatcccaat gttgagcttc caggatggag catggaagac
     2041 ggaatcgatc ggcgagaggg acagaagatt caacaacgtc gcccgttcta ccctcgtctg
     2101 atccgcccga ttgtagtgcc gtaccgtgat ggaggccatt tcaatttggg catcgggcgt
     2161 atagccgact tccgggtcga gcaaatcgtg atagcctccc cgaaaggtcg cttcttcaaa
     2221 tgtatcgtcg ttacgccagc cacctccgat cgaagctcgg tgcatcttat ggcctagttc
     2281 aggctgtctc gcgaagggtc gcacgatgaa ttcctcggat ggaattcgaa gctgactgcg
     2341 ggcagtcaga accgccctat tacgttcctt ccattccggt ttcggagagt ccgtcgtctc
     2401 aatccggtag cgcagataat ccgaagctaa atcaagcaca aatgcttgtt tggggagagc
     2461 aagttgagta aacaccggtg aggtcactgc gcgaggatct tgcgtgatcc gatcagccag
     2521 atctcgctcc cctgccggca atgattcccg ctttcgcttg atcaccgtgc tgcgggacgg
     2581 tcggtagctg acatcggaca ccagacctgg ttttgagaca atcaatcgaa cggtgtcggc
     2641 cgggacagtc catccaataa actcctccgt caagtgcaaa tcggggtcgg cataatccaa
     2701 gagcgcaaga agatggtaag agcaattctc cttgaagaaa aaataatcga agtaggcatt
     2761 ccccagctcc cacgcatgca tcagaaaccg atgcaactga ttttcggtga gattcaaacg
     2821 atattcccaa atatcccgat tttcgatgtc ccgatactgc tgcaccttga ggtaataggg
     2881 aattgtcgaa aagtatccct tataacttcc gaagatgcct ctcaccggat aagcaagacc
     2941 ggcatcgggc ggtacgtccg ctgcataatt gatggtatag gcaaggatgc gggtctgttc
     3001 ggtctgtcct ttttgatcga cgcgaaacag ggtatggccg aacatagagg ccgggttgtt
     3061 tagaaaggct gatggaaaga tcagagagat cgattggaca tcaaaatcct cataccatcg
     3121 atgaaaccgt tcgcaggcga gaggcgcgag acgggcagaa tcaaagttca gttgttcctt
     3181 gagccagtgg tatcgagcga tgaaggcgca ttgagccggc tgtcgggaac gaccgactag
     3241 ttcgtccgaa aaaaacttgg cgagagtggc attgagctcg gcagccggat ccgtcttccc
     3301 attaggcgac aagaaaaaac cgggatcgtc ctgctcgctc tcataccctc cgaagagccc
     3361 cttccggtag tgcagcaaga gatgccattc gcgctggtca gagaggtgtt tttgctgagc
     3421 tttggcgatc agatcctgaa gataggcctc atgatcgggc tgttgagcat gagagatgat
     3481 tgatacgaag aaacaagcga tgaaaacaag tggaaccagc acggtgtaca gtgagaataa
     3541 gggccctgac atcccgccag ggcccttata acacctcagc gtaccgatgc ttgcgcgagg
     3601 acaggatggc ccgcaatcgc atcattaaga gacttcacca atgcgaccgg agatgcttct
     3661 ccagcttgaa ccagagacgt atatcgctcc tgagtcatgg cgaagaaagc gggatgctgc
     3721 tcagtaggca cacccatcaa cgaagccaac gaagcaaggt gttcgccttt tccttgagcc
     3781 atctccgcag taagcgcctc gaagttcaac gatgcgaaca tggttgtctt ctgctcactc
     3841 ataatcttgc cgtcgttggt gcagccggaa gtcccaaagc tgatcccgaa cgtctggcta
     3901 ccaaaggtac cgttcgtggt cgcctgcagc acttggggag cgatttcctt ctggcccttg
     3961 tactctcccc aggccagctt tcccagacca cagccgggac cggtgtctgg atttgccgcc
     4021 attgccagac ccgcctgagc accgaacagc acggcgatag acaatacaag cactttcttg
     4081 gacataagtc ctcctccgtt agaattagtt aagcacccat gctactgcaa aatcgctact
     4141 gcaaaaacac cgagacgaag tataaccaaa ctcactatcg atgtttataa aaaagttggc
     4201 cactagggaa gtaactagca tccacttctc aattgggtgg attatagaca aatcgtagtg
     4261 aagcgccaac aacattttct ctgccttgga gtttcttact tctttcaacc agcgagagcc
     4321 gaatctaaat ggaccacagc ctccaagcag aacacgtgtc tattcgcacc acaatgcaat
     4381 caaccgttcg cgtcatgcct taccgctcga ccatcctgaa gaatgccata aattccgtcc
     4441 tcttcgataa aattaaaatt gacgaacgtc atgcggacga ctatactccg cttcgacgat
     4501 tgcctccata cttaggcagt caaactatta acttctattc aggaggacct atgaagcacg
     4561 tatcccgact ttccctcgcg gtcattgccc tagtgggcct cggtttgggc ggctgtcaaa
     4621 ttgtcgccac tccgatgttg ggcggaatct acactgaagt gaagtatggc accgtcgcaa
     4681 ccgacgcgtc agcagccacc aaagagggca aggcatgcgg ccagtcgatt ctcggatgga
     4741 tcgccaccgg tgatgcaagc attacagccg ccaaggcggc cggtggcatc accaccgtcg
     4801 ccgtcgtcga ccactctgcc aagaatattc tgggcatctt gggtgaatgg tgcacgattg
     4861 ttcggggtag ctgatcccct ctagcacaag ctatgaaagg gcctccggaa caactgccgg
     4921 atggcccttt catttccctc ctttattccc ttgtggaatc gtgggacttg cttccctcgc
     4981 acgcctggtc attccgtcca ctctactgct ctgctgcctg tgcttacgtg atgcggctat
     5041 agcgggcgaa ttcggcgacg tggaattgag cgcgtatggg ctgggcgcct ggcctcgcga
     5101 tgtgaatatt ttcaaccagg aaacgaccgt tcctgcctca attcaggaag gtttcggtgc
     5161 cgggttcaag gtcgggctct ttccttccgc gctacgtcga acagtgggcc ttgaactgga
     5221 ttccaacgtg cacggcacgg ctgtctcctt tcccaacgtt gccaatgggc agaaccatgg
     5281 aaccggtcgc tcagacctgc tcatcattaa tacgacattc aacctgatcc tacgctatcc
     5341 gggggaaagg atccgcccgt acatcggggc cggcatcggg tggtctcatg gtacgctcct
     5401 caatccaaac attgcggggc gagatgataa agattttgac tcagcccgcg cctttaccca
     5461 tcaatttctc ggtggaatcc aagtccttct cagtcccacg gtccatttat tcggtaaaga
     5521 ccggccctat tctttttttc tgttcggtga gtaccgacat ctttcgtccg actatcattg
     5581 ggagggactg gccattgatt ttcgtaccca ctatggatta gtgggtgcag gactgcgctt
     5641 ttgatccctc gacggccgat tcgacaaact ttgcaacatc cttacaactt ctttaagacc
     5701 accaccttcg ctgggcagcg gactattgcc gctgagcgat ggattgcagt tgctccttgc
     5761 tccattgatt gatcacccgg cattcgctat cgaggagacg aacacttccg tctacaccgt
     5821 actcagcttc cgacaaaaca ctgccgttct catcaatgag gcgatagccg gtcgcaccgg
     5881 tcacgatcgt ggcacgacga tcgacaacac catcgcgttc atagatcacg gttgcaccat
     5941 ccttgaaaga agtccatcgg agagtggtct ccccgagagt gacctctctc ttaccgtcgt
     6001 tatggaggtc agaagatttg atcgggctat ccccggtcaa aaaatgaatc gagttaaaaa
     6061 tgaaggtatc caacagtagc gaaaattcat acaccggtat gataaacatg ccgaaaaaca
     6121 cgattgcctt catccattta ttgttcactt caccgctgcc tttgacgttg ttgttccagt
     6181 gatagacgtt cttcgtgaga ttaaacggac cgtagcaagc ggtgctgacc gttataagac
     6241 tcagcaatac cgttgcgaca acaattcgat ggctgaatct gttcatccgc ttcccctttc
     6301 gtcagaatga ttgcgctact cattcgtggt gcgagccctc atctagcaca atacacaagt
     6361 gagcacaacc tgctctcgac tgacggccat caagagacaa ctcgatcagc tattttctta
     6421 ctgctgcgtt actggggaga catatgcttt gaggaagaaa gctggctggg ttctgattat
     6481 ttgaacatag agagtcactt aacgtaggcc gctacgccgg cagagtcttc ccaatactgg
     6541 gattgcggta tcgtcgcaga gaaaccctaa tgagcagtcg tttttgcaca aacgtttact
     6601 ctgaaatcaa attcctctct ttgaatgatc ggaagcgtca acgcttctga gatagtcgca
     6661 gaagcgacga caagcttgcc acaggtttcc agaacttcgc tttgatagtc ttgagctttg
     6721 ccacctcgct gaacccattc atcaagaact gaaaagtatt tgttctcaag tttgtctata
     6781 agcgctctgc ttccataagc aaaggtgccc ccgcaagccg ttaagaagaa aagggctata
     6841 acaatcggcc tattcattca gacccaacct tagagaaatg aggcccaaac aatcgtgctc
     6901 gatctattag ggcggaacag aaattcagag ggctacaacc atataagttg cagccccttg
     6961 ccttgttgag cttatcaaag gcagaatcat gctggacaag cgcttgctgc tcgccggttg
     7021 aaaagagtct gggtttcgct ctcgttagca cccttgcgct tccaagccag actgactcgc
     7081 cttattgaca cgccaagtct cgacgttttg agatgaatcg tatgttgcga acgcattgca
     7141 ggtagcttcc ttcaggacca ttacttccat aggccagaga ataagttcgg caagacagac
     7201 agtaaaaaca tcagctgcca ggtacgggat aatgcgcaac tttgatgctt gatgcattcc
     7261 tgattgaaat tcgaacatgt cctgcttgcg cccttgcgga tcggtttccg ataattttgg
     7321 gatgcctaac cggtcaatga gctgctgcct cggcgtaccg atgcctaacc cttcaagatc
     7381 ctttgcaggc ggttgcttca gtgcccaggc caccgaacag gcattctgga ccagcatgag
     7441 cgaaaccagt atcaacatga gcatcgctct ccccatggat acatctcctt gtgttgaaga
     7501 ttacaaccga tacatcatct aatgaagagt ccccgtacag gcaatcttga aactagagaa
     7561 gcaatactta ctttttggcc agcaatcttt aggctatcct ttaccaggga agaaatcaga
     7621 gggggatatg gtttcccagc ggcttaactc tcggcgtgag ttcagccttc cgttcttcaa
     7681 gaaacacggc agctcctccg ataatgatga agtcgatatt gtgcttgacg agaagtcgaa
     7741 ggagcgtcgc gaaatccgtc atgaagcact gactgagccg gccttccgca gctcagcagc
     7801 cagccgttgg agctcgcata acttcaggaa tcgttgttcg accgtgagct ttaagttttc
     7861 tctgagcagt gtccggtcga tatctcgttt gtacgcttcg attacaggat cagcttgaag
     7921 ccctgtcgcg ctgctctact cagtcgttct aatagatttt ccaagcatgc tcattctgct
     7981 tgacaaagtc tagcacttcc gtccgcgtgg cttcaatgca gccattgtct tgccatcttc
     8041 gtatcttttc gccaattata ttccgtatta gagagtgtgg ccaacgacgt caatatccgg
     8101 ctgatcgcgc gatccaaaga gcatcg
//
LOCUS       DATNDD010000185         8072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_305658, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000185 DATNDD010000000
VERSION     DATNDD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305658"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(524..2284)
                     /locus_tag="VN647_04455"
     CDS             complement(524..2284)
                     /locus_tag="VN647_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWG96316.1"
                     /translation="MSSLEELPAAQDEKPENGIPGLKHWRYDLVAGLQVALVALPLSL
                     GVALASGAAPITGVISAIIAGLVFPFLGGANITISGPAAGLAPALLAAMLTLGQGDLA
                     AGYPLVLVAICLTGAVQVLLSIYKVGRFAMYFPMSVLQGMLAAIGILIIIKQIPSFLG
                     FLTTQTKSIPEAILIPPEIIGMNPKVFAVGSIALALLFGLDSDRVKNQRWARNIPAPL
                     LVVALGGVAGWMLQFPEAYLIHVPKDVFAQGIHFPNFSEMWQRPELWFALLTTVVTLT
                     LIDGTETLATIAAIDKIDPFHRKSDPNVTLRAMGVSNILSSLAGGLIIIPGGIKSTAN
                     IIAGGRTLWANFYCALFMAFIVWFGTNLINRIPLTVLAALLIFIGWRLCAPRIFLKVF
                     SIGAEQVLVATVCVAVTLYTSDILSGIIAGTVTKILMLCFDVVKAMTFERGSLVGPTE
                     SFSARVLAAFRELFGDPIIRIGDGRTHRKGSLPVMSVAAKNMKHPYKIYLSSLCCMNL
                     LKLDERLKDLPNNRDNFMIILAGHVVDHTAMEYLHQFREQHVKEGHNCVILGTEHFLS
                     HSDHRLAYRVSHTDEALAYG"
     gene            2682..3092
                     /gene="arfB"
                     /locus_tag="VN647_04460"
     CDS             2682..3092
                     /gene="arfB"
                     /locus_tag="VN647_04460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWG96317.1"
                     /translation="MLLISPSVSIPDYEIDLHAVRSQGAGGQNVNKVSTAIHLRFDIR
                     TSSLPQIYKDVLLKLRDHRISSDGVITIKAQQYRTRERNREDALDRLRTLIQRAATPR
                     KKRTVTAPTRSSQHRRIERKKRQGRLKVLRRTID"
     gene            complement(3129..3788)
                     /locus_tag="VN647_04465"
     CDS             complement(3129..3788)
                     /locus_tag="VN647_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96318.1"
                     /translation="MMRMKTLILVILAGGLSFPSMSLSAQQSGDRLAEQALEECNLGR
                     LTRDRATRLAHFQQGQILGERAVAAEEGSADAHFALFCNLGELLRINGESFTSLFGLH
                     RMMKELDRALEINPAHIGALSAKGTLLVRLPGLLGGDSEKGERLLQQVIKQAPKAVNA
                     RLALAKVRCEHGHHQEAVTLAADALAVAKEYQQIEFIPEAEAMLQQARANAVKIKEPR
                     S"
     gene            complement(3857..4648)
                     /locus_tag="VN647_04470"
     CDS             complement(3857..4648)
                     /locus_tag="VN647_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HWG96319.1"
                     /translation="MLLKGKKGLVIGVANKHSIAWAIAQSTAGQGAQLLFNYQNERLK
                     QNVEDLAATLSGAKAFPCDVSNDGEITSLMQQVQKEVGQIDFLVHSLAFAPREELSGQ
                     FVNTTRQGFATALDISAYSLVAVARAALPLMPAGGSIVTLTYLGSERVVPHYNVMGIA
                     KAALEASVRYLAHDLGPLNIRVNAISAGPIKTLAARGVSGITKMVDLHKEFAPLRRPT
                     EQGEVGDAALFLVSQLGRGITGEVIYVDGGFNILGMMASGEQGIS"
     gene            complement(4822..5340)
                     /locus_tag="VN647_04475"
     CDS             complement(4822..5340)
                     /locus_tag="VN647_04475"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWG96320.1"
                     /translation="MRRNIYTGKVVTLNVDTVLLPNGVTVDLETIRHPGAAAVVPIKD
                     DGTVVLIRQFRHAAGGFIYEIPAGKLSPGEEPLQCAARELEEEVGYRASSFELLSSIF
                     TAPGFADEVIHVYKATGLTKGRQQLDRDEVLEIVEMPLHLAITRIQDGTIRDGKTIVG
                     LQAVYIGDAANR"
     gene            complement(5423..6526)
                     /gene="gcvT"
                     /locus_tag="VN647_04480"
     CDS             complement(5423..6526)
                     /gene="gcvT"
                     /locus_tag="VN647_04480"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWG96321.1"
                     /translation="MQHTPLIAQHRAAGAKLVDFAGWEMPIHYTGVVDEYYTVRSKAG
                     LFDVSHMGRIVVTGSGAESFLQRMTTNDIAALRPMQAQYSMVCNEQGGIKDDIFVYRL
                     ANGGQFLLCVNASNRDKIVSWLTEHSRGVSGCQISDRSAEIAQIALQGSAARAIVAVL
                     GLSMLEQLKLRESTTVRVADVECLAARTGYTGEFGYEFYVHGQADTVWNKLLDTGKSF
                     GLKPAGLGARDLLRLEMGYLLYGNDIDERTTPIEAGAEWAVRFEKGEFVGKPALLSQK
                     QAGLSRRFVGVELLEKGVPRHGFKILSSSSPQSPIGEVTSGNLSPLLQKGIGLGYVPP
                     EYAELGTDLFIDIRGKAIPAKVVKTTFYKKSKS"
     gene            complement(6534..6977)
                     /locus_tag="VN647_04485"
     CDS             complement(6534..6977)
                     /locus_tag="VN647_04485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWG96322.1"
                     /translation="MKPLRRAYVCLIVVITFLLGWGNAMAGNEKETSMQEVTTSSGLK
                     YVDQVIGAGDAAVVGKTASVHYTGWLENGKKFDSSVDRGQPFSFPLGAGRVIKGWDEG
                     VQGMKVGGKRKLTIPSDLGYGSRGAGGVIPPNATLIFDVELLGVR"
     gene            complement(7025..>8072)
                     /locus_tag="VN647_04490"
     CDS             complement(7025..>8072)
                     /locus_tag="VN647_04490"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HWG96323.1"
                     /translation="RADLLAHVLTEVLRPRLLIGCSGEGVIAGAEELETAPAITVWAA
                     VLPNVNLHSLRSSFSQTHDQFRLSGWPEPGGVEEASFLLLADPFTVPVQDILGILEER
                     YPGTRAIGGLAGGGQEAGMNRLLFNDQVLDSGLVGVRLSGAVNIRPIISQGCRLIGDR
                     FVVTKAERNLIHELGGAPALERLQAVFESLAEEDRRRAHRALHLGIVIDEHRNRFERG
                     DFLIRNLLGADRTTGAVAIGDVVQVGQTVQFHLRDAESATEDLHSLLATDRTDHRHPP
                     LGALMFSCCGRGRGLFGRPNHDVAATMERLGPIPIAGFFAQGEIGPIGNRNFLHGYTA
                     SLAIFAERDQPNSG"
BASE COUNT         1879 a         2268 c         2105 g         1820 t
ORIGIN      
        1 ccgcggcgag cagttcttca ccggattgaa acgtccgaat ccggtgctgc atgcgagaaa
       61 gtagtttttc cagcgcgtta cgaaacgcag cctggtcgtc caccacaaaa atactcgctt
      121 gcattatggg aaatccccct accgcatgct actgtaagcg agattacgct gcaagatatt
      181 tgccgatgcc cattgctctg gattgagaat tgatgaccac ggtacggaca gacatctttt
      241 tgaccagcct aagaaattat cagccccgat aaggcctcag aaaaacaaac gcaatccgtc
      301 acggacacac ccgtcctcta tatatttacc tcaatgcttc gcttccagtg ggtttacaga
      361 tcggttcggc gatggcgggg gatataggga cagacgacga gcacgaaatt cgacgttggc
      421 agccggtccg aaccagcgac ccaattaatc atccattcgc aatgctcctt atttagcgga
      481 tggccttgat atagattcgc caattgactc gttaagtagg tcttcaccca tacgccaaag
      541 cctcatcagt atgactcact cgataggcca aacggtggtc cgaatgagaa aggaaatgct
      601 cagttccaag aatcacacag ttatggccct ccttcacatg ttgctctcga aactgatgta
      661 aatactccat cgcagtatga tccaccacat gcccggccaa aatgatcatg aagttgtctc
      721 tgttattagg gagatccttg agcctctcat cgagtttcaa caggttcatg cagcagagcg
      781 atgagaggta gatcttatag gggtgtttca tgtttttggc tgcgacgctc atgacaggta
      841 aagatccttt tctgtgagtg cgcccgtctc caattcggat tatgggatct ccgaacaact
      901 ctctaaatgc agccaagacg cgggcggaga aagattcggt gggaccgacg agagaacccc
      961 gctcaaacgt catcgccttt accacatcaa aacaaagcat gagaatcttc gtcacggttc
     1021 cagcgattat tcccgacaaa atatctgagg tgtacagtgt taccgcgaca cagacggtcg
     1081 cgaccaggac ttgctcggcc ccgatcgaaa agaccttcaa gaagattctc ggcgcacaga
     1141 gtctccatcc gatgaatatg agcaatgcgg ccaggacggt gagcgggatt ctgttgatca
     1201 ggttggtccc aaaccataca atgaacgcca tgaatagggc gcagtagaaa ttcgcccata
     1261 gagttcgccc tcctgcgatg atgtttgccg tacttttgat gcctccagga atgattatca
     1321 atccaccggc taagctggag aggatattgg acacgcccat ggctcgaagc gtgacattag
     1381 ggtccgattt tctgtggaag ggatcgatct tgtcgatggc ggcgatggtg gccagcgtct
     1441 cggttccgtc gatcaaagtc agagtgacaa ccgtggtcaa taacgcaaac catagttcgg
     1501 gccgctgcca catctccgag aaattgggga aatggatgcc ctgggcaaaa acatctttag
     1561 ggacatgaat caaataggct tcgggaaact gcagcatcca accggctacc ccgcccaagg
     1621 caacgaccaa gagcggtgca ggaatattcc tcgcccatct ttggttcttg actctgtcgc
     1681 tgtcgaggcc gaaaaggagg gccaacgcga tacttccgac ggcaaagacc ttggggttca
     1741 ttcctatgat ctcagggggt atgaggatgg cttcaggaat cgattttgtc tgagtcgtca
     1801 ggaagcctag gaaggaggga atttgcttga tgatgatcaa tatcccgatg gcagccaaca
     1861 taccctggag caccgacatc ggaaaataca tggcaaaacg accgacctta tagatactca
     1921 gcaacacctg tactgcaccg gtaagacaga tggccacgag caccaggggg tagccggccg
     1981 ccaagtctcc ttggccgagg gtcagcatag cggccagtag ggcgggtgcg agacctgccg
     2041 ccggtccgct gatggtgata ttggctccgc cgagaaacgg aaagacaagt cctgcaatga
     2101 tggccgagat tacccctgtt atgggagcgg ctcccgaggc cagtgcaacc cccagggaaa
     2161 gtggaagtgc caccagcgca acttgcaacc ctgccactag gtcatagcgc caatgtttta
     2221 acccagggat tccattttct ggtttttcgt cttgggcggc aggcaactct tcaagagacg
     2281 acattgcacc ccttcctgtt ctgacagact ggtctttcaa cactcactaa catcgaaggt
     2341 gctgaccaat tgccagtacg gcagccaagt gctcactgaa tcacaaagca catccgatgc
     2401 ccgtgttatg aactgagggg ttcatgcgat agagacgcac aggagaaata ttttcaataa
     2461 tttctgtgag ttgaatgatg tctcctgtga atgaacctta cgcatgatta ttattttaat
     2521 atttgaacga gcgcatttac tatgatctag atgtgcgaag acgctctctt ttgaggatca
     2581 tgttctgttt gttattagac cagactcaac gggaaaattg aacgaaatcc gttatgaggc
     2641 cgctcattgc accgacatat aggctatgct agttattcag catgctgctc atttcgccgt
     2701 ccgtttcgat tcccgattac gaaattgatc ttcatgcggt gcgatcgcag ggggccggtg
     2761 gacagaacgt caacaaggtc tccaccgcca tccatctgcg atttgatatc cgtacgtcat
     2821 ctttgcccca aatctacaaa gatgttctgt tgaagctgcg cgatcatcgc atatcatccg
     2881 acggggttat cacgattaag gcccagcaat accgtacacg ggagcgcaac cgtgaggacg
     2941 cgttggatcg gttacggacg ctcattcaac gggcggcgac cccgcggaag aaacgaacag
     3001 tgaccgcccc gacacggagt tctcagcacc gacggattga gcgaaagaag cgacagggtc
     3061 gtttgaaggt gctgaggcga acgatcgact gacgaggatg ggacggcctt cgtctacgtt
     3121 gaaacaggct aggagcgcgg ttccttgatt tttacggcgt tggctcgtgc ctgctgcagc
     3181 atggcttctg cctccggaat gaactcaatc tgttggtatt ccttagcaac agccaacgca
     3241 tcggcggcta gcgtcacggc ttcctgatgg tgtccgtgct cgcaccgaac cttggcgagt
     3301 gccagccggg cattgacggc tttgggtgcc tgtttgatga cttgttgcaa aagtcgctcg
     3361 cctttttcag aatcacctcc aaggagaccg ggaagcctca ccagcaaggt tcccttggca
     3421 gagagcgcac cgatgtgagc tggattgatt tcgagggctc ggtcgagttc cttcatcatg
     3481 cgatggaggc cgaaaagcga ggtgaaggat tctccattga tgcggagcag ctcccccaga
     3541 ttgcaaaaca gcgcaaaatg ggcatcggcg cttccctcct ccgctgccac cgcacgttcg
     3601 cccaagatct gtccctgttg aaaatgagcg aggcgcgtcg cacgatccct ggtcagtctc
     3661 ccgagattac attcttcgag ggcctgttca gcaagacggt cgcccgactg ctgtgccgaa
     3721 agcgacatgg atgggaagga caggccgcct gccaggataa ctagtatgag ggtcttcatc
     3781 cgcatcatgc aggcatccaa cgggaagacg gcatcatatc gtctggattc aacgctgatc
     3841 ttcgatcgcc gataggtcag gagattccct gttcaccgga agccatcatg cccaaaatat
     3901 tgaatcctcc atccacgtag atcacttcgc cggttatacc tcgtcctaac tgactgacta
     3961 aaaacaacgc cgcatcccca acttcgccct gctcggttgg gcgacggagc ggcgcaaact
     4021 ccttatggag gtcgaccatc ttggtgatgc ctgaaacccc gcgcgcggcc agtgtcttga
     4081 tcggtccggc ggagatcgca ttcactcgga tatttagagg gccgaggtca tgtgccaagt
     4141 agcggactga ggcctcaagt gcggctttgg cgatgcccat tacgttgtag tgaggcacga
     4201 cgcgctcgct gccgagataa gtgagggtga cgatagaacc tccggcaggc attaacggca
     4261 aggcggctct cgcgacggcc acgagagaat aggcactgat gtcaagtgcc gtcgcaaatc
     4321 cttgtcgagt ggtattcacg aattgtccgc tcaactcctc ccggggagcg aatgccaggg
     4381 agtggacgag aaagtcgatt tgaccgactt ccttctgaac ctgctgcatg agcgacgtaa
     4441 tctcaccatc gttgctcaca tcgcagggga atgctttcgc accggataag gtagcggcaa
     4501 ggtcctccac attttgcttc agccgttcgt tctggtaatt gaaaaggagc tgcgctccct
     4561 gaccggccgt ggattgcgcg atggcccagg cgatgctgtg cttattggcc acgccgatga
     4621 cgagtccttt tttacccttt agcaacatca gaacggttct ccttcgttaa agatcaagtc
     4681 ggcattgatg ggaaactcca cccctatttc accgctagat gccgcgccgt ccccaagtcg
     4741 ttcggcagaa tatcttccgt gcatactgtt actccagcat taccggaaga tcgcctacct
     4801 tctctcaatc ggcagagtcg attatcgatt cgcagcatct ccgatgtaaa ccgcttgtaa
     4861 gccgacgatt gtttttccat ctcggatcgt gccgtcttga attcgtgtga tcgccagatg
     4921 cagcggcatt tcgacgatct ccaatacttc gtctcgatcc agttgttgac gccctttcgt
     4981 gagaccggtg gccttgtaga catgaatcac ttcatccgca aaacccggag ccgtaaagat
     5041 actggagagc aattcgaacg aggatgcgcg atagccgact tcttcttcca gctcccgtgc
     5101 ggcacattgc aacggttctt cgccgggaga aagctttccc gctggaattt cataaatgaa
     5161 tccgccggca gcatgccgga actgcctgat caagaccacg gtcccatcat ctttgatcgg
     5221 aacgactgcg gctgcccctg gatgacggat agtttcgaga tcaaccgtca ccccattcgg
     5281 taatagaacg gtgtcgacgt tcagtgtaac gaccttgccg gtatagatat tccttctcat
     5341 gggagcagat gggtgatagc gaatggctta tggtaatgat tcgtaaaacc gggcttgatt
     5401 ttcttgtttc tgctatatgc catcagctct tggatttttt ataaaacgtc gtcttcacga
     5461 ccttcgccgg aatcgctttg cctcgaatgt cgatgaagag gtccgtcccg agttcggcgt
     5521 attccggagg cacgtaccct aatccaatcc ccttctgaag aagaggggaa agattgccgc
     5581 tggtgacttc tccgatggga gactgtggtg aggatgaaga gagaatcttg aagccgtggc
     5641 ggggtacgcc tttctccagc aactcgacac cgacgaatcg gcgggacaga cccgcttgtt
     5701 tttgactcaa cagggcaggc tttccgacaa actccccctt ctcaaaacgc actgcccatt
     5761 ctgctccggc ctcgatcggg gtcgttcgtt catcgatatc gttgccatag agcagataac
     5821 ccatttcgag ccgcaagaga tcccgtgcgc caagacctgc cggtttcaaa ccgaacgact
     5881 tgcctgtgtc caacagctta ttccagactg tgtcggcttg tccgtgcaca taaaactcat
     5941 agccgaattc tccggtatag cccgttcgag ccgccaggca ctcgacatcc gcgactctca
     6001 ccgtcgttga ttctctgagc ttgagttgtt ccagcattga caatccgagc accgcgacaa
     6061 tcgctcgcgc agccgaacct tgcaaggcga tctgcgcgat ctctgctgag cggtcggaga
     6121 tctgacaacc cgaaactccc cgactgtgct cggtaagcca tgatacgatc ttgtctcgat
     6181 tggatgcgtt gacgcacaga agaaattgtc cgccattcgc caaccgataa acgaagatat
     6241 cgtccttgat accgccctgc tcgttacaga ccatcgagta ctgcgcctgc atgggccgca
     6301 gcgcagcgat atcgttcgtc gtcatgcgct gaaggaaaga ttccgcgcct gatccggtca
     6361 cgacaatcct ccccatatgg ctgacatcga acagaccggc cttgcttcga acggtgtaat
     6421 actcatccac aacgccggtg tagtgtatgg gcatttccca ccctgcaaag tcgaccagtt
     6481 tcgccccggc tgcgcgatgt tgtgcgatga gaggggtgtg ctgcaccgga cggttaccgc
     6541 acaccgagca attcgacgtc gaaaatcagc gtggcgttcg gtgggatcac tccgccggca
     6601 ccacgcgatc cgtatcccaa gtcggatggg atggtaagtt tgcgcttgcc ccccaccttc
     6661 atcccctgca ccccttcgtc ccagccctta atgacccgcc cggcaccgag gggaaacgag
     6721 aagggctgtc cacgatcgac ggaactatcg aacttttttc cgttctccag ccaacccgta
     6781 tagtgtacgc tcgccgtctt tccgacgacc gccgcatctc cggcgccgat cacctgatcg
     6841 acgtatttga gcccagacga tgtggtgact tcctgcatgc ttgtctcctt ctcattcccg
     6901 gccatggcat ttccccatcc tagtaaaaac gtgatgacca cgatcagaca gacataggca
     6961 cgacgcagag gtttcataaa ctaccctcct tcgctcactg tatggcatga gagagactac
     7021 tcggtcaacc ggagtttggt tggtctctct cggcaaagat cgccagacta gccgtgtatc
     7081 catgcaagaa gttacggttt ccgatgggcc ctatctcgcc ttgcgcaaag aagcctgcga
     7141 tgggaatcgg tcccaggcgt tccatagtcg cagcgacgtc atggttcggt cgtccaaaaa
     7201 gccctcgacc acggccgcaa cagctgaaca tgagtgcgcc gagcggagga tgccggtgat
     7261 cggttcgatc ggtcgccaac agagagtgca aatcttccgt ggctgattcc gcgtcgcgca
     7321 gatggaactg caccgtctgg cctacttgca ccacatcgcc gatcgcaacc gcacccgtcg
     7381 tacggtcggc tccgagcaga ttccgaatga gaaaatctcc tcgctcgaac cgattccgat
     7441 gttcatcgat cacgataccg agatggagcg cccgatgggc gcggcgtcgg tcttcctccg
     7501 ccaacgattc aaagacggcc tgtaaccgct ccaaagccgg cgcgcccccg agttcgtgaa
     7561 tcagattgcg ttccgctttc gtcacgacaa accggtcgcc gatcagacga cacccctgtg
     7621 agatgatcgg tcgaatattc acggctcccg atagacggac gcctactaac ccgctatcga
     7681 ggacttgatc gttgaagagc aaccgattca tgccggcctc ttggccacct ccggccaatc
     7741 cgccgattgc cctcgttcca ggatagcgct cttccaaaat cccaagaata tcttgaactg
     7801 ggacggtgaa tggatctgcg agcaggagga acgacgcctc ttcaactccc cccggctcag
     7861 gccatcccga gagacgaaat tgatcgtgcg tctgggaaaa cgacgatcgg agtgagtgca
     7921 gattaacgtt gggaagtacg gcggcccaga cggtgatcgc cggagccgtt tccaattcct
     7981 ctgctccggc aatcaccccc tctccgctgc agccgatcag tagcctcgga cgaagaacct
     8041 ctgtcaacac atgagccaag aggtcggccc tg
//
LOCUS       DATNDD010000186         2746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1233852, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000186 DATNDD010000000
VERSION     DATNDD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233852"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VN647_04495"
     CDS             complement(<1..309)
                     /locus_tag="VN647_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HWG96324.1"
                     /translation="MKVLCIGDIMGEPGRRAVARAVPRLIAQHRIDAVIANGENLAGG
                     FGVTPELAEELFETGISVITTGNHAWDKKEAVDYFSREPRLLRPANYPAGVPGNGSVV
                     I"
     gene            complement(306..1886)
                     /gene="rny"
                     /locus_tag="VN647_04500"
     CDS             complement(306..1886)
                     /gene="rny"
                     /locus_tag="VN647_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWG96325.1"
                     /translation="MTPISTANVIYIILSVIFGAVVSAGVFEVLRRRMTGAKQAEAEG
                     LAKQVVQNAQREAENILKEARFEAKDLVFKAKSEFEQEQKAKLGELSAMEKRLIQREG
                     GLDGKLVALEKRENESRKHEADFAKREEGLAAKESACAKAEREHRDALERVAGMTADE
                     AKKQLILEMESQAKLDAVGIAKRTIEEARENAEREAREIITTSIQRVVRDYVSESTIS
                     VVPIPNDAMKGRIIGREGRNIRALEAATGIDLIIDETPEAVIISGFDPLRREIAKVSL
                     ERLMHDGRIHPTRIEEIVEKVKVDIDKLMYEEAEKIIFELGLSDFHPELIKVLGRLKY
                     RTSYGQNNLYHAREASYICGIMASELGLDVRLARRGALLHDIGKAVSHEEEGPHAMLG
                     AEIAKKYGESPKIVNAIAGHHEQVEPICPESVLVAAAEALSAARPGARREALESYVKR
                     LEKLESLATGHKGVQKAYAIQAGREIRVIVRQEDITDAESFQLSRELAKKIEQELTYP
                     GQIKVTVIRESRYVEYAK"
     gene            complement(2437..>2746)
                     /locus_tag="VN647_04505"
     CDS             complement(2437..>2746)
                     /locus_tag="VN647_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWG96326.1"
                     /translation="RRWPLTKTIDVEIYGQRYAITGDGDDAYIRRLAHFVDDHMKHLA
                     EGMKTTTPSKLAVLTAINLAHQLFESEKKRVQGEADVERRMVTLMESIDEQMPTSLFR
                     "
BASE COUNT          574 a          856 c          640 g          676 t
ORIGIN      
        1 aatgacgaca ctcccgtttc ccgggacccc cgccggataa ttcgccggac gcaacaagcg
       61 gggctcccga gaaaaataat cgactgcttc tttcttatcc caggcatggt ttccggtcgt
      121 gatgaccgat atgcccgttt caaacagttc ttcggccagc tccggcgtga ccccgaagcc
      181 tccggccaga ttttcaccgt tggcgatgac cgcatcgata cggtgctgtg cgatcagccg
      241 aggcaccgcc cgagcgactg cccggcggcc cggctctccc atgatatccc cgatacataa
      301 gactttcatt tggcgtattc cacgtatcgg ctttctcgaa tgacagtgac cttgatctgt
      361 cccggatacg tcaactcctg ttcgatcttc ttggcaagtt cacgagaaag ttggaacgac
      421 tcggcatcgg tgatgtcctc ttgcctcaca atgacgcgaa tttcacgccc ggcttggatc
      481 gcgtatgctt tctgcactcc cttatgcccg gttgccagcg attccagctt ttccaaccgc
      541 ttcacatagg actccagagc ctcccgtcgc gcaccaggcc gtgccgccga caacgcctcg
      601 gcagccgcca ccaggacact ctccggacag atcggttcca cctgctcgtg atgcccggcg
      661 atcgcattga cgatcttggg agattcgccg tattttttgg cgatttccgc ccccagcatg
      721 gcatgcggcc cctcttcttc atggctgacc gccttgccga tgtcatggag caaggctcct
      781 cgccgagcca gtctgacatc gagacccaat tccgacgcca tgatgccgca gatatacgat
      841 gcttcgcggg catgatagag gttattttgc ccgtagcttg ttcgatactt gagacgcccc
      901 agcactttga tcaactcagg atgaaaatct gagagcccca gctcaaaaat aattttctcc
      961 gcctcttcat acatcagctt gtcgatgtcg accttgacct tttcgacaat ctcctcgatg
     1021 cgcgtgggat ggatgcgccc atcgtgcatc agccgttcta atgacacttt ggcaatttcg
     1081 cgccgcaacg gatcaaatcc ggagataatc accgcttcag gggtttcatc gatgatcaga
     1141 tctatgcccg tcgccgcttc aagcgcccgg atattccgcc cttcccggcc gatgattcgg
     1201 cccttcatgg catcgtttgg aatgggaact accgaaattg tggattccga cacataatca
     1261 cgaacgacgc gttgaattga ggtggtaata atctcccgag cttcccgctc cgcattttca
     1321 cgcgcctctt cgatcgttcg cttcgcaatc ccgacggcat cgagtttcgc ctgcgattcc
     1381 atttcgagaa tcaattgttt cttggcttca tccgccgtca taccggcaac ccgttccaac
     1441 gcgtcgcggt gttcgcgttc ggccttggcg caggcagact ccttggctgc aagcccctct
     1501 tctcgcttcg caaaatccgc ctcgtgcttg cgtgactcat tttcccgttt ttccaaggcg
     1561 acgagctttc cgtccaaccc cccttctcgt tggataaggc gcttctccat cgccgagagt
     1621 tcaccaagct tggctttctg ctcttgttcg aattcggact ttgctttgaa taccaaatcc
     1681 ttggcttcaa acctggcttc cttgagaata ttctcggctt ctcgttgtgc gttctgcaca
     1741 acttgcttgg ccaagccttc cgcctcagcc tgtttggcac ccgtcatccg tcgtcgcaga
     1801 acttcaaata cccctgcact aaccacggcg ccgaagatca ccgacagaat aatgtaaata
     1861 acatttgcgg tagaaatggg agtcaccccc ttcttactta aaaacagtca gatcagtgaa
     1921 gctctatctg gcatcggcac cccgccacag ttcgagtgga acgggcgcta accacgatgt
     1981 gaggtgaaga aatggaaggg cctttaggag accgcgagcc gtctcgcctg gaaacgcaag
     2041 agatcgtcgg gatacgatac cacgtccggt aagtttctct gtctgccctg cttcgatggt
     2101 cggtcgcatg acaccggctg tttgagaagg gcagctatcg aaacagggat ggcccgcaga
     2161 tactcgttcc gtccggcagg catctgccga atcgcttcaa cttcaaaaat tggacgttgc
     2221 ctggccgact gaagtgcccg cctaagcacg acccgtacca tgagtatcca ccaaaccaac
     2281 gcttcgtatc ccatgaggta gacgttctct gtagtgctca aactcgtttc cagtagatcg
     2341 acaatcgacg acaccaccct tacttctatc accgcacaac cacttatcta tctatatgat
     2401 aacaaaggga ctcttcgaaa gcaaggcgaa ttaccgctac cgaaagagcg atgtcggcat
     2461 ctgttcgtcg atcgattcca tcaacgtcac catccgccgt tcgacatcag cctccccttg
     2521 gactcgtttt ttctccgact caaacagctg atgggcaagg ttgatcgccg tcagcaccgc
     2581 caacttcgac ggagtcgtcg tcttcatccc ctccgccaga tgtttcatgt gatcgtctac
     2641 gaaatgggcc agccgccgga tataggcatc atcgccgtca cctgtgatcg cgtaccgttg
     2701 accataaatt tctacatcaa tggtcttagt caatggccac ctcctt
//
LOCUS       DATNDD010000187         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2885716, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000187 DATNDD010000000
VERSION     DATNDD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885716"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..827)
                     /locus_tag="VN647_04510"
     CDS             complement(36..827)
                     /locus_tag="VN647_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWG96327.1"
                     /translation="MQVGFYQFNPQFGEVAKNLDVVAAKLEQVEADLLVLPELFASGY
                     QFLSREEVQQFAEPVPDGQTVRRLIDIAKHRKMHLVAGLPEESGGRCYNSAVVVGPSG
                     FLGCYRKTHLFYEETQCFTSGDSGFLVWDIGSAKIGIMICFDWYYPEAARTLAVQGAD
                     IICHPSNLVLPNCPDSMPVRCLENRVFAVTCNRIGSEARGGKERLTYIGQSEVVTPKG
                     IIQHRAPCDQEELTVVEIDPAQARNKSLNRYNDLLRDRRTSLYKM"
     gene            complement(921..1265)
                     /gene="smbP"
                     /locus_tag="VN647_04515"
     CDS             complement(921..1265)
                     /gene="smbP"
                     /locus_tag="VN647_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HWG96328.1"
                     /translation="MTTTMRRSALIMAAIGALVGMPMLSGMAFAADKHAAEAVEHAKA
                     AVSHGKEGHADACVEHASEALKHAQATVKNPHGAEGVKHLTEAVKHGKAGHADACTEH
                     AEGAATHLAEVK"
     gene            complement(1349..2227)
                     /locus_tag="VN647_04520"
     CDS             complement(1349..2227)
                     /locus_tag="VN647_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWG96329.1"
                     /translation="MKHPLLIDTETLQRHLGRPDLVVIDVRGKSAYEFGGHIPGAVHS
                     TWHEYSDPNAVPKGLLNPDLGQIERILSRLGMNQGSDVVIYSNPFDNWGDEGRMFWML
                     EYLGHKRLRILDGGWVKWTAEKRPFEHGSVSPMMGNFKAQPVQALIALKDDLKGLVRG
                     PHPQTVILDARSVEEYLGKEVSGLPRSGHIPSAIHMAWNGFLNKDATVKDLSVIKEML
                     EVKGVRNGQEVICYCTGGVRSAWLYFILKLVEYPKISNYPGSWWEWSRDFACPAEKDV
                     HALQKILEFDPAAKPS"
     gene            complement(2265..>2615)
                     /locus_tag="VN647_04525"
     CDS             complement(2265..>2615)
                     /locus_tag="VN647_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWG96330.1"
                     /translation="TERGKGEGVGFTVNVPMEEGEGDEAYHAIFLKSLVPAADAFKPE
                     FVIISAGFDAHKDDPLAGMGLTEAGYADLTEIVAGIAKRHAKGRILSSLEGGYNLTAL
                     AHSVDAHINALFNA"
BASE COUNT          571 a          796 c          654 g          594 t
ORIGIN      
        1 accgggcttc tctcaacagg gtgtgaaact cggggttaca tcttgtacag cgacgtgcgg
       61 cggtcgcgaa gcagatcgtt gtagcgattc aagcttttgt tgcgcgcttg cgccggatca
      121 atctcaacga cggtcagctc ttcttggtca caaggcgccc gatgttgaat gatccctttg
      181 ggtgtcacca cttcgctctg gccgatatag gtcagccgct ctttgccacc ccgcgcttcg
      241 ctgccgatac gattgcacgt cacggcgaac acccgatttt caagacaccg gaccggcatg
      301 gaatccgggc agttcggcaa gaccagattg gatggatggc aaatgatgtc cgccccttgt
      361 accgctaagg tccgcgccgc ctccggatag taccaatcga aacaaatcat gatgccgatc
      421 ttcgccgacc cgatatccca cacgagaaac cctgagtcac cggaagtgaa acattgggtc
      481 tcttcgtaaa agagatgggt cttccgatag cacccaagaa aacccgacgg ccccacgacc
      541 acggcggaat tgtaacagcg gcccccggac tcttcgggga gtcctgccac aagatgcatc
      601 ttgcggtgtt tcgcgatgtc gatcagacga cgtaccgtct ggccgtcggg aaccggctca
      661 gcgaactgtt gcacttcctc ccgtgaaaga aactgatagc cggaagcgaa caactccggc
      721 agcacgagga gatccgcttc tacctgttcc aacttggccg cgacgacatc gagattcttc
      781 gccacttcgc cgaactgtgg attgaactgg taaaacccga cttgcacggc acactccgtt
      841 caaataaaag cgggcaggga ttgtgtccct gcccgcttca acaaaggccc cttgcagcag
      901 ccgcggctgc ttctcgtcaa ctacttcacc tcagccagat gcgtggccgc cccttcggca
      961 tgttcggtac aggcatcggc atgcccggct ttaccatgtt tcacggcttc cgttaagtgt
     1021 ttcacacctt ccgccccatg tgggttcttt actgtcgcct gagcatgctt gagtgcttca
     1081 ctcgcatgct caacacaagc atcggcatgg ccctcttttc catgcgacac agcggctttc
     1141 gcgtgttcaa cggcttcagc cgcatgcttg tcggcggcaa atgccattcc gctcagcatc
     1201 ggcatgccga cgagcgctcc aattgcagcc attatcaaag cacttcgacg catcgtggtc
     1261 gtcatcgttg cctcctccgt ggttgaaatt cgctgctcaa gagaacaatc gaccgtgaat
     1321 tcacctttac acagcgctac tcaggctgtc aagaaggttt ggcggctggg tcaaactcga
     1381 gaattttctg gagcgcatga acatcctttt cggccggaca ggcgaaatcc cgactccatt
     1441 cccaccatga tccgggataa ttgctgatct tcgggtactc gaccagcttg agaatgaaat
     1501 agagccaagc cgatcgcaca ccgccggtac agtagcaaat aacttcctgc ccgttccgta
     1561 ccccctttac ctccaacatt tccttgatca cggagagatc cttgacggtc gcatccttgt
     1621 tcaagaagcc attccacgcc atgtgaatgg cggaggggat gtgaccggac cgtggaagac
     1681 cggacacctc tttcccaaga tattcttcaa cactgcgcgc atccagaatg accgtctggg
     1741 gatgcggccc cctgacgagg cctttcagat cgtccttcaa cgcgatcaga gcttgcaccg
     1801 gttgggcctt gaaatttccc atcatcggag agaccgaccc atgttcgaat ggccgcttct
     1861 cggccgtcca tttcacccac ccaccatcca aaatacgcag acgcttgtgg cccagatatt
     1921 ccagcatcca aaacatccgt ccctcatcac cccaattgtc gaatggattg gagtagatca
     1981 cgacgtcgct gccttgattc atgccgagcc gactcaagat acgttctatc tgaccgaggt
     2041 cgggattgag caatcccttg ggcacggcgt tgggatcact gtactcatgc caagtcgaat
     2101 gcacggctcc ggggatatgt ccgccgaatt cgtacgccga cttgccgcgg acatcgatga
     2161 cgacaagatc aggacggcct aagtgccgct gaagcgtttc ggtatcaatt aataacggat
     2221 gcttcatgaa tatcttcttt cgttacagtt cgcggtagcg tgattcaagc attgaataac
     2281 gcgttgatgt gcgcatccac tgagtgagcc aacgccgtca gattgtaccc accttcgaga
     2341 gaagatagga tgcggccttt cgcatggcgc ttggcgatac cggccacgat ttccgtcaga
     2401 tcggcatatc ctgcctctgt caaacccatg ccggccaacg ggtcatcttt gtgcgcatcg
     2461 aatcccgcag aaataatgac gaactccggc ttgaacgcgt cggccgctgg gacaagcgat
     2521 ttgaggaaga tcgcgtggta tgcctcatcg ccttccccct cttccatcgg gacattgact
     2581 gtaaatccta ctccctctcc cttgcctcgt tcggt
//
LOCUS       DATNDD010000188         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2488975, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000188 DATNDD010000000
VERSION     DATNDD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2488975"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="VN647_04530"
     CDS             <1..1835
                     /locus_tag="VN647_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HWG96331.1"
                     /translation="ITFSAAIIRQNMEKLRLSIIQRHIVSSARWTAFLLSFALASLCP
                     AQMGVSTGNGVAVPAKPLPAGVKPPVVRYEDIAAQAGLTGVNVSGAEKNKQYIIETTG
                     NGVAIFDYDNDGLPDILFVNGDRLHGGGTSTPFLYHNLGGLKFEDVTAKGGLTHTGWG
                     QGVCAGDIDDDGHVDVFITQWGQNVLYHNQGNGTFKNETEARGLKSPKARWSTGCAFV
                     DFNRDGALDLVVAHYIEFDPATTPHPGEKSQCEWKGLPVICGPRGLQGETISLYQNDG
                     HGHFTDVSDQMHITTPKNYYCFTPLVADFDNDGWPDIYVACDSTASLYFHNLKGKGFE
                     EIGVESGVAYNDEGRAQAGMGAAAADFTHSGRLDIFKTNFADDTSTLYSNQGANSFND
                     ATIDAGLAANTRYLGWGTAAIDIDNDGWKDLVLANGHVYPEVDSGHTGETFKQSRLLY
                     WNRRDGQFFDLSPMAGSGITATHASRGLAVGDLNNDGNEEVVIVNMGETPSLLKNVAP
                     PLGNSILIRALTATGRDAIGARIHVTANERTQIDEVRSGGSYVSQNDFRLHFGLGKAT
                     IANLSVRWPDGKVENFSSVAAGQIVTIQEGKGIVGKVPYTSRKP"
     gene            complement(1857..3440)
                     /locus_tag="VN647_04535"
     CDS             complement(1857..3440)
                     /locus_tag="VN647_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96332.1"
                     /translation="MSPITPAFLNGISSSAIYVNPRLNRRFEVFSKEGKLYQSESGLD
                     ADGKESFRDAHQLEWIIGAGVNGFGAILQDDHYLFQAPLSFYSKAKDWEPSPGYEFAD
                     LGFNRPITPGCISCHSGRPNAVAERNGKFERVPFSELPIGCERCHGPGAAHVQLMSGG
                     TVSGSGKDGSSTTMIVNPARLTPYLADNICMACHQAGDVRVLKPGKTYRDIRPGLPLD
                     ETLSILMVPPTPDTPPAKDHVDHYYSMTLSKCYRASKGQLSCISCHDPHVEPSQKEAP
                     AYFNSKCLTCHTRQSCKLPLKTRMQQKPADNCIACHMPKRDIQVISHSSATNHRIVAT
                     PDEPFPDVTFMQTTAALPNLIHLNPASRSASDHQADPLPLLTLLQAYGELAQNKPEYV
                     ASYLKVLSQLEETQPNDPLVQAALGRKDLKDGNFSSAVTHLRRSLEAGPATATTYADL
                     ADALTHLGQTVEARPLIEKAIELDPFNPITRKMLVVNHIEAKQYPEAQKSLEDYLKVF
                     PQDSFMRQMLNRAEGTSHP"
     gene            complement(3716..>4315)
                     /locus_tag="VN647_04540"
     CDS             complement(3716..>4315)
                     /locus_tag="VN647_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWG96333.1"
                     /translation="YVLIDRDLDGVDANFVGINDKRAGYLATEHLVSMGCRRIAHIRG
                     QDNSPGNGRFQGYKDALLDNNLLFSDNYVVRRSYVDIETTRQGAEAMRLLLELDPKPD
                     GVFCFNDPLAIGAMSTILEAGLRIPEDIALIGCGNLPNNDWLRVPLSSIDQRSQTVGR
                     RAAELALNLIESKHMPRTQTTVLEPTLVIRSSTRKREIK"
BASE COUNT         1002 a         1083 c         1227 g         1003 t
ORIGIN      
        1 agatcacatt ttccgctgct ataattcgcc agaacatgga gaagctccgt ctcagcatta
       61 ttcaaaggca catcgtttca tcggctcggt ggacggcgtt tcttcttagc ttcgctttgg
      121 ccagcttgtg ccccgcccag atgggagtat caaccggaaa cggcgtggct gtccctgcga
      181 agccgttgcc tgctggagta aagccgccgg tagtgcgtta cgaagatatt gcggctcagg
      241 ctgggcttac cggagtaaat gtctccgggg cggaaaaaaa taaacaatac atcatcgaga
      301 ccaccggaaa tggagtcgca atcttcgatt acgacaacga tggtttaccg gacattctgt
      361 tcgtcaacgg cgatcgtctc cacggtggag gcacctctac accatttctg tatcacaacc
      421 tgggcggcct gaagtttgaa gatgttactg ccaaaggggg attaacgcat accgggtggg
      481 gacagggcgt ctgcgccggg gatatcgacg atgacggaca tgttgatgtc ttcatcacac
      541 aatgggggca gaacgttctt taccataacc agggcaatgg cacgtttaag aacgaaacag
      601 aggcgcgtgg tctgaaatcg ccaaaggcgc gttggagtac aggatgcgcc tttgtggact
      661 tcaaccggga tggagcgctg gatctggtcg tagctcacta cattgagttt gatccggcca
      721 cgacgccaca tccaggcgag aagtcgcagt gcgaatggaa gggactgccg gtgatctgcg
      781 gtccacgagg actgcaaggc gagacgatct cactgtatca aaatgatgga catgggcact
      841 tcaccgatgt ctcggaccag atgcacatca cgacgcccaa gaactactat tgttttaccc
      901 ctctggttgc agatttcgat aatgacggct ggccggatat ttatgtcgcc tgcgattcca
      961 cagcgagcct ctactttcac aatctgaagg gaaagggctt cgaggaaata ggcgtggaat
     1021 ccggcgttgc ctataacgat gaaggacgtg cgcaggcagg catgggcgct gcagcagccg
     1081 acttcacgca tagcggccgc ctcgatatct tcaaaacgaa ctttgcagac gacacctcaa
     1141 cgctttacag caatcagggt gcgaacagct tcaacgacgc cactatcgat gccggccttg
     1201 cggccaacac gcgatatctg ggttggggta ctgccgcgat cgacattgac aatgatgggt
     1261 ggaaagatct ggttctcgcc aacgggcatg tgtaccctga agtcgacagc ggacatacag
     1321 gcgagacgtt caagcagagc cggttactct actggaatcg cagagacggc cagttctttg
     1381 acctttctcc catggccgga agcggtatta cagcgactca tgcttcccgt ggactggcgg
     1441 tcggcgatct gaacaacgac ggcaacgaag aagtagtcat cgtcaatatg ggagagactc
     1501 cgtcgcttct aaaaaatgta gcgccgcctc tggggaactc catactgata cgcgctctga
     1561 ctgcgaccgg tcgcgatgca attggtgccc gcatccatgt cacagccaat gaacgaacgc
     1621 aaatcgatga ggttcgcagt gggggatcgt atgtatcgca aaatgatttt cgactccact
     1681 ttggcttggg gaaagcgact atcgctaatc tctccgttcg ttggccggat ggcaaggtcg
     1741 agaacttctc ttcggttgcc gcaggccaaa tagtcactat ccaggaaggc aaagggattg
     1801 ttgggaaggt tccttacact tcccggaaac cttaagtatt gcccctcaag atcatttcaa
     1861 ggatggctcg tgccttcggc tcggttcaac atttgacgca taaagctatc ttgcggaaag
     1921 actttcagat agtcctcgag cgacttttgc gcctccgggt attgtttggc ttcgatgtgg
     1981 ttgactacca acatctttct tgtaatggga ttaaacggat cgagctcgat tgctttttcg
     2041 atcaatggcc gagcttcgac tgtctgtcca agatgggtca gagcgtctgc cagatcggcg
     2101 taggtcgtcg cggtagcggg accagcctca agcgaacgcc gtagatgggt caccgcgctt
     2161 gagaagttgc cgtctttcag atctttgcgt cccagcgctg cttgcactaa gggatcattc
     2221 ggctgcgtct cctcaagttg gctgaggact ttcagatagg aggcgacgta ttccggtttg
     2281 ttctgtgcaa gctcgccgta cgcctgtaag agggtcagca gcggaagtgg atcggcctga
     2341 tgatctgacg cagagcgact agcaggattg agatggatca aattcggcag cgcagccgtc
     2401 gtctgcatga aggtgacgtc tggaaatggc tcatcaggag ttgccacgat gcgatgattc
     2461 gtggcgctag agtgtgagat gacctggatg tcgcgtttcg gcatatgaca ggcgatgcag
     2521 ttgtcagccg gcttttgttg cattcgggtc tttaaaggaa gcttgcagct ttggcgcgta
     2581 tggcaggtca ggcatttgct gttgaagtat gccggagcct ctttctgtga cggttctacg
     2641 tgaggatcgt ggcagctaat gcagctgagt tgccccttgc tcgcgcgata gcacttgctc
     2701 aaggtcatcg agtagtaatg gtcaacgtga tccttagcgg gtggggtatc tggtgtgggt
     2761 ggaaccatca ggatagataa cgtctcgtcg agtggaagac ctgggcgaat atcccggtat
     2821 gtttttccgg gcttgagaac ccgaacatcg ccggcctgat ggcaggccat acagatattg
     2881 tccgccagat acggcgtgag tcgggcggga ttgacgatca tggtggtgga cgaaccatcc
     2941 ttccccgagc cagagacggt tcctccactc ataagctgta catgggccgc tccaggtccg
     3001 tggcatcttt cgcagccaat gggcagttcc gaaaaaggta ccctttcgaa ttttccattc
     3061 ctctcggcca cagcattggg gcggccgctg tgacaggaga tgcatccggg agtaatcgga
     3121 cgattaaatc ctagatcggc aaattcgtat ccgggcgaag gctcccagtc cttagccttg
     3181 gaatagaagg ataacggtgc ctgaaagaga tagtgatcgt cctgaaggat tgctccaaaa
     3241 ccattaacgc cagctccgat gatccattcc agttgatgag cgtcccggaa gctttccttg
     3301 ccgtcggcgt caaggccgga ctcgctttga taaagcttcc cttccttcga aaacacttca
     3361 aatcggcgat tcaaccgcgg gttgacataa atggcggaag aagagatgcc gtttaggaat
     3421 gcaggagtta tcggcgacat ggaacggccc atatcggttt tcaagaactt ttgatagatc
     3481 tcgaaatggc aagaggagca ggcgctggag ccgacgtatt ctccattgga tttgtggatc
     3541 ggcggcgggg agagattctt aggcgccttc ccataagcca caaacgccga gagcatcgga
     3601 atgaaaaaaa ggatcgtgac aagtcgggca caacggaagg cagtgctggc ggacccaata
     3661 gatctctcga ttccttgcac tttagaacct gacctggctt tgcagacggg tgacctcact
     3721 taatttccct tttccgagta gatgaccgta ttacgagagt tggctcgagc acggtggttt
     3781 gggtacgagg catatgcttg gactcgatta aattgagcgc aagttccgca gcccttcgac
     3841 ccaccgtctg actacgctga tcaatgcttg agagaggaac tcgaagccag tcgttattgg
     3901 ggagattgcc acaaccgatc aatgcaatgt cttccgggat cctcaggccc gcttcgagga
     3961 tcgtgctcat ggcaccaatc gcgagcggat cgttgaaaca gaagacgccg tcaggcttcg
     4021 ggtctagttc cagcagcaga cgcatagcct ccgcaccctg gcgggttgtt tcgatatcta
     4081 cataactcct acggacgacg taattatctg aaaagagaag attgttatcg agtagggcat
     4141 ccttgtagcc ttgaaatcga ccgttgcccg gactgttgtc ctgcccgcgg atatgggcga
     4201 tgcggcgaca acccatgctt acaagatgct ctgtcgcgag atatccagct ctcttgtcat
     4261 tgatgccaac aaagttagcg tcgacaccgt ccaagtcgcg atcgatgagg acata
//
LOCUS       DATNDD010000189         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3059093, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000189 DATNDD010000000
VERSION     DATNDD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3059093"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(782..1159)
                     /locus_tag="VN647_04545"
     CDS             complement(782..1159)
                     /locus_tag="VN647_04545"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HWG96334.1"
                     /translation="MTKAQCQLRLSRTHIKACLVLKTIVLAYLWTSFLDTAFSGVRTA
                     EEEQNFQGDRKAIRALAGDYHVQFSFRETVSFSKEYRLAAPYEPDGYEIIRVIRDDGA
                     LISLQHILCEYLCNLAGLRHSGC"
     gene            complement(1417..1632)
                     /locus_tag="VN647_04550"
     CDS             complement(1417..1632)
                     /locus_tag="VN647_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96335.1"
                     /translation="MPVVFESRALARGRLGPAVPPTENLNPAEMFAAHIKFSRARGLQ
                     AKLMRVTAAVPGLMKNGLQRDYRNNLY"
     gene            1658..1846
                     /locus_tag="VN647_04555"
     CDS             1658..1846
                     /locus_tag="VN647_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96336.1"
                     /translation="MRLDENIAEAVGTAITWTIGKPDMVVLRMNQRTLRITPRTDVST
                     SLAIDCRKRHEAARSQKV"
     gene            complement(1925..2131)
                     /locus_tag="VN647_04560"
     CDS             complement(1925..2131)
                     /locus_tag="VN647_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96337.1"
                     /translation="MSVSQTTIQIWRAPIVLSIVSAVGLISALLGDGIWDALSWVALT
                     APIAAVLWSVVRAISPRPLSDVLL"
     gene            complement(2131..>2890)
                     /locus_tag="VN647_04565"
     CDS             complement(2131..>2890)
                     /locus_tag="VN647_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWG96338.1"
                     /translation="EMFAGLWGTLFLGVMGLIFVASVVSGIVVYGPFMRKLPFGTLRG
                     TRSPRLAWLDLHNLLGIVTVSWALVVGVTGVINTLDRPLLSYWQMTEITDMLTAWKDK
                     PAPTKVVPVDEVIRTVEAVAPDLAVRFIAFPGTPFAGPHHYMVFVRGQTPLTGRLLTP
                     MLIHAETAQVSDSRHLPWYLTVLLLSQPLHFGDYGGLPLQLLWAVLDLITIVVLISGL
                     YLWWRKRKSSVEDLLMQTKWTAESDFPLEPSARS"
BASE COUNT          683 a          857 c          741 g          609 t
ORIGIN      
        1 cccggcaaga tgcctctcac ccccttagtg acagacctca ctcgaactgg atcgtggtgt
       61 caacgaagct tgccccaacg atggttgtgc cacgatcgcc gcaagccgcc gtattgtttc
      121 ggcaaacagc tgaaactcgt cacgcgtgaa gtggagcgtg atggaaccta acgtcacatg
      181 gagcttgccg cacccgcaaa ggaccagctt catctcccca tcggttgata acgtactttt
      241 cacagtgctt tcccctttcg tcgatcaatg gtcggtatta tctgcaggcg ccacgattca
      301 tcaatctgac ggctcgactg gacgatactt caatctctct gtgacaccaa ccgcaattca
      361 cgcacctcct acgactcgct tgatagtaat cgagcacttg ctcctggacc acgaagcctc
      421 cgcattttcc gcagattgat ttcatgcttc cctcctttga caattctcac atgatattct
      481 gtgtcctcgc cctccgatca gtgtcctcta ttccaatctt ctctttgtat caatgtcctg
      541 atcatcaaac ttgaccacgt ggcttgtcgt cgatgtcccg atgatgtcgc gggcacgcgc
      601 gattgcggtt tgcacatcga ggcgtgtatg tctcacgtcg tcggccaacg ttagaagcct
      661 cgtatcaagt cgtcccgatt cagttccacg ttgcacattc aatgaaccgg gtcgcctgag
      721 caagtccctc catacggcgc ggacttcatc ccaataggcg tgcgtgtcct tccaataccc
      781 ctcagcaacc tgaatgtcga aggcctgcga ggttgcatag gtattcacag agaatgtgct
      841 gtaaactaat gagggcgcca tcatcacgaa tcacacgaat gatttcgtag ccgtccggtt
      901 catagggggc ggcaagccta tattccttgg agaatgaaac cgtttccctg aaactaaact
      961 gcacatggta gtcaccggca agtgcacgga tggctttccg atcaccttga aaattctgtt
     1021 cttcttccgc cgtccgaact cccgagaaag ccgtatcaag gaacgatgtc cacaagtacg
     1081 caagcacgat cgttttcagc acaagacagg ctttgatatg tgtgcggctc agcctgagtt
     1141 gacattgcgc cttcgtcatg ggcctcctcc aaattcatcg gcctcaacaa aaactcccac
     1201 ggcgtcttcc gacaccttgt ccccgtgacc gttagtgaac ggcgggatct tgcgcctccg
     1261 cgcaagcatt tatttgatat aagttctcaa tatcatttta aaagaagtgt tgtcaaccct
     1321 cgcacctggt aagagtttgt gccgagtata aatgtaaaac cggcccactg agaaatgtgg
     1381 acgatcattc ttatgcgaga gagttattcc accgactcaa tacaaattgt ttcggtaatc
     1441 gcgctgcagg ccatttttca tcaggcccgg tactgccgcc gtcacacgca tcaatttcgc
     1501 ctgcagacca cgtgccctgc tgaatttaat atgcgcggcg aacatttcag cgggattaag
     1561 attctctgtg ggcggcacgg caggccccag ccgaccccga gccagagcac ggctctcgaa
     1621 gacaaccggc atcgggaatc agcatgaccg ctgcgatatg cggctggacg agaatattgc
     1681 ggaagctgtc ggcacggcaa ttacctggac gatcggcaaa ccagacatgg tcgtcttgcg
     1741 gatgaatcag cgcaccctgc ggatcacccc tcggactgac gtcagcacgt ccctggctat
     1801 cgattgtcgc aagcgccatg aagcggctcg cagtcagaaa gtctgagaca tcggctggaa
     1861 tatcagacgg aggcggcgca gcccagaagt tcgagcgtag caacgccttc gcgcagtgcc
     1921 tgggtcacaa gagcacatca gagagagggc gaggagaaat ggcgcgtacg accgaccaca
     1981 gaacggcagc aatgggggcg gtgagtgcca cccaggacag agcatcccag atgccgtcgc
     2041 ccaagagggc cgatatcaac ccaactgcgc tgacaatgct caacacaatc ggtgccctcc
     2101 agatctgtat ggttgtctgt gaaacactca tcaggacctc gcgctcggct ccagcggaaa
     2161 atcgctctct gcggtccact tcgtctgcat caatagatcc tccacggaac tcttgcgctt
     2221 ccgccaccag agatataggc cactgatcag gactacgatg gtaatgaggt ccagcaccgc
     2281 ccacaacagc tgcaagggca ggcccccata gtcaccgaag tgcagcggtt gcgagagcag
     2341 caacacggtg agataccagg gcagatgtcg actgtcactg acttgcgccg tctcggcatg
     2401 gatcaacatc ggggttaaca atcgcccggt caacggcgtc tggccccgca caaagaccat
     2461 atagtggtgc ggaccggcaa agggcgtccc gggaaaggcg atgaagcgaa cggctaaatc
     2521 cggcgccaca gcttccactg tccggatgac ctcgtccacc gggactactt tcgttggagc
     2581 aggcttgtct ttccaagccg tcagcatgtc ggtgatttcc gtcatctgcc aatagctcag
     2641 gagcggccgg tccagggtgt tgatgacacc ggtcacgccg acgaccaagg cccagctcac
     2701 cgtgacaatg cctagaagat tgtggagatc gagccaggcg agacgggggc tgcgtgtccc
     2761 gcgcaaagtc ccgaacggca atttccgcat gaagggacca tagacgacaa tgcccgaaac
     2821 gaccgaggcc acaaagataa gccccatgac cccgagaaac agggtccccc acaggccggc
     2881 aaacatttcg
//
LOCUS       DATNDD010000190         3284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_666311, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000190 DATNDD010000000
VERSION     DATNDD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666311"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VN647_04570"
     CDS             complement(<1..625)
                     /locus_tag="VN647_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWG96339.1"
                     /translation="MLERSIIVSAHPDDEVLWFSSILDKVNEIVICFVNNESYPEWNV
                     GRRKSLAVYPMRNLSILNIDQSEVFNGGDWNNPLMTEYGMEIADKKLSDRSEQYKKNY
                     ERLRQQLEKHLIGFFNVFTHNPWGEYGHEEHIQVYRAVKSLQGNLKFNLWYSNYVSNK
                     SFKLMLGHLDRFHFDYASLETNKVLARDIRDIYKRNKCWTWYDHWEWY"
     gene            complement(757..2049)
                     /locus_tag="VN647_04575"
     CDS             complement(757..2049)
                     /locus_tag="VN647_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG96340.1"
                     /translation="MGDIAIRVEEIGKKYRIGKPEKYKTLRDTLASSFTAPFLRVGQL
                     LRGEVAVDAGQDEQFWALKEVSFEVRQGDVIGVIGGNGAGKSTLLKILSRITEPTTGF
                     AEIHGRVASLLEVGTGFHQELTGRENTYLNGAILGMNKKEIDRKFDEIVAFSEVEKFI
                     DTPVKHYSSGMYLRLAFAVAAHLEPEILIVDEVLAVGDAGFQQKCLGKMGAVAKEGRT
                     VLFVSHNMGAITQLCGRAVQLEKGRLKRTGSAGEVVTAYLSSVAGAELKSSWSNEPST
                     PDGSDVRFRSVRLLSPDEQPLSIINFNESLLVEIVYEVKVPIRNLSVTCLLYDSMNHL
                     VFESMDTDMPEWNGCIREPDSYCATTKIPPLLLRPGRYHLSFAAFVEGVRVIEQLRRV
                     LTFDVSGVGYALQPKRDGIVSPVLDWKVCRFSENGRMV"
     gene            complement(2122..2952)
                     /locus_tag="VN647_04580"
     CDS             complement(2122..2952)
                     /locus_tag="VN647_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG96341.1"
                     /translation="MQSNTQVIRIEPSKGRVALQLQELWAYRELLYFLIWRDVMVRYK
                     QTALGAAWAIIQPIFTMLVFSLFFGRLGKIPSDGIPYPIFTYAALVPWTFFSQGLSQA
                     SNSLVGSANLIKKVYFPRLCVPIAAVTSGLIDFALAFMVLLGMMLYYGILPGGKVIWL
                     PVLLLLTLTTSLGVSLWLSALNVHFRDVRHIIPFLTQLWLFATPIAYPSSLLSEPWRT
                     LYSINPMVGVVEGFRWALLGAETAPGPMVVVSFLAALAILVSGVFYFRRLEKTFADIV
                     "
     gene            complement(3007..>3284)
                     /locus_tag="VN647_04585"
     CDS             complement(3007..>3284)
                     /locus_tag="VN647_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96342.1"
                     /translation="STIDLGTGQLVSVEAMVRQIVEIMGVSIEPQFGALSSRLCEEVR
                     AADIEQTRRVLGWQPKISVYEGIKRTVEWHRSSGPISAVMPSAVKRD"
BASE COUNT          864 a          904 c          729 g          787 t
ORIGIN      
        1 tgtaccattc ccagtgatca taccatgtcc agcatttatt cctcttataa atatccctaa
       61 tatcgcgtgc caggactttg ttcgtttcga gggaagcata gtcgaaatga aatctatcca
      121 gatggcccag catcaacttg aacgatttat tgctaacata gttggagtac cataagttaa
      181 atttcagatt gccttgtaaa cttttgacgg ctcgatatac ctgtatatgc tcttcatgtc
      241 catactcccc ccaggggtta tgggtaaaca cgttgaagaa cccaatcaag tgcttttcga
      301 gctgctgcct cagcctctcg taattctttt tatattgctc actccgatca gaaagtttct
      361 tatcggcgat ctccattcca tattccgtca tcagcggatt gttccaatcc ccaccgttga
      421 acacctctga ctgatcgatg ttgagaatgg atagattcct catcgggtat actgccaagc
      481 tctttcgcct tccgacattc cactcagggt aagactcgtt gttcacaaaa cagatgacga
      541 tctcattgac tttatccaag atggagctaa accataacac ctcgtcatct gggtgggccg
      601 atacgatgat ggatctttca agcatggcag gaaacggcat catccatcta gattaggaaa
      661 cgcgttgttg gtcaccgcca gaactgagga gattcctttg ggcgcttctc aatattcggg
      721 tttcgattag gtcctattga gcggcagaag agaaggctag accatcctgc cgttctcact
      781 gaatcgacac accttccaat caagcacggg cgagacgata ccatcacgtt tgggctgcaa
      841 ggcatacccc actcctgaca catcaaacgt taatactctc cgaagctgct caataaccct
      901 cactccctcg acaaatgcgg cgaacgataa atgatagcga ccaggtctaa ggagaagggg
      961 ggggattttt gtggtagcac aatagctatc gggctctcta atgcatccat tccattccgg
     1021 catatcggta tccatcgact cgaacacgag atgattcatc gaatcataaa gaagacatgt
     1081 gacggaaaga tttcttatgg gaacctttac ttcatagaca atctcgacca gaagcgactc
     1141 gttgaagttt ataatggaaa gaggctgttc atcaggcgat aacaaccgta cggatctaaa
     1201 tcgaacatca gagccgtcgg gcgtggaagg ttcatttgac cagctcgact tgagttctgc
     1261 cccggctact gacgacaaat acgcggtcac tacctcacca gcagaaccgg tccgtttcaa
     1321 cctccccttc tcaagttgca cggctcttcc gcaaagctgc gtaatggctc ccatgttgtg
     1381 actgacaaac aagacggttc gaccctcttt ggccactgct cccatttttc cgagacattt
     1441 ttgttggaat cccgcatctc cgacagccag cacttcatca actatcagga tctccggctc
     1501 gagatgcgcc gcaacggcaa aagccaatcg aagatacatt ccgctggaat agtgctttac
     1561 aggagtatcg ataaacttct cgacttcgga aaaggcgaca atttcatcga attttctatc
     1621 gatctccttc ttattcatgc cgaggatcgc accgttcaaa taagtattct cccttcccgt
     1681 taattcctgg tgaaacccgg tcccaacctc caagagtgag gcgacacgtc cgtgaatttc
     1741 agcaaagccc gtcgtcggct cggtgatccg tgagagtatc tttaagaggg tgctcttgcc
     1801 ggcaccgttg ccaccgatca ccccgatgac atcaccctgc ctcacctcga aagaaacctc
     1861 cttcaacgcc cagaactgct catcctgtcc ggcgtcgacc gccacctctc ctcgtaacaa
     1921 ttgtcccacc ctaagaaatg gagcagtgaa cgacgacgcc aacgtatccc gcagcgtttt
     1981 gtatttttcg ggtttaccaa tacggtactt cttgccgatt tcctcaactc gtatggcgat
     2041 atcacccatg attcctctct gcaggccagg agtagacacg tcgtctgcgt cttactgctg
     2101 cgaacgcccg ctgccacgac atcaaactat atcagcaaaa gtcttttcca ggcgacggaa
     2161 gtaaaatacg ccgcttacca aaatcgccag ggcagccaaa aaagaaacaa ccaccatcgg
     2221 tcctggagcg gtctctgccc caagcaaagc ccaacggaac ccctcgacta ctccgaccat
     2281 tggattgatg ctatagagag ttcgccatgg ttctgacaat aggctgctcg gatacgcgat
     2341 cggggttgca aagagccaga gttgtgtcaa aaatgggatg atatggcgca cgtcgcggaa
     2401 atggacattc aatgcagaaa gccagagcga gacaccaagt gacgtagtaa gggtcagcag
     2461 aagcaacaca gggagccaga tcacttttcc cccaggtaga ataccataat agagcatcat
     2521 tccgagcaac accataaagg caagcgcaaa atcgatgagc ccagaggtca ctgccgcgat
     2581 cgggacgcac agtcgtggga aatagacctt tttaatcaga ttcgcactgc cgaccaggct
     2641 gttggacgct tgactcaacc cttgggaaaa gaaggtccat ggaaccaagg ctgcatacgt
     2701 gaagattgga tacggaattc catcggaagg gatctttccc aatcgaccga aaaagaggct
     2761 aaagacgagc atcgtaaaga tgggttggat aatcgcccat gctgcaccca aggccgtctg
     2821 tttatagcgc accatcacat cacgccagat caagaagtag agcagctccc gatatgccca
     2881 taactcttgc aattgaagcg ccacccgtcc cttggacggc tcaatccgaa tgacttgagt
     2941 atttgattgc atcagatggc tcgtgcagtt tttttgatgg tgacagtgga gatgaaacgt
     3001 gccatgtcag tcccgcttaa cggcagacgg catcacagcg cttatcggac ctgacgatcg
     3061 gtgccattcg actgttcttt taatgccttc atagactgat atcttcggtt gccaacctaa
     3121 cactcgacgt gtttgctcta tgtcggcggc tcgcacctcc tcgcaaagcc gactcgatag
     3181 cgccccaaac tgcggctcga tcgatacccc cataatttcg acaatctgcc ggaccatagc
     3241 ttctaccgaa accaactgcc ctgtccccag atcaatcgtc gagc
//
LOCUS       DATNDD010000191         5831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3666068, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000191 DATNDD010000000
VERSION     DATNDD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3666068"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /gene="uvrB"
                     /locus_tag="VN647_04590"
     CDS             <1..1680
                     /gene="uvrB"
                     /locus_tag="VN647_04590"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWG96343.1"
                     /translation="YIAKDASINDAIDQMRHSATTELLQRNDVLIVSSVSCIYGLGSP
                     EVYHDMLIYLEEGVETRREKILSKLVEIQYERNDVDFHRGTFRARGDVIEIFPASSEA
                     KSVRIELFGDVVDAIHEIDPLTGKSLGKLPKIAIYPNTHYLIAPDRYERAITGIEEEL
                     EERLALFRKTGRLLEAQRLEQRTKFDLEMIRAMGYCHGIENYSRHLSGRRSGEPPPTL
                     LDYFPKDFLLIVDESHATVPQVGGMYEGDYSRKRTLVEYGFRLPSAVDNRPLKFSEFE
                     GCLNQAVYVSATPGSYELEHAGCDVVEQIVRPTGLMDPQIDVVPAKGQVDHLLGQVRS
                     EVAKGGRVLVTTLTKRMAEDLTEYYHDLAIKVRYLHSDIKTLERAEIVRDLRCGTFDV
                     LVGINLLREGLDLPEVSLVAILDADKEGYLRSYRALIQTAGRAARNLEGRVIFYGDVV
                     TDSMKQAIEETNRRRGIQAEYNRVHGITPVGITKGIPSLEYAVANMDYVRLDLAAESI
                     ETYRSSESTDQLIERLESEMKAAAKELAFERAAELRNQIRSLRLQALNAKS"
     gene            complement(1677..1937)
                     /locus_tag="VN647_04595"
     CDS             complement(1677..1937)
                     /locus_tag="VN647_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HWG96344.1"
                     /translation="MRKSPWVLLIFVLIGGLLGGILGEILHAMAPQGTIQSIFATNYN
                     PGIDPPLAINLVLIKLVFGFNIKVNILSILGMFIGIYLYKHV"
     gene            2166..3512
                     /gene="ffh"
                     /locus_tag="VN647_04600"
     CDS             2166..3512
                     /gene="ffh"
                     /locus_tag="VN647_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWG96345.1"
                     /translation="MLDALSDKFEKILKKLRGQGVLTEQNITEALKDVRFALLEADVN
                     FKIVKEFIDRVRQKAVGQEVLESLTPGHQVVKVVWDELKAMMGHERAGLALSSKPPTI
                     LMMVGLQGAGKTTASGKLARLFKNQGKRVLLVAADPRRPAAGEQLSALGRDLGVEVHR
                     ADHVQASQADVVRICRAGVERGRDQGFDLIILDTGGRLHIDDELMNELVAVKTDVSPH
                     EVLLVADAMTGQDAVTMAGQFDQKVGLTGIILTKVEGDARGGAVLSIRAATGKPIKFL
                     GVGEKLDALEPFHPDRMASRILGMGDVLSLIEKAQESITREQAEEAQKRLTGNTFTLE
                     DFRTQLGQMNRMGSFEQILGMLPGGQKLKQAIEGDKPEREIGRVIAVIDSMTPRERRD
                     HTIINGSRKKRIARGSGTTVQDVNRLIKQFLSAKKLAKAMTGAGGRRQLAQLMQSR"
     gene            3558..3851
                     /gene="rpsP"
                     /locus_tag="VN647_04605"
     CDS             3558..3851
                     /gene="rpsP"
                     /locus_tag="VN647_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWG96346.1"
                     /translation="MAVHLRLARTGRHKRPMYRVVAADSRKARDGRFLEILGIFDPLK
                     EAGVPELKQERVLTWLRHGAQPTVTVRTLLRRAGVWKQFEAEKAAQKKASGKS"
     gene            3805..4377
                     /gene="rimM"
                     /locus_tag="VN647_04610"
     CDS             3805..4377
                     /gene="rimM"
                     /locus_tag="VN647_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWG96347.1"
                     /translation="MRLKKPRRKKRLENPDITMVYQQETVTVGRIERPFGIKGEVKVR
                     PLSDVPGRFEGLRSVSLLARNGQTLETNVTHVRRVGSEFILGLTGLTTPEDASLWRGG
                     FIQTIRGSVPKLPEGQYYECDLVGLAVFTGEGEPIGVLEEIWDLPGNPLFVVRQGAKE
                     ILIPAAKELVSTVDLTARKMIVRLIDGLGE"
     gene            4379..5164
                     /gene="trmD"
                     /locus_tag="VN647_04615"
     CDS             4379..5164
                     /gene="trmD"
                     /locus_tag="VN647_04615"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWG96348.1"
                     /translation="MLRFEVLTLFPGMFAPVLAHSMLKRGQEKGLLTVKVHNLRDFTA
                     DRHKVVDDMPYGGGAGMVMKAEPILLAVAALRNEAQANGGDIRMMFPTPHGRPFTQDY
                     AQQLSGERRRIVILCGHYEGVDERVRLTLAPEEVSLGDYVLTGGELPALVLIDAAARL
                     VPGVLGDPSSVLEESFADTLLEYPQYTRPAEIRGIGVPDVLLSGHHEAIRLWRRKQAL
                     RRTYVRRPDLLKDRMFTKEDQQLLDELMSEGLLTAPTSRREEG"
     gene            5169..5573
                     /gene="rplS"
                     /locus_tag="VN647_04620"
     CDS             5169..5573
                     /gene="rplS"
                     /locus_tag="VN647_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWG96349.1"
                     /translation="MNQLERIQRSLTKKSVPSFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGSLNTETFTVRKLSYGVGVERIFPVHSPIVSKVDVVRQGRVRRAKLYYLRGK
                     KGRFAKVEEREFVGESKPSAQPTASEKEAVTA"
     gene            5637..>5831
                     /locus_tag="VN647_04625"
     CDS             5637..>5831
                     /locus_tag="VN647_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="cleaves the the RNA-DNA junction of a RNA-DNA/DNA
                     heteroduplex; does not have RNase H activity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWG96350.1"
                     /translation="MGPTEEFERAARLCGYRRIAGIDEAGRGPLAGPVVAAAVVLPTR
                     CRLSGVDDSKQLSEGQRDRLY"
BASE COUNT         1318 a         1373 c         1831 g         1309 t
ORIGIN      
        1 tatatcgcga aggatgcctc gatcaacgat gccattgatc agatgcgcca ctccgccacg
       61 accgagctct tgcagcgaaa cgacgtgctg atcgtatcct ccgtgtcctg tatctatggg
      121 ctgggttcac cggaggtcta ccacgacatg ctcatttatt tggaggaagg cgttgaaacg
      181 aggcgagaaa agatcttatc gaaactagtg gagattcaat acgagcgcaa tgatgtggat
      241 tttcatcgcg ggacgttccg ggcacgtggg gacgtgatcg agattttccc tgcctcgtcc
      301 gaggccaagt cggtgcgcat cgagctgttc ggagacgtcg tggatgccat tcatgagatc
      361 gatccactca ccgggaaatc attgggcaag cttcccaaga ttgcgatcta tccgaacacc
      421 cattacctca tagctcccga tcgttatgag cgggccatca ccggtatcga ggaagaattg
      481 gaggaacgct tggctttgtt caggaagacg ggtcggctat tggaggctca acggcttgag
      541 cagcgcacta agttcgattt ggagatgatt cgagccatgg ggtattgcca cgggatcgaa
      601 aattactcgc gtcacctcag cgggcggagg tcgggtgagc cgcctcccac attgttggat
      661 tatttcccga aggattttct gttgatcgtc gatgagtctc acgcgactgt tccacaagtc
      721 ggcgggatgt atgaggggga ttactccaga aaacggacat tggtggagta tggatttcga
      781 ctcccgtcgg cagtcgacaa ccgtccattg aagttttccg agttcgaggg ttgtctcaat
      841 caggcggtgt atgtgtctgc aacgcccggt agctacgagc tggagcatgc cggatgcgat
      901 gtggtcgaac agatcgttcg cccgaccggt ctgatggacc cgcaaatcga tgtcgtgccg
      961 gccaagggac aggtggacca cctgcttggc caggtccgct cggaggtggc caagggcggt
     1021 cgggttctcg tgacgacatt gacgaagagg atggcggagg atctgaccga gtattatcac
     1081 gacctggcaa tcaaagtgcg ttatctgcat tccgatatta aaactcttga gcgagccgag
     1141 atcgtccgcg acctccggtg cgggacgttt gacgtgctag tggggatcaa tttactgcgc
     1201 gaagggctcg atcttcccga agtaagcctg gtggccattc ttgatgccga taaggaaggc
     1261 tatctgcgtt cgtaccgcgc gttaattcaa acggccggac gagcggcgcg caatctcgaa
     1321 ggccgggtca ttttttacgg tgatgttgtg acggattcga tgaaacaggc gatcgaagaa
     1381 acgaaccgtc gccgtgggat ccaagcggag tacaaccgag tccacgggat tactccggtc
     1441 ggtattacga aaggtattcc ttctcttgag tatgcggtgg ccaatatgga ctatgttcgg
     1501 ttggatcttg cagctgaatc gatcgagaca tacaggagtt cggaatcaac ggatcaactc
     1561 atcgaacgac tggagtcgga aatgaaagct gccgccaagg agctggcctt tgagcgggct
     1621 gcagagctac gcaatcaaat tcgatcgctc cggcttcaag cgttgaacgc caagtcctag
     1681 acgtgtttgt agaggtagat cccgatgaac atcccgagga tgctgagaat gttcacttta
     1741 atgttgaagc caaagacgag tttgatcagg acaaggttga tggcgagcgg tgggtcgata
     1801 ccagggttat aattcgtcgc gaaaatactt tgaatggtgc cttgaggtgc catagcgtgg
     1861 agaatttccc caagtatccc gccaaggagc cctccgatca gcacaaagat gagcaaaacc
     1921 cagggcgatt ttctcacgca accattcttc tttctttatg agtttccgta cagtggttct
     1981 ggatggctga agggcaccat atccgaaggt tttcggcatg tcaataaaac tactgacttg
     2041 ggatgccttg acttgccctg cttggtccga tacactcttc gatgctcttt tcgcaggatt
     2101 ttcgaggcga tcggaggtct ggtgcaaggc ttcgatggcc tctttctttg tgatgatgtg
     2161 atgtcatgct tgatgcgttg agcgataagt ttgagaaaat tttgaagaaa ctccgagggc
     2221 agggtgtgct cacggagcag aatatcacgg aagcactgaa agatgttcgg tttgcgcttc
     2281 ttgaagcaga tgtcaacttt aagatcgtca aagagttcat tgatcgggtc cgtcaaaagg
     2341 ccgtgggcca ggaggttctg gagagtctga ctcccggaca tcaagtcgtc aaagtcgtct
     2401 gggatgagct caaggcgatg atggggcatg agcgggcggg acttgccctg agctcaaagc
     2461 cgccgaccat cctgatgatg gttggattgc aaggagccgg aaagaccacg gccagcggca
     2521 agcttgcgcg tctgtttaag aatcaaggca agcgagtgct gttggttgct gccgacccgc
     2581 gtcgtcctgc ggccggtgag caactgagtg cccttggtcg ggatctcgga gtagaggttc
     2641 accgtgcgga tcacgttcag gcttctcaag cggatgtggt aaggatctgc cgtgcagggg
     2701 tagagcgagg acgagatcag ggttttgatc tcatcatcct ggataccgga ggccgattgc
     2761 atatcgatga cgagttaatg aacgaacttg tcgccgtgaa aacagacgtg tctccccatg
     2821 aagtgctgtt ggttgccgac gcgatgaccg gacaggatgc ggtcaccatg gcgggacagt
     2881 tcgatcaaaa agtcgggttg accggtatta ttctgaccaa ggtcgaaggg gatgcacgcg
     2941 gaggtgcggt gttgtcgatt cgggccgcaa ccggcaagcc catcaagttt cttggtgttg
     3001 gggaaaagct ggatgcattg gagccgtttc atccggaccg aatggcttcg cgtattcttg
     3061 ggatgggcga cgtactgtcg ctcattgaga aagcccaaga gagtatcact cgggagcagg
     3121 ccgaggaagc tcagaagcga ctcaccggta ataccttcac cctggaggat ttccggaccc
     3181 agctcggtca gatgaaccgc atgggttcgt ttgagcagat tcttggcatg ctcccaggtg
     3241 gacaaaaact taagcaggcg atcgaagggg acaagccgga gcgagagatc ggccgtgtga
     3301 tcgccgtgat cgattcaatg accccgcggg aacgtcgcga tcatacgatc atcaatggaa
     3361 gccggaagaa gcggatcgcc cgtggcagcg gaacgacagt gcaggatgtg aatcgcctca
     3421 tcaagcagtt cttgtccgcc aagaagttgg caaaggcgat gaccggtgcg ggagggcgcc
     3481 gacagctcgc ccaactcatg caatctcgat aagcgatcat gaccgatagt gcgaattcag
     3541 cgtaaaggag gacagttgtg gctgtacatt tgagactggc tcgaacggga agacacaaac
     3601 gaccgatgta tcgggtggtg gccgccgatt cacggaaagc gcgcgacgga cggttcctgg
     3661 agattcttgg aatttttgat ccattgaaag aagccggcgt gccggaactg aaacaggagc
     3721 gtgtcctgac gtggctgcgg catggcgctc aacccacggt gaccgtacgg acattgttac
     3781 gcagggccgg agtctggaag cagtttgagg ctgaaaaagc cgcgcagaaa aaagcgtctg
     3841 gaaaatcctg acatcacgat ggtgtatcaa caggaaaccg tgacagtggg acggatcgaa
     3901 cggcccttcg gcatcaaggg ggaagttaag gttcgtccgc tgtccgatgt tccgggccga
     3961 tttgaagggt tgaggagtgt gagtctcctc gcaaggaacg gacagactct tgagaccaat
     4021 gtcacccatg taaggcgcgt ggggtctgaa tttattcttg gattgaccgg tttgaccacc
     4081 ccggaggacg cgagtctctg gcgaggcggg ttcattcaga caattcgcgg gtccgtgccc
     4141 aagttgccgg aggggcagta ctatgagtgt gatctggtcg gtcttgccgt cttcaccggg
     4201 gagggggagc cgattggtgt gctggaggag atttgggact tgccgggaaa tcctctattc
     4261 gttgttcgcc agggggccaa agagatcttg atacccgcag cgaaagaact ggttagtact
     4321 gtcgacctga cagctcgaaa aatgatcgtc cggttgatcg acggattggg ggagtagcat
     4381 gttgcggttc gaggttctga cattgttccc cgggatgttc gcgccggtct tggcgcacag
     4441 catgctcaaa cgaggccaag aaaaggggct tctcaccgtg aaggtgcaca atttgcggga
     4501 cttcacggcg gatcgccaca aagtagtcga tgatatgccg tatggaggtg gagccggcat
     4561 ggtcatgaag gctgagccga tccttcttgc ggttgctgcg ctccggaatg aagcgcaggc
     4621 aaacggtgga gacattcgga tgatgtttcc aactcctcac gggcggcctt ttacacaaga
     4681 ctatgcacag cagttgtcag gtgagcgtcg ccgaatcgtc attctgtgcg ggcactatga
     4741 gggagtggat gagcgtgtcc gtctgacatt agctccggag gaagtctcac tcggagatta
     4801 tgtgttgacc ggcggtgaat tgccggcgct tgtattgatc gatgcagcgg ccagacttgt
     4861 tcccggcgtc ttgggtgacc ccagttctgt cttggaagag tcgtttgccg acacgttgct
     4921 ggagtacccg caatacacga ggccggcaga aatccgagga atcggcgtgc ccgatgtctt
     4981 gctctcgggg catcatgagg ccattcggtt gtggcgtcga aaacaagcgt tgcgcagaac
     5041 gtatgtgcga cgccccgatc ttctgaaaga tcggatgttt acgaaggagg atcaacagtt
     5101 gttggatgaa ttgatgagcg aaggtctttt gacggccccg acatcacgcc gggaggaggg
     5161 ttaagtccat gaatcagttg gagcgaattc agcggtcgtt gacgaagaag tcagtgccga
     5221 gctttgagat cggggatacc gtcagggttc acgtcaaagt cgtcgaaggc gagaaagaac
     5281 gcattcaggt ctatgaagga accgtgatcg cgcgcaaggg gagtttgaat actgaaacat
     5341 ttacggttcg aaaactttcg tacggggtcg gggtcgaacg gatttttccg gtgcattctc
     5401 cgatcgtctc caaagttgat gtggttcggc aggggcgcgt tcggcgcgcg aagctatact
     5461 acctgcgtgg caaaaagggg aggttcgcga aggttgaaga gcgcgagttt gtcggagaga
     5521 gcaaaccgtc ggcacaacca acggcttctg agaaggaggc tgtgacggct taggccgctt
     5581 ccgcgtatgt gttccctcac ggcaatgcga tggcgcgatc ggtgaaggga tgttcaatgg
     5641 gacccaccga agagttcgag cgggcggccc gcctgtgtgg gtatcgtcgt attgccggta
     5701 ttgatgaggc cggacgcggt ccgttagccg gtccggtcgt cgccgccgct gtcgtcttac
     5761 cgacccgatg tcgactctca ggggtcgatg attccaaaca actttctgaa gggcagcgag
     5821 accgattata t
//
LOCUS       DATNDD010000192         5540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3167956, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000192 DATNDD010000000
VERSION     DATNDD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3167956"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..301
                     /locus_tag="VN647_04630"
     CDS             41..301
                     /locus_tag="VN647_04630"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HWG96351.1"
                     /translation="MSSDTIHVYDTWVNGKSGRIHFDVMTTDEATALKLAKEYLVSIG
                     EPNAPVTTKECQFCHSEPLFMFSAEQQKQTKEKGGFIVPMPA"
     gene            complement(376..927)
                     /gene="lspA"
                     /locus_tag="VN647_04635"
     CDS             complement(376..927)
                     /gene="lspA"
                     /locus_tag="VN647_04635"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWG96352.1"
                     /translation="MSASGLRNLALASVTGSIIFLDQLTKQQIMQTMRLHESISVIPN
                     LLSLTYIRNPGAAFGLLAGSSNAFRMVFFGLTSLFALGLLGTILLRMPEEDWMGRVSV
                     AGILGGAIGNLIDRLRFGEVIDFLDVYVENYHWPAFNVADSAITVGVIFLIIHFAFEK
                     RADPPTASETSSVPQDRQHDPMR"
     gene            complement(924..3737)
                     /gene="ileS"
                     /locus_tag="VN647_04640"
     CDS             complement(924..3737)
                     /gene="ileS"
                     /locus_tag="VN647_04640"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWG96353.1"
                     /translation="MDYKSTLNLPKTDFPMKANLPQREPEMLAWWEQQKLYEQIQAMG
                     HGRPRYVLHDGPPYANGRIHIGHGLNYVLKDIIVKSKTMSGYQTPFVPGWDCHGLPIE
                     HQVMKDLGEKKRDLDALAIRKLCKEYAEKYVTLQGEEFKRLGVLGDWQQPYLTMTPSY
                     EATIIREFGKFVERGGVYKGLKPVLWCTQDQTALAEAEVEYDDHSSPSIYVKFPIVTS
                     PSVLGKTFPGISFPEGAKPVSIVIWTTTPWTLPANQAVCLHRDIDYAFVQVGDELLVM
                     AEKLIESVVKACKFEDYRVLGVKKGGEGFEGLETQRPLSTGLSPILLGDFVTLEQGTG
                     CVHIAPGHGMEDYLLVLEHNAKASSGERLEILAPVDNAGRFTSVVKEFAGQHVLKANP
                     KIVDYLQANGRLLGHGSLNHSYPHCWRCKSPVIFRATEQWFVSMETNDLRKEALAEIE
                     RVRWLPGYGRDRINGMIENRPDWCLSRQRVWGVPIPGFTCKTCRHVLADPVVIEHIAV
                     LMEAKGADLWFERSAGELLPTGTKCPQCGGDQFEKEQDILDVWFESGVSYAAVLKPRK
                     WWPADLYLEGSDQHRGWFHSALLAGVVTDQRAPYKTVVTHGFVVDDHGKKMSKSAWNG
                     VAPHEIIKQHGAEIFRLWVAAQNYQDDIRISPTIMAQLIEAYRKIRNTCRFLLSNLYD
                     FDPVTHRVPYEKLPELDRWALLRLGELTTKVRQAYEDFEFHTIFHALNNFCSVDLSAV
                     YVDILKDRLYTFRKEAHERRSSQTVLYDIVVAMAKLMAPVLSFTAEEIWRTLAAQVPG
                     GMETASVHLASFPEIDPAWPDAKLAERWERLLKYRTQVQGVLEESRRGKTIGSSLEAH
                     VYIEANADNFRFLKPYEQDLSALFIVSKVMLTQSPEGGEPLRVTVSKSQAGKCERCWN
                     YREAVGKDAVHPTLCDRCVEAIR"
     gene            complement(3819..4922)
                     /gene="nadA"
                     /locus_tag="VN647_04645"
     CDS             complement(3819..4922)
                     /gene="nadA"
                     /locus_tag="VN647_04645"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWG96354.1"
                     /translation="MTVTAAVSRPITDYQTQSTEELFRRTAAAKSTLGERVMILGHNY
                     QRDEVIQHADFRGDSLLLAKLAAERSERPYIVFCGVHFMAETADILSRSQQTVILPDM
                     AAGCSMADMAAIEQVDQCWEALGRVVPVEDTVMPAVYVNSAAVLKAFCGEHGGITCTS
                     SNAKAVIEWCWARREKILFFPDEHLGRNTANKMGIPREQMIVWDPYQPHGGNTKEAIK
                     RAKLILWKGHCSVHQMFQPVHVDHFRKQYPDGNVIVHPECHEDVVNKADRIGSTEFII
                     RTVTAAPAGTTWAIGTELNLVNRLKHELTDKKVFFLSSTVCQCATMFRIDAAHLCWAL
                     ENLAEGHVVNHIVVPEDDKQWAKVALDRMMTIS"
     regulatory      5091..5193
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5305..>5540
                     /locus_tag="VN647_04650"
     CDS             5305..>5540
                     /locus_tag="VN647_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWG96355.1"
                     /translation="MSIQTNTHGSVNGNGIPTSPLTTTPFPASGKVYVNGTQSGVRVP
                     MREISLTQTTGANGTRSNNAPVIVYDTSGPYTDPA"
BASE COUNT         1304 a         1585 c         1423 g         1228 t
ORIGIN      
        1 gatcttggtc atattggtcg tccgatgaaa ggagtcggtc atgtcttcgg atacaattca
       61 tgtctatgat acgtgggtca acgggaagag cggtcgcatt catttcgatg tgatgacgac
      121 ggatgaagcc accgccctca aactcgccaa ggaatacctc gtcagcatcg gggaaccgaa
      181 tgcgccggtg acaacgaaag aatgtcaatt ctgtcacagc gaaccgctgt tcatgttctc
      241 ggcggagcaa cagaaacaaa ccaaagaaaa aggcggattt atcgttccca tgcccgctta
      301 accgtgaggg ataatagata ataaatgggt gcgtgttgag cgcaagttca acacgaccca
      361 ttcaagagta caggctcatc gcatgggatc atgctgccgg tcttgcggga cggaagaagt
      421 ttccgaagca gtaggaggat cggctcgttt ttcaaaggca aagtgaatga tcagaaagat
      481 cactccgacg gtgattgcag agtctgcgac attgaacgcg ggccagtgat agttctcgac
      541 atagacatcc aaaaagtcga tcacttctcc aaaacgcagc cgatcgatga ggttgccgat
      601 tgcgccgccg agaattcccg caacactgac acgacccatc caatcttctt ccggcatccg
      661 taacagaatc gtcccaagca gcccaagggc aaaaagcgac gtcaacccaa aaaagaccat
      721 ccgaaacgcg ttgctgcttc cggccagcag accgaatgcc gcccccggat ttcggatata
      781 ggtcaaactg aggagattgg gaatgacaga gatcgattca tggagccgca tggtttgcat
      841 gatctgctgt ttggtcagct gatccagaaa aatgatactg ccggtcaccg acgcgagcgc
      901 cagattgcga aggcctgacg cactcaacgg atcgcctcca cacatcgatc gcacagtgtt
      961 gggtggacgg cgtccttgcc gaccgcttct cgatagttcc agcaacgttc gcacttcccc
     1021 gcttgtgatt tcgacaccgt cacacgaagc ggttctccac cttccggact ttgcgttaac
     1081 atcactttcg agacaatgaa cagggcgctg aggtcttgtt cgtacggctt caaaaatctg
     1141 aaattatcgg cattagcttc gatgtacaca tgagcttcca aagaagaacc gatcgtcttc
     1201 ccccggcggc tctcttccag aaccccttgc acctgcgtac gatatttcag cagccgctcc
     1261 caccgctcgg ccagcttcgc atcgggccaa gctggatcga tctcaggaaa cgaggcgaga
     1321 tgcacactcg ccgtttccat ccccccgggt acctgcgcgg ctaatgtccg ccagatctcc
     1381 tccgccgtga aactcaacac cggggccatg agtttcgcca tagccacaac aatgtcgtac
     1441 aggacggtct gggagctgcg acgctcgtgt gcctccttgc ggaacgtata gagtcggtct
     1501 ttcagaatgt cgacatagac ggcgctcaaa tcgacggagc agaagttgtt caatgcgtga
     1561 aagatcgtgt gaaactcaaa atcttcatag gcttgccgca ctttcgtggt cagctcccca
     1621 aggcgcaaca atgcccatcg gtccaactcc ggcagttttt cataggggac acgatgcgtc
     1681 accggatcga aatcgtacag attactcaag agaaaccggc aggtgttacg gatcttccga
     1741 taggcttcga tgagttgcgc catgatggtg ggagagatgc gtatatcgtc ttgataattc
     1801 tgggcggcga cccatagccg aaagatttcc gccccgtgct gcttgatgat ttcatggggt
     1861 gccacgccgt tccaggccga tttcgacatc ttcttgccat ggtcgtcaac gacgaaacca
     1921 tgtgtaacca ctgtcttgta gggtgcccgt tggtctgtga ctacccctgc cagcaaggcg
     1981 ctatgaaacc agccgcgatg ctgatcggac ccttcaagat acaagtcagc cggccaccat
     2041 ttcctcggct tcaacacggc ggcatagctc acgcccgatt cgaaccagac atcgagaata
     2101 tcctgttctt tctcgaattg atctccgcca cactgcggac acttcgtgcc ggtcggcaac
     2161 agctcaccgg cagatcgttc aaaccagagg tctgccccct tagcttccat taagaccgcg
     2221 atatgttcga tcacgactgg gtcggcgagc acatgacgac atgtcttaca ggtgaacccc
     2281 gggatcggca caccccacac acgctgacgc gacaggcacc aatccggcct attttcgatc
     2341 atgccgttga tccgatcacg gccatagccg ggaagccaac ggactcgttc aatctccgcc
     2401 agggcttcct tccgcaaatc gtttgtctcc atggacacga accactgctc ggtcgcacga
     2461 aagatcaccg ggctcttgca ccgccagcag tgaggatagg aatggttcaa cgagccatgc
     2521 ccgagcaaac gtccattcgc ctgcaggtag tccacgattt tcggattggc cttcaagaca
     2581 tgttgacccg cgaattcttt cacgaccgac gtgaaccgtc cagcattatc gaccggagcc
     2641 aggatttcaa gtcgttcacc ggatgaagcc ttggcgttgt gctcaagcac aaggagatag
     2701 tcttccatgc catgtccggg agcgatgtgg acgcagccgg ttccctgttc gagggtcaca
     2761 aagtcaccga gcagaatcgg cgacaatccg gtggacaacg gacgttgcgt ctccaacccc
     2821 tcgaacccct ccccgccctt cttcaccccc aacacccgat agtcttcaaa cttgcaagct
     2881 ttgaccacgc tctcgatcaa cttctcggcc ataacgagaa gctcatctcc cacctgcaca
     2941 aatgcatagt cgatgtcgcg atgaagacag acagcttgat tcgccggaag ggtccagggt
     3001 gtggtcgtcc agatgacgat ggagaccggc ttcgctcctt ccggaaaaga aatgccaggg
     3061 aaagtctttc ccagaaccga cggtgaggtg acgatcggga atttcacata gatcgaggga
     3121 gatgaatgat catcatattc gacttccgct tctgccagcg cggtttgatc ttgagtacac
     3181 cagaggaccg gcttgagccc tttgtagacc cccccccgct caacgaattt tccaaactca
     3241 cgaatgatcg tggcttcata gctcggcgtc attgtcaagt agggctgctg ccagtcaccc
     3301 agcacaccca ggcgcttaaa ttcttctccc tggagagtga catatttttc cgcgtattct
     3361 ttgcagagtt tgcggatggc aagagcatcc agatcccttt tcttctctcc gaggtccttc
     3421 atcacttggt gctcgatcgg caggccgtga caatcccaac ccggcacaaa aggggtttga
     3481 taacccgaca ttgtcttcga cttgacgatg atgtccttta ggacgtagtt caacccatga
     3541 ccgatgtgaa tgcggccgtt ggcatacgga ggaccgtcat ggagcacata ccgaggccgt
     3601 ccatgcccca tcgcctgaat ctgctcatag agcttctgtt gctcccacca ggccagcatc
     3661 tccggctcgc gctgcggcag attggccttc atcgggaagt cggttttcgg aagattgagc
     3721 gttgatttgt aatccataga caatgagacc cggacattca ccggatgatc atacgggatc
     3781 gatggactat accggaagga aggggggagg caccagccct aactgatggt catcatgcgg
     3841 tcgagcgcaa ccttcgccca ctgcttgtcg tcttcgggca ccacgatgtg gttcacgaca
     3901 tggccttcgg ccagattctc cagagcccag cagaggtgcg ccgcatcgat tcggaacatg
     3961 gtggcgcact gacagaccgt ggatgacagg aagaacactt ttttgtcggt aagttcgtgc
     4021 ttcagtcgat tgacgagatt caattccgtc ccaatcgccc acgtcgtgcc ggccggcgcg
     4081 gcggtcaccg tacgaatgat gaactcggtc gatccgatgc gatccgcctt attgaccacg
     4141 tcttcgtgac attctggatg cacaatgacg ttgccgtccg gatactgctt gcggaagtga
     4201 tccacatgga ccggttgaaa catttgatgg acgctgcaat gacctttcca taagatcaat
     4261 ttggctcgtt tgatggcttc cttggtattc ccgccgtggg gttgataggg gtcccacacg
     4321 atcatctgtt cgcggggaat gcccattttg ttcgccgtat tacggcccaa gtgttcatca
     4381 ggaaagaaga gaattttttc tcgcctggcc cagcaccatt caatcacagc tttggcattg
     4441 gaagacgtgc aggtgattcc tccatgctca ccacaaaacg ccttgagcac cgcagcggag
     4501 tttacgtaga ccgccggcat caccgtgtct tccacgggaa ccacgcgtcc caatgcttcc
     4561 cagcactggt caacctgttc gatcgccgcc atatcggcca tcgaacaacc cgcagccatg
     4621 tccggcagaa tcactgtttg ttgagaacgg ctgaggatgt ccgccgtctc ggccataaag
     4681 tgcacgccac aaaaaacgat gtaagggcgc tcggagcgtt cggcggccaa ttttgccaac
     4741 aaaagcgagt caccgcgaaa atcggcgtgc tggatgactt cgtcccgctg gtaattgtgg
     4801 cccagaatca tcactcgctc accaagcgtg ctcttcgccg ctgccgtccg acggaataat
     4861 tcctccgtcg actgcgtctg gtagtcggtg attggtcttg atactgccgc agtaaccgtc
     4921 acaatatgcc tccaaccata aagaaattta ttctacgata gccttccctc acaatcaacg
     4981 aatagcccta tgaggaaaag tcctagtcgg tcgcccaaag gatggtaggc ggaatgggtc
     5041 taataaccga ttgacttgac tcatctccga ggaatacgat cagcgtcaat agctagagct
     5101 aggggggcct tgaggctgag agtccgaatg atcggacgac cctcacaacc tgacctgggt
     5161 aataccagcg tagggaagcc ggccgaaagc ggatctcgtg aggttcagcc gccttcccta
     5221 ccctgggaaa gcggcttttt tattgcagca tgccacgtca gcctttggct ccccatatcg
     5281 accaaccatg aaaggatctc ttatatgagc atccagacaa atacacatgg atcggttaac
     5341 gggaatggga tcccgacttc tccattgacc acgacgccct ttccggcatc gggcaaggtc
     5401 tatgtgaatg gtacccagtc aggggtgcgc gtcccgatgc gggaaattag tttgacccag
     5461 acgacggggg cgaatgggac gagatcaaac aatgctcccg ttatcgtcta tgatacctct
     5521 ggtccttata ctgatcccgc
//
LOCUS       DATNDD010000193         5132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2329154, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000193 DATNDD010000000
VERSION     DATNDD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329154"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VN647_04655"
     CDS             <1..297
                     /locus_tag="VN647_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWG96356.1"
                     /translation="GSKVVPVTLILSPSTTSPPPSTTSSATLTWNAVTGTVSGYKVYV
                     GEAPRLYTRTITVGNATSSTVNSLTVGRMYYFAVTAYNSAGESGPSNEVSKTIQ"
     gene            complement(987..2342)
                     /gene="prsR"
                     /locus_tag="VN647_04660"
     CDS             complement(987..2342)
                     /gene="prsR"
                     /locus_tag="VN647_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HWG96357.1"
                     /translation="MATGPALLLVDNDAVFREQMKWALTSDYDLMEASDRSAALSQIR
                     RDMPPLVLLDLGLSPDVVGTSEGLVILRETLRLNPVAKVIVVTSNSDRANAVAAVENG
                     AYDVIEKPVQLDVLKVVLQRATYLSNLDQESRAIQERAVMTEFEGLMGNSKPMQDVFG
                     IIRRVGPSDVPVLITGESGTGKELVARAIHRLSGRKEAPFVAINCGAIPETLLESELF
                     GYEKGAFTGAVQQRKGRIESAQGGTLFLDEIGDIPLSLQVKLLRFLQDHEIHRLGGRD
                     TVSVDVRILAATNVDLQEAIDHGRFREDLYYRLRVVAIPVPALKARGSDISLLARAFF
                     MKFAQAQKKVLKGFTSQAIEALLAHNWPGNVRELENRVKRAVVMAEGQYISPANLELK
                     ELSPIDGESSRLRVARDIREKELVRFALEKTDGNVSKAAVELGISRPTLYQLLSRYGL
                     R"
     gene            complement(2401..3456)
                     /locus_tag="VN647_04665"
     CDS             complement(2401..3456)
                     /locus_tag="VN647_04665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWG96358.1"
                     /translation="MAPVQGRVRTINPLLDERWSEFVDRHPNSSVFHTIGWLSALHTT
                     YGYEPVAFTTSAPSEKLTNALLFCVVRSWLTRGRLVSLPFTDHCEPLVENIEQLRALC
                     THLETLCRTQRWKYVEMRTGDAVFGAEEGLGECKVYRWHRLDLRSSLDALYKGFHKDC
                     IRRRIRHAERQGLRYEEGRNESLTRSFYDLIVLSRLRKHLPPQPFEWFQNLVACMGKD
                     VCIRIAFKGNQPVAGILTMRHRKKIYYKYGGSDARLNDLGATPMLFWQAIQAAKSAGM
                     EELDLGRSELENQGLIRFKERWGAHSVRLTMWRGPADVVSPSLERLKVRLARTICTCV
                     PRTMLISVGRLMYRHVG"
     gene            complement(3511..4332)
                     /locus_tag="VN647_04670"
     CDS             complement(3511..4332)
                     /locus_tag="VN647_04670"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HWG96359.1"
                     /translation="MAVVFTGSLHDEHSEHFSHIFLIPFLCLCLLYLRRAAIFATVEW
                     RPFLGSILMAVGGAISLGTKDCISETLDSCSLTMLSFVMMCWGTFLLCYGIHALRAAS
                     FGLGLMVFMVPFPSVVLDAVVAFLQHSSSEATDLLFSVLGIPVFREGFVFGLSDFTIY
                     VAEECSGIRSALALFITGLVASHLFLRSAWGTMGLLSIVVPLAIVKNAVRIVGLALLA
                     NYVDPAFITDSTLHRNGGIPLFVLSLTVLFSLVWLIRKVEQRFGYNKQNGSHAQV"
     gene            complement(4733..>5132)
                     /locus_tag="VN647_04675"
     CDS             complement(4733..>5132)
                     /locus_tag="VN647_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HWG96360.1"
                     /translation="AHLNTQGAHDMARLNAFVRSIAWYRLVPSGLDGMKTIVTAGGSQ
                     SVLADYVAAAASPDGTLLVAYVPPDHTGPITIDMTVMSGQTQARWFNPATAEYTLIGT
                     SLANTSTHAFVPPGNNGTGFNDWVLLLERQ"
BASE COUNT         1323 a         1455 c         1232 g         1122 t
ORIGIN      
        1 ggaagtaagg tagtcccggt gacattgatt ctctctccct ccactacctc gccgccgcca
       61 tcaaccacgt cctcggcaac tctcacgtgg aacgcggtca ccggcactgt cagcggctac
      121 aaagtgtacg tgggggaggc cccccgcctc tatacgcgaa ccattacggt aggcaacgcc
      181 acatcatcga ccgtgaacag tttgactgta ggccggatgt actatttcgc cgtcacggca
      241 tacaacagtg ccggtgagag tggtccttcc aacgaggtca gcaagaccat ccaatgaacc
      301 actagctgca tcagccccaa aaaataaagg gccatcccct acctggggat ggccctttta
      361 ctccgtgttc tctttataga aaagtctggg ctactctttc cgtgaagtcc gcttccagat
      421 cccaagccca gctaacccag cgcccagcag gatcaaggat gccggttcag gaacggatgc
      481 gcaccgccgg ccacaaggta ggggtatggg taggggctta atcctaataa ggctagtact
      541 atccgtagcg atgctaatgc tctcactcgt aagactgatg ctactggtcg tttgagccat
      601 cgcaagattc cctgagaagc cgagaataat tacgacgact ccaaaaagga gtaaatgaat
      661 caacccaccc tcattcttca tctttacctc tcccagttac aactgatcaa cgtcgcggtg
      721 ctacatcgtc acatgtggta aagcacatct tgttccagca gtacgattta ctataaacat
      781 ttgaaaatat tgcagctttg tatttgagtt tttgttcgca caatacaagc tgtaaaaaca
      841 atttacacca agggactgca gaagatttga atgagaactt atttcaagat tttcaacagg
      901 tcatgaaatt ctcaaactgt aaaataaatt tacacatcgg cagcttcttt acgctttcct
      961 cattttccag cttatcgata gtttcgctac cttaacccat atcgggagag caactgatag
     1021 agagtgggac ggctgatccc caattcaaca gccgccttgg acacattgcc atcggttttt
     1081 tccagcgcga acctcaccag ctccttctca cgaatgtcgc gtgcgactcg aagcctcgac
     1141 gattccccat cgataggcga gagttctttc aattccagat tcgcagggct gatgtattgc
     1201 ccttctgcca tcacaactgc ccgcttgacc ctattttcca actctcggac atttccaggc
     1261 caattgtgcg ccagtaaggc ttcgatcgct tgtgacgtaa accctttcag tacttttttt
     1321 tgtgcttgtg cgaacttcat gaagaacgct cgtgcgagga ggctgatatc agaacctcgg
     1381 gccttcaagg cagggacggg gatggccacc acacgaagcc tgtaatacaa atcttcccta
     1441 aatcgaccat ggtcgatagc ctcttgcaga tcaacgttcg tcgcagcaag aatcctgaca
     1501 tcgaccgaaa ccgtgtccct cccccccaat cgatggattt catgatcttg taaaaaccgc
     1561 agtaacttga cctgcagact gagcggaata tccccgattt catccaagaa gagtgtgccg
     1621 ccttgcgcag attcgatccg gccttttcgt tgctggacag ccccggtaaa cgctcccttc
     1681 tcatacccga acagctcgct ctccaacagt gtctcaggga tcgcgccgca gttgatagcg
     1741 acaaagggcg cctctttgcg tccgctcagt cggtgtattg cgcgtgccac aagttccttc
     1801 cccgtcccgc tctccccggt gatgaggacc ggaacgtcag acgggccgac acggcgtatt
     1861 atgccgaaca cgtcttgcat aggcttactg tttcccatga gaccttcaaa ttcggtcatg
     1921 acggcccgct cttgaatggc gcggctctcc tgatccaaat tcgagagata cgtcgcccgc
     1981 tgaagtacca ccttcaacac atcgagctgt accggcttct caataacgtc gtacgcgccg
     2041 ttttcgacag cagcgacagc attcgctcga tcgctgttgc tggtgacaac gatcaccttg
     2101 gccacaggat tcagtcgaag agtctcacgc agaatcacca atccttcgga agttccgact
     2161 acatcggggg acagtcccaa atccagcaac accagcggcg gcatgtcccg gcgaatttgc
     2221 gagagagcgg ctgagcgatc cgatgcctcc atcaggtcat agtctgaggt aagggcccat
     2281 ttcatctgct cccgaaaaac ggcatcattg tctacaagga gtaatgcagg accagtggcc
     2341 atgtcagaag ttctcctctg ttacaaacat acgcgctcgc caaatactag accacatcgc
     2401 tcagccgaca tgtcgataca tgagccgccc caccgatatc agcatggtgc gaggaacgca
     2461 ggtacaaatg gttctcgcca gacgcacctt tagacgctct aaagaagggg acacgacatc
     2521 ggcaggaccg cgccacatag tcaacctcac gctgtgtgca ccccaacgtt ctttgaatct
     2581 gatcagtcct tgattctcga gctccgaccg gcccaaatcg agctcttcca tcccggccga
     2641 ctttgcagct tggatggcct gccagaaaag catcggggtc gcccccagat cgttaagtcg
     2701 ggcatcggaa ccgccgtact tgtaatagat cttcttgcga tgcctcatgg tcaagattcc
     2761 tgcaacaggc tggttcccct tgaacgcaat gcgaatacac acgtcctttc ccatacaagc
     2821 aacgaggttc tggaaccatt cgaacggctg cggcggaaga tgcttacgca acctcgacag
     2881 cacgatgaga tcataaaatg accgcgtcag tgattcgttc cgaccctcct catacctcaa
     2941 tccttggcgt tccgcgtgac gaattctccg tctaatgcaa tccttatgga atcccttgta
     3001 taacgcatcg aggctggacc gaagatcgag tcgatgccat cgataaactt tacactcccc
     3061 caggccctcc tcggcaccga agacagcgtc gccggtccgc atttcgacat acttccatcg
     3121 ctgtgttcta cagagcgttt caagatgagt acacaacgcc cggagttgct cgatgttttc
     3181 gacaagcggt tcgcagtggt ccgtaaaggg cagcgaaacc agtcggccgc gcgtcagcca
     3241 acttcgcacg acacaaaaca ggagggcgtt cgtcagcttt tcagatggcg cagaggtcgt
     3301 aaaggcgacc ggttcatacc cataggtggt gtgcaacgca ctaagccagc cgatcgtatg
     3361 gaagacagaa gaattcggat gtctatcgac gaattcggac caccgctcat ctagaagagg
     3421 gtttatcgtc cgaacacgac cctgtactgg agccatctcg catcccgcca acatcaggtt
     3481 cgtccatagt gtgtcgctat gatgttctgg tcagacttgc gcatgagacc cattttgttt
     3541 gttatacccg aaccgttgtt ctacctttcg tatcagccag acaagagaaa agagcactgt
     3601 gagcgacaag acgaagagcg gaatgccgcc attccggtgg agagtgctgt ctgtaatgaa
     3661 ggcaggatcg acatagtttg caagcaaagc cagcccgacg atccgcaccg cgttcttcac
     3721 gatcgccaac ggaacaacaa tggagagaag tcccattgtc ccccaagcag atctgagaaa
     3781 caggtggctc gccacaaggc cggtaatgaa taacgcgaga gcagatcgga tgccgctgca
     3841 ttcttctgcc acatagatcg tgaagtccga taggccgaat acgaaccctt cgcgaaagac
     3901 aggaattccg agcacagaga atagtagatc ggttgcttcc gacgaactgt gctgcaggaa
     3961 ggcgacgacg gcatcgagaa caacggaagg aaacggaacc atgaaaacca taagacccaa
     4021 tccaaaggaa gccgcacgaa gggcatggat cccatagcag agtagaaagg tcccccagca
     4081 catcatcacg aaagaaagca ttgtgagaga gcaactgtcg agcgtctcgc ttatacagtc
     4141 tttcgttccc aacgatatcg cgcctccgac tgccatcaga atcgatccga ggaacggcct
     4201 ccattccacc gtggcaaaga tcgccgctcg acgaaggtac agcaagcaca gacaaagaaa
     4261 cgggatcaag aagatatggg aaaagtgctc ggaatgttca tcgtgcaggg aaccggtgaa
     4321 gacaactgcc aaggggcctc gaaaccaaac aatacatgca ataacaagaa agaggaaaca
     4381 gatatcccta cgctcgaaac acgcgagcca cggcgatcgc atccacggac tccaacctgt
     4441 tcgtggatgc tggttctccg aaggcactat actctgtacg tttacttctc tttgcggcat
     4501 cacttggagg aagctccgac tatttcaaac tacgttaaaa gcacgtccgc tgttttcgtt
     4561 cataacatgc agaggggtac cgaaccagat attaggcgca cacaaaagcc atgaaacatt
     4621 aagataaata gctaatgatt tttagaatca aagaccgatg cagatagcta ggcatcttcg
     4681 atgccgtaga aaggtggctg tcaaacgagt tttgaggtgc ccatcgctca ctttattgtc
     4741 tttccaagag taacacccag tcattaaagc ccgtgccgtt attcccgggc ggcacgaagg
     4801 catgcgtgct ggtgttagct aacgacgtac cgattaaagt atattcagct gtggccgggt
     4861 tgaaccagcg cgcctgtgtc tgaccactca tcaccgtcat atcgatggta atcggccctg
     4921 tatggtcagg cggtacgtaa gccaccagca aagtgccatc cggcgacgcc gctgcggcca
     4981 catagtctgc aagaaccgac tgcgatcctc cggcagtcac aatcgtcttc atcccatcga
     5041 gaccggatgg gacgaggcgg taccaagcaa tcgagcgaac aaaggcattg agccgcgcca
     5101 tatcatgcgc accctgtgta ttcagatgtg cc
//
LOCUS       DATNDD010000194         5376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3336236, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000194 DATNDD010000000
VERSION     DATNDD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3336236"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /gene="topA"
                     /locus_tag="VN647_04680"
     CDS             complement(<1..1279)
                     /gene="topA"
                     /locus_tag="VN647_04680"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWG96361.1"
                     /translation="MGKSLIIVESPTKARTITKYLGRGYTVMASVGHVKDLPTSKLGV
                     DLENDFEPQYVTIKGKSKVLAEIKKKAEEADKVFLAPDPDREGEAIAWHIEQELLGKS
                     KKKKKDGKIFRVLFNEITESAVKRALQSPGEIDMKLVNAQQARRVLDRIVGYQGSQLL
                     WNKVRRGLSMGRVQSVAMRLICEREAEREAFRSEEYWSITALLAGTNPPAFEAKLHSI
                     NGEEASIENAEQAGRIVGEIQGKAFVVHSIERREKKRNPVAPFITSRLQQDASRKLHF
                     SPKKTMTLAQQLYEGIEIGAEGATGLITYMRTDSPRISNEAMADAREVIQSRFGADYL
                     PATANVYKTSKAAQEAHEAIRPTSAGRDPESVRQYLDHDQYNLYKLIWNRFIASQMVP
                     AVLDVTRIDSAPVGTKENYLFRSTGTVVKFPGHT"
     gene            complement(1535..2653)
                     /gene="dprA"
                     /locus_tag="VN647_04685"
     CDS             complement(1535..2653)
                     /gene="dprA"
                     /locus_tag="VN647_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWG96362.1"
                     /translation="MDNASLTCWLTLQAIDGVGDRTLLKLIHAFGAPDAVLGATMDDL
                     IGAGCSPELAESVRRGPESSIRRQIDRQVKIVGRLKIQTVTLFDRSYPTRLKTIPDPP
                     PLLYVSGSLSPKDEVAVAIVGGRRATPTGRLITEEIAKDLAGCGVTIVSGLARGIDAA
                     AHRGALTGKGRTIAVLGCGIDRTYPSEHHMLRRNIESHGVVISELPIGAAPQSHHFPR
                     RNRIISGLSLGVLVGEAATDSGSLITAKLALEQGREVFAVPGSVKEEACRGSNRLIKE
                     GAKLVEGAQDILDEILPQVDARQRATLHFDRALTEVRVPLGKEEASVYETLSYEARSI
                     DTVIEHTGLSAAEVSAALLSLELNGRIRQLPGQQYIRL"
     gene            complement(2747..2893)
                     /locus_tag="VN647_04690"
     CDS             complement(2747..2893)
                     /locus_tag="VN647_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96363.1"
                     /translation="MGAHPTVAELLQDEERYGVEVLERDCSAKIIVTPDSQLHLEQYD
                     LVVF"
     gene            complement(2951..4255)
                     /locus_tag="VN647_04695"
     CDS             complement(2951..4255)
                     /locus_tag="VN647_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWG96364.1"
                     /translation="MGSEIAISVAREETRVAVLDGGVVTDLFGDRTKHKDFVGNIYKG
                     KVAKVLPGMQAAFVDIGLEKAAFMHVSDLLVDAEPGDMLVEAEEDEKDSEMLPPKHQS
                     AKPIEQLLSEGQELMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPNVDHIGVSRRIPR
                     DEERARLKEIMRRVRRSGFGYIVRTVSEGVKEDELKSDVDFLHVLWQDILTKREQMPA
                     PALLHSDLSLSFRVVRDLFGKKVDRLWIDSREEYEAIRDFVQRFSPEQTSRIHFYDKD
                     EPLFDHLGVEQEIARALSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETIL
                     RNNLEAAKEVAYQLKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMASDKARTRISR
                     ISDLGLIEISRERVREDLLRSLSEPCRYCDGRGYTKSPTTVA"
     gene            complement(4417..>5376)
                     /gene="rodA"
                     /locus_tag="VN647_04700"
     CDS             complement(4417..>5376)
                     /gene="rodA"
                     /locus_tag="VN647_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWG96365.1"
                     /translation="IGLGVAAFLVMWASDYHHLARFAYPAYAVILLMLVFVLFEGRTS
                     KGAQRWIALGPLNFQPSEFAKLILVLVLAHYYSKAPRVGWLQRVIVPGLLVLPGLLLI
                     LKQPDLGSGLSFVAVYAAMLLMVGVRSQALGFILLFSIMLFPFAWEGMWGSLHDYQRQ
                     RIMAFVDPDYDPAGKGYHALQSKIAIGSGELLGKGLYGGTQSQLKFLPEGHTDFVFAV
                     FAEEWGFLGVLVLLTLFVALIWLSLEIASKAKDQLGALLAVGIVAMLCFCVVVNIGMT
                     AGMFPIVGIPLPLVSYGGSATIMTMASLGLLLNVKRKRLSLFY"
BASE COUNT         1163 a         1690 c         1352 g         1171 t
ORIGIN      
        1 tcgtatgacc ggggaacttc accacagttc cagtggaacg gaagagatag ttttctttgg
       61 ttccaaccgg tgccgaatcg atacgggtaa cgtccaaaac ggccggcacc atctgcgatg
      121 cgatgaaccg attccagatc aacttataga gattgtactg atcgtgatcc aagtactggc
      181 ggaccgattc cggatcgcgt cccgctgagg tcggtctgat tgcttcatgc gcttcttggg
      241 ccgcctttga agtcttgtag acattcgccg tggcagggag atagtccgcc ccaaatcgag
      301 actggatcac ctcgcgcgcg tcggccatcg cctcattgga aatgcgcggg gaatcggttc
      361 tcatataggt gatgagaccg gtcgcgccct cggcgccgat ttcgatccct tcatacaact
      421 gctgcgccag agtcatcgtt ttcttcggtg agaagtgcag cttccgcgaa gcgtcctgtt
      481 gcagccgact cgtaatgaac ggcgcgacgg gattccgttt cttttctcgg cgttcgatcg
      541 agtgtacgac gaatgccttt ccttgaatct cgccgacgat gcggccggct tgctcggcgt
      601 tttcaatcga ggcctcttcg ccgttgatac tgtggagctt cgcttcgaat gccgggggat
      661 tggttcccgc cagcagcgcg gtgatcgacc agtactcttc gcttcggaac gcttcccgtt
      721 ccgcttcccg ctcgcaaatc agccgcatgg ccaccgactg cactcgcccc atgctgagcc
      781 cgcgccggac cttattccag agcaactgac tgccttgata ccccacgatg cgatccaaca
      841 cacggcgggc ctgctgggca ttcaccagct tcatatcgat ctcgcccggc gactggagcg
      901 cgcgcttaac ggctgattcc gtgatttcgt tgaagagaac tcggaagatc tttccgtctt
      961 tctttttctt cttcgatttt ccgagcagct cttgctcaat gtgccaggca atcgcctccc
     1021 cttcacgatc ggggtccggc gccaaaaaga ccttgtcggc ttcctcggcc ttcttcttga
     1081 tctcggccaa caccttggac ttgcccttga tggtgacgta ctgtggttcg aaatcgttct
     1141 cgagatccac acccaatttg ctggtcggaa gatccttgac atggcccacc gacgccatga
     1201 cggtataacc gcggcccaga tatttcgtaa ttgtcctcgc cttggtcggc gactcaacaa
     1261 taatcaacga ttttcccatg acaactccgt ctaacctctc aagaattgta tcatccgtac
     1321 caaatatcgg agattcgtgc aactagaatg gcagtcggca accatcagcg agaggttagg
     1381 aaccatagag gatgctaaga aagtgtaacg acgacaagac cttcctgcta tgcgcttcac
     1441 gtatcattcc actcgtcaca cgcgaagacg ttcctcacac cgacgagtcg tcgatgttca
     1501 ctcgatcgac gattgacgcg atgtcttgcc ggtgctagag gcggatatat tgttgcccag
     1561 gaagttggcg aatccgtccg ttcaattcca atgaaagcaa cgccgcggac acttccgcag
     1621 cgctcagccc ggtgtgctca atcaccgtat cgatggatcg ggcttcatag gacagcgttt
     1681 cgtacaccga tgcctcttct ttccctagcg gcacgcgcac ttctgtcaac gcgcggtcga
     1741 aatgcagcgt ggcccgctgc cgggcatcga cttgcggaag aatctcatcg aggatgtctt
     1801 gtgctccttc gaccaatttc gccccctcct tgatcaagcg attcgatccg cgacaggcct
     1861 cttccttgac ggaaccgggt acggcaaata cctcccggcc ttgctccaac gccagtttcg
     1921 cagtgatcaa cgagccgctg tcggttgccg cctcgccgac cagcacgccc aaggaaagtc
     1981 cgctgatgat ccgattcctt cgcgggaagt gatgactttg aggggcggca ccgatcggca
     2041 gttccgagat cacgacgccg tgtgattcaa tattccgccg cagcatgtga tgctccgagg
     2101 ggtaggtccg atcgatgccg caaccgagga ccgcgatcgt gcgcccctta cccgtcagcg
     2161 cccctcggtg cgccgcagca tcgattcccc gagccaagcc gctcacgatc gtcaccccac
     2221 atccggccag gtccttcgca atctcctccg tgatgagtct tccggtcgga gtcgcccgtc
     2281 gtccacccac aatcgccacc gcgacctcgt ccttcggtga taggctcccg ctcacataca
     2341 acaatggcgg cggatcggga atggttttga gtcgggtcgg ataggatcga tcaaacagcg
     2401 tgaccgtttg aatcttgagg cgtccaacaa tcttcacttg gcgatcgatt tgccgccgga
     2461 tgctcgactc aggcccgcgc cgcacggatt cggccaattc gggactgcaa ccggcgccga
     2521 tcaagtcgtc catcgttgcg ccgagcaccg catctggtgc tccaaacgca tggatcaact
     2581 tgagaagggt gcgatccccg acaccatcaa tcgcttggag cgtgagccag caggtcaagg
     2641 atgcattatc catgatcggt ctcgatcatc ggcagggact cgcttcaaca gcaggaaggt
     2701 ccgctaggca gaacatctcc tcgacaatga gcggcggccg tggcggttag aacacgacca
     2761 gatcgtactg ctccagatgc aactgactgt ccggtgtgac gatgattttt gccgaacagt
     2821 ctcgctccaa tacttccact ccgtaacgct cttcgtcttg cagcaattca gccaccgtcg
     2881 gatgggctcc cacgataatc cgttgctgat cggcagccgg ttcaattcgt cggatttcgc
     2941 ggaaaatttc ttacgccacg gtcgtgggag acttcgtata tccacggccg tcgcagtagc
     3001 ggcacggctc cgacagcgaa cgtaacaggt cctcccgcac ccgttcgcgg gagatttcga
     3061 tcaagccgag gtccgaaatc ctcgatatcc tcgttctcgc cttgtccgac gccatggcgt
     3121 ccaccaacgc atggtagacc ttatcccgat tcttctcccg ctccatgtcg atgaagtcga
     3181 caataatgat gccgccgatt cctcgcaact tcagctgata ggcgacctct ttggccgcct
     3241 cgaggttgtt gcgcaagatg gtttcttcct gatcccgttt ccccacaaaa cgtcccgtat
     3301 tgacgtcgat caccgtcatc gcttcggtat gatcgatcac gagatagccc cccgacttga
     3361 gccagacttt ccggctcaat gcacgtgcaa tttcctgctc cacccccaga tgatcgaaca
     3421 agggctcatc cttatcgtag aaatgaatcc gcgaggtctg ttcgggagaa aatcgctgca
     3481 cgaagtcccg aatggcttcg tattcctccc gcgaatcgat ccacagtcga tccacctttt
     3541 tgccgaacag gtccctcacg acacggaagc tcaagctcaa gtcagaatgc agcaacgcgg
     3601 gggcgggcat ttgctctcgc ttcgtcaaga tgtcctgcca gaggacgtgc agaaagtcga
     3661 cgtcggattt caactcatct tctttgacgc cttcactgac cgtccgcacg atatagccga
     3721 agccggaacg cctgacccgg cgcatgatct ccttcagcct ggctcgttct tcatcccgcg
     3781 gaatccgtcg agacacaccg atatgatcga cgttgggcat aaagacgaga tatcgccccg
     3841 gaagcgagac gtacgtcgtc actctggacc ccttggtgcc gatcggcccc ttcgaaatct
     3901 ggaccatcag ctcctggccc tcactcagca actgctcgat cggttttgcg ctctgatgct
     3961 ttggcgggag catttccgaa tctttttcat cttcctcggc ctccaccaac atgtctcccg
     4021 gctctgcatc caccagcaga tcggagacgt gcataaacgc cgctttttcc aagccgatgt
     4081 ccacaaatgc ggcttgcatg ccaggcaata ccttggccac ctttccttta tagatatttc
     4141 cgacaaaatc cttatgcttc gtgcggtcgc cgaacaaatc ggtgacgact ccgccgtcga
     4201 gcacggccac acgggtttcc tcccgcgcga cgcttattgc aatctcactt cccatattga
     4261 ttgactcctt ttgtcgcaag ccgcgtgagc cggctgagag atggacgcag gcccacaccc
     4321 aggtccgtca ctcactccgc acaccggctc ccccggcttt ccacactatc ttctccgaac
     4381 gatggaaccc aagccttccg tctgctcggt catcgctcaa taaaacaggc tcagccgctt
     4441 ccgtttgacg ttcaacagca agcccaacga cgccatcgtc ataatggtgg cgcttccgcc
     4501 atagctcacc agcggcaagg gaatgccgac gatcggaaac atccccgccg tcataccgat
     4561 attgaccacg acgcaaaaac acaacatcgc cacgatcccc acggcgagca gcgctccaag
     4621 ctgatccttc gccttagacg cgatttccag cgacaaccaa atcaacgcca caaacaacgt
     4681 caagagcacc agcacgccga gaaatcccca ttcttctgca aaaacggcga acacaaaatc
     4741 agtatggcct tccggcaaaa acttcagctg actttgggtg ccgccgtaga gccccttacc
     4801 caacagttcc cccgacccga tcgcgatttt cgattgcagg gcatgatagc cctttcctgc
     4861 cggatcgtaa tcggggtcga cgaacgccat aatgcgctgt cgctggtaat catgcaagga
     4921 cccccacatc ccctcccagg caaacggaaa tagcatgatc gagaacaata gaatgaatcc
     4981 tagggcttgc gaacgaactc ccaccatgag gagcatggcg gcatacaccg cgacaaagct
     5041 gagcccgctc cctaaatcgg gttgcttcaa aatgaggagg aggccgggca gcaccaagag
     5101 acctggaacg atgacgcgtt gcaaccagcc cacacgtggg gctttcgagt agtagtgggc
     5161 caataccaag acgaggatga gcttggcgaa ttcagacggc tggaagttga gcggacctaa
     5221 cgcgatccac cgctgagccc ctttgctcgt tcgcccttca aataacacaa aaaccagcat
     5281 cagcaagata acggcgtagg ctggataggc aaaccgagca agatgatgat agtccgatgc
     5341 ccacatgacg aggaaggccg ccacgccgag accgat
//
LOCUS       DATNDD010000195         4924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2883340, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000195 DATNDD010000000
VERSION     DATNDD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2883340"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2356)
                     /gene="treY"
                     /locus_tag="VN647_04705"
     CDS             complement(<1..2356)
                     /gene="treY"
                     /locus_tag="VN647_04705"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HWG96366.1"
                     /translation="MPGRRVLATYRFQLNCDFGFRRARDVTAYLHGLGITHCYSSSIL
                     AAMPGSMHGYDVIDPSRINPELGSEEEFKAWYCSLHDHDMGLILDIVPNHMGIAKALN
                     RWWYDVLENGPSSRYAGAFDIDWHPLKRELHNKVLLPILSDHYGVVLENQEITVGYEE
                     DRFVVHCADHGLPLTPQSWSTVLSYRLDALLCDDTAQAESEGLTELQSILTAIRHLPN
                     GEDSTPEGREEREREKEVIRKRIAVLMANNQRITAFVQDNMRLFNGTAGDPCSFDLLD
                     ELLNQQAYRLASWKVASEEINYRRFFDINELAAIRVEESSVFEATHEVVFKLIRDGVA
                     SGVRIDHVDGLYDPEGYLQRLQSWARRELSGDSSTERPLFVVVEKILGLDEPLPESWP
                     IEGTTGYAFLNTVNGLFVDPTRERAFSDLYRRITNRSQPYADMVYEAKQLIMRASMAS
                     EINVLGHQLNLLSERDRRSRDFTLNSLTNAIREIIACFPVYRTYVTVGAEPVSERDNA
                     FIHRAVAQAKRRNPTIGGEVFDFIRGLLLKERRLEADKTDEYERFVMKFQQTTSPVTA
                     KGIEDTVFYRYNRLLSLNEVGGEPQRFGIAPEVFHRRMVGRQARWSKGLSATATHDTK
                     RGEDARARLNVLSEIPDEWKARVVRWVKLNAKHRVKQESEEIPDANDEYLLYQSLLGA
                     WPLGDLRPEEREDFCTRIQQYMEKAIHEAKVHTSWINPNREYDDGVRRFVQTILDNSR
                     TNKFLDDFLPFKERVARAGLFNSLAQTLLKMTAPGTPDFYQGSEL"
     gene            complement(2371..3612)
                     /locus_tag="VN647_04710"
     CDS             complement(2371..3612)
                     /locus_tag="VN647_04710"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWG96367.1"
                     /translation="MRRLRVLGMIMAGGKGERLMPLTAVRSKPAVPFGGKYRIVDFVL
                     SNMLNSGITANYVLVQYRSQSLIEHVRESWSIAGRLKDQFITVVPPQMRAGGGWYEGT
                     ADAVYHNLNLIADFRPDVVAVFGADHIYRMDVSQMIQFHQDSGAEVTVAALPVPSEQS
                     SQFGILEVDADDRLVGFEEKPAKAKAMSGCPTMALSSMGNYIFDASVLLRTLERDAAS
                     PGSHDFGRNIIPDLIGGRRKVFAYDFLRNDVPGLRSYEERGYWRDVGTLDAYWQANMD
                     LLGETPSFDLRNNQWPVVSAGYESPMASIVRTYTDQSMIGQGSHIVDAEIRNSVIGRD
                     VHIEVGARLEGCIIMDGVRIGAQARLHRVIADRFSSIPAGSDIGFQESPQLPGCHLSP
                     TGLVIIPRDTKVAMTLMQSVS"
     gene            complement(3729..3875)
                     /locus_tag="VN647_04715"
     CDS             complement(3729..3875)
                     /locus_tag="VN647_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96368.1"
                     /translation="MEPGHFADPVLLQSQLNTLEVPLDDTLTVDTTRTPAEIVGIIRK
                     AFHI"
     gene            4055..4747
                     /locus_tag="VN647_04720"
     CDS             4055..4747
                     /locus_tag="VN647_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HWG96369.1"
                     /translation="MLLDGLLFERLHQATRLAHRRLERQVDIYRPNFNVHNYRRLLQD
                     FWGFYQPLERKLAVLADQNLPSSYSQRRRKAPRLKQDLLSLGVTETDIAALPLCGRLP
                     AIPTLPQVFGVLYVIDGAALGERIVVTHLGGDKLGISSSQGGSFLSGLSASYDPLLDT
                     WWQDFIAMTETMEDPNLQDLVIRSAMDTLECFETWLDHRKQSPLTNSMNSMRWSSQWE
                     FELFRTDVEKVA"
BASE COUNT         1071 a         1416 c         1311 g         1126 t
ORIGIN      
        1 acagttctga tccttgatag aaatcaggag tccccggagc cgtcatcttg aggagcgtct
       61 gcgcgagtga gttgaacagc ccggcgcggg cgactcgctc tttgaacggc aagaaatcgt
      121 cgagaaattt gttggtgcga ctgttgtcga ggatcgtctg aacgaagcgc cgcaccccgt
      181 cgtcatactc acgattcgga ttgatccagc ttgtatggac cttggcctcg tgtatggcct
      241 tctccatata ttgctgaatg cgggtgcaga agtcctcgcg ttcctcgggc cgcagatccc
      301 ccagcggcca cgcgcccaag agcgattgat agagtaggta ctcatcgttg gcatccggaa
      361 tttcctcgct ctcctgtttg acacgatgtt tggcgttcag cttcacccat ctgaccacgc
      421 gtgctttcca ttcatcgggt atctcggaca gcacattcaa tctggcacgc gcatcttctc
      481 cccgcttggt atcgtgggtc gcagtggccg aaagcccttt tgaccaccga gcttgtcggc
      541 cgaccatgcg gcgatgaaat acctccggag cgatgccgaa tcgttgtggc tctcctccta
      601 cttcgttgag agacaagaga cggttatatc ggtaaaacac ggtgtcttcg attcctttgg
      661 ccgtcacggg actcgtcgtc tgctgaaatt tcatgacaaa acgttcgtac tcgtcggttt
      721 tatccgcctc aagacgacgt tccttcaaaa ggaggccccg tataaagtcg aatacctcgc
      781 cgccgatggt aggatttctg cgcttggctt gggccaccgc ccgatggatg aacgcgttat
      841 cccgctcgct gacgggttcc gcccccaccg tcacgtatgt ccgatataca gggaaacaag
      901 cgatgatttc tcgaatggca ttggtcagac tattcagcgt aaagtctctg gatcgtcgat
      961 ctctctcgga cagaagattg agctgatggc cgagcacgtt gatctcgctc gccatggagg
     1021 cccgcatgat aagttgtttt gcttcgtaga ccatatcggc atagggctgg gaacggttgg
     1081 ttatgcgccg atagagatca ctgaaggctc gctcccgcgt agggtctacg aataatccgt
     1141 ttactgtatt gagaaacgca tatccagtgg tcccctcgat aggccaggat tcgggcagcg
     1201 gttcatcgag tccgaggatt ttttccacca cgacaaacag cggccgctct gtcgacgagt
     1261 cgccgctcaa ctcgcgcctt gcccaactct gcagtcgctg aagataccct tcgggatcgt
     1321 acagcccatc gacatggtcg attctcactc ccgacgccac accgtctcga atgagtttaa
     1381 aaactacctc atgggtcgcc tcgaacaccg aactctcttc cacacgaatc gcggcaagtt
     1441 cattaatatc aaaaaatcgt cggtagttga tctcctccga ggcgaccttc cacgatgcca
     1501 accggtaggc ctgttgattg agcaactcgt ccagcagatc gaaactgcat gggtctccgg
     1561 cggtaccgtt aaagaggcgc atattgtctt gaacgaatgc cgtaattcgt tgattgttcg
     1621 ccatgagcac ggcgatacgt ttgcggatga cctctttctc tctttcgcgc tcctctcgcc
     1681 cctccggagt gctatcctcg ccattgggaa gatggcgtat ggccgtcaaa atgctttgca
     1741 gttccgtgag tccttcactt tcggcctgcg ccgtgtcgtc gcacaacagc gcatcaagtc
     1801 ggtacgataa gacggtgctc cacgattgcg gagtgagcgg aagcccgtgg tccgcacaat
     1861 ggaccacaaa tcggtcctct tcgtagccga ccgtaatttc ctgattttcc agcacgacgc
     1921 cgtagtgatc cgataggatc gggagcaaca ccttgttgtg caactcccgc ttcagaggat
     1981 gccagtcgat atcgaatgct cctgcataac gggatgacgg gccgttttcc agcacgtcgt
     2041 accaccaccg attcagcgct ttcgcaatcc ccatgtgatt gggaacgata tccaggatca
     2101 gccccatgtc gtggtcatgc aaactgcagt accacgcctt gaattcctcc tctgatccga
     2161 gttccgggtt tatacgcgag ggatcgatga catcgtagcc gtgcatgctg cccggcatcg
     2221 cggccaggat ggatgaactg taacaatgcg tgattccgag gccgtgcaga taggcggtca
     2281 cgtcgcgcgc gcggcggaac ccgaaatcgc agttgagttg aaaccgatat gtggccagca
     2341 cacgacgtcc gggcatcacg actccatagc tcaggagacg gattgcatca gagtcatcgc
     2401 cactttggtg tcgcgcggaa tgatgaccag gcctgtcggc gagagatggc atccgggaag
     2461 ttgaggcgat tcctggaacc cgatgtcgct cccggcggga atactgctga agcggtcggc
     2521 gatcacgcga tgcagccgcg cctgagcccc gatgcgcaca ccgtccatga tgatgcaccc
     2581 ctcaagacgc gcgccgacct cgatgtggac gtcgcgcccg attacggaat ttctgatttc
     2641 cgcatccacg atatgactgc cttgaccgat catggattga tccgtgtagg ttcgcacgat
     2701 ggacgccatg gggctctcgt atccggccga gacgaccggc cactgattgt tccggagatc
     2761 gaacgacggc gtctcgccca acagatccat gttcgcctgc cagtaggcgt ccagtgtgcc
     2821 gacatccctc cagtatcccc gttcttcgta cgaccgcagg cccggcacat cgttccgcag
     2881 aaaatcatac gcgaatacct ttcttctccc gccgatgagg tccggaatga tattgcggcc
     2941 gaaatcgtgg ctgcccggac tggcggcatc ccgttccaat gtgcgcaaca acacggaggc
     3001 gtcgaagata tagttcccca tggaggacag agccatcgtc ggacatcccg acatggcctt
     3061 tgcctttgcg ggcttttctt cgaatccgac cagtcgatca tcggcatcga cttcgagaat
     3121 accgaactgg gacgattgct cgctcggcac gggcaatgcc gccaccgtca cttcagctcc
     3181 gctgtcctga tgaaattgga tcatttgcga aacatccatt cgatagatat ggtcggctcc
     3241 aaagaccgcc acaacatcgg ggcgaaagtc cgcaatcaaa ttgagattgt gatagaccgc
     3301 atcagcggtc ccctcgtacc atcccccgcc cgcccgcatc tgagggggaa cgaccgtaat
     3361 gaactgatct ttgagtctgc ctgcaatgct ccacgattcc cgtacatgtt cgatcaacga
     3421 ttgcgatcgg tattgcacca acacatagtt ggcggtgatg ccggagttga gcatgttgct
     3481 gaggacgaaa tcgacgatgc gatacttgcc gccgaacggc acggccggct tgctgcgcac
     3541 cgctgtgagc ggcatgaggc gctcgccttt tcctcccgcc ataatcatgc ccagaacccg
     3601 cagacgtctc atgatgccaa tgtatgttcc agacaaccct ttcgatactc gggaaatccc
     3661 tcgccgccga gatggcgagg cagcagtgtc ccgcgagaac gccgctggcg gactttttca
     3721 gcatcctatt agatgtggaa tgccttacgg ataatcccga caatttcggc aggagtgcgc
     3781 gtcgtatcga cggtcagagt gtcgtcgaga ggtacttcca atgtatttaa ttgactttgg
     3841 agcaagaccg gatcggcgaa gtggcccggt tccatggacg cacaccaccg agcatggtcc
     3901 caactctgct cgatgtgaga ctcaacagtt caacagtatt ggaagtctgc gcctcttcag
     3961 atctagggat ctaccgaggt ctgttcaaag gtgaaaatcc atagagtaag ctacctttta
     4021 taaaaagtgc catcattctg tggcggagga taccatgctg ctggacggcc ttctgtttga
     4081 aagattgcac caggctaccc gcttggccca tcggcgactt gaacgtcaag tggatatcta
     4141 tcgtccgaac ttcaatgtcc acaattatcg acggctcctc caagactttt gggggtttta
     4201 tcaaccgctg gagcgtaaac tcgccgtcct cgcggatcag aaccttcctt catcgtattc
     4261 acaacgacgg cgcaaggccc cccggttgaa gcaagacctg ctgtcgttgg gtgtaaccga
     4321 gactgacatt gcggcgctgc cgctttgcgg acgtctgccg gcgattccaa cccttccaca
     4381 ggttttcggc gtactgtacg tcatcgatgg ggctgccctg ggtgaacgaa tcgtggtcac
     4441 acatttagga ggagacaaac tagggatcag ttcatcgcaa gggggatcat tcctctccgg
     4501 tttaagcgca tcctatgacc ctctcctcga tacttggtgg caagacttca tcgctatgac
     4561 tgagacgatg gaagatccga acctccagga cttggttatc cgatcagcca tggatacctt
     4621 agagtgcttc gagacatggc tcgaccaccg aaaacaatct ccccttacga actctatgaa
     4681 ttcgatgcga tggagttccc aatgggaatt cgaactattc cgtactgatg tcgaaaaagt
     4741 ggcgtaatcg ccgccatcga acgatcgaca gacaccctca ggagacggtt cctacgacgc
     4801 tgaataccgg atcgtgcggc ccgatcgccg ggaaatgtgg gttagaaggg ataggccgtg
     4861 aacctcaaga ccatggactt tgtgcaatct caggcacagt tgagggcact ggccaacgag
     4921 ctca
//
LOCUS       DATNDD010000196         8268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1631527, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000196 DATNDD010000000
VERSION     DATNDD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631527"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..1152
                     /locus_tag="VN647_04725"
     CDS             481..1152
                     /locus_tag="VN647_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWG96370.1"
                     /translation="MHTNKSLITGTFVAGLSFAALTELPPAFAEMYVAGTAGVNFADR
                     INSIAGTGASVGVPDLNPAPNPDFDLQNSIVYGAKVGYFPGHRWYGVEGEVLHTTPHI
                     KSLDPEPGIHNSGIHMRVTTVGANFIARYPGRTFQPYAGAGIGAAIAHIGDTATVRSD
                     SDVAAAWNVLAGLRAFITPKIAIFGEYKYVGATLKFDQALGDLGGFSGNYRAQYVLGG
                     LSYHF"
     gene            1369..2067
                     /locus_tag="VN647_04730"
     CDS             1369..2067
                     /locus_tag="VN647_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWG96371.1"
                     /translation="MSRMIALFSLLCGLLILSACAESIHQVQRTAESLGIPLELENEI
                     DRTVRFSDLQQAPSEYVGRTVMLGGTIIKGKRTKEGTELEVLQRPTGEEGRLTEDRLR
                     SEGRFLAVRETFLDPASLPEGTPITVIGTVKGMTPRQLDESDYVYPVLEVKHIIDWKS
                     LEVRTRDRTLYYGSYYPPYGGAGFYPYGAFPYSYWGGPGFYRPYYFGPRSFSPGPSSP
                     PPPPPSVHPRLRGR"
     gene            2116..2850
                     /locus_tag="VN647_04735"
     CDS             2116..2850
                     /locus_tag="VN647_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HWG96372.1"
                     /translation="MKEKSEQSGYGLSRREALVLMGTTGAGLLVGAWPNKAQAVPGTP
                     RSLCIVRPEQTEGPYFVDERLHRSDIRADPAIGQVRPGAPLALTFQVMRLYAGECRPI
                     SDAQVDVWQCDAEGVYSDVEDPGFNTLGQKFLRGYQVTNAQGEARFLTIYPGWYPIRT
                     VHIHFKVRTTAIAGKRFEFTSQLYFPDELTDRVHTDLPYSLKGKRRVRNHHDFIFRNG
                     GEQLLLHPFTTNGSYAATFPIGLTIP"
     gene            2847..3032
                     /locus_tag="VN647_04740"
     CDS             2847..3032
                     /locus_tag="VN647_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96373.1"
                     /translation="MISTFRIPLIDYPHQISTPLVPPINYPKPLLISVQPHPGPIPPV
                     LTLNILRAMLSDVPEVL"
     gene            3156..3518
                     /locus_tag="VN647_04745"
     CDS             3156..3518
                     /locus_tag="VN647_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96374.1"
                     /translation="MKTSAIMLFAAVAQLIGSLAFANPAMLPKHPGYPMGKAVDPVTG
                     QSLANDPGQANAGGREALNNAALVDDSRSKQDLLFNQNDERLLEKPGAGLLPKVQGPN
                     ITIEPPVKEGTNVSAAPE"
     gene            complement(3634..4698)
                     /locus_tag="VN647_04750"
     CDS             complement(3634..4698)
                     /locus_tag="VN647_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWG96375.1"
                     /translation="MLISVASGKGGVGKSVVSANLALALAKGGRQVILADLDVGGADA
                     HIMFGELSPSVTLTDFLDKRVSRLDEVAIPVIIHPNLRLIAGTGDTLATANMAYARKK
                     RLMKQFRDLATDIVVIDIGAGTNLHALDFFLMADIHLAVATPEPTSVLDLYRFIKLAA
                     IRRVLACVLARSPLSEVLSNRDFTSVAEVMEIAGADDAGGRDAAAALQSFQPGLIINR
                     VSDSPQVNVLYLRKILHQYVGGDLTLLGQIPDDPAVSQAVRKFLPVIESAPESPAAKG
                     LLAVSAAVEQLISASADRSKEEAKQPEEKQTRVLCSNPACSQAPQASTSLKPDVPPPS
                     VGPRPPGCTNSAQNTSGLVA"
     gene            4939..6393
                     /gene="pyk"
                     /locus_tag="VN647_04755"
     CDS             4939..6393
                     /gene="pyk"
                     /locus_tag="VN647_04755"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWG96376.1"
                     /translation="MSMRKAKIVCTIGPASSSPDVLNRLIESGMDAARLNFSHGTHES
                     HAAAITAIRKAAAQCGSRVAIIQDLQGPRIRVGLLPKEGIKVNPGQVVRLRTSAQSGE
                     PHGVQASVPAPVPEIPVTYASLTRDLHRGSRVLINDGLIELQSVRIEDDLIECSVIIG
                     GMITSHKGINLPGTQVSAPTLTEKDREDIRFGAEHGVDYVALSFVRGPQDISAARAVL
                     TEHEINTPIIAKIERAEAVAALDEILEQADGVMIARGDLGVEMGPEAVPLLQKKIIVE
                     ANRRRRLVITATQMLESMTQAIRPTRAEASDVANAVFDGSDALMLSAETAVGAHPIEA
                     LQVMDRIIRAAEEGAEPGVVLKRQADLEHLSISESTCAAASFAARATDATAIVAFSES
                     GTTVRLISKQRPTAPILAFTHTEPIKRRMALYWGVRSFIMLRIGETDPHIREAEQQLK
                     AEGLVNKGDKVVIVSGTMAAQIGGTNMLKLHEIG"
     gene            6835..>8268
                     /locus_tag="VN647_04760"
     CDS             6835..>8268
                     /locus_tag="VN647_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWG96377.1"
                     /translation="MGHYKIGLIIPHPEDQKWDSVWKLFRTPNLNLPTLAALIPEDEW
                     DIEIQDEIVGPLDFTRDYDLVFITVTTVVAVRAYEVARLFRERGAKVVLGGIHPSTMP
                     DEAITHADAVVIGEGELTVPRLLEDFKKGKMQPQYRMPYMVEKWDEKPPRWDLLPQGY
                     MFRDALTATRGCNYRCTFCSIHLALGGGQYGFRKKPPADVVKLVEKMNGPMVMFWDDD
                     LLSDPAYTRELCQAMKPLGKKWMSQMSATYVAHHPELLKTLKEAGCSAMFMGIESINQ
                     DSLKSVDKQNQAKLYEEMIKRIHDQGIDIHAGFICGLDHEDVFDFERTAEWATKMGIA
                     GALWRIMTPYPGTKQFAELKAAGRILTENWTAYTGEHVVYKPAKMTVEQLYWGHKWAK
                     GQFYSYKSIGQRAIQRASQNGFGELLNITGCGLGYRSMFHLAADSAPVNVYRDMNHLP
                     PQSEPIAYRFPYPPKKRFSFVTDRLDQL"
BASE COUNT         1906 a         2278 c         2248 g         1836 t
ORIGIN      
        1 tcacgatgtc gcctagaccg tttttctgaa aggcccggat gagaccgttc agtccaccga
       61 tgccgctgtc cgttccgagg aggctgctca aggcctggag aagaggattc tgaccacctt
      121 ggcctcccat catgccacct accgcttgac tcaactgatc catgagtccc ataggtcggc
      181 tccttccctc acgtaaccgg catttttgaa aacgctctca aaacgacttc tatcgcgtga
      241 ggatgtagca cagacaccgg cggcaatcca aggggacccg gccggtggaa cgccgccatc
      301 tgcaaaaacc ctccattggt tatcctcaag agttcacaca tagcgaaaca tttggcgtca
      361 tcagtatgta aggaaacaga atatatcgca gcccattcat cgtcgcccga tgccgctcga
      421 tttttgcgag gcgttgtatc gaccaaactt ttggtatgat gtctcgaagg agtcacaggt
      481 atgcacacga acaaatcatt gatcaccggc acgtttgtcg cagggctttc attcgcagca
      541 ttgaccgagc ttcctcctgc tttcgccgaa atgtatgtcg ccggaactgc aggggtgaac
      601 tttgcggatc gcatcaacag catcgcggga accggcgcct ctgtaggagt tccagacctc
      661 aatcctgccc ccaatcctga ctttgacctt caaaattcaa tagtctatgg agctaaggtc
      721 ggatattttc ctggacaccg ttggtacgga gttgaaggag aagttctaca tacaactccc
      781 catatcaaat ccctagaccc tgagccagga atacataact caggaatcca tatgagggta
      841 accactgttg gagctaattt tattgctcgg tacccaggtc gcacgttcca gccctacgcg
      901 ggggccggca tcggagcggc aatcgcccac atcggcgata ctgcgaccgt gcggagcgac
      961 agtgatgtag cagcggcgtg gaatgtgctg gccggtctcc gcgcgtttat cacacccaag
     1021 atcgcgatct ttggagagta taagtacgtt ggtgcgacct tgaaattcga tcaagcctta
     1081 ggtgacctgg gtggtttcag cggcaattac agagcacagt atgtccttgg cgggttgtcc
     1141 tatcacttct aacatgacgc tgaaaaagtc cgccggcggc gttctcgcgt cgctcagagg
     1201 ctcaacgtac ccaagcgtac gcctcgcctc ttcgctcgct gcggccttgc tggacagcct
     1261 ttttgagcat cctgaagtta ttctgacatt agcaccgtac gcagaattcc atccatattg
     1321 ttggcatcaa ctgggttttt cgcagtctgc taggaaagga ggacaaccat gtcccgcatg
     1381 attgcgctct tttccctgct gtgtggattg ctgatcttgt ctgcctgcgc tgaatctatc
     1441 caccaggttc aacgaactgc ggagtcgctc ggtataccgc ttgaattaga gaacgagatc
     1501 gataggacgg tacgtttttc agatttgcaa caagctccta gtgaatatgt cggacgaacc
     1561 gtcatgctcg gcggcacgat catcaaaggg aaacgaacaa aggagggcac cgaactggaa
     1621 gttctgcaac ggccgaccgg ggaagaaggc agattgacgg aagatcgtct tcggtcagaa
     1681 gggcggttct tggcggtccg agaaacgttt ctcgaccccg ccagtctccc tgaaggcacg
     1741 cccattacgg tcatcggcac cgtgaaaggc atgacgccga gacagctgga tgaaagcgac
     1801 tatgtctatc cggtcctcga agtgaagcat attatcgact ggaagagtct cgaggttcgg
     1861 accagagaca ggaccttgta ctatggttcc tactatccac cctatggcgg cgcagggttc
     1921 tacccttatg gtgcctttcc ttacagttat tggggtggac caggtttcta tcgaccctat
     1981 tatttcggtc ccaggtcatt ttctcccgga ccatcatctc cgccgccgcc ccctccaagc
     2041 gttcatccac gactcagggg cagataatgt ccgatggctc gactactttc acaagggggg
     2101 aaaaggccta accgaatgaa agagaaaagt gagcaaagtg gatacggtct atctcgccgt
     2161 gaagcccttg tgttgatggg gaccactggt gccggtttgc tggtaggtgc atggccaaac
     2221 aaagcacagg ctgttcccgg cacacctcga tctctctgca ttgtccgacc agaacaaacc
     2281 gagggtccgt attttgtgga tgaacgattg catcggtccg acattcgcgc tgacccggct
     2341 attgggcaag tgcggcctgg tgcgccgttg gcgttgacgt ttcaagtaat gcgcctctat
     2401 gctggagaat gtcgacccat atcagacgcg caagtcgatg tgtggcagtg cgatgccgag
     2461 ggtgtgtatt ctgatgtgga agatccaggt ttcaacacac ttggccaaaa gtttttgcgc
     2521 gggtaccagg tgacgaatgc gcagggcgaa gcacggtttc tcacaattta tccaggttgg
     2581 tatccgatca gaaccgtgca tatccacttt aaggttcgca cgacggcgat tgccggaaaa
     2641 cgcttcgagt ttacatcgca attgtacttt cccgacgaat tgacggaccg cgttcatacc
     2701 gatcttccct actcgttgaa aggaaagcgt cgagtacgaa atcaccacga tttcattttc
     2761 cgtaacggcg gtgaacaatt actgctgcat cctttcacga cgaatggtag ctatgcggcg
     2821 acctttccaa tcggcctaac gattccttga tctctacctt ccggattcca ctcattgact
     2881 acccgcatca aatctcgact cccctcgttc ctcccatcaa ctatcccaag ccgctgttga
     2941 tttctgtaca gccgcatcct gggccgattc cccctgtctt gactctcaac attctgagag
     3001 cgatgctaag cgatgtcccg gaggtcttat gaaaccgggg tcgacacttc cacattttct
     3061 tacgttcatt gcctgcccat gccctgacgc tctccgaggg ccttgtgcct gcgagggcgt
     3121 tgttgcctct tccaccttag ccaggaggtt tcgccatgaa aacctcggcc attatgctgt
     3181 ttgctgctgt cgcgcagcta ataggttctc tcgccttcgc taatccggcc atgttaccca
     3241 aacatcccgg ctacccaatg gggaaagcgg ttgatcccgt gacaggccaa tcactggcaa
     3301 atgatccggg acaagccaat gccggaggcc gagaggccct caacaatgcg gcactcgtcg
     3361 acgacagccg ttccaaacaa gacttgctct ttaaccaaaa cgatgaacgc ctcttggaaa
     3421 aacctggagc aggactgctt ccaaaggtgc aaggtccgaa catcacgatt gaaccaccgg
     3481 tgaaagaagg gacaaatgtg agtgccgcac cggaatagtg aagattgttt cgagctgacg
     3541 gccggacatc cctgagaggt aaacggtgaa caccgccatg gagtgagggg tggagtacga
     3601 cgatacctct acaccgttcg caccttcact gccctaggca acgagcccgc tggtattttg
     3661 ggctgaattg gtgcaaccag gcggtctcgg tccaactgaa ggaggcggaa catccggttt
     3721 cagagaagtc gatgcctgag gggcttggga acatgctgga ttggaacaga gcactcttgt
     3781 ctgcttctct tccggttgtt tggcctcctc tttgcttctg tcggcagacg ccgagatgag
     3841 ttgctcgacg gcggcggaga cggctaacag acctttggcg gcgggggatt ccggagcaga
     3901 ttcgataacc gggagaaact tccgaaccgc ctggctcaca gctgggtcgt cagggatctg
     3961 accgagcagc gtcaggtccc caccgacgta ttgatggaga atcttgcgaa ggtacaggac
     4021 attaacttga ggactgtcgg atacccgatt gatgattaac cctggctgaa acgattgcaa
     4081 cgccgccgcc gcgtcacgcc cccctgcgtc gtcggcaccc gcaatttcca tgacctccgc
     4141 cacactcgtg aagtctcggt tggagaggac ttccgacagg gggcttcgcg cgagcacgca
     4201 ggccaataca cggcggatcg cggcgagctt gatgaatcgg taaagatcca gcacggacgt
     4261 cggctcggga gtagccacgg ccagatgaat gtcggccatc aggaaaaagt cgagggcgtg
     4321 caagttggtg ccggctccga tgtcgatcac cacgatgtcg gtcgcaagat cccggaattg
     4381 tttcatcagc cgcttcttgc gggcataggc catgttggcc gtcgccaagg tatcgccggt
     4441 gccggcaatg agacggaggt tcggatgaat gatgacggga atagcgactt cgtccagccg
     4501 gctgacccgt ttgtctagaa aatccgtcaa ggtcaccgac ggactcaatt ctccgaacat
     4561 gatgtgggca tcggctccgc ctacgtcgag gtcggccagt atgacctgtc tcccaccctt
     4621 cgccagtgcc aacgcaaggt tggctgaaac cacgctcttg cccactccgc ccttgccgga
     4681 tgccactgaa atgagcattc ccactgcgtc ttcctttcgt tactgaagtc attgtaccgg
     4741 ctgaaggaca aaagaaaagg aaatgcacga ttccgagcgg ccaaatcgcc gttcgcttac
     4801 gggtgaacac cggttcaaga tacactcaag tcacaacgcc tctctcgaca caaatccgtc
     4861 gtctatgagg tatccgatga atggatctat gatattctct tcctctcttc caactgacca
     4921 gcgtggaggc gcttccacat gagcatgcga aaggccaaaa tcgtctgcac catcgggccg
     4981 gcgagtagtt cgccggacgt gctaaaccga ttgattgaga gcggcatgga tgccgctcgg
     5041 ctgaactttt ctcacggcac tcatgagtct catgcggcag ccatcaccgc tatccgaaag
     5101 gcagccgcac agtgtggatc aagggtggcg attatccagg atttacaagg ccccagaatt
     5161 cgtgtgggcc tgctgccgaa ggaagggatc aaagtgaacc ccggccaggt ggtgcggcta
     5221 cgaacttcgg ctcagtcggg tgaaccacat ggcgtccaag cctccgttcc agcccctgtc
     5281 cctgagatcc cggtcaccta tgcttcactc acccgcgatc tccatagagg atcaagagtg
     5341 ctcatcaacg atggactcat cgagctacaa tccgttcgca ttgaagacga tttgatcgaa
     5401 tgttccgtca taatcggcgg catgatcacc tcgcacaagg gcattaattt gccgggcact
     5461 caagtcagtg cccctactct gaccgagaaa gatcgtgagg acattcgatt cggagccgag
     5521 cacggtgtgg attatgtcgc actctccttt gtacggggcc ctcaggatat cagcgcggcg
     5581 cgggctgtgt tgacggagca tgaaatcaac acgccgatca ttgcgaagat cgaacgggcc
     5641 gaagccgtgg ctgcgttgga tgagatcctg gagcaggcgg acggtgtgat gatcgcccga
     5701 ggagatctgg gtgtagaaat gggaccggaa gccgtgccgc ttctgcagaa gaagatcatc
     5761 gtagaagcca atcgccgccg ccgcctggtc atcaccgcca ctcagatgct ggaatcgatg
     5821 acacaggcca tccgtccgac gagagcagaa gcgtcggacg tggccaacgc cgtcttcgac
     5881 ggcagtgacg ccctgatgct ctcggccgaa acagccgtcg gggctcatcc gatcgaagcg
     5941 cttcaagtca tggatcggat tattcgcgcg gcggaagaag gggccgagcc gggggtcgta
     6001 ctcaaacgac aagccgactt ggaacatctt tcgatctcgg aatccacctg tgccgccgcc
     6061 tcttttgcgg cgcgggcaac cgatgcgacc gcaatcgtgg cgttcagcga aagcgggact
     6121 acggttcggc tgatttccaa gcaacgaccg accgccccga ttctggcatt cactcatacc
     6181 gagcctatta aacggcgcat ggcgctttat tggggagtcc gttcgttcat catgttgcgt
     6241 atcggggaga ccgatccgca catccgtgag gccgaacaac aactcaaagc tgaaggactg
     6301 gtcaataaag gggataaggt tgtgatcgta tctgggacga tggccgcaca aataggcggc
     6361 acgaacatgc tgaagctcca tgagatcggt taatgccgct caaacgtttt gcgtatattt
     6421 ctcccatctt cgtcatgact cttgattctg ttggcttgaa cgggctagtc tggcacccac
     6481 tgacccagtt ggtgtgcgat gagactccta ctcgccgaca ttttcaccct tcttccacac
     6541 atcgcacgag tcgagtcatc ggactgtcag aacgccgaag aaccagaatc aagcttgcga
     6601 tcagtcacac cgggccggac agttcacata gcttgatcga atgaagccct tatccaatcc
     6661 atacagttcc gcctcgccct caaattttga atcgaccacg gcttcccgct atagtactag
     6721 ggaaattgcc aggtagtgga catacgactg agtgatacaa gcaagcggac ggatggacca
     6781 tcaagaagac tttttaaaat agaagcattc attcaacact tggagggggc aactatgggg
     6841 cattacaaga tcggtctcat tattccacat cctgaagatc agaaatggga ttcggtctgg
     6901 aagttgttcc gtacaccgaa tttgaacctc cctaccctcg ccgctttgat cccagaggat
     6961 gagtgggaca tcgagattca agacgagatc gtcggccctc tcgacttcac ccgtgattat
     7021 gatttggtct tcatcaccgt caccaccgtc gtggctgtac gggcttacga agtggctcgt
     7081 ctcttccgtg aacgtggcgc caaggtcgta cttggcggca ttcatccctc gaccatgcct
     7141 gatgaagcca tcacacacgc ggatgcggtc gttattgggg aaggcgagct gactgttcca
     7201 cggctgctcg aggatttcaa gaagggcaaa atgcagccgc agtatcgcat gccgtacatg
     7261 gtggagaagt gggatgagaa gccgccgcgc tgggatctgt taccacaggg ttatatgttt
     7321 cgagacgcgc tgacggcgac aagagggtgt aattatcgct gcacattctg ttcgattcat
     7381 ttggccttag gaggcggcca gtacgggttt cggaaaaaac caccggccga tgtcgtcaag
     7441 ctcgtcgaga aaatgaacgg gccgatggtg atgttctggg acgacgactt gttgtcggac
     7501 ccggcctata cacgagagtt gtgccaagcc atgaagccgc tcggcaagaa atggatgagc
     7561 cagatgagcg ccacctacgt cgcgcaccat ccggaattgc tcaagacatt gaaagaagcc
     7621 ggctgttcgg caatgttcat gggtatcgaa tcgatcaatc aagattcgct taagtccgtc
     7681 gacaaacaaa atcaggcgaa gctgtatgag gagatgatca agcgcattca cgaccaagga
     7741 atcgatattc atgcaggatt tatctgcggc ttggaccatg aagatgtttt tgacttcgaa
     7801 cgcactgccg aatgggcgac gaagatgggc atagcaggag cactctggcg cattatgacc
     7861 ccctatcccg gcaccaagca gtttgcggaa ctcaaggcag ccggacggat cctcacggag
     7921 aattggaccg cctataccgg cgagcacgtc gtgtataagc cggccaagat gaccgtcgaa
     7981 cagttgtatt ggggacacaa gtgggccaag gggcaatttt attcgtataa gtcgatcgga
     8041 caacgggcga tccagcgggc ttcgcagaat gggttcggtg agctgctcaa catcaccggc
     8101 tgtgggctgg gctaccgctc catgtttcac ttggcggcag attcggcgcc ggtcaatgtc
     8161 tatcgcgaca tgaaccactt gccaccgcaa tcggagccga tcgcctatcg gttcccctat
     8221 ccgccgaaga aacggttcag ctttgtcact gatcgattgg accagctc
//
LOCUS       DATNDD010000197         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1182991, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000197 DATNDD010000000
VERSION     DATNDD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182991"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VN647_04765"
     CDS             complement(<1..297)
                     /locus_tag="VN647_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG96378.1"
                     /translation="MKQSEGDESLQPTLNRPIDKMAWPPLVHRIVRTVRLRSLFQRGQ
                     HIVVAISGGPDSVALLSILHLLRSSWKLSLSAVHCNYGLRGAESDEDQKFVEAFC"
     gene            complement(275..1231)
                     /locus_tag="VN647_04770"
     CDS             complement(275..1231)
                     /locus_tag="VN647_04770"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWG96379.1"
                     /translation="MKVTRGYSDEGVRPYPVGTIGNFDGHHLGHHALLKRVVERARRV
                     QGTAVVLTFDPHPVKILAPHVDLRFLTSWEEKRARFEQAGIDEVISVEFTQVFASLSP
                     AMFAEQVLSKGLGLKEIFVGQHFAFGHKRVGQIDDLTALGERFGFIVHPTPPVMINGG
                     VVSSTSIRQLIVAGHVDQAAVLLGRHYALSGVVSPGEGRGRTLGCPTANLRLPPDRVA
                     PADGIYASVVTIDQERHDSVAYIGTRPTFATFDGAGRGLEVSILDGIHELYGRTLTVE
                     FIDRIRGDAQFDSAEALRRQITADVDSARGILRRYHETIGGR"
     gene            complement(1238..2209)
                     /gene="hemB"
                     /locus_tag="VN647_04775"
     CDS             complement(1238..2209)
                     /gene="hemB"
                     /locus_tag="VN647_04775"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWG96380.1"
                     /translation="MGFPIQRLRRLRQHESLRRMVRETALSPSDFIYPLFVIEGQDRR
                     EKIASMPGQFRLSVDLLVKEAGEIQALGIPAIILFGIPAHKDERGSSGWDPNGIVQRA
                     IRAVKQQVPGLLVVTDVCIDEYTSHGHCGIVKDGKVLNDETLECLTVMARTHAEAGAD
                     MVAPSDMMDGRVAAIRRELDRSGFSDLPILAYAAKFSSCFYAPFRDAAFSSPQFGDRQ
                     SYQMDPANAREALREIDLDIDEGADIVMVKPALPYLDIIAMARARTLLPLAAYQVSGE
                     YSMIKAAARAGWLDESRAMMESLLAIKRAGADLILSYFAKDAVKLLH"
     gene            complement(2217..2609)
                     /locus_tag="VN647_04780"
     CDS             complement(2217..2609)
                     /locus_tag="VN647_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG96381.1"
                     /translation="MVPVKSFMVPREKFVTVPRDTDAQTAARIMRDRGIGSLFVTNDR
                     EIIGIITDTDMVRRVVAAGADAIKTTVEQIMSAPIMTIEESKTLLDANDLMAQSHLRH
                     LGVTRDGKLVGVISVRDLVVFLTNLPRK"
     gene            complement(2659..4227)
                     /gene="cobA"
                     /locus_tag="VN647_04785"
     CDS             complement(2659..4227)
                     /gene="cobA"
                     /locus_tag="VN647_04785"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWG96382.1"
                     /translation="MNKVRRHKGKVYLVGAGPGDPGLLTLRGKECLEQADVVLYDYLA
                     NPTLLAYAQDRVERIYVGRRGKGKYPEQEAINRLLIERASAGNVVVRLKGGDPFVFGR
                     GGEEAEVLATAGIEFEVVPGVTAAVAAPAYAGIPVTHRTLASTLTIVTGHEDPEKPST
                     ALDWSRLATSQGTVVFLMGMKNLSTIAATLMAEGQAGSTPVAIIRWGTRVSQQTVVGT
                     LADIADKADAAQMEPPTVIVVGEVVRLRPKLNWFERRPLFGKRVLMTRAKEQAGELAV
                     RLAAYGAEPVEAPTIKIVPPLDWEPVDHAISEIGTYDWIIFTSVNGVNRFMTRLLACG
                     RDSRCLAGRQLCCIGPRTAQELEKFGVRADLVPTEYQAEGVLTALNRQHLKNPRILIP
                     RAEVARELLPDELRAAGADVAVVSVYRTLTPDQDSGGWRQELMDHRIHVVTFTSSSTV
                     RNFVAMLGGVEAVKPLLQSVTIACIGPITATTAEEYGLTVSLMPSENTIPALADAIAH
                     HYGSREQVTTGVLQ"
     gene            complement(4224..>4640)
                     /locus_tag="VN647_04790"
     CDS             complement(4224..>4640)
                     /locus_tag="VN647_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWG96383.1"
                     /translation="GLHRLGWSQTITEYLSPILSLPAVGQGALGIEGRTNDAFVRSVL
                     SRLDHPATHTTVTAERALLRRLEGGCQVPIAAHATLSGERLVLDGLVAGIDGKTVIRD
                     RIEGTHQEAHTLGVQLAERLLIRGGDKILRVIYGSA"
BASE COUNT         1056 a         1413 c         1186 g          985 t
ORIGIN      
        1 gcaaaaggct tccacaaatt tttggtcttc gtcggactct gcccctcgca gtccatagtt
       61 gcaatgaacg gctgataggc tcagcttcca actcgaccgg aggagatgca ggatcgataa
      121 cagtgcgacg gaatccgggc cgcccgagat ggcgaccact atgtgctgtc ctcgttgaaa
      181 gaggcttctg agtcgtaccg ttcgaacgat acgatgcacc agcgggggcc aggccatttt
      241 atcgataggt ctgtttaggg tcggttgcaa agactcatcg ccctccgatt gtttcatgat
      301 atcgacggag aatgcctctc gcagaatcca cgtcggccgt gatttgccgc cttagagctt
      361 ctgcgctatc aaactgcgcg tcgcctcgta tacgatcgat aaattcaacc gtgagcgttc
      421 gtccatacaa ctcgtgtatc ccatccagga tggatacttc caatcctcgt cccgccccat
      481 cgaaggtcgc aaaggtcggt ctagtaccga tataggcgac cgaatcatgt cgctcctgat
      541 cgatggttac gaccgaggca taaattccat cggcaggagc cacgcggtct ggaggcaatc
      601 gaagatttgc ggtaggacat cctaatgttc gccccctccc ctcgccagga gatacgaccc
      661 cactcagagc atagtgtcgg ccgagcaaga cagccgcttg atcgacatgc cctgcgacaa
      721 tgagttgcct gatactcgtc gagctgacta cccctccatt gatcatcacc ggaggagtcg
      781 gatgaacgat gaaaccgaac cgttcgccga gcgcggtcaa atcgtcgatc tgtccgactc
      841 gcttgtgccc aaaggcaaaa tgttgcccca caaaaatctc tttgagtccg agccctttag
      901 acaaaacctg ctcagcgaac atcgccgggg aaagtgacgc aaatacttga gtgaactcca
      961 cggaaatcac ttcatcgatt ccggcttgtt cgaaacgggc acgtttctcc tcccagctcg
     1021 tcaggaaccg tagatcaacg tgaggggcaa gaattttcac cgggtgaggg tcgaatgtca
     1081 ggacgacggc cgtgccctgg acgcggcgtg ccctctccac cacccgtttc aaaagcgcat
     1141 gatgaccgag atgatggcca tcgaaattcc cgatggtgcc caccggatat ggccgtacgc
     1201 cctcgtccga atatccgcgg gtcactttca tcgtcgatca atgaagcagc ttaacggcat
     1261 ctttcgcgaa atacgacagg atcagatcgg ctcccgcccg cttgatcgcc aacaacgatt
     1321 ccatcatggc acgtgattca tcgagccacc ctgctctcgc tgccgcctta atcatactgt
     1381 actcgccgct cacctgatac gccgccagcg ggagaagcgt ccgagcacgg gccatcgcga
     1441 taatgtccaa ataaggcagc gccggcttga ccatcacaat atcggcacct tcgtcgatat
     1501 cgagatcgat ctcgcgcagc gcttcacgcg cattagccgg atccatctga taggactgtc
     1561 ggtcaccgaa ttgaggactg gaaaaggccg cgtcgcgaaa aggtgcatag aaacaggagg
     1621 agaatttggc ggcatacgcc aggatcggca gatcggagaa cccggagcga tccagttcac
     1681 gtctgattgc agcgacacga ccatccatca tatccgacgg tgcgaccata tcggctccgg
     1741 cctcggcatg agtacgtgcc attaccgtga gacattcgag cgtttcatca ttgagaactt
     1801 ttccatcctt gacgatcccg caatggccgt ggctggtata ctcgtcgata cagacatccg
     1861 tgaccacaag taatcccggc acttgttgtt tgacagctct gatcgcccgc tgcacaatgc
     1921 cattgggatc ccaccccgag ctaccacgct cgtctttgtg cgccggtatc ccaaagagaa
     1981 tgatggctgg aatacccaag gcctgaatct ctcccgcctc tttgaccagc agatcgacgg
     2041 agagccgaaa ttggcccggc atcgaagcaa ttttttcacg gcgatcctgt ccctcgatca
     2101 caaacagcgg atagataaaa tccgacgggg acagcgccgt ttcccgtacc atccgacgca
     2161 acgattcatg ttgtctcagt cgtcggagcc gctggatcgg aaaccccata gcccgcctac
     2221 tttcgtggaa ggttcgtcaa gaacacaacg aggtcgcgaa cggaaataac cccgaccagc
     2281 ttcccatcgc gcgtgacacc gagatggcgg agatgggatt gcgccatcaa atcgttggca
     2341 tcgagcaacg tcttgctctc ctcaatggtc atgatcggcg ccgacatgat ttgctcgacc
     2401 gtcgtcttga ttgcatccgc cccggccgca actactcgac gcaccatgtc cgtatccgtg
     2461 atgatgccga taatctcgcg atcgttggtc acgaacaaac tgccgatccc acgatcgcgc
     2521 atgatgcggg cggccgtctg tgcatctgta tcacgaggca ctgtcacgaa cttctctcgg
     2581 ggaaccataa acgactttac aggaaccatc tcaccctccc tccttacata atatataagt
     2641 acatctcacg tgctgtcgtt actgcagaac tcctgtggtc acctgctcac ggcttccata
     2701 atgatgggcg atcgcatcgg ccaatgcggg aatcgtattc tcactcggca tgagcgagac
     2761 cgtcaaacca tattcctctg ccgtcgtggc cgtaatgggt ccgatgcaag cgatcgtgac
     2821 tgattgtaag agcggcttca ccgcttctac accgccgagc atcgcgacaa agttgcggac
     2881 cgtggaagag ctggtgaacg tgacgacatg aatccgatga tccatgagtt cctgccgcca
     2941 ccccccggaa tcttggtctg gtgtgagtgt gcggtacacg gagacgacgg cgacatccgc
     3001 cccggcggca cgaagttcgt ccggcaagag ctctctggcc acttccgccc gaggaatcag
     3061 gatacggggg ttttttaaat gttgccgatt cagtgcggtt aacactcctt ccgcctgata
     3121 ttctgtcgga accagatccg ctctgacacc gaacttttcc aactcttgag ccgtgcgcgg
     3181 cccgatacaa caaagctgtc gtccagccaa acagcgtgag tcacgtccac aagccaatag
     3241 cctggtcatg aaacggttca ccccattgac actggtaaaa ataatccagt cgtacgtccc
     3301 gatctcggag atcgcgtgat cgacaggctc ccagtcgagc ggaggaacga tcttgatcgt
     3361 cggagcttcg accggttcag ctccataggc ggctaatcgg acagctaact caccggcttg
     3421 ttctttcgct cgcgtcataa gcacccgttt tccaaagagc ggacgccgct cgaaccagtt
     3481 gagtttcggt ctcaagcgaa cgacctctcc cacgacaatg acggtcggcg gctccatctg
     3541 tgcggcatcg gctttatccg caatatcagc caaggtgccg acgacggtct gctgtgaaac
     3601 cctcgttccc catcgaatga tggcgacggg agtggacccc gcttgccctt cggccattaa
     3661 ggtcgcagcg atcgtcgaaa gatttttcat gcccatgaga aaaaccacgg taccttggct
     3721 cgtcgccagc cttgaccagt ccaaagccgt ggaaggcttc tctggatctt catgtccggt
     3781 gacaatcgtc agcgtcgacg ccaacgttcg atgcgtcacc ggaataccgg catatgcagg
     3841 ggcggcaacc gcagccgtca cccctggaac aacttcgaat tcaatcccgg ccgtggcgag
     3901 cacttccgct tcttcgcctc ctcgcccaaa gacaaacgga tcgccaccct tcaaccgtac
     3961 gactacattg cctgcgcttg cccgttcaat cagcagacgg ttgatggcct cctgctcagg
     4021 gtattttcct ttacctctcc gtccgacata gattcgctcg actcgatcct gagcataggc
     4081 aaggagggta ggattagcca gataatcgta gagcaccacg tcggcctgct caagacactc
     4141 ctttcctcga agagtcaaga gcccaggatc tccaggacca gctccgacca aatagacctt
     4201 ccctttatgt cgtcgtacct tgttcatgct gatccgtaaa tcacgcgaag aattttgtcc
     4261 cctccccgaa tcagtagccg ttcagccaac tgaacaccca gggtgtgagc ttcctgatgt
     4321 gtcccctcaa tccggtcgcg aatgacggtc ttcccatcga taccggcgac aagaccatcc
     4381 aagaccagcc gctcaccgga cagggttgcg tgggctgcga tgggcacctg acacccaccc
     4441 tctaggcgac gcaagagcgc ccgttctgcc gtcactgtcg tatgggtcgc aggatgatca
     4501 agtcgggata agacggaacg cacgaatgca tcgttggtcc gaccttcgat accgagggct
     4561 ccctgtccga ccgcaggcag actgaggata ggggaaagat attccgtgat ggtctgagac
     4621 caacccaacc ggtgcagacc
//
LOCUS       DATNDD010000198         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2289579, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000198 DATNDD010000000
VERSION     DATNDD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289579"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="pstC"
                     /locus_tag="VN647_04795"
     CDS             <1..999
                     /gene="pstC"
                     /locus_tag="VN647_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWG96384.1"
                     /translation="IEGFRKDTGRGWLFMDKSLAAVREVIRISEPVIVEATARRRTQH
                     RREQVIESGLFFAALMSVAATVAILTVLLWESLGFFKAVSLTDFLTDTLWTPLFDDAH
                     YGILPLLAGTVVTSLVGLLVAIPVGTVSAIYLSEFASSRLRELIKPVLELLGAVPTVV
                     YGYFALLVVTPALQKLWPDLPGFNMLGPGIVIGIMILPLVISLSEDAMRAVPMVLREG
                     SYATGATRLQTAWRVVVPAATSGLVAAYVLGVSRAVGETMVVAIAAGQNPNLTWNPME
                     PAATITAYIVQVALGDLPHGSIGYQSIFAAGLTLAVMTFTFNMLGHVMRKRFREVY"
     gene            999..1889
                     /gene="pstA"
                     /locus_tag="VN647_04800"
     CDS             999..1889
                     /gene="pstA"
                     /locus_tag="VN647_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWG96385.1"
                     /translation="MLRRRKLAETLFKAVGICSLALGIGTLVLLFGALIVEGAGRIDW
                     QFLTSFPSRRATQAGILPALVGSSLIMLVTAVVGIPLGVAAAVYLEEYARRTWLTELI
                     EVTVTNLASVPSIIFGLLALGFFVYILGLGASILTAGLTLALLILPVVIVTTREAIRA
                     IPVQIREAAFALGATKWQTVSDHVLPYSAAGILTGIILALSRAIGETAPIVTIGALTF
                     IAFLPPAPFATEPPFISFEWLMSPFTVMPIQMFAWVSRPGEDFASNAAAAGLLLVCMT
                     LGMNGLAIYVRYRMRKRIAW"
     gene            1900..2706
                     /gene="pstB"
                     /locus_tag="VN647_04805"
     CDS             1900..2706
                     /gene="pstB"
                     /locus_tag="VN647_04805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWG96386.1"
                     /translation="MELPRIESPEDRVLPKPLKAEARSLSFSYGSRLALKNLSIPIAE
                     YKITALIGPSGCGKSTFLRCFNRMHDLYPGTCYEGEILLYPDGRNILAREVDPIEVRM
                     RLAMVFQKPNPFPKSVYENVAYGLKVRGVMDRRILDEKIEQALRSAALWEEVKDRLSA
                     QATSLSGGQQQRLCMARALATDPELLLLDEPTSALDPIATANIEELLLDLKRRVTILI
                     VTHNMQQAARVSDWTAFMYLGELVEFGLTKQLFTNPSRKQTEDYITGRFG"
     gene            2711..>3193
                     /gene="phoU"
                     /locus_tag="VN647_04810"
     CDS             2711..>3193
                     /gene="phoU"
                     /locus_tag="VN647_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWG96387.1"
                     /translation="MIQRHFDEELAELKTKLARMAGLAEDQIDKALAALITRDSALAC
                     RVIERDHKVNALDVEIDEACIELLALHQPAARDLRLVTTAMKISTELERISDLSENIS
                     ERAIELNEEPQLKPYIDIPRMGTLARVMVKESIDAFVKEDSKLARKVLVDDDLVDDLM
                     E"
BASE COUNT          643 a          849 c          928 g          773 t
ORIGIN      
        1 atcgagggat tcaggaaaga tacaggaaga ggttggctgt tcatggataa gtctctagcg
       61 gctgtgcggg aagtgattcg tatcagcgaa ccggtcatag tagaagcaac cgctcggcgt
      121 cggactcaac accggcgaga acaagtaatc gaaagcgggc ttttctttgc cgcgctgatg
      181 tcggtagctg ccacggttgc gattctcact gtgcttttgt gggaatcgct cgggttcttc
      241 aaggccgtgt cgctcacgga ctttttgacc gatacattat ggacaccgct ctttgatgat
      301 gcccactatg gaatcctgcc cctgctcgca gggacggtag tcaccagcct ggtcggtctg
      361 ctcgtggcga ttcccgtggg gacggtgtcg gcgatctatc tctcggaatt tgcctcgtct
      421 cgcttgcgag aactcatcaa gccggtgttg gaactgctcg gagcggtacc gacggtcgtg
      481 tatgggtatt ttgcgcttct tgtggtcacg cccgctctgc agaagctttg gccggacctc
      541 cccggcttca atatgctggg tcctggaatc gtcatcggca tcatgatatt gcctctcgtt
      601 atttctctga gtgaggatgc gatgcgggct gtgcccatgg tgttgcggga aggatcctat
      661 gcgacgggag cgacacgtct tcagacagct tggcgtgtgg tagtcccggc agccacttcc
      721 ggccttgtgg cagcctatgt gctcggagtc tcgcgtgccg tcggtgaaac gatggtggta
      781 gcgatcgcag ccgggcaaaa tccaaatctc acgtggaatc ccatggagcc tgcggccacc
      841 attacggctt atatcgtgca agtggccctg ggtgatctcc ctcatggcag catcggctac
      901 cagagcattt ttgctgccgg gcttaccctt gcggtgatga cgtttacctt caatatgctg
      961 ggccatgtca tgcgaaaacg gttccgggag gtttattgat gttgagacgc cgtaaacttg
     1021 cggaaacctt gttcaaggcc gtggggatct gttccctcgc cctcgggatc ggaacgctgg
     1081 tgttgttgtt cggcgcgctc atcgttgaag gggccggccg aatcgactgg caatttctca
     1141 catcctttcc gtctcgtcgc gccacacagg ctggtattct acctgcgttg gtcggctcga
     1201 gtttgatcat gctggtcacg gctgtggtgg gcatcccctt gggggtcgct gcggcagttt
     1261 acttggagga atatgcccgc cgaacgtggc tgacggagtt gatcgaggtg acggttacca
     1321 atctggccag cgtgccgtcg attatatttg gcctactggc attagggttt ttcgtctata
     1381 ttctaggtct tggggcgagc attctgacgg ctggcctcac cttggctctg ctcattcttc
     1441 ccgtcgtgat cgtgacgaca cgagaggcca ttcgcgcgat tccggtgcag atccgcgaag
     1501 cggcctttgc tttgggtgcg accaaatggc agacggtctc cgatcacgtg ttgccctatt
     1561 ccgcggctgg gatcctgacc gggatcatct tggccttgag ccgggcgatc ggagagacgg
     1621 cacccatcgt gacgatcggc gccctcacct ttatcgcctt tctacccccg gccccattcg
     1681 cgacggaacc tccgtttatt tcgttcgagt ggttgatgtc tcccttcacg gtcatgccca
     1741 ttcagatgtt cgcatgggtt tcaaggccgg gcgaagactt cgcgagcaat gcggcggctg
     1801 caggattgtt gctggtctgc atgaccctcg gaatgaacgg cctggccatt tacgtacgtt
     1861 atcgcatgcg caagcgtatc gcttggtaat atggacctca tggagttacc acgtatcgaa
     1921 tcacccgagg atcgggtttt gcctaagcct ctcaaggctg aggcccgcag cttgagtttt
     1981 tcctatggga gtcgattggc attgaagaat ttgtccatcc ccatcgcaga atacaaaatc
     2041 acggcattga ttggaccgtc tggttgcgga aaatccacct tcctccgttg ttttaaccgg
     2101 atgcacgatc tctatcctgg gacctgttac gagggagaaa tcttgctcta cccggatggc
     2161 cgcaacatcc tggctcgcga ggtggaccca attgaggtac gcatgcggtt ggccatggta
     2221 tttcaaaaac ccaatccttt ccccaaatcc gtctatgaaa atgttgctta tgggttaaag
     2281 gttcgcggag ttatggatcg gcgaatcttg gatgagaaga tcgaacaggc gttgcgaagt
     2341 gcggcattat gggaagaggt caaggatcga ttgtcggcac aagcaacatc cctatccggc
     2401 ggccaacagc aacgtctctg tatggcccgc gccctggcga ccgacccgga actgctgctc
     2461 ctggatgagc cgacgtcggc gctcgacccg attgccacgg ccaacatcga agaactgttg
     2521 ctcgatctca agcgacgcgt gaccattctg atcgtgacgc ataacatgca gcaagcagcg
     2581 cgggtgtcgg attggacggc tttcatgtat cttggtgaac tcgtcgagtt cggcctcacg
     2641 aagcagctgt tcacgaatcc atcaaggaaa cagaccgaag actatattac cggacgattc
     2701 ggttaggctt atgattcagc gacacttcga cgaagaactt gccgaattaa aaaccaagct
     2761 cgcacgaatg gcaggtctag cagaggacca aatcgataag gcattggctg ccttgattac
     2821 tcgcgattcg gccctcgcct gtcgcgtgat cgaacgagac cacaaggtga atgcactcga
     2881 tgtggagatc gacgaggcct gtatcgagtt gttggcgctc caccaaccgg ccgcgcgcga
     2941 tttacggctc gtcacgacgg ccatgaaaat ctccaccgag cttgagcgga tcagcgatct
     3001 ctcggaaaat atctcggagc gcgcgatcga gctgaatgaa gaaccgcagc tcaagccgta
     3061 catcgatatt ccgaggatgg gaaccctcgc aagagtgatg gtgaaggaaa gcatcgatgc
     3121 cttcgtcaaa gaggattcca agctcgctcg gaaagtgctc gtggatgacg atctggtcga
     3181 tgatctgatg gaa
//
LOCUS       DATNDD010000199         3606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3252416, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000199 DATNDD010000000
VERSION     DATNDD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3252416"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..506
                     /gene="thiS"
                     /locus_tag="VN647_04815"
     CDS             306..506
                     /gene="thiS"
                     /locus_tag="VN647_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWG96388.1"
                     /translation="MQVKINGKPEEIQSGTVLDLLKTKNIEPQMVAVEVNDKVLDRDL
                     LATTRLNEGDHVEFLFYMGGGR"
     gene            503..1432
                     /gene="cysK"
                     /locus_tag="VN647_04820"
     CDS             503..1432
                     /gene="cysK"
                     /locus_tag="VN647_04820"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWG96389.1"
                     /translation="MSTALHNDITELIGKTPLVRLNRLSKSGSATIYGKVEFFNPGGS
                     VKDRICLSMINEAERQGKLKPGGTIVEPTSGNTGIGLALVAAVRGYKLLLVMPESMSM
                     ERASLLSSYGAQLVLTPAWEGMKGSIKEAESILAQNPSYFMPDQFSNAANPAMHRMTT
                     APEIWDALEGKIDAFVAAVGTGGTITGCGEVFKEQNPQIKVIAVEPATSPVLSGGDPG
                     PHKIQGIGAGFIPKVLNRKILDRVITVTDDEAYQTAKQLSKKEGLLVGISAGANVFAA
                     QKIADELGPDKNVVTILCDTGERYISIEKYFNI"
     gene            1627..2445
                     /gene="moeB"
                     /locus_tag="VN647_04825"
     CDS             1627..2445
                     /gene="moeB"
                     /locus_tag="VN647_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWG96390.1"
                     /translation="MDFTEEQINRYSRHILLPEVGGKGQRKIAKSRILLVGAGGLGSP
                     AALYLAAAGVGTIGLIDSDVVDLTNLQRQILHHTSDVGRPKVLSGKEKIQALNPDVSV
                     SMYEERLTAGNALKIFSDYDVVIDGVDNFTAKFLINDACFFAGKPLVHGGILRFDGRV
                     TTIVPGKSACYRCVFKAPPPPGFVASCQEAGVIGVLAGIIGTIQATEALKLVLGIGRP
                     LTDRLLDFDARKTQFREIKVRRNPNCALCGEHPRITELFDDGDPYAGCAMHPSA"
     gene            2468..>3606
                     /gene="thrC"
                     /locus_tag="VN647_04830"
     CDS             2468..>3606
                     /gene="thrC"
                     /locus_tag="VN647_04830"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWG96391.1"
                     /translation="MSKMKALVCRECGKEYPTKAIHVCEMCFGPLEVKYNYDEIRKTI
                     SRKKIEDGPRSMWRYLDLLPVKGTNFVGPHAGFTPLVRAKNLGAYLGLDELYIKNDTV
                     NHPTLSFKDRVVAVALTRARELGFETVACASTGNLANSVAAHAASASLHCYVFIPGDL
                     EAAKVLGNLIYKPHVVEIEGNYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWKTPDQVVIPMASGSLLTKIWKGLHEMKALGLIDTVHTKINGAQAEGCSPI
                     STAFKAGRDFFKPVKPQTIAKSLAIGNPADGYYALKATAESHGSMDMVTDEEVVEGIQ
                     LLAQTEGIFAETAGGVTIGVLKKLVKQGVIKKDEVTVAYVTGNGL"
BASE COUNT          867 a          977 c         1015 g          747 t
ORIGIN      
        1 ctgtccagcg ccggttccgt atcatggaac aactcgttct ctgactcagt caacggtatc
       61 gattcagcag cgcgaaagag aaagttcttt ttccacatgc aagcggcata gtggcggcga
      121 gccggatcaa agtcaagcaa cccctcagga cactctcctc ttctcaacag acgctgttga
      181 gcatgaatcc tattgtgcgt tggtgtctct cactactggt cattgaacaa cacagccttg
      241 acaagcagag catgcctttg ctagcttcaa aattctttaa tctctaagcc aacttgggag
      301 aatctgtgca ggtcaagatc aatggaaagc ctgaggaaat ccagagcgga accgtcctcg
      361 atttattgaa gacaaagaac atcgagccgc agatggtcgc cgtcgaggtc aacgacaagg
      421 tattggatcg tgatcttctt gccacaaccc gcctcaatga aggagaccat gtagaatttc
      481 tcttttatat gggagggggc cggtgagtac agcgctgcac aacgatatca ccgagctgat
      541 cggcaagact ccgcttgtgc ggttgaaccg cctttcaaag tccggttcgg cgacgattta
      601 cggcaaggtc gagtttttca atcccggtgg cagcgtcaag gatcggatct gcctcagcat
      661 gatcaacgag gcggaacgtc aagggaagct caagccgggc ggaaccatcg ttgagccgac
      721 gagtggaaat acgggaatcg gattggcctt ggtcgcggcg gttcgcgggt acaaactgct
      781 ccttgtgatg ccggaaagca tgagcatgga acgggccagc ttgctgtcgt cgtatggcgc
      841 gcagctggtg ttgacaccgg catgggaggg catgaagggg tctattaagg aggcggaaag
      901 tatcttggcg caaaatccat cgtactttat gccggatcaa ttctcgaatg ccgccaaccc
      961 agccatgcac cggatgacga cggcaccgga aatttgggat gctctcgaag gcaagatcga
     1021 tgcgtttgtg gcggctgttg gaaccggcgg aaccatcacg ggatgcggtg aagtcttcaa
     1081 ggaacagaac ccacagatca aagtgatcgc tgtcgaaccg gcgacgtcac cggtcttgtc
     1141 cggcggcgac ccaggaccgc ataagattca agggatcggt gcgggcttta ttcccaaagt
     1201 cctcaaccga aaaattctcg accgcgtgat caccgtgacg gatgacgagg cctatcagac
     1261 cgcgaaacag ctctcgaaga aggaaggcct gctggtcggc atttctgccg gtgccaatgt
     1321 gttcgccgcc caaaagatcg ccgacgaact gggccccgac aagaatgtcg ttacgatcct
     1381 gtgcgacaca ggtgagcgct acatcagtat tgagaaatac ttcaatattt gacgatcaac
     1441 cagttattag gagggatgct caaaatggtc gccggcgagg ccgcagcgag aagaatccgg
     1501 aggcgtattc gtttacaccc gcccgccccg agctgccaag acagctcttc gcctcgatgg
     1561 ggtacgttga ggagttcttc gagccgagaa caacgctgga gaccattttg agcatccctg
     1621 accgacatgg attttactga agaacagatc aaccgttaca gccggcatat cctgctgcct
     1681 gaagtcggcg gcaaggggca gaggaaaatc gccaaatccc ggattcttct cgtcggtgcc
     1741 ggcggcctcg gctcccctgc cgcattgtat ttggctgcgg caggagtcgg caccattgga
     1801 ttgatcgaca gcgacgtggt ggacctgacc aatctccaac gccaaattct tcaccatact
     1861 tccgacgtgg gccgtcccaa agtcctctcg ggcaaagaaa agatccaagc gttgaacccc
     1921 gatgtctccg tgtcgatgta cgaagaacgg ctcacggccg gaaacgcgct taagatcttc
     1981 agtgactacg atgtcgtcat cgatggtgtc gataatttca cggcgaagtt tctgatcaac
     2041 gacgcctgtt tcttcgcggg gaaaccgctg gtccatggcg gcatccttcg attcgacgga
     2101 cgtgtcacga ccattgtccc cggcaaatcg gcctgctatc gctgcgtatt caaggcgcct
     2161 cctccgcccg gctttgtcgc atcctgccag gaagccggag tcatcggagt cttggccggc
     2221 attatcggaa cgattcaagc gacagaagca ttgaagctcg tattaggcat cggacggccg
     2281 ctgacggacc gcctgctcga cttcgatgcg cgcaaaaccc aattccgaga aattaaagtc
     2341 cgccggaacc cgaattgtgc gctctgcggg gaacatccga ggattaccga gctgttcgac
     2401 gacggggacc cctatgccgg atgcgccatg catccctctg catagggttt gataaggtgg
     2461 tgcgacgatg agcaaaatga aagcgctggt ttgccgcgag tgcggcaagg aatacccgac
     2521 aaaggcgatc catgtctgcg aaatgtgctt cggtcctctt gaggtgaagt acaactacga
     2581 cgagatcagg aagactattt cgcgtaagaa gatcgaggat ggtcctcgca gcatgtggcg
     2641 ctacctcgac ttgctgccgg tcaaagggac gaacttcgtg ggaccgcacg cgggatttac
     2701 cccgctcgta cgcgcaaaaa atctcggcgc ctatcttgga ctcgacgagc tttatattaa
     2761 gaacgacacg gtgaaccatc cgaccctgtc gtttaaagat cgcgtcgtcg ccgtggccct
     2821 cacgcgcgcg cgcgagctcg gtttcgaaac agtagcttgc gcatcgaccg gcaacttggc
     2881 gaactcagtc gccgcccatg ccgcatccgc cagtcttcac tgttatgtat tcatccccgg
     2941 cgatcttgag gccgcgaagg tactcggcaa tctgatctat aagccgcacg tcgtcgagat
     3001 agaaggcaac tatgacgatg tcaatcggtt gtgcagtgag atcgccggcg aacatggatg
     3061 ggcgttcgtg aatatcaaca ttcgtccgta ctatgccgaa ggctccaaaa ccctcgcctt
     3121 tgaaaccgtg gaacaactgg gctggaaaac gccggatcaa gtcgtcattc cgatggcgtc
     3181 aggttctctt ttgaccaaga tttggaaggg gctgcatgag atgaaggcct tgggcctcat
     3241 cgacaccgtt cacacaaaga tcaacggtgc ccaagcggaa ggctgctcac cgatctcaac
     3301 cgcattcaag gcgggacgcg acttcttcaa acctgtgaaa ccacagacga tcgccaagtc
     3361 ccttgccatc ggcaaccctg ccgatgggta ctatgcgctc aaggcgaccg ccgagagcca
     3421 tggatccatg gacatggtga cggatgaaga agtggttgaa ggcatccaac tgctggccca
     3481 aaccgaaggt atcttcgcgg aaacagccgg aggcgtcacg atcggtgtgc tcaagaagct
     3541 cgtcaagcaa ggcgtgatca aaaaagatga ggtgacggtg gcctatgtca ccggcaatgg
     3601 attgaa
//
LOCUS       DATNDD010000200         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2611251, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000200 DATNDD010000000
VERSION     DATNDD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611251"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VN647_04835"
     CDS             <1..196
                     /locus_tag="VN647_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96392.1"
                     /translation="EDIAVASTEPMSWPEIPMTYLIAAALLLATVFLGGLSMAAQSRE
                     AKALAQLRRDADRWAFRMAS"
     gene            272..1003
                     /locus_tag="VN647_04840"
     CDS             272..1003
                     /locus_tag="VN647_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG96393.1"
                     /translation="MKREISTSNRNQRNTAHRIDTGAGPRSIRLLIVEAQRLFRQSLR
                     VLLERERNIAAVVEATDGREAYRLAMEHKPDIVLLDVDMPDLDVESVTKLVRKHLPNT
                     RVLLLARYDEDSRIVAAMQAGAFGYILKDTDCTDFLRIIKATMSGEHILSPVIPERIV
                     RTVPGAVRQTHEDNNLLLSSLTDREREILACAAAGRGNKEIADQLCVSIDTVKTHLHH
                     IYQKLSVDGRVEAILTYLKGSVISF"
     gene            1686..2051
                     /locus_tag="VN647_04845"
     CDS             1686..2051
                     /locus_tag="VN647_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96394.1"
                     /translation="MNAVKNNRPTSASHWVRIPDGTKVRHRSEAYEGIIDGLTEIVSG
                     SDRNPDGKTQYRVNVGDSTRLLVSEDHLNILLDNKNLVLLARESELYRRNVTDRLRAV
                     FAEDRFVRIGSKEPTSPGK"
     gene            2115..2384
                     /locus_tag="VN647_04850"
     CDS             2115..2384
                     /locus_tag="VN647_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96395.1"
                     /translation="MNTSWGTKASHRRKRAIRRTKVRWDLLGLQDPEQTPLTTSIDDI
                     RRQIISAASQGLSPDTISRSHAKAGRPKPGSKASVATRKPGAASQ"
     gene            complement(2605..>3019)
                     /locus_tag="VN647_04855"
     CDS             complement(2605..>3019)
                     /locus_tag="VN647_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96396.1"
                     /translation="KELIIRGGVNIAPLEIDEVLRSHPLVRFAMTVPFEHRYYGEEIA
                     AYVVPRDGVSPPTEAEVLAHCRQRLPFSKCPKVIRFGQNVPYTSTGKPKRLELKISLA
                     PTLAAYRDYQFKDAEVVQIGEANQRRIDASGGADQ"
BASE COUNT          714 a          798 c          837 g          670 t
ORIGIN      
        1 agaagatatt gccgtggctt ctactgaacc tatgtcatgg ccggagatcc caatgaccta
       61 cttgattgcc gctgctcttc tgttggccac cgtctttttg ggaggccttt ccatggcggc
      121 gcaaagcaga gaagcgaagg cgcttgctca gttgagacgg gatgccgacc gctgggcgtt
      181 tcgcatggcg agctaactga agtatgaacg gttacaccgg tgctcgacag aatcagtagg
      241 cgcgtgtcgg gcaccggtga gtcggattcg tatgaagagg gaaatctcta cgtcaaatcg
      301 taaccagagg aatacagccc atcgcatcga tactggagca ggaccccgat ccatcagact
      361 gctcattgtc gaagcccaac ggctgttcag gcaaagtctg cgggtgctct tggaacgaga
      421 acgcaatatc gctgccgtgg tggaagcaac ggatgggcgc gaggcctacc gactagcgat
      481 ggaacacaag cctgatatcg tcctcctgga cgtggatatg ccggatctcg atgtggaatc
      541 ggtcacgaaa ctcgtccgca aacacctccc caacacgcga gtgctgctgc tggctcggta
      601 cgatgaggac agcagaattg tcgcagccat gcaggctgga gcgttcggtt acatcctgaa
      661 ggataccgat tgtacggact tcctgcgcat catcaaagcc accatgagcg gagaacacat
      721 cttgtcaccg gtcatccccg aacgcatcgt ccgcactgtt cccggtgcgg tgcggcaaac
      781 ccatgaggac aacaaccttc tgctttccag tctgactgac cgtgaacgag agatattggc
      841 ttgcgcggct gccggtcgag gcaacaagga aattgccgac cagttgtgcg tttcgatcga
      901 taccgtgaaa acacatctgc atcatattta tcaaaagctg tccgtcgacg gacgtgtcga
      961 agctattctc acctatctca aaggctcagt gatctctttt tagccggcac tcactcaatc
     1021 ggtgtaggtc gcaattcact cttgtaggcg atagcaccga agcggtctac ctcttaacat
     1081 gaaggcaaat gttgagattg gtccagacac ctttacttct aaagagtgtt tgagtggttg
     1141 ttcaaccaag gaggtgtacg ccatgcatgt tgacattaag gatgagttaa agcacgagcc
     1201 ggcatgggcg ttcttgtatg cagcgttctt tatcgggatc gtggtctttg cacttctgtc
     1261 cggcggtttc atgggatgat cgatctgtga acgattgttg cagctagagt atgatcaagt
     1321 gagcgcctac cgccgaggcg gaaatgcccg ccggagccgt ccataaacgg ctccggtatt
     1381 tcctttccaa tgatgactcc tcttcttacg gtagctcttt cgtgttcgca atttatccta
     1441 tcatgtgata gactacacta tgcagatttg ttcctcgcgc tcttcccctc cgttccgttc
     1501 ctgcgcgatt gagttgatca gccccttgcg gtgacttcgt cgggcaaacg ctctgcttgt
     1561 cactcgtcgg aggaatccat ggagctgaca tctatgctcg tcggttttcg tcagtctctt
     1621 tttggtttag caggccgcag aaggaggaca atagagatgg gtggtcatgt agggagtgaa
     1681 cgtccatgaa tgccgtcaag aataatcgac cgacgtctgc gagccattgg gttcgcatcc
     1741 cggacgggac caaagtgcgc catcgctctg aagcctatga agggatcatt gacgggttga
     1801 ccgaaatcgt gtctggttcg gatcgcaatc cggacgggaa aacacaatat cgtgtgaacg
     1861 tgggcgacag tacgcggtta ctcgtctcag aggatcacct taatattctg ctcgacaaca
     1921 aaaatttggt tctgctcgct cgcgaatccg aactgtatcg tcgaaacgtc accgaccgcc
     1981 tacgggcggt atttgcggaa gatcgattcg taaggattgg cagcaaggaa ccgacatcgc
     2041 cgggcaagta gaggtcgttt gaaggcagat tctgccgcgc ataggtcaaa tgaaaacttt
     2101 gaatcggaga agcgatgaat acatcatggg ggaccaaagc ctcgcaccga cgaaagcggg
     2161 ccattcgtag aacgaaggtc cgctgggatc tgctcggact acaggacccg gaacagacgc
     2221 cgttgacgac gtcgatagac gatattcgcc gccagatcat ctcagcggca agccaaggcc
     2281 tgagcccgga tacgatctcc cgttctcacg caaaggctgg acgtcccaaa ccaggatcca
     2341 aggcgtcggt ggctacccgc aagcccggtg ccgcttcgca gtaggcttta cgtaagcaca
     2401 tcgcacccga aaggagggca tcatcggagg aacaggcaaa accacaagcg ctaagcggga
     2461 ccgtgagaaa tcgcgtcagc agaaacaacg cgccaaagaa gaacgccgtg tacagcggaa
     2521 ggccgccaag ctcaaccggc cgacacaaga aggaggggaa gaccctgacc ttgccggctt
     2581 gcgatggggg ccacagcctc cgctctattg atcagcaccg ccagatgcat ctattcgtcg
     2641 ttgattcgcc tctccgatct gtaccacctc tgcgtccttg aattgataat cacggtaggc
     2701 tgccagcgtg ggcgcaaggg agattttcaa ctccagacgc ttgggttttc cggtcgaggt
     2761 atagggtaca ttttgtccaa agcggatgac cttagggcat ttcgagaacg gaagccgttg
     2821 acggcagtgg gcgagtacct cggcttcggt gggtggagag acgccatccc ttggtactac
     2881 ataagcggca atctcctcgc catagtagcg atgttcgaac ggaacagtca tcgcgaatcg
     2941 gacaagaggg tgactcctca acacctcgtc gatttccaac ggagcaatat tgaccccgcc
     3001 tcgaataatt aattccttg
//
LOCUS       DATNDD010000201         4248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2443145, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000201 DATNDD010000000
VERSION     DATNDD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443145"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VN647_04860"
     CDS             complement(<1..465)
                     /locus_tag="VN647_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96397.1"
                     /translation="MDDSVAFRRTEPSGHTVTAGVLSKPGLELRIGSNIFRSTNGVVK
                     IHGKEQLVLEVKPERGLLLATLDLYNEHGIRLVHLRRNVLMLNDNERFTVDVLLGQGL
                     SSVDSPSILVTDLQLGHVVLEARMTSEHRVDLLCGKLYSHKGMPVEITPHYCR"
     gene            718..795
                     /locus_tag="VN647_04865"
     tRNA            718..795
                     /locus_tag="VN647_04865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:754..756,aa:Glu,seq:ctc)
     gene            945..1505
                     /locus_tag="VN647_04870"
     CDS             945..1505
                     /locus_tag="VN647_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96398.1"
                     /translation="MNLMTFRFRQSQAVDFYWSLELAKRGSLREFKVHPNITIYESTF
                     DLLNPIQIGHALSLAKALVLDKRAEVFTGNTSLTMVTVRDVLQCYQQSMQVDDHHAHC
                     WFKIRLTFDLTVTRFEVESDDLAKSFVFPCRRAATHAYGIHYEHPGTIYDQLNSALWR
                     AGTRWCPRLADLSEIKLVNVELQTKR"
     gene            1661..2107
                     /locus_tag="VN647_04875"
     CDS             1661..2107
                     /locus_tag="VN647_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96399.1"
                     /translation="MRPKHILTALAIIAVTVAVSVPNSLAGAWRLDVSDRHVVDRGEL
                     GWDPLIYTVGDMGDLPKHDPEYQTLGHPGHGPIPFTNRFSNQESGHTRETGSSSNESQ
                     SQGGHNPSDFLKGSPGSLTAPFSETIYGGVPSTPSADPSVRDLSRD"
     gene            2595..3701
                     /locus_tag="VN647_04880"
     CDS             2595..3701
                     /locus_tag="VN647_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HWG96400.1"
                     /translation="MYAREFRDGAKDGLEALEPLDPDKITSFSDLLEAMGKTAFGGRH
                     VGKAFDVLWAMIKDPDCHIVMTLSGAMTIAKMGKIITKMIDEGMVQCVISTGALMAHG
                     LSESVGKTHYRHDPSMSDEELFRKGYNRVYDTLEMEANLNYVEHIVAHTVKRLNHDRP
                     LSSEIITRELGKTLAEELDGDGILKSAYLKKVPIYIPAFTDSEVGLDMGTWAMAREVD
                     RARSQVGQGRDLDILRAIHQSCPSFNPYLDLNSYADHVLSAKRLGIFTIGGGVPRNWA
                     QQVGPYIEIGNLRLGLNVKPPRFHYGVRICPEPDYWGGLSGCTYQEGLSWGKFVTPKD
                     GGRFAEVLSDATVVWPLLMMGILEKKKAGMVRGS"
     gene            3698..>4248
                     /locus_tag="VN647_04885"
     CDS             3698..>4248
                     /locus_tag="VN647_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWG96401.1"
                     /translation="MKWLTGQRLLLVFMGSALLWSSPALAGNSLEADERVRGQANAPL
                     TLIEYSDFTCGYCLKFFKRTWPRIQARYVDTGKVRFVYRDFPRGDQGSGLDAAMAARC
                     AGAQGQYWAMHDRLFSEGGQLDKQVYLRHAAALSLDQAVFERCINDGRYTRAIFEDRQ
                     EANQWGFHGTPGFILVRTAAEPT"
BASE COUNT          984 a         1077 c         1202 g          985 t
ORIGIN      
        1 ccggcagtag tgcggagtga tctctaccgg cattcccttg tgcgagtaca actttccgca
       61 gaggagatcg actctatgct ccgatgtcat tcgtgcttca agcacgacgt ggcccaactg
      121 cagatctgtc accagaatag agggactgtc aaccgacgaa agaccttggc ccagcagaac
      181 atcgacggtg aatcgttcgt tgtcgttcaa catgagaacg tttctacgca ggtgtacgag
      241 tcggatgccg tgctcgttat agagatcgag ggtggcgaga agcagtcctc gttccggttt
      301 gacctcaagg acgagttgct ctttcccatg aatcttgacg acgccattgg tgctgcgaaa
      361 aatattcgaa ccgatacgga gttcgagtcc cggcttggaa aggacgccag ccgttaccgt
      421 atgaccagag ggctcggttc tgcgaaacgc caccgagtca tccattttaa caacctcgaa
      481 ccatgaggtt cttaggtcgt gtggaggtgc aaggcatatg gagcttcatt aaaacgttag
      541 ttaggctagg cgttccgctc tgcccctgtc aagcacgtcc aatgcggaaa gccatatatt
      601 atggctccgt gcaaacggcc taatgggacg atcccaaagt gctctccttc cggaggattg
      661 gcgcttcgtt gcaaacggaa aaccgcttat gctacggtcc actcctcggt ctggaatggt
      721 cccatcgtct agcctggcct aggacggagc cctctcaagg cttaaacacg ggttcgaatc
      781 ccgttgggac caccacactt ttccttccct gggaggccta gcgaaggtcc agtttccgtc
      841 aaagaaggag ctgaaaatgt tgcagaagtt ggcgtagatt aactgtgacc ggatggcttc
      901 ctgaaggttc caaatccagt aatcttagtg acgggggttg aatcatgaac ctgatgacct
      961 ttcgcttccg ccagtcccag gcagtggact tttattggag cctcgagttg gctaagcggg
     1021 gatctctacg cgaattcaag gtacatccca acatcaccat ttatgagtcg actttcgacc
     1081 tcctgaaccc gattcaaata ggacacgcct tgtctctagc aaaggccttg gtgctcgaca
     1141 agcgagccga agtcttcacg gggaacacgt cgttgacgat ggttaccgtc cgtgatgtgt
     1201 tgcagtgcta tcaacaatcg atgcaggtcg acgaccatca tgctcattgt tggttcaaga
     1261 tccggctcac atttgacctg accgtgacac gattcgaagt ggagtccgac gacttggcga
     1321 agtcgttcgt gttcccgtgt cgacgtgcgg ccacacatgc ctatggcatt cactatgagc
     1381 acccagggac gatctacgac caactgaact cggccttgtg gcgtgccggg acacgttggt
     1441 gtccgcggct cgcggatctc tcagaaataa aactcgtcaa tgtggagttg cagactaaaa
     1501 gataaagaac cgctgcgctt cagctcatgc gacagccggt ctaagagggc tctgtgaact
     1561 gcaggttgcg caggtgtcct cttgtagtcg tgcaatgtcg tgcgttacga tgtcatggta
     1621 gaagcaggca gtttaacgat acgtgaataa ggaggatgtt atgaggccga aacacatatt
     1681 gacagccttg gcgatcattg cagtaaccgt agcagtcagc gttccgaata gccttgccgg
     1741 ggcttggcgg ctggacgtca gcgaccgaca tgtagtcgac cgaggcgagt tagggtggga
     1801 cccgttgatc tacactgtgg gcgacatggg tgacctcccc aagcatgacc ctgagtacca
     1861 gacattgggg caccccgggc acgggcccat ccctttcaca aatcgtttct caaatcagga
     1921 atccggccat acacgggaaa ctggttcatc cagcaatgaa agtcaaagtc aaggtggcca
     1981 caacccctct gactttctga aaggatcgcc tggatccctg actgctccct tcagtgaaac
     2041 tatttatgga ggggttccgt cgacgccgag tgcggatcca tcggtcaggg acttgagccg
     2101 tgactgaccc atcaagaagg tagaggtgtg actgcacgag gggagggact tcgctccaac
     2161 ctgacgccaa tgtcatcatt tttccgattc ctcccgttca acgagataga cgtgggagtc
     2221 atagctcctc agaagctgca cgtgatggca aacctatcct cctcacggag tttcgatatt
     2281 caatctcgcg tgcttatgct gtccgtctct tactcttaat tgaggcggac ggacaaacat
     2341 ctccggaggg tattcattcc taatgcaaaa ctcgcctgac ccggagccgc tccagcgatc
     2401 cactgtttga tgctgcagtc tgcggacagc agttcaattc gctaatgcca cttcgggtac
     2461 gagttatcaa gaaatccact cataaaaatt cttcgacctc ctgaagtctt ccgtacgacc
     2521 gaacctttca ttgacgcctc tattcttggt tgtgtataaa caaacgaaca ctttggagtt
     2581 tctcaaagga tcgtatgtac gcacgcgagt ttcgtgacgg cgcaaaagac ggattggaag
     2641 cgctcgaacc gcttgatccc gacaagatca cctcgttcag cgatttgctt gaggccatgg
     2701 gcaagacggc gttcggaggt cggcatgtcg gcaaggcgtt cgatgtgctc tgggcgatga
     2761 tcaaggatcc tgactgccac atcgtgatga cgctgtccgg tgcgatgacg atcgccaaga
     2821 tgggcaagat catcacaaag atgatcgatg aaggaatggt gcaatgcgtc atctcgaccg
     2881 gggcgttgat ggcccatggc ctcagtgagt cggtcggcaa gacgcattat cggcacgatc
     2941 cttcgatgag cgatgaagag ctctttcgga aagggtacaa tcgcgtctac gacacgctcg
     3001 agatggaagc gaatctgaat tatgtggagc atatcgtcgc gcacacggtc aagcgcctca
     3061 atcacgatcg gccgctttca tcggaaatca tcacgcggga gttgggaaaa acgttggcgg
     3121 aggaattgga cggcgatgga attctcaaaa gcgcgtactt gaaaaaggtg ccgatctata
     3181 ttccggcttt cactgattcg gaagtggggc tggacatggg aacctgggcg atggctcggg
     3241 aggttgatcg agcccgatca caggtggggc aggggagaga tctggacatt ttacgggcga
     3301 tccatcaatc gtgtccttcc ttcaatccct atctcgatct caacagctat gccgaccatg
     3361 tcctctccgc caaacgactt ggaattttca cgatcggcgg aggtgtccca cgaaactggg
     3421 cgcaacaggt cggcccgtac attgaaatcg ggaatcttcg gttgggcctg aacgtgaagc
     3481 ctcctcgatt tcactatgga gtgagaattt gccccgaacc ggactactgg ggtggactca
     3541 gcggctgtac gtatcaagaa ggactgtctt ggggaaaatt cgtgacaccg aaggacggtg
     3601 ggcgatttgc cgaagtgttg agcgatgcga cggtcgtctg gccgttgtta atgatgggga
     3661 ttctggaaaa aaagaaagcc ggtatggtgc gtggttcatg aagtggttga ccggacaacg
     3721 gttgcttctg gttttcatgg gcagcgccct cctgtggtct tctcccgctt tagccggaaa
     3781 ctccctcgaa gctgatgagc gggtacgagg gcaagccaat gcacccctga ccttgatcga
     3841 atactctgat ttcacatgcg ggtattgttt gaaatttttc aaacggacgt ggccccgaat
     3901 tcaggctcgg tacgtcgata caggaaaagt gcgatttgtc taccgagact ttcccagggg
     3961 cgaccaaggg tctgggttag acgcagcgat ggcggcacga tgtgccggag cacaaggcca
     4021 gtattgggcg atgcatgatc ggttgttttc ggaaggcggt cagctggaca agcaggtgta
     4081 tcttcgacat gctgcggccc tgagtttgga tcaagccgtg tttgaacggt gtataaatga
     4141 cgggcggtat acgagggcga ttttcgaaga tcgccaggaa gccaatcagt gggggtttca
     4201 tggaacccct gggttcattc tcgtgcgaac ggccgccgag ccgacgga
//
LOCUS       DATNDD010000202         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_268116, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000202 DATNDD010000000
VERSION     DATNDD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268116"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VN647_04890"
     CDS             <1..584
                     /locus_tag="VN647_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWG96402.1"
                     /translation="EGWFFHSPGLKLVAPSTPYDAKGLLKAAIRDPNPVIYFEHKFLY
                     RRIKSPIPKEDFVVPLGKADVKRAGNDISVITYGAMVHLALEAAQTLADEGIDLEVVD
                     LRTLIPFDKEAIYSSVRKTSKAILLHEDNKTGGIGAEIAALLAEDCFDCLDGPIVRIA
                     PPDTPVPFSTPLEEFFLPKTSDIVSAARKLAAY"
     gene            684..1826
                     /locus_tag="VN647_04895"
     CDS             684..1826
                     /locus_tag="VN647_04895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWG96403.1"
                     /translation="MATDIIMPQLGESIAEGTVVKWLIPVGGMIQKDESLLEVETEKV
                     TLEIPSPATGMLSETIVHEGQTVPVGTLLARIDSAPPSEVVNRVGGVVVRPMEPTSAG
                     DQHHSPAVRQLATEHGIDLSQVKGTGVGGRVTKKDVLDFIAQSGSQTKGAAIAGEPSM
                     GEELLLFTQMRKTIADRMVKSKQASAHVTTFFEVDFSSVTKFRQGRSLTYLPFVVRAV
                     TRAIRDVSIVNSSWGEQGILVKKDIHMGIATALEDGLLVPVVRYADRKGLTQLAKEIA
                     DLSERARSKKLNPEEVQGGTFTITNHGGFGSLFSTPIIHQPQIAILGIGAIQKRAVVI
                     DDAIAIRPMGYLSLSFDHRVIDGATADQFTAKVKHYLEQSHWEQIL"
     gene            1823..>2385
                     /locus_tag="VN647_04900"
     CDS             1823..>2385
                     /locus_tag="VN647_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWG96404.1"
                     /translation="MNRTTRAVIVSAVRTPMGSFNGMFSQVPATKLGSLAIAEALKRA
                     HVPPDRVDQVYMGCVLSAGLGQAPARQASIGAGISHSIGATTVNKVCGSSLQTAIMAY
                     QAIMLGEASIVVAGGMENMTRAPYLLEKARQGYRLGHAELVDSLVKDGLWDVYNHFHM
                     GNGGERCAAKYRLTRTELDDFALESYRR"
BASE COUNT          553 a          653 c          703 g          476 t
ORIGIN      
        1 ccgaaggctg gttcttccat tccccgggac tcaagttggt cgccccgtcc acgccgtacg
       61 acgccaaggg gctcctcaag gccgcgattc gcgaccccaa cccggtgatc tactttgagc
      121 acaaatttct ttaccggcgc atcaaatctc cgatacccaa agaagacttc gttgtgccgc
      181 tcggaaaggc cgatgtgaag cgggccggta acgacatctc cgtgatcacc tacggcgcaa
      241 tggtccatct ggcgctggaa gccgcccaga cattggccga tgagggaatc gatctggaag
      301 tggtcgactt acgcacgttg atcccgttcg acaaggaagc aatttattcg tcggtgcgta
      361 aaaccagtaa agccattctt ctgcacgagg acaacaaaac cggcggcatc ggggctgaaa
      421 tcgccgcgct gttggcggaa gattgtttcg attgcttgga cgggccgatt gtgcgtatcg
      481 cgccgccgga cacgccggtg ccgttcagca cgccgctgga agagtttttt ctcccgaaaa
      541 caagcgacat cgtctcggcg gcgcgaaaac tggcagcgta ctgaaaagga ctgaggactg
      601 agttatgagg actgagaacc aaagaaccgc acatttgcta aatgcccact cagttctcag
      661 cactcgtcac tcagcacttg agtatggcca ctgacatcat catgcctcag cttggagaga
      721 gcatcgccga agggacggtc gtgaagtggc tcatccctgt cggagggatg attcaaaagg
      781 acgagtcgct cttggaagtc gaaaccgaaa aggtgacatt ggaaattccc tcgcccgcta
      841 cgggcatgct gagcgaaacc atcgttcatg agggacagac ggttcctgtc ggcactttgc
      901 tggcgcgcat cgacagcgct ccaccatcgg aagtcgtcaa ccgagtcggc ggagtcgttg
      961 tccgtccgat ggaaccgact tcggcggggg accaacatca ctcgcctgcc gtccggcagc
     1021 tggcgactga gcatggaatc gatctttcgc aggtgaaggg gaccggggtc ggaggcaggg
     1081 tcaccaagaa ggatgttctc gactttatcg ctcagagcgg ttctcagaca aaaggtgccg
     1141 caatagcagg cgaaccttcc atgggcgaag agcttctcct cttcacgcag atgcgtaaga
     1201 ccattgccga tcggatggtc aagagcaagc aagcttcggc acatgtcacc accttttttg
     1261 aggtggactt ttcgagcgtc accaaattcc gacagggccg cagcctgacc tatttgccgt
     1321 ttgtcgtccg tgccgtaacc agagccattc gagatgtctc gatcgtgaac tcgtcatggg
     1381 gcgagcaagg catcctggtc aaaaaggata tccacatggg aatcgccacg gcactcgaag
     1441 acggactctt ggtgccggtg gtgcgatatg ccgaccgaaa aggactgact cagctggcaa
     1501 aagaaatcgc ggatctttcg gaacgggcca ggtccaagaa gttgaacccc gaagaggtgc
     1561 aaggcggaac gttcaccatc acgaatcatg gggggttcgg cagcttattc agcaccccga
     1621 tcatccatca accgcagatt gccattctgg gtatcggtgc cattcagaaa cgagccgtcg
     1681 ttatcgacga tgcgatcgcc attcggccga tggggtacct cagcttgtcg ttcgatcacc
     1741 gggtcatcga tggggcgacg gcggatcagt tcacggctaa ggtgaaacat tatctcgaac
     1801 aaagccattg ggagcagatt ctgtgaaccg gacaacacgc gcagtcatcg tcagcgccgt
     1861 acggacaccc atgggcagct tcaacggcat gttcagccag gtgccggcga caaaattggg
     1921 cagtcttgcc attgccgaag cgttgaagcg ggctcatgtg ccgcctgatc gtgtggacca
     1981 ggtctacatg ggctgtgtcc tcagcgccgg tctgggccag gcgccggccc gacaggcgtc
     2041 gatcggagcc ggaatttccc attcaatcgg agccacgacg gtgaataaag tctgcgggtc
     2101 cagcctacaa acggcgatca tggcatacca agccattatg ctcggagagg cgagcatcgt
     2161 cgtcgctggt ggaatggaaa acatgacccg cgcgccatat ttgttggaga aagcgcgaca
     2221 gggttatcgg ttaggacatg ccgaattggt cgacagtctt gtcaaggacg gactgtggga
     2281 cgtctacaat catttccaca tgggaaacgg cggtgagcgc tgtgcggcca agtatcggct
     2341 gacgagaaca gaactggatg atttcgcact cgagagctat cggcg
//
LOCUS       DATNDD010000203         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3461379, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000203 DATNDD010000000
VERSION     DATNDD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461379"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VN647_04905"
     CDS             <1..718
                     /locus_tag="VN647_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96405.1"
                     /translation="SNAAEIDRLALALAKEPGSKVFISLAEEYGKAGMWEEAVAVLED
                     GLKTYPGFITAMVALGRAYEQLNQPVKAKAILEEAIKSSPDNLRAHRILAKLYGAQGA
                     KEAAIRSCNVILSINPHDQEALSLRSGLDVPTTHGAAQSQGQLDEQPVKASSLDSVSL
                     REKGMTSVLGDATLPTKDKANGLSRNLPRAEEKPVPTGGSTAQTSQKTRSPVIVQLEQ
                     WLKSVRTRRQDPQASSRSRS"
     gene            821..1294
                     /gene="aroQ"
                     /locus_tag="VN647_04910"
     CDS             821..1294
                     /gene="aroQ"
                     /locus_tag="VN647_04910"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWG96406.1"
                     /translation="MLRILVLHGPNLNLLGHREESIYGTVTLDTIDASLIKLGSELGT
                     ELVIRQSNLEGELVTWIQEAQRGYQGIIINPAAYTHTSIAIRDALAAVDLPAIEVHLS
                     NIYRREEFRHHSYVSGVVLAQIAGFGATGYLLALRGLCEHLSVAGSKGFSGGRTR"
     gene            1381..1941
                     /gene="efp"
                     /locus_tag="VN647_04915"
     CDS             1381..1941
                     /gene="efp"
                     /locus_tag="VN647_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWG96407.1"
                     /translation="MISTVDFRSGVRLMVEGEPFYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGERFDEPDLEERDMQFLYASGDSYTLMDTETYEQLTFERSQLGGNA
                     DLLKENMIVKILIYEHRPIAVELPNFIELRVVDAEPGVRGDTASGGTKPAVVETGATI
                     KVPLYLEVGTVIRIDTRTRSYVERVR"
     gene            1938..2501
                     /gene="accB"
                     /locus_tag="VN647_04920"
     CDS             1938..2501
                     /gene="accB"
                     /locus_tag="VN647_04920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HWG96408.1"
                     /translation="MNRRRSVHSKAKTRRIVLPETANETRHEASLTGGSTKQIQELID
                     LLRRNNLTELEFERQGIRIRVRHELVARPLATSAQESVPILAQQPMHSASATASVSDA
                     STGFVTVTSPIVGTFYRSPSPDADPYVDEGESVKKGQVLCIVEAMKLMNEIESEVDGR
                     LVKILVENTKSVEYGQALFLIDPKAAS"
     gene            2545..>2733
                     /locus_tag="VN647_04925"
     CDS             2545..>2733
                     /locus_tag="VN647_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWG96409.1"
                     /translation="MFKKVLIANRGEIALRVIRACKELGIKTVAIHSEADALALHVRA
                     ADEKVCVGPAESALSYRNI"
BASE COUNT          674 a          683 c          758 g          618 t
ORIGIN      
        1 cagcaatgct gcagaaattg atcggctggc cttagcctta gctaaggagc cggggtccaa
       61 ggtatttatt tctctggcag aagaatacgg caaagccggc atgtgggaag aggccgttgc
      121 cgttcttgaa gatgggttga aaacctatcc aggtttcatc accgccatgg tggcgttagg
      181 tcgggcatat gagcagctga accaaccggt caaggctaag gccattctcg aagaagctat
      241 taaatcaagt cctgataatc ttcgtgcaca tcgaatattg gccaagctct atggagctca
      301 aggggccaaa gaagcggcta tccgatcgtg caatgtcatt ttatccataa acccacacga
      361 ccaagaagcc ttatctcttc gttcagggct cgacgtaccg acgactcatg gcgcggcgca
      421 atcgcaagga caattggacg agcaaccggt gaaagcttca agccttgact ctgtcagcct
      481 tcgtgaaaag ggtatgacat ccgtcttagg cgatgcaacg ctgccaacga aagataaagc
      541 caacggcttg tctaggaacc tacctcgcgc agaagaaaaa cctgttccta cgggcggttc
      601 cacagcccaa acctcacaga aaacgagaag cccggtgatc gtacagcttg agcagtggct
      661 caaatcggtc cggacacgtc gacaagatcc ccaagcctct tctcgctccc gctcatgaac
      721 ttccccatga cttcaatggt ttgaccctct gagaacggac tgctagaatg ccgcccttgc
      781 cgtggtgatg taagcaggtg taaagggagg tatcgcacac atgttgcgca tcctggtcct
      841 tcacggacca aatctgaacc tgttgggtca tcgagaagaa tccatttatg ggacggtgac
      901 ccttgatacg atcgatgcat cgctgataaa gctgggtagt gaacttggga ctgagcttgt
      961 tattcgccag tcgaatcttg aaggagaatt ggtgacctgg attcaagaag cgcagcgagg
     1021 ctaccaaggc attatcatta atccagcggc ctatactcac accagcatcg cgatacgcga
     1081 tgccctggcc gccgttgatt tgcctgccat cgaggtccat ctgtcaaaca tctatcggcg
     1141 tgaagaattc aggcaccatt catatgtgtc gggggtcgtg ctggcacaga tcgccggatt
     1201 cggtgcaacc gggtatctat tggccttgcg tggactgtgc gaacacctat ctgtcgccgg
     1261 atcgaagggg ttttctggtg ggcgtactcg gtaagcggaa cgaaggcagg ggcgcaccga
     1321 gggtatcacc agccggttgt ttgcttaagt tgtttgttta aaaagagaag ggagttgcga
     1381 gtgatttcca cagtggattt tcggagcggt gtacgcttga tggtcgaagg cgaacccttt
     1441 tatatcgtcg agtttcagca tgtaaagcca ggcaaaggtg gtgccttcgt acgtacgaag
     1501 ttgaaaagtt atctctccgg aaacgtactg gaccgaacgt ttcgttcagg agaacggttt
     1561 gatgagcccg atctggaaga gcgcgatatg cagttccttt acgcatccgg agattcctat
     1621 accctcatgg atacagagac gtacgagcaa ttgacgtttg agaggagtca gttgggaggg
     1681 aacgcagatt tattgaagga aaatatgatc gtcaagatcc tcatctacga gcatcgcccg
     1741 attgccgtcg agttgccgaa cttcatcgag ctcagggtcg tcgatgcgga gccaggcgta
     1801 cggggagaca ctgcctctgg aggaaccaag ccggcggtcg tggaaacggg agcgacgatc
     1861 aaagttccgc tttatttgga agtgggcacc gtcatccgga tcgatactcg caccagatca
     1921 tatgtggagc gtgttcggtg aaccgtcgtc gatctgttca ttccaaggcc aagactcgtc
     1981 ggattgtgtt gccggagact gcgaacgaga cgagacatga agcctcgctc acggggggat
     2041 ccaccaagca aatccaagaa ctcatcgatc tgcttcggcg caacaatttg accgaacttg
     2101 agtttgaacg acaaggcatc cgtatccgtg tccgtcatga actggtagcc agaccgctcg
     2161 cgacttcagc gcaagagtct gtcccaatcc tggctcaaca accgatgcat tccgcttcag
     2221 ccacggcatc agtgtcggac gcgagtacgg gttttgtgac cgtcacttcc cccatcgtcg
     2281 gcacgtttta ccgatctccc tcaccggacg ccgatccgta cgttgacgag ggggagtccg
     2341 tgaaaaaggg gcaagtgctg tgcatcgtcg aagcaatgaa actcatgaac gaaattgaat
     2401 cggaagtgga cggtcgtctt gtcaagatct tggtggaaaa tacaaagtca gtagaatatg
     2461 gtcaggcact gttccttatc gaccccaaag ccgcttcata ggtcattcta aaggctgctc
     2521 tcgccatcgt atcggagccg agacatgttt aagaaagttc tgattgccaa tcgcggagaa
     2581 atcgcgttgc gggtgatccg cgcttgtaag gaactcggca tcaagaccgt ggccattcat
     2641 tctgaagcgg atgcgcttgc actgcacgtt cgagcggccg acgaaaaggt gtgtgtcggg
     2701 ccggccgaat cggcattgag ttaccgaaat att
//
LOCUS       DATNDD010000204         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1399786, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000204 DATNDD010000000
VERSION     DATNDD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399786"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VN647_04930"
     CDS             complement(<1..791)
                     /locus_tag="VN647_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG96410.1"
                     /translation="MSRISNERMEKALEQLKTISEKDYAKVKNMANIVRPLRSVLFKT
                     PDDYSMTGWKDLVIPSDDGTPLEAWYIPARGGDSDKVVIFNHALPMCRAGFPGHLGEP
                     WSMYDAVEIDFVIQYKHLTDAGYNVLTYDIRNHGNSSAANGGLSGIGQWEWRDCVGVK
                     KYVDSHPSLSKMKVGLYSQCMGGNSQYHAIHRRPDLFENVRCMVSPMVVSMAAIYDAF
                     SELQGVRQYQELIDLELLKVGAFVAAEMTPHLWAASVKMPVLMVQV"
     gene            1083..2021
                     /locus_tag="VN647_04935"
     CDS             1083..2021
                     /locus_tag="VN647_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWG96411.1"
                     /translation="MLPGLHALRKGIARWTDKGDQLTTAISGLSLFWRDTPTSPMSYM
                     YERSVCLIAQGAKQVLLGDDVYVYDAHHFLITSVDLPVICQVLKASREQPYLSLLLKL
                     DRHEMSQLIADSNLPPPPAQQSGRGMAIGQVTLPLLTAFQRLLDLLAEPKDIPILAPI
                     VQREIMYRLLIGDQGMHLRQMASAGSQSQQITQAICWLKSNFTRSFRIEELAGRVNMS
                     PSTFHHHFRTLTAMSPLQYQKWLRLNEARRLMLTDRLDATTAAFQVGYESPSQFSREY
                     SRSFGAPPLRDITNLRTSADNVVRKASRPSAEGLRG"
     gene            complement(2106..2345)
                     /locus_tag="VN647_04940"
     CDS             complement(2106..2345)
                     /locus_tag="VN647_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG96412.1"
                     /translation="MLAAIDDRHRLGAVTAKDVMTANPYSVKPETTLGTLVHVLRASD
                     LVRVPVVNAKDKLVGIIARRDVLRTYLATGGKREQ"
BASE COUNT          545 a          710 c          644 g          618 t
ORIGIN      
        1 acctggacca tgaggacggg catcttcacg ctggcggccc acagatgcgg ggtcatttcg
       61 gcggcaacaa acgcacccac cttgagcagt tccagatcga tcaattcctg gtattgtctc
      121 actccctgca attccgagaa ggcatcgtag atcgccgcca tggagacgac catgggactc
      181 accatacatc gcacgttttc gaagaggtcg ggacgacggt gaatggcgtg atattgcgag
      241 ttgcctccca tgcactggct gtagagcccg actttcattt tgctgagcga aggatggctg
      301 tcgacatact ttttgactcc cacgcagtcc cgccactccc attgcccgat gccactgagc
      361 ccgccgttcg cggcgctgct gttgccgtga ttgcggatgt cataggtgag cacgttgtag
      421 ccggcatcgg tcagatgctt gtactgaatc acgaagtcga tctcgactgc gtcgtacatg
      481 ctccacggct cacccagatg tccgggaaag ccggcgcgac acatcggcag agcgtggtta
      541 aaaatgacca ccttgtcgct atcgccacct ctggcgggga tgtaccaagc ttccagcggg
      601 gtgccgtcgt cggaaggaat gacaagatct ttccagccgg tcatgctgta atcgtccggg
      661 gtcttgaaga gcacgcttcg gagcggcctg acgatattgg ccatattttt gactttcgca
      721 taatcttttt cggaaatagt cttaagctgc tcaagcgcct tttccattct ctcattggat
      781 attctgctca tgactcacct ccatgtgatg tttagtttta gtggacgatg ggtggttctc
      841 acatgttgcg gctcccatta ctttgtcgat tctgctcaca tcaaacgaca tgccgtctgg
      901 ttgataatca tcttgtgctc ccacgcccac tggtcggtag cctgatcctg ctcatttctt
      961 gcctaatcct acgaggttcg tgaacggatc gcttgtaccg acatgtgagg tctggtagcc
     1021 ttcaaagggt gaaccaaatg ccaaaatcgg atcgtctcaa gcaggagtct tcgaggacta
     1081 agatgctacc gggccttcac gctttgagga agggtatcgc gcgatggacc gacaaaggcg
     1141 accagctcac aaccgccatt tctggattgt cacttttttg gcgagacaca cccacttcgc
     1201 cgatgagtta tatgtacgag cgcagcgttt gcttgatcgc ccagggagcc aaacaggtgc
     1261 tgctcggaga tgatgtgtat gtgtatgacg cgcaccattt tttgatcacg tctgtggatc
     1321 tacccgtcat ttgtcaagtt ctcaaagcaa gccgtgagca gccttacctg agtctcctct
     1381 taaagcttga tcgacacgag atgtcccagt tgatcgcgga cagcaatctt ccaccgccgc
     1441 ctgcgcagca atccggccgt ggaatggcca ttggacaggt cacattgccg ctactcaccg
     1501 cctttcaacg attgctcgac ctccttgccg agccaaaaga cataccgatt ctggcgccga
     1561 tcgttcagcg ggaaatcatg taccgtttac ttataggcga tcaaggcatg catctgcgcc
     1621 aaatggcgtc ggcaggaagt cagagtcaac aaatcacgca agccatctgt tggttgaaga
     1681 gtaatttcac gaggtcgttc cgcatcgagg aactcgccgg tcgcgtcaat atgagccctt
     1741 ccacctttca ccatcatttc cgaacgctga cggcgatgag tccactgcag tatcaaaaat
     1801 ggttgcgatt gaacgaagca aggcgcttga tgctcactga tcgtctggac gcgaccaccg
     1861 cggcctttca ggtcggctat gaaagtccct ctcaatttag tcgagagtac agccgctcgt
     1921 tcggggcgcc tccgttgcgc gatattacaa acttacgaac gagtgcggat aatgttgtcc
     1981 gcaaagcgag caggccttcc gccgaaggat tgagaggtta gtgactacgc tacccgacat
     2041 cagtcctgag agcgttgaac tgctttaaca tctaatctcc taagtcggtt tgtctcataa
     2101 gctccttact gttctcgttt tcccccggtg gcaagatagg ttcgcaagac atcgcgtctt
     2161 gcgatgatgc cgactaattt atccttggca ttcacgacag ggacacggac cagatcgctt
     2221 gcgcgcagca catggaccaa cgttccgagc gtcgtttcag gcttgaccga gtaggggttg
     2281 gcggtcatga cgtccttcgc cgtgacggca ccgagccgat gacggtcatc gatggctgca
     2341 agcaaatcat gttcgctgac gatccccgat cagtttccgc ccattctcca cgaccggcac
     2401 ggccccgaac ccctcgatca tcaatgaagc aatcatgtct cccttcgtct tcaggcggat
     2461 acattgcact tctttctcca tcacctggct gaccgtcatg gaatcaaagt ctttgac
//
LOCUS       DATNDD010000205         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1687167, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000205 DATNDD010000000
VERSION     DATNDD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687167"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VN647_04945"
     CDS             complement(<1..200)
                     /locus_tag="VN647_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96413.1"
                     /translation="MMKLDPKIPPGPLETKWDRRRFSEKLVSPNNKRKITVIVVGTGL
                     AGAGAASTLGQLGYQVECFCFQ"
     gene            complement(197..877)
                     /locus_tag="VN647_04950"
     CDS             complement(197..877)
                     /locus_tag="VN647_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HWG96414.1"
                     /translation="MPRPSNHFQSTVGSKVVMALSGLGLVIYVIFHMLGNLQVFEGSH
                     ALNAYAAFLRDMPILLWTARIGLLSLAALHIVPAIRLTLRNRHARPTAYAVREYRQAS
                     FASRTMAASGFVLLFFIIFHLLHLTAGVIDPSAVDRLDVEGHRDVYGKLVHAFQNPFI
                     AALYLTGQLGLGLHLSHAVTSSVQTLGLEHAAFNRLFKAAGPTVALVVVLGNVAIIMA
                     IFLGIVRG"
     gene            1424..2353
                     /gene="lpxC"
                     /locus_tag="VN647_04955"
     CDS             1424..2353
                     /gene="lpxC"
                     /locus_tag="VN647_04955"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWG96415.1"
                     /translation="MRNQQTLASAVTCSGVGLHSGQSASITLRPAPPNTGVVFVSRRT
                     DVDTYLSASIEHRVPTELCTAISGNGFQVQTIEHLLSALSGLHVDNVFIDVSASEVPV
                     MDGSAAPFVRLIQSVGIVSQERKQPFLKIMAPIEVTEGSKRVRIEPSSTPRITYSIHY
                     EHPLIRTQTYAYDCSVNAFENEIAEARTFGFLYEVQALWARGLGKGGTLDNTIVLSDD
                     GVVNESGLRFDDEFVRHKILDLIGDFSLLGAPFIGHIVADRSGHALHTRLVQQILTQT
                     DKWVLLNIESSGAEKRSAAHALHLQPAVALQAS"
     gene            complement(2572..3087)
                     /locus_tag="VN647_04960"
     CDS             complement(2572..3087)
                     /locus_tag="VN647_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWG96416.1"
                     /translation="MEELTDILVGLEQRINAYKSRFQELEKKRRRLDDEIATIKKYLE
                     LAETLYRVEADKAKLASLSNQIITTDDSKGIRPVPVMDVTDQSREILLGRSKYVGKSV
                     PQAAYEILRESNRAMHAKELVQRLIEGGLQIKGKTPLTSIATSLKRDKRFRKVGPNTF
                     EALDDMLIQAV"
     gene            3274..>3947
                     /locus_tag="VN647_04965"
     CDS             3274..>3947
                     /locus_tag="VN647_04965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWG96417.1"
                     /translation="MLQSGVSIPHEPISRDALLEAAVAAGQEAGTLLLEYAGAGFHIE
                     YKNPINLVTDADRAAEQCVIKRIRAHFPTHRFLAEEQGRIEKAQSPYLWIIDPLDGTT
                     NFAHGYPTYCVSIGLEYEGRCVLGVVFDPTRNDLFTAIEHRGAQLNGRPIHVSNTMGL
                     DGSLLVTGFAYDIRETPRNNLDHFSNFALKTQGLRRTGSAALDLCYVAAGRFDGFWEV
                     RLNPWD"
BASE COUNT          883 a         1040 c         1026 g          998 t
ORIGIN      
        1 tcctgaaagc aaaagcattc cacctgatag ccgagctgtc ccaaggtcga agccgcgcca
       61 gctcccgcca ggccggtgcc gacgacgatc accgtgattt tccgcttgtt attcggactt
      121 accaattttt cgctgaaccg tcgccggtcc catttggttt ccagtgggcc gggagggatt
      181 ttcggatcaa gtttcatcat ccgcgtacga ttcccaagaa gatggccatg atgatggcga
      241 cattgccaag aacgaccaca agcgcaaccg tgggaccggc ggccttgaag agtcggttga
      301 atgcggcatg ttccagcccc aatgtctgta cgctgctggt gacggcatgg ctcaggtgca
      361 agcccagccc cagctgaccc gtcaagtaga gcgccgcaat gaacggattc tgaaaggcat
      421 ggacgagctt cccatagaca tcacgatgtc cctcgacgtc gagacgatcg acagcagagg
      481 gatcgatcac gccggccgtc agatgcagca ggtgaaatat gatgaagaac aagagcacaa
      541 aacctgaggc ggccattgtg cgggacgcaa aggatgcctg gcgatattcg cggaccgcat
      601 aggcggtcgg acgagcatgg cgatttcgca aggtcaaccg gatcgccggt acgatatgga
      661 gcgccgcaag gctcaacaac ccaatccggg ctgtccagag gaggatcggc atatctcgaa
      721 gaaacgccgc ataggcattg agggcatgag atccttcgaa tacttgtagg ttccccaaca
      781 tgtgaaaaat aacatagatc acgagcccca gcccgctcag agccatgacg actttactgc
      841 cgactgtgga ttggaaatgg ttagacgggc gtggcatccg cgggcattat acactgcttg
      901 aattacgaag aatcatgaga acgttggaga cgtgtctgcg tgtctgatgg acgaaggaac
      961 agcggctttg tggtgtcgtg cttgctgatt tgtcgggcgc aagaatgtga tcgagcatgc
     1021 ggcaatcgct tgacttggaa ctctcattgt tcgaaaggat ttattcccgt gaccagtcac
     1081 gtcgaacgcc atgacggaat tccgccttaa cctgctttct acaggatggg tgcaacgtca
     1141 tgggacggtg ccgatcgctt ccgcgtgttc cgagtgatgt ggacactgtt tgtccggtgt
     1201 cacacccccc atcggatctt cattttgtcg ctgaaccctt cttgaaatca cagttttctt
     1261 gaaaaacaca gttgtgcttt ttaagataca gttatatttc taaatattaa ttatatttgc
     1321 atgtaatatc atatacttat aataattaca ctttggcaca cgaattgctt ggctgtaatc
     1381 cgaatgactg tgtttgagca tttgtctgta tgagggagga cttgtgcgca atcaacaaac
     1441 tttagcatcg gcagtaacct gctctggtgt gggactccat tctggccagt cggcgtcgat
     1501 tacgctgcgg cccgcccccc ctaacaccgg tgtggttttt gtcagtcgta gaactgacgt
     1561 tgatacgtat ctttccgcct ccattgagca ccgggtcccc accgaattgt gcactgccat
     1621 cagcggtaat gggttccaag tgcagaccat agaacatctg ctatcggcgc tgtccggcct
     1681 tcatgtcgac aatgttttca tcgatgtgtc ggcaagcgaa gttccggtca tggatggaag
     1741 cgccgcgccg tttgtccggc tgattcagtc cgtcggcatc gtctcccagg agcgaaagca
     1801 gccgtttctt aaaattatgg ccccaattga ggtgactgag ggatcgaagc gtgttcggat
     1861 cgaaccgtcc tccacaccga ggatcaccta ctccattcat tacgagcatc ctctgatcag
     1921 aacgcagacc tatgcgtatg attgctccgt caacgcattt gagaatgaga tcgccgaggc
     1981 caggaccttt ggatttctat acgaagtcca agcattgtgg gctcgtgggc tcggcaaagg
     2041 aggaaccctg gacaacacca ttgtgttgtc ggatgacggc gtcgtcaatg aatctggcct
     2101 tcgattcgac gatgagtttg ttcgccacaa gattctcgat ctgatcggtg acttttctct
     2161 tttgggggcg cccttcatcg gtcacattgt cgcagaccga tcaggacatg cactccacac
     2221 tcggttggtc cagcagatcc tgactcagac ggataagtgg gtgcttctga acatagaatc
     2281 ctcaggagca gagaaacgat cggctgctca cgcgcttcac cttcagccgg ccgttgcact
     2341 tcaggcttct tagcctatag ggattttcgg tatccggctg attgccgttc tttccggtag
     2401 gacgctcttc tcatccagaa catcacagca tgatacgccg ggggtaagtg gcctcacccc
     2461 cggcgtatca atcatggcgg ggattacttc ttcttcttct tcgctgcctt ctttgttgcc
     2521 aagagtctca cctcccttcg cagtgtcaag ttaggtccac tacctgatcg gctacacggc
     2581 ctgaatcagc atgtcgtcca aggcttcgaa cgtattggga ccgacttttc tgaagcgttt
     2641 atcgcgcttg agcgacgtcg caatcgacgt gagcggcgtt tttcctttga tctgcaaccc
     2701 gccttcgatt aaacgctgca ccagttcctt cgcgtgcatc gcccgattcg attcacgaag
     2761 gatttcatag gctgcttgag ggacgctttt ccccacgtat ttacttcgac cgagaagaat
     2821 ttcccgtgat tgatcggtga cgtccataac gggtacaggt cgaatccctt tagaatcatc
     2881 cgttgtaatg atctggttgg agagactggc aagcttggcc ttatcggcct cgacacgata
     2941 gagggtttca gcaagctcaa ggtatttttt aatagtggcg atttcgtcat caagtcggcg
     3001 acgcttcttc tctagttcct ggaaccggct cttgtacgcg ttgatccgtt gctctaaacc
     3061 aacgagaatg tctgttaatt cctccacaat ctcctcatat aaagcatgct tgctttatag
     3121 cattgcttgt acgttgtgtc aagacttatt attcgaatca tacgttgact attatgttta
     3181 gtttgttgca ggcaagtaat cctttcatag atcatgtcga taagccttgt accgacacaa
     3241 ggcccatgat ttcctcaagt ggacaattcg gtcatgctac aatcaggcgt gtccattcct
     3301 cacgaaccaa tctcgcgtga cgcgctcctc gaagcagcgg tcgccgccgg ccaagaagcc
     3361 ggaaccctgc tgcttgaata tgccggagcc ggttttcaca tcgagtataa gaacccgatc
     3421 aatctagtca ctgacgccga tcgtgctgcc gagcagtgtg tcatcaagcg tattagggct
     3481 cattttccaa cccatcggtt cttggccgaa gaacaaggtc gcattgaaaa ggcacagtcg
     3541 ccttatctgt ggatcatcga tccgctggat gggacgacta attttgcgca tggctatcca
     3601 acctactgcg tctccatcgg tctcgagtac gaggggcgct gtgtgcttgg cgtggtcttt
     3661 gatcccacgc ggaatgacct gttcacggcc attgaacatc ggggtgcgca gttgaacggt
     3721 cgccccatcc atgtgtccaa tacgatgggc ctcgacggca gcctattggt gacgggcttc
     3781 gcctatgaca tcagagaaac gccgcgaaat aacctcgatc atttttccaa ttttgccctc
     3841 aagacccagg gactcaggag aacgggatct gcggcattgg acctctgcta tgtggcggca
     3901 ggacgcttcg atgggttctg ggaagtccgg ctcaatccat gggatat
//
LOCUS       DATNDD010000206         3291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2241404, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000206 DATNDD010000000
VERSION     DATNDD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241404"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2985)
                     /gene="rpoC"
                     /locus_tag="VN647_04970"
     CDS             complement(<1..2985)
                     /gene="rpoC"
                     /locus_tag="VN647_04970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWG96418.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PTKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVCVDPGSTDLTEKELVPEDKLRTLVSEFGSSGFKV
                     GIGAEAIRDLLRKIDINALWDDLQVKAKASTSAAMKKKYAKRLKVLEAFRKSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEARGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGNQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERLGAKGEGKV
                     FGSSEEVRIAFDAREVEEHARIKVRLAGSLVQTTVGRVLLSEILPPGLPFANANKLMT
                     KKEMTKLIDAVYRQTGHRDTVEFLDKIKDIGFNYATRAGLSICIDNMHIPSKKEDFIG
                     KAQREVNEIEKQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGDPAKAESF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTH
                     GARKGLADTALKTANSGYLTRRLVDIAQDVIINEIDCGTRDGIIVSALVEGGEIIQPL
                     EERVLGRLAAEDIRDPVTGEIIVSWNEEINEELTKSIVEAGVDRVKIRSVLTCQSPRG
                     VCRACYGRDLARGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLE
                     AKHAGRIKFMSFDAKKNADIHNAGIAVRNKEGEWVVMNRNTKIAIVDDSGREREKYPV
                     VYGAKIKIKDGDPVVVGQKLVEW"
     gene            complement(3089..>3291)
                     /locus_tag="VN647_04975"
     CDS             complement(3089..>3291)
                     /locus_tag="VN647_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96419.1"
                     /translation="QAYGAASTLQEFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPES
                     FNVLVKELQSLGLDVELVKTQD"
BASE COUNT          693 a         1027 c          781 g          790 t
ORIGIN      
        1 ccactcgact aatttctggc cgacgaccac cggatcgccg tctttaatct tgatcttcgc
       61 cccatacacc acggggtatt tctcacgttc tcgtccgctg tcgtcgacaa tcgcaatctt
      121 cgtattgcga ttcatcacaa cccattcacc ctctttattc cgcaccgcaa taccggcatt
      181 gtggatatcg gcattctttt tggcatcgaa gctcatgaac ttaattcgtc cggcatgctt
      241 cgcctcaagc accgtttgct cgacgacctt actggctgtg ccgccgatgt gaaaggtccg
      301 catcgtcagc tgggtgcccg gctcgccgat ggattgcgcg gcgatgacac cgaccggttc
      361 gcctttttcc accagccgcc ctcgtgccag atcgcggccg taacaggcac gacagacacc
      421 acgcggcgat tgacaggtca gcacggaccg aatcttgaca cggtcgactc cggcctcaac
      481 gatcgacttc gtcaattctt cattgatttc ttcattccaa gacacaatga tttcccccgt
      541 gacggggtca cgaatatctt ccgccgccaa acgaccaagg acgcgttcct ccaacggctg
      601 aataatttca ccgccttcca ctaaggcact gacgataata ccgtcccgcg ttccacaatc
      661 gatctcgttg ataataacgt cttgggcgat atcgaccaat cgccgagtca gataccctga
      721 attggcggtt ttcagcgccg tgtcggcaag acccttacga gcaccgtggg ttgagatgaa
      781 gtactgcaac accgtcaacc cctcacggaa attggctgtg atgggcgttt cgatgatctc
      841 accggacggt ttggccatca gcccgcgcat accgcccaac tgccgaatct gctgcgagct
      901 gcctcgcgcg ccggaatcgg ccatcatgaa gatcggattg aacgattcgg ccttcgccgg
      961 atcacctccg gcgccgagtt ccttcatcat ctcgttggcc acctgctcag tgacatgcgc
     1021 ccaaatgtcg atcaccttgt tgtaccgttc gccgttcgtg atcaagcctt ctgaatactg
     1081 tttctcgatc tcgttcactt cgcgttgtgc tttcccgatg aagtcttcct tcttactcgg
     1141 gatatgcata ttatcgatgc agatcgacaa gccggctctg gtcgcatagt taaagccgat
     1201 gtctttgatc ttatccagaa actcgaccgt gtctcgatga ccagtctgtc gatacacagc
     1261 gtcaatgagt ttcgtcatct cctttttagt catgagcttg ttcgcattcg cgaagggcag
     1321 gcccggaggg aggatttcgg acagcaagac acgaccgacg gttgtctgca ccagcgaacc
     1381 ggcaaggcgc actttgatcc gcgcatgctc ctccacttct ctcgcgtcaa aggcgatcct
     1441 gacttcttcg gaagacccaa acactttccc ttcgcccttg gcgcctagcc gctctttcgt
     1501 caaccaataa cagcccaaca ccatatcttg cgacggcacc gcgatcggct tgccgttggc
     1561 cggggagaga atgttgttga tggacatcat gagaacccgc gcttcaacct gcgcttccac
     1621 agacagcggg acgtgcaccg ccatttgatt cccatcgaag tccgcgttga acgccgcaca
     1681 gacgagcggg tgcaatcgga tcgccttgcc ctccaccaac acaggatcga atgcctggat
     1741 gcccaaccga tggagcgtcg gggctcgatt cagcaatacc ggatgctctc ggatcacttc
     1801 atcaaggaca tcccaaacct caggccgttc tttttcgacc agccgtttgg cgctcttaat
     1861 ggtggtagcc gcccctctgg cttccagctt gtggaagatg aacggcttga acaattccag
     1921 cgccattttc ttcggcaacc cgcactgatg gaggcgtaac tccggcccga cgacgatcac
     1981 ggtacgaccg gaataatcga cgcgcttccc gagcaggttc tgccggaacc gtccctgctt
     2041 tcccttcagc atgtcgctca acgatttcaa cggacgtttg ttcgggccac gaatcgcgcg
     2101 cccgcggcga ccgttgtcga agagagcatc cacggcctcc tgcaacatcc gcatctcgtt
     2161 cctgatgatg acgccgggcg ccttcagttc catcaatcgt ttcaaccgat tatttcgatt
     2221 gatcacgcgg cggtagagat cgttcagatc ggacgtggca aaccgtcctc catcgagcgg
     2281 aaccaaagga cgcaattccg gcggcaggac cggaatgaca tccatgatca tccactccgg
     2341 tttattcccg gacttgcgga acgcctccag cactttcaac cgtttggcgt acttcttctt
     2401 catggccgcc gaggtcgacg cttttgcctt tacttgtagg tcatcccaca gcgcattgat
     2461 gtcgatcttc ctcagcaagt cgcggatggc ctctgcgccg attccaacct taaacccgct
     2521 cgaaccgaac tcggaaacaa gggtacgcag tttatcttcc ggcaccaact ctttttccgt
     2581 cagatcggtt gagccgggat ccacacacac ataactttca aaatagagga ttttttcgag
     2641 ctgcttgagg ctcatatcga gcagggttcc gatccgactc gggaccccct tgagaaacca
     2701 aatatgggcg acaggcgcag ccagctcgat atgccccatc cgttcgcgcc gaaccttcga
     2761 ttgaatcacc tcgacaccgc atttgtcgca cacgatcccg cggtgtttca tgcgcttgta
     2821 tttcccgcaa ttgcattccc agtccttggt aggaccgaag atcttggcac aaaacagccc
     2881 gtctttttcc ggtttgaatg accgataatt aatcgtttcc ggtttcttga cttcaccata
     2941 ggaccacgat cggattttct ccggtgatgc gatgcgaatc cgcatcgaat cgaacgacac
     3001 cgaatcccgt tgcttttcaa atagtgtata tacgccttcc aaggtagtga cctcctctga
     3061 tgccggtttt cagccggcac acaagcggtt agtcttgcgt cttgaccaac tctacgtcga
     3121 gccccaagct ctgtagttct ttgaccaaca cattgaacga ctccggcaac ccgggctcga
     3181 ggaacggctc acctttgaca atcgcttcat acatacgtga tcgacccggc acatcgtctg
     3241 atttgacggt aaggaactct tgcagagtcg atgccgcccc ataggcctgc a
//
LOCUS       DATNDD010000207         7067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1151822, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000207 DATNDD010000000
VERSION     DATNDD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151822"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VN647_04980"
     CDS             complement(<1..699)
                     /locus_tag="VN647_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HWG96420.1"
                     /translation="MKEGLRVSEEMEIQKTFVPDVDADDAKASGMLGMISREAEGPGS
                     EEKNQPVMRTSRGASPFLLEALYFRSFGERGLLTREEETSIAKQVDHGTRRIRTALRQ
                     AVRALFKSKRTSALTDSVKVLQMIRQLSGLSATALDKAEKALSTALNPSDPDMKPVQA
                     TAKTLKALLDEIRAARVILEQGKDELVRCNLRLVVDVAKHYTGRGLSLLDLVQEGNIG
                     LMKAAERYQYRKGFK"
     gene            complement(951..1697)
                     /locus_tag="VN647_04985"
     CDS             complement(951..1697)
                     /locus_tag="VN647_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWG96421.1"
                     /translation="MSLLMPLVLLGLVLYASFKRVMEYERLVVFVLGKFQTVKGPGIR
                     LVVPVLQQMVRVNLQTVTMEVPSQDVITRDNISVKVNAVIFLRVVDPQRAVLAVQDYL
                     YSTSQVAQTTLRSVLGQSQLDDLLSKRDDINAELQRIIDQQTEPWGVKVAAVEVKNVD
                     IPQDMQRAIARQAEAERERRAKIIHAEGEFQAAQKLAEAADVISRNPAALQLRYLQTL
                     VEIAAEKNSTTIFPIPVDTIAPFMKGLLSK"
     gene            complement(1722..3026)
                     /locus_tag="VN647_04990"
     CDS             complement(1722..3026)
                     /locus_tag="VN647_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HWG96422.1"
                     /translation="MRTMLVRVLTAVIVGPGLVLCLILPTDSVLAGDIIVANYEGVIN
                     PVAAEYLHDALAYAQISSAQALIFKLDTPGGLDTSMRLMIKDMTGSPIPVIVFVAPSG
                     GRAASAGVFITMAAHIAAMAPGTNIGAAHPVAMGGEMDNTMKEKVENDAVAYIKSIAE
                     QHGRNVSWAEDAVRKSISSTEQEALKLKVIDMIAEDVPVLLKHLQGRKIAVPHGSITF
                     SNEPPTLREFPMGTRLELLKILSDPNIAYLLMSIGTIGIMAELYSPGAILPGIIGAIS
                     LILAFYSLQSLPVNYAGAFLVILGAVFLLLEISVTSYGLLALGGLVAMTLGGLFLIKS
                     DAPFLQISLSFLLPTVMTIGGLLGIIAWTAVKSNRGRPVTGVEGMIGSIGIAKTDLNP
                     RGQITVQGEIWEAVSQTPIRQGEAAEVMSVEGLTVKVTPTHR"
     gene            3145..4029
                     /locus_tag="VN647_04995"
     CDS             3145..4029
                     /locus_tag="VN647_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HWG96423.1"
                     /translation="MRGMSRFFNGERIHLVDQKRRQYALTLKAGETYQFSGQKIAHDA
                     LIGRLDGSIVTLSGGKKMVALRPTFGDYVLKMPRGAQVLYPKDLAIIPMWADIYPGAR
                     VFEAGTGSGALTMALLRAVGPGGLVVTYEIREDFARTAKTNIARSLNPINLICFRKNA
                     YEGIDLLDDQVPFDRVVLDLPEPWQVVPHAMKALRSGGIYLSFVPTVPQVMQTVEALE
                     RTAVFGMIETFEALLRTWSVQGRSVRPDHRMVAHSGFITVARKVEPGLLGPMPDIGAP
                     ADRDQEGTQDEAQEEPNL"
     gene            4026..4787
                     /locus_tag="VN647_05000"
     CDS             4026..4787
                     /locus_tag="VN647_05000"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG96424.1"
                     /translation="MNRLEGKVVVVTGGNAGIGEAIAKRFVEEGASVVITGRRQQELD
                     RVTSVIRLNKGKVLGIAGSVTDETHVQDVARRTLDSFGRIDVLVNNAGIGEFGKRLHE
                     TEDAIWAQVFDINVTGVFRMTRAVIPHMLRQGRGSIINISSIASLVGLSGSAAYAASK
                     GALDALTRALAVEYAKEGIRCNVVNPGLIDTPMAAPLMANPDMLQPILAQYAIRRPGT
                     PEEVANMVLYLASEEAAWVTGATFPIDGGMTVCKG"
     gene            4830..5684
                     /locus_tag="VN647_05005"
     CDS             4830..5684
                     /locus_tag="VN647_05005"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWG96425.1"
                     /translation="MDIDVRTQFCGVIGNPVGHSLSPAIHNAAFRKLGLNFVYLAWQV
                     QTIGDAVKGLRALGNFRGASVTIPHKVAAIPFLDHVERTAQQIGAINTIVAEQGALTG
                     YNTDATGALRALREGGAELKGRRIVVLGSGGAARAIAFALAVESSADKLTLLGVDDSE
                     RASLAQDIRTQASLTVEDFHLDETTLRRVLPDAHVLIHCTPVGMSPNADTTCIPASLL
                     HNDLSVMDIVYNPLETRLLKDAKRAGCRAIPGLEMFLNQAVTQFEFWTNHPAPVDVMR
                     SVLESHFR"
     gene            5681..6181
                     /locus_tag="VN647_05010"
     CDS             5681..6181
                     /locus_tag="VN647_05010"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWG96426.1"
                     /translation="MNVVLIGYRGTGKSTVGKILAARLDHILVSTDAEIVKLVGQSIP
                     EIVEQNGWEHFRDLEAKVCQALAGRTGLVVDTGGGAILRSRNVEVLKATGRLFWLTAS
                     VGTIAERIGRDSQRPSLTGVKSFVDEIQDVLRERTPKYHAAADYVVETDERSVTQVAE
                     EILARL"
     gene            6245..6321
                     /locus_tag="VN647_05015"
     tRNA            6245..6321
                     /locus_tag="VN647_05015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6279..6281,aa:Arg,seq:tcg)
     gene            6763..>7067
                     /locus_tag="VN647_05020"
     CDS             6763..>7067
                     /locus_tag="VN647_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96427.1"
                     /translation="MKPVFLNVVLLVAAGATLMSGCTSTTAKTEFELSSSDSPQDHRK
                     IATYYRHEAARLRQVSEDLSVRINVYERLFGPTSDWVAGTRLLAQSYEDAAQTYERKA
                     "
BASE COUNT         1572 a         1877 c         2008 g         1610 t
ORIGIN      
        1 tttgaatccc tttcgatact gatatcgctc cgccgctttc atcagaccga tgttgccttc
       61 ttgtacgaga tcgagcaagc tcaatcctcg ccccgtgtag tgtttggcaa catccaccac
      121 caaacgaaga ttacaccgca cgagctcgtc tttgccttgc tccaagataa ccctcgcggc
      181 gcggatttca tcgagcaacg ctttcagcgt ttttgctgtg gcttgcacgg gtttcatgtc
      241 tgggtccgac ggattaaggg cggtgcttaa ggccttctcg gccttatcca atgccgtagc
      301 ggaaagtcca ctcaattgcc tgatcatttg gagtacctta acgctgtctg taagagcgga
      361 tgtccgcttg gatttgaaca aggctcttac ggcttgacgc agagcagtcc gaatgcgcct
      421 ggtcccatgg tccacctgct tggcgatcga agtttcttct tctcgagtta aaagtccccg
      481 ttcgccgaac gaccggaagt acaaggcctc caaaagaaac gggctggccc cgcggcttgt
      541 ccgcataacc ggctgattct tctcctccga tccgggtccc tcagcctcac ggctgatcat
      601 tcccagcatg ccactagcct tggcgtcatc ggcatcgaca tccgggacga aggttttctg
      661 tatctccatc tcctcagaga ctcgcagtcc ttctttcatc gtgtcacctt ttgctctggt
      721 tgaaggtcgt ccactcctat tggagccata ttcatgggaa aagattcaac tgagtactgc
      781 ttctacgaag caaaagggat gctggagtga agatgagaaa cggtgcggag ggatcagcac
      841 gaaaaatcag aaagattaga ttgggctaat gaggaaacga agggaagaac tgtcacactt
      901 atgcggcatc tatgtaagat cggcatcagc gattgggctg aaatgccaca ctacttcgat
      961 aacagtcctt tcatgaatgg ggcaatggtg tcgactggaa tcgggaagat ggtcgttgag
     1021 ttcttctcag ccgcaatctc gacgagtgtt tggaggtagc gcagttgaag ggcggctgga
     1081 ttccggctga tgacatcggc ggcttcagcg agtttctgcg ccgcttgaaa ttcgccttca
     1141 gcatgaatga ttttggcccg ccgttcccgc tcggcctcag cttgtcgggc gatcgcgcgt
     1201 tgcatgtctt gagggatatc cacgttcttt acttcgacgg cggcaacttt gaccccccat
     1261 ggctcagttt gctgatcgat aatccgctgt agttcagcat tgatgtcatc gcgcttcgat
     1321 aacaggtcgt cgagttgact ctggccaagc acgctgcgca gcgtggtttg ggcaacttgc
     1381 gacgtggaat acagataatc ttggactgca agcacggccc gttgagggtc aaccaccctg
     1441 aggaaaatga cggcgttcac tttcacggaa atattgtccc gcgtaatgac atcttgggag
     1501 gggacttcca tcgtgacagt ttgaagattg acgcgcacca tctgttggag aaccgggacc
     1561 accaaccgaa tgcccggtcc tttcacggtc tgaaacttgc ctagcacaaa caccacgagc
     1621 cgttcgtact ccatgacgcg cttaaaacta gcgtagagca cgaggcccag gaggactaac
     1681 ggcatgagca atgacataaa ggctccttat cttcgatcgg tttacctgtg agtgggggtc
     1741 accttcacgg tcagtccctc gaccgacatc acctctgctg cctctccctg ccgaatcggg
     1801 gtttggctga cagcctccca gatctcccct tgaacggtaa tctgaccgcg tgggttgaga
     1861 tccgtcttgg cgatcccgat cgaaccaatc atcccttcca ccccggtgac cggtctgccg
     1921 cgattgctct taacggccgt ccacgcaatg attccgagca ggccgccgat cgtcatgacg
     1981 gtcggcaata aaaaggatag ggatatttgc agaaaaggcg cgtcgctctt gatgaggaat
     2041 agaccgccca gcgtcatcgc aaccaatcca ccgagggcaa gcaacccata gctggtgacc
     2101 gaaatttcga gcaggaggaa aaccgcaccg agtatcacaa gaaatgcgcc tgcatagttc
     2161 acggggagcg attgaaggga atagaacgcc aggatcaagc tgatggcgcc gatgataccg
     2221 ggcaggatgg cgccggggct gtagagttcg gccataattc caatggttcc gatggacatc
     2281 aggagatagg cgatgttggg atcactcaag attttgagta gttccaggcg agttcccatc
     2341 gggaattcac ggagagtcgg gggctcattt gagaacgtga ttgacccatg aggaacggca
     2401 atctttctcc cttgcaggtg tttcagcaac acagggacgt cttccgcaat catatcgatg
     2461 actttgagtt tcaaagcttc ctgctctgtc gaggagatgc tctttcgtac tgcgtcttcg
     2521 gcccacgaca cattgcgccc gtgctgttca gcgatgcttt tgatgtaagc aaccgcgtca
     2581 ttctccactt tctctttcat cgtgttatcc atctcaccgc ccatcgcgac tgggtgtgct
     2641 gctcctatgt tagtacccgg cgccatggcg gcaatatggg ccgccatcgt gatgaagact
     2701 cctgccgagg cggctcggcc tcccgaaggg gcgacgaaca cgatcaccgg gatgggcgag
     2761 ccggtcatat ctttgatcat cagccgcatg gaggtatcca atcctccagg ggtatccagc
     2821 ttgaagataa gagcctgcgc gctcgatatc tgagcatacg caagagcgtc atgaagatat
     2881 tcagctgcaa cagggttgat gaccccttca tagttggcaa cgatgatgtc tccggcgaga
     2941 acggaatcgg ttgggagaat caagcagagt actaaccccg gtcctacaat caccgctgta
     3001 aggactcgga cgagcatcgt tctcatctcc cgaccatcac aaaggcatta tgaaaattat
     3061 caatgatgag aagatcttac ggtcccccta ctaccctgtc aaggaagggc gcttggttgc
     3121 aaaccatgga acgaacctcc tagaatgcgc ggcatgtctc gctttttcaa tggcgaacgc
     3181 atccaccttg tcgatcaaaa acggcgacag tatgctctca cattgaaagc gggggagacg
     3241 tatcagttca gtgggcagaa aatcgcacat gatgcgttga tcggccgtct cgacgggtcc
     3301 atcgtcacac tgtccggcgg taagaagatg gtggcgctcc ggccgacctt cggcgactat
     3361 gtgctcaaga tgccccgagg ggcgcaggtt ctatatccta aggatctcgc gatcataccc
     3421 atgtgggccg atatctaccc gggtgctcga gtcttcgagg ccgggaccgg ttcaggagcc
     3481 ttgaccatgg ctctattgcg cgcagtgggg ccgggtggat tggtggtgac gtatgagatc
     3541 cgggaagatt ttgcacggac ggcaaagaca aacattgccc gttctctgaa tccaatcaat
     3601 ctgatctgtt tcaggaaaaa tgcctatgag ggcatcgatt tactggacga ccaggtgcct
     3661 ttcgatcgcg tggtgcttga tttgccggaa ccatggcagg tcgtaccgca tgccatgaaa
     3721 gcccttcgat caggcgggat ctatctgagc ttcgttccca cagtaccgca agtcatgcaa
     3781 acggtagagg cgcttgagcg cacagctgta tttggaatga tcgaaacgtt tgaggcgttg
     3841 ctgaggacct ggtccgtgca gggcaggagc gtgcggcctg atcatcgcat ggtggctcat
     3901 tctgggttca tcacagtggc tcgtaaggtt gagcccgggc tccttgggcc aatgcctgac
     3961 ataggagctc ctgccgatag ggaccaggaa ggcacacagg atgaagcaca agaggaaccg
     4021 aacttatgaa taggttggaa ggcaaggtag ttgtggtgac gggaggcaat gccggcatcg
     4081 gtgaagcgat cgcgaaacgt ttcgtcgagg agggagcatc ggttgtcatc acggggcgac
     4141 ggcaacagga attggatcgt gtaaccagtg taatccgact caacaaagga aaggtactcg
     4201 gaattgccgg ttcagtgacg gatgaaactc atgtgcagga tgtcgcccgt cgaacccttg
     4261 atagttttgg gcggatcgac gttctggtca acaatgcggg aatcggtgaa tttggaaaac
     4321 ggctccacga aacggaggat gcgatctggg cgcaagtgtt cgacatcaac gtgaccgggg
     4381 tattccgcat gacacgggcc gtcatccccc acatgctgag gcaaggccga ggctcaatca
     4441 ttaatatctc gtcaatcgcc agtttagtag gcctctccgg ctcagccgcc tatgcggcat
     4501 ccaagggtgc gcttgacgcc ttgacgcgtg cgctggcggt cgaatatgcg aaggaaggaa
     4561 ttcgctgcaa tgtggtgaat ccgggcctga tcgatacgcc tatggcggct cccctgatgg
     4621 ccaatcccga catgctccag cccattcttg cacaatatgc gattcgtcgt cctgggacgc
     4681 cggaggaagt ggcgaatatg gttctgtatc tggcctcaga ggaagccgcc tgggttacgg
     4741 gggcgacctt ccccattgac ggaggcatga ctgtttgtaa agggtaatgg ttgctgccca
     4801 cgaacagtac aacttccgtt aggtgaagaa tggacattga tgttcgaaca cagttttgcg
     4861 gggtgatcgg caatccggtt ggccattcac tgtcacctgc cattcacaat gcggcgtttc
     4921 gcaaattggg gctcaacttt gtctatcttg cctggcaagt ccagacgatc ggcgatgccg
     4981 tcaaagggct tcgcgcactc ggaaattttc gtggggcaag cgtgacgatc ccgcacaagg
     5041 tggccgccat accgtttctt gatcatgtgg aaaggacggc tcaacaaatc ggtgcgatca
     5101 atacgatcgt tgctgaacag ggtgcgctca ctgggtacaa cacagatgcg acaggcgctc
     5161 tgagagcgtt gagagagggt ggagctgagc tcaaaggccg acgcatcgtc gtcctcggtt
     5221 ccggaggcgc cgctcgagcg atcgccttcg ccctggcagt tgaatccagt gcggacaagt
     5281 tgacgttact gggagttgac gattctgaac gggccagttt agctcaggat attcgtaccc
     5341 aggcatcatt gacggttgaa gactttcatc ttgatgaaac cactcttcga cgggttctcc
     5401 ccgacgcaca cgtgctaatt cattgtaccc cggtcggtat gtctccgaac gctgatacca
     5461 cctgtattcc tgcttcgctc cttcacaacg acctctccgt catggatatc gtctacaacc
     5521 cactcgaaac cagattgctc aaagatgcga agcgtgcagg gtgtagggcg attcccgggc
     5581 tggagatgtt tctcaatcaa gccgtcactc aatttgagtt ctggacgaat catcctgcgc
     5641 cggttgatgt gatgcgttcg gtcttggaat cccacttccg atgaatgtgg tgctgatcgg
     5701 ctatcgtggg accggcaaga gtaccgtcgg caaaatactg gcggctcggc ttgatcacat
     5761 tctcgtgtcg actgatgcgg agatcgtgaa gctagtcggt caaagcattc cggagatcgt
     5821 cgagcaaaat gggtgggagc atttccgtga tctcgaagcc aaggtctgcc aagcactggc
     5881 cggtcgcacc gggttagtcg tcgatacggg cgggggagcc atccttcgat cacgaaatgt
     5941 cgaggtatta aaggcaacag gaaggttgtt ctggctgacc gcctcggtcg ggacgatagc
     6001 cgaacgtatc ggtcgggatt cacaacgccc ttcccttaca ggagtcaagt cattcgtgga
     6061 tgagatccaa gatgtccttc gtgaacgcac accgaagtat cacgcggctg ccgattacgt
     6121 cgttgagact gatgaaaggt ccgtcacgca agttgccgaa gagattctgg cgcgattgta
     6181 gcatctcaac cgcctctgtc agccttgaca agcctcgcct gaacatgatt catgtaggtc
     6241 cgctgcgccc gtagctcagt tggatagagc gccgggcttc gaacccgtag gtcgcaggtt
     6301 caattcctgc cgggcgcacc atagattcaa cgagttagca gaatcctccc cgctgatgtg
     6361 tcctcctggt tgcgcggggg ttgcagatcc aaccccatcc cagcgcggcg tgaccggttc
     6421 atcgagccga tccacggcct gccgattccc tcccgggatt aaatgcccga tcattgctca
     6481 ggatctcaag caataccgat tggaccgtca cgatgttcaa attctgtgac gcttgattat
     6541 gtcctacggt catacctccg tgaaaaacca ggccatcatg aagcatgacg cggaacaaga
     6601 gggggggcgc cagcacattc gccaggcgta ggcgtaggag aaggccgaga ccatgctagg
     6661 cgctcggatt ggcattcgga tcttcccggg cgttatgatt gttggagctg ggtgttgtcg
     6721 agcatgagcg cgcgtcatgg ggattcaagt gagacttagg gggtgaagcc cgtgttcctc
     6781 aatgtcgtgc tgttggtggc ggcgggagcc accctcatga gtggctgtac gagcaccaca
     6841 gcgaaaaccg aattcgagct ctcgagctct gactctcccc aagatcaccg gaagatcgcg
     6901 acctactacc gtcatgaggc ggccagactg cgacaggtgt ctgaggactt gtcggttcgc
     6961 atcaatgtct acgaacggct gttcggtccg acctcggact gggtggcggg gacacgcctc
     7021 ttggctcaat cctatgaaga tgccgcgcag acgtatgaac ggaaggc
//
LOCUS       DATNDD010000208         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_68027, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000208 DATNDD010000000
VERSION     DATNDD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68027"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1499
                     /locus_tag="VN647_05025"
     CDS             246..1499
                     /locus_tag="VN647_05025"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWG96428.1"
                     /translation="MKPPTTDTLLEAPTPLAADELRLMDAYWRACNYLSLGMLYLCEN
                     PLLRESLRVEHIKKRLIGHWGSTPGQNFIWVHLNRVITRYDMNMIYISGPGHGAPAVL
                     ANAYLEGTYSEVYPDKSQDVEGLRRFFKQFSFPGGIGSHATPETPGSIHEGGELGYSV
                     SHAFGAAFDNPDLLVAAVIGDGEAETGPLMASLHSNKFLNPIRDGAVLPILHLNGYKI
                     ANPTISARISSDELHGMFAGFGWTPHLVEGDDPPVMHRKMATTVETCINQIRTVQEEA
                     RHRGKAVRPRWPMIILRTPKGWTAPKELDGHKLEGYWRAHQIPILDVAENPAHLAMLE
                     AWMRSYRPEDLFDETGSLVPQLRALAPTGHRRMSANPHANGGLLRIPLRIPDFRAYGV
                     SVIKPGAIEAQSTFLLGSISAGCHA"
     gene            1492..2646
                     /locus_tag="VN647_05030"
     CDS             1492..2646
                     /locus_tag="VN647_05030"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWG96429.1"
                     /translation="MRDNMHNFRVFGPDETASNHLQAIYETTKKTWLAEFKPEDSDGG
                     ELSQDGHVMEMLSEHTLEGWAEGYLLTGRHMFFASYEAFLHIIDSMVNQHAKWLEKCK
                     TEVKWRASIPSLNLLITSTVWRQDHNGFTHQDPGFLDVVTNKSPQVTRIYLPPDANCL
                     LSTADHCLRSVDYVNVIVADKQLHLQYLDMAAAVRHCEKGIGIWPWASTDQGSAPDVV
                     MACAGDVVTMEALAATAILRERFPDLKIRFINVVDLFKLEPITDHPHGLTNRDFDGLF
                     TTDKPVIFNFHGYPALIHKLAYRANHDLWHVHGYNEKGSIDTPLALAILNEVDRFNLT
                     IDVIDRVPRLQESGAHVKNWLQDQILEHLAYAHEQSIDPPEIRNWKWPYT"
BASE COUNT          616 a          783 c          798 g          551 t
ORIGIN      
        1 acccatgtcg ggatgccggc agacgtcatg gcacggcaag gactcacgag cgggctcgtg
       61 agaatctcgg tcggctgcga ggatatagaa gatgtgatcg gcgatctgga ggaggcgctg
      121 aaaacggact aagcgcctgc acgaacaatg cacgagtcga aggggtgggt ggcatgagtc
      181 cgcctcggag tctggagcga ccctgtcgtg cgataccgta gccatggatg aggagggact
      241 tcatcatgaa accgccaacg accgacaccc tgctcgaggc ccctacgccg ctcgccgccg
      301 atgagctgcg tctgatggat gcctactggc gcgcgtgtaa ttatctttcg ctcggcatgc
      361 tctatctgtg cgagaaccct ctgctgcgag aatcgcttag ggtggagcac atcaaaaaac
      421 gattgatagg acattggggc tccactcccg gtcagaattt tatctgggtc cacctgaacc
      481 gggtgatcac acgctacgac atgaacatga tctacatctc cggacccggg cacggtgcgc
      541 ctgcggtgct cgcaaacgct tatctggaag gcacctattc cgaggtgtat ccggataaga
      601 gtcaagatgt agaaggcctg cgcaggttct tcaagcagtt ttcgtttcca ggagggatcg
      661 gcagccatgc cactccggaa acgcccggtt ccatccatga gggcggcgaa ttggggtata
      721 gcgtgtcgca tgcgttcggg gcggcgtttg ataacccgga tttgctcgtc gccgccgtca
      781 tcggagacgg tgaagccgaa accggtccac tgatggcgtc tctgcattcc aacaaatttc
      841 tgaatcccat tcgcgacggc gccgtgctgc cgattcttca tctgaatggg tacaagatcg
      901 ccaatccgac catttcggct cgcatctcat cagacgaatt gcacggcatg ttcgccggct
      961 ttgggtggac tccccacctc gtcgaggggg acgatccgcc ggtcatgcac cgtaagatgg
     1021 cgacgactgt cgagacctgc atcaatcaaa tccgcaccgt tcaggaggag gcgcgccatc
     1081 gcggcaaagc cgtacgaccg cgctggccca tgatcatcct ccgcacgccc aaagggtgga
     1141 ccgctcccaa ggagttggac gggcacaagt tggaaggtta ttggcgtgcg caccagattc
     1201 cgatcctcga tgtcgccgag aatccggcgc acctcgcgat gctcgaagcc tggatgcgga
     1261 gttacagacc ggaagatctc ttcgatgaaa ccggctcact ggttccgcaa ctgagggcat
     1321 tggctccgac cggacaccga cggatgagtg ccaatcccca cgcgaacggc ggcctgctgc
     1381 ggattccgct tcgaattcct gattttcggg catacggcgt gtcggttatc aagccggggg
     1441 ccattgaggc ccaatcaacg tttcttttgg ggtcaatttc tgcgggatgt catgcgtgac
     1501 aacatgcata acttccgggt cttcggaccg gacgaaacgg cgtccaacca tctgcaagcc
     1561 atttacgaga ccaccaagaa gacctggctg gcagaattta agccagagga ctcggatggc
     1621 ggcgagctgt cgcaggatgg acacgtcatg gaaatgctca gcgaacatac ccttgaggga
     1681 tgggcggagg gctacctatt gaccggccgc cacatgttct tcgcctcgta tgaagccttc
     1741 cttcacatca ttgattccat ggtcaaccag catgccaagt ggctggaaaa atgtaaaacc
     1801 gaggtcaagt ggcgggcatc tatcccgtcc ttgaatctgt tgatcacctc gaccgtctgg
     1861 cgtcaggacc acaacggctt cacccatcaa gacccggggt ttcttgatgt ggtgaccaac
     1921 aaaagtccgc aggtcacccg catctatctg ccgcccgacg cgaattgttt gttgagcaca
     1981 gcggatcact gtttgcgtag cgtcgattat gtgaatgtga tcgtggcgga caagcagctg
     2041 caccttcaat acttggacat ggctgccgcc gtgaggcatt gcgagaaggg catcgggatt
     2101 tggccctggg ccagcacaga tcagggcagc gcgccggacg tggtgatggc ctgcgcgggc
     2161 gatgtcgtca ccatggaggc gctggccgcg acagccatct tgcgagaacg attcccggat
     2221 ctgaagattc ggttcatcaa cgtggtcgat ctgttcaaac tcgagccgat cacggaccac
     2281 cctcatgggc tgacgaatcg cgacttcgac ggcctgttta cgacggacaa acccgtcatt
     2341 ttcaacttcc acggataccc ggcgctgatt cacaagctgg cctaccgggc caaccacgac
     2401 ctctggcatg tccacggcta caatgaaaag ggaagcatcg acacgccgct cgcgctggcc
     2461 atactcaatg aggtggaccg gttcaatttg acgatcgacg tcatcgatcg agtgccacgg
     2521 ctacaagagt cgggagctca cgtcaagaat tggctgcaag accagatcct ggagcatctc
     2581 gcatatgccc acgaacaaag catcgatccg ccggagatcc gaaactggaa atggccgtac
     2641 acgtaagaaa gcgccgcgat tgcacagatt tttttcatac cgaaaggagt ggactcatgg
     2701 ctgtcacaac accggaacga acgacgttgg acatcctgca ggacaagg
//
LOCUS       DATNDD010000209         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_81757, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000209 DATNDD010000000
VERSION     DATNDD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81757"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..612
                     /locus_tag="VN647_05035"
     CDS             265..612
                     /locus_tag="VN647_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWG96430.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAETKGNHVGDGQGI
                     GDNIYVIAHDRCTECVGHFDEPQCAAVCPVDNCCISDPLYPETTDVLLERAKTLNPDK
                     AIDAAKVWSGVRN"
     gene            complement(748..1224)
                     /locus_tag="VN647_05040"
     CDS             complement(748..1224)
                     /locus_tag="VN647_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96431.1"
                     /translation="MGKTLLAVFLGLGMVLAGASGSYALQAGGVETDDLETGSVVGQP
                     FKELTVDASFYSIDIGNMKTWYPPTTVIDFKGRPGAPVLIKVTNNSAAEHGFQLSAAV
                     NQSGPFVLDTKLVLKPGETRYIGVPTSDLFYAAGNVLEYRCHLHPAHVGGKLVMLK"
     gene            1593..>2005
                     /locus_tag="VN647_05045"
     CDS             1593..>2005
                     /locus_tag="VN647_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG96432.1"
                     /translation="MPIILAVNGIVETYPIKPVGPRNGRAESVGEREHRGQHSPSLDR
                     SALAAQTAYQQQSNDRPYPKPAVLARDLMRAPVFSLPSDSTLLDAWASMSHKGFHHIP
                     VTTMHDTLVGMVSYRDLLHHVPELITANDKRQASGR"
BASE COUNT          497 a          536 c          519 g          453 t
ORIGIN      
        1 gttctctatt actcgcctga gcttgatcag atgccatgct gatgacgacc ggcgctttct
       61 tctctgttgg gcccaatggc ccatacacaa taatgggcta ggtcttctga ccggattccg
      121 ttcagttgac ttagaaaaga atcacggaat agaatgcatt tccgcgttgt tactcgccat
      181 aagccaccga ggcactatgg tgtgaggcgc gtggacttgt caatgttctt atagtctttt
      241 gacgtgataa aggaggctta ggtcatggct ttattgatta cggatgaatg catctcttgt
      301 ggagcatgcc tgccggaatg tccgaatgaa gcgatctttg aaaccagaag cgatgctgag
      361 accaaaggca atcacgtcgg cgatgggcag ggcatcggcg ataatattta tgtaatcgcg
      421 cacgaccgct gcaccgagtg tgtcggccac tttgacgaac ctcagtgtgc tgcggtttgc
      481 cccgttgata attgttgtat ttcagaccct ctctatcctg aaacgacgga tgtgcttctg
      541 gaaagggcga agactttgaa tcccgataaa gcgatcgatg ccgccaaagt atggagtggg
      601 gtaagaaact gattctctag tgcacttatt taagggccct ggttctgtcc agaaccaggg
      661 ccctttgttt tggaccgttg tggcgaaaaa tgaaaaggcc tcggtgagct tccaccgagg
      721 cctttttcac agtccgaacg aaatacgcta tttcagcata accaacttgc cgccgacatg
      781 ggctgggtgc aagtgacaac gatattcaag gacgtttccc gctgcataaa acaaatcgct
      841 ggttggaaca ccgatgtaac gcgtttcccc cggcttgagc actaatttgg tatccaagac
      901 aaacggtccc gattgattga ccgcagcact cagctgaaag ccatgttcag cagcggagtt
      961 attcgtcact tttatcagca ccggcgcacc gggacgcccc ttaaaatcga tgacggtcgt
     1021 gggaggatac cacgtcttca tgttgccgat atcgatcgag tagaacgacg catccacggt
     1081 cagctcctta aatggctgtc caaccactga accggtttcc agatcatcgg tctcaacacc
     1141 accggcttga agcgcatacg aaccggaagc cccagccaac accattccaa ggccgaggaa
     1201 gaccgccaac aatgtcttgc ccatgactac ccccctttgc aagataaaga agagataaat
     1261 agaggcttgt ggaatatatg atgtcaaact tggaggacgt gcaggctttt ggtaaagtac
     1321 gcctttcgtc tattgcccca gcaccgcttg tcaacactca ccacgtcaca cgacgatcct
     1381 tatagcatag ggctcaaaac ggaagcaacc gattggaacg cctaggtagt agcagtactc
     1441 aggcagttga gcggacaaga cagagaagga gcgccgccga gatcgagata gtgaggcagt
     1501 gaagccccgc gaaacggcgt tggagagctt ttgcaacatc ctcagcttaa gtatttcccg
     1561 aacattccga tacggcagtt gaggagctgc gcatgcccat cattttggcg gtcaatggaa
     1621 tcgtggagac gtatccgatc aagccggttg gcccacgaaa tggccgcgcc gaatccgttg
     1681 gagagcgaga gcatcgtgga caacactcac cttcattgga tcgctccgct ttggcggcac
     1741 aaacggctta tcaacagcag agcaacgacc gcccataccc gaaacctgcg gtcctggcgc
     1801 gggacctcat gcgtgctccg gttttctctc ttccatccga cagcaccttg ctggacgcct
     1861 gggcgagcat gtcacacaaa ggattccacc atatccccgt cacgacgatg catgacaccc
     1921 tcgtcgggat ggtgtcgtat cgtgacctcc tccaccatgt tccggaactg atcacggcga
     1981 acgacaagcg acaggcctca gggcg
//
LOCUS       DATNDD010000210         9684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_609920, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000210 DATNDD010000000
VERSION     DATNDD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609920"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VN647_05050"
     CDS             complement(<1..903)
                     /locus_tag="VN647_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96433.1"
                     /translation="MSDRNIKRIVIGLASFIFVTIYSVMIMPGISDVRHLSSCLIASY
                     LVLWGGYSLISTVPREEIRNQFVLMTIAFGAALLLVELPALVKLIDYRQTFSISGRIG
                     GGPGYLPDPELLFKPAPYHAVKMEFSRGNIGEILCLPSHPAEPFEVKYDKNGFRNDED
                     FTSADIAVIGDSYVESPMLPGSMLATTRLAKLTQKTVANLGQSGYGPQQELAVLKRYA
                     LPLHPKSVVWIFYEGNDLRDAQKYNDKVSFLKSNWNAIDSLWDRSFARNLLSWLTGMI
                     RGCTPVHAAKDVITHAVVMGPDQRK"
     gene            complement(1335..1772)
                     /locus_tag="VN647_05055"
     CDS             complement(1335..1772)
                     /locus_tag="VN647_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96434.1"
                     /translation="MNIGTKQVMHEPSVCRMGNLNTEAAVVAVTKDLKVSFPSRRREE
                     RVNEQKVCAYSLCESPDGKQVTIEQGEVYCINRSEHGILVLMGGQPRKRQLLELHVAE
                     TRWEYSLSLYEVQWTKKVPVESHGELFLVGCRLVFGASRYRAF"
     gene            complement(2177..3304)
                     /gene="dinB"
                     /locus_tag="VN647_05060"
     CDS             complement(2177..3304)
                     /gene="dinB"
                     /locus_tag="VN647_05060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWG96435.1"
                     /translation="MHRVIAHLDMDAFFAAIEERDTPAFRGVPLVVGADPLGGRGRGV
                     ASTSNYLARAYGIHSATPISTAWRLSEAARQTGKPPVTFVSVDMQKYARVSEEVMRIV
                     RRFIPCVEQASVDEVYGDVSWTGSYDEAERVCRRLKAEIHSEEQLTASIGIGPNKLIA
                     KIASGWHKPDGLIVVRAEEAEAFLAPLSIRVIPGIGPKTEGLLKAKNIRIVEDLRVLT
                     LHQLEALLGKRGVDLYEKVRARDDSPVEEYSELKSIGEQETFESDTLDSQTILNQLNG
                     LVRGVIDRLHHEGFHSFRTVVLTVRFADFVTKSRAHTLAMATDESAILRREAMKLMLP
                     FLDRRENPHRKLIRLLGLRVEKLNCEMNRVGLFDRLFTLPD"
     gene            3499..3684
                     /locus_tag="VN647_05065"
     CDS             3499..3684
                     /locus_tag="VN647_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96436.1"
                     /translation="MGYRWYNLIGFLMLLSALFLNEVVYLNVWYDLNFSVIVPALVYL
                     GVGLWLSLWGGPRRQNA"
     gene            3829..4017
                     /locus_tag="VN647_05070"
     CDS             3829..4017
                     /locus_tag="VN647_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96437.1"
                     /translation="MGMTFINWIGFVMLLLAFLLNEAFLGINAWYELNIMAVLAGVVW
                     LVVAAALSIWPSHRDQRA"
     gene            complement(4128..4703)
                     /locus_tag="VN647_05075"
     CDS             complement(4128..4703)
                     /locus_tag="VN647_05075"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWG96438.1"
                     /translation="MSDTKIRCGWVGLKPHLMRYHDEEWGVPVHDDRKHFEMLLLEGA
                     QAGLTWETILLRREGYRKVFVGFDPAVIARFTRRMEAALLQDVGIIRNRLKIEAAVTN
                     AQAFLTVQKECGSFDRYVWRFVGGKPRINRWKTLSQVPAIATESDALSKDLKKRGFRF
                     VGSTIVYAYMQAVGLVNDHTSDCFLGGDGIQ"
     gene            4954..5160
                     /locus_tag="VN647_05080"
     CDS             4954..5160
                     /locus_tag="VN647_05080"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HWG96439.1"
                     /translation="MAAKQYKQLGCLDVEPSAGCAFQVRTETEAELMQLVATHAKQCH
                     KLDSIPQEMAAKVKSAIKTVTVTV"
     gene            5255..5689
                     /locus_tag="VN647_05085"
     CDS             5255..5689
                     /locus_tag="VN647_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96440.1"
                     /translation="MGWKSVFTIAAMLLLSTSGCSYLFYPHAKEFTEKAKGATGVDTL
                     INLTNMAEATAKAAKGGKGADQPFDDLHNQFHAIDNSICCVEKSVKEQPAYALAVTHN
                     KELKTIFKRLWKFKNDQPQRDQHLDLFVSELQEMRQTLETLR"
     gene            5694..6551
                     /locus_tag="VN647_05090"
     CDS             5694..6551
                     /locus_tag="VN647_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWG96441.1"
                     /translation="MAGPSPEQVIDSQRQDWNRVAGGWEKWDRFFDEQMAFLNHRLVG
                     DSRLHSGLRVLDLGSGTGYPALLAAQAVGANGSVVGIDLAEHMLTIAERKATRLGLQN
                     VMFRVGDVTTLPFESASFDAVTSRFCLMFLPEIPKAAAEIARVLKPGSWLAAAVWSAP
                     EKNPSIGLSMTAVKQVIDLPPTDPLAPGIFRLAQPGDFAGMLKRAGLIDITDQEFLGE
                     WSYGSTDEYYTSLMEIAAPVQNLMTTLSEPQRQEVRRLIIKAASQFQRGARITFPIAV
                     RMVAGRKPG"
     gene            6555..6866
                     /locus_tag="VN647_05095"
     CDS             6555..6866
                     /locus_tag="VN647_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWG96442.1"
                     /translation="MPTKNDGFKNFILDQLTELKGVTARAMFGGYGLYQRATLFGIIH
                     KGRLYFKVSTTTVHNYMDHGMKPFRPNPKQTLKSLYGVPIDVIEDAEALTQWATEAVK
                     R"
     gene            6874..7416
                     /locus_tag="VN647_05100"
     CDS             6874..7416
                     /locus_tag="VN647_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWG96443.1"
                     /translation="MSHILWTIGHSTRPIDEFVSLLKAHGIQQLVDVRTVPRSRHNPQ
                     YNNEALLKSLRDEDMSYKHMPKLGGLRKPKKDSINTGWRNESFRGYADYMQTDEFQRA
                     IEELIAHGTSKKTAIMCAEAVPWRCHRSLIADALVTRDWEVRHIMSETKADRHKLASF
                     AAVENGELRYPRPDSGSLFP"
     gene            complement(7526..7975)
                     /locus_tag="VN647_05105"
     CDS             complement(7526..7975)
                     /locus_tag="VN647_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWG96444.1"
                     /translation="MEECRAAMNFALDCSVTMTWLFEHEAIKETDELLRRLSGPEKAV
                     VAQHWVLEVANVLLSAEHSKKKTPADTALFLNLLGKLAIESDVHTEHHATTTTLALGR
                     RHHLSSYDAAYLELAMRLGLPLATLDKNLRKAAQAEGIAVLPEKIPR"
     gene            complement(7951..8202)
                     /locus_tag="VN647_05110"
     CDS             complement(7951..8202)
                     /locus_tag="VN647_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWG96445.1"
                     /translation="MGITTVGLAEAKNKLSELIARVAEGEEITITRHDEAVARLVPAK
                     RSSREEARQAIEGIRALRAKHRVKLTTEDILAWKNVGRR"
     gene            complement(8728..9366)
                     /locus_tag="VN647_05115"
     CDS             complement(8728..9366)
                     /locus_tag="VN647_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HWG96446.1"
                     /translation="MAEAKYEVTAEGLNLREEPSIHGKIIEVLSKGDEVELISISGDD
                     YWYKVKASDSTEGWAAHKYLRKVVETPESDWPWLKVALAETGIHEFPGDADNPRVVEY
                     LHSTNLGSPSWNEDETPWCSAFVNWCIEKAGYEGTDSAWARSWLKWGKEIKEPVVGCI
                     AVLKREGGGHVGFYVGKTETAIRVLGGNQGDAVNESSYAKSRLLGYRLPGTA"
BASE COUNT         2363 a         2684 c         2347 g         2290 t
ORIGIN      
        1 ttttctttgg tcgggcccca tgacaaccgc atgggttatg acatccttcg ccgcgtgaac
       61 aggagtgcac ccacggatca tccccgtcaa ccaggacagc aaattccttg caaatgagcg
      121 gtcccatagg gagtcgattg cgttccaatt ggactttaga aaagaaactt tatcgttata
      181 tttttgggca tctcggagat cgttgccttc gtagaatatc cagacgacag acttgggatg
      241 tagcggaagc gcataacgtt tcaggacggc cagttcctgc tgcggaccat accctgattg
      301 tccaagattg gcgacagtct tttgcgtgag tttggccaac cgagtggtcg ccaacattga
      361 gccggggagc atcggtgatt caacatagga gtcaccgata accgcaatat ccgcactcgt
      421 aaaatcctca tcattgcgga acccgttctt gtcgtatttc acctcaaacg gctctgctgg
      481 gtgagaagga agacagagaa tctctccgat gttccctcga ctaaactcca ttttgacggc
      541 atggtagggc gctggcttga acaggagttc cggatcagga aggtagcccg gtcccccacc
      601 tatcctaccc gagatggaga aagtctgccg ataatcgatc agcttcacca aggcaggcaa
      661 ttctaccagt aacaatgccg ctccaaaggc aatcgtcatg agaacgaact ggtttcgaat
      721 ctcctctcgg ggaacagtag aaatcagcga atacccaccc cacaaaacaa gatatgatgc
      781 tatgaggcaa ctggacagat gccgtacatc tgaaatgcct ggcatgatca tcacggagta
      841 gatcgtgacg aatatgaacg atgccagacc aataacgatt cgcttgatat ttcgatcaga
      901 cataatcgtt acaccactcc ctacttctcc tagacttctg aataaatgac catttcccct
      961 tctccacctc aagactacta atcatgtcac cgaacttttc gaatatctaa atgaagaaag
     1021 ctttcatccc gccgatgcct gaactcagcc tctcctaccc ataatgtcat aatgaatggg
     1081 gcccaatcca ctagagttca ccctatacgc aaaagtgatc aggcccttcg gttctcactg
     1141 gaacgttatt gagagaagga acacaatatt ttcgagactt tgcagtttgt taagggatga
     1201 tgtccacact cagagagaac ggcggcactc tcgagtcaca gtcattttta ggtgggtcct
     1261 ggatgattac tacaaagagc gatgccgagg ccgtgccgcg aaaacacgac ttcggacctt
     1321 tttcattagc gagtttagaa tgcccggtat cgagatgctc caaataccag acggcacccc
     1381 actagaaaaa gctcaccgtg agactcaaca ggtacttttt tcgtccactg cacttcatac
     1441 aaactcaggg aatattccca tcgtgtttcg gcaacatgga gttccagcag ttgccgcttt
     1501 cgaggttggc cgcccatcaa cacgagaatt ccatgctcac tcctgttgat gcagtacacc
     1561 tcaccctgtt caatggtcac ctgcttccca tcgggtgatt cgcacaagct ataggcgcat
     1621 accttttgtt cgttcacccg ctcctctcgt cgtcgagaag gaaatgaaac cttcaggtct
     1681 ttcgtgacag caaccacagc ggcttcagta ttcagattcc ccatgcggca tacggacggt
     1741 tcatgcatga cttgctttgt tccaatattc atgaaacctc caaagttcga tcaagtggtg
     1801 cctacagcag agatggaggg agcaaatgtc atacctatag cactttgata atgcgtacat
     1861 gaattgctca tgaatccccc ttcaaactcc tcgtttaaga acgtatcgaa actaaacagc
     1921 gaagggatat ggattcatca tttcgatgta ttgtgatgat agcttgttga ttctaaatgc
     1981 aatcaaacgg cgaatggaaa agcatacaca atagaagctg gcgtccttca ccggaatgta
     2041 gggtcaaggg agcgttgaat gccaattcaa acgaagacaa gcgaaggatg ggtcaactta
     2101 tgtttttgac caaaatccta tgcagcatgc gatacaaggg caggtcaccg aattgcagcc
     2161 tgatgttcga catctcttag tcgggtaagg taaagagcct atcgaataac ccaacacggt
     2221 tcatctcaca attcagcttc tccacgcgaa ggccgagcaa acggatgagt tttctgtgtg
     2281 ggttttcacg ccgatcgaga aagggcagca tcagcttcat ggcttcccgt ctcaatatcg
     2341 ccgactcatc agtcgccata gccaatgtat gggcacgtga cttggtcaca aaatccgcaa
     2401 aacgaacggt cagcacgacg gttcgaaacg aatgaaatcc ctcgtgatgc aggcgatcaa
     2461 tcacgcctcg aacgagccca ttgagttgat tgagaatcgt ctgactatcc agcgtatctg
     2521 attcgaacgt ttcctgttcc cctatagatt ttagctccga atattcctcg accggtgagt
     2581 catctcgtgc gcgcaccttc tcatagagat ccacgccgcg tttccccaac agggcttcga
     2641 gctgatggag agtcagcact cttagatcct caacaatcct aatattcttt gctttcaaga
     2701 gaccttccgt tttaggcccg ataccaggga tgacccggat cgagagtgga gcgagaaacg
     2761 cctcggcctc ctccgctcgt acgacaatga gtccgtcagg tttgtgccat ccagaagcga
     2821 ttttggcgat caacttattc ggcccaatac cgattgaggc cgtcagctgt tcttccgagt
     2881 ggatctccgc cttcagacga cgacagacgc gttctgcttc atcatacgat cctgtccaac
     2941 tcacatcgcc gtatacttca tctacactgg cttgttccac acaaggaatg aagcgccgta
     3001 cgattcgcat cacttcctct gaaacccgtg catatttttg catgtcgacc gatacaaagg
     3061 tcaccggcgg ctttcccgtc tgacgagcgg cctcagataa gcgccacgca gtcgagatcg
     3121 gcgtcgccga gtgaattccg tacgcacgcg ctagatagtt cgacgtggac gcaaccccgc
     3181 gacctcttcc gcccagcgga tctgcgccaa cgacgagggg gactccgcga aaagccggag
     3241 tatcgcgctc ctcaatcgcc gcgaagaatg catccatatc taagtgtgcg ataaccctat
     3301 gcacttttca tctcatgccc ccttggatac catcaagcag ctgatgggcc acacggaaga
     3361 tgcttcaagg gacagtcatt ttcaccgtag cttttcgtgc caaggatgac atacaattgc
     3421 atccctgttg agaagaggtc gattcattca cacgatcatt gctcacaagt cagaatcgtc
     3481 aacgagagga ggcatgtcgt gggttatcgt tggtacaatc tcatcggatt tctgatgttg
     3541 ctttccgctc tcttcctaaa tgaggtcgtg tatttgaacg tgtggtacga tctcaatttc
     3601 tccgtgattg ttccggctct cgtgtatctc ggtgttggac tttggctctc actgtgggga
     3661 gggccccgtc gtcagaacgc atgatgtcgt tcatccacta acttttcaag gtctaggacg
     3721 gacttaaatc tggggcgtga catagggttc caattcagtt caacagaaca ttcatttagg
     3781 agttcatcga acgcagggca aaaaccatca ctagaaagga gacaatccat gggtatgacc
     3841 tttatcaatt ggattggatt cgttatgttg ctgctggcgt tccttctcaa tgaagcattc
     3901 ctcgggataa acgcctggta cgagctcaac ataatggcgg ttcttgcggg tgtcgtgtgg
     3961 ctggtcgttg cggccgcatt atcaatatgg ccaagccata gagatcagag agcctgatca
     4021 attctctttc gccttgccaa aagctcgagc gcagaggtca ggatatgtct tctgcgcttt
     4081 atgactgtga agctcgctta cttctcataa aagtaagaaa agtgccttca ctgaatgcca
     4141 tccccgccaa gaaaacaatc acttgtgtga tcattcacca gcccgactgc ctgcatatag
     4201 gcatagacga tcgtgctgcc tacgaatcgg aagccgcgct ttttcaggtc ttttgacaag
     4261 gcgtcgcttt cagtcgcgat ggccggaacc tggctcaggg ttttccaacg attgattctt
     4321 ggcttcccgc cgacaaatcg ccagacatac cggtcaaacg atccacactc cttctgcacg
     4381 gtcagaaatg cctgcgcatt cgtgacagcc gcctcgatct tcaatcgatt gcgaataatg
     4441 cctacatcct gcagcagcgc ggcttccatc cggcgtgtaa acctggcgat tacagcgggg
     4501 tcaaatccga caaagacctt acggtatcct tcgcgacgca gcagaatcgt ctcccatgtc
     4561 agacccgcct gagccccttc aagcaacagc atctcgaaat gcttgcgatc gtcatgcacc
     4621 ggcacacccc actcctcatc atggtaccgc atgagatgcg gtttcagacc gacccagccg
     4681 caccgaatct ttgtgtctga catggcgaga atggatacct cggcgagatt gtatcagatc
     4741 ggcaagtcgc atatttcatt atagttcatt atagatagtc cacccggttt ggtccttgag
     4801 caattttcag ggttgatctg ttacgtacaa atccactacg attctcttat tcggctaact
     4861 caggatgaga ggggtttcta gatcgttaaa gaagaattgt tccatgcaat gtaaccacca
     4921 tcccccaacc cacccccaac agaggaggta gtcatggcag ccaaacagta caaacagctt
     4981 ggatgtctgg acgtcgaacc gtccgccggc tgtgcatttc aggtacgaac cgagaccgag
     5041 gcagaactga tgcagctcgt cgcgacccac gccaaacagt gtcataagct ggactcgatc
     5101 ccgcaggaga tggctgccaa agtaaagtca gctatcaaaa cagtcacggt cacggtttag
     5161 cgaagctcga tacagagagg gacttcctcg agtacggtct cgtagcgggg aaggctcccc
     5221 tctctcaact ccaagtcgtt atcaaggagg acttatgggt tggaagtcag tgttcactat
     5281 tgccgccatg ctgctactga gtaccagcgg gtgtagttac ctgttttatc cccacgcaaa
     5341 ggaatttacc gagaaagcga agggagcaac gggtgtcgat acgctcatca atctgaccaa
     5401 tatggcggaa gccacggcca aagccgccaa gggcggcaag ggagccgatc aacccttcga
     5461 cgatctgcac aaccagtttc atgcgatcga caacagcatc tgttgcgtcg agaaatcggt
     5521 caaggaacag ccggcctacg cactggctgt cacacacaat aaagaactca agacgatttt
     5581 caaacggcta tggaaattca agaatgatca gcctcagcgg gatcaacact tggacctctt
     5641 tgtgtccgaa ctccaggaga tgcgccaaac tctggaaacc ctgaggtaac ttcatggcag
     5701 ggccatcgcc tgaacaagtc atcgatagcc aacgacaaga ctggaaccgt gtcgccggcg
     5761 ggtgggagaa atgggatcgc ttcttcgatg agcagatggc gtttctcaat caccggctcg
     5821 tgggcgactc cagattgcac agcggtcttc gagtgttgga tctgggatca ggcacgggct
     5881 atccggcact cctcgccgcc caagcagtcg gagcgaacgg cagcgtggtg ggcatcgacc
     5941 tcgccgagca tatgctcacc atagcggaga gaaaggccac gcgattgggc ttacaaaacg
     6001 tgatgttccg ggtcggcgat gtgaccactc tcccgttcga atccgcttcg ttcgatgccg
     6061 tcaccagccg cttctgtctc atgtttctcc ccgaaattcc caaagctgcc gccgaaatcg
     6121 cacgtgtcct gaaaccggga agctggctcg cagcagccgt ctggtccgct ccggaaaaaa
     6181 atccctccat cggtctctca atgacagccg tcaagcaggt aatcgacctg ccgccgacgg
     6241 acccgctggc accaggcatc tttcgtctcg ctcaacccgg cgacttcgcc ggcatgttga
     6301 agagagccgg ccttatagac attacggacc aggagtttct tggggaatgg tcctacggct
     6361 caaccgatga atattatacg agcctgatgg agatcgccgc cccggtccaa aatctcatga
     6421 cgacgctgtc ggagccacaa cggcaggagg tgcgacgcct tatcatcaaa gctgcttcgc
     6481 agttccagcg cggagctcgc atcacgtttc cgatcgccgt gcggatggtc gcggggcgca
     6541 agcccggatg aggcatgccg acgaagaacg acggcttcaa aaacttcatc ttggatcagc
     6601 tgacggagct gaagggggtg actgcacggg ccatgttcgg cggctacggt ctctatcaga
     6661 gagccacgtt gttcggcatc attcataaag gccgtctcta cttcaaagtg agcacgacca
     6721 ccgttcacaa ttacatggat catggcatga aaccgtttcg acccaatccc aagcagacac
     6781 tcaaatccct ttatggggta ccgattgatg tgattgaaga tgcggaagcg ttgacccaat
     6841 gggcgacaga agcagtcaag agatgatcag accatgtccc acattctctg gaccatcggc
     6901 cattcgacaa gaccgatcga cgaattcgta tcgcttctga aagcgcatgg catccaacag
     6961 ctcgtcgatg ttcggactgt tccccgctca cgccacaatc ctcaatacaa taacgaagca
     7021 cttttaaaga gcttgcggga tgaggacatg tcgtacaagc acatgccgaa gctcggtggg
     7081 ttgcgcaagc ccaagaagga ttccatcaac accggctggc gcaatgaaag ttttcgcggc
     7141 tacgccgatt acatgcagac ggatgaattt caaagagcta ttgaagagct gatcgcgcat
     7201 ggtacgagca agaagacggc gatcatgtgc gcggaggccg tgccctggcg gtgccatcga
     7261 tccttgatcg ccgatgcact agtgacgaga gactgggaag tccgacatat catgtcggaa
     7321 acgaaagcag accgacacaa gcttgcttcc ttcgccgcgg tggaaaacgg ggaactgcgg
     7381 taccctagac cggacagtgg aagtctattc ccctagcgat gtccatgccc tgctcttctt
     7441 catcgaaacg ggtgaaaagg gttctatggc tcctcggccg gagctttgaa gaggcgcggc
     7501 tcgatttgtt tccggcccaa atgccttatc tgggtatttt ttccggcaag acggcgatac
     7561 cttcggcttg cgccgccttt cgcaagttct tatcaagggt ggcaagcgga aggccaaggc
     7621 gcatcgctaa ctcaagatac gccgcatcat agctgctcag atggtgcctt ctccctaacg
     7681 caagcgtggt ggtcgttgca tggtgttccg tatggacatc cgactcaatg gcaagcttgc
     7741 cgagcaaatt tagaaacagc gcggtgtcag ccggcgtctt cttcttggaa tgttcagcac
     7801 tcaacagcac gttggcaact tcaagaaccc aatgttgagc caccacagct ttttccggac
     7861 cagaaagtct cctgagcaat tcatcggttt ccttgatggc ctcatgctca aacagccacg
     7921 tcatcgtaac tgaacaatca agcgcgaagt tcatcgccgc ccgacattct tccacgcgag
     7981 aatgtcctcg gtggtcaatt tgacacgatg cttggcgcgc aacgctcgaa tcccttcaat
     8041 cgcttgtctc gcttcttccc ggctcgatcg tttcgcaggc accagtctgg caacggcctc
     8101 atcatgccgc gtgatggtga tttcctcacc ttcagcaaca cgggcgatca attcggagag
     8161 tttattcttc gcttcagcaa gtccgactgt cgtaatgccc attgtcacaa ccctccatac
     8221 ccagacacat taatcaagct agacactggc tagattaaca cagcaccgaa catgtgacaa
     8281 gccatgcaat attcagctgc gtgagacgat gcgtccttct ttggtgactt cgcggagagt
     8341 ggcggcttgc agttagcggg aggttgcggc gcaggagcga tcggccaatc ttgcacggcg
     8401 taggagtacg tggcgaatga tatcggatgg ccggacatgg gccatcagac ggcgggagta
     8461 ctccgccgcc gaagcggctc ccgaaccagc ggatgtccac tggaccgccc ctgtcgacca
     8521 actcgtgaat agcttcctgg gatagacgtc aattaatcgg acattcctta tttgttcatc
     8581 tggtctcccc agaattaatc agacgccagg aagaatttgc ctcttggcag catggtgcgg
     8641 actttcgaca aaccacctca ccaccatact ggcaatggaa gtatgaatca atccgagctc
     8701 cctagatctt gctttcaacc ttacttttta ggctgtcccc ggcagtcggt atcccagcaa
     8761 gcgagacttt gcgtacgacg actcattgac tgcgtcacct tggttgccgc caagcacacg
     8821 gatagcggtc tcagtcttac ccacatagaa cccaacgtga ccgccacctt ctcgcttgag
     8881 gacggcaata caaccgacaa cgggctcctt gatttctttt ccccacttca gccatgacct
     8941 ggcccatgct gagtccgtgc cttcgtagcc ggccttttca atgcaccagt tcacgaacgc
     9001 cgaacaccat ggggtctcat cctcgttcca tgatggacta ccgagattcg tcgaatgcag
     9061 gtactccacg acacgtgggt tgtcggcatc gccgggaaac tcgtgtatac ccgtctcggc
     9121 caaagcgacc ttcaaccaag gccaatcact ttctggagtc tccacaacct tacgcaaata
     9181 cttatgcgct gcccaccctt cagtgctgtc ggatgccttg accttgtacc agtaatcatc
     9241 gcccgagata ctgatgagct caacctcatc acccttactg agcacttcaa tgatcttgcc
     9301 gtgaattgag ggctcctccc taagattcaa cccttcggct gtaacttcgt atttcgcctc
     9361 tgccatactt ttcttcctga acaattccgc tttcgacgta gaagacaaga ttccagcctt
     9421 tcttttcttc ttcgaaatcc gacgtttttt tgaagatcct atacgtgcca tcacgacctc
     9481 tctccgtgtt tcattaccat gttcatctgc cgaggtgatt cgtaactttt acgtcgtgct
     9541 ctgcagactg gtgcttggag cgctcccacc tctttagctt gcgggtgccc tcctcatagg
     9601 gtcagtagtg gcgactttat cgcgactact gatcccagtt ttttgcaaag gacgttgcaa
     9661 accgtatcgt catgagtatt tact
//
LOCUS       DATNDD010000211         5273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3890837, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000211 DATNDD010000000
VERSION     DATNDD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890837"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..454
                     /locus_tag="VN647_05120"
     CDS             161..454
                     /locus_tag="VN647_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96447.1"
                     /translation="MVGELGQLGDLAQTVSLDGHRLAKELRNRVGNLPALFARHVPQA
                     RQMLRKLLDGHILCEPIMQDGRPGYRFTATGTFDRFVTGVQAIGNFDGGGQGS"
     gene            complement(433..508)
                     /locus_tag="VN647_05125"
     tRNA            complement(433..508)
                     /locus_tag="VN647_05125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(473..475),aa:Ala,seq:cgc)
     gene            1012..1674
                     /locus_tag="VN647_05130"
     CDS             1012..1674
                     /locus_tag="VN647_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96448.1"
                     /translation="MLHAPISFPDDRAQSIVNQEFQSERFFYPKAFHERGKVLPQIPQ
                     IYHQLRILLHKKRWALRFTQIVRGWLTLGPFRKSAIRYYQRRNHVRPMQVERHDLFPQ
                     LEVDPVVNSLNERGYANGWKIPEGYVARIIQYTEDTKFLKYWNPHRECEAIDQIARNE
                     KLVEIARQYLGSEPILWLTQLKWSFGNKADKERQGLLSSRNVPVQYDEDAFHYDTLDF
                     KL"
     gene            complement(2062..2385)
                     /locus_tag="VN647_05135"
     CDS             complement(2062..2385)
                     /locus_tag="VN647_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWG96449.1"
                     /translation="MHASHPEPTDQFTVEISRDLEGIVPIFLGNRKKDARILRDALVK
                     QDFRTVQTLGHRMKGDGGGFGFDRITEIGAAMERAAKLEDPLTIEEHIGQLEDFLQRV
                     TVVYR"
     gene            complement(2388..4673)
                     /locus_tag="VN647_05140"
     CDS             complement(2388..4673)
                     /locus_tag="VN647_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96450.1"
                     /translation="MSRSRHPHGGLQDHAAVTMISSRSSRSTTRRTTASLKRKPSERT
                     ALPFEQSATTRLKQYQETLRRLAQSEALNSGNISRVFRTISEACCSLLQVKRASVWLF
                     TEERTAIELIDVCTSGRRHSNPTFVLQSSQWLVYLRSLTRKNRRIAVHHVFRDSRFKE
                     LAPSYASRFHVEAVLSAPIRQNRRLVGALCAESVGAPRRWSRHEEHLVGLLATMTTLA
                     LEAAERHEVEQALRVAKEAAEVANRAKSEFLASMSHEIRTPMNAIIGMADLLWDTPLS
                     PDQRKYLRIFRRAGGTLLNLINDILDLSKVEAGRLELESIEFDLNEVIDKAIDIMAMR
                     ANEKGLELACHVDQNVPCHLVGDPTRLTQILINLIGNALKFTDEGSIVVRVTNDLDRN
                     IAGAVRFAISDTGIGIPSDHLVSIFERFTQAHASTTRRYGGTGLGLAIAKRLVEGMNG
                     RIWAESTMGTGSTFYCSVLLEAQTESSHQKSATPINLAGVRVLVVDDHPTNRLILREL
                     LSAWGAQVTEASDGMTALDELRQASERGHAYELLLLDCRMPEMSGFRIVEQLKSSPNG
                     GSPTIIMLTSDHWADDIARTYDLGLGGYLIKPIRRSDLSQTIGIALGRTKGTPQAGVE
                     VIASASTVSTRALRVLLVEDSPDNQLLVRAYLKQTDYVLDVAEHGAVALDKFKSAHYD
                     VILMDVQMPVMDGYAATKAIRSWERDHDLPPIPIVALTALALKEEGARIFEAGCNTHL
                     TKPIKKSTLLEVLRAYKEHIV"
     gene            complement(4710..>5273)
                     /locus_tag="VN647_05145"
     CDS             complement(4710..>5273)
                     /locus_tag="VN647_05145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWG96451.1"
                     /translation="QLQEANQMLLRLSCLDGLTGITNRRQFDDFLDQEWRRAARESTP
                     LSLIMFDIDRFKTYNDAKGHTSGDECLKQVAAAISSSVNRPGDLVARYGGDEFVTVLP
                     GTGIDGAAQVAESLRRRVESLGMKHADGELVTISVGYASIVPNRNASPTDLIKTADQA
                     LYQAKQEGRNRIKSASILSLVQDSHGD"
BASE COUNT         1205 a         1333 c         1476 g         1259 t
ORIGIN      
        1 acacaagacc atcatcggag aatcaactgg tgaattcgat gcaggaattc tttaaaaacc
       61 gcagtttgtc aacaaactgc tagttgagtt aatcgcgaat ggacggggga ccgacactgt
      121 cattgactcg ctacatcagg aagaagccag gaagaaagtt gtggtagggg agctcggcca
      181 gcttggggac ctggcacaga cggtctcact cgacgggcat cgccttgcga aggaactaag
      241 aaaccgcgta ggtaatctgc ctgctctgtt tgctcggcat gtaccgcaag cccgtcagat
      301 gctcagaaag ctcctggatg ggcatattct gtgtgagcct attatgcagg atggcaggcc
      361 cggctaccgc tttacggcaa ccgggacctt tgatcgattt gtcactggcg ttcaagccat
      421 aggcaacttc gatggtggag ggcaggggag ttgaaccccc gacccctacg ttgcgaacgt
      481 agtgctctcc caactgagct agccccccac ccgtgggata tggggaccgt gaccactaag
      541 tcccgcaggg ttttgctgca tataagcatg cagtcttgtt gctgcggtcg aagaacgggg
      601 aggaatatcg gatcacgtta cccgggtttc attatacaca acccgttgct gagactctac
      661 caatacattc tcacacacga gcccgcgtta acagtcttcg atattggcag ggttagcgca
      721 attcgatcga gaccgaattg ggaaaggatt gaaagtaaat gatcaacacc gaccaaaatc
      781 ttttcatgtc tccgggcttc gtatcgcttg gtatcaacga acatgctgag aacgatcgaa
      841 gaatattttt gcctcatcat acttttgggg tattccgttg tctgagtaat accgtgttag
      901 ggtgtcgacc ttgtctttgg agacgctggt tggcaggagg aagtagggcg tccaagtgcg
      961 tgtaagatgg cagggatgcc agttgccgta tgagctacgt atgttagcgg catgcttcat
     1021 gctccaattt catttcccga tgatcgtgcc cagtcgattg tgaatcaaga atttcagagc
     1081 gaaaggtttt tctatcccaa agcgttccat gaaaggggaa aagtgctacc acagattcct
     1141 cagatttatc atcagctccg gatcctgctt cacaagaaac ggtgggcact tcggtttacg
     1201 caaatagtcc gtggttggct gacactaggt ccattcagga aaagcgcaat ccgctactat
     1261 caacggcgca atcatgtccg acccatgcaa gttgagcgac acgatttatt cccacagctt
     1321 gaagtcgatc cggtggttaa ttcgctgaat gagcgaggat atgcaaacgg atggaagatt
     1381 ccggaagggt atgtggcgcg gattattcag tatacggagg acaccaagtt cttgaaatac
     1441 tggaatccgc atcgagaatg tgaggcgata gatcagatcg ccaggaacga gaagctcgtg
     1501 gagattgccc ggcagtactt gggctccgag ccgattttgt ggttgacgca attgaaatgg
     1561 tctttcggta acaaggcgga caaagaacga caaggtttac tatcgagtcg taacgtaccc
     1621 gtacagtatg acgaggatgc atttcactat gacacgttgg atttcaagct ttaaccattt
     1681 tcatctatct gaccgacgtg gacgcctctt gcggtccgca tattctcatt gagaatgcgc
     1741 ataaaacaaa aagatttgga gaaatttgtc ggattattct gagtgatacg gtcgctcagc
     1801 aaaggttcgg cgatcgcatt aagccgatgc tgggcgaaaa ggggaccatg gtgttcgagg
     1861 aaactagcgc ttttcataag gccagccgat gtcagaccaa gcgactgatg ctttcgatcg
     1921 actatgtgtt gcagcgatca cctcctccgg aacgtccgat tatggcttga tgatgaggaa
     1981 ccattcgaat ggctgacgat ctggaaactg ctgtgggaag ccaatgctgg tagttggtcg
     2041 gttgaaacgg accgctattc tctagcggta gacaacggtc acacgttgga ggaagtcttc
     2101 cagctgtccg atgtgctctt cgattgttaa ggggtcctcc agttttgccg cgcgttccat
     2161 cgccgctccg atttcggtaa ttcgatcgaa cccgaagccg cctccatcgc ctttcatgcg
     2221 atggcccagt gtctgaacgg tcctgaagtc ttgtttgacc aaagcgtctc gaagtatccg
     2281 ggcatctttc ttccggttcc ctaagaagat cggtacaatc ccttcaagat cgcgactgat
     2341 ctccaccgtg aattgatctg ttggttccgg atgggaggcg tgcattgtta gacgatgtgt
     2401 tccttgtacg cccgaagaac ttccaataac gtggatttct ttatgggctt cgtgaggtgt
     2461 gtgttgcagc cggcttcaaa gatgcgtgct ccttcctcct tgagggccaa tgcagtgagg
     2521 gcgacgatcg gtatgggtgg aagatcatga tcccgttccc atgagcggat ggcctttgtc
     2581 gcggcatagc cgtccatcac aggcatttgc acgtccatca agatgacgtc gtaatgcgcg
     2641 gacttgaatt tgtccagtgc gacggcgccg tgttctgcca cgtcgaggac atagtcggtc
     2701 tgcttcaggt atgcccggac cagcagttgg ttgtcggggg aatcttcaac caataaaacg
     2761 cgcaaggccc gcgtcgaaac ggtcgacgcg gatgctatga cttccacacc cgcctgcggc
     2821 gttccttttg tccgaccgag ggcgatcccg attgtttgcg aaagatcgga ccgccggatg
     2881 ggtttaatca gatacccacc gaggccgaga tcatacgtcc gtgcgatatc atccgcccaa
     2941 tgatcggagg tcagcatgat gatggtcgga gagcccccgt tgggcgagga tttcagctgt
     3001 tccactatac ggaagccgct catctcaggc atccggcaat cgaggagcag aagctcgtac
     3061 gcatgccctc gttcggatgc ctggcgaagt tcgtctaatg cggtcatccc gtcgcttgct
     3121 tccgtaacct gagcccccca ggcactcagc agttcacgga gtatgaggcg gttggtggga
     3181 tggtcgtcca cgaccaggac tctgactccg gcaagattga tcggtgttgc ggatttctga
     3241 tgggacgatt ctgtttgagc ctcaagcagg acagaacagt agaaggtgct tcccgttccc
     3301 atcgtactct cagcccagat ccgcccgttc ataccttcga cgaggcgttt ggcgatcgca
     3361 aggccgagtc ctgtccctcc ataccgcctg gtcgttgagg catgtgcctg cgtaaaacgt
     3421 tcgaaaatcg aaacgagatg gtcggaagga atgccgattc ctgtgtcgct gatggcaaac
     3481 cgaacggcac cagcgatatt ccggtcgaga tcgttcgtga cacgaacgac aatggaacct
     3541 tcgtcggtaa atttgagggc attgccgatc aaattgatca gtatctgtgt cagccgcgtc
     3601 gggtcaccga ccaaatgaca gggcacattt tgatccacat gacaggcaag ctccagcccc
     3661 ttctcattgg cacgcatcgc catgatatcg atggccttgt cgatgacttc gttcaggtcg
     3721 aattcgatgg attccaactc caagcgacca gcctcgactt tggaaaggtc caaaatatcg
     3781 ttgataagat ttaaaagagt gccgcccgca cgcctgaaaa tacgcaggta ttttctttgg
     3841 tccggtgaga gcggagtatc ccaaaggaga tcggccatgc cgatgatggc attcataggg
     3901 gtgcgaattt catgactcat actggcaaga aattcactct tcgcgcggtt ggccacttca
     3961 gcggcttctt tggcgacacg gagggcttgt tctacttcat gccgttcggc tgcttctaaa
     4021 gcaagggtcg tcatggttgc gagtaggccg acaaggtgtt cttcatgccg gctccaccgt
     4081 cgaggggcgc cgaccgactc ggcacagagt gcgcccacga gccttctatt ctgcctaatc
     4141 ggtgcactca acacggcctc gacatggaag cgcgaggcat acgacggggc cagctctttg
     4201 aatcgtgaat ctcggaaaac atggtgaact gcaatgcggc gatttttacg ggtcagcgat
     4261 cgaagataaa caagccactg tgaggactgg agcacgaagg tagggttgga atgtcgccgg
     4321 ccggatgtgc acacgtcgat tagctcgatg gcagttcgct cctcagtaaa cagccagacg
     4381 ctggcgcgtt tgacttgcaa caagctacag cacgcctctg aaatggtccg aaagacgcgg
     4441 gaaatattgc cgctgttcaa cgcctcgctt tgcgccagtc tgcggagcgt ctcttgatac
     4501 tgcttgagcc gcgtggtcgc actctgctcg aatggaaggg cggttcgttc ggagggcttc
     4561 cgcttgaggg aagcggtggt tcgtctggtt gtcgagcggg aagaccgaga ggagatcatg
     4621 gtgactgcgg cgtgatcttg aagtcctcca tgtggatggc gcgaccgtga catggggaat
     4681 caatactcgg cgaatagttc tgagcgcctc tagtctccgt gtgagtcttg gaccagcgac
     4741 aaaatactgg ccgatttgat ccgattgcgt ccctcttgtt tcgcttgata gagagcctga
     4801 tccgcagttt tgatgagatc cgtaggagaa gcgtttctgt tgggaacgat gctggcatat
     4861 ccgacgctga tcgtgacgag ttctccatct gcgtgtttca tcccgagaga ttcgactcgg
     4921 cggcggaggc tctccgctac ctgtgccgca ccgtcgatac ctgtgccggg cagtaccgtg
     4981 acaaactcgt ctccgccata gcgggcgacc aaatcgccgg ggcgattcac tgagctggag
     5041 attgccgcag caacctgttt gagacactca tcgccggacg tatgaccttt ggcgtcgtta
     5101 taggtcttaa atcggtcgat atcgaacata atcagcgaca aaggagtcga ctcgcgcgcc
     5161 gctctccgcc attcctggtc caaaaagtca tcgaactgac gtcgattggt gatacccgtc
     5221 aatccatcca agcaggaaag acggagcaac atctgattgg cttcttggag ttg
//
LOCUS       DATNDD010000212         3992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2544485, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000212 DATNDD010000000
VERSION     DATNDD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544485"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VN647_05150"
     CDS             complement(<1..1191)
                     /locus_tag="VN647_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG96452.1"
                     /translation="MTNAVTRHPEERELLHAAFRSFDEAAQTLQQSYGALTARVEQMD
                     LELAQSNEALRRQLGDNEAMRMHLDGVLESLSTGVLVTDDCGTISRSNRAAETLLSTT
                     DAELRNRRATDVLAGAGLGVCDRPQRIGQTVVSIGRVPLRNDSGERSGHLILFQDITR
                     IYQLEEQLQRKERLAAMGELIGRIAHEIRNPLGSVELFASMLQRDLAEHSSSKRYAQQ
                     ISEAVQAMDRLLANLLLYTRPVRMARDWHLAEPLIGESIKLAAHALTRVPVEIRVDIS
                     REVRSIWCHDGQLKQVLVNLIVNAVQAMPNGGVLAIGFSPESPQTLGMAAVRLTVRDS
                     GLGIDPVHRSRIFDPFFTTKDEGTGLGLAIVYSIVEAHQGRIDVESTVGQGTSCSIIL
                     PHPST"
     gene            complement(1188..3194)
                     /locus_tag="VN647_05155"
     CDS             complement(1188..3194)
                     /locus_tag="VN647_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96453.1"
                     /translation="MQLLPDDGPRQEGQSSGIDGLAWQEGRSAYRKNAWPEAQRFFGK
                     IVKEYPESPLVPSAKAFLVELLLRDDVSGQTRSEAIAEYKKLLRDHPQSSNARRAEWR
                     IADLYVEQGAFQEAQAFYEQAMAHSVNGSFDRNRALLGLGYTFMAMGKWSDAEHAFAD
                     LRKGSEDEQLLQGATLGLAHALFREQRLSEAQPIFDIGYRRWPHLLRNDPLSLQRYAL
                     AQVKLHHEASARELMLLFYNLYPRHEYAPTALLHVAGGLRTGAKQPLAEFVYALIPSL
                     YPDSASDTTAKLRLAALRAENMLPAGSNSVGLTVSAMIHEVPIPDQTEASYRSLLEEI
                     ATREVGNPMGSEALYYLGKAYEYASDMNRALQTYKEITLRVADKNDPWALKASERLSA
                     LLTPWIEAAIASRDDLTVVSLFHRHGAMAEQRYARSPLLPDIAESHRRLGFSREAVRL
                     HQLVIKSHSDSALLESALIGLGKIYLDQRDPQAARKVLERYRFQFPLGKYEGEVVHLL
                     IDAMQQLRDLQGLLHLCRTWLLRHSVHPERPAMYVQLAKTLGELDKLDESALAYGEAF
                     KAGAEQSPDMLVSYADTLSRLNRHERAIAVYQSVLEKKPNVSQADWARLQAAHHWAAL
                     KQYDRATVALAELDVTDDPMLNRLSSSLRSSLRTAGRSGRSEGL"
     gene            complement(3202..3381)
                     /locus_tag="VN647_05160"
     CDS             complement(3202..3381)
                     /locus_tag="VN647_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96454.1"
                     /translation="MPYRRVSMPTTISSIGILDRRLDMVLLAGHRGGPCASFSQYFNH
                     NTGRTNDLDTESSTG"
     gene            complement(3486..>3992)
                     /locus_tag="VN647_05165"
     CDS             complement(3486..>3992)
                     /locus_tag="VN647_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWG96455.1"
                     /translation="RERRSDIPLLIDHFLTRFNQTKHTEVSGLAPDALHLLTEYDWPG
                     NIRELENMIERLVVLKKRGVLSVGDLPEKICRKPSGSELKEQFIRFTEDGINLSREVE
                     QYEKHLIMEALRKANGVTSRAAQLLHLNRTTLVEKLKRKGVDPRSHLETLPLWPRPSS
                     QKIDDLAT"
BASE COUNT          851 a         1159 c         1131 g          851 t
ORIGIN      
        1 tgtcgaagga tgaggcagaa taatcgagca tgaggtgcct tgcccgaccg tgctttcgac
       61 atcgatccgt ccttggtgcg cttcgacgat cgaatacaca atcgccaggc ccagaccggt
      121 tccctcgtcc ttcgtcgtaa agaacggatc gaatatgcgc gagcgatgga ccggatcgat
      181 acccaggccg gaatcgcgta cggtcagccg aacagcggcc atgccgagcg tctgcggcga
      241 ttccggggaa aaaccgatcg ccaagacacc tccgttcggc atcgcctgaa ccgcattgac
      301 gatcagattc acgaggactt gcttcagctg cccgtcgtga caccagatcg aacggacctc
      361 ccgactgata tccactcgaa tttcaaccgg cactcgggtg agcgcatgag cggccaactt
      421 gatcgattcg ccgatcaatg gctcagccaa gtgccaatca cgagccatgc gaacgggcct
      481 ggtgtaaagc aagagattgg ccagcaagcg atccatcgcc tggacggcct ccgagatctg
      541 ctgggcgtac cgcttggagg acgagtgctc ggcgagatcg cgctgcagca tggacgcgaa
      601 gagctctacg ctgcccaagg gattcctgat ctcatgggcg atccggccga tcagctcgcc
      661 catggctgca agcctttcct tgcgttgcag ttgttcttca agctgataga tgcgagtgat
      721 gtcttgaaac aggatgagat ggccggaacg ttctccggaa tcgttgcgga gcggaacccg
      781 accgatagag acgacggttt gtccgatgcg ttgcggacga tcacatacac cgaggcccgc
      841 acccgccaag acgtccgtcg cacgacggtt ccgcagttcg gcatccgtgg tgctcagtaa
      901 ggtctcggcg gcgcgattgc tgcgactgat cgtcccgcaa tcgtccgtca ccaacacccc
      961 ggtcgacaag gactcaagaa ccccgtccag atgcatgcgc atggcctcat tgtcgccgag
     1021 ctgtcgacga agtgcctcgt tgctctgcgc caactcaaga tccatctgct ccacgcgtgc
     1081 cgtgagcgcg ccgtatgact gttgcaacgt ttgggcggcc tcatcaaaac ttctgaatgc
     1141 ggcatgcagc agctcccgtt cctccggatg cctggtcacg gcattcgtca tagcccctct
     1201 gatctccccg aacgaccggc cgtccgcaaa ctcgatctaa gggacgacga cagtcgattc
     1261 agcatcggat cgtccgtgac atcgagctcg gccagcgcga cggtggcacg atcgtattgt
     1321 ttcaacgccg cccaatggtg ggcggcttgc agacgcgccc aatcggcttg agacacgttc
     1381 ggcttctttt ccaacactga ttgatagacg gcgatcgccc gttcatgtcg attcaatcgc
     1441 gacaaggtgt ccgcatagga gacgagcata tcgggcgatt gctccgcgcc ggctttgaac
     1501 gcctctccgt aagccagtgc ggactcatcg agtttgtcca gttcgcccag cgtcttggcc
     1561 aactggacat acatggccgg tcgttcagga tgaacggaat ggcgcaagag ccacgtacgg
     1621 cacagatgga gaagcccttg aagatcccgc agttgctgca tggcatcgat cagaagatgc
     1681 accacctccc cttcatactt tccgagaggg aactgaaacc ggtatcgctc caacactttt
     1741 ctggcggctt gaggatcccg ttgatccaga tagatctttc caaggccgat caaggcagat
     1801 tccagcaacg ccgaatcgct gtgcgattta atgactaact gatgcagccg tacggcttcc
     1861 cgggaaaatc ccagacgccg gtgagattcc gctatgtccg gcaacagagg ggaacgcgca
     1921 tagcgttgct cggccattgc tccatgtcta tgaaacaggc tgacgaccgt caggtcgtct
     1981 cgcgatgcaa tcgccgcctc gatccacggc gtcagaagag cggacaggcg ctcagaggct
     2041 ttcaaggccc atggatcgtt cttgtcggcg acgcgaagcg tgatctcctt gtaggtttga
     2101 agcgcccgat tcatgtcgct tgcatattcg tatgctttgc cgaggtaata cagcgcctcg
     2161 cttcccatcg gattaccgac ttctcgagtc gcaatctctt cgagcagcga gcggtaggag
     2221 gcctcggtct ggtccggaat cggcacctcg tggatcatgg cgctgaccgt gagcccgacc
     2281 gaattgcttc ctgccggcaa catgttctcc gcacgaagcg cggcgagccg gagtttggcc
     2341 gtcgtatccg atgcgctgtc cggatagagc gaggggatca gagcatagac gaactctgca
     2401 agcggctgtt tggcaccggt ccgcaacccc ccggctacgt gcagcagagc cgtcggcgca
     2461 tactcatggc ggggatagag attgtaaaag agcagcatga gttctcgagc ggacgcctca
     2521 tgatgcagtt tcacctgcgc gagggcatac cgctgaagag acaagggatc gtttctcaga
     2581 aggtgcggcc atcgccgata gccgatatcg aagatcggct gtgcttcaga caatcgttgc
     2641 tctctgaaca gcgcatgggc caaccctaat gtcgcgcctt ggagcaattg ctcgtcttca
     2701 cttccctttc gcaggtcggc aaacgcatgc tcggcatcgc tccatttccc catggccata
     2761 aatgtgtacc caaggcccag caaagcccga ttcctatcga aggaaccatt gacggaatgg
     2821 gccatcgctt gctcgtaaaa cgcttgagcc tcttggaacg cgccttgttc aacatacaaa
     2881 tccgcgatcc gccattctgc tctccgggca tttgacgatt gtggatggtc tcgaaggagt
     2941 tttttatatt ccgcaatcgc ctcggatcgg gtttgccctg agacatcatc acgcaacaaa
     3001 agctcgacca ggaaggcttt tgccgacggg acgagcgggc tttcaggata ttcctttacg
     3061 attttcccaa agaagcgttg cgcttccggc catgcgttct tcctgtatgc ggaccggcct
     3121 tcctgccaag ccagtccgtc aatccccgaa gattggcctt cctgacgagg cccgtcatca
     3181 ggcaagagtt gcacgggtcg ttcaaccggt actggattcc gtatcgaggt cgttcgttcg
     3241 gcctgtgttg tgattgaagt attgggagaa ggatgcgcag ggacctcccc gatggccggc
     3301 gaggagaacc atatccaaac ggcgatcgag aataccaatc gacgatatcg tagtcggcat
     3361 ggatacacgg cgatagggca tcagcaaagc tcatgcccgt ataccggtag gaaattcgct
     3421 ggaaattagg cgagttatcc agaaaaatca cgagcctgga tggattgtgt caggattcgc
     3481 atcggctagg tcgccaggtc gtcaatcttt tgactggaag gccgcggcca gagcgggaga
     3541 gtttccagat gagatcgtgg atccacaccc ttacgcttca gtttttcaac caaggtcgtc
     3601 cggttcagat ggagcaattg cgccgcccgc gacgtgacgc cgttggcttt gcgcaaggcc
     3661 tccatgatga gatgtttttc gtactgctcc acctctctgg aaagattgat gccgtcctcg
     3721 gtgaaccgga tgaactgctc cttgagttcg gagcccgacg gcttccgaca gattttctcg
     3781 ggtagatcac cgacggaaag aactccgcgc ttcttgagca cgaccaaccg ctcgatcata
     3841 ttctccagtt ctcgaatgtt cccgggccag tcgtactcgg tcaatagatg gagggcgtcg
     3901 ggagcaaggc ctgaaacctc ggtgtgcttg gtttgattga agcgagtcag aaaatggtcg
     3961 atcaagagcg gaatgtcgct ccggcgttct cg
//
LOCUS       DATNDD010000213         3670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2066727, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000213 DATNDD010000000
VERSION     DATNDD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066727"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..852
                     /locus_tag="VN647_05170"
     CDS             238..852
                     /locus_tag="VN647_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HWG96456.1"
                     /translation="MIKAFWKTCLAGLFVLVPAWATFLILSTLFTTLDGVVGRYMSNP
                     IPGLGLLLLIVLFVVAGLLCDHVIGQGLLVRLEHRIEQIPLVQSIYLTLKGMTDVLNF
                     RSRFGRSKVVVFPFPRDGCWALGFVMGAAPPSLQVEPSRILFMVFVPTAIHPFTGFLA
                     FIPEHALRPINLPVEDAMKMEFSAGFYRPPNGWLTAPSLPPRDP"
     gene            849..1349
                     /locus_tag="VN647_05175"
     CDS             849..1349
                     /locus_tag="VN647_05175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWG96457.1"
                     /translation="MMTERIHIRPARQDDVGTIVMFNAALALETESRQLDLATLRNGT
                     LAFLDSPGYGFYIVAELPEGKNYSPVGQLMITYEWSDWRNGVFWWIQSVYVVPERRGL
                     GVFRAMHDHIFLKAKADPRVCGIRLYVERENRKAQAVYHRVGLNPSAYTVYERDFVLA
                     PSTNSP"
     gene            1369..1749
                     /locus_tag="VN647_05180"
     CDS             1369..1749
                     /locus_tag="VN647_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96458.1"
                     /translation="MKSIILGMLILNLLINTGCAFIRGNYGEELGQGDIATIQKGIST
                     RADVAAVLGAPDRIIEASGREIFHYFHYDVKSGSVFIFSRTNIKSDDIFVIFNEAGVV
                     EELVSGKRKGPLEFQFWPFDWPFH"
     gene            2286..3140
                     /locus_tag="VN647_05185"
     CDS             2286..3140
                     /locus_tag="VN647_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWG96459.1"
                     /translation="MDEETSDHPSHHYGNVRSSSYIPADQDIEFLQRDELRPIRIGLE
                     LLKPELIQKEEHIRSTIVVFGSARLREPAAVKQALRLAEEQAAQAPDDRIRRQRVAIV
                     KGQLELATYYDVAREFGRLVSSTCQIDGRCDYVIVTGGGPGIMEAANRGAADVSAKSI
                     GLNITLPHEQHPNPYITPRLSFQFRYFAVRKMHFLIRAKALVAFPGGFGTIDELFETL
                     TLLQTGKIESLIVVLIGRDFWERLVNWQLLVEYGLIMQTDLHLFHYAETAQEAWDLIA
                     RHNGVPTA"
     gene            3137..>3670
                     /locus_tag="VN647_05190"
     CDS             3137..>3670
                     /locus_tag="VN647_05190"
                     /inference="COORDINATES: protein motif:HMM:NF027971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWG96460.1"
                     /translation="MKLSFHGAARSVTGSRHMLEVPGFRLLFDCGMFQGRREEAVRRN
                     RDLGFESKSIGAVLLSHAHVDHSGALPVLPQQGFSGKVYLTRASADLTSIMLEDSARV
                     QENDCRYVNKQERRRGKICIRPFYDGDDVRKIVRRFEGERYGAQLKIAPRITASFHDA
                     GHILGSAAVRVKYTARGI"
BASE COUNT          822 a          984 c          962 g          902 t
ORIGIN      
        1 agcggcattg agcttttgaa tgagagactc ctcagccaga ctcaccgcct gtttggtctc
       61 tggacaacaa aggatcgcca ggagctcctt atcaatactc atgaccgttg acctcctgct
      121 gcattgtggc aggtagaata atcgagagag ctatgaagcg caagctgtca taaggaattc
      181 ctcatgctcc gtcatttctg ctacactcga catgctttct gcaaggaaaa tctcgtcatg
      241 atcaaagcat tttggaagac ttgcctagca gggctctttg tcttggtgcc ggcctgggcc
      301 acgttcttga ttctctcgac tctattcacc acgcttgatg gggtcgttgg ccgatacatg
      361 tcgaatccaa tccccggcct cggtctgcta ctgttgatcg tgctgttcgt cgtcgccggt
      421 ctcctttgcg atcatgtaat aggacaaggc ttgctggtaa gactggaaca ccgcattgaa
      481 cagatcccgc tcgtgcagag catctatctc acattgaaag ggatgaccga tgttttaaac
      541 ttccggtctc gttttggccg cagtaaagtg gtggtcttcc cctttccgcg ggatggatgc
      601 tgggccttgg gttttgtgat gggggcggcg cctccatccc ttcaggtcga gccctcccgc
      661 attctcttca tggttttcgt cccgaccgcg attcacccct ttacaggctt cctcgccttc
      721 atcccggaac atgcgctgcg tccgatcaat cttcctgttg aggacgcgat gaaaatggag
      781 ttttcggcag gattctatag acctccaaat ggatggctca ccgccccatc gctccctcct
      841 cgtgatccat gatgactgag cgcatccaca tcagaccggc ccgacaggac gatgtgggta
      901 ccatcgttat gttcaatgcc gccctggcgc tcgaaacaga gagtcgccag ctcgatcttg
      961 ccacattgcg caatgggacc cttgcattct tagacagccc cgggtatgga ttttatatcg
     1021 ttgccgaact tccagaaggc aaaaactaca gcccggtcgg ccagctcatg attacgtatg
     1081 aatggagcga ctggcggaac ggagtctttt ggtggatcca gagtgtctat gtcgtacccg
     1141 agcggcgcgg tctgggggta tttcgagcca tgcacgacca tatcttctta aaagccaagg
     1201 cagatccacg ggtttgcggg atcagattgt atgtggaacg ggagaatcgc aaggctcaag
     1261 ccgtgtacca ccgtgtgggg ctcaatccct cagcttatac cgtctatgaa cgagattttg
     1321 ttctcgctcc gtctacaaat tcaccatgag agcactagag gtggcagcat gaagtccatc
     1381 atcctaggaa tgctgattct aaatcttctg atcaatactg gatgtgcctt tatccgagga
     1441 aactatggcg aagagttagg tcaaggtgac atcgcaacca ttcaaaaagg cattagcaca
     1501 agagcagacg ttgctgctgt gttgggcgcc ccagaccgta tcatcgaagc aagtggtcga
     1561 gaaatctttc attactttca ctatgatgtg aaatcggggt ctgtgttcat cttctcgcgt
     1621 accaacatca aaagtgatga catctttgtt atcttcaatg aagcaggagt agtagaagaa
     1681 cttgtatctg gaaaacgaaa aggtcccctg gagtttcagt tctggccctt cgactggccc
     1741 ttccattaat tccttataac gcttcatgat gctgcgaaat cggttcattt gtctgatcct
     1801 agcactaacg ctcacgtcac tgaccggatg ctcacaaggt cccgtgacat ggcggcgagt
     1861 aacgcttaat cagactattt ctaccgaaga tgtgatcttt atagtacctg gcaagactga
     1921 tatttcagcg gtcgttgaga gactaggtgc accaaacgat atactctctt ctaaaaatga
     1981 aatcgtgacc cgttactatt ttacggatgg taagtatttc aaagcgaatt atggctgggg
     2041 atttcgcttt cttatacccg tctttgcgcc tgacctcgat ttgggaggtg gcggaatagg
     2101 tagggatgtc ttccaagtca cttatgacga ccactggctc gttcgtgatc atgcgtttgc
     2161 ttttcactca cgctcgtccg agttcttagc ttggccattc agggactgaa ccattctgct
     2221 taccaatagg taggcttacc tcggtgaatt gccggtttcg gtcagccccg ctataatggc
     2281 gagccatgga tgaggaaaca tccgaccatc cgtcccatca ctacggtaac gttcgctcct
     2341 cttcctatat cccagccgat caggacatcg agtttctcca acgagacgaa cttcgcccca
     2401 ttcgcatcgg actcgaactg ctgaagccag aactgatcca aaaagaagaa catatcaggt
     2461 ccaccatcgt cgtcttcggc agcgccaggc tccgggaacc ggcggctgtc aagcaggcgc
     2521 tccgtctggc ggaagagcaa gcagctcagg ccccggacga tcgcatccgc cggcaacggg
     2581 tcgccatcgt caaagggcag ctcgagttgg caacgtatta tgatgtggcg cgagaattcg
     2641 gtcggttggt gtcgtccacg tgccaaattg acgggagatg cgactacgtc attgttaccg
     2701 gaggggggcc cggtattatg gaagccgcca accgcggggc agcggacgtg agcgccaaat
     2761 caatcggact caacattact ctgccgcatg aacagcaccc gaacccatac atcacgccga
     2821 ggctgagttt tcaatttcgc tatttcgccg tcagaaaaat gcatttcctc atccgagcca
     2881 aggctctcgt ggcgtttccc ggggggttcg gcacgatcga tgagttattc gaaacgctta
     2941 ccctgctcca gaccgggaaa atcgagagcc tgattgtcgt cttgatcgga cgagactttt
     3001 gggagcgctt ggtcaactgg caactgctcg ttgagtacgg cttgatcatg cagacagatc
     3061 ttcacctgtt tcactacgcc gagacggctc aggaggcttg ggacctgatc gcacgccaca
     3121 atggagtacc caccgcatga aactctcatt ccatggtgcc gctcgttcgg tcacaggcag
     3181 tcggcacatg ctggaagtac caggcttccg gctgctgttc gattgcggaa tgtttcaggg
     3241 gcgccgagaa gaagcggtcc ggcgaaaccg cgacttgggg tttgagtcaa aatccattgg
     3301 cgccgtcttg ctctcgcacg cccatgtcga ccattcggga gccctcccgg ttttgccgca
     3361 gcaggggttt tccggaaaag tctacctcac cagagcatcg gccgatctca cgagcatcat
     3421 gctcgaagac tcagctcgtg tgcaagaaaa cgactgtcgg tatgtcaaca aacaggagcg
     3481 cagacgcggg aagatctgca ttcgcccgtt ttacgatggg gacgacgtgc ggaagatcgt
     3541 ccgcaggttt gagggagaac ggtatggcgc tcagcttaag atcgcgcctc ggatcaccgc
     3601 atcgtttcac gacgccggcc atatcttggg atcggctgct gtccgagtga aatatacggc
     3661 gcgcggcatt
//
LOCUS       DATNDD010000214         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4039087, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000214 DATNDD010000000
VERSION     DATNDD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039087"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1523)
                     /locus_tag="VN647_05195"
     CDS             complement(228..1523)
                     /locus_tag="VN647_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWG96461.1"
                     /translation="MRLGRPLSVIICLWTALAACQMLPGNWRPGWNEPWDDGKPVMET
                     AGSWKLTEAETQDGRFLALAVSGGGSRAANFGAAVMLELQQRGLLEQADVISGVSGGT
                     LPAVYYGLGAKAGPFTAPALREALGYDFQSSWIRRWFLPQNIFRYWLADFTRSDIMVQ
                     VFNNRLYREATFADLKLHPKILLNATVHNDHTRFTFTDDRFALLRSGLAQYRVANAVN
                     ASSAFPGAFQDVTLQWYMDRPHYVHLYDGGPIDNLGIQAVVEYLNRNILGTTLDRLFP
                     KGCLIIAIDAAPASEHPDLNARISSRQFADYFIDTNALDATDAMLMEARRTLLARMEI
                     ATEQQDKQVRGRLPVNDLHQCSCQVLHLSLRHLLYGDDSEETRQLAERVTEIKTKFWI
                     GKDEQDALYRAADVIMKEVDAQHLLSDESLKPQCAASKQ"
     gene            1693..2385
                     /locus_tag="VN647_05200"
     CDS             1693..2385
                     /locus_tag="VN647_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG96462.1"
                     /translation="MPGIALVTGANRGIGLEVVRQLAAKQWRVFLSGRSLDSIERAAV
                     SLSAPSVTPIVLDVTDQESIDRAVKTIAQTVDHLDVLVNNAGILDDHDDSVFKIPADR
                     LRRMFETNTIGPLLVTQAFVPLLRKSAAARIINVSSRVGRLTGMGSAVPAYGISKTAL
                     NGVTCKFAASLKSSGIAVNSVDPGWVRTEMGGIDAPRSVEQGADTIVWLATEAPQSMT
                     GLFLRDRKPIPW"
     gene            complement(2447..2623)
                     /locus_tag="VN647_05205"
     CDS             complement(2447..2623)
                     /locus_tag="VN647_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96463.1"
                     /translation="MSSTGSMFIGIVGALMFLISVFAFSQRNWKGGFLWLLVGAGLIA
                     LLTYMDKPSANLLR"
BASE COUNT          710 a          887 c          904 g          653 t
ORIGIN      
        1 atacactgaa gggtaccggg gaatttacga ttcatccgga taccggaatc attgtcttcc
       61 tcaggttacc ggaacacgaa gcctatgcag cccgctttac tccgcgtgac accaagcgtg
      121 gcgccgcaca aacaggcgga caccaccatc cgcagaagca ttgatcaatg ctcatctgga
      181 gagaacatcc agagacgtgg gagggcagaa agataatgat ttgtggatca ctgtttgctc
      241 gccgcacatt ggggcttcag tgattcgtct gaaaggagat gctgcgcatc cacttccttc
      301 atgatgacat cggccgctcg atagagcgcg tcctgctcgt ctttcccgat ccaaaacttc
      361 gtcttgatct cggtcactcg ctcggccaat tgtctcgtct cctccgaatc gtcgccgtag
      421 agcagatgcc gaagagaaag atgaagcacc tgacagctgc attgatgcaa atcgtttacc
      481 ggcagccggc cccgtacctg cttatcttgc tgttcagtcg caatttccat gcgcgcgagg
      541 agggttctgc gcgcttccat caacatggca tccgtcgcat cgagcgcgtt cgtgtcgatg
      601 aaatagtccg cgaattgccg actggaaatt ctggcattga ggtccggatg ctcgcttgcg
      661 ggcgccgcgt cgatcgcgat gatcaggcag cctttgggaa agagacgatc gagcgtcgtc
      721 cccaggatgt tgcggttcaa atactcgacg acggcctgaa tgcccagatt gtcgatggga
      781 ccgccgtcgt agagatgcac gtaatgcggc cggtccatgt accactgaag cgtcacatcc
      841 tgaaaggcgc cgggaaaagc ggaagatgcg ttgaccgcat tcgccacccg gtattgcgcc
      901 agaccggaac gcaacaacgc aaaacgatcg tcggtaaagg tgaagcgagt atgatcattg
      961 tgaacggtcg cgttgagcag gatcttcggg tgcagcttca gatcggcgaa agtcgcctcg
     1021 cggtacagtc ggttgttgaa cacctgaacc atgatatcgg aacgggtgaa gtcggccagc
     1081 caataacgga agatgttctg gggcagaaac cagcggcgga tccaactgct ctgaaagtca
     1141 tagccgagcg cctcgcgcaa ggccggggcg gtgaagggac cggccttggc ccccaaccca
     1201 taatagacgg cgggcaatgt gccacccgag acaccgctga tgacatccgc ttgttccagc
     1261 agaccacgtt gttggagctc cagcatcacc gccgcgccga agttagccgc ccggctgccg
     1321 ccgccggaaa ctgcgagggc caggaagcga ccatcttgcg tttcagcctc ggtcagcttc
     1381 caagatccgg ccgtctccat gaccggcttt ccgtcgtccc aaggctcgtt ccatcccggt
     1441 cgccagttgc ccggcaacat ctgacaagct gcgagggccg tccagagaca aattatgacg
     1501 gataacggtc gacctaaccg catatcaggt ccaatggctg gctagaaaga tcatgagcat
     1561 gagtaatttc aaggggccgc gtgattgtag gacgccgcag aaagacaggc aaggtctgag
     1621 cagggtggac gagggtctcc ataggtgcat ggaattgcta taagtaagct attcaatagg
     1681 tggaggcagc gtatgcccgg cattgccctg gtcacaggtg ccaatagagg catcggcctt
     1741 gaggtagtcc gacaactggc ggcaaagcag tggcgggtgt tcctgagcgg acgatccctc
     1801 gattctattg aacgagccgc tgtctccctc tccgctccgt ccgtcactcc catcgtcctc
     1861 gatgttaccg atcaagaaag tatcgaccgg gctgtgaaga ccatcgccca gactgtcgat
     1921 catctcgatg tcctcgtgaa caatgccggg atcttggacg accatgatga ttcggtcttc
     1981 aagatacctg ccgatcggct ccggcgaatg tttgagacca acaccatcgg tcccttactc
     2041 gtcacccagg cttttgtccc cctcctccgc aagagtgccg ccgctcgaat catcaacgtc
     2101 tcgagtagag tcggacggtt gaccggcatg ggttcagcag ttccagccta cggcatttcc
     2161 aaaacggcgc tcaacggtgt gacctgcaaa ttcgcggcat cgctcaagag ctcgggcata
     2221 gcggtaaatt ccgtcgaccc cggctgggta cggactgaga tggggggaat cgatgcccca
     2281 cgctcagtcg aacagggtgc ggatacgatc gtttggctgg ctaccgaggc gcctcagtcg
     2341 atgaccggcc tgtttctccg agatcgtaaa ccgattccat ggtaaggcat tcctccagat
     2401 tttgagaacc tcgtttagga tcaagacgga tgatcatttg atcggattac cgcaataaat
     2461 tcgccgacgg cttgtccatg taggtcaaga gtgcgatgag ccccgcaccg accagcagcc
     2521 ataagaagcc gcctttccaa ttcctctggc tgaatgcgaa gacactgatc aggaacatca
     2581 gcgcccccac aataccgatg aacattgaac ccgtcgaact catcaggccc tccatagaag
     2641 agattcctgt ttagcatgtc gagaggaacg aactctaggg cttgcgcgtg atttacaagg
     2701 ttgaggacca gcgcgttccg gtggtagtca tagatgtgac cgcgcaatct gctccctaac
     2761 ttctgctcag cgctgttcgg gctctttgtc ttcgagcagc aagcgcaagg ctctactgct
     2821 ggccaaacag ccgttcaaga agcgcctcgc ggcttatctc aaaatgcgcg gccacatcgg
     2881 cgagaatggc cgagaaggtg ccgatgcgaa gcggggtatg ctgtggggga ttgtgagatg
     2941 atgctcgcca tgctcgtggg tggtgagtcg aaggtgaccg ccggcttggc gggtaatcga
     3001 gtagccgagt ttgcgaaggg cctgagcgag atcgctcccg gacagatcgc ggggaagcct
     3061 catacgacga taacttcctc gcggacatgg tgaaggcgga cgatctttgg ggccatgcct
     3121 tcttcaaagt gacaacgaac tgcctcacgg actt
//
LOCUS       DATNDD010000215         2671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2666043, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000215 DATNDD010000000
VERSION     DATNDD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666043"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..653
                     /locus_tag="VN647_05210"
     CDS             360..653
                     /locus_tag="VN647_05210"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWG96464.1"
                     /translation="MVRRIVERFHPERIILFGSHARGTAGPHSDVDLLVVMQPQGSKR
                     RRAVEIHGLLAGMGVPKDIIVVTPEEFEAYRDAPGTVIRTAWQEGKTLHDRAA"
     gene            complement(1112..2206)
                     /locus_tag="VN647_05215"
     CDS             complement(1112..2206)
                     /locus_tag="VN647_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWG96465.1"
                     /translation="MLEPTMNPSLLNVASETAAASVPPVDFLKHWTTDCREVKTFRGH
                     SHGIWAVTFSPDGSMLASGGAERLVRMWDIETGRLLRSLRGHTNDIRAIVFTPDGQAL
                     VTGSEDRTIRLWNGKTGEPMKLLFSRYDHNVCSLSLSPDGLMLARGSHNKDIKIWEVT
                     TGTELMTLLGKDEFDHHWSVCVAFSPDGIHLASGSDIGKIRIWEVLPSGEEKVLHNGH
                     WREDQEDTTETRGYFIADDGGFQKPMDYWIGAMTFTPDAKLLITGSRDATIKLFEMPN
                     AVEKKTLTGHKDWVRCLAVSPDGKVLVSAGDDQTIKFWDLATGRNFRTVKGHSGPVRC
                     VTFSPDGKRLASASWDRTVKLWEGGAKTEE"
     gene            2394..>2671
                     /locus_tag="VN647_05220"
     CDS             2394..>2671
                     /locus_tag="VN647_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96466.1"
                     /translation="MEIRFQPALLQEVIDSFVEKTEREGDPTYYKEFHEHADPIYEKF
                     ILEDREGEFKKLYQYLFGIWGFSDIVRDSFHEYSLLKEKVGIVLVKGVL"
BASE COUNT          567 a          710 c          761 g          633 t
ORIGIN      
        1 gggttgcggc ggtgaatgca gacggaacct atgggaagtg gcggtacgcc gtcgcaaaga
       61 aagtgtcaga cgtttctggc ctgcttcaat aagcagtgct cttactcttc agtcttctct
      121 ccccccttcc cacagcttca ccgtccgatc ccatgacgcg ctggcgaggc gtttgccgtc
      181 gggggaaaag accagaccac ggaccccgcc gctccgactc tgagggccga agcgggtgtg
      241 aatagctcct tgtagatgat ttctgaaggc taaaggagta ttctccggct caagagttca
      301 agaataagat ggtccaatca gtaaggagtg caagcacagt tcaagctatt atcgttgaaa
      361 tggtgcggcg tattgtcgag cggttccatc ctgaacgaat cattctcttt ggatcacatg
      421 cccgcgggac ggctggtccg catagcgatg ttgatttgct tgtcgtgatg cagccacagg
      481 gttctaaacg gaggcgagcg gttgagatcc atggattgtt agccggcatg ggcgttccta
      541 aagatatcat tgtcgtgact cctgaagaat tcgaagcgta ccgagacgcg ccagggacgg
      601 tgataagaac ggcgtggcaa gaaggaaaga ctctgcatga ccgtgcggcc taaggatgag
      661 gatcttgtcc gggcttgggt cctaaaggcc gatcatgact ggctcaacat cgaaaataat
      721 cttgccgcgc gagatgtccc ttgggatacc gttggttttc atgcacagca atgcgtagag
      781 aaatacctca aagcccctct cgtttctcgg caagtcgacc ctccgaagat tcacgatctg
      841 actgaacttt atgcactatt gccgaatggc ctgctggtag actttgacgt tcgcttgctc
      901 gaagaattga atccttactc gattgaagga cgataccctg gagtttggga gccggttgaa
      961 cacaagcaga ggcatttcgg gctgttgagg ctgcgagaac gattcgcgag gcgatccgcc
     1021 gagtcctccc ttcagcctgt attgtctgac tatacagatc agaagagata ttctagtggc
     1081 agccggtttt gctgcgggct gttctctcct gctactcttc cgtcttcgct cccccttccc
     1141 acagcttcac ggtccggtcc caggacgcgc tggcgaggcg tttgccgtct ggggaaaagg
     1201 tgacgcagcg cacggggccg ctatggcctt ttacggtccg gaagtttcgt ccggtggcca
     1261 gatcccaaaa cttgatcgtc tgatcgtcac cggcgctgac caacactttt ccgtccgggg
     1321 acacggcgag acagcggacc cagtctttgt ggccggtgag agtttttttc tcgaccgcgt
     1381 tcggcatctc gaacagcttg atggtggcgt ctcggctgcc ggtaatcaaa agtttggcat
     1441 ccggggtgaa cgtcatggcg ccgatccaat agtccatcgg tttttggaat ccgccgtcat
     1501 ccgcgataaa gtagccgcgg gtttcagtgg tatcttcctg gtcctccctc caatggccgt
     1561 tgtgcaacac cttctcctcg ccgctcggga gcacttccca aatcctgatc ttcccgatgt
     1621 cgctcccgct ggcgagatga atgccgtccg gtgaaaacgc cacgcagacc gaccagtggt
     1681 ggtcgaattc atcctttccg agcaatgtca tcagctcagt gccggtggtc acttcccaaa
     1741 tcttgatgtc tttgttgtgg ctcccgcgcg cgagcatgag gccgtccggt gagagcgaga
     1801 ggctgcagac attatgatcg taacgtgaga acaacagttt catcggctcg ccggtcttgc
     1861 cgttccacaa tcgaatcgtg cggtcctcgc tgccggtgac gagggcctgt ccgtccggag
     1921 tgaagacgat ggcgcgaata tcattggtgt ggccgcgcaa agaacgcagc aaccgaccgg
     1981 tttcgatatc ccacatgcgt acgaggcgtt cggcgccgcc gctggcgagc attgatccat
     2041 caggggaaaa ggtgaccgcc cagattccat gcgaatggcc acggaacgtt ttcacttccc
     2101 ggcagtcggt ggtccagtgt tttaagaaat cgacgggcgg taccgatgca gctgccgtct
     2161 cagaagcgac gttcagaagc gaagggttca ttgtgggttc caacatgttg gtttgcgttt
     2221 ctcggccgct tcctccttga ttgagcaaca gccgggccag tataattctg tcggtcgctc
     2281 ggatcgtaag agatgactcg cggccaagtc aagctgactc catcaccatg tcgtcgatgg
     2341 gttatcgtat aggatatctc aaggtggcct catcaagccg ctgaggttct tccatggaaa
     2401 tcagattcca accggctttg ctccaggaag tgatcgactc gttcgttgag aagacagaac
     2461 gggaaggcga tccgacctac tacaaggaat tccacgagca cgccgacccg atttatgaga
     2521 agttcatcct cgaagaccga gagggggagt tcaagaaact ctatcaatat ctcttcggta
     2581 tctggggatt ttcagacatt gtgcgggatt cgtttcacga atattcgctg ttaaaggaaa
     2641 aggtcggcat cgtcctggtc aagggcgtgc t
//
LOCUS       DATNDD010000216         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2615092, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000216 DATNDD010000000
VERSION     DATNDD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615092"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VN647_05225"
     CDS             <1..429
                     /locus_tag="VN647_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HWG96467.1"
                     /translation="QRHMSVGEQEDSVQTTPYQEAGEFAGITRLVDEFYINMDTLPEA
                     ETIRSMHPPDLTESRKKLTYFLCGWLGGPKLFQQHYGPISIPGFHKQFPIGYEERDAW
                     LLCMQRALAVQPYSDQFKDYLLAALSIPAERVREVNAGEF"
     gene            1008..1148
                     /locus_tag="VN647_05230"
     CDS             1008..1148
                     /locus_tag="VN647_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96468.1"
                     /translation="MAWPSQAMQAAETGIVTRQLRAHLAPTVALTATDINLGMFDYAR
                     GN"
     gene            complement(1370..2626)
                     /locus_tag="VN647_05235"
     CDS             complement(1370..2626)
                     /locus_tag="VN647_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWG96469.1"
                     /translation="MKKVSGRYTAVLAFLLCLLYLGYRIYDNRSYAAMLREATLKAIA
                     PPVTIISPKPVSATESITLPGTIQGWYEATTYARVTGYVKMWHKKYGDWVNKGDLLAE
                     ISTPDLDAEYRQAIKDLESQRAKYRLAEVTAQRWRALRPNHAVSEQAITVKEQELRAQ
                     AAAVEAAHHQVMNVEAFIGFKKILAPFDGVVVQRNINVGDLISKQGTLHTPNAKTNLY
                     TVAVIDRLRLFIKVPAYFGPFLRPSLTADVTVPQLPNRHWTAKFLTVSRGFDVGTRTA
                     TSVFTIDNADHALWPGSYAIVHLTAPVDRPAVVIPTSALVYEKDDALQAAVLTEDNRV
                     HLKPITVSNLMDSAVEVSAGLSVSDRLIDNPPHTLLEGDQVRIVTPRPGYLITTEEPE
                     QRLHQNGDHGGLTALSTPEPTKRQRP"
BASE COUNT          593 a          754 c          771 g          575 t
ORIGIN      
        1 caaagacata tgtccgttgg tgaacaggaa gacagcgtgc aaaccactcc gtatcaagaa
       61 gcgggtgaat ttgcgggaat cacccgcttg gtggacgagt tttacatcaa tatggatacg
      121 ctgcctgaag ctgaaaccat cagaagcatg catcccccgg atctaaccga atcacgcaag
      181 aagctgacct atttcctgtg cggctggctg ggaggcccta agctgtttca gcagcactat
      241 gggccgatca gcattccggg attccacaaa caatttccca tcgggtatga agagcgcgat
      301 gcctggctgc tctgcatgca acgagcgctc gccgtccaac cttatagcga ccaattcaaa
      361 gactaccttt tagccgccct cagtatccca gcggaacgag tcagagaagt gaacgcagga
      421 gaattctagt atctctcccc gtctcctgca caggctgatc tgttcccatt ggagtacacc
      481 ccgcacgttg ggatacacct ctggatcctt caggatacag aacctcgatc ttcataagat
      541 cctcttgagg aaccgttatc cttcaaaatc aatgcgatga acgaaaaact cgcccaactc
      601 agttcaatca actgtgtggc attctccttg cgtcatccaa aacgagaaag agtggcggcg
      661 gccagaaatt catgaaggac aagatggctt tcctcttcaa tcattccagc gagcagcaac
      721 tctcctgctc actcaaggcg gaatcaatct cgatcggatg tgaagcatgg atcgacgtgg
      781 gccggttttc aacgagaaaa cagtccgtgc agcagatcgc tggggacccc ggagcctctg
      841 aagaccaaga cccctcaggt tcaactcttg tccacggttc attgacgaaa tcattcgcca
      901 tcctgtgtcg gaagcttcgg cgaggttccg ccaaaggcct agactaacta cgaccgtaaa
      961 atcatccggt tcatcggcag aacgacgcgt caagcagttt caaaggcgtg gcctggccat
     1021 ctcaggccat gcaagccgcc gagaccggta ttgttactcg gcagctgcgt gctcacctgg
     1081 cgccgactgt ggcgcttact gcaaccgaca tcaatctcgg catgttcgac tatgccagag
     1141 gaaactgacg gacctgaagg gaattgagtt gacggtgaat ggctttgacc gcatcgagat
     1201 tttccccgtt ccgaaggagt gctccagcga cacggctaaa tcactggcga ttcaacgatg
     1261 caggcgattg tggtaacggc acgagcgaag gatgacgtga acctataagc atgaatgttc
     1321 atggcccgaa gggccgactg ccggcagtga gcgatcaccg ccgaacacat caaggccgct
     1381 gtcgcttcgt cggctccggc gtactgagtg ccgtcagtcc gccatggtcg ccgttctgat
     1441 ggagtcgttg ctcaggctct tctgtcgtga tgaggtaccc cggtctcggc gtgacgatgc
     1501 gtacctggtc gccttcgagc aacgtgtggg ggggattatc gatgaggcgg tcgctcacgg
     1561 aaagtcccgc tgacacctcg accgcgctgt ccatgagatt gctcacggtg atcggcttca
     1621 agtgcacgcg gttgtcctcc gtcagcacgg ccgcttgtaa ggcatcatcc ttttcataga
     1681 ccagggcgct ggtcgggatg acaacggccg gccgatcaac tggagccgtg aggtggacga
     1741 tggcgtagga accgggccag agcgcgtggt ctgcattgtc gatcgtgaag accgaagtcg
     1801 ccgtccgcgt cccgacgtcg aatccacggg agaccgtgag gaacttggcg gtccaatgac
     1861 gattgggcaa ttgcggcacc gtcacgtcgg ccgtcaagct tgggcgaagg aagggcccga
     1921 agtacgccgg caccttgata aagagacgca accgatcgat gacggccacg gtatagaggt
     1981 tggttttcgc attgggggtg tggagagtcc cctgcttact gatcaaatca ccgacgttga
     2041 tgttacgctg gacgaccacg ccgtcaaaag gggcgaggat ctttttgaac ccaatgaacg
     2101 cctcgacgtt catgacctgg tgatgcgccg cctcgaccgc cgccgcctgg gctctcagtt
     2161 cttgttcctt caccgtgatt gcctgctcag agacggcatg attggggcgg agggcacgcc
     2221 aacgctgcgc ggtcacctcg gcgagccgat acttggcgcg ttgcgattcc agatccttga
     2281 tggcctgccg atattcggcg tcgaggtccg gcgtgctgat ttcagcgagg aggtcgcctt
     2341 tgttcaccca gtctccgtat ttcttgtgcc acatcttcac atagcccgtg acccgggcgt
     2401 aggtcgtcgc ttcataccag ccttgaatcg tgccgggaag cgtgatgctc tcggtcgcgg
     2461 agaccggctt gggagagatg atggttacgg ggggagcgat ggctttgagc gtcgcctcgc
     2521 ggagcatcgc ggcgtagctt ctgttgtcat aaatccgata gccgagatag aggaggcaaa
     2581 gcaggaacgc taaaaccgcc gtgtatcttc cactgacttt cttcatcgtg cggcgagtcc
     2641 ttttggctca gccgttcgcc ggctatagac aatcgcatag acacagggca cga
//
LOCUS       DATNDD010000217         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3978562, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000217 DATNDD010000000
VERSION     DATNDD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3978562"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VN647_05240"
     CDS             complement(<1..606)
                     /locus_tag="VN647_05240"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWG96470.1"
                     /translation="MKILIGLLVVVVLLVGVVVALPFLIDLNKYQDQYKPLIEDALNR
                     KVQLQDIRLTIWPRIGARVAGFAVLDDSSFGSTPFASLSSLDVGVKLMPLLSGKVEVE
                     DITLHQPVITVIKNKNGVLNASTIGRKGVAVPEKPSRAPIPSTEGPLKILALLAVDRV
                     SIDGGKLTYRDLSAAKPTEYVLQDMELLLQDVRLGRTPRLHF"
     gene            complement(815..1033)
                     /locus_tag="VN647_05245"
     CDS             complement(815..1033)
                     /locus_tag="VN647_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96471.1"
                     /translation="MRKKTNIMSDNQTVRSRREFKPKDGKVENQGRVIRPMKQTWQLF
                     TDSLTMFTDDFMEDRQQPSHQKRKSSFA"
     gene            complement(1439..>2597)
                     /locus_tag="VN647_05250"
     CDS             complement(1439..>2597)
                     /locus_tag="VN647_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HWG96472.1"
                     /translation="GAIVAKKRTYQGKVPLHDKYGPEAKYAVEAEALLPTTKYEEEVA
                     RGLELGLQGADSIKDRRIPTFSRGELPHFAGINTFIKAPYVEDVRKCGQYDVAILGAP
                     FDGGTTYRSGTRFGPQGIRKISALYGTYSFELGVDLRESVSICDVGDVFTIPGNIEKT
                     FDQVSKGVGHVYASGAFPVVLGGDHSLGFATVRGVAQNMNGKKLGILHFDRHVDTQET
                     DLDERMHTTPWFHATNIPNVPAKNLVQIGIGGWQAPRPGVKVGRERQTTIMTVTDCVE
                     MGIENAAKQALEVAFDGVDAVWLSFDVDCLDAAFVPGTGWPEPGGFLPREVLKFLQII
                     ADTKPLAGMEIVECSPPYDAAEITSLMATRVICDVLACQVRSGNLLNRKKR"
BASE COUNT          551 a          751 c          710 g          585 t
ORIGIN      
        1 gaaatgtaga cgtggagttc ggccgagccg aacgtcctga aggagcaact ccatatcctg
       61 caacacatac tccgtcggct tggcagccga cagatcgcga tatgtcaatt tgccaccgtc
      121 gatcgacact cggtccacgg ccaagagcgc gagaattttg agcggtccct ccgtcgaggg
      181 aatcggagcg cgcgacggct tctccggcac cgccacgcct ttgcgaccga tggtggaggc
      241 gttcaggact ccgtttttgt tcttaataac cgtgatgacc ggctggtgaa gcgtgatgtc
      301 ctccacctcg accttgccgc tcagcaacgg catcagcttc acaccgacat ccaacgagga
      361 gagcgatgca aagggagttg agccgaagga cgaatcgtcc agcaccgcga atcctgccac
      421 acgtgcaccg atcctgggcc agatcgttaa gcgaatatct tgtagctgaa ccttgcgatt
      481 gagcgcatct tcgatgagcg gcttgtactg gtcttgatac ttgttgaggt cgatcagaaa
      541 gggcagcgcc accaccactc cgacgagcaa tacgacgaca acgagcaagc ctatcaggat
      601 tttcatctcg atcgcctcca gttaaaaatg gagtatataa atggagtctc ggtgagtcaa
      661 acgaagaggg ctgcacacaa taatcgtgag ggtgctctac aatatttgac ttcgctgcaa
      721 ttcctagtga tctgtgtagc gattaaactt ctcggtatag tgggaggctg ttgtttgatg
      781 aggtggatgc agatgttggt atccagcacc accttcatgc gaaggaggac tttcgtttct
      841 ggtgtgacgg ctgctgccga tcttccataa agtcgtcggt aaacatggtc agactatcgg
      901 tgaacagctg ccaggtctgc ttcattggac gaatgactcg tccttgattc tcgactttgc
      961 catccttcgg tttaaattcc cgtcgacttc taaccgtctg attgtcactc ataatgtttg
     1021 tcttcttccg catcggcatc gtaagcctct ttctcaatga caatattcca tgcccatgat
     1081 gaagagtggt gcgtcatctg tcaacggaga ggcaaagcga ataacgagct cacgccgccc
     1141 aggcggtgtg ttgcgccgag ctcaatggac caggaaggat tcgcaccgct gcgaccgggc
     1201 tttcgactga gccgtggact ggctgaatct aacgatcgcg gatgagcgac atatgttaat
     1261 gcgcacacga ttgcaaaacc gtgcctgatc gatcgctttt ttgaggaagc cttgcagcag
     1321 gagaatccct tttcccatca acgcgcagga gcaaagctcg ccactgacga agtgcctagg
     1381 ggcttgcatg aatgcaagcc cccgagcagc tgtttcgtca tctctgaatc agagcatctc
     1441 agcgtttctt cctgttaagc aggttaccgg accgcacctg gcaagccaga acgtcgcaga
     1501 tgacccgcgt cgccatgagg ctggtaatct ccgccgcgtc gtagggcggc gagcactcga
     1561 cgatctccat tccggccagc ggcttcgtgt ccgcgatgat ttggagaaac ttcaacactt
     1621 cacgcggcag aaaccctccg ggctccggcc agccggtgcc cggcacgaag gcggcgtcca
     1681 aacaatccac gtcgaagctc aaccacaccg catccacacc atcaaacgcc acttcgagcg
     1741 cctgcttcgc ggcattctcg atgcccattt ccacgcagtc ggtcacggtc ataatggtgg
     1801 tttggcgttc acggccgacc ttcacgcccg gtctgggggc ctgccagccg ccgatgccga
     1861 tctgcacaag attcttggcc ggcacgttgg gaatattcgt cgcgtgaaac cagggcgtgg
     1921 tatgcatgcg ttcatcgaga tcggtctcct gtgtgtccac atgccggtcg aagtggagga
     1981 tgccgagctt cttgccgttc atattctgag ccactccccg cacggtggca aagcccaggg
     2041 aatggtctcc gccgagtacc acgggaaacg cgccgctggc gtagacatgc cccacgccct
     2101 tgctgacttg atcgaaggtc ttctcgatgt tgccgggaat cgtaaacacg tcaccgacat
     2161 cgcagatcga cacggactcc cgcagatcca cgccgagctc gaaactatag gttccgtaca
     2221 gtgcggagat tttgcggatt ccttggggcc cgaacctagt gccggagcgg tacgtcgttc
     2281 cgccgtcgaa cggcgcgccg agaatggcta cgtcgtattg gccgcactta cggacatctt
     2341 cgacatacgg agccttgata aaggtgttga tgccggcaaa atgcggcagt tctccccggc
     2401 tgaacgtggg gatccggcga tccttgatgg aatcggcgcc ctgcaggccc aattcaaggc
     2461 cccgggcaac ttcttcttca tatttcgtgg tcggcagcag cgcctccgct tcgaccgcgt
     2521 acttggcctc cggtccgtat ttgtcatgca gtggaacctt gccttggtat gttctcttct
     2581 tcgccacgat tgctcct
//
LOCUS       DATNDD010000218         3206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1184014, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000218 DATNDD010000000
VERSION     DATNDD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184014"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..688)
                     /locus_tag="VN647_05255"
     CDS             complement(152..688)
                     /locus_tag="VN647_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWG96473.1"
                     /translation="MRRASLLLSIALLCLVASACQHTTDVIPDRLEGRVDHDLRYADI
                     KDHPETYRGKLMLVGGKVLSAKRTQQGTELEVLQIPLSEELVPTGSDTETKGRFVIID
                     RNNDKVSDPAAFDDEKKRVTVVGEVLGTTTVRIDDVQQQVPELAVKNITIWDWDRRNS
                     RYGPYYSYAYRYGYPYFW"
     gene            1152..2093
                     /locus_tag="VN647_05260"
     CDS             1152..2093
                     /locus_tag="VN647_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96474.1"
                     /translation="MMVKLFRNGASWIGFGPVLLMATVLQAACSLDRQILHGRGDLQV
                     GLQHDPTTDVIGLGDMGSGSASPRNDHPYAFAQTELRQLLGSLFVAALDDDEQRIRHL
                     DVSLYRADELNRLVPWLISAFSRASRTDRVFFSLASTPFSSTEGRTTGTLFVRHHYLH
                     VALNPFVLPVEGETAKIVGRSIIIKAAEPVQEVFVEDSGAHLWTKSDTMHISLRIPEE
                     LPPELSVSQSAEAIETSDQGGRMEGTSARTNTETTRENSYKTLQRQVENLAAALKELQ
                     ARIAGQASELESVKAEMKRLQDQNAKGAHAPITSPLE"
     gene            2814..>3206
                     /locus_tag="VN647_05265"
     CDS             2814..>3206
                     /locus_tag="VN647_05265"
                     /inference="COORDINATES: protein motif:HMM:NF042585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6733 family protein"
                     /protein_id="HWG96475.1"
                     /translation="MNLLMSCERSFLRKTVFTTVCTLVGLLHLLTVSLATAQEAKEAQ
                     KEFDRFNVSVSLNQDKFFGFYPIIAGTYYLRQDFGIAFGSTLYGHLSNPRPDWSNPWT
                     WVYGGIHKSFMDGKLVLRPQVGFTNGQVN"
BASE COUNT          753 a          892 c          820 g          741 t
ORIGIN      
        1 actccattac accactcctt gccaatgttc gagaggaagc gattcctgca tgagcgatcc
       61 cgccagggcg tctcaataaa gccgaccgag ccatccatgc cgcagaggtg ctccacgagg
      121 atggagaggc cgatttcgcc gtaggtaggt gttaccaaaa atagggatac ccgtagcgat
      181 atgcatagct ataatacgga ccatacctgc tgttcctacg atcccaatcc cagatcgtaa
      241 tattcttgac ggccagctca ggaacttgct gctggacatc gtcgattcga acagtagtcg
      301 tgcccagcac ttctcccacg acggtcacac gtttcttctc gtcatcaaac gccgccggat
      361 ccgatacctt atcgttgttg cgatcgatga tgacgaaccg gcctttcgtc tcggtgtcag
      421 acccggtggg aaccaactct tcggacagtg gaatctgtag cacctcgagc tctgttccct
      481 gttgcgtccg ctttgccgac aacaccttgc cgcccaccag catcagcttt cctcgatagg
      541 tctcaggatg atccttgatg tcggcgtaac ggagatcatg atcgacacgt ccttccaagc
      601 gatcgggaat gacgtccgtc gtgtgttgac atgcgctcgc aacaagacat aacagagcaa
      661 tgctcaacaa aagacttgcg cgcctcatgg ctaccttcct ttctcttaga ctattcgacc
      721 acctgtgaaa tcgaccatgc aatatctatc ccgccaaatt ggatgctgcc acaaatgcct
      781 gcaggggtta tggtgctaga cgttgaataa cccaaaaacc gcggtcagca cgagcaacgc
      841 aagcatatcg tcatcatgga tcttcacttc ctttcacgag gatctttcca ggatgccccc
      901 gctcggaaga gtgaggagga atggccatcg ggtagcggag aacaaaccct tctggttcaa
      961 aaagccacgc ctggaagagc gaggttctta ctcaatagag ggagcatact gttcgaaaat
     1021 gaatggtcga tgatgagagt gtgaagaaat cttcatcttg atacgatggg cccatatcac
     1081 acttgaacgg cctgctattg actgaccagg atggacctga tacagcgctg gttattccag
     1141 gcagggcgac catgatggtg aagctctttc gaaacggtgc ctcgtggatc ggattcggtc
     1201 cggtcttgct gatggctacc gttcttcagg ctgcctgcag cctggatcgg cagatcctcc
     1261 atggccgggg cgatctccaa gtgggcttgc agcatgaccc caccacggac gtcatcgggc
     1321 tcggcgatat gggatccgga tcggcatctc cccgcaatga tcacccctac gcttttgccc
     1381 aaaccgagct gcgacaactc cttggatcgc ttttcgttgc cgcgctcgat gatgacgagc
     1441 agcgaatcag gcacctcgat gtttcgctct acagggcgga tgaactgaac aggctcgttc
     1501 cgtggttgat ttccgcgttc agccgcgcaa gccggacaga tcgagtgttc ttttccctcg
     1561 cctccactcc gttttcctcc accgaaggca ggaccacggg aactctattc gtgcggcacc
     1621 actatctgca cgtggccctc aatccattcg ttctcccggt cgaaggtgaa acagcgaaaa
     1681 tcgtcggacg ttcgataatc atcaaggccg ccgaaccggt tcaggaggtc ttcgtcgaag
     1741 attcgggcgc ccacctgtgg accaagtcgg acacgatgca tatttcgctg agaatcccgg
     1801 aagagcttcc acctgaactc agtgtctcgc agtcggctga agccatagag acgtccgatc
     1861 aaggggggcg catggaaggt acttctgccc ggacaaacac cgagacaacc cgagaaaaca
     1921 gctacaagac attgcagcgg caagtcgaga acctcgctgc cgccctgaaa gagttacaag
     1981 ccaggattgc cggacaagcc agtgaattgg agtcagtcaa ggcggagatg aaacgtcttc
     2041 aggaccagaa tgcgaaaggg gcgcacgcgc ccataacatc ccctctggaa tagtgacggt
     2101 tcgcccgtcg cgtattctag ggttcacaac ttctacagct cttgccagcc gactatagcc
     2161 ccttcctgaa aataatactc ctctattggt cgagggcgga ccagcgaagg gactcaaatc
     2221 cccttgacca gcgtgagatc cgctcggatg tgtcaatttg tcgcattaga gcatttgtgt
     2281 gtcaatatta cacataacta cacaacgaga gcggctgttt ttctgaaatg tggttggtgt
     2341 ttttagcacg gcttgtgcag cctgcaaatt gatcccatgg tatccaaata aggctttaac
     2401 cctgaggtgc tgcagatcta gggatctccc ctagccgcga aagatgctga agcgtagtac
     2461 attcatctgc cctgcatcgt gtggcaaaga aacgcgagag accagtcttt tccatttcgt
     2521 cttttcgaag tgacgatcag tcggggcttg gcggcactcg gctatcagct cattcggaat
     2581 acgctcgcaa tgctgcatga ttcctttgaa gaacgcctcg acttgccatc ggcatcgata
     2641 gaggtcggtg atcgtgatgc cggcaaggct cgcacccatc tcatcagagg gtcgcaaccg
     2701 tctatttacg gttcaagtag acgacagacg aaaccatgga aaacccttgg ctgcacattc
     2761 gtttacacac gcatatcggg aagacattag gcccttattg aaaggagtcc tttatgaatc
     2821 ttcttatgag ttgtgagcga agctttctac gaaagaccgt cttcacgacg gtgtgtacat
     2881 tagtaggcct cctccatctt cttaccgtct cgctggcgac ggcacaggaa gctaaagagg
     2941 ctcaaaagga attcgatcgc ttcaatgtct cggtgtccct gaaccaggat aagttcttcg
     3001 ggttctatcc gatcattgcc ggcacctatt acctgcgaca ggatttcggt atcgccttcg
     3061 gctctacgct ctatgggcac ttgtctaacc cgaggccgga ctggagcaat ccttggacct
     3121 gggtttatgg tggaatccac aagtccttca tggacggcaa actggtgctc cgtccccagg
     3181 tcggattcac gaacggtcag gtcaac
//
LOCUS       DATNDD010000219         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_676454, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000219 DATNDD010000000
VERSION     DATNDD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676454"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /gene="corA"
                     /locus_tag="VN647_05270"
     CDS             complement(<1..1078)
                     /gene="corA"
                     /locus_tag="VN647_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWG96476.1"
                     /translation="MSRLDLALESAYKHATMKLVQKRSKKTGLSPGTLIHIGQQRTGA
                     VTMTQFNYAGSRCEESVVTDANELRLPSDDIVTWVNVGGVHKVDVLEVFGKYFGLHPL
                     LLEDIANTDQRPKLDDYETYFFLVMKMLMTSERGDIVVEQVSFVLGRNYVLSFQENGV
                     DVFHSVRDRLRGGKGRLRQNGSDYLLYALIDAVVDQYFAVLEMLGERIESLQERVIAD
                     PKPDTLKDIHALKQQLLFVRRAVWPLREAINNLSRSECPLLHEPTKVFFRDVYDHVVQ
                     IVDTIETLREMVSASLDIYLSSVSYRLNTVMRVLTVITTIFMPLTFIAGIYGMNFEYM
                     PELKWPWGYPMALGLMGIVAAVMLI"
     gene            1123..1929
                     /locus_tag="VN647_05275"
     CDS             1123..1929
                     /locus_tag="VN647_05275"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWG96477.1"
                     /translation="MAWDYELDVLIDVIRKAGMEALRFLVDGFETIQKPDQSPVTSAD
                     LAVNQILQFRLASEFPQDGWLSEESPDDLDRLEKRRVWVVDPIDGTRAFISGEPEFCI
                     SVALIEQGQPVVAGIFNPSTDEFFTATRGGGLRLNGKTVTPQDTGGDRKPVVALSPWE
                     CKIRRFRSLEMSTKSHSIRSIAWALAVTASGRIHAAATLEPENEWDVAAGALLIAEAG
                     GAIYDGSGHDLTFNRREPRYSGIIATSPHCPDALTQQLKRLTRTGDDGGE"
     gene            complement(2079..>3104)
                     /locus_tag="VN647_05280"
     CDS             complement(2079..>3104)
                     /locus_tag="VN647_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWG96478.1"
                     /translation="LEGAEKAIRDGHNVYTRFDGVPELRQAIADKLVHYNGIQADPET
                     EVTVSAGATGAFHCACEALLDPGDEVILFEPYYQYHISALRAVEAVAVIVKMRAPKWT
                     FEPAQLEKMITPKTKAIIVNSPGNPSGKIFGRTELEVLADFACRHDLFVMTDEIYEYF
                     LYDGRRHVSMASLPNMAERTVTIGGYSKTFSVTGWRIGYSVAAQPWTKAIGAMNDLLY
                     VCAPAPLQMGVARGIRELRDDFYESLARDYQLKRDQFCSTLAKVGLAPSIPEGAYYVL
                     ADVSRLPGDTGKDRAMYLLEKTGVAGVPGEAFFSGAAGADHIRFSYAKTDADLDEACR
                     RLTQAVF"
BASE COUNT          685 a          883 c          850 g          686 t
ORIGIN      
        1 caatcagcat aacggccgcc acgattccca ttaggcccaa cgccatcggg tagccccacg
       61 gccatttcaa ctcaggcata tactcgaaat tcatgccgta gatgcccgcg ataaacgtga
      121 gcggcatgaa aatcgtcgtg atcaccgtca gcacccgcat gacggtgttc agccgataac
      181 tgacgcttga cagatagatg tcgaggctcg ccgagaccat ttcgcgcaat gtctcgatcg
      241 tatcgacgat ctgcaccaca tggtcgtaga cgtcccgaaa aaacactttg gtgggttcgt
      301 gtaacaaggg acattccgaa cgagacaggt tgttgatcgc ttcccgcagt ggccaaacgg
      361 cgcgccgcac gaacaacagc tgctgtttga gcgcgtgaat gtctttgagt gtatccggtt
      421 tcggatcggc tatcactcgt tcttgcagcg attcgatccg ttcgccgagc atttcgagca
      481 cagcgaaata ttggtcgact acggcatcaa tgagcgcata caagaggtag tccgatccat
      541 tttgccgaag gcgccccttg ccgcctcgca gacgatcccg caccgagtga aagacgtcca
      601 ccccgttttc ttggaacgaa aggacataat tgcggccaag gacgaaactc acctgttcga
      661 ccacaatgtc ccctcgttca gaggtcataa gcatcttcat gacgagaaag aaataggtct
      721 catagtcatc gagcttcgga cgctggtccg tattggcgat gtcttccaga agaagggggt
      781 ggagaccgaa atattttccg aagacctcca ggacatcaac cttatggact ccaccgacat
      841 tcacccacgt gacgatgtca tcggaaggca gtcgaagctc attcgcgtct gtgacgacag
      901 actcttcgca tcgagaaccg gcataattga actgggtcat ggtcacggcc ccggttcgct
      961 gttggccgat gtgaatgagc gtcccgggcg agagtcccgt cttcttcgac cgtttctgca
     1021 ccagtttcat cgtagcgtgc ttgtacgcag attccagagc caggtcaagc cgggacacgt
     1081 tcatttcttt tcatctgcct ccaagcctgt tactctcccg tgatggcatg ggactatgaa
     1141 ctggatgttc tcatcgatgt gatccgaaag gccgggatgg aagcgttacg gttcctagtc
     1201 gatggatttg agaccattca gaaaccggac caatcgccgg tgacctcggc ggacctggca
     1261 gtgaatcaga ttctccaatt tcgcctggcg tcggaatttc ctcaggatgg atggctctca
     1321 gaggaatcgc ctgatgatct ggatcgactt gagaaaagac gagtctgggt ggttgacccg
     1381 atagatggga cgagggcgtt catcagtgga gaaccggagt tttgtatttc ggtcgccctc
     1441 attgaacagg gtcagcctgt cgtcgcaggt atcttcaatc cttccaccga tgaatttttc
     1501 accgcaaccc ggggtggcgg ccttcgtctc aacggcaaga cggttacgcc gcaagatacg
     1561 ggcggggacc ggaaaccggt tgttgccttg agtccgtggg aatgcaaaat ccgccgcttt
     1621 aggtcgcttg agatgtccac caaaagccac tcgattcgtt ccatcgcctg ggcgcttgcc
     1681 gtcaccgcga gcggacggat ccacgcggcg gcgacgctcg aaccggagaa tgagtgggat
     1741 gttgcggccg gagctctcct catcgcagaa gcaggtgggg caatctatga cgggagcggg
     1801 catgatctga cgtttaaccg gcgtgagcct cgctatagcg ggatcatcgc cactagtcca
     1861 cattgtcccg atgctctcac gcagcaactc aagcgtttga ctcgcacggg cgatgatggc
     1921 ggtgaataat gctcccaggc tgttgagacg cctcaggatg ttcaaaaagg ccgtccagca
     1981 aggccgcagc gagcgaagag gcggaatcgt acttggggcc gtacgttgag cctctaagcg
     2041 atgcgagaac gcagctggcg gactttttca acatcctgtt agaagacggc ttgggtcaag
     2101 cgcctacagg cttcatcaag atcagcatcc gtctttgcgt agctgaagcg aatatggtcg
     2161 gctcctgccg caccggagaa aaacgcctct cccggtacac cggccacacc ggttttctct
     2221 aacagataca tggcacgatc tttccctgtg tcgcctggaa gccgggacac atccgccaac
     2281 acgtaatagg ctccttccgg gatcgacgga gcgagtccca ccttggccag agtgctgcaa
     2341 aattggtcgc gcttcagttg atagtctcga gccaggcttt catagaaatc gtcgcgcaat
     2401 tctctgatac cgcgcgccac gcccatttgg agaggcgccg gagcacagac atagagcaaa
     2461 tcattcatcg ccccgatggc ttttgtccac ggttgcgcag ccacgctata gccgatccgc
     2521 caccccgtca cgctgaacgt cttcgagtac ccgccgatgg tcaccgtccg ttccgccata
     2581 ttcggcaagg aggccatgct cacatgccgc cgcccgtcat agagaaagta ttcgtagatt
     2641 tcatccgtca tgacgaatag atcgtgccgg catgcaaaat ctgccagcac ctcaagttcc
     2701 gttcggccga atatctttcc cgatgggttg cctggcgaat tgacgatgat agccttggtc
     2761 ttgggcgtga tcatcttttc gagctgagcc ggctcgaacg tccatttcgg agccctcatc
     2821 ttcacaatca ccgccactgc ctcgactgcg cgcaaggcac tgatgtgata ctgatagtag
     2881 ggttcgaaga gaatgacttc gtcacccgga tcaagcaagg cctcacacgc gcagtgaaag
     2941 gcgccggttg ccccggcgct gacggtcact tccgtttcag gatcggcttg aatcccgtta
     3001 tagtggacca gtttgtcggc gatagcctgg cgtaattcag gcacgccgtc gaagcgggtg
     3061 tagacattgt gcccatccct gatggccttc tctgcccctt cgag
//
LOCUS       DATNDD010000220         6096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3836049, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000220 DATNDD010000000
VERSION     DATNDD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836049"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1203
                     /locus_tag="VN647_05285"
     CDS             145..1203
                     /locus_tag="VN647_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96479.1"
                     /translation="MSEILFCPASLKPTPSPSIATVLYCTTMMPVTPDTELSLSFENQ
                     RAIISPWGASLRRYLLINDGGQETNIVWGYSGGSGKRGGQGDVLIPFPGRVSYGRYTF
                     EGQLLQLECNDKEGPNAIHGFVRSLPWHVQEAHSNKVTFEIRLDADTYAERGYPFSLA
                     VWVTYELGVSGLACTFTVKNVGQQAAPVGAGFHPYFTVETSSIDEAEVQIPGTAYLEF
                     NERLVPTGAILSVIGTPWDYRRFHAVGSQRFNHCYVQLERDAQNMATVSLRHTVRNRR
                     IDIMMDHTFSAVVVYSGDAIADAPRVALAIEPMTCASDAFNHPDWGLKRLVPGETFSG
                     RYSVRYRVIGKDSLCASA"
     gene            complement(1313..1471)
                     /locus_tag="VN647_05290"
     CDS             complement(1313..1471)
                     /locus_tag="VN647_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96480.1"
                     /translation="MIWQDQGKWRGCLQASPDQQVQGESFEELQFKLLQLHRDLISGR
                     PSNLRKIA"
     gene            1573..2472
                     /locus_tag="VN647_05295"
     CDS             1573..2472
                     /locus_tag="VN647_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG96481.1"
                     /translation="MVLNTADAHAVTFQQISANQLSFRVATAGSGDRLILCLHGFPES
                     AVSWRYQIVPLAQAGFLVWAPDLRGYAGTTRPAGIDAYRIESLMDDVTGLLDAAQVQR
                     AILVGHDWGGIIAWYYAMRHTDRVETLVIVNAPHPACFEREVRHWRQWHRSWYMGFFQ
                     IPWLPEAALSACHGYVIGEIFGRMAIHREQMPHDLIQLYRRQACEPGALTAMVNYYRA
                     ALRGGGAMRQRRLGYPAISVPTLVIWGLQDHALVRHNLDGLNDFVTDLTVVKVADAGH
                     FVHEDKPERVTSEMLNWLDLKRV"
     gene            complement(2498..3721)
                     /locus_tag="VN647_05300"
     CDS             complement(2498..3721)
                     /locus_tag="VN647_05300"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWG96482.1"
                     /translation="MNKVLPAWLLLLVLLPAGCGDGGGGGGDGGTFGGVGGGPLGGGP
                     GTTSILYVANNGSDNVSGYSINPTSGTLAAIPGSPFTNVSGPSAMTVSSNGFFAYVTN
                     SRANNVTAFRVSTEGALLLVPPTPANPNPAAVDAAPSALAISPDTKHLYVANNGNDTM
                     TAFNIEAAGALTLIPQTAGNTNPVTVNGADPSSIAIPQSGKFLYAANSGSNDVTAFSI
                     GATGLVSRIAPSGANANPISTGGKVPKDVAISPNGSFLYVANSGSDNLTVFQIGTNGL
                     LTLVPPAGSNPNPVSTGGTTPNALMISLDGRFLYVANGGGNVSAFTIGGDGLLTLVLN
                     SAGNLNPVSAGTNPIALTMSPDGRFLYVANQGGRVSAYTVNTETGSLTPLTALVGNPF
                     LAGTTPSAIATPGRP"
     gene            complement(3988..4827)
                     /locus_tag="VN647_05305"
     CDS             complement(3988..4827)
                     /locus_tag="VN647_05305"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWG96483.1"
                     /translation="MKIRVPSWLALPVALLLSGCPAGGGGDGGFGNGGGISSRIAYVA
                     NSGSNNVFGYMIATNGALTTIIGSPFSGVTAPSAVTVSANATFVYVTNQGGANSVSAF
                     TVNSTTAELTLVANSPFAAGTNPSAVTVSPNGNFLYVANGGSNDVSAYTITTGTGALT
                     PVTNSPFAAGTNPSAVTVSPNGNFLYVANGGDNNVSAFTIDGATGALTSPATIAAGTS
                     PSGVSIDPNGLFLYVTNSGSNNVSAYTITTGTGALTPISGALGNPFSAGTTPLGIATP
                     GRP"
     gene            4980..5804
                     /locus_tag="VN647_05310"
     CDS             4980..5804
                     /locus_tag="VN647_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96484.1"
                     /translation="MHHSLTILGSGYTAKFLLPLARHHYSHVFATSRDPDRHLTHLSP
                     DQRIRFDLARPETWQAIPTSTDIVWCFPAVPIELVRQFAEAASLRTRRLEVLGSTSAY
                     DVCASAEYPPPWVEETAPVDLTKPRVQGEELLRTSYGAIVLRIAGIYGPGRNPVEWIR
                     TGRVNRSRKYVNLIHVEDLVSSCLAALMHADPGGIYNVSDGNPRTWVEICTMVEQRWD
                     IRSPDSQDADSPGKRVSNKRMCELLKLHGIGQCHRNLFETLELMQRSSLNEATPSR"
     gene            complement(5779..>6096)
                     /locus_tag="VN647_05315"
     CDS             complement(5779..>6096)
                     /locus_tag="VN647_05315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWG96485.1"
                     /translation="WGAPPVMAVSILEYVIFAPIFEELAFRGLLFAMLRRRFAFLPAA
                     LISASVFALAHGYSPIGFLSVFWSGFLWAWIYERTGSLIPGMVAHATNNLLVCLTVTA
                     LLR"
BASE COUNT         1330 a         1669 c         1722 g         1375 t
ORIGIN      
        1 ttgtgttcgt cgagaaatgg gcgaacgcgg cgacggaaca tgcgcatttc gccactccac
       61 acgtctctgt tgcactgcaa cggttggtag gacttctggc cgccgagccg gagatctgtc
      121 ggtacaaggt cttaaaatga cgtggtgagt gagattctgt tctgtccagc ttcgctcaaa
      181 cctacgcctt cgccatccat tgcgacggtg ctatactgca cgaccatgat gccggtcaca
      241 cctgatactg aactctccct atcctttgag aaccagcgtg cgattatttc tccctgggga
      301 gcttcgttac gacggtacct gctgatcaac gacggcgggc aggaaacgaa tattgtgtgg
      361 ggttattcgg gtggcagcgg gaagcgaggc ggacagggcg acgtgctcat tccatttcct
      421 ggccgtgtca gctatggacg ttatacgttt gagggacagt tgctgcagct cgaatgtaac
      481 gacaaggaag gtccgaatgc cattcatgga ttcgttcgca gtctgccgtg gcacgtgcag
      541 gaagcccatt caaacaaggt gacgtttgag attcgtctcg atgctgatac atatgctgaa
      601 cgaggctatc ccttttcact tgccgtttgg gtgacgtatg aactcggcgt ctccgggcta
      661 gcctgcacat tcaccgtcaa gaacgttggg caacaggcag cgccggtcgg agccggattt
      721 catccttact tcaccgttga aacgtcttca atcgacgagg ccgaggtgca gattccggga
      781 accgcctatt tagaattcaa cgagcggctc gttccgacag gagcgattct gagcgtgatc
      841 ggcacaccgt gggactatcg acgatttcat gcggtcggca gtcagcgctt caaccattgt
      901 tatgtgcagc tcgaacgtga tgcacagaac atggccacag tatctctgcg tcataccgtg
      961 cgcaatcgaa ggatcgacat tatgatggat cacacgtttt ccgcagtggt cgtgtatagc
     1021 ggggatgcta ttgcagacgc gccacgagtc gcgcttgcca tcgaaccgat gacctgcgcg
     1081 agtgatgcgt tcaatcatcc ggactggggg ttgaagcggc tcgttcccgg tgagacattc
     1141 tctggacgtt atagcgtgcg gtatcgagtc ataggaaagg attctctgtg cgcctcggct
     1201 tgagcatctg ctttggctat ggctgacact cgtcggatgc gtcgaacgag cgagtccgac
     1261 gaccgcttgc actgtcggct agggatcttg gtgtctcagc ggggactatc gctcaggcaa
     1321 tcttccggag gttggaaggc ctgccgctga taagatcgcg gtgaagctgg agaagcttga
     1381 actgcagctc ttcgaacgat tccccttgaa cctgctggtc gggagaagcc tgcagacagc
     1441 cgcgccactt gccctgatct tgccaaatca tgtatttcgt catgtcacgg agtatagctg
     1501 caaggcggag ttccgaacag aattttgtaa atttttaccc aggcgcgcag cgaaatgaga
     1561 acgcatacga gcatggttct caatactgct gacgcacacg ctgtgacgtt tcaacagatc
     1621 tccgcgaacc aactgtcttt tcgagtggca acagccgggt ccggcgatcg attgatcctc
     1681 tgtctacatg ggtttccaga atccgccgtg tcatggcggt atcagatcgt tccgctggcg
     1741 caagcaggat ttttagtctg ggcccctgac cttcggggat atgccggcac gacacggccg
     1801 gcaggcattg acgcctaccg aatcgaatcc ttgatggacg atgtcaccgg tctcctcgat
     1861 gccgcgcagg tgcaacgcgc cattctggtc gggcatgatt ggggtggaat catcgcctgg
     1921 tactatgcga tgcggcacac cgatcgggtg gaaaccttgg tgattgtgaa cgccccccac
     1981 ccggcctgct ttgagcggga agtgcggcac tggcgtcagt ggcatcgttc ctggtacatg
     2041 ggctttttcc aaattccctg gcttccggaa gcggctttgt cggcctgtca tggctatgtg
     2101 atcggtgaga tctttgggcg catggcgatt catcgcgagc agatgcctca cgatctcatt
     2161 cagttatatc gacgacaggc ctgcgagccg ggagcgctca cggccatggt gaattactat
     2221 cgcgcggcct tacgaggcgg cggggcgatg cgtcagcgaa ggttgggata tcctgctatc
     2281 tccgtcccca cactggtgat ctggggatta caggatcatg cactcgtccg tcacaatctc
     2341 gatgggctga atgatttcgt caccgatctg acggtggtaa aggtcgcgga tgccggacac
     2401 ttcgtgcatg aagacaaacc ggagcgggtc acgagtgaaa tgctgaactg gttggatctg
     2461 aaaagagtgt agatatagga tggcttcagc gcctgaccta tggacgaccg ggcgtcgcaa
     2521 tcgccgaggg tgtggttccc gctaaaaagg gattacctac cagagccgtc aatggggtca
     2581 ggctccctgt ttccgtgttg acggtatagg ccgatacacg acctccctgg ttcgccacgt
     2641 acaggaaccg cccatccggt gacatcgtca aggcaatggg atttgtcccc gctgagacgg
     2701 gattgaggtt gcccgccgag ttcagcacga gcgtcaagag tccatcgccc ccgatggtga
     2761 acgccgatac attgcctccc ccattggcga cataaagaaa tcggccatcc agggagatca
     2821 tgagcgcgtt gggagtcgta ccgcctgtgg agactggatt cggattggac cccgcaggcg
     2881 gcaccaaagt taagaggccg tttgttccga tctgaaagac cgtcagatta tcagagccgc
     2941 tgttagccac atagagaaac gatccgttcg gcgagatggc cacatctttg gggactttcc
     3001 caccggtcga gataggattg gcgtttgccc cggacggagc gatcctgctc acaagcccgg
     3061 ttgctccgat tgagaacgcc gtgacatcat tggagccgct gttggccgca tagaggaatt
     3121 tcccgctttg agggatcgca atggatgagg gatccgctcc attgactgtc acagggttcg
     3181 tgttcccggc agtttgcggt atcaacgtca aggcgccggc agcctcgata ttgaacgcgg
     3241 tcatagtatc attgccatta ttcgcgacat acagatgctt cgtgtccgga gaaatggcca
     3301 gtgcgcttgg agctgcgtca accgcagccg ggttgggatt agccggagtc ggcgggacca
     3361 ataagagcgc accctctgtc gaaactctga atgccgtcac attgttcgct ctactgttcg
     3421 tcacataggc aaaaaatcca ttcgaggaca ccgtcatggc ggaaggacct gagacgttgg
     3481 tgaacggaga gccgggaatc gccgcgagag ttccgcttgt cggattgatg ctgtaccccg
     3541 acacattatc ggagccgtta ttcgcaacat agaggatcga tgttgttcca ggcccccctc
     3601 ctaacggccc tcctccaact cctccgaatg tccctccgtc cccacctcct ccacctccat
     3661 ccccacatcc agctggaagg agcaccaaaa gtaaaagcca tgccggcaat actttgttca
     3721 cgatcgaaac cttgtgagct gctcggctgg tcatatgtcg gtttatatca ctcccacgaa
     3781 gcactgacaa cgacgcagtc gggatacggt acggtcgtga tgtgtgatca atatagagtc
     3841 ggtgtgagat gggaacaaaa gaggtggagc gccggttgga tgtcggcggc aacgactgtc
     3901 gtctctaagc tatgtgtcgt cgtgcacgag aagcccgcgg ttcgtggact gaaacgtggg
     3961 cttccccatc tatctggaga agaagactta tggccggcca ggcgtggcga tacccaatgg
     4021 tgtcgttccc gcggaaaacg ggttccccaa tgcgccactg attggggtca gggcgccagt
     4081 tcctgttgtg attgtataag ctgatacatt attactgcca ctgttggtga cgtataaaaa
     4141 cagaccattc ggatcgatgg aaactccgct tggcgacgtg ccagcagcaa ttgtagctgg
     4201 tgatgtcaat gctcctgtag ccccatcaat tgtgaacgcc gacacattgt tgtcaccgcc
     4261 atttgccaca tataagaagt tcccgttggg ggagaccgtt acggcactgg gattagtccc
     4321 agcggcgaac ggcgagttcg ttaccggggt gagagcccca gtccctgtcg tgatcgtata
     4381 ggccgacaca tcgttgctac cgccattcgc cacatataag aagttcccgt tgggggagac
     4441 cgttacggca ctgggattag tcccggcggc gaacggcgag tttgccacaa gtgtgagctc
     4501 ggccgtcgtg ctgttgaccg taaaggccga tacactgttt gctccaccct gatttgtcac
     4561 ataaacgaat gtggcgttgg cagacacagt aaccgccgat ggtgccgtta caccggaaaa
     4621 tggagagcca ataatggtcg tcagtgctcc gtttgtcgcg atcatgtacc caaacacatt
     4681 gttggatccg ctattcgcga catacgctat tctcgagctt attccaccgc cgttcccaaa
     4741 tcctccgtct ccgccaccac ccgctggaca cccactcaaa agtagcgcta ccggaagcgc
     4801 gagccatgac ggtactcgta tcttcatgtg gtgtcctctt gttcgtctcc aatgtgaaat
     4861 gtttattcct cgtataacac gaggaaatac gtacaccaac taaaatgact tttttcgttg
     4921 gactctgatc ggttctcgct ggcacactgc tcacacgtca gcttgggata cgactgatct
     4981 tgcatcattc actcaccatt ctcggatccg gctataccgc caagtttctc ctgcccctcg
     5041 cccgacacca ttactctcat gtcttcgcca ccagtcgaga tccggatcgg cacctcacac
     5101 atctgagtcc tgaccaacgg atacgattcg acctggcacg gcccgagaca tggcaggcca
     5161 ttcctacatc aaccgatatt gtgtggtgct ttcccgccgt accgatcgag ttggtccggc
     5221 agttcgccga agccgcatct ctacgcacgc gccgcctgga ggtactcggc agtacctccg
     5281 catacgacgt ctgtgcctca gccgagtacc ctccaccctg ggttgaggaa accgcgccgg
     5341 ttgacctgac aaagcctcgt gtacaaggcg aggaactatt gcgaacttcc tatggcgcca
     5401 tcgtattgcg aattgcggga atctatggtc ccggtcgcaa tcctgtcgag tggatcagaa
     5461 caggccgagt gaatcgatca cgcaaatatg tcaacctgat tcatgttgag gatttggttt
     5521 cgtcctgcct tgcagccctc atgcacgctg accccggcgg aatctacaac gtcagcgatg
     5581 gaaatccgag gacctgggtg gagatctgca cgatggtcga gcaacgatgg gatattcgat
     5641 cccctgactc tcaggatgcc gactcaccag gaaagcgtgt gtcaaacaaa cggatgtgcg
     5701 agttgctgaa attgcatgga ataggtcagt gccataggaa tctattcgag acgcttgagt
     5761 tgatgcagcg gagttcgctc aacgaagcaa cgccgtcacg gtgaggcaca cgagcaagtt
     5821 gttcgtcgca tgggcgacca taccgggaat cagacttcct gtccgttcat agatccatgc
     5881 ccagaggaat ccgctccaaa aaacgctgag gaatccaatc gggctatagc cgtgggccag
     5941 agcaaagaca ctggcgctga tcagggcggc aggaagaaat gcgaaccggc ggcgaagcat
     6001 cgcgaacagt aatccacgaa acgccagctc ctcgaagatc ggtgcgaaga tgacgtactc
     6061 gaggatactc acggccatga cgggcggagc ccccca
//
LOCUS       DATNDD010000221         3032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1610952, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000221 DATNDD010000000
VERSION     DATNDD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610952"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VN647_05320"
     CDS             complement(<1..335)
                     /locus_tag="VN647_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWG96486.1"
                     /translation="MSKERIGVGLIGVGRHGARYAHHLVQDLPMASLRAVCRRHPEQG
                     FHMPGAESVTVYGDAQSLIADPMVDVVVVVTPPIYSFEICGLAAHARKPLLIEKPLAT
                     TATDARMMVA"
     gene            703..1503
                     /locus_tag="VN647_05325"
     CDS             703..1503
                     /locus_tag="VN647_05325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWG96487.1"
                     /translation="MSTTQTTPFTLEQIGTGTARFPSGIPIPTHTDQMVDPYFKSKIP
                     KEHHIDCLLFWPQQADTFPGLVLLHDWWGLTGQMKDLGARLACEGYTVIIPNLYGRLG
                     GMVTANDDVAAALLERQNDAQVITDINSCCEYLNTRLFVKKNIHGVVGYGMGGSYALR
                     FACHRKRLRAAVSYYGKMVAPKELMKDLFSPVLYHQAGKDTWATQADAEQLRSASADY
                     AKRVEIHLYPDASHAFCNDMKPHAYDPDSSALAWERTATFLKSCFQGL"
     gene            1500..2498
                     /locus_tag="VN647_05330"
     CDS             1500..2498
                     /locus_tag="VN647_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96488.1"
                     /translation="MTPRRSPMHSQPRTPVTIARTLGRNQAHHVHRHTMAGFLLILIF
                     SMLAPSIAMAEGTVSPILLAEVAGQQADQLASIASDEAALTLFIKTVGPALGLKDAAG
                     TLGAKRLPGKMVKELGLAELSNSVYELMAALSAWQFADSLGHDVGQSSSGTPLPASAR
                     QDWLTNRSRVDSLPDLCRLSQAAQTLRTSATAPTQQNNDLPLAALRTVFDSSQQATAA
                     WWEIYGWKERVRQAKGRARLCGTWQWVIHNHQHHEEQKHTLVFPPDGPMSATIPALSE
                     MIMLGDGIYLRWEHNGQIQEDSLLFIKDNTKLEGSFVNSMGGWGAISGKRTAPCRP"
     gene            complement(2499..>3032)
                     /locus_tag="VN647_05335"
     CDS             complement(2499..>3032)
                     /locus_tag="VN647_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HWG96489.1"
                     /translation="LVTHRRAIGETIPYEESSSTGDGGWSIQRGSRMIRPFTTGMYRE
                     PVTMDLDTSSRWLVLVGCICAGLGVAAGAFGAHMLKDLLDQPMLAVYDTATRYQMYHA
                     FGMVLSGFAVRIGRDAGAAKAGWLFLAGILLFSGSLYGVSLLGVRWLGAVTPIGGALF
                     IIGWCVLAWRAWRWAAS"
BASE COUNT          690 a          946 c          788 g          608 t
ORIGIN      
        1 gccaccatca tgcgggcatc agttgccgta gtcgccaatg gcttctcgat caacagaggt
       61 ttacgagcgt gggcggctag tccacaaatt tcgaacgagt aaatgggcgg agtcaccacg
      121 acgaccacat cgaccatcgg atcggcgatg agggattggg catctccata gaccgtgacc
      181 gactcggcac ccggcatatg aaaaccctgt tcaggatgtc gtcgacatac ggctctgaga
      241 gaggccattg gaaggtcctg aacaagatgg tgggcatacc gagccccgtg ccgacccacg
      301 ccgatcagtc ccaccccgat acgttccttg ctcacaagac cctcacaagg tctcactcta
      361 gcaggatgcc gaaagagtcc gccagctttg ttctcgcaaa cacggacgtc tcactaaaac
      421 acggggcgct cacagaacac tgcccgctca cgaagacacc gccgccgcac cgactcggcg
      481 gcgtccacaa acgtgacgct cattattctt cgcgtcgtgg acctcgccgg cgttcacaaa
      541 cgtgaaaagc ttcggacact gccggctcac cgactcgccg gcgcgcacaa acgtggcgct
      601 ctttattcat cgcgccgtgc gcctcatttg acattcccgc aaacggcttc atatagtaag
      661 gccatgttgc gtgcaacgtg gctctgttaa gtcattccga tcatgtctac gacgcaaacc
      721 acccccttta ccttggaaca gatcgggacg ggaaccgcgc gtttcccaag cggcataccg
      781 attcccaccc acaccgatca gatggtggac ccctacttca agagcaagat accgaaggaa
      841 caccacatcg attgcctgct cttttggccg caacaagccg acacctttcc gggtcttgtc
      901 ctattgcatg attggtgggg tctgaccgga caaatgaaag atctcggcgc ccgactggcc
      961 tgtgaaggct atacggtgat cattccgaat ctctacggcc ggttgggagg aatggtgacc
     1021 gccaacgatg acgtagcggc cgctctgctg gaacgtcaga acgatgccca ggtcattacg
     1081 gatatcaact cctgttgtga atatctcaat acccgcctct tcgtgaagaa gaacattcat
     1141 ggcgtcgtcg gttatggaat gggtgggtcc tatgccctgc gcttcgcctg tcatcggaag
     1201 cgattgcgcg ccgccgtttc ctattacggt aaaatggtcg ctcccaaaga gctgatgaaa
     1261 gacctctttt cgcctgtcct atatcaccag gcgggaaagg atacctgggc cactcaggcc
     1321 gacgccgagc aattgcgcag cgcctccgcc gactatgcca agcgagtcga aattcatctc
     1381 taccccgatg cgtcccacgc tttctgtaat gacatgaagc cgcatgcgta cgatcccgac
     1441 agttcggccc ttgcttggga gcggaccgca accttcttga agtcttgctt ccaaggacta
     1501 tgaccccgcg tcgctcccca atgcactctc aaccccgcac accggtcacg atcgcgcgaa
     1561 ctctcggccg gaaccaagct caccacgtcc atcgccatac catggccggc tttctcctga
     1621 tcctaatatt ctcgatgctc gccccgtcga ttgccatggc agagggaacg gtgtctccga
     1681 tcctgttggc cgaagtggcg ggtcaacagg ctgaccagct cgcttcgatc gcctccgatg
     1741 aagcggccct gacgctgttc atcaagaccg tgggcccggc gcttggtttg aaagatgcgg
     1801 cagggacatt gggagccaaa cgactccccg gcaagatggt caaagaactg ggattggccg
     1861 aactgtccaa ctccgtctat gagttgatgg cggcgttgtc cgcctggcaa tttgctgatt
     1921 cactcggtca tgatgtcggt cagtcttctt ccggcactcc attacctgct tcagcacgac
     1981 aggattggct gacgaaccga agccgggtcg actcgctccc cgacctttgc cgtctcagcc
     2041 aagcagccca gacccttcgg acatctgcaa cagctcccac gcagcagaac aacgatcttc
     2101 cgttggccgc tctacgtacg gtatttgact ccagccagca ggccactgca gcgtggtggg
     2161 agatctatgg ctggaaagaa cgtgtcaggc aagcaaaagg ccgcgctcgt ctctgcggaa
     2221 catggcaatg ggtcattcat aaccatcagc accacgaaga gcagaaacat accctggtct
     2281 ttcctcctga cggaccgatg tcggccacta ttcccgcctt gtcggaaatg atcatgctcg
     2341 gcgacggcat ttatttgcga tgggaacaca acgggcagat tcaagaagac agtctgctgt
     2401 tcatcaagga caatacgaag ctcgaaggat catttgtaaa cagcatgggg ggatggggag
     2461 caatcagcgg gaagcgtaca gccccctgcc gaccgtaatc agcttgctgc ccaacgccag
     2521 gcgcgccaag ccaatacgca ccagccgata atgaataatg cgcctccgat cggcgtcacc
     2581 gctccgagcc agcgcactcc cagcaacgat actccgtaca ggctaccgga gaataagagg
     2641 attcccgcca agaacagcca tccggctttg gccgctccgg catcgcgacc gatgcgaacg
     2701 gcaaacccgc tcaagaccat accgaaggcg tgatacatct gatagcgggt cgcagtatcg
     2761 tagacagcca gcatcggctg atccagcaga tctttgagca tatgggctcc gaatgcgccg
     2821 gcagccacgc cgagtccggc gcaaatacat cctacgagca ccaaccatcg agatgacgta
     2881 tcgagatcca tcgtcactgg ctccctgtac atgccggttg taaacggtcg gatcatacgc
     2941 gatccccgct gaatactcca tccaccgtcc cctgtcgagg aactttcctc gtatggtata
     3001 gtttccccta tggcccgacg atgggtaact ag
//
LOCUS       DATNDD010000222         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3065483, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000222 DATNDD010000000
VERSION     DATNDD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3065483"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..995
                     /locus_tag="VN647_05340"
     CDS             387..995
                     /locus_tag="VN647_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWG96490.1"
                     /translation="MDFLTNIEEIRKRARRHIEEGSVTERYALDRKQAIRVLNDALAT
                     ECMCVLRYRFHYFMATGINSAAVAEEFMEHAQEEQAHVDQLAERIKQLGGKPELHPAV
                     IAERSHSEYREGTSLADMIREDLIAERIAIETYREMVRYFGDKDTTSRVMMEGILAKE
                     EEHADELADLLFAVQPDTNQGSRRLYFSDEVPRHAEGEKVKS"
     gene            1037..1558
                     /locus_tag="VN647_05345"
     CDS             1037..1558
                     /locus_tag="VN647_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWG96491.1"
                     /translation="MAKRQMKSNQDDDEPNEAPAESVHALELLKEDHRKVERLFQSFE
                     TADGRSRQGIADEAIKALEIHTKVEDGLVYPTIREATGEEDLVDEATEEHHVVTLVIK
                     ELKKMKASAEGYKAKFTVMSELVRHHIEEEEKEMFPEAENADIDWEELGREALSIKER
                     MMSKSSRGQRRAA"
     gene            complement(1599..1751)
                     /locus_tag="VN647_05350"
     CDS             complement(1599..1751)
                     /locus_tag="VN647_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96492.1"
                     /translation="MKTNRAVVLGQGRRTRVTGLDNRLFVEAVLWIAARTYRGVTCPR
                     ASSGTW"
     gene            1897..2721
                     /locus_tag="VN647_05355"
     CDS             1897..2721
                     /locus_tag="VN647_05355"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWG96493.1"
                     /translation="MTENPSEKERYRTNTQDLGWNEERAEFEDHRENIGDVQRVLSVI
                     AGMVFLASGLFRRNRRRNWSGAALVTAGGGLLYRAISGYCPVFGVVGIDMSSNDQSGR
                     SSDTGRLGRRKVHTDRATKIQRAIEVNRPPNEMYRFWRSLDNLPRIMNHLDSVQVIND
                     RLSHWVVKAMPGVPQVEWDAEIINDVENRRIGWRSLEGADVDNAGSVRFKATGGGRRT
                     WLTVTLQYKSPGGELGDAVAKWFGEDPNTTIAKDLQRFKEEMETGVFSQAGAEGEA"
BASE COUNT          743 a          667 c          880 g          586 t
ORIGIN      
        1 gaagtgttgc gggcatacca ggccacgaga gactaaaccg tacggtaaac ccctgagttt
       61 accctgccag ggaaccgata cgtatggaac tgcgaatcgg gacaccgggt tggcagtgta
      121 aacactgacg acggctgtac tcccgcgacg ccgggtattc ccaatacaga ttgtccttca
      181 cttgacagat tgtcctcaca gcatgtcttg gtttgggtcg cgatcatgtc gaagacacgc
      241 ttcgtcggcg attgtcttca tgttttcaag gttaactcgc gcgcaattct tggtgccggt
      301 gagaaacgat aaggcaaagt ttatgcagct cagggttgag tctcggaaga aatgacgagg
      361 tatcaatagg aagaggggag gaagctatgg atttcctgac caatatcgaa gagattcgga
      421 aacgagcccg tcggcatatt gaagaaggtt ctgtcaccga gcggtatgcg ctggaccgga
      481 aacaggctat ccgagtgctg aacgatgcat tggctaccga gtgtatgtgc gtgcttcgtt
      541 atcgattcca ctatttcatg gcgacaggca tcaactcggc cgcagtggcc gaagaattta
      601 tggagcatgc tcaggaggaa caggcgcatg tcgaccagct cgcggagcga attaagcagt
      661 tgggaggcaa gcctgagctt catcccgctg tcattgcaga gcgtagccac agtgaatatc
      721 gggaaggcac gtctttagcc gacatgatac gcgaagatct gatcgccgag cgtattgcca
      781 ttgagacata tagggaaatg gtgcggtact tcggcgacaa ggatacgact tcccgcgtca
      841 tgatggaagg catcttggcg aaggaagaag agcatgccga cgagctggcg gacttactgt
      901 ttgccgtgca accggacaca aaccagggct cgcggcgact gtacttcagc gatgaggtgc
      961 cgagacatgc agaaggagaa aaggtgaaat cctgatggag caacctgatc gatgcacatg
     1021 caaggaggga gtcgcgatgg caaaaagaca aatgaagtcg aatcaagatg atgacgaacc
     1081 gaatgaggcc ccggcggagt ccgtacacgc actggaactt ttaaaagaag atcatcgaaa
     1141 agtggaacgg ttgtttcaat cgtttgaaac ggcggacggt cgttcccggc aagggattgc
     1201 cgatgaggcg atcaaagccc tggaaatcca tacaaaagtc gaggacggcc tcgtgtatcc
     1261 gaccattcgt gaagcgaccg gtgaagagga cttggtcgac gaagccactg aagaacatca
     1321 cgtggtcacg ttggtgatca aagagctcaa aaaaatgaag gcatcggccg agggttacaa
     1381 ggcgaagttt acagtaatga gcgagctcgt gaggcaccac attgaagagg aagagaagga
     1441 aatgtttcct gaagcagaga atgcggatat cgattgggag gaattaggac gggaagctct
     1501 cagtatcaag gaacgaatga tgtccaaatc ctcccgaggc caacgtcgag ccgcataaga
     1561 atgagaacag cgagccaagg cgcggcatag aaacagggtc accacgtccc tgacgaagcg
     1621 cgggggcagg tcacgccacg gtaagtccgt gcggcgatcc acaacaccgc ttcgacaaag
     1681 aggcgattgt cgagccccgt cacccgcgtt cgccggcctt gcccgagcac aacggctcga
     1741 ttcgttttca ttggtcgtcg cgcaacatct tccggtccat cctcgcaagc tcccgaaagc
     1801 gagatgtacc taagtttgcc ctttgagaac agaccctagt agcttaccta tcacatgaca
     1861 tgaggagaac tcatgaggag gactgaggag gagccgatga ctgagaatcc gtcggagaaa
     1921 gagcgatatc gtacaaatac gcaggatttg ggatggaatg aagaacgagc ggagttcgag
     1981 gatcatcgag aaaacatcgg cgatgtgcag cgggtgctat ccgtcatagc aggaatggtc
     2041 tttctagcct ccgggttgtt tcgccgtaac cggcgccgta actggtcggg tgcggcgctg
     2101 gttacggcag gagggggact tctctatcga gcgataagcg gctattgccc tgtgttcggg
     2161 gtcgtgggta tcgatatgag cagcaacgac cagtcgggtc gatcaagcga taccggtcga
     2221 ttgggccggc gcaaggttca tacagaccgc gcaacgaaaa ttcaaagggc gatcgaggtc
     2281 aatcgtccac cgaatgaaat gtatcgcttt tggcgttcat tggataattt gccccgtatc
     2341 atgaaccatc ttgattcggt acaggtcatc aatgatcgac tgtcccattg ggtggtgaag
     2401 gcgatgcccg gtgtccctca ggtagagtgg gatgccgaaa tcatcaacga tgtggagaat
     2461 cgacgcatcg gatggaggtc gctggaaggc gccgatgtgg acaatgccgg ttcggtgagg
     2521 ttcaaggcga cgggtggtgg acggcggact tggttgaccg tgacgctgca gtataaatca
     2581 ccgggcggtg agctcggcga cgctgtggcg aaatggttcg gggaagatcc gaatacgaca
     2641 attgctaaag atctgcagcg attcaaagaa gagatggaga cgggagtctt ctctcaggcc
     2701 ggcgctgagg gggaagcgta ggtattatgc ccaggaaggc tcgcagcagg tgtgatccat
     2761 atgacctcat cgtattgcct gacgatcatg tagtggggag cgggacggcc tcgcttaccg
     2821 ttatgaccta ctgcgatttc gaatgtccct actgtcgaca agcatgtttt ctcatt
//
LOCUS       DATNDD010000223         3191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1983758, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000223 DATNDD010000000
VERSION     DATNDD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983758"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VN647_05360"
     CDS             complement(<1..1046)
                     /locus_tag="VN647_05360"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWG96494.1"
                     /translation="MQQGVGTIQRALRAVGGHGGWLAGLLLTAGWLALPSIGFAAEGA
                     AAGPTEYRDIPYIGSRNIIWIVAQLHLLLAGFVLGVPIFAWLCEVIAWRGGEQRYDKL
                     AKEFTKLLTSAYATTALFGGILLFLLVSFYPKLMNYLTDVFFPSFLFYCLLFLLETAT
                     LYLYWYGWDAMQHGRMKTVHVFLGFLLNFFALFIMIVPNAWATFQSSPVVISEGTAFE
                     RAWAATWNPTWWPINIHRLIANVVLGGYICGAYAGVRYLSVKSNEDREHYDWMGYVGN
                     FIGVFGLLALPFAGYWLMREIYQYNQQMGITLMGGFLSWLFIIQAMLIGVLFLGSNYY
                     FWLGITYRIPGSEA"
     gene            complement(1072..2082)
                     /locus_tag="VN647_05365"
     CDS             complement(1072..2082)
                     /locus_tag="VN647_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96495.1"
                     /translation="MAEERESMSPTKIGFGILWPACWTGIPIKAVFAVLAMTMGLVHF
                     EGRFGLAFLMLLASPVTVFAAPIIIAVFETHFGEGIGLPLIFGMSIPVDIWALGLVGR
                     TFFLERLRKEPPHDGLGFAIWWRAALIGTFFLPLLWWIVSRVTEIAITASHSMSNMDS
                     LRHLFGTGLPIAERIGLELTIWGSISSAVLIVLAVIGISILGQIIRRMAENSRPASEN
                     YQGLITRWDLMRVPDDQGLLLASLAGVGVVLSLMFWTILPATTPNPHDCCKRPEAHVE
                     PAFKPVESLNKNAQRIASLTAQVEAMEQQKAETKGQKEKGKGKAEGGMAKDSTAKTKP
                     QP"
     gene            complement(2102..>3191)
                     /locus_tag="VN647_05370"
     CDS             complement(2102..>3191)
                     /locus_tag="VN647_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HWG96496.1"
                     /translation="HQPSAIEKLVAFLDERVGLKEMQAKMLNEPIPGGSRWAYVFGSI
                     LLFIFAMQALTGILLMFYYVPTADHAYASTQYIIHDVDYGWFLLGYHFWGSSAMVVCV
                     VAHMSQVFLWGAYKKPRELLWLVGLALFGVVITFGFTGYLLPWDQRAFWATTVGVEIL
                     DKTPFIGDFIARFLKGGPTPGQMTLSRFFVLHVMILPAALMALAGLHLFLFRMAAPAG
                     PFTGTVEEIKAKTDYFFPRQIWKDMVGMAFVFVGICALALWEPVVLLDEAAPDPGDYH
                     PEPEWYFLFYFQLLRLKLFAGEFGQFLGAVALPVAFMALLVALPFIDKDPERNIFKRP
                     IALISWIAIMVVIVLFTVASVINREFLD"
BASE COUNT          817 a          920 c          863 g          591 t
ORIGIN      
        1 tttgcctccg atccgggaat gcgataggta attcccaacc agaagtaata attcgaacca
       61 agaaacagga caccgatcaa catggcttgg atgatgaaga gccaggacag gaatcctccc
      121 atcagggtga tgcccatctg ctggttgtac tgatagattt cccgcatgag ccagtagccg
      181 gcaaatggga gagccaacaa gccgaacacg ccgataaaat ttccgacgta ccccatccag
      241 tcgtaatgct ctcgatcctc gttacttttg accgacagat accggacccc ggcataggcg
      301 ccgcaaatgt acccgccgag caccacgttg gcaatgagcc gatggatgtt gatcggccac
      361 caggtcggat tccacgtcgc ggcccaggct ctctcaaaag ccgtaccttc ggaaatcacg
      421 accggactgg actggaacgt ggcccaggca ttgggcacaa tcatgatgaa caaggcgaag
      481 aagttcagca ggaaccccag gaatacatgc accgttttca tcctgccatg ctgcatggcg
      541 tcccacccgt accaatacag atagagcgtc gcagtttcga gcaggaacag gaggcagtag
      601 aacaagaagg aggggaagaa gacgtccgtc aaataattca tgagtttagg gtagaacgac
      661 actaagagaa acaggagaat gccgccgaac agggcggtgg tcgcgtaggc cgaggtcagg
      721 agcttggtaa actccttcgc gagcttgtcg tagcgttgtt cgcctcctct ccacgcgatg
      781 acttcgcaca gccacgcgaa gatcggcacg cctaagacaa acccagccag cagaagatgc
      841 agctgtgcga cgatccagat gatgttccga ctgccgatgt aggggatatc acgatattca
      901 gtcgggccgg cggcggctcc ctccgctgcg aaaccgatcg aagggagtgc aagccaaccg
      961 gccgtcaaca agagacctgc gagccatccg ccgtgacctc cgacggcccg aagagctcgt
     1021 tgaatcgttc cgaccccttg ttgcatggca ctcacctcat cacctcgcaa gttacggttg
     1081 cggtttcgtc ttggcagtgg agtccttggc cattccacct tcggccttcc ccttgccctt
     1141 ttctttctga ccttttgttt ccgccttttg ctgttccatc gcctcaactt gcgccgtaag
     1201 tgacgcgatc cgttgcgcat tcttgttcaa tgattcgacc ggtttgaagg ccggttcgac
     1261 gtgcgcctcc ggccttttac agcagtcgtg cggattgggc gtcgtcgccg gaagtatggt
     1321 ccaaaacatc agggacagga ccacaccaac ccccgccaag gaagccaaca acaacccttg
     1381 gtcgtccggc acgcgcatca aatcccagcg ggtgatgagc ccttggtaat tttcagacgc
     1441 cggacgggag ttttccgcca tccgccgaat gatttgtcca agtatggaga tcccgatgac
     1501 cgccaagacg atcagaactg ccgacgagat ggatccccag atcgtgagct cgagaccgat
     1561 tcgttccgcg atgggaagac cggtaccgaa gagatgtcgc aagctgtcca tgtttgacat
     1621 cgagtgagag gcggtgatgg caatttccgt gacacggctg acgatccacc agagaagcgg
     1681 aagaaaaaag gtcccaataa gcgccgctct ccaccagatt gcaaaaccaa gcccatcgtg
     1741 gggtggctct ttccgaagcc gttccaagaa gaacgttcgc ccaaccagtc ccagcgccca
     1801 gatatcgacg gggatggaca tcccgaaaat gagcggcagc ccgatccctt caccgaagtg
     1861 cgtctcaaag acggcgatga tgatgggagc ggcgaagacc gtgacgggac tggccaacag
     1921 cattaaaaac gcaagaccga atcttccttc gaaatgcacc agtcccatcg tcatggcaag
     1981 aaccgcaaac accgccttaa tgggaatgcc ggtccagcag gcaggccaca ggataccgaa
     2041 gcctatcttc gttggggaca tcgattcgcg ttcttcagcc atgaccaaac gtgtaccagg
     2101 atcagtcgag gaattctcgg ttaatcaccg atgcaacggt gaacaagacg atgaccacca
     2161 tgatcgcaat ccagctgatg agggcgatcg gccgcttgaa aatattccgc tcgggatctt
     2221 tgtcgatgaa cgggagcgcg acgagcaatg ccatgaaggc gaccggcaac gccacggcac
     2281 ccaaaaactg accgaactca ccggcgaaca gtttcaacct gagcagctgg aagtaaaaga
     2341 gaaaatacca ttccggttcg ggatggtagt cccctggatc cggtgccgcc tcatccaata
     2401 agacgaccgg ctcccagaga gccaaggcgc agatccctac gaagacgaac gccatcccga
     2461 ccatgtcttt ccaaatttga cggggaaaga aatagtcggt tttcgctttg atctcctcca
     2521 ccgtaccggt gaagggcccg gcgggagcag ccatgcggaa caggaacaga tgcaatccgg
     2581 ctaaggccat gagcgccgcc gggagaatca tcacatggag cacgaagaat cggctcaaag
     2641 tcatctgtcc cggagtcggc ccgcctttca gaaatctggc gatgaaatca ccgataaagg
     2701 gagtcttatc caagatttcg acacccaccg tcgtcgccca gaaggcccgt tggtcccagg
     2761 gcaagaggta gcccgtaaag ccgaaggtga ttacaacacc aaacaaagcc agtccgacaa
     2821 gccacaacaa ttctcgcggc ttcttgtatg ccccccagag aaacacctga gacatgtgcg
     2881 ccaccacaca gacgaccatg gcgctggatc cccaaaaatg gtagcccaaa aggaaccatc
     2941 cataatcgac atcgtgaatg atgtattgcg tgctggcata ggcatggtcg gctgtcggca
     3001 cgtaatagaa catgagcaag atgccggtca gtgcctgcat ggcgaagatg aacaacaaga
     3061 tggacccgaa cacataggcc caccgggaac cgccggggat cggctcgttg agcatcttgg
     3121 cctgcatttc cttgaggccg acgcgttcgt caaggaaggc cacgagcttt tcgatcgcgc
     3181 tgggctgatg g
//
LOCUS       DATNDD010000224         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3298369, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000224 DATNDD010000000
VERSION     DATNDD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298369"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..409
                     /locus_tag="VN647_05375"
     CDS             143..409
                     /locus_tag="VN647_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96497.1"
                     /translation="MKAAAQSICQATSLTEVGRVDIIFARRWFEVYSMFVARLLQSLS
                     GFFAFLQCQRTLLGRLRTMNIIERCFVGVRRRTRPMSGTDYREV"
     gene            complement(848..1009)
                     /locus_tag="VN647_05380"
     CDS             complement(848..1009)
                     /locus_tag="VN647_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96498.1"
                     /translation="MYVWRTNSEKLIEELKDSLNLDTLCLQTLDATDVTFLDRQRSRQ
                     PSYLWAFER"
     gene            complement(1647..1949)
                     /locus_tag="VN647_05385"
     CDS             complement(1647..1949)
                     /locus_tag="VN647_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96499.1"
                     /translation="MKIVGRCVFTILCLGLSTLSDWSTTETRAAGQQAQHPAGIASSQ
                     KAETQLYDATADYEGGPVARRSPPTFGNGERKGIAHLESSGQDGTAHHSLNILRVE"
BASE COUNT          593 a          727 c          727 g          672 t
ORIGIN      
        1 tatctcgagt tgatatgacc gataggtgtt caggcttgac cccggtgctc caggcggtct
       61 gtccgatcgc ttcgcatcag cattgttggg tacacaagcc tcgtagtctg ttgaatgaca
      121 ccaaacgacg ggtcacgtcg cgatgaaggc ggctgcccaa tccatttgtc aggcgacgag
      181 cctgaccgaa gtggggcgtg tcgacatcat cttcgctcgc cgctggttcg aagtgtactc
      241 gatgttcgtg gccaggctgc tccagagtct ctcaggattt ttcgcgttct tgcagtgtca
      301 acgcacactc ttgggcagac tgcggaccat gaatatcatt gagcgctgtt tcgttggagt
      361 gcgccgacgg acccgaccca tgagtggaac ggattatcgt gaagtctgag gatccaccat
      421 taagtaatcg atgatcggtc tcaaccgcac tattcctttc cgatcgaact aaacgctcgc
      481 atcatgagga ggcgaaatct tgttggacat gtccaacaaa ttattgatgt cgtccttgat
      541 catgaggcgt ctccatggtc tggctatagt tggtatgagt cgagcggcct agacggcttc
      601 gactacccag ccgtcagtga catccggtgc gcgagagagc aaaatcagaa tcgtcagcat
      661 cggtcagatc tcgctccagc ttcgtcgcaa ttgacaccca tctgaaagcg tccgcaacca
      721 gtggagaagc tcggcccgct catccgtgag catggccgtc gagcttcttg aatcagatcg
      781 gctcagatca ggaaagccca atcgcatatc gctcgtagtc gtcgtttaag ccagcacgcg
      841 gggagcacta tctttcgaaa gcccataagt aagaaggttg tcgagagcgc tgccgatcca
      901 gaaacgtgac atcggtcgcg tcgagcgtct ggagacagag tgtgtccagg ttcagactat
      961 ccttgagttc ctcgatcagc ttctcactgt tggtgcgcca gacatacata ttgatcaccg
     1021 cttctgcgcc tgcagcacgc ctatggctat gagctgattg ttcgagcctt cgcgtacgac
     1081 acgagcactg aagccagcgc cgttcacagc cccttatcaa ttcaaggtat acgcgcagca
     1141 agagaatcca ggtgtattcg ccaacctctg ctggaagtca cagttgtgga ggcatgtgcc
     1201 gcgcgtgtct accgaagtct tggtgtagga agccgtattt cggcgagctg tcgccgaaga
     1261 aacaccatgg accacggcgt caaatcgtat ggctccatct cagtaatcgc tgatcgccct
     1321 taatcgctcc atctatttcg gttgaacgag atcctggcag catgaagagg gcgaaatgtt
     1381 gttggacatg tccaacagtt taccttgacg ctgttaagca gtcagacatc tgtgcctgca
     1441 tgcgctggat acagccgggt ccccacggtt gtccggggca cgacgtgcca ggggccgagc
     1501 cggatccctg tcgagaatcg ctcctcctac cggatcctga tgggtgactg gtacggctca
     1561 gtgtggagcc gacaccattg acaaggacaa ccgggatacg gctgagtcac gcgaccggaa
     1621 tggcgaagga gacgtcatct tagatctcac tccactcgca gaatattgag ggaatgatgt
     1681 gccgtgccat cttgaccgga actttcgaga tgggcgatcc ctttcctctc tccattacca
     1741 aacgttggcg gtgagcggcg ggccacaggc ccgccctcat aatcggcggt tgcgtcatac
     1801 agctgtgttt ccgctttctg agatgaagcg atgcctgctg gatgttgtgc ttgttggcca
     1861 gcagcgcggg tttctgtggt actccaatcg gagagagtgc ttagtcctaa acagaggatc
     1921 gtaaacacac acctccctac aattttcatg atgactctcc tgctggtggc cgaacggcgt
     1981 attaagtatc agggattgaa aagcacacga aatccctcct caatcttcgc tataatcctg
     2041 ccacacgact gatctttgta ttgtccctcc tcctattcca ttagctgtca ttgcacgtct
     2101 gtggtgtccg gtatggctat tacttacatc ccacggttcg gatggaagcc gatcccttga
     2161 ttgcttgccc actaaaagtt attcccgtaa gggagacgga agtgatccca catacaatgc
     2221 cggtatcttg cgtgcggaag tgtaaaacca ggtccgtctt accatctcca ttgatatcct
     2281 cgttatgacc gcgtccatgc gtctcttctg ctttggcggg cccgaattta actgactggg
     2341 ggtcaaccgt ggtggcatcg aaggttatcg ttgacaggat ggccaccggt atcacccctt
     2401 tgctcttggg attgatgctt gttgcatttg ggaagctgcc gggatgaatg acaatctcga
     2461 ccgtagtaat cgccttacac ttacctgcac tgcctgcgtt gaagatagcc aggatttccg
     2521 ttaaagcctt atatgccaca aatagggcag gggtaagctg gccaggattt catggacacc
     2581 caagttaagg tccacccatc cgtgtgatac ggtcggtgat gaaccagttc attctttagt
     2641 gtgccgaaga agcttttcgc cagcattgtc gtagcacttg ccttgggccg atcgtataag
     2701 cgcggcacgg gttttctat
//
LOCUS       DATNDD010000225         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4055150, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000225 DATNDD010000000
VERSION     DATNDD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4055150"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VN647_05390"
     CDS             <1..204
                     /locus_tag="VN647_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96500.1"
                     /translation="GFIAWWLWRTIYLLKLPRFEKKVLVALDWTLDVLFSKDLVHFRT
                     TRPSTLRPTEGELESAAVQVSRR"
     gene            complement(262..468)
                     /locus_tag="VN647_05395"
     CDS             complement(262..468)
                     /locus_tag="VN647_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96501.1"
                     /translation="MGSGGRKTVAVSRGLIMLCDTCYDQVIAEKLPDAKNFVADFEAL
                     FDTICIGCTDINRPLIDDMAGSGE"
     gene            complement(472..666)
                     /locus_tag="VN647_05400"
     CDS             complement(472..666)
                     /locus_tag="VN647_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96502.1"
                     /translation="MVLLYESDPLDDEHARGRFYHVCRGRIDRTPLNVPPEDHPYECT
                     QCGIDLEAEDFFIALRKGSV"
     gene            complement(820..>2001)
                     /locus_tag="VN647_05405"
     CDS             complement(820..>2001)
                     /locus_tag="VN647_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG96503.1"
                     /translation="GYYRETRQSAAELNGYLQDALSGIRETMGFGRQGHEQARFDRLS
                     RTYSDKNLNAMVLWSMYSPGMILLGALGTVLILWYGAGEVMEGRLTLGELVLFLSYLA
                     MFYVPINQIHSVNHMLQHALAASERVFDVLDTIPEVADRPYAVVPVHRAHGAVRFDEV
                     RFHYRPDVPVLRRFSVAVPAGERVALVGMSGAGKSTLLKLLMRFYDVTEGAIIIDEKD
                     VRDLSIAYLRQQIGFVQQEPFLFNGTVRDNLLYGDLHADQARLEAAARAARAHEFITA
                     LPEGYDTWIGERGVKLSVGQKQRVSIARVLLKDPPIVIFDEATSNIDTETEVKIREAL
                     AELTMGRTTFIIAHRLSTLHDVDRILVLDKGRLVEEGRHDALLSRGGVYAGLYEAQFQ
                     V"
BASE COUNT          459 a          604 c          503 g          435 t
ORIGIN      
        1 ggcttcatcg cctggtggct gtggcggacg atctacttgt tgaaactgcc tcgctttgag
       61 aagaaagtcc tcgttgcctt ggactggacc cttgatgtgc tcttttcgaa ggatctcgtt
      121 cacttcagaa cgactcgccc gtctacctta cgaccgacgg aaggggagtt ggaaagcgcg
      181 gcggtccagg ttagtcggcg ctgatcggta tcaatgggtt acgtggtggt gactttacca
      241 acctgaaaaa acgacaggaa gctactcgcc gctcccggcc atgtcgtcga tgagagggcg
      301 gttgatgtcg gtgcagccga tgcagatcgt atcgaagagc gcttcaaaat cggcgacaaa
      361 attctttgcg tccggtagtt tctcggctat cacctgatca tagcaggtgt cgcagagcat
      421 aatgaggccg cgcgagaccg ccactgtttt ccttcccccg ctacccatgc actacaccga
      481 tcctttccga agggctataa aaaaatcctc ggcttcgaga tcaatcccgc attgtgtgca
      541 ttcatagggg tgatcctccg gcggaacatt caacggtgta cgatcgattc gcccccgaca
      601 gacgtggtag aagcgccctc gcgcatgttc gtcatccagc ggatcgcttt cgtacagcag
      661 caccattgat cgaccttgcc tgattgttcg ctccaaggag tataccgacc tcatcttccg
      721 actcgcaacc gccgagaatg cgaaaggctg aggttcaggt cgaggttgag actgagcaca
      781 gacaagactc ggcctacttc gtcgcaacct cacaccgacc tacacctgaa actgagcctc
      841 gtacagacct gcatacactc ctcctcggct gagcagtgcg tcatgtcggc cttcctccac
      901 taatcgacct ttatcgagaa ccaaaatccg gtccacatcg tgaagggtgg ataatcgatg
      961 ggcaatgatg aacgtggttc gccccatggt gagctcggcc aatgcctcgc ggattttcac
     1021 ctcagtctcc gtatcaatat tggacgtcgc ctcgtcgaaa atgacgatgg gcggatcttt
     1081 caacagaacc cgcgcgatgg agactcgctg tttctgccca accgacaatt tgacacctcg
     1141 ttcaccgatc caggtatcgt acccttcagg caacgccgtg atgaactcat gcgcgcgagc
     1201 ggcgcgcgca gccgcttcca gccgagcctg gtccgcatgc aaatcaccat ataaaagatt
     1261 gtctcgaacc gttccattga acaagaacgg ctcctgttgt acgaacccga tttgctgccg
     1321 gaggtatgca atcgacaagt cccgaacatc cttttcatcg ataatgatcg ccccttcagt
     1381 gacgtcgtaa aaccgcatca acaacttcag gagtgtgctc tttcccgccc cgctcatccc
     1441 gaccaatgca actcgttctc ctgccggtac ggccactgaa aatcgcctca gtaccggaac
     1501 gtcaggtcga tagtggaacc ggacctcgtc gaatcgaact gctccatggg cccgatggac
     1561 cggaacaact gcatagggcc gatcggcgac ttccggaatc gtatccagca catcaaagac
     1621 ccgctcactc gccgccaacg catgctgcaa catatgattg accgaatgga tttgattgat
     1681 gggaacgtag aacatcgcca gataggacag gaacaacacc aactcgccga gtgtgagacg
     1741 gccctccatg acctctcccg ccccgtacca gaggatcaag acggtcccga gggctccaag
     1801 taaaatcatc cccggcgaat acatcgacca cagaaccatc gcgttgaggt tcttgtcgct
     1861 atacgtccgg ctcagccgat cgaaacgcgc ttgttcatgc ccttgacggc cgaatcccat
     1921 cgtctccctg attccggaca aggcatcttg tagatagccg ttgagttcag cggcgctctg
     1981 gcgggtctcc cgataatacc c
//
LOCUS       DATNDD010000226         2574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2708367, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000226 DATNDD010000000
VERSION     DATNDD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708367"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..809
                     /locus_tag="VN647_05410"
     CDS             171..809
                     /locus_tag="VN647_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWG96504.1"
                     /translation="MTALIRKTFSVPPLGCNCSIIGDPVTKEAVVIDPGGASERILQE
                     VQRLGFTVSRILHTHAHLDHFLASSEIKKATGAAICLHQEDLELWKNLELQCRVFGVA
                     YVPALPPDHWLADEEKVMLGQVPIVALHTPGHTPGSMSFHVPNDKLLLAGDTLFRGSI
                     GRTDLWGGNFETIEESIRERLYTLDEATTVVAGHGPDTEIRIEKESNQFVRV"
     gene            828..1334
                     /locus_tag="VN647_05415"
     CDS             828..1334
                     /locus_tag="VN647_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWG96505.1"
                     /translation="MASCKLSTVVALLWLVPVYFVARADEPFRSNSDPVKIAEVTVRM
                     SDHGPVVLLQAEDRTIPIFVDLTVALSIQSALNGEKLPRPMTHDLMHTILDAYGGKVT
                     QTVITLKNGTYYAALTVAFKDQVKVFDSRSSDSIALAVHFKAPILVGRDLLESAGKIP
                     EKEKGSEL"
     gene            complement(1338..1646)
                     /locus_tag="VN647_05420"
     CDS             complement(1338..1646)
                     /locus_tag="VN647_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96506.1"
                     /translation="MNQSGWTLATLIISLAASVPVASAADPPCDKYPPTKQSRCTEIW
                     NELNKEDGPLIAQFGLDQQKRRDEGKINAQQHLAENMIFIKQSTEKRIERLKERMAKE
                     "
     gene            complement(1897..2328)
                     /locus_tag="VN647_05425"
     CDS             complement(1897..2328)
                     /locus_tag="VN647_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96507.1"
                     /translation="MTHGVGQLRLGSIALLLGLVSLSGCGTWWHGDHQSVTIFTTPLD
                     AQVVVDDHVHMLAPGTVSLNRKEDHQAVISKDGYEPKTMILTRTWSWWVLGDIFGCAI
                     IFSSACISTDKDDGGYYTFDDKVYVTLEQKSTAAAPDQLNQ"
BASE COUNT          548 a          665 c          724 g          637 t
ORIGIN      
        1 catggatcaa cgaacgtttc attgcgcacg cgatagtcct ccccatagag tcgagagatt
       61 atctacggtt ccggtcaact tgtccacgtg gtattcactt tcgagccctg atgaggtgtg
      121 ctgtccttcg cttgcgctcc cggctcattg gactgtagca tgctcgtatc atgactgcct
      181 tgattcggaa gaccttttca gttccacccc ttggttgtaa ttgctcgatc atcggcgatc
      241 ctgtcacgaa ggaggcggtc gtcatcgatc ctggtggtgc atcggagcga attctccagg
      301 aagtgcagcg attaggtttc accgtcagcc gcatcctgca tacgcacgcc cacctcgatc
      361 actttctggc ttccagtgag atcaaaaaag cgaccggtgc ggcgatttgc ctgcatcaag
      421 aggatcttga actttggaaa aacctcgaac tccaatgccg ggtcttcggg gtcgcgtatg
      481 tgccggccct acctcctgac cattggctgg ccgatgagga aaaggtgatg ttggggcagg
      541 tgccgatcgt ggcgctccat acccccggtc atacgcctgg gtcgatgagc tttcatgttc
      601 cgaacgacaa actcctcctg gccggagaca cgctcttccg gggaagtatc ggtcggaccg
      661 acctatgggg gggcaacttt gaaaccatcg aggaatcgat acgcgagcgg ctctatacac
      721 tcgatgaagc cactacggtt gtggccggac atgggccgga cactgaaatc cgtattgaaa
      781 aagaatcgaa tcagtttgtt cgtgtatgac ggcatggaga gtggaccatg gcttcatgca
      841 agctgtcgac agtcgtcgcg ctgctctggc tagtaccggt ttattttgtc gcccgggccg
      901 atgaaccctt ccgttccaat tctgacccgg tgaaaattgc agaagtgacg gtccgtatgt
      961 cggaccatgg tccggtggtg ctcctgcagg ccgaagaccg cacgattccg atttttgtcg
     1021 acctcacggt ggccctctcg atacagagcg ccctgaacgg ggaaaagttg cctcggccaa
     1081 tgacgcatga cctcatgcac acgatcctgg atgcctatgg tggaaaggtg acgcagacgg
     1141 tcatcacgct gaagaacggg acatactatg cggcgttgac tgtggcattc aaggatcaag
     1201 ttaaagtgtt cgatagccga tcgtccgact ccatcgcgct ggccgtgcac ttcaaggcgc
     1261 cgattcttgt ggggcgtgat ttgctggaat cagccgggaa aattcctgaa aaagagaaag
     1321 gctcagagct gtagcggcta ctccttggcc atgcgttcct tgagccgttc gatgcgcttt
     1381 tcggtcgatt gtttgatgaa gatcatgttc tcggcaagat gttgctgggc attgatcttt
     1441 ccttcatcgc gccgtttttg ttgatcgagc ccaaactgcg cgatgagcgg tccgtcttcc
     1501 ttattgagct cattccagat ctcggtacag cgtgattgct tggtcggcgg atacttgtcg
     1561 cagggcggat cggcagccga agcaaccgga acagaggctg ccaagctgat gatcaaggtt
     1621 gctaatgtcc aaccggattg attcatttct ttttccacca gaatggccgg tcgtccgccg
     1681 aagcaccata ccacaagtcg cgatatcgcg cagacgattc tgacttatga gaaggacgca
     1741 gacaatcaga gaaatagctt ccacaattgt tttgtttgat agatccagcg ggtcattcgt
     1801 tgcgtgatct gagcggttgc ttttacctag ccggaaaaat caacgggtgg aatattttcg
     1861 tggaaaccaa atatagtttc cggtatcacg gcagtgttac tgattaagct gatcgggtgc
     1921 tgctgcagtc gatttctgtt ccagcgtcac gtagaccttg tcgtcgaagg tatagtagcc
     1981 gccgtcgtct ttgtcggtgg aaatgcatgc agatgagaat atgatggcgc agccgaagat
     2041 atcgccgagg acccaccagg accaggttct cgtcagaatc atagtcttgg gttcataacc
     2101 gtctttggag atgactgcct ggtgatcctc ttttcggttg agagagacgg tgcccggcgc
     2161 cagcatgtgg acgtggtcat ccaccacgac ctgcgcatcg agtggtgtcg tgaagatggt
     2221 gacggactga tggtcgccgt gccaccaggt tccacagccg gagagtgaga caagtcctaa
     2281 gagcagagcg atactcccaa gtcgtaattg gccgaccccg tgcgtcatag cgatttctct
     2341 tcgctgatag gtttcctgcg aggcattttt tcactgacca ggctgttctt ctagcggatt
     2401 cacaaaactt gcaaaggggg ctgaggaaga ggcgaccggc taaatcttca tggcctcatt
     2461 cacctcgtgc agggtcgtac cggcgaccgc agatgctcgt ttgctgccgg tctccacgat
     2521 ttcttcaacg cgggatggat gttgagtcaa tttcgtgcgt gcatcccaga tcgg
//
LOCUS       DATNDD010000227         5205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1180940, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000227 DATNDD010000000
VERSION     DATNDD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180940"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..203)
                     /gene="ffs"
                     /locus_tag="VN647_05430"
     ncRNA           complement(104..203)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN647_05430"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(312..401)
                     /locus_tag="VN647_05435"
     tRNA            complement(312..401)
                     /locus_tag="VN647_05435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(365..367),aa:Ser,seq:gga)
     gene            573..713
                     /locus_tag="VN647_05440"
                     /pseudo
     CDS             573..713
                     /locus_tag="VN647_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1029..1166)
                     /locus_tag="VN647_05445"
     CDS             complement(1029..1166)
                     /locus_tag="VN647_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96508.1"
                     /translation="MERWTDVQLVKVIVVILVIIWGVAMLMNGPLDRLLGFFGMNFSP
                     H"
     gene            1451..1636
                     /locus_tag="VN647_05450"
     CDS             1451..1636
                     /locus_tag="VN647_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96509.1"
                     /translation="MQEVIGCSKGGLIIKIHTAVDALGNPVRPGQGPDCTQALLLPEE
                     FTTQVMITDKAMTLTKS"
     gene            2159..2241
                     /locus_tag="VN647_05455"
     tRNA            2159..2241
                     /locus_tag="VN647_05455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2193..2195,aa:Leu,seq:tag)
     gene            2279..3595
                     /gene="tig"
                     /locus_tag="VN647_05460"
     CDS             2279..3595
                     /gene="tig"
                     /locus_tag="VN647_05460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWG96510.1"
                     /translation="MKMEVTELGPMKRALKIEIPADEVTQQFSRAYLELNRQVQIPGF
                     RPGKAPLAILEKRYAKTVEEDVIRKLVPDFYGRAIKQAGISPVVVDIPPLDRVKIKKD
                     SPFTFTATVEIKPTIELRDYKSPNPISLQADKRTVAEEQVDRALEVLREQQARLDAAP
                     PGSALAEGDYAIVDLEGFLDGTPLEGTKKEGQLHRMGSKAALLGIEIDTHLLGKQEGD
                     VVEIPQTYPVSHPDQRVAGKAVRFHLLIKGVKQKKLPTLDDEFAKDCGPYASLHELRD
                     KLRDQMEKALKKDIEESYKDSLLKRLIDTHHFDLPDTLVERELSTIVRQKLQAQQRGK
                     VTDSLPAPDAEELKRIREEHRQEANRRVKAGLILEAIAEKEGLSVSQDDLNNEVTRLA
                     AELRVPMADLVKMIQAGGQDSVEELRARILADKALDVVYRQAVIQG"
     gene            3660..4298
                     /gene="clpP"
                     /locus_tag="VN647_05465"
     CDS             3660..4298
                     /gene="clpP"
                     /locus_tag="VN647_05465"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWG96511.1"
                     /translation="MLVPIVVEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLVI
                     AQLLFLEAEDPEKDINLYVNSPGGSVTAGLGIYDTMQYVKPPINTICLGQAASMGALL
                     LTAGTKGKRFALPNARVMIHQPLGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     IEKIAHDTERDYFMSGEEAKRYGLIDEVITRPPKFMKAVESSDGVKDGIKGK"
     gene            4312..>5205
                     /gene="clpX"
                     /locus_tag="VN647_05470"
     CDS             4312..>5205
                     /gene="clpX"
                     /locus_tag="VN647_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWG96512.1"
                     /translation="MAKQEKMDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPAEIKHHLDQYVIGQERAKRILSVAVHNHYKRISSKEKE
                     VDDIELQKGNILMVGPTGTGKTLLAQTLAKYLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQAADYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTGNILFICGGAFVGLEQIIEQRLNRKAMGFGAEIRGRSD
                     MRLGDLLAKVQPEDFLKYGLIP"
BASE COUNT         1404 a         1277 c         1377 g         1147 t
ORIGIN      
        1 gccttcagga gtgagagccg agatgtaagg aatgtaaaac aagacgatat aaaaccgacg
       61 acgctgtcgc cttacacctc atgctcacgc ctcacgtcca aagtggggcc aggtgatcct
      121 gcggcaccca gaactggctg cttaccgttg cttccttccg gatctggcgg ggttcacaga
      181 gttctattgc acagggccca gcccctatgc tcgatttaga cctccaatat agtccacact
      241 tgaggtgtct attcgtccgc agaaattacg atacagcaat aacgagcgga cggtacacac
      301 gaagtgcggt atggcggaga gggtgggatt cgaacccacg gtcccgttgc cgggacgcat
      361 gctttccaag catgtcgatt cgtccactct cgcacctctc cgcatcgaga agtgcaaagg
      421 gatagcatct tagcatgcgt gtccatgacc ggcaaggtga ggaccaccgt gtagttaagg
      481 ctctgtcgac atttagcgaa aagtgctcga gaatcttctc accaaggagg gtctcatgga
      541 cgagccgagg catgggcgag aggatcaacg cgctgtataa ggagcggaat ctggtggaac
      601 ggatgtgtgg gtacctcaag catttccgtc gggtggcaac gcggtacgac acgcccgcgc
      661 tctccttcct gtcgttcgtc cagctcgcga ccagttacgt atggctcaag taaatgtcga
      721 cagagcctag ggtttggatt ctctttcgtc catcacggtc ttcgagtttc tcgtctttca
      781 ttccttgagt cctactccaa gtgcaacact tcaagcccag atgggctacg gtgttgccat
      841 gcaaacgaga tcgtctgaca cctgagtggc taaggacgag ccgaccctat gacagacaag
      901 atcgtgggcg agctaccaaa gatgcggcgg atatgattga gttagtgaag aaggccgatg
      961 gctgacaatc ggcgccctac gtgatcgtca taacatttca agggataggc tctgcaattt
     1021 ctgacgtatt agtgagggga aaaattcata ccgaagaagc cgaggaggcg atcaagaggg
     1081 ccgttcatta acatcgcaac tccccagata atgaccaaga tgacaacaat caccttgacc
     1141 agttgcacgt cggtccagcg ttccaaggta acccttccta ctctagaagg gcagtaccat
     1201 agcataatgc aatgccataa ctgcacctaa ataataaact ccgtatatgt taaattaagc
     1261 gttattaagt actaatgatt caattctgtt cggaatcaga atgacatgat gggtattcga
     1321 ggtcacccct taaactttaa ccgctttata ttatggtaat accgcattga agacactgta
     1381 cccttgacga ttaaggtact gatgaaagcg ctgcagaccg attgcgttga tcaacgaaat
     1441 tcctactcag atgcaagagg tgatcggctg cagcaaaggc ggtctgatca tcaagatcca
     1501 tacggcggtc gatgcgttgg gcaatccagt gcgtccgggg caaggcccgg attgtacgca
     1561 ggccttgctc ttgccggaag agttcaccac acaggtcatg attaccgaca aggctatgac
     1621 tctgacgaaa tcgtgaactg gatcgcttcc cgcgacgcac aggtcgtcat cccaccacgc
     1681 tcaatccgca agacgtcaag gaattacgga gacactctag gcggaacgac accgtattga
     1741 acattacttc aacaggctca aacactatcg cggaatttcc acaagatact gcaaatgcgt
     1801 cagaaacttc gcgtccttca tctacctcgc agcctcggtg cacccggtgc aatggttgct
     1861 gtaattgtca acaggacaga tcctgctgcc gaagccggca aatttcttta ccaagaaagt
     1921 tatcggaggt gtaccacggc gaacgtatgg gtgaagtttt ttgatgagca acgaggatgg
     1981 acgagatcag caaacagcct gactggacca ttcatactag aatagggcac ccccggtcgt
     2041 atgggttgac attacaggtg tccaacggta caacgctata cgacatcgct gttttttgca
     2101 gtctcggtca cttttctcat agttagagtg acagctccgc agcattctta acaaccatgc
     2161 gagagtggcg gaactggcag acgcgcgaga cttaggatct cgtgggcaac cgtgggggtt
     2221 caagtccccc ctctcgcacc actagtacga ttccttggcg aggagtccaa tctcgacgat
     2281 gaaaatggaa gtgaccgagt taggaccgat gaaacgcgcc ttgaaaatcg agataccggc
     2341 cgatgaggtg acacaacaat tttcacgagc gtatttggaa ctcaaccgcc aggttcagat
     2401 tccagggttt cgaccgggga aagccccttt ggctatttta gaaaaacgct atgctaaaac
     2461 agtcgaagaa gacgtcattc gaaagctcgt tcctgatttt tatggtcgag ccatcaagca
     2521 ggccgggatc agtccagtag tggtggatat tccccctctg gatcgggtca aaatcaaaaa
     2581 agactcacca tttactttta cggcgacagt cgaaatcaaa cccacgatcg aactacgcga
     2641 ctataagtcc cccaatccca tttctctcca ggccgacaag cgtacagttg cggaggaaca
     2701 ggtagatcgt gccttggagg tattgcgtga acaacaggcc cgcttggatg cggccccacc
     2761 cggttcggcc ttagccgaag gagactatgc catcgtcgat ctcgaaggat ttctggacgg
     2821 aactcctctc gaagggacta agaaagaagg gcaactacat aggatgggtt cgaaggccgc
     2881 cttgctgggg attgaaatag atacccatct tcttggaaaa caggaagggg atgttgttga
     2941 gattcctcag acttatccgg tgagccatcc ggatcaacgg gttgccggga aagcagtgag
     3001 gtttcaccta ctcataaagg gagtcaaaca gaagaaactc cccacccttg acgatgagtt
     3061 cgccaaggac tgcggaccat acgcatcact ccacgagtta agggacaagt tgcgtgacca
     3121 aatggaaaag gcgctcaaga aggacatcga ggagtcctac aaagattccc ttctcaaacg
     3181 cctcatcgat acccatcatt tcgatctccc tgacacactt gtggaacgag agcttagtac
     3241 gattgtgcgg cagaaattgc aagcacagca gcgtggaaaa gtcaccgatt cccttcctgc
     3301 acctgatgcc gaagaactga agcgaattcg cgaagagcat cgtcaggagg caaatcgtcg
     3361 cgtgaaagcc ggcttgattc ttgaggccat cgccgagaaa gaaggtttgt cggtgagcca
     3421 agacgatctg aacaatgaag tgactcgatt agctgcagaa cttagggtac cgatggccga
     3481 tctcgtaaag atgatccagg caggtggtca ggattccgtt gaggaattac gtgcgagaat
     3541 cttggcagac aaggcgctgg acgttgttta tcgtcaagcg gtcattcaag gataagcgcg
     3601 gccattgttg cctcgaattg taaccgccgg aagttgtcca ggaaaggaat tgagcaatta
     3661 tgttggttcc gatcgtagtc gagcagacta atcgaggcga acgagcctat gacatctatt
     3721 cacgccttct caaagatcgc atcattttcc tcggagcccc aatcgacgat gtttttgcca
     3781 acttggtcat tgcacagctt ctctttcttg aagcggagga tcccgagaaa gatattaatc
     3841 tttacgtcaa ttcgccggga gggagtgtga cggccggatt gggcatctac gataccatgc
     3901 aatatgtgaa gcccccgatt aacaccatct gcctcggcca ggctgccagt atgggagcgc
     3961 tcctattgac cgctggcaca aaaggaaagc ggtttgccct gcctaatgcc cgggtgatga
     4021 tccaccaacc gttgggtgga ttccaagggc aggcgacgga aatagacatc catgctcggg
     4081 agattctcaa gattcgtgaa cgcctcaatg aaatcatggc caagcacact gggcaaccga
     4141 tcgagaagat tgcgcatgac acggaacggg actatttcat gtccggcgaa gaagcgaaac
     4201 ggtatggcct cattgacgag gttattacac gaccacccaa atttatgaaa gcggtagagt
     4261 ccagcgacgg ggtaaaagac gggattaaag gcaagtagcg caggagggta catggccaag
     4321 caagaaaaga tggaccgaca cttgcgatgt tctttctgtg gaaaaagtcg tgatgaggtg
     4381 cgaaaactaa tcgccggacc gacggtgtac atatgtgatg agtgcgtcaa tctttgcaat
     4441 gacatcattg cggaggactg ggaagaagcc aaagaagaaa tttcgtccaa actcaagaaa
     4501 cctgcagaaa tcaagcatca cctcgatcag tacgtgatcg ggcaggagcg ggctaaacgg
     4561 attctgtctg tggccgtgca caatcattac aagcgcatct cttcaaagga aaaagaggtt
     4621 gatgatatcg aactccaaaa gggcaacatc ctcatggtgg gtccgacagg gacggggaag
     4681 accctcttgg ctcagacctt ggccaaatat cttgacgtcc cttttacact tgctgatgcc
     4741 accaccctga cagaggccgg ttatgtgggc gaagatgtgg agaatatcat tctgaagctc
     4801 ttgcaagccg ctgactatga tgtagaacga gctgagcgcg ggatcgtgta catcgatgaa
     4861 atcgataaga tcagtcgaaa gagcgacagc ccgtctatta ctcgggacgt atccggcgaa
     4921 ggtgttcaac aagccttact gaagctgatc gagggcacgg ttgcgaatgt ccccccacag
     4981 ggaggaagaa agcatccgca tcaagaattt attcaggtca ataccggcaa cattttgttc
     5041 atctgtggtg gagcatttgt cggcctagaa cagatcatcg aacagcgtct gaatcgaaaa
     5101 gcgatgggat ttggagctga aattcgcgga agaagcgaca tgcgtttagg tgacctgctg
     5161 gctaaggttc agcccgaaga ttttttgaag tacggcctga taccc
//
LOCUS       DATNDD010000228         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2114925, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000228 DATNDD010000000
VERSION     DATNDD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114925"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VN647_05475"
     CDS             <1..1092
                     /locus_tag="VN647_05475"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HWG96513.1"
                     /translation="YSPRLLRNFMRDRGTQFVLSVFVGAFAYSTAGLHTVGVQNPDEA
                     AFMPRLAVSGSLGLALASLGVLIYFIHHLSSSIQIDTIMSMIVRETLEVIDDLYPDQT
                     DFSKSEELSPDPPAWAATLPSDRSGYIQEVTPETLIEVAARNDLVIRFVKSVGHHVVA
                     GTPIACAWHPSADHLPPEPWLQEAIRDSVQIGFERTMLQDVPFGIRRLVDIGNRALSS
                     AINDPYTATQAVHHLSEVLCVLARRRLDDRLYRDDHGTMRVAIPFPGFSDYLQLGTGQ
                     LRRFGAKEPAVARSLIQLLKNVCGSTTSEDRRMVATKHIRLVMEEAKREITQPADIEN
                     LLTDANEALSMLEAGRPQMAHGSTHDWTL"
     gene            1425..2195
                     /locus_tag="VN647_05480"
     CDS             1425..2195
                     /locus_tag="VN647_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96514.1"
                     /translation="MSEGNYMESCRSKGVPIPPDWKQSSSEWESHGNLKTILLTPNNL
                     DQTPVDQTSFARVWSYASPDVRGACIALGRSGGSFQIICQSATTGYVCFWGNDSRSPS
                     TGWTPETAEVPIASLRDPVQGFSPGTVPCTECHRGNNAFLYAPDDPTWATVLRPEHAR
                     PTFTTRVEQSSHHGQLTFGATTITYPRFLPIGGKSMALNNPLSTMPGCSGSCHELHFE
                     ILKRNHTVEGYVRVPRPMGPHCAANSPPDDPTRNCYQR"
BASE COUNT          567 a          803 c          697 g          612 t
ORIGIN      
        1 tattcacccc ggctgctgcg caatttcatg cgcgaccgcg ggacacagtt cgttctgagc
       61 gtcttcgtgg gcgccttcgc ttacagcacg gcaggtttac ataccgtcgg cgtccaaaat
      121 cccgatgagg cggcgttcat gccccgtctg gccgtctccg gatcactcgg actcgctttg
      181 gcaagcttgg gtgtgctcat ttacttcatc caccaccttt cctcctcgat tcagatcgac
      241 acgattatga gcatgatcgt gcgtgagacc ctggaagtca tcgacgacct ttacccagac
      301 cagaccgact tttcgaaatc cgaggaactg agtccggacc cacccgcatg ggccgccact
      361 ctaccatccg accgttccgg ctacatccag gaggtcacgc ctgagacgct catcgaggtt
      421 gcggcacgaa atgacttggt catccggttc gtcaagtcgg tgggtcacca cgttgtcgcc
      481 gggaccccga tagcttgcgc ctggcacccg tctgccgatc atctgccacc cgagccttgg
      541 ctgcaggagg caatccgcga ctcggttcag attgggttcg agcggaccat gctacaggat
      601 gttcccttcg gcattcgccg cttggttgat atcggcaaca gagccctgtc ttcggcgatc
      661 aacgacccct acaccgcaac ccaggccgtg caccacttgt cggaggtcct ctgtgtgctg
      721 gctcgccgac ggttggatga ccggctgtac cgtgacgacc atggcacgat gcgtgtggcg
      781 attccgttcc ctggtttttc ggactaccta cagcttggca cggggcaact ccgtcggttc
      841 ggggccaagg agccggctgt tgcccgaagc ctgatccagc tgcttaagaa cgtgtgcggt
      901 agcaccacga gcgaggatcg gcgtatggtt gccaccaagc acattcggct ggtgatggag
      961 gaagctaaac gtgagatcac ccagccggca gacatagaga acctgctgac ggacgcaaat
     1021 gaggctttga gcatgctcga agcgggccgt cctcagatgg ctcatggctc aacacacgat
     1081 tggactttgt agagctgtgc attgcgcaat gatttctcat gtggacggtg agtctcgtcc
     1141 gaaaacactc tcggtacatt ttctcctatc tgacgaaatc tatggtccgg taaaggaatc
     1201 ttgacatcct cccttgcaat gtataatcgc gatctgatca cataaagcca tatcggaagt
     1261 gcgggaatgg gtactttggg ttttgtgaaa tagtgatatt acttaggaca tctgtcgtat
     1321 ctttggcttg cttgcttctc gcaggctgct cgctgatcga atcgccgaag gaaggaacgg
     1381 agttccgatc ccctattccc gctcaaatag ctgatgtgaa catcatgagc gaaggcaact
     1441 acatggaatc atgtcgttcg aaaggtgtcc cgattccacc tgattggaaa cagtcttcat
     1501 ccgaatggga aagtcacggc aacctcaaga ccattttgct gacgcccaat aatctagacc
     1561 agactccggt cgatcagacc tcgttcgccc gcgtctggtc ctacgcctcg ccggacgtga
     1621 gaggcgcctg cattgcgctg ggaagaagcg gcggatcatt tcaaatcatc tgtcagagcg
     1681 ccacgacggg gtacgtctgt ttttggggaa acgattcacg ctcgccaagt acaggctgga
     1741 cccctgagac agccgaagta cccatcgcct cactccgcga tcccgttcaa gggttttctc
     1801 ctggcaccgt gccctgcaca gaatgccatc gcgggaacaa tgcattcctc tatgcccctg
     1861 atgatcccac ctgggccacg gtcttgagac cggagcatgc gcgcccgaca ttcactactc
     1921 gagtcgagca gtcgtcacat cacggacaac ttacgttcgg cgcgacaacc atcacctacc
     1981 cgcgctttct tcccatcggt gggaaatcca tggctctgaa caatcccctg tcgactatgc
     2041 ctgggtgttc aggatcctgt catgagttgc attttgagat cctaaaaagg aatcatacag
     2101 ttgaagggta tgtcagagtc cctcgaccca tgggccccca ctgcgcggcc aactccccgc
     2161 cggatgaccc gacaaggaat tgctatcaac gctaatcgtc actccctcat gacctcaact
     2221 cagaaactag tagtgaccaa gaccctcacc aataaattgg tatcctaaag ccacatcgcg
     2281 cggtggcgtc tgtctgctgg cgtattgagt gacaaggtcg aacctgctca cctgggcgat
     2341 gcacagttca agatcctacc ccaacttgca cgactgataa tccgtttgcc gtgagcttgc
     2401 gagggcagct cgcaattggc ctcaattgcg tagtgctccg tagtgatctt gacaagcggg
     2461 tatactacgt gctaccgggt gctcctccgc aaagccgtac cagcgatttt tcaggtggag
     2521 ggcgcaatgc agcctgacac cagtcggcga gtcaccaacc gcgtccctct cgttacactc
     2581 acgtctttcc gcgcacctgt ccgatcgcat ccagtcatgg aggcgactcc ggtcttactc
     2641 tctaacattc caagcgtatt cctgactttg acgctgctt
//
LOCUS       DATNDD010000229         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3755060, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000229 DATNDD010000000
VERSION     DATNDD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755060"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..455)
                     /locus_tag="VN647_05485"
     CDS             complement(18..455)
                     /locus_tag="VN647_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HWG96515.1"
                     /translation="MSHAFHFLDDVATADLAFDASGDSLQELFQGATCAVIEALADPA
                     TVGTTWRQTIERADEDLAELLFDWLSELVYWKDAAGVVFNRSELTLARQDHGCWKMTG
                     LLYGEPVNGSAQTLRADVKGVTKHLYRLRQEGGRWIIRVVLDV"
     gene            complement(525..1673)
                     /locus_tag="VN647_05490"
     CDS             complement(525..1673)
                     /locus_tag="VN647_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWG96516.1"
                     /translation="MKRSAISQPEAATLFIAASEHDSNLYYATRFMAPDPFIYLEIKG
                     ERLMIMSDLEMDRAKTQASVDRVLSYSEIEQKAKKQGIKNPATVDVVHVVLKEAKVRR
                     LLVPANFPFIYATRLQELGYSLNPKRDPFYEQRVVKNAEEVRWIELSQRATEDAVALA
                     HDLLRRATIQDGELWCDGVVLTSERVKQLINVKLMEQNCVAQHTIVAGGEQACDPHNE
                     GSGPLPAHRSIIFDVFPRSATSRYFADMSRTVIRGTASPELRRLYGVVKDAQEDAIDK
                     IKDGADGIKIHQGICARFEKAGYKTGLVDGRMQGYFHGTGHGVGLDIHEAPRISRTGS
                     LLQEGHVVTVEPGLYYPGLGAVRIEDMVLVTKDGCRNLTNFPKVFELG"
     gene            complement(1780..>2431)
                     /locus_tag="VN647_05495"
     CDS             complement(1780..>2431)
                     /locus_tag="VN647_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HWG96517.1"
                     /translation="GEVRFSGVLRGHGRNPHGVYPSLNGRVEVLLENGRILKSNERAV
                     WKIISLLNLPAVLQGKVDLEKEGLPYNRISATVAIQDGMFQTENLIIDSPILKITAAG
                     NYDLPTDQLDLAVAVSPFGSYSQFLKTIPLFGRIVAGDRKGIATAMFTMKGAMEDPEV
                     TYLPVKSFASGLSGLAQLAVDVLTNTLTLPIDLVTPNEESEVRPKDMTPSPAPAVP"
BASE COUNT          524 a          711 c          630 g          566 t
ORIGIN      
        1 acagagagtt tgcatcctta tacatccaat accaccctga taatccaccg tcccccttct
       61 tggcgcagtc gatagagatg tttggtcact cccttcacat ccgccctcaa agtctgtgcc
      121 gagccattga ccggctctcc gtacaataac cccgtcattt tccaacagcc gtggtcctgg
      181 cgggccagcg tgagttccga ccggttaaat actactcctg ctgcatcctt ccagtacacg
      241 agctccgaga gccaatcgaa caacagttca gcaaggtcct catctgctcg ttcaatcgtt
      301 tgccgccaag tggtccccac tgttgcagga tcggccaagg cttcgatgac cgcgcatgtc
      361 gccccttgga agagttcctg gagtgaatcg ccggaggcgt cgaacgccag atcagcggtg
      421 gcgacgtcgt ccagaaagtg aaatgcgtgg gacattcttg tgccaaagcg gaatggtcag
      481 ccgattggaa aacgtggaga tgaggggcct gatcagaccg ccgtctatcc gagctcgaag
      541 actttgggga aattggtgag attacgacag ccatccttcg tgaccaacac catgtcctcg
      601 attcggacgg ctcccaatcc tgggtaatac agccctggct cgaccgtgac gacgtggccc
      661 tcttggagaa gtgagccggt tcgactgata cgcggcgctt cgtgaatatc cagccccact
      721 ccatgtccgg ttccgtgaaa atacccctgc atgcggccgt ccaccaagcc ggtcttgtat
      781 cctgcctttt cgaaacgcgc acagatacct tgatggatct ttattccgtc agcgccgtct
      841 ttgatcttgt cgatggcatc ttcttgggca tccttgacga cgccatacaa ccgtcttagt
      901 tccgggcttg ccgtcccgcg tatcactgta cgggacatgt cggcaaaata gcggcttgtc
      961 gccgagcgag ggaacacatc gaagatgata ctacgatgag ccggcagtgg tccgctccct
     1021 tcattgtgag gatcacaggc ctgttcccca cctgccacaa tcgtatgctg ggccacacaa
     1081 ttctgctcca tgagtttgac attgatcaac tgcttgaccc gctcagatgt caacaccact
     1141 ccgtcacacc acaactcccc gtcctgaatg gtcgcccgtc gcaacagatc atgcgccaag
     1201 gccaccgcat cttcagtggc tcgttgggag agctcgatcc atcggacttc ctccgcgttt
     1261 ttcacgacac gttgttcgta gaaaggatcg cgtttcgggt tgagactgta acccagttcc
     1321 tgtaatcgcg tggcgtagat gaaggggaaa ttcgccggga ccagcaggcg tctgactttg
     1381 gcctctttca agacgacgtg cacgacatcg accgtcgccg ggttcttgat cccctgtttc
     1441 ttcgccttct gttcgatttc agagtaggag agcactcgat ccaccgaggc ttgggtcttg
     1501 gcacgatcca tttccagatc gctcatgatc atcagccgct cacctttgat ttcgagatag
     1561 atgaacgggt ccggtgccat gaagcgcgtg gcataataca aattcgagtc atgctcgctg
     1621 gcggcgatga acagtgtggc cgcttccggt tgagatatcg cactgcgttt catgaattta
     1681 ccgtggggta gcaggattga atggaatcct tagagatgtc cagagccgaa cttcaacgat
     1741 gctaacatgg gcatagaggg cggtcaagaa tcatgctggt cacggcacag caggagccgg
     1801 cgagggagtc atatcttttg gccgcacttc ggattcttca ttcggggtca ccaaatctat
     1861 cggcagcgtc agggtattcg ttaagacatc gaccgcgagt tgagccaacc ccgataggcc
     1921 ggacgcaaag gacttcaccg gcagataggt cacttctgga tcctccatcg cccccttcat
     1981 ggtgaacatc gccgtggcga tccccttgcg atcgcccgcc acgattcgcc cgaagagtgg
     2041 aatcgtcttg aggaattgtg agtatgaccc gaacggactc acggcgacgg caagatccag
     2101 ctggtctgtg ggcaagtcgt aattcccggc ggccgtaatt tttagaatcg ggctgtcgat
     2161 gatcagattt tccgtctgaa acatcccatc ttgaatggcg acggtcgcgg aaatcctgtt
     2221 gtatggcagc ccctcctttt caagatcgac cttcccctgc aacacagccg gaagattcag
     2281 caaactaatg atcttccata ctgcccgctc gttcgatttc aggatgcgac cattttccag
     2341 caacacctcg actctcccgt ttaatgaagg gtagacccca tggggattgc gcccatgacc
     2401 gcgaagaacg ccgctgaagc ggacttcccc t
//
LOCUS       DATNDD010000230         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1934684, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000230 DATNDD010000000
VERSION     DATNDD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934684"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VN647_05500"
     CDS             <1..923
                     /locus_tag="VN647_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96518.1"
                     /translation="LLRRTDAASVLEIGAGDLSFAEELVDLYGSELNRQHRPFIVHCL
                     DRLDPRSQLGGPLHASPERLQKLQQKDGLSFSFFGNQDMLGLGDLDQQGVLAPRYTIA
                     TCWAPATPTFAYEPTRLSETFIRGELERTKGAFHRTRFGKEQALEVRHAGRALLFPPW
                     KFEIIGPLALLNLLVRRGFLCVLGSVDAQVFWELLAQLLEDPRYRPPDQLFDSVTLPK
                     VFGEVYHILAGLPIGESIDLAEVASLRRHYLHLPPDSSAFMDSIGGHFRYVRISRGAT
                     FPDTPASSTARKFASMTEEVPPWLLTLVPA"
     gene            1071..2012
                     /locus_tag="VN647_05505"
     CDS             1071..2012
                     /locus_tag="VN647_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWG96519.1"
                     /translation="MNPTDIIRAIQENIARVIKGKPHVIEMSLVALLARGHLLLEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGVSIFNRQKQAFEFMPGPIFANIV
                     LADEINRTTPKTQSSLLEAMSEAQISFDNKTYPLNQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPSPEEEKRVLDRPQSLHPAEELQPLLTAHDVLLLQEQVDHVLMEE
                     SITDYLLAIVQSTRQSDLLSLGVSTRGALALSRAAKALSLVRGRTYCLPDDIKELAPI
                     VLSHRIMVARAQGLRQRSFEQAERIIQDLVDSIPVPV"
     gene            2055..>3153
                     /locus_tag="VN647_05510"
     CDS             2055..>3153
                     /locus_tag="VN647_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWG96520.1"
                     /translation="MQRQSPSFIRRLFRHRSTSVTSEGVQFLFFTLAIGIAAVNTGNN
                     LFYLLLAMMLSLILTSGIVAEYCLRRLEFHRHLPDHLIVNEPTVAMLVVANRKSRLPS
                     FSLKLFDMSDGRELDRGLEIPQLLPGGSRIVPYSLVATRRGRLQLDGVCVGTDFPFGL
                     FKKQTFYPLEETIIVCPEIKPIDERLVRGLLTTGPEQSVDRRGHGNDLYNLRLYQAGD
                     DSRKIHWPTTARTAQLTVRETEAEDQRRAIVYLPTVAPVSHDALFEQAVSLTASIIQH
                     LAHRGYSLQLLVGSSHSSFGQGDLHLWEFLRMLALCERRPPSAETIMPGELPRGSWEV
                     EGGMMIVVQSWMGPGDIEPQHPTILINGNIIT"
BASE COUNT          659 a          927 c          845 g          722 t
ORIGIN      
        1 tcctccttcg tcgcactgac gcggcatccg tgttggagat cggcgccggc gatctctcat
       61 tcgccgagga gttagtcgat ctgtacggct ccgagctcaa tcgacaacac cggccgttca
      121 tcgttcactg cctggaccgg ctcgatcccc gttcccaact gggcggtccg ctccatgcga
      181 gtccggaaag actgcagaaa ttacagcaga aggacgggct atctttctct ttcttcggga
      241 atcaggacat gcttggacta ggagatctgg atcaacaggg ggtgctggcc ccgcgatata
      301 ccatcgccac ctgttgggcg ccggccacac cgaccttcgc ctacgagccg accaggctct
      361 cagagacatt cattcgtggc gaactggagc gcaccaaagg cgcatttcat cggacacgtt
      421 ttggcaaaga acaagcgctt gaagtccggc atgccggccg cgccctcctc ttcccaccgt
      481 ggaagtttga gatcatcgga ccccttgccc tcttgaacct gctcgtccgt cggggattcc
      541 tgtgtgtgct gggatcagtg gatgcacagg tgttttggga actcctggcg cagttgctgg
      601 aggacccgcg ctatcggcca ccggaccaac tctttgactc cgtcaccctc cccaaggtct
      661 tcggcgaggt ctatcatatt cttgccggtc tgccgatcgg cgaatcaatt gatttggctg
      721 aggtggcatc ccttcggcgt cattatcttc atcttccgcc ggactcatcc gccttcatgg
      781 atagcattgg cggtcatttt cggtatgtcc gcatcagtag aggtgccacc ttcccggaca
      841 ctccggcgag cagtaccgca cggaagtttg cttccatgac cgaagaagtt cccccatggc
      901 ttctcacact ggttcccgca taagctgatc cccagctccg tggcttaatg ccgggtcata
      961 gagccgcaaa gcgcttgaat aattgaccgt tcccagaccc tttgttagac ttcaaccgtg
     1021 tcggatcacc agggcattct gcagcggcat cacacaccgt agtcctcatc atgaatccaa
     1081 cggacatcat tcgagccatc caggagaata ttgcgcgcgt gatcaagggt aaaccgcatg
     1141 tgatcgaaat gagcctggtc gcactcttgg cgcgcggaca tctcctcctc gaagacgtac
     1201 cgggagtcgg aaaaacgacg ctcgcgcata gcctcgcgcg atcgctcgat tgctccttca
     1261 agcgcattca gttcacgagc gatctgctgc cgtccgatat cgtgggggtc tcgatattca
     1321 atcggcagaa acaagccttt gaattcatgc cgggtcccat tttcgccaac atcgtgcttg
     1381 cggatgaaat caaccgaacg acaccgaaga cacaaagtag tctattggaa gcgatgagcg
     1441 aagcacagat ttcgttcgac aataagacct atcctctgaa tcagcccttc atggtcattg
     1501 cgacgcagaa tccagccgaa tatcacggca cgtttcccct cccggagtcg caactggatc
     1561 ggttcttgat gcggcttcgc atcggctatc cctcgccgga ggaagaaaaa agggttctcg
     1621 accgaccgca atcgctccat ccagccgaag aactccaacc gttgctgacg gctcatgatg
     1681 tccttctgct ccaagaacaa gtcgaccatg ttctcatgga ggaaagtatt acggactatc
     1741 tcttggccat tgtccaaagc accaggcagt cggacctact gtcattgggg gtgagtaccc
     1801 gcggagcgtt ggcgttgagt cgggctgcca aagcgttgtc gctcgtccgc ggccggacct
     1861 attgtctgcc ggacgatatt aaggaattag cccccatcgt gctttctcac cgcatcatgg
     1921 tggcccgcgc gcaagggctg cgtcagcgaa gctttgagca agccgagcgg atcatccagg
     1981 atttggtcga ctcgattccc gtcccagtct aggtcatccc tgaagtctgt gtcccggcgc
     2041 ggagctggtc gtacatgcaa cgtcaatctc cttccttcat taggcgattg tttcgccatc
     2101 ggtcgacgag cgtgacctca gagggcgttc aattcctgtt ctttacattg gcgatcggca
     2161 tcgcagccgt caataccggc aacaatctct tctacctttt gcttgcgatg atgttgagtc
     2221 tgattttgac ttcgggaatt gtggcggagt actgcctgcg gcggttggaa ttccatcgtc
     2281 atcttcccga tcatctcatc gtgaatgaac cgaccgtcgc catgctggtc gtggcgaatc
     2341 ggaagtcacg gctgccgagc ttttccttga agctgttcga tatgagcgac ggtcgtgaac
     2401 tggatcgagg tttagaaatt ccccaactcc ttccaggtgg cagccggata gtgccatatt
     2461 ctctcgtcgc cacgaggcgt ggccgactgc aattggacgg tgtctgcgtc gggacagatt
     2521 ttccgttcgg gctcttcaag aaacaaacct tttacccgct cgaagaaaca atcattgtct
     2581 gccccgagat caagccgatc gacgagcgtc tagtacgggg tcttctcacc acaggaccgg
     2641 aacagagcgt cgatcgacgg gggcatggca atgatttgta caatctgcgc ctgtatcaag
     2701 cgggggatga ctcgcgaaag attcactggc cgacgacggc gcgaacggcc caactgacgg
     2761 tccgggaaac cgaggccgaa gatcaacgcc gagccatcgt gtatttaccg acagtcgctc
     2821 cagtgagcca cgatgccctg tttgaacagg cggtctccct cacggcctcc atcattcagc
     2881 atttggcaca ccgcgggtat agcctgcaat tattggtggg gtcgtctcac tcgtcattcg
     2941 gtcaaggcga cctgcacctc tgggaatttc ttagaatgct cgctctctgc gaacggcgac
     3001 cgccctctgc agagaccatc atgccagggg aactgcccag aggctcctgg gaggtcgaag
     3061 gagggatgat gattgtcgtg cagtcatgga tgggaccggg agatatcgag ccccaacatc
     3121 ccaccattct gattaacgga aacatcatta ctg
//
LOCUS       DATNDD010000231         4354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_28277, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000231 DATNDD010000000
VERSION     DATNDD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28277"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VN647_05515"
     CDS             <1..1182
                     /locus_tag="VN647_05515"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWG96521.1"
                     /translation="SEQPLRLPVQDVYKFDARRIITGRITAGCLKVGDHLVFSPSNKR
                     ANIRTVEAFNIEPQPTEGQAGQSIGMTLDEQIFVERGEIASHQDNLPQVSTAFRANLF
                     WLGKRPLEKGRKYLLRVATKEVDCEVATIHRIIDTMDLAQQQGSSTISKNQVAELTLR
                     TKVPVSFDLSASFEATGRFVLVDEYDIAGGGIITELIHDDQEFLREEARRRDFAWVKG
                     EVTVEDRAQQYGHRAAVVLITGGRHTGKSFLARKLEGRFVADGRHAYLLDGENLRRGL
                     DADLSEEERGQTAEMARRYGEVARLLTDTGLIVVSTTNPFGLAYCEASQAIRTLVHPA
                     PVIAVHMSKSEEEPPPNTDVVLAGPTDFDAATARIVDELKRRGVLAQAIGAKPVFQYS
                     I"
     gene            1369..2103
                     /locus_tag="VN647_05520"
     CDS             1369..2103
                     /locus_tag="VN647_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HWG96522.1"
                     /translation="MKFVCLNCETYMSLEKVEKPEAGSLGVFFACPSCNAKFSMVTNA
                     GETNMMNALGLKLGARAEPAASMEGLRALAGNGQTSPVMTKPTPTEPTVSATTSSPAK
                     AGEKTSGCPFSAMVADMGLTAGGKPGNGTSPGSSEFTWTADAKEKLDRLPAFVKPMVQ
                     GSVETYARKHGFKTITLQVMDDSKNDSPDGMMWSREAEQRLDNIPDFIRPMARKEIER
                     LAKERGVSTITEQVMDEAKDKFMKFM"
     gene            2198..2953
                     /locus_tag="VN647_05525"
     CDS             2198..2953
                     /locus_tag="VN647_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96523.1"
                     /translation="MKRWAFLLSVLVLMVVQSPWWADVRAQPFSEHYPISVAPGTTNS
                     PHGEHADRGWEGSAQGVAYSEFNHRFAGLFVLLFGLAELGHALRYQLPFWTRLILPSA
                     LAVIGVYLLIWSDHEAWPIGSLSFAQTFFGQDQEIIQHKFYGVFGATAAVSETLRRIG
                     WAQHPAWAAPLVFLGLVGALLLFVHSHGSHPANHTIELHHALLGSFGIGAAISNAMVS
                     WTSGPSNQSVKRWEAAWAGFVIVMGIQLLIYFE"
     gene            3089..3847
                     /locus_tag="VN647_05530"
     CDS             3089..3847
                     /locus_tag="VN647_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWG96524.1"
                     /translation="MGGHSHWATIKRHKGAQDAKRGKIFTRIIRELTIAARSGGDPDG
                     NPRLRLAIAKAKEANMPGDTMKKAVQRGTGELPGVTYEEFSLEGYGPGGTALLLEITS
                     DNRNRTVAEIRSLLTKNHGNMSEAGAVAWQFHKKGLVAIEKGKVDEDTLLSLALDAGA
                     EDVKVGEKSYEVFTSPQDFEAVKKALVDAKIETTLAEITYLPQNTIRLEEKSAEQMLK
                     LMEVLDEHDDVQKVHANFDIPDEVMEKVAATAAG"
     gene            3902..>4354
                     /gene="ruvA"
                     /locus_tag="VN647_05535"
     CDS             3902..>4354
                     /gene="ruvA"
                     /locus_tag="VN647_05535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWG96525.1"
                     /translation="MIAFLTGRLAFKAPTHLTLDVQGVGYEVHIPLSTYYALPNLDEV
                     AALNIHTHLREDAIQLFGFLSQSEKESFLILTGVSGIGPKLALSVLSSLSVTDLVHAI
                     QSEDIEKLATVPGIGKKSAGRIALELKDKVDKIHGVRPQAAVADAPELD"
BASE COUNT          977 a         1153 c         1276 g          948 t
ORIGIN      
        1 tcggagcagc cccttcgact gcccgtgcag gacgtctata agttcgatgc acgccgaatc
       61 atcaccggcc gtatcacagc cggatgtcta aaggtgggag atcaccttgt cttctcgccg
      121 tccaataagc gggccaatat caggacggtc gaggccttca atatcgagcc gcaaccgacc
      181 gaaggccagg cggggcaatc gatcgggatg acgctcgacg aacaaatctt tgtcgagcgt
      241 ggcgaaatcg cctcgcatca ggacaatctt ccgcaggtct cgacggcctt ccgggccaat
      301 ctgttctggc tcggcaagag gccgctggaa aaagggcgca aatatctcct gcgtgtggcg
      361 accaaagaag tggactgcga ggtggcaacc atccatcgga tcatcgacac gatggatctc
      421 gcccagcaac agggcagcag taccatcagc aagaaccaag tggccgagct aaccttgcgc
      481 acaaaagtcc cggtctcatt tgacctatcg gcatcgttcg aggcaaccgg ccgcttcgtc
      541 ctggtggacg aatacgacat cgcaggcggc gggatcatca cagaactgat ccatgacgac
      601 caggaatttc tccgtgaaga agccaggcgg cgcgactttg catgggtgaa gggcgaagtc
      661 acggttgaag accgtgcgca acagtacggc catcgggcgg cagtcgttct gattaccgga
      721 ggacggcaca ccggtaagtc gttcttggcc agaaagctcg aaggacgctt cgtggcggat
      781 gggcgccatg cctacttgtt ggatggcgag aatctgcgcc gtggactcga tgccgatctc
      841 agtgaagaag agcgggggca gacggcagaa atggcacggc gctatggcga agtggcgcgt
      901 ctccttaccg acacaggtct catcgtcgtc tccacgacta atcccttcgg cctggcctat
      961 tgcgaggcct cacaagcgat caggaccctc gtgcatcccg cgcccgtcat cgccgtccac
     1021 atgagcaagt ccgaagaaga gccaccaccg aataccgatg tcgtcctcgc aggtcccacg
     1081 gatttcgacg cagcgaccgc tcgtatcgtc gacgaattaa aacgccgtgg cgtcttggct
     1141 caggccatcg gggccaagcc ggtctttcag tattcgatct agagtcgatt atctgttcga
     1201 gcagatggac actgcaacag tcacataata tttgaaactc atgcctcccg agttttagag
     1261 ccccgtgcct aactccctcc gcccctccag ccgtttgact caccgaaaat ccttgtgata
     1321 gcgtaggctt gaagcaaatt ttcggggatc gatggaacag gagtctttat gaaatttgta
     1381 tgtcttaact gcgaaaccta tatgagtctc gagaaggttg agaagccgga agcgggttcg
     1441 ctcggagtct tttttgcctg cccttcgtgc aacgcgaagt tctcgatggt gacgaatgcc
     1501 ggcgaaacga acatgatgaa tgcgttgggg ttgaagctgg gagcgcgggc tgagccggcg
     1561 gcctctatgg aggggttgcg agcgcttgca ggaaatggtc aaacatctcc tgtgatgacc
     1621 aagccgactc cgactgagcc gaccgtatcc gctacgactt cgtcgcccgc taaggctggg
     1681 gagaagacga gtggttgccc gttctcggcg atggtagctg acatggggct gacggccggc
     1741 ggcaagccag gaaacggtac gagtccgggt tcctccgagt ttacctggac tgccgatgcc
     1801 aaagagaagc ttgaccggct accggcattc gtgaagccga tggtccaagg gagcgtcgaa
     1861 acttacgcgc gcaagcatgg ttttaagacc atcacgctgc aagtgatgga cgattcaaag
     1921 aatgattcgc ccgatggcat gatgtggtca cgcgaggcgg aacaacggct tgacaatatc
     1981 cccgacttca tccgtcccat ggcccgaaaa gagatcgagc gtctggcgaa ggaacgagga
     2041 gtgtcgacca tcactgagca ggtgatggac gaggcgaagg acaagttcat gaagttcatg
     2101 tagttgagag ctccatgaat cagatgtcgt gaaggcccat ccggcctccc ggatgggcct
     2161 tctgagtgtt tcggctcatt cgtttgacag ttctcgcatg aagcggtggg cttttctact
     2221 gtctgttctg gtactgatgg tcgtgcagtc tccctggtgg gcggatgtgc gcgcccaacc
     2281 attctcagag cattatccaa tttctgtcgc tcctggtaca acgaacagtc cgcacggtga
     2341 acatgccgat agaggatggg aagggtctgc tcaaggagtc gcctattcgg agtttaacca
     2401 tcgcttcgct gggttatttg tgctgctgtt tggcctggct gagttaggac atgcattgcg
     2461 gtatcagctg cctttctgga ctcgtcttat tcttccaagt gcgctcgcgg tcatcggagt
     2521 ctatctgttg atttggagtg atcatgaggc ctggccgatc ggttcactca gctttgccca
     2581 gaccttcttt ggacaagacc aggaaatcat ccagcataaa ttctatggcg tgtttggcgc
     2641 cactgcggcg gtgagcgaga cgcttcgccg aatcggctgg gctcagcacc cggcctgggc
     2701 ggctccattg gtgtttcttg gtctggtcgg tgcattgctg ctgtttgttc actcgcatgg
     2761 gagtcatcca gcgaatcaca cgatcgagct gcaccatgca cttctcggaa gtttcgggat
     2821 tggggctgcc atatccaacg ccatggtctc ttggacatcc ggcccctcga atcagtctgt
     2881 gaagagatgg gaagcggctt gggcgggatt cgtgattgtg atgggtattc aactcctcat
     2941 atactttgaa taaataaact cgtccgctac cgggcgtaat tgactccttt ctgaagcctg
     3001 tgttacgtct aatcattcac aggcatgctg ccttagtaca tatctctaac tagctcaaag
     3061 agtttgggct ggggtagagg aggaacatat gggtggacat agtcactggg ccacgatcaa
     3121 acggcacaag ggagcccagg acgcgaagcg cggaaagatc ttcacgcgaa tcatccggga
     3181 gcttaccatt gcggctcggt ccggtggtga ccccgacggg aatccccggc tgcgtctggc
     3241 gatcgccaag gccaaggagg ccaatatgcc tggcgacacc atgaagaaag ccgtccagcg
     3301 gggtaccggc gaactacctg gtgtgaccta tgaggaattt tctttagagg gatatgggcc
     3361 cggcgggacg gcacttctgc tggaaatcac cagcgacaat cggaaccgga cagttgcgga
     3421 gattcgcagt cttctgacga aaaatcacgg caatatgtcg gaagcgggcg ccgtcgcttg
     3481 gcagttccac aagaaggggc tcgtcgcgat tgagaaggga aaggttgatg aggatactct
     3541 tctttcgttg gctctcgacg caggagccga agacgtgaag gtcggtgaga agagttatga
     3601 ggtgttcacc agtcctcaag attttgaagc ggtgaagaaa gccttagtcg atgccaagat
     3661 cgaaacgacg cttgcggaaa ttacgtacct gcctcagaat accatcaggc tggaagagaa
     3721 gtctgccgag caaatgctga agctgatgga agtcttggac gagcatgatg acgtccagaa
     3781 ggtccacgca aacttcgata ttccggatga ggtgatggag aaagtcgccg cgacagcggc
     3841 gggataatca tcagcttacc accatggttc ttgggttctt gccctcggat aacgaaacga
     3901 catgatcgcc tttctcacgg ggcggttggc attcaaggca cccacccacc tcacgctcga
     3961 tgtgcaggga gtggggtacg aagttcatat cccactcagc acctattacg ccctgccgaa
     4021 tctcgacgaa gttgccgcgc tgaatattca tacgcatctc cgtgaagatg ccattcagct
     4081 cttcggcttt ctctcgcaaa gcgagaagga gtcgtttttg attctgaccg gcgtgtcggg
     4141 catcggcccg aagctcgccc tcagtgtgct ttcaagcctg tcggtcaccg atcttgttca
     4201 tgcgatccag tctgaggata tcgaaaaact tgccaccgtt cccgggatcg ggaagaaatc
     4261 ggcgggacgt attgcgcttg aactgaaaga caaggtggac aaaatccacg gtgttcgtcc
     4321 ccaagccgct gtcgctgacg ccccagagct ggat
//
LOCUS       DATNDD010000232         7126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3502833, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000232 DATNDD010000000
VERSION     DATNDD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502833"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VN647_05540"
     CDS             complement(<1..502)
                     /locus_tag="VN647_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWG96526.1"
                     /translation="MRILITGAGGQLGSALCEVFGYETVIPKDLPDFDLTRPTVEEEI
                     IGSAPDLIIHAGAYTDVDGAEREPDLALAVNAKGTEQIVRAAVRLGARLIYISTDYVF
                     DGEQRVPYREDDSPRPINRYGFSKWKGEQAVLASGARALVIRTAWLYGPVGKNFVKAI
                     MGAAQTE"
     gene            complement(552..1568)
                     /gene="rfbB"
                     /locus_tag="VN647_05545"
     CDS             complement(552..1568)
                     /gene="rfbB"
                     /locus_tag="VN647_05545"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWG96527.1"
                     /translation="MRILVTGGAGFIGSHLVRRLLASAQHQIVNLDALCYSGNLANLD
                     RISRQPGYEFIHGDICDGSLVSSLLRKYRIEGIINCAAETHVDRSILDPGSFARTDVV
                     GTGVLLEEGRHAGVKRFLQVSTDEVYGTVESGLSTEEDRVEPRSPYSASKAGADLLVL
                     SYWTTYQFPVVVTRGSNTYGSHQYPEKFIPLFVTNAIDDQLLPLYGDGKYRRDWLAVE
                     DHCAGIEQAFFHGEPGALYNIGGGNERENIVVAEEILSYLGKPKSLLRFVADRPGHDR
                     RYAVDSGKLRRLGWRPVVPFEEGLRATVQWYRDHESWWRPIKSGEFRQYYEQLYGTRL
                     REAG"
     gene            complement(1569..2309)
                     /locus_tag="VN647_05550"
     CDS             complement(1569..2309)
                     /locus_tag="VN647_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWG96528.1"
                     /translation="MKGVVLAGGLGTRLLPLTKVTNKHLLPVYNRPMIYYPIQTLVDA
                     GVTEIMLVTGGNNAGDFLKLLGNGKEFGLKHLNYTYQEGEGGIADALRLAEHFADGEP
                     ICVVLGDNIIQGSVAKAADHFRRQQRGAKILLKEVKDPQRFGVPVLEGERVVTIEEKP
                     LHPRSPYAVTGIYFYDPHVFEFIRSLKPSARGELEITDVNNAYLKAGILTWDLLQGWW
                     TDAGTIESLYLANQLVGQTGANNVSLAA"
     gene            complement(2426..4321)
                     /locus_tag="VN647_05555"
     CDS             complement(2426..4321)
                     /locus_tag="VN647_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HWG96529.1"
                     /translation="MKQAVVKLFHSVAGRYGGTVFGHRTTLVIGVQLALILAANLTAF
                     ALRFEGDIPPEYEQIMLDHVPAVLLIFGAGLWVFGIQRGLWRYVGLHDLGKILLASLS
                     SAAVFYGVIHLIGGITQYPRSVIILTGLLNGLYLAGIRLAVRGFREWIQVVGPRARRV
                     LIVGAGHAGELLVRDMLADANYDCRPVGFVDDDPIKQRKRIHGIPVVGTIADTKKAAD
                     RLDAQEIIVAIPSSSTAVKQKILAASEGCMVPIKTLPNVKRLLGDSVLLQQVRPMNLD
                     DLLQREPIQTDRQELPPFITGKTLLVTGAGGSIGSELCRQIARHKPQALVLFERYENA
                     LHSLLLELRAAFSEVKLFPVIGDVTVLDRVSEVFHQTSPDIVFHAAAHKHVPLMELNP
                     KEAIRNNILGTRVVAEVALKTGVDRFVLISTDKAVNPSSVMGVTKRIAEHLVREFNHT
                     GLTKFTVVRFGNVLGSNGSVVPLFSEQIRKGGPVTVTHSDIKRFFMTIPEAVQLVLQA
                     SVMGRGVEVFVLDMGEQIKIADLARNMIVLAGLVPGKDVDIVFTGLRPGEKLYEELFE
                     ESEQAEPTTHPKIHRAAGTPVPVGELGGWLESLPATLPKSDEEELLQDLKRLVPSFQP
                     DLSQGVS"
     gene            complement(4318..5418)
                     /locus_tag="VN647_05560"
     CDS             complement(4318..5418)
                     /locus_tag="VN647_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWG96530.1"
                     /translation="MNSALVVCTRRIGDVLLSTPVVRSLKAALPHLMVDMLVFEGTQD
                     IVSANRDIRRIWTIPERPPIGTHLRLLRSIRRRYDVALSVLAGDRPTFHAWMAGRYRI
                     GTLLPDNKSWWKRPLLHEWIPFDNLYTHTVVMNLRLLAPLGIKPLGTPVVSWRDEDEA
                     SVHRRFPGAASHRPYAVLHVSPKFSYKTWSVTGWVALGRWLIDQGLVVGVTGGASDQR
                     AYSEQVVERLPDAVNLVGHLALPAIGYLLSRATVYVGTDTAVSHMAAAVGAPTVVLFG
                     PSNPVKWGPWPKDFPATAQSPWKIHGSQRLENVYLLQGEGDCVPCLAEGCDRHINSLS
                     ECLQQLSVNRVIQAVEMMLAERGRRVRTSGLA"
     gene            complement(5474..6277)
                     /locus_tag="VN647_05565"
     CDS             complement(5474..6277)
                     /locus_tag="VN647_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWG96531.1"
                     /translation="MIVTTYNRPDALAVVLEGYCAQSDHDFGLVVADDGSKGETAEVV
                     RQFARRAPFPLTRVWHEDRGFRAAAIRNRSVASADADYVIFTDGDCVPSRHFVRVHKQ
                     LAEPGYFLGSNRVLLSAGLTNRVLHQQLPIHTWSGIDWIQSWFRREVNRVLPLINLPD
                     APFRKWTPDRWKGIKTCNLSVWRTDLIRVNGLDESYEGWGLEDSDLVIRLLHAGVKHK
                     NARLAATVFHLWHPEQDRMRLPDNQKRLDDLLRSSTVRAAIGLDQHDGL"
     gene            complement(6286..7086)
                     /locus_tag="VN647_05570"
     CDS             complement(6286..7086)
                     /locus_tag="VN647_05570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWG96532.1"
                     /translation="MSISAYIIAFNEARKIADTINSVLWADEIIVADSASTDETARIA
                     QDLGARVVQIPFQGFGHLRNQAVKACTHEWIFSLDTDEQCTAEVRDEVLLILSGIPTH
                     DAYLVPRRNYFMGRWIEHSGWYPNFRQPQLFRKGSMKYVESPVHEGYELLTAKPVGRL
                     QHAIWQIPFRDFEEVVKKANRYSSLGMLKLADHKVTMGTALFHGIWSFLKHYVAKRGF
                     LDGWAGFVIAFGNFEGTFYRYAKRFEQQEMWSLPKQAPLRRRSDSPSK"
BASE COUNT         1606 a         2151 c         1843 g         1526 t
ORIGIN      
        1 gctcggtctg cgcagccccc atgatcgcct tcacaaaatt cttaccgacc ggtccataga
       61 gccatgcggt ccggatgacg agtgccctgg cgccggacgc cagaacggcc tgctctcctt
      121 tccattttga aaatccatat cggttgatcg gacgagggct atcgtcctca cgatagggga
      181 cccgctgctc cccgtcgaac acatagtcgg tagaaatata tatgaggcgg gcgccgagtc
      241 gaacggcggc acgtacgatc tgttccgtcc ccttggcgtt caccgccaac gcgaggtccg
      301 gctctcgttc ggccccatct acatcggtgt aggcccctgc atggatgatc aggtcgggag
      361 ccgatccgat gatctcttct tcaaccgtcg ggcgcgtgag atcaaagtcc ggtaggtcct
      421 tggggataac ggtttcgtac ccaaatacct cgcaaagcgc gctgcccagt tgcccaccgg
      481 ctccagtgat aaggatgcgc atgacgcgaa tgtttaggtt gaggttgacc ttagccttag
      541 cctgcttgga cttaacctgc ttccctcagg cgtgtcccat acaattgttc gtaatattgt
      601 cggaactctc cggacttgat gggacgccac caggactcat ggtctcgata ccattgaacg
      661 gtcgcacgca gcccttcttc gaacggcacg acagggcgcc atcccagccg acggagcttc
      721 ccgctatcaa ccgcataccg ccgatcatga ccaggtcggt ctgccacaaa gcgaagcaag
      781 ctcttcggct tgcctaggta cgagagaatt tcctcggcaa ccacaatgtt ttcgcgctcg
      841 ttacccccgc cgatgttgta cagggccccc ggctctccat gaaaaaatgc ctgttcgatc
      901 ccggcacaat ggtcttcgac ggcaagccaa tcgcggcggt atttgccgtc gccgtacaac
      961 ggtaacagct gatcatcaat ggcattcgtc acgaagagcg gaatgaattt ctccggatac
     1021 tgatgggagc cataggtatt gctgcccctc gtcaccacca ctggaaattg ataggtcgtc
     1081 caatagctta gtacaaggag atctgccccc gctttactgg ccgagtaggg gctacgtggc
     1141 tccactcggt cttcttccgt cgacagaccg gattcgactg tgccgtacac ctcgtccgtg
     1201 ctcacttgaa gaaaccgttt cacaccggca tgtcgccctt cttccagcaa cacgcctgtg
     1261 cccacgacat cggtgcgcgc gaagctcccc gggtcaagaa tcgaacggtc gacatgcgtc
     1321 tccgccgcac aattgatgat gccttcgatt cgatatttgc ggagcaggga tgaaaccaac
     1381 gatccatcgc aaatgtcgcc atggatgaac tcatatccag gctgtcggct tatccggtcc
     1441 aggttggcca gattccccga atagcagaga gcatcaaggt tgacgatctg gtgctgtgcg
     1501 gaggccagca gacgtcggac aaggtgagaa ccgatgaacc cggcgccgcc ggtcacgagg
     1561 atacgcatct atgccgctaa actaacgtta ttagcgccgg tttggccgac cagctgattg
     1621 gccagataga gagactcgat agtgccggca tccgtccacc atccttgaag cagatcccat
     1681 gtcagtattc cggccttgag gtaggcgttg ttgacgtcgg taatttccag ttccccccta
     1741 gccgacggtt tgagcgatcg gatgaactcg aatacgtgtg gatcataaaa ataaatgccc
     1801 gtgacggcgt acggcgatcg aggatggagc ggtttctctt cgatcgttac gacgcgttca
     1861 ccttccaaaa ccggcacgcc gaaccgttgc ggatccttga cttctttgag gagaattttg
     1921 gcccctcttt gctgacgtcg aaagtgatcc gccgccttgg cgacgctccc ttgaatgatg
     1981 ttatcgccca acaccacgca gatgggctcc ccatcggcaa agtgctcagc caaccgaagc
     2041 gcgtcggcaa taccgccttc gccttcctga taggtatagt tgagatgttt gagcccaaac
     2101 tctttcccgt tcccgagcaa ttttaaaaag tcacccgcat tgttccctcc cgtcaccaac
     2161 atgatttccg tcactcccgc atcgaccagc gtctgaatcg gataatagat catcggccgg
     2221 ttataaaccg gaagcaggtg tttgtttgtg accttggtga gaggcaataa cctggtcccc
     2281 agcccgcctg ccagcacaac acctttcact gctgtacctt cctattgcaa gtggttccct
     2341 gttgttttca tcggttgccg ttggttggaa ctgtagggct tgacgataac acgaaggggt
     2401 aaaatcacca ggtgatatgc aaaaactacg aaacgccctg agagaggtcg ggctgaaagc
     2461 tgggcacaag tcgcttgaga tcttgaagca gttcttcttc gtcgcttttg gggagcgtcg
     2521 ccggcaatga ctccaaccat ccgcctaatt cgcccaccgg gacaggggtc cccgcagctc
     2581 gatggatctt cggatgggtg gtcggctcgg cctgctcgct ctcctcgaac agctcctcgt
     2641 acaatttctc tcctggccga agccccgtaa agacgatgtc aacgtctttt ccaggaacga
     2701 ggccggccaa tacgatcatg tttctggcca gatcggcgat tttgatctgt tcccccatat
     2761 cgaggacgaa cacttctact ccccgcccca tcacgcttgc ctggagaacc aattgcacgg
     2821 cctccgggat cgtcataaaa aaccgcttga tgtccgaatg ggtgacggtc accggaccac
     2881 cttttcggat ctgctcggaa aacaggggca ccacgctccc attgctgccc aatacgttgc
     2941 cgaaccgcac gaccgtaaat ttcgtgagac cggtgtggtt gaactcccgc accaaatgct
     3001 ccgcgattct cttggtgacg cccatgacgc tcgacggatt gacggcctta tccgtggaga
     3061 tcagcacaaa acggtccaca cctgttttca acgcgacttc ggcgaccacg cgggtcccta
     3121 ggatattgtt gcggatcgct tccttcgggt tcagttccat cagcggaaca tgtttgtgcg
     3181 cagcagcatg aaatacaatg tcagggctgg tttggtggaa tacttccgat acgcggtcga
     3241 gcacggtcac atccccgatg acagggaaca gctttacttc cgaaaaggcc gctcgtaact
     3301 ccagcaggag cgaatggagg gcattttcat accgttcgaa caaaaccaag gcttgcggct
     3361 tgtgccgggc gatttgcctg cataattccg atccgatcga acctcccgcc ccggtgacga
     3421 gcagcgtctt cccggtgatg aaagggggca gttcttggcg atccgtctgg atcggctcac
     3481 gctgcaacaa atcatccaaa ttcatcggcc tcacttgctg gagcaagacc gaatcgccca
     3541 gtagacgctt gacattcggc aaggtcttaa tcggaaccat gcagccttcg gaggccgcga
     3601 gaattttctg ttttaccgcc gtcgaagacg atggaatggc gacgatgatc tcttgggcat
     3661 ccagcctatc ggccgccttc ttggtatcgg caatcgtccc cacaaccgga atcccatgga
     3721 tccttttcct ctgtttgatc ggatcgtcat ccacgaaccc caccggacgg caatcataat
     3781 ttgcgtcggc caacatgtcc ctgaccaata gctcgcccgc atgtccggca ccgacgatca
     3841 agacgcggcg cgcgcgcggg ccgacgacct ggatccattc ccggaatccc cgcacggcca
     3901 accgaattcc cgccaaatac aatccgttca acagaccggt caggatgatc actgatcgag
     3961 gatactgcgt aatacccccg ataagatgga tcactccata aaacaccgct gcgctgctca
     4021 gagaggccaa aaggattttg cccagatcgt gaagaccgac atatctccaa agccctcgct
     4081 gaatcccaaa tacccataaa ccggcgccga atatcagcaa cacggcgggc acgtgatcca
     4141 acatgatctg ttcatattcg ggaggaatat caccttcaaa gcgcaacgca aaggcggtca
     4201 aattggccgc caggatcaga gccagctgca caccgataac aagggtcgtg cgatggccga
     4261 acaccgtgcc cccataccgt ccagcgacgg agtggaacaa tttgacgacc gcctgtttca
     4321 tgcgagtcct gatgtccgaa cgcgacgccc ccgctccgcc aacatcatct cgacggcttg
     4381 gatcacacga ttcacggaca actgttggag acattcgctt aaactgttga tatggcgatc
     4441 gcagccttcg gccaagcacg gtacgcagtc tccttcccct tgaaggaggt agacattctc
     4501 cagccgttgc gatccatgga ttttccaggg gctttgcgct gtggcaggga agtccttcgg
     4561 ccagggcccc cacttcaccg gattcgatgg cccgaacagg acgacggtcg gcgcaccgac
     4621 ggccgccgcc atgtgactca cagcggtatc cgttccgaca tatacggttg cgcggctgag
     4681 cagatacccg atcgccggca atgccaggtg gccgacgaga ttcaccgcat ccggaagcct
     4741 ctcgaccact tgctcactat aggctcgttg atccgacgcc cctcccgtca ccccgaccac
     4801 aagtccctga tcgattaacc atctcccgag tgcgacccag ccggtcaccg accaggtctt
     4861 gtaggaaaac ttcggcgaga catgcaatac ggcgtatggc cgatgactcg ctgctcccgg
     4921 aaaccggcga tgaacggacg cctcatcctc atctctccag ctgactactg gtgtccccaa
     4981 aggtttgatc cctaacggtg caagcaatcg cagattcata acgaccgtat gggtatataa
     5041 attatcgaac ggaatccatt catgaagcag gggccgtttc caccatgatt tgttgtccgg
     5101 caacaacgtt cctattcgat atcgaccggc catccaagcg tgaaaggtag ggcgatcgcc
     5161 ggccaaaacc gaaagggcga catcatagcg ccgccgaatc gagcggagca atctcagatg
     5221 agtaccgatc ggagggcgtt cgggaatcgt ccagattcgt cgaatatcac gattggcgga
     5281 tacaatatcc tgagtccctt cgaagacaag catatcgacc atcagatgcg gcaacgccgc
     5341 cttgagcgag cgaaccaccg gcgtggacaa gagtacatcc ccgattcgac gcgtacacac
     5401 gaccagtgcg ctgttcattt cccgcttcat ttcgcaatcc ggatttcgtc atcaggacga
     5461 tggccggtcg gcatcagaga ccatcatgtt gatcgagtcc aatggcggct cgaacggtcg
     5521 acgaacgcaa aagatcatcc aggcgtttct gattgtcggg cagcctcatg cgatcctgct
     5581 cgggatgcca caaatgaaat accgtcgcag ccaagcgggc attcttatgt ttgactcctg
     5641 catggagaag ccgaatgact aagtctgaat cctccagtcc ccatccctcg taagactcat
     5701 ccagtccgtt cacacggatc aagtctgtcc gccaaacgga gagattacac gtcttgatgc
     5761 ctttccaacg atccggcgtc cactttcgaa acggagcatc gggcagattg atcagcggaa
     5821 gcacccgatt gacttcgcgc cgaaaccagg actggatcca atcgattccg ctccacgtat
     5881 gaatcggcag ctgctgatgc aggacgcggt tcgtcagccc ggccgagagc aatacacgat
     5941 tcgatccgag gaagtaacct ggttcggcca attgcttatg gacacgcacg aaatggcgcg
     6001 agggaacaca gtcaccgtct gtgaaaatca catagtcggc gtccgccgac gcgacggagc
     6061 ggttacggat cgcggcggct cgaaaccccc ggtcctcgtg ccagacacgc gtcagcggaa
     6121 acggcgcgcg ccgtgcaaat tgcctcacga cctcagccgt ctcccctttc gacccgtcat
     6181 ctgcaaccac gagcccgaaa tcgtgatcgc tttgggcaca atagccttcc agtacgaccg
     6241 ccagcgcatc agggcgattg tatgtcgtca cgatcaccgc tgttttcatt tcgacggtga
     6301 gtcgctgcgg cgccgaagag gcgcctgttt gggaagcgac cacatctctt gctgttcgaa
     6361 ccgcttggca taccgataaa acgttccctc gaaatttccg aaggcaatga caaacccggc
     6421 ccacccgtcc aggaaccctc gcttcgccac ataatgtttg aggaacgacc agatgccgtg
     6481 aaataacgcg gtccccatcg tcactttgtg atcggcgagc ttcagcatcc cgagcgacga
     6541 ataccggttg gcttttttga cgacctcttc aaagtcccta aatggaatct gccagatcgc
     6601 atgctgcaac cggccgaccg gtttggcagt cagtaattcg tacccttcat ggaccgggga
     6661 ttccacatat ttcatagacc cttttcgaaa caattgaggc tgtcggaaat tcggatacca
     6721 gccggaatgt tcgatccagc gtcccatgaa gtaattccgc ctcggcacaa gatacgcatc
     6781 gtgggtggga attccggaaa ggatcaggag aacttcatca cggacctctg cggtgcattg
     6841 ttcgtccgtg tcgaggctga aaatccactc atgcgtgcag gctttgacgg cttgattccg
     6901 cagatggccg aatccctgga acggaatctg gacgacgcgg gcccccaagt cttgtgcaat
     6961 ccgagccgtc tcgtcggtac tggcggaatc ggccacaatg atttcgtcgg cccacagcac
     7021 gctgttgatg gtatcggcga tcttcctcgc ctcgttgaaa gcgatgatat aagccgaaat
     7081 actcatgtcc tcgtaccggc ttgcgaatca atccccgaat acatca
//
LOCUS       DATNDD010000233         9358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_77901, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000233 DATNDD010000000
VERSION     DATNDD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77901"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VN647_05575"
     CDS             <1..1230
                     /locus_tag="VN647_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96533.1"
                     /translation="SDFLWEDFLRRQYSNLLPLLQDYRDAPLMTPRATLRKELDRLVN
                     RAKTDEARKAALNSFKDRELFRIDMKHIVEPGTALPDFSAALTQLAEVIVDRSLKDCQ
                     AKLNKLYGLPRLTNKKPCPFTVLGLGKFGGKELGYASDIEVMFVYGDAGRTGGKQPIE
                     NSEYFERLGTELLQWIEAKQEGIFHLDVRLRPHGGKGSLTNSFDEITGYYSNHGLAAP
                     FERQSLIKLRHVAGDAALGKRVEAHRDGYVYSGKPWNLRIALDLRRQQVKQLVERGTI
                     NLKHSPGGIVDIEYAVQYLQIMHGPRFPILHTPNTMQALAALVDCGIVTRQDGETVRK
                     AYFFIRMLIDGLRMVRGNAKDRVLPPVDSDEFIFLARRVGYTTDDWQAGARHLETDVQ
                     EHMGKVKELFTRTFGKV"
     gene            1312..2031
                     /locus_tag="VN647_05580"
     CDS             1312..2031
                     /locus_tag="VN647_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWG96534.1"
                     /translation="MACYIGLSGYSYKAWQGPDRFYPSGIKPTDFLRYYATKYQTVEL
                     DGVWYRLPIEKTVQAWLIDTPPHFIYAPKVHREITHVHRLKPEGLPMLRAMLQRLEPL
                     HKAGKLGPILIQLPPNLQRDDGRLETFIESLPATHQWAVEFRHESWQADNVEGILRRY
                     AVAWAAVETDEHEAEHRDTTRFRYVRLRKSEYGQAELRRWAERFQNAFQQGQDCFVYC
                     RHEDEGSPWLWADELHRLLNS"
     gene            2308..4557
                     /locus_tag="VN647_05585"
     CDS             2308..4557
                     /locus_tag="VN647_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWG96535.1"
                     /translation="MISDERFTRFIRSGFLLAPLFLLACSSGGGEGSVPSGSPLTPAG
                     PPITWTLKNPMPTPRTEFGVATVNNKIYAIGGYSNSGSVLQTVEEYDPTTDTWTRKAD
                     MPTPRRQLIVVSVNNKIYAIGGVNFSSDPNNLIYSYSTEEYNPATNTWASKASMPTGG
                     AVNNVLGNRFIGGAVANGKIYIAVHSNPGTLSPITHTLEYDPAANMWTSKTQPPFSYT
                     RYTVASLNNKVYALSDIGELAEYDPLKDRWIIRPPLSTPRFRTGLASVGGKLFSIGGG
                     QNSSPVNTVEEYDPETQNWALRASMASPRVSVAVGEALGRLYAIGGSSNTSETFPSPL
                     AAVEEGTPPPPGSVLQIPTGDVVTTGNGQVTVRWNPVAGATSYNLYMASDINLDRSNY
                     AGLPNGAKFVGVTSPHTITGLTNGKTYYLFVTAADVGRESEESFGSSATPTAPPGLLW
                     RTKASMPTPRLEAGAAVVNGKVYVVGGFSGSALATTEEYDPVTDTWTTRADMPTARRS
                     PVVAMANNKIYAIGGMSYIDVNQVTYSYATEEYDPSTNTWTSKTSMPTGNSVNSVLGN
                     RFIAGTAVNGKIYITVFNNGGTPPISSTFEYDPATNTWDTNKSPVPFGNAQYTVASLG
                     GKLYTLTSGSFAEYTPATNTWIIRPSPPQTFSQMRLVADVTKNKLYAIGGYNASDIHD
                     TVQEYDPTSNVWTTQVSVPVPRHSTTTAAIGGKVYVFGGSLVLDFINAAGVPTFIRPL
                     PLSDVEEGQ"
     gene            complement(4702..5559)
                     /locus_tag="VN647_05590"
     CDS             complement(4702..5559)
                     /locus_tag="VN647_05590"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWG96536.1"
                     /translation="MPLASELVQSCTTVFTLPEIYSRVREVVDDPDSTMDDLADVLKL
                     DPAISARLLCIVNSPLYGFPKQIDTISRAVNIVGMQAINDLVTATTVGRTFSGMPIQF
                     MDVTMFWRKSVLCALLAGKIAKSCGIEDSERFFIEGLLRDIGHLVLYQTIPQRAQSAL
                     IEAGYLETSLAEVEQSNIGCDFAEVGAELIRSWGMPVQIEQAIRCQLSPNDAGEFIIH
                     ASIVHLAGVVADYEELEPSRRPATLPFNHHAVTATRFVSANLPALLTDARAKLQDALA
                     LIHPRTMAA"
     gene            5649..5912
                     /locus_tag="VN647_05595"
     CDS             5649..5912
                     /locus_tag="VN647_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96537.1"
                     /translation="MSRIGILSAPLSYNRQQPEWLGRPALLSLSWVIESRRKEYYDAL
                     PPPHATFKIWWTKKYSLLPAAAGAHITRDERSRVLELVANLRE"
     gene            complement(6017..6481)
                     /locus_tag="VN647_05600"
     CDS             complement(6017..6481)
                     /locus_tag="VN647_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96538.1"
                     /translation="MLVVSVLAVSVAACADTGQIQRELAPPDDPVIGLLSKGITQLNV
                     NINMVSKRMNDVRQASAGTDSALQELQALDLSGWELQQRQWIVQRNHLVLTRDTLHRV
                     QLSQEEKAQALAQWQQHRRQYVKAMEDLRQQRHQLESKHLEVEARLIERGLQ"
     gene            complement(6610..7269)
                     /locus_tag="VN647_05605"
     CDS             complement(6610..7269)
                     /locus_tag="VN647_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96539.1"
                     /translation="MPCILAAGRAADEGRRLRADAKFRMERYIATGPIIIGLCLCFSA
                     CARVDTILLTNDTFPPKESAEEVAVLRQTPTRPHRDLAELRISDNRLSFGSLQHKILN
                     RAAALGADAVVFAQPQTRTIRRESYQPLYGSYGPWGYNSPYYGGPWGYGMYGGLYGGW
                     GPWGGVPFGSVAGPYEYDETVRILTGTAIRYTDAFGPQSSIRSDEPGSQTLTLRRTGY
                     R"
     gene            complement(7650..>9358)
                     /locus_tag="VN647_05610"
     CDS             complement(7650..>9358)
                     /locus_tag="VN647_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWG96540.1"
                     /translation="GKSTLLKILAGLEDPDRGTRSMRRQLRIGYVPQEPSFADQHSVE
                     DALSQVLLDEGLDPHEQGARLAKAVSLGGFVRSDQSVSTLSGGWKKRLAIARSLILEP
                     DVLLMDEPTNHLDVEGILWLESLLKAEPHAFIVISHDRRFLESVTTRVWELNRRYVNG
                     VFQVNGRYSEFLEQRDAALQAQADYQASLANRVRREVEWLRRGPKARTTKAKARIDSA
                     GRLIDELNDIESRQGNAPAGIDFTASGRKSTQLLVAKGIEKSLGGKLIVSGVDMLLGP
                     RERIGLLGPNGGGKTTVLKLLAGTLEPDSGTITRADRLRVVTFEQHRESLDQQVTLRR
                     AFTPAGGDAVVYQERSVHVVSWAKRFLFRPEQLDLPISRLSGGEQARLLIARLMLQPA
                     DLLILDEPTNDLDIPTLDVLEDSLLEFTGALVLVTHDRWLLDRISTRLLALDGMGRAE
                     WFADYVQWEAAQVRNATEERRTQVSKESSAQAKPSKRKGLSYKEQKEWDQIETKILEA
                     EGCVVACQAATNHPSISSSATDLQERYAALHVAQTEVERLYARWAELDEKRAQVITST
                     QP"
BASE COUNT         2141 a         2658 c         2497 g         2062 t
ORIGIN      
        1 agcgattttc tctgggaaga ttttctgcgt cgccaataca gcaacctgct gccgctgttg
       61 caagattaca gggatgcgcc cctcatgacg ccgcgagcga cgcttcgcaa ggaacttgac
      121 cgcctggtga atcgagccaa aaccgacgag gcgaggaagg cggcgctgaa cagctttaaa
      181 gatcgcgagc tgttccgcat cgacatgaaa catatcgtcg agccgggcac cgccttgccg
      241 gatttctcgg ccgccctgac tcaactggct gaagtcatcg tcgatcgaag cttgaaggac
      301 tgtcaggcta agctgaacaa gctctatggc ctaccgcgat tgacgaataa aaagccttgt
      361 cctttcaccg tcctcggctt gggcaaattc ggcggcaagg aactgggcta tgcctccgat
      421 attgaagtga tgttcgtcta tggcgacgca ggccgaaccg gcggcaagca accgatcgag
      481 aacagcgaat actttgagcg gctcggcacg gaactcttac agtggatcga ggccaagcaa
      541 gagggcatct ttcatctgga cgtacgactc cggccacacg gggggaaagg ttcgctgacg
      601 aattcgtttg atgagatcac cggctactac agcaatcatg ggctcgccgc gccgttcgaa
      661 cggcagtcgt tgatcaaatt gcggcatgtg gccggggatg ccgccctcgg aaaacgcgtc
      721 gaagcccatc gtgatggtta tgtctacagc ggtaaacctt ggaatcttcg tatcgccctc
      781 gacttgcgtc ggcaacaagt gaagcaactc gtggagcggg gcacgatcaa cctcaaacat
      841 agccccggcg ggattgtcga tatcgaatac gcggtccagt acctccagat catgcacggc
      901 cctcggtttc caattctcca tactcccaac accatgcagg cgctcgctgc cttggttgat
      961 tgtggcattg tcacgaggca agacggcgag accgtccgca aggcctattt cttcatccga
     1021 atgctcatcg acggtctccg catggttcga ggcaatgcca aagatcgtgt cctcccacct
     1081 gtcgattccg acgaattcat cttcctcgcc cgccgcgtcg ggtatacgac cgatgattgg
     1141 caagcaggag caagacattt ggaaacggat gtacaagagc acatgggaaa agtgaaggag
     1201 ctttttacga ggacgtttgg gaaggtgtga ctgcagataa ggaatgaaga aacgtcgttg
     1261 cgagaagatt cagcccagca ggcataacct taatcgttta aggttttagg tatggcttgt
     1321 tacatcggcc tgtcaggcta ttcatacaaa gcttggcagg ggcctgatcg cttttatcca
     1381 tccggtatca aaccaaccga tttccttcgt tactatgcca cgaaatatca aaccgttgag
     1441 ctcgacggcg tgtggtatcg gctcccgatc gagaaaaccg tgcaggcatg gctcatagat
     1501 acccctcctc acttcatcta tgcaccaaaa gtccaccggg aaattacaca tgtccatcgc
     1561 ctcaagcctg aagggctccc catgttgaga gccatgctgc aacggctcga accgcttcat
     1621 aaggcaggta aactcggccc gatattgatt caattgcccc caaacttaca acgtgatgac
     1681 ggacggcttg agacgtttat tgagtctctg ccagcgaccc atcaatgggc cgtagagttt
     1741 cgccatgaga gttggcaagc tgataatgtc gaagggatct tgcgccggta tgcggtcgca
     1801 tgggccgcag tggagacgga tgagcatgag gcagagcacc gggacacgac tcgatttcgc
     1861 tatgttcgac ttcgtaaaag tgaatacggg caggcagagc ttcgacgatg ggctgaacga
     1921 tttcagaatg cctttcaaca gggacaagac tgctttgtct attgcaggca cgaggacgag
     1981 ggttctccct ggctttgggc agacgaactt caccggctct tgaattccta gacaattcca
     2041 aaacggcagc cttagggctg aatcgacttg ccggtgtact ctacatcggt acataaaatc
     2101 gacttcgctg cggctaccga atagacctgc gttgtttaat ctcagccgag aatcgcgcct
     2161 ccacacggct cagcattctt caatgacata atcggtacgg atctctcctc caaagttggg
     2221 ctgttttcag atacctccct aggtatcctt cgccggcaca gaatgcgccc caaatagcgt
     2281 ccaacttact caacgaggtg acggctcatg atctccgatg aacgcttcac gcgatttatc
     2341 cgctcaggat tcctgcttgc tccactcttt cttcttgcct gttccagtgg tggcggagaa
     2401 ggaagcgtcc cgtcaggttc cccattgaca cctgccggcc ctccgattac atggacgctg
     2461 aagaacccaa tgccgacacc gcgcacggaa tttggcgtgg cgacagtcaa caataagatc
     2521 tatgcgatcg gcggttactc gaactcggga tcagttctcc aaacagtaga agaatatgat
     2581 cccaccacag atacatggac tcgcaaagcg gacatgccga ctccgcgtcg acaattgatc
     2641 gtcgtctccg tcaataacaa gatctatgcg atcggcggag tcaacttttc ctcagatccg
     2701 aataacctga tctattctta ttccacagaa gaatacaatc ctgccaccaa cacatgggcc
     2761 tcgaaagcct ccatgccgac tggtggcgca gtgaacaatg tgcttgggaa tcgttttatc
     2821 ggtggggctg ttgcaaacgg aaagatttat atcgcagtcc acagcaatcc agggacgctc
     2881 tcaccgatca cacacacact cgagtacgat cccgctgcaa atatgtggac gagtaagaca
     2941 caacctccgt ttagttacac gcgatatacg gtggcatcct tgaataacaa ggtatatgcc
     3001 ctttccgaca tcggggaact ggcggagtat gatccactaa aagatagatg gatcatccga
     3061 cctccattat ccacaccccg gttccgcacc ggcctcgcct ccgttggagg gaaacttttt
     3121 tccattggcg gagggcagaa ctcctctcct gtcaacaccg tggaagagta tgatcctgag
     3181 acccagaact gggccttgag agcgtccatg gcttctcccc gcgtctcagt tgcagtcgga
     3241 gaggcgctcg gcaggcttta cgcgattggt gggtcctcaa ataccagcga gaccttcccc
     3301 tccccccttg ctgcggtaga agaaggcacg ccccctcctc ccgggtcagt ccttcagatt
     3361 cccacgggag acgtagtcac gaccggcaac ggtcaagtaa ccgtccggtg gaatcctgtt
     3421 gccggagcaa catcttataa cctctacatg gcctcggaca tcaaccttga tcggagcaac
     3481 tatgccggct tacccaatgg agcaaagttt gtcggtgtga catctcccca tacaatcacc
     3541 gggttgacca atggcaaaac ctattacctc ttcgtgactg cggccgatgt tggtcgagaa
     3601 agtgaagaat ctttcggctc ttctgctact ccaacagctc caccaggcct gctctggcgc
     3661 acgaaagcgt cgatgccgac acctcgcctt gaagccgggg ctgcagtcgt gaatggtaaa
     3721 gtctatgtcg tcggaggatt ttcaggaagc gcgctggcga caaccgaaga atacgatccg
     3781 gttacggaca catggacgac ccgggccgac atgcctaccg cccgccgctc tccggttgtg
     3841 gccatggcaa acaacaaaat ctacgcaatc ggagggatga gttacattga tgtcaaccaa
     3901 gtgacctact cctatgcaac tgaggagtat gatccatcga cgaatacgtg gacttccaaa
     3961 acttcaatgc cgaccggtaa ttccgtgaac tccgttctcg gaaatcgttt tatcgccggc
     4021 acggctgtga acggcaagat ctatatcacc gtgttcaaca acggcggcac cccaccgatt
     4081 tcctcgacgt ttgagtacga tccagcgacg aatacgtggg ataccaacaa gtcaccagtc
     4141 cccttcggca atgcccagta tacagtggca tcattgggcg ggaaattgta caccctcacc
     4201 tccggctcat ttgcagaata cacccccgca actaacacat ggatcattcg accatcccct
     4261 ccacaaactt tctcccaaat gagactcgtt gcagatgtca cgaagaacaa gctgtatgcg
     4321 attgggggct ataacgcctc ggatatccat gacaccgtac aagaatacga ccccacttcc
     4381 aatgtttgga caacccaagt ctccgtgccg gttccgagac attctacgac gaccgctgcg
     4441 ataggaggaa aagtatacgt gttcggaggc tcgcttgttc tcgatttcat caatgcggca
     4501 ggagtcccga catttattag accacttccc ttatcagatg tagaagaagg tcaataagga
     4561 aggcatcgct ttctaagact ttcagctcgt gatcacattc cggcccgtag gatcagaaga
     4621 tgctgtgcat tcaccaaagg cggtgaagag ctcacttcaa acggccgagc ctatctccgg
     4681 tcccagcaac cttctgtcat gttacgcggc catggtgcgg ggatggatca gcgccagggc
     4741 atcttgcagt ttggcccgag catccgtgag caatgccgga aggttggccg acacaaatcg
     4801 tgtggcagtg actgcatggt ggttgaacgg caatgtagca gggcgccgac tcggctcgag
     4861 ctcttcgtaa tcggcgacga caccggccag atgaacgatg gatgcatgga tgatgaattc
     4921 accggcatcg tttgggctca gttgacagcg aatggcctgt tcgatctgca ccggcatgcc
     4981 ccaggaccga atgagttcag ctcccacctc cgcgaagtcg cacccgatgt tggactgttc
     5041 cacctcggct agagaagttt cgaggtagcc cgcttcaatg agagcggact gggcccgctg
     5101 cgggatggtc tggtagagga cgagatggcc gatatcgcgc aatagccctt cgataaaaaa
     5161 acgctcgctg tcctcgatgc cgcaggactt cgcgatcttt ccagcgagca aggcacagag
     5221 cacgctttta cgccagaaca ttgtgacgtc catgaattga atcggcattc cggagaaggt
     5281 ccgtccgacg gtcgtcgcag taaccagatc gttgatggct tgcataccga cgatgttgac
     5341 ggcgcgggaa atggtgtcga tctgtttggg aaacccatag aggggactgt tgacgataca
     5401 aagaagtctg gccgagatgg ccggatccag cttgaggacg tcggcaaggt cgtccatggt
     5461 cgaatcaggg tcgtccacca cctcccgcac cctggaatag atctccggca aggtaaagac
     5521 cgtggtgcag gactgaacca gttcgctcgc caaaggcata gggaatcctt tcagttttaa
     5581 tcgatggagg ccggcttccg ccttctttat cggctatcaa caagtagaac tgtagaagaa
     5641 gcggtctcat gagccgaata ggtatattat cggcccccct gtcctataac cggcagcaac
     5701 ctgagtggct cggacggccc gccttgttga gcctatcgtg ggtgatcgaa tctcgaagga
     5761 aggaatatta cgacgccctg ccaccgccac acgcgacctt caagatttgg tggacaaaga
     5821 agtactcgct cctaccggcg gcggcaggag cacacattac tcgagatgag cgctcgcgtg
     5881 tcctggagtt ggtggcgaac ctccgcgagt agggccgggg ggtcgacctg agcgaagagc
     5941 gtggcgagga tgacgttagg cgaagagtgg ttctgaacca cgtatcgtga tgacgaacct
     6001 cgcgcaggtg acactgtcac tggagtccgc gttcgatcaa ccgggcctct acctccagat
     6061 gtttgctctc caactggtgt cgctgttggc gaaggtcttc cattgccttc acatactggc
     6121 ggcggtgctg ttgccattga gcgagcgctt gggctttctc ttcctgactc agctgaaccc
     6181 gatgaagagt atcgcgcgtc aagacgagat ggttgcgctg cacaatccat tgccgctgct
     6241 gaagctccca gccggacagg tccagcgcct gcagttcttg caaggccgaa tcggtcccag
     6301 ccgacgcctg ccgaacatcg ttcatgcgct tcgacaccat attgatgttg acgttcagtt
     6361 gcgtgatgcc tttgctcaag aggccgatga ccggatcatc gggaggggca agctcacgct
     6421 gtatttgacc ggtgtcggca caggctgcga cactgacggc aagcacactt acgacaagca
     6481 ctcttaatcc acgtctgttc atacatcctc actcacgaaa gagtcatgac atccgtcaga
     6541 cggcccgctg atggctcctc accatctcct ttttcctcgc atccttcttc agccatgaag
     6601 gaggtccatt tatctataac ctgttcgtcg caaggtgagg gtctgtgacc ctggttcgtc
     6661 ggatcggata ctgctctgcg ggccgaatgc atcggtatac cggatcgccg ttcccgtgag
     6721 gattcgcacg gtttcatcat actcgtatgg acccgcgaca ctgccgaacg gaaccccacc
     6781 ccaaggaccc catcctccat atagaccgcc atacatgcca tatccccagg gacctccgta
     6841 gtatgggcta ttgtagcccc acgggccata cgagccatag aggggttgat aggactcccg
     6901 gcgtatcgtc cgagtttgtg gttgtgcaaa caccacggca tcggcgccaa gggcggcggc
     6961 tcgattcaag atcttgtgtt gcaggctccc gaaactgagc cggttgtcgc tgatccgtaa
     7021 ctccgcgagg tcgcgatgag gcctggtcgg agtctgtctc aacacggcga cctcctctgc
     7081 agactccttt ggaggaaagg tatcgttggt gagcaagatg gtatccaccc ttgcgcatgc
     7141 ggagaagcac aggcagaggc cgatgatgat cggaccggtc gcgatgtatc gttccatgcg
     7201 aaactttgcg tcagctctga gccgcctccc ttcatcggcg gcacggccag ccgctaaaat
     7261 acagggcaca accttactcg cccatccgac cgttcacccc ggcctggaca acatggttgc
     7321 attcagtacc agcttcccgc atttgagcaa ataaatgcaa gaccagtggg aagagaggag
     7381 gcaagagagc gtgactcttc ttggccaagc catgttccaa caacagcaac aacacctgta
     7441 ggtctcgccg cccagtgcgc ttgagaattc aacctgatgc cggctgcttc accacagcga
     7501 acggtttgtg atttacagag gagaggcgct tctgcggctc gacacataag tcaatgcgtc
     7561 acctacctta taaagatgcc ttgagtctat gagaagccct caaattatgg tgacgcgagg
     7621 ccacatacgg tttattgcct gccctgccac tatggctgcg tactggtgat cacttgagca
     7681 cgtttctcgt cgagttcggc ccagcgggcg taaaggcgtt cgacttccgt ttgggcaaca
     7741 tgaagtgcag cgtaccgttc ttgtaaatca gtagcggatg aggagatgga tggatggtta
     7801 gttgcagctt gacaggcgac tacgcatcct tccgcttcta agatcttcgt ttcgatctgg
     7861 tcccattcct tttgttcttt gtacgacagg cctttccgtt tggatggctt cgcctgcgcg
     7921 gagctctcct ttgagacttg agttcttcgt tcttccgtcg catttcttac ctgcgcggct
     7981 tcccattgga catagtcggc aaaccactcg gcgcggccca tcccatcgag ggccagaagc
     8041 ctggtggaga ttcggtccaa gagccaccgg tcgtgtgtca ccaaaacaag cgcgccggta
     8101 aattccagca ggctgtcttc cagcacatca agggtcggaa tatccaaatc attggtcggc
     8161 tcgtcgagaa tcaagagatc tgccggttga agcatgaggc gtgcaatcag cagacgggcc
     8221 tgctctccac cggatagacg tgaaatcggg agatccagct gttccggcct gaagaggaag
     8281 cgcttggccc atgagaccac gtgaacggac cgttcctggt aaacgacggc atcgccaccg
     8341 gctggggtaa aggcgcgtcg caacgtgact tgctggtcca atgactcgcg gtgttgctcg
     8401 aaggtcacca cgcgcagccg atcggcacgc gtgatggtgc cactatcagg ctctagggta
     8461 ccggctaaga gcttgaggac ggttgttttg ccgccaccgt tggggccaag gaggccgatc
     8521 cgctcccgcg ggcctaacaa catgtccacg cctgatacaa tcagtttccc tcccagcgat
     8581 ttctcaatcc cttttgcgac cagtaactgc gtggactttc ggcctgaggc tgtgaagtcg
     8641 atcccggcgg gagcatttcc ttggcgcgat tcaatatcgt ttagttcgtc aatcagccga
     8701 ccggccgaat cgatacgggc cttagctttg gtcgtgcggg ctttcggccc gcgtctaagc
     8761 cactccactt cccgacgcac ccgattggct aacgacgctt gatagtcggc ctgcgcctgt
     8821 aatgccgcgt cgcgctgttc caaaaactcg ctgtaccggc cgtttacttg aaagacgcca
     8881 ttgacgtatc ggcgattcaa ttcccagacc cgcgtggtca ccgactctaa gaatcgccga
     8941 tcatgactga tgacgatgaa ggcatgaggt tcggctttca acagactttc aagccatagg
     9001 atgccttcga catccaaatg gttggtcggc tcatccataa gcagcacatc cggttccaat
     9061 atcaacgacc gcgcgatcgc cagccgtttc ttccatcccc ccgacaacgt cgagacggat
     9121 tgatcagaac gaacaaaccc gccgaggctg acggctttgg caagacgcgc tccctgctca
     9181 tgcgggtcta acccctcgtc cagaaggact tgggacagtg catcttcgac cgagtgttga
     9241 tcggcaaatg agggttcttg cggcacatag ccgatacgaa gctgccttct catcgatctg
     9301 gtgccgcgat ccggatcctc cagaccggcc aggatcttga gcagcgtcga ttttccag
//
LOCUS       DATNDD010000234         5787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_720870, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000234 DATNDD010000000
VERSION     DATNDD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720870"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VN647_05615"
     CDS             complement(<1..372)
                     /locus_tag="VN647_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96541.1"
                     /translation="MIKILLVEDNDVDARLTQDILAEWGMEEFDIIHVTRLSDAVTHL
                     ARARFDAVLLDLSLPDGYGLSTVRQMQAANPTVAIIVLSGLNDQTLALQAVQNGAQDY
                     LVKGEGQSELLARSIRYAIERK"
     gene            1199..2515
                     /locus_tag="VN647_05620"
     CDS             1199..2515
                     /locus_tag="VN647_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96542.1"
                     /translation="MRLFLASLSLAQKLRETIELCSLLRITLDPHEVRPMEELDSLGV
                     LWNLLTRDVAVVGGLQSPLLSWIGAAGIFTLCLWHSAVLIRGQVHIQRTLSRLQSSVA
                     PLVLARRQVSKDWLVIPALAKRRSNLAESPEARRDLDDLQALDRAVRSEQAFARDWLS
                     YRKTLAVEQSAWFLEPTVHSQRSASEFFSFESLCAAHLNVRFYRQLPAFMTGMGLMFT
                     FLAILIGLSKLHANGSEIDGIQGLINGLSGKFVTSIVGLACANAFTLLENSLWHRLDN
                     RHRECISLLDEMFPKKAADQSARISSLPNGSPATIVAPVRNDAAHQLVEAVQQRLGST
                     VAALNSAAQALTALNSKHSPMKLDDLCEEIGHEVQRALRPMMNPLLEAIHDLNRSIKQ
                     QCSSVQLSHSEIETMFQELRSNTAATTEPVRPANNDRDPGKSSRIT"
     gene            2540..3172
                     /locus_tag="VN647_05625"
     CDS             2540..3172
                     /locus_tag="VN647_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWG96543.1"
                     /translation="MKSIFAQDSRDNSAVVTSGIADLMTSLAVIFILLLVAYVTRVHD
                     GNTDPAGSRAAPADMTPSFDPRPSPLETKRPSVHTVTVPDAAINFEFGKSALLPAAEA
                     FLSEVMPHYASITCQSGGQEVEAFVIEGYTDDLGDDIRNLRLSQDRSFAVLAKSLEVI
                     REKLPWAYECFLQKATANGRGKQNLLRNDAGHLDRDKSRRVIFKIHMRPA"
     gene            complement(3224..4045)
                     /gene="rsmA"
                     /locus_tag="VN647_05630"
     CDS             complement(3224..4045)
                     /gene="rsmA"
                     /locus_tag="VN647_05630"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWG96544.1"
                     /translation="MDSSLPPAAIKRLGQNFLIDPNIVRKIVALAELTPTDTVLEIGP
                     GRGILTEALCRAVGHVTAIEVDPRLHIYLTERRPRLSNLALVLGDAMTYPIEHLPIDT
                     IVVANLPYYASTPLLFRLLDQRNRFPRMVLMLQNEVADRLMAKPGGSDYGVLSVMAQY
                     AAEITKAFRVSAQCFRPRPEVDSAVVLLKTRHQRALNREEEPKFSALVKAAFAHRRKT
                     LVNSLKDQGYEQKSVAAASESLNLSPSTRAEVLSIEQFIELTRRIYGTGFSNLRR"
     gene            complement(4049..4369)
                     /locus_tag="VN647_05635"
     CDS             complement(4049..4369)
                     /locus_tag="VN647_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96545.1"
                     /translation="MSPDMSKGLTVVRQQIDELESLKKQTLQDLNTIAGTERIAQWKG
                     HTVALITDAVGHLEGQAFARIQPGPSFTNDLVEEFADLVECYRSPLIILAKRLAAQAP
                     PPER"
     gene            complement(4359..5372)
                     /gene="pdxA"
                     /locus_tag="VN647_05640"
     CDS             complement(4359..5372)
                     /gene="pdxA"
                     /locus_tag="VN647_05640"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWG96546.1"
                     /translation="MGDPSGIGPEVIAKALSGARLHNVCRSIVIGSLPVMERTITALK
                     LKLKVFRVHGHETTAPRRGTVAVLDPLDTPLRAFKPGTAAAETGAASVVFIKKAVELA
                     QIGCIEGMVTAPINKEAIYMAGCRYPGHTELLADLTHANESGMMIVGGPLRIMFVTTH
                     VAIRDLSLLLTQAKIEKAIRLAQSALTTLFGIKRPRIGVAALNPHAGENGLFGDEEAR
                     VILPAARATQREGILASDPLPADTLFGKAAKGSFDGVVALYHDQGLIPLKLVAFGTCV
                     NLTVGLPIIRTSVDHGTAFDIVGKGVADPGSLIEAVKLAAKIARNRTAATPAKKGRAG
                     HVA"
BASE COUNT         1246 a         1622 c         1637 g         1282 t
ORIGIN      
        1 ctttcgttct atggcatagc ggattgagcg ggccaacagc tccgactgcc cctccccctt
       61 gacgaggtaa tcctgtgcgc cattctggac agcttgtaat gcgagcgtct ggtcgttgag
      121 cccgctcagc acgatgatgg caaccgtcgg gttcgcggct tgcatttgac gcacggttga
      181 gagtccataa ccgtccggga gtgataggtc gagcaggacg gcgtcaaaac gtgcgcgggc
      241 caggtgagtc accgcgtcgc ttaggcgggt cacatggatg atatcgaatt cttccatgcc
      301 ccattccgcg agaatgtctt gcgtcaggcg tgcatcgacg tcgttatctt cgaccaatag
      361 aatcttgatc atcgcggtct caatgccgtc cgttttcctt ccctagcgtg cattattcac
      421 caagtatagc gtaggacact gattcaacaa gcgaattacc gaagtacgga cctcccaaga
      481 tattccgtct cgtgagtgtg aatgccccga taaatcgttt tggctttcag aacctggatg
      541 aggcttcaga aatccgcgct caccagccgg tgagacgctt gctgatgaag gcagactgga
      601 ccactgtttt tcccaacatc tccatcgggg accgtcgggc gccggtcctt cgtcgcgccc
      661 gaggtcatta aaaggaagag ggtgcggaca aaggagcgac tcagtcagga gcctgataaa
      721 cggcctgggg actgcctggg tacgtaccga ttggacctcg cggtgagccg atcgccgcct
      781 cacaagcgta ccccggttga aagggccata atagttcacg accgacggtg ttcccacgta
      841 tatgaacgta ccgtaacgtg acgcccatgc gagcggcttc gtccttaacc tttcccgtac
      901 attcatcggg tgtataccct cgacgtgaaa tcaggagcgg ctctccggat gcgctgaaaa
      961 cggagaattc gctcgcgcat cccatcatcg aacataccac acacatcatg ccggcaagtc
     1021 gactcacacg tgttcttgaa agcatcggag ccgcctcatt gtgaatgcct tctgttcatc
     1081 ccaaaacaaa cagcacatca aaccataaca cgaaagccga gcttcaatcg accgaaattg
     1141 cctccttctc ggcaaaaagt tccatcctgt catggcccta gcccgacagg gtatcggtat
     1201 gcggctattt ctcgcatccc tctctctggc acaaaagttg agggagacga tcgaattgtg
     1261 ctcattgctg agaataacgc ttgaccccca tgaggtccgt ccgatggagg agttggattc
     1321 actcggcgtc ctttggaatt tgctcactcg cgacgtggcg gtcgtcggcg ggcttcaaag
     1381 tcctctgctt agctggatcg gtgctgcggg gatcttcacc ctttgtcttt ggcatagtgc
     1441 ggtgctcatc cgaggtcagg tacacattca acggactctt tcgcggttgc agtcgtccgt
     1501 cgcgcctctg gttctggcac ggcgacaggt ttccaaggac tggctggtca tcccggcgtt
     1561 ggcaaaaaga cggtcgaatc ttgctgagtc gccggaagcg cgacgcgatc ttgacgatct
     1621 gcaagccctc gatcgtgcgg tacggtcgga gcaggctttt gccagagact ggctttcata
     1681 tcgcaagacg ctcgcggtag agcaatcggc ctggtttctt gagccgacag ttcacagcca
     1741 gcgctccgca agtgagtttt tttcgttcga atctctctgc gccgcccacc tcaatgtccg
     1801 gttttaccgg cagcttcccg ccttcatgac gggtatgggt ttgatgttca ccttccttgc
     1861 tattttgatc gggctcagca agctccatgc gaatggatcg gaaatcgatg gaattcaagg
     1921 gcttatcaac gggctctcag gaaagttcgt cacatccatc gtcggcttgg cctgcgccaa
     1981 tgcgttcact ctcctcgaaa attctctgtg gcatcggctg gacaatcgac accgggaatg
     2041 catttctctc ctggacgaaa tgtttcccaa aaaagctgcc gatcaatcgg cccgaatttc
     2101 atcattgccg aacgggtctc cagcgaccat agtcgctccg gtccgaaacg atgctgcgca
     2161 tcagcttgta gaggccgtgc agcaacgcct cggatcaacc gtcgcagcct tgaactccgc
     2221 cgcacaagcg ctcacggctc tgaattccaa acattcaccg atgaagcttg atgatctgtg
     2281 cgaggagatc ggccacgagg tccagcgggc gttaagaccc atgatgaatc ccttgctgga
     2341 agccattcac gatctcaacc gttccatcaa gcagcaatgc tcatcggtgc agttgtctca
     2401 ctcagagatc gaaacgatgt ttcaggaact caggagcaat acggctgcaa cgacagaacc
     2461 ggtacgtccg gcgaataacg atcgcgatcc gggaaaatca tctcgtatca cgtgacaccg
     2521 aacggaaggc acggagggga tgaagtccat cttcgcacag gactctcgtg acaactcggc
     2581 tgtggtgacg agcggaattg cggatcttat gacctcgctc gccgtcatct tcattctctt
     2641 gctcgtggcc tatgtgactc gagtccatga tggaaatacc gatccagccg ggagccgtgc
     2701 cgcaccggca gatatgacgc cgagttttga cccaaggcct tcgccgctgg aaaccaaaag
     2761 gccgagcgtt cacaccgtca cggtgccgga tgcggcgatc aactttgagt tcgggaagag
     2821 cgcgttgctc cctgctgccg aagccttctt gtccgaggtc atgcctcact acgcctcaat
     2881 cacttgtcaa tctggtggtc aagaagtgga ggcctttgtc attgaagggt acaccgacga
     2941 tctgggtgac gatatccgca atctgagact cagtcaggac cgctcttttg cggtattggc
     3001 gaaaagcttg gaagtgattc gcgagaagct tccttgggcg tatgaatgtt tcttacagaa
     3061 agccacagcg aacgggcgtg gaaaacagaa ccttctacgc aatgatgccg gacacctgga
     3121 ccgcgataag agccgtcgcg tcattttcaa aatccacatg cgacccgcgt agcgtgccga
     3181 gcacgcccga cgccggttgt ccaagcatcc cgagcggcag ttgttatcgg cggaggttcg
     3241 agaagcccgt cccatatatg cggcgagtca attcgatgaa ctgctcgatg gaaaggactt
     3301 ccgcacgggt ggagggagag agattgaggg actccgatgc tgctgcgacc gacttctgct
     3361 cgtacccctg atctttcagc gaattgacca gcgttttacg gcggtgcgcg aaggcggcct
     3421 tcacgagtgc ggaaaatttt ggctcttcct cccggttcag tgctctctga tgcctggtct
     3481 ttaggagaac cacggcagag tcgacttccg gtcttggtcg aaaacactgc gccgagactc
     3541 ggaacgcttt cgtgatctcc gccgcatact gtgccatgac ggaaagcact ccgtagtcgg
     3601 agccgcccgg ttttgccatc aagcgatctg cgacctcatt ttgcagcatc agcaccatgc
     3661 ggggaaaacg gtttcgctga tcgagaagcc ggaagaggag aggtgtggac gcataatagg
     3721 gaagattcgc gacgacgatg gtatcgatcg gaaggtgttc gatcggatag gtcatcgcat
     3781 cgccgaggac gagcgcgaga ttcgaaagcc ggggccgtcg ttcagtcagg taaatgtgga
     3841 gtcgtggatc gacctcgatt gccgtcacgt ggccgacggc acgacaaagc gcttctgtga
     3901 ggatgccgcg tccagggccg atttccaaga cggtatcggt cggggtcagc tcagccagcg
     3961 cgacgatttt acggacaatg ttgggatcga tgagaaaatt ctgtccgagc cgtttgatcg
     4021 ctgcaggggg gagagaggag tccacccgtc acctctcagg tgggggagcc tgggccgcca
     4081 atcgcttggc tagtattatc aacggcgagc gataacattc gacgagatcc gcaaattctt
     4141 cgaccaggtc gttggtgaac gacggccccg gttggatacg agcgaatgcc tgtccttcca
     4201 gatggccgac ggcatcggtg ataagcgcca cggtgtgccc cttccattgt gctattcgtt
     4261 cggtcccggc gatcgtgttc agatcttgaa gggtctgttt cttgagagat tccaattcgt
     4321 cgatctgctg ccggaccacg gtaagcccct tcgacatatc aggcgacatg tccggcccgc
     4381 cccttcttgg ctggagttgc cgccgttcta ttccgagcga tcttggccgc cagcttgacg
     4441 gcctcgatca gacttccggg atcggcgacg ccctttccga cgatatcaaa agccgttcca
     4501 tggtctactg aagtgcggat gatgggcaag cccacggtga gattgacgca cgtgccgaaa
     4561 gcgacaagct tcaggggaat gaggccctga tcgtgataca gtgcgacgac accgtcaaat
     4621 gaccctttcg cggcttttcc gaatagggta tccgccggta gaggatcact tgccaggata
     4681 ccttcccgtt gcgtcgcacg ggccgccgga agaatcacgc gggcttcctc atcgccgaac
     4741 aatccattct caccggcatg gggattgaga gcagccactc cgatcctggg gcgtttgatg
     4801 ccgaacagcg tcgtcaacgc cgattgagcc aagcggatcg ctttttcaat ctttgcttga
     4861 gtcagcagca acgagaggtc tctgatcgcg acgtgtgtcg tgacgaacat gatacgtaac
     4921 gggccgccca cgatcatcat gcctgattcg ttggcgtgag tgagatcggc caacaactcg
     4981 gtatggcccg ggtatcggca gccggccata tagatggctt ctttgttgat gggtgccgtc
     5041 accattccct cgatacagcc gatctgagcc aattcaacag cctttttgat aaagaccact
     5101 gaagcggcac cggtctctgc tgctgccgta ccgggcttga acgctcgcag cggtgtatcc
     5161 agtggatcca gtacggccac cgttcctctg cgcggcgccg tcgtttcatg accgtgaact
     5221 ctgaatactt tcagtttgag cttcagtgcc gtgattgttc gctccatgac gggaagcgat
     5281 ccgatgacga tcgatcggca aacgttgtgc aagcgagctc cggacagagc cttggcgatc
     5341 acttccgggc cgatgccgga cgggtctccc atcgtgattc ccagcaaggg gagtcggaaa
     5401 gagggggaac gtttgcgagc tgaccgcgac ttagtagttt gcggagaaat tcgttgatgc
     5461 ataacaatat ggctggaatt tcccataagg tgctaactcc gaacaacttc aggatgctca
     5521 aaagggctgt ccagcaaggc cgcagcgagc gaagaggtga ggcgtacgct tcggtacgtt
     5581 gaacctctga gcgatgcgag aacgccgccg ccggactttt tcagcatcct gtcagttgac
     5641 atagagatgc acggtatagc cgatatagaa cagagctccg gcggcgagta gccacggccg
     5701 ccactgaccc ccgatcagaa tctgcaggag gcagagtccc accgcctgca cggccaagat
     5761 catggtggtc aatgccgatg gcgcaat
//
LOCUS       DATNDD010000235        10072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_263478, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000235 DATNDD010000000
VERSION     DATNDD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263478"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..582
                     /locus_tag="VN647_05645"
     CDS             280..582
                     /locus_tag="VN647_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWG96547.1"
                     /translation="MTDERSSSQANQVEGQNARYLAPMGDLTIFEAEEFKESLVKLFV
                     NDGLVSLDLSAVARVDTAAIQLMLAARKQGRMFVTGISDDLQTKLNQLGFTDSLSE"
     gene            670..1212
                     /locus_tag="VN647_05650"
     CDS             670..1212
                     /locus_tag="VN647_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96548.1"
                     /translation="MNSSGNHDKRLERPLAGDETTLRALLEHQRAWETFAAESIHMIP
                     VLKAQMTAVTQETERAAMELLLHLRMLTSSEGEASSKDDSVNLSKVVMAMQFQDITRQ
                     KLEHVGLALDRLKAHLQALLKGPGDEEAKKEIAALELVEQHYTMEEERRLHTAALGPD
                     YGEPVPMDIASKEPDSVTLF"
     gene            1268..1639
                     /locus_tag="VN647_05655"
     CDS             1268..1639
                     /locus_tag="VN647_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96549.1"
                     /translation="MGKIALIVDDSPTMRQMVAFTLTNAGFTVVEAQDGKDAVDKVAG
                     GGKMDIVVTDLNMPEMDGIALIKELRKLSAFRFTPILMLTTESAAEKKQAGKEAGATG
                     WIVKPFNPEVLLKTIAKVLPA"
     gene            1733..3040
                     /locus_tag="VN647_05660"
     CDS             1733..3040
                     /locus_tag="VN647_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWG96550.1"
                     /translation="MSNDFSQFQEAFFEEAAEHLAVVEEGLLELEQHPEDVDLLNKIF
                     RSAHSIKGTAGMFGFNTVAQFTHKMETLLDLLRNGQKAVSPSIADLLLKATDCLKTLI
                     QSTKFGAAVDDEAVQRLTGELASASTSDSQISAVNVNGKVAQPVSAENGDGGQTYLIR
                     WTPPEWLFQRGLDPLQTFKELKSIGVLTGITLDMSRLPGLDCIDPETCYLSWTMQLTT
                     GKDLKVIESTFEFVREDSMLSIEPMKGTSKEEYAQNKADVSSEKGIPKQLGQILVEGG
                     LVSPGELDRALGQQKRVGEILVEQKVVTQQQVEQALQKQRQQESGAQTKKTDATSIRV
                     DTAKIDKLINLVGELVITQSMLSDLGSRFEMRRLPVLLERMVELERNTREIQERVMSI
                     RMLPIGTAFARFPRLVRDLSAKTGKKIQLILSGEETELDKTVI"
     gene            3065..3898
                     /locus_tag="VN647_05665"
     CDS             3065..3898
                     /locus_tag="VN647_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWG96551.1"
                     /translation="MVRNSADHGLEPPEERLDNNKQEVGTIRLNAFHEGGNICITVED
                     DGRGLDRDKIVAKANKQGLISENDKPTDDQIWPLIFKPGFSTAEKITDVSGRGVGMDV
                     VKRNIESLGGTVSIKTTKGKGTTFILKLPLTLAIIDGMTVRVGKETYIVPLLSILESI
                     QPKAEVIKTVVGKGELINVRSTYIPIVRLYEVFASEPQYTDPKEAILLILEAEGERVA
                     VMVDEILGQQQVVIKSMEQNFHKVEGIAGATILGDGRVGFIVDVRGVLEIARHGSPVA
                     A"
     gene            4121..4651
                     /locus_tag="VN647_05670"
     CDS             4121..4651
                     /locus_tag="VN647_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWG96552.1"
                     /translation="MSGAATDKEFKDLRRHAASHMDGGQFLTFSLGEELYGVDILRVQ
                     EIKGYTAVTKIPNTPPHIKGVLNLRGTIVPIVELRTKFGMPTIDYTTFTVIIVVVVQD
                     KVMGLVVDAVSDVLDIEKKDIQSAPDFGNKIDVTFLNGIGKSGDKLISLLDIDRLLLD
                     SAAKDSSKDLPVAAAA"
     gene            4982..7492
                     /locus_tag="VN647_05675"
     CDS             4982..7492
                     /locus_tag="VN647_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG96553.1"
                     /translation="MTILHVSALAAFCLMLAALCRWISRRPTEAAPLEERFHRLFEYA
                     AVAMSIVDSDKRFVHVNRAYSELLGYDTTELLGRPFTMVAHPEDIPEHLVLWDEVFRR
                     TECSSAQDKRYIRKDGRVIWVRVTANLFKPSDGTPPLVMAVVENITERKQAEQVLRQS
                     EARLIEAIPQQVWTARPDGTLDYVNRRVLEYFDVPAERLLDWGWQSVLHPDDLPACMT
                     AWTVSLQTGEPYEIEFRLRQAGAGTYRWHLTRALPMRDETGRIVKWIGTNTDITGRRQ
                     VEEALRESEERFRVLADTAPVLIWMSGRDNRISYFNTRWLEYTGRTMEQELGDGWVEG
                     VHSDDVDRCWNIYSEAFDRREPFDLEYRLRKADGQYGWLHESGAPRFLPGGTFAGYHG
                     ACIDITARKLAEAELRRVHDELERRVSERTAQLATANRSLQDEIAERKHVEEAVRDSE
                     LRYKLLTEATFDGIAIHNQGILIEVNAGLGRIFGYGPGELIGRSLWDLVADESRDLVL
                     ANMQNGVNGPYEAMGRRKDGTTFPGEVVVRPCRYRGKEVRLVAGRDITERKHLEAERS
                     RHTEELEHQIAERTAEIAKLESQRAQTEKLAAMGRLAAGVAHEINNPIAGIKNAFTLV
                     KQAVDPAHPHAEFVGMIDREITRVASIVQNMYQLYRPESGKGEAVELRSMMNDIEALF
                     SKRLQQRRLELAIDVDPCIDRLRVPRRDLLQVLLNLLNNAIDCSSDGGTVTLAICVEP
                     DAIRIAVSDQGPGISPDNLPHIFDPFFTTKTERDQRGMGLGLSISQSLVMAMGGKIEV
                     RTQPNGGSTFSVLLPRHTAGACAQDQSNIIKEVVTHAD"
     gene            7482..8240
                     /locus_tag="VN647_05680"
     CDS             7482..8240
                     /locus_tag="VN647_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96554.1"
                     /translation="MLTETPRILLADDEETFRRSTAKLLEQEGYRCDCAQDSHEAGML
                     LTRQYDALITDIRMPGNMQFEFLRDVRTRFPSLPIVLVTGYPSVQTAVEALRLSFTDY
                     LLKPVDWPDLLRAVGEAVKRTSLLRMTTAVREEASRLVASLEQLQDDLPRLGSGANER
                     ELAWSLDAFLSQSFAQMAVLSSRIRSVLTSQLQGGAEGPMDVCRMMQCPRLDAYREAL
                     ESTVEVLANTKHASRSQDLGLLRNKIEQLLREDA"
     gene            8751..>10072
                     /locus_tag="VN647_05685"
     CDS             8751..>10072
                     /locus_tag="VN647_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HWG96555.1"
                     /translation="MNTLRNLKTRPKLMISFGLVGLLLLTVGILSINGIDKLNERMNV
                     IYKVNLTSLKLLGDLHGQTERMSALVAWHILAYDSATVAKWTQEIGKLDEDVDRFVTE
                     YQPLIIAQSEREIYEHFKTSWAEYKDVRAKVMRLSANFSKDGAAELQRTELAEKLTAI
                     LGAIEGLTRENEMQAKETFDVSLDMASTLHTTNIVIVCVGLSLGLLLGWLISRSVSGG
                     LNDILNAAQALGAGNLAARSGITTKDDIGGLAQAFNRMGEQIESNVKESLESKYKMVA
                     LDRAEGVIEFNTDGTIITANQNFLNFLGYTLDEVKGHHQRLFAEPAYAASADYQAFWQ
                     RLNRGEYDAGVYRRLGKGGKEIWIQASYNPILDGAGKVSKIVAFATDITGQKQAQNEV
                     EKLIKAAAMGNLSDRIDTERFQGAAKDLVSSFNQLLDAVATPLHEAQGV"
BASE COUNT         2348 a         2583 c         3007 g         2134 t
ORIGIN      
        1 ggacgttgca aaaccctcca acggcgttct cgcgggacaa tgccgcctca cccaagacac
       61 tgggcgctca cgggacaccg cattcggcat ggcgtggact tcgccggcgc gcacaaatga
      121 ggacaccgag cgcacaccga ctcgcgcccg tgcgcaaact tgacgctcct tcttcgtcgc
      181 gtcgcgtacc tctttatcat cgcgccgtgc gcctcaagtc tcgatcaaaa cgagccgaca
      241 atatgatcca gtgatttcaa cgaatggagc ttacgaccga tgacagacga gcgatcgtcg
      301 tctcaggcaa accaggttga aggacagaac gccagatatt tggcgccgat gggcgacctc
      361 actatttttg aggcggagga atttaaggaa tctttagtga aattgtttgt gaacgatgga
      421 ttggtctcgc tggatctcag cgcggttgcc cgagtggaca cggcggcgat tcagctgatg
      481 ctggccgcgc gcaagcaagg gcgcatgttc gtaacgggta tttccgatga ccttcagacg
      541 aaattgaatc aactgggatt taccgactcg ctgagcgagt gatgcgcata tcgaggaggc
      601 caaggcggtg ccggacggca agtgcgacgg gtcgccgaga ggtgcgaggg gagccgcgtc
      661 agcggggcta tgaactcttc gggaaaccac gataaaaggc tcgagcgccc tcttgccggc
      721 gacgaaacga cgctcagagc gcttcttgaa catcaacggg catgggagac ctttgcggct
      781 gaatccatac atatgatccc ggtgttgaag gctcagatga cggccgtgac acaggagacg
      841 gaaagagcgg ccatggaatt attgctgcat ctccgaatgc tgacgtcctc cgaaggagag
      901 gcgtcgtcaa aagacgattc ggtcaatctg tccaaggtcg tgatggctat gcagtttcaa
      961 gacatcacgc gtcaaaagtt ggagcatgtc ggtttggcgt tggatcggtt aaaggcgcac
     1021 ctgcaggccc tattgaaagg gcctggagac gaagaagcca aaaaagagat tgccgccctg
     1081 gaactggttg agcagcacta cactatggag gaggaacgtc gcttgcacac ggcggcgtta
     1141 gggccggatt acggagagcc ggtacccatg gacatagcga gcaaagagcc cgattccgtc
     1201 acgctctttt agcgggaacg tccgatgctg tttcaatacg attctgtcac cgatgccgga
     1261 ggtcgccatg ggcaagattg cactgatcgt cgatgattca cccaccatgc ggcagatggt
     1321 ggcgtttacg ctgacaaatg ccggttttac cgtggttgag gcgcaagacg gaaaggacgc
     1381 ggtcgacaaa gtcgcaggcg gcgggaaaat ggatattgtc gtgaccgatc tgaatatgcc
     1441 ggagatggac ggtatcgcgc tcatcaaaga gttgcggaaa ttaagcgcgt tcagattcac
     1501 cccgatcctc atgctcacga ccgagtcggc ggcggaaaag aaacaggcag gaaaagaagc
     1561 cggcgcaacg ggctggatcg tgaagccttt taatcctgaa gtcctattga aaacgatcgc
     1621 gaaggtgctt ccggcatgac gaagggcata tggtatatcg cgtcacgcgt atggccgaag
     1681 tctcttcgtt tccgctgtcg gctgtcagcc atttgctctt ggagctaaga caatgagcaa
     1741 cgatttctcg caatttcaag aagccttctt tgaggaggcc gctgaacatc tggcggtggt
     1801 ggaggaaggc ttgttggaac tggaacaaca tccggaagac gtggatcttc tcaataagat
     1861 ttttcgctcg gcccattcaa tcaagggcac agccggcatg ttcggcttca atacggttgc
     1921 gcaatttacc cacaagatgg agacgttgct cgatttactg cggaatgggc agaaggcggt
     1981 ttcaccatcg atcgcggatc tccttctaaa agcaaccgat tgtttgaaga cattgatcca
     2041 atcaacgaag ttcggcgcgg cggtcgacga cgaagcagtc cagcgactca ccggagagtt
     2101 ggcctcggcg agcacatcgg actcgcagat atcggcggtg aatgtgaatg gaaaagttgc
     2161 gcaacctgtt tcagccgaga acggcgatgg aggtcagact tacttgatca ggtggacccc
     2221 gccggaatgg ctgtttcaac gagggctcga ccctcttcaa acgttcaagg aattgaaaag
     2281 catcggtgtt ctgactggaa tcacgctcga tatgtctcga ctacctggtc ttgattgcat
     2341 cgatccggaa acatgctatc tgtcatggac catgcaactg acgacgggaa aggatttgaa
     2401 ggtgatcgag tccaccttcg aatttgttcg cgaagatagc atgctatcga ttgagccgat
     2461 gaagggcacg agtaaggaag aatacgcgca aaacaaagcg gatgtctcaa gcgaaaaggg
     2521 gattcccaag cagctcgggc aaattctcgt ggaaggcggc ctggtctctc ccggcgagct
     2581 tgatcgcgct ctgggacaac aaaagcgggt aggcgaaatt cttgtcgagc aaaaggtggt
     2641 cacccagcag caggtggagc aggcgctgca gaaacagcgt cagcaggaat ccggcgcgca
     2701 gaccaaaaaa accgatgcca cctccattcg cgtggataca gccaagatcg acaagcttat
     2761 taacttggtg ggtgagctgg tgatcaccca gtcgatgttg agcgatttag ggtctcgatt
     2821 cgagatgagg cggttgccgg tgttattgga acgcatggtg gaactggaac ggaacacgcg
     2881 tgaaattcaa gaacgtgtca tgagcattcg catgttgccc atcggcaccg catttgccag
     2941 gtttcctcgg ctcgtgcgcg acctttctgc caaaacaggc aagaaaatcc aactcatcct
     3001 gtccggagaa gagacggaat tggacaagac ggtcatctag tccatcggcg atccgctcac
     3061 acacttggta aggaactcgg ccgatcacgg attggagcct cctgaggagc gattggacaa
     3121 taataaacaa gaggtcggga cgatccggct caatgcgttc catgagggcg ggaacatctg
     3181 catcaccgtg gaagacgatg ggcgtggatt ggaccgcgac aaaattgtgg cgaaggcgaa
     3241 taagcaaggc ttgatcagcg agaacgataa accgaccgat gatcaaatct ggccgttgat
     3301 cttcaagccg ggattttcca ccgcggagaa aataacggac gtgtcgggtc gtggtgtggg
     3361 catggatgtg gtgaagcgga acatcgaaag tctggggggg acggtcagta tcaaaaccac
     3421 caagggtaag ggcaccacgt tcatactcaa gctgccgctc acattggcga tcattgacgg
     3481 catgacggtg cgggtcggca aggaaaccta catcgtgccg ctgctgtcca tactcgagtc
     3541 gatccaaccc aaagccgaag tgatcaaaac cgtggtcggc aagggcgaac tcattaatgt
     3601 tcgaagcacg tacattccga tagtgcgctt gtacgaagtc tttgcgagcg agccccaata
     3661 tacagacccc aaggaggcca ttcttctcat cctggaggcg gaaggggaac gggtggcggt
     3721 gatggtggat gaaattcttg gacaacagca ggttgtcatc aaaagcatgg agcagaattt
     3781 tcacaaggtc gaaggcatcg ccggcgccac gatccttgga gacggcaggg tcgggttcat
     3841 tgtcgatgta cgcggcgtgc ttgaaatcgc tcgccacggc agtccggttg cggcctaatc
     3901 tttttgatac cgtcccggcg tttcatgcga aatctgtata cgatttcaat cggcggccct
     3961 tcaatgagct gtccactcca agtatgagat aaagcttttt taaaggaaga ccgatatatc
     4021 cttgaccgtc agtctattgt agaagtctgg acagacgccg acctgtcgga tcaagcagcg
     4081 gtgccgacga gacgggtgac gatcgggaaa ggagctttcc atgtcaggag cggctacgga
     4141 taaggagttc aaagatcttc gccggcatgc ggcatcgcac atggacggcg gtcagttttt
     4201 gacattcagt cttggagaag agctgtatgg tgtcgatatc ctccgtgtgc aggagatcaa
     4261 gggatatact gccgtgacca agatccccaa tacaccgcct catatcaaag gagtgttgaa
     4321 tttgcgcgga accatcgtgc ccatcgtcga actacggacc aagttcggta tgccgaccat
     4381 cgactacacg acgtttaccg tcattattgt ggtggtggtg caggacaaag tgatggggtt
     4441 ggtggttgat gccgtatcgg acgtgcttga cattgaaaaa aaggatattc agtcggcgcc
     4501 ggattttggg aataagatcg acgtgacgtt cttaaatggg attggaaagt ccggcgacaa
     4561 gctgatatcg ctgttggaca tcgaccggtt gttacttgat tcagccgcga aagattcctc
     4621 aaaggattta ccggtggcgg ctgcagcgta aggagtcgga ccgagcgccg catccggcca
     4681 cgaagccgtc ggccatgcca tctccctctc cctctcttct ttttcgtctc tccaagatta
     4741 tccattagtt gatttaagca gggtcttgcg tagggccata tggatttgac ggatgatgta
     4801 tggcgcgtta tcttgccgcc ccgtgaccca tcacggttgt cggacgacta ctgaagccaa
     4861 cgtcggtccc catcaagggg ctagccttcc atcttcaagc attctcggtc cttcgaccat
     4921 aggccttgtg gcgtttcttg gcttcaacgg cttctacctg gctatctcag ggcccgcttc
     4981 catgacgatt ctccacgtat cggcccttgc agccttttgt ctcatgttgg cggccctgtg
     5041 ccggtggatc agcaggaggc caaccgaagc tgccccactt gaagagcgct tccaccgtct
     5101 cttcgaatat gccgctgtcg ccatgtcgat tgtcgattcc gacaaacggt tcgttcatgt
     5161 gaatcgtgcc tactctgagc tgctcggata cgacacgacc gagcttctcg gcagaccctt
     5221 caccatggtg gcgcatccgg aagatatacc ggaacatctt gtattgtggg atgaggtgtt
     5281 tcgtcgcacc gagtgttcga gtgctcaaga caagcggtac atccgtaaag acggccgtgt
     5341 catctgggtc cgagtgacgg ccaacctgtt taaaccatcg gatggtacgc cgcctctcgt
     5401 catggccgtc gtcgaaaaca tcaccgaacg caagcaggca gaacaggttc tgcggcaaag
     5461 cgaagcacga ttgatcgaag ccattcctca gcaggtgtgg acggcccgtc ctgacggcac
     5521 attagattac gtgaaccggc gggtgctgga gtatttcgat gttccggcag agcgactgct
     5581 cgattgggga tggcagtctg tgctccatcc tgacgacctg cctgcctgta tgacggcgtg
     5641 gaccgtttct ttgcaaaccg gagaacccta tgagattgag tttcgcctca ggcaggctgg
     5701 cgccggcacc tatcgctggc acctgacccg cgccctgccg atgagagacg aaacaggccg
     5761 gatcgtcaag tggatcggga cgaacaccga catcaccggg cgccgacagg tggaagaggc
     5821 cctgcgggag agcgaggaac ggtttcgggt cctcgccgac acggcgccgg ttctgatctg
     5881 gatgtcgggt cgggacaatc gcatcagcta tttcaacacc cgctggttgg aatacaccgg
     5941 ccgaactatg gaacaagagc tgggagacgg ttgggtcgag ggggtgcatt ccgacgacgt
     6001 tgatcgctgt tggaacatct attcggaagc gttcgatcga cgtgagccct ttgatttgga
     6061 atatcgttta cgaaaggccg atgggcagta cggctggttg catgagagcg gtgcgcctcg
     6121 tttcctcccg ggaggaacgt ttgccggcta ccatggcgcc tgcattgaca tcactgcgcg
     6181 aaagctggcg gaagcggagc tacgacgagt gcatgatgaa ttagagcgtc gagtcagtga
     6241 gcggaccgcg cagctggcga cggccaatcg atctcttcag gacgagatcg ccgagcggaa
     6301 acatgtggag gaggcggtac gcgacagcga attgcggtac aagctgctga cggaggcgac
     6361 gttcgacggc atcgccatcc acaatcaggg gatcctgatc gaggtgaatg cgggtttggg
     6421 gcggatattc gggtacggac cgggcgagct catcggccga tccctgtggg atctcgtcgc
     6481 cgacgaatcc cgcgatctgg tcctggccaa tatgcagaac ggcgtgaacg ggccctacga
     6541 agccatgggg cgtcgcaaag acggcaccac ttttcccggt gaagtcgtag tccggccctg
     6601 tcgttatcgt gggaaagaag ttcgacttgt ggcgggccgt gacattactg agcgcaagca
     6661 cttggaggcg gagcggtccc gccatacgga ggagttggag catcagatcg ccgaacggac
     6721 cgccgagatc gccaagttgg aatcacagcg tgcgcagaca gaaaaactgg cggcaatggg
     6781 ccgcttagcc gccggcgttg cacatgaaat caataacccg attgccggga tcaagaacgc
     6841 ctttacgctc gtgaagcaag cggtagatcc cgcccatcct cacgccgaat tcgtcggcat
     6901 gatcgatcgt gagatcactc gcgtcgcatc catcgtccag aacatgtatc aactctatcg
     6961 gccggagtcc ggcaaagggg aggcggtgga actccgatcg atgatgaacg acatcgaggc
     7021 gcttttctcc aaacggctgc aacagcgccg gcttgagctg gcgatcgatg tggatccctg
     7081 tatcgaccgg ctccgcgtac cgcgaaggga tttgctgcaa gtcctgttga atctgctgaa
     7141 taatgccatc gattgttcca gcgacggagg tacggtcaca ctggccattt gtgtggaacc
     7201 ggatgcgata cgaattgccg tgtccgacca agggccgggc atttccccgg acaatctgcc
     7261 gcacatcttc gacccattct ttacgacaaa gacggagaga gaccagaggg gcatggggct
     7321 ggggctttcc atctctcaga gcctggtgat ggcaatgggc ggcaagatcg aggtccggac
     7381 tcagccgaac ggcgggtcga cgttttcagt cctcttaccg cgtcacacgg ctggtgcatg
     7441 tgcgcaagat cagtcgaata tcataaagga ggttgtcact catgctgact gagacaccgc
     7501 ggattctact cgccgacgat gaagaaacgt tccgtcgctc gacggcgaag cttttggagc
     7561 aagagggata tcgctgtgat tgcgcgcagg actctcatga agccggtatg ctcctgacga
     7621 gacaatatga tgccctcatt accgatattc gcatgccggg caatatgcaa ttcgaatttc
     7681 tccgggatgt ccgtacccgg ttcccttcgc ttccgatcgt gctcgtgacc ggttatccct
     7741 cggttcaaac cgccgtggaa gcactccgcc tatcgttcac ggattatcta ctcaagccgg
     7801 tggactggcc ggatttgctt cgtgcggtgg gcgaagcggt gaagaggacc agtcttttgc
     7861 gcatgaccac agcggttcga gaagaagcga gtcgcctggt tgcttccttg gagcagttac
     7921 aagatgactt gccccgactg ggtagcgggg ccaacgaacg ggaactggca tggtcgctgg
     7981 atgcgtttct ttcccagagt ttcgcacaga tggccgtctt gtcttccaga atccgatccg
     8041 ttctgacgag tcaattgcaa ggcggtgccg aggggcctat ggatgtttgc cgcatgatgc
     8101 aatgtccgag actcgatgcg tatcgggaag cacttgaaag tacggttgag gtgctggcaa
     8161 acaccaagca cgcatctcga tcccaggacc tgggactgtt acggaataaa atcgaacagt
     8221 tactccgcga ggatgcatga attcataatc gccgtcaagt ccatccacca cagggccgac
     8281 atcgtggccg gtccggggct cgctcgccaa gggtcgatcc tcacgaatgt agggatcgcg
     8341 tcggcggtgt tctttgccga accgatggat cctgccgagc aacccttccc gaagcactct
     8401 ttcccatggc ctctccgttt ttctgactgc gggtgtgtct tccgccgccg cttaaggttt
     8461 cccattccga accgatagag tgtcagttaa ggcataggat gccggtcgag cgaacggctc
     8521 aaaccgcttt ggcggatggg agagagacct gccatttgaa gaccacttca atgacactcg
     8581 aaacagagtc ggtatgggtg ccgtggcaga atggaccggc gccgcactgt ggacgggttc
     8641 gaagagtttt aacacggcga ctggagccgg tttgtacgga acaaacgtga ggaattgtct
     8701 caaccagccg gatgatactc agtgagcgga tgccgtaaag gaggagtatc gtgaatactc
     8761 tgcggaatct caagaccagg cccaagttga tgatcagttt cgggctcgtg ggcttgctgt
     8821 tgttaacggt tggaatactg tcgatcaacg gcatcgacaa gttgaacgag cgaatgaacg
     8881 tgatctacaa ggtgaatctg acctcgctca agctgctggg agacctgcat gggcagactg
     8941 aacggatgag cgcgctcgtg gcttggcaca ttctcgcgta tgactcggcg acggtggcca
     9001 agtggaccca agaaataggc aagcttgacg aggacgttga tcgttttgtg acggaatatc
     9061 agccgctcat catcgctcaa tcggaacgtg agatctatga gcatttcaaa accagctggg
     9121 ctgaatataa agacgtccga gcaaaagtca tgcgcctgag cgcaaatttc agcaaggatg
     9181 gggcggctga gctccagcgg accgagttgg ctgagaaact cactgctatt ctgggagcga
     9241 tcgaaggctt gactcgtgag aacgaaatgc aagccaagga gacgttcgac gtcagtttgg
     9301 acatggcctc tacgcttcat accactaaca ttgtcatcgt gtgtgtcgga ctctctttag
     9361 gactgctcct aggttggctc atttcacgct cggtgtccgg cggactgaac gatattttga
     9421 atgccgcgca agcactgggg gccggcaatc ttgcggcacg atccggcatc acgaccaagg
     9481 atgatatcgg tggcctggcc caggcgttca atcggatggg ggaacagatc gaatcgaacg
     9541 tgaaggagtc gctcgagtcc aaatataaga tggtggcgct ggatagagcg gaaggggtga
     9601 ttgagttcaa tacggacggc accatcatta ccgccaacca gaactttttg aattttttgg
     9661 gctacacgct cgacgaggtt aaaggtcatc accaacggct gttcgccgag cccgcctatg
     9721 cggcgtcggc cgactatcag gccttctggc agagacttaa ccggggcgag tatgacgcgg
     9781 gcgtctaccg gcggttgggc aagggaggca aagaaatttg gattcaggcg tcctacaacc
     9841 cgattcttga cggcgctgga aaagtgtcca agatcgtcgc gttcgctacc gacatcaccg
     9901 gtcaaaagca agcgcagaac gaggtggaaa agctcatcaa agctgccgcg atgggtaatc
     9961 tctcagatcg gatcgatacc gaacggttcc aaggtgcagc gaaggacctt gtatcgagct
    10021 ttaatcaact gctcgatgcc gtcgctacgc cgctgcatga agcgcagggt gt
//
LOCUS       DATNDD010000236         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_414698, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000236 DATNDD010000000
VERSION     DATNDD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414698"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VN647_05690"
     CDS             <1..549
                     /locus_tag="VN647_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG96556.1"
                     /translation="GYLAHAVLPSVSVLYMGYRYGWGTAGVGLMMAGVGMAATIVQGG
                     LIRPITNRFGERRTVLTGLLCGALGFFVYGMAPEGWIFCLGIPVMAFWGLAGPATQSL
                     MTRRVSSAEQGQLQGAIAGINGMTGMIGPILFTQTFAYFIRSDSAAGRSIELPGAPFI
                     LASLMLLGAAVIAWRTTKAGGP"
     gene            950..1150
                     /locus_tag="VN647_05695"
     CDS             950..1150
                     /locus_tag="VN647_05695"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWG96557.1"
                     /translation="MKLQVVVEQDEAGYFVAEVPALPGCLSQGKTHDEAIANVKEAIE
                     GWLEVMEAKRVVDAERTVEVVV"
     gene            1276..1518
                     /locus_tag="VN647_05700"
     CDS             1276..1518
                     /locus_tag="VN647_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HWG96558.1"
                     /translation="MKSCPFCKAPVKRRTIEHVHRWGKRLFLFKNVQAEVCSQCRETF
                     FKPAVLRLMDRCAATGKVGRARVSIPVISLPDKVSA"
     gene            1728..1907
                     /locus_tag="VN647_05705"
     CDS             1728..1907
                     /locus_tag="VN647_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWG96559.1"
                     /translation="MAARGLKQKAAARTLGVTQPRVSDLLRGRIDLFTTDALIDMLTR
                     LGATVRLTVKVRRAA"
     gene            complement(2022..>3227)
                     /locus_tag="VN647_05710"
     CDS             complement(2022..>3227)
                     /locus_tag="VN647_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96560.1"
                     /translation="TDWVHLGGVSWKEITVIPNPDGRLELFILAGGDAWHMWQIPLTG
                     DHCQPYIGAVNTARQHLTAVLNDPDHTPAEFRAAEQAVTNAENALKQCRLNPPLVGWS
                     GSVSFGAHDLRQIGVARNADGRLELFGLGGDNSIYHIWMTAPNNGWVGQWSNFNFTVA
                     AARQFWVASDATGRLVVLYTRDGSVNIVGQTQPNNGWRGFDSLTDPEPKPTVTLNVAS
                     GFSKEINKGQSTALSWTSEHADSLEINNGVGSVPVASKPLPVKPDITTTYEITARSRG
                     GTATDTQVIIVDQPLPPPPKGFSSVLFNNCTDETLHFWLQDLTAGGQWKEKGTLPSQK
                     DPSGGPCPGGRQPFQVTLPANHEYLIVAVMPSLCGGANDPTNRDCRRYMTQVVGDPKG
                     APVLVPPIS"
BASE COUNT          713 a          849 c          917 g          748 t
ORIGIN      
        1 ggctatctcg cccatgccgt gcttccaagc gtgtcggtcc tgtacatggg ctatcgctac
       61 ggctggggaa cggccggcgt cggactgatg atggccggcg tcggcatggc ggcgacgatc
      121 gtgcagggcg ggctgatccg tcccatcacc aatcggttcg gcgaacgcag gacggtattg
      181 accggattac tctgcggggc tctcggcttt tttgtctacg gcatggcacc ggagggttgg
      241 atcttctgcc tcggcattcc cgtgatggcc ttctggggac tcgccggtcc ggcgacgcaa
      301 tccctcatga cacgccgcgt cagcagtgcg gaacagggcc agcttcaggg cgccatcgcc
      361 ggcatcaacg gaatgaccgg catgatcgga ccgatcctct tcacgcaaac cttcgcctac
      421 ttcatccgat cggattctgc cgccggcagg tccatagaac ttcccggcgc tccgttcatc
      481 ctcgcctcgc tcatgctcct cggcgccgcc gtgatcgcgt ggcgaacgac gaaagccggc
      541 ggaccatagg tatgttgtat ccgttgtttt tcgatgaagg tgaatcagat tcgcacgatg
      601 attgggtaag aggccggaca gcaaccaagt ggaatacgtt gaggtctctg tgtaacaaac
      661 ttcacgggga ctctccgcac ggttgcaaaa cgatcaatcc atacgcgtag caagggagta
      721 gctggtctga cggagattgt aagtttagcc gcgcgtcagc gatctcttcc gtgatttggt
      781 tcttgcactc cacagatcat tactcatgcc taacagtatt gtctcgacat gcacgactca
      841 gggtctgacc ctgattttga tgttcaatac atcaagcggg tttgcttgac catagtgagg
      901 gtcttcacga taatagctca agagttattc tgaagaagga gattaaggca tgaaactcca
      961 ggttgttgtg gaacaggatg aggcaggtta ctttgtcgcc gaggtgcctg ctttgccagg
     1021 gtgtctgtcg caagggaaaa cgcacgacga ggccatcgcc aatgtgaaag aagccatcga
     1081 agggtggctt gaggtgatgg aggccaagcg cgtggtcgat gcagaacgga cggttgaggt
     1141 tgtggtctga tggcggaacg ccttcggttg tgttcggggt cagacgattc acgagaacac
     1201 ggctattctg atcacgatct atcggccaaa ttcgaggctg tggacgaact ggcgaaaaag
     1261 aaaagggagg cgataatgaa gtcctgccca ttctgcaaag ccccagtgaa gcgaagaacg
     1321 atcgaacatg tccatcggtg ggggaaacgt ctgtttctgt ttaagaacgt gcaggctgag
     1381 gtgtgcagtc aatgcaggga gacatttttt aagcctgcgg tccttcgact catggaccga
     1441 tgtgctgcaa caggaaaggt cgggcgagcg cgggtgagta ttcccgtgat tagcttaccg
     1501 gacaaagtca gcgcgtaaca gcgtggtttc ttgagtcggc agattatttc ctgcctccaa
     1561 tcttcgcaca tgccagaggt cggccgctct cattcaaagg ccaagggcta tatgaaatca
     1621 aagaccaccc catcaaccgg aaatgtgttc cgtgacctgg aatttcgtcg cgaggaagcc
     1681 gagcatcttc tggtccgcgc cgatctcatg atccagctac aaagctcatc gcggctcgtg
     1741 gcctcaaaca gaaggctgcc gccaggacac tgggcgtgac gcaaccccgc gtcagtgatc
     1801 ttctccgggg gcgcatcgat ctcttcacta ccgacgcgct tatcgatatg cttacccgcc
     1861 tcggcgcgac cgttcgtctg accgttaaag ttcgccgggc ggcgtgactt ggttctcgca
     1921 ctccatagat cattactcat gcctaacagt attgtctcga tatgcacggt tcaagatctg
     1981 accctgattt ttggattctg tcgtgggacg ctgactgctg attagctgat gggaggaacg
     2041 aggacaggtg ctcccttggg gtcgccaacg acttgtgtca tataccttcg gcagtctcga
     2101 ttagtgggat cattcgcacc gccgcataac gatggcatca cagcaacgat taaatattca
     2161 tgattagcag gcaaggttac ctggaacggc tgcctgccgc ctggacaagg gccaccggac
     2221 gggtcctttt gtgatggaag ggtgcctttt tctttccatt gaccgccagc cgtaagatcc
     2281 tgtagccaga aatgcagcgt ctcgtcggtg caattgttga agagaacgct agaaaaaccc
     2341 tttggcggcg gcggcagcgg ttggtccaca ataatgactt gtgtatctgt ggccgtgcca
     2401 ccgcggctgc gcgctgtaat ttcataggtg gtggtaatgt ccggcttgac gggaaggggc
     2461 ttgctagcca ctggaacaga gccgacgcca ttgttaattt caagcgaatc agcatgctcg
     2521 gatgtccacg ataaggcggt cgattgacct ttgttgattt ccttggaaaa cccggatgcg
     2581 acattcaagg tgaccgttgg tttcggttcg gggtcggtca gagaatcgaa acctctccag
     2641 ccattattgg gctgggtttg gccgacaata tttacacttc cgtccctcgt atagaggacg
     2701 acaagtcgac ctgtcgcgtc ggaagcgacc cagaactgcc gagcagcagc gacagtgaaa
     2761 ttgaaattgc tccattgtcc aacccagcca ttattcggtg ctgtcatcca gatatgataa
     2821 atcgaattgt caccgccgag tccgaataac tctaaccgcc cgtcggcatt ccgcgcgacc
     2881 ccgatttgcc gaaggtcatg ggcgccaaag gaaaccgaac cgctccagcc gactaatgga
     2941 ggatttaatc tacattgttt aagggcattt tcagcgttag taacagcttg ttcagccgcc
     3001 ctaaactcag cgggagtatg atctggatca ttaagaaccg ctgtaaggtg ttgccgcgca
     3061 gtattcactg cacctatata gggttgacag tgatcgcccg tgagcggaat ctgccacata
     3121 tgccaggcat cgccgcccgc cagaatgaac agttccagcc gaccgtctgg attagggatg
     3181 accgtgattt ctttccagga aactcctccc agatgaaccc aatcggt
//
LOCUS       DATNDD010000237         3305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_675546, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000237 DATNDD010000000
VERSION     DATNDD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675546"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /gene="ilvD"
                     /locus_tag="VN647_05715"
     CDS             <1..1435
                     /gene="ilvD"
                     /locus_tag="VN647_05715"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWG96561.1"
                     /translation="AVSDGISMGTEGMKASLISREVIADSIELVARGHLFDAVVALSG
                     CDKTIPGTVMALARLNVPSLMLYGGSIMPGQFQGHDVTIQDVFEAVGKHASGKMTDAE
                     LKDLEDHACPGPGACGGQFTANTMAIAFEFLGISPMGRNGVPAMDGRKDDVAFECGKM
                     VMELVKQDLRPRQIITRKSLENAIAAVATTGGSTNAVLHLLAIARESGIKLSIDDFDK
                     MNRKVPLLADLKPGGRFAAADLYAAGGTILVAKRLLDAGLLHGNQPTVTGRTIGEEAK
                     DAVETPGQQVLRPLSHPIKPTGGLVILKGNLAPEGCVVKVAGHSMTKFQGPAKVFDRE
                     EDAFVAVKAGQIKSGDVVVIRYEGPAGGPGMREMLGVTAAIVGAGLGDSVALLTDGRF
                     SGATHGLMAGHVAPEAAKGGPIAAVKNGDTITFDIPKRRLDVALSQKEIVTRLKKVKP
                     PVPRYTSGVMGKYARHVSSASEGAITS"
     gene            complement(1511..2272)
                     /locus_tag="VN647_05720"
     CDS             complement(1511..2272)
                     /locus_tag="VN647_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG96562.1"
                     /translation="MKVLSIAFNTFRENLRDKLLYNLLIFALLMIGSSLILMRLTLGE
                     FHRLILDIGLGSINFFGVLIAIFVGIGLVSKEIEKKTIYTIVSKPVARYQFLLGKYFG
                     LSLTLVINTAIMAAGLLAVLYFQDVPVHAVLFKALGMIVLEFMVITAVALLFSTFSSA
                     TLSAIFTLAIYVIGHLTTDLKAFGQKMEGVGRSVLEGMYYILPNLDRFNLKGNVTHQL
                     DVPAGDLLMIAAYGLAYTAFLLMLASVIFQQRDFR"
     gene            complement(2269..3237)
                     /locus_tag="VN647_05725"
     CDS             complement(2269..3237)
                     /locus_tag="VN647_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG96563.1"
                     /translation="MDAVPQPVVQIQSFIKVFRVGFWGRRVTAVDGVSLEVRQGEVFG
                     FLGPNGAGKTTTIKMLMGLIYPSGGTATLFGRPIGDPAAKAKVGFLPESPYFYDYLTG
                     REFLRFYGHLFGLLGSTLEKRIDELLELVGMTHTRDLQLRKFSKGMLQRVGIAQALIN
                     DPELVILDEPMSGLDPIGRKEVRDLILRLKETGKTVMFSSHILHDAELLCDRVAMIMK
                     GKLVACGHVSELIAGDMTHEVEMVIDRLLPEALERLKPLADKVVLQGERVMVVLASQQ
                     NVDQALEVIRSAKAKLVALTPHKASLEDLFIREAKGIQSAVEARVS"
BASE COUNT          791 a          929 c          894 g          691 t
ORIGIN      
        1 cgccgtgtct gatgggatct cgatgggtac cgaagggatg aaagcttccc tcatcagccg
       61 tgaggtcatt gccgattcga tcgagcttgt cgcgcgtgga cacttgtttg atgccgtcgt
      121 cgccctgtcc gggtgcgata aaacgattcc cggaaccgtc atggccctcg cccgattgaa
      181 cgtgccgtcg ctcatgctct atgggggctc catcatgccg ggacagtttc agggacatga
      241 cgtgaccatt caggatgtct tcgaagcagt cggcaagcat gcgtcgggaa agatgaccga
      301 cgccgagctg aaggacttgg aggatcacgc atgccccggg cccggcgctt gtggaggcca
      361 gttcaccgcc aataccatgg ccattgcgtt tgagttcctc ggcatttcac cgatgggacg
      421 caatggggtt cccgccatgg acggtcggaa agacgatgtc gcctttgagt gcggcaagat
      481 ggtgatggaa ctcgtcaaac aagatctgcg cccgcgtcag attattaccc gaaagtcgtt
      541 ggagaacgct atcgccgccg tcgccacgac cgggggctcg acaaacgccg tgctgcatct
      601 cctcgccatc gcccgtgagt ccgggatcaa gctgagcatc gacgacttcg acaagatgaa
      661 tcgcaaggtc cccttgctgg ccgacctcaa gccgggaggc cgcttcgcag cggcagacct
      721 ctatgccgca ggcgggacaa tcttagtcgc caaacgatta ctcgatgccg gtctgcttca
      781 cggcaatcag cctaccgtca caggccgaac gatcggcgaa gaagccaagg atgccgtgga
      841 gactcccggt caacaagtct tgcgtcccct ctcccacccc atcaaaccga caggcggcct
      901 tgtcattctg aaaggcaatc tagccccgga aggctgcgtg gtgaaggttg ccggccattc
      961 aatgaccaag tttcagggac cggcaaaagt ctttgatcgg gaggaagatg cgttcgtagc
     1021 ggtcaaggcg ggacaaatta aatcaggtga tgttgtcgtc attcgctatg aaggtccggc
     1081 aggaggacca ggcatgcgcg agatgttggg cgtgacggcg gctattgtgg gcgccggcct
     1141 tggggactcg gtcgcgctgc ttaccgacgg ccgattttct ggagcaacac atggcttgat
     1201 ggcaggacac gtagcccctg aagcggcgaa gggaggaccg attgcggctg tcaagaacgg
     1261 agacacgatt acgttcgata ttcccaaacg tcgcctggac gtagccctct cgcaaaaaga
     1321 gattgtcact cggctgaaga aagtcaaacc gccggtgcca cgctatactt caggcgtgat
     1381 gggaaaatat gccaggcatg tctcatctgc atcggaaggc gctatcacaa gctgacatga
     1441 tgattagctt gaagtaagaa cgaatgatac ctgattgact acgaataaga gaatagcgag
     1501 agatgtgggc ttaccgaaag tcccgctgtt gaaagatgac tgaggcgagc atgaggagaa
     1561 aagccgtata ggccaatcca taggcggcga tcatcagcag atcaccggcc ggcacgtcga
     1621 gttggtgagt cacgttcccc ttgagattga atcgatccag gttcggaagg atatagtaca
     1681 tgccttccaa cacagaccgc cctacacctt ccattttctg accgaacgct ttcaaatccg
     1741 tcgtcagatg gccgatcaca tatatggcga gggtaaagat cgcgctgagc gtcgcgcttg
     1801 agaaagtgga aaacaggagc gccactgctg tgatgaccat gaattccagc acaatcatcc
     1861 ccagcgcctt gaagagcacg gcatgaacag gaacatcctg gaaatacaac accgccaaca
     1921 agcccgccgc catgatcgcc gtattgatca ccagcgtcag actgagtccg aaatacttgc
     1981 ctaagaggaa ctgataccgc gcgacgggct tggacacgat cgtgtagatc gttttcttct
     2041 cgatttcctt gctgaccagg ccgatgccga cgaagatcgc gatcaataca ccgaaaaaat
     2101 tgatgctccc gagcccgata tccaagatca agcgatgaaa ctcccccagc gtcaatcgca
     2161 tcaggatcag agaactgccg atcatcaaaa gcgcgaagat caagagattg tagagcagct
     2221 tgtctcgaag attctcacga aacgtgttga atgcgatcga gaggaccttc atgaaaccct
     2281 cgcctcaacc gccgactgta tccctttggc ttcacgaata aacagatctt cgagcgaggc
     2341 tttgtgcggt gtgagcgcca ccagcttcgc cttggccgac cggatcacct cgagcgcctg
     2401 atccacattc tgctggctcg ccagtaccac catgacccgc tccccttgta gaacgacttt
     2461 gtcggccaag ggtttgagcc gttcgagcgc ttcaggaagc agccggtcga tcaccatttc
     2521 gacttcatgg gtcatgtccc ctgcgatcaa ttccgacaca tgcccgcagg cgaccaactt
     2581 ccccttcatg atcatggcca cacgatcgca gagcagctcg gcatcatgca ggatgtggga
     2641 actgaacatc acggtcttac cggtttcttt gagccgcaga atcaggtcac gtacctcttt
     2701 ccgtccgatc ggatccagtc ccgacatggg ttcatccaag atgaccagct ccggatcatt
     2761 gattaaggcc tgggcgatcc ccacccgttg caacatgcct ttcgaaaact ttcgcaactg
     2821 gagatcacgg gtatgggtca tgccgacaag ttccaacaat tcgtcaatgc gcttttcgag
     2881 cgttgatccc aataatccga agagatgacc gtagaatcgc aagaattctc ggccggtcag
     2941 atagtcataa aaatacggag attcaggaag gaatccgact ttggccttcg cagccgggtc
     3001 gccaattggc cgtccgaaca gggttgctgt tcccccactc ggatagatca gccccatcag
     3061 catcttaatc gttgtcgttt ttccagcgcc gttggggcca agaaacccaa agacttcgcc
     3121 ttgtctcacc tcaagcgata ctccgtcaac cgccgtcacg cgccgacccc aaaagccgac
     3181 gcggaagact tttataaagc tttggatttg aacaacaggt tggggcactg catccatgat
     3241 ttctgagctt ggctatacac tatcctgttt atccagtcgg aataccttga gtcgatcagg
     3301 atggg
//
LOCUS       DATNDD010000238         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4102082, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000238 DATNDD010000000
VERSION     DATNDD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4102082"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VN647_05730"
     CDS             complement(<1..362)
                     /locus_tag="VN647_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96564.1"
                     /translation="MPIGRKSGSDTAHRCLGASFHVMLVGAVLTVVGCTVGPHYKNPV
                     LKVNDSWNVNSPQISAQPVADSAWWKSFNDPTLEKLIQLAYNQNLPLQVAGLRIMEAR
                     AVLGVSVGNQYPQGQISA"
     gene            complement(559..1398)
                     /locus_tag="VN647_05735"
     CDS             complement(559..1398)
                     /locus_tag="VN647_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWG96565.1"
                     /translation="MINSRTGDTLTVTGQGQSITVVLTDDTVTRDRRGLFGLDKTQMS
                     NIVLIPGLKVNVNGTTDAEGRIVAKTITVDGDDLESSEMIQAGLHPTAQKVAANEQLL
                     EAHGQGIAANQQGIAANQQGIAANQQSIAAHQASIEKSMKDIDAHTERFMALADYDTK
                     GEATVKFKSGSSRVSAADLEQLKQLAQTAKGLTGYIIEVTGYADSRGSAAMNTKLSED
                     RAKAVVTALMQQGGIPVRRIVAPGAMGEYGSAAPNETKSGRAENRRVEVKVLVNKGIA
                     AGQ"
     gene            complement(2170..2649)
                     /locus_tag="VN647_05740"
     CDS             complement(2170..2649)
                     /locus_tag="VN647_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96566.1"
                     /translation="MGTSTQLGKIFPVKFWFCKPSTPEDRQVLVDAFTKGKNVGLTKA
                     LEKMPSAGRISTPGLLGYDIAYYRVIPTATGHRIRFVTDRLLAFGEMYYGTRSLDYSL
                     TAGQIDVNDKDKTKSTGMLYPAAELRMNKKTKEMTWELRMNPWKLQNFLDFGEGKPQ"
BASE COUNT          618 a          849 c          788 g          729 t
ORIGIN      
        1 ctcgctgata tctggccctg tggatactga ttgccgacgg atacgccgag cacggcacgg
       61 gcttccatga tgcgcagccc ggcgacctgt agcggcagat tctggttata ggcgagctgg
      121 atcaattttt cgagcgttgg atcgttgaag gatttccacc aggcgctgtc cgcgaccggc
      181 tgagcagaga tttgtgggct gttcacgttc caactgtcgt tgaccttcag taccggattt
      241 ttgtagtgtg ggccgaccgt gcaaccaaca accgtcagca ccgcgcccac gagcataacg
      301 tgaaagctgg cgcccaggca tcgatgagca gtgtcggacc cagatttgcg gccaatcggc
      361 atggcgatcc tcacctttgt ctggctgaca gcgtacatga gactgcaacc cacgccgccg
      421 gggcaatcaa gagatgtcga aagccctact ggcaggcaag tattgaattc catcttgcct
      481 gcttaacttg acatcgattt ccccatgatc gcctggcagc gactggttaa gtcgcgaggg
      541 gcgcttaaaa agcgttccct attgacctgc ggcgatacct ttgttcacca ggaccttaac
      601 ctcaacccga cggttttccg cccggccgga tttagtttcg ttcggtgcgg ctgatccata
      661 ttcgcccatt gcgcccggcg ctacgatgcg tcgtaccggt atgccaccct gctgcatcaa
      721 agccgtgacc accgctttgg cgcggtcctc gctgagtttg gtgttcatcg cagcgctgcc
      781 cctcgagtcg gcatagcccg tcacctcgat gatgtatccg gttaggcctt ttgcggtttg
      841 cgccaactgt ttgagttgtt ccaggtccgc cgccgaaacc cgcgagctgc cgctcttgaa
      901 tttcacggtg gcttcgccct tcgtgtcgta gtcggccagc gccataaacc gttcagtgtg
      961 cgcgtcaatg tccttcatgc tcttttcaat ggaggcttgg tgggcagcga tactctgctg
     1021 gttggcagcg ataccctgtt ggttggcagc gataccctgc tggttggctg caataccttg
     1081 tccatgggcc tcgagaagct gctcgttggc tgcgactttc tgtgcggtgg gatgtaatcc
     1141 agcctgaatc atctctgagc tttccaggtc atcgccatcg acggttatcg tcttcgcgac
     1201 tatgcggccc tcggcatcgg tcgttccatt gacatttact ttgagcccag gtatcagcac
     1261 gatgttggac atttgcgtct tgtccaggcc aaagagacct ctcctatctc tcgttaccgt
     1321 gtcatcggtg aggacaacag taatgctttg cccctggccg gtcacggtca acgtgtcccc
     1381 ggtccgggag ttgatcattc cctgggcctt ggccttttca ccaccctggc caaaagctga
     1441 taccgatgct agtaaacaca acgccagcaa gctcgctata ttcgttttcc gattcctttg
     1501 catagtccca cactccctct cttttttttc gctccgagtg aaacttgccc cgttttctct
     1561 ccccgcaaca tcccagtcct ggcgaatgta ttgggtattg ccctgcgcgg accctagacg
     1621 aggcgtctgg ttgtacacga aggtcgcgga tgttcgtccg tcaccaagtg cgcgatccaa
     1681 aatgaaatcg ctccatcggc gagagtcgag agccaggaac gacaacagaa attggacctc
     1741 aacgccgttg accgatatat tccacctgcg acctgcatgc gacaactagg aaaaccccta
     1801 gcaaggtccc actaaatacc cgccgagttt gacgacagaa cgggtctatc ccgcgcaatt
     1861 catggaagac cagataacat gatttacaaa cgagaggctg aatgaaactc ctctgcgtcc
     1921 ctcctctttt cgctcaagac aggacggacg tcattgtcat gaataatggc gaccggttga
     1981 cgggcgagat aagggtccca acgccgggat gctctatgcc ggcttcgatt acatgctgag
     2041 cacgagctca gtccactggg caatggtgaa tcacagggaa agtaaccata tatttcccgt
     2101 gaaacccgag gatggatgga ccctacgctc cggagcgcgg aagggattac agaaagcgtg
     2161 gaagattcgt tattgtggtt tgccttctcc aaagtcgagg aagttctgca gtttccaggg
     2221 attcattctc agctcccacg tcatctcctt cgtcttctta ttcatcctta gctcggcagc
     2281 tggataaagc atgcccgtgc tcttagtctt gtccttgtcg ttcacatcga tttgaccggc
     2341 agtcaggcta tagtccaatg aacgtgtacc gtagtacatc tcaccaaagg ccagcaagcg
     2401 gtcggtgacg aagcggatcc gatgtccggt cgcggtagga atcactcggt aataggcgat
     2461 atcgtagccg agcaggcccg gcgtcgatat acgacccgcg gaaggcatct tctcgagtgc
     2521 cttggtcagg ccaacgttct tgcccttcgt aaatgcgtcg accagaacct gcctgtcttc
     2581 cggcgttgag ggcttgcaaa accaaaactt gacggggaag atcttaccca gctgggtgct
     2641 ggtacccata gcgtttgcgt ggaacgtttc acagttgtcg gctgcgaatg ctgtggagat
     2701 acccatgacg agtagtatgg tcccgacgaa gaccgcggca gtcatcctgg tacgactgga
     2761 ttgattacgc attttattac ctcactttat ggttatgact gcatcgtccg ggttcacgat
     2821 gaatgtcatc ccccgcacaa aaaacgtcgg tccccgctat tcagccgtaa tctcttccgc
     2881 tggacggact tcagtagaga accgcgaaac ttgagtgccg catggttgga cgtcggcatc
     2941 catttgcagt ctctagagag aatgaggaat accagcgata ccct
//
LOCUS       DATNDD010000239         5420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1467981, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000239 DATNDD010000000
VERSION     DATNDD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467981"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VN647_05745"
     CDS             complement(<1..885)
                     /locus_tag="VN647_05745"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96567.1"
                     /translation="MPRHIAKAFTYLVPPALAQTIAIGRRVLVPFGHTVLDGVVISLS
                     NHLATEIKSASIREIRSLDHDGQASTPPQALFELSRKIAEYYVAPWGQCLRLILPPMA
                     TRQISRARYVATARGRTALEAGLCPDHLKPVLDRIARRTPGILSSTLQPTRQGNTARI
                     DALINKSWIALMPSNHVNVELQKPRRKLTLDEYDRGTPADGMLLAERLPEVAPSWKTL
                     VAHCLHGNHMRKLVLHAPWEHRLSRLVDAIHQAHSMKRATIVLTGEIVRASWLTQVLS
                     RFTDLQIILAHPSSESGQW"
     gene            complement(1093..2667)
                     /locus_tag="VN647_05750"
     CDS             complement(1093..2667)
                     /locus_tag="VN647_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWG96568.1"
                     /translation="MKTLASLVLVLCSGIGISLDEALAQVSEIDAIRFSEAALTFSSG
                     AIQKTSPIDGTVNLVTGDNQSTGNRMLLGKQDSLYLKLNNPTDVAVGDLFTVYRRVRK
                     VFHPVTKEYLGFVMNRSAVVKVTAADHALTTVEAVLSYGPISPGDPVMRFVAPAPDIE
                     SSSATNISDLEGMIVELQADRTMTLVSQSNVVYLDRGKEDGLKVGDLLDIHRHSAGLP
                     PRKIGQLKVLSLESHTATAKIFKANTRVIKGDRFKLVGYSTPTIQSSVGASLESPTAQ
                     ARQTAPADPVSSKLKIQDASGQSRLNLGDLAKFLHFDSGDAAIKPENYKVLDQLIEYL
                     RTSGDMRMIRIEGHTDNVEIGPSLKSRYPSNWELSKVRANGVLRYLVEKGGVEAERIS
                     AVGLGDTKPTSTNTVEEGRTRNRRVEILLYTSETDAPVLKPDVQTQVQRLEHNPSRMS
                     TRESNDQPLLPTAASDTGGSAGRGTLSVGDPSQVSVKDGSDVANTPPDASGAIPTGTN
                     KQDPPPQQPTAGTPTE"
     gene            2835..3029
                     /locus_tag="VN647_05755"
     CDS             2835..3029
                     /locus_tag="VN647_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96569.1"
                     /translation="MNVDEKKDQHLSGSFHQMERIDTQIHRRRKVKAFSPLESEWEAS
                     QAGRSASGDDEQSEEISVDA"
     gene            3217..3600
                     /gene="ndhC"
                     /locus_tag="VN647_05760"
     CDS             3217..3600
                     /gene="ndhC"
                     /locus_tag="VN647_05760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWG96570.1"
                     /translation="MTGTEVLLEYLTKFFPVFLFILVTLTFGVVTLLVSYFVQPRYPE
                     PEKLSTYECGAEPFSDARMPFPVRYYIFAMLFVIFDIEVIFLYPWAVVFTKIGVIGLI
                     EMMIFIGLFIVAYVYAWRKGALEWD"
     gene            3608..4135
                     /locus_tag="VN647_05765"
     CDS             3608..4135
                     /locus_tag="VN647_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWG96571.1"
                     /translation="MGLIQLGRHEKDGAPDVITGSLEKAVNWARKGSLWPMTFGLACC
                     AIEMMAAVSSRYDMDRFGAGVFRGSPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFIPVDIYVPGCPPTPEALLDGILKLQERIMQKR
                     VFVTQPEQVKTALKV"
     gene            4145..4642
                     /locus_tag="VN647_05770"
     CDS             4145..4642
                     /locus_tag="VN647_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWG96572.1"
                     /translation="MHSLLQRIQQTFSVGVTSLAEWRGDAAVTVSRDKLREVARFLRN
                     DPGMDFDYIVHVSSVDWPDDEERFEVVWEFYSIRKRHRIRLKTRVPESDCVVDSLTDL
                     WKGADFMEREVFDMMGIRFRNHPDLRRILMPDDYTEGYPLRKDFPLRGKGWRDTFDFL
                     DEVPR"
     gene            4657..>5420
                     /locus_tag="VN647_05775"
     CDS             4657..>5420
                     /locus_tag="VN647_05775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWG96573.1"
                     /translation="MAFEDQRTTVYKINPEHPESESLPTLRTEELLLNMGPQHPSTHG
                     VLKVILELEGERLVKSTPVMGYLHRGVEKLAEDGTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLLNLQVPERAEYLRTIVAEVQRIIGHQFWLSAQALDIGAMTVFFYCFRDREI
                     LLDWFDELCGARLTTSWYRIGGVERDLTPSLIDKLKQFLNYFPPKIDEYQVFLERNRI
                     WLGRTKGVAVISAEDAVNFGLSGPVLRGSGVDYDLR"
BASE COUNT         1195 a         1333 c         1546 g         1346 t
ORIGIN      
        1 ccattgaccc gattctgaag acgggtgtgc gaggatgatc tggaggtccg tgaatcttga
       61 aagtacttgt gtcaaccacg atgctctcac aatctcgccg gtgagaacga tggtggctct
      121 cttcattgag tgcgcttggt gaatggcgtc gacaagccgg ctgagtcgat gttcccacgg
      181 ggcatgaagt acgagtttcc tcatgtgatt gccgtgaagg cagtgtgcga ccagcgtttt
      241 ccatgagggg gcgacctccg gaagcctttc agcgagcagc atgccgtccg ctggtgttcc
      301 gcgatcatac tcgtccaagg tgagcttcct acgtggcttt tggagctcga cgttgacgtg
      361 gttcgaaggc atgagcgcta tccacgactt gttgatgagg gcgtcgattc gcgcggtatt
      421 tccttgtcga gttggttgaa gtgtcgagga caggattcct ggagttcgtc gggcaattcg
      481 gtcgagaacc ggcttcagat ggtccggaca aagaccggct tccaaggccg tgcgaccccg
      541 cgcagtagcc acgtaccgcg cgcgggagat ttgtcgggtt gccatcgggg gcaatatgag
      601 ccgaagacac tgaccccagg gtgcaacgta gtattctgcg atcttgcgtg atagctcgaa
      661 cagcgcctga ggcggcgtag aagcttgtcc atcgtgatct agagagcgga tctctctaat
      721 ggaagcagac tttatttcgg tcgcaagatg attactcagc gagatgacga ccccgtccag
      781 caccgtatgc ccaaagggga caaggacgcg ccgccctatt gctatggtct gcgctaaggc
      841 gggagggacg agataggtga acgccttcgc gatatggcga ggcacgataa cgtctgcata
      901 gagtgcctcc cttcgacaag gaagggtcga ggtcccatcg gaatccatgt cacattctaa
      961 caatttggct ggcagtctgg aaatgcgaag gacaccagtc acatcggtgt tctcgccaga
     1021 aaggtatcgg ttggacaagg tcgttgagtg gaaaatccaa ataccgatgc aacttcctgc
     1081 cgaagtccga tactattccg tcggcgttcc agcagttggt tgttgaggag gaggatcctg
     1141 tttgttggtt ccggtcggga tagcaccact cgcatcgggg ggggtattgg cgacatctga
     1201 tccgtccttc acagaaacct gggaaggatc gccgacggac aaggtccctc gaccagcgga
     1261 accacctgtg tctgaagcag cagtcggaag gagcggttgg tcattgcttt cacgtgtact
     1321 catcctcgaa ggattgtgtt cgagcctttg cacttgagtc tgcacgtcag gtttaagtac
     1381 cggagcatcg gtttcagaag tatagagtag aatctccacg cgccgattcc tagttcggcc
     1441 ctcctctacc gtattcgtgg aagtcggttt cgtgtcaccg agccccaccg cactgatccg
     1501 ttccgcttcc acaccgccct tttctacgag atagcggagg acgccgtttg cgcgcacttt
     1561 ggacaactcc cagttgcttg gataacgaga tttgagcgaa gggccgatct ccacgttatc
     1621 cgtatgacct tcgattcgga tcatcctcat gtcgccgctg gtgcgcaggt attcaatcaa
     1681 ctgatcgagc actttgtaat tttcaggttt aatggcagca tcccctgagt caaagtgcag
     1741 gaacttcgct agatccccga ggttgagacg actttgtccc gatgcatcct gtatcttcag
     1801 cttgctggat actggatcag ccggggccgt ttgtctggcc tgagccgtgg gcgattccag
     1861 ggaagctcct accgacgact gaatagtggg tgtcgaatat ccgacgagct tgaaccggtc
     1921 tcctttgatg acgcgggtgt tggccttgaa gattttggcg gttgcggtgt gggattccaa
     1981 cgacagcacc ttaagttggc cgatctttcg tggcggaagc cccgcactgt gacggtgaat
     2041 atcgaggaga tcaccgacct tcagcccgtc ctccttccct cgatccagat acacaacgtt
     2101 tgactgtgaa accagcgtca tcgtccggtc ggcttgaagc tcaacgatca tgccttcgag
     2161 gtcagaaatg tttgttgcgg agctcgactc gatgtccgga gccggagcca caaagcgcat
     2221 aacagggtcc ccaggagaaa tcggtccgta actgagcacg gcctctactg tcgtgagagc
     2281 atggtcggcg gcagttacct tcacgaccgc cgaacgattc atgacaaacc cgaggtattc
     2341 cttggtcaca ggatgaaaca ccttccgaac ccgccggtag acggtaaaaa gatcaccaac
     2401 tgcaacatca gtggggttgt tgagcttgag atagagcgaa tcttgcttac caagaagcat
     2461 gcgattgcct gttgactgat tatcacctgt tactaagttt acggttccgt cgattggaga
     2521 tgttttctgg atggcaccgc tggaaaaggt gagcgcagct tctgaaaacc gaatggcatc
     2581 gatctctgac acctgtgcta gtgcttcgtc gagtgagatg ccgattcctg agcacaggac
     2641 taagacgagg gacgccaagg ttttcatagg aagattcctt ccgaggctag tgttgcttga
     2701 aaaaacatag caaaagacgt tgcgttgtca agaatttgag ctgaattggt cggaatttga
     2761 cgggccggtc tttccagtgg taatgtcgga tcttgccatc ccctaataat gatttcaggg
     2821 atcatgacag accgatgaat gttgacgaaa aaaaagatca acatctctcc gggagcttcc
     2881 accagatgga acgaatcgat acacagatcc atcggcgacg gaaggtcaag gcgttttctc
     2941 ctctcgaatc ggaatgggag gcttcgcagg ccggacgatc cgccagtggg gatgatgaac
     3001 aaagcgaaga gatcagcgtt gatgcctgat cgtgaattgt aataagaacc tagccattca
     3061 acctacccac ctttcgttct caggccgcta gggctttcgg gtcttccgga attaggtcct
     3121 ttgttcgctt gctatcccca agatcagggc actaagattt catgtatccg atctataatg
     3181 ccctttccag gtcaatagct tgcgagagaa aactaaatga ccggaaccga agttcttctt
     3241 gaatatctga cgaagttttt cccggtcttt ctctttatct tggtaacatt gacctttggg
     3301 gtagtgacct tgctcgttag ctattttgtg cagcccaggt atccggaacc agaaaaatta
     3361 tcgacctacg aatgtggcgc cgaaccattt tccgacgccc gtatgccgtt tcccgtcaga
     3421 tattacattt ttgcgatgtt gttcgtcatc tttgatattg aagtgatctt cctctatccg
     3481 tgggctgtcg tattcaccaa gattggggtg attgggctga ttgaaatgat gatttttata
     3541 gggctgttca ttgtggccta cgtgtatgcc tggcgaaaag gtgcgctgga gtgggactga
     3601 ggatggtatg ggactgatcc aactgggacg gcacgagaaa gacggggctc cggacgtcat
     3661 tacggggtct cttgaaaaag ccgtgaactg ggcaaggaag ggttctcttt ggccaatgac
     3721 cttcgggctt gcctgctgcg ccatcgagat gatggccgcc gtttcttccc gatacgacat
     3781 ggatcggttt ggggcaggag tgtttcgtgg ttcgccgcgg cagtcggatt tgatgatcgt
     3841 cgcgggaacc gtgtgccgac gtatggcgcc ggtcattcga aagatttacg atcaaatgcc
     3901 ggaacccaaa tacgtgattg cgatgggctc ctgtgccacc tcagggaaca tctatgacag
     3961 ttatagtgtc gtgcaggggg tcgaccgatt tattcccgtt gacatttatg tacccgggtg
     4021 ccctccgact ccggaggcgc tcttggacgg catcctaaaa ctgcaagagc gcattatgca
     4081 aaagcgtgtg tttgtgactc aaccggagca ggtcaagacc gctctgaagg tctgacgcgc
     4141 cgttatgcat tcactgctcc agcgaattca gcagacgttt tcggtcggag tcaccagctt
     4201 ggctgagtgg cggggcgatg cggcggtgac tgtctcgcga gacaagctcc gtgaagttgc
     4261 tcggtttttg cgcaacgatc ccgggatgga tttcgattat attgtccatg tgagctccgt
     4321 ggattggccc gacgacgaag agcgattcga agtcgtgtgg gagttctact cgattcggaa
     4381 gcggcatcgc attcgactga agacgcgggt acctgaatcg gattgtgtcg ttgattcctt
     4441 gacggatctt tggaaaggtg ccgattttat ggaacgtgaa gtcttcgaca tgatgggcat
     4501 ccggtttcga aatcatcccg atcttcgccg gattttaatg ccggatgatt ataccgaggg
     4561 ctaccctttg cgtaaggatt ttccactgcg gggcaaaggc tggcgagata cgtttgattt
     4621 tctggatgaa gttccacgat agggtgtttc accatcatgg cattcgaaga tcaaagaacc
     4681 accgtataca agatcaaccc ggagcatccg gaaagcgagt cgcttccgac tttgcggacc
     4741 gaggagctcc tgctcaatat ggggcctcaa caccccagta cgcacggtgt ccttaaggtg
     4801 atcctggagt tggaagggga acgattggtg aaatctacgc cggtgatggg atacctccac
     4861 cggggagtgg aaaagcttgc tgaagacggc acgtatcatc agttcattcc tcatacggat
     4921 cggctcgact acgtctgcgc gatgtacaat aactttgcct actgccgtgc tgttgaaaaa
     4981 ctcttgaatt tacaagtccc agagcgggcg gaataccttc ggacaatcgt tgcggaagtt
     5041 cagcgcatca ttgggcatca attttggctg agcgcccagg cgcttgacat cggtgccatg
     5101 accgtctttt tttactgttt tcgggatcgg gaaatcctac tcgattggtt cgacgagctg
     5161 tgcggagctc gtcttacgac gagctggtac cgaatcggcg gggttgagcg ggatttgact
     5221 ccctcgttga tcgacaagct caaacagttc ttgaattact tcccaccgaa gatcgacgaa
     5281 tatcaggtgt ttctcgagag aaaccgtatt tggcttgggc gcaccaaggg ggtcgccgta
     5341 atctccgccg aagatgcggt caattttgga ctgagtggac cggttcttcg cgggtccggc
     5401 gtggactatg atcttcgcaa
//
LOCUS       DATNDD010000240         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3868650, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000240 DATNDD010000000
VERSION     DATNDD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868650"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VN647_05780"
     CDS             <1..472
                     /locus_tag="VN647_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HWG96574.1"
                     /translation="TLMRTKFGLVVERAEDDELIRDFLGLLQGSHADYTIVLRELSRF
                     STADGATNETLREHFLNRDRFDEWAARYRDRLRHERSCDDERCDRMNRVNPKYVLRNY
                     LAQTAIEKAHNKDFSEIDRLFTLLQDPFTEQPGMDAYALPPPNWGKHLAVSCSS"
     gene            567..1289
                     /locus_tag="VN647_05785"
     CDS             567..1289
                     /locus_tag="VN647_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96575.1"
                     /translation="MREVEVIVGGLKIRLAGGSDGHGGGDGPFVMLLHGFGAPGDDLV
                     PLADVIQVPTGTRWIFPEAPLALNMGFGDSRAWWIIDFARIQEDRAAGRIRDLSVEIP
                     QGLALAREGFLAFLKELHRQLPVDYKRTIIGGFSQGAMLTCDAVLHTAYPFAGLVQLS
                     GNLLAQAVWGPLMSKRKGLPVFQSHGTSDDILPHIGAERLRDALIQSDLVVEWRSFRG
                     GHDIPEAVLHRLSGFITKAFKR"
     gene            1286..1411
                     /locus_tag="VN647_05790"
     CDS             1286..1411
                     /locus_tag="VN647_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96576.1"
                     /translation="MNMQPFELIGADGKRLIGDCAAGADRQILFITGFLSKRWGN"
     gene            1485..1652
                     /locus_tag="VN647_05795"
     CDS             1485..1652
                     /locus_tag="VN647_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96577.1"
                     /translation="MLVLIHKECGGPALEESSVGEVCAIPVDRFPFPCFTCLEEILDE
                     SEVRLSEELGI"
     gene            complement(1737..>2174)
                     /locus_tag="VN647_05800"
     CDS             complement(1737..>2174)
                     /locus_tag="VN647_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWG96578.1"
                     /translation="ELQDWEPIKLLTVQVDRLRQWYKPGLLCIGDAAHAMSPVGGVGI
                     NLAIQDAVASANLLWHPLREGLVTEADLAKVQARRIWPTRLTQRAQLIIQDRVIRRVL
                     NSKVSLSPPWVVGLLARFPFLRRIPARLIGLGIRPEHVRTPAS"
BASE COUNT          454 a          621 c          612 g          487 t
ORIGIN      
        1 gacgctcatg cggacgaaat tcggattggt cgttgagcga gcagaagacg acgaactcat
       61 tcgcgacttt ttaggtctcc tccagggcag ccatgcggat tatacgattg tcttgagaga
      121 gttgagtagg ttctccacgg ctgacggcgc aaccaacgaa acgttgcgcg aacacttcct
      181 caaccgtgac cgtttcgacg agtgggcggc gcgctatcgg gatcgactgc ggcatgaacg
      241 cagctgcgac gatgagcgat gtgaccgtat gaatcgtgtg aatcccaagt acgtactgcg
      301 taactatctc gctcagacgg cgattgagaa ggcccataac aaagacttct ccgaaatcga
      361 ccgactcttc actttgctcc aagacccctt taccgagcag cccggcatgg atgcctatgc
      421 gcttcctcca ccgaactggg gcaagcattt ggccgtgagt tgttcatcat aagttaacct
      481 caacctcctt cactctctgg accttctatt gtagagtgtc gcgcgcgggt ccttttgaca
      541 gcatctcttc gtcgctaggg agtgacatgc gggaagttga agttatagtg ggcggattaa
      601 agattcgact cgccggcggc tcagacggtc atggcggcgg ggacggccca ttcgtcatgc
      661 tgctccatgg cttcggcgcc ccgggtgacg atctggtccc gctcgcagat gtgatccaag
      721 ttcccaccgg gacgcgctgg atctttccag aagctccgct cgcgctcaat atgggcttcg
      781 gtgattctcg cgcatggtgg atcattgact tcgcgcgtat ccaggaggat cgcgccgccg
      841 gtcgcattcg tgatctgtcg gtggagattc cacagggcct ggccttggct cgagaaggat
      901 ttctggcatt tctcaaggaa ttacaccgac agctcccggt cgactacaag agaacgatca
      961 tcggtgggtt ttcacaaggg gcgatgctca cctgcgatgc cgtcttgcat acagcctatc
     1021 catttgccgg tctcgtgcag ctctccggga atctcctggc tcaggcggtg tggggcccgc
     1081 tcatgtcgaa acgcaaagga ctgccggtct ttcaaagcca cggcacgtcg gacgacattc
     1141 ttccacatat cggtgcggaa cgtctgcgag acgcactgat tcaatcggat ctcgtcgtcg
     1201 agtggcggag cttccgaggc gggcatgaca ttccggaagc cgtgctacac cgactcagtg
     1261 ggttcatcac gaaggcattc aaaagatgaa catgcagcca ttcgaactca tcggcgccga
     1321 tggaaaacgt ctcatcgggg attgcgccgc tggagccgat cggcagatcc tcttcatcac
     1381 cggctttctt tccaagcgct ggggcaactg attcccggcg accaccgact cagtagtcct
     1441 caacaccttg agcggtttcg ccgactggtg atgagggaag cttgatgctg gtgctaatcc
     1501 acaaagaatg cggcggcccg gcattggaag aatcgtcggt gggcgaagtc tgcgcgattc
     1561 ctgtcgatcg cttccccttt ccttgtttca cctgcctgga agaaattctc gacgaatccg
     1621 aagtgcggtt gtcggaagag ttggggatct gacaggttat agtccctaca atcaccggta
     1681 cattctttct attctcgacc gaccatctga ctctgctcca aaagcaggac gctggtttag
     1741 ctggccggcg ttcgtacatg ttcaggccgg atcccaagcc cgatcaacct ggcagggata
     1801 cggcgaagaa aggggaaccg ggccaggagc ccgacgaccc aaggaggtga cagtgaaacc
     1861 ttgctgttca acacacgtct gatgacacga tcctggatga tgagttgcgc tcgttgagtc
     1921 agccgggtag gccatatacg gcgggcttgc accttggcca aatccgcttc ggtcaccagc
     1981 ccctcgcgca atgggtgcca taacagattt gccgacgcca ccgcatcctg aatcgccagg
     2041 ttgatcccaa ctcctccaac tggcgacatg gcgtgggctg cgtctccgat acagagcagc
     2101 ccaggcttat accattgccg taaccgatcg acttgcacgg tcagcagttt aatcggttcc
     2161 caatcttgca gttc
//
LOCUS       DATNDD010000241         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2574353, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000241 DATNDD010000000
VERSION     DATNDD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574353"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VN647_05805"
     CDS             <1..554
                     /locus_tag="VN647_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96579.1"
                     /translation="IAGVVQGDSTSQNGALTSSNATDFSVERVIPLILKATVDGTYTS
                     KQSLNGRTVYQNNAQPQDTFTTTYDRDYELTPNINAVAGTIPARWLQVKNVSVTVSTT
                     GNITGHSITDLSPAGCTFSGLFKPRAHGNVFDVTITFNGQSGCSNGSATVTGVAFFHA
                     GKLYSAALNSDKTNGVVFIGTKS"
     gene            856..1170
                     /locus_tag="VN647_05810"
     CDS             856..1170
                     /locus_tag="VN647_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96580.1"
                     /translation="MMPKQMLVLCTAAFLGGTFGGVMSTQLLSPKPVEAQKPNGVNAE
                     EFLLLDAKGRARAGLGLDANGEVGLVLRSKDGNHTLTLSPDDPSVIKLVERGGRILWE
                     AP"
     gene            complement(1195..1386)
                     /gene="rpmB"
                     /locus_tag="VN647_05815"
     CDS             complement(1195..1386)
                     /gene="rpmB"
                     /locus_tag="VN647_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWG96581.1"
                     /translation="MALQCEICQKKPVSGNNVSHANNKTRRVFNPNIQTVRAVIGKSH
                     RRIRVCTRCLRSGLVRKAV"
     regulatory      1534..1722
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1750..>2655
                     /locus_tag="VN647_05820"
     CDS             1750..>2655
                     /locus_tag="VN647_05820"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HWG96582.1"
                     /translation="MKKRVQSSRSIQALGIFLLSQWHRYRAVMSMWLLVVLSMFFTQA
                     AECGQGDYGEMKRRQQGILTGMPFMAHVSSRSFVDDAGRNIYLAKPPVRIISLAPSVT
                     EMVFAIGAGDRLVAVTDFCDFPPEAAAKAKIGYSNPNLEAVMALQPDLVLAPKEFLRP
                     DVIAALDQLKVPVFVMAATTIEDILAHLGTLGRMLDHPAAANDLTMNLRRELIDIKSR
                     MQKASPVRVLYVLNSQPLITVGPGSFIDQLIGLAGGVNVAAKSAAPYPRLSMETVLLE
                     DPEVLIFPVGQAEGISESEQRTWRRW"
BASE COUNT          634 a          664 c          736 g          621 t
ORIGIN      
        1 tcatcgcagg tgtagtccaa ggagatagca cctcacaaaa tggagcattg acctcttcaa
       61 acgccacgga cttcagtgtg gaacgggtaa taccattaat actcaaggct acagtcgatg
      121 gtacctatac gtccaagcaa agcttgaatg gcaggactgt ctatcagaac aacgcgcaac
      181 cccaagacac atttacgacg acatacgata gggattatga gttgacccca aatatcaatg
      241 ccgtggcggg gactataccg gcccggtggc tgcaggtgaa aaacgtgtct gttaccgtat
      301 ctaccactgg aaacatcact ggtcactcta ttactgatct ctctcctgct ggatgtacct
      361 tcagcggttt attcaagcct cgagcacatg gaaatgtatt tgatgtgacc atcacattca
      421 atggacagag tggttgcagt aacggaagcg ctactgtgac gggggtcgca ttttttcatg
      481 caggaaaact ttacagcgcc gcattgaata gcgacaaaac gaacggagtt gtctttatcg
      541 gcacgaagtc gtaaccgata gagcgcgaga cgggactttt cttttggcgc cagggtcacg
      601 gggaggatat ccaactcgga agccccgatt tcgtcctggg taacgcacaa tctccacatc
      661 aagaaagccg aaagggatat gaacttccct catctatcgg cgtgatcgca gttttcgatt
      721 cctccccatc aaaaccgaca tgagcgacaa cttgtttgat ccttagcgga tcagttgccg
      781 ttgggtgacg cctcgcataa gatcctccga tgttgcacaa ctgtcgtcac gttgaccttc
      841 acgaaagggt tgatcatgat gcccaaacag atgctcgtgc tctgcacagc ggcgtttctc
      901 ggtgggacat tcggtggtgt gatgagtact caattacttt cgccaaaacc agttgaagcg
      961 cagaaaccga acggggtgaa cgccgaggaa ttcttactat tggatgcaaa agggagagct
     1021 cgagccggtc ttgggttaga tgcaaatggc gaggtcgggc tcgtgctcag gagcaaagac
     1081 ggcaaccata ccctgaccct ctctccggac gatccgtcag tcatcaaatt ggtggagcga
     1141 ggaggtcgga ttctctggga agcgccgtaa cttctgtgtg aagggggaaa accttcagac
     1201 cgctttgcgg actaatccgg aacgcaaaca gcgggtgcac acccgaatac gccgatggct
     1261 cttgccgatc acggcacgaa cggtctggat attaggattg aacactcgtc tggttttatt
     1321 gttcgcgtga ctgacattgt tcccggatac aggcttcttt tggcaaatct cacattgtaa
     1381 cgccacagtc gcactccctt cccactgagt gaatcaaagt ttgcgtgcgg atagtaccat
     1441 agcccactga acgctcacaa gcgtcagacc cttctgcgat ggaggtggaa taaacactga
     1501 gcattattga acttgacaag gtttgatgcc atctcctatt gtgctgtccg ttgtgacttc
     1561 cgagtaaggg aagagggtat gaatccctcg cggtcccgcc gctgtcaatg gggacgaaac
     1621 ctgttatgcc actgttttga agcgtgacgc gcttcagatg ggaaggccgg ggagtagggt
     1681 gaaccatgag ccagaagacc tgctcggaga attgaccgtc gttccctcga ggctggggaa
     1741 cgggtgagga tgaagaagcg ggtgcaatcc tcaaggtcta ttcaggcctt ggggattttt
     1801 cttttgagcc aatggcatcg atatcgcgcc gtcatgagca tgtggctact ggtggtgctg
     1861 tcgatgtttt ttacgcaggc tgctgaatgt gggcaaggag attatggtga gatgaagcga
     1921 cggcaacagg gaatcctcac cggtatgccc tttatggcac atgtctcatc acgatcgttt
     1981 gtcgatgacg ccggaaggaa tatctactta gcgaagccgc ctgtgcggat tatctcctta
     2041 gcccccagcg ttaccgaaat ggtgttcgcc atcggcgccg gagaccggct ggttgccgtc
     2101 acggattttt gtgatttccc gcccgaagcg gcggccaaag caaaaatcgg ctactccaac
     2161 cccaatctcg aagcggttat ggcgcttcaa cccgaccttg tcttggcgcc gaaggaattt
     2221 ctcaggccgg atgttatcgc ggcgttggat caactcaagg tgccggtgtt tgtgatggcc
     2281 gcgacgacga ttgaagatat tcttgctcac ctcggcacac tcgggcggat gttagatcat
     2341 ccggctgccg caaacgattt gacgatgaat ctgagacggg aactcatcga catcaagtca
     2401 cggatgcaga aagcatcgcc ggtccgcgtg ctctatgtac tcaatagcca accgttgatc
     2461 acggtcggcc cggggagttt catcgatcag ttgatcggac tcgccggtgg tgtcaacgtc
     2521 gcggctaaga gtgccgcacc ttacccgcgt ctgagcatgg aaacggtgtt gttggaagac
     2581 cctgaggtat tgatctttcc ggtgggccag gccgaaggaa tttccgaaag tgaacaacgg
     2641 acttggcggc gatgg
//
LOCUS       DATNDD010000242        10342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3625445, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000242 DATNDD010000000
VERSION     DATNDD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625445"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..591)
                     /locus_tag="VN647_05825"
     CDS             complement(91..591)
                     /locus_tag="VN647_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three component ABC system middle component"
                     /protein_id="HWG96583.1"
                     /translation="MSALTQEVLNIQNPALGACLLWRFVCGYVDSHPTRDHVPLPLSF
                     LVLPIVLHEETERFVYGTQKTSGLRAFTAKFGKTENSKQDLLLAIHDRMLVLRHLSME
                     SIRLALATRLLHLNAATLIPLSETQAAAGIPSDVRRMMKSSEKLGWWCGLLTMHEVSI
                     ALKVRF"
     gene            complement(588..1805)
                     /locus_tag="VN647_05830"
     CDS             complement(588..1805)
                     /locus_tag="VN647_05830"
                     /inference="COORDINATES: protein motif:HMM:NF042658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HWG96584.1"
                     /translation="MPKKVKSTSGSEAVPATAAGQALGYSLQFTRLTAMLLIAPDGSS
                     CSLEVLDDVAEQTVDGETKLSQSKSSLTGNPVADRAISLWKTIFNWLEVVKTGLVVPS
                     KTTFELYVSRPVNGQLIDAFHESSSVEDAKEAVAKAREELWGKAPDYAKRATLPHGLG
                     CYVNSVLEADEAHLLPIIVSLRLVCGSGSPQADIEAAIRRHPVSEARVLDIADKMCGW
                     VKRQVDKQLEKGLAAVILRDDFQREYTSYVRSVDRDLILKGLGKKPSKAEKLECLQDN
                     FVQQLDLIERSFDDKLAAISDFLQACRDRAMWSQAGDVHENSFTELKDDLERTWKNMH
                     LAISIEAASKTEIQRGQLLYARCMMHKARIQGMEPPPHFVPGCFHVLSDDLEIGWHPT
                     YQTLLKKPPIKPS"
     gene            complement(1990..3390)
                     /locus_tag="VN647_05835"
                     /pseudo
     CDS             complement(1990..3390)
                     /locus_tag="VN647_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            complement(3284..5752)
                     /locus_tag="VN647_05840"
     CDS             complement(3284..5752)
                     /locus_tag="VN647_05840"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWG96585.1"
                     /translation="MTDLDKKSLSERDICTKFITPAVKQAGWDELTQIREEVGFTKGR
                     IIVRGKLVTRGKAKSADYILYYKSNLPIALIEAKDNSYPVGGGMQQGLGYAATLDIPF
                     VFSSNGDGFVFHDRTGQSDQQESELSLNAFPAPEALWKKYRAWKGLARAQEEIVLQDY
                     HDDSTGKEPRYYQRIAINKTIEAIAKGQNRLLLVMATGTGKTYTAFQIIWRLWKAGRK
                     KRILYLADRNVLIDQTMVNDFRPFGKTMAKLSKSSKTIEQEDGSGGDLPTAVDKKHRR
                     IDTSYEIYLGLYQAITGPEDRQKLYRELSSDFFDLIVVDECHRGSAAEDSAWREILDY
                     FSSATHIGLTATPKETEYVSNIHYFGKPVYTYSLRDGIRDGFLAPYKVVKVHLDVDVE
                     GYRPKDGETDKYGHEIEDRIYNQKDYDRNLVIDERTKRVARKVTEFLKESGDRFQKTI
                     VFCVDVEHAARMRQALINENADLMRQNERYVMRITGDDAEGTAQLGNFIDPEAKYPVI
                     VTTSRLLSTGVDAQTCRLIVLDREVGSMTEFKQIVGRGTRVHEDSKKYYFTLIDFRKA
                     TNHFADPDFDGEPVQIYEPGEDDPVAPPDDVQPLTDTEDPIPPKPDEDEQVVVDPAKP
                     DITIPPGGAQPRKYYIHGDEVSVIAERVEYLDEYGKLVTETLRDYTKKTLRKRYASLT
                     VFLKQWNSAERKQAIIEELEQEGVLLEPLADEVGKDLDPFDLICHVAFDQPPLTRRER
                     AENVRKRDVFTKYGKQARAVLEALLQKYQDEGVTGLDDPQILKISPFDAMGTPIELLK
                     TFGGRPGFEQAVHELQSALYQGAA"
     gene            complement(5909..6724)
                     /locus_tag="VN647_05845"
     CDS             complement(5909..6724)
                     /locus_tag="VN647_05845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWG96586.1"
                     /translation="MIDLLRKTYRRLKSIPLVVIGTITHVSTSKPAVALTFDDGPDPV
                     FTPRLLDVLQQHHAKATFFMVGQAAQRHPDIVKNVAAAGHAIGNHSWDHPSFPLLTGR
                     ERRAQIRACAKAIAPYAQPIFRPPYADQNLLSRLDALWLGYQVITYNVAAIDWLDHDA
                     DWIKDRIMSRLQNGSIILFHDSLFRHGEDRYVDREPMLNAVDTLLKELSGRLSFITVP
                     ELLRQGRPQRQYWHKKTDIDFLNGLKGQYGEEAILKSRVAIPRNKPTKRDKPD"
     gene            complement(6772..7647)
                     /locus_tag="VN647_05850"
     CDS             complement(6772..7647)
                     /locus_tag="VN647_05850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG96587.1"
                     /translation="MKLSVIIPCLNAAGTIATQFEALANQNYSEPWEVIVSDNGSTDD
                     LVNVVKRYVNRLPDLRIVDASDKRGQAHARNIGAKFALGESLVFCDADDEVAPGWLSA
                     MGEALSKYDFVACRIDTNKLSPSWVTKQVKHSQASGLQPKGELAYLPHAGGGTLGVKR
                     RIHETIGGFDESLALCEDTDYCWNIQLKGVTLHFVPEAVMHVRLRDTIMGNYKQAVGW
                     NEYRVLLYKKHLPFGMPKRTWKQGIASWKNFIIWFVKSILHIRDKSYRAVWARRLGAK
                     IGRLKGSIKYRTWAI"
     gene            complement(8001..8744)
                     /locus_tag="VN647_05855"
     CDS             complement(8001..8744)
                     /locus_tag="VN647_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWG96588.1"
                     /translation="MRSTRKMVFENHVAVVTGASQGIGRAIALGLAAKEAMLFLVGRN
                     TAALHEVTELAGKSSPRVVAHATDLVAEGATTIIAEEILQQLGGIDMLIHCAGAYSQG
                     GFQTASIDEFDRLYRTNVRMPYLLTQSLLPMLKKRQGQIAFVNSSKGLQAHANAGQFA
                     ATQHALRAIADSLREEVNSDGIRVFSIYPGRTATPRMEAIFQMEGREYKPELLLQPED
                     IAEVVVNTLMIPLTAEVTNINIRPRIKSY"
     gene            complement(8855..9010)
                     /locus_tag="VN647_05860"
     CDS             complement(8855..9010)
                     /locus_tag="VN647_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96589.1"
                     /translation="MAYRQRLDTPKPSELTYRSAEKPQDHREELGDSMTIIDPQENTA
                     VGLQTEP"
     gene            complement(10019..>10342)
                     /locus_tag="VN647_05865"
     CDS             complement(10019..>10342)
                     /locus_tag="VN647_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWG96590.1"
                     /translation="VAKRYSFNAIQDSHQGRDWDFYVTVHGTIDPMTGMVTDIGALDR
                     LVQDTVIKTFNRQDLRQVLESETVRGEALTKTIWDRLAGRLSGGTLRNVRLVQTRDLS
                     FDYAG"
BASE COUNT         2263 a         2827 c         2710 g         2542 t
ORIGIN      
        1 gttactttcc caggctcgaa cttaaccctg cgagggacaa acgcatggtt tctaggccaa
       61 aggataattt ccttaacttg aaagaacatt tcagaatctc actttaagtg cgatgctgac
      121 ctcgtgcatg gtcaacagac cgcaccacca tccgagcttc tcagaacttt tcatcattcg
      181 tctcacatca gatggaatac cggcagcagc ttgggtttct gacaaaggga ttaatgtggc
      241 agcattaaga tgcagtaatc gggtagccaa cgctagacgg atcgattcca tgctgagatg
      301 tcgcaaaacc aacatccgat cgtgaatcgc caaaagcaaa tcttgttttg aattttctgt
      361 tttcccaaac ttagccgtga aagctcttag ccctgaagtc ttctgagttc cataaacaaa
      421 cctctcagtt tcctcatgga gaacgatggg caaaactaga aaagaaagtg gtaagggcac
      481 atggtcgcgg gttggatggc tatcaacata gccacataca aaacgccaca gcaagcacgc
      541 gcccagcgcg gggttctgga tgtttagcac ttcttgtgtt aaagcgctca tgatggcttg
      601 ataggtggct tttttagtag cgtctgatag gtcggatgcc agccgatttc cagatcgtca
      661 gacaagacat ggaaacaacc aggcacaaaa tgtggcggtg gttccatccc ttgaatccga
      721 gctttgtgca tcatacatcg cgcgtataga agctgtcccc gttggatttc ggtttttgat
      781 gcagcctcta tgctaatggc gagatgcata ttcttccatg tccgctcaag atcgtcttta
      841 agctctgtga atgaattttc atgaacgtca ccggcttggc tccacattgc tctatcccga
      901 catgcctgaa gaaagtcgct aatggccgcc agcttatcat cgaaacttcg ttcgataaga
      961 tcgagttgtt gaacgaagtt atcttgcaaa cattctaact tttctgcctt agatggtttt
     1021 ttccccaggc ccttcaggat gaggtctcgg tcgacgctgc ggacatatga ggtatattcg
     1081 cgttgaaaat cgtctcgcag aatgactgct gccaaccctt tctccagttg cttatctacc
     1141 tgccgcttga cccaaccaca catcttgtca gcaatgtcta agactcttgc ctctgataca
     1201 ggatggcgac gaattgcggc ttcaatgtct gcttgtgggc tgccgctgcc gcatacgagt
     1261 cgtaggctaa cgatgatggg caaaagatgt gcttcatcgg cttccaaaac cgaattgaca
     1321 tagcagccaa ggccgtgtgg caaagtggca cgtttagcat agtcaggtgc ctttccccac
     1381 aactcctcgc gcgccttcgc aacagcctct tttgcatctt cgacagaact cgactcgtga
     1441 aaagcgtcta ttaactggcc gtttacaggt cgtgacacat ataactcaaa cgtggtctta
     1501 ctcggtacaa cgaggccggt tttgactacc tctagccaat tgaagatagt tttccatagc
     1561 gaaatggctc gatcggcgac cggattaccc gttaatgaac tcttggactg gctaagctta
     1621 gtttcgccat ctacagtttg ttccgcaaca tcatccaaaa cctccaagct acacgaactc
     1681 ccatctggag ctatgagcag catggcagtc aagcgagtaa attgaaggct gtaaccaagt
     1741 gcctgtcctg ctgccgttgc tggaactgcc tctgacccac tagtcgactt tacttttttc
     1801 ggcatacatt tgcatcccta ccgacaaaca aggaccttct attttcccct ccaccgtacg
     1861 attcgccgat atggaagcac tagatgttta gtcccagcat ttgatgggtt gggtgaacgg
     1921 tgaatcggtc gggttcttgg gtccatattt tgccgatgta ttcaaagggc gtgaggcgcg
     1981 ggttttcacc tcatcagccg tcaccttcca ggcgacttca gactccttgc ggcctttgcg
     2041 tttggccccg ccccaccagt cgatgcaggg cttcagatgc tcgaagcgga tgggcttcgt
     2101 catcgagtag gccttctggc cgtcagggat gcgatgttcg tagaaccaga tgtccttcgt
     2161 cggctcgccc ttctcgaaga acagcagatt cgttccgatg ctggcgtaag gtttgaacac
     2221 gctattgggc agacgaacga tcgtgtggag attgcactcc tccatcaggt gttccttgag
     2281 tctggtcttc accccttcgc cgaacagcga accatccggc aagacaacgg cagcacggcc
     2341 acctgtcttg agcaagcgca caatcagcgc gagaaagaga tccgccgtct cgcgcgtctg
     2401 gaaatgtttt gggaagttgc tttcgatccc gtcttcttcc ttgccgccga agggtggatt
     2461 ggtaaggacg atatccacgc gatcggcttg gccatagctg atatagggcc gggccagagt
     2521 gttgtcgtga cggacgaagg atgggtcttc gatgttgtgc agcagcatgt tcgtgacgca
     2581 gagcatgtgc gggagccgct tcttctcgca ggcccgcaag ccctgttgca tcttctgctt
     2641 atctgccggt ttcttcacat agtgcttact catatgccga atggcacagg tgagaaagcc
     2701 gccggtgccg caggccgggt cgaagagcat ctcgccgggg tgcgggtcga ttcgatccgc
     2761 catgaaggcg gtgacggcgc ggggcgtata gtactcgccc gcgttgccgg cgctctggag
     2821 gtcgttcagg atctgctcgt agatatcgcc gaagtgctgg cgttcggtca ggttgttgaa
     2881 gtcgatgctg ttgatcttat tgaccacctg ccgcatgagc tggccggact ttatgtagtt
     2941 gtaggcgtct tcgaagacat cccgcacgac acggcggcgc ccgtccttgg tcgtgcccga
     3001 cagttccttg agcgagggga agagttcacc attgacgaag gccatgagcg gttcgccggt
     3061 ggtgccttcg ggattggccg cccaggtgcg ccactgaaac ttcttcggaa tgggcgaacg
     3121 gtagtccttc tgcatcgctt cgagttccag atcctggtcg tcgatgatct tcaggaagaa
     3181 catccaacag agctgggaga tgcgctgagc gtcgccatcg acgccgctgt cctggcgcat
     3241 gatgtcttga atagatttga cggtgttgcg aacagacata actttaagcc gctccctggt
     3301 atagcgcaga ttggagttcg tggacggcct gttcaaagcc gggccgtccg ccgaaggtct
     3361 tgagcagctc gatcggcgtg cccatggcat cgaacggcga aatcttgagg atctgcggat
     3421 cgtccaggcc ggtgacgcct tcgtcctgat atttctggag caaggcttcc aacacggcgc
     3481 gggcctgttt accgtatttg gtgaagacat cgcgcttacg gacattctcc gcccgttctc
     3541 ttctcgtgag cggcggctga tcgaaggcca catggcagat gagatcgaag ggatcgagat
     3601 ccttaccgac ttcgtcggcc agtggttcga gcaatacccc ttcttgttcc agctcctcga
     3661 tgatggcttg cttccgttcg gcgctgttcc actgtttcag aaacaccgtg aggctggcat
     3721 agcgtttccg gagcgtcttc ttggtgtagt cgcgtagggt ttccgtcacg agcttgccgt
     3781 actcgtcgag atactcgacc cgctccgcga tgacgctcac ttcatcacca tgaatgtagt
     3841 acttgcgcgg ttgagccccg ccgggcggta tggtgatgtc cggcttggct ggatcaacga
     3901 cgacttgctc atcctcatct ggtttcggtg ggatgggatc ttctgtatca gtcaatggct
     3961 gcacatcgtc cggcggtgcc accgggtcat cttcgcccgg ctcgtagatc tgcaccggct
     4021 cgccgtcgaa gtcggggtcg gcgaagtggt tggtcgcctt ccggaagtcg atgagggtga
     4081 aatagtactt cttgctgtcc tcatgcaccc gagtcccgcg cccgacgatc tgcttgaact
     4141 cggtcatgga accgacttcg cggtcgagca cgatgaggcg gcaggtttgg gcatccactc
     4201 cggtagagag cagccgcgag gtcgtaacga tcaccgggta cttggcctcc ggatcgatga
     4261 agttgccgag ctgcgcggtg ccttcggcat catcgccggt gatgcgcatg acgtagcgtt
     4321 cgttctgccg catcagatcg gcattctcat tgatcagcgc ctggcgcatg cgggcggcgt
     4381 gttcgacgtc cacacagaag acgatggtct tctggaaccg gtcgccgctt tctttcagaa
     4441 actccgtcac cttgcgggcc acgcgctttg tccgctcgtc gatgaccagg tttcgatcgt
     4501 agtctttctg attatagatg cgatcttcga tctcgtgccc atacttgtcg gtttctccat
     4561 ccttgggccg gtagccttcg acatcgacgt ccagatggac ctttaccact ttgtacggag
     4621 ccaggaagcc gtctcgaata ccgtcgcgca gagagtaggt atagaccggt ttgccgaagt
     4681 agtggatgtt ggacacgtac tccgtttcct tcggcgtcgc cgtaaggccg atgtgcgtcg
     4741 ccgacgaaaa gtagtcgaga atctctcgcc acgcagaatc ctccgcggca ctcccccgat
     4801 ggcactcatc gacgacgatc aggtcaaaaa aatccgacga cagttcgcgg tagagtttct
     4861 ggcgatcctc aggccctgtg atagcctgat agaggcccag atagatctca taagacgtgt
     4921 cgatccgccg gtgcttcttg tccacggcgg tcggcaaatc tcccccgctc ccgtcttcct
     4981 gttcgatggt cttggaactc ttgctgagct tggccattgt tttcccgaat ggacggaaat
     5041 cgttgaccat ggtctggtcg atcagtacat tgcggtcggc cagatacaaa atgcgtttct
     5101 tgcgccccgc cttccagaga cgccagatga tttggaaggc ggtgtatgtc ttgccggtgc
     5161 cggtcgccat gaccaggagc aggcgattct gccctttggc gatagcttcg atggtcttgt
     5221 tgattgcgat gcgctggtag tagcgcggtt ctttgcctgt gctatcgtcg tgatagtcct
     5281 ggagaacgat ctcttcctgg gcgcgtgcca aacctttcca ggcgcggtat ttcttccaga
     5341 gggcttctgg tgccgggaag gcattcagcg acagctctga ttcctgttgg tcgctctggc
     5401 ctgttcggtc atgaaaaacg aagccgtctc cgttggatga gaagacaaat gggatatcca
     5461 acgtagccgc ataccccagc ccctgctgca tgccgccgcc gacgggataa ctgttgtcct
     5521 tcgcctcgat aagagcgata ggaaggttcg acttgtagta gaggatgtag tcggcgctct
     5581 tcgcttttcc tcgcgtcacg agtttgcccc gaacgatgat gcggcctttg gtaaacccga
     5641 cctcttcgcg aatctgagtc agctcatccc accctgcctg cttcacggcc ggcgtgatga
     5701 atttggtgca gatatcgcgt tcgctcagcg atttcttgtc gaggtccgtc acaaagataa
     5761 cctcgtcgat atggaattga gccgccgaag cttacccgtt ttttctggaa gaaaccacgc
     5821 tcccggaagg agggaaaagt attgccttcg acaagatcaa gatgagccac tggatgctga
     5881 acggtgttga cttgcctgag ccggatcttt aatccggttt atctcgttta gttggtttgt
     5941 ttcgtggaat tgctactcgg cttttgagta tcgcctcttc accatattgc cctttgagcc
     6001 cattcagaaa atcgatgtcg gttttcttgt gccagtattg ccgttgagga cgtccctgtc
     6061 ggagcagctc cggaacggtg atgaagctga ggcgcccgct cagttctttt agaagcgtat
     6121 ccaccgcatt caacatcggt tctcgatcga cataccgatc ctctccatgc cgaaagagcg
     6181 aatcgtggaa aaggatgatg ctgccgtttt gcagacgact cataatcctg tctttgatcc
     6241 aatctgcatc gtggtcaagc caatctatag ccgccacatt atacgtaatg acctgatatc
     6301 caagccacaa cgcgtcgaga cgcgacaata ggttctggtc tgcataggga ggacggaaga
     6361 ttggttgcgc ataaggagca atggcttttg cgcatgcccg gatctgggct cgccgctcgc
     6421 gtccggtaag caacgggaaa gagggatgat cccaggagtg attgccgatc gcatgtccgg
     6481 cggcagccac attcttcaca atatcaggat gacgctgtgc agcctgaccc accatgaaaa
     6541 acgtcgcctt cgcatgatgc tgttgcaaaa cgtcaaggag gcgaggggtg aaaaccgggt
     6601 ccggaccatc gtcaaaagtc agagctacgg ccggctttga tgtggagaca tgcgtgattg
     6661 tcccaataac aacgagggga atcgatttta atcgtctata tgttttcctt agtaaatcta
     6721 tcattgaatg gaaaatacgg tttcgattga acctttagtt ttgccgacga atcaaatcgc
     6781 ccaagttctg tatttgatgc tccctttcaa acgacctatc ttcgccccca atcgccgagc
     6841 ccaaacggcg cgatagcttt tatctcgaat gtgcagaata ctttttacaa accatataat
     6901 gaaattcttc catgaagcga ttccctgttt ccaagtccgt tttggcatcc cgaagggaag
     6961 atgtttctta tacaggagaa cacgatattc gttccatcca acagcctgct tatagttgcc
     7021 cataattgta tcccttaagc gaacatgcat cactgcctcc ggcacaaaat gaagcgtcac
     7081 acctttcagc tgaatgttcc agcaataatc tgtatcttcg cacaaggcaa gagactcatc
     7141 aaaaccgccg attgtttcat ggatccgacg tttcactcca agagtgcctc ctccagcgtg
     7201 cggaagatag gcaagctccc ctttcggctg aagccctgat gcttgggaat gctttacctg
     7261 cttcgtcacc caggaagggc tgagcttatt cgtatcgata cggcaggcga caaaatcgta
     7321 cttggacaac gcctctccca tggcagaaag ccaacccggg gcgacctcat catctgcatc
     7381 acaaaaaaca agcgattccc ccagcgcgaa cttcgcgcca atattcctgg catgcgcttg
     7441 tccacgcttg tcagatgcgt caacgatacg aagatccgga agacggttca catatctctt
     7501 cacgacgttc actaaatcat ccgtcgatcc gttgtctgaa acaatcactt cccaaggctc
     7561 ggaataattc tgattcgcaa gggcttcgaa ttgagtggca atggtacccg ccgcattcaa
     7621 acaggggatg atgacgctca atttcatccg ttacctcccg cctcaatatc ggataaacaa
     7681 cttaccctgc cggggttcag aggataaccg gccttcgagc atcgatgaca aggcactttc
     7741 ccggcactcg ataattgtcg atctccgatg actgaaacga aggacacatt gtatctagtt
     7801 tcggcctggc tggagaggaa atgaaagagt ttcgcaaatg gaaaggtact gtctggcaga
     7861 cctgaacgaa aagagggtcg agacggaaat ccattgctca tcttttggac acctggtcaa
     7921 acatcgggag gaagctaggg gttctcttcc ctcttagaga ggaaccggca ttacagatat
     7981 cccagccgtc cacttcagca tcaatacgac tttattcgcg gacgaatatt gatgttcgtc
     8041 acctcggctg ttaatggtat catcagagta ttaacgacga cctcagcgat atcttcaggt
     8101 tggagaagaa gttccggttt gtactccctt ccctccatct gaaaaatggc ctccatgcgc
     8161 ggcgtggctg ttcgcccggg atagatacta aacactcgaa tcccatccga attgacctcc
     8221 tccctgagac tgtccgctat agctctcaag gcatgctgcg tcgcggcaaa ctgaccagca
     8281 ttcgcatgcg cctgaagtcc cttactggaa ttgacaaaag caatctggcc ttgccttttt
     8341 ttgagcattg ggagaagtga ctgggttaac agatacggca tacgcacatt ggttcgatac
     8401 aatctatcga attcatcgat cgaagcggtt tgaaagccgc cctgagaata ggctccagca
     8461 cagtgaatta gcatatcaat accgcccagt tgctgaagaa tctcctccgc tataattgtg
     8521 gttgcccctt cagctaccag gtcagtggca tgggccacga cacgcggcga cgattttccg
     8581 gcaagttccg tcacttcgtg taaggcggcg gtatttcgcc caaccaggaa cagcatggct
     8641 tccttagcgg caagacccag agcaatcgcc cgcccgatcc cctgactagc ccccgtaaca
     8701 acggcaacat gattctcgaa gaccatctta cgagtcgatc tcattgcgca acaacctccg
     8761 gtgtgagcat tcaggtcata tatcggtaga ttggatcggc tccgtgagcc aggttcggtt
     8821 tcacgttata tgggattccc ctggcacagt attcttatgg ctcggtctga agaccgaccg
     8881 ccgtattttc ctgtggatca atgatggtca tgctgtcccc tagttcctcc ctatgatcct
     8941 gaggcttttc cgccgaccgg taggtcaact cggaaggctt cggcgtgtcc agcctttggc
     9001 gataagccat gatgtaatca cgaacttgcc gctataataa gcttgggcac gatctaggat
     9061 cgtccaggca agtaggctcg ccgctcggtc gttcgacata ctattcaagt attctccttt
     9121 cttcacggtt ccgtgcgctt gtatcgcgtg acgactgcgc gatctcgcaa caaaccgact
     9181 gggaaaaatg gcctaggtga caccgattat gcaaaacagg ccccgatcgc tcagctgctg
     9241 ctgctccacc acgctccaca agaatcctct acagatcgct gcgtttccgt agcggtctgg
     9301 caagcctcag cagcttcttc ccgtctgcag gacacgcaag acgagcgccc tgtgcaataa
     9361 ttaaatatgg aactaggcaa aacccttgcc gagaaataca agtacaatca cgccgttcta
     9421 ctccaatcgc cgctcgctga acaccgtcaa gacggcgcac tggcacggat gaaccgcagc
     9481 gaggcactgg agacgctcag tccgaacatg tcctcattgc ttcggcacaa gcctcatact
     9541 ggctgcaact caggccctgc aacagtgccg aacagcccta catccgctgc ctggggttcg
     9601 ccaaggctta taaatgtgca attccttacc tcatgttcct aagccggtcc tagacgcggt
     9661 gtaggtggat ggacagcttg atccagcaga tagatcgaac cgggtggccg ccatcagcgc
     9721 acgtattccg gtggctaccc gtccggcgat atcgttcgct gccagcggga tcaccgcctg
     9781 aatcacgccc cccgcacact cggtcgccca ccgccgggaa cctttcaggg ctggccgacc
     9841 gaccgatccg gctttgtctt cgttggtgat tcggattatg tctttatgaa gcggcgtctt
     9901 cgtcaggctg ggtctccacc ggttgcaaca acccgatggg aacattggag gagcattcag
     9961 gcttggacgc tgagggcagc tgaggcagaa aggaagcgag attatagagg tcgtccggtt
    10021 agcccgcgta gtcgaacgaa aggtcgcggg tttggacgag gcggacattg cgaagggttc
    10081 caccggagag acgaccggcg agacgatccc agatggtctt ggtgagcgct tctcccctca
    10141 ctgtttcgga ttcgagcacc tgacggaggt cctgtcgatt gaaagtcttg atgacggtgt
    10201 cctgcaccag tcggtccaag gcaccgatgt cggtcaccat gcccgtcatc ggatcgattg
    10261 ttccatgaac cgtcacataa aagtcccagt cccgcccctg atggctgtct tggatggcat
    10321 tgaacgaata tcgcttggcg ac
//
LOCUS       DATNDD010000243         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1770031, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000243 DATNDD010000000
VERSION     DATNDD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770031"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VN647_05870"
     CDS             <1..966
                     /locus_tag="VN647_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWG96591.1"
                     /translation="TAKVPGGSNAVARYLLPVGAHIFVEKGATVYPGDVLAKIPRETT
                     KTKDITGGLPRVVELFEARKPKEQAVITEIDGEVSYGGFVKGQRKVLVDNKMGDVKEY
                     FIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPNELQKYLVDEVQDVYRLQGV
                     SINDKHIEIIVRQMLRKVRVEDPGDTQFLPGSQVSKSVFEKENERVFANDGKPALGKP
                     VLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKENVIVGRLIPAGSG
                     FEEYRDTFVISEKSEGTPVGVSPAAPAVPSDAAVVPAVSGETARS"
     gene            1075..1446
                     /gene="rpsL"
                     /locus_tag="VN647_05875"
     CDS             1075..1446
                     /gene="rpsL"
                     /locus_tag="VN647_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWG96592.1"
                     /translation="MPTINQLVRKGRIFVKAKTKSPALKSCPQKRGVCLRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHLVRGAL
                     DAVGVADRKQSRSKYGAKRPK"
     gene            1491..1961
                     /gene="rpsG"
                     /locus_tag="VN647_05880"
     CDS             1491..1961
                     /gene="rpsG"
                     /locus_tag="VN647_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWG96593.1"
                     /translation="MPRSRFLGQREVLPDVRYRDKLVGKFINTLMSNGKKSTTERICY
                     GAFDVIQEKTGGDPLKVFKAAVDNVKPIVEVKSRRVGGASYQVPVEIRPARRVSLALR
                     WLSQFARTRGGKSMREKLAAELLDASNNTGAAVKKREDVHRMAEANKAFAHYRW"
     gene            2121..>2887
                     /locus_tag="VN647_05885"
     CDS             2121..>2887
                     /locus_tag="VN647_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWG96594.1"
                     /translation="MAHIDAGKTTTTERILYYTGMTHKMGEVHEGAATMDWMEQERER
                     GITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGAVAAFDSVQGVEPQSE
                     TVWRQADKYHVPRIAFMNKMDRIGADFYGSVQSIIDRLGAKPVPIQIPIGREAEFRGS
                     IDLVRMKGYFYDDETLGAKYKVDEIPGDLLAQAKEYREKMLDAVAEFDDQVMEKYLNG
                     HPLTEEEVMRAIRAGTISMKVTPVLCGSAFKNKGVQQ"
BASE COUNT          714 a          645 c          875 g          653 t
ORIGIN      
        1 acggccaagg tgcccggcgg atccaatgcc gtggccaggt acttattacc ggtcggagct
       61 catatcttcg tcgaaaaagg ggccacggtc tatcccggtg atgtcttggc aaagattcct
      121 cgtgaaacga ctaagaccaa ggacatcacg ggaggtctac cgcgcgtggt ggaacttttc
      181 gaggcaagga aaccgaaaga acaggcggtc atcaccgaga tcgacggaga agtttcctac
      241 ggcggtttcg tgaaaggcca acgcaaagtc cttgtcgaca ataagatggg tgatgtgaag
      301 gaatatttta ttcccaaagg caagcatgtc aatgtccatg aaggggactg ggtgcgggct
      361 ggggagccgt tgatggacgg atcagcgaat ccgcatgaca tccttgatgt gttgggtccg
      421 aacgaattgc agaagtacct tgtggacgaa gtccaagacg tgtatcgatt gcaaggagtt
      481 tcgattaacg acaagcacat cgagatcatc gtgcggcaaa tgttgcggaa ggtacgggtc
      541 gaagatccgg gagacaccca gttcttgccg ggcagtcaag tcagcaagag cgtctttgaa
      601 aaagaaaacg aacgggtatt cgccaacgac gggaagccgg ccctgggcaa gcccgtcttg
      661 cttgggatta cgaaggcggc tcttacgaca gacagcttta tttctgcagc atcgttccag
      721 gaaaccaccc gcgttctcac tgaagcagcg atcaatggac gcgaagataa tttgctgggc
      781 ttaaaagaaa acgtcattgt aggccggttg atccccgcgg ggtcaggatt tgaagagtat
      841 cgggatacgt tcgtcattag tgaaaaatcg gagggaacac ccgtaggtgt ttcacctgca
      901 gcacctgctg tgccatctga tgcggccgtt gtcccggcgg tttcaggaga aaccgctcgg
      961 tcttagtaat cggcggaatg ccgcctactt gacagagagc agttacgtca ctataatcct
     1021 gcggctttgc acttgacagt tagtgcttgt tccttttgaa agggtctgaa cgcgatgccg
     1081 acgatcaatc aactggtcag aaaagggcgg atctttgtaa aggcaaagac gaagagtcct
     1141 gctctcaagt cgtgtccgca gaagagaggc gtttgccttc gcgtatacac gacgacaccg
     1201 aagaagccga attcggcatt gcggaaagtt gcgcgtgtgc gtctcacaaa cggtatggag
     1261 gtgacaacct atatacccgg cgtgggacac aatcttcagg agcactcgat tgtgctagtg
     1321 cgcggaggcc gtgttaagga tcttcctggt gttcgatacc atttagttcg cggtgcttta
     1381 gatgcggtgg gtgtagccga ccgaaagcaa agtcgttcga agtatggagc aaagcggcct
     1441 aaatagattc tatggtctgg aaaatcatca cattgattgg ataggtcgat atgccacgca
     1501 gtaggttttt gggtcaacgc gaagtgcttc ctgacgtgcg gtaccgagat aaactggtcg
     1561 ggaagtttat caacacgctg atgagtaatg ggaaaaagag tacgacggaa aggatatgct
     1621 acggtgcatt cgatgttatc caggagaaga ccggcggcga cccgctgaaa gtgtttaagg
     1681 cggctgtgga taatgtgaag ccgatcgttg aagtcaagtc tcgtcgggtg ggaggtgcct
     1741 cctatcaggt tccggtcgaa attaggccgg cacgccgagt gtcgttggcg cttcgttggt
     1801 tatcgcagtt tgcccgtacg cgcggtggga aaagtatgcg ggaaaagcta gcagccgagc
     1861 tattggatgc gtcgaacaat acaggggctg cggtcaagaa acgggaagac gtgcatcgga
     1921 tggcggaggc caataaagcg ttcgctcatt atcgctggta gcgtcattct gtgctgcagt
     1981 tgagccgtgc tgcgattcgc agatccgggt gcttctccgt cgcagcagtg tgatgcggct
     2041 taatctgcag cacaagtgtt tttagagggg ttgatgtggc tcgtcagaca tcattagagc
     2101 gcacaagaaa catcggtatc atggctcaca ttgatgccgg aaaaactacg actaccgagc
     2161 ggatcctcta ctatacgggc atgacccata agatgggtga ggttcatgaa ggtgccgcca
     2221 cgatggattg gatggagcag gagcgagagc gaggcatcac cattacggct gccgccacaa
     2281 cctgtttctg gcgcgaccac cgcatcaata ttattgatac accgggccat gtggatttta
     2341 cgatcgaggt agagcgttcg ttgcgggttc tggatggggc ggtggcggcg ttcgattcag
     2401 tgcagggagt tgaacctcag tctgagacgg tttggcgtca ggcggataaa tatcacgtcc
     2461 ctcgtattgc cttcatgaat aagatggacc ggattggggc tgatttttat ggcagcgttc
     2521 aatccattat cgatcgactc ggggcgaagc cggttcctat ccagattccc atcggccgtg
     2581 aagctgaatt tcgaggatct atcgatctgg tcaggatgaa ggggtacttt tacgacgatg
     2641 agacgttggg tgcgaagtac aaggtcgatg aaattcccgg tgatctcctc gctcaagcaa
     2701 aagagtaccg cgagaagatg cttgacgcag tcgcagaatt cgatgaccag gtgatggaaa
     2761 agtatttgaa cggtcatccg ctgacggaag aagaagtgat gcgcgcgatc agagccggga
     2821 ctatttcgat gaaagttacc cccgtgcttt gcggatcggc cttcaagaac aaaggtgtgc
     2881 agcaatt
//
LOCUS       DATNDD010000244        10566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_907454, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000244 DATNDD010000000
VERSION     DATNDD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907454"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VN647_05890"
     CDS             complement(<1..957)
                     /locus_tag="VN647_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HWG96595.1"
                     /translation="MPHTDKRLTLYLIDGSAYIYRAFFALPPLSNSKGFQTNAVYGFT
                     TMLLKIMREHRPDALAVVFDEKGPTQRHEEYQAYKAQRPAMPDAMSAQVPYIHRIVEA
                     LAIPALRLAGYEADDIIGTLARKAEQADRDVAIVTSDKDMFQLLTPHVKIYDPVKDKW
                     IGEQECRERFGVEPVLVPEIMGLMGDAIDNIPGVKGIGEKTATKLIGQFRTIEELLRR
                     VEEVTPARTKTMLIEQADNARLSRRLAMIQTDCPVEFEIGALQIKPAHDDRLAELLRE
                     LEFTTLLKSIQPTSPQASIHQASVETIESEAAAQAFVARLNKS"
     gene            complement(1051..1482)
                     /locus_tag="VN647_05895"
     CDS             complement(1051..1482)
                     /locus_tag="VN647_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HWG96596.1"
                     /translation="MDWLLSAVQQYVSTETLVTLTALSVVFFVGSLIAIPFILVRLPT
                     DFFDTRVPRHWMENHHPVLRLLGHIVKNVVGAIFLFAGFLMLFLPGQGILTMLIGVTM
                     LDFPGKRRMEAKMIGQPAVLSTINNMRQKFGKPPLTIAPGQ"
     gene            1739..2158
                     /locus_tag="VN647_05900"
     CDS             1739..2158
                     /locus_tag="VN647_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96597.1"
                     /translation="MPTNPKFVIFAHNATYDKLHQVATLGMTAAAMGKDVIIVLLFWT
                     IKKLAEGKIDQIDFPPEYAESAEEVARLLKEKKVPKISEMFQDAKHVGKLRLIACSAG
                     LEYMGVDAAAVEKNVDEVLGLPAILSLTGDAETKLFI"
     gene            complement(2203..2862)
                     /gene="nth"
                     /locus_tag="VN647_05905"
     CDS             complement(2203..2862)
                     /gene="nth"
                     /locus_tag="VN647_05905"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWG96598.1"
                     /translation="MRQEQIHAAIRIVKREIRRWQEPVVGVVAKESGRDPFLILISTL
                     LSLRTKDRTTREAGDRLFAIARTPAAMLKLPLKKLEQVIYPVGFYRTKAKAIHQICRR
                     LIDEYGGVVPDSIDELVTFPGVGRKTANLVVTIGYGKPGICVDIHVHRLSNRWGYIKT
                     KTPEESEQALRRTLPKQYWIIYNDLLVPYGQNLCLPVSPLCSTCKLTELCDRVGVTRS
                     R"
     gene            complement(2926..3120)
                     /gene="rpsU"
                     /locus_tag="VN647_05910"
     CDS             complement(2926..3120)
                     /gene="rpsU"
                     /locus_tag="VN647_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWG96599.1"
                     /translation="MEIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRFYEKPSVRKK
                     AKQREAQRRRQKWLSKRRPD"
     gene            complement(3190..4656)
                     /gene="ftcD"
                     /locus_tag="VN647_05915"
     CDS             complement(3190..4656)
                     /gene="ftcD"
                     /locus_tag="VN647_05915"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWG96600.1"
                     /translation="MDRLVECVPNFSEGRDRETLHALIDAVASTAGVALLDHSMDADH
                     HRSVLTFCGSPDAIVEAAFRAVRIATELIDLRTHIGVHPRIGATDVVPFVPIRGMTMQ
                     DCVRLAKQLGERVGRELGIPVFLYEHAAGRADHVPLEAVRRGGLDGLAFRMEADPGWI
                     PDFGPPRLHQSAGAIAIGARPPLIAYNVNLCSTEVDQARSIARAVRHSNGGLPFVKAI
                     GVELSSRGIVQVATNLTDYLVTPILTAFQKIKAEATKRGIEVAGSELVGLVPQAALSQ
                     AAAASLQLERFDSSQVLETRIVEAMLSKKEPALTLSDFLAALADAKPTPAGGSAAAFV
                     GAVAASLGVMGARLGGQSDRVQRLLELSEQLYRLVQADTEVYRGLMDAYKMPKQHPDR
                     PHAISIALQRATEVPLEITELSCEVAQLLHTLRDGAKPSIHSDLTVGLTIAIAAARGG
                     LVTARTNTNVQQNHKLVDAVQLRIAKATESLEELKMLC"
     gene            complement(4744..4989)
                     /locus_tag="VN647_05920"
     CDS             complement(4744..4989)
                     /locus_tag="VN647_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWG96601.1"
                     /translation="MMQADVKLDTLGYFCPMPIILTSKKIKELALGQVLEVVSDDEGI
                     KKDMPAWCETTGHEMMGLEEEQAKSGRIYKAFVKKAK"
     gene            complement(5017..5547)
                     /locus_tag="VN647_05925"
     CDS             complement(5017..5547)
                     /locus_tag="VN647_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HWG96602.1"
                     /translation="MIATQIEPATTLAQLQESKPERVTIVLLSGDLDRAMAAFIIATG
                     AAAMGMRVTMFFTFWGLNTIRRRGATSSAKDWLRRMFGLLNKGGADTLPLSRFHFGGL
                     GTKMMQTVMKQNRMPGVPELMQTALDLGVRFIACTTTMGLMGIAKDTLIDGIDQFAGV
                     TTYLAEAKQGSVNLFI"
     gene            5737..6819
                     /locus_tag="VN647_05930"
     CDS             5737..6819
                     /locus_tag="VN647_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWG96603.1"
                     /translation="MIEQPYPEVISSKSGPLFGFWYPAVPSHTLRPGMMKGLQMLGLP
                     IVLCRDRTGGLAAMRNICPHRGMPLSFGRFDGERVECSYHGWQFDLKGRCRCIPALPE
                     QGNLQTDKIGVTTYPAEEMDGMIWLYLADERGQIHPLPPVPRMPLPSEPRQSFHISLI
                     YTCSPDDGIIGLIDPVHGPYVHSWWRSDARMHVKTKIFEPILNGFRMTAHRPAKNSGP
                     FHWMERIYGGPLTTTIDFFLPNQRVELMQCGRAWLANRLMATPMSDMECRIDFSAYWV
                     GLHWLPFGNLIFRTLTKMFLSQDERAMKHLAVGFRHKPSAMFLGDADMPAKWYYKLKA
                     AHQASVQTGRPLDHPLKERVTLRWRS"
     gene            complement(6859..8877)
                     /locus_tag="VN647_05935"
     CDS             complement(6859..8877)
                     /locus_tag="VN647_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWG96604.1"
                     /translation="MTDHSVLGNLLLIYTVSIAVVFLFHQFRLPSIAGFLVAGALIGR
                     HGFNLISDIATVQVLAEIGIVLLLFTIGIEFSLVQLASLRRLLLIAAPIQVGGVIAIA
                     WLGGTAAGLPTSQAIFWGFLLSLSSTAIVLKALAASGDSDSPHGRATIGILIFQDLAV
                     VPMILLTPILASHGDGTLTPVLFSLVKSMVVVACIVAAAWYAVPKLLDHIVRSRSREL
                     FLLTIIVMCLGIAWLTSLGGLSLALGAFIAGLVISESEYSHQAIAEVLPFRDSFNSLF
                     FVSIGILIDWRILLEHPLVVTSVLLVVLLVKFVAGAGAVLTISVPPRSAVMTGIALAQ
                     VGEFSFILAQVGLENKLLSGPPYQIFLAVSVCSMIITPFLMQLSPHLARRVEAVQRLH
                     HWFPGQTTARVLETEGRHLRIKDHVIIVGYGLNGRNLARVLGETEVPYIALDLDGDTV
                     RREAAHGLPLYYGDATNPNVLRHVKIEGARVLVVAISDPFMARRTVQVARGLNPNIRI
                     VVRTRYLRELEELHQLGVDDVVPEEFETSIEIFALVLRTYNMPQDLIVRKAEQVRREG
                     YALLRRSELPELAHHLRGGTLADVEVETCRIEEDSPAAGKTIAQLALRPRIGTSIIAL
                     TRSGVTESNPSEKTKLLAGDIVVLLGTRDQIRRAMGFILANEREDKQA"
     gene            9300..9863
                     /locus_tag="VN647_05940"
     CDS             9300..9863
                     /locus_tag="VN647_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96605.1"
                     /translation="MQSTRMFSFTVAILAVFSAAPAFSDTQTGSDVTWKCPQADGSVI
                     YTNKEKTGCQAMALKPLSIVPDLENMPTAPRTATTGEPHYEVPSYQERTYGTEGRQAP
                     DWASDWHASNTSSGSAQAEVCSLYMEWMQLVQKTRGGMFFGSDPSYGGDVTGRAQRMP
                     SYSFYDNARYIALSRIFGTGFVPIGCF"
     gene            complement(9881..>10566)
                     /locus_tag="VN647_05945"
     CDS             complement(9881..>10566)
                     /locus_tag="VN647_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWG96606.1"
                     /translation="NILEGCRLSRIEHLVYASSSSVYGGNTHMPFSTHDNVDHPVSLY
                     AATKKANELMAHCYAHLYQLPCTGLRFFTVYGPWGRPDMALFIFTKAILEGKPIEVFN
                     QGRMKRDFTYVDDIVEGVIRTLDHPATADPTWSGDRPTPETSSAPARIYNIGNNQPVE
                     LQHFIEVLELALGKKAEKKLMPLQPGDVPATYADIDDLTRDVGFKPSISIEEGIPRFV
                     KWYRKFYKV"
BASE COUNT         2346 a         2917 c         2888 g         2415 t
ORIGIN      
        1 gctcttgttc agccgtgcga caaaggcttg cgccgcggcc tcactttcga tcgtttccac
       61 ggatgcttga tggatcgaag cctgtggcga ggtgggctgg atggatttca atagtgtcgt
      121 aaattccagt tctcggagca actcggcgag ccggtcgtcg tgcgccggct tgatctgtag
      181 agcccctatc tcgaactcga ctggacaatc ggtttggatc atggccaggc gacggctcag
      241 tctggcgttg tcggcctgct cgatcaacat ggtcttggtc cgggccggcg tcacttcctc
      301 cactcgtcgg agcaattcct cgatcgtcct aaactgaccg atcagcttcg tcgccgtttt
      361 ttccccaata cccttcacac cgggaatgtt gtcgatcgca tcgcccatga gacccatgat
      421 ctccggaacc agtaccggtt caactccaaa ccgttcgcga cattcctgct cgccgatcca
      481 tttatccttg accggatcat aaatcttcac gtggggtgtg agcagctgaa acatatcctt
      541 gtcgcttgtc acgatagcga catcgcgatc ggcttgttcg gccttccgag ccagtgtgcc
      601 gataatatcg tccgcctcgt atccggccag tcggagagca ggaatcgcca gcgcttccac
      661 aatgcgatga atatagggca cctgtgcgct catggcgtcc ggcatggcgg ggcgctgggc
      721 cttgtatgct tgatattctt catgccgttg tgtagggccc ttctcatcga agaccacggc
      781 gagagcatca ggccggtgct ctcgcatgat cttgaggagc atggtggtaa acccgtaaac
      841 ggcattggtc tgaaaacctt tcgagttcga cagcggcggt aaggcaaaga aggctcgata
      901 gatataggcg ctgccgtcga ttaggtaaag ggtgaggcgt ttgtcggtgt gcggcatttc
      961 agtgtagcct tgttgagtgc tgagttctga gagccgagtc gagaccatcc gggaaatctc
     1021 agcactcagt actttcgtct cagcactgtc ttattgaccc ggcgcaattg tgagcggcgg
     1081 ctttccaaac ttctggcgca tgttgttgat ggtgctcagg acagcgggct gtccgatcat
     1141 cttggcttcc atcctccgct tgccgggaaa gtccagcatc gtcaccccga tcaacatcgt
     1201 gagaatgcct tggcccggca agaacaacat taagaaaccc gcgaacagaa aaatagctcc
     1261 gacaacattc ttgacgatat ggccaagcaa gcgaagcacg ggatgatgat tttccatcca
     1321 atgccgcggc acacgggtgt cgaagaaatc cgtcggaagc cggacgagga tgaacggaat
     1381 cgcaatcaat gatccaacga agaagacaac cgagagagcc gtcagggtca cgagggtttc
     1441 ggtcgagaca tactgctgaa ccgccgaaag aagccaatcc atcggtcggc atcctaacat
     1501 gggcttatac gcccctcaag gcaaagcagg tcctcgtctc atgtttacgc ggtcgaactt
     1561 gatcggtata atcacccggc atggactcca ggtacgaatc ggtgtaagcc gcattcgaag
     1621 gtcgtatggc tctttttcat cgcgatgtcg gcggcttcgt atctctctct tcctttcaag
     1681 gcagcgcgat caccgactgc gtcaacaacg tgaaagcccg cgcgcagaag aagtgagtat
     1741 gccgactaat ccgaagttcg tcatcttcgc tcacaacgcc acgtacgata aactgcacca
     1801 ggtcgcgacg ctcggcatga cggctgcggc gatgggcaaa gacgtgatta tcgtcttgtt
     1861 gttctggacg atcaaaaaac tcgctgaagg gaagattgat cagatcgatt tcccgccgga
     1921 atatgccgag tcggctgagg aagtcgcccg gctgctgaaa gagaagaaag tgccgaagat
     1981 ctcggagatg ttccaagatg ccaagcatgt cggaaagctg cggctgattg cctgcagtgc
     2041 cggcctagag tatatgggcg tagacgctgc tgccgtcgaa aagaacgtcg atgaggtgtt
     2101 gggcctgccg gccatccttt cgctcaccgg cgacgcagag acgaaactgt ttatctaagg
     2161 agcgtgaaac ggaaagcgtc tataccttct tgccgttcgc gtctaacggc ttcttgtcac
     2221 tcctactcga tcacacaatt cggtcaactt gcatgtgctg cacagcggcg agaccggaag
     2281 gcacaggttc tgcccatagg ggacgaggag atcgttgtag atgatccagt attgtttcgg
     2341 tagggtgcgc ctgagggcct gttcagattc ttccggtgtc ttcgttttga tatagcccca
     2401 tcgattgctg agccggtgga cgtgaatatc cacgcagatt ccaggcttgc cgtaaccaat
     2461 agtgacaacc aggttcgcgg ttttccgtcc gaccccgggg aaagtgacca gttcatcgat
     2521 ggagtccggt accactccac catattcatc gatcagcctg cggcagattt ggtgaattgc
     2581 cttggccttc gtccggtaaa agccgaccgg ataaatcacc tgttcaagct tcttgagcgg
     2641 cagcttcaac atggctgccg gtgtgcgagc tatggcgaag agccggtcgc ctgcttccct
     2701 tgttgtccta tctttggttc gtaaactgag gagagtggaa atgaggatga gaaagggatc
     2761 gcggcccgat tccttggcta cgaccccaac gaccggctct tgccaacggc gaatctcccg
     2821 cttgacgatg cggatggctg catggatctg ctcctgacgc atgggatatg gttacgaacg
     2881 tggaggaggc tggtaaggcg agcggaagag aacctgaggg tgtgtctagt caggcctccg
     2941 tttcgacaac cacttctgtc ggcgccgttg agcttcgcgt tgctttgcct tctttctcac
     3001 gctgggcttc tcgtaaaaac ggcggcgttt cagctcacga aacaacccct cacccgcgag
     3061 cttcttcttg gcgaccttca gagctttttc aacgttgttg ttaaagacct tgatttccat
     3121 ccggttgatt tccgactttc tcaggccaaa taggcctgct gcttacagct ttcaattcgg
     3181 cggcggagtc taacacaaca tcttgagttc ctcaaggctt tctgtagcct ttgcaattct
     3241 gagctgtacg gcatctacaa gtttatgatt ttgttgaaca tttgtgtttg tgcgggctgt
     3301 aacaagacct cctcgagcgg ctgcaatggc tatggtcaaa cccactgtga gatctgaatg
     3361 gatgctcggc ttagctccat cgcgcaacgt atggaggagc tgcgcaacct cgcaggacag
     3421 ctccgtgatt tctaatggca cttccgtagc ccgttggagt gcaatcgaga tcgcgtgagg
     3481 gcgatcagga tgttgtttgg gcatcttgta ggcgtccatc agccccctgt agacctccgt
     3541 atctgcttga acaagccgat aaagctgctc gcttaactca agcagacgct gcactctgtc
     3601 cgattgtcca ccgagacgtg cccccatgac ccccaaactc gccgccacag cacccacgaa
     3661 ggctgccgcg ctgccgccgg ccggtgtcgg tttggcgtcg gcaagcgcag cgaggaaatc
     3721 tgagagggtg agggccggtt ccttcttgct gagcatcgcc tcgacgattc ttgtttcgag
     3781 gacctggctt gaatcgaatc gctcgagttg cagcgaagca gcggcagcct gactcaatgc
     3841 cgcttgaggg accaagccga ccagttcgct tccggccact tctattcctc gcttcgtggc
     3901 ttcagccttg atcttctgaa atgcggtcag aatgggcgtc acgaggtagt cggtcaaatt
     3961 cgttgcaacc tgcactatac cgcgactgga tagttctaca ccgatcgctt tcacaaaggg
     4021 taacccgccg tttgagtggc ggactgctcg ggcgatggat cgggcctggt caacctccgt
     4081 cgagcagagg ttcacattgt aggcaatcag tggaggccgg gcaccgatcg cgatcgctcc
     4141 tgcgctttga tgtagacgag ggggaccgaa gtccggaatc caaccagggt ctgcctccat
     4201 ccgaaaagcc aggccgtcga gccctcctcg tctgaccgcc tctaatggaa cgtggtcggc
     4261 acgacctgca gcatgttcgt ataagaaaac ggggatcccc aattcacgcc cgacgcgttc
     4321 tcctagctgt ttggcaagcc ggacgcagtc ttgcattgtc atacccctga tcgggacaaa
     4381 cggcaccaca tccgtcgctc ctatgcgggg gtgtactcca atatgtgtac gcaggtcgat
     4441 gagttcagtt gcgatccgaa ccgcacgaaa agcggcttct actatagcgt ccgggcttcc
     4501 acagaacgtc agcaccgacc ggtggtggtc cgcatccatc gaatggtcta gaagtgcgac
     4561 gccggctgtc gaagccacgg catcaatcaa ggcgtgaaga gtttctcgat cccgaccttc
     4621 actgaaattg gggacacact cgacgagccg atccataggt ttctgcacgt attaacgaaa
     4681 aagccggagg accgatcacc gttcctccgg cttctggctt acccgtcttt ctgacaggtc
     4741 tggctatttc gcctttttaa cgaacgcttt atagatgcgg cccgactttg cttgttcctc
     4801 ttccaacccc atcatttcat ggcctgtcgt ttcacaccaa gccggcatgt cttttttgat
     4861 gccctcatca tcggagacga cttccagcac ctgtcccagc gctaattcct tgattttctt
     4921 ggatgtgagg ataatcggca ttgggcaaaa gtaccctaaa gtgtcgagtt tgacatcggc
     4981 ctgcatcatg aaaacaccct cgctccagtg aacggttcaa atgaacagat tgacactgcc
     5041 ctgcttggct tccgccagat acgtggttac gccggcgaac tgatcgatgc cgtcgatcaa
     5101 cgtatcctta gcaataccca tgagacccat cgtcgtcgta caggcgatga accgcacgcc
     5161 cagatcaagg gctgtttgca tcaattccgg cacacccggc attcgatttt gcttcatcac
     5221 cgtctgcatc atcttcgtcc ccaacccacc gaagtggaat cgagacaacg gaagcgtatc
     5281 cgccccccct ttgttgagca gcccaaacat ccgtcgaagc caatctttgg ccgaacttgt
     5341 cgctcctctt ctccgaatgg tatttaatcc ccaaaacgta aaaaacatgg tcacacgcat
     5401 acccatcgcg gcggcgcccg tggcgataat aaaggccgcc atcgcccgat cgagatcacc
     5461 gctcaacaac acgatcgtga ctctctcagg cttggattct tgcagttgtg ccaacgtcgt
     5521 cgccggttct atttgtgtgg caatcatgat ctcacctcat gagactttca gattctatgc
     5581 atgaacagaa tagtatgtga tccttcgggg tgtcaaggaa ccgggtaacc gcgcaactgt
     5641 gttcgcatag cttgacctat cgccatcctc tcgatatagt cggaattcat tgcggcatac
     5701 ccatctcgcg attttccttc ctaacgaatg accctcatga ttgaacagcc atatcccgag
     5761 gtcatatctt ccaagagcgg tccactcttt ggattctggt acccagccgt acccagccat
     5821 accctgcgcc caggcatgat gaaggggctg cagatgttgg gtttgccgat cgtcctctgc
     5881 cgtgatcgaa ccggtggact cgcggcgatg cgcaatatct gtccgcaccg gggtatgcct
     5941 ctgtcattcg gccggttcga cggtgaacga gtggaatgtt cgtatcatgg gtggcagttc
     6001 gacttgaagg ggcgctgtcg gtgcattcct gccctccccg aacaagggaa tctacaaacc
     6061 gacaagatcg gtgtcacgac ctatccggct gaagaaatgg acggcatgat ttggctgtat
     6121 ctggccgacg aacgaggtca gatccatccc ttgccgcccg ttcctcggat gccgcttcca
     6181 tcggaacctc gacaatcgtt ccacatttcc ttgatctaca cctgtagccc ggacgacggc
     6241 attatcggtc tcatcgaccc ggttcatgga ccttatgttc attcttggtg gcgcagtgat
     6301 gcgcgtatgc atgtaaagac aaagatcttt gaaccgatcc tgaacggttt ccggatgacc
     6361 gcccaccgac cggcaaagaa cagcggcccg tttcattgga tggaacgtat ctacggaggg
     6421 ccgctcacca ctacgatcga tttcttcctg ccgaatcaac gagtggaact catgcaatgc
     6481 ggaagagcat ggctcgctaa tcggttgatg gctacgccga tgtccgatat ggaatgtcgt
     6541 atcgattttt ctgcgtattg ggtagggctg cattggcttc cgttcggcaa tttgattttt
     6601 cgtacgctga cgaaaatgtt tctcagccag gatgagcggg cgatgaaaca cctggcggtg
     6661 gggtttcgcc acaaaccctc ggcgatgttt ctcggtgatg cagatatgcc ggcgaaatgg
     6721 tactacaaac tgaaggccgc ccatcaggca tcagtccaaa cgggccgacc tcttgatcat
     6781 cctctcaaag aacgagtgac gctccggtgg cgaagctgac cccgcccttt cgggtggcgc
     6841 ccatgctgat accggtggtc atgcctgctt gtcttcacgc tcattcgcca aaataaaccc
     6901 catggctcgt ctgatctgat cgcgtgttcc cagcagcacc acaatgtcgc cggcaagaag
     6961 tttggttttc tctgatggat tggattccgt gacgccgctc cgggtcaagg cgatgatgga
     7021 cgttccgatt cgcggtcgta atgccagttg agcgatggtc tttccggcag ccggcgaatc
     7081 ctcttcgatc cggcaggtct ccacttcgac atcggcgagt gtgccgccgc gaagatggtg
     7141 agccagctcg gggagctcgc tgcggcgtaa gagggcgtat ccttcacggc gaacctgttc
     7201 agcctttcgc acgatgagat cttgcggcat gttgtaggtg cggagaacga gcgcaaagat
     7261 ttcaatcgac gtttcaaatt cttccggcac gacgtcgtcc acgcccagct ggtgtaattc
     7321 ctccaattcg cgcaaatagc gtgtacgaac cacgatgcga atattcgggt tcaagcctcg
     7381 agccacctgt acggttcggc gggccatgaa cggatcggag atcgcgacga ccaagacccg
     7441 tgcgccttcg atcttcacgt gcctcaacac attcgggttt gtggcatcgc catagtagag
     7501 cggcaagccg tgggccgctt ctcggcgcac tgtatccccg tccaaatcca acgcgatgta
     7561 gggcacttct gtctcaccca acacacgggc cagattgcgt ccgttgagcc catatcctac
     7621 gataatcaca tggtccttga tgcgtagatg ccgcccctct gtttcgagca cacgggccgt
     7681 cgtctgccca ggaaaccaat ggtgcagccg ttgcacggcc tcgacgcgtc gcgcgagatg
     7741 gggggacagc tgcatcaaga acggcgtgat gatcatggaa cagaccgaga ccgccagaaa
     7801 gatttgatat ggcggtccgg acaaaagttt attctccagg ccgacctgtg ccaggataaa
     7861 actgaactcg cctacctgtg cgagggcaat tcctgtcatg acggccgagc gaggaggcac
     7921 ggagatcgtc aaaacggctc ccgctccggc gacgaatttt acgagcagta cgacgagtaa
     7981 cacactggtc acgacgagcg gatgctccag cagaatacgc caatcgatca ggatgccgat
     8041 ggagacgaaa aacaggctat tgaaactgtc tcgaaacggc aacacctcgg cgatggcttg
     8101 atgactgtac tccgattccg aaatcacgag cccggcgatg aaggctccca atgcgagcga
     8161 cagcccgccg agagacgtca accaagcaat gccgaggcac atgacaatga tggtcaacag
     8221 aaagagttct cggcttcggc tacgaacgat gtggtcgagc aatttcggaa ccgcatacca
     8281 cgcggccgcg acaatgcatg cgaccactac catggatttc accaacgaga acagcaccgg
     8341 agtgagcgtt ccatcgccat ggctggcaag aatgggcgtc aacaagatca tcgggacgac
     8401 ggccaagtcc tgaaagatca agattccgat ggtggctcgc ccgtgaggtg agtcgctgtc
     8461 tccgctggcg gccaacgctt tcaacacaat tgcggtgctg ctgagcgaga gtagaaagcc
     8521 ccaaaagatc gcttgagatg tcggtaatcc tgccgcggtg ccgccgagcc atgcaatcgc
     8581 gatgaccccc ccgacttgga tcggagcagc aatcaagagc agccgacgaa gcgaggccag
     8641 ttgcaccagt gagaattcga taccgatagt aaacagcagt agcacaatcc cgatttccgc
     8701 cagcacttgc accgtcgcga tatcagaaat cagattgaac ccatgacgac cgatcaaagc
     8761 cccggcgacg agaaacccgg caatagacgg cagtcgaaat tgatgaaaca gaaacaccac
     8821 tgcgatggag acggtataaa tgaggagcaa attgccgagt acgctgtggt cagtcatgtc
     8881 gcgtcttcaa ctcgtttcct cgtggtgatg cgtctcgtaa tggcaccctt gacaatcgac
     8941 ggcgtcttcc atcgcaatca caggtcactc ggccaacgtc aaggagatac gcaggatacc
     9001 tgaggtctcc gatagggaca aggtaaagca ggatgtcggc tgggcacttt tgtgtactga
     9061 attcagcgct ttgtaatact cgcggtcctg ggagggagcg gcttggtgat attgagaacg
     9121 atgcggtgag gatcagcgat cgcccttgtc tctaaacagg gtccagcatt gatttcccct
     9181 ctaaaggggc atagaccaaa gaataaagta ttgcgtaggg tgtagggcac agtttcatag
     9241 gaatgaccat ggagtgtcta ggcactgagt gccctcgcta cacgatccgg aggtgctcta
     9301 tgcagtcgac gaggatgttc tctttcacag ttgcaatact agcagtcttt tccgctgctc
     9361 cggcattctc agacacgcag accggatcgg atgtgacttg gaaatgtcct caagcagacg
     9421 gcagtgtaat ctacaccaac aaggagaaga cgggctgcca agctatggcg ctcaaaccgc
     9481 tttctattgt gccggatttg gagaatatgc cgacggcgcc tcgaaccgcg acgaccggcg
     9541 aacctcatta tgaggtacct tcctaccagg aacgtactta tggaacggaa ggacgtcagg
     9601 cgccggattg ggcgagtgac tggcatgcga gcaatacgtc ttccggctca gcacaggccg
     9661 aagtgtgttc gttatacatg gaatggatgc aattggtgca gaagacccgt ggagggatgt
     9721 tcttcggatc cgacccttcc tatgggggag atgtcacggg gcgtgctcaa cggatgccaa
     9781 gttactcttt ttacgataat gcgcgctata tcgcactgtc gaggatcttt ggtacggggt
     9841 ttgtgcctat tggttgtttc tagattttcg gccaggcttg tcagacctta tagaacttcc
     9901 gataccactt cacgaaacga ggaataccct cttcgatcga tatgctcggc ttgaatccga
     9961 catcgcgggt gagatcgtcg atatcggcat aagtcgcggg gacgtctccg ggttgtaacg
    10021 gcatcagttt cttctcagcc ttcttgccta atgcgagctc caggacttcg atgaagtgct
    10081 gcagttcaac cggctgattg ttgccgatat tgtagattcg tgccggagcc gagctggttt
    10141 ctggtgtcgg ccgatcccca gaccatgttg gatcggccgt cgccgggtga tcaagcgttc
    10201 ggatgacccc ctcgacgatg tcgtccacat aggtgaaatc gcgtttcatc cggccctggt
    10261 taaagacttc tatcggtttg ccctccaaga tagccttggt aaagatgaag agagccatgt
    10321 cagggcgccc ccacggtcca tagacggtaa aaaatcgaag accggtgcag ggtagttgat
    10381 agagatgcgc atagcagtga gccatcagtt cattcgcttt tttggtggcg gcatacagcg
    10441 acaccggatg atctacgttg tcatgggtgg agaacggcat atgagtgttc cccccataga
    10501 cagaactgga cgaggcgtag actaaatgct cgatgcggct cagccggcag ccttccagaa
    10561 tgttga
//
LOCUS       DATNDD010000245         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4043021, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000245 DATNDD010000000
VERSION     DATNDD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4043021"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VN647_05950"
     CDS             <1..284
                     /locus_tag="VN647_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96607.1"
                     /translation="LLTDIVMPGMSGTELARHLRVIKPELKLLFISGYTDDVGIGAGD
                     PASAYLQKPFTPEALASSVRELLDVEPTNRMRVSQQSESNEQSRSAKRS"
     gene            327..1175
                     /locus_tag="VN647_05955"
     CDS             327..1175
                     /locus_tag="VN647_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96608.1"
                     /translation="MNRSRWFEGAKHVGRVLLLSVVLLPGLSPAQDISWERLSDGLTV
                     AVWNPGEACPTVPNMLVVDMDPERTKFSVHHYAQEGLSEPPRIDEWQKRTGHHVLFNA
                     GLFRENFAYLGLLYKDGRPLGSRRHAAWQGLFVAEPSGSGLQKARILDLAAESFDEQQ
                     PRYREAAQALMLLDLTGKVRVRESGKHAYQTIVAETEAGHILLLKSLGAVRLYDIGRC
                     FKDALPAVRQAMAMDGGSSSDVRILESLWEKDLQVQEHASWKGLFGGSTGSHIPLPTV
                     IGASPR"
     gene            1175..1492
                     /locus_tag="VN647_05960"
     CDS             1175..1492
                     /locus_tag="VN647_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96609.1"
                     /translation="MVKSWLKIVVTAACLAVGGCADGAKIVQQHDSGGVVVYPFKEGQ
                     GSMLSSFRKDALDIMKEKCRGRSYSIVREGETKGRARVVSPLEGADELIEERRWGIQF
                     ECK"
     gene            complement(1526..2101)
                     /gene="pyrE"
                     /locus_tag="VN647_05965"
     CDS             complement(1526..2101)
                     /gene="pyrE"
                     /locus_tag="VN647_05965"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWG96610.1"
                     /translation="MAVRERLAQAFHDTQSFKWDRDKGFKLASGEVSPFYVDCRALMA
                     HPEARRLVAQLAYEALTDIEFDCLGGLELGAIPIALTISDFACAASRRRLWRTFVVRK
                     QPKDHGLGRLIEGSIRPGDRALIVDDVLTSGGSLLKAMGVARETGLRVDHALVIVDRQ
                     EQDGRARVEREQVQLISLLTVQDLLSTVTKD"
     gene            complement(2177..>2469)
                     /locus_tag="VN647_05970"
     CDS             complement(2177..>2469)
                     /locus_tag="VN647_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HWG96611.1"
                     /translation="DALFAVAYNEFCAPTLEESVEALIKQGATHITVTTTMFTPGGSH
                     SEVEIPEILDHLRPKHPQVVLCYAWPFDLELVANTLAEQIRRFSTAAALSDR"
BASE COUNT          544 a          651 c          725 g          549 t
ORIGIN      
        1 tgttactcac ggatatcgtg atgccgggga tgagcggcac tgaactggcg aggcatcttc
       61 gggtgatcaa gccggaattg aagcttctct tcatttcagg atataccgat gatgtcggta
      121 tcggagccgg cgaccctgcc agtgcctatc tgcagaagcc ctttacacct gaagctttgg
      181 caagttccgt gcgtgaactt ttggatgtgg agccgacaaa tcggatgaga gtcagtcaac
      241 aatcggagtc caatgagcag tcacggagcg ccaagcgatc ctgaaccatg agtcgtcctc
      301 agtacgaccg gatctgcaga gccgtcatga atagaagccg atggttcgaa ggagccaagc
      361 atgtcgggcg tgtccttcta ctgagcgtcg ttctactgcc cggcctgagt ccagctcagg
      421 atatctcgtg ggagcgcctt tctgatgggt taactgttgc agtgtggaac ccgggcgagg
      481 cttgtcctac cgtcccgaac atgttggttg tcgatatgga tccggaacga acgaagttct
      541 ccgttcatca ttatgcacag gaaggccttt cagagcctcc cagaatcgac gaatggcaga
      601 agcgaacagg tcatcatgtg ttgttcaatg cggggttgtt tcgtgaaaac ttcgcgtatc
      661 tcggtcttct ctataaggac ggccgtccgt tgggaagccg acgccacgcg gcgtggcagg
      721 gattgtttgt ggccgaaccg tccggttctg ggttgcagaa ggcgcggatc cttgatctgg
      781 cggcagagag tttcgacgaa caacagccgc gttaccgcga agcggcacag gctttgatgc
      841 ttctggacct gaccggcaag gtccgcgtcc gcgagagtgg gaaacatgcc tatcagacga
      901 tcgtcgctga aacagaggcc ggacacatcc ttcttcttaa gagtctcgga gccgttcgtt
      961 tatacgacat cggtcggtgt ttcaaggatg cgcttccagc tgtacgtcag gcaatggcaa
     1021 tggacggtgg gtcttcttcg gatgtccgca tcttggagtc gctgtgggaa aaagacctac
     1081 aggtgcaaga acacgcttcc tggaagggtc tgttcggtgg aagcaccggt tctcatattc
     1141 cgttgcccac agtgattggg gccagtccca gataatggtc aaatcttggc tgaaaattgt
     1201 cgtgacggcg gcatgcctgg cggttggagg ctgtgccgat ggcgcaaaga tcgttcagca
     1261 gcatgactcg ggtggggtcg tcgtctatcc gtttaaagaa ggacaaggct ctatgctctc
     1321 ttctttccgc aaggatgccc ttgacatcat gaaagaaaaa tgtagagggc gatcctacag
     1381 tatcgttcgc gagggagaaa ccaaaggccg cgcgcgagtt gtgagtccac ttgagggggc
     1441 ggacgaactg atagaggagc gacggtgggg aattcagttc gagtgcaaat gaatggagcg
     1501 gaacgcacaa gcaatgcagg ttgcactagt cctttgttac ggtgctcagg agatcctgaa
     1561 cggttaagag actgataagc tgaacctgtt ctctttccac ccgcgctctc ccatcctgct
     1621 cttgacggtc cacaatgact aatgcatgat cgacccgaag tccggtctct cgtgcaactc
     1681 ccatcgcttt cagcaatgac ccaccgctcg tgagcacgtc gtccacgatc agcgctcgat
     1741 cgccgggacg aatgctgccc tcgatcaacc ttcccagtcc atggtctttg ggttgtttgc
     1801 gaactacgaa tgtccgccag agacgccgac gcgaagcagc gcaggcaaaa tcggaaatgg
     1861 tcagggcgat cgggatggct ccgagttcga ggccgcccag gcaatcgaac tcaatgtcag
     1921 tgagcgcttc ataggctagt tgggcgacca gacgacgcgc ttccggatgc gccatgagcg
     1981 cacgacagtc gacatagaaa gggctgacct cgcctgatgc gagcttgaac cccttgtcgc
     2041 gatcccattt aaatgactgg gtatcatgga acgcttgcgc cagtcgctcc cgaaccgcca
     2101 cgataggttc tccctctttc agagtccgtt acgggcgagc attctacact gcctggccga
     2161 ctggtgacag agcttttcat cgatccgaga gagcagctgc cgtggagaag cgacggatct
     2221 gctcggctag cgtgttcgcg accaactcaa gatcaaaggg ccaagcataa cagaggacca
     2281 cctgaggatg tttcggtcgg agatggtcga gtatttcggg gatctcgacc tccgaatggg
     2341 atccgcctgg cgtgaacatc gtcgttgtca cggtaatatg cgtcgccccc tgcttgatca
     2401 acgcctcgac tgattcctcc aacgtcggtg cacaaaactc attgtaggcc acggcaaaga
     2461 gcgcgtcct
//
LOCUS       DATNDD010000246         4522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_643369, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000246 DATNDD010000000
VERSION     DATNDD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643369"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VN647_05975"
     CDS             complement(<1..253)
                     /locus_tag="VN647_05975"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWG96612.1"
                     /translation="MSTDRCAVRRQSRSTQGIAHQAGSGRVGSSLVLMAGNTFGRIFT
                     VTSFGESHGPAIGCVVDGCPPGLVLSVEDIQKDLDRRKPG"
     gene            complement(172..1935)
                     /gene="aspS"
                     /locus_tag="VN647_05980"
     CDS             complement(172..1935)
                     /gene="aspS"
                     /locus_tag="VN647_05980"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWG96613.1"
                     /translation="MNIRTHRCGELSKKQIGQTVVLNGWVQRRRDHGMVIFIDLRDRT
                     GITQVVFNAERNLRVHQAAHSLRSECVVSVTGQVMARPDESKNPHLSTGEVEVFVDEV
                     EILNEAKTPPFLVEDDAEVTEAIRLKYRFLDLRRPRMQRLLSLRHGIMQTTRGFLNAE
                     GFLEVETPILTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLEMSFVDREQIMSLMERMIVAIFGTVGGIQLPTPFPRM
                     TYAEAMGRYGSDKPDLRFEMPLYDMTAFGATSQFKVFKDAAAKGGIVKVLIVKGGAAL
                     SRTRIDALGETAKSFGAKGLAWLKITAEGQLESVIAKFLDVKAFATALPEAKPGDLVL
                     FGADKPAVVHDVMGRIRLLLGEELKLIDTSAWRPWWVIDFPLLDYDHEQKRYVAMHHP
                     FTAPLDEDLALFESDPLKVRAKAYDMVLNGSEIGGGSIRIHRRDAQSKVFDLLGIGKE
                     EASAKFGFLLDALEYGAPPHGGIAFGLDRLVMLLGNADSIRDVIAFPKTQKAQCPLTD
                     APSAVSLDQLKELHIKLDLVE"
     gene            complement(1932..2777)
                     /locus_tag="VN647_05985"
     CDS             complement(1932..2777)
                     /locus_tag="VN647_05985"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWG96614.1"
                     /translation="MEVRLQKLIASTGLSSRRKAEMLIASGRVSVNGKVVTELGTKVD
                     PARDHVKVDGKHLTSAQPFVYLLLNKPRNVMSTLDDPGGRTTVKDFLRGVYVRVFPVG
                     RLDFDSEGLMLLTNNGELAQALLHPRYHVPKTYLIKVKGVLRDEEIVRLERGVRLEDG
                     MTSPAHVKKVRKVEANSWLEITIREGRKHQVKRMLDAVGHAVLRLLRVRMGPLSLGNL
                     EPGEFRFLTDREANALRELVDERMALVERGNEPAPRPKRPGRGDGWAKPERRKKLSGK
                     KVKVV"
     gene            complement(2836..>4522)
                     /locus_tag="VN647_05990"
     CDS             complement(2836..>4522)
                     /locus_tag="VN647_05990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWG96615.1"
                     /translation="QSLVAVSVFASNVLFWKKSGYFDTAAELKPLLHTWSLAVEEQYY
                     VFFPIFLVLSWRLGKSKICGLLVIVFVMSLAVAQWASLAEPAAGFYLLPTRGWELLVG
                     VFSAFFVTSFPKDCIPTHIRDVGGFMGLALIVYAIFAFDKETPFPSLYTLAPTIGTAL
                     LIVCAMQGTIISKLLGNKILVGIGLVSYSSYLWHQPLLSFARHLSLGEPSKVLLGGLA
                     ALSLILGYFSWKCVELPFRNKNFVSRQTIVICGLVGTFFFLVMGLAGHVFYERYKQIW
                     LSTQTQETRATFQVIEEAQESRNYRQFVGRKRGDGECRFNVERLTTETEEEILSCSKK
                     HGKGIAILGDSHAIDLFSISLSSSQEPFLIGVTQIKCRPHSPEPQCQYEGFLSFVKKY
                     PDVFTGVIYEQAGIYFLTQGNRSGTRKIFSEVPLGESVFKVSVSERPVRAVYDYLMDL
                     SKYVKVVWVGARVEPHISPKTILNKGCAYPYDLRINQKEAFNSLDSFIENLVAGDSSI
                     RFVSQQVLLNFEFPRDFMNCEQLFWVDGDHLSLSGIKNFASRFDLIALVKYLL"
BASE COUNT         1123 a         1342 c         1042 g         1015 t
ORIGIN      
        1 taccgggctt acgccggtcg agatctttct gaatgtcttc caccgacagc acgagaccag
       61 gtggacagcc gtcgaccaca caaccgatgg ccgggccatg gctctcgccg aatgaggtga
      121 cagtaaagat tctgccgaag gtattgccgg ccatgagaac gagagagctc cctactcgac
      181 cagatccagc ttgatgtgca attccttgag ttgatcgaga ctgacggcgg acggcgcatc
      241 ggtcagtgga cattgcgcct tctgagtctt cgggaaggcg atgacgtcgc ggatcgaatc
      301 ggcgttgccc agcagcatga ccaaacgatc caatccaaac gcaattcccc cgtgtggcgg
      361 cgccccgtac tcaagcgcat cgagcaagaa tccaaactta gccgatgctt cctccttgcc
      421 gatcccgaga aggtcaaaga ctttgctttg cgcgtcgcgc cggtggatac gaatgctgcc
      481 gccgccgatt tcgctcccgt tgagcaccat gtcgtaggct ttcgcccgta ccttcagcgg
      541 atcggactcg aacaatgcca ggtcttcatc aagcggggcg gtgaatggat gatgcatggc
      601 cacataccgt ttctgctcgt gatcgtaatc caacagggga aaatcgatca cccaccatgg
      661 ccgccacgcg gaggtgtcga tcagcttcaa ctcttctccc aagagaagcc gaatccgccc
      721 catcacatcg tgaacgaccg ccggtttatc ggcaccgaat aagaccagat caccgggctt
      781 cgcttccggc agagctgtgg caaacgcttt aacatccaag aatttggcaa tcaccgattc
      841 aagctgccct tccgccgtaa ttttgagcca tgccagaccc ttggcaccga agctcttcgc
      901 cgtttcgccc aacgcgtcga tgcgcgtccg agacagcgcc gcccctcctt ttacgattaa
      961 gaccttgacg ataccgcctt tggccgccgc atccttgaag actttaaact ggctcgtcgc
     1021 tccaaacgcc gtcatatcgt agagcggcat ctcgaaccgg agatcaggct tatccgaccc
     1081 atatcgcccc atcgcctcgg catatgtcat acgcgggaac ggcgtcggca actgaatacc
     1141 cccgaccgtc ccgaagatgg cgacgatcat gcgttccatg aggctcatga tctgctcgcg
     1201 atcaacgaac gacatttcca ggtcgatttg ggtgaattcg ggctgccgat cgttgcggag
     1261 gtcttcgtca cggaaacagc gggcgatctg atagtagcga tcaactccgc tgaccatcag
     1321 gacttgcttg aacaattgcg gcgactgcgg cagtgcgaag aactgtccgg cattgacgcg
     1381 gctcggcacc agataatccc gagccccttc cggtgtgctc ttcgtcaaaa tcggggtctc
     1441 aacttccaga aacccttccg cattcagaaa acctcttgtc gtctgcatga tgccgtggcg
     1501 caagctcaag agccgctgca tcctggggcg acgcagatcc agaaagcggt atttcaaccg
     1561 gatcgcttcc gtcacctccg catcgtcttc gaccaggaac ggcggcgtct tggcctcatt
     1621 cagaatttcc acctcgtcga cgaagacctc gacttccccg gtggagagat gggggttctt
     1681 cgattcatcc ggccgcgcca tgacttgacc ggtgaccgac acgacacact cgctgcggag
     1741 cgaatgggcg gcctgatgta ccctgagatt tcgttctgcg ttgaatacga cctgtgtgat
     1801 gcccgttcga tcacgaagat cgatgaagat gaccatgcca tgatcgcgcc gacgctgaac
     1861 ccatccattt aagacgacgg tctgaccgat ctgtttcttg ctcaactctc cacaacggtg
     1921 agtgcggatg ttcatacaac cttcactttc ttccctgaaa gcttttttct tctctcgggc
     1981 ttggcccaac catctcccct gcccggccgc ttcggtctcg gtgccggctc atttccccgt
     2041 tcaaccaacg ccatccgctc atcgaccaac tcgcgtagcg cgttggcttc tcgatccgtc
     2101 agaaaccgaa attcccctgg ttccagattg ccgagcgaga gaggccccat cctgacccga
     2161 agtaatctga gcaccgcatg tccgacggca tccaacatgc gcttgacttg atgtttgcgt
     2221 ccctcacgga tggtgatctc cagccaggag ttcgcctcga cctttctcac cttcttcacg
     2281 tgggccggac tcgtcatccc atcttcgagt ctcacccccc gctccagacg aacgatctcc
     2341 tcatccctca acacaccctt caccttaatc agataggtct tcggcacatg atagcgcgga
     2401 tgcaacaggg cctgcgcgag ttcgccgttg tttgtcagca acatcaaccc ttcgctgtcg
     2461 aaatcgagcc gaccgacagg gaacacgcga acgtatacac cccgcaagaa atccttcacg
     2521 gtcgtccgtc cacctggatc atcgagcgtt gacatcacgt ttcttggttt attcagcagc
     2581 agatatacaa atggctgcgc cgacgtgagg tgcttcccat cgaccttcac atgatcgcgg
     2641 gctggatcga ccttcgtccc gagttccgtg acgaccttgc cgttcaccga cactcgtccg
     2701 gaggcgatca acatctcagc ttttcgacgg gatgatagcc ccgtactcgc aatgagtttt
     2761 tggagtctga cttccatcgg cttttcactc aatcatcatg cagaactttc aggcgtctta
     2821 tcacaatcct aacgttcaca gcaaatactt cacaagcgca atcaaatcga atcgactggc
     2881 aaaattcttt atgccgctca aactcaggtg atcaccatct acccaaaata attgctcgca
     2941 gttcataaag tccctcggaa attcaaagtt taaaagaact tgttgtgaaa caaaccttat
     3001 cgaagaatcc cctgccacca aattctcaat aaaactatcc aagctattga acgcttcttt
     3061 ctggttgata cgaagatcgt acggatacgc gcatccttta ttcaaaattg ttttggggct
     3121 aatatgcggc tcaacgcgag cccctaccca cacaaccttc acgtacttac taaggtccat
     3181 taggtagtca tataccgcgc gaacaggcct ttcactaacc gagaccttga acacagattc
     3241 acctaacgga acctcagaaa atattttacg cgtgccgctt ctgttcccct gtgtcaaaaa
     3301 atagattccc gcttgctcgt aaatgacacc ggtaaagaca tcgggatatt ttttgacaaa
     3361 ggacaagaac ccttcgtact gacactgagg ctccggcgaa tgagggcggc atttaatttg
     3421 cgtaacacca atcaaaaatg gctcttgaga actagaaagc gagatagaaa aaagatcgat
     3481 tgcgtgactg tcacctaaaa tcgcaatacc cttaccgtgc ttcttagagc aagaaagaat
     3541 ttcttcttca gtttcagtcg tcagtcgctc gacattgaaa cggcactctc cgtcaccacg
     3601 cttccttcca acgaactgcc ggtaattacg gctctcctga gcttcttcga tcacctgaaa
     3661 cgtcgctcgc gtttcctgtg tttgtgtgga tagccaaatt tgcttatatc gttcgtagaa
     3721 gacatgtcct gctaaaccca taacaagaaa gaaaaacgta cccacgagac cacagatgac
     3781 gatagtctgt cgactgacaa agtttttatt tctgaacgga agctcgacgc acttccaact
     3841 aaagtagcca agaatcagcg agagtgcagc aagtccccca agtagtacct tactgggctc
     3901 ccccaagcta aggtgtcgcg caaaagacaa taatggctga tgccataagt aggagctgta
     3961 gctgaccaaa cctatgccaa ccaaaatctt gttgccgagc agcttgctaa taatagtgcc
     4021 ctgcatagca cacacgatga gcagcgcggt gccaatagta ggcgccagcg tgtagagact
     4081 tggaaatggc gtttccttat cgaaagcaaa tattgcgtat acgatgagcg caaggcccat
     4141 aaagccgcca acgtccctga tgtgcgttgg aatacagtct ttggggaagc tcgtgacaaa
     4201 aaaagcgctg aaaacgccca cgagcaattc ccagccacgt gtaggaagca gataaaaacc
     4261 ggccgccggc tcagcgagtg aagcccattg cgccacagcc agactcatca caaatacaat
     4321 gacaagcaaa ccacaaattt tgctcttacc taacctccaa ctcaagacca ggaaaatcgg
     4381 aaagaacacg taatattgct cttcaacagc caaactccac gtgtgtaaca atggcttaag
     4441 ttcagcagcc gtatcaaagt acccgctttt tttccaaaac agcacattcg aagcaaacac
     4501 cgaaaccgcg accaagcttt gc
//
LOCUS       DATNDD010000247         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_145576, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000247 DATNDD010000000
VERSION     DATNDD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145576"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VN647_05995"
     CDS             complement(<1..1190)
                     /locus_tag="VN647_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWG96616.1"
                     /translation="MCLVVVLSWVCVARPGDAADISPARFATPNGMTVLVLEQHFLPI
                     VEIHALIKAGSAQDPPEKAGLANLVAGLLDEGTTTRSSKQLAEQIDFVGGSLGAQADE
                     DFTTASARTLKKDIDLGFTLLADILQRPAFPKQEFERVRSQILGEIASDNDDPGHVAM
                     KAFNQLVFQNHPYRWPVNGTEDTLGKITLADVQAFYAKEYLPNQVILTVVGDVSVEQA
                     TTLVQTHFGSWKKGAVQPRTAKKPAVIDKKVVQLIEKDLTQSTIVIGHPGISRTNPDF
                     YAVTVMNHVLGAGGFSSRLMDTIRDKQGLAYGITSHYDARLMPGSFWINLQTRTETTN
                     QAINGVLTEMKTIREAPVSDQELADAKAFLMGSFPLRLDSTAKLAKVLAQVEFFGLGF
                     DYFS"
     gene            complement(1264..>2263)
                     /locus_tag="VN647_06000"
     CDS             complement(1264..>2263)
                     /locus_tag="VN647_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWG96617.1"
                     /translation="ALELEADRMQGLILDHNEFQTERDVVKEERRLRSEDDPQGALVE
                     ALFAQAFLSHPYHWPVIGWFADLDAMSLEDLQRHYDTFYSPNNATLVVVGDIKAESLL
                     PTIKRLFEPIPRGPSPKQTLSPEPEQRGERRFLLKREAQVPFVMMGFRVPNYSNEDSY
                     ALDILESILSHGKSSRLYQSLVYDQKNSLAVGAEYSVLQTDPGLFYFYSLVNPSAKVE
                     GVEEAIQREIVRFQNEPPSEQELQRAKNQVEAARIFEQDSNFRHAMLMGQAESVGAGW
                     RRIDQFVERIRAVTAKDIQRVAKQYLTSDNRTVGILIPLPSPPPDLVPTAVHDGKS"
BASE COUNT          485 a          611 c          657 g          510 t
ORIGIN      
        1 tgactgaaat agtcgaatcc taacccgaaa aactccactt gtgccaagac tttcgccagc
       61 ttcgctgtag aatccagccg caaggggaag ctgcccatca aaaatgcttt agcatcggcc
      121 aattcctgat cgctgaccgg ggcttctcga atcgtcttca tttcagtcaa gacgccgttg
      181 atggcctgat tagtggtttc agtgcgagtc tggaggttga tccagaacga acccggcatc
      241 aatcgggcgt cgtaatgact ggtgatgcca taggcaaggc cctgcttgtc tcgaatggta
      301 tccatgagtc gggatgaaaa tcctccggcc cccaacacat gattcatgac ggtgacagcg
      361 taaaaatccg ggttggtccg actgattccg ggatggccta tcacaatggt cgattgggtg
      421 aggtcctttt caatgagctg cacgaccttt ttgtcgatga cagcgggctt cttggccgtt
      481 ctgggttgca ccgccccttt cttccaggat ccgaaatggg tctggacgag cgtcgtcgct
      541 tgttccaccg agacgtcacc gacgacggtg aggatgactt gattgggaag atattctttg
      601 gcgtagaagg cctgcacatc cgccaaggtg atcttgccca acgtgtcctc cgtcccgttc
      661 accggccaac gatagggatg attctgaaag accagctgat tgaaggcttt catggcgaca
      721 tgcccaggat cgtcattatc gctggcaatt tccccaagga tctgcgatcg gactcgttcg
      781 aactcttgtt tgggaaaagc ggggcgctga agaatatcgg cgaggagagt gaacccgagg
      841 tcgatgtctt tcttcagcgt gcgcgccgac gcggtcgtga aatcttcgtc cgcctgagct
      901 cccaacgaac caccgacaaa gtcgatctgt tcggccagct gtttggagga tctggttgtg
      961 gtgccttcat cgaggagacc ggcgactaaa ttcgccagac cggccttttc tggaggatcc
     1021 tgcgctgagc cggccttgat gagggcatgg atttcaacga tggggaggaa atgttgttcc
     1081 aacacgagaa cggtcatccc attcggagtg gcgaatctcg cgggtgaaat gtcagcggca
     1141 tcacccggcc tcgcgacgca cacccagctc aggaccacca ccaggcacat gaccgaccac
     1201 cgtcgaacgg atctaggggc aggggaatgg cgcttctgct ctctcggctt gatgaccatg
     1261 attctatgac ttcccgtcat gcacggcagt gggtaccaag tctggggggg gagagggcaa
     1321 aggaatgaga attccgacgg ttcgattgtc cgaggtgaga tattgttttg caacacgctg
     1381 gatgtccttt gccgtaaccg cgcggatacg ttccacgaat tgatcgatcc gccgccagcc
     1441 ggcgccgacg gattccgcct gccccatcaa catggcatgg cgaaaattcg aatcttgctc
     1501 aaagatgcgc gccgcctcaa cttggttctt ggcccgctga agctcctgct cggacggcgg
     1561 ctcattctgg aaccgcacga tctcacgttg aatggcttct tcgacccctt caaccttcgc
     1621 gctgggattc accaatgagt agaaataaaa caggccggga tctgtttgca gcacactgta
     1681 ttcggcgccg acagccagcg aattcttctg gtcatagacc aagctctggt agagacggga
     1741 gcttttcccg tgagacagga tcgattcgag gatgtcgaga gcatacgagt cctcgttcga
     1801 atagttgggc acgcggaacc ccatcatgac gaacggtact tgcgcttcgc gcttcaggag
     1861 aaaacggcgc tcgccgcgct gttccggttc cggcgacagg gtttgcttgg gtgaggggcc
     1921 tctggggatc ggctcaaaca gacgtttgat cgtcggaagt agagattcgg ccttgatatc
     1981 gcccacaacc actagggtcg cattgttggg agagtaaaag gtgtcgtagt ggcgttgcaa
     2041 gtcttcgaga gacatcgcat cgagatcggc gaaccatccg atgacgggcc aatggtaggg
     2101 atggctgagg aaggcctggg cgaacagcgc ttcaaccagc gcaccctgcg ggtcatcctc
     2161 ggatctgaga cggcgttctt ctttcacgac gtcgcgctcg gtctgaaatt cgttgtgatc
     2221 gagaatcagg ccctgcatcc gatcggcctc cagttcgaga gcc
//
LOCUS       DATNDD010000248         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4039895, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000248 DATNDD010000000
VERSION     DATNDD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039895"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VN647_06005"
     CDS             <1..632
                     /locus_tag="VN647_06005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWG96618.1"
                     /translation="NRDKIKREWCIGYGPILLTVARLTEEKGHRFLIESLPGLLAEWP
                     SLICLFVGEGECRESLRALAREKGVEQSCRFSGARNDVADWYAAADVVVLPSLSEGFP
                     FVVLEALAMSRPVVATNVNGVPEIIHDGSTGLLVPPRNPQALEAAIRTLLHDPSLAAR
                     LGKAGQEEVAVRFTSEKMVQDTVKVIEEAMPALRCSPGVAQGHVQKEAA"
     gene            629..1516
                     /locus_tag="VN647_06010"
     CDS             629..1516
                     /locus_tag="VN647_06010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96619.1"
                     /translation="MTTEMANQETVIGCPCGAVVQLAEVFHTPTRRYVRCPACDLIFL
                     YPRPSRELVEDYFREAYDGDYGAVEACDDRQPVYRSVLKHLSLYRSSPGALLDVGCGD
                     GEFLLLCRRAGWDCTGTELSEQAATRAARKGLTIVPPHTLEDGEDGRHFDVVVLINVL
                     ETVADPLTMLRQAADLLTPRGLVMVRATNGLFHLPMRTPARLIGSRYDQAFHWYLYTT
                     RSLKTLMESVGLKVISLRNSRPSRGPLSPVHPWFSRLKWAVSRALLWPLAQTLYHTTG
                     GRIICAPSFEIVAQRPGNG"
     gene            1513..1767
                     /locus_tag="VN647_06015"
     CDS             1513..1767
                     /locus_tag="VN647_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96620.1"
                     /translation="MMSQDLTYRRSWKRTVRAWGTILFLCLLGAVAALGVRALLESVG
                     SAKHTRYEPVDVPPQSVSPRHEREREELERRDRIVLEKEK"
     gene            1772..>2145
                     /locus_tag="VN647_06020"
     CDS             1772..>2145
                     /locus_tag="VN647_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HWG96621.1"
                     /translation="MCGICGVIGEDDGSALQPMLRRLIHRGPDEEGIHRQPGVALGAR
                     RLRVIDPVGGQQPVRNERGTVWAVMNGEIYNYRELRQELMSRGHRLATRCDTEVLVHL
                     YEDEGFDAFRRLRGMFAVALWD"
BASE COUNT          438 a          546 c          677 g          484 t
ORIGIN      
        1 ctaatcgaga caagatcaaa agagagtggt gtataggtta cggtcctatc ctcctcacgg
       61 tcgccagact caccgaggaa aagggccatc ggtttttgat cgaatctcta cccgggcttc
      121 ttgcggagtg gccgtcactc atctgcttgt tcgtcgggga aggtgaatgc cgagaatcgc
      181 ttcgggcctt ggcaagagaa aagggcgtcg aacaatcttg ccgtttttcc ggagcgcgga
      241 atgacgtggc tgactggtat gcggcggcag atgtggtcgt gttgccttcg ctttctgaag
      301 gatttccttt tgtagtcctc gaggccctgg ccatgtcccg ccctgtcgtc gcaaccaatg
      361 tcaacggtgt tccagagatc attcacgacg gcagcaccgg tttgctggtg cctccaagga
      421 atccccaagc attggaagca gcgattcgga ctttgcttca tgatccgtct ctggctgcac
      481 gtttaggaaa ggcggggcaa gaagaagtcg cagtgcgttt tacgtcagag aagatggttc
      541 aagacacggt caaggtaatc gaagaagcga tgccggcttt gcgttgttca ccaggcgttg
      601 ctcagggaca tgtccagaaa gaggcggcgt gaccacggaa atggcgaacc aagaaaccgt
      661 tatcgggtgt ccctgcggag ccgttgtaca actcgctgaa gtatttcata ccccgacgcg
      721 acgatacgtt cgctgtcctg cgtgcgatct gattttcctt taccctcgtc cgagtcgcga
      781 attggtggaa gactattttc gtgaagccta cgacggcgac tacggagccg ttgaggcgtg
      841 tgatgatcga cagccggtgt accggagtgt gttgaagcac ttgtccttgt accggtcgtc
      901 gccgggggcg ttgcttgatg tcggctgtgg agacggagaa ttcttactgc tgtgtcgacg
      961 agccggatgg gactgtacgg gaaccgagtt atcagaacag gccgcgactc gtgctgctcg
     1021 aaagggattg acgatagtgc cgccgcatac gcttgaggac ggtgaagacg gtcggcattt
     1081 tgatgttgtg gtcttgatca atgtgctgga aaccgtggct gatccgttga ccatgcttcg
     1141 gcaggcggca gatctcctca cgcccagagg gttggtgatg gtacgagcca caaacggact
     1201 gttccatctg ccgatgagga cgcccgctcg cttgatcggt tcgcgatatg accaagcgtt
     1261 tcactggtat ctctatacga ccaggtcctt gaagacgctc atggaaagtg tcggtctcaa
     1321 ggtcatcagt ctgcgcaatt ccagaccgag tcgaggaccg ctttcgcccg tgcatccctg
     1381 gttcagccga ctaaaatggg cggtaagccg cgcactgctg tggccgcttg cccagactct
     1441 gtaccacaca acgggcggcc gcatcatatg cgctccgtcg tttgaaatcg tcgctcaacg
     1501 accaggaaat ggatgatgag tcaagatctc acataccgtc ggtcgtggaa acgcaccgtg
     1561 cgcgcgtggg ggacgattct ctttctttgt ctcttgggag cggtggcggc gctcggcgtg
     1621 cgggcactac tcgagtcggt cggaagcgcc aagcatacgc ggtacgagcc ggtggacgtt
     1681 ccacctcagt cggtttcgcc ccgtcatgaa cgggagcgag aagaattgga acgccgtgac
     1741 cgtatcgtgc ttgaaaagga gaagtagtgc catgtgcggc atttgtggtg tcataggaga
     1801 ggatgacggt tcggcactcc aacccatgct gcggcgtctg atccatcgtg gtccggacga
     1861 agagggaatc catcgtcagc cgggagtcgc gctcggcgct cggaggcttc gggtgattga
     1921 tccggtcggg ggacagcaac cggtccgcaa tgagaggggg acggtttggg ccgtcatgaa
     1981 cggagagatt tataactatc gagaattgcg gcaagagctc atgagcagag ggcaccgcct
     2041 cgcgactcgc tgtgataccg aagtcctggt ccacctgtat gaggacgagg gattcgatgc
     2101 ctttcgtcga ttgcgaggaa tgttcgcggt ggcgctgtgg gatca
//
LOCUS       DATNDD010000249         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_419516, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000249 DATNDD010000000
VERSION     DATNDD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419516"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VN647_06025"
     CDS             <1..868
                     /locus_tag="VN647_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96622.1"
                     /translation="SEASTKPLDPSQFLVEVKSRYIWLTTVVVALVAGLYALISCGAI
                     IYQAHPRPRLVVVIALGIFLASLGIAFIWALDETYALYRAVFSFSYDNLRQAGRQRIS
                     EDLLRYAMVVVSIINVQAMVVPVVALLAACSTLASPVSGVQPDPAFWSLQMRRLKEVL
                     SAASAILVAGVLHMGAWLRWPAALVADTKAHEALLGASLAITLFWGVTFTLMLVSTYL
                     PAALLLARRAQALLLESPPQPAAPDPEQWLKDHGLFLSLQDHLPQFGLMLAPLLASPL
                     SSLLLAPLTPTG"
     gene            complement(911..1249)
                     /locus_tag="VN647_06030"
     CDS             complement(911..1249)
                     /locus_tag="VN647_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWG96623.1"
                     /translation="MADSTAKKIDSYYSPSGKIGQKYLVSGKSISMRLWEDLQPGAQK
                     EPSVREYETVGYAIKGRAQLDIEGQLLLLTAGDSWVVPKGSRHSYTILEPFTAVEATT
                     PPAEVHGRDE"
     gene            complement(1919..>2308)
                     /locus_tag="VN647_06035"
     CDS             complement(1919..>2308)
                     /locus_tag="VN647_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96624.1"
                     /translation="GRSLNTNVITLMDLLATHPSAGPKLQAWEQDRLVLKTIKALNET
                     FESPYHRRFDFPLTFNMPSEEREPLGVLLRSLKDNGIPDTVAYLTEEELTRAQKDLED
                     IGYNSEAIRMALNAPPGPIEYCHFTCR"
BASE COUNT          473 a          653 c          618 g          564 t
ORIGIN      
        1 ctccgaagcc tcaaccaaac cgttggaccc ttcgcaattt ctggttgagg tcaagtcccg
       61 gtatatctgg ctgacgaccg tggtggttgc actggtggcc gggctttatg ctttgatctc
      121 ctgcggagcg atcatctatc aggctcatcc acgacctcgc ctggtagtcg tcatcgccct
      181 tggaatattc ctcgcctcgc ttgggattgc atttatttgg gcgctcgatg agacctatgc
      241 actctaccga gcggtcttca gctttagtta cgataaccta cgtcaggccg gacgccagcg
      301 catcagcgag gacctcttac gctatgccat ggtggtcgtg tccatcatca atgtgcaggc
      361 catggtcgtg ccggtcgtcg ccctgctcgc ggcgtgcagc acacttgcct caccagtctc
      421 gggcgttcag cccgacccgg cgttttggtc attgcagatg agacggttga aagaagtctt
      481 gagcgctgcc tcggccattc tcgttgccgg ggtcttgcat atgggggcct ggctgcgctg
      541 gccggcagcg ctggttgccg acaccaaggc gcacgaagca ctactgggcg cctccttagc
      601 catcacgctt ttttggggtg tcacatttac cttgatgttg gtatccacat atttgcccgc
      661 cgcccttctt cttgccagac gcgcacaagc attgttattg gaaagtcccc ctcaaccagc
      721 cgctccggat cctgagcaat ggctcaagga ccatggtttg ttcctctcgt tgcaagacca
      781 tttgccgcag ttcggcctca tgcttgctcc cctgcttgcc agtcccttga gctccctgct
      841 gctcgcgccg ctgactccga caggctaacg accgaggtgc tctgttgaag ggagcgaggg
      901 cgactcgacg ttattcgtct cttccgtgga cctcagccgg aggagtagtc gcctctaccg
      961 ctgtaaaggg ttccaaaatc gtgtaggagt gtcggcttcc cttgggcacc acccacgaat
     1021 cgccggcagt gagaaggagc aactgcccct cgatgtccag ttgcgcacgc cccttgatgg
     1081 catacccgac ggtttcatac tcacgtacac tgggctcctt ttgtgctccc ggttgaagat
     1141 cttcccataa gcgcatcgaa atcgatttcc cggagaccag gtatttttgc ccgattttcc
     1201 cgctgggaga atagtaagaa tcaatctttt ttgccgtgct atctgccatg agattcctcc
     1261 tctgggatca gcgccgagtg tggcagttgg aatgctttca tatccttcac gacgaacgtc
     1321 acttcgcgat cttccttcaa acatataacg ataccccttc gacctttcac tgtttcctcc
     1381 tgcattaaca ctcaccattc catcacaatt atctggttag accaccggca acctccctgg
     1441 tttgtgatga tcaaggagag actcaaaggc caggcaaaac acgatccata gtgacgatct
     1501 tgagtgaagc gtaccgacca tcgcttggcc ggacgattgt gacgaatcac aaatggccaa
     1561 gggctcttcc gagtgatgta ggagcgacta aaatcgtcat cactggagca tcacgtacgg
     1621 tggaagcggg ttaacttatg agcagtatgg acccgcctat cgctatggga caggactatc
     1681 cggcagcgac tgggcgacac tggaaccgga agcgcgccgg acatgggaag aaaagagtcc
     1741 gggaacctgg gagaagctga aagatgcggt gcgttactcg tggaatcgtg gaagggccaa
     1801 atccaaacgt caagcggcgt agcatgatct ctggaccatg aattcacatt tcttgcatcg
     1861 ccgccctcat tgtctccttc aacaaaagga gggcgggcag ccgactagga ttgcgttatc
     1921 accggcaggt gaagtgacag tattcgatgg gaccgggcgg ggcgttcaat gccattctta
     1981 tagcctcgct attgtaaccg atgtcttcca gatctttttg agcgcgggtg agttcttctt
     2041 cagtcaggta ggccacggtg tccggaatgc cgttgtcttt caatgagcgc aggagtacgc
     2101 ccaaaggctc gcgttcctca gaaggcatat tgaaggtcaa cggaaaatcg aaccgacggt
     2161 gataggggct ttcgaatgtc tcgtttaacg ctttaatggt cttcaacaca agcctgtctt
     2221 gttcccaggc ttgcagtttc ggcccggcgg acgggtgcgt cgccaacaga tccatgagcg
     2281 tgatcacatt ggtattcaac gaccgtcc
//
LOCUS       DATNDD010000250         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_315265, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000250 DATNDD010000000
VERSION     DATNDD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315265"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1027)
                     /gene="flgA"
                     /locus_tag="VN647_06040"
     CDS             complement(227..1027)
                     /gene="flgA"
                     /locus_tag="VN647_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HWG96625.1"
                     /translation="MSGFLLIVIMLLLPGVFGVTTAMAGPSGESHLISPAPRSPIHRA
                     TDTVVDRVAVSEVTPESIRKAIQKHLESEWGGKIKAVHVRVLDPADPVMISGGIVELQ
                     VINNPAGESLGRRMFHLEAAVHGKPQKTIQAVADIAAMIDAVVATRFLKTDELIEVGD
                     LKTVEMRVYQMKHPFITDQGEVIGKSAARPLPPDIPLRPTFVKLPVVIKKGDRVLIEA
                     RRGGLSIRAYGVTKSNGQLGQTVMVANLDSGRELRAKVVAPSLVEVEF"
     gene            complement(1037..1828)
                     /gene="flgG"
                     /locus_tag="VN647_06045"
     CDS             complement(1037..1828)
                     /gene="flgG"
                     /locus_tag="VN647_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HWG96626.1"
                     /translation="MIRAMWTAATGMTAQQINVDTIANNLANVNTNSFKRSRAEFADL
                     LYQIQRLPGTSASTIGVFPVGVQVGAGVRPTTVAKEWLQGNMRQTNNDLDLAVDGVGF
                     FQVTRPDGTIMYTRNGAFKRDNLGNVVTGDGDLLNPVITIPSGALKVDIGMDGTVSVL
                     LPGVTQASQVGQIQLTRFDNPSGLVAMGNNLFIDSFASGPPTQGTGGFTTGFGMIQQG
                     FLESSNVNLAEEMVSMIIAQRSYEVNSKTIQASDEMMSIANNLRR"
     gene            complement(1944..>2430)
                     /locus_tag="VN647_06050"
     CDS             complement(1944..>2430)
                     /locus_tag="VN647_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HWG96627.1"
                     /translation="GNPLDIAIQGPGFFEVNTPQGIRYSRNGMFSLDRERRLVNGLGY
                     PVMGTKGEIKISPGKLEITTQGDIKVDDKSVATIKVMEFHKDEMPQKSSDGLFSSDKG
                     KLAKTPQLQVGHIEESNVNSIGEMVKMIQGMRNYESTQKLIQTLDHMADVAIQDVGRV
                     L"
BASE COUNT          550 a          699 c          589 g          592 t
ORIGIN      
        1 catagagata agcccgcccg ttctcttcct gccacagtga accgatcgtc ttcggcggtg
       61 gcaagggagc caccactacc ttgctcgata catttggcgg actcgaacac ccttcaagaa
      121 aaaccacagc caccatccat agacaatgtg cgattattaa atgcaggcta ctattgcgca
      181 cggccttaac ctcaaccttc gggatccaca acatttcagc aaggcgctaa aattccactt
      241 caacgagact gggagcaacc actttggccc tcagctctct gcctgaatct agattagcga
      301 ccatgacggt ctgcccgagc tgtccattcg atttcgtcac cccataggcc cgaatcgaca
      361 gtcccccacg cctcgcttcg atgaggactc gatcaccctt tttgatcacc accggaagct
      421 tgacgaatgt gggacgcagt ggaatatcag gcggaagagg ccgcgcggcg ctctttccta
      481 tgacctcgcc ttgatctgta atgaagggat gtttcatctg ataaacccgc atttccacgg
      541 tcttgagatc tcctacctca atcagctcgt cagtcttaag aaaccgagtg gcgacgactg
      601 cgtcgatcat cgccgcaatg tcggcgactg cctggatcgt cttctgaggt ttcccatgca
      661 ccgccgcctc caggtgaaac atacgccgcc ctaggctctc tccagcaggg ttgttaataa
      721 cttgtagctc cactatccca ccggagatca ttaccggatc agcagggtcc aggaccctca
      781 catgcaccgc cttaatctta cctccccact cgctctccag atgtttctga atggccttcc
      841 taatcgattc gggggtgacc tcgctgacgg ctacccggtc caccacggtg tcggtcgccc
      901 gatgaatcgg acttctgggc gccggcgaga tcaagtgaga ctcaccacta ggtccagcca
      961 ttgccgtcgt cacgccaaag actcctggta aaaggagcat gatcacgatc agtaagaatc
     1021 cggacatgag atgcccctat cgtctgagat tattggcaat ggacatcatt tcgtcggagg
     1081 cctgaattgt cttggaatta acttcgtaac ttcgctgggc gatgatcatg ctgaccattt
     1141 cttcagcgag attgacattc gagctctcca aaaagccttg ctggatcata ccgaatccag
     1201 tggtgaagcc gcctgttccc tgcgtcggag gtcctgatgc aaaactgtcg ataaagaggt
     1261 tgttccccat ggcaaccaag ccggacggat tgtcaaaccg tgtcagttgg atttgtccca
     1321 cttgcgaagc ctgtgtgaca ccaggaagca atacggagac ggtgccgtcc ataccgatat
     1381 ccaccttcag tgcaccggag ggtatcgtaa tcacgggatt caggagatcg ccatcgccgg
     1441 tcacgacatt gcccaaattg tcgcgcttaa atgccccgtt tcttgtatac atgatcgtcc
     1501 cgtcgggacg agttacttga aagaacccga ctccatcgac ggccaggtcc aagtcgttgt
     1561 tcgtttgccg catattgcct tgcagccatt cttttgccac tgtcgttgga cgcaccccgg
     1621 cgcctacctg aacgccgacg ggaaagactc cgatggtgga tgcgctcgtg ccgggaagtc
     1681 gttgaatttg atagagcaag tcagcaaact cagcgcggct gcgtttgaaa gagttggtat
     1741 tgacgttggc aaggttattg gcgatggtgt cgacattaat ctgctgagcc gtcattccag
     1801 tcgccgctgt ccacatggca cggatcatag aatcctccag agctgaagaa ccgatcgcag
     1861 atagctgaga acaagagatt tcgcatcaga tgatatccag gccgtgccac cacttagtgg
     1921 ccgtctgcga tacacattcc ttctcatagc acccgaccta cgtcttgaat tgcgacatca
     1981 gccatatgat caagcgtctg aatcagcttt tgcgtcgatt catagttgcg catgccctga
     2041 atcattttca ccatctcgcc gatggaattg acgttggact cttcgatgtg tccgacctga
     2101 agctgtgggg ttttggcgag tttgcctttg tcggaggaaa ataagccatc cgatgatttt
     2161 tgcggcatct cgtctttgtg gaactccatg actttgatcg tcgccacaga tttatcatcg
     2221 actttgatgt ctccctgtgt cgtaatctcc aatttaccgg gagagatctt gatctcaccc
     2281 ttcgtcccca tcaccggata gccgagtccg ttcactaacc gacgctcacg atcgagcgaa
     2341 aacatgccgt tgcggctgta tcgaattcct tgtggagtat tcacctcgaa aaaccctggc
     2401 ccctgaatgg cgatgtccaa cggattcccg
//
LOCUS       DATNDD010000251         4818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2392054, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000251 DATNDD010000000
VERSION     DATNDD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392054"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="hisG"
                     /locus_tag="VN647_06055"
     CDS             complement(<1..500)
                     /gene="hisG"
                     /locus_tag="VN647_06055"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWG96628.1"
                     /translation="MLTIALSKGKLIESALDLFRRAGYEIAGLSVDSRRLIFVSPEND
                     MTFLIVRSSDVPTYVEYGGADAGIVGKDVLMEQESDVYEPLDLGFGACRISVASLRGE
                     GSNDRLSSKIRIATKYPRISERYFNKRGIPVEIIKLYGSIELAPVVGLADRIVDLVET
                     GVTLKA"
     gene            complement(509..1777)
                     /gene="murA"
                     /locus_tag="VN647_06060"
     CDS             complement(509..1777)
                     /gene="murA"
                     /locus_tag="VN647_06060"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWG96629.1"
                     /translation="MDEILINGGKRLAGEVRISGAKNSALPILASTILSGGECVITNV
                     PRVVDVLTMGKLLGILGAKVSHEGNRAVIQADVIASTEAPYDLVKTMRASVLVLGPLV
                     ARWGEAKVSLPGGCAIGSRPVNLHLAGLSKLGADISIEHGYITAKAKRLRGGRIYRDT
                     PTVTGTENLMMAASLAEGVTMLENAAKEPEIVDLADFLVKRGARIHGAGTDVITIEGV
                     RELHGGDHEVIPDRIEAGTYMAAAAMTHGDVTATHCRPSHLEAVLMKLREAGAEVQEE
                     KDTVRLTMPAKLKGTDIRTLPFPGFPTDMQAQMVALMSLAEGTSVITETVFESRFMHV
                     EELWRMGADIRIEGNRLVVTGCKKLTGAPVMASDLRASAGLIVAGLAAEGMTQVQRVY
                     HLDRGYERIEEKLQALGADVQRRRATMTTH"
     gene            complement(1787..2677)
                     /gene="prmC"
                     /locus_tag="VN647_06065"
     CDS             complement(1787..2677)
                     /gene="prmC"
                     /locus_tag="VN647_06065"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWG96630.1"
                     /translation="MPAPIITGPKTIGALITWAQHSLVTAGSTNASQEALWLLAYALG
                     MKHHELASRRDQTVSGEGLVRAESVVSRRVAREPLQYILETQEFCGLDFHVNPAVLIP
                     RPETELLVQEALREGGFVEGGVLVDVGTGSGCVAVTLATILSGMRIFALDCSRDALTV
                     ARGNAERHGVNDKITWKEGDLLEPLREYNLVGTVDAIVSNPPYIADEVWGGLHPEVRD
                     FEPRVALVAGRRGTEFHERLLHEARPFLASGGLLVMELGQGQAPLVRQAAEQVGGYIG
                     LQTVKDEGGIERVMIARRAG"
     gene            complement(2680..3759)
                     /gene="prfA"
                     /locus_tag="VN647_06070"
     CDS             complement(2680..3759)
                     /gene="prfA"
                     /locus_tag="VN647_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWG96631.1"
                     /translation="METALLKRWESLASRFQELTDQLMDPSVATQPSLLHKLSKERTD
                     LEPAATLFGAYHEYAKQLKDAIGILADPSAGNELHKLAEEEKVELERQKTEIEEQARE
                     FLIPRDPRDDKSLVLEIRAGTGGDEAGLFAGELFRLYVKYAEKKGFKVDTVETSETGI
                     GGYKNIVALIEGKGAYSHFKYEAGVHRVQRVPVTEASGRIHTSTVTVAVMPEVDEVDV
                     QIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHLPTGMVVTCQDERSQLKNRTKAMRT
                     LRARIVEAEREKQEAEIAQNRKSQVGTGERSEKIRTYNFPQNRVTDHRVGMTLHKLEL
                     VMEGHLDEIVHALKAQQQQAETAKV"
     gene            complement(3859..4065)
                     /gene="rpmE"
                     /locus_tag="VN647_06075"
     CDS             complement(3859..4065)
                     /gene="rpmE"
                     /locus_tag="VN647_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWG96632.1"
                     /translation="MQKGIHPVYREATVHCACGNSFKTRTTIGDISVDICSNCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKGK"
     gene            complement(4162..>4818)
                     /gene="rho"
                     /locus_tag="VN647_06080"
     CDS             complement(4162..>4818)
                     /gene="rho"
                     /locus_tag="VN647_06080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWG96633.1"
                     /translation="EVTLIVLLIDERPEEVTDWQRQVKAEVISSTFDEPAQRHAQVAE
                     MVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAPPSGKVLSGGLDSNALQRPKRFFG
                     AARNIENGGSLTIMATALVDTGSRMDDVIFEEFKGTGNMEVHLDRRLADKRLFPAIDI
                     SQSGTRKEELLVDKDRLNKMWILRKVLSPLGTMEAMEFLMDKIGGTKNNQEFLQSMNR
                     "
BASE COUNT         1048 a         1469 c         1127 g         1174 t
ORIGIN      
        1 tgcgctttga gggtaacgcc ggtttcgacc aaatcgacga tccgatctgc caatcccact
       61 accggagcca attcaatgga gccgtataac ttgataatct cgaccggaat gccgcgctta
      121 ttgaaatagc gctcgctgat gcgaggatac ttcgttgcga tgcgaatctt ggacgacaac
      181 cgatcattgg agccctctcc ccggagcgag gcgacggaga ttctacacgc tccgaagccc
      241 aaatccaatg gctcgtatac gtcactttct tgctccatca agacgtcttt cccgacaatt
      301 ccggcatctg cccccccata ttccacataa gtcggcacat cgctggaacg aacgatgaga
      361 aaggtcatgt cattctcggg actgacaaat atcagtcggc gactatccac cgacaatccg
      421 gcaatctcgt atccggctcg tcggaagaga tccagtgcgg actctataag ttttcccttt
      481 gacagagcga tcgtcagcat aggctccctt agtgagtagt cattgtagcc cgtcggcgct
      541 gaacgtcggc tcctaaggct tgtaatttct cttcgatccg ctcatacccc cgatcaagat
      601 gatagacgcg ttggacctgg gtcatgcctt ccgccgccaa ccctgctaca atcagaccgg
      661 cactggcacg gagatcagac gccatgaccg gagcgccggt gagcttcttg cacccggtca
      721 ctaccaatcg gttaccttct atacgaatat cggctcccat ccgccacaat tcttctacgt
      781 gcataaaccg gctctcgaac acagtttcgg ttatgacact cgtgccttcg gccaggctca
      841 tcaatgcaac catctgtgct tgcatgtcgg tgggaaatcc cgggaaaggc aaggttctga
      901 tgtcggttcc tttcagcttg gccggcatgg tcagacgcac cgtgtctttt tcctcttgca
      961 cttcggcccc ggcctcacgc aacttcatca ggacggcctc aaggtggctt ggacggcagt
     1021 gcgtcgcggt cacatctcca tgggtcatgg cagcggctgc catgtacgta ccggcctcaa
     1081 tacgatctgg gatcacttcg tggtctccac catgcagttc gcgtaccccc tctatcgtga
     1141 tcacgtcggt tcctgcacca tgaattcgtg cgccacgctt gacgagaaag tcggccaggt
     1201 ccacaatctc cggctccttg gccgcatttt ccagcatggt aaccccttca gcaagtgacg
     1261 ctgccatcat gaggttttcg gtaccggtca ccgtcggggt atcacggtag atgcgtccgc
     1321 ctctcaaacg tttcgccttg gccgtgatat agccgtgctc gatggagatg tcagccccta
     1381 attttgataa tcccgctagg tgaagattca ccggcctgga accgatcgca cagcctccgg
     1441 ggagagagac tttggcttcc ccccaacggg caaccaacgg tccaaggacc aaaactgatg
     1501 ctcgcatggt cttgacgaga tcatacggag cttccgttga tgcaatcaca tcggcctgaa
     1561 taactgctcg gttaccctcg tgcgacacct tcgccccaag aatccccaag agctttccca
     1621 tcgtgagaac atcaacgact ctaggaacat tcgtaatgac acattccccg ccactcagaa
     1681 tggtcgaagc caagatcgga agagcggaat tttttgctcc actgatacga acttccccgg
     1741 cgagtctctt gccgccgttg atgagaattt catccatgcc gtggtcttaa cctgctcgcc
     1801 gcgcgatcat cacccgctcg ataccgcctt catctttgac ggtttgaagg cctatgtagc
     1861 ctccgacttg ttcggctgct tgccgcacaa gcggagcttg gccctgacca agttccatga
     1921 cgagcaatcc gccggacgcg aggaatggtc gtgcttcgtg gagcaatcgt tcatgaaact
     1981 ccgttcccct ccgcccagct accaacgcaa cccgcggctc aaaatcccgg acttccggat
     2041 gcaaaccgcc ccagacctca tcagcgatgt agggagggtt ggatacgatc gcatccacag
     2101 ttccgaccaa attatattcg cgcaagggct ccaaaagatc cccttccttc caagtaatct
     2161 tgtcgttgac tccgtgtctc tctgcattgc cccttgcaac agtcaacgca tccctggagc
     2221 agtccagagc aaaaatccgc atgccgctca gaatagtcgc caacgtgacc gccacacaac
     2281 cggagcctgt tcccacatcg accagcacgc caccttccac aaacccgccc tctcgaagag
     2341 cttcttggac gagaagttcc gtctccggcc gaggaatcaa caccgccggg ttcacatgaa
     2401 agtcgagacc acaaaactct tgtgtttcca agatgtactg tagcggttca cgcgccaccc
     2461 gtcgcgatac tactgattcg gcacgaacca gtccttcgcc tgataccgtt tggtctctcc
     2521 gactagccaa ctcgtgatgc ttcattccca acgcgtaggc caataaccag agcgcttctt
     2581 gagacgcatt cgtcgatcca gctgtaacca atgagtgttg ggcccaagtg atcagtgcgc
     2641 caattgtctt cggacctgta ataatggggg cgggcatcgt tatactttgg cggtctcagc
     2701 ctgttgctgt tgcgccttca gggcgtgaac gatttcgtca agatgacctt ccatcaccaa
     2761 ttccagcttg tgaagcgtca tgccgactcg gtgatccgtg acccgattct gtggaaaatt
     2821 gtaggttcga attttctcgc ttcgttctcc ggttcccacc tgagactttc tattctgagc
     2881 gatctctgct tcttgctttt cccgttcagc ctcgacaatc ctggcacgca aggtccgcat
     2941 ggctttggtc cgatttttca actgagatcg ctcatcctgg cacgtcacga ccatacccgt
     3001 cggcagatgc gtaatacgaa cggccgaata ggtggtgttt acactctgtc caccggcccc
     3061 agatgagcaa aacgtatcga tccgcaaatc cttcggatca atctgcacat ccacttcatc
     3121 aacctccggc atgaccgcca ccgtcacggt cgacgtgtga atgcgaccac tcgcttcggt
     3181 gaccggaacc cgctgtacac ggtgaacgcc ggcttcatac ttaaagtggc tgtaggctcc
     3241 cttgccttcg attaatgcga cgatgttctt ataccctcca ataccggtct cagatgtttc
     3301 gaccgtatca accttaaatc cttttttctc tgcatacttg acatacaagc gaaatagctc
     3361 tccggcaaat aagcccgcct cgtctccacc ggtcccagca cgaatttcta gcaccaggct
     3421 cttgtcgtcc cgtggatctc ttggaatcaa aaattccctc gcttgctcct caatttccgt
     3481 cttctgccgc tctaactcga ctttttcttc ttccgccagc ttgtgcaact cattgcctgc
     3541 cgacgggtcg gcgagaatcc ctatcgcatc ttttagctgc ttggcgtatt catggtaggc
     3601 tccaaagagc gtggcggctg gttccaagtc ggtccgctcc ttactcaatt tgtgcaacag
     3661 acttggttga gttgcaacgg acggatccat gagctgatca gtcaactcct gaaaccgtga
     3721 tgcgaggctc tcccatcttt tgagtaatgc cgtttccacc gtttcctacc taaggctttt
     3781 gcaggcacag caccacgaca aaaaaagaga ccctgcttca aacacgaagc agggtctctt
     3841 ttctacccag atgatgcgct acttaccctt cttcgcgtac ttctttttga accgctcaac
     3901 gcgtccttca gtatcgacga tcttttgtgt accggtgaag aacggatggc aattggagca
     3961 gatatcgacg ctgatgtcac cgatggtggt acgtgtctta aacgaattcc cgcaggcgca
     4021 gtgaaccgtc gcctcgcggt atacaggatg aatacccttc tgcatgacct acgactcctt
     4081 acccaagaat tgtaaacgcg cttggtcact tcctaagggg caacactgta tctgaaggaa
     4141 gaccaagaca caagcaccac attatcgatt catcgactgc agaaactcct ggttgttctt
     4201 ggtgcccccg attttatcca tgaggaactc catggcttcc atcgtcccca aggggctgag
     4261 caccttccgg aggatccaca tcttgttaag gcgatccttg tcaaccaaca gttcttcctt
     4321 ccgcgtgccg gactgactga tatcgatcgc cggaaacaga cgtttgtcag ccaagcggcg
     4381 atctaaatga acttccatat taccggtccc tttgaactct tcaaagataa cgtcgtccat
     4441 gcgactacct gtatctacaa gcgccgtcgc cataatggtg aggctcccgc cattctcgat
     4501 attgcgagcc gctccaaaga agcgcttcgg ccgttgtagc gcattggagt ccagaccgcc
     4561 ggagagcacc ttgccgctgg gcggtgcaat ggtgttatag gctcgtgcga ggcgggtgat
     4621 actatccaac agaatgacga cgtctttctt gtgctcgaca agccgcttcg ctttctccag
     4681 caccatttca gccacttgag catgtcgctg cgccggttca tcaaatgtgg aactaatgac
     4741 ctctgccttc acctgtcgct gccagtcggt gacttcttcc ggtcgttcgt caatcaacag
     4801 aacgatgagt gtgacttc
//
LOCUS       DATNDD010000252         5763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1293451, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000252 DATNDD010000000
VERSION     DATNDD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293451"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VN647_06085"
     CDS             <1..440
                     /locus_tag="VN647_06085"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWG96634.1"
                     /translation="TAEDFRLVDLEGKPQSLNQYRGKVVLVNFWATWCKPCTTEMPAM
                     QATYDKLREKGFVVLAINELEDDARVREHIKQYGHTFPVLMDRDNKVANQFGVFGLPV
                     SVFIDEKGVVQEYIKGGLLTEQVILDVVARIQKQEPVKAASLR"
     gene            519..1430
                     /locus_tag="VN647_06090"
     CDS             519..1430
                     /locus_tag="VN647_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWG96635.1"
                     /translation="MTSTDEIRFFFPAWGISLTLHGVAVGLAFAFAAQVKPVLKENLF
                     QWDVALVEAAKPESISEQAPSAVPPEQPSAKTLSQPRPKRATVVARAVKSLEQKIEPP
                     PPTINPIQTIEQKVENPQPREEPMEQRIVERTESKAEPVVEAKEPEPVTAPPPPHEPV
                     AVAADPASSHEVAAQQEAPAAPLPQESPVVPAPMEVARAPVAGSETKIDHRWLAESLW
                     RRVAELKRYPNQARMNGQEGKVILKAVIRSDGQLADVSVQKSSGHSVLDAAAIEAVKL
                     ACPLHMKHAIGKPQIVVSLPIVYSLAN"
     gene            1505..2782
                     /locus_tag="VN647_06095"
     CDS             1505..2782
                     /locus_tag="VN647_06095"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWG96636.1"
                     /translation="MRNFTATFIVMLSFFVSMHVRAEISEPTITLGSKGMTVHQVKNI
                     VGPSVQIDEQGIVSVAWVEEDKEIRTVLFARSEQPGGPLGRPVSVNQPTENVYYRQES
                     PALAVRGNEIFVAWSQTHPRITSDKPFSGELRLSKSMDGGRSFVPSVLVNDDRQVIQH
                     TFDSIQIAPNGTLHMAWIDGREGKKEPGTFVTRSTDHGRTVAQNLKVDDDTCVCCRTA
                     VATSADGTVYVAWRKIFEGNIRETVVSRSMDDGRTFSSSVVVGNDQWVYPACPHRPAS
                     LGVDRQGRLYVVWYTEGADEMPAIYLVYSDDQGKTFSTKKQLNRSKGTFPDHPQMAVD
                     PAGRIAVIWEEQSPVRREVVVSYSLDRGRSFNAPIKLNEKNGQTPTVAVNQHGTVVMG
                     WKEHAMPAHRLVIQTLQFPMENAMAERTESHGQ"
     gene            2772..3341
                     /locus_tag="VN647_06100"
     CDS             2772..3341
                     /locus_tag="VN647_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWG96637.1"
                     /translation="MGSKTRTVPSGLLLLGCVILFGAVDVPHRAATAETDPFAALRVS
                     RMAAGSPIASFDLKELDGRTVRSKELAGKVILLNFWATWCGPCKEEMPSLARLQSQFD
                     PAQFQVVTVTTDMHPQGIKQFLDHLGIRLPVLFDENEDVSRSFMVRGLPTTVLVTQEG
                     RAVGRAVGPRAWDGEESAALVRHVLEGMK"
     gene            3338..3643
                     /locus_tag="VN647_06105"
     CDS             3338..3643
                     /locus_tag="VN647_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96638.1"
                     /translation="MKRWFAPFVVGMILLYAALAVSAAGCLVMQTEESGHAHHAPSHA
                     AHSSLCAWICQINPTVSLHAAVPLLAFFVLVATQRLVDAAPQLHFISAVSRSRAPPQ"
     gene            3753..>5763
                     /locus_tag="VN647_06110"
     CDS             3753..>5763
                     /locus_tag="VN647_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWG96639.1"
                     /translation="MFELVRVSRWGYAMAVGLFVICAAASDEVFAQGAPSSDPSASPR
                     ERELRDQLKNILQELDELQQKKESEKPEAERPTIIKEKAEAASPEAATEAASEANPDF
                     DLADMSIVSKRLQKRPEGVSLSATVPSETDSQPTRTMKESMESLPGVVLRQANGPRDF
                     SIMIRGQGAKTSFAVRDIKIYEDGIQQTQSDGLSRLDMQDPWFMRSVEVTRGASSSLY
                     DNYALGGMVHFRTRRGRDIHGLETFFSGGSFGYQKYGVAIGQETEKFDIAMFGSHVAE
                     DGFIQHSNYNTQTINFNVRYKMDDRQNFYFKAITNWLNTKVPTRLTQGQFLTDERQAG
                     GTRALCTPGTYNGGCADALLLDQSRVDRRTIIGGIYERQIDANTVLTVEADYDVKDIQ
                     QSFSQIFENTNPNYKSYADLRHDGRLGVMPLKSYVGFFVNKMEQKGNTFQNLADGFGT
                     KGNLIQNSRGTIFNIGGRIREELEFYPNWILAVGLGFEQSRLSVDATNYDQNGTLASR
                     VSANRTFSNWAPEGSLTWKPSADYRYWLRASTGYGIPQFSNLLRDPVTGLPGSNFSLK
                     PQKNLNLEIGTEMRLHPTLLVHVVGFYTFFKDEIITQVISGNNTASVNADSSRYRGVE
                     LFADWRPVTGLRLSGAYTHINSEYINFSDRTAAGFIVRDGKHVPNV"
BASE COUNT         1331 a         1533 c         1691 g         1208 t
ORIGIN      
        1 cgaccgcgga ggattttcga ttggtcgatt tggaaggcaa gccgcagagc ctgaaccaat
       61 atcgcggcaa agtcgtgctg gtgaattttt gggccacctg gtgcaaaccc tgcacgacgg
      121 aaatgcccgc catgcaggcc acgtacgaca agctccgcga gaaggggttc gtggtgctgg
      181 cgatcaatga actggaagac gatgccaggg tgcgtgagca catcaagcag tacggccata
      241 cgttcccggt gctcatggat cgagacaaca aagtcgcaaa tcagttcggg gttttcgggt
      301 tgcctgtgag cgtgttcatc gatgagaagg gcgtcgttca agaatacatc aagggcggcc
      361 ttctgaccga gcaagtcatc ttggatgtgg tcgctcgtat tcagaaacaa gaaccggtga
      421 aggccgcatc cttgcgctag atgatcagta tgacggttat cgtgaaacgg ccggagtggg
      481 cgaaccgatc aggggtaagt ggaaaggcag aagacaatat gacatcgaca gatgaaatcc
      541 gtttcttttt tcccgcttgg ggaatttcgc tgacccttca tggtgtagcg gttggcttag
      601 catttgcttt tgccgcacag gtcaagcccg tcttgaagga gaacctgttc cagtgggacg
      661 tggcattggt ggaagcagcg aagccggaat ccatatcgga acaagcgccg tcagccgtgc
      721 ctccggaaca gccatcggcg aagaccttgt cgcagccacg tcccaaacga gcgacagtgg
      781 tagcacgggc ggtaaagtcg ttggagcaga agatcgagcc gcctccaccg acgatcaacc
      841 cgatacaaac gatcgagcag aaagtggaaa atccccagcc acgtgaagag ccgatggaac
      901 aacgtattgt cgagagaact gaatcaaagg ctgagccggt cgtagaagcg aaggaaccgg
      961 agccggttac ggctcccccc ccgccgcatg aaccggtagc ggttgcagca gatcctgcct
     1021 catcgcatga ggtcgcggcc caacaagaag caccggcagc acccctgcca caggaatcgc
     1081 ccgtggttcc tgctccaatg gaggtggcca gggctccggt ggccggttcg gaaacgaaaa
     1141 tagatcatcg ctggttggcc gagtcgctct ggcgacgggt ggcggaactc aaacgatatc
     1201 cgaatcaggc ccgcatgaac ggccaagagg ggaaagtgat cttgaaagcc gtcatccgat
     1261 cggatggaca gctggcggat gtatccgttc agaaaagttc aggtcacagc gtgctcgatg
     1321 ccgcggcgat cgaggcggtg aaactggcct gtccgctcca tatgaaacat gccatcggaa
     1381 agcctcaaat cgtcgtgagt cttccgatcg tgtatagctt ggccaattag tgaatcgaat
     1441 gagggtcaaa cgacgtcatg aatgcagctc attcaagatc atgcaaggtc atgaggggta
     1501 agcgatgcga aacttcactg ctacattcat cgtgatgctg tcatttttcg tgtcgatgca
     1561 tgtgagggcc gagatttccg agccgaccat cacccttgga tctaaaggta tgacggttca
     1621 ccaggtgaag aacatagtcg gcccctcggt acagatcgac gagcagggga tcgtctcagt
     1681 tgcatgggtg gaagaagaca aggaaatccg caccgtcctc tttgcgagat ctgaacaacc
     1741 cggtgggccg cttggaagac ccgtctctgt gaaccaaccg accgagaatg tctattatcg
     1801 ccaggaatcc ccggccttag ccgtccgtgg gaatgaaatc ttcgtcgcgt ggtcgcagac
     1861 ccacccgagg atcacgtccg ataagccgtt ttccggcgaa ctgaggctga gcaagtccat
     1921 ggacggcgga cgctcattcg tgccgtccgt cctggtgaac gatgatcgcc aagttatcca
     1981 gcacacgttc gattcgattc agatcgctcc gaatggcacc ctgcacatgg cctggatcga
     2041 cggccgcgaa ggcaagaaag aaccggggac gttcgtgacg agatcgacgg atcatggccg
     2101 cacggttgcg cagaacctca aggtcgacga tgatacgtgc gtctgttgca ggacagccgt
     2161 cgcaacgtcc gccgacggaa ctgtgtacgt ggcatggcga aagattttcg agggaaatat
     2221 tcgagagacg gtcgtatctc gatcgatgga cgacggccgg acgttttctt cttcagtcgt
     2281 cgtcggcaac gatcagtggg tgtatcccgc ttgcccgcat cgtccggcat ccctcggcgt
     2341 cgatcgacaa gggcgactgt atgtcgtttg gtacacggag ggcgccgacg agatgcccgc
     2401 aatttatctg gtctattccg acgaccaggg gaagacattt tccaccaaaa aacagctgaa
     2461 ccgctccaaa gggaccttcc cggaccaccc gcaaatggcg gtcgatccgg cagggaggat
     2521 tgccgtgatc tgggaagaac aatcgcctgt ccgtcgagaa gtcgtggtca gctattcgct
     2581 cgatcgcggc cggtcattca acgctccgat caaattgaac gagaagaacg gacagacgcc
     2641 gacggtggcg gtcaatcagc atggtacggt cgtgatgggc tggaaggagc atgcgatgcc
     2701 ggcgcatcgc ctcgtgatcc aaaccctgca atttccaatg gagaacgcga tggctgaaag
     2761 gaccgaaagc catgggcagt aaaacccgca ctgtgccgag cggtctattg ctcctcggat
     2821 gtgtgatcct gttcggcgcc gtcgatgttc cgcaccgtgc ggccactgca gagaccgatc
     2881 cctttgccgc gctcagagtc agtcgaatgg cggccggttc tccgatcgca tcgttcgact
     2941 tgaaggagct tgacggtcgg acggttcgct caaaggaatt ggctggaaag gtcatcctcc
     3001 ttaatttttg ggcgacctgg tgtgggccgt gtaaagaaga aatgccgtcg ttggcccgac
     3061 ttcaatcgca gttcgatccc gcacagtttc aggtagtgac cgtcacgacg gatatgcatc
     3121 cacaagggat caagcagttt ctcgatcatc tgggcatccg actacccgtg cttttcgatg
     3181 agaatgaaga tgtgtcgagg tcctttatgg tcaggggtct gccgacgacc gtactcgtta
     3241 cgcaagaggg tcgagccgtc ggtcgagccg tcgggccgcg ggcctgggac ggcgaggagt
     3301 cggccgcgct ggtgcgacat gtgcttgaag gaatgaaatg aagcgttggt tcgcgccctt
     3361 cgtcgtcggg atgatcctgc tctacgccgc gctggcagtc agcgcagcag gctgtctcgt
     3421 gatgcagacg gaagagtcgg ggcatgctca ccacgctccg tctcatgccg ctcactcctc
     3481 gctctgcgcc tggatttgtc agattaaccc gacggtcagc ctccacgccg ccgtgcctct
     3541 cctcgcgttc ttcgtcctcg tcgcgacgca gcggttggtt gatgcagcac cacaactcca
     3601 tttcatttcg gcggtttctc gatctcgggc tcccccgcag tgatcctttt ctagatcgta
     3661 tcggtcgtag gcggttaccg gctcggcagc cgatgcggct gtgtgtcgga ctatgccctt
     3721 caacaatctg ctcgagcgag gagtcggcga tcatgtttga attggtgcgt gtttcccgat
     3781 ggggatatgc gatggctgtc ggtctgtttg tgatatgtgc ggctgcaagt gacgaggtgt
     3841 ttgcgcaagg tgcgccttct tcagatccat ctgcgtcacc gcgcgagaga gagctgcgcg
     3901 atcaactgaa gaatattctc caggaactgg atgagttgca gcaaaagaaa gagagtgaga
     3961 aacccgaagc ggaacgcccg acgattatta aggagaaagc tgaggcggct tcaccggagg
     4021 ctgcaactga agccgcatcg gaggcaaacc cggatttcga tctggctgat atgagcatcg
     4081 tcagcaagcg tctccaaaaa cgaccggaag gcgtgtcact ttctgcgacc gttccttcgg
     4141 aaacagattc gcagccgacc agaaccatga aggaatcgat ggaatcgctt cccggcgtcg
     4201 tgttgcggca agccaatgga cctcgcgatt tcagcatcat gatccgcggg caaggcgcca
     4261 agacgtcttt cgcggttcga gacatcaaga tctacgagga tggaattcag caaacacaat
     4321 cggacggact ttcacgtctc gatatgcagg atccctggtt catgcgcagc gtcgaagtca
     4381 ctcgcggcgc atcttcttcc ttgtacgaca actatgcatt gggtggaatg gttcacttcc
     4441 gcaccagacg gggaagggac atccacggat tggagacctt cttctcggga ggatcctttg
     4501 ggtaccagaa atacggcgtc gccatcggcc aagaaacaga gaaattcgat attgcgatgt
     4561 tcggcagcca tgtggcggaa gatgggttca tccagcacag caactacaat acgcaaacga
     4621 ttaacttcaa cgtccgctac aagatggatg accggcaaaa tttctatttc aaagccatta
     4681 ccaactggct caatacgaaa gtgcccaccc ggcttacgca gggacagttt ttgacggatg
     4741 aacggcaggc aggtggtacg cgagcgctct gtacacccgg cacctacaac ggcggctgtg
     4801 ccgatgcgct tttgctggat caaagccggg tcgatcgtcg cacgattatc ggagggatat
     4861 acgagcggca gatcgatgcg aataccgtcc tgacggtgga agccgattat gacgttaaag
     4921 acatccagca atcattttcg cagatcttcg aaaatacgaa tcccaactac aagtcctatg
     4981 ctgacttgcg gcatgacggt cggttgggag tgatgccgtt gaaaagctat gtcggtttct
     5041 ttgtgaataa gatggagcag aagggcaaca cctttcagaa tcttgcggat ggcttcggaa
     5101 caaaagggaa tctgattcag aacagccgcg gaactatctt caacatcggc ggccgtatcc
     5161 gggaggaatt ggagttttac ccgaattgga tcttggcggt cggactcggg tttgaacagt
     5221 cccgtctcag cgtggatgcc acgaactatg atcagaacgg gacgcttgcc tcacgggtca
     5281 gtgccaatcg aacgttctcc aattgggcgc ctgagggatc gctgacttgg aaaccgtcgg
     5341 cagactaccg gtattggctg cgagcctcga caggctatgg cattccgcaa ttcagcaatc
     5401 ttctgcgaga tcctgtgacg gggcttccgg ggagcaattt cagcctaaaa ccgcaaaaga
     5461 acctcaatct ggaaatcggc acggagatga ggctgcatcc aaccttgctc gtccatgtgg
     5521 tcggcttcta caccttcttc aaagacgaaa tcatcacgca agtgatctcg ggaaataaca
     5581 ccgcctcggt caatgcagac tcttcgcggt atcgcggtgt cgaactattc gccgattggc
     5641 ggcctgtgac aggtctacgg ctatccggcg cctataccca cattaattcg gagtacatca
     5701 acttttccga tcggaccgca gcggggttca ttgtccgtga cggcaaacac gtgcccaatg
     5761 tgc
//
LOCUS       DATNDD010000253         4787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_293715, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000253 DATNDD010000000
VERSION     DATNDD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293715"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1028
                     /locus_tag="VN647_06115"
     CDS             222..1028
                     /locus_tag="VN647_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HWG96640.1"
                     /translation="MDSQADDFEQTELPYQVRIENFEGPLDLLLHLIKRNEINIYDIP
                     VAMIAQQYLEYLEAMEELNLNVAGGFLVMAATLLQIKSKMLLPVEETADDDEDGPDPR
                     EELVRRLLEYKAFKEAARQLDDQEKMWSEIFWREQVPPVEEVEEDLPLENVSLFDLVD
                     ALKEVLERNPSSRLIEIAPDNLTVRERMNLILETLEGKDSVSFAALFGVSSQRMVVVV
                     TFLALLELMRLRVARVFQAEAFGPILVSRTFSLVPDPAELDDVDVEWRGA"
     gene            1025..1822
                     /gene="scpB"
                     /locus_tag="VN647_06120"
     CDS             1025..1822
                     /gene="scpB"
                     /locus_tag="VN647_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWG96641.1"
                     /translation="MTPLTADAVHDVPEADAMNIGEANGSPHDQMADDDRVQSMGELQ
                     AILEALLFVSSEPLSLARLVAVMGNVPKVEVEEALRNLGQVFEQEGRGVRLAAVAGGY
                     RLVTKQDYAPWIKRLDKAKTAAKLSRSALESLAIIAYKQPLVRSEIEEIRGVETSGVL
                     RTLLERKLVRIVGRKEVPGRPIMYGTTKFFLEHFGLSDLSQLPPLREFKELGEAEQLL
                     FPMDGRDVSSGEVSIEALEAGMEPSVDAELQTSVPLDEVLDPLPTAQ"
     gene            complement(1916..2800)
                     /locus_tag="VN647_06125"
     CDS             complement(1916..2800)
                     /locus_tag="VN647_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96642.1"
                     /translation="MLGTLSLMAIGCVGPRPTTPIHEASLGTVFLENVPDTSFQAAHP
                     IKLPETTVADILRGVHTKEKTGLLLLLGKALKSTNLNDIRTFSEDDIAFLTPHIATAL
                     AQATPNQRVGFHIYPTPEISQQPKVGQNRETTSGYLFADGLSLHFTLTQYRYRPGKRS
                     NASQKEPRQLPDTDGLRDREVTFLPEAAVRPDVYDRSSWIGKSEDRSLAIDYQLLARV
                     LATPPPSAPVAQPVPAASAPVPQQPQSIPAQAPDGKNAADLQAFKEELKALQKKMDEQ
                     EAELRRLKNPPSRKKTAP"
     gene            3506..>4787
                     /gene="urtA"
                     /locus_tag="VN647_06130"
     CDS             3506..>4787
                     /gene="urtA"
                     /locus_tag="VN647_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HWG96643.1"
                     /translation="MDQKAPQDSQNVKETVAPTDETGSSRREFLGQSGRVAAGIGVAA
                     LLGNLGNYALSYAAGGPPIKIGVLHSLSGTMAISEVSLRDVVLMAVEEINKAGGVMGR
                     QIEAKVVDPASNWDLFAEKAKQLLLEDKVSVVFGCWTSVSRKSVLPVFEKNNGLLFYP
                     VQYEGEECSRNVFYTGAAVNQQAAPAVEYLMSPEGGNYKKFYLLGTDYVYPRTTNKIL
                     RAMLLAKKVPAANIEEEYTPFHHQDYQTICGKIKKFAAGGGAAVVSTINGDSNVPFYK
                     EFGNQGLRAEDAPIMAFSVAEDELRGMDTSALVGHLAAWNYYQSVDTPQNKKFVANFK
                     AYCKRNNLPDGENRVTDDPIEAAYFGVYVWKQAVEKAGSTEVDKVRKAVYGQKFLAPG
                     GQIMMDEANQHTHKPVLIGEILKNGQFKTIWRSKG"
BASE COUNT         1130 a         1141 c         1426 g         1090 t
ORIGIN      
        1 cttttcaccc aaacaaagcg gcttgaatca gctcctcctg ccggattttt tcaggcttgg
       61 aatggaccgt atgccctcac cttggtcttt ctcgtcgtgg ctgccttact tgtagcttat
      121 gccgtaagac tcaaatgaag agcacttaaa aagtacgcga atcatcccca atatatagaa
      181 tcagacttgc ccgttacccc ataagttgat ataacgaggc cgtggactca caggccgatg
      241 attttgaaca aaccgagctt ccgtaccagg tccgcatcga aaattttgag ggcccactcg
      301 atcttttgct ccatctcatc aagaggaatg agatcaatat ttacgatatc ccggtcgcga
      361 tgattgccca acaatatctc gagtatctgg aggcaatgga agaactgaat ctcaacgtgg
      421 cgggggggtt tttggtcatg gcggcgacgt tattgcagat caaatccaaa atgctgctgc
      481 cggtggagga gacggccgac gacgatgaag acgggccgga cccgcgcgaa gagctcgttc
      541 ggcggttgct tgaatataag gctttcaaag aggcggcacg tcagcttgac gaccaggaga
      601 agatgtggag cgagatcttt tggcgtgagc aagttccacc ggttgaagag gttgaggagg
      661 atcttccgtt agagaatgtc tcgctgtttg atctcgttga cgcactcaag gaagtactgg
      721 agcggaatcc gagcagccgg ctcatcgaga ttgctcccga caatctcacg gttcgcgagc
      781 ggatgaacct catcctggaa acgctcgaag gcaaagactc ggtctcgttt gccgcgctgt
      841 tcggggtatc gagccaacgc atggtggtcg tcgtgacgtt cctggcgtta cttgaattga
      901 tgagattgcg ggttgctcgt gtattccaag cggaggcatt cggaccaatt ctggtttcac
      961 ggacgttctc cttggttccc gatccggcag agcttgatga tgtcgatgta gaatggaggg
     1021 gagcatgact ccactgacgg cggatgcggt tcatgatgta ccggaagccg acgcgatgaa
     1081 tattggagaa gcgaatggat cgcctcatga tcagatggcc gacgacgatc gggtgcaaag
     1141 tatgggcgaa ctccaagcga ttctggaagc actgctcttc gtttcttcag aaccgctgtc
     1201 cttggcgcgc cttgtggccg tgatgggaaa tgttccgaag gttgaagtgg aagaggcgtt
     1261 gcgaaatctc ggtcaggtat ttgagcaaga agggcggggg gtgcgactgg ccgctgtcgc
     1321 gggcgggtac cgtctcgtca cgaagcagga ttatgccccc tggatcaagc ggttggataa
     1381 ggccaagacg gcggcaaaac tgtccagatc cgccctggaa tcgttggcga ttattgccta
     1441 caagcagccg ctggtgcggt cggagatcga agaaatccgt ggggtggaaa cctccggtgt
     1501 cttgcgcaca ctgttggagc gaaaactggt ccgaattgtc gggcgcaaag aggtaccagg
     1561 acgtccaatc atgtacggga cgaccaaatt ctttcttgaa catttcggct tgagcgatct
     1621 ctcgcagctg cccccactgc gggaattcaa ggaactgggt gaggcagagc aattactctt
     1681 tccgatggac ggtagagacg tttcatccgg ggaggtgtcg atcgaggcgc ttgaggcagg
     1741 catggaaccg tcagtggatg ctgaactgca aacgtctgtt cctctcgatg aggtattgga
     1801 tcctctccct accgctcagt aaccaacgaa cattcgtatg aaatcagaac ggtagactcg
     1861 ttgcggacgg ctccacgtcc aggcctggtc cttcacatcc gcttgcggag gccggctaag
     1921 gtgcggtttt tttcctagat gggggattct tgaggcgccg gagctcggcc tcctgctcgt
     1981 ccatcttttt ctgaagtgct ttcagctctt ccttgaaggc ttgcaggtca gccgcattct
     2041 tcccatctgg tgcttgagca ggaatcgatt ggggttgctg tggaaccggc gctgacgccg
     2101 ccgggaccgg ctgtgcgacg ggagcggaag gtggaggtgt tgcaagtacc ctcgccaaca
     2161 gttggtagtc aatagccagc gaccgatctt cggattttcc aatccagctc gaccgatcgt
     2221 atacgtcggg acgcacggcc gcttccggta ggaacgtgac ctctcggtca cggagaccgt
     2281 cggtgtccgg gagctggcgt ggttcctttt gactggcgtt ggaccttttt ccggggcgat
     2341 atcggtactg ggtcaacgtg aagtgcagcg acaagccgtc cgcgaataga tagccggacg
     2401 tggtttctct attttggccg accttcggct gctgtgaaat ctccggtgtg ggatagatat
     2461 ggaaacccac gcgctgattg ggagtcgctt gagcgagggc agtggcaata tgtggggtca
     2521 ggaacgcgat atcatcctcg gagaaggtgc gaatatcgtt caggtttgtt gacttcaagg
     2581 ccttgcccag cagcaataag aggccggttt tttcttttgt gtgtacacca cgtaagatat
     2641 ctgcgacagt cgtctcaggt aatttgatgg gatgtgcggc ttggaaggaa gtatcaggca
     2701 cattctcaag gaataccgtg ccaaggcttg cctcgtgaat cggtgtggta gggcgagggc
     2761 cgacgcaacc gatggccatg agactcaggg tgcccagcat gagaaggctc agagccaacc
     2821 taaaagaagg gcgctgatgg aaattcatga tgttgcgttg ctgaatgaag ttcacgcgaa
     2881 cgtagtgtaa caggaacatc cgacaaaaac aaaaagatcg tcgttcagtt tgcatcagat
     2941 cgtgtggcca ggggaaatgt tgcccacgaa gtttgtggga ccgtgatctt ggcgagcgtg
     3001 agtcgacgtg tgtcagatgg agcctttctc aacgacacga gagtgagtgg attgctcggc
     3061 tgaggatggc tccctctcta ggtttgcaga cgaatcatct ctatcgatag cggacatcac
     3121 cttgacggaa cgaacggttt ttgtatacaa tcttgtcgcc ctgcggagga aatgaattga
     3181 atgataatta gacgcaagtg gggcaggtca ttcggtaacg cccttcccgt acaacgctcg
     3241 ccacacggaa acagccatcc ttgtccccct caggttagtg cagatagttt tcactggctc
     3301 tttgacattc ctaagaaagg agggagatga gtctgttcgc gagccaggta gttcggcgta
     3361 acgccgtgca atagtcgaat cgaagaaact tatctctcta aagactaatg caaggcggcc
     3421 gattgtttct gagagtaggt tccaagcaaa atgcgcatca accggaagtt tgcaactcac
     3481 ggcatacgaa aaggagtgat gcaacatgga ccagaaagcc ccacaggatt cacagaacgt
     3541 caaggaaaca gtagccccta ccgatgaaac cggatcatcg cgaagagaat tcctaggaca
     3601 gagtggacgc gtggcggcgg gaatcggtgt cgcggcgcta ctgggaaatc tgggtaacta
     3661 tgctttgtcc tatgcggccg gtggtccccc gattaagatc ggcgtactgc attctttgag
     3721 tggtacgatg gcgatcagcg aagtgtcgtt gcgcgatgtg gtgctgatgg cggtcgaaga
     3781 aatcaataag gcgggcggtg tcatgggacg gcagatcgaa gcaaaggtcg tcgatcccgc
     3841 gtcaaattgg gacctgtttg ccgaaaaagc gaaacagctc ctcttggaag ataaggtgtc
     3901 ggtggtgttc ggctgttgga catcggtcag caggaaatct gtgctgccgg tttttgagaa
     3961 gaacaacggg ttactcttct atcctgtcca atacgaaggt gaggaatgtt cacgcaatgt
     4021 gttttacacc ggggcggcgg ttaatcaaca ggcggcgccc gccgtggaat acttgatgag
     4081 ccccgaaggg gggaactaca agaagtttta tctcctaggc accgactacg tgtatcctcg
     4141 cacgacgaac aagattctgc gtgccatgtt gttggcaaag aaagtgcctg ccgccaatat
     4201 tgaagaagaa tataccccat tccatcacca ggattatcaa accatctgcg gcaaaatcaa
     4261 gaagtttgcc gcgggtggcg gagctgccgt cgtcagtacc atcaatggtg acagcaatgt
     4321 gccgttctat aaggaattcg gtaaccaggg gctgcgcgca gaagatgcgc cgatcatggc
     4381 cttcagcgtc gccgaagacg aactgcgcgg aatggatacc agtgcgcttg tcgggcattt
     4441 ggcggcgtgg aactactatc aaagcgtgga tacgcctcaa aacaagaagt tcgtggcgaa
     4501 cttcaaggct tattgcaaga ggaataatct gccggatgga gaaaaccgag ttaccgacga
     4561 tccgatcgag gccgcgtatt tcggcgtcta tgtgtggaaa caggcggttg aaaaggctgg
     4621 ttctaccgag gtggacaaag tccgcaaggc tgtctatggg caaaaattcc tggcccccgg
     4681 cggccagatc atgatggatg aagccaacca gcacacgcat aaaccggtgc tgatcggcga
     4741 aatcctgaag aacggtcaat tcaagaccat ttggcgttcc aaaggat
//
LOCUS       DATNDD010000254         3961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2558984, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000254 DATNDD010000000
VERSION     DATNDD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558984"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN647_06135"
     CDS             complement(<1..524)
                     /locus_tag="VN647_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96644.1"
                     /translation="MTEFHPNALCFGEEFSAKGIPCLVHVEGHGLTMTISSDSEAGER
                     RSESVPFSELTVSAGGLDHDQLVATWTGPTGQRMLYLKNPDVIRAFRQAAPDHLTIPL
                     ERAAEQVRRVRQRHRIVWGIVGGALLASVLGLWFGSDLLVELAVDRIPIEWERKLGES
                     AYHDFLARQEVMKEG"
     gene            complement(802..1485)
                     /locus_tag="VN647_06140"
     CDS             complement(802..1485)
                     /locus_tag="VN647_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HWG96645.1"
                     /translation="MKSEILYPGAFPMARVELAEGEHIKAESGAMVACSPSIDIESKM
                     EGGFLGALSRKFLTGEKFFFQTLRASRGAGEVLLAPTVPGEIVVLDLDGVNEYMVQKD
                     GFLAGADGVTIDSQMQSMTRGLLGGEGFFILKMSGKGMLLLNSFGAVHKIELKPGQEY
                     IVDNSHLVAWSTTTTYNIEKATSGWVASFTSGEGFVCRFRGPGLVFIQSRNPGGFGAW
                     VRQFIPVSE"
     gene            complement(1638..3122)
                     /locus_tag="VN647_06145"
     CDS             complement(1638..3122)
                     /locus_tag="VN647_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWG96646.1"
                     /translation="MGHSPRPFFEDLSVRKGATFPTAVPTQIVSNEEFFPICQTAEQA
                     CVERVTRELVQKGAAQRGLTRRDFLKTTGGTAAALLAMNDAFGRFFNIGEIELFETAA
                     FAEQQGDPYFIFDVQTHYVSSHYDPSEAESGRKGAVSKQALLSLRKHIREAGLNPKLA
                     EDRGTLDDLSWKNFVKEVFFDSETTVGLISTPPGPYPQEAVVPPKEMAHIRDEINRLA
                     GSQRMLAHGLATPQLGAADLEFMAMQAETLKVDAWKCYTGSCPKGFDRGWRMDDERLA
                     YPMLEQARKLNIKRICVHKGLPLGPVPDYNHPRDLIKAAKDFPDLNFLVYHSGFRGTA
                     SIDQIFAKTGEIPWTTEFCRMKEAEPSISNIYMELGSTFAQLVTTYPLICAHLLGQII
                     RSFGVDHVLWGTDSIWYGTPQWQIEAFRRFQIPEELIEKHQYQPLTRRVKERVFGLNS
                     AGVFGVDVEAKRREVPNDALGRLRMSYLEEGPEPSRRVYGWVAG"
     gene            complement(3222..>3961)
                     /locus_tag="VN647_06150"
     CDS             complement(3222..>3961)
                     /locus_tag="VN647_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96647.1"
                     /translation="AINDDIAVASGLLVRSDFDGKYLGRISKFLRPLDQGELSMRWPM
                     QSELVVASKMYDAQLKAARAEEQPVYTMVASALPLPMQRRLNDYADYYDASYKAAGEG
                     KYGSLPKWKNYIHFPASGLTDYFTDPIENIVGLEPLAPWDRYNGFVVDTDASLRLASL
                     QAWLRRGSLDGDLLTRIAKAGQNFYDPYTGLPMLVNMKKGLLYSVGHDGKDQDADPRS
                     DVVVEIPAVQTSAAQAKSSIASSNKSR"
BASE COUNT          850 a         1205 c         1031 g          875 t
ORIGIN      
        1 ccttctttca tgacctcctg ccgagcaagg aaatcatgat aggcagactc gcccagcttt
       61 cgctcccact cgatcgggat tcgatcgacg gcaagttcca ccagtaggtc actcccaaac
      121 cagaggccca acaccgaggc cagaagcgcc cctccaacga taccccacac gatccgatgc
      181 cgttgccgaa cccggcggac ctgttccgcc gctcgttcaa gcggtatggt caagtgatca
      241 ggcgccgctt gtcggaaggc acgaatcacg tcggggtttt tgagatacag catccgctgc
      301 cctgtcggtc ccgtccatgt cgcgacgagc tggtcatgat ccaatccacc tgccgaaacg
      361 gtcagttctg aaaatggcac cgattccgaa cggcgctcgc ccgcttcaga atccgaggaa
      421 atagtcatcg taagcccatg tccttcgaca tggacaagac acggtatccc cttggcagag
      481 aattcctcac cgaaacagag cgcattgggg tggaattcgg tcatacgtgg ttctcgattg
      541 attcattggc ggcggaatgt tctcgccgcg gatgctcaaa acggccacca acaaggccgc
      601 aggcgaggcg cacggcgcga tgaagaaaga gcgccacgtc tgtgcgcgcc ggcgagacgg
      661 tgagccggca gtgtctttgg ccgaggcgtg ctagtttcca ccctcccacc ccgagcggcc
      721 aagacagctc cttcctcgta tggggtacgt tgaggattgt ttcgaaccga gaacgaagtt
      781 ggcgggcatt ttcagcatcc gctattcgga gacaggaatg aactgtctga cccatgctcc
      841 gaagccgcct ggattgcggc tctgaatgaa cacaagaccc ggtccgcgga atcggcaaac
      901 aaacccttcg cctgaggtga agctggccac ccatccggaa gtggcctttt cgatgttgta
      961 cgtggtggtg gtggaccaag ctaccagatg gctgttgtcc acgatatatt cctgacccgg
     1021 tttcaactcg atcttgtgaa cggctccgaa gctgttcaga aggagcatcc ccttcccgct
     1081 cattttgaga atgaagaacc cctccccacc caacaatccc cgggtcatac tctgcatctg
     1141 actgtcgatc gtaaccccat cggcacccgc caggaatcca tccttctgca ccatatactc
     1201 gttgacgcca tccagatcga gaacaactat ctcgcccggc accgtcggcg caagcaacac
     1261 ctcgccggca ccgcgactgg cgcgcagcgt ctggaagaaa aacttctctc ccgtcagaaa
     1321 ctttcgtgag agcgctccga gaaatccccc ttccattttg ctctcgatgt cgatgctcgg
     1381 cgagcaggcc accatcgcgc ctgattcagc cttgatatgc tccccctcgg ccaactcaac
     1441 acgcgccatc ggaaacgccc caggataaag aatttcgctt ttcattattt ctcttccttc
     1501 ctcttctctc tatcatccat gggcggagct ggtcgatgtc tcgttgcgcg catgcaccga
     1561 gcaccgaccg ccgcccagct ttataatcga tcctgcgtgc gcggtgcgcg agcagaagac
     1621 acgacctgct ccgcccttca tcccgccacc catccataca ctcttcggct cggctccggc
     1681 ccctcttcca gatagctcat tctgagacga cctagtgcgt cattgggaac ctcgcgccgt
     1741 tttgcttcga catcgactcc gaacactccg gccgagttta atccaaaaac ccgttccttc
     1801 acccgtctcg tcagaggctg atactgatgt ttctcgatca attcctcggg aatttggaat
     1861 cgccgaaacg cttcgatctg ccattgcggc gttccatacc agatggagtc tgtcccccac
     1921 agtacgtgat caacgccgaa cgagcggata atctgcccca gcagatgcgc gcagatcaac
     1981 ggataggtcg tcaccaattg cgcgaaggtc gaacccaact ccatatagat gttggagata
     2041 ctcggctccg cttccttcat ccggcagaac tcggtggtcc acggaatctc gccggtcttc
     2101 gcaaatatct gatcgatcga tgccgtgcct cggaacccgg agtgatacac caaaaagttg
     2161 agatccggga agtccttggc cgccttgatc agatctctgg gatggttata gtccggcaca
     2221 ggcccgaggg gcagtccctt gtgaacgcat atccgtttta tattgagctt tcgcgcctgt
     2281 tccaacatcg gataggcgag gcgctcatca tccatccgcc agccccggtc gaaccccttc
     2341 gggcaggaac cggtatagca tttccaggcg tcgaccttga gggtttcagc ctgcatcgcc
     2401 atgaattcca aatcagccgc ccccagctgc ggcgtcgcca aaccgtgggc cagcatccgt
     2461 tgcgaaccgg ccaaccgatt gatctcatcg cggatgtggg ccatctcctt cggcgggacg
     2521 acggcttctt gcggataggg cccgggcggc gtgctgatca agccgacggt ggtctcgctg
     2581 tcgaaaaaca cttctttcac aaaatttttc cacgagagat catcgagagt tcctcgatcc
     2641 tctgcgagct tcgggttgag tcccgcctca cgaatgtgct ttctcagcga cagcagtgcc
     2701 tgtttggaaa cggcgccctt ccgaccggat tcggcctcag agggatcgta gtgggaactg
     2761 acgtagtggg tttggacatc gaagataaaa tacggatctc cctgctgctc ggcaaacgcc
     2821 gctgtttcga acagctcgat ttctccgatg ttgaaaaatc gaccgaatgc gtcgttcatg
     2881 gccagcaacg cagccgccgt accgccggtg gtctttagga agtctcgacg cgtcaatcca
     2941 cgttgagctg cccccttttg caccaattct cttgttaccc gctcgacgca agcctgttcg
     3001 gctgtttggc aaatcgggaa aaactcctcg ttggatacga tctgcgtcgg cacagcggtt
     3061 ggaaacgtcg cacccttcct cacggagaga tcctcaaaga atggtcttgg tgagtgtccc
     3121 atgatatacc tcttcatcta tgcaaggccc actgattgtg tgaaatactc caagcggctg
     3181 tcaagcaatg ccggagcagg cctgaagtag cggggaaagg cttatctgga cttgttggat
     3241 gaagcgatcg atgattttgc ctgagcggca gacgtttgta cggcaggaat ctctaccacc
     3301 acatccgacc gaggatcggc atcttgatct ttcccgtcat gtccaacact gtagaggagc
     3361 ccctttttca tgttcaccaa catcggcagt cccgtatagg ggtcatagaa attttgccct
     3421 gctttcgcga tccgtgtgag gagatctcca tcgagggaac cacgtctgag ccaggcttgc
     3481 aagctggcaa gccggagaga ggcatccgta tcgaccacga atccgttgta ccggtcccat
     3541 ggagcaagcg gttcaagacc gacgatattc tcgatcggat cggtgaaata atccgtcaga
     3601 ccggatgccg gaaagtggat atagtttttc cacttcggca acgaaccata tttcccttcg
     3661 ccggcagctt tgtacgacgc gtcgtaatag tcggcatagt cgttgagacg gcgttgcatc
     3721 ggcaaaggga gagccgatgc caccatcgta tacaccggct gttcttcggc tcgcgctgct
     3781 ttcagctgag cgtcgtacat ctttgaagcg acgactagtt cactctgcat gggccagcgc
     3841 atcgagagct ccccctgatc aagcgggcga aggaatttgg agatgcgtcc caggtatttt
     3901 ccgtcgaagt cagaacggac gagtaacccc gaggcgaccg ctatgtcgtc attgatcgcc
     3961 t
//
LOCUS       DATNDD010000255         3700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3616443, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000255 DATNDD010000000
VERSION     DATNDD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616443"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VN647_06155"
     CDS             <1..764
                     /locus_tag="VN647_06155"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWG96648.1"
                     /translation="IIHLNERDCSIQRRHQKLIEIAPSLILTQALREEMGNAAIAIAR
                     AVNYDNAGTVEFLLDQEGKFYFIEMNPRLQVEHTVTEQITAIDIVRNQIKIAAGLPLN
                     IQQKDVILQGHAIQCRINAEDPKNNFLPCTGTVTAYLSPGGIGVRIDGAVYKDYTIPP
                     YYDALLAKLTVRGRTWEEAVSRMRRSLEEYVLRGVKTTIPFMEAIMQERDFIAGRFDT
                     SYLDTHPELYSYHEFEQPEDLVLALSAAIAAYEGL"
     gene            808..2757
                     /gene="oadA"
                     /locus_tag="VN647_06160"
     CDS             808..2757
                     /gene="oadA"
                     /locus_tag="VN647_06160"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HWG96649.1"
                     /translation="MAKRRPKKPQKKTRSSAPTIKTLKTVAARPSEFTITPAAGKPVL
                     ITDVALRDGHQSLLATRMRTEDMLPIAQKLDTVGYWSLEVWGGATFDTCLRFLKEDPW
                     ERLRALRAAMPNTKLQMLLRGQNLVGYRHYADDVVERFIERSATNGIDVFRIFDALND
                     VRNLDRAVSEVKACGKHAEATICYTVSPVHSIDRFVDLARKLEDLGTDTICIKDMAGL
                     LAPLDAYHLVRRLKAAVKVPLHLHSHYTSGMASMASLMAILGGLDMLDTSISPLAGGT
                     SHPATETLVASLQNTPYDTGLELASFQPITEHFRMVRRKYRQFESDFTGVDAEILMSQ
                     IPGGMLSNLAAQLTEQNALDRMKEVLDEVPRVRKEMGYPPLVTPTSQIVGTQATLNVL
                     TGERYKVITTETKNYFLGLYGRAPGQVDLDVMVRATGDDTPIKTRPADRLEPELDEAK
                     KELPDSAQTIEDQLSFVLFPSIARDFFEAREKGELTPEPLELGSAKGPATAHELHLAP
                     VEFNVIVHGETYHVKVSGSGRKIDGRKPYYIRVNDKLEEVSLEPIQEVLAGVPESQEP
                     QSGGKPKRPRPSKPGDVAPPMPGRVVKILVAVDDRVKVGDPLLIIEAMKMESQVPAPI
                     DGRVTVILAAEGDNVKTDETVIQLE"
     gene            2732..>3700
                     /locus_tag="VN647_06165"
     CDS             2732..>3700
                     /locus_tag="VN647_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG96650.1"
                     /translation="MKRSFNWSRFSADSRSRSCSPRHLVPLILCLVSYLMTACAQPLQ
                     IPPYFETLGHTPIERIPIKTVLVHDQRIAYLDVGNGPPVILIHGFGGSMWQWEHQQHA
                     LSQHFRTLTLDLLGSGLSDKPDIEYLPNQMLDFCIGFMDALQIEKATLVGNSLGAGLV
                     IGMTLTHPTRVDKLVLIDGLPSHVLAKLTSRSFRQVLESRAPSWLVSFGNWLFGGLVT
                     ESVLKEIVHDHNLLTPAVIERSNRNRRRPGIIKPIMAVRNALPSWETDFAPHLSAITH
                     PTMIIWGNDDRVFPLAVGEELHHRIRGSQFVKIPDAGHMPQWERPDL"
BASE COUNT          843 a         1090 c          990 g          777 t
ORIGIN      
        1 atatcatcca tttgaatgaa cgtgattgtt cgatccagag acgccaccag aagttgattg
       61 agatcgcgcc gtccttgatt ctgacgcagg cgctccggga agagatgggc aacgccgcca
      121 tcgccatcgc ccgcgcggtg aactacgaca acgcgggaac cgtggagttt ctgctcgatc
      181 aagaaggcaa attctatttc atcgagatga atccgcgcct ccaagtcgaa cacacggtga
      241 cggaacaaat cacggccatc gatatcgtgc gaaatcagat taaaatcgcg gccggcctgc
      301 cgctcaacat tcagcaaaag gacgtcatcc tccaaggtca cgccatccaa tgccggatca
      361 atgcagagga cccgaaaaac aatttcctgc catgcacggg caccgtcacg gcctatctct
      421 ctcccggtgg aatcggtgtt cgcatcgacg gtgccgtcta taaagattac acgatcccgc
      481 cgtactatga tgcgctgctg gccaaattga cggtccgggg tcgcacctgg gaagaagccg
      541 tcagccgtat gcgccgctct ctcgaagagt acgtgctccg tggagtgaaa accactattc
      601 ccttcatgga ggcgattatg caggaacggg actttattgc gggacgcttc gacacgtcgt
      661 acctggatac acaccctgag ttgtactcgt accacgagtt tgagcagcct gaggatctgg
      721 tgttggcgct gtccgccgca attgccgcct acgaaggtct ctaatccgaa caaatcactt
      781 tgcttccaaa gagatgcgct gaacatcatg gcgaaaagac gaccaaagaa accgcagaaa
      841 aaaaccagat cgtccgctcc gacgatcaag actttgaaaa ccgtcgcggc ccgccccagc
      901 gagttcacga tcacgccggc tgccggcaaa ccggtcctga tcacggatgt cgcgctccga
      961 gacggccatc aatcattatt ggcgacgcgc atgcgcacgg aagacatgct gcccatcgcg
     1021 caaaagctcg acacggtcgg ctattggtcg ctggaagtat ggggcggcgc caccttcgac
     1081 acctgtttgc gttttttaaa agaagatcct tgggaacggc tccgggcctt gcgggccgcc
     1141 atgcccaata ccaagctcca aatgctgttg cgcgggcaaa acctcgtcgg ctatcgccat
     1201 tatgccgacg acgtggtgga acggttcatc gaacgttcgg ctactaacgg catcgatgtc
     1261 ttccgcatct tcgacgcgct caacgacgtg cggaatctcg atcgggccgt gagcgaagtg
     1321 aaagcttgcg gcaaacatgc tgaagccacc atctgttaca ccgtcagccc ggtccatagc
     1381 atcgatcgtt ttgtggactt agccaggaag ttggaagatc tgggaacaga tacgatttgt
     1441 atcaaggaca tggccggact tttggcgccg cttgacgcat accatttggt gcgtcggttg
     1501 aaggccgccg tcaaggtccc ccttcatctc cactcacatt acacgtccgg tatggcttcc
     1561 atggcttcgt tgatggcgat ccttggaggc cttgacatgc tcgatacctc gatctctccg
     1621 ctggcaggag gaacttccca tccggccacg gaaacgttgg tcgcttcgct ccagaatacg
     1681 ccctacgata cgggattgga gctcgcctcc ttccagccga tcaccgagca cttccgaatg
     1741 gttcgccgca aatatcgcca attcgagagc gactttaccg gcgtggatgc ggaaatcctc
     1801 atgtcacaga ttcccggcgg catgttatcg aacctcgccg cccaactgac ggagcaaaat
     1861 gcgttggacc ggatgaagga ggttctcgac gaagtgccgc gtgtgcggaa agaaatgggt
     1921 tatcccccgc tcgtcacccc gaccagccaa atcgtcggaa cacaggcgac cctcaatgtg
     1981 ctgaccggcg agcgatacaa agtcatcacc accgaaacca agaactattt cttgggattg
     2041 tacggccgtg ctcccggaca ggtcgacctc gacgtgatgg tacgcgcgac cggtgatgac
     2101 acgccgatca aaaccaggcc ggctgatcga cttgagccgg aattggatga ggcgaaaaaa
     2161 gagcttcccg attcggctca gaccatcgag gaccagctgt cgttcgtgtt gtttcccagc
     2221 atcgcacgag actttttcga agcccgtgag aaaggcgagt tgacccctga gccgctcgaa
     2281 ttgggctctg cgaaagggcc tgccaccgct cacgaactgc acctggctcc cgtggaattc
     2341 aatgtgatag tgcacggcga aacctatcat gtaaaggtct cgggctctgg tcgaaagatc
     2401 gacggacgta agccctacta tatccgtgtg aatgataagc tggaagaagt gtcgctcgaa
     2461 cccatccagg aagtgctcgc cggtgtcccg gagtctcagg agcctcaatc gggaggaaaa
     2521 cccaaacgtc ccaggccttc gaaaccgggc gatgtggctc cacccatgcc gggtcgagtg
     2581 gtgaagatcc tggttgcggt cgacgatcgg gtcaaggtcg gcgatcctct gttgatcatc
     2641 gaagcgatga agatggaaag ccaagtgccg gcgccgattg atgggagagt caccgtgatc
     2701 ctggctgcag agggtgacaa tgtgaagacg gatgaaacgg tcattcaact ggagtaggtt
     2761 cagcgccgat tcgcgctctc gctcatgcag tccccggcat ctggtgccgc tcatcctgtg
     2821 tcttgtatcg tacctcatga ccgcctgtgc ccagcccctg cagatcccgc cctattttga
     2881 aacgttagga cataccccga tagaacgtat cccgatcaaa accgtcctcg ttcatgatca
     2941 gcggatcgcg tatctcgatg tcggcaacgg cccgccggtc atcttgatcc atggcttcgg
     3001 tggatccatg tggcaatggg aacatcagca acatgccttg tctcaacatt tccgcaccct
     3061 gacgctcgac ctactcggtt ccggattatc cgacaaaccg gacattgagt acctgccgaa
     3121 tcagatgttg gacttctgta tcggcttcat ggatgcgctt cagattgaga aagccacact
     3181 ggtcggcaac tccctgggtg cggggttggt cataggaatg acattgaccc atccgacccg
     3241 cgtcgacaaa ctcgtactca tcgacgggtt accgtctcat gtcctggcca aactcaccag
     3301 ccgatcattc aggcaagtct tggaatctcg agccccttcc tggctggtct ccttcggcaa
     3361 ctggctattc ggcgggctgg tcactgagtc cgtcctgaag gaaatcgttc acgaccacaa
     3421 ccttctcacg ccggctgtta ttgaacggtc caaccgtaat cgccgacgtc ccggtatcat
     3481 caaacctatc atggccgtga ggaacgctct cccctcttgg gaaaccgatt ttgctccaca
     3541 cctcagcgct atcactcacc ccaccatgat catctggggt aacgacgacc gggtgttccc
     3601 cttggcagtg ggtgaagaac ttcaccatag gatccgtgga tcacagttcg tcaaaattcc
     3661 cgatgccggc catatgccgc aatgggaacg cccggacctg
//
LOCUS       DATNDD010000256         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3465162, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000256 DATNDD010000000
VERSION     DATNDD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465162"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VN647_06170"
     CDS             <1..658
                     /locus_tag="VN647_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG96651.1"
                     /translation="GRESGMYSARILLADDHPLVLESVKQLLEPDFSVVGTAQTGQQV
                     LEQAQKLNPDIVMLDANMPGMNGFEAARKLKTILPAVKIIFVTMLTEAIAVSEGFRAG
                     AMGYVLKQDASDELHAAVKSVMANRRFVSSKLDMEVREAMECQWSRPEGYTTNLTERQ
                     RQILAMLANGSSTKNIAKELNISMKTVEFHKANITRKLGVRTTSDLIRVALASGMTAL
                     "
     gene            complement(704..1357)
                     /locus_tag="VN647_06175"
     CDS             complement(704..1357)
                     /locus_tag="VN647_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG96652.1"
                     /translation="MNAQPTILLADDHVLVAQGIQKLLEPEFELLRIVPDGRALLTAV
                     EECSPDVVIVDISLPLLNGLDASRQILKQNPAIKVIVLTMHSEQSFVTEALRIGVSGY
                     VLKQSVGSELLQALKEVLSGHTFISPIVADQGVRPHPDKDTEEAPKGFAHTLSLRQRE
                     VLQLVAEGKSIKEVAAVLNVSIKTVEFHKTRIMRQLGMRSAAQLTKYAIANGLIAIH"
     gene            complement(1545..1745)
                     /locus_tag="VN647_06180"
     CDS             complement(1545..1745)
                     /locus_tag="VN647_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96653.1"
                     /translation="MLEEYSAAEKLVESVDEWEVKGAEDQAMEVGEAETGRDQEARGQ
                     DQGLDPAYQNIAPCLSSYNQSS"
     gene            complement(2102..>3255)
                     /locus_tag="VN647_06185"
     CDS             complement(2102..>3255)
                     /locus_tag="VN647_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96654.1"
                     /translation="LRALTNELNLTEQRERKRLAGDLHDYLAQLLIVMRMKLRQAIPL
                     VPTEKATALLKDADQALTQALDYTRSLVSELAPPTPDEFELLQSLEWLASQMRQHGLT
                     VEIQPAAETLSLPEDQAILLFQSVRELLSNVLKHAHTDEATVSLMITSNSELHVTVED
                     RGNGFDAAALELSSADSTRFGLFSLKERMATMRGRVSIDSALNRGTKVSLIMPYWPVQ
                     PRDESTEHSVPNAESSSPCLESTTSLIHPKLSVQDLALHEKPRIRVLLADDHAIVRQG
                     LRSILESYPDIDIIGEVADGKESLDSVERVRPSVIVMDLNMPRMNGIDATAIIKSRYP
                     EIIVLGLSVNAGDDNQAAMIQAGASALLPKEAAVDQLYEMIHDAVATRS"
BASE COUNT          739 a          873 c          856 g          787 t
ORIGIN      
        1 aggcagagaa tcgggtatgt atagcgcaag aattttgctc gctgacgacc atccgctagt
       61 tctggagagc gtcaagcaat tgctcgagcc ggacttttcg gtcgtaggga ccgcacagac
      121 ggggcagcag gtgctggaac aagctcagaa gttgaacccg gatatcgtga tgcttgatgc
      181 caacatgccg gggatgaacg gatttgaagc ggctcgaaag ctgaaaacca ttttgcctgc
      241 ggtcaaaatc atctttgtca caatgttgac tgaagcgatt gcagtcagcg aggggttccg
      301 tgcgggagcg atgggctatg tattgaagca ggatgcttcg gacgaactcc atgccgcggt
      361 caagagcgtc atggcgaatc gacgatttgt atcttccaag ctcgatatgg aagttcgtga
      421 agcgatggaa tgtcaatggt cgcgaccgga aggctatacg accaacttga ccgagcgaca
      481 gcgtcaaatc cttgcgatgc ttgccaacgg atcttccacc aagaatattg ctaaggaact
      541 caacatttca atgaagaccg tagaatttca caaagccaac attacccgca agctcggtgt
      601 ccgtaccacg tccgatttga ttcgcgttgc actcgcatca ggtatgacgg ctctgtaact
      661 tgtaagtaga cacccaccga ccagttcggc atatcgagcc gggctaatga atggcgatga
      721 gtccattcgc gatcgcatac ttcgtcagct gagccgctga ccgcatgccg agctgcctca
      781 taatccttgt tttatgaaac tcgacggttt tgatcgatac gttcaagacg gccgccactt
      841 ctttgatgga ttttccttca gccacgagtt gcagcacctc ccgctgtcgc aagctgagtg
      901 tgtgagcgaa tcctttggga gcttcttcgg tgtccttatc agggtgggga cgtaccccct
      961 ggtcggcaac aatgggagaa atgaacgtgt gaccgctcaa gacttcttta agagcctgca
     1021 acaattctga tcctacggac tgcttcagta catagcctga tacgcctatg cggagtgctt
     1081 ccgtcacgaa actctgctcc gagtgcatcg taaggacgat gactttgata gccgggttct
     1141 gcttcaaaat ctgacgggat gcatccaatc cgttgagcag cgggagagaa atatcgacga
     1201 tgaccacatc cggtgaacat tcttcgacag ccgtgagtaa ggcgcggccg tccggaacga
     1261 tcctcagcag ttcgaattcc ggttccaaca gtttttgaat cccctgggcg acaagaacat
     1321 gatcatccgc caataagatg gtaggttgag cattcatggg cgaacctcct cctcggctta
     1381 tcccaaatcg aacggacaac ccttcctaca acttgaaaca tgctcattac ccatctgcat
     1441 cagttcgggc tgccgtcatg ccttcaaaag cggattgagt gcgaactgtt ctcaaggggg
     1501 cccactagtc cactgagcag ggggccattg ctatactgct gtcatcatga tgattgattg
     1561 taagaggata aacaaggggc aatattttga taagccgggt ccagaccctg atcctgaccc
     1621 cttgcctcct gatcccgacc cgtttccgcc tccccaacct ccattgcctg atcctcagcc
     1681 cccttcacct cccattcgtc caccgattcc acaagttttt ccgctgcgga atattcctcc
     1741 aacatggatg gaagccgaat ccgccattca tgtggtcagt cgagtcagaa ggcgagaaga
     1801 gcatccttct cacataaagg ggatacatca tgcgttattt catcctctgg ctattgggtg
     1861 tgccggtgag cctgcttgtt ctcttgtatt ttttggggat tctataagaa catcgtcgcg
     1921 tcataagctt ctgcaccgac aaaataaata ccgtctccct ctctatccaa ctactaggta
     1981 tgcccctagc ccacatcatt catgggcact tccgctgcaa ttgtggcggc atgttggcgg
     2041 gcggactcgc ccgctcggat ggtactcgtc tgcatcgccc cataaatcac ttacaagcga
     2101 gtcatgatct tgtcgcgacg gcgtcatgaa tcatttcgta caattgatcg actgcggctt
     2161 ccttcggcaa cagggcggac gcgccggctt gaatcatagc cgcttgatta tcatcaccgg
     2221 cattcacaga caaccccagg acgatgattt cggggtagcg ggatttgatg attgccgtag
     2281 catcaatgcc gttcatcctc ggcatattga gatccatgac gatgactgaa ggccgcacgc
     2341 gttcgacgga gtcgagcgat tcttttccat cggcgacttc acctatgatg tcgatatcgg
     2401 gatagctttc cagaatgctg cgcaatcctt gtcgcacgat cgcgtgatcg tccgccaata
     2461 agactcgaat gcgcggtttc tcgtggagtg ccaagtcttg aacgctgagt ttcggatgga
     2521 tgagagatgt tgtggattcg agacaaggac tcgatgactc ggcgttcggc acggaatgtt
     2581 cggtgctttc gtccctcggc tgtaccggcc aatagggcat gattaaagag acctttgtgc
     2641 ctcgattgag agcggagtcg atggaaactc gccccctcat ggttgccata cgttccttca
     2701 ggctaaagag accgaagcgg gtcgagtcgg cactggacag ctccagtgcc gcagcgtcga
     2761 atccgttgcc cctgtcctcc accgtgacgt gcagctcact gttcgaggtg atcatcaaag
     2821 acaccgtcgc ctcgtccgtg tgggcgtgtt tgagcacgtt ggagaggagt tcgcgcacgg
     2881 attgaaacag caagatagcc tgatcttccg gcaaggagag cgtttcggcg gcaggttgta
     2941 tctccactgt aaggccatgt tgccgcatct gagacgccaa ccattccaac gactgcaaga
     3001 gctcgaattc atccggcgtc ggcggagcca attcgctgac taatgatcgg gtgtagtcaa
     3061 gagcttgtgt gagcgcttga tcggcgtcct tcagaagagc tgtcgctttc tcagtcggca
     3121 caagcggtat cgcttgacgg agcttcatgc gcatgacgat gagcagttgt gccaaataat
     3181 catgcagatc tcctgcgagt cgtttccgct cgcgttgctc agtcaggttg agctcgttcg
     3241 tcagcgcccg caacc
//
LOCUS       DATNDD010000257         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2457704, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000257 DATNDD010000000
VERSION     DATNDD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457704"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VN647_06190"
     CDS             <1..311
                     /locus_tag="VN647_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWG96655.1"
                     /translation="IVVFQRHATELFQLAPTERFQLIEEVNRVAQTLADVYHAKKMNY
                     ELLGNQLPHIHWHVIPRLPGDPVPLEPVWRIPHSPLLLTGAALHEAIDLLAHRLRMTL
                     "
     gene            346..1611
                     /locus_tag="VN647_06195"
     CDS             346..1611
                     /locus_tag="VN647_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96656.1"
                     /translation="MKNYRGSPSRRESLLMHPRSRLLALTVAATVYASFLASSHSSGE
                     DNLSDNGDSPQVSESTSGLGFNEPTDAVTEIASLRRTIRLLPNDPDYRLKLAGALSRV
                     GDPDAAIEECRAAIKLQPDDGNAHLQLGLILMAKQEWQAAASALGEAIRLDPNLSHAH
                     YSLGSVYYSLGNVTAALQSYRRTLDLHPHFPDAHYRLALLLRIAGRTREAVQHLETAA
                     AGGVSQARLFLGNAYQTGQGVEKNLGLAIFWWMQAADLGQQTAADSLSKMRRQALSSE
                     STKQRRIELQSAFQIYRNTLWNNFPDISRPTQQQSLGKVLLEQNPVEDAVPVLLKECF
                     ALSEEAHAELAALYETGWERSLKPYDKNILACFESTSADGFAPAKKILARIYAKGLGV
                     DADIPKAKTLLKGLPKQETQSLIEELRLP"
     gene            1616..>2629
                     /locus_tag="VN647_06200"
     CDS             1616..>2629
                     /locus_tag="VN647_06200"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWG96657.1"
                     /translation="MPDWQKPWRLFIASLPLQALGIGMLATLLSTTLWSAAPATLTAL
                     EYTVYDTWSRHRAAARISPSLTIVARDAASEKRFGKGPWDRSILAQLIVTIHEAGAAV
                     IGVDHRLDYASPAQLGGAASDALFLETLRAVSPIVLIHNIDSLLQSDAAIYGHITVAS
                     DLDHVSRHIPSSIRIGTRTVPAFGSVLSEVFHRRNAVPVRFSSGNSDADVLISIVGDG
                     SLAALQPIPLSSVWNTVERHDEPQLASWFKDKVVVIRASPGGSETWLLPSGSMVDGIT
                     AHLHFLNALLNHERIDSFSWFGRFTVTLVLASLAAWSLLRFRGLMGLLLAGAVVVLYG
                     TLTV"
BASE COUNT          534 a          816 c          691 g          588 t
ORIGIN      
        1 ccatcgtggt cttccagcgc catgccactg agttattcca gcttgcaccc accgaacgct
       61 ttcaactgat cgaggaagtc aatcgagtag cccagacttt agccgacgtc tatcatgcga
      121 agaagatgaa ctacgaactc ctcgggaacc aactccctca tatccattgg cacgtgatcc
      181 cacgcctccc cggcgatccg gtcccgctgg aaccggtctg gcggataccg cacagtcccc
      241 ttcttctgac gggagcagcg cttcatgaag ccatcgacct cctcgcacac cgccttcgca
      301 tgactctatg aacccgatcg ttccgtattc cccgcggatg gtataatgaa aaactatcgt
      361 ggctctccgt cgaggcgtga aagtttgctg atgcatccca gatcgagact gttggccctc
      421 accgtcgccg cgactgttta cgcttcgttt ctcgcctcct ctcattcatc gggtgaggac
      481 aatctctctg acaacggtga ttcgccacaa gtgtccgaat caacttctgg gcttgggttc
      541 aacgaaccaa ccgatgcggt gacggagatc gcatcactgc gccgaacgat ccgtctctta
      601 ccaaatgacc cggattaccg tctaaaacta gccggagccc tttctcgtgt cggtgatcca
      661 gatgctgcca tcgaggaatg ccgggcggca attaaactgc aaccggatga tgggaacgct
      721 catctccagc tcggtctcat acttatggcg aaacaagaat ggcaggccgc cgcttccgct
      781 ctgggagagg ccatccggct ggatccgaat ctgtcccacg cccactacag cctggggagc
      841 gtatactatt cactcggcaa tgtaaccgcc gcccttcaat cttatcgacg gacacttgat
      901 ctgcatcctc atttccccga cgcacactat cgactcgcgc tgttattgag gattgcggga
      961 cgcacgcgag aagccgtgca acatctggaa acggcggcgg ccggtggggt gtctcaggca
     1021 cgattgttcc tcggcaatgc ctaccagacc gggcagggcg tggagaaaaa cctcggtttg
     1081 gcgatttttt ggtggatgca agcggccgac cttgggcaac aaaccgccgc tgattcgttg
     1141 tccaagatgc ggcgccaggc gctttcttcc gaatcgacga agcaacggcg catcgaattg
     1201 cagagcgctt ttcagatcta ccgcaacacc ctttggaaca actttcccga tatcagtcgc
     1261 ccgactcagc agcaatcgct ggggaaggtc cttctcgaac agaaccccgt ggaagatgcg
     1321 gttccggtac ttttgaagga atgcttcgcg ttgagtgaag aggcccatgc tgaactggcc
     1381 gccctctatg aaaccggctg ggaacggtct ctgaagccgt acgacaagaa cattctggca
     1441 tgttttgaat cgacctcggc cgacggcttc gccccggcaa agaaaattct tgctcgtatc
     1501 tatgccaagg ggctcggtgt cgacgccgac attccgaaag caaagactct cctcaaaggc
     1561 ctcccgaaac aagaaacaca atccttgatc gaagagctaa ggcttccata gttgtatgcc
     1621 ggactggcag aaaccctggc gcctgttcat cgcgtcgctt cctctgcaag cgctgggcat
     1681 cggcatgctg gccacactcc tctcgacgac gctgtggtct gctgcccccg ctactttgac
     1741 ggcgctggaa tataccgtct atgacacctg gtctcgccac cgtgctgccg cccggatcag
     1801 cccctcgctg accatcgtgg ctcgcgacgc ggccagtgag aaacgattcg gaaagggtcc
     1861 ttgggaccgc agcatcctcg ctcagttgat cgtgacgatc catgaggccg gtgccgccgt
     1921 catcggtgtt gaccaccgac tggactatgc cagccctgct cagcttggcg gagctgcaag
     1981 cgatgcgctc ttccttgaaa cgcttcgtgc ggtatcaccc attgtcctga ttcacaatat
     2041 cgattcactc cttcagtccg atgcagcgat ctatggacac atcacggtcg cgagcgatct
     2101 cgatcacgta tcccgccaca ttccgtcgtc catcaggata ggaactcgaa cggttccagc
     2161 ttttgggtcg gtcctgtccg aggtctttca ccgacgaaac gccgtgcccg tacggttctc
     2221 ttccggcaac tccgatgccg acgtactgat cagcatagtc ggcgatggat cgttggcagc
     2281 ccttcagccg atcccattgt catccgtctg gaacacagtc gaacgtcacg acgagccgca
     2341 gttggcttct tggttcaagg acaaagtcgt cgtgatccga gcaagtcccg gaggctcgga
     2401 gacatggctc ctaccgtccg gctcgatggt ggacggcata accgcccacc tccatttcct
     2461 gaatgcctta ctcaaccatg aacggatcga ctcgttcagc tggttcggtc gcttcaccgt
     2521 taccctggtg ctggcctcgc tggctgcttg gagcctctta cggtttcgcg ggttgatggg
     2581 gctcttgctc gccggtgccg tcgttgtttt gtatgggacg ctgaccgtc
//
LOCUS       DATNDD010000258         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2872813, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000258 DATNDD010000000
VERSION     DATNDD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872813"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VN647_06205"
     CDS             <1..549
                     /locus_tag="VN647_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96658.1"
                     /translation="KPEPFCYHPDLLRPLGRFLDGLAARAVISRDALIALYHHVYGWG
                     PGEVIAVTGLNGLESQRIYKNFRRWRENGWQRTMDEMGLTKVELAELGNQQQRHRQRF
                     NSEAERLIRVAHAHYRKSEPDHYPCLSRSQWGEMFTQGYGCDYRIWHLALCLDCMQTA
                     WGLGSNGSAVGEKPRLELQVRP"
     gene            616..1863
                     /locus_tag="VN647_06210"
     CDS             616..1863
                     /locus_tag="VN647_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96659.1"
                     /translation="MGQEEDLELYRSRLLESLNGQASRCVQGVSDPIQVYVNERRVGE
                     LEGAGIGAMLQVRCIDHIDTVQLRTEEGILLGGLTAPEHGFRTDRLRLSADTIELCVH
                     NTAQGGTLNALFMPAPGFWSRAWKSIAEAADRLVARRPDTAFAYGMRMVAFTQALLAI
                     AVVGLVADRVTTWMTPERTPSAVVQTASLPASPPAETARLEQQLDEVARIQAKIGEAL
                     ATQQNGIVQLHQAMAKLSSTQESVASSMVMVKEEVKEELEKRLEAVGHEADRPTRHVA
                     SKGRVEQEQLEAAIHSLTADNDRLSKEVAGLEENNQSLKAKLQTAVANVTKAADPSPE
                     RLLKMADSMQGNQQPFLFWVTFSDGTSQESIDQWVNGMKGHKGAVNEGWQEVRIVPPP
                     VPPDRFLEQIRGEKIVKAARISQ"
BASE COUNT          505 a          518 c          656 g          445 t
ORIGIN      
        1 aagccggaac cgttttgtta tcatccggat ttgctgagac cgctcggccg atttctcgac
       61 ggacttgcag ctcgggcggt catcagccgt gatgcgctga tcgcgctcta tcatcatgtt
      121 tatggatggg gccctgggga agtgatcgca gtgaccggac tcaatggatt ggagagccag
      181 cgcatctata aaaattttcg acgctggcgt gaaaacggct ggcagcgcac gatggatgag
      241 atgggtctga cgaaggtaga gctcgcggag ctcgggaatc agcagcagcg tcatcgacaa
      301 cgatttaaca gcgaggctga gcggctcatc cgagtcgctc atgcccatta ccgaaaaagt
      361 gagccggacc attatccctg tctttcgcgg tctcaatggg gagagatgtt tactcaaggc
      421 tatggttgcg attacaggat ttggcacttg gcgctctgcc tcgattgtat gcaaaccgcc
      481 tggggcctgg gatccaacgg atcagctgtc ggcgagaagc cgcgtctgga attacaggtg
      541 cggccctaaa ggaccgagcc cgtatcgcgg atgtaacccg tgaacctgta atgaaagagg
      601 agttaaggag gatatatggg tcaggaagag gatctggaac tatatcgatc gcgactcctt
      661 gaatcgttga acggtcaagc cagtcgctgt gtccaaggag tatcggatcc cattcaggtc
      721 tacgtcaacg agcgtcgtgt cggggagctt gagggagcgg gaatcggcgc gatgctgcaa
      781 gttcggtgta tcgatcacat cgacacggtg cagcttcgca ccgaagaggg gattcttctg
      841 ggggggctca ctgctcccga gcatggattt agaacggacc gtctccggct ttccgctgat
      901 acgatcgagc tttgtgttca caacaccgca caaggtggaa cactcaatgc gctgttcatg
      961 cctgcgcccg gtttctggag tcgagcctgg aagagcatcg cagaagctgc cgacagattg
     1021 gtcgcccgcc gtccggatac agcgttcgca tatggaatgc ggatggtcgc cttcacgcaa
     1081 gcgctcttgg caattgccgt cgttgggttg gtcgccgacc gtgtgacgac gtggatgacg
     1141 ccggagcgta cgccctctgc ggtcgtccaa acggcgagtc ttcctgcatc tccgccggca
     1201 gaaacggcaa gacttgaaca gcagctggat gaggtggcac ggatacaggc gaagatagga
     1261 gaagcccttg cgactcaaca gaatggtatc gtgcagctcc atcaagccat ggccaaatta
     1321 tcatcgaccc aggaatctgt ggcatccagc atggtgatgg tcaaggaaga agtcaaggaa
     1381 gagcttgaga aacggctgga agcagttggt catgaggccg accgcccgac gcgacacgtc
     1441 gcgagcaaag ggcgggttga gcaggaacag cttgaagctg cgatccatag tctcaccgca
     1501 gataacgatc ggttgtcgaa ggaagtcgcg ggattagaag agaacaacca gagtctgaag
     1561 gccaaattgc agacagcggt ggcgaatgta acgaaagctg ccgatccaag cccggagcgt
     1621 ctcttgaaaa tggctgacag catgcagggc aaccagcagc ctttcctttt ctgggtgacg
     1681 ttcagcgatg gaaccagtca ggagagcatc gatcaatggg tcaatgggat gaagggccat
     1741 aagggagcgg tgaacgaagg ttggcaggaa gtacggattg ttcctccgcc ggtcccgccc
     1801 gatcgcttct tggagcagat cagaggagaa aaaatcgtaa aggctgcgcg gatcagtcag
     1861 taggagtatt cagtaggagt attatcgggg agagcattct tattccgcat cgtcgctcgt
     1921 acgcgatacg tgtcgacatc cacacgagca ggggttttcg gtgtattgca tccgggagag
     1981 ggacaagttg acgttcgttg acggcaaaat gttcggatta cgtcaaagga gctatgcgac
     2041 tgttcatttt tcagatgaat gccaatcatg aggctcaatg ttcggtcaat cgccctcgac
     2101 tggacatatt ccggcccgat cata
//
LOCUS       DATNDD010000259         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1198640, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000259 DATNDD010000000
VERSION     DATNDD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198640"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VN647_06215"
     CDS             complement(<1..466)
                     /locus_tag="VN647_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HWG96660.1"
                     /translation="MKKIILGFGVLALLVLPALGQDKEEDRVAKAGTVIKEILDIPDD
                     IPQDVIDRADCVVVLPSVLKFAFGIGGSYGRGVMTCRGGKNFHGPWSAPTLMALEGAS
                     FGLQLGGQATDFVLLLMSSKSASSILESKVKLGGDVSAAAGPVGRNASAETDV"
     gene            complement(680..>2155)
                     /locus_tag="VN647_06220"
     CDS             complement(680..>2155)
                     /locus_tag="VN647_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HWG96661.1"
                     /translation="SVSASFADVNNTGHEDLFVTTVNQGNVFFENDGHGNFKDVTEEA
                     GLGLVAHSSGSMFFDYDNDGLVDLLVCNVGVYTSNEKGHYGEYVGLKDAFLGHLYPER
                     FEYPVLYKNLGHYKFKDVTAEVGLRPYGWCGDATFADVNGDGWPDVFFLNMQGASHYF
                     ENVEGKKFVEKTQEYFPRTPWGSMGLKFFDYDNDGRMDLFVTDMHSDMMEEQAPHHEK
                     EKVQHHRPDKMLGGAADKFIFGNAFYHNLGAGQFEEISDRIGAEQLWPWGVSVGDVNA
                     DGWEDIFITAGMNFPFRYGINSMLLNDRGEKFLDSEFILGIEPRQQPYTPWFVMNCTH
                     HDMLPDLGLGQAVCGRRNDKFIVTVPKASRSSVIFDLDNDGDLDIVTNDFNSEPQVLI
                     SDLSQQRTIHWLKVVLIGTKSNRNGLGATVRLQINGQTYTKYNDGKSGYLSQSVQPLY
                     FGLGDATKIDRIEVDWPSGQKQVVSKGLDENQTLRITEAVQ"
BASE COUNT          494 a          604 c          532 g          525 t
ORIGIN      
        1 tgacatcagt ctctgccgag gcattccgac ccacaggtcc ggcagccgcc gagacgtccc
       61 cacccagctt caccttgctt tccaaaatac tggacgccga ttttgagctc atcagcaaca
      121 gcacgaagtc agtggcttgc ccgcccagct gcagtccgaa gctagcacct tcaagcgcca
      181 tcaaggtcgg agcactccac gggccatgga aattcttgcc gccacggcag gtcatcacac
      241 cccggccata gctgccacca attccgaagg cgaatttcaa gacggaaggt aaaacgacaa
      301 cgcaatccgc ccggtcaatt acgtcctgcg ggatgtcatc ggggatgtcc agaatttcct
      361 tgatgacggt tcccgctttc gctacccgat cctcttcctt gtcttgaccc agcgccggta
      421 acaccagcag agccaataca ccgaaaccta aaataatttt cttcatattg tttcctcaac
      481 tcggaatttt gctgttaatg gggagcttcc atgcggacag cactgatcgt ggccgaaagt
      541 gtgccgccat gataacaaac ttggcttctg gactcaccac acacttccgc acaagaatgc
      601 tactgcgttc cgcgggcgcg gtcgatctct caatacgaag tctaaagttt ggcggcggtg
      661 gcgcgatttt ccaggaggat tattggacgg cttcggtgat tctcaatgtc tggttctcat
      721 ccaaaccctt actaaccacc tgtttttgtc ccgacggcca atcgacctcg atgcgatcta
      781 tttttgtcgc gtcacccaga ccgaagtaga gaggctggac actctgggac aaatagccgg
      841 atttaccgtc gttgtacttg gtgtaggtct gaccgttgat ctgcaaccga acggtcgcac
      901 ccaggccatt gcggttggat tttgtgccga tgagaacgac tttgagccag tggattgtcc
      961 tttgttggga gaggtcgctg atcagcacct gaggttccga attgaaatca ttggtgacga
     1021 tgtccagatc gccgtcatta tcaagatcaa agatgacgga ggatcggctt gctttgggca
     1081 cggtgacaat aaatttatcg tttcgtctcc cgcagaccgc ctggcccaag cccaggtcag
     1141 gtaacatgtc gtgatgggtg cagttcatga caaaccaggg cgtgtagggc tgctgccgtg
     1201 gctcgatccc gagaataaat tcggaatcga gaaatttctc gccccggtcg ttcagcagca
     1261 ttgagttgat cccgtagcga aaagggaaat tcatgcccgc cgtgatgaaa atgtcctccc
     1321 acccgtccgc attgacgtcg ccaacgctga ctccccaggg ccacaactgt tccgctccga
     1381 tccgatcgga aatttcctca aactgacctg cacccaagtt gtgataaaag gcgttgccaa
     1441 agatgaactt atccgcagct ccacccagca tcttgtccgg ccggtgatgt tggacctttt
     1501 ccttttcatg atgtggggct tgttcttcca tcatgtcgga gtgcatgtcg gtaacgaaaa
     1561 gatccattcg gccatcgttg tcgtagtcga aaaacttcag tcccatgctt ccccagggtg
     1621 ttcgtggaaa atattcctgc gttttctcga caaacttctt gccttccacg ttttcgaagt
     1681 agtgactggc tccttgcatg ttcaagaaaa acacatccgg ccagccgtcc ccgtttacat
     1741 cggcaaaggt tgcatcgccg caccagccgt aaggcctgag accgacttca gccgtgacat
     1801 ctttgaattt gtagtgaccc agattcttgt agagaaccgg gtattcgaat cgttctggat
     1861 agagatgacc gagaaacgcg tctttgagcc cgacgtattc tccatagtgt cccttttcat
     1921 tgctggtgta cacgcccacg ttgcagacca gcaggtcaac caaaccgtcg ttatcgtaat
     1981 caaagaacat tgacccggaa gagtgagcca ccaagccaag gcccgcttct tcagtaacgt
     2041 ctttaaagtt gccgtggccg tcgttttcga agaagacatt cccctgattg acggtggtca
     2101 cgaagaggtc ttcgtgaccc gtattgttga catcggcaaa ggaggcactg acgct
//
LOCUS       DATNDD010000260         4072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3049174, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000260 DATNDD010000000
VERSION     DATNDD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049174"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1066)
                     /locus_tag="VN647_06225"
     CDS             complement(206..1066)
                     /locus_tag="VN647_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWG96662.1"
                     /translation="MSLSLSRRNLLRGGAMLSASSLFYNHAFALAEESQPVSSSPIRL
                     GIASYTFRNFDQSHVIDFMKQLKTPYLNLKDVHLPMTPADEVAKHAAEYRDAGMILTA
                     AGTIYFPKDDDDDIRAKFEYCKAAGISLIVGAPTHQTLPRVEKFVKEYNIRLAIHNHG
                     PEDKQWPSPLTVLDAVKDMDKRIGCCIDVGHTMRTGTDVVSAIRKAGPRLFDIHMKDL
                     AKSDVKESQVAVGDGLMPVPHIFKALIEMNYPGQVDLEYEIFGSDPMPGVTKSFAYMR
                     GVLAGMGYKG"
     gene            1249..>4072
                     /locus_tag="VN647_06230"
     CDS             1249..>4072
                     /locus_tag="VN647_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWG96663.1"
                     /translation="MIRHKIVACVAIFLILSSANLMFGQAVNATLLGTISDSTGASVA
                     DANVTATEAKTSIAHESVSNASGNYTFPDLAPGTYTVTVEAKGFKKDTHQNISLLVNT
                     STRVDVSLQLGSISETVLVTTAPPVLQTDRADISTKIEAHQVASLPLGTNRNFQSLLN
                     LVPGTSPATFQHSQFFNAQSSLQTEANGNARMGNLYQIEGIDDDERTGLLQIIIPPAE
                     AIQSVDISTNNYEAELGRATGAVTNVILKSGSNAFHGSVFEFIQNNDVNARSYFGGPL
                     GHLSYNYFGGSIGGPILKDKLFFFGDYLRTTDHEAISNTFTIPPPTFYTPNASGFIDL
                     SAPLKSDGTGQVYDPTTGDGTVGNPRTPFANNQIPIGKVNPVSLAILTAIGKVAQPNR
                     NQSNLASPSNNFVTNLPFTKTTDSFDTKVDYALTGRDHFSGRYSYQRVLTFQAPAFGS
                     FLGGPAGGGFEGSGTQNSYSTGVNYDHVFSPTLFTEFRFGVAHLHNTSQPSDYGTDDA
                     TTIGIPGVNISGQPFTSGQAAVTIGNFTGPTMGYSASMPWIRAEANIDLVNNWTKVIR
                     NHTIKVGADLRRVRDDLLQDQTFSPRGAFTFAEAQTSEAGAKTNVANDIASFLLDVPS
                     QTGRDLNTFFPAYRQWWFFAFASDKWQATSKLTLDLGVRWEFYPPATPKIAGGFSNYD
                     PTTNNLVLAGIGGNPSNLGMQTRYRYFAPRTGFSYRATDNTVIRGGFGISYAPFPDNT
                     YAYNYPIRANNSYQPGGTSAFNPAVLSDGVTVATFQAGFPAPVPVVIPTNGIISASTP
                     ALTSQVYTVIPKNFKNPYVESWNVAVQQAFAGNLSMQLAYVANHGVNISSGQNINLPS
                     YYGGGNTSDPEYATFGRTAATNLFFKGFSSNYQSLQAQLNKRTSYGLSFTTAFTWGKG
                     LGYISGDDGGIIFYINQRRNYAPNDFD"
BASE COUNT          926 a         1182 c          989 g          975 t
ORIGIN      
        1 ttcgcgaacc atacagggtg acagtgccgg gccatggcgt tctctttttg cgtgtatcaa
       61 agtaaagccg aagcggtcgt cgaaatgtat ccaggttctc tcctcggatg cattccgccg
      121 accgcatttt gcaatttgcg aaccgcgcat caatatgtat ttccatcgat tgaaaagctg
      181 ctaagcccca aaataatata agagcttatc ctttgtagcc cataccggca agcacgcccc
      241 gcatgtaagc aaagctctta gtgactcccg gcattggatc tgacccgaag atttcgtact
      301 ccagatcgac ctgtccggga tagttcattt cgattagcgc cttgaagata tgtggtactg
      361 gcatcaatcc atcgccgacg gcgacctggc tctccttcac atcgctcttg gcgaggtcct
      421 tcatgtggat atcgaacagg cgcggccctg cctttcggat tgccgaaacc acgtcggtgc
      481 ccgtccgcat agtgtggcct acgtcgatac agcagccaat ccgtttgtcc atgtccttca
      541 cggcatcaag caccgtcaag ggagaaggcc actgtttgtc ctctggacca tggttgtgaa
      601 tggctaggcg aatgttatat tccttcacga atttttcgac gcgcggcaat gtctgatggg
      661 tcggcgcacc cacgatcagc gatattcctg cagccttgca atactcgaat tttgctcgga
      721 tatcgtcatc gtcgtccttt ggaaaataga ttgtgcctgc tgcggtcaaa atcattccag
      781 cgtctctgta ctccgctgcg tgcttggcca cctcgtccgc aggagtcatg ggaaggtgca
      841 cgtctttcag gttcagatac ggagttttga gttgcttcat gaaatcgatc acatgagatt
      901 ggtcaaaatt tctgaaggtg taactggcga ttccgagacg tatcggagac gaggatactg
      961 gctgactctc ttccgcaaga gcgaaagcat ggttataaaa aagagacgac gcgcttagca
     1021 tcgcgcctcc acgcaacagg ttacgacggg acaatgaaag gctcatgcag ggcatcctaa
     1081 ctctgctgac gtaagctcgt ccagcctcgt tttcaggtgc aattaggcgg ccatcaccta
     1141 tcagttattt tgtattgcta ttttcaagct gtgctgatac tgtttcgttc gttcttcaaa
     1201 caataatttg gttgcctttg tactaatgca agactcctgg aggttcacat gattcgccat
     1261 aagatagttg cctgtgtcgc catctttctc atcctgagct ctgcaaacct gatgttcggg
     1321 caggccgtca atgcgaccct gcttggcaca atcagtgata gcactggcgc atccgtcgcc
     1381 gacgcaaatg tcactgctac cgaagcaaag acgagcatcg cgcacgagtc tgtctccaac
     1441 gccagcggca actatacgtt tcccgatctc gcgcccggca cctataccgt gacggttgag
     1501 gccaagggct tcaagaagga tacccaccaa aacatctcgc tgttggtcaa tacgtcgacc
     1561 cgtgttgatg tgagccttca actcggaagt atctccgaga ccgtactggt aacaacggca
     1621 ccgccggtac ttcagacaga ccgtgctgac atcagcacaa agatcgaggc gcatcaggta
     1681 gccagtctgc cgctgggcac aaaccgcaac tttcaatccc tgctaaatct tgttcctggt
     1741 acatcgccgg caacctttca acactcgcag ttcttcaatg cgcagagttc tcttcagact
     1801 gaggcgaacg gaaatgcgcg catgggaaat ctttatcaga tcgagggcat cgacgacgac
     1861 gagcgcaccg gtctgctaca gatcatcatc ccgcccgccg aagcgatcca atccgtcgat
     1921 atctcgacga acaattacga agctgagcta ggcagagcca ccggcgccgt gacaaacgtc
     1981 atcttaaagt cggggagcaa cgctttccat ggctctgtct tcgagttcat tcagaacaat
     2041 gacgtcaatg cgcgctcgta tttcggcggt cccctcgggc atctttccta caattacttt
     2101 ggcggcagta ttggcggtcc catcctcaag gacaagctgt ttttcttcgg agactatctc
     2161 cgcaccacgg accacgaagc gatctccaat acgttcacta ttcctcctcc cacgttctat
     2221 acgccgaacg cttccggttt catcgatcta agcgcaccgc ttaaatcaga cggaaccggg
     2281 caggtctacg atcccactac tggcgatgga acagtgggaa acccgcgtac cccttttgca
     2341 aacaatcaga ttcccatcgg aaaggtaaat cctgtctccc tggcgatcct gaccgcaatc
     2401 gggaaggtag cgcagccgaa ccggaaccag tcgaatcttg cttccccatc caataacttc
     2461 gttacaaatc ttcccttcac caaaacaacg gatagctttg ataccaaggt ggactatgcc
     2521 ctgactggcc gagaccactt cagcgggcga tatagctatc agcgcgttct taccttccag
     2581 gcgcctgcct ttgggtcttt tttaggtggc cccgctggtg gaggctttga aggtagcggc
     2641 actcagaact cctatagcac cggcgtgaac tacgatcacg tgttctcgcc taccctgttc
     2701 acagagtttc gattcggggt tgcgcatctt cacaacacgt cgcagcctag tgactatgga
     2761 actgacgatg cgacaaccat tggtatcccc ggcgtcaaca tcagcggtca gccgtttacg
     2821 tctgggcagg ctgctgtgac catcggcaac ttcaccggtc cgaccatggg ttactctgcg
     2881 tcgatgccat ggatcagggc cgaggccaat attgacctcg tcaacaactg gaccaaggtt
     2941 atccgcaacc atacgatcaa ggtgggtgcc gatcttcgcc gtgtacggga cgatcttctg
     3001 caggatcaga ccttcagccc gcgcggagcc tttaccttcg cagaggcgca gacatctgag
     3061 gctggcgcaa agactaacgt ggcgaacgac attgccagct ttcttctgga tgtgccaagc
     3121 cagactggac gggacctgaa taccttcttc cctgcatatc ggcagtggtg gttcttcgct
     3181 tttgccagtg acaagtggca ggcgacctcg aagttgacgc tcgatcttgg tgttcgctgg
     3241 gagttttatc ctcccgcgac gcccaagatt gcaggcggct tctccaacta cgatccgacg
     3301 accaacaatc ttgtgctggc cggtatcgga ggcaatccgt cgaacctcgg aatgcagacg
     3361 cgctatcgct acttcgcacc gcgcaccggt ttctcctatc gcgccaccga caataccgtt
     3421 attcgtggtg gcttcggcat cagctatgcc cctttccccg ataacaccta tgcctataac
     3481 tatcctatcc gcgccaacaa tagctaccag cctggtggaa cctcggcgtt caatccggcg
     3541 gtattgagcg atggggtcac ggtcgcgaca ttccaggcag gatttccggc gccagtgccc
     3601 gtcgtcattc ccaccaatgg aatcatctct gcaagtactc cagcgcttac cagccaggtc
     3661 tatacggtta ttcccaagaa cttcaaaaat ccctatgttg aatcatggaa cgttgctgtg
     3721 caacaggcat ttgcgggcaa tctctccatg caacttgcct atgtcgcaaa ccacggcgta
     3781 aacatctcga gcggtcagaa catcaatctt cccagctact atggtggcgg caacacctct
     3841 gaccccgagt atgctacttt cggtcggacg gcggccacga acctgttttt taaaggcttc
     3901 tcctccaact atcaatcgtt gcaggcccaa ctcaataagc gcacctccta tgggctgtca
     3961 tttacaacag cctttacctg gggcaaggga ttgggctata tcagcggtga tgacggcggc
     4021 atcatcttct acatcaacca gagaaggaac tacgccccca acgattttga cc
//
LOCUS       DATNDD010000261         4141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3636477, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000261 DATNDD010000000
VERSION     DATNDD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636477"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VN647_06235"
     CDS             <1..237
                     /locus_tag="VN647_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96664.1"
                     /translation="EAPEIIPFLGTSRLEEPINRYGATAVFHGHAHHGALEGRTRDGI
                     PVYNVALPLLRRAAPAQAPMRMLHVPIQQQVQAT"
     gene            249..1115
                     /locus_tag="VN647_06240"
     CDS             249..1115
                     /locus_tag="VN647_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96665.1"
                     /translation="MSGNQSTQNLYRLGLQDMLRSGVPFLVGGAFALENYTGVVRRTK
                     DLDVFALRKDVDGLMRFLAAAGYETEVRFPHWISKARKSGHVIDIIFGSGNGICAVDQ
                     KWFGAAPTATIMGERVRLVPAEEMIWSKAFVMERDRYDGADIAHLLHARSDRLDWGRL
                     LWRFDRHWRVLFSHLVLFGFVYPRRRLQIPHWLMRELIQRLGMEMQTLSSDRDICQGT
                     LLSWGQYLDHIECGEYKDARRHPRGSLTAGEIKFVTERFQEEQGGYQGTRTVAEDFVL
                     RDGPPRLSEPAA"
     gene            1112..1588
                     /locus_tag="VN647_06245"
     CDS             1112..1588
                     /locus_tag="VN647_06245"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWG96666.1"
                     /translation="MKTAEEKVTTMNDKAYVSLIGRLLIGLIFVMSGIHKIADPQGTQ
                     QYMAAMGITTGTTFLYAGAVFMEVVGGLSLWLGVWSKWGATALIIFMMPTTMIFHTNF
                     DDQNQTIHFLKNLAMMGGLLYVAVYGPGRLSIDRGVSRVNQDVESEVPSLKKVVHQ"
     gene            3211..3453
                     /locus_tag="VN647_06250"
     CDS             3211..3453
                     /locus_tag="VN647_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96667.1"
                     /translation="MNKEQFGQFWEQLKAPLKAKWSGISEKDLIEIKGNLDMFGTVLQ
                     RRYGELRKDEVAIWAHRRYCHWSGNYMGYKEETQAV"
     gene            3697..3861
                     /locus_tag="VN647_06255"
     CDS             3697..3861
                     /locus_tag="VN647_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96668.1"
                     /translation="MVEELGEAADEHAATLRIVTIPEDVKWVIVKTEGGEQVSEAHRT
                     WGGAKSHSWM"
     gene            complement(3951..>4141)
                     /locus_tag="VN647_06260"
     CDS             complement(3951..>4141)
                     /locus_tag="VN647_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWG96669.1"
                     /translation="GFVEMSTAEEAKAAITALNGSQMDGRSLTVNEAKPMEPRFGGAG
                     GRSGGSGRFGGGNSRNRY"
BASE COUNT          975 a         1045 c         1210 g          911 t
ORIGIN      
        1 gaagccccgg aaattatccc atttctgggg accagccgtt tggaggagcc catcaaccgc
       61 tatggtgcca cggcggtgtt tcacgggcat gcgcatcacg gtgccttgga gggacgaacc
      121 cgggacggca tccctgtgta taacgtcgcg ctgcctttgc ttcgtcgcgc ggcgccggcg
      181 caagcgccga tgcggatgct tcacgttccg attcaacaac aggtacaagc gacatgagcc
      241 ctcacgcgat gtcgggcaat caatccacgc agaatctgta tcggctcggg ctgcaagata
      301 tgctgcgctc cggagttccg tttctggtag gcggcgcgtt tgcattggaa aactatacgg
      361 gcgtggttcg acgcaccaag gatctcgatg ttttcgcgct ccggaaagac gtggacggtc
      421 tcatgcgatt ccttgccgct gcggggtatg agacggaggt ccggtttcct cattggatca
      481 gtaaagcgcg taaaagcggg catgtgatcg acatcatttt cgggtccggt aacgggattt
      541 gcgccgtcga tcagaaatgg ttcggcgcag caccgactgc gacgatcatg ggggaacgag
      601 tccgattggt gcccgccgaa gaaatgattt ggtccaaagc cttcgtcatg gaacgcgacc
      661 gttacgacgg ggcggacatc gcgcatttgt tgcatgcacg atccgaccgt ttggattggg
      721 ggcggttgtt gtggagattt gatcgccatt ggcgcgtgct gttcagccat ctcgttttgt
      781 tcggcttcgt ctacccgcgg cgccgattgc aaattcccca ctggctgatg cgtgaactca
      841 tacaacgact gggtatggaa atgcagaccc tgtcgtcgga tcgcgatatc tgtcagggaa
      901 cgctgctgtc ctggggacaa tatctcgacc acatcgaatg tggagagtac aaggatgccc
      961 gccgtcatcc acgcggatcg ctgacagccg gtgagatcaa atttgtgacc gagcggtttc
     1021 aagaggagca aggcggctat caaggaacca ggacggttgc agaagacttc gtgctgcgtg
     1081 atgggcctcc gaggttgtct gagcccgcgg catgaagacg gcggaagaaa aggtgacgac
     1141 gatgaatgat aaggcgtatg tcagtctcat cggacgatta ttgatcggtt tgatatttgt
     1201 gatgtcgggg atccacaaga ttgccgatcc ccaggggact cagcagtaca tggccgcgat
     1261 gggcatcacg acaggaacaa cgttccttta cgcgggagct gttttcatgg aagtggtcgg
     1321 tggattatcg ttgtggctgg gggtgtggtc aaaatggggt gcgacggcgc tcatcatctt
     1381 catgatgccg actacgatga tattccatac gaacttcgac gatcagaatc agaccatcca
     1441 ctttttgaag aacctcgcaa tgatgggtgg actgctgtat gtggccgtct acggacctgg
     1501 acgattgagc atcgatcggg gggtttcacg ggtgaatcag gatgtcgagt cggaggtacc
     1561 ctctctcaag aaagtggtgc accaataagg gacgcatcca cgctcgtcgg cgacgggatc
     1621 gaaccggtac gggctttttt gaatcaccgc ttccctcgcc gggccttacg ctcgatcagg
     1681 gctgtatcat acgcgcatcg gttttttatg gatagcccat accagggcgc cgatccatcc
     1741 gattaccgtc cagcctagta ctagattgag tgcgaaaatc gcaaaacggt tctcctgatt
     1801 tcgtgccgag gcgacaaagc tcgggatgaa gtagacgagc agggtccccg gtgtttgcag
     1861 actaaagttt ccaagatccg gcatagtcat tacacaacac gatggtccgt accggggaga
     1921 ctaaaacaaa tgtcgtgtca cgaaataact gtagacaagg acgctgatga ttcccgtcat
     1981 gtacgacacg gctgccgtag caagtggatg gtgaacgagc cattggacgg agttctgaag
     2041 aggattagga gacaccatca tgggtcaccg cctttctcac aaggtgacgg cggtgggcca
     2101 tccagaaccc gtcggcaaga tgtgggttga atcgtagacg ggttagggta tctcaatggc
     2161 aaaggatgtc catgctctca atccctaggg ctgcatcttt atattgcacg gcctaacggt
     2221 ctaatggaga cgaggaatct ataacgattc tccataagag tgaggttcgt agcaaagcta
     2281 ctcgcttgcc ccgctatttc cacagcgtat ctgcgatgcg aggaggtagg ttttcagttc
     2341 ttctgaataa tattgaacga tttttcgctc ttcaggagat aacggcttgc tgccaaagag
     2401 aaccgcagag gaaaggatca tttcgcagga acggatgaag ccatggattt ctttgcgtaa
     2461 cggagagaat ggactgctgg acgccggcat aggacaccgc ctttctcaca aggtgcggtc
     2521 tcggcacggt tcggtctggt cccgaagggt cgcctcgttt ttgtcggcga tcacctcact
     2581 cttagatggc ggcaccacct ccaaatggtt cttcaccgac ttgacccacc agccattcag
     2641 agaactccgg cctcggagcg gcagagactg aggaatcggt cacgctgaga agaagtagga
     2701 agcgtaagag acgggacagt caccgaggct tcacacctta caattctacc ttgggcgcaa
     2761 tgggtatcaa agtcaagcac tatatatgtc gatccggaga agtcaacggg agaaatcgaa
     2821 cgggaggcac gagagctgga ttcacggaga ggggctaagt accttggtcg cacctcgtga
     2881 aatagaaatg gactggttca aggcaatgcg ggacatttgt tttaagccat aacttgcatc
     2941 gctgatgatc ttcaatcact aaagccaacc gtccttacca ttaattacgc ttgactctct
     3001 tggtgaatgc gatcagagtc actctatgac gtttgagcta cactgactat cccatccctc
     3061 gcgccgttcc ttctcatgcc gttgttgcgc gtctgttttc ccggtctttg ccgcttctac
     3121 gcctgatctc cacaattagc tctctgccca gacgccggac gtgtcttggc cgaaaggagt
     3181 ggagacctca catctcacca aggagttcat atgaacaagg aacagttcgg acaattctgg
     3241 gagcagctca aggcgccgct caaggcaaag tggtcgggca tcagcgaaaa ggacctcatt
     3301 gagatcaaag ggaatctcga tatgttcggc acagtcctcc aaagacggta cggtgagctc
     3361 cggaaagatg aagtggcgat atgggctcac cggcgctact gtcactggtc tgggaactac
     3421 atgggctata aggaagagac ccaagcggtg tagattcgta gtcattcggc agtcgaggaa
     3481 cggcgcctac tactacaaag gaagcgaaca ggaagcgaac tgattaacag gagctatgac
     3541 gaatgccatc aagcgctgat ccggctgcgt gaattgggac aacgggaggc actggaagaa
     3601 gagattctgg agcctattgg cctgcggctg cggagcctcg ggaaccgagc ctgatatggg
     3661 aaacttattc cacgggatga tgagaacctc gttcgcgtgg tggaggaact tggagaagca
     3721 gccgatgagc atgccgcgac cctcaggatc gtgacaatcc ctgaggacgt gaaatgggtg
     3781 attgtcaaga ccgagggtgg cgagcaagtg agtgaagccc atcgcacatg gggaggagca
     3841 aagtcgcact cttggatgta agcgctatcc tgaaccaatc ggagaaacgg gaacccgaag
     3901 agatgcttaa ccaaagcacc ccttctggtt ccggtagaac gtggacgcgg ttaatagcgg
     3961 ttgcggctgt ttccaccacc gaaccgtcca ctcccacctg aacgaccgcc agcaccaccg
     4021 aaacgtggct ccatcggctt cgcctcattg acggtgagcg atcgtccatc catttgtgag
     4081 ccgtttaagg ccgtgatcgc cgccttggct tcctcggcgg tcgacatttc gacaaagcca
     4141 a
//
LOCUS       DATNDD010000262         4980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_159223, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000262 DATNDD010000000
VERSION     DATNDD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159223"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2129
                     /locus_tag="VN647_06265"
     CDS             <1..2129
                     /locus_tag="VN647_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HWG96670.1"
                     /translation="DFGGEIHYRIQGEHHNWNPETIYKLQHASRSNDPKTYAEFSQIV
                     NDESKQRSNLRGLLDFKFLPRPIPIEEVESAKEIVKRFNTGAMSFGSISKEAHETLAI
                     AMNRLGGKSNTGEGGEDPERFIPLPNGDSKNSYIKQVASARFGVTAHYLANARELQIK
                     MAQGAKPGEGGQLPGHKVDENIAKFRYATPGVQLISPPPHHDIYSIEDLAQLIFDLKN
                     SNPDAGVSVKLVAEVGVGTVAAGVAKAHADKVLISGDSGGTGASPLSSIKYAGIPWEL
                     GLAETHQTLVLNDLRGRIRVETDGQMKTGRDVVIATLLGAEEYGFATAPLIVEGCIMM
                     RKCHLNTCPVGIATQDPELRKKFNGKPEHIVNYLFFVAEEARQLMARLGFRTINEMVG
                     RVDKLKITRAVGHWKAKGLDLTPLLTIPDVSADVPRYCVQKQDHGLIDILDNKLVELC
                     KAAIERGEKVKLELPIRNINRTTGTVLSSKIAKKYGPDGLPEDTISIKFTGSAGQSFG
                     AFLAKGITLTLEGESNDYIGKGLSGGKIIVFPPKNILYNPEETILIGNTSLYGATQGE
                     AYFYGMAGERFAVRNSGAQAVVEGTGDHGCEYMTGGVVVVLGKTGRNFAAGMSGGTAF
                     VLDDAGTFQSRCNTGMVELEPVTTKDDKQLLHGLIAKHFMYTGSRKAKQVLDAFEAIL
                     PKFVKVMPVDYKRVLEERKRKASAVH"
     gene            2231..3664
                     /locus_tag="VN647_06270"
     CDS             2231..3664
                     /locus_tag="VN647_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWG96671.1"
                     /translation="MGDPKGFMKYAREGPKRKPIELRVLDWKEMYEPISEDKLKTQGA
                     RCMDCGVPFCQGHTGCPVVNLIPEWNDLVYRGRWKDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLGINEDPVSIRIIEWNIVDRGFNDGLVTPILPAVRTGKSVAIVGSGPAGLAA
                     AQQLARAGHDVTVFEKADRIGGLLRYGIPDFKMEKWVIDRRLEQMKAEGVKFQTGVMV
                     GEDITGEQLRQRFDAVGLTMGAEQARELPIPGRELKGVHLAMEYLTQQNKRTAGIPFS
                     DEPITAKGKRVVIIGGGDTGSDCLGTAHRQGCLEAHQFELLPEPPPERAESTPWPLWP
                     MQLRTSHAHEEGCDRQWSVSTTKFTGHDGHVTKLHAHRVRFSNGKFASVSDTDFEMNV
                     DLVLLAMGFTGPVKNGLLDNLGVKYDARGAVAVDENLMTDREGVFAGGDTKRGASLIV
                     WAIAEGRKMAAGIDKYLQTGKSAKAVA"
     gene            complement(3845..4633)
                     /locus_tag="VN647_06275"
     CDS             complement(3845..4633)
                     /locus_tag="VN647_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HWG96672.1"
                     /translation="MLITETSEEGSRIAGSLVAQTIMQTPALRLGLAAGNTPIGLYRN
                     LVDLHRTTHLDFSRVRLFSLDEFIGLRSDSPNHYRVFYHQHLIDHLNIEPAHLHLLSG
                     PAEQGIASSCVSYERLIQNQGGIDLQILGIGRNGHLGFNEPGSSLNSRTRRILLSAST
                     KQNLGRVFYGEPVPEWAVTMGLGTIREARTLLLLAFGAHKAEVVAKAVEGPLSAFTPA
                     SSIQLHPSVVLVLDREAAGSLTNQEYYRQQSSQVMKGDQWFLEL"
BASE COUNT         1129 a         1348 c         1505 g          998 t
ORIGIN      
        1 tcgatttcgg cggcgagatc cactaccgca tccagggaga gcaccataac tggaatccgg
       61 agacgatcta caagctgcag cacgccagcc gctcgaacga cccgaagacc tacgccgaat
      121 tttcgcagat tgtgaacgac gagagtaagc agcggtcgaa cttgcgcggt ctgctggatt
      181 tcaagttcct tccccggccg attccgatcg aagaagtgga atcggccaaa gaaatcgtca
      241 aacgattcaa caccggtgcg atgtccttcg gttcgatcag caaggaagcg cacgaaacgc
      301 tggcgatcgc catgaaccgg ctcggcggca agagcaacac cggcgaaggc ggcgaagatc
      361 ccgagcggtt cattccgctt cccaacgggg attccaagaa cagttacatc aaacaagtgg
      421 cgtcggcccg attcggagtc acggcgcatt acctcgcgaa tgcgcgggaa ttgcagatca
      481 aaatggcgca gggagccaag ccaggcgaag gcggccagct gccgggacac aaggtggacg
      541 aaaacatcgc caaatttcgc tatgcgactc ctggcgtaca gttgatctcg ccgccaccgc
      601 atcacgatat ctattcgatt gaggatttgg cccagctcat cttcgacttg aagaattcca
      661 atccggatgc cggtgtatcg gtgaaactgg tggcggaggt cggcgtcggg accgtcgcgg
      721 ccggtgtcgc caaggcccat gcggacaagg tgctcatcag cggtgattcg ggcggcacag
      781 gcgcatcacc gttgtcgtcg atcaaatatg cggggatccc ttgggaactg gggctggcgg
      841 aaactcatca gacattggtc ctcaacgatc tgcgcgggcg tatccgtgtt gaaaccgatg
      901 gtcagatgaa gaccggccgc gacgtggtca tcgccacgct gctcggtgct gaagagtatg
      961 ggtttgcgac ggccccgctg attgtcgaag gctgtatcat gatgcgcaag tgccatctga
     1021 acacctgtcc ggtcgggatc gcgacacaag atcctgaatt gcgcaagaag ttcaacggca
     1081 agccggaaca catcgtcaac tacctgttct tcgtggccga agaagcgagg cagctgatgg
     1141 cgaggctcgg atttcggacg atcaacgaga tggtcggccg cgtcgacaag ctcaagatca
     1201 ccagagccgt cggccactgg aaagccaagg ggctggacct cacgccgctg ctgacaattc
     1261 ccgatgtttc ggcggatgtg ccgcgctatt gtgtgcagaa gcaggaccat gggctcatcg
     1321 acattctcga taacaagctc gtcgagcttt gcaaggcggc aatcgagagg ggtgagaagg
     1381 tcaagctcga gcttccgatc aggaacatca atcggacgac cggcacggtg ctctccagca
     1441 agattgcaaa aaagtacggg ccggacgggt tgccggaaga cacgatctcg atcaaattta
     1501 ccggctcggc cggtcaatcg ttcggtgcgt ttcttgcgaa aggcatcacg ctgaccctgg
     1561 aaggcgagtc caacgactat atcggtaaag gattgtccgg cggcaagatc atcgtcttcc
     1621 cgccgaagaa tattctttat aatccggaag agacgatttt gatcggcaat acgtcgctct
     1681 atggggcaac gcaaggggag gcctatttct atgggatggc gggtgagcgg tttgccgtca
     1741 gaaacagcgg ggcgcaggcc gtcgtcgaag ggacgggcga tcacgggtgt gaatacatga
     1801 ccggtggtgt ggtggtggtg ctcggcaaga ccgggcgaaa cttcgcggcc ggcatgtccg
     1861 ggggaacggc gttcgtgctg gacgatgccg ggacattcca gagccgctgc aacaccggga
     1921 tggtcgaact ggaaccggtg acgacgaagg acgacaagca gctgctccac ggattgatcg
     1981 ccaagcattt tatgtacacg ggtagtcgta aggccaagca agtgctcgat gcgttcgaag
     2041 ccatcttgcc gaagtttgtg aaggtgatgc cggtggatta caagcgagtc ttggaagaac
     2101 ggaagcggaa ggcaagtgca gtgcactagc tcgcattcgc tcgctatgag gactgagtgc
     2161 tgaggaatga gggccgagtg aagggtagtg catttcttct cagtcctgag tcctcagtcc
     2221 tggagagaag atgggtgatc caaaggggtt catgaaatat gcccgtgagg gcccgaagcg
     2281 caagccgatc gagctgcgtg tgctcgattg gaaggagatg tacgagccga tctccgagga
     2341 caagttgaag actcagggtg cgcgctgtat ggattgcggg gtgccgtttt gccaaggcca
     2401 taccggttgc ccggtcgtga atctgattcc cgaatggaac gatctcgtct atcgcggtcg
     2461 ctggaaagac gcgcttaaag ctctgcatac gacgaacaac tttcccgagt tcaccggcag
     2521 actctgtccg gcgccttgtg agggggcctg tgtgctgggt atcaatgaag atccggtctc
     2581 catccgcatc atcgaatgga atatcgtcga tcgtggcttc aacgatgggc tcgtgactcc
     2641 aatcctgccg gcagtcagga cggggaaatc ggtggcaatc gtcggttccg gtccggccgg
     2701 ccttgccgcc gcacaacagc tggctcgggc gggccatgat gtcacggtct tcgaaaaggc
     2761 cgatcgcatc ggcggcctgc ttcgctacgg tattcctgat ttcaagatgg agaagtgggt
     2821 catcgacaga cggctggaac agatgaaggc cgaaggggtg aaatttcaaa ccggtgtcat
     2881 ggtcggcgaa gacatcaccg gcgaacagct gcgtcaacgg tttgatgcag taggtcttac
     2941 gatgggagcc gaacaagcgc gtgagttgcc cattcccgga cgcgagttga agggcgtgca
     3001 tcttgcgatg gaatatctca cccagcaaaa taagcgcacg gcaggcatcc ctttcagcga
     3061 tgaaccgatc accgcgaagg gtaaacgggt ggttatcatc ggaggaggcg atacaggctc
     3121 cgactgtctc ggcaccgcgc accgtcaagg ctgtctcgaa gcgcatcaat ttgaactgct
     3181 gccagagccg ccgccggagc gggccgagtc gaccccctgg ccgctctggc cgatgcagct
     3241 gcgcacgtcg catgcacatg aagaaggctg tgatcggcag tggagcgtct ccaccacgaa
     3301 gtttaccggc cacgatggtc atgtcaccaa actgcatgcg catcgcgtac ggttcagtaa
     3361 cggtaaattc gcatctgtct ccgacaccga cttcgagatg aatgtcgatc tggtgctgct
     3421 cgccatggga ttcaccggtc ccgtcaagaa tggcctgctc gacaatctgg gcgtgaagta
     3481 tgacgcgcgc ggagccgtgg cggtggacga gaacctcatg accgaccgtg aaggcgtctt
     3541 cgccggcggc gataccaaac gcggtgcctc actcatcgtc tgggccatcg ccgaaggccg
     3601 caagatggcg gcggggatcg ataagtatct gcagactgga aaatccgcga aagcagtcgc
     3661 ctaaaaggcc tcgcaacata aagtccaagt ttcttctcca tatttactgc ttccttctca
     3721 cccgcccacc ccatcgcgct aaagacgcga ctttcaccgg accggttctt gccttgcgca
     3781 aacccataac ggcggattcg gtgggagatc aaaggtctta cccgctgtga gtagcctgcg
     3841 taaatcaaag ttccaggaac cattgatcgc ctttcatcac ctgagacgat tgctgccgat
     3901 agtattcctg attggtgaga gaacccgctg cttcccgatc cagcacgagg acgacactcg
     3961 gatgcaattg aatggaggag gcaggtgtga aagcgctcaa cggtccttcg acggctttgg
     4021 cgacgacctc ggccttatgt gctccaaagg ccaacagcaa gagagttcgc gcctcacgaa
     4081 tagtaccgag acccatggtt acagcccatt ccgggaccgg ctcgccataa aagacccgac
     4141 caagattctg tttggtcgac gcgctcagga gaatgcgcct cgttcgggaa ttgaggctgg
     4201 aacctggttc attgaatcca aggtggccgt tccgtccgat ccccaagatc tgcagatcga
     4261 taccgccttg gttttggatg agccgctcat aggaaacaca ggaggaggcg atgccctgct
     4321 cggccggccc gctcaacaga tggagatgag ccggctcgat attgaggtgg tcgatcaaat
     4381 gttgatgata gaacacacga tagtgattcg ggctgtcgct ccgcaacccg atgaactcgt
     4441 cgaggctaaa aagccggacg cgagaaaaat cgagatgcgt cgtgcggtgt aggtccacca
     4501 agttccggta caacccgatc ggcgtgttgc cggcagcgag tccgagccgt aatgccgggg
     4561 tttgcatgat cgtctgggcg acgaggcttc ccgctatcct gctgccttcc tctgatgttt
     4621 ctgtgatgag caccagcatc attcattcga aaagaatgat tcattcaccg acgattcacg
     4681 gatcatttgt tcatacagtt ggacgtattc tttcaccatg cattcgcccg tgaaccgctc
     4741 ctcgaaggct ctgcggcatc ggcggcggtc gatctcgccg acgcggtcgc cggctccagc
     4801 ttttacgctt ttaaacttgg gtacctctcc agagggtacc catcgaggcg ttacagcagg
     4861 atctagttca gcggatgaac gactccggcg aaggtcagca gatcggtgat ctcgaaatct
     4921 ccaacttttg ccgacggcaa ggtcggtttc cagttcttat cgacaacgag atacgagtcc
//
LOCUS       DATNDD010000263         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_116451, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000263 DATNDD010000000
VERSION     DATNDD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116451"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VN647_06280"
     CDS             complement(<1..998)
                     /locus_tag="VN647_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWG96673.1"
                     /translation="MFIPRRRFLGSTALVLGNALLDKLTTPLWASTTPAQVETTVAST
                     PSSPVQFIDVAERAGLKVPNVWGGVEHKRVIIETKGSGIAFFDYDNDGWLDIYLTNGS
                     RLDTKWPVGAAPTTHLFKNNRDGTFTDVTEKSGLGRSGWQTGVCVGDYDNDGWDDLFC
                     TFWGHNILFHNNGNGTFADVTGKAGLTQTKGRWGTGCSFVDYDRDGNLDLFVCNFVHL
                     DPDNPPSIGSASFCQWKGVPTMCGPRGLPGDTNILYRNKGDGTFIDVSEKAGILKPGP
                     RYSITSVCCDFDNDGWPDIYVAVDSMPSILYQNNHDGTFTDIAVMAGCAYNADGHE"
     gene            complement(1068..>2545)
                     /locus_tag="VN647_06285"
     CDS             complement(1068..>2545)
                     /locus_tag="VN647_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HWG96674.1"
                     /translation="FVQSGATDAYSPAHPLRSALFHNNGDGTFSDVTNKAHVGGEGHY
                     GQGVAVGDFNNDGYPDLYVTGYGRAILYRNNGDGTFTDVTAPSGVGDDGNWSSSAGWF
                     DFDKDGWLDLVVTNYIDWSPKGNIWCGERKPGYRSYCNPNNYRGQKTKLYHNNHDGTF
                     TDVSEKSGVGVPESKGMSMVLADLNNDGWPDIAIANDTWPNFFFENSHDGTFKDVSLI
                     SGLAASEDGRYEAGMGIDAADVDGDGNLDIYITHLDFELNRLYHNNGDGTFTDVTYSS
                     GIGNKAILLSGVSAKFIDYDNDGWPDILQANGAMIDNVQLYHSLVSYKEPLLMFRNLG
                     HGRFEKVSESLGPDFNRPMPGRGLATADFFNDGQVGIAVNCRGESPLVLRNEGTPNHW
                     LEVLLIGTTSNRDGIGARLKLSAGEVVQVDQAKGGTSYMSASDPRIHFGLGSRKKIDS
                     LQITWPSGHVDKLADVPVDSIIAVKEGVGIVSHPFPKVTPR"
BASE COUNT          567 a          683 c          696 g          599 t
ORIGIN      
        1 tgctcgtggc catctgcgtt ataagcgcag cctgccatca ctgcgatatc cgtaaaagtg
       61 ccatcatggt tgttctgata gaggatgctc ggcatggaat caacggcaac atagatatcc
      121 ggccagccgt cgttatcgaa atcgcagcag acggacgtga tcgaatagcg tggaccgggt
      181 ttcaggatcc cggctttttc cgagacatcg atgaaagtac catctccctt gttgcgataa
      241 aggatattgg tgtcgccagg aaggccgcgt ggcccgcaca tggtagggac acctttccac
      301 tggcagaagc ttgcgcttcc gatggacgga gggttgtcgg ggtcgagatg cacgaagttg
      361 cagacaaaga ggtccagatt gccgtctcga tcgtagtcga cgaaactgca gccggttccc
      421 caacggcctt tcgtttgggt gagccctgct tttcccgtga cgtcggcgaa agttccgttg
      481 ccattgttgt ggaataggat gttgtgtccc cagaacgtgc agaagagatc gtcccatccg
      541 tcgttgtcgt agtcgccaac gcagacacct gtttgccagc cgctgcgacc gagtcctgat
      601 ttctcggtca catccgtgaa tgttccgtcg cggttattct taaacaaatg cgtagtaggt
      661 gcggctccaa cgggccactt ggtatcgagc ctgctgccat ttgtgaggta aatgtccagc
      721 caaccatcgt tgtcatagtc aaagaaggcg atgccgctcc ccttcgtctc gatgatgacc
      781 cgtttatgtt caacaccgcc ccatacgttc ggcactttta gaccggcacg ctcggccaca
      841 tcaatgaatt ggacggggga cgacggagta gacgctactg ttgtctccac ctgagctgga
      901 gttgtgctgg cccacagggg cgtcgtgagc ttgtcaagca gcgcgttgcc tagaactaac
      961 gcggtcgatc cgaggaagcg gcgacgagga atgaacatgc gattcagtca ttataagagt
     1021 ggtcaattgg taatggaacc ccgcaagaga tatgacaggc atccgaatca acgaggtgtg
     1081 accttgggaa acggatgaga gacgatgccg actccttcct ttacagcgat gatggaatca
     1141 actgggacat ccgctagctt gtccacatga ccgcttggcc acgtgatctg aagagagtcg
     1201 atcttctttc gactgccgag accaaagtga atacggggat cactggcgga catgtagctg
     1261 gtccctcctt tcgcctgatc tacctggacg acctcgccgg ctgaaagctt gagacgcgct
     1321 ccgattccat cgcgattgga ggtcgttcca ataaggagga cttcaagcca gtgatttggg
     1381 gtgccttcat tccgcaaaac cagcggagac tcgccacggc aatttaccgc tatcccaacc
     1441 tggccgtcgt tgaagaaatc ggcggttgca agtccgcgtc cgggcatggg gcgattgaaa
     1501 tcggggccca atgactcaga gaccttctca aaccggccat gaccgagatt gcggaacatg
     1561 agcagcggct ctttgtagct gacgaggctg tggtagagct gtacgttgtc gatcattgct
     1621 ccattcgcct gcagaatgtc aggccatccg tcgttgtcgt agtcgataaa cttggcagac
     1681 actccactga gcaagattgc tttattgccg ataccggagc tataggtcac atcggtgaag
     1741 gttccgtccc cgttgttgtg atagaggcga ttcagctcaa aatccaagtg agtaatgtag
     1801 atgtccaaat tcccatctcc atcaacatca gcggcatcga tgcccatgcc ggcttcatac
     1861 cggccatctt cactggcagc aagtccggaa atcagcgaaa cgtccttgaa ggtcccgtcg
     1921 tggctatttt caaaaaagaa attgggccat gtatcgttgg cgatcgcaat gtccggccag
     1981 ccatcgttgt tcaagtccgc gagcaccatg ctcattcctt tggattccgg gactcctaca
     2041 cccgactttt cgctcacgtc ggtgaaggtg ccgtcgtgat tgttgtgata gagctttgtc
     2101 ttctggccgc ggtaattatt tggattgcag taggaccgat aaccaggttt gcgttctcca
     2161 caccagatat tgcccttcgg agaccaatcg atgtagttcg tgactaccag gtcgagccaa
     2221 ccatctttgt cgaaatcgaa ccagcctgcg ctcgatgacc agttcccatc gtcaccgact
     2281 ccggacggag cggtcacatc cgtgaaggtc ccatcgccat tgttgcgata taggatggct
     2341 cgaccatacc cagttacgta gaggtcagga tagccgtcgt tgttgaaatc gccgaccgcc
     2401 actccttgac catagtgtcc ctcgcccccg acatgagcct tgttggtcac atctgaaaac
     2461 gtgccatcgc cgttgttgtg aaagagcgcc gagcggagag gatgtgccgg tgaatatgcg
     2521 tcagttgcgc ccgattgcac aaaat
//
LOCUS       DATNDD010000264         4698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3625163, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000264 DATNDD010000000
VERSION     DATNDD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625163"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /gene="gyrA"
                     /locus_tag="VN647_06290"
     CDS             complement(<1..2351)
                     /gene="gyrA"
                     /locus_tag="VN647_06290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWG96675.1"
                     /translation="MPHDERLEHIDIEDEMRSSYLSYAMSVIVGRALPDVRDGLKPVH
                     RRILYGINEMGLASNRAYRKSAKIVGEVMGNYHPHGNMPIYDALVRMAQDFNMRYPLI
                     DGQGNYGSMDGDPPAAERYTEARMTKLAEEMLADIDKETVDFDPNYDESRQEPLVLPT
                     RVPNLLINGAGGIAVGYATNIPTHNIAEIIDGLLLLLENPDVTITQLMKKIPGPDFPT
                     AGFIYGMSGIKEAYETGRGLLTLRAKIVVEVDERTERERLIVTEIPYQVNKVSLIKKI
                     AELVQEDRIKGISDLRDESSDREGVRVVIELKRGEIPLVVLNNLYKHTPLETTFGVIM
                     LALVNNRPEILNLKQILHHFLEHRREVVVRRTAFELRKAEERAHILEGLKIALDNLDA
                     VITLIRRSQSPDEARAGLIRQFGLTEIQATAILDMRLQRLTQLERTKLVEEYQEVLKQ
                     IEYLKSVLASEALVRTIIKDELAEIREAYKDERRTQIVKEEAEISLEDLIAAEEVIVT
                     ISHAGYIKRNALSLYRAQRRGGKGKIGMGIKEEDFVENLFTASTHDSLLFFTDAGKVF
                     WLKVHEIPEASRAAKGKALVNLLALSGSEKVTAILPVKEFREDRYIVMGTKKGIIKKT
                     ELSAFSNPRQGGIIALGLEGGDRLIGVQLTDGQREILLGTRQGITIRFKEEEVRPMGR
                     TAYGVKGITLEEGNEVIGMETITPDSTTSVLTVTEGGYGKRTPVGEYRVQGRGGKGII
                     SVKTTERNGLAVGFLQVRDGDEIMLIAAQGKVLRCKVDDIREIG"
     gene            complement(2510..>4698)
                     /locus_tag="VN647_06295"
     CDS             complement(2510..>4698)
                     /locus_tag="VN647_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HWG96676.1"
                     /translation="KKSAAEVALTVLHAGGKFEQGAYTVSGGLHGVGISVVNALSEWL
                     ELEIWQNAQVFEQRYERGKPSAPLQMTGKTKRRGTKVRFKPDGQIFETLEFSFDVLAQ
                     RLRELAFLNKGLAITLRDERKEPAKEQVFLYKGGIVSFVEHLNEAKTPLHKPIYVKVE
                     KPEMILEVALQYNDGYAENLFSFANNINTKEGGTHLVGFKAALTRTINNYANANDLFK
                     KETESLTGDDVREGLTAVVSVKVRNPQFEGQTKAKLGNSEVKGVVEAAVNEALGNYFE
                     ENPPVARKIIGKAVDAARAREAARKAKDLIRRKSALDGGSLPGKLADCSEKDPAFSEL
                     YLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSDEIRTLIMALGTGI
                     GRKREEGDKSEKDTFDIAKARYHKIILMTDADVDGSHIRTLLLTFFFRQMPELLERGY
                     IYIAQPPLFKVKKGKTERYLKDEGSLNEYLADLAVEDVELYTEGAQGYVTGRRLLPIL
                     KKMISFETLLGRLNKKPHEAAMLRAFVDEPGLDRELLKDRETLNRVVIGVKKALLLAF
                     PKLEPTFEIFADEEHQSNKVTCRLHANGMVHSIEINHDLVGSADFRELQKLAPSVIGL
                     GRAPYRLNTKGQERQLVTTAETVKTILELGKQGLGIQRYKGLGEMNPSQLWETTMDPE
                     KRTLLKVQLEDVTGVDEIFTILMGDEVEPRRNFIQEHALEVRNLDV"
BASE COUNT          914 a         1365 c         1204 g         1215 t
ORIGIN      
        1 ccgatttcac ggatgtcgtc caccttgcaa cgaagcactt tcccctgcgc ggcgatcaac
       61 atgatttcgt cgccgtctcg tacttgcaga aatccgacag ccaagccgtt tcgctctgtt
      121 gtctttacgc tgatgatgcc cttgccgccg cggccttgca cacgatattc gcccactggg
      181 gtccgtttgc cgtagccccc ttccgtgacg gtcaaaaccg atgtcgtgga atcgggggtg
      241 atggtttcca tgccgatgac ttcattgcct tcttcgagcg tgatcccttt gacaccatac
      301 gcggtccggc ccatcggcct cacttcttct tctttaaacc gaatggtaat accctgtcgg
      361 gtccccagaa gaatttcccg ctgtccatcg gtcagttgca cgccgatcag tctatcgccg
      421 ccctcaagtc ccaacgcgat gataccacct tgccgtggat tgctgaaggc ggacagttcc
      481 gtctttttga tgatgccctt cttggtaccc atcacgatat agcggtcctc tcggaactct
      541 ttcacgggca agattgcggt gaccttttca ctgcctgaca gggcgagcag attgacaagg
      601 gctttgccct tcgcagcgcg gctggcctct ggaatttcgt ggactttcag ccaaaagacc
      661 tttcccgcat ccgtgaaaaa gaggagtgaa tcatgggtag aggcggtaaa gagattttcg
      721 acaaaatcct cttccttgat gcccattccg atctttccct taccgcctcg tcgctgcgcg
      781 cgatagaggg atagggcatt tcgtttgatg tatccggcgt gcgagatggt cacgatcact
      841 tcttctgctg cgatcaggtc ttccaggctg atctccgcct cttctttgac gatctgtgtc
      901 cgccgttcgt ccttataggc ttctcggatc tcggccagct catctttgat gatggtccgc
      961 accaaggcct cgctggccag aacggatttc agatattcga tctgcttgag aacctcttga
     1021 tattcttcga ccagtttggt tcgctcgagc tgtgtcaagc gctgaagtcg catgtcgaga
     1081 atcgcggtgg cttggatttc ggtaaggccg aactgtcgga tcaagccggc ccgcgcttcg
     1141 tcgggcgatt gcgacctgcg tatgagcgtg atcacagcat cgaggttgtc gagcgcaatt
     1201 ttaagccctt ccagaatgtg ggcccgctct tcagcttttc gtagctcgaa agccgtccgg
     1261 cgcaccacga cttcgcgacg atgctcaaga aaatgatgaa ggatctgctt gaggttcaga
     1321 atctccggac gattgttcac cagcgccaac ataatgacgc cgaaggtcgt ttcaagtggt
     1381 gtgtgtttgt acagattgtt caacacgacc agcgggatct cacctcgttt gagctcgatg
     1441 accacccgca ccccttcgcg atccgatgat tcgtctcgca agtcggagat gcccttgatc
     1501 cggtcttcct gaaccaattc ggcaatcttc ttgatcaaag acaccttgtt gacttggtat
     1561 ggaatttccg tgacgatgag gcgttcgcgt tcggtgcgtt catcgacttc caccacgatc
     1621 ttcgccctca aggtgagaag gcctcggccg gtctcgtagg cttctttgat tccactcatg
     1681 ccatagatga atccggccgt ggggaaatca gggcccggga tcttcttcat caactgggtg
     1741 atcgtcacgt ccggattctc caacagcagg agcaacccat caatgatctc ggcgatattg
     1801 tgcgtcggga tgttcgtggc atagccgaca gcgatgccgc ctgctccatt aatgaggaga
     1861 ttcggcaccc tggtcggtag aaccaggggt tcctgtctcg attcgtcgta gttcggatcg
     1921 aaatcgaccg tttctttgtc gatgtcggcc aacatctctt cggccagttt ggtcatgcgc
     1981 gcttcggtat accgttctgc ggccggcgga tcaccatcca tcgacccata attgccctga
     2041 ccgtcgatga gcggatagcg catgttgaag tcttgcgcca ttcgaacgag cgcgtcgtag
     2101 atgggcatgt tcccatgcgg atgataattc cccatgacct cgcccacgat cttggccgac
     2161 ttgcggtagg cccgattgga ggcgagaccc atttcattta tgccgtacag gatgcggcga
     2221 tgaacaggtt tgagcccatc acggacgtcg ggcagtgctc ttcctacgat gacgctcatc
     2281 gcataactca agtacgacga gcgcatttca tcttcgatgt cgatatgttc taaacgttca
     2341 tcgtgaggca ttctgtcctc ggatgctgaa aataatcacc gacttcggtc tcggatcgaa
     2401 agatcctcaa cgtacccaga gggtacacct gcggccttac cggatgaccc attgagcatc
     2461 cgacaagatt aatgcagagt gcttgtttct aaacggtctc gccgactccc tacacgtcca
     2521 agttccggac ttcgagcgca tgttcctgga tgaaatttct tcgcggctcg acttcgtcgc
     2581 ccatcagaat cgtaaagatt tcatcgacgc cggtcacgtc ctccagctga accttcaaga
     2641 gcgtgcgttt ctcagggtcc atcgtcgttt cccacaattg ggacgggttc atttcaccaa
     2701 gtcctttgta ccgctgaata ccgagacctt gttttccaag ttcgaggatc gtcttgaccg
     2761 tttcagctgt agtcaccaac tgccgctcct ggcccttagt gttcagcctg tacggagccc
     2821 gacccagtcc gatgactgac ggcgcgagct tttgaagctc ccgaaaatcg gccgatccca
     2881 ccaggtcatg gttgatctcg atgctatgca ccatgccgtt ggcatgaagt cggcacgtaa
     2941 ccttgttcga ctgatgttct tcatccgcaa aaatctcgaa cgtcggttct aacttcggga
     3001 acgcgaggag gagtgccttc tttaccccaa tcaccacgcg attgagtgtc tctcgatcct
     3061 tgaggagctc gcgatcaagc cctggctcat cgacaaaggc gcgcagcatg gctgcttcat
     3121 gcggcttttt attgaggcga ccgagcaacg tttcgaacga gatcatcttc ttgagaatcg
     3181 gcaacagccg acgccctgtc acatatccct gggcaccttc cgtgtacagt tcgacgtcct
     3241 caaccgccaa gtcggccaag tactcgttca acgacccctc atccttgaga taccgttctg
     3301 tcttgccttt ttttactttg aacagcggtg gttgagcgat gtagatatag ccccgttcga
     3361 gcaattcagg catctgtcgg aagaagaacg tcaagagcaa ggtacggatg tggctgccgt
     3421 ccacgtcggc atcggtcatg aggatgatct tgtgataacg agcctttgcg atatcgaacg
     3481 tatctttctc cgacttgtcg ccttcctcgc gcttgcgacc gatcccggtc cccaaggcca
     3541 tgatgagcgt cctgatctcg tcgctcgata acatcttgtc gaaacgtgcc ttctcgacgt
     3601 tcaggatctt tcctttcagc ggcaagatgg cttggaactt gcggtcgcgt ccttgcttcg
     3661 cggacccgcc ggctgaatca ccttcaacga ggtagagttc gctgaaggcc ggatcttttt
     3721 ccgaacaatc cgccaacttt ccgggaagcg aaccaccgtc gagcgcgctc ttgcgtctga
     3781 tcagatcctt ggctttccga gcggcttcgc gcgcacgggc cgcgtcgaca gccttgccga
     3841 tgatctttcg agcgacagga ggattttctt cgaaataatt gcccaaggct tcgttgacag
     3901 cagcctcgac aacgcctttc acttcgctgt tcccgagctt ggctttcgtc tggccctcga
     3961 actgcggatt acggaccttg acgctgacga ccgccgtgag accctcccgc acatcatctc
     4021 cggtcagtga ttcggtttcc ttcttgaaga gatcgtttgc attggcgtaa ttattgatcg
     4081 tcctcgtgag ggcggcttta aaccccacca aatgggtacc gccttccttg gtattgatgt
     4141 tgttcgcgaa tgaaaatagg ttctccgcgt atccgtcgtt gtactggagc gccacttcca
     4201 gaatcatttc cggcttctca actttcacat agatgggttt gtgcagcggc gttttcgcct
     4261 cgttcaaatg ttccacgaaa gagacgatgc cgcccttgta taggaaaact tgctctttcg
     4321 ccggttcttt ccgctcgtct ctcagagtga tcgccaggcc tttgttcaaa aaggccagct
     4381 cgcgtaggcg ttgcgccaac acgtcgaagc tgaattcgag cgtctcaaag atttggccgt
     4441 ccggcttgaa gcgaaccttg gtccctcggc gtttagtctt gcccgtcatc tggagcggcg
     4501 cgctgggctt gcctcgttca tatcgttgtt cgaacacctg ggcattctgc cagatctcca
     4561 actcgagcca ctccgataag gcgttcacga cggagatgcc gacgccgtgt aggccgccgg
     4621 agacggtgta ggctccctgc tcgaatttgc cgcctgcatg aagcacggtc agcgccacct
     4681 cggccgccga tttctttt
//
LOCUS       DATNDD010000265         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2052971, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000265 DATNDD010000000
VERSION     DATNDD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052971"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2159
                     /gene="lptD"
                     /locus_tag="VN647_06300"
     CDS             <1..2159
                     /gene="lptD"
                     /locus_tag="VN647_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWG96677.1"
                     /translation="VTAERMELNVNTEAGVVTHGQLYVPKTNSLISGRLLQRFSEYHY
                     RVKEGSFTNCDAQDGEVPAWRFRFKDLDMTLGDTLGFKSAWFCVLDVPTIPLPTFSYP
                     MIKRRTGFLMPIPSYDNRFGFHVKQGFFWAINPSHDLTVTPSYYSDLGYGSDFEYRYA
                     LDWRSRGKWYLSYLQQTQLPNVAGVTDTGQNAEKARAALVGSHTQYITDTLLLRANAN
                     LVTDPNYFQQLSNSGVLRALPSTESNLLMTQRLPYGNLYLVGIYLQPLQAGGKDTFQR
                     LPEAGYSLPYTSVFNSPVLLGMDGTFVNFYRDQGFTLNRIDMVPGISTDVIHLGHAIG
                     IRPQAKFREVYYTRGAQSDEAQHRETFWLGVEATSKVTRRFALADGDSLLHTVEPSVT
                     YEYVPSTDQSKLTQIDQVDDLPKKNLLTYMLRSRVLESGGKNAFNWLDLTLAQSYHVG
                     DVQTLARDFTPGVTPFLGSLTQPLQPATTPIQGKKFSDIWMRAVIGNNLPTLMTSAWL
                     DAPVGGRAAQAWALRPPINRYLTVDAFFDPYQPGVSQFNTDLRFQEGTNWYFEVGQRY
                     TRDGNRVRRGDLWNPISFNEVYAPTKEIEFLTMGGAFRTPWGWTFGAKAYYDVKNGSS
                     PQLDAVALYQNPCKCWSVGLYYLKFPDRQQYSFMFSLTGVGWTEGTGTTVMRTLLNPL
                     LWGERGLPWATLGGPYGLPPQTSATGDVPPGVQTGR"
     gene            2249..>2549
                     /gene="trxA"
                     /locus_tag="VN647_06305"
     CDS             2249..>2549
                     /gene="trxA"
                     /locus_tag="VN647_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWG96678.1"
                     /translation="MAGDALKVEDSSWDAEVMKASELVMVDFWAVWCGPCQMVAPIVD
                     ELAKEYAGKLKVRKLNTDENPEVAGRYQVMSIPTILFFKNGQPVEKLVGARPKRQF"
BASE COUNT          578 a          646 c          724 g          601 t
ORIGIN      
        1 atgtgacggc tgagcggatg gaactcaatg tcaatacgga ggccggcgtt gtgacacatg
       61 gccagctcta cgtcccgaag actaactcat taatatccgg tcgcctcctg cagagatttt
      121 ccgaatacca ttaccgagtc aaagagggca gtttcacgaa ttgcgatgcg caagacggag
      181 aagtgccggc ctggcgcttt cgatttaagg atctggatat gaccctcgga gacacgttgg
      241 gattcaagag cgcatggttc tgcgtgttag acgtgcccac gattccatta cccacgttct
      301 cctatccgat gatcaaacgg cgaaccggat ttctgatgcc gatcccgagc tatgacaatc
      361 gattcggatt ccacgtgaaa cagggctttt tttgggcaat caatccgagt catgacctga
      421 cggtgacgcc gtcgtattac agtgacctgg ggtatgggtc cgattttgag tatcggtatg
      481 ccttggactg gcgatctcgt ggcaagtggt atctgagtta tctacaacag acccaacttc
      541 ccaacgttgc cggagtgacc gacaccgggc aaaatgccga gaaggcacgc gcggcgctcg
      601 tcgggagcca tacgcaatat atcaccgata cgttgctgct ccgtgccaac gccaatttgg
      661 tcacagatcc caactatttc caacaactga gcaattcggg ggtactccga gccttaccga
      721 gcactgaatc aaatctcttg atgactcagc gcttgcccta cggcaacctc tatctcgtgg
      781 gcatttatct gcaaccgtta caagccgggg gaaaggatac gttccaacgt cttccggagg
      841 ccggctatag ccttccctat acctcggtgt tcaattcgcc tgtcttattg ggcatggatg
      901 ggacctttgt gaatttttat cgagatcaag gatttacgct taatcggatc gatatggttc
      961 cgggtatttc gacggacgtg attcatctcg ggcatgccat tgggattcga ccgcaagcga
     1021 agtttcgtga ggtctactac accagagggg cgcaatcgga tgaagcgcag catcgagaaa
     1081 ctttttggtt gggggtggag gccacatcga aggtgacacg ccgatttgcc cttgctgacg
     1141 gggacagtct tttacataca gtggaaccct cggtcacgta cgaatatgtc ccatcgaccg
     1201 atcaatcgaa gttgacgcaa atcgaccaag tcgatgacct acccaagaag aatctgctga
     1261 cctatatgtt gcgtagtcgc gtgctggaat caggcggaaa aaatgcgttc aactggctcg
     1321 atctcacgtt ggctcagagc tatcatgtcg gtgatgtgca gactctggcc cgggatttca
     1381 cgccgggagt tacccctttc ctcggatcgt tgacccagcc gctccagccg gccacgacgc
     1441 ccatccaagg caagaagttt tctgatatct ggatgcgtgc cgtgatcggc aacaacctgc
     1501 cgaccctgat gacgtcggcg tggctggatg ctccggtagg cggacgcgcg gcgcaggcgt
     1561 gggctctccg tccgccgatc aaccggtatc tgacggtcga tgcgttcttc gacccctatc
     1621 aacctggggt gagtcaattc aataccgacc ttcgatttca agagggaacc aattggtatt
     1681 ttgaagtggg acaacggtat acgagagatg ggaatcgggt caggcgagga gacctttgga
     1741 acccgatctc gtttaacgaa gtgtatgctc cgacgaagga aatcgagttt cttacgatgg
     1801 gtggcgcatt ccgcactcct tggggttgga cattcggggc gaaggcctat tacgacgtca
     1861 aaaatggaag cagtcctcag ttggatgcgg ttgcgctcta tcagaatccg tgcaagtgtt
     1921 ggtcggtggg gctgtattac ctgaagtttc cggatcgcca gcaatacagc tttatgttca
     1981 gtctcaccgg ggtagggtgg accgaaggca ccgggactac cgtcatgcgg actctcttga
     2041 atccgcttct gtggggtgaa cgtggtctgc cttgggcgac gctgggcggg ccctatggtc
     2101 ttcctcctca gacctccgcg acaggagatg taccgccagg ggttcaaacc ggacggtgat
     2161 ccgtgtttga cactaaaaac agtggtgggg tacgatgctt tgttaattac ccataatgat
     2221 gatctcaatc gtgaaggagg gcgcagacgt ggctggtgat gccttgaaag ttgaagattc
     2281 cagttgggat gccgaggtca tgaaggcttc cgaactcgtc atggtcgatt tttgggctgt
     2341 gtggtgcggc ccctgccaaa tggtggctcc catcgtcgac gagctggcaa aagaatatgc
     2401 cgggaagctg aaggttcgaa aactgaacac cgatgaaaat ccagaggttg cgggtcgtta
     2461 ccaggtgatg agtatcccca cgattctttt cttcaaaaac ggccagccgg tcgagaagct
     2521 ggtgggtgcc agaccaaaac gccaattca
//
LOCUS       DATNDD010000266         5091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1360651, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000266 DATNDD010000000
VERSION     DATNDD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360651"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VN647_06310"
     CDS             <1..323
                     /locus_tag="VN647_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWG96679.1"
                     /translation="TKVAKKVERAETDTALSGFAVEDLDQETAKELGLSGKRGVVVTR
                     VAPDSGAEKAGLMPGDVIREINRQPIKSVKDFEKVSSDVKKGGNVLILVTRRGNSLFL
                     SAKV"
     gene            complement(384..1250)
                     /gene="purU"
                     /locus_tag="VN647_06315"
     CDS             complement(384..1250)
                     /gene="purU"
                     /locus_tag="VN647_06315"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWG96680.1"
                     /translation="MTEQRKDSIILLIHCKDRKGIVARVSGFIHDFGGNILDSDHHTD
                     EETNDFLMRMEFSTEGFQIPSEDIPAAFAPVAKVYEMHYEVYVSSRRTRVALLVSKQD
                     HCLADLLQRHRRDELHIDIPVIISNHDTCVSWADLFKIPFTVYPVTKETKPQQEQQVL
                     MLLKEHRVELVVMARYMQILTADFLAQVGCPVINIHHSFLPAFIGANPYRQAYERGVK
                     IIGATAHYATRDLDEGPIIEQDVIHVGHRDTVEDLVRKGRDLEEIVLARAVRRHIERR
                     VLVYGRKTVVFD"
     gene            complement(1312..2772)
                     /gene="rimO"
                     /locus_tag="VN647_06320"
     CDS             complement(1312..2772)
                     /gene="rimO"
                     /locus_tag="VN647_06320"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWG96681.1"
                     /translation="MSQPLIIPRRAQSVKTAGVRRTTTTIGFVNLGCSKNQVDSEIML
                     GSLVTEGFQLTGDPKQAEVVIVNTCGFIEEAKEESINTILEHGHLKKIGTCRVLIAAG
                     CLAQRYQGDLLKELPELDAVVGTGEFGKIAEICRDLLAPKKRHQRLWISQPPYLYDEL
                     APRLRLGKQHSAYVKIAEGCNRNCTFCAIPLMRGKQRSRPVVSIVAEARQLAAEGVKE
                     INLISQDTVNYGVDLGLREGLVRLLRELVKVKGLRWIRPFYLYPQQVTDDLLDLYAGE
                     EKITKYIDMPLQHINDRMLNRMHRLGNRASIEALVDRIHTRIPGVTFRTAFIVGFPGE
                     TDAAFAELRDYVEQTEFDRVAVFHYSDEEGTGAVDLDEKVEREVMDERRNTLLSIQES
                     ISTVKGRAKIGSVLDVLIDGRSAETEGALEGRHEGLAPEIDGVVYIDEGSTSSFHVCD
                     SRPEMPSPKPGDCCTVEITDAAAYDLVGRIVQNSVC"
     gene            2863..3399
                     /locus_tag="VN647_06325"
     CDS             2863..3399
                     /locus_tag="VN647_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWG96682.1"
                     /translation="MIRTFQGVKPTVPKSCFVETTAVVIGDVVMGEDCSVWFNAVIRG
                     DVNYIRIGERTNIQDLCMLHVTHDTHPLIIGNDVTIGHNVVLHGCIIQDRVLVGMGAI
                     IMDGAVIGEDSVVGAGALIVEGTIVPPKSLILGAPAKMKRPVTEKELAWIKESAENYV
                     KYARQYMDDSTKKTGFVL"
     gene            complement(3424..4119)
                     /locus_tag="VN647_06330"
     CDS             complement(3424..4119)
                     /locus_tag="VN647_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96683.1"
                     /translation="MAIRLSGSTRRAITEVFDHLDYKRLESVYCYEGGDEFWQNKRAP
                     CRRLGIKVAEVLVKKLPHGGRSLYVGAGVTELPSVLAEVLELRRQVEPYNLRRSEVAI
                     LNRACRSFPMRFRAQDAASAPGRFDHLWIVSVLNDPERFPHLSPLSYGRADPVTFDPV
                     RFQKERQIVQSIVDRCMPKLKLPGLVTTSTEEVIWIADWCHRRKVPYRVERKQYPTAL
                     VGDPICFIKVGKG"
     gene            4246..4485
                     /locus_tag="VN647_06335"
     CDS             4246..4485
                     /locus_tag="VN647_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HWG96684.1"
                     /translation="MAPSTQQLLKDALQLPDQQRAELVVELLDSLPPAEPEQARSDAE
                     WFAEIDRRARAAQGGVPGVAWEEARKQVLDRLPKR"
     gene            4482..4814
                     /locus_tag="VN647_06340"
     CDS             4482..4814
                     /locus_tag="VN647_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96685.1"
                     /translation="MKPVGLSPEAVVELAEAASWYETRQPGLAIRFIQEVDQARHAIQ
                     TRPLSFPRLADTASDLQIRRALLPRFPYALVFLELPTEIRVLAVAHAKRAPTTGSTAY
                     EPPETYMV"
     gene            complement(4903..>5091)
                     /locus_tag="VN647_06345"
     CDS             complement(4903..>5091)
                     /locus_tag="VN647_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96686.1"
                     /translation="CELTDGRVLAVEYKGKHLYDGNDAEEKRTVGAVWASRSGGRCLF
                     VMPTQGDFSVITRAVISV"
BASE COUNT         1157 a         1393 c         1397 g         1144 t
ORIGIN      
        1 aaacgaaagt cgccaagaag gtagagcgag ccgaaacgga cactgccttg tccggcttcg
       61 ccgtcgagga tttggatcag gagactgcca aagaactcgg tctcagtggc aagcgaggtg
      121 tcgtggtgac aagggtggct ccggacagcg gagctgagaa ggccgggctc atgccgggcg
      181 atgtcattcg agagatcaac cgacaaccga tcaagtccgt gaaggatttt gagaaggtct
      241 cctcggatgt gaaaaaggga ggcaatgtat tgattctggt cactcggcgt ggcaattcgc
      301 tgttcctatc ggcgaaagtg taacccttac aaaatgaaaa aatgggagca gggtcggtga
      361 cctgctccct tattcgactg ccgctagtcg aacaccaccg tctttctgcc atagactaag
      421 acacgccgtt cgatatgccg ccgcaccgcg cgcgccagca cgatttcctc taagtctcgt
      481 cctttccgca cgagatcctc caccgtatcc cggtgtccca catggatcac gtcttgctcg
      541 atgatcggcc cttcgtccaa atctctggtc gcgtagtgcg ccgtggctcc gatgatcttc
      601 acaccccgtt catacgcctg ccgataagga ttcgctccaa taaaggccgg aagaaaggaa
      661 tggtgaatgt tgatgaccgg gcagcccacc tgcgccaaaa aatccgccgt gagaatctgc
      721 atgtagcggg ccatgacgac gagctccacc cggtgctctt tgaggagcat taaaacctgc
      781 tgctcctgtt ggggcttagt ctccttcgtc actggataga ccgtgaaggg aatcttgaag
      841 agatcggccc agctcacgca ggtgtcgtga ttcgagatga tgacgggaat atcgatgtgc
      901 agttcgtctc ggcggtgtcg ctgaagcaga tcggccagac agtgatcttg tttcgagacc
      961 agcaaagcga cacgagtgcg ccggctggac acatacactt catagtgcat ctcatatact
     1021 ttggcgaccg gagcaaaggc ggcaggaatg tcctcagagg gaatctggaa cccttccgtg
     1081 gaaaattcca ttcgcatgag gaaatcgttc gtctcttcgt cggtatgatg atcggagtcg
     1141 agaatgttcc cgccgaagtc atggatgaat ccagacactc tcgcgacgat gcctttacgg
     1201 tctttgcaat ggatcagcag gatgatggaa tcttttctct gttccgtcat gggttctctc
     1261 ttgtctgatg gcgatcgcta tgagtgtagc aggagcggcg cgaatccacg ctcagcagac
     1321 ggaattctgg acgatgcgtc caacgagatc ataggctgct gcatcggtga tctcgacggt
     1381 gcagcaatcg cccggtttgg gagaaggcat ttcggggcgg gaatcgcata cgtggaagct
     1441 cgaggtcgag ccttcgtcga tgtagacaac accatctatt tcgggcgcga gcccttcatg
     1501 gcgcccttcc agggcgccct ccgtttccgc ggatcgccca tcgatgagca cgtcaaggac
     1561 cgacccgatt ttggccctcc ctttgactgt tgaaatcgac tcttgaatag agaggagggt
     1621 atttcggcgt tcatccatga cttctcgttc gactttctcg tccaagtcga ccgctcccgt
     1681 cccttcctcg tccgagtaat gaaacacagc gactcggtcg aactccgtct gttccacgta
     1741 gtcgcgcagc tcggcaaaag ctgcatcggt ttctccggga aaaccgacga tgaaagcggt
     1801 tcggaaagtc acgccgggaa tgcgggtatg gatacgatcg accaatgctt cgatcgaggc
     1861 gcgattaccg agccgatgca tgcggttgag catccggtcg ttgatgtgct gaagcggcat
     1921 gtcgatatat ttcgtgatct tttcttcgcc ggcatagagg tcgaggaggt catctgtgac
     1981 ttgctgcggg tagagataaa acggacggat ccatcggaga cctttgactt tgaccaattc
     2041 gcgaagtaaa cggaccaggc cctcgcgtag accgagatcc acaccatagt tgaccgtatc
     2101 ttgcgagatc agattgatct ctttgacacc ttcagcagcg agctgccgcg cttcggcgac
     2161 gatcgacact accggcctac tgcgctgctt gccgcgcatc agcggaatgg cgcaaaaggt
     2221 gcaattgcgg ttgcatccct cggcgatttt cacataggcg ctatgttgct tgccgagtct
     2281 caggcgaggt gcgagttcat cgtagagata gggtggttgg ctgatccaga ggcgctgatg
     2341 gcgtttttta ggagccaaca agtctcggca gatctccgca atcttgccga actccccggt
     2401 gccgaccacc gcatccaatt ccggcaactc tttcagcaag tctccttggt accgctgggc
     2461 taaacaaccg gctgcaatca agacacggca ggttcctatt ttcttcagat gtccatgttc
     2521 gaggatggtg ttgatcgatt cctctttggc ctcttcgatg aagccgcagg tattgacgat
     2581 gaccacctcg gcttgtttcg ggtcaccggt cagttgaaac ccctcggtca cgagagaccc
     2641 gagcatgatt tccgagtcga cctgattttt ggagcaaccg agattcacaa atccgatcgt
     2701 agtggttgta cgacggactc cagccgtctt aaccgattgg gcgcgccttg gaatgatcaa
     2761 tggctgagac ataggggcag tctacgggag ggtcaaaaag tctgtcaatt agacccttgt
     2821 agacccttgc gggaggccgt tcacttcacc tacagtgcca acatgattcg cacctttcag
     2881 ggcgtcaagc cgacggtccc gaagtcttgc tttgttgaaa ccaccgccgt cgtcatcggc
     2941 gatgtcgtca tgggtgagga ctgcagtgtg tggtttaacg cggtcattcg gggtgatgtg
     3001 aattatatca gaatcggaga acggacgaat atccaggacc tctgcatgct ccatgtaact
     3061 catgataccc atccgctcat cataggcaat gacgtcacaa tcggacataa tgtggtgctg
     3121 catggttgta taatccagga tcgcgtgctg gttggcatgg gtgcgattat catggacggc
     3181 gcagtgatcg gagaagattc cgtggtagga gctggagcac tcattgtgga gggtacgatc
     3241 gttcccccga agagtctgat cctcggagca ccggctaaga tgaagcgacc ggttacagaa
     3301 aaggagttgg cttggatcaa agaatcggcg gagaattatg tgaagtacgc gaggcagtac
     3361 atggacgatt caaccaagaa aaccggtttt gtgctctgag tgaccgcctt tacgcggcac
     3421 gtctcatccc ttaccaacct tgatgaagca aatcggatcg ccgacgagcg ccgtgggata
     3481 ctgttttcgc tcaacacggt acggaacctt acgacggtga caccagtctg caatccatat
     3541 gacttcctcg gtcgaagtag tcacaaggcc aggcagtttt agctttggca tgcagcgatc
     3601 cacgatggat tggacaatct gtcgttcctt ctggaatcgt actggatcaa acgtgacggg
     3661 gtcagctcgg ccataggaca aaggtgagag gtgcggaaac cgctccggat cgttcaacac
     3721 gctcacgatc cagagatgat caaaacggcc gggcgctgaa gcagcgtcct gagctctaaa
     3781 cctcatcgga aaagatcggc aggcacgatt gagtatcgct acttccgacc gacggagatt
     3841 atagggctca acctgtcgac gaagctcaag cacttccgct aatacggatg gtagctccgt
     3901 caccccggct cccacataca gactgcgccc accatgcggt aacttcttta cgagaacctc
     3961 ggctacctta attcccaatc gccgacatgg cgctcgcttg ttttgccaga actcatcgcc
     4021 cccttcgtag cagtagacgg actccaagcg tttgtaatcg agatgatcaa atacctcggt
     4081 gatcgcccgc cgagttgagc cggataaccg gattgccatg tcgctcgttt atgtcacaaa
     4141 gcctaagaga aaatcgatgc cgggctccct agacttcggg attcatgacc ataaacgagc
     4201 cgatctaacc ttttatttct ctgaaagata ggctaaagtc tccccatggc tccatccacc
     4261 cagcaactcc tcaaagatgc gttgcagctg ccggatcaac agcgtgcgga attagttgtc
     4321 gaattactcg atagcctccc accggcggaa ccggaacaag cgcgcagtga cgcagaatgg
     4381 ttcgcggaaa ttgaccgccg cgcacgggca gcgcagggcg gagtccctgg tgtcgcctgg
     4441 gaagaggcgc gcaagcaggt gttggatcgc ctgcccaaac ggtgaaacct gtcggcctct
     4501 ctcctgaggc agtggtggag ttagccgagg ctgcctcttg gtacgaaacc cgccaacctg
     4561 ggctcgcaat tagattcatt caagaagtcg atcaagcacg acacgcaata caaacccgtc
     4621 ctctgtcgtt tccccggctt gctgatacgg caagcgacct ccagatccgt cgagccttac
     4681 ttcctcgatt tccctatgca cttgtctttc ttgaactccc gactgaaatc cgtgtcctcg
     4741 cagtcgccca tgccaaacgc gccccgacta ctggctcaac cgcttacgaa ccacctgaaa
     4801 cgtacatggt ataacatcca cggtttcata gtttctctct ggaacgtgag gtggggcagg
     4861 gagtgaaaca cgcgggaggc ggcttgctgg gcgagcagtc gatcatacag atatgaccgc
     4921 ccgtgtaatc actgagaagt cgccctgcgt cggcatgacg aacaggcagc ggcctccgct
     4981 acgcgaagcc cacacagcgc cgacagtgcg tttctcttcg gcgtcattgc cgtcgtacag
     5041 atgtttacct ttatactcaa ccgcgagcac acgaccgtcg gttaactcac a
//
LOCUS       DATNDD010000267         4226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3211713, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000267 DATNDD010000000
VERSION     DATNDD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3211713"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..687)
                     /gene="def"
                     /locus_tag="VN647_06350"
     CDS             complement(163..687)
                     /gene="def"
                     /locus_tag="VN647_06350"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWG96687.1"
                     /translation="MLKIAKLGNPILRKVAAPIDPREIKSSEIQRLIDDMFETMYDEP
                     GIGLAAPQVSRSIQLVVMGCNGEGGFPETVLINPSIVYYGPDQVETWEGCLSVDGLRG
                     KVTRPSLVRVKGLDRKGKPLDFEATELYAICIQHELDHLIGKVFLDRMTDMSTLTQLD
                     EFAQYWKKEPTNVI"
     gene            complement(758..1552)
                     /locus_tag="VN647_06355"
     CDS             complement(758..1552)
                     /locus_tag="VN647_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWG96688.1"
                     /translation="MRQSFLFRLGMIWVLGPIGGAELSYAWTDQIQVRQVQMPYEAPP
                     KSQDAPDVAIPPNKNPLSPEELKRTEALLPLLEGKQEFWAMGEFVHLGEPAVPVLIQA
                     LTMPSPRIRYNAIETLLMMKGVAGVPALVTTAKEPGEIPRVREHALRVAVRLDSAKAS
                     EAIEAMAKDPNPSVRKAAAFEARYVRQKAVIPILIPMVSDDERFVALSALQSLWILTR
                     HETEFHDWNTSTKQDRATWSNEWIEWWDANKEIFEIPEPQRPKRSS"
     gene            complement(1599..>4226)
                     /locus_tag="VN647_06360"
     CDS             complement(1599..>4226)
                     /locus_tag="VN647_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWG96689.1"
                     /translation="TDLKTIQDWQVRRAFKRVPGVIDVTTFGGTTKEYHVDIDPGKLI
                     NYGVNLSQVMAALANSNANVGGNYLTLGAQSYNIRGLGLINDLDDIENVMVAEKDGTP
                     IFVKTLGKVTVGHRVRLGKVGIDDRDDAVEGVVLLQRGAQALPVLDKVRVKVEELNGS
                     KLPSGVKIKTFYDRTALINTTVKTVTDILISGMVLVFLILIVFLGDFRAAFIVALTIP
                     LSLLFTFTMMVFVGESANLISLGAIDFGIIVDATLVMVESIFVQLAHRQPHGLTIQQY
                     IIRAAGQVGRPIVFSTAIIVVAFVPLFTMTGVPGKIFAPMSITYGFALFGALLMACTL
                     APVLCSFLLRSSIRETDTAVVAAVRKANARFVVWALEHRFMVVGVATGLVILAFLAFQ
                     SLGGEFMPPLEEGNLWVRATMPVDISFDQADRLTNEIRREFRKSPEVETVVSQLGRPD
                     DGTDPTSFFNGEFLANLKPASEWRRGLSKADLIEEIEHRLNTIPGVIFNFSQVIEDNV
                     EEAMSGVKGENSIKLFGSDLKVMDARAVEIESVMKTVFGVKDLGIFRLIGQPNLLIQV
                     DREASARYGLRVSDVNAVVQAAIGGQAVTQVYEGEKLFDLVVRFLPEYRQDIDSIGNI
                     LVSTPDGARIPLKQVAAITMQTGAFIIYRENNQRYIPIKFSVRDRDLQSTIEEAQEKL
                     ARHVTLPEGYHMEWAGQYDQLKEEQKRLVTIVPVSLVIMLLLLYTTFDSLKNALLVLA
                     AVPFALVGGVLSLVITHTHFSISAAVGMISTLGVAILGGVLLISRIEEFRRAGLELRQ
                     AVIRGVDVQMRPILMATLSAAIGLLPASLATGIGAQAQQPLARVVVGGMLTAAFLILV
                     VLPVLYEIVHQRDNLEEQE"
BASE COUNT          985 a         1263 c         1061 g          917 t
ORIGIN      
        1 atcacatcgt cgatgatgat cttgatgacc cagggaggca ccagctccat cgccgtcgcg
       61 caagcggcgc agacgagcgt cgcgctcgca agagcgcggt ggggtcgtag atacagcaag
      121 acgcgaagaa gggacttcac aaccggctcg acaaggcggg cattaaatta cgtttgtggg
      181 ctcttttttc caatactgcg caaattcgtc aagctgagtg agggtggaca tatcggtcat
      241 ccgatcgaga aagactttgc cgatcaagtg atcgagttcg tgctgaatac agatcgcgta
      301 cagttccgtc gcctcgaaat ccagcggttt gcccttccga tccagcccct tgacccgtac
      361 caatgaaggt ctggtcacct tgcccctcaa tccatcgacg ctcagacacc cttcccaggt
      421 ttccacctgg tcgggcccgt aatacacgat cgaagggttg atgaggaccg tttcgggaaa
      481 tccgccttca ccgttgcagc ccatcacaac caattgaatc gaccgtgaga cttggggagc
      541 ggccagcccg atgcccggtt cgtcatacat cgtttcaaac atgtcgtcga tcagccgttg
      601 gatctcggac gatttgatct cacgcgggtc gatcggagcg gcaactttac gaaggatagg
      661 attacccagc ttggcgatct tcagcatcgt ttcgctcttt aatctcatat tctatcattc
      721 gtaacatagg aatatccgag ctcgcaagct cccccggcta agaacttcgc ttcggccgtt
      781 gcggttcggg aatttcaaag atctccttgt tggcgtccca ccactcgatc cattcgttcg
      841 accacgtcgc acgatcctgt ttggtcgatg tgttccaatc gtggaactcg gtctcatggc
      901 gggtcagaat ccagagtgat tgcaatgccg atagcgccac gaaacgctcg tcgtcgctca
      961 ccatcggaat caagatgggg atgacggcct tctgccggac ataccgcgct tcgaatgccg
     1021 cggcctttct gacggatgga ttgggatctt ttgccatggc ttcaatcgct tcggacgctt
     1081 tcgccgaatc cagacgaacg gcgacgcgca gggcatgttc ccgcacccga ggaatttcgc
     1141 cgggttcctt tgcagtggtg acgagagcgg gcactccggc gactcccttc atcatcagta
     1201 aggtttcaat ggcgttgtac cgaatccgcg ggctcggcat ggtcagtgcc tgaataagaa
     1261 cgggaacagc aggctctccc aaatgtacga attcccccat cgcccagaat tcctgcttcc
     1321 cttcaagcaa cggcaacaag gcttccgtgc gcttcagctc ttcaggactg agcgggttct
     1381 tattgggagg aatcgcaaca tccggcgcat cttgactctt cggcggcgct tcatagggca
     1441 tttgaacttg tcggacctgg atttgatccg tccatgcgta cgatagttcc gctcccccta
     1501 tcggtccaag cacccagatc attcccagtc tgaataggaa agattgcctc acgatgatgg
     1561 cactcctttc tgtattgtcc gacgcatccg tcgactgcct attcctgctc ttcaaggtta
     1621 tcacgctgat gcacgatttc gtagagaacc ggcaacacca cgagaatcaa gaaagccgcc
     1681 gtcagcatcc ctccaaccac cacgcgtgcc aacggctgct gcgcttgagc gccgataccg
     1741 gtcgccagag aagcgggcaa tagtccgatc gccgcgctca aggtggccat cagaatggga
     1801 cgcatttgga catcgacgcc gcgtatcacc gcctgccgca actcaagccc cgcccttcgg
     1861 aactcttcaa tgcgcgagat cagcaacacg ccgcccaaga ttgcgacgcc caaggtcgaa
     1921 atcattccca cggcggctga aatactgaaa tgggtatgag tgatcaccaa cgacaggacg
     1981 ccacccacca acgcaaaggg aacagccgcc aacaccagca acgcattctt caatgagtcg
     2041 aatgtcgtat agagcaacaa cagcatgatg acaaggctca caggaacgat cgtgaccagc
     2101 cgcttctgct cttccttaag ttgatcgtac tgcccggccc attccatgtg gtagccctcg
     2161 ggcagcgtga cgtgccgcgc caatttctcc tgagcttcct ctatcgtact ctgaagatcg
     2221 cgatcccgca cactgaattt gatcgggata taccgctggt tgttttcgcg atagatgatg
     2281 aaggctccgg tctgcatggt aatggccgcc acctgcttga gcggaatgcg cgccccatcc
     2341 ggagtgctca ccagaatatt accgatggaa tcgatatcct gacgatattc aggaagaaac
     2401 ctgacgacca gatcgaacag cttctctccc tcatacacct gggtcaccgc ttgaccgccg
     2461 atggctgcct gaacgaccgc attcacatcg gagacccgca atccataacg ggcgctcgct
     2521 tcgcgatcga cttgaatcag caaattcggt tgcccgatca gccggaagat gcccagatcc
     2581 ttcacgccga aaaccgtttt catcaccgat tcgatctcga ccgccctcgc atccatgact
     2641 ttgagatcgg aaccgaataa tttgatcgag ttctcgcctt tcacaccgga catcgcctcc
     2701 tccacgttat cctcgatgac ctgggaaaaa ttgaaaataa cgccggggat ggtgttcagc
     2761 cgatgttcga tctcttcaat aagatctgct ttgctcaatc ctcgacgcca ttccgatgcg
     2821 ggtttaagat tcgccaaaaa ttccccattg aaaaagctgg tggggtccgt cccatcatcc
     2881 ggccgtccca gttgtgaaac gaccgtttcc acctcgggcg atttccggaa ctcgcgacgg
     2941 atttcgttgg tcagtcgatc ggcctgatcg aaagaaatat cgacgggcat cgtggcccga
     3001 acccataagt tgccttcctc cagaggcggc atgaattcac cacccaacga ttggaatgcc
     3061 agaaacgcga ggatcaccaa gcctgtcgca acacccacca ccatgaacct atgctccaac
     3121 gcccatacga caaatcttgc attggctttg cggacggcag cgaccaccgc cgtatcggtt
     3181 tctctgatcg acgaccgcaa gagaaatgaa cacagcacgg gtgcgagcgt gcatgccatc
     3241 aagagtgcgc cgaacagagc aaacccatag gtaatggaca tgggcgcgaa gattttccca
     3301 gggactccgg tcatcgtaaa aagcggcaca aaagccacca cgatgatcgc cgtggaaaaa
     3361 acaataggcc gtccgacctg gccggcggct cgaatgatgt attgctgaat cgtgagccca
     3421 tgtggttgcc ggtgcgctaa ctggacaaag atgctttcga ccatcaccaa cgtcgcgtcc
     3481 acgatgatcc cgaagtcgat cgcgcccaag gagatcaagt tcgcagactc cccgacgaac
     3541 accatcatcg tgaaggtaaa taacagcgag agcgggatgg tcagtgcgac aataaaggcc
     3601 gcgcggaaat cgccgagaaa tacgatcaag atgagaaaca cgagcaccat gccgctgata
     3661 agaatgtccg tgaccgtctt cacggtcgtg ttgatcaaag ccgttcgatc gtagaacgtc
     3721 ttgatcttga ccccggacgg cagtttcgac ccattcaact cctcgacctt gactcgtact
     3781 ttatcgagga ccggcaacgc ctgagccccg cgctgaagaa gcacaacacc ttccaccgca
     3841 tcatcgcggt catcgatgcc gactttacca agccgcacgc gatgtcctac ggtcaccttt
     3901 cccaaggttt tgacgaagat cggcgtgcca tctttttcag ccaccatcac gttttcaatg
     3961 tcatcgagat cattgatgag gcccaacccc ctaatgttgt aactctgcgc gccgagggtc
     4021 aaatagttgc cgccgacatt ggcgttgctg ttcgccaagg ctgccatcac ttgcgaaaga
     4081 ttgacgccgt agttaatcag tttccccggg tcaatatcga catgatattc tttcgtggtg
     4141 ccgccgaacg tcgtcacgtc gatgaccccc ggcacacgtt tgaaggcccg cctgacctgc
     4201 cagtcctgaa tggttttgag atcggt
//
LOCUS       DATNDD010000268         5937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3801771, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000268 DATNDD010000000
VERSION     DATNDD010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801771"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="nadC"
                     /locus_tag="VN647_06365"
     CDS             <1..606
                     /gene="nadC"
                     /locus_tag="VN647_06365"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWG96690.1"
                     /translation="RSILQAERVALNFLQHLSGIATLTQRFCRAVRGYPAKILDTRKT
                     LPGWRALQKWAVSLGGGINHRRSLSDGILIKDNHLALLQRNRRPVERACRLARAHRSS
                     AIPIIVEAESLAEVRQALAGKPDIILLDNMDPDLVRRAVALIRKQALVEVSGGITLKN
                     VRAMAAAGADRISIGALTHSAPAAMVSLVMASARPLRRRRA"
     gene            610..1449
                     /locus_tag="VN647_06370"
     CDS             610..1449
                     /locus_tag="VN647_06370"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWG96691.1"
                     /translation="MVSSTPLSLDGIRSTLATTSLGHALYLHQDLPSTNREALSLAQN
                     GAQDGTVVIAESQSGGYGRHGRAWFSPPTMNVYCSVIVRGMGGHLSLSQWLSWVPLAS
                     ALAVTEAVQQMAAVSLSLKWPNDLLLHERKVGGILCESSFATTNDPVVVIGIGLNVNV
                     PPMSFPEELQPIAASLMEASRRPIDRNRLIAQLLLELEHCLGELRTHGPVRLRQAYTA
                     RCTTLGRQVRVLFTNEQPIVGIAEALSADGALQVRPLSPHSRSQLMPLVDVRAADVIH
                     LRE"
     gene            1474..2271
                     /locus_tag="VN647_06375"
     CDS             1474..2271
                     /locus_tag="VN647_06375"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWG96692.1"
                     /translation="MNSMLLAIDIGNTNVVAGIFDGLTLLTHWRLATDPKTTADEYGV
                     LCLNLMARDGRVPEQITGAIISSVVPALTETFESMVETVFGCIPVTVSSDMDTGLTLK
                     YSNPKEIGSDRIVNAAAAYEKFHRDLIIVDFGTATTFCAVNRDGEYLGGVIAPGLTIS
                     ADALFTRAAKLSKVELARPKTVIGTDTAGSIQSGLIFGYAGLVDTLIQRMEGEMGRTS
                     YVIATGGLASVIAPEARSIQHIEPFLTLHGLELLYRRAQSASLKQWV"
     gene            2361..2942
                     /gene="grpE"
                     /locus_tag="VN647_06380"
     CDS             2361..2942
                     /gene="grpE"
                     /locus_tag="VN647_06380"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWG96693.1"
                     /translation="MSNDQEQEKINANTIESLEEDSPVTAAGSSFQEELAGKAEECKA
                     LNDKYLRLAAEFENYKRLTQRDQREQIRFGNEQLLKELLPVVDNMERAIKASRTNSGD
                     SGLVQGVELTLKQLSGVLAKFGVQAIETAGQEFDPSAHQAVSYGPSNDVPANKVLDEF
                     QKGYRLHDRILRAAMVSVSSGPAQPNEHGSTTN"
     gene            2984..4903
                     /gene="dnaK"
                     /locus_tag="VN647_06385"
     CDS             2984..4903
                     /gene="dnaK"
                     /locus_tag="VN647_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWG96694.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVAITD
                     KSERLVGQIAKRQAITNPENTIFSVKRLMGRKFKSREVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEISAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDISVLEIGEGVFEVKSTNG
                     DTYLGGDDFDLRVMDWLVDEFRKDQGIDLRKDRMALQRLKESAERAKIELSSSQETEI
                     NLPFITADASGPKHMVTKLTRAKLEQLVDDLIQRTIEPCRKALSDAGVTAKDIQEIVL
                     VGGMTRMPKVIQVVKDFFGKEPHRGVNPDEVVAIGAGIQGGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTVPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPPAPRGMPQIEVTFDIDANGIVHVSAKDLATQKEQSIKITASSGLSKDEVE
                     KLVRDAQSHTEEDKKRRKLAEAKNQADTLVYQTEKNITEYGDKVSAEEKTKIQDAVAT
                     VKKALEGTDAEAIESATQALMTASHKLAEEMYKKAAASPGPQPGASSSDSTGETKTDE
                     KVVDAEFEEVDKDKK"
     gene            4988..>5937
                     /gene="dnaJ"
                     /locus_tag="VN647_06390"
     CDS             4988..>5937
                     /gene="dnaJ"
                     /locus_tag="VN647_06390"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWG96695.1"
                     /translation="MSKRDYYETLGVDRNVSDDELKKAYRKLARQHHPDLHVGDQQKK
                     SAEEKFKEINEAYETLSDQERRKRYDMFGHAGAQHGGSGVDGFDFGRGGFGDVFNDIF
                     EDFFGQARGGGATRAERGNDLQYNLEITFEEAVYGKEAKLKIPRWEICVDCKGTGAKS
                     AAAIKPCASCKGTGQLRFQQGFFSVSRPCGQCEGTGHFVTEPCSTCQGRQRVYKERTI
                     AVHIPAGIETGMRLRLSNEGEHGPNGGPPGDLYVAVTVRPHQVFHRKGLDIACDVPVN
                     LVTAVLGGKVEVPTLTGATVIKVPPGTQHDKVLRIKGLGIP"
BASE COUNT         1423 a         1673 c         1666 g         1175 t
ORIGIN      
        1 cggtccatcc tgcaggccga gcgggtcgcc ttgaactttc ttcaacacct gtccggaatc
       61 gccaccctga cacaacggtt ctgccgggcc gtacgcggct accctgccaa gatcctcgac
      121 accaggaaaa ctcttcccgg ctggcgcgcg cttcagaagt gggccgtttc gctcggcggt
      181 gggatcaacc accgacgatc attgagtgac ggcattctga tcaaagataa ccacctggcc
      241 ctcctccaac gcaatcgacg accggttgaa cgggcatgcc gattggcacg tgcccatcgg
      301 tcaagcgcga tcccgatcat tgtcgaggcg gaatccctcg ctgaggttcg gcaggcgctc
      361 gcagggaagc ctgatattat tctgctggac aacatggatc ccgacctggt cagacgtgct
      421 gtggcattga tcaggaagca ggctctggtg gaggtttcgg gaggcattac tctgaagaac
      481 gtccgagcca tggcagcagc cggcgccgat cgcatctcca tcggagcact cacccattcc
      541 gccccagcag caatggtcag ccttgtcatg gcgtcggctc ggcctttacg acgccggcgt
      601 gcgtagttga tggtgtcctc caccccactc agcctcgatg gtatccgcag cacccttgcg
      661 accacgtcac ttggtcacgc cttgtatctc caccaagatc tcccctcgac caacagggag
      721 gcattgtcgc ttgctcagaa cggcgcacaa gacggcaccg tcgtcattgc tgaaagccag
      781 tcaggcggct acggtcgaca tgggcgcgcc tggttctctc cgccgacaat gaacgtctat
      841 tgctccgtca ttgtacgcgg gatgggggga cacctttccc tttcgcaatg gctatcctgg
      901 gtgccgcttg ccagcgcact ggccgtaacc gaggccgttc aacagatggc ggctgtgtcc
      961 ctttcgctga agtggccgaa tgatctactg ctccatgagc gcaaagtcgg cgggattctc
     1021 tgcgagagca gcttcgccac aactaatgac ccggtcgtcg tcattggaat cgggctcaac
     1081 gtgaatgttc ccccgatgtc ctttccagaa gaattacagc cgattgcggc atcattgatg
     1141 gaagcctcgc gccggccgat cgaccgaaat cgactcattg ctcaactgct tctggaactt
     1201 gaacattgtc ttggcgagct tcggactcat ggaccggtcc ggttacgaca agcctatacg
     1261 gcccgctgta ccacgctggg gcgccaagtt cgcgtcctgt tcacgaatga gcaaccgatc
     1321 gtcggtatcg cagaagctct ctctgccgac ggcgcacttc aggtgagacc attatcacct
     1381 cattctcgct cacaactgat gcctctcgtg gatgtccgtg cagccgatgt cattcatctg
     1441 agagagtagc gaaagagcat caggccgggt agaatgaatt cgatgctctt agccatcgac
     1501 atcgggaata ccaacgttgt cgcaggaatc tttgacggat tgactctatt gactcattgg
     1561 cggttggcga ccgatcccaa aaccactgcc gatgagtacg gcgtcctttg cctcaacctc
     1621 atggcccgag acgggcgcgt ccctgagcag atcaccggtg cgatcatctc cagcgtcgtt
     1681 cccgccctca cggagacgtt tgaatcgatg gttgaaacgg tctttgggtg catccccgtc
     1741 accgtctcat ccgacatgga taccggctta acactgaaat actcgaaccc gaaagagatc
     1801 ggaagcgacc gtattgtgaa tgcggcggcc gcctatgaga agtttcaccg cgatctcatc
     1861 atcgtcgatt ttggtacagc aacgacgttt tgcgcagtca accgagatgg agaatatctt
     1921 ggaggcgtaa tcgcaccggg cctgactatt tccgccgacg ccctgtttac acgggctgca
     1981 aagttgagca aagtcgagct cgctcggcct aagaccgtca ttggaaccga tactgccggt
     2041 agtattcagt cggggctcat cttcggctat gccggtctcg tggacactct aatccagagg
     2101 atggaaggag aaatggggcg cacgtcatat gtgatcgcca cgggtggatt agcctcagta
     2161 atcgcgccgg aggcacgatc gattcaacat attgaaccgt ttttgaccct tcatgggctt
     2221 gaactcctct atcgccgtgc tcagagcgcg agcctgaagc aatgggtcta gtcctctgag
     2281 tactacatca ttttattttc cctttacccg ttgacttccc tcctccctat ggatatctca
     2341 ccgcgtgtat aggtatcagg atgagtaacg atcaagagca ggaaaaaata aacgccaata
     2401 caatcgagag cttagaggaa gattctcctg tcacggctgc gggctcctca tttcaagagg
     2461 aactggccgg caaggctgaa gaatgtaaag cgctcaatga caagtatctc cggctggctg
     2521 cggaattcga aaactacaaa cggctgactc aacgcgacca gcgtgaacaa attcgattcg
     2581 ggaatgaaca gcttcttaaa gagctgttgc cggttgtcga taacatggag cgggcgatca
     2641 aggcttctcg gacgaacagt ggcgattcgg ggcttgtcca gggtgtggaa ctgacgctca
     2701 agcaactatc cggggtcctg gccaaattcg gcgtgcaggc gatcgaaacg gccggccagg
     2761 agttcgaccc aagcgcacat caagctgtct cgtacgggcc gtcgaacgac gtaccggcca
     2821 ataaagtatt ggacgagttt caaaaagggt atcggctgca cgataggatt ctgcgggcgg
     2881 caatggtcag tgtatcgtca ggtccggccc aaccaaacga acatggctca acgacgaact
     2941 gacctgattc tgtcgtcgtt gtcgagaagg aaggagttca ccaatgggta aagtcatcgg
     3001 tatcgatctc gggaccacca actcttgtgt ggcgattatg agcggcggag accccgtcgt
     3061 gatcgccaac gccgaaggga gccgaaccac tccttccgtc gtcgccatta ccgataaaag
     3121 tgaacggctg gtcggccaaa tcgcaaaacg acaggcgatt accaatcccg agaacaccat
     3181 tttctccgtc aagcgcctga tggggcgtaa gttcaagagt cgtgaggttc aagaagccat
     3241 gaagcggctt ccctataagg tcgttgaggc cgacaacggc gatgcgcacg tggaattgcg
     3301 cggcaagcgc tatagcccgc cggaaatctc ggccatgatt ctgcagaaaa tgcggcagac
     3361 cgctgaagac tacctcggtg aaaaggtcac tgaggccgtc gtgactgtcc ccgcctattt
     3421 tgatgacagc caacgtcagg cgactaaaga tgccggtcag atcgccgggc tgaatgtcct
     3481 gcgcatcatc aatgagccga cagctgcctc cctcgcctac ggtctggata agaagaagga
     3541 cgaacggatt gccgtgtacg atttgggcgg cgggactttc gacatctccg tcctggaaat
     3601 cggcgaaggc gtctttgaag taaaatccac caatggtgat acctatctcg gcggagacga
     3661 cttcgatcta cgcgtgatgg attggctggt cgacgagttt aggaaagatc agggcattga
     3721 tttgcggaaa gatcggatgg cgcttcaacg cctgaaggaa tcggcggaac gagccaagat
     3781 cgagctgtcg tcgtctcaag aaacggagat caatctgccg ttcatcaccg ccgatgccag
     3841 cggtcccaag catatggtca ccaagctgac gcgagccaag ctcgaacagc ttgtggacga
     3901 cttgattcag cgcacgatcg agccctgccg aaaagccttg tccgacgcgg gtgtcaccgc
     3961 caaggatatc caagaaatcg tgctggtcgg cggcatgacc cgcatgccca aagtgattca
     4021 ggtggtgaaa gacttctttg ggaaagaacc gcaccgtgga gtgaatcccg acgaagtcgt
     4081 cgccatcgga gccggcatcc aaggcggagt tctcaaagga gaggtcaagg acgtcctgct
     4141 tctcgatgtg actccattgt cgttgggtat tgagacgttg ggcggcgtat tcaccaagct
     4201 catcgaacgc aacacgaccg ttccgaccaa gaagagccaa gtgttctcga cggcagccga
     4261 taaccaaacg gccgtcacca ttcgcgtctt ccaaggcgaa cgggaaatgg ccaacgacaa
     4321 caaactgctc gggcagttcg acttggtcgg cattcctccg gcgcctcgcg gtatgccgca
     4381 gattgaagtg acgttcgaca tcgatgccaa cggcatcgtg cacgtgtcgg ctaaggatct
     4441 ggccacacag aaagaacaat ccatcaagat cacggcttcc agcggcctga gcaaagacga
     4501 agtcgagaag cttgttcggg atgcgcagtc gcacaccgaa gaagacaaga aacgccgcaa
     4561 attggcggaa gccaagaacc aggcggacac tctggtctat caaaccgaga aaaacatcac
     4621 ggaatacggc gacaaagtct cagcggagga aaagacaaaa atccaggatg ccgtcgcaac
     4681 cgtcaaaaaa gcgctcgagg gcaccgatgc cgaggcaatc gaatcggcga cacaagcact
     4741 tatgacggcg tcgcacaagc tagccgaaga gatgtacaag aaagcagccg catctccggg
     4801 tccgcaaccg ggcgcctcgt catccgacag caccggcgaa acaaagaccg atgaaaaagt
     4861 cgtggacgca gaatttgaag aagtagacaa agacaagaaa tagcctagaa tagcgattca
     4921 gagtgcccga gctctcgacg cgatcggaaa ctcgggcggt ttcctacttc catacacaat
     4981 ggccgccgtg tccaaacgcg attactacga aactctcggc gtcgatcgga atgtgtccga
     5041 cgacgagctg aaaaaagcct accggaaact ggcccgccag caccacccgg acctccacgt
     5101 cggcgaccag cagaagaaat ccgccgaaga gaagtttaag gaaatcaacg aagcctatga
     5161 aacgctgagc gatcaggaaa gacggaagcg ctacgacatg ttcggtcatg ccggggcaca
     5221 gcatggcggg tcaggcgttg atgggttcga tttcggccgt ggcggattcg gagacgtgtt
     5281 taacgacatc ttcgaggatt ttttcggcca agcgcgcgga ggcggggcaa ctcgggctga
     5341 acggggcaac gatctccaat acaaccttga aatcacgttt gaggaagccg tctacgggaa
     5401 agaagccaaa ctcaagatcc cgcgctggga aatctgtgtc gactgcaaag ggaccggggc
     5461 gaagtcggcg gcagccatca agccatgtgc cagctgcaaa ggtacggggc aacttcggtt
     5521 tcagcaaggc ttcttcagtg tcagccggcc ctgtggtcaa tgtgaaggca ccggccactt
     5581 tgtcacggaa ccctgctcta cctgccaggg acgtcaacgg gtttataaag agcgcaccat
     5641 cgccgtccat attccggcgg gtattgagac cggcatgcga cttcgtctct ccaatgaagg
     5701 agagcatggt cccaatggcg gccctcccgg cgacctctac gtggctgtta ccgtcagacc
     5761 ccatcaagtc tttcatcgca agggcctcga tatcgcctgt gatgtgccgg tcaacctcgt
     5821 cacggccgtg ctcgggggaa aagtggaagt cccgaccctc acaggagcaa ccgtcattaa
     5881 ggtgccgccc ggcacacaac atgacaaggt ccttcggatt aaagggttag ggatccc
//
LOCUS       DATNDD010000269         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1232165, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000269 DATNDD010000000
VERSION     DATNDD010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232165"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VN647_06395"
     CDS             complement(<1..1150)
                     /locus_tag="VN647_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache and HAMP domain-containing protein"
                     /protein_id="HWG96696.1"
                     /translation="MALWSRSHSLQQKIIAAIVMVGLLPLTLLLALIYVEERRALRES
                     TGANFKEVAVEAARRIEIHITQGMNEAQQLATTPFLRTAVSEANRTYEGKDAQSISEI
                     IKDWQQRWGQRDKRSEFPHFVNRIVTNYLIRWHEIRKSDYVGILVTDGQGALVVSSIP
                     QVEYSYAKTAWWQAVIKGGSQQPYVSEIAFDPSFGTHVVIVAAPILDDQRRAVIGAVT
                     ILLRRDTLFQSIAEGSFGATGHAMLFASDGGGVVICPVLAPEAHSIDAELLGTMGAPK
                     PGWAVAADDSHGNKRALIGFAPVRIAGQLATGSLGGKRWITLVRQDAAETFAPLGELM
                     TKMLLFGSVMLIGLGGIGVVVARRIARPIRLLHDGVQQIGSGRLEQRVE"
     gene            complement(1268..2185)
                     /gene="secF"
                     /locus_tag="VN647_06400"
     CDS             complement(1268..2185)
                     /gene="secF"
                     /locus_tag="VN647_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWG96697.1"
                     /translation="MLEILGKTNIDFIGKRKYSFLFSGIMVLLGLIALVQIARGAANL
                     GIDFAGGTAVQLKFEQTVRIDEARKALETNGLSDAELQEFGQDNKLLIRVKASTTIEE
                     KTAERVVGIFAKEFPNNKFVVDSTTEIGPTIGKKLQEDALVAVVISFAGIILYIAARF
                     ELRFGVAAALATFHDVLAVVGAFYILDKEITLLIVTALLTLAGYSLTDTVVVFDRIRE
                     NLKLRRRESEEATINSAINQVLSRTIVTSLTVVLVLVPLTMAGGEVLHDFSLALLWGV
                     IFGTYSSVFVASPLLLLWPGAPGRLLKRG"
     gene            complement(2245..>2548)
                     /locus_tag="VN647_06405"
     CDS             complement(2245..>2548)
                     /locus_tag="VN647_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWG96698.1"
                     /translation="MGVDSNVLIFERIREEIRGGKAVRSAIDAGYDKAFLTIVDSHVT
                     TLITGVALFLFGTGPIKGFAVTLCLGIAINLFTALVGTKVIFDLLYHPQRVEALSI"
BASE COUNT          583 a          759 c          668 g          538 t
ORIGIN      
        1 actccacccg ctgctccaaa cgaccgctcc caatctgctg cactccgtca tgcaagagtc
       61 gaatcggccg cgcgatccgg cgggcaacga cgactccgat tcctccaagc cctatcagca
      121 tcaccgagcc gaacaacagc atcttggtca tcagctcacc cagcggagcg aacgtttccg
      181 ctgcatcctg acgcacaagg gtaatccacc gttttccgcc gagactgccc gtcgccagtt
      241 gaccggcaat ccgcaccggt gcgaacccga tcagggctcg cttattgccg tgcgaatcgt
      301 cggcagccac tgcccatcct ggtttcggag cacccatcgt accgagcaat tcggcatcga
      361 tcgagtgggc ctcgggagca aggactgggc aaatcaccac accacccccg tccgaggcga
      421 acaacatggc atggcctgtc gcgccgaagg agccctcagc gatggactgg aacaatgtgt
      481 cacgccgaag gaggatcgtc acagccccga tcacagcccg acgttgatca tccaggatcg
      541 gcgcagccac gatgaccacg tgagtcccaa acgatgggtc gaaggcgatt tcactcacat
      601 aaggctgctg gctgccgcct tttatcacag cctgccacca ggcggtcttg gcataggaat
      661 attccacttg aggaattgaa ctgacgacca aggcgccctg tccatcggtg accaaaatgc
      721 cgacatagtc tgattttcga atttcgtgcc atcggatcaa gtagttggtc acgatccggt
      781 tgacgaagtg cggaaattcg cttcgcttat cgcgctgccc ccaccgctgt tgccagtcct
      841 tgatgatttc ggaaatactt tgagcgtctt tgccctcgta cgttcggttg gcctccgaga
      901 cggcggtgcg aaggaagggt gtcgtcgcga gttgttgggc ttcgttcata ccttgagtaa
      961 tgtgtatttc gatccgacgg gcggcttcga cggccacttc tttgaaattc gcccccgtcg
     1021 actctcgcaa ggcacgtcgc tcttcgacat agatgagggc gagcaggagt gtaagcggca
     1081 ggagaccgac catcacgatc gctgcgatga ttttctgctg gaggctgtga gatcggctcc
     1141 agagggccat aagggtgctc cgctaatgat gcggaaacct gtgaagggtg acgcgttctg
     1201 aatgatcttc ggaaacgaac gacgaacaac gcttcacgag cgacgcccgc tctattccac
     1261 cattcgctca gccgcgtttc aagagtcgac ccggcgctcc gggccacaac aataagagag
     1321 ggctggcaac gaataccgat gaataggtgc cgaaaatcac accccagagc aacgcaagtg
     1381 agaaatcgtg cagaacttct cctcctgcca tggtcaatgg gaccaggacg agcacaacgg
     1441 tcaaactggt gacgatcgtg cggctcaaca cctgattgat ggcggaattg atcgttgctt
     1501 cctcgctttc tcgacgccgc aacttcagat tttccctgat ccggtcgaac acgacgacgg
     1561 tgtcggttaa ggaatagccg gccagcgtca agagcgccgt cacgatcagg agcgtaattt
     1621 ctttgtccag gatatagaaa gccccgacca cggccaagac gtcatgaaac gtcgccaatg
     1681 cagccgcgac gccgaaccga agctcgaatc gcgccgcgat gtacaaaata ataccggcaa
     1741 atgaaatgac gacggccacg agcgcgtctt cctgaagctt cttaccgatg gtcggcccga
     1801 tctcggtcgt tgagtccacc acgaacttgt tgttcgggaa ctctttggcg aagattccca
     1861 ccacgcgttc cgcggtcttt tcttcaatcg tcgtggaagc tttcacccgg atgaggagtt
     1921 tgttatcttg tccgaattcc tgcagctctg catcactcag accgttggtt tcgagagcct
     1981 ttcgcgcctc atcaatccgg accgtctgct cgaacttgag ttggacagcc gtgcccccgg
     2041 caaagtcgat gcccagattc gccgcacctc gtgcgatctg gaccagtgcg atgagcccga
     2101 gcaagaccat gatccctgaa aagagaaagg agtacttgcg tttgcctatg aaatcaatgt
     2161 tggtcttccc gagaatctct aacatgcgag ctcccttttg ataaccctga gtctccccct
     2221 ggtggctgat tgccgacttc atagtcagat gctcaacgct tcgactcttt gtggatgata
     2281 caacagatca aaaatcactt ttgtcccgac caacgccgtg aagaggttaa tggcaatgcc
     2341 caggcacaat gtcacggcaa aacctttaat cggcccagtt ccgaataaaa acagagccac
     2401 cccggtgatc aacgtcgtga cgtgcgagtc gacgatcgtc aggaatgctt tgtcgtaacc
     2461 cgcgtcgatg gccgatcgta cggcctttcc accgcggatt tcttcacgaa tgcgctcgaa
     2521 aatcaagacg ttcgaatcga cacccatg
//
LOCUS       DATNDD010000270         3542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3758860, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000270 DATNDD010000000
VERSION     DATNDD010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758860"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            523..1815
                     /locus_tag="VN647_06410"
     CDS             523..1815
                     /locus_tag="VN647_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG96699.1"
                     /translation="MKQGAFDLTSLMNRRILVMLPLGFASGLPLALTSGTLQAWLTVE
                     EVDLKTIGIFTMVGLPYTLKFLWAPVMDRVIPPWLGRRRGWMVLTQLCVAVGLVLMAA
                     TNPRIHPGWMAAYALLVAFLAASLDIVFDAYRTETLHSHERGLGAAVWVNGYRIALLA
                     SGAGALVLADHVGWQMTYLAMAAVMLAGLFIVLISPDPTFAAEAPKSMREAVGAPLAE
                     FFGRPHALGFLAVIILYKLGDAFASTLQTAFLIGGLGFSATDVGAVKGLGVFATLLGA
                     FVGGLLMIRSGLVRSLLIFGVFQAVSNLGFVALAVAGKNATALTAVIVVENVTGGMGT
                     AAFVALVMTLCDPRYTATQFALLSSLEALGRVFAGRPSADVVTLVGWTQFFVLTVVVA
                     LPGLWAVWRVRRFLEPEQRTVVRTSLSEPPVTPVSERL"
     gene            complement(1810..2103)
                     /locus_tag="VN647_06415"
     CDS             complement(1810..2103)
                     /locus_tag="VN647_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96700.1"
                     /translation="MFVWSKKLVASLLSIFVLLLGIFLSLGSPDSPSGLKIHTTQWII
                     LNYACLLVWLFVWMIDQARVRGKNVWLWLLPFVLAPLPTLMLFVLFLQRRLSS"
     gene            2235..2606
                     /locus_tag="VN647_06420"
     CDS             2235..2606
                     /locus_tag="VN647_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HWG96701.1"
                     /translation="MSLLRLTVVVVVLAGAVVLALSNPTMDDYLRFVEQELSKAMDRM
                     DRGAPTREQQFIRQVFQSQSRKLLESVVRPNTARQNWGIASRYETQVADTKVIVLGLG
                     GRFIPLQGVEEATLKIGRMAF"
     gene            2884..>3542
                     /locus_tag="VN647_06425"
     CDS             2884..>3542
                     /locus_tag="VN647_06425"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWG96702.1"
                     /translation="MRIDRRFTRRGQNPYEGIMFVKRSSEIRNPDGSTVFKLENIGVP
                     EAWSQLAVDILAQKYFRKAGVPQRDQNGQSIISPDGKPVLGGERDARQVFERMAGCWT
                     HWGKTYGYFKTPEDAESFHDEMCCMLAHQMAAPNSPQWFNTGLHYAYGLSGPAQGHYY
                     VDPETREVLKAVNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTN
                     FS"
BASE COUNT          737 a          919 c         1024 g          862 t
ORIGIN      
        1 cgcgaccgct tgattgctct cgtgcacaac gtactcgcac agcccgcgcc gactcttgca
       61 gtagaaacca gaccacgcgc ggccaacgat accgactggt tcggttgaac atctagcagg
      121 atgctgaaaa agtccgccag cggcgttctc gcatcgctca gaagctcaag gtaccaccgg
      181 gcaagagttg ctcgagcaac tcggggcggg tgggtgaaaa aggatatgcc tcgcctctaa
      241 gacactgggc gctcaccggc tcgcgcccgt ccgcaaacgt gacgctcatt attcttcgcg
      301 tcgcggacct tgctgcggcc ttgctggact gactttttga acagcctgcg ggctcttaac
      361 aacgccttat cagtttctgc accggtcggt gaggccgttc ccgcatgttt tgtgattctg
      421 ctgaacagcc ctgggagggg atggtattct gccttgcggc ggaaaatcgc tccggtatgc
      481 aggtctgact gattcggctc acgctcgtac gagagagtct ctgtgaaaca aggagccttc
      541 gatcttacca gtctcatgaa ccgacgcatc ctcgtgatgc tgccgttggg ttttgcatcc
      601 ggtcttcccc ttgcgctcac gtccgggacc ttacaggcct ggcttacggt cgaagaagtc
      661 gatctcaaga ccatcggcat tttcacgatg gtcggccttc cctataccct caagttcctt
      721 tgggctcccg tgatggatcg tgtgatccct ccctggttag gccggaggcg cggctggatg
      781 gtcctgacac aactctgtgt tgcggtcggc ttggtgctta tggccgcgac caatccgcgg
      841 atccatcccg gatggatggc tgcctatgcc ttgctcgtcg catttctcgc cgcttcgttg
      901 gacattgttt ttgatgctta tcgaacggaa acactccatt ctcatgagcg cggactgggt
      961 gccgccgtgt gggtgaatgg ctatcgaatc gctcttttgg cctccggagc cggcgcactg
     1021 gtccttgccg atcatgtggg atggcagatg acgtacctgg cgatggcagc cgtcatgctg
     1081 gcggggttgt ttattgtcct tatcagccca gaccctacat tcgccgccga agctccgaag
     1141 agtatgaggg aagcagtcgg cgcaccactg gcggagttct ttggacggcc tcacgcgttg
     1201 ggcttcttgg ccgtgatcat tctctataaa ttgggggacg cctttgcgtc gacgcttcag
     1261 acggcctttc tgatcggagg actcggcttt tccgctacgg atgtaggcgc tgtgaagggg
     1321 ttaggggttt ttgcgacgtt gctcggtgcc tttgtgggtg ggcttttgat gatcaggtcg
     1381 ggactggtgc gctccttgtt gatcttcggt gtctttcaag cggtctcaaa cctgggattt
     1441 gtcgcattgg ccgtagccgg caagaatgca actgccctca cggcggtgat tgtagttgaa
     1501 aatgtgacgg gaggaatggg caccgctgcg ttcgtagcgt tggtgatgac gctctgcgat
     1561 ccccggtata ccgccactca gttcgcgctg ctgtcttcgc tggaggcgtt aggaagggtc
     1621 tttgccggtc gcccatcggc agatgttgtg accctggtcg gatggacaca atttttcgtc
     1681 ttgaccgtgg tggtggcttt gcctggtctt tgggctgttt ggcgggtccg gcgctttctt
     1741 gaaccagaac agaggacggt cgttcgcacc tctctctcag agcctccggt cacaccagtg
     1801 tccgagcggc tatgaggaca gtcgccgttg aaggaagagc acgaagagca tcaaggtagg
     1861 taacggagcc agcacaaacg gcaacaacca taaccagaca ttcttgccgc ggactcgcgc
     1921 ttggtcgatc atccagacaa aaagccagac taatagacat gcataattca atatgatcca
     1981 ctgggtcgtg tgaatcttca gaccgctcgg actgtcggga gaacctaggg aaaggaagat
     2041 tcccaacaat agaacgaata tgctcaatag gctggcgacg agtttcttgc tccagacgaa
     2101 cataggctcc tcctgactca agaagaatgt aaacggagtt cggctaagct aattggggcg
     2161 ctcttccttt gtcaaatgag atgaagccag tcggtatggt gttgcgttag aagaaaggtc
     2221 gctgcgagga ccccatgagc ctccttcgtt tgaccgtggt ggttgttgta ctcgccgggg
     2281 cagttgttct cgccttatcg aatccgacca tggacgatta tttacgcttc gtcgaacagg
     2341 aattgagcaa agcgatggac cgaatggacc ggggtgcacc gactcgagaa caacaattta
     2401 ttcggcaggt gtttcagtcg caaagtcgga agcttctcga atccgtcgtg aggccgaata
     2461 ccgcgcggca gaactggggg attgcgagcc ggtatgaaac acaggtggcg gatacgaaag
     2521 tcatcgttct cggtctcggc ggtcgattca ttcccctcca gggtgtcgag gaggcgacgc
     2581 tgaaaatcgg gcgaatggcc ttctaaaaag aaaaaaggcc agggtaattt ggagggaccc
     2641 cgattccatc ggttatttct gtggataacc ccgttgataa gcgatcggaa tcggctatga
     2701 gattcgcgtt gcgactattc acagcctttc ctctcatttc ccaaagatcc acaatacttg
     2761 gtgttgacaa aaatttatga tagctatatg tagctaccca ttgaagttaa acgactccta
     2821 tcatgatgat gacgcctaac caaagcatgg tcacaatttt tcccctctct caggaggtct
     2881 accgtgagaa ttgatcgaag atttacccgt cgcgggcaga atccctacga ggggatcatg
     2941 tttgtgaaac gttcgtcgga gattcggaat cccgacggat ccaccgtatt taagctcgaa
     3001 aatatcggcg ttccggaggc atggtctcag cttgccgtcg acatattggc ccagaaatac
     3061 tttcggaagg ccggagttcc gcagcgcgat cagaatgggc agtcgatcat tagcccggac
     3121 ggcaagccgg tattgggggg cgagcgagac gctcgccaag tcttcgagcg catggccggg
     3181 tgttggactc attggggcaa gacctatggg tattttaaga cgccggaaga tgccgagtct
     3241 tttcacgatg aaatgtgctg catgctggcg caccaaatgg ccgcgccgaa ttctccccag
     3301 tggttcaata caggccttca ttacgcctat gggttatcgg gaccggcaca agggcattat
     3361 tacgtcgatc ccgagactcg cgaagtgttg aaagccgtca acgccttcga acatcctcag
     3421 cctcatgcct gtttcattca atcaatcgag gatgatttgg tgaatgaaaa cgggatcatg
     3481 gatctttggg tccgggaggc gcggttgttc aagtatggtt ccgggaccgg gactaatttt
     3541 tc
//
LOCUS       DATNDD010000271         4893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2861282, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000271 DATNDD010000000
VERSION     DATNDD010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2861282"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..584)
                     /locus_tag="VN647_06430"
     CDS             complement(249..584)
                     /locus_tag="VN647_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWG96703.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFAAHGAVASARIITDKFTGQSR
                     GFGFVEMSSDSEAQAAITALNGSEMGGRTLTVNEARPQEPRTGGGGGRGGFGGGGGRS
                     GGGGKRDRW"
     gene            complement(810..1601)
                     /locus_tag="VN647_06435"
     CDS             complement(810..1601)
                     /locus_tag="VN647_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG96704.1"
                     /translation="MKLHRITALIVRHLYLYRRSLPRIMEIFYWPFLDLVIWGFITLY
                     LARYQSQVPGFVTFFLGALILWDMLFRSQQGITISFLEELWARNLMNLFASPLKPSEF
                     LAATMAMSVMKVMIVSIVMGGCALFFYSYNILVVGVWLMPFVLNLVITGWIIGLFTTS
                     LIMRFGQEAEVLAWSMVFLFQPISCVFYPMEVLPKWLQMIAWMNPAAHIFEGMRSVLS
                     TSESPVGNLAWAVCLNGALLVGVVTWFYRTVAYCKDQGLLVRVGE"
     gene            complement(1598..2008)
                     /locus_tag="VN647_06440"
     CDS             complement(1598..2008)
                     /locus_tag="VN647_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96705.1"
                     /translation="MTDVLIDSVAGFLTGGLALSAAWGLFWSAISLVGLSRRTCGWRI
                     VFMSLVGGSVPLALAIALVWWVGHLERMTAVFAVGLMGMPAVLAGFWLRRMPDGQRAG
                     THLAAGVRHLMGDLLGMHHGCGACHDGHDHRACR"
     gene            complement(2017..2745)
                     /locus_tag="VN647_06445"
     CDS             complement(2017..2745)
                     /locus_tag="VN647_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG96706.1"
                     /translation="MFTSVLKVSDLTKRFGDFTAVNGVSFEIRPGEILGLLGSNGAGK
                     TTIIQMLLGLVTPTSGSIHMFGLDLSTHREEILQQVNFSSTYISMPQSLTVEENLWVV
                     ARLYGVSDIARRVNDIVKKLEMEEFRCKVTRKLSSGQLTRLTLAKAFLTEPRILFLDE
                     PTASLDPDIADKIRSLLKEERRSSGLSILYTSHNMREMEKMSDRIIFLQRGRIVAEGT
                     AQEIIDRFGKADLEEVFLKFAREQ"
     gene            complement(2767..3477)
                     /locus_tag="VN647_06450"
     CDS             complement(2767..3477)
                     /locus_tag="VN647_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWG96707.1"
                     /translation="MSGKSNATLLPAIVIGAIALVGTAIPFTDHPTFCAGCHTIAPAY
                     ESWAKSSHKEITCVACHVRPGVQGWLSDKVLAGARDAAIYVFGTPTDAHDLKARVDSG
                     VCLSCHRHILRVSEIAPRDLPPPVKQVGLVMNHRQHMEAFKARGQDEGCTTCHSGVVH
                     GEPIKGYPTVIPRGHVSADSQPWYPPHPEGSYLRARALSDCFRCHDGKAQHRGKTLDR
                     KCETCHIPEKISGALLFN"
     gene            complement(3461..3979)
                     /gene="mobB"
                     /locus_tag="VN647_06455"
     CDS             complement(3461..3979)
                     /gene="mobB"
                     /locus_tag="VN647_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HWG96708.1"
                     /translation="MPIPIVSFIGRSNSGKTTLIERIIPELVKAGYRVATVKHAGHGF
                     DLDTEGKDSWRHKRAGAGSVVVVSKGSLALFADVSEQLKVEEVRDRFLDASYDLIIAE
                     GWKSEGYPKIIVVRDQLGEIAYSSDGLLAVVTDKPIDLPAPVLHLDDVAGVAALLIKQ
                     FPRRHREHEREV"
     gene            complement(4066..>4893)
                     /locus_tag="VN647_06460"
     CDS             complement(4066..>4893)
                     /locus_tag="VN647_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HWG96709.1"
                     /translation="IAKGTRIRPSEVGMLAILAKSFVCVYQRPRVAILATGDELADLD
                     ERFSEEKIVNSNSYGMAAAVQEAGGVPLLLGIARDTPMALKEKISQGLNADMVVLSGG
                     VSMGDYDFTKVVFRELGAEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFE
                     QLVRPALLKMSGCRSYGRPMIQAIFQERFSKRGDRRHFLRGILTRGEGVFKVRTTGDQ
                     GSGILTSMVKANCLIDVPVEVERLNPGDEVVVQLLSSEAWPAHTEQGHSGTHRLSCC"
BASE COUNT         1187 a         1482 c         1236 g          988 t
ORIGIN      
        1 agaaccgtcg ctcatccggc gaccaaaagt tttccacaac cagcaccgga cttctctgga
       61 atgagaatga aggtaaatcg gccggtccta tcgtcgattg cgcaacattc atgcatcttc
      121 acggttcttg agatgaatca cgcagaccat cagcctcgat ctattatcct ctagttctat
      181 cgaccaaaaa aagaaggggt cgccaccttc catggcaacc ccttcattct gcatattggg
      241 ttaatcaatt accagcgatc gcgctttcca ccgcccccgc tccgacctcc gccaccgccg
      301 aagccaccac gccctccgcc gccgcctgta cgcggttctt ggggacgcgc ttcattgact
      361 gtaagagtcc ggccccccat ttccgacccg ttgagagccg tgattgcagc ttgagcttca
      421 gagtccgagg acatctccac aaagccgaat ccacgggatt ggccggtgaa cttgtccgta
      481 atgatccggg ccgaggcgac tgcgccatgg gccgcgaata agtcactcaa ttgctgctcg
      541 gtcgccgaat aaggcaaccc accgacatag atcttcgaac ccatcggggt tcctccttta
      601 aaataatgac attgtcttga gcacgaggaa cggggaagga gcgggccgaa ggcgcgatcg
      661 atgcagcgac ttaggtctga cttccgacaa gattcccgga caaggcaaca tcggcattgc
      721 gggtctcctc tgatagacac tcaggtcgcc gagacccagc acgacatgag cctgccgtac
      781 actagcacag agctctctgg aatacaacat cactccccga ctcgaaccag aagcccttgg
      841 tccttacaat aagcaaccgt ccgatagaac catgtgacga cgccgacaag caaagctcca
      901 ttcaggcaaa ccgcccaggc aaggttccca acgggtgatt cgcttgtact caacaccgac
      961 cgcatgccct caaagatatg tgccgccgga ttcatccatg caatcatctg cagccacttg
     1021 ggcaacactt ccatgggata gaaaacacag gagatcggct ggaagagaaa taccatgctc
     1081 caggcgagca cttcggcctc ttgtccaaac ctcatgatga gtgaggtcgt aaacaagccg
     1141 ataatccagc cggtaatgac caaattgagc acaaacggca tcagccatac cccgacgacc
     1201 aggatgttat acgaataaaa aaagagtgca cagcccccca tgacaatcga gacgatcatc
     1261 actttcatca cgctcatggc catcgtggcg gccaaaaact cgctgggttt cagcgggctg
     1321 gcgaacagat tcatcaagtt acgcgcccag agctcttcga gaaaggaaat cgtgattccc
     1381 tgttgcgatc ggaacaacat gtcccacagg atcaacgcgc ccaaaaaaaa ggtgacgaac
     1441 cccgggacct ggctttggta tcgggcgaga tagagcgtga taaaccccca gatcacgagg
     1501 tccaggaaag gccagtaaaa gatttccata atgcgaggca aactgcgtcg gtagagatac
     1561 aaatgcctga cgatcaatgc cgtgatgcgg tgcagtttca tcgacacgcc ctatgatcat
     1621 gtccatcgtg acaggcccca catccgtgat gcatgccgag aaggtccccc atgagatgtc
     1681 ggaccccagc ggccaggtgg gtcccggccc gttgtccatc gggcatccgc cgcaaccaaa
     1741 aacctgccag caccgccggc atccccataa gtcccacggc aaacacagcc gtcatgcgtt
     1801 caaggtggcc cacccaccac acaagagcga tcgcgagggc aagcggcaca gatcctccca
     1861 ctaaactcat gaagacaatg cgccagccgc acgtacgtct gctcagaccg accaggctga
     1921 tcgccgacca aaagagaccc caggccgcac tgagtgcaag ccctccggta aggaatccgg
     1981 cgacactatc gatcaagacg tccgtcacga tgacgactac tgctcccgtg caaatttcaa
     2041 gaatacctct tccagatcgg ctttcccgaa tcggtcgata atttcctgcg ccgttccttc
     2101 cgccacgatg cgtccgcgct gaagaaaaat gatgcggtcc gacatttttt ccatttcgcg
     2161 catgttatgt gatgtgtaaa ggatactcag gccggatgat cgccgttctt ctttcagcaa
     2221 cgatctgatc ttgtcggcga tatcaggatc caaactcgcc gtcggctcgt caagaaagag
     2281 gatgcgcggt tcggtaagaa atgctttcgc caatgtgagc ctcgtcagct gaccagacga
     2341 taattttcgg gtaaccttgc agcggaactc ctccatctcc agctttttga cgatatcatt
     2401 tacacgccga gcgatatccg aaaccccgta aaggcgagcc acgacccata aattttcttc
     2461 taccgtgagc gactggggca tggagatgta ggtagacgaa aagttgacct gctgcagaat
     2521 ctcctcacga tgggtcgaga gatccagacc gaacatgtga atagagccgg acgtaggggt
     2581 tacgagtccc aagagcatct ggatgatggt cgtcttcccg gctccgttcg accccaagag
     2641 tcccaggatt tctccaggcc tgatctcaaa ggatacccca ttgaccgccg taaagtcgcc
     2701 gaaccgcttg gtcagatccg acactttcag tactgaggta aacatcacgc tgacggattc
     2761 ggtcgattaa ttgaacagca aggcgccgct gatcttctcc gggatatgac aggtctcgca
     2821 tttccggtcc aacgttttcc cgcgatgctg tgccttccca tcgtggcagc ggaaacagtc
     2881 actgagtgcc cttgcgcgaa ggtaggagcc ttccggatgc gggggatacc aaggttggct
     2941 gtcggccgat acgtgtccgc gtggaatgac agtgggatac cccttgatgg gttcaccatg
     3001 cacgacgccg gaatgacaag tcgtgcaccc ttcatcctgt ccccgagcct tgaacgcctc
     3061 catatgctgg cgatggttca tgaccagccc cacctgtttg actggtggag ggagatcgcg
     3121 tggggctatc tcggacacac gaaggatatg gcgatggcaa ctgaggcaaa cgccggaatc
     3181 cacccgtgct ttgagatcat gcgcatcggt cggcgttcca aatacgtaga tagctgcatc
     3241 tctggctccc gccaggacct tgtcggataa ccagccttga acaccaggcc gtacgtgaca
     3301 cgcgacacaa gtgatttcct tatgggaaga tttggcccag ctctcataag caggggcgat
     3361 cgtgtgacat cctgcgcaga atgtcgggtg atcggtaaac gggatcgccg ttccaaccag
     3421 agctatcgct ccgatcacga tggccggcaa cagtgtagca ttagacttcc cgctcatgct
     3481 cccggtgtct acgggggaac tgtttgatca agagagcagc aacaccggcc acatcgtcca
     3541 gatgcagcac aggcgcagga agatcgatcg gcttgtcggt gaccaccgcc aggaggccgt
     3601 cggaagagta cgcaatttca ccgagctgat cacgaacgac gatgatcttc ggataccctt
     3661 cgcttttcca cccttcggcg atgatgaggt cgtaggaggc gtccaggaaa cgatcgcgca
     3721 cttcctccac cttcagctgt tcggagacat cggcaaacaa ggcgaggctt cccttcgaga
     3781 ccaccacaac actgccggca ccggcacgtt tatggcgcca actatccttc ccttccgtat
     3841 caagatcaaa cccgtggccg gcatgtttga ccgtcgccac acggtatccg gccttgacca
     3901 attcggggat gatgcgttca atcaaggttg ttttaccgct gttcgaccgg ccgataaacg
     3961 aaacgatggg aatcggcata gaaatcatcc gtcactgatc atcgatcacc ggacaaaaga
     4021 gaatccttct ctcatgacca ccgacgattg accgttgact cctttctagc aacaagagag
     4081 gcggtgtgta ccggaatgcc cttgctcggt atgagcgggc catgcctcac tactcaataa
     4141 ttgaaccacc acctcatcac cgggattgag ccgctcaact tctaccggca catcgatgag
     4201 gcaattcgcc ttgaccatgg acgtgaggat accagagccc tggtctccag tcgttcgaac
     4261 cttgaacacg ccttctcccc tggtgagaat gccgcgcagg aaatgccgcc ggtcgccccg
     4321 cttggaaaat cgttcctgga aaatagcctg aatcatcgga cgtccatagc tccgacaccc
     4381 gctcattttt agtaacgcag gtcgcaccaa ttgctcgaag gttaccatcg acgaaaccgg
     4441 gttgcccgga agcccgaacg cgagcttgcc ctggatcttc ccgaaggcca gcggctgacc
     4501 gggtcggatg gcgagtttcc aaaaattcat ctcggcgcca agttcccgga acaccacttt
     4561 ggtaaaatca tagtcgccca tcgagactcc gccggacaac accaccatgt cggcattcaa
     4621 cccctgggat atcttttctt tgagcgccat cggcgtatca cgcgcgatac cgagcaatag
     4681 cggaacgcct cccgcttcct gcacggcagc ggccatccca tagctgttcg aattgacgat
     4741 cttctcctcg ctgaaccgct cgtccaagtc agccaactcg tcgccggtcg caaggatcgc
     4801 cacccgaggc cgttgataga cgcacacgaa cgacttcgcc aggatcgcca acatcccaac
     4861 ctcgctggga cggattctgg tcccttttgc gat
//
LOCUS       DATNDD010000272         6390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1918632, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000272 DATNDD010000000
VERSION     DATNDD010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918632"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..642)
                     /locus_tag="VN647_06465"
     CDS             complement(19..642)
                     /locus_tag="VN647_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWG96710.1"
                     /translation="MGRAQAFIGALILAMGISAVGCAGPSEAIIAEANSADNDFVLGP
                     EDVLDITVWRSEELTQKGIVVRPDGYISMPLIGEVDASWRTAKELAAHIAERLKEYKE
                     KPTVHVKVKEVNSYYVFVLGDVAKPGKFQLKSHTTVLQAIAMAGGFTPYAAKNNMHVR
                     RTVVGPPGHSKELKIPARYDDLLSGDGDIKDFVLKTGDMVVVPGRAW"
     gene            complement(714..995)
                     /locus_tag="VN647_06470"
     CDS             complement(714..995)
                     /locus_tag="VN647_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWG96711.1"
                     /translation="MRKPRFTRDQIKAMVSELDAGLMAGEVSRRYGISISTLYRWRAR
                     FAHEEQPDDKERLRSLEDEHRRLKKQFAELTLDYATLRAALIRDVRGDC"
     gene            complement(1059..1634)
                     /locus_tag="VN647_06475"
     CDS             complement(1059..1634)
                     /locus_tag="VN647_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HWG96712.1"
                     /translation="MVATYKDERAARYAQVMVPEVEQVTVSKTDRQWYALQTYSRHEK
                     QVRDRLLAVGIEPLLPLGKQCRQWSDRKVWTTLPLFSGYCFANFALAGSLAVLQTPGV
                     VRIVGTLRPEPIPDEEIAVLQQVSSVNPMMEPCDYLIEGAWVEVIQGPLVGLRGHLVR
                     RASHHGLVIRASLLQRAALIHIGSDEVAPVQ"
     gene            2233..2916
                     /locus_tag="VN647_06480"
     CDS             2233..2916
                     /locus_tag="VN647_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG96713.1"
                     /translation="MPNAAMPTLTIAILSSQCLVWLGLQKILESSTTVQMVVHPYPGR
                     TSDLLRTECRPDVFIVDLETERDALSTLKQIRESAPISKIVLLCGLEDKDRTREAFAA
                     GVDGIILKVQPPEVVLAVIEALYASAKPPSALAHDRAGGMGLGPSFTKQVESAPPPPA
                     WPDALTEREREVIRLVGQGLSNKDIAYKLSISDSTVRHHMTNIFDKVGVPNRQKLLVH
                     AHHFRTTPV"
     gene            3159..3234
                     /locus_tag="VN647_06485"
     tRNA            3159..3234
                     /locus_tag="VN647_06485"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3192..3194,aa:Phe,seq:gaa)
     gene            complement(4399..4674)
                     /locus_tag="VN647_06490"
     CDS             complement(4399..4674)
                     /locus_tag="VN647_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWG96714.1"
                     /translation="MTLLTIFEAAKFLRLTKRTLYQRVDIPRVRYGHRVMFVKEDLEN
                     WVRGSYEGGVLKDIDEQGVLPKPVDGRPGKVYHRNPLFVLLRHKGMR"
     gene            5044..5244
                     /locus_tag="VN647_06495"
     CDS             5044..5244
                     /locus_tag="VN647_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96715.1"
                     /translation="MFHHTEKVGGGGTKPGIVWAGTLESRGVTHMGPQAAEAAFMPAP
                     SAVPYWMSADKEWLQAGRTSTQ"
     gene            complement(5329..5949)
                     /locus_tag="VN647_06500"
     CDS             complement(5329..5949)
                     /locus_tag="VN647_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96716.1"
                     /translation="MCLKEGGIAKIFNDLSNPLCIPFALTTHQWVLDEMGGARSNSKK
                     AIGRVEQDVDIGGMYKSHELIPLDKADPIHALMRLSDTIGLWTKELGRTVKVQILPDT
                     VAKRQGSSDNRIKCQHRLWLRGAYSDWKYLTPVPEAGGTFVFVNGVQDDYLFQVQIQH
                     YIKDSNGVVQKLHVDKSTKSDNVITGDLVKAYVTVQWHTTTLQRDN"
BASE COUNT         1466 a         1797 c         1595 g         1532 t
ORIGIN      
        1 ttgccacacc atgccttgtt accatgctct gccgggcacc acgaccatat cgccggtctt
       61 cagcacaaag tctttgatgt ccccgtctcc ggacaacaga tcatcatacc gtgcaggaat
      121 cttgagttct ttggagtggc cgggtggccc gaccacagta cggcgtacgt gcatgttgtt
      181 cttcgcagca tagggggtga accctccggc catcgcgatg gcttgcagga ccgtcgtgtg
      241 cgacttgagt tgaaacttgc ccggctttgc gacgtctccc aagacaaaga catagtagct
      301 gttgacctct ttgactttga cgtgtactgt cggcttctcc ttgtactctt tcagccgctc
      361 ggcaatgtgt gcggccagct ctttggccgt tcgccaactc gcatcgactt ctccgatcag
      421 cggcatcgag atatagccgt ccggcctgac cacgatccct ttctgagtga gttcctcact
      481 gcgccagacg gtaatgtcaa gcacgtcttc gggccccaat acaaaatcat tatcggcgct
      541 gttggcttcg gcaatgattg cctccgaggg gcctgcgcac ccgactgcgc ttatccccat
      601 cgcaagaatc agcgcgccga taaacgcttg ggccctgccc atttccttcc ttttcatggc
      661 attcgctgaa gatctacagg cacaactgtc acttctttga caacaattac cgtctaacag
      721 tcgcccctca catctctgat caaggcagct cggagcgtcg cataatcgag cgtgagctcg
      781 gcaaattgct ttttcagccg tcgatgttca tcctctaatg agcgtaaccg ttctttatca
      841 tccggttgtt cctcatgggc aaacctcgcc cgccaccggt agagagtgct gatggaaatc
      901 ccataccgac gtgagacttc tccggccatc agaccggcat ctagttccga gaccatggct
      961 tttatctgat ctcgggtaaa acggggtttg cgcatgactg ccgaatcccc tgtcgtcatc
     1021 ccacattcgt ggaaagagcg gaggggaagg aggaaggttc actgcacagg cgcaacctcg
     1081 tcggatccta tgtggatgag cgcggctcgc tgcaggaggg aggcgcggat caccaatcca
     1141 tggtgactcg cccgtcgtac gaggtgaccg cgaaggccga caagcggtcc ttgtatcact
     1201 tctacccatg cgccttcaat aagatagtcg cacggttcca tcatggggtt gaccgacgaa
     1261 acttgctgta gaacggcaat ttcttcatcc ggaataggtt cgggtctcaa cgtgccgaca
     1321 atgcgaacga cgcccggtgt ctgtagaacg gcaagactcc ccgctaacgc aaagttggca
     1381 aaacaatacc cgctaaatag cggaagcgtc gtccacactt tgcggtcgga ccattggcga
     1441 cattgcttgc ctaacggaag caagggttca atccccactg ccagcagccg gtcgcggacc
     1501 tgtttctcat ggcgagaata ggtctggagg gcataccact ggcgatcagt tttcgatact
     1561 gtaacctgtt ctacctccgg taccattacc tgtgcatacc gtgcggcgcg ctcgtcttta
     1621 taagtagcga ccatagcttc gccccgtgag tcccagtcca ttttcataaa aatacggcta
     1681 ttcagccgta attagcaggc actaaaggcc ctatgtagta ccaggggccg atgtcactaa
     1741 gccataagat agatgtcgag ttttcgtgta gccattaccg gcacatttgt ctgaacgatt
     1801 ggtcgctgga accactacat ctgatcacgt gctggaacag gattgcttct attcgaatga
     1861 ctctagcctc catttaccca taacaatccg attcactccc ttcgcctgta atagcgccta
     1921 tcaataagtt tccctcatga gtaggttgag tgagaactgt acgaccgaca agaaaaatga
     1981 gaatacgata gcgtattcaa cgaattggat atgtgtgatg ggttcaacca tgcataccct
     2041 actaatgggc agtatcattt gtacctattc agaggagaaa atttttgact gtttgcgaaa
     2101 ggtagtcagg aagattccag aataatgggg tagccagagg tcggagttct tccatgtgga
     2161 cagggagcga ccgaaaccag agaaacaggt ttcaatagta acgagtcttc agtcttggac
     2221 cagggaggca gtatgcccaa cgccgcgatg ccgacgctca cgatcgcgat tcttagcagt
     2281 caatgtctcg tgtggttggg cttgcagaaa attctcgaga gcagcacgac tgtccagatg
     2341 gttgtgcacc cctaccccgg aagaacatct gacctgctcc gcaccgagtg ccgccccgac
     2401 gtgttcatcg tggatctgga aaccgaacgc gacgccctta gtaccctcaa acagattcgg
     2461 gaatccgccc cgatcagtaa gatcgtgttg ctgtgcgggc tcgaggacaa ggaccgcaca
     2521 cgcgaggcgt ttgccgccgg ggtcgacggc atcatcctca aggtgcaacc gccggaagtc
     2581 gtgctcgcgg tgatcgaggc gctgtatgcc tctgcgaaac ccccgtccgc cctggcacat
     2641 gatagagcgg gaggaatggg cctcgggccc tctttcacga agcaggtgga atcagcccca
     2701 ccgccgccgg cgtggcccga cgccttgacc gagcgggagc gagaggtcat ccgattggtg
     2761 gggcagggac tctcgaacaa agacattgcc tacaagttgt cgattagcga cagtacggtc
     2821 cggcatcaca tgacgaacat tttcgacaag gtcggtgtgc ccaatcgaca gaagctcctg
     2881 gtccatgcgc atcatttccg caccacaccg gtctaactac cattggctgc cacctactac
     2941 ctcttctcca ttccaggtcg tgaaccttct cccctgagtg ctcattgcgt tgcatgcgaa
     3001 acgcgacgaa actcatacga gcagtacgcc tacaagaagg atgcgtgcgc gcaaaagatg
     3061 tggaagtcag acgtgcgacc ttggtaatat gcgcgggata tcggtgggtc ctgcgaatat
     3121 gagaaccatc cagcgctgaa aaatcgaatc cctcctgtgc cgaggtagct cagttggcag
     3181 agcacagccc tgaaaaggct ggtgtcgaca gttcgattct gtccctcggc accactttcc
     3241 gcatacttag cgatttcatc tcaggagcaa gtggcacctg agtggcagta agtggcacct
     3301 gaacatcgtt ttctccgctt agaactgcct tcgtaaacgc ttccagccgt gtcactgcat
     3361 cccgcaatcg cgcgttccaa ttgtgaatgt agtacttcgc cgaaccaggt agcggctcac
     3421 cttttaaagt ctggatcaca gcgtaatcca cgccacattg tgtgagccag gtcgtgaagg
     3481 tatgacgaag atcgtggaag tgcagatcgt gaacccctgc ccgctcacac gttcgcatcc
     3541 atgtatgctt gaaggaattc ccatctttcc agtgatggaa aaatcggcca ccgattctca
     3601 gagtttttcc gaacaacgcc tcgtaagcca gactgttaag gggaaccatt ttaggcaccc
     3661 ctttgattct cgtctggccg ggcgacggca cgagccacca cccgtctccc cgttgcaaca
     3721 gccactcttc atgaatctct atcagcttgt tttctcgaag gccgatctgc agcgcggcca
     3781 tcgcaagccg ccaaacgttc ggtgaggcgg catcacggat tttgagcagc tcccagccct
     3841 cgacaatccg ctcgcgcttc acatattcag gcacaggcaa acgtttgagc cgattcttgt
     3901 ccagatggtc catatcaacg gctaaattca gcaccgccat aagagttgcg cactctcgct
     3961 caatcgtccc tggagccaca tgtcgtttct tatcctctgg gccgatcaag acagagcgac
     4021 gcttttccag ataagccaat ccatcttcaa ggcagatacc agagagcttc ttgtggccaa
     4081 aatgcggaat aagatggtgc gtcagaacgg tttgattccg cccatcgttc ttcggtcgct
     4141 tggctttaag atattgaagg taaatcggaa caaattcagc aaacgtcagg gttgcaggct
     4201 gcccagtact agcgatagga gcttgcgttg tttcgtgttc ggaccaattc tcatgaatcg
     4261 cagtaatgat ttcgagagca ctctctcgtt gacgatcgag cgtcaagtta tgcccaagta
     4321 ccggtcgata gcgtcttcca cgataccaat aggtcagaat cgatcgtgtt cccttgggag
     4381 tattgatgcg ccgaatattc atcgcatgcc tttatgacga agcaacacaa acaaagggtt
     4441 ccgatggtag accttgccag gtcttccgtc aacaggcttc ggcagtactc cttgctcatc
     4501 gatgtctttc aacacaccgc cctcatatga gcctctgacc caattttcaa gatcttcttt
     4561 tacaaacatg acacgatgcc catagcgaac ccgagggata tcgactcgct gatagagggt
     4621 ccgttttgta agcctcaaaa acttcgctgc ctcaaaaatt gttaagagtg tcatgggttc
     4681 ccctgcttag ttctcgccca ttgaacatac gtgcttctca atctctctgt tatcggggta
     4741 gtcttgcgac accacgtttt cgcgggatgt gcccgcgtca acgccgccgt gaatcgatga
     4801 ctgggctccc agtggtgacg gtccaacgct gaagagcagc ggatcaggcc ttcccggttt
     4861 cacttcaaat caggtcattc tctaggcctg accgccgcga gcgcgctttt ccctcctcct
     4921 tgtctgtccc accccgggtt tctgtgcggc agcctttcac gtcatgcttt acacattcgg
     4981 ttgagtgacc cgaccgacat cttcgcacac ctctcgtgag tggcacttat gagagggttc
     5041 agaatgtttc accataccga aaaggtaggg ggaggcggca caaaaccggg gatcgtatgg
     5101 gcgggtacgc ttgagtcccg aggagtcact cacatgggtc cgcaggcggc tgaggctgca
     5161 ttcatgccgg ctccctctgc cgttccctac tggatgtcag cagacaagga atggctccaa
     5221 gccggaagaa ccagcaccca atgataggta gtgaccgcaa tgagaaccac gattggtagt
     5281 ctaacgtgta acgcatctat tcattacacc gatagagaac ggatggactt aattatcacg
     5341 ttgcaacgtc gtcgtatgcc attggaccgt cacataagct ttgaccaagt cgcccgtaat
     5401 cacgttatcc gacttggtac ttttgtcgac atggagtttt tgcaccaccc cattgctgtc
     5461 cttgatgtag tgttgaatct gcacttgaaa caaatagtca tcttgaacac cgttaacaaa
     5521 cacaaacgtg cctcctgcct caggaacagg cgtcagatat ttccagtctg aataggctcc
     5581 ccggagccac agacgatgct gacacttgat cctgttgtcg ctacttccct gtctcttcgc
     5641 aaccgtgtcc ggcaggattt gcactttcac ggttcgcccc agctcttttg tccatagtcc
     5701 gatggtatct gatagtctca tcaatgcgtg gataggatcg gccttgtcta aaggaatgag
     5761 ctcatgcgac ttgtacatgc ctccaatgtc gacatcctgt tccacccggc cgatggcttt
     5821 cttgctgttt gatcgcgcac cgcccatttc atcaagcacc cattgatggg tcgttaatgc
     5881 aaagggaatg caaagggggt tgctcaagtc attgaagatt ttggcaatcc ctccttcctt
     5941 caagcacata gcccacttcc tctttgctgg acttcgtatc aattcatctc attttcatct
     6001 cattttggcc tagtctatcg ctccaggtaa caccgtagga acacctagca gaaggcacgc
     6061 ggtatcaatc ggcagacgga ggtgcctctt ggagtcctct agcgcatagt atttcatctc
     6121 cagcgctctc ctcccagaac gaacaccctg ggcatttcgc aaagcgcacg taggccaaaa
     6181 gggaagtcgc cgcataagga agcctcgata gtgggttacg actcacatca ttattcacca
     6241 gaagggggac taagccccct ccagatgcgg gattgggttt cgtggatatt ggggcatgat
     6301 ggaactgtca tgcagcggct catggatcat caccctaatc ttcacactgg aaggaatcga
     6361 caatggtcat ggtcatacaa tctcaagaac
//
LOCUS       DATNDD010000273         8037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3560864, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000273 DATNDD010000000
VERSION     DATNDD010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3560864"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VN647_06505"
     CDS             complement(<1..105)
                     /locus_tag="VN647_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWG96717.1"
                     /translation="MSMKLYVGGLPYSATESQLTTLFAEHGTVESARVI"
     gene            complement(363..659)
                     /locus_tag="VN647_06510"
     CDS             complement(363..659)
                     /locus_tag="VN647_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96718.1"
                     /translation="MSRINLDPLLTFPDGSHLVISTQFSAGEDFSCALYSAVVGNDDC
                     VTFRVISNHFTASTCMSVQESAYNYALRLYPDAAAVMKKPPYLIWHGPRSSRVQ"
     gene            complement(1511..2983)
                     /locus_tag="VN647_06515"
     CDS             complement(1511..2983)
                     /locus_tag="VN647_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWG96719.1"
                     /translation="MGLFTAWPAWGLTAGDLYRSGKEATALVIGIDDEAHALSFGSGV
                     FISQDGLLLTNAHVIHEATRVVVYGAADSLTPTPEIVAVDPDLDLAALRIPYAHPVPY
                     LSLASEPAEDGMAAVTIGYPRLPDVLQMGLTIHPTVFPVTVSGEAMGQSRIAHRPLPF
                     LHITGLMNAGSSGGPLVAAASGHILGLVVHTVPYLETAKNTQGESIGRVMLRAGLSYC
                     IPSSRIREWLDADHLLSGGVNEPPGQTQPAMPKPTASDGFVTTAHLLQLMADVIRKDP
                     DLLDMAIQHYESALLLESDRPDILHDLGRALASKGNVDDAWARYEAALARLPDDLELL
                     TDAGDLRRQQRQFDKAETFYRQALQRDSCYSRALLGLGNTMAEQGDSQEAGRAFQAVA
                     HCPSASSLAAYRLGIELERHGESTGALTLWEHALEQLSNQVPAEQGLITRIRHRANQL
                     RNRLQRPSAQTTDDFHLSMAGAGGIKDNHPINTSRSEQTQ"
     gene            complement(3233..3652)
                     /locus_tag="VN647_06520"
     CDS             complement(3233..3652)
                     /locus_tag="VN647_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWG96720.1"
                     /translation="MAKTLIEPTSTSADDLRAMLLERRSAVQQEIHELLTQYREHYTA
                     THNHSVLDLEDMSLRDSTAEQQLSLLEARNRTRMMLDTALRRLDDGEYGLCEDCGAKI
                     GAGRLRALPFAKRCLSCQHQAEVLEKIARQEDRRDDA"
     gene            4014..4673
                     /locus_tag="VN647_06525"
     CDS             4014..4673
                     /locus_tag="VN647_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWG96721.1"
                     /translation="MRLTVATYNVHRCIGWDGRYGRERILAVLQELQADLIALQEVDE
                     ENTLQWLAVHLSMTAIAGPNLRRHGAYGNGLLTTCKLGEFRLVDLSFRGHEPRGAIDV
                     DVVCGNERVQVIATHLGLRPSERRWQVRQLLTRFGTRHCILLGDINEWFLWGRPLRWI
                     TNMFGHTPSLRSFPSPWPIFPLDRIWIRPPGSVIEIRTHKTALSSVASDHLPVKAIIE
                     L"
     gene            complement(4809..5459)
                     /locus_tag="VN647_06530"
     CDS             complement(4809..5459)
                     /locus_tag="VN647_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HWG96722.1"
                     /translation="MPTATLQRKYEPRSYDLHDLHGISDRTLAMHFKLYEGYVKETNN
                     LTARIHQLVEDGKVDQEEMPVYSELKRRYGFEYNGMVLHEYYFDNLMAHGSPDPGAHS
                     SFRRAAESSFGTYEAWKGDFIGVGKMRGVGWAVCYLNPYNGTLTNHWITLHETGNVAG
                     FAPVLVMDVWEHAFILDYKPSERVSYIEAFFSNINWKAVEHRLERTIAPTSISALT"
     gene            complement(5528..5815)
                     /locus_tag="VN647_06535"
     CDS             complement(5528..5815)
                     /locus_tag="VN647_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96723.1"
                     /translation="MVPTYWEALRIGWTILWRGVGSVVLLLMLVNGLIIIGTPELSRT
                     SPSWGAAFYPFALASLIGAFGIMPWLVKCLCTISYPGFRLRLVHESSASQT"
     gene            complement(5835..6065)
                     /locus_tag="VN647_06540"
     CDS             complement(5835..6065)
                     /locus_tag="VN647_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96724.1"
                     /translation="MLKAFAINLLLVAYLVSASMQAAILVRPHEASPPTDPSPGPTDP
                     TPKPSGPIPPPQPPPPKPNPVYPSPSPGLDST"
     gene            complement(6111..6485)
                     /locus_tag="VN647_06545"
     CDS             complement(6111..6485)
                     /locus_tag="VN647_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HWG96725.1"
                     /translation="MEMKTLSQQHQNCLNACFSCAQTCEACANDMIGMEHHDHNDLMT
                     RCIRLCRDCADICVLAAKFISRSSQQAESLCRLCAEICDECAEVCERHAPQHALCGPC
                     AEECRRCADLCREMVGTVVPSA"
     gene            complement(6550..7074)
                     /locus_tag="VN647_06550"
     CDS             complement(6550..7074)
                     /locus_tag="VN647_06550"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HWG96726.1"
                     /translation="MSKPKRTDADRLIRRYYEGENTGQQGAFPEDTLASVPHVDPRSS
                     DGMTPDVTLSGGDVDATRSGTDDGKEAVGGSTPTPDQDVVDELGEAAGITYHEAEPLR
                     FGDKVADRDAQRWELNPASSEGYQERLRELTGSGRPTPTSPRTKQPKQAARTKQSTPS
                     RQTVGARKGQKKNR"
     gene            complement(7150..7602)
                     /locus_tag="VN647_06555"
     CDS             complement(7150..7602)
                     /locus_tag="VN647_06555"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HWG96727.1"
                     /translation="MAPTGKHLSFVLIFLVGAAGCDATRELVKAPFEATTALSNGTTQ
                     ATNELLEPLKEFTSSTTPGSMYNDPFKAKRRFQVFIAYTFNNVQRDIAQGQGEYLTSL
                     ATLAGIPEGRRPQFFAMLQDHYGTIYSADTALIESLQWLTAAAWPERN"
     gene            complement(7714..>8037)
                     /locus_tag="VN647_06560"
     CDS             complement(7714..>8037)
                     /locus_tag="VN647_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWG96728.1"
                     /translation="GWPLAVILKYADAVTAGQSTETEWMAILLWPSLAYLVVQFIESW
                     VLTPWLQSHSTDMGAATILIVVFLGGAVGGLFGLIFAIPAATCIKILIEEYIRPRWLQ
                     WVREA"
BASE COUNT         1786 a         2194 c         2252 g         1805 t
ORIGIN      
        1 tatcacgcgg gccgattcga cggtgccgtg ctcggcaaac agggtggtga gttgagattc
       61 agtcgccgaa tatggcaagc cgccaacata cagtttcata ctcatgtggt ctcccttgta
      121 ggaatgacat tgtccatggc gcgagggact tcggagagga aggagagggc caaagacata
      181 gttggtgaac ggctcaggcc aggcttccga tcagattctc ggcaggaaca acatcggtga
      241 taggccgttt gatacgcatt tcatacgagg ccctccgcat gaaacgttca cattgtacag
      301 cgagcaagat gaaatagata tcagccgtcg cccgtcaggc attcagtcat cagtacggta
      361 aatcactgta cccttgacga gcgcggccca tgccaaatca agtagggtgg ttttttcatg
      421 accgcggccg catctggata gagccggagt gcgtaattgt aggcggactc ttgaacgctc
      481 atgcaagtcg aagcggtaaa gtggttcgag atcacgcgga atgttacgca gtcatcgttc
      541 ccgaccacag cgctatacaa ggcgcaggag aaatcttcac ccgctgaaaa ttgtgtactg
      601 atcacaagat gcgatccatc cggaaaggtc agaagcggat ctaagttgat gcgactcatc
      661 ggcgcgctcc ttcggctagt cgggcagact ttacgtatgc ggatgcatag ggcatgacga
      721 aactcgcaaa cacttgccct cgaacggcac actttcagtg cgacgaccgt acacagcgct
      781 tgcttgcaga acccatttcc gcgaatccat agccggacga tttcccacta tgtttgtgtc
      841 ggacgacatg ggcgcgccgc aacaccatag gttccaaata ggtcgtggag cttgataatc
      901 ggaagtggtg tccgtgagcc cacccgcata caaaagatgt tttctcaagt catcctccat
      961 ttgtactgcg atcgcgcctg agttcgagtg ggaattgggt tacccgtgat ctattcatcg
     1021 gttctcacta ttaaaagttt tattcactac ctgtgcgaat cagttcagcc tgagatggcg
     1081 ttacttctgg acaagaccgt tcagagaact ccggccccgg agcggcggag actgaggaat
     1141 cggtcacgct gagaagaagt aggaagcgta agagatgaga cagtcaccga agcttcgcag
     1201 catcaccttg ggcgcaatgg gcatgagagt caagcactat tgtggatcgg ggcaacaggc
     1261 acgttcgaaa gctggactga cggtgaggca aagtgccttg atcgcacatc gtgaaataga
     1321 aatggactgg ttcaaggcga gagacccccc ccaatggcta aactcattct atcgcacgtg
     1381 cgccacgcca gaatccactt ttagccacag aaaagccaca atctcaagag aaatcaccaa
     1441 tcagaaactc cttcgggcaa tgcaacacgt tggatatggt gccgaagacc atcctatgca
     1501 gccgtatgtc ctattgagtt tgttcgctcc ggctggtgtt gatcgggtgg ttgtccttga
     1561 tgccgccggc tccggccatg ctcaagtgga aatcatcggt ggtctgcgcg gagggccgct
     1621 ggagacgatt cctcagttgg ttcgcgcgat gacgtatgcg tgtgatcagg ccttgttcag
     1681 ccggaacctg atttgaaagt tgctccagcg catgttccca cagggtcaag gctccggtcg
     1741 actcgccgtg gcgttcgagt tcaatgccga gtcggtaggc tgccagtgag gaagcagacg
     1801 gacaatgggc gacggcttga aacgcccgcc cggcctcttg agaatcaccc tgttcagcca
     1861 tcgtgttccc gaggcccagc agggctcgcg aatagcagga gtctcgctgc aaggcctgtc
     1921 ggtagaaggt ttccgccttg tcgaattggc gctgctgacg gcgaagatcg ccggcgtcgg
     1981 tcaagagttc gagatcatcg ggcaggcgtg ccagagccgc ttcatatctc gcccatgcat
     2041 catcgacgtt gcccttggaa gccaatgccc ggccgagatc atgcaaaata tccggccgat
     2101 cggattccag caacaacgcc gattcatagt gttggatcgc catatcgagg aggtcgggat
     2161 ctttccgaat gacgtcggcc atcaactgta gtaaatgagc cgtggtgacg aatccgtcgg
     2221 acgcggtcgg cttcggcatg gccggttggg tttgaccggg tggttcatta accccgcccg
     2281 ataagagatg atcggcgtcg agccattcgc gaattctcga ggagggaatg caataactca
     2341 agcccgctcg caacatgacc cgtccgatcg actcgccctg cgtattttta gccgtctcca
     2401 gatacggaac ggtatggacg acgagtccga gaatatgccc ggacgcggcc gcgacgagcg
     2461 gaccgccgct gcttcccgca ttcatgagcc cggtgatgtg cagaaagggg agcggtctgt
     2521 gggcgatgcg cgactgtccc atcgcttctc cgctcactgt gacagggaat actgtcggat
     2581 ggatcgtcaa tcccatctga agcacatccg gcaagcgagg atatccgatc gtcacggcgg
     2641 ccatgccgtc ttctgccggc tcgctcgcca acgacaaata cgggacggga tgagcgtatg
     2701 ggatgcgtag cgccgcaagg tcgagatcag gatccaccgc gacgatctca ggagtcggtg
     2761 tcaggctatc agccgcccca tagaccacaa cacgagtggc ttcatggatg acatgcgcgt
     2821 tggtaagcag caagccgtct tgagagatga acacgccgct gccgaaggaa agggcatggg
     2881 cttcatcatc gataccgatg acgagcgctg tcgcctcctt gccggaacgg tacagatcgc
     2941 ctgccgtcag cccccaggcg ggccatgcag tgaacagccc cacgcagact aggatggtac
     3001 ggacggcaag gagactatgg agtcctgcga gtgtgatagt gtccctcggt gtgacgggat
     3061 gcactgtaca ctacgcaggg atggggaaga aagccagtag aatctcataa acagaattgc
     3121 agcgaaacta ggcgaacccc cagtctatga agctaccgtc ccacctcaca ctcacaccta
     3181 cgcgcaaatc ttcgagccat gatgctgagg acaagcgcgc gcggcacaag gtctacgcgt
     3241 cgtcccgacg atcttcttgt cgcgcaatct tctccagcac ttcggcctga tgttggcacg
     3301 acaagcaacg ttttgcgaag gggagggctc tcaggcgtcc cgcgcctatc ttggcaccgc
     3361 aatcctcgca caatccgtat tctccatcat ccagtcggcg gagtgcagta tcgagcatca
     3421 tgcgagtgcg gttacgcgct tccagcaacg ataattgttg ttcggccgtg ctgtcgcgta
     3481 aggacatatc ttcaaggtcg aggacggagt gattgtgggt tgcagtatag tgctcccgat
     3541 actgggtcaa aagttcatgg atctcttgtt gcacggcaga gcgtctctca agcagcattg
     3601 ctcgaaggtc gtccgccgat gtggaagtag gttcaatgag tgtcttggcc atgatcaagc
     3661 tcctccccta attgaagtgt ggatcagtgc agcacctttg cttgatctgc aaaaatgagg
     3721 ccgccgcttt gtatcattcg catgcttcca aaatgaaaaa ttccaggatc aattccgaca
     3781 atgcatcact tttttcaaga gcggaccgat gatgtatttt tttgccaatg caatgtccat
     3841 aaaggatgaa ggacaagttg tcctttcaag gagcatattc taataatact cgttacctca
     3901 ttaagagtga gggcggtacg acctcttgac ccgtgagatc ttggatacca tgggtattac
     3961 gattgcacct ctaccggatc actcggcaat tttccagggt tggtatcgta accatgcgcc
     4021 tcacggtggc cacgtataat gtacaccgct gcattggatg ggatgggcgg tatggacggg
     4081 aacgtatcct tgcagtgctg caggaattgc aagccgatct cattgcacta caggaagtgg
     4141 atgaggaaaa caccttgcaa tggttggccg ttcacttgag catgaccgcg atcgccggac
     4201 cgaatttacg gcgccacggc gcctacggca acggcctgct gacgacgtgc aagttgggag
     4261 aattccggtt agtcgatctc agtttccgtg gccatgaacc ccgcggagcc atcgatgtcg
     4321 atgtggtgtg cggcaacgag agggttcagg tcatcgcgac ccatctgggt ttacgtccga
     4381 gcgaaagacg ctggcaagtg cgccaactcc tcacacggtt cggcacgcgc cattgcattc
     4441 ttttggggga catcaacgaa tggtttttat ggggacgacc tcttcgatgg atcaccaata
     4501 tgtttggaca cacgccctct ctccgatcat ttccctctcc ctggcctatt ttccccctcg
     4561 accgtatctg gatcaggcct ccgggcagcg tcatcgaaat tcgaactcat aagacagccc
     4621 tctcaagcgt ggcttcagat catttgcctg tgaaggccat catcgaattg taaccgagga
     4681 tgctgcaaaa gtcctccagc gtcgttctcg catcgctcaa ggtctcaacg taccaaccgc
     4741 gtacgcctcg acctctcgct cgctgcggcc ttgctggaag aactttttca ccatcctcgg
     4801 gaattatgtc acgtgagcgc cgaaatggat gtcggcgcga tcgtgcgctc cagacgatgt
     4861 tcaacggcct tccagttgat attggaaaaa aaggcctcaa tgtacgagac gcgttccgaa
     4921 ggtttgtaat ccaggataaa ggcatgttcc cacacatcca tgaccagcac aggggcgaag
     4981 ccggccacat tgccggtttc atgcagcgtg atccaatgat tggtgagtgt gccgttgtag
     5041 ggattgagat aacatacggc ccagccgact ccccgcattt tccccacgcc gatgaaatcg
     5101 cctttccagg cttcatacgt gccgaagctg ctttcggccg cgcgtctgaa cgaggagtgc
     5161 gcccccggat cgggcgatcc gtgggccatg aggttatcga aatagtattc gtgcaggacc
     5221 atgccgttgt attcgaaccc gtatcgccgt ttgagctccg agtagaccgg catctcttcc
     5281 tgatcgactt ttccgtcctc caccagttga tgaattcgcg ccgtgagatt attcgtctcc
     5341 ttgacgtaac cttcatagag tttgaaatgc atggcgagcg tccggtctga aatgccgtga
     5401 aggtcgtgga gatcatagga tcgtggttca tactttcgct gcagtgttgc ggtgggcatt
     5461 gtgtccctct ccttcatgta aatggttcat gttcagacaa tcgcatattc atacaatgtg
     5521 aagcgattca tgtctgagaa gctgaggact catgcacgag tcgcagacga aaccccgggt
     5581 acgaaatcgt acagaggcac ttcacgagcc atggcatgat gccgaaggct ccgatcaagc
     5641 ttgccagcgc gaacggatag aaagccgctc cccaggaagg gctcgtgcgg gagagttcgg
     5701 gcgtgccgat gatgatcagt ccgttgacaa gcatcagcaa cagcacaacg cttcccactc
     5761 cccgccagag aatagtccat ccgatccgga gcgcttccca atatgtcggt accatgtgaa
     5821 acatcctttc aacttcaggt tgaatcaaga cccggcgagg gcgacggata caccggattg
     5881 ggcttgggag gaggaggctg aggaggcgga atcggcccgg acggtttggg agtcgggtcg
     5941 gtcggtcctg gacttgggtc cgtcggcggg ctcgcttcat gcggacggac gagaattgca
     6001 gcctgcatcg acgcggatac taaataggca acgagcaaca ggttgatcgc aaaagccttc
     6061 aacatgcacc tcttcctcag aaagccgccg gttgtccggc tcttatcaag tcatgccgag
     6121 gggacgacgg tcccgaccat ttcacgacag agatccgcgc accgacggca ctcctccgcg
     6181 catggaccgc acaaggcatg ttgcggcgca tggcgctcgc acacttcagc acactcgtcg
     6241 caaatctccg cgcagagccg gcacagagac tcggcctgct gtgaggagcg gctgatgaat
     6301 tttgcggcga ggacgcatat gtcggcgcaa tcgcggcaca gtctgataca acgtgtcatc
     6361 aaatcattat ggtcgtgatg ttccatgccg atcatatcgt tcgcgcaggc ctcgcaggtt
     6421 tgggcgcatg agaaacaggc gttcaggcaa ttctgatgct gttgcgagag agtcttcatc
     6481 tccataatag tcggctcctt tcctgtccat acatcgactc attaaaaaac atcgactcat
     6541 caaaacatgt cagcggtttt tcttttgccc ctttctggcg ccgaccgtct ggcggctggg
     6601 tgtgctttgt ttggtcctgg cggcttgctt cggttgtttc gtccggggac ttgtcggtgt
     6661 cggtctgccg gagccggtga gttcccgcaa tcgttcctga tatccttcgg aagaggcggg
     6721 attcagctcc catcgttgcg catcgcgatc ggccacttta tcgccgaacc gcaagggctc
     6781 ggcttcatga taggtgatgc ctgccgcctc ccctaattcg tcaaccacgt cctgatccgg
     6841 ggttggagtc gaaccgccca cagcctcttt accgtcgtcc gtcccgctcc gcgtcgcatc
     6901 gacatcgccg ccggacagtg ttacgtcggg cgtcatccca tcggaggagc gcggatcgac
     6961 atgcggaaca gatgccagcg tatcttccgg gaaagcccct tgttgtcccg tgttctctcc
     7021 ctcatagtac cggcggatga ggcgatcggc gtccgtgcgt ttcggttttg acatggtcta
     7081 tccttcaatg tgtggttcgt tcataagata taagattatg tgaacggcga ccgggttgaa
     7141 attgcgttct cagttcctct caggccaagc cgcggctgtt aaccactgca aggattcgat
     7201 cagcgcagta tctgcggaat agatagtgcc atagtgatct tgcagcatgg caaaaaattg
     7261 ggggcgtcga ccttccggga tgcccgcaag ggttgcgagc gacgtcagat attccccttg
     7321 gccttgggcg atgtctcgct ggacgttatt gaacgtatag gcaataaata cttggaatcg
     7381 acgcttcgcc ttgaatggat cgttatacat cgaaccggga gtggtgctgg aagtaaactc
     7441 cttgagcggc tcgaggagtt catttgtagc ttgcgtcgtc ccattggaca gcgccgtcgt
     7501 cgcttcaaaa ggcgctttga ccaattctct cgttgcgtcg catcccgcag ctccaaccag
     7561 aaatatcagt acaaacgaaa gatgctttcc tgttggtgcc atggcaagct cctttcttcc
     7621 gataccgctg ttcgcccgac accttgattg tatgcttaat gtacgcgggc tcctcagaag
     7681 gctgtactcg gtcattccct agattgagga ggtctaagcc tcgcggaccc attgcagcca
     7741 ccgcggcctg atatattcct cgatcagaat tttgatacag gtggcggcgg gaatcgcaaa
     7801 gatcagcccg aacagccctc ctacggctcc gcccaagaag accacgatga gaatcgtcgc
     7861 ggcgcccatg tctgtggagt ggctttgcag ccagggagtc aagacccagc tttcgatgaa
     7921 ttgcacgacc aggtaggcca ggctcggcca gagcaaaatg gccatccatt ctgtctcggt
     7981 ggattgaccg gcggtcacag catcggcgta cttcagtatg acggctaagg gccatcc
//
LOCUS       DATNDD010000274         4849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_854934, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000274 DATNDD010000000
VERSION     DATNDD010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854934"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VN647_06565"
     CDS             complement(<1..1230)
                     /locus_tag="VN647_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWG96729.1"
                     /translation="MDVNRMTIKLQEALQSASAHAQRRSHQGIDVEHMLLALIDQEGG
                     TAPALLEGAGLALPAVRQAVEQALAKLPQVQGSGAAPGQMHIATRLTQVLNHAEDEQK
                     SLKDDFLSVEHVLLAMVQEGGVLKKLGLTRDRLLSGLQQVRGNQRVTSQDPESTYQSL
                     VKYGRDLTQLAGQGKLDPVIGRDDEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AIRIVKGDVPESLKHKKLFALDMGSLVAGAKFRGEFEERLKAVLKEIQSSQGQILLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGELHLIGATTLDEYRKHIEKDAALERRFQT
                     VLVDQPSVENTISILRGLKERYEVHHGVRIKDSALVAAAKLSHRYISDRFLPDKAIDL
                     VDEAAARLRTEIDSLPAE"
     gene            1407..2237
                     /locus_tag="VN647_06570"
     CDS             1407..2237
                     /locus_tag="VN647_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96730.1"
                     /translation="MVNLVSATLQLYRHVLYATGRSFARSWVTVVALIVFAILFLGTS
                     RIAAPFGIGGGFILGIVNALLVGATLRLIEQSLSGARSIQLRDVTESFGHYFWDVIGV
                     GFVLWLPTMLLDMGMQANPYGQFLLSAFLLLVFILLNPAPEVIYQVRHDSTLNVFKTS
                     YEFVLEHWVEWFLPFAILILPVVLSPSGLQEFFTLSGRVGRGAGLDFFQILMLPLTAI
                     GGWLSYVGLDSEGQGIILLLLTPPMAMAILLFRGHLFASLHGSSRRQRLFAHQFGTRQ
                     "
     gene            2420..3895
                     /locus_tag="VN647_06575"
     CDS             2420..3895
                     /locus_tag="VN647_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWG96731.1"
                     /translation="MQLSIFWRLVMTSLVIITVMGGVNIYALFQLRQLTAMSTQMASY
                     HYPAAESAKRLLGSLYAQLNSEKKYLATNDHTFLTNLTEEVDEFQHSLQLLRGQESSP
                     QGLKLLKETDELLKEQLKLFRNELQTAKGKLRQVTPEYENHRDVLMDRMSSSIQGYID
                     LHEARVSVGVTESRASAAQAEAVTEQLVLVALVFGLGLAGIASYTILRPLRELQGHIK
                     QIGQGNFGASLQIKAPAELRELVDTVNWMGRKLQEIDNMKTEFLAHVSHELRTPMASI
                     QEGTHLLLDEIPGPLVQEQRTTLRIMADSSKRLIHLISTILDLSKMEAGMMEYRIVPV
                     DLQRIGEISINKVRLLADSKHVQLVLESVGERAWVKADASRLEQVLDNLLSNALKFSP
                     EGGVVKVQMKPDPQAGVLEAAVSDTGPGIAPEDLPHVFERFVQGRMKAKHTAGSGLGL
                     ALAKKVVEAHGGRIWIESEKGKGATVRFILRLAKPETVGQA"
     gene            4111..4677
                     /locus_tag="VN647_06580"
     CDS             4111..4677
                     /locus_tag="VN647_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96732.1"
                     /translation="MRGLLGLYESREIAEKYFEKVLTVAPKSHLAASSKAWLELLQQP
                     TVPGHKSWPEAVFRAPVLAETNTSLALTADRLVRDLLDRELLIQQLRASKGDDSEVVG
                     ALQRELADRDHKIESLLSKKDSVKAPADPASIQSLQKQLVERDRKIEELSTQLEALKR
                     IDQEMREKVRPMRPPLPTVPVPGPETTP"
BASE COUNT         1048 a         1268 c         1384 g         1149 t
ORIGIN      
        1 ctcggccgga agactgtcaa tttcggtcct gagccgcgcg gcggcttcat cgaccaagtc
       61 gatggcttta tccggaagaa accgatcgga aatgtaccga tgcgacaatt tcgccgccgc
      121 gaccagcgcg ctgtccttga tccgcacgcc atggtggacc tcatagcgct ccttgaggcc
      181 gcgcaagatg gaaatggtat tctccacgga cggttgatcg accaagacgg tctgaaagcg
      241 gcgttccaag gcagcgtctt tttcgatgtg tttccgatat tcgtcgagcg tcgtggcgcc
      301 gatgagatgc aattcacctc tcgccagcat cggcttcaac agattggccg catccatcgc
      361 gccttcagcg gcgcctgcac cgacgaccgt atgcaactcg tcgatgaaca gcagaatctg
      421 gccttgggaa gattgaatct ccttgagcac ggctttgagt cgctcttcga actcgccgcg
      481 aaacttggcc ccggcgacga gcgagcccat gtccaacgcg aagagcttct tgtgtttgag
      541 actctcaggg acatcccctt tgacgatacg gatcgccagc ccttccacga tggcggtttt
      601 tccgactccc ggttcgccga tgagcaccgg gttgttcttg gtccggcggg ataggatttg
      661 aatcacacgt ctgatttcgt cgtcacgacc gatgacgggg tcgagcttgc cttgcccggc
      721 caattgagtc agatcacgcc cgtatttcac cagcgattga taggtacttt caggatcctg
      781 actggtgacg cgctgattgc cgcgcacctg ttgcaatccg gacaagaggc ggtccctggt
      841 gagtcccagt ttcttgagca cccctccttc ctgaaccatg gccaacaaca catgctcgac
      901 actgagaaaa tcgtccttga gcgatttctg ttcgtcctcc gcatgattga ggacctgagt
      961 caggcgggtg gcaatgtgca tttgaccggg agcggcgccg gagccttgca cttgcggcag
     1021 ctttgccaag gcttgttcga ccgcctgccg aaccgcaggc agggcaagac cggccccctc
     1081 aagcagagcc ggagccgttc cgccttcctg gtcgatcagc gccaacagca tgtgctcgac
     1141 atcgatgccc tgatggcttc ttcgctgcgc atgggcggag gcggactgaa gcgcctcttg
     1201 caatttgatc gtcattcggt tcacgtccat ggtgactcac ctgtaaaaag atactcgtga
     1261 aaagatacgt ttatgagcca tctgataatc atcgggtcgc gagaagtcaa ccaagaatcc
     1321 atagaatcca tagaatccgt tttttcgttt ccgggtgcga ctagtccttg acctcttgct
     1381 caatcccgat tagggtacgc accggcatgg tcaacctcgt ttcagccacg cttcaactgt
     1441 atcggcatgt cctgtatgcg acggggcgtt cgttcgcgcg aagctgggtg actgtggtgg
     1501 ctctcatcgt attcgccatt ctgtttttgg gaacgtcacg aatcgccgct ccgttcggaa
     1561 taggaggcgg gttcatactc ggcatcgtca acgccttgtt ggtgggcgcg accctccggc
     1621 tgatcgagca gtctctcagt ggggcgagat ccattcaact cagggatgtg accgagagct
     1681 ttgggcatta tttctgggac gtgatcggcg tgggattcgt gctgtggctg ccgacgatgt
     1741 tgctcgatat ggggatgcaa gcgaacccct atgggcagtt tctgttgtcg gcgtttctgc
     1801 ttctggtctt catcctcttg aatccggcgc cggaggtgat ttatcaagtc cgtcatgatt
     1861 caacgctcaa cgtgtttaag acctcctatg agtttgtgtt ggagcattgg gtcgagtggt
     1921 tcctcccctt cgcgatcctc atcttgccgg tagtgctttc gccgagcggg ctgcaggagt
     1981 tttttaccct ttccggccgt gtcggtcgag gcgcgggcct cgacttcttc caaatcctca
     2041 tgctgccgct cactgcgatc ggaggatggt tatcctacgt gggcttggat tctgaagggc
     2101 aaggaatcat cctgttactc ctgactccgc cgatggccat ggcgattttg ctgttccgcg
     2161 gccacctttt tgcgtcgctc catggctctt ccagacggca acgtttattt gctcaccagt
     2221 ttggcaccag gcagtagggc cgaattcagg ataggctgtc accgagattt ggttcattac
     2281 ataggtacca gtaccagctt tgagacgctt cttgggttct tgatgggtcc tggcttgtga
     2341 tcggaactga aatagtgtat gggttagcct acacttgagc ttgttccttc acgatgtggt
     2401 ccggggacgg tactgcctca tgcaactttc aatcttttgg cgattggtga tgacctctct
     2461 cgtcattatt acagtcatgg gaggagtcaa tatttacgca ctctttcagc ttaggcaatt
     2521 gacggcgatg agtacccaga tggcgtctta ccattatccg gccgctgaat ccgcgaagag
     2581 gctgttgggt tcgctctatg cccaattgaa cagtgaaaag aaataccttg cgaccaacga
     2641 tcacaccttt ttgacgaatc tgactgaaga ggtcgatgag tttcagcaca gtctccaact
     2701 gttacggggc caggaaagct cccctcaggg gctgaaactg ctcaaagaaa cggacgaatt
     2761 gctcaaggag cagctgaagc tgtttcggaa tgaattgcag accgcgaagg gaaagctccg
     2821 gcaggttact ccagagtatg aaaaccaccg agatgtcctg atggatcgga tgtcctcttc
     2881 gattcagggc tatatcgatc tgcacgaggc tcgagtgagt gtcggggtga cggaatcccg
     2941 agccagcgcc gcccaagcag aggccgtcac agaacaactc gtcttggtgg cgttggtgtt
     3001 cggcttgggg cttgccggca ttgccagcta tacgatttta cgtccgctcc gagagctgca
     3061 agggcatatc aaacagatcg ggcaaggaaa tttcggggca tcccttcaga tcaaagctcc
     3121 tgcggagtta cgcgagctcg tcgataccgt gaattggatg gggagaaagc ttcaggaaat
     3181 cgacaatatg aaaacggagt tcctcgcgca tgtgtctcac gaattgcgca ccccgatggc
     3241 ctccattcaa gaaggcacac atctcctcct tgatgagatt ccgggtccgc tcgtacagga
     3301 acaacgaacg acccttcgga ttatggccga cagcagtaag cggctgatcc atctgatctc
     3361 cacgattctg gatttatcaa aaatggaagc cgggatgatg gagtaccgca tagttccggt
     3421 ggatcttcaa cggatcgggg aaatctccat caacaaggtt cgtctgttgg cagactccaa
     3481 acacgtgcaa cttgtgctcg aaagcgtcgg cgagcgggcg tgggtcaagg cggacgcttc
     3541 ccgtctggaa caggtgctcg acaatttact gtcaaacgct ctgaaattca gtcctgaggg
     3601 aggtgtggtg aaggttcaga tgaaacccga cccacaagcc ggagtccttg aggcggctgt
     3661 ttcggatacg ggacctggga tcgctccaga ggatctgccg catgtgttcg aacgttttgt
     3721 tcaaggccgc atgaaggcga agcatacggc ggggagtgga ttgggtttgg cattggcgaa
     3781 gaaggttgtc gaagctcacg gcggaagaat ctggatcgaa agtgagaagg gcaagggagc
     3841 gactgtacgg tttatcctac ggttggcaaa accagaaacg gtagggcaag cgtgacaaga
     3901 aaagcactgt ctctgacaag agcccccatc atcagttcta tcatcttatt attgataggc
     3961 tgtgcagctt ggataacgcc accacccggc tctcgtccct atttttccgc agagcctcga
     4021 gaagtcaaag tttttcaatc gcttgcaaag aagcaagaga gtgtagcctc aaaatgtggc
     4081 gaatcgagct cctgcgacca catctacttt atgcgcggat tgctcggact ctatgaaagt
     4141 cgcgaaattg cggagaaata ttttgaaaag gttttaacgg ttgctcccaa aagccacctg
     4201 gccgcgtcaa gcaaagcctg gcttgaattg ttacaacaac caaccgttcc tgggcacaaa
     4261 tcgtggccgg aagccgtatt tcgagccccg gtccttgcag agaccaatac gtcattggcg
     4321 ttgacggctg atcgtctggt gcgagacttg cttgaccggg agttgctcat ccaacaactt
     4381 cgtgcctcca agggcgacga ttccgaggtg gttggggcct tgcagcgcga gttggcggat
     4441 cgagatcaca agatcgagag cctgttgtcg aagaaagact cggtcaaggc gccggccgat
     4501 ccggcgtcga ttcaaagtct gcagaaacag ttggtcgagc gggacagaaa gattgaggag
     4561 ctttccacgc aactcgaggc gttgaaacga atcgatcagg agatgcggga aaaggtgcga
     4621 ccgatgcgtc cgccgttgcc caccgttccg gttccgggcc ccgagacgac accctaacgt
     4681 atgagagaag ggattatatg gagcaagaaa acattctcgt agtggacgac gatgaggggc
     4741 tgttgcattt gttgaaaatg cggttgtccg cgatgggttt ttcggtcacc ccttgtacga
     4801 cggggcatga tgcggtcggt gaggcgaaga agacgatgtt caacctggc
//
LOCUS       DATNDD010000275         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4120757, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000275 DATNDD010000000
VERSION     DATNDD010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4120757"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VN647_06585"
     CDS             complement(<1..303)
                     /locus_tag="VN647_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HWG96733.1"
                     /translation="MAREIIDPSDAGRCGDFVAMSQKAVFVDKDGTLIENRPFNRSPE
                     HIEWLPGTMEGLRLLHRSGYALIVVSNQGEIAHGRFTVEDLLREELALRAELAKFEV"
     gene            464..862
                     /locus_tag="VN647_06590"
     CDS             464..862
                     /locus_tag="VN647_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96734.1"
                     /translation="MKSIQKIIGIVLCGVVLSVGASSIGLAEHSPSSGDVMKTDSLTD
                     AQKYNFQSDDDKQRNVNDGKSHASGAKTIKGELFRVDDANYFVKTKDGAEVRLHTDRT
                     TNMRGKIRKGDQIEATVNDQNHALSIQAIR"
     gene            978..1505
                     /locus_tag="VN647_06595"
     CDS             978..1505
                     /locus_tag="VN647_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG96735.1"
                     /translation="MLTVIPRRRSGIFGESTNDEAYSVLPGEDRLVKDVMTHEVATAT
                     DSITAKEAIDVVRSRDVPILVVYRRSEPIVALTEYDLAINIITEPRADSVTLYELIKK
                     RMVIRCREDAILADAMDAMLNHCIRHVPVVNRSGGLVGALSLVDAVGAITPDAAALWK
                     RKVRRLWTETPAFKR"
     gene            1630..1980
                     /locus_tag="VN647_06600"
     CDS             1630..1980
                     /locus_tag="VN647_06600"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWG96736.1"
                     /translation="MSSVLRDNRDVLLYRCHFEPRRTLDYIDAKSAHLLDDSSDGFPS
                     GQGQALGDWTHPLDREPAWNAIEEAVRAQRSYLLVYRLIGTEGKDKWVWDEGEAFKTD
                     DGRIAGLEGFLAGI"
BASE COUNT          554 a          553 c          606 g          495 t
ORIGIN      
        1 gacttcgaat tttgcgagtt cggctcgaag cgcaagctcc tcgcgcagca ggtcttccac
       61 cgtgaagcga ccgtgggcga tttctccctg attggacacc acgatcaggg cataacccga
      121 gcggtgcaac aaacgcaatc cctccatcgt cccaggcagc cattcgatgt gttcggggct
      181 gcggttaaac ggacggtttt cgatcaatgt gccgtcttta tccacaaata cggctttttg
      241 actcatggcc acgaagtctc cgcatcggcc cgcatcggat gggtcgataa tctcacgagc
      301 catcgtatcc gcctcccgcg ccctacaggt actaggtgaa accccggcaa cattgccgac
      361 gggggaaagt cccaggttga aaaacttccc ggcaagctta tgtgtaaaaa aggtgtgtac
      421 cgagaggtcg attgccccac aactaaaacg tgaggaggct cgcatgaaat caattcaaaa
      481 aatcatcggg attgtattgt gcggtgtggt gttgtccgta ggggcgtcca gtatagggct
      541 ggccgaacat tcccctagtt ccggagacgt gatgaaaaca gattctttaa ccgatgcgca
      601 aaagtataat tttcaatctg atgatgataa gcaacggaat gtgaacgacg ggaagtccca
      661 tgcaagcggt gcgaagacga tcaaaggcga attgttccgc gtcgatgacg ccaattattt
      721 tgtcaagacc aaggatggag ccgaagttcg tttgcacacc gataggacca ccaatatgag
      781 aggaaaaatt agaaagggtg atcagataga agcgacagtc aacgatcaga atcatgcgct
      841 gtcgattcaa gcgattcgat aaaacatctt cataatatga ccggcaggtg cagcgctgga
      901 ggtggcagag ctatgtttct ttacattagg gattcgccca gtgccattca gcgggatatt
      961 ccgttaagag cgttccaatg cttactgtga tcccccgccg ccgttctgga atttttgggg
     1021 aatccaccaa tgatgaagca tacagtgtgc ttcccggtga agaccgcctt gtgaaagacg
     1081 ttatgactca tgaggtagct accgccacag actccatcac ggccaaagaa gcgattgacg
     1141 tcgttagaag ccgggacgtt cccatcctcg ttgtgtaccg acggagcgag ccgatcgtcg
     1201 cgctcacgga atatgacttg gccatcaata tcatcactga accccgtgct gattcggtca
     1261 ctctctatga gctgatcaag aagcgaatgg tcattcgatg tcgcgaagat gccattttag
     1321 ccgatgcaat ggacgccatg ctcaaccatt gcatccgtca tgttccggtg gtgaatagaa
     1381 gcggaggttt agtcggtgcg ctttcgttgg tggatgcggt aggcgccatc acgccggatg
     1441 ccgcagcact ttggaaaaga aaggtacgac gattatggac tgagacgcct gcgttcaagc
     1501 gatagagcac ctctccgatg tgtcctcgct ctggtatttc cgagttcatt tcttaatatg
     1561 ttccgataat gtcaagccat ggccaaatcc ccctcaatgg taaaagggcg cagctcgaag
     1621 agtcgacctg tgagttcagt attgagggat aacagagatg tcctgttgta cagatgtcac
     1681 tttgaaccgc gtcggacatt ggactacatc gatgctaaaa gcgcccatct gctcgatgat
     1741 tcatccgacg gatttccgtc ggggcagggg caagcgctcg gtgactggac ccatccgctc
     1801 gatcgtgagc cggcatggaa tgccattgag gaggcggtcc gcgcccaacg ttcttacctg
     1861 ctggtgtatc ggctcatcgg tacggaaggc aaagacaaat gggtatggga cgaaggcgaa
     1921 gcgttcaaaa ccgacgatgg acgcatcgcc ggcttggaag gatttctcgc cggtatttga
     1981 tacaacgttt ctctgcccta agtcctatct aggggtccgc ccagtacgag tcccgccgcc
     2041 gacccttata gttagtacat aaaaatcgct aagcatgcgc gacaggaatg cgacaggaat
     2101 gcgataggag ccatacgcaa aacaacgcgg aaggtcaagg ctgaccctat ggaccatgcg
     2161 atcccactcg gagcgcaggc ggtggaagag ggtgtacggt ttagagtg
//
LOCUS       DATNDD010000276         3500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3481775, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000276 DATNDD010000000
VERSION     DATNDD010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481775"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VN647_06605"
     CDS             <1..706
                     /locus_tag="VN647_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HWG96737.1"
                     /translation="RSYLDPGDLPNAADDADWKKISPITIPLSGQVITRPVWPEPTVR
                     ALTVRSVHNGTEIAFLLEWQDNTKNDRLTPGTFRDGVAIGLPLGDAPAFFCMGQLDHY
                     INIWHWKADWQSDIDRRAARGSEKKEGGVRTFEVIPRRVSSVEDLIGGGFSTLTTKER
                     QGRVQGQAVWRDGVWHVMMRRPLVSEEQENEATLVPGRVQTVAFAVWNGENKERNGQK
                     AVAPWFQLAIDPVVKL"
     gene            1027..1464
                     /locus_tag="VN647_06610"
     CDS             1027..1464
                     /locus_tag="VN647_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWG96738.1"
                     /translation="MKRTTWGWAFCLASGLLAVDMAFAQAAMPNGAMTEEEASKLGEE
                     FGIVVGAVDEEIQKELKLQKPQGVAVFEVIGNSRADYAGIKVRSVIKEIDKQEIRNMA
                     DFGRAIKKAMKECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            1495..2823
                     /locus_tag="VN647_06615"
     CDS             1495..2823
                     /locus_tag="VN647_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWG96739.1"
                     /translation="MRGEAEGQNGKVPSGCCIAFVCFLALVGLNGQHDDRAIAQELNG
                     QASSDWVAEIEKVFIRSEDCKQCHERHYEEWKGAREQTPDLKTFGRVDAALLHGTSLE
                     SPVFRTVLGLWKQTNPTPDEQRRCLSCHVPSASAFPQHAEKIAAQVMAGTPKIEGIGC
                     AACHLMNGLEKRLHSPPTFTLQPGGTFYGPYSNPEENLVHPSALSDHFRAASFCASCH
                     FDKVKDAAQKELPGEILQGTICQDCHMESSTGSSTSRRGAMTRAIGRHWFRGVVASGT
                     MLKNRNLQAEWMPRIDIEVAKIGPVVEGTGVVKVGSLPHSFPDGDPVLKQFFLTVRVK
                     DGTGNTLTEETKQFGLPFDKILRGPIPDPFIKGGNTRKVPFSLTLPAGTAAASIEAVL
                     TYSLLPTPEPFLQERYLATLESETGREEARRIIQEYTQRRFLTYRVKSLS"
     gene            3190..>3500
                     /locus_tag="VN647_06620"
     CDS             3190..>3500
                     /locus_tag="VN647_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWG96740.1"
                     /translation="MAKSIHSPAFRASLDLYLNSTMGKDKTQCFRCHAPHVREFSDHA
                     QLFVEQAKAGDPSLDGVACSQCHLIKSLDLAKHPPEPKYEIGSKTLYGPYGDFVQNLA
                     H"
BASE COUNT          781 a          880 c         1064 g          775 t
ORIGIN      
        1 cagatcttat ttggaccctg gtgatctgcc gaatgccgcc gatgatgccg attggaagaa
       61 gatctcaccg atcaccattc ccctgagtgg acaagtgatc acgaggcccg tttggccgga
      121 gcccaccgtg cgtgcattga cggtgcggtc cgttcacaac gggaccgaga tcgcctttct
      181 gctggaatgg caggacaata cgaagaatga tcggctgact cccggcacct tccgggatgg
      241 ggttgcgatc ggcctcccgc tcggagatgc gccggcattt ttctgcatgg ggcagctcga
      301 ccactatatc aacatctggc attggaaggc ggattggcaa agcgacatcg atcgacgggc
      361 ggctcgggga tcggagaaaa aagagggagg ggttcggacc ttcgaagtca tcccgcgccg
      421 agtctcttcg gtggaagatt taatcggcgg gggattcagc acattgacca caaaggagag
      481 gcagggaaga gtgcaaggac aggcggtatg gagggacggt gtttggcatg tcatgatgcg
      541 tcgtcccttg gtgagtgaag agcaagagaa tgaagctacg ctcgttcctg gtcgcgtgca
      601 gaccgttgca tttgccgtgt ggaacggaga aaacaaagag cgaaacggcc agaaggctgt
      661 ggcgccatgg tttcaactgg cgatcgatcc cgtcgtcaaa ctgtagcagg atgttgaaaa
      721 aggccgccgg ctttgttctc gcatcgctca aggcctcgac gtaccgagtc gggacagagc
      781 agctccgact gctcggggtg gacgggtgaa aaaggattac gcctcgccgc taagacactg
      841 ggcgctcaca gactcgcgcc cgtccgcagg cgtgacgctc aattattctt cgcgtcgcgg
      901 accttgctgc ggtcgcgccc ctggagaggc acgtctcggc gtgccgaggt tgggcgggtg
      961 agatcagcgg cctttttgac catcctgcgg gattttcacg agttacgaga tacgaatgaa
     1021 gggcgaatga agaggacgac ttggggatgg gctttttgcc tggcgagcgg actgctagcc
     1081 gtcgacatgg cgttcgcgca ggctgcgatg ccgaatggtg cgatgacgga ggaagaagcg
     1141 tcgaaacttg gagaagagtt tgggattgtt gtcggcgccg tcgatgaaga gattcaaaaa
     1201 gaacttaaac tgcagaaacc ccagggagtt gccgtgttcg aggtcatcgg gaattcacgg
     1261 gcggactatg cgggaatcaa ggttcgatcc gttattaagg aaatcgataa acaggagatc
     1321 cgaaacatgg cggattttgg gcgagcgatc aagaaagcga tgaaagaatg caactttacc
     1381 gttggaacct atgaaccggc agatccaggc gatccggtcg gctggggggt taattttcac
     1441 tttgtcggct gtaagcggga ttgatggtgc gtggtgaggc gtgagaggta agaggtgaga
     1501 ggtgaggcgg agggacagaa cggtaaggtg ccgtcaggct gctgcatagc attcgtctgt
     1561 ttcttggcac tcgtcggtct gaacggtcag catgacgacc gggcaattgc tcaagagttg
     1621 aatggtcagg cttcgtctga ctgggtggcg gaaatcgaaa aagtcttcat ccgttctgaa
     1681 gactgtaagc agtgtcatga gcgtcattac gaagagtgga agggcgccag agagcagacg
     1741 ccggacttga aaaccttcgg tcgtgtggat gccgctcttc ttcacggcac gtcgttggag
     1801 tcacccgtct ttcgaaccgt gttgggtctt tggaaacaga cgaatccgac gcccgatgag
     1861 cagcgccgct gtctatcgtg ccatgtgcct tccgccagcg ctttccccca gcatgccgag
     1921 aagattgcgg cgcaagtaat ggccggcacg ccaaaaattg aagggatcgg ctgtgccgcc
     1981 tgccatttga tgaatgggct ggaaaagagg ttgcactcac cgccgacatt cacattgcag
     2041 cccggcggca cgttctatgg tccttattcc aatcctgagg agaacctcgt ccatccctcc
     2101 gcgttgtcgg atcacttccg agcggccagt ttttgtgcct cttgtcattt cgataaagtg
     2161 aaagatgcgg cccagaagga actgccgggt gagattcttc agggcacgat ttgtcaggat
     2221 tgccatatgg agtcatcgac aggcagttcc acatctagac gcggggcaat gaccagagcc
     2281 atcggacggc attggtttcg tggcgtcgtc gcttccggta ccatgctcaa gaatcggaac
     2341 ttacaggctg agtggatgcc tcggattgat attgaggtgg cgaagatcgg cccggtggtc
     2401 gagggaaccg gggtcgtgaa ggtcggcagt ctcccgcaca gtttccccga tggcgatccg
     2461 gtactgaaac aattttttct gaccgtcagg gtgaaggacg ggacgggcaa cacattaacg
     2521 gaagagacaa aacaatttgg gttgccgttc gacaaaatcc ttcgcggtcc gattcctgat
     2581 cccttcatca agggcggcaa cacgaggaag gtcccatttt cgctgacgct ccctgccggt
     2641 actgccgctg catcgataga ggctgtgctg acctattcgc ttcttcctac gcccgaacct
     2701 ttcttgcagg aacgatacct tgcaactctg gaaagcgaaa caggacggga ggaggcgagg
     2761 aggatcattc aggaatatac gcagcggcgc tttctgactt atcgtgtcaa atcactttcc
     2821 tagcagaatg ttgaaaaagt cctccagcat cgttctcgca tcgctcagag gctcaacgta
     2881 ccgaagcgta cgtctcgcct cttcgctcac tgcggccttg ctggaaggcc cttttgaaca
     2941 ttctgcaggc gattctgatt tggtgcgtga tctctctaac agttcgagag tgaaccggtg
     3001 tctaccatga tggcggttgg gctcagtttt gtcgtaacga ttggtgccgc tgtgttatgt
     3061 gacggcgcgt tcacttccgt cctagcacag aatgcggcaa atcagtcatt gatcgagaag
     3121 acctttcctc attccaccaa atgcaaacgg tgtcacgagc gtgtgtacga ggagtgggaa
     3181 acctccccta tggcgaagtc catccattcc ccggcgttcc gcgcctcgct tgatctttac
     3241 ttgaattcaa cgatgggaaa agacaagacg cagtgttttc gttgccatgc cccgcacgtg
     3301 cgagagtttt cagatcatgc gcagctcttt gtggagcagg cgaaagccgg cgacccttcc
     3361 ttagatggcg tagcttgcag ccagtgtcat ctgatcaagt ctctggacct ggctaagcat
     3421 cccccggaac ccaaatatga gatcgggagc aaaactctct atgggcccta cggtgacttc
     3481 gttcagaacc ttgcacatca
//
LOCUS       DATNDD010000277         3009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_54097, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000277 DATNDD010000000
VERSION     DATNDD010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54097"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VN647_06625"
     CDS             <1..253
                     /locus_tag="VN647_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWG96741.1"
                     /translation="GIGSEVQRPLAIVVIGGLVSSTLLTLIMLPVLYRWLEGRSKDSM
                     HRGEPQTGSTVDGHHTVGEASHANGEPRFTHHPGEIPSM"
     gene            complement(400..702)
                     /locus_tag="VN647_06630"
     CDS             complement(400..702)
                     /locus_tag="VN647_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96742.1"
                     /translation="MNNDQIPIDPAKLQLIRQNLEKFLFSDVQQRYDEQMRTHFSFNE
                     RPQRSEIVFHRKFLDDCKNLNEIMEKSILPLLLANPGKSIIVGEMGGLSIEDREKN"
     gene            complement(1054..1383)
                     /locus_tag="VN647_06635"
     CDS             complement(1054..1383)
                     /locus_tag="VN647_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96743.1"
                     /translation="MYRLISAVICSWLLLAGVAFAGGEQIVQELTGNGSVTTRAFTVE
                     DGWEVRWTATNDFSLSLLDAEGQSLKALGHSIGDAGGATYRATGGTYSLGISSNAHWT
                     VTVVQVP"
     gene            complement(2090..2554)
                     /locus_tag="VN647_06640"
     CDS             complement(2090..2554)
                     /locus_tag="VN647_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96744.1"
                     /translation="MKTRIISMVVAVGLMNERNEAIQVRLPIVVFIIVSTYCSMLVPL
                     AWSHSSSADTSSADIQQGTAYGAGMEAASAAATILYFPFKAAFAIAGGIVGGLAYVFS
                     GFSEPTAKNIRIPSTFGTYVITPEHLTGERPVRFLGVAAEREGGASPAAVHD"
BASE COUNT          693 a          816 c          786 g          714 t
ORIGIN      
        1 cgggatcgga tcggaggtgc agcgtccgtt ggcgatcgtc gtcatcggcg gcctcgtgag
       61 ttcgacattg ttgaccttga tcatgctgcc ggtcctctat cggtggctcg aaggccgctc
      121 gaaggattcg atgcatcgag gggaaccaca aaccggatcg acagtcgatg gacaccacac
      181 agtgggcgaa gcttctcatg ctaacggtga gccacgcttc actcaccacc caggtgagat
      241 accatcgatg tgatgggtga atcgactgag aagggtcggc cttcgtatgg gtaaggtatt
      301 catcaagatg gtcaggaggc cagccgcgcg ggtttcagtt catgcctctt cagttccccc
      361 cttcagtggt tgtgtgctcc ttcggtggac aggagacttt cagtttttct ctctgtcttc
      421 aatgctgagc cctcccatct ctcctacaat gattgatttc cctggattgg cgaggagcaa
      481 cggcaggata cttttttcca ttatctcatt aaggttctta caatcgtcca gaaactttcg
      541 gtggaatacg atctcggagc gctgcggtct ctcgttaaat gaaaaatggg tccgcatctg
      601 ttcatcgtat ctctgctgga catcggagaa gaggaatttt tcaagattct gccgtataag
      661 ttgaagcttc gcaggatcaa taggtatctg gtcattattc ataggtccct cctgtcgcga
      721 ctgaagaagc tgtcttggga taacctgtta ctcgtcccga cggtgttggt tcgaactcta
      781 ttattctgta gtctagtgaa gatacttggc ctgattcaat ggggcggcgt gagtcggaca
      841 ccacgacctc ggttgatgag gccgatcgaa tcaataatca cccctgtgcg gcttcgagga
      901 cggctggctc cgctgcaggc gctcgcctat gacgtcttgt tctgggtgat caggcgtagc
      961 gtgatcggaa ataccatgac ataaaacaag aggagcccac cgacgcaata gcctgtcatc
     1021 ccagcgcttt ccttcagatc gctgatcggc tagctatggg acttgcacca ccgtgacagt
     1081 ccagtgcgca ttgcttgaga ttccaagaga gtacgtcccc cctgtcgctc gatacgtcgc
     1141 cccgccagca tcgccgatcg aatgccccag tgccttgagg gattgtcctt cagcatcgag
     1201 aagagagagc gagaagtcat tcgtggcggt ccaccgaacc tcccacccat cttccacggt
     1261 gaaggcccga gtcgtcactg atccatttcc ggtgagttcc tgcacaatct gttccccccc
     1321 agcgaatgcc acccccgcaa gcagcaacca gctacatatt actgcactga tcagtcgata
     1381 catgacattc ctcctaccaa aattgtctat ttcgtcatag ccgtatcggc agctcttcga
     1441 taacctacgg gccggagatc gtacaaagaa ataggtttta tagcctaggt acaatgcttc
     1501 tcctgggctt gatctttttg gtgtgcgagt gtaaggaccg tgtagcactt cggcatcaac
     1561 ggcttgagcc aaggtcaagc aggctatttg gatgagcgtt caggagaatc gctgatggcc
     1621 gctccctccg gtcgcatgag tcacttgatt gcggtcctct gcagccgacg agcccaggtc
     1681 ccacgtgctt ggagaatgga gagtgaaagt cactaggagg ttacacggct cccatcctca
     1741 ccggagtaag ctccccggcc ttccaaaagg aaatgcctgc gaaaagggaa cccgacgaaa
     1801 aaggaaacct gacacccagt gagaaatatg gccccctgac tgtttcacgc ctctatgctc
     1861 aagacggacc acttcctgtc tcttacttag tggtccgaca gcccggatgg ttgcagccgc
     1921 aaccatctcc cgtacgggcg gagcggcact cgtcagaaca gaattctttt cctgcttcag
     1981 cgacacatgt acaccgagga tgggcgcatt tcttctcagc catgattcct cctagaggta
     2041 cattactgtg agcacacgtt cgtaccgtgc acacgtgcgt tctcctcctt cagtcatgta
     2101 ctgccgcggg ggaggcaccc ccctcccttt cggctgcaac gcccaagaaa cgtaccgggc
     2161 gctcgccggt cagatgctcc ggagtaatga cataggtccc gaacgtactg gggatccgga
     2221 tatttttcgc cgttggctca ctgaatcccg aaaacacata ggcgagacca ccgacgatgc
     2281 cacctgcgat cgcaaatgcc gctttaaatg ggaaatacag gattgtggcg gcagctgagg
     2341 ccgcttccat accggcccca taagccgtcc cttgctgtat atcagcggac gatgtgtcag
     2401 cggacgagga atgactccaa gccagcggga caagcatcga gcagtaggtg gagacaatga
     2461 tgaaaaccac gatggggagt ctcacctgaa tcgcttcatt tctttcattc atcaaaccaa
     2521 cagcgaccac catcgagatg attcgagttt tcatcggtgt ccttcgtatt gcgtatagtg
     2581 ttgaattact gtcgcgtagg accaccgaat ctgtaactgg gaaaacacct tggaagagag
     2641 gtcgagagag acgagacagc aatgagagat cgaatagagg ccttttaagt cccgacgcca
     2701 tccacactca tgctctcgtt gaccgtctga tgctttagtt gtgatctccg ctaccacgac
     2761 cttacccccc gtgtcttttc ctagtatgga actcccctag acttcgttag gattggatga
     2821 acaacttcta agggacgaag gatagtcaac ccataatcag actcaagcat cagcaccata
     2881 ttatttaaac catttagacg ctaagggagg acgcggatat tatgagccac agacacgatc
     2941 atgatgatga gggactaggg gatgcgaggg gatggtctgc ctttatggcc ggagcgttga
     3001 ttggggcgg
//
LOCUS       DATNDD010000278         2114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2803107, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000278 DATNDD010000000
VERSION     DATNDD010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803107"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VN647_06645"
     CDS             complement(<1..277)
                     /locus_tag="VN647_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWG96745.1"
                     /translation="MLPFSDLSIALAIFAVSYGFIITERIHKTIVALGGATLMIVSGV
                     MTQDEAFYSHEFGVDYNVVFLLIGMMIIVNIVRDTGLFEVLAIWSAKR"
     gene            432..>2114
                     /gene="glgB"
                     /locus_tag="VN647_06650"
     CDS             432..>2114
                     /gene="glgB"
                     /locus_tag="VN647_06650"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWG96746.1"
                     /translation="MSTLSRQHIEQLVTGSHWDPLAILGPHTREQNGSASLVIRCFLP
                     EADQAAVLVPTPEQRAFPMSRIHESGLFEAVLPQAPHSARYRLRVTDYAGRVSERYDP
                     YAFPPLLSDFDLHLFSEGTFFKAYETLGSHLRTIDGITGIHFVVWAPNAGRVSVVGDF
                     NQWDGRRHPMTNRGATGLWELFIPGLTDGTLYKYEIRPRGQDAILLKADPYAMASELR
                     PKTASVARDIAYQWHDQTWMESRRKRDPLQSPVAVYEVHLGSWMRVPEDHNRWLTYRE
                     LAQKLIPYVKDMGYSHLELMPVTEHPFDGSWGYQSTGYFAVTSRYGTPEDFMAFVDAA
                     HQAGIGVLMDWAPAHFPDDSHGLSQFDGTHLYDHADPRLGYHPEWHSRIFNYGRVEVR
                     TFLMNSALFWLDRYHIDGLRVDAVASMLYLDYGRNAGEWIPNQFGGHENLDAVLCLKD
                     LNVLVHRDFPGAMMLAEESTSWAGVSRPTYTGGLGFTFKWNMGWMHDMLQYFSHNPIH
                     RMYHQNQLTFGLLYAFNENFMLPLSHDEVVHGKGSLINKMPGDEWQQFANLRS"
BASE COUNT          451 a          606 c          577 g          480 t
ORIGIN      
        1 cacgttttgc cgaccaaatc gccaacacct cgaagaggcc ggtgtcacgg acaatgttca
       61 cgatgatcat catcccgatc aacagaaaaa cgacgttgta atccacgccg aactcgtgcg
      121 agtagaacgc ttcgtcctgc gtcatcacgc ccgagacgat catgagtgtt gcgccaccca
      181 aggccacgat ggtcttgtga atccgctccg tgatgatgaa gccatacgaa acggcaaaga
      241 tcgctaaggc gattgagaga tcggagaacg gcaacatgcg tgtcgattag gtggttatga
      301 gtcgatcggg tagatcatag aggcgctctg gacacgtggt gcgtaccaga gaggtggcaa
      361 ttctcgtcgt gcttctttca taatggatga acccgctggt tcgatacaca gaaacgttgg
      421 agcttaccat catgtctacg ctttcgcgac agcatattga acaactcgtc accggttcgc
      481 actgggatcc cttggcgatc cttggtcccc atacacgcga acagaatggt tctgcttcgc
      541 tcgtgatccg ctgtttcctg ccggaagccg accaagcggc cgttctggtc cccacgcccg
      601 agcaacgggc gtttcccatg tcgcgcatcc acgaatccgg cctgtttgaa gcggttctgc
      661 cgcaggcgcc gcactcagca cgataccggc ttcgggtcac cgattatgcc ggccgggttt
      721 ctgagcgcta tgatccctat gcatttccgc ccctcctttc cgattttgat ctgcatcttt
      781 tttctgaggg gacctttttc aaagcctacg aaactctcgg ttctcatcta cggacaatcg
      841 acggcatcac gggcatccat ttcgtcgtct gggctcccaa cgcggggcgc gtgagcgtgg
      901 tcggtgattt caatcaatgg gacggacggc ggcatcccat gacgaaccgt ggcgcgaccg
      961 gcctgtggga actctttatt cccggtctca ccgacggcac gctgtacaaa tacgagattc
     1021 gccctcgcgg gcaggatgcc attcttctga aagcggatcc gtatgccatg gcctcggaac
     1081 tcaggccgaa aaccgcatcc gtcgcacgag acatcgccta tcaatggcac gatcagacct
     1141 ggatggagag tcggcgaaag cgggatccgc tccagtctcc cgtggcggtc tacgaagtgc
     1201 acctcggctc ctggatgcgc gtgcctgaag accataaccg ttggttgacc tatcgagagt
     1261 tggcccaaaa actcatcccg tacgtcaagg acatggggta cagccatctc gaattgatgc
     1321 cggtgacgga gcatcccttt gacggttcgt ggggctacca atccaccgga tactttgcgg
     1381 tgaccagccg ctatggcacg cccgaggact tcatggcgtt tgtagatgcc gcccaccaag
     1441 ccggcattgg cgtgctgatg gattgggctc cggcacattt ccctgacgat tcgcacggtc
     1501 tctcgcaatt cgacggaacg catctctacg accacgccga tccgcgcttg ggttaccatc
     1561 ccgaatggca cagccgtatt tttaattatg gccgggtcga ggtacggacc tttctgatga
     1621 acagcgcgtt attttggttg gaccgctatc acatcgatgg tctccgcgtc gatgccgtgg
     1681 catcgatgtt gtacctcgat tacgggcgaa acgcaggtga atggattcca aatcagttcg
     1741 gaggccatga aaacttggat gctgtgttgt gcctgaagga tctgaatgtg ttggtgcacc
     1801 gggactttcc gggagccatg atgctggcgg aagagtccac gtcctgggcc ggcgtgtcac
     1861 ggccgaccta tacaggcggg ttgggattca cctttaaatg gaacatggga tggatgcacg
     1921 acatgctcca atacttcagc cataacccga tccatcggat gtatcatcag aatcagctta
     1981 ccttcgggct cctctacgcc ttcaatgaaa atttcatgct tcccctctca cacgacgaag
     2041 ttgtgcatgg caagggttcg ctcatcaaca agatgccggg agacgaatgg cagcagttcg
     2101 ccaacctccg ttca
//
LOCUS       DATNDD010000279         9319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4240828, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000279 DATNDD010000000
VERSION     DATNDD010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4240828"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VN647_06655"
     CDS             complement(<1..441)
                     /locus_tag="VN647_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96747.1"
                     /translation="MTRQQIFSIVFFSLLVLLLYQIGLMFKPFLFSALWAGLLAHWAF
                     PLHVRLTRLFRGRDALSAGMLTVGALGIVIVPLVAMGMMLVREAGAAEQEIRLWISAG
                     GLQRLPDQVAAVPFIGGWLKSAVSGTTTPTLSLEQSLMAGVKELS"
     gene            complement(581..1024)
                     /locus_tag="VN647_06660"
     CDS             complement(581..1024)
                     /locus_tag="VN647_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWG96748.1"
                     /translation="MKKTGYADDGNITLLAKGVELKGEIKVDGTVRIDGRLEGDVHTK
                     GEVIVGEDGVVKGAIHADSLISSGRIKATVTATGKIQLLKTAVLIGEVHCPTMLMEEG
                     AKFQGVSDMGVTAWPEEAPRLPGNVRDMNAHRGKAVVLIGRDPDL"
     gene            complement(1131..3443)
                     /locus_tag="VN647_06665"
     CDS             complement(1131..3443)
                     /locus_tag="VN647_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWG96749.1"
                     /translation="MSAQTSPRLVSLARRLLIPFLIVCGVTITLAGGYGVFLSTSLAL
                     PKSDEHPPLLIYGAPFFLTPGLHPVDSGLLDHLQRLEYKPVSGTPRVAGEYFAAKDSI
                     EIFLHAQEENRLPARLVRLILVNGIVTEVLSVSDRQPLSLVSLEPVLISGMRSGSRQV
                     REWIPLDRVPPTLIKTLLLIEDRRFFSHFGVDPIAIGRALWVNVTRGVVVQGGSTLTQ
                     QLAKNLFYSPKRTLGRKLRELMAAMTLEFKYRKEEILESYLNEIYLGQAGPVSIYGVG
                     EAAHRYFSKNLDELSIDETALIVGLIKGPNAYSPVKSVEDATKRRNVVLRRLGEEGIL
                     TEDSVARAMNRPVKVMLNQDVLTDAPYFVDHLLREIEQGIGMDIPDGARIYSTLDPRV
                     QRIVAQVLQEGLTKLEKSYPALVGVEPPLQGAAVVLDVKTGHVLAMVGGRNYRLSQFN
                     RAVQAHRSAGSLFKPFVYLAGFEAARDQGAAGLTPTTLLVDEPVTLESDTGSWSPQNY
                     DRQYRGQVTVRTALEQSLNVPAVRTAHRTGITALTNLLHAFGITTPLADNLSLALGSS
                     SVSLLQITSAYAGLANGGVVIHPVALSNMVREGRETIWSPPLDRRQAATPQGAFLITS
                     LLKGVMDRGTGAKARALGVRGPVAGKTGTTDGYRDAWFIGYTPNIAIGVWVGFDDERP
                     VKLTGAQAALPIWSELAVRLIPRDPQDFEMPVGVVRRRVDPKSGQLATSQCPEKTFEF
                     FITGTEPTIYCEVHGGGFWERLKQTFGLPQ"
     gene            complement(3613..6702)
                     /locus_tag="VN647_06670"
     CDS             complement(3613..6702)
                     /locus_tag="VN647_06670"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HWG96750.1"
                     /translation="MVIEQGGNALVADVDSTNLDTGTLTVSIPAGGDSAEDVLSIRHQ
                     GTGAGQIGVSGSTVTYGGTTIGTFAGGSSGSNLVITLNGNATPTAVTVLVKNITYQNT
                     DTNAPTTGARTVRFVLTDGDGGTSANYDTTVTVSAVNDAPVLTDTALTLTVAEDAGVP
                     SGAVGSVVSAFTGGITDVDSGASKGIAITGSNQTNGTWYYTTNGGSTWTAVGTVSNTS
                     ALLLADNGSTRLYFAPKANYNGTSSAALTVRAWDQSSGTAGTKVSTASNGGTTAFSSA
                     TDTVAVTVTAVNDAPTDLSLSANTVAENAANGTVVGTVSGTDPDAGDTQTYSFTDSAG
                     GRFAINSSTGQITVADGSLLDYESAASHSVTVRVTDSGGLTYDETFTITLTNVNEVPT
                     SADATVTINEDTSHTLTTANFDFSDVDAGDTMSAVRIDSLPSAGTLTLSGVAVTAGQV
                     VTMADITAGNLVFTPAADANGTGYANFTFSVRDSNNAYDTAPHTLTFNVTAVNDAPVN
                     QVPGVQTVNEDTPLSLSGLSVNDVDGNLSTVQLAVGNGTLNVTLSGAATITSGSNGTN
                     ILTLSGSQADLNATLASLVYQGSLNYTGADTLTVTSTDSNAVTDVDMVAITVTAVNDA
                     PTDLSLSANTVAENAANGTVVGTVMGTDPDAGDTKTYSLIDSAGGRFAINSSTGQITV
                     ADGSLLNYENATSHGVTVRVTDSGGLTYDETFAINLTNVNEAPTSITLSGDTVVENAA
                     NGIVVGTVNAVDPDSGDTKTYTLTNSAGGRFAINSSTGVLTVANGALLNYEAATSHMI
                     TVRVMDAGGLAYDKSFIVTLTDAYEPLPPLNMSSSSATSHSIVTRENGTALSSLPDVS
                     SETGKVEFRPPDDRRKAIEIMTETSPPTATTNLEGKHLSVVREEFFGKVESTTEEKKF
                     LAAPLSENKTEGKVTAQDHGNSALLISDHHVDPQQLDRTSESAISTTMAVGLAGVVLQ
                     GAVGTKEKLIANIRRPREHAEETSLDMESELSSEDDSHSPHNESGHGDSVPNKPGST"
     gene            complement(6585..7451)
                     /locus_tag="VN647_06675"
     CDS             complement(6585..7451)
                     /locus_tag="VN647_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HWG96751.1"
                     /translation="MFDAAAAATAAEVQSAQVAQDQAEAAVSSEAAGDDQTTEQNESQ
                     DLLQALATYNPGESTIEVVFVDPTVPDCQTLLAGIGPNAEVIMLDPTRDGVEQIAESL
                     AGRSGIDAIHLISHGSSGELQLGAGTLTAGSMSGEYADELATIREALSDQADILVYGC
                     DFAEGQNGQEAATLLSQLTGADVAASTDATGFAGLGGDWVLETQTGTIETQIAVDDGV
                     QANWVACSPVKRPPRSPISAATAWRTARATGRWSSSRAATRWSPMWIAPTWIPAPSPC
                     RFRPEATAPRMC"
     gene            complement(7601..7852)
                     /locus_tag="VN647_06680"
     CDS             complement(7601..7852)
                     /locus_tag="VN647_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96752.1"
                     /translation="MFKAVQRRYYRRPFKLRQSDRAEILVSDKAAQSVYSQQCLIASQ
                     KQFISVTICSYWMEARSLVWSLPSFSGISSIERIEPMKT"
     gene            complement(7943..9196)
                     /locus_tag="VN647_06685"
     CDS             complement(7943..9196)
                     /locus_tag="VN647_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWG96753.1"
                     /translation="MSLAVNLNPFMRFDGYYLLADSLSIPNLQDRAFAFGQWQLRRLL
                     FGCFSSPPETTSVALRRTLIAYAWAIWLYRLVLFTGIAVMVYQYFFKALGIILFLVEI
                     VWFIAMPIAREITSWWTNRAFYAASSRAWGTAVILGILLSLVFIPIPTSVSAPAVLQA
                     ASYATIYASMPGRISQVLVRNGQRVMAGEQIAVLENPSLEKEVRLAEIKAAMWTYRLG
                     RQAGHDQDRAQRQVIAESLKAELAELAGLESKQANLILKAPMAGMIRDHADSLIPGRW
                     IDQKLPVFYLIDAGHAVIEGLIPVDELAYIDVGQSARFIPADLTSPSVEAQVAKIAEV
                     DEQDVAISYFASIFGGDMAVRKDDKGRLNPEVSVYRVRLNLRDAIQDVDQAVLGHVQI
                     DGQPSSLAKRAWDHTVAVLIRESGF"
BASE COUNT         1934 a         2830 c         2546 g         2009 t
ORIGIN      
        1 actgagttcc ttcaccccgg ccatgagcga ctgctccaag gaaagcgtag gcgtcgtggt
       61 accggagacc gccgatttca accaaccgcc gatgaaggga accgccgcca cttgatccgg
      121 cagacgttga agtccgcccg ccgagatcca taaccggatt tcttgctcag ctgcaccggc
      181 ctcccgtacc aacatcattc ccatcgcaac caagggaacg ataacaatcc ccaacgcccc
      241 gacggtcagc atgccggcag agagtgcgtc cctaccgcgg aacagtctcg tcaaacggac
      301 atggagagga aatgcccaat gggccaacaa acccgcccac aaggccgaga acaagaacgg
      361 cttgaacatc agtccaattt gatagaggag caataccagg agggaaaaga acacgatgga
      421 aaaaatctgt tgtcgagtca tgggagatgt cggagtctct tgaagaggta tcgagtaaat
      481 agcagatccg gcagcgtctg tcacggtagg aaagaacggc atggaagcag cgagacctcc
      541 cgatgtaaga acagcaccga gagcaacggg gggaatgagc ttacagatcg gggtcccttc
      601 cgatcagcac aaccgctttc ccacgatgcg cattcatgtc acgaacatta ccgggcaacc
      661 gaggcgcttc ttcaggccag gccgtcacgc ccatatcgct cacgccctga aattttgctc
      721 cctcctccat caacatcgta ggacaatgca cttcgccgat caaaaccgcg gttttgagga
      781 gttggatttt tccggttgcc gtaaccgtgg ctttgatacg tccgctgctg atcagtgagt
      841 cggcatggat ggctcccttc acaactccgt cttcccccac aatgacttcc cccttcgtgt
      901 gcacatcgcc ttcgagacgc ccatcgattc tcacggtgcc gtcgactttg atttcacctt
      961 tcagttcgac accttttgcc aacagcgtaa tattgccatc gtctgcatac ccggtctttt
     1021 tcatgggggc tcctcggtgc aggccaacct tccaccaggt taccttagga ccggggatac
     1081 acctaatagt ttgtggaatc cggcactcct atgctcgcct ccaacttgtt ttattgcggt
     1141 aaaccgaacg tctgcttcag ccgttcccag aatccgcctc cgtgaacttc gcaatagatc
     1201 gtcggttcag tccccgtgat gaagaactca aacgttttct ccgggcattg cgaagtcgca
     1261 agttgcccgc ttttgggatc gaccctccgc cggacgaccc caaccggcat ctcaaaatcc
     1321 tgaggatccc gcgggataag ccgaaccgcc aattcactcc atattggaag ggctgcctgc
     1381 gcgccggtca acttgacggg ccgttcatca tcaaaaccca cccaaacgcc gatcgcaatg
     1441 ttaggggtat atcctatgaa ccaggcgtct cgatacccat cggtcgttcc agtcttgcct
     1501 gccacaggac ctcgcacccc taacgctctt gccttagccc ccgtgccccg atccatcact
     1561 cctttcaaca acgacgtaat gagaaatgcc ccttgcgggg tggctgcctg acgtcgatcg
     1621 agaggaggac tccaaatcgt ttcccttccc tcacgcacca tgttggacag cgcgacagga
     1681 tggatgacaa cccctccatt ggcaagcccg gcgtaggcag acgtaatctg aagcagtgaa
     1741 actgaagaac tacccagcgc caaggacaaa ttgtcggcca acggtgtggt aattccgaac
     1801 gcgtgcagca gattcgtgag cgcagtaatt ccggttcggt gtgcggttcg aacggcggga
     1861 acgttcaagg actgctcgag cgccgttcgg accgtcacct gtcctctata ctgccgatcg
     1921 taattctgag gcgaccagga ccctgtgtcg gattccagtg tcaccggctc atccacgagt
     1981 aatgttgtcg gagtcagccc ggccgcacct tggtcacgtg cggcctcaaa tcccgcaaga
     2041 tacacaaaag gcttgaagag agaccccgcc gatcgatgag cttgcacagc ccggttgaat
     2101 tggcttaggc gatagttacg accgccgacc atcgccagca cgtggccggt cttcacatcc
     2161 agtacgaccg cggctccctg aagtggaggc tcgactccga ccagagccgg ataactcttt
     2221 tcaagtttcg tcaacccttc ctgtagcacc tgagcgacga ttcgctgaac tctgggatca
     2281 agggttgaat aaattcgcgc gccgtccggt atgtccattc ctatcccctg ctcgatttct
     2341 ctgagcaagt gatcgacaaa gtacggcgca tcggtgagga catcttgatt cagcatgacc
     2401 ttcaccggcc ggttcatggc ccgagccacg gagtcttctg tcaagatccc ctcttccccc
     2461 aaacggcgaa gcaccacatt tcgacgcttc gttgcatcct caacgctttt tacaggagaa
     2521 taggcattgg gccccttgat caatccgacg atcaacgccg tttcatcgat cgacaactcg
     2581 tcaagattct tactgaagta gcgatgggcg gcttctccta caccatagat cgaaaccggt
     2641 ccggcttgac cgagatagat ctcgttcaaa taactctcta gaatctcctc tttccggtac
     2701 ttgaactcaa gcgtcatcgc ggccatcaat tcccggagtt tccgcccaag ggttcgcttg
     2761 ggagaataga agaggttctt ggccaattgc tgggtgagcg tgctgcctcc ctgcacgacg
     2821 acgccgcgag tcacgttgac ccacagcgct cggccgatcg cgatggggtc aaccccgaaa
     2881 tgggaaaaaa accgacgatc ctcgatgagc agcaacgtct taatcagggt gggagggaca
     2941 cggtccaggg gaatccattc ccggacctgc ctggagccgg agcgcatccc gctgatcaaa
     3001 accggctcga gtgataccaa agaaagcggt tgcctatcgg acacggacag cacctcagtg
     3061 acgatgccgt tcaccaatat caaccggact aatcgcgcag gaagtcggtt ctcctcctgg
     3121 gcatgcagaa aaatctcgat tgagtctttt gcggcgaaat attcaccggc tacacgcggt
     3181 gtgcccgaga ccggcttgta ctccaaccgt tgcagatggt cgagcaatcc cgaatccaca
     3241 ggatggagtc ccggcgtcag aaaaaacggg gcgccataga tcaacagggg tggatgctca
     3301 tcgcttttcg gtaatgccaa actggttgag agaaagacgc cataccctcc cgctagagtg
     3361 atggtcactc cgcatacgat aaggaatgga ataaggagac gccttgccaa cgagacgagc
     3421 cgaggtgacg tttgagccga catggcctca gcttaccctg tatcttcaga caaggaaact
     3481 agtccaagat gggaaacgcg ccccgggagg tctgtttagg ctgtcgtagc gaggttgcct
     3541 gatcaattcg gcagattaaa aagcaccgaa cataggagtt ttatataagc gctccgaagt
     3601 acggttgtgg cttcaagtag agcctggttt gtttgggacg gagtctccat gccccgattc
     3661 attgtgcggc gaatggctgt cgtcctcgct cgagagttca gactccatat ccagcgaagt
     3721 ttcttcagca tgctcacggg gccgccgaat gttggcgatc agcttttctt tggtccccac
     3781 agctccctgt aggacaaccc ctgccagccc aaccgccatt gtcgtgctga tggcactctc
     3841 gcttgtacga tctaattgtt ggggatctac gtgatgatcc gatataagta aagcggagtt
     3901 tccatgatct tgcgctgtga ccttcccttc ggtcttgttc tcactgaggg gagcagctaa
     3961 gaatttcttt tcttctgtcg tcgactctac tttcccaaag aactcttccc gaacgacact
     4021 caaatgcttt ccctcaaggt tcgttgtagc ggttggtggg gaagtttctg tcattatctc
     4081 gatcgccttc ctacggtcgt ccggtggacg gaattccacc ttgccggtct cactcgatac
     4141 atccggcaag gaagacaagg ccgttccatt ctcacgcgtg acaatcgaat gacttgtcgc
     4201 agaagagctg gacatattta gaggcggcag cggctcatac gcatcggtga gagtaacgat
     4261 aaaagacttg tcgtaggcca accctccagc atccatgacc ctgacagtga tcatatggct
     4321 ggttgccgct tcgtagttca agagcgcgcc attggcgacc gtgagcaccc cggtgctgct
     4381 gttgatggca aaccgcccgc ctgcactatt ggtcaaggta taggttttcg tatccccgct
     4441 gtcgggatcg accgcattga ccgtgcccac cacgatccca ttcgccgcat tctccaccac
     4501 ggtgtcgcca ctcagggtaa tactggtcgg tgcttcattc acgttagtca ggttaatggc
     4561 gaaggtctcg tcataggtca gtccgcccga gtccgtcacc cgcaccgtca caccatggct
     4621 ggtcgcattc tcgtagttga gcaagctgcc atcggccacc gtaatttggc ctgtgctgct
     4681 attgattgcg aatcgcccac ccgcactatc gatcaagctg tacgtcttgg tatcgccggc
     4741 atctgggtcc gtccccatca ccgtgcccac cacggtcccg ttcgccgcgt tctccgccac
     4801 ggtgttcgcc gacagactca gatccgtcgg cgcatcgttg acggccgtca ccgtgatggc
     4861 caccatatcc acatcggtca cggcattgct gtccgtgctg gtgacggtca gcgtatcggc
     4921 gccggtgtag ttcaagctgc cttggtaaac caggctcgcc agggtcgcgt tgaggtccgc
     4981 ctggctcccg gagagggtca ggatattcgt gccgttgctg ccactggtga tcgtggcggc
     5041 tcccgagagg gtgacgttta acgtcccgtt gcccaccgcc aactgcacgg tgctcaggtt
     5101 gccgtccacg tcattgacgc tgaggccgct gagactgagc ggggtgtctt cattgaccgt
     5161 ctgcaccccc ggcacctggt tcaccggcgc atcgttcacc gcggtgacat taaaggtgag
     5221 tgtatgcgga gccgtatcgt acgcgttgtt gctgtcacgc accgagaagg tgaagttagc
     5281 atagccggtg ccattggcat cggccgctgg cgtgaacacc agattgcccg cggtgatgtc
     5341 cgccatggtg acgacctggc ctgccgtcac ggccacgcct gacagggtta aagtgcccgc
     5401 gctaggtaag ctgtcgatcc gcaccgcact catcgtatct cccgcatcga cgtcactgaa
     5461 gtcaaagttg gcggtggtga gggtgtggga tgtatcttcg ttgatcgtca ccgtcgcatc
     5521 ggcgctggtc ggcacttcgt tcacgttggt cagagtgatc gtaaacgtct cgtcgtaggt
     5581 cagcccgccg ctatccgtca cgcgcacggt cacgctatgg ctggcggcac tctcgtaatc
     5641 cagcaagctg ccatcggcca ccgtgatctg gccggtgctg ctgttgatcg cgaaccggcc
     5701 ccccgcacta tcggtgaagc tgtacgtctg cgtgtccccc gcatccggat cggtgccgct
     5761 caccgtcccc accaccgtcc cgttcgctgc gttctccgcc acggtattgg ccgaaagact
     5821 cagatctgtc ggggcatcgt tgacggccgt cacggtcaca gccacggtgt cggtggcact
     5881 ggagaaggcg gtcgtcccgc cattgctcgc cgtactcacc ttcgtccccg ccgtgccgct
     5941 gctctgatcc cacgcccgca ccgtcagagc cgcgctgctc gtcccgttgt aattggcctt
     6001 cggcgcaaag tacagccgcg tgctcccgtt gtccgccaac aacagggccg aggtgttact
     6061 caccgtcccc accgccgtcc aggtgctgcc cccgttcgtc gtgtagtacc aggtcccatt
     6121 cgtctggttg ctccccgtaa tcgcgatgcc cttggacgcg ccactatcca catcggtgat
     6181 gcccccggtg aaggcgctca ccacactgcc caccgccccg ctcggcaccc ccgcatcttc
     6241 cgccaccgtg agcgtcaacg ccgtatccgt caacaccggc gcatcgttga cggcactgac
     6301 cgtcaccgtc gtgtcatagt tcgcactggt gccgccgtcc ccatcggtca gcacaaaccg
     6361 cacggtgcgg gcccccgtgg tcggcgcatt cgtgtcggtg ttctgatagg tgatgttctt
     6421 gaccagcacg gtgaccgctg tgggggtggc attgccgttc aatgtgatga ccaagttgct
     6481 gccgctgctg ccgcctgcaa acgtcccgat ggtcgtcccg ccatacgtca cggtgctgcc
     6541 gctcacgccg atctggcccg caccggtgcc ttggtgccgg atgctcaaca catcctcggc
     6601 gctgtcgcct ccggccggaa tcgacacggt gagggtgccg gtatccaggt tggtgctatc
     6661 cacatcggcg accagcgcgt tgccgccctg ctcgatgacc accgccccgt tgccctcgct
     6721 gtacgccagg cggtcgccgc tgagattggt gatcgtgggg ggcgtttcac cggcgagcag
     6781 gccacccaat tcgcctggac tccgtcgtcc acggctattt gcgtctcgat agtcccggtc
     6841 tgcgtctcca gcacccagtc gccgcccaat ccagcgaagc cggtggcgtc cgtgctcgcg
     6901 gcgacgtcgg cgccagtcaa ctgactcagc aacgtggccg cctcttgccc attctggcct
     6961 tcggcgaaat cgcatccata aacaagaatg tcggcctgat ccgacaaggc ttcccggatc
     7021 gtggccagtt cgtcggcata ctccccggac atggacccgg cggtgagcgt gccggcaccc
     7081 agctgcaact cccccgagct accatgcgag atgaggtgga tggcatctat gccgctgcgt
     7141 ccggccagcg actcagcaat ctgctcaacc ccatctcgtg tgggatcaag catgatcact
     7201 tctgcattgg ggccgatccc ggctagcagc gtttggcaat ccggcacagt ggggtcaacg
     7261 aaaaccacct cgattgtgga ctcgcccgga ttataggtcg caagggcttg aagaaggtcc
     7321 tgtgactcgt tctgctcagt ggtctgatca tcgccggcag cttcggatga tactgcggct
     7381 tcagcctgat cctgagccac ctgcgcagac tgcacttcgg cagctgtcgc tgcggccgct
     7441 gcatcgaaca tcaagcgagg ctcgagcgag agaagggatt gttttgtctt cggagcctgg
     7501 ggcgcgcctt ccgagtctgc tcggtcttgg tgtcccgtga agtcgatttt gggttcttgc
     7561 gaaagaacat atcagtgtat tttgtaatcg agacagtgtg tcaggtcttc atcggctcga
     7621 tgcgttcgat gctggagatc ccactgaagc tgggcagact ccagaccagc gatcgagcct
     7681 ccatccagta agaacagatg gtaacggaga tgaactgctt ctgggatgca atgaggcatt
     7741 gctgtgagta cactgattgc gctgctttgt ccgaaactag gatctcggct cggtcggatt
     7801 gccgcaactt gaatggacgg cggtaatatc gacgctggac cgctttgaac atgagggagg
     7861 atagttcttc ccgttctgcc tatcggacca attccaccgg accatagtcc cgaaggccgc
     7921 ctagctggat ccagccggtt gcctagaacc cgctctctct gatcaaaacc gcaacggtgt
     7981 gatcccaggc gcgtttcgct aggctggagg gctgtccatc gatctgaacg tggccgagga
     8041 cggcttgatc gacatcctgg atggcatcgc gaaggttcag tcgaactcga tatactgaaa
     8101 cctccggatt aagtcggccc ttatcgtcct tccgcaccgc catatcgccc ccaaaaattg
     8161 atgcgaagta ggatatggct acatcttgct cgtcgacttc ggctatcttc gccacctgag
     8221 cttcaacgga tggactggtc aggtctgcag ggatgaatcg cgccgattgc ccaacatcga
     8281 tataggcgag ctcatcgacg gggatgagac cttcgatcac agcatgaccg gcatcgatga
     8341 gatagaaaac gggaagcttc tgatcgatcc atcggccagg gatgagcgaa tctgcatgat
     8401 ctcgtatcat cccggccatg ggggccttga gaatcaagtt cgcctgcttg gattcgagtc
     8461 cggccaattc tgccaattcg gccttcaacg actcagcgat cacttgccgt tgcgcacggt
     8521 cctgatcatg accagcctgg cggccaaggc gataggtcca catcgccgct ttgatttccg
     8581 ccaaccgcac ttctttctcc agcgatggat tctccaatac cgcgatctgt tcgccggcca
     8641 tgaccctttg cccgttacgg accaatactt ggctgatacg tccaggcatc gacgcataga
     8701 tcgtggcgta agaggcggcc tgcagaaccg cgggagccga gacgctcgta ggaatcggaa
     8761 taaacacaag cgagaggagg atgccaagga tgacggccgt gccccaggct ctcgatgaag
     8821 ccgcatagaa ggcacggttg gtccaccagc tggtgatctc ccgagcgatg ggcatggcga
     8881 tgaaccagac gatctcgacc agaaacagaa tgatgcccag tgctttgaag aaatactgat
     8941 agaccattac agcgatgccg gtgaaaagaa ccaggcgata gagccagatt gcccacgcat
     9001 aggcgatgag ggtccggcgc agagcgactg aggtcgtctc ggggggcgag gaaaagcatc
     9061 cgaacagcag cctgcgcagt tgccattgtc cgaacgcgaa ggcccgatcc tgaagatttg
     9121 gaatgctcag gctatctgcg agcagataat agccgtcgaa tcgcatgaac gggttcaaat
     9181 tcactgcgag gctcataatc acactagaac ctatctcaaa atagttgacg gcgtgaccag
     9241 cggatcccta taagtccggt catgcttcgt ctgcgcgatg accaatggga acggattcgg
     9301 gaatactttc ctgaagagc
//
LOCUS       DATNDD010000280         5280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2056485, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000280 DATNDD010000000
VERSION     DATNDD010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056485"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VN647_06690"
     CDS             <1..633
                     /locus_tag="VN647_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWG96754.1"
                     /translation="GIITVDDVVDVIREEATEDMLKMAGAIEEETGSKSSGFGSAKLR
                     LPWLFTNLVGSLLSGAILWYFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIRGLA
                     IGSVELTHVGPVFFREAKIGLVMGVACGVTLTLVAWVLHQGFLGMVVGASLIIAFLVS
                     TSMATIMPILLKRVGVDPAVAAGPFVTTANDITGISIYLTLATLFLEYLR"
     gene            684..1442
                     /gene="recO"
                     /locus_tag="VN647_06695"
     CDS             684..1442
                     /gene="recO"
                     /locus_tag="VN647_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWG96755.1"
                     /translation="MPLIKTTAIILRSRKWGEADRIVTLYSKDLGKIRGIARGARRQK
                     SRFGGAIEPFSVCCLNLFEKTGDSLFCISHVDLVRSSQVLREDLRLMASAARMVNVVA
                     AITPDGDPDPLLFDTLEQGLASLHESEDPTFMALLVQIRLLGLTGFRPQTDQCAACGK
                     RHFVGEPQFSPIAGGLVCLVCAARQRVRCVAFSRGSLLFLQQAIRLAPTVLTRLRAAG
                     QVRNEVEEAIEGYVTVVAGKRLPPVDFLSLALPG"
     gene            1468..1824
                     /locus_tag="VN647_06700"
     CDS             1468..1824
                     /locus_tag="VN647_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWG96756.1"
                     /translation="MAASALEPRDMEWLDKGVLGIQWSDGHKGIYPVRYLRQHCPCAA
                     CVDEWTGERRLKSEDVPILIMLQDIESVGRYAFQFKWSDGHDTGIYSYSLLRKLCQCD
                     ICQPVKPVESKSRRLM"
     gene            1839..2312
                     /locus_tag="VN647_06705"
     CDS             1839..2312
                     /locus_tag="VN647_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96757.1"
                     /translation="MTNISVAAIKTIWCLSGLLAFVLSVGCKSMPTLEQQEQLVQANN
                     LVLDQITTRAVVNAWGKPPLYHSEFSHFFVMPDFSVVPRSRVATGEAPRGWKAGVHAG
                     EGVYFAYPDRGWLLVFLDDRLVYKEELKAEEVHALAKSWAYEDRFKTRLDEVSRP"
     gene            2369..3844
                     /gene="glgA"
                     /locus_tag="VN647_06710"
     CDS             2369..3844
                     /gene="glgA"
                     /locus_tag="VN647_06710"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HWG96758.1"
                     /translation="MAASEAVPYAKTGGLGDVVGALAIELTKLGHHVTLLVPGYRGRA
                     TGESRQLAMRLSIPVDGKPVDVAVEEENVAVTGALHRLRVLFVRYDPYFDRQGLYQQD
                     HHDYPDNLDRFILFCRAVLEIVRDLIETNERKVDVLHLHDWQTALCAVYLKTLPHEYK
                     GLHRLKVLLTLHNMGYQGTFPGEQFVMMGLPPSLFSPSGLEYYGSINCLKGGIIFSDA
                     VSTVSPTYAKEIMTAEYGCGLEGVLAGRADGVKGITNGIDVATWNPESDLYLSAKYTA
                     DDLSGKQACKRALQRELGLPNRDVPLLAVIGRLTFQKGFDLLIDVIPELMSLDTQIVV
                     LGTGDQHLEQQFIAIKARYFDRIGLCLGFDEGMAHRIEAGSDMLIMPSRYEPCGLSQQ
                     YSLRYGTVPIVRCTGGLADTVVPFRPSTVKSRCATGFHFIEASADSLLSVLLLSLHTY
                     KDRQIWRSMIHAGMKADLSWGRSANLYVNLYRELRREKRES"
     gene            complement(3841..4335)
                     /locus_tag="VN647_06715"
     CDS             complement(3841..4335)
                     /locus_tag="VN647_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96759.1"
                     /translation="MNCDAFREGLVVGLFGAALLTAGCTTGPTPQLTELKNSGFMSLW
                     NTYADCKSTSDLAQATSDLKHLRSTSQEANEGFILPLPTHFERLVANPTNRVAVDVEA
                     MAAACALHTGELALNQGLTDIARDLLVSVIKLHKEEGSYYVLKAKTLLAKLGPGFNVS
                     FNIR"
     gene            complement(4530..5138)
                     /locus_tag="VN647_06720"
     CDS             complement(4530..5138)
                     /locus_tag="VN647_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWG96760.1"
                     /translation="MTEAVKGIDQTDALADQRAGSGIVVLSASMQLLHMNRQATELAK
                     KINAVEHGRTTTISAHGVLPTALTELCAEIIKALHIRTEAKDWEQFELKRVTGDPNQP
                     ILLRGFGLPDRGGIQNARLVVTMEELGRKQNLNTEHARERFQLTNREQSVVEHLAKGW
                     TNKEIANALQITEQTVKEHIKHIMRKTNATTRTGILVQIFNS"
BASE COUNT         1203 a         1229 c         1464 g         1384 t
ORIGIN      
        1 ggcatcatca cggttgatga cgtagtcgat gtgatcaggg aggaagcgac cgaagacatg
       61 ttgaagatgg ccggggcaat tgaagaagaa acgggctcga agtcttcggg cttcggctcg
      121 gcaaaactgc ggttgccatg gctcttcacc aacctagtcg gcagtctcct gtccggtgcg
      181 atcctttggt atttccgtta tacgattcag gaagttgtgg cgatcgtgag tttcattcct
      241 gtgattgcgg cgatgggtgg taatgtgggg ctacaatcct cgacgttgat tattcgcggg
      301 ttggccatcg gttctgtgga actcacccat gtagggcctg tcttttttcg tgaagcgaag
      361 atcggcttgg ttatgggggt ggcctgcggt gtgacgctga cactggtcgc atgggtttta
      421 caccaagggt ttttgggcat ggtcgtcgga gcttcgctga tcattgcctt tctggtgtcg
      481 accagcatgg cgacgattat gccgattctt ctcaaacggg tgggggtcga tccggccgtt
      541 gcggctggtc ctttcgtcac gacggctaat gacatcacgg gtatttcaat ctacctgact
      601 ttggccactc ttttcttgga gtatctccga tgatacggac aggtacgtcg ccacgcttct
      661 gtcgcggtcg ggagtagaga gccgtgccgc tgataaagac gacggcgatc attttgcgga
      721 gccgcaagtg gggcgaagcc gatcgaatcg tcacgttgta ttccaaggat cttggtaaaa
      781 tccgaggcat agctcgtggt gcccgtcgtc agaaaagccg cttcggaggt gcaatcgaac
      841 cgttcagcgt gtgttgcctg aacctatttg aaaaaacagg ggattcactg ttttgtattt
      901 cgcatgttga tctcgtgcgt tcgtctcaag tgctgcgaga agaccttcgt ttgatggcat
      961 cggcggcgcg aatggtcaat gtcgtagcag cgatcactcc tgatggagat ccggacccgc
     1021 tcttgtttga taccctggag caaggcctcg cctcgctcca tgaaagtgaa gacccgacct
     1081 ttatggcgct ccttgttcag atccgactgc tgggattgac agggtttcgt ccgcagacgg
     1141 atcagtgcgc cgcctgtggg aagaggcatt tcgtcgggga gcctcaattt tctccgatcg
     1201 ccggaggcct cgtatgcctg gtctgtgccg cacgtcaacg agttcgatgt gtcgcattct
     1261 cacgaggcag ccttttgttc ctccaacaag ccattcggct ggctccgaca gtactcacac
     1321 gattgcgagc tgcggggcaa gtacggaatg aggtggaaga agcgattgaa ggatacgtaa
     1381 cggtcgttgc aggaaagcgg ctaccgccgg ttgacttcct atcgctcgca ttgccaggat
     1441 gaggcagaca cggtgagagg tgaaaacatg gcagccagcg ctttggagcc tcgagatatg
     1501 gagtggctcg acaagggagt gctgggcatc cagtggagcg atggccacaa gggtatctat
     1561 ccagttcgtt atctgcggca gcactgtccc tgcgcggctt gtgtcgatga gtggactggc
     1621 gagcggcgtc ttaagtcaga ggatgtgccg atcttgatta tgctccagga tatcgagtcg
     1681 gtgggacgct acgcgtttca gtttaaatgg agcgacggcc acgatacggg tatctattct
     1741 tattcactgc taagaaaatt gtgtcagtgc gacatctgtc agccggtgaa accagttgag
     1801 tcaaagtctc gacggctcat gtgaggctag gatacgacgt gaccaatatt tctgttgccg
     1861 cgataaagac gatctggtgt ttaagtggac ttttggcatt cgtattgagc gtaggttgca
     1921 agagcatgcc gacgctggag cagcaagaac agctggtaca agccaataat cttgtactgg
     1981 atcaaatcac caccagggca gtcgtcaacg cctgggggaa gccgccgctt tatcacagtg
     2041 agtttagcca tttttttgtg atgccggatt tcagtgttgt tcctcgatcg cgcgtggcga
     2101 caggagaggc tccgaggggg tggaaggccg gagtgcatgc gggcgaagga gtgtatttcg
     2161 catatcccga tcgtggttgg ctcctagtat ttctcgatga tcgtcttgtc tacaaggaag
     2221 agctcaaagc cgaggaggtg catgccctgg caaaatcctg ggcctatgag gatcgattca
     2281 aaacccggct tgacgaagtt tctcgaccct aagccctccg ctaatacccc gcatgacacc
     2341 agcatccgta gatggattga acatcgtgat ggcagcttcc gaagccgttc cgtatgccaa
     2401 gacgggtgga ttgggcgacg tggtcggcgc attggcgatt gagctgacga agttggggca
     2461 tcatgtgaca ttgctggtgc cgggttaccg aggtcgagca accggtgagt ctcgtcaact
     2521 agccatgcga ctttctattc cggtggatgg gaagccggtc gacgtagcgg tggaagaaga
     2581 gaatgtagcg gtgaccggtg cattgcatcg attgagggta ctgtttgtgc ggtatgatcc
     2641 atactttgat cgccaaggac tctatcaaca agatcatcat gattatccgg ataacctaga
     2701 ccggtttatc ttattctgcc gtgcggtcct cgaaatagtg cgagatttga ttgaaactaa
     2761 tgagaggaag gtcgatgtgc tgcatttgca cgattggcaa acagccctgt gcgcagtata
     2821 cctaaaaact cttcctcacg agtataaggg actccatcgg ctgaaagtgc tcttgactct
     2881 gcacaacatg ggatatcagg gaaccttccc cggcgagcag tttgtgatga tggggctgcc
     2941 cccatcacta ttttctccga gcggactcga gtactatggg tcgattaatt gtctgaaggg
     3001 cggcatcatc ttttcagatg ctgtttccac ggtcagtcca acttatgcga aggagatcat
     3061 gaccgcagaa tatgggtgtg gccttgaagg tgtgctggca ggtcgtgcgg atggcgtcaa
     3121 agggattaca aatggtatcg atgtcgccac ctggaatccc gagagcgacc tctacctatc
     3181 ggctaagtat acggctgatg atttgtcggg aaaacaagca tgcaaacgag cgctacaacg
     3241 tgagcttgga ctgccgaatc gtgatgttcc cttattggct gtgatcggtc ggttgacctt
     3301 ccagaaaggc tttgatctcc tgatagatgt gattcctgag ctcatgtcct tagatacaca
     3361 aatcgtcgtg cttggtactg gagatcagca tttggaacaa caatttattg caatcaaagc
     3421 cagatatttt gatcgcattg gcttatgcct tggctttgat gaagggatgg cgcatcgtat
     3481 tgaagctggt tcggacatgt taattatgcc gtctcgctat gagccatgtg gattaagtca
     3541 gcagtatagc cttcgatacg gcacggtacc tattgtgcgc tgtaccggag gattggcgga
     3601 tactgttgtt ccctttcgac cgtcaacggt caaatctaga tgtgcgactg gcttccattt
     3661 cattgaagct tcggctgact ctctactctc ggtgctcttg ctctcacttc atacgtacaa
     3721 agatcggcag atatggcgat ccatgatcca tgcgggaatg aaggccgatc tatcttgggg
     3781 gcgttcggca aacctatacg taaatttata tcgagaactg cggagagaaa aaagagagag
     3841 ttagcgaatg ttgaaagaaa cgttgaatcc tgggccgagt tttgccagca atgtctttgc
     3901 ctttagaacg taataagacc cttcttcttt atggagcttg ataactgaaa ccaggagatc
     3961 acgagcgata tcagtcagtc cttgattaag ggctagttct cccgtgtgca aggcacaagc
     4021 agcagccatt gcttctacat cgactgccac ccgattcgtt ggattagcca ctagacgctc
     4081 aaaatgagtt ggcaacggca agataaaccc ttcgtttgcc tcttggctag ttgagcgcag
     4141 atgcttcaga tctgaagttg cctgggctaa gtcagaggtt gatttgcaat cagcataggt
     4201 attccacagt gacatgaagc cgctattttt taattctgtc aattgaggtg taggaccggt
     4261 tgtacaacct gcagtaagaa gtgctgctcc aaataagccc acgaccaacc cttctctgaa
     4321 tgcatcacag ttcatccggc tatactcccc cctaacaact tctgatatcc actgcaaatg
     4381 gcaggcctga ggagcatcgc gtggaatcgc acttaaaacc agagagtttt gtgaacttga
     4441 ccggcaggtg atgtgtcaat ctccactcac tgttgcatca ttgacacaac ggaaagatgt
     4501 cgaaatatca gtatcttgga caaagatcat caagaattga agatttggac aagaattcca
     4561 gtacgggtgg tagcgttggt ctttcgcatg atgtgcttaa tgtgctcctt tacggtttgt
     4621 tcagtaatct ggagggcatt ggcgatttct ttattggtcc acccttttgc caaatgttca
     4681 acgactgatt gctcgcggtt agtcaactga aaacgttctc gagcatgttc tgtattgagg
     4741 ttttgtttcc tccccaactc ttccatggta accacaagcc tggcgttttg gatgcctcct
     4801 cgatccggta agccaaagcc tcttagaagg ataggctgat taggatcgcc tgtcacgcgt
     4861 ttgagttcaa attgttccca atccttggct tccgtgcgta tgtgaagggc tttaataatt
     4921 tccgcacaaa gctcagtcag agccgtcggg aggactccat gcgctgaaat tgtggtagtt
     4981 ctcccatgct cgacggcgtt gatctttttg gcaagctccg tagcttgtcg attcatatgt
     5041 agaagttgca tagatgccga cagcaccact atcccggaac cggccctctg atcagcaagg
     5101 gcatctgttt ggtcgatacc tttaactgct tctgtcataa cttaagacat cacaacattg
     5161 tctgaatttc gggtctgaca gtaatcgcat agctgacgga acaagcattg ggataatgcg
     5221 gggatagtac ctaacccttt cgagggagtc aacatatacc tttgagaaag cagcctacgt
//
LOCUS       DATNDD010000281         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3491730, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000281 DATNDD010000000
VERSION     DATNDD010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3491730"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VN647_06725"
     CDS             complement(<1..403)
                     /locus_tag="VN647_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96761.1"
                     /translation="MYKLHLILKYLLKRRIAWVSLAAVMLCTTMVLVVISVMGGWLNM
                     FKTSFRGLSGDIVVHSVSLKGFPYYEKIIAAIKKDPDCQVEAALPSIETAGLFNSGTG
                     PIYARVMGIPIEQMHTVNKFDESLYRQHTAIE"
     gene            complement(524..928)
                     /locus_tag="VN647_06730"
     CDS             complement(524..928)
                     /locus_tag="VN647_06730"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWG96762.1"
                     /translation="MSVPENIRMDSLWKMKAYRLGLFLSDLAWRDVTRLVQDRRTIEI
                     ADQLYRAVCKISSNVGEGYSRNTGKDRARFYEYALGSAREARDWYYKARHILDEAVNA
                     HRAELTTEIIRLTLTMVARERRIGRKFSDTDE"
     gene            complement(1003..1242)
                     /locus_tag="VN647_06735"
     CDS             complement(1003..1242)
                     /locus_tag="VN647_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96763.1"
                     /translation="MKNSTKAKKPFWELTPQERERETKKYDKPIPLSKTKPLTKRERE
                     QWERMRKAPHVSVYVRRGPDETIARLDPEVRPRKK"
     gene            complement(1369..>2459)
                     /locus_tag="VN647_06740"
     CDS             complement(1369..>2459)
                     /locus_tag="VN647_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWG96764.1"
                     /translation="SEVVAEIRRYMTDRGYVEVETPMMQPIPGGAAARPFITHHRALD
                     IDLYLRIAPELYLKRLLVGGFSKVFELNRNFRNEGISPRHNPEFTMLEAYEAYGSWET
                     MADLVEGMICHVAEKLFGGLKIEHKNAEGVVTKTIDLTRPWRRVSMVDLVAEKTRDRY
                     GAEFQWRFDKRSLHEANPALFEKMTTEHRDLRTSEVYGAQGRPQPKLLQEIQENERKY
                     FANKLAQLSPAEQLQEVYEKLIEPTLIDPTFVTHVPSVIIPLARKWKEDPFFADVYEL
                     AINGQEISPGYTELNDPDVQADNFKHQVGDKEEQQKTDEDFLNALRYGMPPAGGMGLG
                     IDRLVMMLTGAESIRDVILFPLMKPTGE"
BASE COUNT          492 a          696 c          659 g          612 t
ORIGIN      
        1 cttcaatggc cgtgtgctgg cgatagaggg attcgtcgaa tttgttgacg gtgtgcatct
       61 gctcgatcgg aatccccatc acccgcgcat agatcggccc agtcccagag ttaaacaacc
      121 ccgccgtctc gattgaggga agggccgcct ccacctggca atccggatcc ttcttgatgg
      181 ccgcgatgat cttctcgtaa tacggaaatc ccttgaggct gaccgagtgc accacaatat
      241 ccccgctgag cccgcggaag gaggtcttga acatattgag ccacccgccc atgacactga
      301 tgacaaccag caccatcgtg gtgcagagca tcacagcagc gagggagacc caggcgatgc
      361 ggcgcttgag gaggtacttg aggatgaggt ggagtttgta catctggccg atgaatgagt
      421 aatgcgggaa tcaatgagta atgactaaaa cgtaacaagg caaggcagtc gcggttccta
      481 cgaacaggcg acggaacaaa gggtgacgac taagatgtaa tgattattcg tctgtatccg
      541 agaactttct tccgattcgg cgctcgcggg ccaccatggt gagggtaagg cgaatgatct
      601 ccgttgtcag ttccgcgcga tgagcattga cggcttcgtc aagtatatgc cgagccttgt
      661 aataccagtc gcgggcttca cgggctgaac ccaaggcgta ctcatagaac ctcgcgcgat
      721 cctttcccgt gtttcgtgaa tatccctcgc caacgttcga gcttatcttg cagacggcgc
      781 gatacagttg gtcagcgatt tcaatcgtcc gacgatcttg caccagacgc gtcacgtcac
      841 gccaggcgag atccgacaag aacaatccga gccggtacgc cttcattttc caaagcgaat
      901 ccatccgtat attttcggga acactcattt cccattctgt atacgtcatc ggccaccacc
      961 gatctctcac gcgcagacgc ctctttacgc tctagtcatt gctcattttt tccgcggacg
     1021 aacctccgga tcgagtctcg cgattgtctc gtcgggaccg cgtcgtacat agactgaaac
     1081 atgcggggcc ttacgcatgc gttcccattg ctcccgttca cgtttggtca acggctttgt
     1141 cttcgacaaa ggaatcggct tgtcgtactt cttcgtctcg cgctcacgct cttggggcgt
     1201 cagttcccaa aacggctttt ttgctttcgt cgaatttttc atgcatcagc ttttctccgt
     1261 gtcgccgccg gtagggtccg cgcggacgcg ttcgagattc acggaaccgc agattgcttc
     1321 cctcaatgcg agacgttcct ctcttgttac gttttagtca ttactcattt attcccccgt
     1381 cggcttcatc aacggaaaga gtatcacatc gcgaatactc tccgcgccgg tgagcatcat
     1441 cactagccga tcgattccga gtcccatccc ccccgccggg ggcattccgt atcttagggc
     1501 gttgaggaaa tcctcgtctg tcttttgttg ttcttccttg tcgccgacct ggtgtttgaa
     1561 gttatcggcc tggacatcgg ggtcgtttaa ctcggtgtag ccgggggaga tttcctggcc
     1621 gttgatcgcc agctcgtata cgtcggcgaa gaagggatcc tccttccact tgcgggccag
     1681 tgggatgatg acggagggaa cgtgggtgac gaaagtgggg tcgatgagcg tcggctcgat
     1741 tagtttttcg tagacttctt gcagttgttc ggcaggcgaa agttgcgcga gcttattcgc
     1801 aaaatacttc cgttcgttct cttgtatctc ctgcaacaat ttgggctgtg gacgaccctg
     1861 cgcgccataa acctctgaag ttcgcaagtc ccggtgttcg gttgtcattt tttcaaataa
     1921 ggcgggattg gcctcgtgga gggaccgctt atcaaatctc cactggaatt ccgcaccgta
     1981 ccgatctcgc gtcttttctg caacgagatc gaccatcgac acccttctcc acggcctcgt
     2041 cagatcaatc gtcttcgtca ccaccccttc cgcgttctta tgctcaatct tcaacccacc
     2101 aaatagtttt tccgccacat ggcagatcat cccttccacc aaatccgcca tggtttccca
     2161 cgacccgtag gcttcgtagg cttccagcat ggtgaactcg gggttgtggc ggggactgat
     2221 gccttcgttg cggaagttgc ggttgagctc gaagactttg ctgaatccgc cgacgagcag
     2281 gcgtttgaga taaagctcgg gggcgatgcg gagatacagg tcgatgtcca gggcgcggtg
     2341 gtgcgtgata aaggggcggg ccgctgcgcc gccggggatt ggctgcatca tcggggtttc
     2401 gacttcaaca tagccgcgat cggtcatgta gcgccggatc tccgccacca cctcgctgc
//
LOCUS       DATNDD010000282         9443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2059270, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000282 DATNDD010000000
VERSION     DATNDD010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059270"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="fabF"
                     /locus_tag="VN647_06745"
     CDS             <1..897
                     /gene="fabF"
                     /locus_tag="VN647_06745"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWG96765.1"
                     /translation="EHYHDVLKGKGPGRVSPFFIPMTIINLASGQVAIRIGAKGPNSC
                     AVTACATGNHCIGDAYRLIQRGDADVMVAGGAEAAVTPLGVAGFAAAKALSFRNDQPT
                     KASRPFDKDRDGFVLGEGAGVVVIEELEHARRRGIRIYGEIIGYGLNSDAYHITAPPE
                     EGEGAVRCMELALKDAGITRDQIGYINAHGTSTMADAIETRAIKRVFGEQAFRIPVSS
                     TKSMTGHLLGAAGGIEAVFSLLALFHGILPPTINLDNPDPACDLDYVPNKARPAAVTT
                     ALSNSFGFGGVNACLIFKRLDA"
     gene            911..1618
                     /gene="rnc"
                     /locus_tag="VN647_06750"
     CDS             911..1618
                     /gene="rnc"
                     /locus_tag="VN647_06750"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWG96766.1"
                     /translation="MTPASSADSFPAISNYRFTNPNFLTEALTHKSYVNERRDSGRKH
                     NERLEFLGDAVLSLIVSDYLARRYPELSEGALSKLKAKLVSEAPLANAARRLDLGARL
                     KLGRGEELSNGRDKASLLADAFEAIIAAVYLDGGLEASRSFTIEALTDDLRRIDILQE
                     KPGGDDYKTRFQEWCQKRYEVLPRYVIVRETGPDHQKLFEVEVHVNDRALGIGRGYSK
                     KEAEQEAAQRALEQSER"
     gene            1676..2281
                     /locus_tag="VN647_06755"
     CDS             1676..2281
                     /locus_tag="VN647_06755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWG96767.1"
                     /translation="MLKHAGRRMLMGVLIAVGLSVSSVGSIGAAETTQPPKAETPKGP
                     KAIIKTKFGDIEIKFYPDVAPKHVENFIKLAKSGFYNGTIFHRVIPGFMIQGGDPNTK
                     DTLKRDIYGQGGPGHAIKAEFSDIPHKRGIVSMARAADPDSAGSQFFIVVEDSRFLDR
                     KYSVFGEVTKGIGVADKIVNLARDERDNPKERVEMTVTIVE"
     gene            complement(2325..3227)
                     /locus_tag="VN647_06760"
     CDS             complement(2325..3227)
                     /locus_tag="VN647_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWG96768.1"
                     /translation="MSLPDSLNVPILRNIFHPSDFTPASEAAFAHALRAALMAQANLT
                     ILHVSPDRDGEWMEFPGVRAALERWGLLPMNSRRSDVAQLGIHVKKVRAIHPDPIESV
                     TSYLAEHATDLIVLATDQSNGCLPWLSKSVAVPVALESRQMTLFIPKGAAGFVSSHDG
                     SVSLTNILIPVASKPPGQAALQAAVRMAYRLNRSVGVFTVLHVGEEGDMPDLHYSDMP
                     GWQWEKVIKQGDVVDAICGIAEEIKADLIVMTTEGRHGFLDALRGSHSERVLHKTPCP
                     LLTIPAGGFIASVLEEESESRQAP"
     gene            complement(3412..4113)
                     /locus_tag="VN647_06765"
     CDS             complement(3412..4113)
                     /locus_tag="VN647_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96769.1"
                     /translation="MSSCTYCHHRKGKRPCPALGGLICSLCCGEHRLTRISCPNDCTY
                     LDSGNDYQQKRLGVQFAPVRRDFYQELDRLGGHKAVTFFNLIEVITFGYFEGRRDGQD
                     VEVITALQTLRRALSPLHVPAGPAPVFAEHLKKEYELFRKQNPEEMADASDALVVLDR
                     AAQFVSAFSENDFQSSRFLGGVIGYVKMEHPEIAAHLKKQREAGHILLPGQSFLPTPS
                     AETHTHGPDCRHHHN"
     gene            complement(4110..5153)
                     /locus_tag="VN647_06770"
     CDS             complement(4110..5153)
                     /locus_tag="VN647_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96770.1"
                     /translation="MTLSLFLRYDGSGGKLDILPLFVKLYHRLFLSTALILGLAFPGK
                     AAQITGLESPNSFVGDPSGKEYFISNINGEPDARDNNGFITKLNAEGKIIDLKFIQGG
                     VADVLLHAPKGMALVGPILYIADLDQLKAFDKASGKLFATVSFPTSPNMRVSLTDVAA
                     APGGVLYVTDQAANAIYRIDPSADHRVDLLIHDERLAGPAGIAIHPGTNHLITVSWEK
                     GKILDITPGGQLTELESNGFFTGRFQNLSGVDFDQWGNMYVSDLTKGKIWRMTRDHRF
                     QVIAEYLPAPADISIDRVNNLILVPYHYMHAAEINGLETPSSAKPKGEKRTLADYGFI
                     PPPPKPGSEGTKK"
     gene            5280..6140
                     /gene="folD"
                     /locus_tag="VN647_06775"
     CDS             5280..6140
                     /gene="folD"
                     /locus_tag="VN647_06775"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HWG96771.1"
                     /translation="MAAQLIDGKTLAQQVRDRLTKESTELFVKKAMKPGLATILVGDD
                     PASHVYVRNKQKACELAGIYVDDHKLPASTTQVELLALIDKKNTDPKIHGILVQLPLP
                     KHIDSKVILESVSPLKDADGFHPYNFGRLVEGHPVFEACTPKGVIKMIESTGMTIEGK
                     RAVVVGRSNIVGKPLALMLLQRNATVTICHSKTKDLPGVCREAELLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKTSEGKLCGDVDFDQVSQKAGWISPVPGGVGPMTIAMLLENTV
                     ESAKRAAGML"
     gene            6152..7039
                     /locus_tag="VN647_06780"
     CDS             6152..7039
                     /locus_tag="VN647_06780"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWG96772.1"
                     /translation="MSREPQRLIDVLNRGTFAVTVEYNPPKGTNISSVLESAKQLVGR
                     VHGVNVTDNTAAVVRAGSLPVCRLLYELGHDPVMQLTCRDRNRIAMQSDLMGGHMLGI
                     RNILCLTGDYPTVGDHKEAKPVYDLDSVQVMQLVQGLNKGHDLAGHKLDGSTAFTIGA
                     AVTPEADPLGPMLAKFEVKVKAGAQFFQTQAVYHPEQFASFMKAVQPYKVKVLAGILV
                     LRNHKMAEFMNGNIPGISVPGEMIDELKAAGEKAEDVGVDIAVRTIKAVRPHCDGVHI
                     MAIKATHRLAEIITKAELN"
     gene            7039..7827
                     /gene="panB"
                     /locus_tag="VN647_06785"
     CDS             7039..7827
                     /gene="panB"
                     /locus_tag="VN647_06785"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWG96773.1"
                     /translation="MTILDFQQFKRDRKKLAVVTAYDALFARIVEQAGIRVILVGDSL
                     GMVVQGKPNTLAVTMDDMLYHTRLVAGVVQRALVIADMPFMSYQTSTEEALRNAGRLL
                     QAGAAAVKLEGGAVMVDRIKAMTSVGIPVMGHLGMTPQSVYALGGYKVQGKADDQASR
                     LLQDAKAIEAAGAFALVLEAIPADLARKITHTLSISTIGIGAGPHCDGQVLVLYDLLG
                     LFDAFIPKFVKTYAHLKADTLQALSRYKEEVEQGKFPSDSESYH"
     gene            7965..8741
                     /locus_tag="VN647_06790"
     CDS             7965..8741
                     /locus_tag="VN647_06790"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWG96774.1"
                     /translation="MDWVDWLLSQEVRQFVFDHHLVYPLVFVLRLFGVTALELLRPAR
                     TIPYRRLIGRDILLISIYWLGMVPIAESIDRLIAIRPHLPQAVHEMPLLLRLFCYFLI
                     ADFGHYWIHRLMHMKHVWRIHKWHHSPTHMYWLAGFRATIPQDVIVNLPYLFAYSFLD
                     LSPSWMELAIGLFNNLQNDWMHANIVWRSNRLEWLIVTPRYHHIHHSDKPAHYMANLG
                     TLFTVWDRLFGTYVDPESVKGELSFGIGEKVPPLRLVLGV"
     gene            complement(8757..>9443)
                     /locus_tag="VN647_06795"
     CDS             complement(8757..>9443)
                     /locus_tag="VN647_06795"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HWG96775.1"
                     /translation="DSLGVLRYIRSLGPSARVVLGNHDLFLLAVSEGAAALRPKDTIG
                     DVLKADDRPDLIDWLRHQPLHQREGLFFMIHAGLLPQWTTQDAAQLACEVETALRGDG
                     FQSFLRSLFHEPAVTWSPDLTGASRLVTVARILTRLRACTPTGDTLNFSGPPAEIPPG
                     YFPWFHLPARRSADTTIITGHWAALGLHMEPNLLAIDSGCVWGRQLTAVRLEDRRMFQ
                     VNCVDAVRRA"
BASE COUNT         2128 a         2447 c         2784 g         2084 t
ORIGIN      
        1 gagcactatc atgatgtgct caaaggcaag gggcccggcc gggtctcacc gtttttcatt
       61 cccatgacca tcatcaattt ggcgtccggg caggtggcga ttcggatcgg agccaagggg
      121 cccaattctt gtgcggtaac ggcttgcgcc acgggcaatc attgcattgg ggatgcgtac
      181 cgcctgattc aacgaggcga tgccgatgtc atggtggccg gcggtgccga agcggcggtc
      241 accccacttg gtgtggcggg attcgcagcg gccaaggcat tgtcgttccg gaatgatcaa
      301 ccgacgaagg cgagtcgccc gttcgacaag gaccgagatg gatttgtgct gggcgagggc
      361 gcgggggtgg tggtgattga ggaattggaa catgcccgtc ggcgtgggat cagaatctac
      421 ggcgagatca tcgggtatgg gctgaacagc gatgcctacc acattactgc cccaccggaa
      481 gaaggcgagg gggccgttcg ttgcatggaa ttagcgctca aagatgcagg aatcactcgc
      541 gatcagatcg ggtatattaa tgcgcatggt acgtcgacaa tggccgatgc cattgagacc
      601 cgagcgatca aacgggtgtt cggggaacag gcgtttcgaa ttccggtcag ctcgaccaaa
      661 tccatgaccg gacatctact cggagctgcg ggagggatcg aagccgtctt cagtcttctg
      721 gcgctgtttc acggtattct cccccccacg atcaatctgg ataatccgga tccggcctgc
      781 gatttggact atgttcccaa taaggcacga cctgccgccg ttacaacggc gttgtcgaac
      841 tcttttggat tcggcggagt gaatgcctgc ttgatcttca agagattgga cgcgtagtgc
      901 gcgtatcgcg atgacgccgg cttcttcagc cgattctttt cctgccatat cgaactatcg
      961 attcacgaat ccgaacttct tgacagaagc attgacccac aagtcgtacg tgaatgagcg
     1021 ccgagactcc ggtcgaaaac acaatgagcg gcttgagttc ttgggagatg ccgtattgtc
     1081 gctcatcgtg agcgattatc ttgcgaggcg gtatcctgag ttgagcgaag gagccctttc
     1141 aaaactcaaa gccaaactcg tgagcgaagc gccgttagcg aatgccgcca gacggctgga
     1201 tctcggcgcc aggttgaaac ttggtcgagg cgaggaactc tcgaatgggc gggacaaagc
     1261 ctcactgttg gctgatgcgt ttgaggccat cattgcggcc gtctatcttg atggaggttt
     1321 ggaggcgagt cgaagcttca ctattgaggc gctgaccgat gacttacgcc gaatcgatat
     1381 cctgcaagaa aaaccagggg gagatgatta taagacgcgc ttccaggaat ggtgtcaaaa
     1441 acggtatgaa gtgttgcctc ggtatgtaat cgtacgcgaa accggtccgg atcaccaaaa
     1501 gttatttgaa gtggaagtgc atgtgaacga tagggctttg gggatcggcc gaggatacag
     1561 caagaaggaa gcggaacagg aggcggcgca acgagcgctg gaacagtctg agcggtaatg
     1621 ccgagtccat gctacaatga tcgacactgt cacatgaaag acatgggagg aagtaatgct
     1681 gaagcacgca ggacggcgaa tgcttatggg cgtgttgatc gcggtcggtc tatcagtctc
     1741 aagcgtcggg tcgatcggtg ccgctgagac gacacaaccg ccaaaggctg aaacaccgaa
     1801 agggccgaag gcgatcatca aaacgaaatt cggcgacatc gaaatcaagt tctatccgga
     1861 tgtcgcacca aaacacgtgg agaattttat caagttagcg aagtccggat tttacaatgg
     1921 aacaattttt caccgtgtca ttcccggttt catgattcaa ggcggagatc cgaatacgaa
     1981 ggacacactt aaaagggaca tctatgggca gggtggcccc ggccatgcca tcaaggcgga
     2041 attcagcgat attccgcaca aacgcggcat tgtttcaatg gccagggcgg ctgacccgga
     2101 ttcggccggc tcccagtttt ttatcgtcgt cgaggactca cgatttctgg accgcaagta
     2161 ctccgtgttc ggcgaagtga cgaagggtat cggtgtggcc gacaagatcg tcaatctggc
     2221 acgcgatgag cgggataatc cgaaagagcg ggtggaaatg acggtcacga tcgtggagta
     2281 gtttccctcg atatttgtgc tggaggctca tgtgcggcgc gtcttcaagg tgcctgtcgg
     2341 ctttctgatt cctcctccag caccgaggca ataaatccgc ctgctggaat tgtcagcagc
     2401 gggcagggcg tcttgtgaag cacccgctcg ctatggcttc cccgtagcgc atcgagaaaa
     2461 ccatggcgtc cttcggtcgt catgacgatg agatccgcct tgatctcctc agcgatcccg
     2521 cagatcgcat ccaccacatc tccctgcttg atgactttct cccactgcca gccgggcata
     2581 tcggagtagt ggaggtcggg catgtcccct tcctcgccga catgtagaac cgtaaagacg
     2641 ccgaccgagc gattgagccg gtatgccatc cgcacggcgg cctggagagc cgcctggcca
     2701 ggcggcttgg aggcgacggg aatcaagatg ttcgtcaacg agacggaacc atcgtgcgag
     2761 gaaacgaatc cggctgcacc tttcgggatg aagagggtca tctgtcgcga ctccagcgca
     2821 accggcaccg ccaccgactt gctgagccat ggaagacagc cgttgctctg atcagtagcg
     2881 agcacgatca aatccgtcgc gtgttcggct aagtaagatg tgactgattc aatgggatcc
     2941 ggatggatcg ctcggacctt cttgacgtgg atacccaatt gggcgacatc ggatcggcga
     3001 ctgttcatgg gcagcaggcc ccatcgctcc agcgccgcac gtacaccggg aaactccatc
     3061 cattccccat cccgatcggg ggaaacatga aggatggtca ggttggcttg agccatcaag
     3121 gcggctctca aggcatgagc gaacgctgct tcgcttgcag gagtaaaatc ggatggatga
     3181 aaaatattcc gaaggatagg gacgttgagc gagtcgggaa gagacatagg tggtccccct
     3241 cggacggata gcatatcatg caggtgcttc gacttgcact ggggaagtcc ccgggctgat
     3301 ggacgggttc gcgcccgctg tcttccgtgt ataggcctgc tcctagatgg gggtcagcct
     3361 tgcaccggcc ggcaatttcc ttgagcgatc gggcgctgca ccataagtga gctaattatg
     3421 atggtgtctg cagtcgggac catgagtgtg agtttcggcg gaaggtgtcg gcaggaaaga
     3481 ttgtcctggt aaaagaatgt gtcccgcctc acgttgcttc ttgagatggg ccgcgatctc
     3541 cggatgctcc atttttacat aaccgatcac ccctccaaga aatcggctcg actgaaaatc
     3601 attctctgaa aatgcggaga caaattgcgc cgcgcgatcc aggacgacca gcgcgtcgga
     3661 ggcgtcggcc atttcttcgg gattctgttt ccgaaacaac tcatactcct ttttgagatg
     3721 ctcagcaaaa accggtgcgg ggccggcggg gacgtggagt ggactgagag cgcgacggag
     3781 cgtttgaagt gccgtgatga cctcgacatc ttgtccatcc cttcgcccct caaagtagcc
     3841 gaacgtgatg acttcaatca gattgaagaa cgtcacggct ttgtggcctc ctaagcggtc
     3901 cagttcctga tagaagtctc gccggacggg cgcaaattgc acaccgagtc ttttttgttg
     3961 gtaatcgttt ccgctgtcga gatacgtgca atcattgggg cacgagatcc gtgtcaggcg
     4021 atgctcccca cagcagagac tgcagatcaa tcctccgagg gcagggcacg ggcgtttgcc
     4081 ctttcgatgg tggcaatacg tgcacgaact catttcttcg ttccttccga cccgggcttg
     4141 ggaggcgggg gaatgaatcc gtaatcggct agtgtgcgtt tctcgccctt aggctttgcg
     4201 gatgaagggg tttcaagtcc atttatctcg gcggcatgca tgtagtggta gggaaccaag
     4261 atcagattgt tcacgcggtc gatactgatg tcggccggag cggggagata ctcggcgatg
     4321 acttggaacc gatggtctcg ggtcatgcgc caaatcttgc cttttgtgag atctgataca
     4381 tacatgtttc cccattgatc gaaatccact ccgctcaggt tttggaagcg accggtaaaa
     4441 aacccattcg attccagctc ggtcagttgt ccgccaggag tgatgtcaag aatcttccct
     4501 ttttcccagc tcacggtgat gagatgattc gtcccgggat gaatcgcgat tccggctggt
     4561 cctgcgagcc gttcatcatg gatcaatagg tcaactcgat gatcggccga cggatcaatc
     4621 cggtaaatgg cgttcgccgc ttggtctgtc acgtacaaga ccccgcctgg cgcggccgcc
     4681 acgtcggtca gtgatacccg catattgggt gatgtcggga atgagacagt ggcgaaaagt
     4741 ttgcctgacg ccttatcaaa agctttcagt tgatcgagat ctgcgatata aagaatcggt
     4801 cccaccaaag ccatcccttt cggggcatgc agcaacacgt ccgccacgcc gccttggatg
     4861 aacttgaggt caatgatttt tccctcggca ttcaattttg tgatgaaacc attgttatcc
     4921 ctggcgtccg gttcgccgtt gatgttggaa atgaaatatt ccttcccgga cgggtcgccg
     4981 acgaagctgt tgggtgactc caaaccggtg atttgcgcag ctttgccggg gaacgccaag
     5041 cccaggatga gagcggtaga cagaaataag cggtgataga gtttcacgaa caaaggcagg
     5101 atgtccaatt ttccaccgga gccatcgtat cggaggaaca aagaaagtgt caagaaagga
     5161 gccctggagg ttttgacgtc aagaagagag aacggagaga gcaaagcgtt gacttgcata
     5221 aaattcccct gctaagttgc ggcgatttag cccggcagaa tagttatagg gaggggatcg
     5281 tggccgcaca attgattgat ggaaaaacgc ttgcgcaaca ggttcgcgat cgtctgacaa
     5341 aagaatcgac ggaactgttc gtcaagaaag cgatgaaacc tggtcttgcg accattttgg
     5401 tgggtgacga tcccgcttcg catgtgtatg ttcgaaacaa gcaaaaggcc tgcgaattgg
     5461 caggtatcta tgtcgacgat cacaaactcc cggcgagcac gacacaggtc gagttattgg
     5521 cgctgatcga caaaaaaaat actgacccca aaatccatgg aattctggtt cagctcccgc
     5581 tgcccaaaca catcgacagc aaggtcattc tagaatccgt ctctccactc aaggatgcgg
     5641 atggatttca tccgtataac ttcggccgac tggtggaggg gcaccccgtc ttcgaagcct
     5701 gcactcccaa gggggttatc aagatgatcg agtcgaccgg gatgacaata gaaggcaagc
     5761 gggcggttgt ggtgggacgg agcaatattg tcggcaagcc gttggcgttg atgctgcttc
     5821 aacggaacgc aacggtcacg atctgtcatt caaaaacgaa ggacctgcct ggggtttgcc
     5881 gtgaagcaga gttgctcctc gttgcgatcg gaaaggcgaa atttgtcact gccgacatgg
     5941 tgcgcgaagg tgcggttgtc atcgacgtgg ggactaacaa gacttctgaa ggcaagttgt
     6001 gcggcgatgt agattttgac caggtcagtc agaaggccgg ttggatcagc cctgtccccg
     6061 gtggggtcgg ccccatgacc attgcgatgc tgctggaaaa tacggtggag tcagccaaga
     6121 gggcagcagg aatgctatga gggggccatc tatgagtcga gagccacagc gcctaatcga
     6181 tgtcctcaac cgagggacgt ttgccgtcac ggtcgagtat aaccctccca agggcaccaa
     6241 catctcgtcc gttctcgaga gtgccaaaca gcttgtcggg cgcgtacacg gcgtgaacgt
     6301 cacggacaat accgccgcgg tggttcgtgc cggctcactt ccggtctgcc ggctcctgta
     6361 tgaattgggg cacgaccctg tgatgcagtt gacctgtcgt gatcgcaatc ggatcgccat
     6421 gcagtcggat ctgatgggcg ggcatatgct tgggattcga aatattctgt gcctcacagg
     6481 cgattatccg acggtcggtg atcataagga ggccaagccg gtgtatgatc tcgattcagt
     6541 ccaagtcatg caactggtgc aaggattgaa caaggggcat gacctggccg ggcacaagct
     6601 ggacggctcg acggcgttta cgatcggtgc cgccgtcaca ccggaggccg acccactcgg
     6661 acctatgctc gccaagtttg aagtaaaggt aaaagcgggc gcccaatttt ttcagacgca
     6721 agcggtgtac catccggagc agttcgcaag tttcatgaaa gcggtccagc cttataaggt
     6781 caaggtcctt gccggtattc ttgtattgcg caaccacaag atggcggagt tcatgaacgg
     6841 gaatattccg ggcatctcgg ttccaggcga gatgatcgat gagctcaaag ccgccggtga
     6901 gaaggcagag gacgtcggag tcgacatcgc cgtgcggacg atcaaggcgg ttcggccgca
     6961 ctgcgacggt gttcatatta tggcgatcaa agccacgcat cggctggccg agatcatcac
     7021 caaagctgaa ttgaactgat gacgatcctc gatttccagc agttcaaacg agacaggaag
     7081 aaactcgccg tcgtgacggc ctatgacgcg ctgttcgcac gtattgtgga gcaagcggga
     7141 atccgggtga ttttggtcgg ggattcgctg ggcatggttg tacagggaaa acccaatacg
     7201 ctcgcggtca cgatggacga catgctgtat cacaccaggc tggtcgccgg cgtggtgcag
     7261 cgggctctgg tcattgcgga tatgccgttt atgtcctatc agaccagcac agaggaggcc
     7321 ctgcgcaatg cgggtcgatt gctgcaagca ggtgccgctg cggtgaagct ggaagggggg
     7381 gccgtgatgg tagatcgaat caaagccatg acgagtgtcg gtattcctgt catgggccac
     7441 ctaggtatga caccacaatc agtctatgcg ttgggcggat acaaggtcca aggtaaagcc
     7501 gacgatcaag cttctaggtt gctccaggat gcaaaggcca ttgaagctgc cggagccttc
     7561 gcgcttgtat tggaagccat acctgctgat ttggctagaa aaatcaccca caccctttca
     7621 atctccacga tcgggattgg agcgggacct cactgtgacg gccaagtgct ggtgctctat
     7681 gatctccttg gactctttga tgcgttcatc ccgaaattcg tgaagaccta cgcccacctc
     7741 aaagccgaca ccctccaagc gctgagtcgt tataaagaag aggtggagca aggtaaattc
     7801 ccgagcgatt ccgagagcta ccactagctc ctctttccct tgcgatgggg ttaggtcgct
     7861 cactcatcgt gcgcgcttgc cagatccgat tcgtcaattt ccacagaaag cacgacaggt
     7921 cataccgaat acgataaggt gcgccataaa gagggagcgc cttgatggat tgggttgact
     7981 ggctcttgag tcaggaagtt cggcagtttg ttttcgacca tcatcttgtc tatccactcg
     8041 ttttcgtcct gcgtctgttc ggcgtgaccg ctcttgaact gcttcggcct gcgcgaacga
     8101 tcccctatcg taggctcatc gggcgggata ttctcctgat ctccatctat tggttaggca
     8161 tggtcccgat cgccgagtct atcgatcggc ttatcgccat ccgcccgcac ttgcctcagg
     8221 ccgttcacga gatgccgttg ctgcttcgcc tgttctgcta cttcctcatc gcagatttcg
     8281 ggcattactg gattcatcgg ctgatgcaca tgaagcatgt ctggcggatt cataagtggc
     8341 accattcgcc cacccacatg tattggctcg cgggctttcg cgcgacgatc ccgcaagatg
     8401 tcatcgtcaa tctgccctat ctgtttgcct attcattctt agacctctct ccttcgtgga
     8461 tggaacttgc gatcggcctc ttcaacaatc tgcaaaacga ttggatgcat gccaacattg
     8521 tgtggcgctc caaccggctc gaatggctga tcgtgacccc gcgctatcac cacattcatc
     8581 acagcgataa gcctgcgcac tacatggcca atcttgggac gttgtttact gtctgggacc
     8641 gtttgtttgg gacttatgtg gatccggagt cagtgaaggg agagctttcg ttcgggattg
     8701 gagagaaggt gccacctctt cgccttgtat tgggtgtcta gaatatgtac gtgagcctac
     8761 gctctgcgta ccgcatccac gcagttcacc tgaaacatcc tgcgatcttc caatcgcacc
     8821 gctgtcagtt gccgtcccca cacacaaccg ctatcgatgg caagcagatt gggctccatg
     8881 tgtaggccga gtgcagccca atggccggta atgatggtcg tatccgcact gcggcgagcg
     8941 gggagatgga accaagggaa atagcccggc ggtatctctg ccggcggacc ggaaaagttc
     9001 aaggtgtcgc cggtcggtgt acaggcgcga agtcgggtta atatacgggc aaccgtgacc
     9061 aaccgtgagg cgccggtaag gtcggggctc cacgtgaccg ccggctcatg gaagagtgat
     9121 cgcaagaacg attggaagcc gtcgccgcgc agtgcggtct cgacctcgca ggccaattga
     9181 gcggcatctt gcgttgtcca ttgggggagt agccccgcat ggatcataaa gaataaccct
     9241 tcgcgctggt gcaggggttg gtggcgaagc cagtcaatca agtccggccg atcgtcggct
     9301 ttcaatacgt cgccgatcgt atctttgggg cgaagcgccg ccgccccttc cgagactgcg
     9361 agaagaaaga ggtcgtggtt gccaagcacg acgcgtgcgg atggtccgag cgaacggatg
     9421 tagcgaagca caccgaggga atc
//
LOCUS       DATNDD010000283         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_329619, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000283 DATNDD010000000
VERSION     DATNDD010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329619"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1471
                     /locus_tag="VN647_06800"
     CDS             92..1471
                     /locus_tag="VN647_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWG96776.1"
                     /translation="MGKSLPIFNGPSDTFSSLQQQVAQFFTPTPKGDGPFDGRTVDDF
                     KPYLVALNLTKRCNLKCDHCYLDATTKAAGGDDELSTEECYRLIAQIAEVNKGCLLVI
                     TGGEPFVRPDILDIARYAVKLGFMVVFGTNGMLINDQLAKTLVEIGVMGVGISIDSLQ
                     AAKHDAFRGVPGAWEAAVAGIEASKRNGLQFQVHFSAQPMNYTELPEVVAWAHRLGAR
                     VLNVFFMVCTGRGEELTDITPAQYEEVLGYLIECQDNYKGMLVRARCAPHFKRLAYEK
                     DPNSPITKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRHQSFGDLWERSD
                     VFNSFRYPQLKGKCGDCEYSDICGGCRARPYVDHGDWLDEDQWCLYTPKGGEKIKVAF
                     NVSEETDVTWDDASALRLSRIPYFLRAMVKKGVEKHARENDVRLITVELMEELRKKRF
                     GNDAPVFKF"
     gene            1570..2079
                     /locus_tag="VN647_06805"
     CDS             1570..2079
                     /locus_tag="VN647_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HWG96777.1"
                     /translation="MDTLQSITTDLNILIPIINHWFHLLSAVIWIGGLAFLVMAVTPG
                     LQKAVPRDQIKPITDIFYRYYKKVAGILLVVLLFTGGINLHYVNQVLVSQSGNGVEHH
                     SKYLMVFMIKLLLVLGLLTLFLYTVIFRSEDETDEEEPYEAIPFQRAALWMGFFIILC
                     AAAMKHLHQ"
     gene            complement(2764..>3207)
                     /locus_tag="VN647_06810"
     CDS             complement(2764..>3207)
                     /locus_tag="VN647_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWG96778.1"
                     /translation="TKLVYCPDNDPHSCALYYYTEPGDHIGFHYDTSYYKGARYTILM
                     GLVDRSTQCKLVCELFKDHPTKQPRHLELITEPGDMVIFNGDKLWHAVTPLGEGEERI
                     ALTMEYVTNPEMGTVKRLYSNLKDSFGYFGFKSVFKRALGLSRFK"
BASE COUNT          741 a          857 c          831 g          778 t
ORIGIN      
        1 ttcctagttc tgggtgctga gtcctgagag aaattagaat tgctaggcgg gcaactcagg
       61 actcaactct cagcactcag cactagtagt catgggtaaa tctcttccta tcttcaacgg
      121 cccttccgac acttttagtt ctttgcaaca acaggttgct cagttcttca cacccactcc
      181 aaagggagac ggaccgttcg acggtcggac ggttgatgac tttaagccct acctcgttgc
      241 gctgaacctg accaagcgtt gcaatctcaa atgtgatcac tgctatctcg atgcaaccac
      301 caaagccgcc ggtggagatg atgagctcag tactgaagaa tgctatcggc tcattgcgca
      361 gatcgcggaa gtgaataagg gctgtctcct tgtcatcacg ggcggcgaac cattcgtgcg
      421 gcccgacatc ctcgacattg ctcgttacgc ggtcaaactc ggctttatgg tcgtcttcgg
      481 gacaaacggg atgctgatca atgaccaact ggccaagact cttgtcgaga tcggtgtgat
      541 gggcgtgggc atcagcattg attctttgca ggccgccaag cacgacgcct ttcgcggcgt
      601 accgggggcc tgggaagcgg cggtcgccgg cattgaagcc agcaagcgga acggccttca
      661 attccaagtg catttcagcg ctcaaccgat gaactatacg gaactgcctg aggtcgtcgc
      721 ctgggcgcac cgacttggag cccgtgtatt gaatgtgttc tttatggttt gcaccggacg
      781 cggcgaagag ctgacggata tcacccccgc tcagtatgaa gaggtgcttg gatacctcat
      841 tgagtgccag gataattaca aaggcatgct ggtcagagcc cgttgcgctc cgcacttcaa
      901 acggttggct tatgagaagg atcctaactc tccgatcacc aaagccactg gatacatggg
      961 gggaggctgc ctcgcaggca ccaactacgc ccgggtgacg ccgaacggtg agttgactcc
     1021 ttgtccctat atgccgttgt cggcggggaa tgtccgccac cagagcttcg gcgatctctg
     1081 ggagcgatcg gatgtcttca attcattccg ctatcctcaa ctcaagggaa agtgcggcga
     1141 ctgtgaatat agcgacatct gcggcggttg ccgcgcccgt ccctatgttg accacggtga
     1201 ctggcttgat gaagatcagt ggtgcctcta caccccgaaa ggcggcgaga agatcaaagt
     1261 ggcgttcaat gtctccgaag agaccgacgt cacgtgggat gacgcatctg cactgagatt
     1321 gagccgtatt ccttatttct tacgcgccat ggtcaaaaaa ggcgtggaga aacacgcccg
     1381 ggaaaacgat gtgcggctca tcactgtcga attgatggag gagctgcgca aaaagcgatt
     1441 cggcaatgat gcaccggttt ttaaattcta gtgctgagca atgagtgctg agtcctgagt
     1501 ctgactgagt agagcgacgc cctggctggt tactcggcac tcgatactca tcacccaaca
     1561 ctaaccacga tggacactct tcagagtatt acgaccgatc tcaacatcct cattccgatc
     1621 atcaaccact ggtttcatct gctttcggcg gtcatttgga tcggcggatt ggcatttctc
     1681 gtcatggcgg tgacacccgg gctgcaaaaa gccgttccaa gggatcagat caaacccatt
     1741 acggacattt tttaccggta ctataagaag gtcgccggca ttctactggt cgtcctgctg
     1801 ttcaccggcg gcataaacct tcattatgtc aatcaagtct tggtttccca aagcggcaac
     1861 ggggtggaac accactccaa gtacctcatg gtcttcatga ttaaacttct tctcgttctg
     1921 ggcctcctca cactgtttct ttacaccgtt atctttcgat ccgaggacga aaccgacgaa
     1981 gaggaacctt atgaagccat ccccttccag cgggctgccc tctggatggg tttcttcatc
     2041 atcctctgtg cggcggccat gaagcatctg catcaatagt cctacgttgt gtctcgaacc
     2101 ggcttgaccg ttccaccgtc tcaaacgaga ctgtcactgt atcagttcga tacagtccct
     2161 tacattagac gattgtggat gcctttatat ccgatgacaa atcaactata tcagctcaca
     2221 tctatggtcc gtcatcctgg aataaatgtt gcttgttctc ctggcatgag tcacagatta
     2281 tcttctgaga taaatcatcc gcagcggacg tcagaccgga ctcaagagct gacttcaaat
     2341 acaaacgcac aagttgaacg gagaacggca cattccgtat tggttgtcga ggatgaccct
     2401 gatatcgcga tggcattgca agatatgctc gagttcgagg gattccatgt ggattgtgta
     2461 cagacctgtc gccgggcttt ctcatccatt gagcagaata tctacaatgc agttctcctc
     2521 gatctcggac taccggacgg agacggctgc tcgattttgg agaaactcca ggtctctcat
     2581 ccctcccttc ccgtgattgt tttaacggct tcgaataggg acctgtggcc tctacgtgcg
     2641 tatgctcgct tgagcaaacc gtggaagcga gaagagttat gcgggatact tcaccgtgct
     2701 atcggcacgg cgtcgtctta gacggtgagg tgaatcggca accaagccaa gaggcgactt
     2761 ttctcacttg aatcggctca atcccaaagc ccgcttgaag accgatttga agccgaagta
     2821 gccgaaggaa tctttgaggt tcgaatacaa tcgcttgacc gtccccatct ccggattggt
     2881 gacatactcc attgtgagtg caatccgctc ttcgccctcc ccgagaggcg tgacggcatg
     2941 ccagagcttg tccccgttga aaatcaccat atcgcctggt tcagtgatca actcaagatg
     3001 gcgcggctgc ttggtgggat ggtctttgaa aagctcgcac accaacttgc actgggttga
     3061 tcgatcaaca agccccatca agatggtgta ccgagcaccc ttatagtatg acgtatcgta
     3121 gtggaaccca atgtggtcgc caggttcagt gtagtagtag agagcgcagg aatggggatc
     3181 gttgtccgga cagtacacca gcttcgt
//
LOCUS       DATNDD010000284         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3774444, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000284 DATNDD010000000
VERSION     DATNDD010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774444"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VN647_06815"
     CDS             complement(<1..1163)
                     /locus_tag="VN647_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWG96779.1"
                     /translation="MDYPAPIGQARRLRSLLIMPVYQYQGYRNDGGAATGIIDAENVK
                     VARLKLRKEGVYPTDVVEQGQAPGRSREKATGRTERSVGRSATLSSTDLALMTRQFAT
                     LLVAGLPLVEALGVLVEQAEKKPIKALLADVREQIRGGKALSAVLGTYEKDFSPIYVH
                     MVRAGEASGALDQILFRLAEFLDKQLALRNKVTNAMLYPIIMLVIGSVILFFLITFVV
                     PKITLVFAQQKQALPWPTVALMSISQFFADYWMMLVGFVLGGFYMTRRFIRTGAGRMT
                     ADRLILKFPLIGDVARMVSISRLTSTLSTMLASGVQLLDAMDVSKRVMNNRVLEETVE
                     TARQNIREGETIADPLKRSGEFPALVTHMIAVGEKSGEMEEMLRRVSQIYDGEV"
     gene            complement(1207..>2847)
                     /gene="gspE"
                     /locus_tag="VN647_06820"
     CDS             complement(1207..>2847)
                     /gene="gspE"
                     /locus_tag="VN647_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HWG96780.1"
                     /translation="LAYQQEKGGLLGEVLLHLRLLREEQLLEALAQQFEMSWMPHLDT
                     THVDHELIKKVPISFCRRYRVLPLRYEEGAILTASTDPLETVALDDLRLLLGKPVKPV
                     LTTSVMLLACLNRAYDEIANPAGAEQVMEDIAANQSLDQLAHELDEPQDLLDATDEAP
                     IIRLVNSVLFQAVRQRASDIHFESFERGLVVRYRIDGVLYPVLTPPKHLQASIIARLK
                     IMSGLNIAEKRLPQDGRFGIRTAGKDVDLRVSVLPTSHGERVVLRLLEKENRLLNLSE
                     MGFPKERLAVIHQLIQLAHGIILVTGPTGSGKTTTLYAALSHINAPDKNIITVEDPVE
                     YQLLGIGQMQVNPKINLSFAAGLRSILRQDPDVIMIGEIRDRETAEIAIHASLTGHLV
                     FSTLHTNDAASAPTRLIDMGIEPFLVASSVVAVLAQRLLRRICPDCKRPYRASEEELS
                     RLDIPPGSDATLYRGTGCAACSQTGYRGRTGIFELMVLDDEIRRLIGSKADSTAIKQA
                     AIAKGMVTLKQEGAERVIQGHTTLEEVMRITQQEIEVE"
BASE COUNT          578 a          814 c          833 g          622 t
ORIGIN      
        1 acttcaccgt cgtatatttg actcactcgg cgcaacatct cctccatctc accgcttttt
       61 tcaccgacgg cgatcatatg ggtaaccagg gccggaaatt cgccgctccg ttttaacggg
      121 tcggcgatcg tctctccctc gcggatgttc tgtcgcgcgg tttcgaccgt ttcttcaaga
      181 accctattgt tcatgacgcg cttcgagaca tccatcgcat cgagcaattg cactccgctg
      241 gccaacatcg tggacagggt gctggtgagt ctggaaatcg acaccatccg tgcgacgtct
      301 ccgatcaatg gaaactttag tatcaaccgg tccgccgtca tgcgaccggc gccggtccgg
      361 atgaagcgcc gcgtcatata gaagcctccg agcacgaacc cgaccaacat catccaatag
      421 tcggcaaaga actggctgat cgacatcaac gccaccgtcg gccagggcaa cgcttgtttc
      481 tgttgagcga acacgagcgt aatcttggga acgacaaagg ttatgaggaa aaagaggatg
      541 accgatccga tcacgagcat gattatgggg tagagcatcg cattggtgac cttgtttctc
      601 agggcgagtt gtttatccaa gaactctgcg aggcggaaca agatctgatc cagcgctccg
      661 ctggcctcgc cggctcgtac catgtgaaca tagatggggg aaaagtcctt ttcgtacgtc
      721 cccaagacgg cactcaacgc ttttcccccg cggatttgct ctcggacatc ggcgaggagg
      781 gccttgatgg gttttttctc ggcttgttcc accaagacgc cgagggcttc gaccaacggg
      841 agcccggcca cgagcaatgt cgcaaactgt ctggtcatca gggccaggtc ggttgagctg
      901 agggtagccg atcggccgac ggatcgctcg gtgcggccgg tggctttttc ccgcgatcgg
      961 ccaggcgctt ggccctgctc gacgacatcg gtcggataga cgccttcttt tctcagtttg
     1021 aggcgagcga ctttgacgtt ctccgcgtcg atgatcccgg tcgcggctcc gccgtcgttc
     1081 cggtagccct ggtattgata gaccggcatg atcagcagac tccggaggcg ccgggcttgg
     1141 ccgatcggtg ccggatagtc caagatcgaa ccgactgaga ggagcggaca gcacgtcggt
     1201 gccgcatcat tcgacttcga tttcctgttg agtgatccgc atgacttctt ccagcgtagt
     1261 atggccttga atgactcgct cagccccttc ctgcttcaac gtcaccatgc ccttggcgat
     1321 cgccgcttgt ttgatggcgg tggagtcggc tttgcttccg atgagtcttc ggatctcatc
     1381 gtccagcacc atgagttcaa aaataccggt acggcctcga tagccggttt gggaacaggc
     1441 cgcacagccg gttcctcgat agagggtggc gtcggaaccg ggcgggatgt ccaagcgact
     1501 gagttcttcc tcgctcgcac gataggggcg cttgcagtcc ggacagatcc gccgcagcag
     1561 tcgttgggcg agaacggcga cgaccgatga ggccaccaga aaaggctcga ttcccatatc
     1621 gatcaggcga gtgggtgcac tggcggcatc gttggtatgc agggtggaaa acaccagatg
     1681 cccggtgagt gaggcatgaa tggcgatctc cgccgtttcc cggtcgcgaa tctcgccgat
     1741 cataatgaca tcggggtcct gccgaagaat cgagcgcagt ccggcggcga acgacaaatt
     1801 gatcttcgga ttcacttgca tttgcccgat gccgagcagt tggtattcca ccggatcttc
     1861 gaccgtgata atgttcttgt cgggggcatt gatatggctc aaggcggcat agagggtcgt
     1921 ggtcttgccg cttcccgtcg gcccggtgac gagaataatc ccgtgcgcga gctggatcaa
     1981 ttgatggatc acggcgagcc gttctttcgg aaagcccatt tccgaaagat tcaggaggcg
     2041 attttctttc tccagcaacc gcaacacgac cctctcgccg tgggaggtgg gcaagaccga
     2101 gacacgaagg tcgacgtctt tcccggcggt cctgatgccg aaccggccgt cttgcggcaa
     2161 gcgtttctcg gcaatgttga gaccggacat gatcttcaag cgcgcgataa tgctcgcttg
     2221 aaggtgctta ggcggcgtga ggaccgggta gagcacgccg tcgatacgat atcggaccac
     2281 cagtccacgc tcgaacgatt cgaaatggat gtcgctcgcc cgttgccgaa ccgcctgaaa
     2341 cagcaccgag ttgaccagtc gaatgatcgg agcctcgtcg gtggcatcca acaagtcttg
     2401 cggctcatcg agttcatgcg cgagctggtc gagactctgg ttggccgcga tgtcttccat
     2461 cacctgttcc gcgccggccg ggttcgcgat ttcgtcgtag gcccggttta aacaggcaag
     2521 cagcatgaca ctggtagtca agaccggttt gaccggcttg cccaacagta atcgaagatc
     2581 gtcaagcgcg acggtttcca gaggatcggt cgatgcggtg agaatggccc cttcctcgta
     2641 tcgaagcggc aacacacggt atcgtcggca aaacgagatg ggtacctttt tgatcagctc
     2701 gtggtccaca tgggtggtat cgagatgggg catccacgac atctcaaatt gttgagcgag
     2761 agcctccagc aattgctctt cccgcagtaa acgcaaatgc agcaggacct ctcccagcag
     2821 gccgcctttc tcctgctggt aggccaa
//
LOCUS       DATNDD010000285         4841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3495691, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000285 DATNDD010000000
VERSION     DATNDD010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495691"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VN647_06825"
     CDS             <1..267
                     /locus_tag="VN647_06825"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWG96781.1"
                     /translation="VGYNARPEGMGVLRVQVGAFADLQNARALFVQVQRDFPDGRIIQ
                     VDLPEGRRYRVQIGRFLTEPEAQVAMVRLDRIFSLQSFVVRDDS"
     gene            504..1769
                     /locus_tag="VN647_06830"
     CDS             504..1769
                     /locus_tag="VN647_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWG96782.1"
                     /translation="MDILILLGLIGLSAIISTAEIGFFSVNETRLKALAQNGSKRAEK
                     ALQLRSDPQKLFSTILVGDRLVSTAIPMFATFITLNAYGEKSIFEEAIAVMVGILTFV
                     LLVSVDVVPKTLAAKFSVPVTLNLAYPVYWVQVMLQPILFLIVPLIYSLTGGKGLTHP
                     LVTEEELKIMLDQGGKAGEIESEEVKMIKNVFQLKDITAEDAMTPRIYVFSLDGNLRL
                     KEAQELLYNSKYSRIPLYDGMLDNITGILYKTKALTELAKGRTDLRLRDIAHPPLFVP
                     AGKTADDLMKQFQQEKRHMGVVVNEFGGVMGLVTLEDLLEEVVGEIVDETDITEELIK
                     RIGKNQILVHGRTEVRKVNDFLKVELGDEALTVGGLIQENLGRIPQAGEELRIENCRL
                     VVHEADPRSIRSVQIFKDEKVPVPVEASV"
     gene            complement(1770..3830)
                     /gene="ligA"
                     /locus_tag="VN647_06835"
     CDS             complement(1770..3830)
                     /gene="ligA"
                     /locus_tag="VN647_06835"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWG96783.1"
                     /translation="MRQDSLYDDSLSSSRATRAEQERLTQLKSQIRHHDYLYYVKDRP
                     EISDGEYDRLFRELTDLERTYPELVTPDSPTQRVGASPLDELGKVQHERPMLSLDSLV
                     NPEEVLAFDQRMKRELGTDQVAYTVEPKFDGLSVEMVYDHGTFVRGSTRGDGNIGEDI
                     TINLRTIRSLPLQLRAGTYPDRLVARGEVYMRLSDFHALNRRMTERGDDAFANPRNAA
                     AGALRQLDSQITASRPLVVTCYEVMTVTGVLPASHWVELEALARWGLPVPTLRRRCET
                     IDQVLAFHRETENQRDNLPYEIDGVVVKVDRVDWQARLGMKSRSPRWAIAFKFPPRKE
                     ITEVQDIVISVGRTGTLTPLALLKPVEVGGVTISRATLHNADEVARKDIRIGDTVRVE
                     RAGDVIPAIAERIPVANEVRSDPFQMPDHCPICGSAVAREGAYFYCTGQAVCPAQLKG
                     AIEHFASKTALNIEGLGKKTVAQLVDQGLVKDLSDLYRLDRERLLELQGFAEKSATLL
                     FDAITRSKTAPLERFLMGLGIRQVGQHIAKVLAKEFCSLDAIMSADRDRLQEVREIGP
                     EISESLVSYFQESSNRRVIDQLLKLGFSIMSTPSPQSGAALPFSRKSFVFTGGLERLA
                     RDEAKALVERLGATVSSTVSKKTDYVVAGTDPGSKFEQAQKLGVTVLDEKTFLNLIEQ
                     QKEG"
     gene            4061..4705
                     /locus_tag="VN647_06840"
     CDS             4061..4705
                     /locus_tag="VN647_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWG96784.1"
                     /translation="MQKGEQQVYERTRRIPLLGILLCSGLLVSGCVVLEEKYNAEKAR
                     SLNFQRLLAQEEKRTAELDSEIRHTKAELAEFEVRNRELSAQVQVAREQMGRLQEETE
                     AIREATVLERKALEDMQRKGPSLSSKPKKVEASKAVSGGRSGGSLSDKGMAAVTEPAS
                     AMKVTPGMVHVVKPGETLFSIGRRYGVEVDKLKKLNKLPDDIVEIGQKLLVGAE"
BASE COUNT         1137 a         1225 c         1360 g         1119 t
ORIGIN      
        1 gtcggttata acgctcgacc cgaagggatg ggagtgttac gggttcaagt cggcgccttt
       61 gcggatcttc aaaacgcccg ggcgctgttc gtgcaagttc aacgggattt tcccgatggg
      121 cgtattattc aggtggatct accggaaggg cgacgttacc gagtccaaat cggtcgattc
      181 ttgaccgagc cggaagctca ggtggctatg gttcgcctcg atcgtatatt cagcctgcaa
      241 tcatttgttg ttcgcgatga tagttgaaca ctatcggcgt ggatcgtttg gaaccagttg
      301 attttaaagc aacattttca gaattacttg cttgcgtttg atctctgtga tagatgtatc
      361 agctgtgaac tctattctaa gcggtccggt tatcctaatc ttacgatcct ctgaatggtg
      421 ggccaagagg tatcctctgg gagacgcccg gcttctcatc aggccagcca cactggtcca
      481 gcgtccctaa atatacgact gtgatggaca tcctcatcct tttagggttg atagggttat
      541 ccgctattat ttctacagct gagatcggct tcttctcggt taacgaaaca cgactgaaag
      601 cattggcgca gaacggaagc aaacgagctg aaaaggccct tcaactaaga agcgaccctc
      661 agaagctctt ttcgaccatt cttgtgggag atcgtctggt cagtacggca atcccgatgt
      721 tcgccacctt catcacgttg aatgcctatg gggagaagag tattttcgag gaagccatag
      781 cagtcatggt cggtattctg accttcgttc tcttagtatc cgtggacgtg gtcccaaaga
      841 ctttggccgc taaattttct gtgcctgtta ctctgaacct tgcatacccg gtctactggg
      901 ttcaggtcat gttgcagccg atccttttcc tgattgtccc tctgatctac agtttgacgg
      961 gtggaaaggg actgactcat cccttggtga cggaggaaga gctgaagatc atgctggacc
     1021 aaggaggaaa agccggcgag atcgaatctg aagaagtgaa gatgataaag aatgtattcc
     1081 agttgaagga tattacggcg gaagatgcga tgacgccgcg catctatgtg ttttctctcg
     1141 acgggaacct tcggcttaaa gaagcacaag aactgctcta taattctaag tattccagaa
     1201 ttccgctcta tgacgggatg ctcgataaca ttacggggat tctctacaag acgaaagcgc
     1261 tgaccgagtt ggccaaaggg agaactgatt tacgccttcg ggatatcgcc catcctccgc
     1321 tcttcgtccc ggccggcaaa acagcggatg atttgatgaa gcagttccag caggaaaagc
     1381 gacacatggg agtcgtcgtc aatgaattcg gtggtgtcat gggattggtg acgcttgaag
     1441 accttctcga ggaagtagta ggtgagatcg tcgatgaaac ggatatcacc gaagaactca
     1501 tcaaacgcat cggaaagaac caaatcctgg tccacggacg gacggaagtt cgaaaggtga
     1561 atgacttcct gaaagtcgag ctaggagatg aagcgctgac tgtcggaggg ctgatccagg
     1621 aaaatcttgg tcgcatcccc caggcgggag aagaactccg tatcgagaat tgccgcctcg
     1681 tggtccatga agcagatcct cgctcgatac ggagcgtgca aattttcaag gacgagaaag
     1741 tgcctgttcc ggtcgaagcc tcggtgtgac tagccttctt tttgttgttc tatcaagttt
     1801 aggaaagtct tttcgtcaag gaccgtcacc cctaatttct gagcctgttc gaattttgag
     1861 ccgggatctg tgccggctac gacatagtct gtcttcttgc tgaccgtgga tgataccgtg
     1921 gcgcctaacc gttcgaccaa tgccttggcc tcatccctcg ccagcctctc taatccaccg
     1981 gtaaacacga agctcttcct tgagaagggc aaagctgccc cgctctgagg agagggtgtg
     2041 ctcatgatcg aaaaccccaa tttaagcagt tggtcgatga cccgtcggtt ggaactttct
     2101 tggaagtagg agaccaaact ctcggatatt tcaggaccaa tctccctgac ttcttgcaaa
     2161 cggtcgcggt cggccgacat gatggcatcg agagaacaaa actctttcgc gagcacttta
     2221 gcgatgtgct gtccaacttg acggattccc aggcccatca aaaatcgttc gagcggtgcc
     2281 gtcttacttc gagtgatggc gtcaaaaagg agagttgcag atttttccgc gaacccttgg
     2341 agctccaaga gtcgctcccg gtcaaggcga taaagatcag aaagatcttt gacgagtcct
     2401 tgatcgacta attgagcgac tgttttcttc ccaaggcctt caatgttcag ggcagttttc
     2461 gacgcgaagt gctcgatcgc gcctttcagt tgagcaggac agacggcttg gcctgtgcaa
     2521 tagaagtagg cgccttctct cgccacggcg gaaccgcata tcggacagtg atccggcatc
     2581 tggaaaggat ccgacctgac ttcatttgcg actgggatac gctcagcaat cgccggaatg
     2641 acatcgcctg cgcgctcgac tcgaaccgta tcgcctatgc ggatgtcctt tcttgccacc
     2701 tcatcggcat tatggagggt tgcgcggctg atggtgaccc cacccacctc caccggcttc
     2761 agcagggcga gaggggttaa ggtccccgtt cggccgactg aaatgacaat gtcctgtacc
     2821 tccgtaattt cctttcgagg aggaaacttg aatgcgatgg cccaacgagg acttctggat
     2881 ttcattccca gccgcgcttg ccaatccacg cgatcgactt tcaccacgac tccatcaatt
     2941 tcataaggga gattatcgcg ctggttctcc gtttcccggt gaaatgccaa gacctggtct
     3001 atggtctcgc atcgtcggcg aagggtaggg accggaagcc cccaccgggc cagtgcctcc
     3061 agttcaaccc agtgcgacgc gggaagaact ccggtcaccg tcatcacttc atagcaagtg
     3121 acgacaagcg gccgagaggc ggtaatctgc gaatcgagtt gccgaagagc gccggcggcg
     3181 gcattgcggg ggtttgcaaa ggcgtcgtcc ccgcgttccg tcattcggcg gttgagcgca
     3241 tgaaagtcgg agaggcgcat atatacctct ccccgcgcga ctaggcggtc cgggtaggtg
     3301 ccggcccgca actggagcgg taacgatcga atcgtgcgga gattaatggt gatatcctct
     3361 ccgatattgc catcaccacg ggttgaaccg cgaacaaacg tcccatggtc gtagaccatt
     3421 tcaactgata gaccgtcgaa ttttggctcg actgtgtacg caacttggtc ggtgcccagt
     3481 tcccgtttca tccgctggtc gaaggccagg acttcctcag gattcaccaa cgaatcaagg
     3541 ctcaacatag gccgttcgtg ctgtaccttg ccgagctcgt ccaaagggga ggcaccgacg
     3601 cgctgggtcg gagaatcagg ggtgaccagt tccgggtacg tccgttcgag atcggtcaat
     3661 tctcggaata accgatcata ctccccatcg gaaatctcgg ggcggtcctt gacgtaatac
     3721 aggtagtcat gatgcctgat ttggcttttg agttgagtca gtcgctcttg ctcggcgcgt
     3781 gtggctctcg aagaggacag agagtcgtca tagagtgaat cctgtcgcat cgctgctctc
     3841 aacgagatca aatctcgacg atcatctcta aggtgccacg acggatgacg ttcggcatga
     3901 aaggagagag gcctgaaccc cgctgaccct agcatagggc cgcggaaacg gtcaaaccgg
     3961 acaggcccgg tccgctttga ctttcgcatc agtgaccact attctaagcg tctcttcggc
     4021 aggtctgaag tcgccggaaa gcctgggcaa aggaggatgt atgcagaagg gagagcagca
     4081 ggtctatgag cgcacacgga ggattcctct cttagggata ctgctctgta gtggtttact
     4141 ggtcagcggg tgtgtcgtgt tagaggaaaa atacaacgcg gaaaaagccc gaagtctgaa
     4201 ttttcagcgc cttctggctc aggaagagaa gcgaacggct gagctcgaca gcgaaatcag
     4261 gcacaccaag gctgaactgg ctgaatttga ggtgagaaat cgcgagctgt ccgcacaggt
     4321 gcaggtagca cgagagcaga tggggcgcct ccaagaagag acggaagcga tccgggaagc
     4381 gaccgtgttg gaacgaaaag ccttggagga tatgcagcgg aaaggccctt ctctttcctc
     4441 taaaccgaaa aaagtcgagg cgtcgaaagc tgtttccggc ggtcgcagcg gaggcagtct
     4501 ttccgacaaa ggtatggcag ctgtgaccga gccggcttct gccatgaagg taacgccggg
     4561 gatggtgcat gtggtgaagc cgggggagac tctcttcagc atcgggagac ggtatggagt
     4621 cgaggtcgat aagttgaaga agctgaataa gctgccggat gacatcgtcg agatcggaca
     4681 gaagctcctg gtgggagccg agtaagcaga agacatgaag gccgcgacct attcactcac
     4741 tctggatgaa tttcgctcgt acgcaaaaca gggcaatctc attccgttgt tccgtgaaat
     4801 cttggcggac catgatacgc cggtctcggc ctttgccaag a
//
LOCUS       DATNDD010000286         4465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1996988, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000286 DATNDD010000000
VERSION     DATNDD010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996988"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VN647_06845"
     CDS             <1..297
                     /locus_tag="VN647_06845"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG96785.1"
                     /translation="VQVDYPALFTGDEGSGHGTVTNLTIAGCEIESHVQFPIGTGLSL
                     RVQAPEARPPIIIALAIVRWKNGDRFGLEFVRFEGRTKEQLGDMLNQREGPAEG"
     gene            450..980
                     /locus_tag="VN647_06850"
     CDS             450..980
                     /locus_tag="VN647_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWG96786.1"
                     /translation="MRRLILAFGLLVFPMLCTTPVSTAGLFQVGEKAPSFTLTAITGE
                     TVSLESYKGKVVVLGLFHICDPCLIQGSTLQKVHESTQGKNVAVLGVNSSGNAKREVG
                     EFLSGFPVKVTYPYLLDPTKVTDKLYGGGKFIPNVYVIDQNGVIRWQRVGNMDLAGAD
                     VIVAEVEKLLVGGNKM"
     gene            1137..1376
                     /locus_tag="VN647_06855"
     CDS             1137..1376
                     /locus_tag="VN647_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96787.1"
                     /translation="MDEMEEGKQKFLEVVQGIDRSVQVVIPATPSNSMFLISLTKGSN
                     RKFITVPEDDIIDLPHEASIQTKVTKTIKDAIAAL"
     gene            1430..2032
                     /locus_tag="VN647_06860"
     CDS             1430..2032
                     /locus_tag="VN647_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96788.1"
                     /translation="MKQILPDIWQWSWFSDEKQLDFNGLFLMIGEHKILVDPPPMTAE
                     ARAVVRRQEPIDYILITNRDHLREAVVYQIEFKCQLRVPEADAAQMDVKPTATYKDGE
                     LLPGGIWAIHLKDQKSPGESALFIERGRGVLIVGDALIGKPPGSARLLPPEKYVDVQK
                     AKDGLCRLLKYNFDSLLVGDGASILVGAKQQVAQLLSVTS"
     gene            2029..2853
                     /locus_tag="VN647_06865"
     CDS             2029..2853
                     /locus_tag="VN647_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96789.1"
                     /translation="MTLRNSLSEELNRQGNEHFSRGYYTEAYICYAKALGYDRLTGDQ
                     RALVATLGNLGNICAVSGRRDSAQAHYQEVLELQKLLGDEKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDLMTKTHDEPAQAVLFSDLGLVARETGHFDEAIQFYERSLELMRR
                     LGNLGGMADAWRMIGRTFLIQKRYDEAIACCQTSQSVAERLRDELRAGGARYMLAQCY
                     EDMGRLQDAADLLEQVVHMDRKYRLPKLEENMQRLETLRARLADSYQSRSCRGVQA"
     gene            2850..3287
                     /locus_tag="VN647_06870"
     CDS             2850..3287
                     /locus_tag="VN647_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96790.1"
                     /translation="MTEPSSSAWSQLRADLMRSFPQFYELQSNGPLLMDLGGDGWLLE
                     VRPDGKVLCQYGVAMDEVIALMSEGTPEDLGTDEAAKQAKYFLQPAVSKYRALLLQSG
                     FVEETEMTDEFVAITFTRGADLQNLTKLEDLLRWCYRQIGSAS"
     gene            3284..4339
                     /locus_tag="VN647_06875"
     CDS             3284..4339
                     /locus_tag="VN647_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWG96791.1"
                     /translation="MTQRITTFDEFRDAVSAYRLPRVLLVALELDLFTTVGDRSWTIS
                     DLAKELKVSERGLSILCRNLAAVGVLQKKGANYRNSRLGATALNADHRAYRGGYLNLI
                     KRHWTDWIRLSESVRSGLPIGHDVPDSPDYRRQFTWAMHHRTLEIAPAIAAQVHMGRT
                     KTLLDLGGGPGTYAMAFLAKNPKLHATVCDREVALEVSKEIAGAHKARRRLSYLPLDF
                     SNEPIAGTYDVIWYSNVLHMYSPEENRAIFRRALAALTPGGRFIIQDAFLHDREGLYP
                     LEASLFAVSMLLFTEGGNTYSASETAKWLKGAGFVSVKSLPIRKGTEDWEDGVLEASA
                     PGLHPKATARRTQSRRN"
BASE COUNT         1050 a         1094 c         1287 g         1034 t
ORIGIN      
        1 gtgcaggtcg attaccccgc attattcacg ggagatgaag gatcaggcca tggaacggtg
       61 accaatctca cgattgccgg atgtgaaatc gagagccatg tccagttccc catcggaacc
      121 ggtctgagtc tccgtgtaca agctccggag gctcgacctc cgattatcat cgcccttgcc
      181 atcgtccgtt ggaagaatgg tgatcgattt ggactcgagt tcgttcgatt tgagggtaga
      241 accaaggaac agctcgggga tatgctgaat cagcgtgaag gccctgccga aggttaattt
      301 ctcctgcctt gcggttgccg ctccaaaaat tgccatggta ggatgtatca gcagctgatt
      361 ttgcgcagcg gtaacggctg taccacgacg actggatcat ggtcgagcgc ttgtgaggcc
      421 atgagtgttc gtttgaaagg gaggtcgtga tgagacgact tatactggcg tttgggctgc
      481 tggtcttccc catgttgtgt acgacaccgg tttcaacagc gggattattt caagtgggag
      541 agaaagcccc atccttcacc cttaccgcga ttaccggtga aaccgtgtca ctggagtcct
      601 ataaaggtaa agttgtggtg ctggggctct ttcacatctg tgatccttgc ctaatacagg
      661 gaagcacctt gcagaaggtc catgagtcta cgcaaggcaa aaatgtagca gtgctgggtg
      721 tcaattcgtc aggcaacgcg aaacgagagg tcggcgaatt cttgtctggc tttccggtca
      781 aggttaccta tccctatctt ttggacccca ccaaggtgac ggataagctg tacgggggag
      841 gaaagttcat tccaaatgtg tatgtgatcg accagaatgg agtcatccgc tggcaacgag
      901 tcggcaatat ggacttggcg ggcgccgacg tgattgttgc agaggttgaa aagctgttag
      961 taggcggaaa taagatgtag gggacgggga cggatgtttc tctgtgctcg ctgaccaccc
     1021 gccaagactc catcgaatgt agaaagttgg cggcggtcgg tcaatgcgcg cagtgagaga
     1081 aacatccgac cccgcccgcg cgtaaggaga aagagtgtag agaggagaag gaagtgatgg
     1141 atgaaatgga ggaggggaaa caaaagtttc tagaagtggt gcagggaatc gacaggtctg
     1201 tccaagtcgt gattcctgcc actccctcca acagtatgtt cttgatctcg ctgacgaaag
     1261 ggtcgaatcg taaatttatc actgtgcccg aagacgatat tatcgacttg cctcacgaag
     1321 cgagcatcca gacgaaagtc accaagacga tcaaggacgc catcgcagct ctgtgatgcc
     1381 tgtggtgtca taatccccaa ctataaatat aagccgctgg gtttttgtta tgaaacaaat
     1441 cttgcccgat atctggcaat ggtcgtggtt ttccgatgag aagcagctcg acttcaacgg
     1501 attgttcctg atgatcggtg aacataagat cctagtagat ccacctccca tgacggccga
     1561 ggcgagagcg gtggtccgtc gccaagagcc gattgactac atccttatca ccaaccgaga
     1621 tcacctccga gaggctgtgg tttatcagat agagttcaag tgtcagttgc gagtgccgga
     1681 agctgatgct gctcagatgg atgtgaaacc aacagcgacc tacaaggatg gtgagctatt
     1741 acctggtggg atttgggcta tccatctgaa agatcaaaag tctccgggag agtccgcgtt
     1801 gtttatcgaa cggggtcgag gtgtgctgat cgtaggggat gcgttgatcg gcaaaccacc
     1861 cggttccgcg cgtctgcttc ctccagaaaa gtacgtggat gttcagaaag ccaaggatgg
     1921 tctctgccgc ctcttgaagt acaacttcga tagccttctt gtcggagatg gggcatccat
     1981 ccttgtcggt gcgaagcagc aagtggcaca gctcttgtca gtgacgtcat gacattacgt
     2041 aacagcctat ccgaagaatt gaatcgacaa ggcaacgaac atttctcacg agggtactat
     2101 acggaagcct atatctgtta tgcgaaggcg ctagggtatg atcggctcac cggtgatcaa
     2161 cgtgccctgg tcgccactct tggtaacctg ggtaacatct gcgcagtcag tggacggagg
     2221 gattctgccc aggcgcatta tcaggaagtt ttggaattgc aaaaactgct cggtgatgag
     2281 aaaggtatcg ggacgacgct ggcaaacctg ggaaacctcc gggctgatgc cggtgaatgg
     2341 gatagggcac gagcctatta tctggaagct ctcgacctca tgaccaaaac gcatgatgag
     2401 ccggcccaag ccgttctgtt ctcggacctt ggtctcgtag ctcgtgaaac cggtcacttt
     2461 gacgaggcga ttcagtttta cgaacggtcg ttggagttga tgcgtcggtt gggcaatttg
     2521 ggcggtatgg cggacgcttg gcgtatgata ggtcgcacgt tcttgataca aaagcgctac
     2581 gatgaagcca ttgcctgttg tcagaccagc caatcggtcg ccgagcgatt acgagacgag
     2641 cttcgtgccg gtggcgcccg atacatgctg gctcaatgtt atgaggatat ggggcgtttg
     2701 caggacgcag cagacctcct cgaacaggtg gtgcacatgg atcgtaaata tcgcttgccg
     2761 aaactggaag aaaacatgca gcgcctagaa accctgcgcg cccgcctcgc cgattcgtat
     2821 cagtcgcggt cttgtcgagg ggtgcaggca tgacggagcc ctcttcctca gcatggtcac
     2881 agcttcgtgc tgacctcatg cggtctttcc cacagtttta tgaattgcag tccaacggcc
     2941 ctctcctgat ggatcttggt ggagatggtt ggcttttgga ggtcaggccg gacgggaagg
     3001 ttctttgcca gtacggggtg gcgatggatg aggttattgc gctcatgtcg gaaggaacgc
     3061 cggaggattt agggaccgat gaagccgcga agcaggccaa gtacttcctt caacccgctg
     3121 tttccaaata tcgagcgctc cttcttcagt ccgggtttgt tgaggagacc gaaatgactg
     3181 atgaatttgt ggcgatcacg tttacacgcg gtgcggatct gcagaatctc accaagctgg
     3241 aagatctcct ccgatggtgc tataggcaaa tcggaagcgc gtcatgaccc aacgaatcac
     3301 cacattcgac gaatttcggg acgctgtctc agcctatcga ttgccacgag tgttgctggt
     3361 cgcgctggag cttgatcttt ttacgactgt cggcgaccga tcgtggacga tctccgatct
     3421 tgccaaggag ctcaaggtca gcgaaagggg tttgagcatt ctttgtcgta atctcgcagc
     3481 cgtcggggta ttgcagaaaa aaggagccaa ctatcggaat agccggctag gagcaacagc
     3541 gctgaatgcc gaccatcgcg catatcgcgg aggctatttg aatctgatca aacgccattg
     3601 gacggactgg atacgactgt cggaatccgt gcggagcggc ttgccgatcg gccatgatgt
     3661 tcccgacagt ccggactacc ggagacagtt cacgtgggcc atgcaccaca ggaccttgga
     3721 aattgccccg gcgatcgcgg cgcaagttca catgggtcgt acgaaaacac tgctggatct
     3781 gggaggaggg ccaggcacct acgccatggc gtttctcgcg aagaacccca agcttcacgc
     3841 caccgtctgt gatcgagagg tcgctcttga ggtctccaaa gaaatcgccg gcgctcataa
     3901 ggcgaggcga cgactctctt atcttccgct cgatttctcc aacgaaccta ttgcaggaac
     3961 gtatgatgtg atctggtatt cgaatgtgtt gcacatgtat tcacccgaag agaaccgagc
     4021 catctttcgt cgagccttgg ccgcattaac acccggtggt cgattcatca ttcaagacgc
     4081 ctttctccac gatcgcgaag gtttgtatcc gttggaagca agtctgtttg ctgtgtcgat
     4141 gttattgttt acggaaggcg gaaacaccta ttcagcctca gagacggcca agtggctgaa
     4201 aggtgccgga ttcgtctcgg tcaaatcgct tcccattagg aaggggacgg aggattggga
     4261 ggacggggtt ctagaggcat cggcaccggg cctacatcca aaagcgaccg cccgccgaac
     4321 acaatcaaga agaaattgaa gagcccgctg attacgttag ctatcaagga cactcccact
     4381 tctgaatctt ttgtcgtcac ctgaatgatt gtcgctatgt ccagtgccaa tccaaccgtg
     4441 gcgtagatta cgcaagccat ggcgg
//
LOCUS       DATNDD010000287         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2871634, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000287 DATNDD010000000
VERSION     DATNDD010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871634"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..913
                     /gene="ureG"
                     /locus_tag="VN647_06880"
     CDS             242..913
                     /gene="ureG"
                     /locus_tag="VN647_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWG96792.1"
                     /translation="MHDINKEHRHDWVPTQARVKGIPVIGIGGPVGSGKTALVEALCQ
                     RLRDRYSLAAVTNDIFTKIDAEILTKRAALPVDRIVGVETGGCPHTAIREDASHNQEA
                     IDDLLRRHPDVELIFLESGGDNLAATFSPELVDSVIYVIDVSGGDKIPRKGGPGITRS
                     DFLVINKMDLAPYVRADLSVMDRDTRRMRGDLPFAFTNILSGEGLDAVVAWMEQRIPQ
                     RARRS"
     gene            916..1875
                     /locus_tag="VN647_06885"
     CDS             916..1875
                     /locus_tag="VN647_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWG96793.1"
                     /translation="MQASTRPRKKTCSRRRTAQKRNTSEKRSPSRKRSTSPTFSTEFI
                     GRVGELRLDYAKPDRRTIIAHSYFTTPWKLLPPIYLDDTGAAYTLLVNPSGGLVGGDH
                     LSIDMSLDRDAHVLISSPSANRVYRTESKISQQHINITVGPGAILEWFPEHTIPFAGS
                     RFRQILHATLAPGATLLLWDAVASGRIARGERWAFTDLENEIRITTASGSSLLERYVL
                     DPTTDLGCVGLAEEWNYVASFYVVNDVVASDTWSRLESRIASILDERPGHVLGGVSTP
                     TVPGVAIKLLTQTAPDLTYMLDTLWATIREELWNLPPVSLRKY"
     gene            complement(1951..>2376)
                     /locus_tag="VN647_06890"
     CDS             complement(1951..>2376)
                     /locus_tag="VN647_06890"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWG96794.1"
                     /translation="PVVMWQHRYYTPIFLSGLVLPFVVGFLYAGWIGGASCFMLAGVG
                     RTFAVLNSTFCINSVCHLWGSQPHSQADSSRDSWLVSLLTFGEGYHNYHHTHQSDYRN
                     GPLWYNFDPSKWMIFMLSLLGLAWSLRTANAADSRSSNL"
BASE COUNT          564 a          637 c          663 g          512 t
ORIGIN      
        1 cagcatgaag gacaacgcgt acttagtgaa tggcttccac tgatcgatcg catcagtcgt
       61 gaaaccgact cggcaacaac gatgagctcg tggtctccta tccaagatat ctattcgatg
      121 cgccatggtc ggttggagtg gaggctcttc cggtcttgag aattgacaac tatcattcat
      181 aacagaaagg gaagagaagg ctgatgccgg ggcgataggc cctgaaccct tcgagtttgc
      241 catgcatgac atcaacaagg aacatcgcca cgactgggta cccactcagg caagagtgaa
      301 aggcattccg gtcatcggaa tcggggggcc ggtgggttcg ggcaagaccg cactcgttga
      361 ggccctgtgt cagagattgc gggatcggta cagtttggca gctgtgacaa acgacatttt
      421 tacaaaaatt gatgcggaaa ttctgacgaa gcgcgcagcc ttgccggtcg atcggattgt
      481 gggcgtggag acgggtgggt gccctcacac ggccatccga gaagacgcct cgcataatca
      541 agaagcgatc gatgacctgc ttcggcgcca tcctgatgtt gaattgatct ttctggaaag
      601 cggaggagac aatctggcgg cgaccttcag cccggaactt gtggattctg tcatctacgt
      661 gatcgatgta tcgggaggcg acaaaattcc acgcaaaggc ggtcccggca ttactcgatc
      721 ggattttctc gtcatcaaca agatggattt ggcgccgtac gtccgcgccg atctgtcagt
      781 catggatcga gatacccgcc ggatgcgcgg cgatcttccc ttcgcgttca ccaacatcct
      841 ttccggtgaa gggcttgatg ccgtggtggc ctggatggag cagcgcatac cgcagcgagc
      901 gaggcgctca taatcgtgca ggcttcaaca agaccacgaa aaaagacttg ttcgcgaaga
      961 cgtacggctc agaaacgaaa cacttctgaa aaaagaagtc cctccagaaa aagaagtacg
     1021 tcgcccacgt tttccaccga atttatcgga cgggtcggtg agcttcggct tgactatgcc
     1081 aagcccgacc gccgaaccat catcgcacac tcgtacttca cgacgccatg gaagctcctt
     1141 ccgcccattt atcttgatga tacgggggca gcgtacacgc tgctcgtcaa tccatccgga
     1201 ggcctggtcg gaggggacca tttgtctata gacatgagcc tggatcgaga cgcgcacgtc
     1261 ctgatctcct caccgtccgc caatcgggtc tatcgaaccg agagtaaaat ctcccaacag
     1321 cacatcaata ttaccgttgg gcctggtgcg atcttggaat ggttccctga acacaccatt
     1381 ccgttcgccg ggtcacgatt tcggcagata cttcatgcta cactggcgcc tggtgcgact
     1441 cttctcttgt gggatgccgt cgcatccggg cgtatcgcca ggggggagcg atgggcgttt
     1501 accgacctgg agaacgaaat ccgtatcaca acggcatccg gtagttctct tctggagcga
     1561 tatgtccttg atccgactac tgatttgggt tgcgtagggc ttgcagaaga atggaactat
     1621 gtcgcctcct tctatgtggt caacgatgtc gtcgcgtccg atacctggag ccgactggag
     1681 tcgaggatcg cgtctatcct ggatgaacgg ccgggccacg tgttgggagg agtatcgacg
     1741 ccgactgtgc cgggggtcgc catcaaattg ctgactcaaa cggcacccga cctcacctac
     1801 atgcttgata ctctctgggc aactatccgt gaagagctgt ggaatcttcc acctgtatcc
     1861 ttgcgaaaat attaacaggc tgttgaaaaa gtccgccagc gtcgttctcg cccgctgcgg
     1921 ggacatcgac tgctccgcgc tagagatcgc ttagagattt gaagatctgg aatcggccgc
     1981 attggcggta cggagcgacc aggctaatcc cagaagcgaa agcatgaaaa tcatccattt
     2041 tgatggatcg aagttatacc agagaggacc gttgcggtag tcgctttgat gggtgtgatg
     2101 atagttgtga tacccctcgc caaaggtcag gagcgagacc agccagctat ctcgactgga
     2161 atccgcttgg ctgtgtggtt ggcttcccca gagatgacaa accgaattga tgcaaaaggt
     2221 cgaattcagc acggcaaagg tccgacccac tcctgccagc ataaagcagc tggcaccgcc
     2281 gatccacccg gcatagagaa agcccactac gaacgggagc accaaccctg acagaaagat
     2341 cggcgtgtag taacgatgtt gccacatcac aactgg
//
LOCUS       DATNDD010000288         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2764367, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000288 DATNDD010000000
VERSION     DATNDD010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764367"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="mscL"
                     /locus_tag="VN647_06895"
     CDS             complement(<1..305)
                     /gene="mscL"
                     /locus_tag="VN647_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWG96795.1"
                     /translation="MLKEFKEFAMKGNVLDMAIGVIIGGAFGKIVSSLVGDVLMPPLG
                     LLMGKVDFSSLFINLSETSHPSLVAAKAAGAPTLNYGVFLQSVFDFLIIAFVIFLLV"
     gene            complement(352..1449)
                     /locus_tag="VN647_06900"
     CDS             complement(352..1449)
                     /locus_tag="VN647_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HWG96796.1"
                     /translation="MKRSVILVAILGLITMAHPAFADEGPDPMTPAAALDVVTLTDGS
                     VIYGEVVEMTSGLLQIKSSMADDLIKVKWAEVGKLKVTHPIPFHLKEDIVLVGTVEEG
                     DPGMMKLKAEPTGSMMTVPMDSVTQVNPLVQPQIIYTGNMNASYSQATGNSHLRSISL
                     VGDLVARSEQRRLTILGRYVYGDDAGSLITRNARGTIKLDHFITKRFFWYESVYLEND
                     RFQGIKLRTSISSGPGYQWIDRGDLTGIFKDMTLYSEAGPAYFNEDHINPLPDTISFR
                     GRVAMKWDWPLFDGKIVLHHDDEMFPSLQNFSEFYFTMNNWVHFTILNGLTSGFQVTT
                     RYDNRPLAGKIKTDNLYLLTLGYAFDTTRKR"
     gene            complement(1593..2399)
                     /locus_tag="VN647_06905"
     CDS             complement(1593..2399)
                     /locus_tag="VN647_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWG96797.1"
                     /translation="MTVVDTLTHYAVQYGLQAAVAFGILVAGSMASRWAGNFAQQTLE
                     RQTLEPPVRLLLVRIVKIVVMLFTAMIALQTLGVPIAPLIAGLGVAGVGIGLALQGVL
                     SNVMAGLSIIFSKPYKVGEHISLLSVHGDVVMIDIFTTTLMHADRSRVIIPNRKIVGE
                     ILHNFGTIRQLHLTIPVSPNANLDEAIVQVRDVLDRHPSVMKDPVPSVGVSSLGESSL
                     MVAIAVEPWTAAADYSSTQGELNKLILQRFQERGITLPSSSLTVRMVNDR"
     gene            complement(2482..>3546)
                     /locus_tag="VN647_06910"
     CDS             complement(2482..>3546)
                     /locus_tag="VN647_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWG96798.1"
                     /translation="RTPRLHFLSLVQPFNLPVKLDGEFGPLRETMDIEAINFELGMGK
                     TDFTITGNAAGNDANVYITSPVITTANLPVSLPLKKPVDIKNFQIAAEVKGQGIKLNT
                     LSFQLFDGQMKGQGKLIAGPDAPPFKGGVTVQGVQLGPALDAVAEAPISVSGTAGADL
                     SLQGIGFSMPDLTTALEGTGHMTVKDGKIEGVNLLQEVVSALKVAGISLDNAKATAFS
                     TIETDLAIKQGIINVQHLLMDSHDFQATGGGTIGFDQRLNLLVNLNLSQDLSRKIAAA
                     SPVVKLAVKDGRLSLPLTITGTAQAPTYGVDLKGVTGKVHQQVRKKVEEAVDGLIKGT
                     KTPEDLKKEGQELLKGLFGR"
BASE COUNT          824 a          984 c          916 g          822 t
ORIGIN      
        1 ttgaccaaga gaaaaatcac aaacgcaata ataagaaaat cgaatacact ttgcaggaag
       61 accccgtaat tgagcgtcgg agcccctgct gccttcgctg ccactaagga cggatgggat
      121 gtctcggaca ggttgatgaa caagctggaa aaatccacct tgcccatcag caacccaaga
      181 ggcggcatca ggacatcacc gaccagcgac gaaacgatct tgccgaacgc tcctccgatg
      241 atgacaccga tcgccatatc caggacattc cccttcatgg cgaattcttt gaattccttc
      301 agcatagtgc acctcctaaa gagcgcggtt cagtacgatc ccacaagcga tctaccgttt
      361 acgggtggtg tcgaacgcat aacccagcgt gagcaagtac agattgtctg ttttgatctt
      421 gccggcgagc ggccgattgt cgtagcgcgt ggtcacctga aacccgctcg tcagaccatt
      481 caagatcgtg aagtgcaccc agttattcat cgtgaaataa aactcagaga aattttggag
      541 ggacgggaac atttcgtcat catggtgcag cacgatcttg ccatcgaaca acggccaatc
      601 ccatttcatg gctacccgtc cgcgaaaact gatcgtatcc gggagagggt taatatgatc
      661 ttcattgaaa taagccggac cggcttcact gtaaagtgtc atgtctttga aaatgcccgt
      721 gaggtctccg cgatcgatcc attggtaccc tggtccgctg gagatcgagg ttcgcagttt
      781 tataccttga aatcgatcgt tttcgagata cactgattca taccagaaga accgtttggt
      841 gataaaatgg tccaacttaa tggttcctcg agcattgcga gtgatgaggc tgccggcatc
      901 gtcgccatag acgtaccggc ccagtatggt caaccgacgt tgttcacttc gagccacaag
      961 gtctcccacc aggctgatac tccgaagatg actgtttcct gttgcttgag agtagctggc
     1021 attcatatta ccggtgtaga tgatttgtgg ttggaccaac gggttcactt gagtcaccga
     1081 atccatcggc acggtcatca tgcttccggt cggctctgct ttcagtttca tcattccagg
     1141 gtcgccctcc tcgacagtcc cgaccagaac aatgtcctcc tttaaatgaa agggaatggg
     1201 atgagtgacc ttcagcttgc cgacctcagc ccatttgact ttgatgaggt cgtccgccat
     1261 cgaactcttg atctgtaaca acccactcgt catctccacc acttcgccgt aaatcacact
     1321 gccgtcggtc aaggtcacga cgtccagagc cgcagccggc gtcatcgggt ccggtccctc
     1381 atcggcaaaa gcagggtgcg ccatcgtgat cagaccaagg attgcgacca atatcacaga
     1441 ccgcttcatt tattctcctt cgtgcagatg aataacttat ctacagttaa caaacctaag
     1501 tacctatcgt gtttgcgaac gacagtgcaa gacgctcatc ccgttcattc ccttgttggt
     1561 gtttagggac gacgacatcg acgaattgcc ggctagcggt cgttcaccat acggacggta
     1621 aggcttgagg aggggagcgt gatccctcgt tcttgaaatc gttggagaat taatttgttc
     1681 aactctccct gagtcgagct gtaatcggcc gcagccgtcc aaggttctac ggcaatagcc
     1741 accatcagag aagactcccc aagagatgac actcctactg aaggaaccgg gtctttcatg
     1801 accgatgggt gtcgatcaag gacatcccga acttgaacaa tggcctcatc gagattcgcg
     1861 ttcggtgaca caggaatagt caaatgcagc tggcggatag ttccgaaatt gtgcaatatt
     1921 tctccgacga ttttccgatt gggaatgatg acgcgggaac gatccgcatg catcagcgtt
     1981 gtggtgaaga tgtcgatcat cacgacgtca ccgtggacac tgagcaacga aatatgctcg
     2041 ccgaccttat agggcttgct gaagatgatc gagagcccgg ccatcacgtt gctcaacaca
     2101 ccttgcaagg ccagaccaat acccactccc gcgactccga ggccggcgat cagaggggca
     2161 atgggcacac cgagcgtctg gagggcgatc atcgcggtaa acagcatcac cacgattttg
     2221 acgatgcgaa ccaggagcag ccgcaccggg ggctcaaggg tctgccgttc cagcgtctgc
     2281 tgagcaaagt taccggccca gcgcgacgcc atgctgcccg cgacgaggat gccgaaggcg
     2341 accgccgcct gcagcccata ctgcacggca tagtgagtga gcgtatcaac gacagtcata
     2401 gggatcgtct ccttctagag gctacgttga atacttgaac cgtacgagaa ggccgggagg
     2461 tagggctatc accgcgtcct gctatcgtcc gaagagtcct ttcagaagct cctgtccctc
     2521 tttcttcaag tcctccggtg ttttcgtacc tttgatcaat ccatcgaccg cttcttccac
     2581 cttcttccgt acttgttgat gcacttttcc tgtgacgcct ttgagatcaa ctccatacgt
     2641 cggcgcctgc gcggtgccgg taatggtgag cggaagactc aaccggccgt ctttgacggc
     2701 aagtttcacg acaggtgacg cggcggcgat tttccggctc agatcctggg ataggttgag
     2761 attcacgagg agattcaatc gctgatcaaa tccgatggta ccgccgccgg tggcctgaaa
     2821 atcatggctg tccatcaaca gatgctgtac gttgatgatc ccctgcttga tggcgagatc
     2881 cgtttcgatc gtagaaaatg cagtggcctt ggcattgtcg agcgaaatgc cggctacctt
     2941 gagagccgag accacttcct gcagaagatt caccccttca atcttcccat ccttcaccgt
     3001 catgtgcccg gttccttcca atgccgttgt cagatccggc atagagaatc ctatgccttg
     3061 cagcgagagg tcggcaccgg ctgttccgct gacggagatc ggcgcctcgg caacggcatc
     3121 gagcgccgga ccgagctgca ctccctgaac cgtgacaccg cccttgaaag gcggcgcgtc
     3181 gggtccggcg atcagtttgc cctgcccttt catctggccg tcgaatagtt ggaacgacaa
     3241 ggtattcagc ttgatccctt gtcctttcac ttccgccgcg atctggaaat tcttaatgtc
     3301 gaccggcttt ttcaggggaa gcgagaccgg aaggttcgca gtagtgatca ccggcgaagt
     3361 gatgtagaca ttggcgtcgt tcccggcggc attgcctgta atagtaaagt cggtcttccc
     3421 catacccagt tcgaaattaa tcgcctcgat gtccatcgtt tctctcagcg ggccgaactc
     3481 accgtcgagt ttcaccggca gattgaaggg ctgcacgagc gagaggaaat gtagacgtgg
     3541 agttcg
//
LOCUS       DATNDD010000289         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_95730, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000289 DATNDD010000000
VERSION     DATNDD010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95730"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VN647_06915"
     CDS             complement(<1..208)
                     /locus_tag="VN647_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96799.1"
                     /translation="MAKILCVLYPDPETGYPPTYARNDIPTITTYADGQTAPTPKGPL
                     GFQPGELVGSVSGELGLRHYVEKLG"
     gene            complement(341..1129)
                     /locus_tag="VN647_06920"
     CDS             complement(341..1129)
                     /locus_tag="VN647_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96800.1"
                     /translation="MEHTSSSLRCRYYRRWWVFAGVMILHAPCALGQMMLLPDDRVIV
                     GTVTDIRSDQVQVKMDADDVEPRYLSAKEAQEKGVWPLKKGDRLHIVVNEQNVTLGYH
                     RVGDSGFHQIVRGRLAQPLVVDQEWAVVQRDGGGEHAYRVRPLIRSKMAGVPIRQPAV
                     FLIDETDQIMDVMFGGEETLRTAIKGWHGSPPKSVDQQLRGTIVKPVADNRVMIRTED
                     GAERVIEVRAFVQDKLKKLPAGKSVILLLDGENKVTDLAVSPVP"
     gene            complement(1176..1934)
                     /locus_tag="VN647_06925"
     CDS             complement(1176..1934)
                     /locus_tag="VN647_06925"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HWG96801.1"
                     /translation="MDKLIVITFTEENRAVDALMSLRRLRDQGEIEFYDAGIIARGKD
                     GKVSMKQVTDKMPEEFMWGSLIGSFIGLIGGPVGVVAGSIAGGLTGATLDFVDVGVKK
                     DFIDQVGQSLQANNSAVVALVWEDSNESLDQAMAPLGGRVFRTGIRDELEKQIEAQVK
                     ADKGTETRLKTELAQAKAEVKAKVQQELDGLRAKKEALRKELDARKQRIEANIEAVKR
                     KVSGVVGEMKAKLEQRIALMKERQDRQDKLQALL"
     gene            complement(2060..2452)
                     /locus_tag="VN647_06930"
     CDS             complement(2060..2452)
                     /locus_tag="VN647_06930"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWG96802.1"
                     /translation="MKPIMSIIPLVIAGLVVSGQTHAQVAGSSTLGVSMTEIKDVAMG
                     WSAKKQVLGQPIYNEKKDKVGTIDDIIIAPDKAVSYVIVGAGGFAGLGKHDVAVPVNQ
                     VKEEGSKFILQGATKEAIKAMPKFEYAR"
     gene            complement(2502..>2890)
                     /locus_tag="VN647_06935"
     CDS             complement(2502..>2890)
                     /locus_tag="VN647_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWG96803.1"
                     /translation="DKGNVTLFGEVASPEEKGWAETIAGTVPGVLSVRNSVIVDVALA
                     PDYKIRRDVWEAFKQTPALEKNPTVRISARQGEVTLKGDVLDDRSKNAAEKAALGVQG
                     VKKVISHLQVVDRLPPQKLDNREIIR"
BASE COUNT          540 a          893 c          724 g          733 t
ORIGIN      
        1 ggccgagctt ctccacatag tggcgaagac ctagttcgcc tgagaccgat ccgacgagct
       61 cgcccggttg aaatccgaga ggtcctttcg gtgtgggcgc ggtttggccg tcggcatagg
      121 tggtgatggt cgggatgtca ttccgcgcat aggtcggcgg atatccagtt tctgggtcgg
      181 gataaaggac acataaaatt ttcgccacgg ggatgtttcc tttccttctc ctggctgcac
      241 actgtacggt tgccaatgac gcgtataagt cacgaactca tctgacagat aatgagccct
      301 catcaggttt acgtcgctga catttcaccc gcctagcccc tcaaggaacg ggagaaacag
      361 cgagatccgt caccttgttt tcaccatcca gtaacaaaat caccgacttc cccgccggga
      421 gcttcttcag tttgtcctga acaaaggcgc ggacctcgat cacacgttcc gcgccgtctt
      481 ccgtccgaat catcacccgg ttgtcggcga ccggcttcac aatggtccct cgaagttgct
      541 gatcgacgct tttgggcggg gagccatgcc agcccttgat cgccgtccgg agtgtctctt
      601 caccgccaaa catgacatcc atgatttgat ccgtctcatc gatcaagaac acggcaggtt
      661 gtctgatcgg gacgcctgcc atcttgctcc ggatgagtgg gcgcacacga taggcatgct
      721 ctcctccgcc atcacgttgc accactgccc attcctggtc tacaaccagc ggctgcgcca
      781 gccggccccg cacgatctga tggaagcccg aatctccgac gcggtggtac ccgagcgtca
      841 cattctgctc attgacgacg atgtgcaatc gatcgccttt cttcagcggc cagacgcctt
      901 tttcttgcgc ttccttggcg gacagatagc ggggttccac atcatccgca tccatcttga
      961 cttgaacctg atcgctgcgg atgtccgtga cggtgccgac gatgacgcga tcatccggca
     1021 acagcatcat ctggcccagc gcgcacggcg catgcaagat catcacgccg gcaaagaccc
     1081 accaccggcg ataataccga catcgcaatg acgacgatgt gtgttccatg agttcaacct
     1141 ccatcgtctg aaaatcagga accgatcaat tgcaattaga gcaacgcttg tagtttgtcc
     1201 tgtctgtcct gcctttcctt catcagtgcg atgcgctgtt cgagcttggc cttcatctcg
     1261 ccgacaacgc cgctcacttt gcgttttacc gcctcgatat tcgcctcgat ccgttgcttc
     1321 cgtgcgtcaa gttcttttcg gagcgcttcc tttttggcgc gaagaccgtc gagttcttgt
     1381 tgaaccttgg ctttcacctc ggctttcgcc tgagcgagtt ccgtcttcag tctcgtttcg
     1441 gtccctttgt cagccttgac ttgggcttca atctgttttt cgagttcatc cctgattccg
     1501 gttcgaaaca cgcgtccgcc gagcggtgcc atcgcttgat cgagagactc gttcgagtct
     1561 tcccagacca atgccacaac ggcggagtta ttggcctgca gtgactgacc gacttggtcg
     1621 atgaagtctt tcttcacacc gacatcgacg aagtctaggg tggcgccggt cagccccccg
     1681 gcgatgcttc ccgcgacaac accaaccggt ccaccgatca agccgatgaa gctgccgatc
     1741 agcgagcccc acatgaattc ttccggcatt ttgtcggtga cttgtttcat ggagactttt
     1801 ccatctttcc ctctcgcgat gatccccgca tcgtaaaatt cgatctctcc ttgatctcga
     1861 agacgacgca gagacatcaa cgcatcaacc gcgcggtttt cttcagtgaa tgtgattacg
     1921 atcagcttat ccatggtttc ccttcttttc attccggtgt gagagctttc gatcacgtga
     1981 cattaagccc acgccgtcca ctcgtccgct ggtcgggtgc cgggcttcca ccacaggatc
     2041 gatcattcga catgttcgct tatcgcgcgt attcgaattt cggcattgct ttgattgcct
     2101 cctttgtcgc gccctgcagg atgaactttg agccttcttc cttgacctgg ttgaccggaa
     2161 ccgcgacatc gtgcttgccg agtccggcga acccgccggc cccgacgatc acataggaga
     2221 ccgctttgtc gggcgcgatg atgatgtcat cgatggtgcc gactttgtct ttcttctcgt
     2281 tgtatatggg ttggccgagc acctgtttct tagcgctcca tcccatcgcc acatccttga
     2341 tctccgtcat cgagacccca agtgtgctcg atccggcgac ctgagcatgt gtttgtcctg
     2401 acactacaag cccagcgatg actaaaggta tgatggacat gatcggcttc atcgtgccgt
     2461 ctcctttcct caaaagaaag agcgatgacc gccgccggcc gtcagcggat gatttcacgg
     2521 ttatcgagtt tctgcggagg taaccgatcc accacctgca agtgactgat cactttcttg
     2581 accccttgga cgcccagcgc cgctttttcg gctgcattct tcgagcggtc gtcgagaaca
     2641 tcgcccttga gagtcacctc gccctgacgc gcggagatcc ggacggttgg attcttttcg
     2701 agcgcaggcg tctgcttaaa agcttcccag acatctcgtc tgatcttgta gtccggtgca
     2761 agtgccacgt ctacgatcac gctgtttctc acgctcagca cgcccggtac tgtgccggcg
     2821 atcgtctcgg cccagccttt ttcctccgga cttgcgactt cgccgaataa cgtgacgttg
     2881 cccttgtcga
//
LOCUS       DATNDD010000290         3243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2028125, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000290 DATNDD010000000
VERSION     DATNDD010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028125"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="argB"
                     /locus_tag="VN647_06940"
     CDS             <1..498
                     /gene="argB"
                     /locus_tag="VN647_06940"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWG96804.1"
                     /translation="PLTAKAWAESLDRELDGEDGDGDFGLVGDIEKVDPGLLRNLQDD
                     HYIPVIAPIGTDREGNTYNINADLVAGAVAGALRAEKLLMMTDIKGIRDANGRHLSTV
                     SRKDVQRMMKKGTITEGMIPKVHACLDALAEGVGKAHIIDGRIPHALLLEIFTHKGIG
                     TEIVN"
     gene            524..1390
                     /locus_tag="VN647_06945"
     CDS             524..1390
                     /locus_tag="VN647_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWG96805.1"
                     /translation="MPIDRNHLKEDLYRLVGERHPLSSPLLLQEAEAYLHRQFSETGL
                     TVTKQPFQALGGTYHNVIGTALPAVQPSQSAPPLILAAHFDTVQGSPGADDNASALAV
                     LLSITRQVRAMTLGRPIRFIAFNLEEDNLLGSSAYTALLRKNREAIHGAIVLECVGYA
                     SHQNNSQKIPPGVPIAVPTTGNFLAVIGNEQSRALTGAVAQSMKSHLPIVPLVVPGNG
                     EKLPDTRRSDHTSFWEQGFPAVMLTDTANFRNPHYHRPTDTLDTLNLDFIASVADGVI
                     AAVIALAGQPTA"
     gene            1511..>3243
                     /locus_tag="VN647_06950"
     CDS             1511..>3243
                     /locus_tag="VN647_06950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWG96806.1"
                     /translation="MPTLNWIGKDAVVNHHHQVPFHLLKDVPDLACGDPGSGNLIVQG
                     DNLVALKALLPYYAGQVKCIYIDPPYNTGNENWVYNDNVNSPLIREWLGKVVGKEGET
                     LDRHDRWLCMMYPRLALLRQFLREDGVVLVSIDDSEVTNIRILLDDIWGRENFIAQLI
                     WEKGRKNDAKFFSVGHEYIIIYARSISRLRQENTIWREEKPGAKAIHDEYLRLKTIHG
                     NDLKSIETALRQFYKMLPKGHPSKKHSRYGNVDDDGVWRDDNMSWPGGGGPRYDVTHP
                     ATGKPCAVPDGGWRYATLGKMQEMIARGVVVFRETDKEPPIRKTYLVRGDDDTDDIDE
                     NDIGRQVMGSYFYRSALQASNTLQGLFGLKMFENPKDHEVLSRLIRYVSNDDRESIIL
                     DSFAGSGTTGHAVISLNKQDDGNRRFILVEMELKIAQEITAERVRRVAEGYKNAKGEK
                     VEGLGGGFRYCELGEPLFDQSGKIRESVTFAELARHFYFTETGEPLPRERVSKSPFLG
                     ECRGVGIYLLYNGILDDKSVKGGNVLTRTVLAKLPKFDGQKVIYCAGCLLGKDRLQAE
                     RILVHQTPYEI"
BASE COUNT          794 a          865 c          877 g          707 t
ORIGIN      
        1 ccgttgacgg ccaaggcctg ggcggaaagc ctcgatcgcg agttggacgg agaagatggt
       61 gacggcgact tcggactggt gggtgacatc gaaaaggtcg accccggcct gctgcgtaat
      121 ttacaggacg accactacat ccctgtgatc gctccgatcg gaacggatcg cgaaggcaac
      181 acctacaata tcaatgccga tctggtcgcc ggagccgtgg ccggggcctt gcgagcggag
      241 aaactcctca tgatgaccga tatcaaaggc attcgtgacg ccaatgggcg ccacctgtcc
      301 accgtgtcgc gcaaagacgt gcagcgcatg atgaagaagg gaaccatcac ggagggaatg
      361 atcccgaaag tccatgcgtg tctggacgcg ctggccgaag gagtcgggaa agctcacatt
      421 atcgacgggc gcattccaca cgcccttctt cttgaaatct tcacgcacaa gggcattggg
      481 accgagatcg tgaactaata gcttcccttg atggaccaac cttttgccga ttgatcgcaa
      541 ccacctcaaa gaagacctct atcgcctcgt cggtgagcgc catcccctct cttctccgct
      601 tctccttcag gaggcagaag cctatctaca ccgacagttc tccgagacag gtctgaccgt
      661 cacgaagcaa ccctttcaag ccttgggcgg cacctaccat aatgtgatcg gaacggcact
      721 tcctgccgtt cagccatctc aatccgcccc gccgctgatc ctcgccgcgc acttcgacac
      781 agtacagggg tctcccggcg ccgatgacaa cgccagcgcc ctcgcggtac ttctatccat
      841 tacccgccaa gtccgagcca tgacattggg cagaccgatc cgtttcatcg cttttaactt
      901 ggaagaagat aacttgctcg gcagctcagc ctataccgcg cttctgagaa aaaatcgtga
      961 agccatccac ggcgcgatcg tgttggaatg tgtcggttac gcaagccatc aaaacaactc
     1021 gcagaaaatt cctcccggtg tgcccatcgc tgttccgacg accggcaact ttctggccgt
     1081 gatcggcaac gagcagtcac gggctttaac gggcgccgtc gcccagtcga tgaagtcgca
     1141 cctcccgatt gttccactgg ttgtgccagg caacggagag aaactcccgg acaccagacg
     1201 cagcgatcac acctcgttct gggaacaagg gtttcccgcc gtcatgctca ccgacaccgc
     1261 aaacttccgg aatccccatt accatcgtcc gaccgatacc ctcgacacgt tgaatctcga
     1321 cttcattgcc tcggtagctg atggggtcat agcagcagtc attgcattag ctggtcagcc
     1381 gactgcataa ctttcattgc tgcgtagctt tcagagaacg atttcgctaa acttccggcc
     1441 gacagacggc cctacctttg cgctagagca cgacatccga tgaaagcatg aacacccaac
     1501 ccatcttgtc atgcccacgc tgaactggat cggaaaagac gcggtcgtca atcaccatca
     1561 tcaggtgccc tttcatctgt tgaaagacgt gcccgacctc gcttgcggcg atccggggtc
     1621 gggcaatctt atcgtgcaag gcgataatct ggtcgcgctc aaggcactgc ttccctacta
     1681 cgctggacag gtgaagtgca tctatatcga cccgccgtac aacacaggca atgagaactg
     1741 ggtgtacaac gacaatgtga acagcccgct tatccgcgaa tggctcggca aggttgtggg
     1801 caaggaaggt gagaccctcg atcgccatga tcgttggctc tgcatgatgt atccacgttt
     1861 agcattgctt cggcagtttc ttcgggaaga tggagtcgtc ttagtgtcta tcgacgactc
     1921 agaggttaca aacatacgga tactattgga tgatatttgg ggtcgagaga atttcatcgc
     1981 acaactgatt tgggagaagg ggcgtaagaa tgatgccaag tttttttcag ttggacatga
     2041 atacataatc atttatgcca ggtcaattag tcggttgagg caagagaata ctatctggcg
     2101 agaagagaag cctggtgcaa aagcgattca tgacgagtac ctccggctaa aaaccattca
     2161 cggcaatgat ctgaagtcca ttgaaacggc cttacgtcag ttttataaaa tgttgccgaa
     2221 gggacatccg tctaaaaaac actctcgata tggcaatgtc gatgacgatg gtgtttggcg
     2281 cgatgacaat atgtcttggc caggtggagg agggcctagg tatgacgtaa ctcatcctgc
     2341 gacaggcaaa ccatgcgcag tgccggatgg cggctggcgt tatgcgacac ttgggaaaat
     2401 gcaggaaatg attgccaggg gagttgtggt atttcgtgaa actgacaaag agccgccgat
     2461 tcgcaaaacc taccttgtca gaggtgatga tgataccgat gacatcgatg aaaatgacat
     2521 cggcaggcag gttatgggca gttactttta tcgaagtgcc ttacaggcat caaatacact
     2581 ccaagggctt tttgggctaa agatgtttga gaatcccaag gatcatgaag ttctatccag
     2641 gctcattcgc tatgtatcta atgacgaccg ggagtcgatt atattggact ccttcgcagg
     2701 ttctggaaca accggccatg cggttataag ccttaataaa caggatgatg gcaatcgacg
     2761 cttcatcctg gtcgagatgg agctcaagat cgcgcaagag atcaccgccg agcgggtgcg
     2821 gcgagtggcc gaggggtaca agaacgccaa aggcgagaag gtggaagggc ttgggggtgg
     2881 attccgctat tgcgagttgg gcgaaccgct gttcgaccag agcggtaaga ttcgggagtc
     2941 cgtcacgttt gccgaactgg cgcggcactt ttactttacg gaaaccggag agcccttgcc
     3001 tcgtgagcgt gtgtcgaaat cccccttcct tggcgaatgc cgtggagtag gaatctatct
     3061 gctctacaac ggcattctcg acgacaaaag cgtcaaaggt ggaaatgtcc tcacgcggac
     3121 agtgctggcg aagctgccga agtttgacgg gcagaaagtg atctattgcg ccggatgtct
     3181 gctgggcaaa gataggttgc aagctgagcg cattctcgtt caccagactc cttacgaaat
     3241 caa
//
LOCUS       DATNDD010000291         9037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_510248, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000291 DATNDD010000000
VERSION     DATNDD010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510248"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VN647_06955"
     CDS             <1..1310
                     /locus_tag="VN647_06955"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWG96807.1"
                     /translation="EPERVVLDNGMVVYLLEDHELPLVTITVTMRTGSWLDPTDKIGL
                     AGMAGAVMRTGGGGGLSAEQVDTELEQFAGEVSIGISRQSGSASLDVLSKDVNRGLEI
                     FVGLIRTPTFDPSRVELAKLQAIEGIRRRQDNPGSVVGREFVKMLYGADHPSARESSV
                     DSIKRITRDDLVTFHRNTIHPNGMILGVTGDFKRDEMIASLRKMFGDWEKGTVPELRI
                     SDVPTEESSRSAVRFVGKDTSQTHLRVGHLSIKENDPDYVALAIANDILGGSSFRSRL
                     FNDVRSKRGLAYSVGSRLNTGMHDQGIWQMRAETKLISTQEVIERFVANIERMRVEPV
                     TDAELAEAKEAYVNSFVFSFSSPSAIVSRLVELEYDGLPKDFLQQLRAKVVKLTKDDV
                     LAAAKKHLHPGRLKIVAVGSGEALPKALATFGDVKEIKLKPEG"
     gene            complement(1404..1781)
                     /locus_tag="VN647_06960"
     CDS             complement(1404..1781)
                     /locus_tag="VN647_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96808.1"
                     /translation="MKVKKLKVGVRSLEEGLQEFGATLKAVQSGARPFPNERVMYFTS
                     VEAMRQVLTPSRLTLFHCICTRHPRSIAALAKLILMDFKNVHADLKLLTDLDLVEFQS
                     GAHLRDSATPTIPYERIQFEIAV"
     gene            2037..2879
                     /locus_tag="VN647_06965"
     CDS             2037..2879
                     /locus_tag="VN647_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWG96809.1"
                     /translation="MSLEDDLSDILKKARTGQGLSVGDVARMTGLPGGDITALERGDQ
                     PRDRAEVRALAKALGLRAEPLEQIAIDKWEPVAQHMPPWVDMVHGSINGYGVQGYVVH
                     DEGEALLVDTAYNASAMLDLLRRRGMRLVGICLTHGHADHADGIEQILDSHEVPVYLG
                     SEDISLLSWRPRTDLLVTPSDGMSISVGRRTVHCVTTPGHTPGGICYRLDEAQHPVCF
                     VGDTLFAGSIGRSNPKELYSTHLNSVRRRVLTLSPDYRLFPGHGPATTVEEEVDHNPF
                     GTIQ"
     gene            2891..3871
                     /locus_tag="VN647_06970"
     CDS             2891..3871
                     /locus_tag="VN647_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWG96810.1"
                     /translation="MDGFGRHEHRDLDRGLVMDAGEERVPQLVDQIESDVDEEPSFFS
                     KWTLPIVLFLMTLFTTLWAGAYQAYTGPVRGPLNFLLASPEMLWRGIPFAGALLFILT
                     THELGHYLLSKIHRVPASLPLFIPGPPHFIGTFGAIIRMRGPILSRRALFDIGVAGPL
                     AGFAVAIVAMIVGLNLSTVVDRTATYGLQLGEPLLLQLMSWVVIGPIPPEADVVLHPI
                     GFAAWFGLFVTSLNLLPIGQLDGGHVAYALWGPRQRTMALAFLPILLVLGFVGWSGWF
                     LWAFMAGLWGVGHPPVMDPHVSLGRNRTIVGWIAFAVFVVTFAPVPFSFH"
     gene            complement(4136..4993)
                     /locus_tag="VN647_06975"
     CDS             complement(4136..4993)
                     /locus_tag="VN647_06975"
                     /inference="COORDINATES: protein motif:HMM:NF020584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HWG96811.1"
                     /translation="MEAIITESRKAFYKALVNVLLIDTAGVPSNADKDNPLSVRIASG
                     IAKLIEAESRGTRLAAQASGSKFEEICLTFLTNTFLRLKHLRPGQWKIARVPGRSRLA
                     LASYEQYSHLTAIAEATKNNRQLAAAIGSDYLIAPDIVISREPEEDAVINEAGQLVDD
                     KSVKLSALRKSNKCLPTLHASISCKWTIRSDRVQNARSEALNLIGNRKGHLPHVVVII
                     GEPLPSRIASIALGTGNIDCVYHFALDELNETVHKLKNEEGTKETLQTMIEGKRLKDI
                     SDLPLDPAL"
     gene            5331..5495
                     /locus_tag="VN647_06980"
     CDS             5331..5495
                     /locus_tag="VN647_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HWG96812.1"
                     /translation="MCQTERFQPVFANDFNAYAAKTYNANFGDHCLHGDINDILANGS
                     FKFPEAEIVM"
     gene            5492..6337
                     /locus_tag="VN647_06985"
     CDS             5492..6337
                     /locus_tag="VN647_06985"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HWG96813.1"
                     /translation="MIGGPPCQGFSPLNKQREGDPRKQLWGAFMEVVRRINPVAFVME
                     NVPELLSSAEFESIKDHAEAMGYFVTSGVLNAADYGVPQRRKRAIVLASRLGLLSLPT
                     STHCNPQKNPTLFQNGMLPWETVKRAIYDLPVPEGIELRAGDIPPPLDLHFGRTPTKQ
                     SLARYRCVPEGGNRFDLQKKRPNLTPECWIKKRSGGTDLFGRLWENRPAFTIRTEFFK
                     PEKGRYLHPIQHRPITHREAARLQSFPDSFKFTGTKIEIAKQIGNAVPPLLGKRIAEV
                     LIDHL"
     gene            7232..7534
                     /locus_tag="VN647_06990"
     CDS             7232..7534
                     /locus_tag="VN647_06990"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWG96814.1"
                     /translation="MANISADQQLVAEFCRQRHIRRMAFFGSVLRADFGPHSDIDVLV
                     EFEPAHVPGLFGIARMERELSTLFGGRNVDLRTPEDLSRYFREDVLKEAEVQYAQG"
     gene            7521..7865
                     /locus_tag="VN647_06995"
     CDS             7521..7865
                     /locus_tag="VN647_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HWG96815.1"
                     /translation="MRKDDDVRLRHMLDAAREALGFVQGRNRADLNSDRQLVLARVKA
                     IEIIGEAAFRTSENARAQLPEIPWEDIVGMRHRLVHAYFDINLDVLWQTVQGDMAPLI
                     SRLEQVLDTKRN"
     gene            8130..8711
                     /locus_tag="VN647_07000"
     CDS             8130..8711
                     /locus_tag="VN647_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96816.1"
                     /translation="MTAKHWLIESDTATDSMTLYGRAAVLTAMIWWGLKFIVTHLETN
                     YTGESFLHLVNLPFHEAGHLIFIPFGRFMTILGGTLGQILMPMICLGTFLVKTRDPFG
                     ASVALWWTAESLMDIAPYINDARALDLMLIGGVTGKETDGHDWNNILTMLNLLEWDHR
                     LAHFTYNVGILLMLASIVWGGWLLLRHYRRLSA"
BASE COUNT         1948 a         2280 c         2540 g         2269 t
ORIGIN      
        1 tggagccgga acgggtcgtt ctggacaacg gcatggtcgt ctatttattg gaagaccatg
       61 aattacccct ggtcacgatc acggtgacca tgcgaacagg tagctggctc gatccgaccg
      121 acaagatcgg actggcgggc atggccgggg cggtgatgcg gaccggtggt ggcggcggtc
      181 tttccgcgga gcaggttgat acggaactgg agcaatttgc aggcgaggtc agcatcggaa
      241 tcagcagaca atccgggtcg gcatctctcg atgtcctgag caaggatgtg aatcgggggt
      301 tggagatctt cgtcggtctc attcggactc cgacgttcga tccctcccgt gtcgagctgg
      361 ccaagttaca ggccatcgag gggatccgcc gccgtcagga taatcccggc tccgtcgtcg
      421 gccgagaatt tgtcaagatg ctctatgggg ccgatcatcc gagcgcgcga gaaagctcgg
      481 tggactcgat caaacgcatc accagggacg atctcgtcac gttccatcgc aacacgattc
      541 acccgaacgg tatgatcctc ggtgtgaccg gtgacttcaa gagagacgag atgatcgcct
      601 ctctccgtaa gatgttcgga gattgggaaa agggaaccgt gccggagctg agaatttccg
      661 atgtgccgac ggaggagtcg tccagatctg ccgtcaggtt cgtcggtaaa gatacctcgc
      721 agacccatct tcgagtggga catctttcga tcaaggagaa tgatcccgac tatgtcgcat
      781 tggccatcgc gaacgatatt ttgggtggca gttcattccg tagccgcctg ttcaacgatg
      841 tgcggtcgaa acgggggctg gcctattccg tcggcagccg cctcaatacg ggcatgcatg
      901 accaaggtat ctggcagatg cgggcggaaa ccaagttgat ttccacccag gaagtgatcg
      961 agcggtttgt ggcgaatatc gaacggatgc gcgtcgagcc ggtgaccgat gccgagcttg
     1021 cggaggcgaa ggaagcgtac gtcaattcgt tcgtgttttc attttccagc ccttcggcca
     1081 tcgtcagccg gttggtcgag ttagagtacg atggattgcc gaaggacttt ctccaacagc
     1141 tgcgcgccaa ggtggtgaag ctgaccaagg atgacgtctt agcggcggca aagaaacatt
     1201 tgcacccggg tcgtttgaag atcgtcgccg tcgggtcagg ggaggccttg ccgaaagccc
     1261 ttgcgacgtt tggagatgtg aaagaaatca aactgaaacc ggaagggtga agaagttccg
     1321 tggctggtga agcgtggcca gctacggcgg cgccatgatg cgacggagcc gcctgatggc
     1381 ctgagacgat ttctcatgtg ggttcacacc gcgatttcga actgaattcg ttcataagga
     1441 atagtcggag tggcggagtc cctcaggtgc gcccctgatt gaaactccac cagatcgagg
     1501 tcggtgagga gtttcagatc ggcgtgcacg ttcttaaaat ccatgaggat cagcttggcc
     1561 agcgcggcga tagagcgagg atgacgagtg cagatgcaat gaaaaagggt cagtcgagac
     1621 ggcgtcagca cttgtcgcat cgcctctacg ctggtgaaat acattacccg ttcgtttggg
     1681 aacggtcttg ctccggactg gaccgccttc agcgtggccc cgaattcttg caggccttcc
     1741 tctaatgaac gcactcctac cttcagtttc ttgaccttca ttgtcccaac tccttcttga
     1801 ctgccgtgat atcgcgacga aagtcatatc gtatcaccct tcattacctt gatcaccaac
     1861 cacatggcgc ggtactgtga agcgtgtgag tacccgagga tggaatttca ccgaagtgac
     1921 agagtcatgg tactctttat tgggttgatc gatagtgcga gaatttctga atgaagggtt
     1981 tgctggtttc ggcaaaccta atgttgccgc tgatcggatt ctgataaggc tcgcgcatgt
     2041 cgctcgaaga tgatttatcc gacattctta aaaaagcacg caccggacaa ggattgtccg
     2101 tcggcgatgt tgcaaggatg acgggattgc ccggcggcga tattactgcg ctggaacgtg
     2161 gcgatcagcc acgagaccgt gccgaggtgc gtgccttggc aaaggccttg ggtctgcgcg
     2221 ctgagccgct cgagcaaatc gccattgata agtgggagcc ggttgcccaa catatgccgc
     2281 cctgggtgga catggttcat gggtccatta atggatatgg cgtgcaggga tatgtcgtgc
     2341 atgatgaagg tgaggcgttg ttggtcgaca ccgcctataa cgcctccgcg atgcttgatc
     2401 tgcttcgacg acgaggaatg cgccttgtcg gcatttgtct cacgcacggt cacgcggacc
     2461 atgcggatgg gatcgagcag attttagact cgcacgaagt cccggtgtac ctggggtcgg
     2521 aagatatcag tctgttgagt tggcgaccac ggacggattt actggttacg ccgagcgatg
     2581 gaatgtcgat cagtgtcggc cgccgtacgg ttcactgtgt gacgacgccg ggtcatacac
     2641 cgggcggtat ctgctaccgg ctggatgagg cacaacaccc ggtctgcttc gtcggtgata
     2701 ccctctttgc cggatcgatc ggccggtcca atcccaagga attgtattcg acccatctca
     2761 attcagttcg ccgccgtgtg ctgacccttt cacctgacta tcgtctcttt cccgggcatg
     2821 gacctgccac gaccgttgaa gaagaagtcg accataatcc gtttgggacg attcaataag
     2881 caggacttgc atggatggat tcggacggca tgaacatcgc gatctcgata gaggtctcgt
     2941 catggacgcc ggcgaagagc gggttccaca gttggtggac cagatagaaa gcgacgttga
     3001 cgaagaaccg tcgtttttct cgaagtggac gttaccgatc gtcctctttc ttatgactct
     3061 ctttaccacg ttgtgggcgg gggcgtacca agcctacacc ggtccggtgc gcgggcctct
     3121 gaattttctg ctggcctctc ctgagatgct ttggcgagga atcccctttg ctggagcgtt
     3181 gctctttatc ctcacgacgc atgagttggg gcattatctc ttgtccaaga tccaccgggt
     3241 gcctgcttcc ttgccgttgt tcattcccgg gcctcctcac ttcatcggga cattcggcgc
     3301 catcatccgc atgcggggtc cgattctgag tcgtcgcgcc ttgtttgaca tcggggtagc
     3361 cggtcccttg gcggggtttg cggtcgccat cgtcgcgatg atcgtcggcc tcaatctctc
     3421 cacggtcgtg gatcgaacgg ccacgtatgg attgcaactg ggggaacctt tgttgctgca
     3481 gttgatgtcg tgggtggtga tcgggccgat acctccggag gctgacgtcg tgcttcatcc
     3541 gatcggcttt gccgcctggt tcgggctctt tgtcacctcc ctcaatcttc ttcccatcgg
     3601 tcagctcgac ggcggacatg tcgcctacgc cttgtggggc ccgcgacagc ggacgatggc
     3661 gctcgccttt ctacctattt tattggtctt gggattcgtc ggttggtccg gctggtttct
     3721 gtgggcgttc atggcgggat tgtggggcgt gggtcatcct cctgtcatgg atcctcatgt
     3781 ttcgttgggg cgtaatcgga cgattgtcgg atggattgcg tttgccgtat ttgttgtcac
     3841 ctttgcgcca gtgccgtttt ccttccatta acaggtgcgt cgctactgct gccgggtgtc
     3901 gaacgacgta cacggacacc ggctatggga cgatccgtta agagcacgat tcctgtccct
     3961 tttgagttac cctcgcgctc aacttaaaga ccctcaaccc ccctttgtgt agtctcgacc
     4021 gcttcgtgta gtcggagtat cgaagctgcc cttatgacta tctgcatgat tcgatccgac
     4081 cctaatttgt gactccactc gagttgatgc cgaagtactc atggggtcag ccgggttata
     4141 gcgccgggtc gagcggaaga tcgctgatat ctttaagacg cttgccttca atcattgtct
     4201 gcagggtttc tttcgttcct tcctcatttt tcaatttatg aacagtttca ttgagctcgt
     4261 ccaaggcaaa gtgataaacg caatcaatgt ttccggtccc caacgctatg gatgcaatcc
     4321 ggctaggaag aggttctccg attatcacaa ctacgtgagg caagtggccc ttgcggtttc
     4381 ctattaaatt cagggcttcc gatcgggcat tttggactcg atcgctcctt atcgtccatt
     4441 tacatgaaat gctcgcgtga agcgtcggca agcatttatt gctttttctc agggcggata
     4501 gttttactga cttatcatcg acgagctgac cagcttcgtt aataacggca tcctcttccg
     4561 gctctctact tataactatg tccggggcga tgagataatc gcttccgatc gcagcagcca
     4621 gctgtcggtt attcttggtg gcttcagcaa ttgcagtgag gtgcgagtat tgctcatatg
     4681 aagccaatgc caatctggaa cggccaggaa ctctggcaat tttccattgt cctggtcgca
     4741 gatgttttaa tcgtaagaaa gtgttggtta gaaaggtgag gcagatttct tcgaatttgc
     4801 tcccggaagc ttgcgccgca agccgcgtgc ctctggattc ggcttcgata agcttcgcaa
     4861 taccggaggc gattcggaca ctcagaggat tatctttgtc ggcattggaa ggaactccag
     4921 ccgtatcgat taggagcacg tttacgagtg ctttgtagaa agcctttcgt gattcggtaa
     4981 ttatggcttc cattgagtca ctttaggtta ttctatatat tgaggttgac ttggaaaaac
     5041 aactcaatat actgtgccgt cacagggcgc gcgtaaccta tcactgattc caactcgcgc
     5101 gcaatcgaga tcaagatggc aaagaaactc ggtggcagtg agcaagtcaa agtccctcct
     5161 gacattagtc agattttacg tcggtatccc aatgaggcct atgctttact gaggcactct
     5221 ttcggaaaat taaccccagg cggtttcgct gctgagagca catcgtctta cggaaaaagg
     5281 aagttcagag tcatcgacct tttcgcaggc gcgggtggat ttacctagga atgtgtcaga
     5341 ccgaacgatt tcaaccggtc tttgcaaatg attttaatgc gtatgcggca aagacctata
     5401 acgcaaactt tggagatcac tgtctgcacg gcgatattaa cgacatcctg gcgaatggat
     5461 ctttcaaatt tccggaagcg gagattgtga tgtgattggt ggacctccgt gccagggttt
     5521 tagcccgctg aataaacagc gtgaaggtga ccccagaaag cagttgtggg gagccttcat
     5581 ggaagtagtc cgtcgaataa atcccgttgc gtttgtgatg gaaaatgttc ccgagttgct
     5641 atcctcggct gaatttgaat ccataaagga tcatgctgaa gcgatggggt attttgtgac
     5701 atctggcgtt ctgaatgcag ccgactatgg agtgccgcag cgcaggaaac gtgcaatcgt
     5761 tttagctagt aggctagggc tgctttccct cccgacctcc acgcattgta atccgcaaaa
     5821 gaatcctacg ctgtttcaga atggtatgtt gccttgggaa acagtgaagc gagccattta
     5881 tgatcttcct gtgccggaag gaattgaact gcgagcgggg gatatcccgc ctcctcttga
     5941 tctacatttc ggtcggacac ccactaaaca gagtctcgct cgatatcgat gtgtccctga
     6001 aggcggcaat cgatttgatc ttcaaaagaa gcggcctaat ttaacgcctg agtgttggat
     6061 taaaaagaga tcaggtggaa cagatctgtt cggccgtttg tgggagaatc ggcctgcctt
     6121 cacaataagg actgaatttt ttaagcctga gaaggggcga tacctgcacc cgattcaaca
     6181 taggccgata actcaccggg aagctgcccg cctgcaatct tttccggata gctttaagtt
     6241 cacaggaacc aagattgaga ttgctaaaca gatcggtaat gcggttcctc ccctgttagg
     6301 caagaggatt gcggaagttc tgatcgatca tctctgatgc attagtcccc tcgacttagt
     6361 agtcacagga aagggactgt ggctgacgtt tttaccaaga caaagcgttc agaaataatg
     6421 gctaggatca agggaaagca tacttctcct gaaatccggc tcgtgatgtt gctgaaaaga
     6481 ctgggatata acccacagcg acatagaaag gatctgccgg gttcacccga tgttgtttta
     6541 gcaagcgcaa agacggtgtt gtttgtaaac ggttgctttt ggcacggcca taagaactgt
     6601 aagagggcga cgctaccgag cagcaatgag acgttctgga tgagaaaaat agaaaagaat
     6661 atgcagcggg atgaacgtca gcgaagggct ctgagaaaaa tggggtggag cgtgctgacc
     6721 atttggacct gccggccggt atctctcgtc actttaccct ctctgttgag gcggggaggc
     6781 cttcgtaaga atcacagcga tacagcctgt cgaactaaac ggcaaaagtg aatgtataaa
     6841 gatcacagat atccttgcat tctgtggtgg cggaggagcc ggatcacatc gtcgttgtga
     6901 ctgtgtatac gttctacttt caggaaggag gcaaaccatg aaaatcgagt atgacaaaga
     6961 cgcggatgtc atgtatatcc gctccaagag ggcaactttc aatgccgcaa cgtacgactg
     7021 acggacgaga tcgcgctcga ctttgccgcg ggagaaaagt cggtcggcat tgagctgctt
     7081 ggtgcaagcc gtttattcga gaagcctgaa aatccggtga ttgaactaaa agacctgatc
     7141 cccaagatga tcgccgcata atgtgacaag tagcaccagt gacgggactt cttgttctca
     7201 agtggactag gaatgtaggg agagtggact gatggcgaac atatcggctg accaacaact
     7261 tgttgcggaa ttttgccggc aacgacatat ccgccgcatg gcctttttcg ggtctgtgct
     7321 gcgggccgat tttggccccc atagcgacat tgacgtactt gtagagttcg aacccgccca
     7381 cgttcctggc ctgttcggca tcgcgcgcat ggaacgtgaa ttgtccacac tgttcggcgg
     7441 ccgcaatgtg gatcttcgca cgcccgagga tttgagccga tatttccgcg aggatgtgtt
     7501 gaaggaggcc gaggttcaat atgcgcaagg atgatgacgt tcggctgcgg cacatgctcg
     7561 atgcggcgcg tgaagcgctc ggatttgtgc aaggccgaaa tcgcgccgat ctcaacagtg
     7621 accgtcagtt ggtgctggcg cgggtcaagg ccattgagat catcggtgaa gcagcttttc
     7681 gcacttccga aaacgcgcgc gctcaacttc cagagattcc ctgggaggac attgtcggca
     7741 tgcgccatcg cctcgttcac gcctattttg acatcaacct ggatgtcctc tggcagacgg
     7801 tgcagggtga catggcacct ctaatctctc gcctcgaaca ggtgttggac acgaaaagga
     7861 attgacggac gcttcattgc tttgtccgtt cgctcccccc tccgcattgt tcttctgatt
     7921 ttcttgtccg aattcacgcg cgatcctaca gtagagaaat gttctccacc ggtactccca
     7981 caatccgctg tccgaaatgt gaggctccac ggtcggatga ggccatggaa tgtatccaat
     8041 gcggcatcat ctttgccaag taccgaccgg atgccgggag agcttgcctc tcgtcatgcc
     8101 gaccgtcgcg gaggaagtcc gaatggctga tgacggcgaa gcactggttg atcgaatcgg
     8161 acactgccac cgattcaatg accctatacg gtcgagctgc tgtgttgact gcgatgatct
     8221 ggtggggttt gaagttcatc gtgacgcacc tggaaacgaa ttacaccggc gaatcgtttc
     8281 ttcacttggt caatctgcct ttccatgaag ccgggcattt gatttttatc ccattcggtc
     8341 gcttcatgac gattttggga gggactctcg gccaaatcct catgccgatg atctgcctcg
     8401 ggacattcct cgtgaaaacc cgcgatccgt tcggtgcttc agtggccttg tggtggacag
     8461 cggagagtct gatggatatc gctccctata tcaacgatgc acgagcgctt gacctcatgc
     8521 tgatcggcgg agtgaccgga aaagaaaccg atggtcatga ctggaacaac attctcacga
     8581 tgctgaactt attggaatgg gatcaccgat tggctcattt cacgtacaac gtcggtattc
     8641 tgctcatgct cgcctccatt gtctggggag gctggctttt gctgcgtcac taccggcgtc
     8701 tgtcggccta gctcaccacc cctcttcctg ctcaacatcg gcacactttc caacatcgca
     8761 ctgttgcgcg cgagttttta tcccttcttg acgattctcc cgaccatttt gacacggttt
     8821 ttacgcgccg tttgttaggc tccttaccga gaccgaaagg aggcaatatg gatctcgcgt
     8881 tcatcggatt agcaatcggt ttctttctcg tgtccggctg gcttatctcg gcgttggatc
     8941 gattgtaggg ggtatggcta tgaacggtat gtatctgctt ggcggcgtgc tctccctcgg
     9001 cctgctcgtc tatctcatgg tcgcgttact gaagccg
//
LOCUS       DATNDD010000292         3482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_523801, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000292 DATNDD010000000
VERSION     DATNDD010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523801"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..517)
                     /locus_tag="VN647_07005"
     CDS             complement(44..517)
                     /locus_tag="VN647_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWG96817.1"
                     /translation="MLKISKKADYALMALQHIASVQFGDVTPGRVVNTKEIAEEYNIP
                     LELLAKVLQALSKNGLIESHNGPKGGYLLARSARQITIAQILEGIEGPLGITDCSHEK
                     DGEFCMQREHCNIRTPLLKIQDSIYQLLNNMTLGDMLGGTPLITIQSPSAQGVER"
     gene            646..1062
                     /locus_tag="VN647_07010"
     CDS             646..1062
                     /locus_tag="VN647_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWG96818.1"
                     /translation="MGGTNPYIEKTDYELPKTSYSVTFIQPNGSSTTIPVDPESIPYG
                     ATGLPGSILDIAMGHGVDLEHVCGGVCACSTCHVIVKQGLETCNEGTDDEYDQLDEAP
                     MTTLQSRLGCQCVPNGTKDIVVEIPAVNKNLVREGH"
     gene            complement(1148..1852)
                     /locus_tag="VN647_07015"
     CDS             complement(1148..1852)
                     /locus_tag="VN647_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWG96819.1"
                     /translation="MAELTQPATVLAVTDLTPHVRQLVLKPKTSKISFQPGQWVSLKL
                     PVGPKPPLNRAYSMADPSSPYGELTLVFDHVPGGLGSNYLYELSSGDEVPLSGPYGNF
                     ILPQPLDRELLLIGRYTGLVPMRCLLKQMFFSKIQTPVLLIALAPNENEVLFHQEWLT
                     MALQYPSFRYLPLVAQNGESEAVEKTLAMLAPLVKGQPKVIPMLCGTKAFVRPLRAYF
                     TEGGYDRREVKIEAYD"
     gene            1979..3394
                     /gene="gltX"
                     /locus_tag="VN647_07020"
     CDS             1979..3394
                     /gene="gltX"
                     /locus_tag="VN647_07020"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWG96820.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQKGTFILRIED
                     TDQSRSTDESIQAIIEGMKWVGLDWDEGPFRQTERMDLYRSYALKLFETGNAYWCVCK
                     AEELEVRRKEAEAKGLSPRYDGRCRNLQITNPPGDAALRFKAPHEGQTVIDDLIKGKV
                     VFDNIVVDDVIILRSNGYPTYNFSVVIDDALMGITHVVRGDDHLTNTPRQILIFVALG
                     FPIPRFGHLPMILGADKTRLSKRHGATSIMAYKDMGYLPDAMVNYLVRLGWSHGDQEL
                     FTRQELIEKFSWDHVQKSAAVFNPDKLLWMNAEYIKTSPPRQVAQALVPLLEQAGLRK
                     EVEAVSDEWLAQLVVLVKERAKTLADMVEWVRPYFGQEIQFEAEAAKKFLTPAVAPLL
                     HKLLTRFEAFPSFSKPVWDESFKKLVEEEDIKMGALAQPVRVALTGRTASPGLFEVME
                     VLGRDRTLFRLQKGLERASMA"
BASE COUNT          825 a          828 c          998 g          831 t
ORIGIN      
        1 tccatgggag tggtggaatg gttatcgagg aaaataggaa gcttcatcgt tcgactcctt
       61 gtgccgaggg agactgaatc gtaataaggg gcgtgccgcc cagcatgtct cccaacgtca
      121 tgttgttcaa taattggtag atgctgtctt ggattttcag cagcggcgtg cggatgttgc
      181 agtgttcacg ctgcatacag aactcgccgt ctttttcgtg cgagcagtcg gtgatgccta
      241 gaggaccttc gataccttcg agaatctgtg caatggtgat ttgtcgggcg cttcgtgcca
      301 gaagatagcc ccctttgggg ccgttatggc tctcgatgag gccgtttttt gagagagcct
      361 ggagaacctt tgccaataac tccaggggaa tgttgtattc ttcagcgatc tctttcgtat
      421 tcaccacgcg gcccggcgtc acatcgccga actggaccga cgcaatgtgc tgcagcgcca
      481 taagcgcgta atcagctttt tttgaaatct tcaacattgc tattgccgat ctgtttgatc
      541 ggagactata atgatctccg atcagtgtgg tcggattaag agtagcatgc aggttccgct
      601 ggtgtcaata cacagttcag cgctgcctca gaaaggaata aaaccatggg cgggacaaac
      661 ccgtatattg aaaaaactga ttatgagctt ccaaaaacct cttactccgt gacatttatc
      721 caaccaaatg gaagttcgac cacaatccca gtcgatccag aaagtattcc ctacggtgcc
      781 accgggcttc ctgggagcat tttggatatt gcgatgggcc acggagtgga tttagagcat
      841 gtatgtggag gtgtatgcgc ctgttcgacc tgtcacgtca tcgtgaagca gggactggaa
      901 acctgcaacg aaggtacgga tgacgagtat gatcagctgg acgaagctcc catgacgacg
      961 ttacagtctc gcctggggtg ccagtgtgtg ccgaacggaa ctaaagatat cgttgttgaa
     1021 attccagccg tcaataaaaa tctcgtgcgg gagggacatt gatttgtccc gtcgcgtgtt
     1081 attcgttaaa catttaggtt aaacgcaaaa gaagagtcta acgaataacg attcacgaat
     1141 aacgtcttca gtcgtatgcc tctatcttca cctcccggcg gtcatatcct ccctccgtaa
     1201 aatatgcccg cagggggcga acgaaagctt tggttccgca cagcatagga atgacttttg
     1261 gctgtccttt gaccagcggc gcaagcatgg ccaaggtctt ttcaacggct tcggactctc
     1321 cgttctgagc gaccagcgga agataacgga aagagggata ttgcagggcc atcgtgagcc
     1381 attcttggtg gaacaagacc tcattttcgt tgggtgccag ggcaatcaat aagacgggag
     1441 tttggatttt cgaaaaaaac atctgcttga ggaggcatcg cattgggaca agaccggtgt
     1501 agcgtcctat cagcagcagc tctcgatcga gtggttgagg taggatgaaa tttccgtaag
     1561 gacccgacaa gggaacttca tctcctgagc tcaactcata gagataattg gagcccagtc
     1621 cgccgggcac gtgatcaaat accagcgtta actccccata gggcgacgaa ggatcggcca
     1681 tggaataggc gcgattgagg ggcggctttg gccctaccgg aagcttgagg gagacccatt
     1741 gtcccggttg aaaggaaatc ttgctggtct tgggtttgag cacgagttgg cggacgtgag
     1801 gtgtgagatc ggtaaccgca agtaccgtgg caggttgcgt gagctccgcc atgatcgatt
     1861 cgcagtaccg tcatagcaga agccatcagg agatggaagt ggcaagacga tgtacaagga
     1921 ttttcactgc atggtataga tgtcggtctg ttatgagtga agaagggacg gcacggtcat
     1981 gagtcaggtt cgcgtccggt ttgcccccag tccgacggga tttctccata taggaggagt
     2041 gcgcacggct ctgttcaact ggctctttgc gcgccagcag aaggggacct tcattcttcg
     2101 catcgaggat accgatcaaa gtcgctcgac ggatgaatcg attcaggcca tcatcgaggg
     2161 catgaaatgg gtcggccttg attgggacga gggaccattc cgccaaacgg aacggatgga
     2221 tctctatcgt agctatgcct tgaagttgtt tgagacgggg aatgcctact ggtgtgtgtg
     2281 taaagcggaa gaattggagg ttcggcgaaa agaagcggaa gccaaaggac tctcacctcg
     2341 ctatgacggc cgctgccgca atttgcagat caccaatcca cccggagatg ccgcacttcg
     2401 attcaaggcc ccgcacgagg gccagacggt tattgatgac ctcatcaagg gtaaggtggt
     2461 ctttgataat atcgtcgtag acgatgtgat cattctcaga tcaaacggct atcccaccta
     2521 taatttttca gtcgtgatcg acgatgcgtt gatgggcatc acgcatgttg tgcggggaga
     2581 tgatcatttg accaatacgc caaggcagat tctcatcttt gtggcgctgg gctttcccat
     2641 cccgcgcttc ggacatctgc cgatgatttt aggggccgac aaaacgcggc tctccaaacg
     2701 tcatggcgcc acctcgatta tggcgtataa agacatgggc tatctgcccg atgccatggt
     2761 caattatctg gtccgacttg gctggtctca cggcgatcaa gaactcttta cccggcagga
     2821 gctgatcgaa aagttctcgt gggatcacgt gcagaagtcg gcggcagtct tcaacccgga
     2881 caagttgctc tggatgaatg ccgagtacat caaaaccagt ccaccacgtc aagtagccca
     2941 agcgcttgtg ccgttgctgg agcaagcggg gttgaggaag gaagtcgagg ccgtttcgga
     3001 tgaatggctt gcgcagctgg tggtcctggt gaaggagcgg gccaaaacat tggccgacat
     3061 ggtggagtgg gtcaggcctt attttgggca ggagatccaa tttgaagcgg aggcagcaaa
     3121 gaaatttctc actcccgcgg tcgctccatt gctgcacaag cttctcacgc gcttcgaggc
     3181 gtttcccagc ttttccaaac cggtttggga tgagagtttc aagaagttgg tcgaggaaga
     3241 agacatcaag atgggtgcat tagcccagcc ggtacgcgtc gccttgaccg gacgaacggc
     3301 aagtccggga ctttttgagg tgatggaagt actgggtcgt gaccggacct tgtttcgtct
     3361 ccaaaaaggg cttgagcgcg cctccatggc atgaagagtc ctcctgccaa acccgcgtcg
     3421 atcttgacgg aattgcgatt gatatattac tgtgaacctc ggttggggga tcgtctagcg
     3481 gt
//
LOCUS       DATNDD010000293         2862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2678657, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000293 DATNDD010000000
VERSION     DATNDD010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678657"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..330
                     /locus_tag="VN647_07025"
     tRNA            255..330
                     /locus_tag="VN647_07025"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:288..290,aa:Lys,seq:ttt)
     gene            420..869
                     /locus_tag="VN647_07030"
     CDS             420..869
                     /locus_tag="VN647_07030"
                     /inference="COORDINATES: protein motif:HMM:NF023295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
                     /protein_id="HWG96821.1"
                     /translation="MGLRIGQLMDESLVVNQPVGTKESRDVYRITKSGRTWDLSKVEQ
                     MLQQNVTRTDFAQRLQAIIDAIYNAGSSSADNYFDELVKSAKELRAESERHVREWLTE
                     DELEIFDLLKKDSMTQVEEVLHIHLPESYDRVLFKDKCNHIFEVMVD"
     gene            954..1211
                     /locus_tag="VN647_07035"
     CDS             954..1211
                     /locus_tag="VN647_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HWG96822.1"
                     /translation="MATVTIELPEGAFSALRRSPQEFAKEMRIAAAVPWYAQQQISQE
                     KAAEIAGLSRVEFLNELFRRRVPVSQVMLQELVKEIERAGE"
     gene            1198..1374
                     /locus_tag="VN647_07040"
     CDS             1198..1374
                     /locus_tag="VN647_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96823.1"
                     /translation="MPVSDVWVLNASPVILLGKIGRPDLLESLAAEAVVPNAVFLEIQ
                     AGIGEHQAVQSTAD"
     gene            1399..1590
                     /locus_tag="VN647_07045"
     CDS             1399..1590
                     /locus_tag="VN647_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96824.1"
                     /translation="MPIPLSIERRDLGPGESQVIAHRLKDTQRAVLDDAQGRQCARAH
                     GIAVIGTLGIVLSAPRNNP"
     gene            complement(1766..2008)
                     /locus_tag="VN647_07050"
     CDS             complement(1766..2008)
                     /locus_tag="VN647_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96825.1"
                     /translation="MIKVQSQLVQQTLAAITLVLILGALSMIWPAIMALLLGVLLLLQ
                     SCGVEQEIAVLSWTEVGNMPIVFASLRRSGFPAILS"
BASE COUNT          703 a          719 c          815 g          625 t
ORIGIN      
        1 aagcgaagcg ttacttggct cgcatcgaag agttggcgca gtgccgaatc gatatgatct
       61 cgaccgggtc caaacgtgcg gaaacgatca tgctgcggaa tccattggac tgttctcgcc
      121 ggcgccccaa acgctcctga aaatagtcaa tggccattta ccgggcggga atgctaggat
      181 ggatcttcgt ttgtccgccg cagtgtttga gcgaatgata gaaagttgac gaccaggttc
      241 tttatccctt cagtgagccg gtagctcagt cggtagagca tcgccctttt aaggcgaggg
      301 ccctgggttc gagtcccagc cggctcacca gttttcgcca tactcctgcc caaagtgtga
      361 gtttttcgac cgacatggtg gtcccagggt gatggagttg ctcacaccct gattggtgct
      421 tgggccttcg catcggtcag ttgatggacg aaagcttggt cgttaatcag ccagttggca
      481 ctaaggagag tagagatgtc taccgcatta caaaatcggg gcgtacatgg gatctaagca
      541 aggtcgagca gatgcttcag caaaatgtca cacgcactga tttcgctcaa cgtctacagg
      601 caatcattga tgctatctat aatgctggga gttcttcggc cgacaactac ttcgatgagc
      661 tggtgaaatc cgcgaaagag ctaagggctg agtctgaacg acatgtgcgc gaatggctga
      721 cggaggacga gctggagata tttgaccttt tgaaaaagga ctcgatgact caagttgaag
      781 aagttctgca tatccacctg cctgagagtt atgaccgagt gttgttcaag gataagtgca
      841 atcacatttt tgaagtcatg gttgattaag caagccatgg catgaaatgg gctgtctagg
      901 ttcgccggca gtgggaggga taccgtgaga tcagacagga gatggaggtc atcatggcga
      961 cggtgacaat cgaattaccc gaaggcgcgt tctcagcgtt gcgtcgctcc cctcaggagt
     1021 tcgcgaaaga gatgcgtatc gcggcggccg tcccatggta tgcacagcag caaatttccc
     1081 aagagaaggc ggcggaaatc gccggattga gtcgggtcga attcctgaac gaactgtttc
     1141 gacggcgtgt gccggtctct caagtgatgc tgcaggaatt agtcaaggaa atcgagcgtg
     1201 ccggtgagtg acgtctgggt gctcaatgcg tcgccggtaa ttctattggg gaaaatcggt
     1261 cggccggatc tgctggagag tctggcggcc gaggccgtcg taccaaacgc cgtttttctt
     1321 gaaatccagg ctgggatcgg cgagcaccag gcggtacaga gcacggcgga ttaggctgca
     1381 cctcgccggg tggctgatgt gccgattccc ttgtcgattg aacgacggga tttggggccg
     1441 ggtgagtcac aggtgattgc acatcgcctc aaggacacgc aacgggccgt ccttgacgat
     1501 gcccaaggcc gccaatgcgc acgcgcacat ggaatcgcag tcatcggcac actggggatc
     1561 gtgctgagcg cgccgcgcaa caacccctga agagcgaggc tcacgtgacg agcatatccg
     1621 acagctgata gtcacgcttg tctcgcccat tccgaccgtc tcgtagaatc gagcctccgc
     1681 attggcgctc ataatcacca tcacccccga gctcattccc tcgtggcgga atgctttcgg
     1741 cagtcgaagg caccgctcga actcgtcacg agagaattgc cggaaaaccg gatcgccgaa
     1801 gagaggcgaa gacgatcggc atattgccga cttcggtcca cgacagcacc gcaatctcct
     1861 gttcgacgcc gcaagattgg agcagcagta gtacgcctaa caatagggcc atgatggcgg
     1921 gccagatcat cgacagagca ccgagaatta ggacgagcgt tatcgccgcc aaggtttgct
     1981 ggacgagctg gctttggact ttgatcataa ggtcctccct cgttgatcga atgggttgta
     2041 ccatatgaca taaggaggag taacaatagc taggaaaact actagagcct atctcaaaac
     2101 gccttgcgaa ggccagagag gcttcgctgg gaaatatcgc ggtcacggct ccggacttcg
     2161 cgcaaccgtt ctccgaacag gggcgctttc aattttgaaa tagggtctag taggagttag
     2221 cactgtgatc agtgtttcac agtgatacag atcgacgtct aactgcggcc atcgaagacc
     2281 tcagacatac gagaccaaac gtcgctcgca ggtcttactc aagatcctct gctcacgcac
     2341 tgcgattgtg cagcaaggga atatcctagc ggtgcatgca gtgctgaacg tttacgataa
     2401 aaaatcagag ccccctcaac gcaaggccaa agtacctaag aacaaaaatg tttcggttcg
     2461 tcgaaatcat ttttgagaag aaggcggcca tgatttgtga acaatgccat gtcccgatga
     2521 ttgaaaggct ccatacccga cagagagaag acactgacga agaacaaacg atcatcgtcg
     2581 agtgcctgcg gtgcggccat aacgaatacc agttctttat tccatcgttt tggaggcgat
     2641 tggcggctta gccggatcac atggtttcca acagggaagg agatctcgta atggctgaag
     2701 tcaattccat gtatcatgcg caaactcgga ccgatgcgct caggtcgcag atcgagaagc
     2761 ttgaaggtct tgctcaccat acggtagtga atcctacctt cgagcaattt gataccgaga
     2821 cggaagatct cttgagcacg atctacggcg ccgatcatcg ct
//
LOCUS       DATNDD010000294         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3830916, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000294 DATNDD010000000
VERSION     DATNDD010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3830916"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VN647_07055"
     CDS             <1..291
                     /locus_tag="VN647_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HWG96826.1"
                     /translation="PVSDQTNPVPVAPKKNRGRVDIIRQTAHRGGKTVTVVTGFVGIG
                     QSEKEQLAKQMQKVCGAGGTVKEGRIEIQGDQRDVIAGILTDAGFRPVFAGS"
     gene            425..925
                     /locus_tag="VN647_07060"
     CDS             425..925
                     /locus_tag="VN647_07060"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HWG96827.1"
                     /translation="MFQVTGIGLSFCVLLLILSSCADQSDQVRQPRGYGGMPPGETWV
                     AVDSAYQSPSREMVYYDPKTIRRDGDLVTLWQLIDYKWMQGNAPFGTFMMSPHRFFST
                     KTHKEFDCAHNRVRLLASSEFSQHMGTGTHNAVLVSQGYGQPVEPGSINQALWEVACG
                     VTNTPS"
     gene            922..1413
                     /locus_tag="VN647_07065"
     CDS             922..1413
                     /locus_tag="VN647_07065"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWG96828.1"
                     /translation="MSLTLPVPTHMLNALELKVPPLALVFLFGALMWLVSAYSVFTIA
                     LPWRSALSFIFYTGGSAIVLAGILTFRRMKTTVNPLTPEATTTMVTSGIYRFTRNPMY
                     LGFLLILVGWAIDLSNLLAFAFLPLFVWYMNRFQILPEERALASKFSEASTRYKRSVR
                     RWV"
BASE COUNT          658 a          786 c          748 g          705 t
ORIGIN      
        1 cctgtttctg atcagacaaa tccagtgccc gtcgcgccca agaagaatcg tggccgggtg
       61 gatatcatcc gtcagacagc gcatcgcggc ggcaagacgg tcacggtggt cacagggttt
      121 gtcgggatcg gacagtctga gaaagagcag ctggccaagc agatgcagaa ggtctgtgga
      181 gcgggaggta cggtcaaaga gggacggatc gagattcaag gagatcagcg agacgtaatc
      241 gctggcattc tcaccgacgc cggatttcgt ccggtgtttg ccggaagcta agttcccgac
      301 gtaagcagag gtcagctcag cctattccct ttgtctctca caagctgctt acagacacat
      361 agtgaacagc ccttgcacct tccttttcac cctttgctag aatgcgttat ccggtgaggg
      421 atctatgttc caggttaccg gtatcggcct ttctttctgt gtcctccttc tcatcctgtc
      481 gtcctgtgca gaccagagtg atcaggtccg tcaaccccgg ggatatggcg ggatgccgcc
      541 tggggaaacc tgggtagccg tcgatagcgc gtaccaatct ccatcgcggg aaatggtgta
      601 ctacgatccc aagacgattc gccgagacgg agatctcgtg acgctgtggc agttaataga
      661 ctataagtgg atgcaaggga atgcgccgtt tgggacattc atgatgagcc ctcaccggtt
      721 tttctcgacg aagacgcaca aggaatttga ctgcgcacac aatcgtgttc gactactggc
      781 gtcttcggaa ttctctcagc atatgggcac aggaactcac aatgcggtat tggtcagcca
      841 aggttatggg caaccagtgg aaccaggcag tatcaaccag gcactttggg aagtggcctg
      901 tggggtgacc aacaccccca gttgagcctg accttacccg tcccgacgca catgttgaat
      961 gcactggaac taaaagtacc gccgcttgcg ctcgtattct tgttcggcgc gctgatgtgg
     1021 ctcgtctctg cctattctgt attcaccatc gccttgccat ggcgttcggc gctctccttt
     1081 attttttaca cgggaggatc agcgatcgtg ctggccggca tcttgacgtt tcgccggatg
     1141 aaaactaccg ttaatccact cacgccggaa gcgaccacga caatggtcac ctctggcatt
     1201 taccgcttca cccgtaaccc gatgtacctg ggcttcctct tgattcttgt tggttgggcg
     1261 attgatctct ccaatctact ggccttcgca ttcctaccgc tcttcgtgtg gtatatgaat
     1321 cgttttcaaa tcctacccga ggaacgagcc ttggcctcca aattctcaga ggcatctaca
     1381 cggtacaagc gttcagttcg ccgatgggta tgatggctca atgctcaacc ttcctgcgaa
     1441 ccagcgcgcc tgaaaaacga tagcgagaag atgctaatgg ttcggcaagg tattggtact
     1501 gtaacccccc gatccgtagt ccggtgctct atccattgag tatggagcaa agattgaatg
     1561 ggcaattcag atagtagggc tgtcggtagc gcactcgctt tgggcgcgag gccagtggac
     1621 ttcctcatta caccgaaaag ggtcaggcct tccatcaggc tccggtcata gtcatagcaa
     1681 atgaggaaat gaggtgggcg ctatatcgct ttcgcatgtc ttcgggaacc gcaccaacgc
     1741 agccgctatt tccattgcac ccaagttcca gcctaccatc tcgcttgacg gaatagttcc
     1801 ttaccacgta gcatatacct atgacatttc agcacgccga ctgtctcatc cctcgcgtgt
     1861 tcgtccattc ttgacgcgat cgatttccca gtctccgcgt tccaacgccg agtgaccctc
     1921 cgcgcttcac caaacgcagc gatcacgaag aggagtcgcc gatgcttatc aacagaccga
     1981 tcaccgacgg aattctggcc gtgctccaaa actctacaga gtgtgacttt gagctcttag
     2041 tggctcgata ccctgagcac acctggaacg aactttttca ggaggtgggt agattgagcc
     2101 ggactgggca agtgatcatt acaagaggag ttggagtgtt cacggtcaga ttggcgcctg
     2161 tcaaataaac accggctata aaagggcttg gggtcatttt ctttgacagc ctttatgttt
     2221 gaagggatcg ggagtctttt ctgacgaacc atttcccggc aagatcagga tacccgcaca
     2281 ggaagagaca aacaagcgat gtgaattcaa ggttggcgag aagaaaacgg taccaggccc
     2341 catttccggt cgcgtggcgg cgggctcacc ataggccatg aggtggaaga tcggcgagcg
     2401 catctcactc tcaaattgaa acgtttcacg agatgcgctc ccgagacatg acagaactct
     2461 accggtctac tctgcacagt tacgttcctt tcttatagac ccatatgcca gcgcccacta
     2521 gcccaatcag tgctattact aatgttaaga ggttaacgtc catggtggct cctttctgca
     2581 ttttactgcc ttccataaac catagacgct tcccgatggc ttagcgagct ttataacgtg
     2641 cctggcctcg ccggcaccgc gtttccatcc tctcttatga tttcaataac ttgaccaaat
     2701 cgagaactga acctcaagac acaggccaat gttgaaggat cattatccat cagttactag
     2761 ggaaggtctg attaattcac gttttcactc ggaaatctgt ttgtggggtg gccaccaggg
     2821 ggaatccgct ggttctggag ccttttcgtg gggtacaccc actgtccact ggcccactgc
     2881 agctctgtgg acacacg
//
LOCUS       DATNDD010000295         7449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2724025, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000295 DATNDD010000000
VERSION     DATNDD010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724025"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            728..925
                     /locus_tag="VN647_07070"
     CDS             728..925
                     /locus_tag="VN647_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96829.1"
                     /translation="MAAGAGMAHCEPNEKNDHLRSRQFEEQVQDQVPARHLHVHVVLW
                     DESPDDAAEEDDETVDEMSRP"
     gene            1048..2088
                     /locus_tag="VN647_07075"
     CDS             1048..2088
                     /locus_tag="VN647_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96830.1"
                     /translation="MKKVMITTAAVALAFGLSPLAGAQDQDVRNHSTGTQDNRVSDSL
                     NDNSDNRKTITKNDNDTKTVTVDKTITKTNTDSKNSGQASAGDYGAAANNHSTATTNL
                     HVDLNNAFNKTHIVNATKLDGYVSHNNVHDIGNRGGDAYSFSPGGYGGDGYAKAKAYS
                     GDAYGGDGGKGGKARSGDGGYAYGGDGGYAKTGHGGAATGGSTTSNGNNGGQSNGGDN
                     RHNRTHGATDWDAGPGGQAQGGNSGNANGGNGGYAQGERSGDANGGHGGDGASGESGY
                     AAAQNQNSGNGGNGGRGGDTYGGSAGTFNASNTLSGLSGAAGVTVAIQNTGIAAMQQV
                     GVTTMANVTVGH"
     gene            2158..2517
                     /locus_tag="VN647_07080"
     CDS             2158..2517
                     /locus_tag="VN647_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96831.1"
                     /translation="MRLYFSIVVSVLGLCISTAHADSSETRSVSSTAITSWGSPVPAE
                     QLDTQRGGTDTGPVTINANILNAKLYDNAAVDNVTGHNSINGNAFTGASGLPIVFQNS
                     GNNVIMQSGTVLNLTLK"
     gene            2523..3215
                     /locus_tag="VN647_07085"
     CDS             2523..3215
                     /locus_tag="VN647_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HWG96832.1"
                     /translation="MATKQIIGYVVAMALVASTGAMAATVEVTPGVGSRMLLQVWSMR
                     ELKVRSVILQKYDYSCGSAAVATLLTYHYDHPLTEETAFRMMFDHGNQDKIQKEGFSF
                     LDMKRFLDAHGYPADGFQVSLDDLSKAGIPAIVLMVDKGYHHFVVVKGMRGDKVLLGD
                     PAVGLRVVPREQFEASWPSRVVFVIHDFPARGEFNTARDWSVRPMSPLGRPLSPDTLN
                     NIVLFRPGGRDF"
     gene            3215..3778
                     /locus_tag="VN647_07090"
     CDS             3215..3778
                     /locus_tag="VN647_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96833.1"
                     /translation="MNRLLKSIRRMVKIGILTLLGAQSMTPAMASMKDEYFARLTPLS
                     EAVLDSMRGGFQRGPDGPFMSFGIERNVFINEKLINSMTFNIPDLKQLTDQRDMRPSV
                     DHSGMKPFADHPNETFTLIQSGPGNSFHPDLSTLPPFMTVIQNSLDNRTIQSHTVINA
                     TVEALTLARSLDLGNALSQANMEAIRH"
     gene            3965..5167
                     /locus_tag="VN647_07095"
     CDS             3965..5167
                     /locus_tag="VN647_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96834.1"
                     /translation="MSLVPSQGYTAQLGFIDRHVEQRDTDDRKHLHHTSSQSPRDASQ
                     YLAANEQTAQAAEPRRREQNQPVPEEGPAEGPKPTGPVGVAPASPQEILPSWMVRTFD
                     LPGAGVLTPKGTLIVEPYLQFAHLSNSRVTLTGFTIVPALTIGLINIQGVSRDIYTAA
                     LVGRYGITNRLEVEVRVPSLYREDSITQRPIATPSQTDTISTFNGSGLGDVETTGRFQ
                     INQGGPDKPYFIGFARFKSRSGTGPFDVPRDPVTGVQTELPTGSGFYIFQPGLTMLFP
                     SDPVVLFSSVSFIYNIPRDVGGQIGHIDPGSGGNANVGLGVSLNEKLSLTLGYDHTVF
                     SRPTSASNILLTTTPHVTQIGVLLIGGSYRWSDRSFVNFVVGIGATREAPDLQVTLRI
                     PTAFPLWK"
     gene            complement(5301..5867)
                     /locus_tag="VN647_07100"
     CDS             complement(5301..5867)
                     /locus_tag="VN647_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWG96835.1"
                     /translation="MASAFSPCRCRRCTRQSVSTSHHDMACLIVRCSLLYCAGSGCDR
                     LLLRLHYGDLWGHRGMTHSLLFAGLLSAVLVGMWSKQKTRAAQVGIFVYLFFCTASHG
                     VLDALTDGGLGVAFFSPFDTSRYFFAARPVAVSPIGISTFFSEDAVHVLASEVTWIWL
                     PTIAGFFIVRGLQYARSAQSASKQAPQD"
     gene            complement(5899..6360)
                     /locus_tag="VN647_07105"
     CDS             complement(5899..6360)
                     /locus_tag="VN647_07105"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HWG96836.1"
                     /translation="MFDVILYQPEIPPNTGNIIRLCANTGVRLHLVKPLGFSLEDKQL
                     TRAGLDYREFATISAYDNWAACEERFADSRLFAVSTKGTHRYDQIAYAKNDVFLFGPE
                     SRGLPTELLEAVSEQQRIRLPMTPGSRSLNLSNTVAVVVYEAWRQIGFDIG"
     gene            complement(6461..>7449)
                     /locus_tag="VN647_07110"
     CDS             complement(6461..>7449)
                     /locus_tag="VN647_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96837.1"
                     /translation="IADTLHLTHRVGYPQGVVTDRWDEGVCRLTQQTDGMVNLWQVAN
                     SISQDQHIENLPLSLWGVGGEIASGTYHRNLNLPPTLGGSQMLRTFLSCLVAKDTELL
                     KQETRQLALQSVRETCEGFLDDGFEPFVVPDAFYIFDRVRRWAGTNTRKASPVCDMFT
                     PFCTIPYIEAAHSMPLHDRIAGRLHTELIRATTPSLLDFPYDKPRRFPLPKLGVKRLV
                     AQTVREAAPRWFLRVLRYGASQLQRRKLEGKTGPVQAEWVEAKRLAIQDVCLNQRSSV
                     LWDYVDRRSFEDIMSSKTSGKVRRQHCAKILSIATLFYYEAEQLDRSGLEKR"
BASE COUNT         1835 a         1919 c         2055 g         1640 t
ORIGIN      
        1 tcgatggcgc gaggccatcg gaatggcacc taatgtgacg agcgaatccc aagggatgat
       61 gtgaacgggc gtgctgcgtt atagatcagt ccaaccggaa cgtggtgtag agacgctgtt
      121 cattctgaca cgcgtctttc caaagaaatc tcttcaaggc tcgcataccg acctttattc
      181 acccacgcag ttcgtgtgcc gaagattgtg agagctgtgg agcgactcgc gccgtagtca
      241 cgagggccgt ttggatccgt ccatttgtag gacagttcaa atgggccccc cggtgatcct
      301 tctgcaggta agaatacttg cgtacggtac aaaatacaca aatcaatcaa ttggttcgcc
      361 tacattcatc taagcccgtg gaagccggga gtctactcgt ttgagctgaa atagactctc
      421 agatgactgt aacgcaatag aacaatcgac cccatcctct ctgtaatcgg ccctattcat
      481 ttcactaggc cttatgaaat caagcgctta cacctaggca tttggattgc tcatgttgac
      541 tcaacaacgt tatgggcggt gtgcagcgat cagggcacac ccgggggaag tcgttaaaaa
      601 tgtacgaggg aattaattct aaattataag cactaagtgc aattatgttt gccatgagag
      661 aagcggccta cgttcacaga attcagggct catgggggtt cggcgagttg cggttcggca
      721 gccacgcatg gctgcgggtg ctggcatggc gcattgtgaa ccgaatgaga agaacgatca
      781 cttacgcagc cgtcaattcg aagagcaagt ccaagatcag gttcctgctc gccatttgca
      841 cgtccatgtg gtgctgtggg acgaatcgcc ggatgacgcc gctgaggaag atgacgaaac
      901 ggtcgatgag atgagtagac cttaaggacg agagaacagg aacaggaaag aggcatgtga
      961 agggaggtga acagcaatcg ctgtgcgaat gtcaggcagc gtggttcggc agttttttct
     1021 ttaaccgttc attctgagga ggatgcaatg aagaaagtga tgatcacgac ggcagcagtg
     1081 gcattggcct tcggtctgtc gcctttagcg ggagctcaag atcaagacgt ccgcaatcac
     1141 tcgacaggca cgcaagataa tcgagtgagc gatagcttga atgacaatag tgacaatagg
     1201 aagaccatta cgaagaacga taacgacacc aaaaccgtga cggtcgacaa gaccattact
     1261 aagactaata ccgactccaa gaattctgga caagcatcag ccggggacta tggagcggcg
     1321 gccaacaacc attcgaccgc taccaccaac ttgcatgtgg atctgaacaa cgcgttcaac
     1381 aagactcata tcgtcaatgc caccaagctt gatggatacg tctcacataa caacgtccac
     1441 gacatcggca ataggggcgg cgacgcctat tccttcagcc ccgggggcta tggtggagat
     1501 ggttacgcca aggccaaagc atattctggg gatgcttatg gtggcgacgg cgggaaaggt
     1561 ggcaaggcca ggagcggcga tggcggctat gcctacggcg gcgatggtgg ttatgccaag
     1621 accggacatg gtggcgctgc taccggtggc agtactactt ccaacggcaa caatggtggt
     1681 caatccaacg gcggagacaa tcgtcacaat aggacacacg gcgctaccga ttgggacgct
     1741 ggacctggcg gtcaagcaca gggtggaaac agcggtaatg ccaacggtgg caatggtgga
     1801 tacgcccaag gcgagcgtag cggagatgca aacggtggcc atggcggaga cggtgcctcc
     1861 ggggagagcg gctacgcggc tgcgcagaac caaaatagtg gaaacggtgg aaacggcggt
     1921 cgaggagggg atacgtacgg cggaagcgcc gggactttca atgcctcgaa tacgctttcc
     1981 gggttgagcg gcgctgccgg cgtgaccgtg gctatccaga acacggggat cgcggccatg
     2041 caacaggtcg gagtcaccac gatggcgaac gtcacggttg gtcactaagc agaatccggc
     2101 tggaggagca ccggctcctc cggctgcact ccacgactta tgaactgaga gggcatcatg
     2161 cgtctttact tttcaatagt ggtgagtgtg cttgggctct gcataagcac ggctcacgcc
     2221 gactcctcgg aaacgaggtc ggtctcatcg acggcgatca catcgtgggg cagccccgtc
     2281 ccggctgagc agttagatac tcaacggggg ggaacggata ccggtccggt cacaatcaat
     2341 gccaacatat tgaacgccaa gctctacgac aatgccgccg tcgacaatgt gaccggtcat
     2401 aattcgatca atggtaatgc gtttacggga gccagtggtc tgccgatcgt tttccaaaat
     2461 tccggcaaca acgtaattat gcagagtggc accgttctga atctgacatt gaagtgatgg
     2521 cgatggcgac aaagcagatt attggatatg tggtcgccat ggcattggtt gcttcgaccg
     2581 gcgcgatggc ggccacagtt gaagtgactc cgggcgtggg gagtcgcatg ttgctccaag
     2641 tctggagtat gcgcgaactg aaggtccggt ctgtcatcct tcagaaatat gactatagtt
     2701 gtggctcggc cgccgtcgcc accctcttaa cgtatcatta cgatcatccg cttactgaag
     2761 agacggcgtt tcgcatgatg ttcgaccacg gtaaccagga caaaatccag aaggaagggt
     2821 tttcgttcct ggacatgaag cgattcttag atgcccatgg gtatccggcc gacggctttc
     2881 aagtctcgtt ggatgatttg tcgaaggccg gtattccggc gattgttctg atggtcgata
     2941 aggggtatca ccatttcgtg gtggtgaaag gcatgcgggg cgataaggtc ctgctcggtg
     3001 acccggcagt cggattacgt gtcgtgccgc gcgagcagtt tgaggcctca tggcccagcc
     3061 gggtggtgtt tgtcattcat gactttccgg cgcgtggaga atttaataca gcccgtgatt
     3121 ggagcgtccg tccgatgtcc ccactcggac gaccgttgag ccctgacaca ttaaacaaca
     3181 tcgtcctgtt tcgcccagga gggagggatt tctgatgaat cgcttattga aatcgattcg
     3241 ccggatggta aagatcggca tactgactct cctgggtgct cagtctatga ctcccgccat
     3301 ggcctccatg aaggatgaat attttgcccg tctgacaccg ctgagtgaag cagtcttgga
     3361 cagcatgcga gggggatttc aacgaggccc agatggtccc ttcatgtcat tcgggattga
     3421 acggaacgtg ttcatcaatg aaaagctcat taattcgatg acgtttaata tcccggactt
     3481 gaaacaattg accgatcagc gtgacatgag accatctgtc gatcattcag gcatgaaacc
     3541 tttcgccgat catccgaatg agaccttcac cctcatccaa agcggaccgg gcaattcgtt
     3601 ccatcccgat ctgtctaccc tgccgccgtt tatgaccgtc attcaaaact cgcttgataa
     3661 ccgaaccatt cagagtcata ccgtcatcaa cgccacagtg gaggccctga ccttggctcg
     3721 atcactcgac ctagggaatg cgctctcaca ggccaacatg gaggcaatcc gacattgaaa
     3781 atggctcagc aagccgaatc ccataaaagg gatggccaat gatagtcaga gtatcttcaa
     3841 gagatcgaag aaacgatgga ggctgcattt gtaccgatct gaacggaggc catcgatcga
     3901 acttttgaag actgccaaaa aaacttgtga agctggttgc gtaatttttg ggctaggttt
     3961 tatcatgagc ctggtgccct ctcaaggcta tacggctcag ctcggattca tcgatcgaca
     4021 tgtcgagcag cgagatacgg acgacaggaa gcatcttcac cacacgagct ctcagtcgcc
     4081 acgggacgct tcgcaatacc tcgccgcaaa tgaacagacc gctcaggccg ccgaaccgag
     4141 gcgtcgcgag cagaatcaac ccgttccgga agagggtcct gcggaaggtc caaaacctac
     4201 cggtccggtg ggggtcgctc cggcaagtcc acaggaaatt cttccttcat ggatggttcg
     4261 gacattcgat ttacctggag ccggggtgct gacgcccaag ggcacgctca tagtagagcc
     4321 atatctgcaa tttgcccatc tgagtaatag tcgggtgaca ctcactggat tcaccatcgt
     4381 tccagccctt acgatcggtc tcatcaatat ccagggtgtg agccgggaca tttatactgc
     4441 ggcacttgtc gggcgttacg gaataacgaa tcggttggaa gtcgaggtga gagtgccctc
     4501 tctttatcgg gaagattcga ttacacagcg acctatcgcc actccatcgc agactgatac
     4561 catctcgacc ttcaacgggt cgggtctcgg agatgtcgaa accacaggcc gatttcagat
     4621 caaccaaggt gggcccgaca aaccatactt catcgggttc gctcgattca agagccgcag
     4681 cggaacaggt ccgtttgacg tgccgagaga cccggttaca ggggtacaga cagaattacc
     4741 aacgggatcc ggattttaca ttttccaacc tggcctgacc atgcttttcc ccagcgaccc
     4801 ggttgtcctc ttcagcagcg tgagcttcat ctataatatc cctcgcgatg tcggcggcca
     4861 aatcgggcac atcgatcccg gaagcggcgg caatgccaat gtggggctcg gtgtttcgct
     4921 gaatgaaaag ttgtcgttga cgttagggta tgaccatacc gtcttttcaa ggcccacgtc
     4981 cgcctccaat attctcctca ccaccacgcc gcatgtcacc caaattggag tcttgctcat
     5041 tggaggctcg tatcgatgga gtgatcgcag ctttgtgaac tttgtcgtcg gcatcggagc
     5101 gacacgagag gcgccggacc ttcaagtgac gctccggatc ccaacggcct tcccgttatg
     5161 gaaataggta gatccgtcag acgcgatagc ccgatgcagg gtaagatcaa tcggtcattt
     5221 tagcgtcagt cttgttacgt acatcatttg ttcctgagaa ttcacgatgc gactgatggt
     5281 cacactgtta ccgagagtga tcaatcttgc ggtgcttgct tcgaagccga ttgagccgat
     5341 cgtgcatatt gtagtccgcg aacgatgaag aaccccgcaa tggttgggag ccagatccat
     5401 gtcacctcac tggcaaggac gtgaacggca tcctcgctaa aaaatgtgct gatgccgatc
     5461 ggggacacgg ccaccggccg agccgcgaaa aaatatcggc ttgtatcgaa cggagagaag
     5521 aaggccacac cgagtccgcc gtcggtcagc gcatcgagca caccatgtga tgcggtgcag
     5581 aaaaagagat aaacaaatat tcccacctgc gctgcccttg ttttctgctt ggaccacatg
     5641 cccacgagaa cagcgctcaa caggccggca aacaagagag aatgcgtcat gccgcgatga
     5701 ccccagagat cgccgtaatg gagccgaagt agaagccgat cacatccaga tccggcacaa
     5761 tagagcagac tgcacctgac aataagacag gccatgtcat gatgggatgt tgaaacgctt
     5821 tgcctagtgc aacggcgaca acggcatggg gagaacgcag aggccatact tggttttcgt
     5881 aaggctgcgg gcaggctctc agccgatgtc gaatccgatt tgcctccaag cctcgtacac
     5941 cacgactgcg acagtattgg aaagattcaa acttctgctc ccgggcgtca taggaaggcg
     6001 aatccgttgt tgttcggaca ctgcctccaa caattcggtc ggcagtcctc ggctttccgg
     6061 tccgaacaga aatacatcgt tctttgcgta tgcaatctga tcgtaacggt gagtgccttt
     6121 cgtagagacg gcaaatagac gactgtctgc aaaacgttct tcacaagccg cccagttatc
     6181 gtaggcgctg atggttgcaa attcgcggta atccaatccg gcgcgtgtca gctgcttatc
     6241 ttccagcgag aaccccaacg gtttgacaag gtgtaggcgc acaccggtgt tggcgcagag
     6301 gcggatgata ttgccggtat tcgggggaat ttccggctga taaaggatga catcgaacat
     6361 agagagggaa aatgtatcat gtggaacgtg ctcaaatgag cacaatagac gcaagcctga
     6421 tttgggtcgg gtttgaatgg tcctcaaacc taatagagtt ctatcttttc tccagtcccg
     6481 accggtctag ctgttctgcc tcgtagtaaa acagtgtagc aatggacaaa atctttgcac
     6541 agtgttgccg gcgaactttt cccgaagttt tggacgacat gatatcttca aatgatcggc
     6601 ggtcgacata gtcccacaat acggaactac gttggttgag acagacatcc tggatcgcca
     6661 agcgttttgc ttccacccac tcagcctgca caggaccggt tttcccttct aacttcctcc
     6721 gttgtaactg gctagcaccg taccgcaaaa ccctcaggaa ccatctcgga gccgcttctc
     6781 taacagtctg ggccaccaac cgcttaacac ctagtttcgg gagcgggaaa cgccgtggct
     6841 tgtcgtaggg aaagtcgaga agtgatggtg ttgtagctcg aataagctcg gtgtgtagcc
     6901 gtccggcgat gcggtcatgc agtggcattg aatgagcggc ctcgatatac ggaattgtgc
     6961 aaaacggtgt gaacatgtca caaaccggag aagccttacg tgtattggta cccgcccagc
     7021 gtcgaacgcg atcaaatata tagaacgcgt cgggcacaac aaatggttcg aatccatcat
     7081 ccaagaagcc ttcgcaagtt tcgcgcacgc tttggagagc gagttgtcga gtttcctgtt
     7141 tgagtagttc agtatctttc gctacgagac aagaaaggaa cgtcctcagc atctggctac
     7201 cgccgagtgt cggaggcaaa ttcaagttac gatggtaggt tccgctagcg atctcacccc
     7261 ctacccccca aagcgacaac ggtaggtttt caatatgttg gtcctgcgag atagaattag
     7321 caacctgcca cagattgacc atgccgtcgg tctgttgagt cagtcggcat accccttcgt
     7381 cccaacgatc cgtaaccact ccctgtgggt agccaacccg atgagtcaag tgtaaggtgt
     7441 cggcaattt
//
LOCUS       DATNDD010000296         2436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2557310, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000296 DATNDD010000000
VERSION     DATNDD010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557310"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VN647_07115"
     CDS             complement(<1..452)
                     /locus_tag="VN647_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96838.1"
                     /translation="MDTPSNISPPSPLFDAIGRMAFRHFPQSGTFNAHRMRDYGIAAF
                     TFFCVAVSSLLEISASVERQQLLGAFAWMFLGGLLLGENRETKAQVVIAVLFATIGEH
                     FASIYMGGYTYRFENVPAYVPPGHGMVYLTAVALARSGLFIRHPGKIAL"
     gene            complement(879..2138)
                     /locus_tag="VN647_07120"
     CDS             complement(879..2138)
                     /locus_tag="VN647_07120"
                     /inference="COORDINATES: protein motif:HMM:NF041640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HWG96839.1"
                     /translation="MNAKHVFKLWVMGLCGVATLAFTPVLDITPAFAHGERSQEPFLR
                     MRTVNWYDTEWVGKSTKVNDVTELRGKFHLSEDWPRAVVKPNRTFLNIGSPSSVFVRL
                     SSKINGTPMFVAGPMEIGRDYEYVVVLKARLPGHHHIHPMFAVKDAGPIAGPGGWMDI
                     TGRYEDFTNPIKTLTGETFDSETMGTATGIMWHLFWAAVAIFWVGFFMIRPMYLVRAR
                     VLAAYGDEILLDPIDRKVGTAMLIFVLVVVTVGYLAADARHPVSVPLQAGETKVKPMP
                     IKPNPFVVEVTHAEYDVPGRALRMTLHATNNGTEPVTIGEFTTAGIRFTNKMGATKLD
                     PNYPAELVAQAGLTMDNEAAIQPGQTVDIKIESKDVLWEVQRLVDILHDPDQRFAGLL
                     MSWTDKGERLINPIWAPVLPVFTRLGA"
     gene            complement(2135..>2436)
                     /locus_tag="VN647_07125"
     CDS             complement(2135..>2436)
                     /locus_tag="VN647_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit A"
                     /protein_id="HWG96840.1"
                     /translation="LSWADYMGFAYVRTGTPEYIRMIEVGSLRTFGGHSTMISSFFAA
                     FASSLMYILWWQFGKFFCTSYFYLTDDRQKTTKVYDVFAYATLANQDKAKIGGKA"
BASE COUNT          594 a          667 c          676 g          499 t
ORIGIN      
        1 agcgctattt tcccgggatg acggataaac aacccggatc gtgcaagcgc cacggccgtc
       61 agatacacca tcccatgacc gggcggcaca taggcgggaa cattttcgaa ccggtatgtg
      121 tatcctccca tgtaaataga cgcgaaatgt tcgccgatgg ttgcaaatag gacagcaatg
      181 acgacctgag ccttagtttc ccgattctcg ccaagcaaca agccaccgag gaacatccat
      241 gcaaacgcac caagcaattg ttgccgctcg acgcttgcac tgatctccaa caaggaactg
      301 accgccacgc agaaaaaggt gaaggcggca attccgtagt cccgcattcg atgagcgttg
      361 aacgtaccag actgagggaa atgtcggaac gccatgcgcc cgatggcgtc gaataacggt
      421 gaagggggag aaatgtttga aggggtatcc atggatcgag cagaaggtat gacgcaggga
      481 tcctgtgtgt tgaaccctcc gtaggaacaa caggatttgc aatccaccag tctcgtaaga
      541 gcggtaacat catacaggac ccttgtcaga cgggcaagtt gtggcaagac gacgagccta
      601 ttcctcagga tttgtaaaga tgagatcaac cgaggctcag agcagattcg ttgcgcacaa
      661 ctagtgggac actggtgacg aagatgcggt acgtatagct aggacacact cgaacggtta
      721 ctgtccactg gcgctaggct gttccatgag cgatgcacgg ggaggagaca ggcatgcaca
      781 gggtgaaagc agagaatcgg ggaggaaaaa acaaaaacgc cgacccgcct cgccatttcc
      841 atgggggagg gccggcgtct tgttagagga gcgttgctct aggcacccag tcttgtgaag
      901 accgggagaa ccggagccca gattggattg atgagccgct cacccttgtc tgtccaggac
      961 atcaacaatc ctgcgaagcg ctgatccgga tcgtggagaa tgtccaccaa ccgctgcact
     1021 tcccacagaa cgtctttaga ctcgatcttg atgtctacag tctgaccagg ctggatcgcg
     1081 gcttcattgt ccatcgtcaa gccagcttgt gcaaccagct cggccggata gtttggatcg
     1141 agtttggttg cccccatctt gttggtaaac cggatgcctg cagtcgtgaa ttcaccgatg
     1201 gtcacaggct cggtgccgtt attggtcgca tgaagagtca tgcgcaaggc ccgacctgga
     1261 acgtcgtatt cagcatgggt gacttcaacc acgaacgggt ttggcttgat cggcatcggc
     1321 ttgacctttg tttcaccggc ctgaagcggt acgcttacag gatggcgcgc atccgccgcc
     1381 agatacccga cggtgacgac gaccaacaca aagatcagca tcgcggttcc gactttgcgg
     1441 tcgatagggt cgagcaagat ctcgtcccca tatgccgcca acacccgtgc acggaccagg
     1501 tacatcggtc ggatcatgaa gaagccgacc cagaagatgg caactgccgc ccagaacagg
     1561 tgccacataa taccggtcgc agtacccatc gtttctgagt cgaacgtttc accggttagt
     1621 gtcttgatcg gattggtgaa gtcctcatat cgaccagtga tgtccatcca tccaccgggt
     1681 cctgcgatcg gacccgcatc cttgacggca aacattggat ggatgtgatg atgaccgggg
     1741 agtcgagcct tcaagacgac cacatactcg taatcccgtc caatttccat cggaccagcg
     1801 acgaacatcg gcgtcccgtt tatttttgag ctaagacgca cgaacactga actgggggaa
     1861 ccgatgttga ggaaggtacg gtttggtttc actaccgcac gaggccaatc ctcggataaa
     1921 tgaaacttgc ccctcagctc ggtcacatcg ttgaccttgg tcgactttcc tacccattct
     1981 gtatcgtacc aattcacggt gcgcatccgc aggaacggtt cctgcgaacg ttcgccgtga
     2041 gcgaatgcag gggtgatatc gagaaccggc gtgaacgcca gcgtcgccac cccgcagagg
     2101 cccattaccc agagtttaaa gacgtgtttg gcgttcatgc tttccccccg attttggctt
     2161 tgtcctggtt cgccagggtt gcgtaggcga acacatcgta caccttcgtg gtcttctgtc
     2221 ggtcatccgt gaggtagaag taggaggtac agaagaactt cccgaactgc caccacagga
     2281 tgtacatcaa cgacgaggcg aaggcggcga agaacgagga aatcattgtg ctgtgcccgc
     2341 cgaaggtccg cagcgagccc acttcgatca tccgaatata ctccggcgtc ccggtccgga
     2401 catacgcaaa gcccatgtag tccgcccagg agagaa
//
LOCUS       DATNDD010000297         6150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1197183, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000297 DATNDD010000000
VERSION     DATNDD010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197183"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VN647_07130"
     CDS             <1..398
                     /locus_tag="VN647_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HWG96841.1"
                     /translation="KRDEKATEVVSKIHKPGKVKPDPLRGLFEIKLDCKPAVVEYEPD
                     SELRDTEQIPLLEEGGIEAFIRREVLPHVLDAWYDPESVKTGYEISFTRYFYKPQPLR
                     SLEEIRADILALERETEGLLGEIIGEVTQ"
     gene            395..1753
                     /locus_tag="VN647_07135"
     CDS             395..1753
                     /locus_tag="VN647_07135"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWG96842.1"
                     /translation="MIADLKPYSAYKDSGVPWLGKVPSTWSLARNGGLFVQRNETGFV
                     HLPILEVSLKTGVRVRNFDESGRKQVMSDRDKYKRARKGDLAYNMMRMWQGAIGMAPT
                     DGLVSPAYVVTRPLRGVEPKFYLNLFRIDAYMGEVDKFSHGIVKDRNRLYWEDFKQMP
                     SPVPPTDEQAAIVRFLDHADRRIKRYIRAKQKLIKLLEEQKQAIIHRAVTRGLDPNVR
                     LKPSGVEWLGDVPEHWEVVRLKTLCSMKSGDSITAMSIEPVGKYPVYGGNGIRGYTST
                     YTHDGDFALVGRQGALCGNVHLASGRFWASEHAVVTTLRPSNDLKWFAALLRVMNLSQ
                     YSIAAAQPGLAVERILNLSVPAPPMSEQSRIAVHMDMTAVGLVAAIQRSGREIELLRE
                     YRTRLIADVVTGKLDVREAAAKLPDEPDEPESFDEVDVPVDDDEKAEGTDLDAVADEV
                     EA"
     gene            1750..2400
                     /locus_tag="VN647_07140"
     CDS             1750..2400
                     /locus_tag="VN647_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HWG96843.1"
                     /translation="MSPVPAEPKIYHITHVDNLAAIVNDGVLLSDAAIVRRSGPSVTI
                     GMSEIKRRRIEEIPVSCHPGTRIGDYVPFYFCPRSVMLFLIHCANHPDLTYRGGQGPV
                     VHLEADMHEVVSWAEQGARKWAFSLSNAGAYYVQVRGRLDELEEINWAAVSATDFRSP
                     DVKEGKQAEFLVHGSFPWELVRRVGVCSARIKAQAEAALQIAAHRPPVEILRDWYY"
     gene            2416..3480
                     /locus_tag="VN647_07145"
     CDS             2416..3480
                     /locus_tag="VN647_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HWG96844.1"
                     /translation="MIEPTTGNILAADAEALVNTVNCVGIMGRGIALQFRKAFPDNFK
                     AYEAVCKRGELQPGHMFIFDTGSLTNPRYIINFPTKRHWKGKSRLADIELGLTALVSD
                     IHRLGIRSIAIPPLGCGQGGLNWADVRPRIERALAAVPDVGVLLFEPTGNSVHSASTP
                     SRSIPKMTEARAALVGLMQRYLAGLMDPFISLLEVHKLLYFMQEAGESSLRLRYVKAP
                     YGPYAENLRHLLLSVEGYLTSGYTDGVDDPEKPFELVPGAIADAEHFLEGHPHTQHRF
                     QRVADLVKGFETPFGLELLSTVHWVSTREGALTTDEALAATFRWNERKRRFSPQQIAL
                     AWNVLRDKGWVSAQPAAHHA"
     gene            3477..>6150
                     /locus_tag="VN647_07150"
     CDS             3477..>6150
                     /locus_tag="VN647_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HWG96845.1"
                     /translation="MKTDTSEKGLENLIVAAMAGQASDEGPPSGEGISLVRERYGGTG
                     WIPGQAEDYDREFCIDLPQLRAFLHTTQPKVAEAFDLNNDSPIRRKLLARLQGEISKR
                     GVIDVLRHGIKDGPHHVDLFYGTPSPGNEKAATLNASNRFTVTRQLRYSRDETQQALD
                     LALFINGLPIATFELKNSLTKQTVEDAVEQYKRDRDSREKLFEFGRCVVHFALDDHEV
                     RFCTHLKGKSSWFLPFNLGWNDGAGNPPNPYGIKTDYLWHKILTRDGMTDILENYAQI
                     VEEKNEKTGKKKAVQIWPRFHQLDVVRKLLSDTRQQGGGLRYLIQHSAGSGKSNSIAW
                     LAHQLIGLQKDDALVFDSIIVVTDRRILDKQIRDSIKQFAQVGATVGHAEHSGDLRKF
                     IAEGKKIIISTVQKFPFILDEIGSEQRGRKFAIIIDEAHSSQGGRTSAALSMALSTAG
                     AEEDDETTEDKINRIMEAKKLLPNASYFAFTATPKNKTLEIFGDALPPDAEGKVRHQP
                     FHSYTMKQAIQEGFILDVLKHYTQVESYYKLVKKVEADPEFDTKRAKKKLRRYVESHD
                     HAIRLKAEIMVDHFHEQVLALNKIGGQARSMVVTSGIERAIQYYHAFRDYLKERKSPY
                     QAIIAFSGEHEYGDAKVTEASLNGFPSSQIADKIQEDPYRFLICADKFQTGYDEPLLH
                     TMYVDKILSGIKAVQTLSRLNRAHPQKHDVFVLDFMNDSDTIQEAFADYYRTTILAEE
                     TDPNKLHDLKAALDGDQVYAQTQIDQLVGLYLSGADRDKLDPILDASVAVYKNQLDED
                     GQVDFKSKAKAFLRAYGFLSSILPYTNAEWEKLSIFLNFLVSKLPAPKEEDLTKGILE
                     TIDMDSYRVEKKATMKIQLPGENAEIEPLPMTGGG"
BASE COUNT         1474 a         1600 c         1721 g         1355 t
ORIGIN      
        1 cgaaacggga tgagaaagca accgaagtcg ttagcaaaat ccataagccg ggcaaggtca
       61 agcccgatcc gcttcgcggt ctcttcgaga tcaaactcga ttgcaagccc gctgtcgtcg
      121 aatacgagcc tgattccgag cttcgcgata cggaacagat tcctctgctg gaagagggcg
      181 gcatcgaagc cttcatccgt cgagaagtcc tgccgcacgt gctcgacgct tggtatgacc
      241 cagaaagcgt aaagaccggc tacgagatca gcttcacccg ctacttctac aagccacagc
      301 ctcttcggtc actcgaagaa attcgagccg acatcctggc gttggagagg gagactgagg
      361 gactgcttgg ggagatcatc ggggaagtca cacaatgatc gctgatctca aaccctattc
      421 ggcttacaag gactccggcg tgccgtggtt aggtaaagtg ccttcaacgt ggagcctcgc
      481 gcgcaatggt gggttgtttg tacagcgaaa tgagacggga ttcgtccatc ttccgatctt
      541 ggaggtatca ctcaaaaccg gcgtccgagt gcgaaacttt gatgaaagcg ggcgaaagca
      601 ggtgatgtct gatcgagaca agtacaagcg agctcgtaag ggcgatcttg cctacaacat
      661 gatgcgaatg tggcagggtg caataggaat ggcaccgacc gatggccttg ttagtcccgc
      721 atacgtcgtc acccggccgt tacgaggtgt cgaaccgaag ttttatttga atttgttcag
      781 gatcgatgcc tatatgggcg aggtcgacaa gttctctcac gggatcgtca aggacagaaa
      841 ccgcctctat tgggaagatt ttaagcaaat gccttcgccg gttccgccga cggacgaaca
      901 agctgccatt gtccggttcc tcgaccatgc ggaccgacgg atcaagcggt acattcgtgc
      961 caagcagaaa ctgatcaagc tattggagga gcagaagcag gccattattc atcgcgccgt
     1021 cactcgtggc ctcgatccta acgtccgcct caagccctcc ggcgtggagt ggttgggaga
     1081 tgtgccagag cattgggaag tggtccggct taaaacactt tgttcgatga agagcggcga
     1141 cagcattacg gcgatgtcga tcgaaccggt gggcaagtat ccggtatatg gtggtaacgg
     1201 aatccgtggg tacacatcta cctacacgca cgacggcgat tttgcccttg tcggtcgtca
     1261 aggggcattg tgcggtaacg tgcacctagc ctctggccgc ttctgggcgt ctgaacatgc
     1321 ggttgtcacg actctgcgtc ccagcaatga cctcaagtgg ttcgcggcat tgctccgcgt
     1381 catgaatttg agtcagtact cgatagcagc cgcccaaccg ggcctggcag tagagcgtat
     1441 cctgaacctc tctgtacccg caccacccat gtcggagcag agtcgaattg cggtccacat
     1501 ggatatgaca gcagtggggc tggtagcggc catccagagg tcaggccgcg agatcgaact
     1561 cctccgcgag tatcgcactc gattgatcgc cgacgtagtc accggcaagc tggacgtgcg
     1621 agaggcggcg gccaagttgc cggatgagcc ggacgagcct gaatcgtttg atgaggtaga
     1681 tgtgcccgtg gatgatgatg aaaaagcgga gggtactgat ctcgatgccg ttgccgatga
     1741 ggttgaggct tgagtccggt tccggcggag ccgaagattt atcacatcac gcatgtggat
     1801 aatctcgctg ccatcgtaaa tgatggcgtt cttctttcag atgcagctat cgttaggcgg
     1861 agtggcccga gcgtaaccat tggaatgtcc gagatcaaga ggcgccgaat tgaagagatt
     1921 ccggtatcat gccatccagg aacgaggatc ggagactacg ttccattcta tttctgtccc
     1981 cgatctgtga tgttatttct gattcattgt gccaatcacc ctgatctgac atatcgtgga
     2041 ggacagggac cagttgtcca cttggaagct gacatgcatg aagttgtaag ctgggctgag
     2101 cagggagcta gaaagtgggc ctttagcctg tccaatgccg gcgcgtatta cgtccaagtt
     2161 cggggtcgcc ttgacgaatt agaagagatc aattgggcag ccgtgtcagc cacggacttt
     2221 cgctcacctg atgttaagga aggtaagcag gctgaatttc tggttcacgg ctcattccca
     2281 tgggagttag tgaggcgggt cggcgtctgt tcggccagaa ttaaggctca agccgaggcg
     2341 gctctccaaa ttgctgccca taggcctcct gttgaaattc ttcgtgattg gtattattga
     2401 ggaggagctc tccccatgat tgaacctacc actggcaata tcctcgctgc tgacgcggaa
     2461 gcgttggtta atacggtcaa ctgtgttggg atcatggggc ggggtattgc tcttcagttt
     2521 cgcaaggcct tccctgataa cttcaaagcg tatgaagcgg tctgcaagcg tggtgagttg
     2581 cagccagggc atatgtttat tttcgatacc ggcagtctta ctaacccgcg atacatcatc
     2641 aacttcccga cgaagagaca ctggaagggg aaaagccggt tggccgatat tgaattggga
     2701 cttacggccc ttgtgtcaga tattcatcgg cttggcattc gctcaattgc aattccgcct
     2761 ctaggctgtg ggcaaggggg acttaattgg gcagatgtgc gtccaagaat tgaacgagca
     2821 ttggctgctg taccggatgt gggagtgcta ttgttcgagc cgaccggcaa ctctgtgcat
     2881 tcagcatcaa caccgtcccg ctcgatacct aaaatgaccg aagcgcgtgc cgcgttggtg
     2941 ggattaatgc aacgctacct tgctgggctg atggatccct tcattagcct actggaagtg
     3001 cataagctcc tctatttcat gcaggaggcg ggagagagtt ctctgcggct tcggtatgtg
     3061 aaggctccat atggtccata tgcggaaaac ctacgacacc ttctcttgag tgtggaaggc
     3121 tatctcacct ccggttatac cgatggagtg gacgatcccg agaagccttt cgaacttgtg
     3181 cctggagcaa ttgccgatgc cgaacacttt ctggaaggac atccgcatac acaacatcgt
     3241 ttccagcggg ttgctgacct cgtcaaaggg ttcgagactc cattcggtct tgagttgtta
     3301 tccacagttc attgggtttc aacacgggag ggtgctctga ccacggatga ggctctagcg
     3361 gcgacattta gatggaacga gcggaagcga cggttctcac ctcagcagat cgctcttgcc
     3421 tggaatgtac ttcgtgacaa gggctgggta tccgctcaac cagctgcgca tcacgcgtga
     3481 agaccgacac cagtgaaaag ggccttgaaa atctcatcgt cgcggcgatg gctggccaag
     3541 cttctgacga agggccgcca tctggcgagg gtattagtct cgttcgggaa cgatacggcg
     3601 gtaccggatg gattcccggc caagctgagg actatgaccg cgagttctgc atagatcttc
     3661 cacagctccg cgccttcctg cacactaccc agccgaaagt tgccgaagca ttcgacctta
     3721 ataacgatag cccgatccgg cgtaagcttc tcgctcgact ccaaggagag atctcaaagc
     3781 gtggcgtcat cgatgtcctg cgtcatggaa tcaaggacgg tccacaccat gtcgatttat
     3841 tttatggcac accttcgccg ggcaatgaga aggccgctac actgaatgcg tcaaaccggt
     3901 tcacagtcac ccggcagctt cgctatagcc gcgacgaaac acaacaagct cttgacctcg
     3961 ctttgttcat caatggtctt ccgatcgcga cgttcgagct gaaaaatagc ctgaccaagc
     4021 agacggttga ggacgcggtc gagcagtaca agcgtgaccg tgattcacgc gaaaaactct
     4081 tcgagtttgg ccgttgcgtg gtgcacttcg ccctggacga tcatgaagtg cgcttctgca
     4141 ctcatctaaa agggaaaagc tcctggtttt tgcctttcaa tctaggctgg aatgacggag
     4201 cgggcaatcc tcccaacccc tacgggatta agaccgacta tctgtggcac aagattctta
     4261 ctcgcgacgg gatgacagac attcttgaga actatgcgca gatcgtagaa gagaagaatg
     4321 agaagaccgg caagaagaag gcggtgcaga tctggccacg gttccaccag ctcgacgtgg
     4381 tacgcaaact cctttccgac acgcgacaac agggtggggg tctgcggtat ctaattcaac
     4441 attcagccgg cagcggcaaa tccaattcca tcgcctggct cgcacatcaa ctcattggtt
     4501 tacagaaaga tgacgcactg gtcttcgact cgatcattgt cgtaacggac cggcgcattc
     4561 tcgataagca aatccgtgac agcatcaaac agtttgctca ggttggtgcc accgtcgggc
     4621 atgctgagca ctctggcgac ctgcgcaagt tcatcgccga ggggaagaag atcatcatct
     4681 ccacggtgca gaaattccca ttcattctgg atgagatcgg cagcgagcag cgtggtagga
     4741 agtttgcaat tatcatcgac gaagcccatt caagtcaggg cgggcggacc tctgcggcgc
     4801 tcagcatggc gctttcgacc gcaggcgccg aggaggacga cgaaactact gaggacaaga
     4861 tcaaccggat tatggaagcc aagaagctct tgcccaacgc cagctacttc gctttcaccg
     4921 ccactccgaa aaacaagacg ctggaaatct tcggcgatgc acttccgcct gatgccgaag
     4981 ggaaggtccg ccaccaaccg ttccacagct atacgatgaa gcaagcgatc caggaaggct
     5041 tcattctaga cgtactgaag cactacacac aggttgagag ttattacaag cttgttaaaa
     5101 aggtagaagc cgatccggaa ttcgatacca agcgagcaaa gaaaaagctt cgtcggtatg
     5161 tggaaagtca cgatcacgcc attcgcctca aggccgaaat tatggtggat cattttcatg
     5221 agcaggttct ggcgctgaac aagatcggtg gacaggccag gtcgatggtt gtgacgagtg
     5281 ggatcgagag ggccatccag tactaccacg ccttccgcga ctatctgaaa gagcgcaaga
     5341 gtccctatca ggctatcatt gccttttcgg gggagcatga atacggtgac gcgaaggtga
     5401 ccgaggcttc tttgaacggg tttccatcga gccagatcgc ggacaagatt caggaggacc
     5461 cataccgctt tctgatttgc gccgacaagt tccaaacggg gtacgacgaa ccgctgctcc
     5521 acacgatgta cgtggacaag atcctctccg gtatcaaggc ggtgcaaacg ctctctcgcc
     5581 tcaaccgcgc acatccacag aaacatgacg tattcgtgct cgatttcatg aacgacagtg
     5641 acaccatcca agaggccttc gccgactact accgcacgac gatccttgcc gaagagactg
     5701 atcccaataa gctccacgac ttgaaggcgg cgctcgatgg cgatcaagtc tacgctcaaa
     5761 cacaaatcga ccaacttgtg gggttgtacc tgagcggggc cgaccgggat aagctcgatc
     5821 cgatcctgga tgcctctgtc gccgtctata agaatcagct cgacgaagat gggcaggtgg
     5881 atttcaagag caaggccaag gcttttttgc gagcctatgg tttcctctct tcgatactgc
     5941 cctataccaa tgcggagtgg gaaaaactgt cgatcttcct caacttcctt gtatccaagt
     6001 tgccggcccc gaaggaagag gatctcacca aagggatctt ggaaaccatc gatatggaca
     6061 gctatcgcgt tgagaagaag gccacgatga agattcagtt gccgggcgag aatgccgaaa
     6121 tcgagccgtt gccaatgacc ggtggaggaa
//
LOCUS       DATNDD010000298         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3518563, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000298 DATNDD010000000
VERSION     DATNDD010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518563"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VN647_07155"
     CDS             complement(<1..589)
                     /locus_tag="VN647_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWG96846.1"
                     /translation="MLTSSFIFLPGVGPVTERRWWQAGLCNWQSFLDRPSIAGLSEHR
                     KIWYDDELREAQSLTEKGHMQMFGSRFRRREHWRLYKTCRSRTGYLDIETTGAPPESG
                     EVTVVGLHREGNTTTLVRGENLTMERLQAELDHCNLLVTFFGSVFDVPYLCAKFPGLR
                     FPTLHFDLCFAARRLALRGGLKHIEQEMGIDRNTTI"
     gene            685..1068
                     /locus_tag="VN647_07160"
     CDS             685..1068
                     /locus_tag="VN647_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HWG96847.1"
                     /translation="MTAPGKRIRIIVGGVQLEAELRKTKTADEVYAALPVTAAISTWG
                     EEFYFPIPGVRDYRETATTQVKVGDIAFWGVGKVLAIFFGRTPMSLGADPVPADRVNI
                     IGRIVGDATQFRRVMEAPTIHLEAG"
     gene            1074..1355
                     /locus_tag="VN647_07165"
     CDS             1074..1355
                     /locus_tag="VN647_07165"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWG96848.1"
                     /translation="MRLSKERVRHMAESLTGRLQEEGHIELVGERKGFVEALDQAITH
                     ELSAEDRVNAEVRQLLTAYEHQIAQGQVDYQKMFQMVKQKLIRERGIIL"
     gene            1457..1732
                     /locus_tag="VN647_07170"
     CDS             1457..1732
                     /locus_tag="VN647_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWG96849.1"
                     /translation="MLSEDKVSHLSHVIFTAVKQHAGAKVKSDDARLLKEIKRVLAAE
                     LAQEQEIDRKVKAKLASYSRGIIEGSNEWDVLYRKTFEEEMRKQTKV"
     gene            1752..2210
                     /locus_tag="VN647_07175"
     CDS             1752..2210
                     /locus_tag="VN647_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96850.1"
                     /translation="MKKTLHRAVIAGIVLVLVGMTACSQKRTPLVPLVLEGEVKSQAA
                     TLTEQGTQAYQAKQFEEAKRYFEQAVAAEPQSGQAHYNYALALNALGDSEVARQHFIE
                     AADLAPGDKVIWDSPALSPYGNPENKNKAKLGPNMPRRSSFGSGMPAGGY"
     gene            2232..2699
                     /gene="bcp"
                     /locus_tag="VN647_07180"
     CDS             2232..2699
                     /gene="bcp"
                     /locus_tag="VN647_07180"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HWG96851.1"
                     /translation="MSKELAVGDQAPELSIPDQHGKTMTLKSFKGKQIVLYFYPKDDT
                     PGCTKESCDFRDVESQILRAGGAIVGVSLDGKESHQKFIKKFGLPFPLLSDENAAISK
                     AYGVYKEKNMYGKKCWGIERSTFVIDPEGKLKAIFRKVKVDGHADEVLMALKA"
     gene            2711..>3421
                     /locus_tag="VN647_07185"
     CDS             2711..>3421
                     /locus_tag="VN647_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96852.1"
                     /translation="MTVQSTAAFLRIVRFSLISLSAVFIGTSLLHAATEKASATILVK
                     DAFTSPNQAATIEAKLISKGILTSNPLGGEPVELVVNGKVAATAMTGGDGKAFFTYMA
                     KAIGLAPVQVRVGNSPRVAPTEGQANLAVWEKRHPILTIELSSLMEELTPSRVPRIGV
                     AIESERKPMPEAADELVKLTQFYYRVIYVVTSPTGRDGFQATTEAREWLKTNKFPTGY
                     VMALPPDSQALGAKIDEFH"
BASE COUNT          850 a          857 c         1018 g          696 t
ORIGIN      
        1 tgatggtcgt gttgcggtcg atccccattt cttgctcgat gtgcttcagc cctccgcgta
       61 acgcaagtcg acgggcggca aaacagaggt cgaagtgcaa tgttggaaac cggaggccgg
      121 gaaacttggc acagaggtag gggacatcaa agaccgatcc gaaaaacgtc acgagtagat
      181 tacagtgatc gagttccgcc tgcaaccgct ccatcgtcag gttttcacca cgaaccaggg
      241 tcgtcgtgtt cccctcgcga tgaagtccca cgacagtcac ctctcccgat tctggcggcg
      301 caccggtcgt ttcgatgtcg agataacctg tccgagatcg gcaggttttg tacagtcgcc
      361 aatgttctcg gcgtcgaaag cgagagccaa acatctgcat gtgtcccttc tccgtcagcg
      421 attgcgcctc tcgaagctca tcatcgtacc agatcttgcg gtgctcggag aggccggcga
      481 tggacggtcg gtccaggaaa ctttgccaat tgcacaagcc cgcttgccac cagcggcgtt
      541 ccgtgaccgg tccaacgccc ggtagaaaga tgaagctgga ggtcagcatt tcagggattg
      601 tagccttgat ttcgcatagg agacaagcta gagttcgcag catcgcgaag aatgcagtga
      661 agaaacgagt aggccatcga tcgcatgacc gcaccgggga agcgaattcg tattattgtg
      721 ggtggagttc agctggaagc cgaactcaga aagacgaaga cggctgacga agtctacgcg
      781 gcacttccgg tcacggccgc gatcagcacg tggggagagg agttctactt cccgattccc
      841 ggagtccgcg actatcggga aaccgcgacg actcaggtga aggtcggaga cattgccttc
      901 tggggggtag ggaaggttct ggcgatcttt tttggaagaa cccccatgag tttgggggcc
      961 gatccggtcc cggcagatcg ggtgaacatc atcgggagga tcgttggaga tgcgacccaa
     1021 tttcgacggg tgatggaagc cccgacgatt catctcgaag cggggtgagt gcgatgcgac
     1081 tgtcgaaaga gcgtgtgcgg catatggccg agagcctcac cggtcgtctc caagaggaag
     1141 gacatatcga actagtcgga gagcgaaagg gattcgtcga ggcattggat caggccatca
     1201 cgcatgagtt atccgctgag gatcgtgtga atgcggaagt gcggcaactg ctgacggcct
     1261 atgagcatca gattgcacaa ggccaagttg actatcagaa gatgttccag atggtgaaac
     1321 aaaagttgat tcgtgaacgg ggtattattc tgtgaaagtc aatggtcatg gggtcatgag
     1381 gcaagaaacg aatcggttat cgtaaagttt cagctgccgt tggccggtga cgagtgacca
     1441 ttgacttgga ctcaagatgt tgagcgagga caaagtcagt catctgtccc atgtgatctt
     1501 cacggccgtc aagcaacatg ccggagcgaa ggtcaagagc gatgatgcaa ggttgttgaa
     1561 agagatcaag cgcgtgttgg ccgccgagtt ggcccaggaa caggagattg atcgaaaagt
     1621 aaaagccaaa ctcgcatcct attcccgggg cattattgaa gggagtaatg agtgggatgt
     1681 gttgtatcga aagacgttcg aggaagaaat gcgcaaacag acgaaggtct gaaggctgag
     1741 gtcgcgttgc catgaaaaag accctacacc gagcggtaat cgcaggtata gtgctggtgc
     1801 ttgtcggcat gacggcatgt tcgcagaagc gtacgccgtt ggtgccgctt gtgcttgagg
     1861 gcgaggtgaa atctcaagcg gccacgctga cggaacaggg cacacaagcc taccaggcca
     1921 aacagtttga agaggccaaa cgatactttg aacaagcggt cgccgcagaa cctcaatccg
     1981 gccaggccca ctataactat gccttggcgt tgaatgcgct tggagattct gaagtcgcgc
     2041 ggcagcactt tatagaggct gccgacttgg caccaggaga caaagtcatt tgggactcac
     2101 cggcgctttc gccatacggg aatcctgaaa ataaaaacaa agccaaactg ggtcccaata
     2161 tgccgcgccg gtcgtcgttc ggcagcggca tgcccgccgg cggttattaa cttccggata
     2221 aggatggatc tatgagcaaa gagcttgcgg taggagatca ggcaccggaa ctctcaatcc
     2281 cggatcagca tggaaagacg atgaccctga agagcttcaa gggcaaacaa atcgtgttgt
     2341 acttctatcc gaaagacgat acgccgggat gtacgaaaga gtcctgtgac tttcgcgacg
     2401 tcgagtcaca gatcctccgg gccggaggcg ccatcgtcgg cgtgagcttg gatgggaagg
     2461 agtcccatca gaaattcatc aagaaattcg gactgccctt tccgctcctc agtgatgaaa
     2521 atgcggcgat ctccaaggcc tacggtgtgt acaaagagaa gaacatgtac ggcaagaagt
     2581 gttggggtat tgaacggagc acgttcgtta tcgatcccga aggcaagctg aaggcgatct
     2641 ttcggaaggt gaaagtcgac ggccacgcgg acgaagtgct tatggcactg aaggcctaac
     2701 cgaagggccg atgactgtgc agtcaacggc cgcttttcta cgcatcgtca ggttttccct
     2761 catttctctc tccgccgttt tcatcggcac gtctcttctc catgctgcta cggagaaagc
     2821 atccgcaacg attcttgtga aggatgcgtt cacgtccccc aatcaagcgg cgaccattga
     2881 agccaaactg atctcgaaag gtattttgac gagcaaccca ctgggaggag aaccggttga
     2941 gctggtggtg aatggaaagg ttgccgccac ggccatgacc ggtggggacg gcaaggcgtt
     3001 cttcacctat atggcaaagg ccataggtct agcgcccgtc caggttcgcg tcggcaacag
     3061 cccccgagtg gctccaacag aagggcaggc caaccttgcc gtatgggaaa aacggcatcc
     3121 catcctcacg attgaactat cttcgctgat ggaagaactg acacccagtc gagttcctcg
     3181 catcggagtt gcgattgaat cggagcgaaa gccgatgccc gaggcagccg atgaactcgt
     3241 gaagctcacc cagttttatt accgagtcat ctatgttgtg acatcgccaa caggcaggga
     3301 tgggtttcag gccaccacag aggccagaga atggttgaag acgaacaagt tcccgaccgg
     3361 ctatgtgatg gcgctcccgc cggattccca agccttaggg gcaaagatcg acgaattcca
     3421 t
//
LOCUS       DATNDD010000299         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2132045, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000299 DATNDD010000000
VERSION     DATNDD010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132045"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1380)
                     /locus_tag="VN647_07190"
     CDS             complement(235..1380)
                     /locus_tag="VN647_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HWG96853.1"
                     /translation="MKKTLLWMVGLTLILSCFVVAPRAHAQAKEIDVTGNWQGTLQAG
                     QGLRIVMKITKADGKLKGISYSIDQGGQPIPLNSITVHGTAFNFAISMLDVTYAGTLS
                     ADGKTITGNQTQGGHTAVMNFEHVTPEATWAIPEPPKAMAADAVLKFEVITVKPSDPN
                     RPGKLFTIRGRHLMTINTTMNDMVTFAYGLHVKQIIGAPEWFATDKFDLDGVPEAEGR
                     PNTKQMKELIQSALTDRFKLTFHHDQKELSVYALTVAKGGPKMTETIHQPNDPKNFIF
                     RGKLGDLMVTNSTMKDFCDGMQSAVMDRPVVDHTGLTARYDFNLNWTPDDSQFAMFGP
                     RPTPKDDPNAPPSLYTALQEQLGLKLEATKANADVFVIDHVEKPAAN"
     gene            complement(1560..1745)
                     /locus_tag="VN647_07195"
     CDS             complement(1560..1745)
                     /locus_tag="VN647_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96854.1"
                     /translation="MVDIDLIVDTLRAHGHKVEDVHHVPHDAGEYEMIIDGDVVDLEG
                     ARHVLELDGPRRRQARA"
     gene            complement(1794..>2174)
                     /locus_tag="VN647_07200"
     CDS             complement(1794..>2174)
                     /locus_tag="VN647_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96855.1"
                     /translation="GGSPLMLQDIDSACQAAGVWPHAVFSGHAHNYQRFTRTVNNLQI
                     PFLVAGCGGHSPLSPMRGTYRTPYKIDDTLTLESYNTTDYGYLRVVVNAQTMRIEFHP
                     ESDGGTVKTPNDAVTVALATYQVN"
BASE COUNT          434 a          553 c          685 g          502 t
ORIGIN      
        1 tgcagatgaa atccctgtat gggacttggc ctgaagtgat cctccagccc acatcgatga
       61 gcgggctaag atcatatccg ccaaaacgat agatgcctat tcttagattg gaacagcatg
      121 cgaacactgt agcgaaaata atcccaataa atccaagcca ataaagtcgc ttaaacatat
      181 aagtgaaatc ataagtcaca gggcagattc aaagccggga gaaccatcga ggtatcagtt
      241 ggcggcaggc ttctcgacgt ggtcgatgac gaagacgtcg gcgttggcct tggtcgcctc
      301 aagttttagc ccaagctgct cctgtagtgc ggtatagagg ctcggcggcg cattggggtc
      361 gtctttcggt gtgggtctgg ggccaaacat ggcaaactgc gaatcgtcgg gtgtccagtt
      421 gaggttgaag tcatacctcg ccgtaagccc ggtgtgatcg acgaccggcc tgtccatcac
      481 cgcggactgc atgccatcgc agaagtcctt catggtcgag ttcgtcacca tcaagtcgcc
      541 gagcttccct ctgaagataa agttcttcgg atcgttgggc tggtgaatgg tctcggtcat
      601 cttcgggccg ccctttgcga cggtgagcgc gtagacggag agctccttct gatcgtggtg
      661 gaaggtgagc ttgaagcgat ccgtgagagc ggactggatc agctccttca tctgcttggt
      721 gtttgggcgc ccttcggctt cgggcacgcc gtcgagatcg aatttatcgg tggcgaacca
      781 ctccggcgca ccgatgatct gcttgacgtg cagcccatag gcgaaggtca ccatgtcatt
      841 catggtggta ttgatcgtca tcagatggcg gccacgaatg gtgaagagtt tgccggggcg
      901 gttgggatcg ctgggcttta ctgtgatcac ttcaaacttg agcacggcat cggcggccat
      961 cgccttagga ggctcgggaa tcgcccaggt agcctcgggc gtgacgtgct caaagttcat
     1021 gacagccgtg tggccgccct gagtctgatt accggtgatg gttttgccgt cggcgctgag
     1081 ggttccggcg taggtgacgt ccagcatcga aatcgcaaag ttgaacgccg ttccgtgtac
     1141 ggtgattgaa ttgagaggaa tcggctggcc gccctggtcg atgctgtaag agatgccctt
     1201 cagcttgcca tcggccttgg tgatcttcat gacgatgcgc aagccctgac cggcctgcag
     1261 ggttccctgc cagttgccgg tgacgtcgat ctctttggcc tgggcgtggg cgcgcggcgc
     1321 gacaacgaag caggacagga ttaacgtgag cccaaccatc cacaacagcg tcttcttcat
     1381 atgcagttcc cccgattggg ctaacggacg atgcatcgca gagaccgcag tgacggcctg
     1441 aagcgaagtc gggcaattct atcaatagat agtgggagtt atggatgaat tttggttgga
     1501 ttcgcaaagc gacagagtca tcgcatgggt tgtgcgccgg agggtgaagg cgccgcgagc
     1561 tatgcccggg cttgccttct ccttgggcca tcgagctcga ggacatgccg tgcaccttcg
     1621 agatcgacga cgtctccgtc gatgatcatc tcgtattctc cggcgtcgtg cgggacatgg
     1681 tgcacatctt caactttatg cccgtgggct cgaagcgtat cgacgataag gtcgatgtct
     1741 accatatgtc ctttagagga gggcggtcgg gcgaaggttg tttccgtgcg acatcagttg
     1801 acctgatacg ttgccagtgc gaccgttacg gcatcattcg gggttttgac ggtgccgccg
     1861 tcgctctcgg ggtgaaactc gatgcgcatg gtctgcgcat ttacgacgac gcggagatat
     1921 ccgtaatctg tcgtgttgta gctttcgagt gtcagggtgt cgtcgatctt gtaaggcgtt
     1981 cggtaggttc cacgcatggg cgagagtgga gagtggcctc cgcatccggc gacgaggaag
     2041 gggatttgaa gattgtttac cgtgcgcgtg aaccgttgat agttgtgagc atggccggag
     2101 aagacggcgt gcggccagac gcccgcggcc tggcaggcgg agtcgatgtc ctgcaacatt
     2161 aaggggctgc cgcc
//
LOCUS       DATNDD010000300         3956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1225892, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000300 DATNDD010000000
VERSION     DATNDD010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225892"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..774
                     /locus_tag="VN647_07205"
     CDS             136..774
                     /locus_tag="VN647_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96856.1"
                     /translation="MNFCAILLLLAFTTPSWGKDIEAFPDLNLNNPNELKEATRVLEE
                     ELKLAARPQTYLLIDLGGSTIHIKSRGIGLHQIPIIAWSAGSREDLKGIHRLVARPSV
                     VRRKIEPGAGAEQEPISLADMPADYVLSFRPSLSVTVVPSVAGDNLLQSAVLLGKVGW
                     RNVKDWTSRLLTDHSSDPTSHLRLTLSVDHAQSLAWSLVDGMALVIRRPNDK"
     gene            888..1151
                     /locus_tag="VN647_07210"
     CDS             888..1151
                     /locus_tag="VN647_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96857.1"
                     /translation="MWLNDKLPPSFQSIVKPAFEALSAQVIDRLWAKDHRLWKPDPKE
                     IADRLGWLTVPDQMRQQLGKLQCCAIWVRQVTKAISPRGHSFS"
     gene            1201..1476
                     /locus_tag="VN647_07215"
     CDS             1201..1476
                     /locus_tag="VN647_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96858.1"
                     /translation="MEPSVEGEGEPWRKAVCRNDRYGYRLRKDGPDYGFGEIFTNPPD
                     IGGRYSVLSLFGLVPAALLGLDIARLLERATGMAGLCRQQKDIEGAS"
     gene            1494..2270
                     /gene="pgl"
                     /locus_tag="VN647_07220"
     CDS             1494..2270
                     /gene="pgl"
                     /locus_tag="VN647_07220"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWG96859.1"
                     /translation="MPVTPKIYVSRNVQEWATDATAFLLSISEHAIQSTGRCLIALSG
                     GTTPKTLFRTLREPEWSSRFNWSKIFFLFGDERCAPPECPESNFGMAQTELFQPLNIR
                     PEHIYRMKGEYEDPTAAAHEYEETIRRVTRCPAPDPPCLDLILLGLGEDGHTASLFPG
                     TSALQEQSKIVTVGHAPTGIRSRLTLTLGVLNHAAVVLFLVTGSGKAEMVRRVIESES
                     EADHSLPAARISPEPGRLVWMLDQAAAQQLTKKRSHREGP"
     gene            2267..3337
                     /gene="glk"
                     /locus_tag="VN647_07225"
     CDS             2267..3337
                     /gene="glk"
                     /locus_tag="VN647_07225"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HWG96860.1"
                     /translation="MILAGDIGGTKTNLALYEWTTERVEPLRLESFPSGDYKTLEDIL
                     IEFLTLPKEPSSVASPETQGETQSEKLVTPPAEPVKLTASCLGIAGPVIDNRCQTTNL
                     PWVIDGRTIAKQFNIPRVQLLNDLEATAYGLLWLRPDELEILNAGNPPNKRQALALIA
                     AGTGLGEGILFWDGKTYRPMPSEGGHTDFAPNNDQEIELLRYLRTQYLHVSYERILSG
                     PGLHAIYEFLRDTKKNEPTWLAEKIRAGNPAAEISQAGLQGQAEIAKQALDLFTSIYG
                     AEAGNLALKALSLDGVYVGGGIAPKLITKLQDGTFMKAFTNKGRYKRLMSQMPVKVII
                     NQQTALIGAASVAAALSLASPS"
     gene            3406..>3956
                     /gene="rpiA"
                     /locus_tag="VN647_07230"
     CDS             3406..>3956
                     /gene="rpiA"
                     /locus_tag="VN647_07230"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWG96861.1"
                     /translation="MIVGLGTGSTAKHLLIALGERVRAGMKLRGVPTSQETAMLARES
                     GIPLIDTENRWEIDVAIDGADQVDPEFNLIKGGGGALLKEKIVAARAKQFIVMVDYTK
                     QVPVLGGSFPLPIEIIPFGWGSTAREVEVLTKSRVVLRECDGAPFKTEAGHLIVDVHI
                     DRIDRPGELETALNLIPGVVETGL"
BASE COUNT          952 a         1071 c         1060 g          873 t
ORIGIN      
        1 cgtttgttgg gaaaaaacgt gacgcatgga tgcatccggc tcaatgacag cgatcttaaa
       61 ggtgtctaca agctcgctcg agtagggaca ccgatcatga tcttttgatc cctcaagagc
      121 gcgcagatat tacccatgaa cttctgcgcc attcttctcc tgcttgcctt caccacacca
      181 agttggggaa aggatatcga agcctttccc gacctgaatc tcaacaaccc caatgaactc
      241 aaagaggcaa ctcgcgtcct tgaggaagaa ctcaagctgg ctgctcgtcc ccaaacctat
      301 ctcctgattg acttgggcgg gagtacgatt cacattaaaa gtcgtggaat cggcctccac
      361 cagattccca tcattgcctg gtccgccgga tcacgggagg atctgaaagg gatccaccga
      421 ttggttgcac gaccttcggt cgtcagacga aagattgaac ctggtgcagg agcagagcag
      481 gagccgattt cactcgctga tatgccggcc gactacgtgt tgtctttcag accctccctg
      541 agcgtcactg tagttccatc ggttgccggt gacaatctcc ttcaaagcgc cgttctactg
      601 gggaaagtgg ggtggcgaaa tgtgaaagac tggacaagca ggctcttaac ggatcactca
      661 tctgatccta cgtctcatct tcggctcacc ctctcggtcg accatgcgca atccctggcc
      721 tggtctctgg tcgacggcat ggcgttggtc attcgtcgcc caaacgataa ataggaaagc
      781 agagcataga gtccctgttg ccttttctaa accggctcaa taagatatga acgagttacc
      841 caccaactct accactgaga ttccgttgaa gaaggaggcc cctgtctatg tggttgaatg
      901 acaagcttcc accgtcgttt caatccatag tgaaaccggc cttcgaggcg ctcagcgcgc
      961 aggtcattga ccggctgtgg gcaaaggacc atcgactctg gaagccggat ccaaaggaaa
     1021 tcgccgaccg tctaggctgg ttgacggtac cggatcagat gcgacaacag ctgggaaaac
     1081 ttcaatgctg tgctatttgg gttcggcagg tcacgaaggc tatttcccct cgcggacatt
     1141 ctttctcgta gccagcaagt ccgggggcac gatcgaagtc atatcgctct tcgctcactt
     1201 ttggaaccta gtgtcgaggg cgaaggggaa ccatggagga aggcagtttg tcgcaatgac
     1261 cgatacgggt accggcttag aaaagatggc ccggactatg gatttggaga aatcttcacg
     1321 aacccacctg atatcggcgg acgatattcc gtgctttctc tgttcggtct tgttccagca
     1381 gcactccttg gcctcgatat cgccagactc ttggaacggg caaccggtat ggcgggatta
     1441 tgccgtcagc agaaagacat tgaaggagcg tcctaagcgc tctcaacgtt gatatgcccg
     1501 tcaccccgaa aatctacgtc tcgcgcaacg tgcaggaatg ggcgacggat gcgacggctt
     1561 tcctcctgtc cattagcgag catgcgattc aatccaccgg ccgctgtcta atcgcactct
     1621 ccggaggaac tactccgaag actctcttcc gaaccttacg cgaaccggag tggagctcgc
     1681 gtttcaattg gtcgaaaatc tttttcctat ttggagacga acgatgcgcg ccacctgaat
     1741 gtcccgagag caactttgga atggctcaaa cggagctgtt tcagccattg aacattcggc
     1801 cagaacatat ttatcgaatg aagggtgagt acgaagaccc gacagctgcg gctcatgaat
     1861 acgaggaaac gattcgaagg gttacccgat gtcctgctcc agatcctcct tgcctagact
     1921 tgatccttct tggcctcggc gaggacggcc ataccgcatc gctttttcct ggaacctctg
     1981 ctctacagga acaatccaag attgtcacgg ttggtcacgc tcccacaggc atcagatctc
     2041 gtcttacctt gaccctaggt gtgctgaatc atgcagctgt ggtactgttt cttgtgaccg
     2101 gctcaggcaa agcagagatg gtccgccggg tcatcgagtc tgaatccgaa gcagatcatt
     2161 cattaccggc agcgagaata tcaccggaac cgggtcgact cgtgtggatg cttgatcagg
     2221 ccgctgcgca acagcttaca aagaagcgat cacacagaga gggaccatga ttcttgcggg
     2281 agatatcggt gggacaaaaa cgaatctggc gctctatgag tggaccaccg aacgagtgga
     2341 accattgcgt ctggagagtt ttccgagcgg tgattacaaa acgctcgaag acattctcat
     2401 cgagttcctt acactgccga aagagccatc ctccgtagct tcaccggaaa ctcaaggaga
     2461 gacccaaagc gaaaaacttg tcaccccacc tgctgaaccg gtgaagctga cggcgtcctg
     2521 cttgggaatc gcaggaccgg ttatcgacaa tcgttgtcaa acgacgaatc tcccctgggt
     2581 gatcgatggc cggaccatcg ccaaacagtt caatattccg cgcgtacagt tgctcaacga
     2641 tctggaagca accgcctatg gccttctgtg gctgaggcca gacgagcttg agatactaaa
     2701 tgcgggcaac ccgccgaaca aacggcaagc cttggcgctc attgccgccg gcacaggatt
     2761 gggtgaaggt attttattct gggacgggaa gacataccgt cccatgccgt cggagggagg
     2821 ccatacggat tttgcgccca acaatgatca agaaatcgag ctccttcgct atctccggac
     2881 tcaatatctt cacgtgagct atgagcggat tctctccggt cccggcctac atgcgattta
     2941 tgagttctta cgcgacacca agaaaaacga gccgacgtgg cttgcggaaa agattcgggc
     3001 tggtaatccg gccgctgaaa tttctcaagc cggattgcaa ggacaagccg agattgctaa
     3061 gcaagcactg gatctcttca cgtcgattta tggagcagaa gccggcaacc tcgccctgaa
     3121 ggcgctctcc ctcgatgggg tgtatgtggg cggcggtatc gcgcccaaac tcatcacaaa
     3181 gcttcaagac ggcacgttca tgaaagcctt taccaataaa ggtcgatata aacggctgat
     3241 gagccagatg ccggtgaaag tcatcataaa ccagcaaacg gccttgatcg gtgcagcgtc
     3301 tgtcgcagca gccctttctc ttgcttcccc gtcatgacgt cagggaatct cgacagcttc
     3361 aaaagatccg ctgctctaaa ggccgttgaa tttgttcgcg acgggatgat tgtcggacta
     3421 gggacaggat cgacggcaaa gcatctgctc atcgcactcg gcgaacgtgt ccgtgccggc
     3481 atgaaactgc gaggcgtgcc aacgtcgcaa gaaaccgcca tgctcgccag ggagtccggt
     3541 attcctctca tcgataccga gaatcggtgg gagatcgatg tcgccatcga cggagccgat
     3601 caggtcgatc ccgaatttaa tctgatcaaa ggtgggggcg gagcgctcct aaaagaaaag
     3661 attgtggcgg cgcgggcgaa acaattcatc gtgatggtgg actacaccaa acaagtaccg
     3721 gtgcttggag gatccttccc gctgcctatc gagatcatcc cctttgggtg gggaagtact
     3781 gcgcgcgaag ttgaagtgct caccaaaagc cgcgtggtac tcagagagtg cgatggggcg
     3841 ccgtttaaga ctgaggctgg tcatcttatt gtcgacgtcc atatcgaccg catcgatcgg
     3901 ccgggtgaat tggaaacggc actgaacctc atccccggtg tagttgagac cggtct
//
LOCUS       DATNDD010000301         4498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2355927, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000301 DATNDD010000000
VERSION     DATNDD010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2355927"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2123
                     /locus_tag="VN647_07235"
     CDS             <1..2123
                     /locus_tag="VN647_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96862.1"
                     /translation="VITRDGSGDVGQYTSIAIGADGLGLISYYDATNRDLKVAHCSNQ
                     MCTSATISSLDRTGEVGRYTSIALGSDDLGLMSYYDATNGDLKVAHCVDIACTSATLK
                     TLDHAGDVGQYGSLTIGIDKLPLITYYDGTNGDLKIAHCTDVACEAATITVLTFLQAE
                     ENVGQYTSVTIAGGQGLISYYDATDRYLKSALCPDLSCTEPTLTTTPDKTGDVGRFSS
                     VAIGTDSRPLISYYDATNGDLKVIHCEEVVCVSPTRTVLESEGNVGQYTSIVIGADGL
                     ALISYYDVTNGDLKVAHCGDVACTSATLATPDRTGNVGSSTSVAIGTDGLPLISYYDV
                     TNGDLKVAHCGDVGCTDATVTPLDGAAINVGASSAMVLGADGKAVISYYDATNGDLRV
                     VHCADLACASATLTTVQNQDNVGQDSSVAIGADELPLISYYDATNGDLRVAHCTDVAC
                     TMATTFTLDQAGQVGQFTSMTIGMDGRGLISYYDATNGDLKVAHCDDVACRSAKITIL
                     VSKGDVGQFTSTTVGADGLALISYYDITNEDLKIAHCDDVVCGSATESTLDSRGNVGR
                     YSSVTIGSDGLALISYYDGTNDDLKVAHCIDLTCSSAKVHTLHSKGSIGQFTSVTIGQ
                     DGRGLISYYDAMNGDLRVAHCADVDCETATHNAVQSLDDVGRFTTIITGKDGRALISY
                     YDVTNGDLKVAHCSNSFCVPYTRH"
     gene            complement(2171..3490)
                     /locus_tag="VN647_07240"
     CDS             complement(2171..3490)
                     /locus_tag="VN647_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWG96863.1"
                     /translation="MNDMGRRGFLVRTGLALSAAVLASAYDRASADQQPPQSGFKSWD
                     DLRAQFPLSPQLIHLAAFFLASHPTPVRQAIERHRTGLDSDPIGYWYEHEEKEEAKVL
                     QAAADYLGAQPTDIALTDSTTMGLGLLYGGLRLQAGQEILTTIHDHYSTETSLRLRAE
                     RTGAKVRQISLYRALKTVSRDEIIDSLRKGITSATRIVAVTWVHSNTGLKLPIHEMAQ
                     AIQAINRSRAEQDRVIFCVDGVHALGVEDFRLSELGCDFLIAGTHKWMFGPRGTGLVW
                     GHPHAWPMAQAIIPTFNSQAFEIWLKNGSPHGLPQSIYMTPGGFHSFEHRWALDEAFT
                     LHQTVGRSKVTQRIYELNQQLKQGLAAMPHVTLHTPISQDLSAGIVCFEVAGMAPRAV
                     VEKLRQRGIVGSVTPYATQYARLAPSLLNSPQEIEKTLIEIRNLYSS"
     gene            complement(3608..>4498)
                     /locus_tag="VN647_07245"
     CDS             complement(3608..>4498)
                     /locus_tag="VN647_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HWG96864.1"
                     /translation="RRICQIVHTHGGQVYIDGANMNAMVGLCRLGDIGADVCHLNLHK
                     TFCIPHGGGGPGVGPIGVARHLVPFLPGHPVVKLGAPQAIGPVSAAPFGSPSILPISW
                     MYIALMGRDGLTKATQVAILNANYMVKRLEKHYSILYKGDSGLVAHEFILDLREFKDS
                     AGIEAMDVAKRLMDYGFHAPTVSFPVAGTLMIEPTESEAKSELDRFCEALILIRAEIQ
                     EIIDGRQPRTNNVLKNAPHTAAMVTATEWNRPYSRELAAFPAPWLRSNKFWPSVSRID
                     EAYGDRHLVCSCPPMESYHS"
BASE COUNT          976 a         1279 c         1291 g          952 t
ORIGIN      
        1 cggttatcac ccgggatggc tcaggcgatg tcggtcaata cacttccatc gccatcggag
       61 ctgacggcct cggacttatc agctactatg atgcgacaaa ccgggatctg aaggtggcgc
      121 actgctcgaa ccagatgtgc acctccgcga cgatcagctc cctggaccga accggggagg
      181 tcggccggta cacctctatc gcgcttggga gcgacgacct cggcctcatg agttactacg
      241 acgcgacgaa tggagatctg aaagtcgccc actgtgttga tatcgcctgt acgtcggcca
      301 cgcttaagac gctcgaccat gctggggacg tcggccagta cggttctttg accatcggca
      361 tcgacaagct tccgctcatc acctactacg atggcacgaa cggcgacctg aagattgcgc
      421 actgcaccga tgtggcctgt gaggccgcga cgattaccgt tctcacgttt ctgcaggcag
      481 aggagaacgt cggccaatat acctcggtca ccatcgccgg agggcagggt ctcatcagct
      541 attacgacgc gaccgatcgc tacctgaaat cggcgctctg tcctgatttg tcctgtacgg
      601 aaccgactct cacaacgact cctgataaaa ccggcgatgt gggccgattc agctcggttg
      661 cgatcgggac ggacagtcgc cccctcatca gctattacga tgcgacgaac ggcgatctca
      721 aagtcatcca ttgcgaggag gtcgtgtgcg tttctccgac gcgcaccgtt cttgagagcg
      781 aaggcaatgt cggccagtac acgtccatcg tgatcggagc cgatggcctg gccctcatca
      841 gctactacga cgtgacgaac ggggatttga aggtggccca ctgtggcgat gtcgcctgta
      901 cctccgccac ccttgccacg ccggacagga ccggcaacgt cggctcatcc acgtccgttg
      961 ccatcgggac agacggtctg cccctcatca gttactacga cgtcaccaac ggcgatctga
     1021 aggttgctca ttgtggcgac gtgggttgca ccgacgcgac tgttacccca ttggacggag
     1081 cagctattaa cgtcggcgcg tcctctgcga tggtgctggg ggccgacggc aaggcagtga
     1141 tcagctacta cgatgcgacg aacggcgacc tcagggtggt tcactgcgcg gacctcgcct
     1201 gcgcgtctgc cacgctgacg accgttcaga accaggacaa cgtcggtcag gacagttctg
     1261 tggctatcgg cgcagatgag cttcccctca tcagttacta tgacgcaacg aatggggatc
     1321 tgcgagtcgc gcactgtacc gatgtcgcgt gcaccatggc gaccacattt acgttagatc
     1381 aggcgggtca ggtcggtcag tttacgtcga tgacgatcgg gatggacgga cgaggtctca
     1441 tcagctatta cgatgccacg aacggcgatc taaaagtggc ccattgcgat gatgtcgctt
     1501 gtcgctcggc caagattacc atcttggtca gcaagggcga tgtcggtcag ttcacctcga
     1561 ccaccgtagg tgccgatgga ctggcgctca tcagctacta tgacatcacg aacgaagacc
     1621 tgaaaattgc gcattgcgac gatgttgttt gtggttctgc gacagaaagc acgttggaca
     1681 gtaggggtaa tgtcggccgg tatagttctg tgacgatcgg gtccgatgga ctcgcgttga
     1741 tcagttacta cgatggcaca aacgacgatc tgaaagtagc tcactgcatc gacctaacct
     1801 gcagctctgc caaagtgcat actcttcata gcaagggcag catcggccaa ttcacgtccg
     1861 tcaccattgg tcaagatggc cgtggcctga tcagctatta cgatgccatg aacggggatt
     1921 tacgagtcgc gcattgcgcc gatgttgact gcgaaacggc gacccacaat gccgtccaga
     1981 gcttggacga tgtcggtcga tttacgacga tcatcaccgg gaaggacggc cgggctctga
     2041 tcagctacta cgatgtaacg aacggggatt tgaaagtggc gcattgctcc aacagcttct
     2101 gcgttcccta tacccgtcat tgaaacgaat ctcttccaat accactcggc ttgtgcgtga
     2161 cggctcctat ttacgacgag taaagatttc tgatctctat caaagtcttt tcgatttcct
     2221 gcggtgagtt caggaggctc ggcgcgagcc tggcatactg cgttgcgtat ggtgtcacgc
     2281 tgccgacgat gccgcgctga cgaagttttt ccacgacggc gcgaggcgcc ataccggcca
     2341 cctcgaaaca gacgatcccc gccgaaagat cctgtgatat cggtgtgtgc aaggtcacgt
     2401 gtggcatggc cgccaagcct tgcttcaatt gttgattgag ttcgtagatg cgctgggtca
     2461 ccttggacct cccgacggtt tgatgaagcg tgaaggcttc atccagcgcc caacgatgtt
     2521 cgaacgagtg gaacccgccg ggtgtcatat agatggattg cggaagaccg tgcggtgatc
     2581 catttttcag ccaaatctca aaagcttgcg aattgaaggt cgggataatt gcctgggcca
     2641 tcggccaggc atgcgggtgg ccccaaacga gaccggttcc acgagggccg aacatccatt
     2701 tgtgagtccc agcgatcaaa aaatcacagc cgagttcgct gaggcgaaag tcttcgacac
     2761 ccaacgcgtg aactccatcc acacagaaga tcacccggtc ctgctcggct ctcgagcgat
     2821 tgatggcctg gatcgcttga gccatttcat ggatcggtag tttgagcccg gtgttggaat
     2881 ggacccaggt aacggccacg atgcgtgtgg cggaggtgat gcctttgcgc agggaatcga
     2941 tgatctcgtc tcgggagacg gtcttgagtg cacgatacag ggagatctgc cgaaccttgg
     3001 cgcctgtacg ttcggcgcga aggcggagag aagtttccgt cgaataatgg tcgtggatgg
     3061 tcgtcaagat ttcctgtccg gcttgaagcc gtaagccgcc gtagaggaga cccagcccca
     3121 tcgtcgtact gtcggtcagg gcaatgtctg tgggctgagc acccagataa tcggccgccg
     3181 cctgaagaac cttcgcttcc tctttttctt cgtgctcata ccaatagcct ataggatccg
     3241 agtccaggcc ggtgcgatgt cgttcgatcg cttggcgtac cggcgtggga tgggacgcaa
     3301 ggaagaacgc cgcgagatgg atgagttgcg gagacaatgg aaactgtgcc cgcaggtcat
     3361 cccagctttt gaacccgctc tgcggaggtt gttgatcggc agaagctcga tcatatgcac
     3421 ttgccagaac cgctgcgctt aacgccaagc cggtacgcac aaggaaacct cgacgtccca
     3481 tgtcgttcat acgaacctcc aggtcaaaac tgacggctta ctctcctgct tgtccagaaa
     3541 ggtagacaag aaacgaccgt gactattcca gcgggctaga agacagcagc aataaaggac
     3601 gattctgcta ggagtgatag ctctccatcg gcggacaact acaaacgaga tggcggtccc
     3661 cgtacgcttc gtcgatacgg ctcacgctcg gccagaactt gttgctcctg agccaaggag
     3721 caggaaacgc cgccagttcc cggctatagg ggcgattcca ctccgtcgcc gtcaccatcg
     3781 cggcggtatg aggggcattc ttcagcacat tgttcgttcg cggttggcgc ccatcgatga
     3841 tctcctgaat ctcggcacgg atcaggataa gtgcttcgca gaaccgatcg agctcgctct
     3901 tggcttcact ttccgtcggt tcgatcatga gcgtgcctgc taccgggaac gaaacggtcg
     3961 gcgcatggaa gccatagtcc atcaaccgct tggcgacatc catcgcctcg ataccggcgc
     4021 tgtccttgaa ttcacgcaga tccaggatga actcatgagc caccagtcct gagtccccct
     4081 tgtacaaaat ggagtaatgc ttttccaacc gcttgaccat gtagttggca ttgaggatgg
     4141 ccacctgtgt ggctttggtc aacccgtcac gacccattaa ggcgatgtac atccatgaaa
     4201 tcggcaggat gctcggactg ccgaacggag ctgccgagac aggaccgatg gcttgaggcg
     4261 caccaagctt gaccacggga tgccccggaa gaaacggcac gagatgccgc gccactccga
     4321 tcggtcccac gccaggccct ccgcctccat ggggaataca aaacgtctta tggaggttga
     4381 gatggcagac atcggcgccg atgtctccca gacggcagag gcccaccatg gcattcatat
     4441 tggccccgtc gatgtagact tgaccgccat gggtatggac gatttggcaa atgcgccg
//
LOCUS       DATNDD010000302         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2291541, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000302 DATNDD010000000
VERSION     DATNDD010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291541"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VN647_07250"
     CDS             complement(<1..273)
                     /locus_tag="VN647_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWG96865.1"
                     /translation="MKNPFGNMNNILKQAQAMQEQMAKIQEQAASKTASGTAGGGIVT
                     VTANGAMQIVSVAIDPEIVKSGDVDMLQDLVVAATNEALRKAKELME"
     gene            complement(270..2063)
                     /gene="dnaX"
                     /locus_tag="VN647_07255"
     CDS             complement(270..2063)
                     /gene="dnaX"
                     /locus_tag="VN647_07255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWG96866.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQPHVVQTLMNAVSTKRIAHAYLFS
                     GTRGVGKTTVARILGKALNCERGPTSHPCNTCENCLEIMQGNSVDVIEIDGASNTSVD
                     DVREIRENVKFTPFRGQFRVYIIDEVHMLSNSAFNALLKTLEEPPTHVVFIFATTEIH
                     KIPATILSRCQHYNFRRIARAEIIERLRHVAAQDWLTLEERSFAALARASEGSMRDAL
                     SLLDQAVAYGGKTISHADLELLLGAVPQELVQELIRAIMAQDSPAALASLANLLDRGH
                     DLRAFCAEVVEHIRNLLVAAVVPGTVELRSLIEISEDDLNQLSMNAKTLTPEQLQEVL
                     AIFIQAEDALRFTNHPRFVMETAAVRATRLLRQREGIEARPVQTASSSTQKPPVEPEG
                     RKGGPSTLPALRQDVPVASRPSPKPTQTSRAETEGGRSISSEPSHDPPPDVATATPAA
                     VPSLAVDPQPTLHWELVQEEVAVSFPNIAPFLEAGRLVGMEDGIVTIGFAKQATVARA
                     RLEKEENLLVLSKLCERQLGYPIRVRITELTETHPPGPTMAQVRAAKEQEQRLVLFER
                     AKANPTVKQALEIFGVELAEVRTITQQEASE"
     gene            complement(2173..2272)
                     /gene="ffs"
                     /locus_tag="VN647_07260"
     ncRNA           complement(2173..2272)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN647_07260"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2381..2470)
                     /locus_tag="VN647_07265"
     tRNA            complement(2381..2470)
                     /locus_tag="VN647_07265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2434..2436),aa:Ser,seq:gga)
BASE COUNT          594 a          788 c          779 g          679 t
ORIGIN      
        1 ttccatcaat tccttggctt tgcggagcgc ttcgttcgtc gcggccacga caagatcttg
       61 gagcatatcg acatcgccgc tcttgacgat ctcggggtcg atcgccacgc tgacgatctg
      121 catcgccccg ttcgccgtga cggtgacgat cccgccgccg gctgtcccgc tggcggtttt
      181 tgacgccgct tgctcctgaa tcttggccat ctgttcttgc atggcctgag cttgcttgag
      241 tatgttattc atgttgccga aaggattttt cattcgcttg cctcctgctg ggttatagta
      301 cgaacctctg ccagttccac gccgaatatc tcgagggctt gcttcaccgt cgggttcgct
      361 ttcgcacgtt cgaacaacac cagccgttgt tcttgttcct tggccgctcg tacttgggcc
      421 atggtcggtc ccggtggatg agtctcagtc agctcggtga tgcggacacg tatgggatat
      481 cccaactgac gttcgcataa ttttgataac acgagaaggt tctcttcctt ctccaatcta
      541 gctctggcta cggttgcctg cttggcaaac ccgatggtga cgataccatc ctccatcccg
      601 acaagcctgc cggcttcgag aaacggcgcg atatttggaa atgagactgc aacctcttct
      661 tggaccaatt cccaatgcaa cgtcggttgt ggatcgacgg ctagagaagg cacggcggct
      721 ggggtggctg tcgccacatc aggtggggga tcgtgagatg gttcagaaga gatcgatcgc
      781 ccgccttctg tttccgccct agaggtttgc gtaggtttag gtgaaggcct tgaggcaacg
      841 ggtacatcct ggcgcaaagc cggaagagtc gatggtccac ccttgcgtcc ttccggctca
      901 acgggaggtt tctgggtgga tgacgacgct gtctgtaccg ggcgcgcctc tatgccctct
      961 cgttgacgca gcaaccgtgt ggctcgaaca gcagcggtct ccatcacaaa acgagggtgg
     1021 ttggtgaatc gtaaagcatc ttccgcttgg atgaagattg ccagcacctc ctgaagctgc
     1081 tccggagtca gtgtcttggc attcatcgac agctggttca gatcgtcttc ggagatttca
     1141 atcaagctcc gcagctcgac cgtaccggga acgacagccg ccacgagcaa attgcggatg
     1201 tgttccacga cttccgcaca gaacgcccgc aagtcatgcc cccggtccag cagattggcc
     1261 aggctggcaa gggcagcagg gctatcttgg gccataatcg ctctaatgag ttcctgcacc
     1321 agttcttgag gtactgctcc taacagcagt tcgagatccg cgtggctgat ggtcttgccg
     1381 ccatatgcaa cggcctgatc gagcaaactc aaggcatcgc gcatgcttcc ttcactggcg
     1441 cgggccaggg ccgcgaagct ccgttcctca agcgtcaacc aatcttgcgc tgctacgtgc
     1501 cgaagtcgct caatgatttc ggctctggca atgcgacgaa agttgtaatg ctggcatcgc
     1561 gagagaatcg tcgcggggat tttatgaatc tccgttgtgg caaaaatgaa aaccacatgc
     1621 gttgggggtt cttcgagtgt cttcaatagg gcattaaacg ccgagtttga gagcatgtgg
     1681 acttcatcga tgatgtaaac tcgaaactga ccgcgaaacg gcgtgaactt cacgttctcg
     1741 cggatctctc gcacatcgtc cacgctggtg ttggacgcac catcaatttc gatgacatcg
     1801 actgaattcc cttgcatgat ctcgaggcag ttctcgcagg tattgcaggg atggcttgtc
     1861 ggtccccgtt cacagttgag tgcctttccg agaattcgtg cgacggtcgt tttccccacg
     1921 cctcgtgtgc cggaaaatag atacgcgtgc gcgatccgtt tcgtcgagac cgcgttcatt
     1981 aacgtttgga cgacatgggg ctgaccgatc acatcgtcaa acgtaccggg ccgatattta
     2041 cgtgcggaga cttgatagtc cataaagaag ccttcaggag tgagagccga gatgtaagga
     2101 atgtaaaaca agacgatata aaaccgacga cgctgtcgcc ttacacctca tgctcacgcc
     2161 tcacgtccaa agtggggcca ggtgatcctg cggcacacag aactggctgc ttaccgttgc
     2221 ttccttccgg acctggcggg gttcacagga ttctattgca cagggcccag cccctattct
     2281 ccatctaggc tctccaatac agtccgcact taaggtatgt atccgcccgc agaaattacg
     2341 atacagcgat aaaagcgggc ggtactcacg aagtgcggta tggcggagag ggtgggattc
     2401 gaacccacgg tcccgttgcc gggacgcatg ctttccaagc atgtcgattc gtccactctc
     2461 gcacctctcc gcattgagaa gtgcaaagag gacgcatctt agcatgggag ttcatgacgg
     2521 gcaaggtgaa cagcgtctcc gttgtcatta agcctctaag cctcgcggag gcaattcctc
     2581 tatacaagac ggtcatcttg gtgtaccacg gcgaactgta ggcgaagttt tcggtgagca
     2641 aggatcgcca aacagctcgg ctgttccatt cgtaccaaga acaggccacc ctcggtcgta
     2701 tgggttgaca ttacaggtgt ccaacggtac aacgctatac gacatcgcgt gtttttgcag
     2761 tctcggtcac tctactacag ctagatctaa atagggtgac agctccgtgg cattccttac
     2821 ccatgcgaga gtggcggaac
//
LOCUS       DATNDD010000303         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_913773, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000303 DATNDD010000000
VERSION     DATNDD010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913773"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VN647_07270"
     CDS             complement(<1..613)
                     /locus_tag="VN647_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HWG96867.1"
                     /translation="MTKISIARLYTAETIAIGSELLVGGRSDGNSLFIADALAAIGIE
                     VRFKTIVGDDQSDIAGVLTTACRRAGIVIMTGGLGPTVDDCTREAVASVTGFRLARRK
                     EAFESMSARLAQWGRTPNRGQLRQALIPSGATVLTNSVGSAPGFRLTWRNAWVIALPG
                     VPSEMQAMMQQSVIPFLADRLGRSRQARPHPITRVTFHTWGLPE"
     gene            complement(617..1183)
                     /locus_tag="VN647_07275"
     CDS             complement(617..1183)
                     /locus_tag="VN647_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96868.1"
                     /translation="MVAVWTCGFACPSAAIEVQPAQIHIQLALDRGKEAAAHRRAPET
                     FYTRFGSDEDLHAGGFLVTKLGGLSVLATHMALRGLEPSAADIAQVTEASTMLVSTVI
                     LGDSPSFAVSSYIVLDQGGKVVKPATVRVDGLAGRSSTWPKSPKFRAKVVATFSYADF
                     DPNAQTTITVFPATGGEVHFSLNFADID"
     gene            1369..2091
                     /locus_tag="VN647_07280"
     CDS             1369..2091
                     /locus_tag="VN647_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HWG96869.1"
                     /translation="MSTPDTPAATPTPRRQYVNFVFYKVDPAWRRLPEDVRTQGKQEF
                     LRAVEDFNGKVLVVPYSTIGIRGDCDFMLWRISYDLDLFQDMGTKILASGLGQYLSTP
                     YSYLALTKRSVYVDHHSHAGQEGKRLTVVPGKSKYIFVYPFLKTREWFLLTKAARQGM
                     MDEHIEVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTL
                     RDTPIFTCIRRSLKETLDTLGG"
     gene            2256..2474
                     /locus_tag="VN647_07285"
     CDS             2256..2474
                     /locus_tag="VN647_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96870.1"
                     /translation="MADTTALYALRFPDGSVSLYIDEQYAQDRGIDPSRLVRVEIPRE
                     LFVSGTVQDIREYVALHFEQHQPHTGTA"
     gene            2598..>3225
                     /locus_tag="VN647_07290"
     CDS             2598..>3225
                     /locus_tag="VN647_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96871.1"
                     /translation="MVQALGTSSLTTEKIEEVIAGLPIQGRIILRLLLIQYLDVTHEE
                     ILYMVSDRPDPRCVSGKKPVTTMTQESITAMIERRNEYRRRVRLRREQTWLQCAALTN
                     QAHMANTLAIRAAALLAAHGVSSETIERLTAQARSAVPSTTLRLLEESWDKNEITLEE
                     YQKHRLAVEMQTQLRFAERFKKRLGLAQREQQTSDCTVLADHEIGHIWG"
BASE COUNT          759 a          920 c          849 g          697 t
ORIGIN      
        1 actccggtaa tccccaggta tgaaaggtca ctcgtgtgat gggatggggc cgtgcctgcc
       61 tcgatcggcc gagtcgatcc gccaagaacg gaatcaccga ttgctgcatc atggcctgca
      121 tctcacttgg gactcccggc agtgcgatca cccaggcgtt tcgccatgtc agcctaaagc
      181 caggtgcgga tccaaccgag ttggtcaata ctgtcgcccc tgacggaatc agggcttgcc
      241 gcaattgtcc gcgattcggt gttcgtcccc attgagccaa cctagcactc atgctctcga
      301 acgcttcctt acggcgagcc aggcggaatc ccgttaccga cgcgacggct tctctggtgc
      361 agtcgtcgac cgtcggacct aagcccccgg tcatgatgac aatcccggcc cgacgacagg
      421 ctgtcgtcag cactccggca atgtccgact gatcatctcc cacgattgtc ttgaacctga
      481 cctcgatacc gatggcggcc aaggcatcgg cgataaacag tgaattgccg tcggaccggc
      541 ctcccacaag cagctcggaa ccaatcgcaa tcgtttccgc agtatacagg cgtgctattg
      601 atatctttgt catcgactaa tcgatatcag caaagttgag tgagaagtga acctctccgc
      661 cggtcgcagg aaagactgtg atggtcgtct gcgcattcgg atcgaaatcg gcatagctga
      721 aggtggctac gactttcgca cgaaattttg gtgacttggg ccatgtcgaa ctccgaccgg
      781 ctagcccatc gacccgcaca gtggcaggct tgaccacctt tccgccttga tcaagcacga
      841 tataactgct tacagcaaat gaaggactat cccctaggat caccgtactc acaagcatcg
      901 tcgaggcctc agtcacttga gcaatatcgg cagcactcgg ttcaagccct cgtaaggcca
      961 tgtgtgttgc caggaccgac aatcccccga gtttcgtgac aagaaatccg ccggcatgca
     1021 gatcttcgtc acttccgaat cgagtataaa acgtctcagg agctcggcga tgcgccgctg
     1081 cctctttccc tcgatcgagt gccagctgga tatgtatttg tgccggctgc acttcaatcg
     1141 cggcagacgg gcacgcgaac ccgcatgtcc agactgccac catccacacg gacccgtacg
     1201 caggagattt cataggactg cgagtaacag aaccctggtg gcctgtcaat gaatgctctt
     1261 tcgaatactc tttctgcctt gctctccggt tgacaagact gaaggggaaa tgctaaacga
     1321 agaaaaaatg aggaactgtt tcattttaat ttactaaagg agttttgaat gtcgaccccg
     1381 gatactcctg ctgccacccc gacgcctcgg cgccagtatg taaactttgt tttttataaa
     1441 gtggaccctg cctggcggcg cctaccggaa gatgtgcgca ctcaaggcaa acaagagttt
     1501 ctccgggcag tggaagattt caacggcaaa gtgctggtag tcccctattc caccatcggc
     1561 atccgggggg actgcgactt catgctgtgg cgtatcagct acgacctcga cctgttccag
     1621 gatatgggca ccaagatcct cgcctccggc ctcgggcagt acctctccac cccatattct
     1681 tatttggcgc tgaccaaacg gtccgtctat gtcgaccacc actcacacgc aggacaggaa
     1741 gggaaacgcc tgaccgtggt gccgggaaag agcaagtaca tctttgtgta cccctttctc
     1801 aagacacgag agtggtttct gctgacgaag gccgcgcgtc agggcatgat ggacgaacac
     1861 atcgaagtgg gacatcggtt tccatccgtg aagctgaaca cgacgtattc gttcggtttg
     1921 gacgatcagg aatgggtcgt ggcgtttgaa agcgacaagc cggaggattt tctcgacttg
     1981 gtaatggcgc tgcgggagac ggagggatcg cggtataccc tgcgggacac tccgattttc
     2041 acctgcatcc gccgcagcct taaagaaacg ctcgacacgc tcggcggcta gatatcgaat
     2101 caaagtagta cgagggtctt cggttgttat gaccgaagac cctttgtttt tatcgacaat
     2161 gaactcatcg ctaacacccc taccgcgcgt ctctgcggcc tttgatagtc tggcgaggcc
     2221 tgtgttacct ctacaaggtc atcgctggag tatcaatggc tgacaccacc gctctctacg
     2281 ccctgcgctt tcccgatggc tcggtgagcc tgtacatcga tgaacaatat gcccaagatc
     2341 gaggtattga tccttcccgg cttgtgcggg tagaaatccc acgagaattg ttcgtcagcg
     2401 gcaccgtaca ggacatccgt gaatatgttg cgcttcactt tgaacaacat caaccacata
     2461 ccggaacagc atagccggtg cttaagaaat cttgagaagt ggaggctcgg gttaaggctg
     2521 aggcgaaaga aggatggaca gagattcatc tttgttcaac cgaaacctaa cccccttaac
     2581 ctgctcataa cgacatcatg gtacaagcac tgggaacatc gtcgctgaca accgagaaga
     2641 tcgaggaagt gatcgccggc ctgccgatcc aaggccgcat catcctcagg ctcctgctga
     2701 ttcagtacct ggacgtcacc cacgaggaaa tcctctatat ggtgtcggac agaccggacc
     2761 ctcgatgtgt gtccggcaag aaaccggtca cgacgatgac acaagaatcg atcaccgcga
     2821 tgatcgagcg ccggaatgaa taccggcggc gtgtgcgact tcgccgagaa caaacatggc
     2881 ttcagtgtgc cgcactcacc aatcaggctc acatggcgaa cacgttggcc atccgggctg
     2941 ccgcattgct ggctgcgcat ggagtgtcct cagaaaccat cgaacggctc acagcgcagg
     3001 cccgttccgc tgttccctcg acgacattgc ggctactgga ggaaagctgg gataaaaatg
     3061 aaatcactct tgaggaatat cagaagcatc ggctagccgt tgagatgcag acgcaacttc
     3121 gctttgccga acggttcaag aaacgcttgg gcctggcgca acgtgagcaa caaacatcgg
     3181 attgtaccgt cttggccgat catgaaatcg gccatatctg gggaa
//
LOCUS       DATNDD010000304         4398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_608908, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000304 DATNDD010000000
VERSION     DATNDD010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608908"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VN647_07295"
     CDS             <1..541
                     /locus_tag="VN647_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWG96872.1"
                     /translation="AFDGTFSRIVCIGLLEFSDQLEARSAVAWFGANERELLRQFWAR
                     LAQDRPALFITHNGLGFDLPFIKKRSIINQVKPSLDINLAKFRTEPVYDTMAIWSNWD
                     MRGWVKLDVLARALQVDTKSGSGEHVAEMWERRQGRELAQYCLQDTYVTYACYCRMNF
                     RQPLSREVVLLQPELYDLG"
     gene            complement(554..1552)
                     /locus_tag="VN647_07300"
     CDS             complement(554..1552)
                     /locus_tag="VN647_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96873.1"
                     /translation="MHAYFNVRGFRYIGALMSLSVVVTGCSMLSGEAKLHKNAKGSVY
                     LKEIADWSFEAGHPATIDQMTMLKIVKGLVTDDLQNMSGNMPASGSKPMRVFSDEDAE
                     FLAPLLAQGLSQAKPEQIVGFKVSPSAGSGTEPTAGTLYMHHGSIYLTIAPSQTRKTA
                     GFTPSSVARLEKTPSFVALGSGAMTMVIDYQALAKGPMPSAPHAAGASKPMPVSTSQS
                     APVAPTALPAPSASPVKIAPPQEGSIGDPRTDSGTPQLSTDELLNRKLDELREAREAN
                     KLKDSEIAMLKKETTWMKKELRQRTEEVKTLKTSKTAVRSAPSAPRKKSAEAYPTR"
     gene            complement(1592..1792)
                     /locus_tag="VN647_07305"
     CDS             complement(1592..1792)
                     /locus_tag="VN647_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96874.1"
                     /translation="MKLAYVSASICSATRNSLLVIMKSADPESKPNPNPSDSGITLET
                     IISVGVVGWLAIGMVLMALGIW"
     gene            complement(2167..3498)
                     /gene="miaB"
                     /locus_tag="VN647_07310"
     CDS             complement(2167..3498)
                     /gene="miaB"
                     /locus_tag="VN647_07310"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWG96875.1"
                     /translation="MRVKSFPHVHIETFGCQMNESDSELVRSMLKREGFVFTEDRERA
                     DVVLVNTCAIRENAHNKIYAHLSELKALKKQRPLVVGVLGCMAQNLKSALAEKEPLID
                     VLAGPDAYRQLPLLLTTALESQEQGLPQKGFALDLSEYETYEGIMPDRDSGVNAWITV
                     MRGCDNFCSFCVVPYTRGRERSRDPKSILLEARDASAREFKQITLLGQNVNSYRHEHW
                     DFAKLITAVADTAGIDRVRFTSPHPKDFPPALIEAIAAHPKICKHVHLPLQAGSDRIL
                     ELMGRTYTGAEYLTLADRIRSTVPDVVLTTDIICGFCSESDEDLQATHRMVEQVQFDS
                     AYIFKYSERKHTIAARKYVDDVPDHVKGMRVAQLVETQRRITLERNRRYIGKDMEILV
                     EGDATRSSTQTMGKTDGNITVVWDKSTGDFKSGTLITKRIFDASAATLYGE"
     gene            complement(3495..>4398)
                     /locus_tag="VN647_07315"
     CDS             complement(3495..>4398)
                     /locus_tag="VN647_07315"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HWG96876.1"
                     /translation="STRALLKVHDGCSAMCSFCIIPFARGHERSRTLEDIQREVEVLA
                     ARGYREIVLTGVNIGQYAHQGRDFCALLRWLNQAADFERIRISSIEPTTVGEELLDLL
                     ASSKKLCPYLHIPLQSGDDQILQVMNRRHTIKSYIKLIERAFVTIPDLGLGTDLLVGF
                     PGETETAFQNTLAVATDLPFSYIHVFPYSSRPGTAAMRLKQRISSASVKKRTDLLLSL
                     DRAKRLAFHNKQIGKTVSVLFEAGTRDAYRFGTTPNFTRVGITDSGDFQNRILPVTIT
                     AATDRCAFGHVASAQPTPSAMAVI"
BASE COUNT          973 a         1089 c         1269 g         1067 t
ORIGIN      
        1 ggcgttcgat ggaacgttca gccgcatcgt ctgtatcggc ctactggaat tttccgatca
       61 gctggaagca cgcagtgccg tcgcatggtt cggagcgaac gagcgtgagc tgctccgcca
      121 attttgggcc agactagccc aggaccgacc tgccttgttc atcacgcata acgggcttgg
      181 cttcgatctt cccttcatca agaagcggtc gatcatcaat caagtgaaac ccagcctcga
      241 catcaatcta gcaaaattcc gaaccgaacc ggtctatgac acgatggcca tctggagtaa
      301 ttgggatatg agaggttggg tcaagctgga tgtattggcc cgtgcattac aggtcgatac
      361 gaagtcgggc agtggagaac acgttgctga aatgtgggaa aggaggcaag gtcgtgagct
      421 tgcacagtac tgtctgcaag acacctatgt aacttatgcc tgctattgcc gcatgaactt
      481 tcgtcagcct ctgtccaggg aggttgttct gttgcagcct gagttgtacg atctcggttg
      541 attgatcggt cgatcaacgg gtcggatacg cttccgccga tttttttctc ggggctgacg
      601 gggctgaacg gacggctgtc ttactggtct tcaaggtttt cacctcttcc gtccgttgac
      661 ggagctcttt cttcatccac gttgtttctt tcttcagcat cgcaatctca gagtccttaa
      721 gcttgttggc ctcgcgcgct tcccggagct catcgagttt cctgttgagc agttcatcag
      781 tgctcaattg aggagttcct gaatcagtcc gagggtcccc gatagaacct tcctgtggcg
      841 gtgcaatttt cacaggtgaa gcgctcggtg caggcaacgc agttggtgcg actggagcgg
      901 actgagacgt ggaaaccggc atgggctttg aggctccggc tgcatggggt gcgctcggca
      961 tcggtccttt ggcaagggct tggtagtcga tcaccatcgt catcgctccg cttcccagcg
     1021 caacgaaaga aggagttttt tcaagacgag cgacagaact gggagtgaat cccgcggtct
     1081 tcctggtttg agaaggagcg atggtcagat agatcgatcc atgatgcata tagagggtgc
     1141 ccgctgtggg ttcagtgcct gaccctgccg atggagacac cttgaaaccg acaatctgct
     1201 ccggtttcgc ttgcgataag ccttgggcta gcaggggagc aagaaactcg gcgtcttcat
     1261 cgctgaatac tctcatcggc ttgctgccac tggcgggcat gttgcctgac atgttctgaa
     1321 ggtcgtcggt cacaaggcct ttgactattt tcagcatcgt catctgatca atggtggctg
     1381 ggtggccggc ttcaaatgac caatcggcga tctccttgag atagacggag cctttggcat
     1441 ttttatggag ctttgcctct ccggaaagca tcgagcatcc ggtcacgacc acgctgaggg
     1501 acatcaacgc accgatatat cggaatccgc ggacattgaa atatgcatgc atatgggatt
     1561 tcctcacatg gtcttgtgat cggatgtcga cctaccagat acctagcgcc atgagtacca
     1621 tgccgatcgc caaccatccg actactccga ccgaaatgat tgtttcgagt gtgatgccgg
     1681 agtcggacgg gtttggattg ggctttgact cggggtcggc ggacttcata ataacgagaa
     1741 gcgaattcct cgttgccgaa cagatggacg ccgaaacata agcaagcttc atgccttctc
     1801 atttttgaga taatgggttg tattgtcatt cagtgcgaag gagacacgcc tgataaaaac
     1861 tcaaagttag aagttgagga ctgatgaccg agctcagaag cgaggtcttg gggtgctttg
     1921 ataatcattg ggttagtgtc gccaaatgat cgaacagctg ggaagattcg atcgatcggc
     1981 aacgagtctg gccataaacg aggccagatg ggaagcgaag acagggaaag tacaaccgaa
     2041 ggaccgtccg tggatagcca gtggagtcgg tcaaaaaatg ttcatttcga ccgttgcacg
     2101 tgcagaattg ttccgaaagc ttgagacagg tgtctcaaaa agaatacgtc ctcaggactt
     2161 gtcaggtcat tcgccataaa gggttgcggc cgaggcatca aagatacgtt ttgtgatcaa
     2221 cgtgcctgat ttaaaatcac cggtgctctt atcccatacg accgtgatat ttccgtcggt
     2281 tttccccatt gtttgggttg atgagcgggt cgcatcacct tcgactaaga tttccatgtc
     2341 tttgccgatg tatcgtcggt tgcgctccaa ggtgatgcgg cgctgagttt ctacgagttg
     2401 agcgacgcgc atacctttga cgtgatcggg gacatcgtcg acatactttc tagctgcgat
     2461 cgtgtgtttc cgctccgaat atttgaagat gtaggctgag tcaaattgga cctgttccac
     2521 catccggtga gtggcttgta agtcctcatc cgactcggag cagaaaccac agatgatatc
     2581 cgtagtcagg acgacgtcgg gaacggtgct tcgaatgcga tcggccaatg tcaggtactc
     2641 ggctccggta taggttcggc ccatcagttc aaggatacga tcgctccctg cctgaagggg
     2701 aagatgaacg tgcttgcaga tttttgggtg tgcggcaatg gcctctatga gagcgggggg
     2761 aaagtcttta gggtgcggag aggtaaaccg tacgcgatcg atccctgctg tatccgccac
     2821 ggcggtgatc agtttggcga agtcccagtg ttcatggcgg taggaattga cgttctggcc
     2881 gagcaaggtg atttgcttga actctcgggc ggaagcgtca cgggcttcaa gcagaatgga
     2941 tttcggatcc cgcgaccgtt cacggcctct ggtatagggg acgacacaga acgagcagaa
     3001 attatcacac ccccgcatca cggtaatcca ggcgtttacc ccgctgtcgc ggtccggcat
     3061 gattccttca taggtttcat actccgacag atcgagggca aaccctttct gggggagtcc
     3121 ctgttcttgg gattcgagcg ccgtcgtcag caacaagggg agttggcggt aggcatccgg
     3181 gcccgccaag acgtcgatga gcggctcttt ttccgccagg gcgcttttca gattctgggc
     3241 catacagccg aggacgccga cgacgagcgg gcgctgtttt ttcaatgctt tcaattcaga
     3301 aagatgggcg tagatcttat tgtgcgcatt ctctctgatg gcgcaggtgt tgaccaatac
     3361 gacgtcggcc cgttcgcggt cttccgtgaa aacgaatcct tcccgcttca gcatcgagcg
     3421 aacgagttcg ctgtcggact cattcatttg acagccgaag gtttcaatgt gaacgtgagg
     3481 aaaagatttc actctcatat caccgccatt gcagaaggcg tgggctgcgc cgaggcgaca
     3541 tggccaaaag cacagcggtc agtcgccgct gtgatcgtga cgggcaggat ccgattctgg
     3601 aaatctccgg agtctgtgat gcccactcgt gtaaagttcg gagtcgtgcc gaaacgatag
     3661 gcatcccgag tgccggcttc aaaaaggacc gagaccgtct tgccgatttg tttgttgtga
     3721 aaggcgagtc gcttggctcg gtcgaggctc aggaggagat cggtgcgttt ctttaccgat
     3781 gccgacgaga tccgctgctt cagacgcatt gcagccgtcc caggacgaga ggagtacgga
     3841 aatacgtgga tataggagaa cggaagatca gttgcgacgg ccaacgtatt ctgaaaagcg
     3901 gtctcagtct ccccggggaa tcctaccagg agatcagttc caaggccgag gtcggggatg
     3961 gttacgaaag ccctttcgat cagtttgata tagctcttga tcgtatggcg tcgattcatg
     4021 acctgcaaaa tctgatcgtc cccgctttgc aacgggatgt ggagataggg acagagtttc
     4081 ttcgacgagg cgaggagatc aagcaattcc tcgccgactg ttgtgggttc aattgaagag
     4141 atgcggatgc gttcgaagtc ggccgcttgg ttgagccaac gaagcaatgc acaaaagtcg
     4201 cggccttggt gcgcatactg gccgatgttc actccggtca gcacgatttc ccgatatcct
     4261 cgagctgcta agacttcaac ttcacgctgg atgtcctcaa gggttctgct tcgctcatga
     4321 ccccgtgcaa acggaatgat gcagaaactg cacatcgcgc tgcagccgtc atgtactttg
     4381 agcaatgcac gggtggag
//
LOCUS       DATNDD010000305         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2789087, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000305 DATNDD010000000
VERSION     DATNDD010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789087"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VN647_07320"
     CDS             complement(<1..897)
                     /locus_tag="VN647_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWG96877.1"
                     /translation="MISSSHQIKPAPETHLQRTLNAALNDIGTDAALAAIFHQENGPL
                     VEHASRGFTPRDVQAILRTLSTQRAAALVPTTQDPDGGRVIRLRLITPGAKSLLVVPL
                     RHFNSVYGCLVIGRKEGTAFSKKDKSHLEQMCDGMTKALDREGLFNTNAVLSRSYVTQ
                     EPVLSQQTAADLFPPMTKHFSPELQEKIETVLTEADQYVTYDRAWACYYDPLAGNVEV
                     LGIVGDVKADPKDAKKDLKPGQRLTLDSSAAGWAVRHRKPRVDHDLASTQGRFLDHKH
                     LFKDRFQSSLVIPFFVKGQVGGT"
     gene            complement(1095..1586)
                     /locus_tag="VN647_07325"
     CDS             complement(1095..1586)
                     /locus_tag="VN647_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96878.1"
                     /translation="MVHRFRHILFVAVLLGLFVQIAWSDERPAPKSLWQTVTAPPPTG
                     QPSIPSKPWVLREREIVIDLPLLHVLKDAGARPLPRITVELFDKPNLELDVISTVSRI
                     NGTAVVRGTFKPPSVGDFTFVVTGNLLTGTIQMGNRFYKIEHIANGRLQLVEIDPGKL
                     PLD"
     gene            complement(1723..2181)
                     /locus_tag="VN647_07330"
     CDS             complement(1723..2181)
                     /locus_tag="VN647_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWG96879.1"
                     /translation="MPTIRFARAVHESPFHGGVRHALTRVLITGIAVFLAIAIIPGLE
                     ADSLTAGIAAVLVLTFLNVILRPILFLLTLPLIVFTLGLFLVVLNALLLEFTSFLVKG
                     FTVSGFWPAVGGALIISLVTTILNSWTVDRRPLPDFQDEPRRPPKIINPD"
     gene            complement(2185..2529)
                     /locus_tag="VN647_07335"
     CDS             complement(2185..2529)
                     /locus_tag="VN647_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96880.1"
                     /translation="MLKNYDDRARGCLIFVAACVLPTIVMAAGTDLHVLGIVTAIDAK
                     HLDVKTAKGPVVSVLLNKQVKFKNKSNQKSNDPPVVGDRVIIEATKDDKTKKVSATVV
                     HYSPVKTASPPQ"
BASE COUNT          561 a          668 c          801 g          599 t
ORIGIN      
        1 cgtcccgccg acctggcctt tgacaaagaa gggaatgacg agcgacgact gaaaccgatc
       61 cttgaagaga tgtttgtggt cgagaaagcg gccttgggtg gacgccagat cgtgatccac
      121 gcgaggcttg cgatgacgga cggcccatcc cgctgccgaa ctgtcgagcg tcaggcgctg
      181 accgggtttt aaatccttct tggcgtcttt tggatctgct ttcacgtcac cgacgattcc
      241 caagacttcg acgtttcccg ccagcggatc gtaatagcag gcccaggcgc ggtcatacgt
      301 cacgtattgg tcagcttcag tcaagaccgt ctcgatcttt tcttggagct cgggtgaaaa
      361 atgcttggtc atcgggggaa acaggtccgc cgcggtttgc tgggaaagga cgggctcctg
      421 tgtgacgtag gaacgactca ataccgcatt ggtattgaag agtccttcgc ggtccagcgc
      481 cttagtcatg ccgtcgcaca tctgctccag gtgagatttg tccttcttgg agaaggctgt
      541 gccctctttc cgtccgatga ccaggcaacc atagacgcta ttgaagtggc gaagcggaac
      601 gacgagcaag gatttcgctc cgggggtgat caaccgcagg cggatgacgc gcccaccatc
      661 cggatcttgg gtggtcggga ccaaggctgc ggcacgttgc gtcgataggg tacgaagaat
      721 ggcttggaca tctctcggtg tgaacccgcg cgatgcatgc tcgacgaggg ggccgttttc
      781 ttgatggaag atggccgcca atgcggcatc cgtgccgatg tcgttcaatg cggcgttcaa
      841 cgtccgttga agatgtgtct cgggggcggg ttttatttga tgacttgaac tgatcatacg
      901 ttcacgtacg agcaaaggca ttgtagcggg aatgccgatg aatatctact tcctccggac
      961 tgttttcgat acgttggcgt gagctgccac aacgtttgca cggcgaagcc ggtcggcttc
     1021 ttctaaccgg tgatgatcga gcagcagttc gagcattttt tacttggtcg gcggtcagtg
     1081 cgaggttgtt gcgatcaatc cagcggcagt ttgccgggat cgatttcgac cagttgtagc
     1141 cgaccattgg caatgtgctc aattttatag aaccgattcc ccatctggat agtccctgtc
     1201 aggagattgc cggtgaccac aaaagtaaag tcacccacgg agggtggttt gaaggtgccg
     1261 cggacgacgg cggtgccgtt gatacgagat accgtggaga tgacatccaa ttcgaggtta
     1321 ggtttatcga agagctctac ggtgatcctg ggaagaggcc tggctccagc atccttcagc
     1381 acgtgcagga gcgggagatc gattacgatc tctcgctcgc ggagaaccca cggcttgctc
     1441 ggtatcgatg gttgaccggt gggaggagga gccgtaacgg tctgccatag actctttggt
     1501 gccgggcgtt catccgacca tgcgatctga acaaagagac cgagcagcac cgcaacgaag
     1561 agaatatgcc tgaatcggtg aaccatggaa tcgtcccctg cttcccagcc ccctatgatg
     1621 ctcctgataa tttgagcata actggggaga tccttccatt ggcgtaactg aaagagaagc
     1681 tcgttctgtc aatcgcgaat tcctcgtgaa ttgtcgaatg cttcaatccg gattgatgat
     1741 tttgggcgga cggcgcggtt cgtcctgaaa atctgggagg gggcggcggt cgacggtcca
     1801 gctattgagg atggtcgtaa ccaggctgat gatgagcgcg ccaccgacgg cgggccagaa
     1861 tcctgagacg gtgaaccctt tcaccaggaa tgacgtgaat tccaacagca gcgcgttcag
     1921 gaccacgagg aagagtccga gcgtaaacac gatcagcggc aaggtgagca ggaagaggat
     1981 agggcgaagg atgacgttca agaaggtgag gaccaggacc gcggcaatgc cggccgtcag
     2041 gctgtcggcc tccaggccgg ggataatcgc gattgccagg aataccgcga ttccagtgat
     2101 gaggacgcgc gtgagagcat ggcgcacccc tccgtgaaac ggtgactcgt gtacggccct
     2161 cgcaaagcga atcgtcggca tcggctactg aggtggtgac gcggtcttta cgggtgaata
     2221 atgaaccacc gttgcggaca ccttcttggt tttgtcgtct ttcgtcgcct caatgatgac
     2281 acggtcgccc acgaccggtg gatcgttcga cttctggtta cttttgttct tgaacttcac
     2341 ttgtttattg agcaggaccg aaacaaccgg tcctttggct gttttgacat cgaggtgttt
     2401 tgcatcgatg gccgtcacga tgcccaacac atgaagatct gtgccggcag ccatgacgat
     2461 agtgggcaac acacaggctg ccacgaagat caggcagcct cttgcccggt catcatagtt
     2521 ctttaacata gagacgtcta gtgatggtgc ttgtcgtctt tgtcatggtg aggcgccttg
     2581 gggttcaacg actgcatgcg ctccagttcc tcgtgggtca agtgaggca
//
LOCUS       DATNDD010000306         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_376137, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000306 DATNDD010000000
VERSION     DATNDD010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376137"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN647_07340"
     CDS             complement(<1..249)
                     /locus_tag="VN647_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96881.1"
                     /translation="MDRKQTVPVHRGFVSCFILVSTVTSALVFAFLAHALDVPPLTGR
                     IVDLAHVLPNSTMESLTAQLAAHETQSSNQVAVLIVPSL"
     gene            complement(283..885)
                     /locus_tag="VN647_07345"
     CDS             complement(283..885)
                     /locus_tag="VN647_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWG96882.1"
                     /translation="MGRTWWKGILITVTMISALSVSGCGYNDLQGLDEDTKAAWSEVI
                     NQYQRRADLIPNLVATVKGYAAHEKETLEGVVNARAKATGMQVTPEVLKDPAAFEAFQ
                     KAQAGLTSALGRLIAIAENYPNLKADQNFRDLQSQLEGTENRITVARKRYIDRVAEYN
                     KMVRFFPTNLTAKLLLHLEERPNFTVADENAVAKPPEVKF"
     gene            987..1292
                     /locus_tag="VN647_07350"
     CDS             987..1292
                     /locus_tag="VN647_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96883.1"
                     /translation="MPREPSIHKPGGFQVRHRTLGIYQGSAIDLAFWHPSSGMPEHGL
                     CRFLTRDKAQVLIDVLSSSACMEPMNRNDLMIEPFDVAEHDRLMTDYPLSSAWETPT"
     gene            complement(1308..1781)
                     /locus_tag="VN647_07355"
     CDS             complement(1308..1781)
                     /locus_tag="VN647_07355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG96884.1"
                     /translation="MRPQSPRELIAALIWIVPYRCGYCCHRFLARRLSVAGPPPPMER
                     REHLRIPVRLCLSFSGGKVRGEGIVMDLSLGGCIIKSETQVHVDDIFYLEIVVAEDEP
                     PVEVAAMVRSVSARGIAFKFLRKAQENKRLLTFIQSHSGSTSSVLSKAVEPTVTG"
BASE COUNT          483 a          601 c          599 g          484 t
ORIGIN      
        1 gagggaggga acgatcagga cggccacttg attgctcgac tgagtttcat gggccgccag
       61 ttgggccgtc agcgactcca tcgtggagtt cggcaagaca tgggcgagat cgacaatccg
      121 ccccgtcaac ggcgggacat cgagcgcatg tgcgagaaaa gcgaagacca aggccgaggt
      181 cacggttgag actaaaatga aacacgagac aaaccctcgg tgaactggga ctgtttgctt
      241 ccgatccaca gtctcaacct cgacctgaac ctcaacctgt acttagaatt tcacttcggg
      301 cggcttcgcc acggcattct catcggcgac cgtaaagttg gggcgctctt ccaggtgcag
      361 cagcagcttc gctgtaaggt tggtcgggaa aaatcggacc atcttgttat attccgccac
      421 tcggtcaatg taccgcttac gcgctacggt gatccggttt tccgtcccct ccagctggct
      481 ttggaggtcc ctgaaatttt gatcggcttt caggttgggg taattttcgg cgatggcgat
      541 gagccggcct agcgccgatg tcagaccggc ctgggccttt tgaaatgctt cgaatgcggc
      601 tggatctttg aggacctcgg gggtcacttg catccctgtc gccttagctc tggcgttcac
      661 gacaccttca agggtttctt tttcatgagc cgcgtaccct ttgaccgtcg ccacgaggtt
      721 cggaatgaga tcggcacggc gctggtattg attgatcact tcgctccacg cagccttcgt
      781 atcttcgtcc aagccttgaa gatcgttata accgcaaccg gacacagaca atgcactgat
      841 cattgttacg gtgatcagaa tgcctttcca ccaagtacgc cccatcgttc ccctccttcg
      901 acagtcgagc agaacgcagt gtccctacat ctcgcggtca tgctaccatg ggcataatca
      961 ttaagatatg tcggtcaaga gggcctatgc cgcgggagcc gtcgattcat aaaccaggtg
     1021 ggtttcaagt acgacaccgc accctcggta tctatcaagg cagcgcgatc gacctcgctt
     1081 tctggcaccc gtcgtcaggc atgcccgaac atgggctctg ccgatttttg acaagagaca
     1141 aggcacaagt gttgatcgat gtgctctcct cttcagcctg catggaaccg atgaatcgaa
     1201 acgatctgat gatcgagccg ttcgacgtcg cagagcatga ccgcttgatg actgactatc
     1261 ctctgtcgtc tgcctgggag acgcctacct aggccggaag atcgagacta gccggtgaca
     1321 gtcggctcga cggctttgga gaggacagaa gaagtggagc ccgaatgtga ttggatgaag
     1381 gtcaaaaggc gtttattctc ttgggccttg cggagaaact tgaacgcgat gccgcgtgca
     1441 ctgactgacc gcaccatggc agcgacttcg accggaggtt catcctcggc gacgacgatc
     1501 tccaggtaaa aaatgtcgtc aacgtgcact tgagtttcac tcttgatgat gcaaccaccc
     1561 aaagagaggt ccatcacgat tccctcgcct ctgactttcc cacctgaaaa ggatagacaa
     1621 agccgtactg ggatacgaag atgttcacgg cgctccatgg gaggcggagg tccggcgacg
     1681 ctgaggcgac gggcgagaaa ccgatggcaa cagtatccac aacgatacgg aacgatccaa
     1741 atcaatgccg cgatcagttc gcgcggcgat tgtggccgca cacgaagcgc tccatctttt
     1801 cgacatcggg ggcagatcat ttgacgattc attcgatctc atctccggcc gctggagaac
     1861 ttttgctgca tccttatcgc actataaagg cctttccttc acagtcaagc tgattgccat
     1921 ctggcgcaaa tcagcctcca tcaacctttt acaccatggg aaggtcggcg gctgacgctc
     1981 ggcatcgcgt ttggttacga atcgaaacga gagaagaccg tgtcgtcggt ctgtccgcta
     2041 tgcggactct gcatgaggag attccggctc agcggccaga ccggattcga cgacgccggc
     2101 tcggagatat tcgcgattga gagtggcgat gaaacggacg ctgatgcgtt tcggacagac
     2161 ggcttca
//
LOCUS       DATNDD010000307         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2928139, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000307 DATNDD010000000
VERSION     DATNDD010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928139"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VN647_07360"
     CDS             <1..326
                     /locus_tag="VN647_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HWG96885.1"
                     /translation="MNLDEIRRKVQLRRYRISWTHTEKLRHRQISSDMIERAIETGVL
                     IESYPNDPRGPSCLLLGYSLEDRPLHVVCGKFDDDELLIITAYEPSAAEWKSDWRTRK
                     GGDEL"
     gene            323..556
                     /locus_tag="VN647_07365"
     CDS             323..556
                     /locus_tag="VN647_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HWG96886.1"
                     /translation="MTTCYFCKGRVSTQLTSVDFRWGEDLKIIEQVPAEVCEQCGEKY
                     FQAQVYKDLERLVQHPSRAIQHRSVDVLQYSAA"
     gene            complement(613..1356)
                     /locus_tag="VN647_07370"
     CDS             complement(613..1356)
                     /locus_tag="VN647_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWG96887.1"
                     /translation="MPQLHVNYVRTEEKLRTWSSADSLTANRSRPYDPSLMSNRHEQG
                     QINPALLVVLIVLIVTAVWVWKRLSVDTQDYIVDQAVPMAAIAVALAILLFVSVRALR
                     RRSTRTRERTRLLTLLEHESAWDKRLELAFALLELNEYRIDGLESAVPALKELFATTL
                     QRALDDKQHHIRGMAASHLGALQDMSVVPLLVKALEDDHAYVRSCAALGLGRLRATET
                     RDRLKNVMEQDWDQTVRSRAREALERMGG"
     gene            1442..>2267
                     /locus_tag="VN647_07375"
     CDS             1442..>2267
                     /locus_tag="VN647_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional universal stress/protein kinase
                     family protein"
                     /protein_id="HWG96888.1"
                     /translation="MVGTDRSKTADHAVQWAAEFADRYGAELFVVQVIVPQHPTTTEY
                     GAAEQTRAAAANDELAHVVGRLAGERGHAFVAIDSDPASAIVRAAEREAIDVLIVGNS
                     GMAGRKEFLLGNVPNRISHNSHCTVIIVNTLPSAGEPIPESGRMFQPQGEPHTPEPHL
                     GGRAVYIATVTAKHGLKELFSQPDQSDASTRRQQAKRLRAALEELGPTFSKLGQVLST
                     RPDLLPPEYIEELTLLQSHVPPMPESEVVRVSEQELRVPWEDVFESIDPKPLAAGTI"
BASE COUNT          501 a          628 c          636 g          502 t
ORIGIN      
        1 gaatgaatct tgatgaaatt cggcgaaagg tacagttacg acggtaccgt atttcatgga
       61 cacatacaga gaagctcagg catcgacaaa tcagtagcga catgatcgaa cgtgcaatcg
      121 agaccggcgt tctgattgaa tcgtatccca atgatccaag aggaccctcg tgtttgctct
      181 tgggatattc cttggaagac cgtccgcttc atgtggtctg cggcaaattc gatgacgatg
      241 agctcttgat tattaccgcc tatgaaccct ctgccgccga gtggaagtcc gattggcgaa
      301 cacggaaagg aggcgacgag ctatgacaac gtgttacttc tgtaaaggca gagtgagcac
      361 gcagttgacg agcgtggatt tccgatgggg cgaggacctc aagatcattg aacaggtgcc
      421 ggcagaagtc tgtgagcaat gtggtgagaa atacttccag gcgcaggtgt ataaagatct
      481 ggagcggtta gtgcaacatc catccagggc cattcaacat cgttctgtcg atgtgctcca
      541 gtattcagca gcctgatttc tgaggccatt gtgacggttc cacctcatct ccatccatga
      601 agcctctggc ttctacccgc ccatccgttc cagtgcctcc ctggctctgc tcctgacggt
      661 ttggtcccaa tcctgttcca tgacgttctt cagccggtca cgcgtttccg ttgctcgcag
      721 tcgccccaag cccaaggctg cgcaggaacg cacgtaggca tggtcatctt ccagcgcctt
      781 gaccaatagc gggaccaccg acatgtcttg taacgcgccg agatgactgg cggccattcc
      841 tctgatgtga tgctgtttgt cgtccagcgc tcgttgtaag gtcgtggcaa acagctcctt
      901 caaagcaggg acggctgatt cgagcccatc gattcggtac tcgttgagtt caagcaaggc
      961 aaaagcaagc tcaagccttt tatcccaggc agattcgtgc tcgagcaggg tcaggagcct
     1021 ggttcgttcc cgcgttctcg tactgcggcg acggagcgct ctcactgata cgaataataa
     1081 gatagcaagc gccacggcga ttgccgccat tggtactgcc tgatcgacga tgtagtcctg
     1141 tgtgtcaacc gacagccgtt tccacaccca taccgcggtc acgataagca cgatcagaac
     1201 gacaagcaag gcagggttga tctgcccttg ttcgtgacgg ttcgacataa gtgaaggatc
     1261 atacggacgc gatcgattgg ccgtcaacga atcggcgctg ctccacgttc tgagcttctc
     1321 ttccgtcctc acataattga catgcaattg cggcaatagt catactcttt tgcgatcatt
     1381 gtcggtgagg tcggcaagga ttatggagaa tcaacctcag agcagcgcta tacgacgtgt
     1441 gatggtgggc accgaccgtt ccaaaacggc ggatcacgcc gtccagtggg ccgctgaatt
     1501 cgccgaccgc tacggggcag agttgtttgt ggtgcaggtc atcgtgccgc aacacccgac
     1561 caccacagaa tacggcgccg ccgagcagac cagggccgct gccgccaatg atgagcttgc
     1621 gcatgttgtc gggcggctgg ccggagagcg agggcatgcc tttgtcgcca tagattccga
     1681 tccggcatcg gcaatcgtcc gtgccgccga gcgcgaagcc atcgatgtcc taatagtcgg
     1741 caattcgggc atggcggggc gaaaggagtt cttgcttggt aatgtcccga accgcatcag
     1801 tcacaattct cattgcaccg tcattattgt gaatacccta ccgtctgcgg gtgaaccgat
     1861 tcctgaatca gggcgaatgt ttcaacccca gggtgaacct cacactcccg agccacacct
     1921 ggggggacga gccgtgtata tcgcgacggt gacggcgaag catggtctca aggaactctt
     1981 cagccaaccg gatcaatcgg acgcttccac gcgacgtcag caggccaagc gcctacgcgc
     2041 ggcgttggaa gagctggggc cgaccttctc gaagcttgga caagtattgt cgacgcggcc
     2101 ggaccttctc cctccggaat acatcgaaga actgaccctg ttgcagagtc atgtccctcc
     2161 tatgcccgag agtgaggttg ttcgggtctc cgagcaagag ttgagggtgc cgtgggaaga
     2221 tgtgtttgaa tcaattgacc ccaagccgct tgccgctggg accatcg
//
LOCUS       DATNDD010000308         3239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3177181, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000308 DATNDD010000000
VERSION     DATNDD010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3177181"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VN647_07380"
     CDS             complement(<1..505)
                     /locus_tag="VN647_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWG96889.1"
                     /translation="MSEKIETLLKESRTYQPTAKTIAAAYIKDYETEYKQSIADPETF
                     WSTAAKELEWFSPWEKVLEWNYPWAKWFVGARCNITYNCLDRHIKTGRKNKVALVWIG
                     ENDQERIFTYAELHRQVNRCANALKKLGLQRGDRVTIYLPKIPEQVVAMLACARIGVI
                     HSVVYSGF"
     gene            676..1596
                     /locus_tag="VN647_07385"
     CDS             676..1596
                     /locus_tag="VN647_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWG96890.1"
                     /translation="MNSVRHIGKNLLFVSLCLLLSGAQVIGMFSVIALPTAEAQPGKP
                     EDLYYKSWAIIIGIENYVLAPPIPGAISDAKKVAEAFRRLGFDEVIEFYDKDASSRRL
                     HQLLNDMLPRKVGRMDRLVIFYVGHAGFMQDSDGQERGYLVPFDAQINNAAKSITVEH
                     LKEFTRRTASKHTLLILDAPVFGWETTAPAELSLEGRMAPESDTDRRAVQVISAARKG
                     EAAVRVEKSSRFVEALLAGLSGSADLDGNGWLMASELGGYLVGQVEAASNGAQRPWSL
                     RIDGDGDTALIEGRRSAFTLGADPQTPSEQ"
     gene            complement(1682..1852)
                     /locus_tag="VN647_07390"
     CDS             complement(1682..1852)
                     /locus_tag="VN647_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96891.1"
                     /translation="MADIQIEDGIIRILNLEIQDPKAAAVLAAYPQARWAEITRRAVK
                     IGLGYLKGGESD"
     gene            1997..2563
                     /locus_tag="VN647_07395"
     CDS             1997..2563
                     /locus_tag="VN647_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96892.1"
                     /translation="MIRSRAIHSRLVGPGLAVLLILGLAACGGPPKWVAKGSGVYNEK
                     DSKAFYGVGAVAGVRNAPLAWDTAENRGRAEIAKTFETYTGYLMRDYAASTTAGDFTR
                     NTEEQNVERAIKTITTTTLSGVRKIDQYMDPKTNTYYVLTKLSLEDMKNNLEQAKELN
                     SQVRDFVRRNADRLFERLEQEEEKRGVR"
     gene            2569..3219
                     /locus_tag="VN647_07400"
     CDS             2569..3219
                     /locus_tag="VN647_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HWG96893.1"
                     /translation="MVNELSTSLRRFAVIQRHRPSLVAMMSVALALSGCGHETKVTRV
                     DSGVVTDLSGRWNDTDSRMVAEAMVKDALQYPWLGNFEKTNQRQPVVVVGTVLNSSHE
                     HISVQTFITDLERELTNSQKVTFVAGKGERDEVRTERKEQAIYAREETQKAPGKEIGA
                     DFMMKGTIATILDEADGTKAVFYQVDLQMIDLENNAKVWYGQRKIKKVVERKRTIF"
BASE COUNT          753 a          802 c          941 g          743 t
ORIGIN      
        1 tgaaaccgga atagacgacg ctgtgaatca cgccgattct ggcgcaagcc agcatagcga
       61 caacttgctc aggaattttg ggcagataga tggtgacacg atcccctcgc tggaggccaa
      121 gctttttaag ggcgttggcg cagcggttca cttgtcggtg gagttcagca taggtgaaga
      181 ttcgttcctg atcgttttcg ccgatccaga ccagcgcgac tttgttcttg cgcccggtct
      241 tgatgtgccg gtccaggcag ttgtaggtga tgttgcatcg tgcgccgaca aaccatttcg
      301 cccatggata attccactcc aggaccttct cccaaggaga gaaccactcc aactctttgg
      361 cggccgtgct ccaaaatgtt tcagggtctg cgatggattg tttgtactcg gtctcgtagt
      421 ctttgatata ggcagcggct atcgtcttcg cggtcggctg gtatgtccga ctttctttca
      481 acagggtctc aatcttttcg ctcatggcag ccgcctctcc ttcttcagcg cagtcccttg
      541 tggatgcgta attatggaga aagtcgcgga tgcctgcaac catacagttg gtccgaggtc
      601 aactcgtcgt ggcgctgcgg gttgctctcg actccgttcc ttgacacctg tttaggccgg
      661 agataggctg acccgatgaa cagcgtaaga catatcggga aaaacctgtt gtttgtgtcc
      721 ttatgcctgc ttctatcggg tgctcaagtg atcggcatgt tctcggttat cgctctgccg
      781 accgctgagg ctcaaccggg aaagccggaa gacctgtact acaagtcctg ggcgatcatc
      841 attggaatcg aaaactatgt cttggctcct cccattccag gagcgatcag cgatgccaag
      901 aaggttgccg aggcatttcg acggttgggg tttgatgagg tgatcgagtt ctacgacaag
      961 gatgcaagct cgcgacgcct ccatcagctt ttgaacgaca tgttgccgag aaaagtggga
     1021 cgcatggacc ggctcgtcat cttctatgtg ggtcatgccg ggtttatgca ggactctgat
     1081 gggcaagagc gagggtatct tgtgcccttt gacgcacaga tcaacaatgc agcgaagtcg
     1141 atcacggttg agcacttaaa agaatttacg aggcggacgg cttccaaaca cacgctcctg
     1201 attctggatg cgccggtatt cggatgggaa accactgcgc cggcggagct gtcattggaa
     1261 ggacggatgg caccggaatc ggatacggat cggcgtgcgg ttcaggtcat tagtgcggca
     1321 aggaaaggag aggccgctgt tcgcgtcgaa aagagcagtc gctttgttga agccttattg
     1381 gccggacttt caggttccgc cgatctggac ggaaacggtt ggctcatggc ttccgagttg
     1441 gggggctacc tcgtgggaca agtcgaggcg gcttccaatg gtgcacaacg cccatggagt
     1501 ctacgaatcg acggtgatgg agacacggca ttgatcgaag ggcggaggtc ggcgtttacg
     1561 ctaggcgccg atcctcagac tccgtctgaa cagtaatagg aggctaagac tcaataagaa
     1621 cgtgccggtg cgctgcgtgc gtccgaacaa ccatcagtca agccgatcgc gctccgatgc
     1681 cttaatcact ctcgccgcct ttcaggtatc caaggccgat cttgaccgct cgacgggtga
     1741 tttcagccca acgtgcttga gggtaggccg cgagtaccgc cgcagctttg ggatcctgga
     1801 tctcaaggtt cagaattcta atgatgccgt cctcaatctg gatatctgcc acaaatctcc
     1861 cttccgagat gcgacggccg ctcgaaacgc atgtcgcgtt gactgatctc ggaacccatg
     1921 ttagcataat ttcaccgttt tttccaggga cattttacgt acggtaaata tacttattaa
     1981 cctcaaggag gattgtatga tccggtcacg cgcgattcat tccaggctgg tcggtcctgg
     2041 tcttgccgtc cttctcatct tgggactagc tgcttgcggc ggtcctccta aatgggttgc
     2101 aaaagggtcc ggagtctata atgaaaaaga cagcaaagcg ttttatggtg tgggggcggt
     2161 cgcgggggtt cgcaatgcgc cgcttgcttg ggatacggcc gagaatcgcg ggagggccga
     2221 gatcgccaag acattcgaga cctataccgg gtacctgatg agagactatg cggcctcgac
     2281 gaccgccgga gattttacac gaaatacgga agagcagaat gtcgagcgag ccatcaagac
     2341 cattacgacg acgacgctca gcggtgtccg gaaaatcgat cagtacatgg atcccaagac
     2401 caacacctat tatgtgttga ccaagttgag tttggaggat atgaaaaaca acttggaaca
     2461 agccaaagag cttaattcgc aagtacggga cttcgtacgg aggaatgcgg accgtctgtt
     2521 cgaacggctg gaacaggaag aggaaaagcg aggtgttcgc taatccacat ggtgaatgaa
     2581 ctgtccactt ctttgcggag gtttgctgtg atccagaggc atcgcccttc gcttgtcgcc
     2641 atgatgagtg ttgccctcgc tttgtcagga tgcgggcacg aaacaaaggt gacccgtgtc
     2701 gatagcgggg tcgttactga tttgagcggc cggtggaacg acacggattc tcgcatggtt
     2761 gccgaagcga tggtcaagga tgcgctgcaa tatccctggc tgggaaattt tgaaaagacg
     2821 aaccagcgtc aacccgtcgt cgtcgtgggg acggtcctga acagtagtca tgaacatatc
     2881 agtgtccaaa ctttcatcac ggatttggaa cgagaactca ccaattccca gaaagtcacg
     2941 ttcgtggccg gtaaaggcga gcgcgacgag gttcgaaccg aacggaaaga gcaggccatc
     3001 tacgcacgcg aagagacgca gaaagcccca ggaaaagaga tcggggccga tttcatgatg
     3061 aagggtacga tcgccaccat tcttgacgaa gccgatggaa ccaaagcggt cttctaccaa
     3121 gtggatcttc agatgatcga tctggagaac aatgcgaaag tttggtacgg acagaggaag
     3181 atcaagaagg tggttgaaag gaaacgcacc atcttttagg ttccgcggac gctgttgaa
//
LOCUS       DATNDD010000309         8049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_671327, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000309 DATNDD010000000
VERSION     DATNDD010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671327"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..952)
                     /locus_tag="VN647_07405"
     CDS             complement(38..952)
                     /locus_tag="VN647_07405"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWG96894.1"
                     /translation="MLEPVEKIWMDGKFVGWGEANVHVLTHSLHYGLAAFEGLRCYKG
                     KSGSAIFRLQEHVDRLFDSTHIGMMAMPYDKKQIADAIIETVRVNRLDACYIRPLVYI
                     GYGAMGVHPGDNPIRVAIAAWRWGSYLGDDALANGMRACVSSFTRHHVNVSMTRGKIS
                     GYYVNSIMAKRQAKADGYDEAILLDPEGYVAEGTGENVFIMRRGVLKTTPLTSVLEGI
                     TRNSIIQLAQEQKIIVVEERFTRDEMYIADEVFVTGTAAELTPVREIDNRLIGNGTPG
                     PITRALQDVFFSIVRGEDRAHESWLTRI"
     gene            complement(1083..1508)
                     /locus_tag="VN647_07410"
     CDS             complement(1083..1508)
                     /locus_tag="VN647_07410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG96895.1"
                     /translation="MPTSMLGAIIAMAPVSISRYKLRDGNEHMSHSNKFVIRTYHRIP
                     VRCEVYYLGGDFLGKGTVMNLCRNGFRVLGDHQVAPGMQLVVRLTLPDKDEPVEIQRV
                     VVRWVRGLLFGVKVVTMSPEGEDRVGTFLSSRLRAYCAS"
     gene            1639..1953
                     /gene="rplU"
                     /locus_tag="VN647_07415"
     CDS             1639..1953
                     /gene="rplU"
                     /locus_tag="VN647_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWG96896.1"
                     /translation="MYAIVETGGKQYRVETGTTIQVERLPGDIGAQIELSKVHLVHGN
                     DGILVGQPIINGAKVTAEIVRQGRTRSITVFKKKRRKNYRRTRGHRQGFTQLLIRNIA
                     TA"
     gene            1975..2238
                     /gene="rpmA"
                     /locus_tag="VN647_07420"
     CDS             1975..2238
                     /gene="rpmA"
                     /locus_tag="VN647_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWG96897.1"
                     /translation="MATNKGGGSSRNGRDSNPQYLGVKAYGGQTVTAGSIIVRQRGTK
                     FFPGFNVDLGRDHTLFAKMSGVVKFEGGRGRRKVSVYPVPVKS"
     gene            2364..3389
                     /gene="obgE"
                     /locus_tag="VN647_07425"
     CDS             2364..3389
                     /gene="obgE"
                     /locus_tag="VN647_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWG96898.1"
                     /translation="MFVDEIHITVRAGRGGNGICSFRREMFVPRGGPDGGDGGDGGDI
                     VMTASHRLTTLLDLRYQNHYAAQDGRAGGGSNCTGRSGEDLTIAVPVGTIVYDDLTKE
                     TLADFIADGQTVIIAQGGRGGKGNSNFATSVNRVPTKCTPGTPGEERTLRLELKLLAD
                     IGLVGFPNAGKSTLIAAISAARPKIADYPFTTLIPNLGVVRWGSDRSFVVADIPGLIE
                     GAHEGKGLGIQFLRHIERTAFLLHLIDVSEWAPDDPVIALETLRRELAAYDLDLAKRP
                     FVVVPTKIDVIGTSERLTQLQTYCRLNDSPCLPISAATNKGLDDLIAYLGKQVERLRK
                     TPCETNS"
     gene            3371..4492
                     /gene="proB"
                     /locus_tag="VN647_07430"
     CDS             3371..4492
                     /gene="proB"
                     /locus_tag="VN647_07430"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWG96899.1"
                     /translation="MRDKLLMQAKRIVIKIGSSLIASRAEGLRPQQIERLADEIAMLR
                     AQGLEILVVSSGAIVSGIRKLQLKDYPKSLPVKQAAAAVGQSRLMWAYEKAFERLNIQ
                     VAQILLTHQDLADRRRFLNARHTLTVLIGFGIVPIINENDTVAVDEIRVGDNDTLASE
                     VAHLADADLLVILSDVDGLYTEDPRKNPSATLIPLITEITQDIERLAGVSSTFEGTGG
                     MATKLRAAKKVGEYGISTLILNGERTGLLPTVLGGGPGGSLFLARERRLNSRKHWIAF
                     TLRSRGQVHLDQGAVDALTNRGKSLLASGVLRVMGSFEAGDPVGCVDTDGKEFARGLV
                     NVSSDLLRQMKGLKTADIHEQFGLQEYEEVIHRDNLAIL"
     gene            4516..5265
                     /gene="nadD"
                     /locus_tag="VN647_07435"
     CDS             4516..5265
                     /gene="nadD"
                     /locus_tag="VN647_07435"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWG96900.1"
                     /translation="MNSSNLDLQHSAFNTQHSPPQRLGLFGGSFNPIHNCHLTIAHHV
                     HERMQLSQVLFIPTGNPPHKRNGSLAPANVRFEMVRLAIADSPLFIASDIEIQRKGKS
                     YSIDTVRALQHHYGPSTELFFIIGLDAFLDFPTWKEPHELLKICHFVVVPRPERSFQA
                     LSEMPLLPNLDPPALASLDTGAQKGLDIVVPSCRGITCLALPPCPTSASEIRRRVRSG
                     LPLANILPPPVESYILCHSLYQEESDRTTPI"
     gene            5246..5668
                     /gene="rsfS"
                     /locus_tag="VN647_07440"
     CDS             5246..5668
                     /gene="rsfS"
                     /locus_tag="VN647_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWG96901.1"
                     /translation="MAPRPSKATALAVARAMLDKKALDVQVLHVAPLTSIADYLVIGS
                     AESDRQTRAIADSVAEVLSRVGQRPLSLEGTTSGQWVLIDFGDVVAHVFRHDTRSHYA
                     LERLWHDARSIPIPDNASPSVATLKGHVIQKATSRKMV"
     gene            5672..7069
                     /locus_tag="VN647_07445"
     CDS             5672..7069
                     /locus_tag="VN647_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96902.1"
                     /translation="MFKLLITIFIISAGVFIYSYFRELNPGTVVVHTAPGAEFELSPV
                     TLVLISMAFGAVLATLVVGLQQTAYLILNWRSNRLVRRKEKVDTLHRDGTHAFMSKRT
                     IEAITLLEKALAIDPNRVDSLLWLGNIYRSEQNYPEAIRLHQHAQRVDDRNIEILLEL
                     GKDLEEAKRYEEALQTLQNILKIEPDNLTALIRKRNLNIRMERWSEALEIQHRLLKAN
                     LPTQEKQAEAALLVGCMYEVGRQLLERGHPDKARRYFRGAIKKDRSFLPAYIGMGGIL
                     LHEGKTKDAVEILKKVYSRTRSVIILHRLEELFLDQGEPSEIIRVYQEALQQDPHNPI
                     LQFYLGKLYYRLEMVDEAYDLLSTIEGPQDHLLDYHKIMANLYLRKQHFEEAIVELKK
                     ALSFKKRVVVPYICTQCQQESIEWTGRCRRCAKWNTLTALPWLEAGHTTSPAGEPSSV
                     RAIPYQGIASPFETV"
     gene            7181..>8049
                     /gene="bioB"
                     /locus_tag="VN647_07450"
     CDS             7181..>8049
                     /gene="bioB"
                     /locus_tag="VN647_07450"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWG96903.1"
                     /translation="MTYMTLAEKALNDEPLTRVECRSVLTTPDDELLALLHAAFQVRS
                     KYFGRTVRLQMLQNAKSGACQEDCHYCSQSSISTAPIERYNLLPQKQMIEGARQAAAS
                     KAQRYCIVISGRSPLDREIDEIAGAVRSIKQEIPIQICCSLGLMNESQAKRLKAAGVD
                     RVNHNLNTSEAFHSSICTTHTFQDRLATIQNARTAGLEICSGGIVGMGESDEDVVELA
                     MALRDVKPDSIPLNTLHPVAGTPLEHCDSLTPQRCLKVLCLFRFVHPRTEIRVAGGRE
                     HNLRSLQPLALYPA"
BASE COUNT         1888 a         2288 c         2084 g         1789 t
ORIGIN      
        1 ggtggatgcg gttagaagcg ctcaggaata atgtgggcta gatacgagtc agccaggact
       61 cgtgcgcgcg atcttctcca cgcacgatcg aaaagaaaac gtcttgaagg gcgcgtgtaa
      121 tcggtccagg cgtaccattt ccgatgagtc ggttgtcgat ttctcggacc ggggtcaatt
      181 cagcagccgt tcccgtcaca aatacttcgt cggcaatata catttcatcg cgcgtgaatc
      241 gttcctccac cacaatgatt ttttgttctt gagccaattg aatgatggag tttcgcgtaa
      301 tcccttcgag caccgacgtc aacggcgtcg tcttgagaac acctcgacgc atgatgaaca
      361 cattctcccc tgttccctcg gcaacatagc cttcgggatc gagaagaatc gcctcgtcat
      421 atccatcggc cttcgcctgc cgtttggcca tgatggagtt cacatagtac ccggatattt
      481 ttcctctggt catggacaca ttcacgtggt gcctcgtaaa ggacgagacg caggcacgca
      541 tcccgttggc caacgcatcg tcccccaaat aggaacccca tctccatgcg gcgatggcca
      601 cccgaatcgg attgtctccc ggatggacac ccatcgcacc atacccgatg tagaccaacg
      661 gacgaatgta acaggcatcg agacgattga cacgaacggt ctcgatgatg gcgtcggcga
      721 tctgcttttt gtcatagggc atggccatca tgccgatgtg ggtcgaatca aacaatcgat
      781 cgacatgctc ctgaaggcgg aagatggcgg atcccgattt gcccttgtag caccgaaggc
      841 cttcaaacgc tgccaggcca tagtgtaacg aatgggtaag aacatggacg ttggcctccc
      901 cccaccccac aaatttcccg tccatccaga ttttttcaac gggttccaac attgaagacg
      961 atctccggtc aagaatcgta acagttccgt ctctatggtc gtgagggact atagcgtgag
     1021 gttgaacgtg ggtcaagcaa gccggccgga gacttctact cgactgatgc ggcgggcatc
     1081 tactaagagg cgcaataggc gcgcaggcgg gaactcagaa atgttccgac tcgatcttct
     1141 ccctccggac tcatggtcac gaccttgacg ccaaacaata ggccgcgcac ccatcgcaca
     1201 acgactcgct ggatttcgac cggttcatct ttgtcgggaa gggtgagccg aacgaccagc
     1261 tgcatcccgg gagccacttg atgatcgccc aggacacgga atccgttacg acacaggttc
     1321 atgaccgttc cttttccgag aaaatccccg cccagatagt acacctcgca gcgaacggga
     1381 atccgatgat aggtccggat cacaaactta ttagagtgag acatgtgctc gttgccgtct
     1441 ctcagtttgt atcgcgaaat actgacaggt gccatcgcga tgatggcgcc aagcatactt
     1501 gtcggcacga catccctcat agccttctaa agaggggcct attgagtgat caatgatctc
     1561 cggtcgctcc tcgtttagct tgacaccccc catatcccca tgttaaatgg acggttcctt
     1621 attctcggag aatagattat gtacgcgatc gttgaaacag gcggcaaaca atatcgagtt
     1681 gaaacaggga cgacgattca ggtcgaacgg ctgccgggag acatcggggc ccagattgaa
     1741 ctcagcaagg tccatctcgt acatggcaat gacggtattc tggttgggca gcccattatc
     1801 aacggagcca aggtcacagc cgaaattgtg cggcaaggac ggacccgatc gattaccgtt
     1861 tttaagaaga agcgccgcaa gaactatcgc cgcacccgtg gtcaccgaca aggattcacc
     1921 cagttgttga ttaggaatat tgcgacggcc taaagacaca ggaaggactt acccatggca
     1981 acaaacaaag gcggcggatc atcacgtaac ggccgtgata gcaatcctca atatttgggc
     2041 gtgaaggcct atggtggtca gactgtgacg gccggcagca ttattgttcg ccagcgtggc
     2101 accaagtttt tccctggatt caatgtggac cttggaagag accatacctt atttgccaaa
     2161 atgagcggtg tggttaagtt tgagggtgga cgcggcagac gaaaagtcag cgtatatccc
     2221 gttccggtca aatcctgatt ctcttttctg cctcctctgt tacgatcaca caggataaat
     2281 tctcctctca cctcgggcac ggtcgggtat actttctcat cagcaggtac cggagttacc
     2341 catctgtgga acgttgacag attatgtttg tcgatgaaat acatatcacc gtacgagccg
     2401 gtcgtggtgg aaacggcatc tgcagtttcc ggagggagat gtttgttccg cgtggaggcc
     2461 cggacggtgg ggatggaggc gacggcggcg acatcgtcat gaccgcatct catcgattga
     2521 cgaccctact cgatctccgc taccaaaatc attatgcagc tcaggacggg cgagccggtg
     2581 gaggatccaa ttgcacgggc cgttccggcg aagatctgac catcgctgtt cctgtcggca
     2641 ccatcgtcta tgatgacctg acgaaagaaa ctcttgcgga tttcatcgca gatggccaaa
     2701 cggtcatcat cgctcaaggg gggcgaggcg ggaaaggcaa cagcaacttt gccacttccg
     2761 tcaaccgagt gccgacgaaa tgcactcccg gaactccggg tgaagaacgg accttgcggc
     2821 ttgaactgaa actgctcgcc gacattgggc tggttggctt tccaaacgcc ggcaagtcga
     2881 cgctcattgc agccatatca gcggcccgac ccaagatcgc cgactatccc tttacaactc
     2941 tgatccccaa tctcggggtt gtccggtggg gatccgaccg aagttttgtc gtggctgata
     3001 ttcccggcct gatcgagggc gctcacgaag ggaaaggcct gggcatacag tttctccgcc
     3061 atatcgaacg caccgcgttc ttgctccact tgatcgatgt gtcagaatgg gctcctgacg
     3121 acccggtgat cgctcttgag accttacggc gggaacttgc cgcctatgat ctggacctcg
     3181 ccaagcgccc gttcgttgtt gtcccaacca agatcgatgt catcggcacg agcgaacgac
     3241 ttacccagct gcagacctat tgtcgtctca acgactcccc ctgcttaccg atatccgccg
     3301 ccacgaacaa agggcttgat gacttgatcg cctacctcgg gaaacaggtt gagcgtttac
     3361 gaaagacgcc atgcgagaca aactcttaat gcaagcgaaa cggatcgtca tcaagatcgg
     3421 cagcagcctg atcgcttcgc gcgcggaagg cctgcgcccg caacaaatcg agcgattggc
     3481 cgatgaaatc gcgatgctcc gagcacaggg cctcgaaatc ctggtggtct cttccggtgc
     3541 catcgtctcc ggcatccgaa agttgcagct gaaggattac cccaaaagcc tccctgtcaa
     3601 gcaggcagcg gcagcggtgg ggcaaagccg gctcatgtgg gcctatgaaa aagcatttga
     3661 acgtctcaat attcaagtgg cacagattct actgacccac caggatctcg ccgatcgccg
     3721 tcgatttctt aatgctcgtc atacgcttac ggtcctcatc ggtttcggca tcgtgcccat
     3781 catcaatgaa aacgataccg ttgcggttga tgaaatcagg gtcggcgaca atgacaccct
     3841 cgcgagcgaa gtggctcacc tggccgatgc ggacttgctg gtgatcctct ctgacgtcga
     3901 tggactgtat acggaagatc cgcgtaaaaa cccatcggcg acattgatcc cgctgatcac
     3961 ggagattacc caagacatcg aacgcctggc aggagtatcc agcacattcg aagggacagg
     4021 cggaatggcg accaagcttc gagcggccaa gaaagtcggc gagtatggaa tctcgacgtt
     4081 aatcctcaat ggcgagcgga ccggactcct cccaacggtt cttggaggcg ggcctggtgg
     4141 aagtctgttt ctcgcccggg agcgtcgact gaacagccgt aaacattgga tcgcctttac
     4201 gcttcgctcc cgaggccagg tccatctcga ccagggggct gtggacgctt tgaccaatcg
     4261 agggaaaagt ctgctggcat caggcgtcct tcgagtgatg ggatcgtttg aagccggcga
     4321 tccggtcggt tgtgtcgata cagacggaaa ggaattcgca agaggcctgg tgaacgtttc
     4381 gtccgaccta ttacgtcaaa tgaaaggcct caagacagcg gacattcatg aacagttcgg
     4441 actccaagag tatgaagaag tcatccaccg agataattta gccatcctct agtgctgagc
     4501 cttgcgtgct gagtcatgaa ttcttctaac ctagaccttc agcactcagc attcaacact
     4561 caacattccc ctccgcaacg cctcggctta ttcggtggaa gcttcaatcc tattcataat
     4621 tgtcacctga ccattgccca tcacgtacat gaacgtatgc agctctccca ggttctattc
     4681 atcccgaccg gcaacccgcc ccataaacgg aacggctcac tggctccggc gaatgttcgc
     4741 ttcgaaatgg tacgtcttgc gattgccgac agcccacttt tcatagcctc ggatatcgag
     4801 atacagcgaa agggaaagtc ctactcgatc gatactgtcc gcgcattaca acaccactat
     4861 gggccatcca ccgagctgtt ctttattatt gggctcgacg cgtttcttga cttccctaca
     4921 tggaaagaac cccacgagct tttaaagatc tgtcatttcg tagtggtgcc tcgacctgag
     4981 cgatcgttcc aggcgctgtc cgaaatgccg ttgctcccca acctggatcc accagctctt
     5041 gcctcgctcg acaccggcgc tcagaaggga ctcgacatcg tagtcccttc ctgccggggg
     5101 atcacctgcc tggcccttcc accctgtccg acctctgcct cagaaattag acggagagtt
     5161 cggagcggac taccgctggc aaatatattg cccccccccg tagaatccta tatactttgt
     5221 catagccttt atcaggagga gagcgatcgc accacgccca tctaaggcca cagccctagc
     5281 cgtagccagg gcgatgctcg acaagaaggc ccttgatgtc caagtcctcc acgttgcccc
     5341 gcttacttca atcgctgatt acttggtcat tgggtcagct gaatccgacc gacaaacacg
     5401 cgcaatcgct gattccgtcg ccgaggtact ctcgcgcgtg ggccaacgac cgctgagtct
     5461 cgaaggcacc acatccggcc agtgggtcct gatcgatttc ggcgatgttg tcgcccatgt
     5521 gttcagacac gacacgcgct cgcattatgc tcttgagcgc ctgtggcacg acgcccgatc
     5581 tatcccgatt ccagacaatg cgtcgccttc ggtcgccaca ctgaagggac acgtgatcca
     5641 gaaggcgacc tcacggaaga tggtctaggc catgttcaaa ctgctgatca ccatttttat
     5701 tatcagcgcc ggcgtgttca tttacagtta tttccgggag ttgaacccgg gaacagtcgt
     5761 cgtccatacc gcgccgggcg ccgagtttga gctcagtccc gtcacacttg tgttgatttc
     5821 tatggccttt ggagcggtac tcgcgaccct tgtcgtaggg ttgcaacaaa cggcatacct
     5881 gatcttaaac tggcgcagta accgcctcgt ccgtcgcaag gagaaggtcg atacgctcca
     5941 ccgagacgga acgcatgcat ttatgtcgaa acgcaccatt gaggcgatca cactcttgga
     6001 gaaggccctg gccatcgatc ctaatcgagt cgattcgctc ctgtggctgg gaaatatcta
     6061 ccgatcagag cagaattatc ccgaagcgat ccgactccat cagcatgcac aacgggtgga
     6121 cgatcgaaac atcgaaatct tgttggaatt aggcaaggat ttggaagaag ccaaacgtta
     6181 cgaggaagcg cttcaaacac ttcagaatat tcttaagatt gaaccggata atctgacggc
     6241 gctcattcgg aagcggaatc tcaatatccg tatggagcgg tggagtgaag cccttgagat
     6301 tcaacaccgt ttgctcaaag ccaatcttcc cacccaggaa aagcaggcgg aagcggctct
     6361 gctggtcgga tgcatgtatg aagtcgggcg tcaattgctc gaacgcgggc atcccgataa
     6421 agcccgtcga tacttccgag gcgctatcaa gaaggaccga agctttctcc ccgcctatat
     6481 cgggatgggc ggaattctgc tccacgaagg caaaaccaaa gatgccgttg aaatcctcaa
     6541 gaaggtctat tcacggacgc gaagcgtgat cattctgcat cgattggaag aacttttctt
     6601 ggatcaaggc gagcctagtg aaatcattcg agtctatcag gaggccttac agcaagatcc
     6661 gcacaatccg atcctccagt tctacctagg taagctctac tatcggctgg aaatggttga
     6721 tgaggcgtac gacctgctgt cgacgatcga aggcccgcag gaccatctgc tggactacca
     6781 caagatcatg gccaacctgt acttgcggaa acagcatttt gaagaggcta tcgttgaatt
     6841 gaagaaggcc ctcagtttca agaagcgcgt agtcgtccct tatatctgca cacagtgtca
     6901 acaggaatcc attgagtgga cgggccgctg ccgccgctgc gccaagtgga acacgctcac
     6961 cgcccttccc tggcttgaag ccggtcacac taccagcccg gctggagagc ccagctccgt
     7021 ccgtgctatt ccctatcaag gcattgcttc tccgtttgaa accgtgtagg ttctcgccag
     7081 cctctgttga ttgttcggtc taccggtttg tggagacctg ctcctcgatt cgacaaattt
     7141 gaagtacagc agattaccac gtcattactt tgaggattcc atgacctata tgactttggc
     7201 agaaaaagcg ctcaacgacg aaccccttac cagagtagag tgccgttccg tcttgacgac
     7261 gcccgatgac gaattgctcg cgttgttgca tgcagccttt caagtccgct ccaaatactt
     7321 cggtcggacc gtccgcctcc agatgttgca gaatgccaag agcggagcct gccaagaaga
     7381 ctgtcactac tgttctcaat cttccatctc gacggcgccg attgagcgat acaacctgct
     7441 tccccagaag cagatgatcg aaggtgcgcg acaagctgcc gcctcaaaag cccagcgtta
     7501 ttgcattgtc atcagtgggc gaagcccttt ggatcgggaa atcgacgaaa tcgccggagc
     7561 cgttcgttcg atcaagcagg agattccgat ccagatctgt tgctccctcg gcctcatgaa
     7621 tgagtctcag gccaaacgac tgaaagccgc aggtgtcgat cgcgtgaatc acaacctgaa
     7681 taccagcgaa gcctttcact catcgatctg caccacccac accttccaag atcggctcgc
     7741 gacgattcag aatgcccgaa cagctggact agaaatctgt tcaggtggca ttgttggcat
     7801 gggcgagagc gatgaggatg tcgttgaact agcgatggct ctgcgcgacg tgaaaccgga
     7861 ttccattcca ttgaatacgc tgcatccggt cgccggtaca ccgttggaac attgtgattc
     7921 cctcacacct caacgctgtt tgaaagtgct ctgcctcttc cggttcgtgc accctcgaac
     7981 ggagattcgc gtcgcgggcg gccgcgaaca taatctgcgc agtttgcaac ccctcgccct
     8041 ctaccctgc
//
LOCUS       DATNDD010000310         4271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_171390, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000310 DATNDD010000000
VERSION     DATNDD010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171390"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VN647_07455"
     CDS             complement(<1..258)
                     /locus_tag="VN647_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96904.1"
                     /translation="MTQPEERTASTARRYEWLTFVMVLITLFTLGVGTFLLGHVERSI
                     VAAVWLPLLMLLLWSTIRLRAEYRQANQESAWARAAEAALLQ"
     gene            complement(255..1805)
                     /locus_tag="VN647_07460"
     CDS             complement(255..1805)
                     /locus_tag="VN647_07460"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HWG96905.1"
                     /translation="MIGSEPSLLQFGMHRMIGISMDLRTVDSKQTLRVLPQLVPDSAC
                     FRCDVCCRFPEADSFLRPYFTRSEISDAVEHGIPESFFPDKFGSQVGLVKNPIGEGYL
                     CPAFDSKSGRCGIYDVRPLDCRLYPMALMWDLSGREVLLGWDSKCPFMRDAPSSAIRE
                     YAERVAIFLAGEAIVETIAAHPRFIGSFQDDVVVLKTLPHLTARLGPQRVDFRLHPLT
                     VSDGSYFEKALERAQVLCPETPAAFAFPYHYIWSSLLPYWWMEFDRTLFLFVQSPDGW
                     FMPLPPLGAGPLDRAVEQAFALMRSWNGTSPVSRIENVMESQRQLLECDGIRFRRKEG
                     DYLYPVEALAALAGDHYKSQRALCNRAAREQAITKEPYRTDDHSGCARLYRRWAEQKH
                     RGNLDQVGKLLLEDAEMAHALVFQEHERIGLSGTVLRVNHDIVAYTFGYWLTPQTWCI
                     LLEVADRSIPGLAQWLFRETCRTAMTQGAVSINAMDDAGLPGLRAAKSAYRPSAILDT
                     WVITRMTT"
     gene            1937..3367
                     /locus_tag="VN647_07465"
     CDS             1937..3367
                     /locus_tag="VN647_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96906.1"
                     /translation="MAQPESSCPTLSPEAARDRCIAVRTRLQNAGLFGVTAAWRVSPC
                     PFYLSSDQLQFFINLGPQLLSFYRALNRLYNESVKGIQPAWISKYLDQGKPDALVQYS
                     RMKRFREALPTVIRPDVIPTEDGMVITELDSVPGGIGLTACLSYSYHDLAGGDSQLIG
                     GRDGMVRGFARMLRSVQGRREGCFAILVSEESKDYRPEMSWLAAQLREAGMPVWCVEP
                     QEIRFTEEGLRLHIDGSERAIGMVYRFYELFDLLNIPKAELVQYAAKKGRLSVTPPYK
                     PTLEEKSAFALLHHPKLRPFWEKELGADGMLHVSAIMPKTWLLDPAPLPAIATIPGLR
                     IGGRAVTNWSELETATQKERHFVIKPSGFSALAWGSRGVSIGHDMPQSAWVEALRNAL
                     ASFPATPYILQEFHKGRLFEMDYLDEGMQTIVRMSGRARLSPYYVVSDDKAELTGILA
                     TICPADKKILHGMKDAIMVPCALRSN"
     gene            3694..4026
                     /locus_tag="VN647_07470"
     CDS             3694..4026
                     /locus_tag="VN647_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWG96907.1"
                     /translation="MTLFHVDWCPDCHVVRQKLADLGLTYEAVVVPDSRRMRTQVYEV
                     SGQYYVPVLKDGDLVLTETDDILAYLDERYSADRAETNAATEFQSQARTTPEVLGKDD
                     EHPSCRIN"
     gene            4044..>4271
                     /locus_tag="VN647_07475"
     CDS             4044..>4271
                     /locus_tag="VN647_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HWG96908.1"
                     /translation="MEDWRQVLAQSIVKPKDLAERFGLDEKEIEAIVGPYPMRITPTV
                     LETIKSKDDAIWKQVVPDPAELHDIVADDDPL"
BASE COUNT          969 a         1246 c         1189 g          867 t
ORIGIN      
        1 ttgcagtaag gccgcctccg cagccctcgc ccaggcgctt tcttggttgg cttgtcgata
       61 ttccgctcgc aatcgaatcg tcgaccacaa cagcaacatc aacagcggaa gccagacggc
      121 ggccacgatg ctgcgttcga catggccgag cagaaaggtc cccacgccta aagtaaacag
      181 cgtgatcaag accatcacga acgtcaacca ctcatatcgg cgagccgttg aagcggttcg
      241 ctcttccggt tgggtcatgt ggtcatcctt gtgatcaccc aggtgtccag gattgcggat
      301 gggcgataag ccgacttggc agcacgtagt cccggaagac ctgcatcatc catggcattg
      361 atagagacgg ccccttgggt catggcggtt cgacaggtct cgcggaagag ccactgagcc
      421 agaccgggaa tggaacggtc tgcgacctcc aacaagatgc accaggtttg aggagtcaac
      481 cagtagccga aggtgtaggc gacaatgtca tgattgactc tcaggacagt acccgacaag
      541 ccgatccgtt catgctcttg aaagacgagc gcgtgggcca tttctgcatc ctccagaagc
      601 agcttcccga cctggtcgag atttccccgg tgtttctgct cggcccaccg ccgatacaga
      661 cgcgcgcagc cggagtgatc gtcagtccga tagggctctt tcgtgatcgc ctgttctcgg
      721 gccgcacgat tgcagagcgc ccgttgtgac ttatagtgat ctcctgccaa ggcagccaga
      781 gcctcgacag ggtagagata gtctccttct ttccgacgga atcgaatccc gtcgcactcc
      841 agcagttgtc gttgggactc catcacgttt tcgattcgac tcaccggcga cgtcccgttc
      901 catgaccgca tcagcgcgaa cgcttgctcc accgcccgat ccaaaggacc ggctccgagc
      961 ggcgggagcg gcatgaacca cccgtccgga gactggacaa aaagaaacaa cgtgcgatcg
     1021 aactccatcc accagtaggg aagcagcgat gaccatatgt aatggtaagg aaacgcaaac
     1081 gcggcgggtg tctcagggca gagtacctgg gcccgttcga gcgccttctc gaaatacgag
     1141 ccgtccgata ctgtgagtgg atggagccga aaatcaaccc gttgcggccc gagacgagcc
     1201 gtcaggtgag gcagcgtctt gagcacgacc acatcgtctt ggaaggaacc gatgaagcgg
     1261 ggatgagccg caatcgtctc cacgatcgct tcacccgcaa gaaagatcgc gacacgttct
     1321 gcatattcgc gaatcgcgct cgagggtgcg tcgcgcatga acggacactt ggaatcccaa
     1381 ccaagcagaa cttctcgacc cgacaggtcc cacatcagcg ccatcgggta cagccgacaa
     1441 tccaaggggc gaacgtcgta aattccgcac cggcccgact tcgaatcaaa agccggacag
     1501 agataccctt caccgatcgg gttcttgaca agaccaacct gcgagccgaa cttatcggga
     1561 aagaacgatt cggggattcc atgttccacg gcatcgctga tttccgaacg tgtaaagtat
     1621 gggcgaagaa aactatcggc ttccggaaaa cgacaacaca catcacaacg aaaacatgcc
     1681 gaatccggaa ccagttgagg cagaaccctc aatgtctgtt tggaatccac tgtcctgagg
     1741 tccattgaga tcccaatcat acgatgcatg ccgaactgta gcaaggaagg ttcgcttccg
     1801 atcatctcgc gatgtgtcca tcctcatatc catcgggcag ccggccgccc cttgtcggtc
     1861 tccgatgtgc atgctagcat gagcttcttt gccggacgct ctcacaatag cccgcagcat
     1921 gctaagggat tttccgttgg cacaacctga atcttcctgc ccgacgctct ctcctgaggc
     1981 ggcacgcgac cgctgcatag ccgttcggac acggttacag aacgccggtc tgttcggtgt
     2041 gactgcggct tggcgggtca gcccctgtcc gttttatctg tcttccgatc agctgcaatt
     2101 tttcatcaat ctggggccgc aactgctctc gttctaccga gcgctgaatc ggctctacaa
     2161 tgaaagcgtc aaaggaattc aaccggcctg gatatcaaaa tatctcgatc agggcaagcc
     2221 ggacgccttg gtccagtaca gccggatgaa acggtttcgc gaggccttgc ccaccgtcat
     2281 caggcccgat gtcatcccca cagaggacgg catggtcatc accgagctgg attcggttcc
     2341 aggcggcatc gggctcaccg cctgcctctc gtacagctat cacgaccttg ccggcggtga
     2401 ttcacaactc atcggggggc gagacggaat ggtacgcgga tttgcgcgca tgctgcgatc
     2461 tgtgcaaggc cggcgtgagg gatgctttgc gatcctggtc tcggaggaat caaaagacta
     2521 tcggccggaa atgtcctggt tggccgctca actccgcgaa gcggggatgc ccgtttggtg
     2581 tgtcgagccg caggagatcc gatttacgga ggaaggcctt cgattacata tcgacggcag
     2641 cgagcgagcg atcggtatgg tctaccgttt ctatgaacta ttcgacctgt tgaacattcc
     2701 caaggccgaa ctcgttcaat atgccgcaaa gaagggccgg ctatccgtga cgccgcccta
     2761 caagccgaca ctggaagaaa aatccgcgtt cgcgctgctg caccatccga agctccgtcc
     2821 cttctgggaa aaggaactcg gagcggacgg catgcttcat gtgagcgcaa tcatgccgaa
     2881 gacctggctg ctcgacccgg cgccgcttcc tgcgattgcg acgatccccg gcctacggat
     2941 cggagggcga gcggttacaa attggtccga actcgagacg gcgacgcaga aggagcgtca
     3001 tttcgtcatc aagccttccg gtttttccgc actcgcttgg ggaagtcgcg gtgtgtcgat
     3061 cgggcatgac atgcctcaga gtgcatgggt tgaggccctc cggaacgcgc ttgcatcctt
     3121 cccggccaca ccctatatct tacaagagtt ccataaggga cggttgttcg aaatggacta
     3181 tttggatgaa ggtatgcaga cgatcgtgcg catgtccggg cgagcgcggt tgtctcccta
     3241 ttatgttgtc tcagacgata aagcggaact caccggcatt ctggcgacga tctgcccggc
     3301 cgacaagaag atccttcacg gcatgaagga cgcgattatg gttccttgcg ccctacgatc
     3361 gaactaagct cctcaataaa tcctaacagt attgaaaaaa caatgaatgg tcagcaggat
     3421 gttcaaaaag gccgtcgttc tcacccgccc accccggcgc gccaaaacgc gccgttccac
     3481 gggcaaggcc tcaggctagg tgcatgctgt tcggtacgtt gagcttctga ggttcacgcc
     3541 gtgccggata aggcacggca cgtctgtgaa cgccgccgag atagtgaggc ggcagtgtcc
     3601 cacgagaacg atgctggagg cctttttcaa catcctgcca aaatgggcgt ttcactttct
     3661 tccatttcct ggtagcctaa taggattgtg gctgtgactc ttttccatgt cgactggtgc
     3721 cctgactgtc acgtggtccg ccaaaaactg gctgacttgg gattgaccta tgaggctgtc
     3781 gtcgtgcccg acagcagacg gatgcgcacg caggtatacg aggtctccgg ccaatactat
     3841 gtgccggtcc tcaaggacgg cgatctcgtt ctgacggaaa ccgacgacat tctggcctat
     3901 ctggacgagc ggtatagtgc cgatcgcgca gaaaccaacg cagcaaccga gtttcagtca
     3961 caagcccgta cgacgcctga ggttttgggc aaagacgacg agcacccttc ctgccggatt
     4021 aactgatcaa cgaaggacga ttcatggaag actggagaca agtcctggcc cagagcatcg
     4081 tgaagcccaa ggacttggcc gaacgattcg gtctcgatga gaaagagatt gaagccatcg
     4141 tcggccctta tcccatgcgg atcaccccga cggtactcga gacaatcaaa tcgaaggatg
     4201 acgcgatctg gaagcaggtt gtccccgatc ccgcagagct gcacgacatt gtggccgacg
     4261 atgatcccct t
//
LOCUS       DATNDD010000311         3329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1545117, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000311 DATNDD010000000
VERSION     DATNDD010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545117"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VN647_07480"
     CDS             <1..458
                     /locus_tag="VN647_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWG96909.1"
                     /translation="AAPLAALMIARLPPLNSVDVIIPVPLHIQKLREREFNQSLLLAD
                     HIGRRLDIPVASTNLVRTAPTPAQTTLSRKSRLKNLRRAFAVRRPDAIVNKRILLIDD
                     VFTTGTTVNECAKALRRAGSADVFAITLSRTVDTDIVPDRILAQHPYFF"
     gene            477..818
                     /locus_tag="VN647_07485"
     CDS             477..818
                     /locus_tag="VN647_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWG96910.1"
                     /translation="MPIYEYSCQECRKRSSFLVMNPRRPRSIVCRHCGSLKLERLLSR
                     FAAPKSEESRLESLADPANLGGLDESDPRSVARLMKKMGEEMGEDVGDMEAMLDQPGD
                     GDGTIDHSDNF"
     gene            925..1122
                     /locus_tag="VN647_07490"
     CDS             925..1122
                     /locus_tag="VN647_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWG96911.1"
                     /translation="MSKGPKTELMTATETCRYLKITQRTLYRYLRNRQIPAFKLGNEW
                     RFVRSDLEQWTRDRTGATVNS"
     gene            1138..1578
                     /locus_tag="VN647_07495"
     CDS             1138..1578
                     /locus_tag="VN647_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96912.1"
                     /translation="MFAGEYLCKVDEKGRFIVPSPIREQIEADGQTVMFLKGPEQSLL
                     IYSTKEWEKVLERTRTSLDEDQSRLFMHFVVSEASSSDIDKTGRILIPGRLRKLVPVD
                     EDQEIILVGLYHRMEIWNPSEWRRYLARTEDRFEQNMAKILNLL"
     gene            1588..2184
                     /locus_tag="VN647_07500"
     CDS             1588..2184
                     /locus_tag="VN647_07500"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HWG96913.1"
                     /translation="MPSDRRQYNYRSSRFRLPTTWRRGDPICQSTPKRLPRINGTALP
                     SQSDPSNSKTALCTPSYPSFAISDTFKITLPVPPSINHQYATVNGRRLLSAAGRAYKT
                     YVGQQVWLALAQSSSRFSLRDRLQSGPLALTIRFFFASALRRDLDGGLKIAQDAICQG
                     LGLNDNRIIETHLYKQVDKADPRMEVSLSFLHPPHHSA"
     gene            2286..2939
                     /locus_tag="VN647_07505"
     CDS             2286..2939
                     /locus_tag="VN647_07505"
                     /inference="COORDINATES: protein motif:HMM:NF023299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HWG96914.1"
                     /translation="MATTRIPVRDLQIGMYVARLDLSWFRSPLLRHSFLIEYPSQIDK
                     LVRAGVKMVDIDLDRGIASQPHQVSGTTHSVTEAAPTWNIKKQSKPLAQLNEEYVQAK
                     LAKQQMDQAVQSVFATITKTGTVNPQQAVEAVQEITIAMRTLTDSAIFMALSQNRAGD
                     STLSRHALATCTLSLVLGQAFQFNPLELQELATAALLHDIGLLQLQGVELEGLSYNQ"
     gene            complement(2874..>3329)
                     /locus_tag="VN647_07510"
     CDS             complement(2874..>3329)
                     /locus_tag="VN647_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96915.1"
                     /translation="KLVRAGVKMVDIDLDRGIASQPHQVTDTMHAATEASPTWSMKKQ
                     FKPLAQLNEEYVQARLAKQQMDQAVQSVFTTITKTGTVNPQQAAEAVQEITIAMRTLT
                     DSAIFMAVSQNRAGDSTLSRHALATCTLSLVVGQTFQFNPLELQESDVM"
BASE COUNT          766 a          946 c          823 g          794 t
ORIGIN      
        1 tggcggctcc tcttgccgcc ctcatgatcg ccagactgcc tcctctcaat tccgttgatg
       61 tgatcatacc ggtgcccttg cacatccaga aactccgtga acgagaattc aatcagtctc
      121 tgcttcttgc cgatcacatc gggcgtcgcc tcgacattcc agtggcatct accaatttag
      181 tccgaaccgc ccctacgccg gcccaaacca cattgtcccg caaaagccgt ctaaaaaacc
      241 ttcgccgagc gtttgccgtc cgacgaccag atgcgatcgt caacaagcgc attcttctga
      301 tcgacgacgt cttcacaacc ggtaccacgg tgaatgagtg cgcaaaggcc ctgcgcaggg
      361 ctggatctgc cgacgtattt gccataacgt taagccgaac cgtggatacc gatattgtcc
      421 ccgaccgaat tctggctcaa catccgtatt ttttttgaaa tttggagggt caatctatgc
      481 cgatttacga gtactcgtgt caggaatgtc gcaagagaag ttcgttcctg gtcatgaacc
      541 cccgtcgtcc tcgttcgatt gtttgtcggc actgtggcag cctgaaattg gagcggctcc
      601 tgtcccgatt cgctgcaccc aagtcggaag agtccagact cgaatcactc gcggaccctg
      661 cgaacctggg tgggcttgat gagtctgatc ctcggagtgt cgcccgtctc atgaagaaaa
      721 tgggtgaaga gatgggggaa gacgtaggcg acatggaagc tatgttggac caacccggcg
      781 atggtgatgg gacaatagat catagcgaca acttctgaca tgtaacgtca tatattttgg
      841 cttgacattc ttatggtggt ctctagaata gcctgagtac cggaggcagt atgggtcttt
      901 ctcattctgg taacgggtgg agtgatgagc aaaggtccga agaccgaact gatgacagcg
      961 acagaaacat gccggtatct gaagattact caacgtaccc tttatcgcta tcttcggaat
     1021 cggcagattc ccgccttcaa gctgggaaac gaatggcgat ttgtacggtc ggacctcgag
     1081 caatggaccc gcgaccgaac cggagccaca gtgaactcct aaacacagga catcgcaatg
     1141 tttgccggtg aatatctttg caaggttgat gaaaaagggc gtttcatcgt cccctcacca
     1201 atccgcgaac agatcgaagc cgacggtcag acggtgatgt tcctaaaagg tccggaacag
     1261 tcgttgttga tctattccac caaagaatgg gagaaggttc ttgaacggac caggacgtct
     1321 ctggacgaag accagagccg cctgtttatg cactttgtcg tgtccgaggc cagctcatcg
     1381 gacatagata aaaccggccg gatcctgatt cccggtcgcc tgcggaaact ggttccggtc
     1441 gatgaagatc aagagatcat cctcgtcggc ctctatcatc gaatggaaat ttggaatcca
     1501 agcgaatggc gtcggtatct tgcccgcacg gaagatcgct ttgagcagaa tatggcaaag
     1561 atcctgaacc tcctttagtt cggctccatg ccgtccgacc gccgtcaata caattaccgt
     1621 tcatccagat ttcgccttcc cacaacatgg cgtcgtggtg atccaatctg ccaatccaca
     1681 ccgaaaaggc taccacggat caacggtacg gctctgccgt cacaatctga tccttcgaac
     1741 tccaagaccg ctctttgcac accatcgtat ccctcgttcg ccatatcgga tacattcaag
     1801 attacactgc ccgtccctcc cagcatcaac catcaatatg cgacagtcaa tggacgtcgt
     1861 ttgctatcgg ctgccggacg tgcctataaa acatacgtcg gccagcaagt atggctggca
     1921 ttggcccagt cttcttctag attctccctg cgggatcgtc ttcaatctgg accgctcgcg
     1981 ctcactattc gattcttttt tgcttccgcc ttacgacgcg acctcgatgg tggtctaaag
     2041 atcgcccaag atgcgatctg tcaaggactg ggcttaaacg acaatcgcat cattgaaacc
     2101 catctgtaca aacaagtcga caaagcggat cctcgcatgg aggtctctct ctcattcctt
     2161 cacccgcctc atcactcggc ctgaccactc accgcatcac ccgctcagct acagttatcc
     2221 cccgttcgtg ccgatacctg aggagctatt tctgcaaccc tggtcacgtg caatccggac
     2281 ctccgatggc gacaactcgc atccccgtcc gtgatcttca gatcggaatg tacgtcgccc
     2341 gcctggatct ttcgtggttt cgctctcctt tactccgcca ttcatttctg attgagtatc
     2401 cttcgcagat cgacaaatta gtacgcgcag gggtgaagat ggtcgacatc gacctcgacc
     2461 ggggaatcgc ttctcagccg catcaggtat cgggtaccac gcattctgta accgaggcgg
     2521 cacctacgtg gaatattaag aagcaatcca agcctttggc tcagttgaac gaagaatatg
     2581 tccaagcgaa actggccaag caacagatgg atcaagccgt acaatccgtt tttgccacga
     2641 tcaccaaaac agggacggtc aatcctcagc aggcggtcga agcggttcaa gaaatcacga
     2701 tcgctatgag aaccctcacc gactccgcta tcttcatggc attgagtcaa aatcgggccg
     2761 gcgattccac tctcagccgc catgcactgg ccacctgtac gctgtcactg gtcttaggcc
     2821 aggccttcca attcaacccc ttggaactgc aggagctggc gactgccgcg cttttacatg
     2881 acatcggact cttgcagctc caaggggttg aactggaagg tttgtcctac aaccagtgac
     2941 aatgtacagg tggccagtgc atggcggctg agagtggaat cgccggcccg gttttggctc
     3001 actgccatga aaatggcgga atcagtgaga gttctcatcg cgatggtgat ttcctggacc
     3061 gcttcggccg cctgctgagg gttgaccgtc cccgttttgg taatagtcgt aaaaacggat
     3121 tgtacggctt gatccatctg ttgcttggcc agcctcgctt ggacatattc ttcgttcagc
     3181 tgagccaaag gcttgaattg ttttttcata ctccaagtag gtgaggcctc ggtcgcagca
     3241 tgcatggtgt cggttacctg atgcggctga gaagcgattc cccggtcgag gtcgatgtcg
     3301 accatcttta cccctgcgcg caccaattt
//
LOCUS       DATNDD010000312        10270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2641891, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000312 DATNDD010000000
VERSION     DATNDD010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641891"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="mrtJ"
                     /locus_tag="VN647_07515"
     CDS             <1..571
                     /gene="mrtJ"
                     /locus_tag="VN647_07515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF033192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="JDVT-CTERM system glutamic-type intramembrane
                     protease"
                     /protein_id="HWG96916.1"
                     /translation="QSQRTLDLDRMVVAQGVSFRVLHLLSRSAQVMVLLGFQYNRPFH
                     RDSAFLLLLALGPIVWISMIEVFPFQPLPRHTIWSPAFLSIALWQPLFEELLFRGIVQ
                     GRLLQSTARRKTWIGLSVSNLLTSLLFSLAHLASHSVSWSLLVFVPSLCFGFVRDRFG
                     SVHPSITLHAFYNAGYFLLLGGLTLPNSF"
     gene            complement(674..1696)
                     /locus_tag="VN647_07520"
     CDS             complement(674..1696)
                     /locus_tag="VN647_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWG96917.1"
                     /translation="MVKLISLLSPLFAGFLGIAPTAPTQSAQLEQDRGTWHAAAPLPT
                     KRTEVAAAMLEGKIYVVGGFEKPGVGNLLNFAITPSVEMYDPVTDRWTPKTPMPVGLH
                     HVGIGVVDGRLYVIGGYSRSGLSVWSPVATVYAYDPVTDTWTGRAPMPTARGALAVTE
                     HEGKLYAIGGYDRKANNVQVEVYDPVRNVWTTAASLPTSRDHLGTATVAGKIYAIGGR
                     IDGDYHRNLAVMEQYDPATDRWRRVADLPTARSGITAAAVEGRIYVFGGEGSSGTFSE
                     NEVYDPASDTWQRMAPMPTARHGIGSAVVRGRIYVISGGPTPGGSFSDLNEVFTIPST
                     SISMAE"
     gene            complement(2036..2251)
                     /locus_tag="VN647_07525"
     CDS             complement(2036..2251)
                     /locus_tag="VN647_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96918.1"
                     /translation="MRLVSNPTETPTDDPRRWIGGEATVGSQARRYIRRQAGTTNSLR
                     GVILGLTFLGGLALGSSFCLLCRAKSK"
     gene            complement(2226..2663)
                     /locus_tag="VN647_07530"
     CDS             complement(2226..2663)
                     /locus_tag="VN647_07530"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG96919.1"
                     /translation="MTCKRTLEQSATWETGQVLFPGEKGILSSMERRRHRRVPAQVKS
                     LLRANSHEVEGEVLDLSLGGALVESSLEVQPGKQIAVKLIVPGDDIPILIEQARVQWT
                     VDRTFGVRFVDVQHQEMDELEQLIDECIALDEGRRHETREQSN"
     gene            2691..4307
                     /gene="lnt"
                     /locus_tag="VN647_07535"
     CDS             2691..4307
                     /gene="lnt"
                     /locus_tag="VN647_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWG96920.1"
                     /translation="MDRARSRQILLACASGLLLPFCFPRFDLGLLAWIILIPLHLALD
                     QCSRQRAFWIGWLSGLVGFTGIMAWVVTAMTTYGKVPEPVSYAILLLLATYLGLYVGL
                     YCLAVVWLRELIPRYGIFFAPCFWVALEWFRTHLLSGLPWCLLGYSQYRELDLIQVAD
                     HTGVYGISFLIVLVNVAFAELFLWIMPFFRGFHPVQLPWELLTTAAACMVLSWFYSSA
                     VLSARDLGHHKPSVTIGVVQPNIDQAVKWDASFRDETMRRFDRLTAQLGTSADLVVWP
                     EAATPFIFEREKEYQLQLITLAERAQAPILLGSPALRFYPDRRPYLLNSAYLLGSDGT
                     VLGRYDKHHLVPFGEYIPLKSSLLFFLDKLVEGIGDFEAGPGPTTLSFNPKSWSKERS
                     AGSRSVKFGVAICYEVIFPDLVRQFAMNGAEFLVTITNDGWFGPSSAPAQHFAMVVFR
                     SVENHLAFARAANTGISGFVDPYGQIIEATPIFTEEASSATIQTRQARTFYSYYGDVF
                     AHACVIICALLILFGYFRTKEPALTAITPA"
     gene            4329..5475
                     /gene="prfB"
                     /locus_tag="VN647_07540"
     CDS             join(4329..4397,4399..5475)
                     /gene="prfB"
                     /locus_tag="VN647_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWG96921.1"
                     /translation="MLEDVEVRVRALAEQVSELRGHLDFAHMTADLQELEAQMGQPHF
                     WNDARTAATVSRKKATIERELQQWRDIETKMGDLDALLELAHESGDSALETELAGELN
                     QLEPRLATLRVELLLSGELDPNNAIVAIHPGAGGTESQDWAQMLLRMYVRWAEHKKFK
                     VETLDLLPGDEAGIKSVTFSITGPYAYGYLKAEAGVHRLVRISPFDSNKRRHTSFASV
                     FVYPELSEDIDVVVEEKDLRIDTFRSGGAGGQNVNKVETAIRITHLPSGIVVQCQNER
                     SQLQNRNGAMKILKARLFELEQKKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQLVKD
                     HRTGHQISNVSSVMDGGLDGFIEAFLKKKLGKGSDHLAPVGGPEDDL"
     gene            5496..6980
                     /gene="lysS"
                     /locus_tag="VN647_07545"
     CDS             5496..6980
                     /gene="lysS"
                     /locus_tag="VN647_07545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWG96922.1"
                     /translation="MDELNEQRQQRIKKLDLLREAGVAPYGRRFEAKDRAGQLLTLHG
                     EKTKETLEQEKVPCTVAGRIVALRRFGKAGFAVLQDGSDRLQVYLKKDLLSEQAYTVV
                     EQLDLGDWIGVSGTLFRTKTNEFTVEVHHLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPLVHQIFSARSRIITGIRTFLIEREFLEVETPMMHPIPGGANAKPFVTHHNA
                     LGVDLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADY
                     QDLIVLTEELVSSLAQQILGKTVIPYQGKEIVLTPPWRRWSYHQAVLEVNNLDPSVVH
                     DREKALAAAKRLNVPVDPKASLFAIINEIFEETVEPNLQQPTFITDYPIEISPLAKRK
                     ETDPNLTDRFELYIAGREIANAFSELNDPLDQRERLEGQAAHRVAGDEEAHLVDEDFL
                     RALEFGMPPTAGEGIGIDRLIMLFTDQASIRDVVLFPQLRPEKS"
     gene            6980..8257
                     /locus_tag="VN647_07550"
     CDS             6980..8257
                     /locus_tag="VN647_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWG96923.1"
                     /translation="MALPYEIFVGLRYLRAKRRNRTISLNTFVSVAGITLGVAALIGT
                     VGIMTGFREDIQSKILGTTAHIVVQERMKEHMTDYDRLTDKIQTVPDVVAATPFVFRQ
                     VLLTTQSGVQGIILRGIDPKREANVTELAKNITAGQLLDLLAPVKVMQSPADDPQGDL
                     RPVEKPGIILGKELALRLGVFVGDTVNVVSPVGPISAMGMVPKIRTFAVVALFHSGMF
                     EYDSSFAYIELGEAQKFFNLGASVTGIEVKVTDVFRAGEIAHNIEQELGFSHGARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAMGATKR
                     SIRRIFMLNGLIIGFAGTGIGIPLGYAFLWLIQTFWTFDPSVYYISTIPVHVLAEDVL
                     LVAGSAILISFLATVYPANQAAKLEPVAALRYE"
     gene            8350..9012
                     /locus_tag="VN647_07555"
     CDS             8350..9012
                     /locus_tag="VN647_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG96924.1"
                     /translation="MIKVTNLHKSFSMGSYELPVLRGINLKIQRGELVAIVGASGAGK
                     STLLHIIGTLDKPSSGTVTFDEHDLFRMTDTQQAEFRNRRIGFVFQFHHLLAEFTALE
                     NACMPALVQRREPTSVKADATTLLTEVGLGQRLHHKPGELSGGEQQRVAMARALMQNP
                     DLVLADEPTGNLDTHSGDGLFGLMRTLNKTRGTTFVIVTHNDKLSAQSDRIIHMQDGQ
                     II"
     gene            9197..>10270
                     /gene="traF"
                     /locus_tag="VN647_07560"
     CDS             9197..>10270
                     /gene="traF"
                     /locus_tag="VN647_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HWG96925.1"
                     /translation="MRRSTLLYTAVLLVASTLPPHVSASEFVIAGPRAMGMGGAGVAV
                     TTNALATYWNPAGLAMTQTVDIRAQGGGQATDRLGLGDALHDLENFNTGDTSPANLTK
                     GQSIADRINRSGAAVSVNGSAGLYVKGHLGEHAFGFNVSDVATGGGFVSTPVQASQPG
                     GPGTPITVAGQMALRGLEARQVAFSYAYAFSDKTFSIGITAKIIQGAAYNGSTDLQGG
                     SGVSTIDHFGKPNISTTYGIDIGAIYRPSSWIRFGIVAKDINKPTFDAADGGDLKLEP
                     QVRAGIAINPYSSLTLTADVDATSNKTFVPGVKSQLLSLGVEQTILSEFLSFRAGTFK
                     NMQDASTPFVPTAGLGIRWFSFRA"
BASE COUNT         2217 a         2955 c         2787 g         2311 t
ORIGIN      
        1 gcagtcacag cgaacgctcg acctcgaccg catggtcgtg gcccagggcg tttcctttcg
       61 tgtactccat ctactaagcc gctcagcgca agtcatggtg ttactgggct ttcaatacaa
      121 tcgtcccttc catcgggatt ctgcatttct cctattgtta gcccttggcc cgatagtatg
      181 gatttccatg atcgaggttt ttccatttca accactgcct cggcatacaa tatggtcacc
      241 ggcttttctc tccatcgccc tatggcagcc tctgttcgaa gaattgctct tccgtggcat
      301 tgtccagggt cgtttgttgc aatccacggc gaggcggaag acatggattg gtctatccgt
      361 ctccaacctc ctgacatcgc tcctgttctc attggcccat ctggcgagcc attccgtttc
      421 gtggtccttg ctcgtgttcg ttccttctct ctgtttcggc ttcgtacgag atcgctttgg
      481 atctgtgcat ccctccatca ctctgcatgc cttctataat gccggttact ttcttctcct
      541 cggtggcctg actcttccaa actcctttta ggatgctcac cattagaacc gcgcttgcag
      601 gccatcgtgg aggaaccttc atgattgtca ggggccattt gcttgaacag gcttggaagg
      661 ctaagacaca aaatcactca gccatcgaga tgcttgtcga cggtatggta aagacttcgt
      721 tcagatcgct gaaggaacct ccgggagtcg gtccgccact gatgacataa atgcgccccc
      781 gtactactgc cgacccgata ccatgtcgag cagtcggcat cggagccata cgctgccagg
      841 tatcgctggc cgggtcataa acctcgttct cactgaatgt gccgctagac ccttcgccgc
      901 cgaatacgta gatccgtcct tctaccgcag cggctgtgat accgctccgc gcggttggaa
      961 gatccgcgac gcgcctccag cgatcagttg ccggatcgta ctgttccatg acagcaagat
     1021 tccgatggta gtcaccgtcg atgcggccgc cgatggcata aatcttcccg gcgaccgtcg
     1081 cggtgccgag atgatcgcgg gaggttggta gagatgccgc tgttgtccag acgttgcgca
     1141 cgggatcgta cacctcaacc tgcacattgt tggccttcct gtcgtagccg ccgatggcat
     1201 agagctttcc ctcatgttcg gtcacagcca atgcaccacg tgcagtcggc atcggagcgc
     1261 gaccggtcca ggtgtccgtg acaggatcgt aggcatatac cgtcgcgacc gggctccaga
     1321 cgctgagtcc cgatcgactg tatccgccta tgacgtacaa tcgtccatcc acgacaccga
     1381 ttcccacatg gtgcaagccg accggcatag gcgttttggg cgtccatcgg tccgtcactg
     1441 gatcatacat ttcaacggac ggcgtgatcg cgaaattcag caaattgccc acaccgggct
     1501 tctcaaaccc tccaacgacg tagatcttcc cctccagcat cgccgcagcc acctccgtcc
     1561 gtttcgtcgg cagcggcgct gctgcatgcc atgtgcctcg atcctgttcg agttgcgctg
     1621 attgagttgg tgctgtcggc gcgatcccca aaaacccagc aaagagcggt gacaataacg
     1681 aaatcagttt caccacaaag cgagttcctc cggctgatga tctcgtgaag aaggacatgg
     1741 tttcattcta tacctgctta aggtccagta gcatccggtt gaggacatag ctcatcatcc
     1801 ccattgcaag ggtcacaaaa tccgcaatcg tctctcggta gccgtgggca tcacatcaga
     1861 aagtcgatca tcgcgatcgg ccactgaaga gaatgactta tcacaggcgc tcgaataatt
     1921 cagacttccc tgtgggtggc catcggtagt ctgaaaaact gttgataaag ctgtcgctgg
     1981 gtttgacgga attctgttca ggggtagcgt acacctcggt taggcaatag cagcactact
     2041 tgctctttgc tcggcagagc agacaaaaag agctgcctaa agcgaggccg ccgaggaagg
     2101 tcagaccgag gatgacgccg cgcagagaat tcgttgtccc cgcttggcga cggatatatc
     2161 ggcgggcttg ggaaccgacg gtcgcttcgc ctccaatcca gcggcgcggg tcatcggtcg
     2221 gggtctcagt tggattgctc acgagtctca tgtctcctcc cttcatcaag cgcgatgcat
     2281 tcatcgatca gctgctccaa ctcatccatt tcctgatgtt gcacgtccac aaaccgaacc
     2341 ccgaacgttc gatcgacggt ccattgtacg cgagcttgtt caatcagaat cggaatatca
     2401 tctcctggga caatcagctt gacggcaatc tgctttcctg gttggacctc aagagagctt
     2461 tccaccaggg cgccaccaag cgacagatcg aggacttctc cttccacttc atgcgaattt
     2521 gcccgaagca agctcttgac ttgagccggc actcgacggt gacgacgtcg ctccatggaa
     2581 gatagaatac ctttttcacc ggggaaaagc acctggccgg tctcccaagt cgctgattgt
     2641 tcaagcgttc tcttgcaagt catcgaatct tttcgataag gtacggcacg atggaccgag
     2701 ctcgttcgcg ccagatcctc ctcgcctgcg cgagcggact gctgttgcct ttctgttttc
     2761 cacggtttga cctgggattg ctggcctgga tcatcctcat tccccttcat cttgcgcttg
     2821 atcaatgctc cagacagcgg gccttttgga tcggctggtt gagtggtctg gtcggattca
     2881 ccggcatcat ggcctgggtc gtcaccgcca tgacgaccta tggcaaggtc cccgaacccg
     2941 tcagttacgc aattttgttg ctgttggcca cctacctcgg actctatgtc gggctctatt
     3001 gcctcgctgt tgtatggttg cgcgagctca tcccacgcta tggaattttc tttgccccct
     3061 gcttctgggt cgcactcgaa tggttccgta cccacctgct ctccggcctt ccatggtgtc
     3121 tcctcggcta ttcacaatat cgcgaattgg acctgattca agtggccgac catacgggtg
     3181 tctacggcat ttccttcctc atcgtcctgg taaatgtagc cttcgccgag ctattcttgt
     3241 ggatcatgcc gttttttcga ggatttcacc cggtccagct tccgtgggaa ttgctcacca
     3301 ccgcggccgc gtgcatggtg ctctcatggt tctacagttc agcagtcctg agtgctagag
     3361 atctgggcca tcataaacct tccgttacca tcggtgtcgt tcagccgaat atcgatcaag
     3421 ccgtgaagtg ggatgcgtca ttccgcgacg agacgatgcg acggttcgac cggctgacgg
     3481 cccaactggg aaccagtgcc gatttggtcg tgtggcccga ggcagccacc ccgtttattt
     3541 tcgagcggga gaaagagtat cagctgcagc tgatcacttt ggcggaacgc gcgcaggcgc
     3601 cgatcctcct cggcagcccg gccttgagat tctatcctga tcgccgcccc tacctcttga
     3661 atagcgccta tctgctggga agtgacggca cagtcctcgg ccgttatgat aagcaccatc
     3721 tcgtgccgtt tggagagtac atccccctca aatcctcgct cctgttcttc ctcgataaac
     3781 tcgtcgaagg gatcggcgat ttcgaagcag gacctgggcc gacgactctt tcgttcaacc
     3841 cgaaatcctg gagcaaagaa cgttcggcag gctccagatc ggtcaagttt ggcgtggcga
     3901 tctgctacga agtgatcttt ccggatttgg tccgtcagtt cgccatgaac ggcgcggagt
     3961 ttctggtcac gatcacgaat gacggatggt ttggaccatc ctcggccccc gcccaacact
     4021 ttgccatggt cgtctttcgc tcggtggaaa atcatttggc cttcgcgcgt gccgccaaca
     4081 cgggaatctc cggcttcgtc gatccgtacg ggcaaatcat cgaagcgacg ccgattttta
     4141 cggaggaagc gtcctctgca acgatccaaa ccagacaagc ccgcacgttt tacagctatt
     4201 acggcgatgt gtttgcccac gcctgtgtta taatctgcgc gcttttgatt ctgttcgggt
     4261 acttccgcac caaggaacca gccctgacgg caatcacacc ggcatgaacg aagaaggagg
     4321 accgtgacat gttagaggac gtggaagttc gggtgcgtgc cctggcggaa caagtctctg
     4381 aactacgggg gcatctttga cttcgctcat atgacggccg acttgcaaga gctcgaagcg
     4441 cagatgggcc agcctcactt ctggaacgat gcccgcaccg ccgcaacggt gagccggaag
     4501 aaggcgacga tcgagcgaga gctccaacag tggcgcgaca tcgagaccaa aatgggtgat
     4561 ttggatgcgc tgctggagct tgcccatgag agcggcgact cggctctgga aaccgagctg
     4621 gccggtgaac tgaatcagct ggagcctcgc ctcgccacac tgcgtgtcga gcttctcctc
     4681 tcgggagaac tggaccccaa taatgccatt gtggcgattc atcccggtgc cggcggcacg
     4741 gaatcgcagg attgggcaca aatgctcctt cggatgtatg tgcggtgggc ggaacacaaa
     4801 aaattcaagg tggaaaccct ggatctgttg cccggcgacg aagcgggcat caaaagcgtg
     4861 acgttctcca tcacggggcc ctatgcctat ggatacctga aagcggaagc cggggtacat
     4921 cgtttggtgc gcatttcccc cttcgactcc aacaagcggc gacacacgtc gttcgcatca
     4981 gtcttcgtgt atccggagct gagcgaagac atcgatgtcg tggtcgagga aaaagatctc
     5041 cggatcgaca cctttcggtc gggcggcgca ggaggtcaga atgtcaataa ggtggaaacc
     5101 gccatccgca tcacgcactt gccgagtggt atcgtcgtgc aatgccagaa tgagcggtcc
     5161 cagctccaga atcgaaacgg ggcgatgaaa atcttgaagg cgcgcctctt cgaattggaa
     5221 cagaagaaga aggaagcgga gttcaacgcc atcgtcggcg aaaagaagga catcgcatgg
     5281 ggcagccaaa ttcgatccta cgtgttccag ccttaccaac tggtcaagga tcaccgaacg
     5341 ggtcatcaaa tcagcaacgt ctcatccgtc atggacggag gcctcgatgg attcatcgag
     5401 gcctttttga agaagaaatt gggaaaagga agcgatcacc tcgcaccggt cggcggaccg
     5461 gaagacgacc tgtagcagga ttgccgaagg gtaagatgga cgaactgaac gaacagcggc
     5521 agcagcgaat caagaaactc gatctccttc gagaggccgg cgtcgccccc tacggcaggc
     5581 gcttcgaggc caaggatcgg gccggtcaat tactcacgct gcatggcgag aagaccaagg
     5641 agaccttgga gcaagaaaag gtcccctgca cagtcgcggg acggatcgtc gccttgcggc
     5701 gtttcggtaa ggccgggttt gccgtgctgc aggacgggtc cgaccgcctc caagtatatc
     5761 tcaagaagga tctgctctcc gaacaggcct acaccgtcgt ggagcagctt gatctcggcg
     5821 actggatcgg cgtcagcggg acattgtttc gtaccaaaac gaacgagttc acggtcgaag
     5881 tccatcacct gacgtttctc agtaaagctc tccggccgct ccccgaaaag tggcatgggc
     5941 tgacggacgt tgaaaccagg tatcggcaac gttatgtcga tctgattgcc aaccccctgg
     6001 tccatcagat tttttcggcc agaagccgta tcattaccgg catccgaacg tttctcattg
     6061 agcgggagtt cctcgaggtc gaaaccccga tgatgcatcc gattccagga ggtgccaacg
     6121 ccaagccctt cgtcacgcac cataacgcac tcggcgtgga tctttacttg cgtatcgcgc
     6181 cggagctgta tttgaaacgt ctgatcgtcg gcggttttcc gcgcgtgttc gagatcaacc
     6241 gtaacttccg gaatgagggt atctcgacga tccacaaccc tgaattcacg atgctggagt
     6301 tctacgtgtc atatgcggat tatcaggact tgatcgtgct cactgaagaa cttgtgtcga
     6361 gcttggctca acagattctt ggcaagacgg tcattccgta ccaaggaaaa gagatcgttc
     6421 tcacgcctcc atggcgacga tggtcttacc accaagccgt cctagaggtg aataacctcg
     6481 acccatcggt agtccacgat cgagagaagg cattggcggc cgccaaacgg ctgaatgtgc
     6541 cggtagatcc gaaggcatcg ttgttcgcga tcatcaatga gattttcgag gaaaccgttg
     6601 aaccgaattt gcaacagccc acttttatca ccgattaccc gatcgaaatt tctcccctcg
     6661 caaagcggaa ggagacggac ccgaacctga ccgatcgatt cgagctgtac attgccgggc
     6721 gcgaaatcgc gaatgccttt tccgaattga acgatccgtt ggaccaacgt gaacggttgg
     6781 aaggccaggc ggctcatcgg gtggcgggtg atgaagaagc ccatctcgtc gacgaggact
     6841 ttctccgcgc ccttgaattc ggaatgccgc cgaccgccgg agaagggatc gggatcgacc
     6901 gattgatcat gctgtttacc gatcaggcgt ccatccgcga cgtcgtgctt ttcccacagc
     6961 tcagaccaga gaaatcgtag tggctctccc ctatgaaatc ttcgtcggac ttcgctacct
     7021 gcgcgccaag cgccgtaacc ggaccatctc gctgaacacc ttcgtgtcgg ttgccgggat
     7081 tacgctgggg gtggccgcgc tgatcggtac ggtcggcatc atgaccggct tccgggagga
     7141 cattcagagc aagattctcg gcacgaccgc acatatcgtc gtccaagaac gaatgaaaga
     7201 acacatgacg gactatgacc gtctgaccga caagatccag acggtgcccg acgtcgtggc
     7261 agccacgccg tttgtattcc gccaagtatt gctcaccacg cagagcgggg tacaaggaat
     7321 catcctccgc gggattgatc caaaacgaga agccaacgtc accgaactcg ccaagaacat
     7381 cacggcggga cagctgctcg acctgctcgc cccggtgaaa gtcatgcagt ctccggccga
     7441 tgaccctcaa ggcgacctcc gccccgttga aaaacccggt atcattctcg gcaaggagct
     7501 ggcgctcagg ttgggcgtgt tcgtcggcga tacggttaat gtggtctctc ccgtcggccc
     7561 gatcagcgcg atggggatgg tgcccaagat caggacgttc gccgtggtcg ccttgttcca
     7621 ttcaggaatg ttcgagtacg attcttcatt tgcttatatc gaactcggcg aagcacagaa
     7681 gtttttcaac ctgggcgcga gtgtaaccgg aatcgaagtc aaggtcacgg atgtgttccg
     7741 ggccggagag atcgcccaca acattgaaca ggagctggga ttcagtcatg gagccagaga
     7801 ttggatgcag atgaaccgca atctcttctc ggccctgaag ctggagaaga cgatgatgtt
     7861 tctccttctt gtcttgatca cgatcgtggc ctcattcaac atcgtcagca ccttgacgat
     7921 gatcgtgacg gagaagcaga aagaaatcgc gatcctcaag gcgatgggcg caacgaagcg
     7981 cagtatcagg cgcatcttca tgctgaacgg attgatcatc ggatttgcgg gtacaggcat
     8041 cggcattccc ttgggctatg cgtttctctg gctgattcaa acattttgga cgttcgatcc
     8101 gagcgtgtac tacatctcca ccattcctgt tcacgtgctg gccgaggacg tgctcctcgt
     8161 ggccgggtcc gccattctga tcagcttttt ggcgacggtc tatccggcca atcaggccgc
     8221 gaagctggaa cccgtcgccg cgctgcgcta tgaatagatt cgcaggactg agggtcgagg
     8281 actgagccga gaattacatg cttaagtgcc gagtaacaag gactaaggac tgagattaag
     8341 aatatcggaa tgattaaggt cacgaacctc cataaatctt tttcgatggg atcctatgaa
     8401 ttgcccgtcc tcaggggaat caatctgaag attcagcgcg gcgagctggt ggcgatcgtg
     8461 ggagcttcag gagccggcaa gagcaccttg ctccatatta tcggaacgct cgataagccg
     8521 agcagtggta cggtgacgtt cgatgagcat gacctctttc ggatgacgga cacacagcag
     8581 gcggaattcc gaaatcggcg gatcgggttc gtgtttcaat ttcaccacct gcttgccgag
     8641 ttcaccgcat tggaaaatgc ctgcatgccg gcgctcgtac agcgccgcga accaacttcg
     8701 gtcaaggccg atgcaacaac cctgctcacg gaagtcggat tgggacagcg cctccatcac
     8761 aagccgggag agctctcagg cggcgagcaa caacgtgtcg cgatggcacg cgcgttgatg
     8821 cagaatccgg acttggtctt ggccgatgag ccgaccggaa atcttgacac ccatagcggg
     8881 gacggattgt tcggattgat gcgcaccttg aacaaaacac gcggaaccac tttcgtgatc
     8941 gtgacccata acgacaaact ctccgcccaa tccgaccgca tcattcatat gcaggatggg
     9001 caaatcattt gatcgaaccg tccactcttt atcagagcat cccgctcgta ccaattggtc
     9061 ccgatatttt ccgagcgtgc cttcaacctt ctcgccgcgc ggaggttcgg cctgcagctg
     9121 tgaggctatc tgcgattcct tgacgaacca tcattcgttc tctagactca aaaccttact
     9181 gaatcgggga caggacatgc gccggtcgac gttgctttac acagccgttt tgctggtcgc
     9241 atcaaccttg cctcctcacg tctcggcctc agaattcgtg atcgcaggcc ctcgtgccat
     9301 gggcatgggt ggcgccggcg tcgccgtcac aaccaatgca ctcgcgactt actggaaccc
     9361 cgcaggcctg gccatgaccc aaaccgtgga catccgcgcc caagggggcg gacaagcgac
     9421 tgatcgcctc ggcttgggtg atgcgctgca tgacctggag aacttcaaca ctggtgacac
     9481 gtctccggcc aatctgacga agggtcagtc gatcgccgat cgtatcaacc gatctggagc
     9541 cgctgtttcc gtcaacggat ccgccggcct gtacgtcaaa ggacaccttg gcgaacatgc
     9601 gttcgggttc aatgtgtccg acgtcgctac cggaggcgga ttcgtctcga cgccggtgca
     9661 ggcatcccaa ccgggcggac ctgggacacc cattaccgta gcgggccaga tggcgcttcg
     9721 tggcctcgaa gccagacaag tggccttctc ctatgcctat gccttttccg acaaaacctt
     9781 ttcaatcgga attaccgcga aaatcattca aggcgccgca tacaacggct ctaccgacct
     9841 ccaaggtggg agcggtgtca gcacgatcga tcatttcggc aaaccgaaca tctcaacgac
     9901 ctatggaatt gacataggag caatctatcg tccatcctcg tggatcaggt ttgggatcgt
     9961 cgcaaaggac atcaataagc ctaccttcga tgctgcggac ggaggggact tgaaattgga
    10021 accgcaagtt cgggccggca tcgcaatcaa tccgtactct tctctgacat tgacggccga
    10081 tgtggatgcg acctcgaaca agacctttgt accaggtgtg aagagccaac tcttgagtct
    10141 gggagtcgaa caaacgatct tgtctgaatt tctttcgttt agggccggca ccttcaaaaa
    10201 catgcaggat gcgtccactc cgttcgttcc taccgctggg ttaggcatac ggtggttttc
    10261 cttccgtgct
//
LOCUS       DATNDD010000313         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3502202, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000313 DATNDD010000000
VERSION     DATNDD010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502202"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..930)
                     /gene="pgeF"
                     /locus_tag="VN647_07565"
     CDS             complement(190..930)
                     /gene="pgeF"
                     /locus_tag="VN647_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWG96926.1"
                     /translation="MTSTSVITVPAFADTGSQVRHFFGTRRHAMGLGLEVGIPQKGIA
                     GASSSWTLSVKQVHGTEALVVDRTVTSTDRFVGGWDALVTDQPGVMVAVRTADCVPIL
                     MHDPKRRVVAAVHAGWRGAVAGIVPKTLALLESRFGSRLEHVRISIGPSAGVCCYEVD
                     EPVLDHLCQGFPDWDKVVRTRGDGKANLDLKALVKEQAQALGAAPKSITTVNLCTICH
                     EDLFFSYRRERKVNGTMVSAIGLPVRRG"
     gene            complement(972..>2140)
                     /gene="ftsZ"
                     /locus_tag="VN647_07570"
     CDS             complement(972..>2140)
                     /gene="ftsZ"
                     /locus_tag="VN647_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWG96927.1"
                     /translation="PVRIKVIGIGGGGCNAINTMITSGLARVDFIASNTDLQALDRSL
                     APYKIQLGPERTRGLGAGAKPEIGRDAALESKEHIRECLEGADMVFVTAGMGGGTGTG
                     AAPIVASIAREMGILTVGVVTRPFQYEGKRRNKHAEEGIRDLRRHVDTLLVIPNQRLL
                     GIVDKSTPLLEAFKVADDVLRQAIQGIADVITTTGHVNVDFADVRTVMSHTGRAVMGM
                     GISYGPNRAIEAAQKAMCSPLLEEGSVEGARGVLLNITGGPSMSLHEIEEAASIIQQT
                     ADPEANIIVGQVINPEMGEELIITVIATGFEREEDSAEATIGADRGMSRPAKPVPSVL
                     AGMGASLAVDRPMKDLDRPTFLRRMNDARESMDRAVLTAEDEWDVPTFLRKQTD"
BASE COUNT          431 a          686 c          551 g          472 t
ORIGIN      
        1 ccgcaccgta ccggcgggac gtccggcttt ttcttccgcc gaccgaattt tcgtgagcac
       61 cgactgaaca cgtttcgcta tcgcttccgg gatgagcggt tccatcgtcc cctcgcacga
      121 gtcgcggccg ctcattcagc gaaccgtcct tattctagcg gaagaacctc cgaattggca
      181 gagtctgact tatccacgcc tcaccggcaa cccgatggcg ctgaccatgg tgccgttgac
      241 tttcctctcc cgccgatagg agaaaaagag gtcttcatgg caaatcgtac agaggttgac
      301 ggtcgtgatg gacttcggag ccgccccaag ggcttgcgcc tgctctttga cgagcgcttt
      361 caaatcaaga ttcgcttttc cgtctcctct cgtccgtacc actttgtccc aatccggaaa
      421 tccctgacac agatggtcga gaaccggctc atccacttca tagcagcaaa ccccggcgga
      481 tgggccaata ctgatccgta cgtgctctaa acgcgagcca aagcgagact ccaacagtgc
      541 cagcgttttg gggacaatgc cggcaacagc tccccgccag ccggcatgga cggcggccac
      601 gacccgacgt ttaggatcat gcatcaggat cggaacacaa tctgccgtcc gcaccgctac
      661 catgactcca ggttgatcag tgaccaaggc atcccagccg cccacaaatc ggtccgtcga
      721 agtcacggta cggtccacca ccagcgcttc cgttccatgg acttgcttta ctgagagcgt
      781 ccatgatgac gatgcgcctg cgatcccctt ctgcgggatc cccacttcga ggccaagacc
      841 catggcatgc cggcgggtcc cgaaaaaatg gcggacctga ctcccggtat ccgcaaacgc
      901 cggcacggta atgaccgatg tgcttgtcat aatcaaggtc aatacctcat cacgtgaaaa
      961 tatgcacgag atcagtctgt ttgtttacga agaaatgtcg gcacatccca ctcatcctcg
     1021 gcagtcaaca ccgcccggtc catcgactct cgcgcatcat tcattcttct caaaaaggtg
     1081 gggcgatcga gatctttcat cgggcggtct accgccagtg acgcgcccat gccggccaac
     1141 actgatggaa cgggtttggc cggccgactc attcccctat cagctccaat cgtggcttca
     1201 gctgagtcct cttctcgttc aaatccggtc gcgatcaccg taatgataag ttcttcgccc
     1261 atctccgggt tgatgacctg accgacaatg atattggctt cgggatctgc cgtctgttgg
     1321 atgatggagg cggcttcctc gatctcgtgc agcgacatgc tggggcctcc cgtaatattc
     1381 aggaggacgc cgcgagcccc ttcgacactt ccctcctcaa gaagaggact gcacatggcc
     1441 ttttgagcgg cttcgatcgc ccgatttggt ccataggaaa tgcccatgcc catgaccgct
     1501 cgtccggtgt gtgacatcac ggtgcggaca tcggcaaaat cgacgttcac atgtccggtg
     1561 gtcgtaatca cgtcggcgat gccttggatc gcttgccgca acacgtcatc cgcgactttg
     1621 aacgcttcga gaagcggggt cgatttgtcg acaatcccca gcagccgctg gtttggaatg
     1681 accagcagtg tatcgacgtg ccgacgcaaa tcgcgaattc cttcttccgc gtgcttgttt
     1741 cgccgtttgc cttcatactg aaatggcctc gtgacgacgc ctaccgtgag gatacccatt
     1801 tcacgggcga tgctggccac aatgggggcg gctccggtac cggtccctcc tcccatccct
     1861 gccgtgacga aaaccatgtc cgccccttcg agacattctc ggatgtgctc tttactctca
     1921 agcgcggcat ctctgccgat ctccggcttt gcccccgcac ccaacccgcg ggttcgttcc
     1981 ggcccgagtt ggatcttata aggtgccaac gaccgatcaa gcgcctgtag gtcggtgtta
     2041 ctcgcaataa aatcgacacg cgcgagtccg gaggtgatca tcgtattgat cgcattgcac
     2101 ccgcctccgc cgattccgat caccttgatg cggaccggcg
//
LOCUS       DATNDD010000314         3392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1807742, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000314 DATNDD010000000
VERSION     DATNDD010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807742"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VN647_07575"
     CDS             <1..466
                     /locus_tag="VN647_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96928.1"
                     /translation="LLGLTKGHRSTSSANVYPETIYGDLVHRESKRSERSGHLCRILL
                     VYHTSEQGRVAPFGSELADKAISVLSIRCRDTDYIGWYRQGRILGILITTLRPDSVRD
                     GYDNLKARVVDSLRGILTFTDDHSLQIRMLELSELTAFNASDHSASSPGSKD"
     gene            497..1498
                     /locus_tag="VN647_07580"
     CDS             497..1498
                     /locus_tag="VN647_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWG96929.1"
                     /translation="MLRLGVIGYGYWGPNVVRNFLVHQDCKVTTICDKSSTALTRVLA
                     AHPGICVTTEPDAVLFATDIDAVAIITPVSYHYELAKKALENGKHVFVEKPFTATSVE
                     AEELIQLAECRGLQIMVDHTFLFTGVVRKIKQLINDGTLGRLYYYDSTRVNLGLFQHD
                     VNVLWDLAPHDLAIMDYLIGVEPELVVATGGAHVNNLENIAYLTVYFPDNVLAHINVN
                     WLSPVKMRTTLVGGQKRMLVWNDLDLAEKLRVYDKGADIKNESGMHAALVSYRTGDMY
                     APKIEEAEALKVETRYFLDCIMNGTKPINDGLAGLRVVRILEAAEQSLNQHKEIVLA"
     gene            1560..2651
                     /locus_tag="VN647_07585"
     CDS             1560..2651
                     /locus_tag="VN647_07585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWG96930.1"
                     /translation="MTGQRPKLLDERLFKQAITRERKRADRSGLAMVLLLVGLPNGSG
                     EQGKADDAVVTNALSAVASEPDIVGWFESSHVIGLIVSEIDPVGLTGTCDRLESSVLS
                     AIALQGDEGLAQHLSIKLRVYPEPTVSNEKQVCPMDPLLYPELSATQPAVQNFWILKR
                     GMDIVLSALLLILLFPVFSLIAAIVKLSSPGPVLFRQMRVGHLMMPFTMCKFRTMYAA
                     VDHQVHHDYVSWFITASDQTQSQQKPPVFKLTNDTRITPIGRFLRRTSLDELPQLWNV
                     LVGDMSLVGPRPPLPYELQQYKPWHRGRVLEAKPGMTGLWQVVGRSRTTFDEMVRLDL
                     RYARTMSLWSDIKILLATPAAVITGKGAC"
     gene            2871..>3392
                     /locus_tag="VN647_07590"
     CDS             2871..>3392
                     /locus_tag="VN647_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HWG96931.1"
                     /translation="MQERKTLALRPNLVLGGIISIGLIICSFQGCSRDSSSSSIPSGS
                     PPVSAPLPVFPLVVSANHRYLQDQNGVPFPILGRTAWFITSLPETDYKRFIDDTVAKG
                     YNAIEFHVINHDRRGNHPPFGPNNTLPFTVKLDGSIWDGSLGGSNDQAPDLSQPSEVY
                     WENVDALLTYAESK"
BASE COUNT          749 a          918 c          930 g          795 t
ORIGIN      
        1 cttgcttggc ttgacgaagg gccataggtc tacctcttct gcaaatgttt atcctgaaac
       61 gatatatggc gacctcgtcc accgagaatc caagcgttcc gagcgctctg gtcatctttg
      121 tcggatcctg ctcgtctatc acaccagcga acaaggacga gtcgcgccct tcggatccga
      181 acttgccgac aaggccatct cggtattgtc catcagatgt cgcgacaccg attacatcgg
      241 gtggtatcga caaggccgca tcttagggat cttgataacg acgctacgac cggattctgt
      301 cagagatggg tacgacaatc tgaaagcccg tgtggtggac agtttgcgtg gcatcctgac
      361 tttcacggat gaccactctc ttcagatacg tatgcttgag ctgagtgaac tcacagcatt
      421 caatgcgtcc gaccattctg cttcgtctcc tggttccaaa gactagatcc tataacctct
      481 ttcaaggagt gcaatcatgc tacgactcgg tgtgatcggg tatgggtatt ggggcccgaa
      541 cgtggtgagg aatttcctcg tacatcagga ctgcaaagtc accactatct gcgacaagtc
      601 ttccacggca ttgactcgtg tgttggcggc tcatcccggc atctgtgtga cgactgagcc
      661 ggatgcggtg ctctttgcca cggacatcga tgcggtggca atcatcacgc ctgtatcgta
      721 ccactatgaa ctcgccaaga aagcgctgga gaatgggaag cacgtgttcg tggagaaacc
      781 ctttaccgca acctccgtcg aggctgagga gctgatccaa cttgcggagt gccggggact
      841 ccagatcatg gtcgaccata cctttctgtt taccggagtt gtccgcaaga tcaagcaact
      901 tatcaacgac ggcacattgg gccgcctcta ctactatgat tcgacacgag tgaatctggg
      961 gctcttccaa cacgacgtga acgtactctg ggatctcgcg ccgcacgact tggcgattat
     1021 ggattacctg atcggggtcg aacccgagtt ggtcgtggct accggcggag cccacgtgaa
     1081 caatctcgaa aacatcgcct atctcaccgt gtatttcccc gataatgtcc tggcgcacat
     1141 caacgtcaat tggctctcgc ccgtgaaaat gcgcacgacg ctggtcggcg gccaaaagcg
     1201 aatgctggtt tggaatgacc tcgacctagc ggaaaagctc cgcgtctatg ataagggggc
     1261 cgacatcaag aatgaatcgg gaatgcatgc agccttggtc agctacagga ccggtgatat
     1321 gtacgcgccg aaaatcgaag aggccgaagc gctgaaggtg gagactcgct atttcctgga
     1381 ctgcatcatg aacgggacca aaccgattaa tgatggtctg gcgggactac gagtcgtgcg
     1441 catcctcgaa gcggccgaac aatcgctcaa tcagcacaaa gagattgtat tggcctaggc
     1501 ttcatttttt gggacggagg atgaaggatg aggggacaat cgcacacgag gccgttggca
     1561 tgacggggca acgcccgaag ctgctcgacg aacggctctt caaacaagcg atcacgcgag
     1621 aacggaagcg tgcggatcgc tcagggctgg ccatggtcct gttgttggtc ggccttccga
     1681 acgggtcagg agagcagggg aaggccgacg acgctgtcgt gacgaacgct ttgtcggcgg
     1741 tcgcctcgga gccagatatt gtaggttggt ttgaatcgtc gcatgtcatc ggtctgattg
     1801 tgtccgagat cgaccccgtc ggcttgaccg gaacctgcga tcggctggaa agctcagttt
     1861 tgagcgcgat cgcacttcag ggcgatgagg ggcttgcgca acatctttcg atcaagttgc
     1921 gtgtgtatcc agagccgacc gtatcaaacg agaaacaggt gtgtcccatg gatccactgt
     1981 tgtatcccga actttcggcg acccagcctg ccgtccagaa tttttggatc ctcaaacgag
     2041 gcatggacat tgtgctcagt gctttgctgt tgatcctatt attcccggta ttctccctca
     2101 tcgctgcaat tgtcaagctg tcgtcgcccg gaccggtcct gttcaggcaa atgcgcgtcg
     2161 ggcatctgat gatgccattc acgatgtgca agttcagaac gatgtatgcc gcggtggatc
     2221 accaggtcca ccacgactat gtgagctggt tcatcaccgc cagcgaccag acgcaatcac
     2281 aacagaagcc tcccgtcttc aagctcacga acgataccag aatcactccc atcggccggt
     2341 ttctccgaag aacgagtctc gatgagctgc cccaactctg gaatgtgttg gtcggtgata
     2401 tgtccttggt ggggccacgg ccgccgcttc cgtatgaact gcaacaatac aagccatggc
     2461 acagaggtcg cgtgttggag gccaagccgg gcatgaccgg cctctggcaa gtcgtcggtc
     2521 ggagtcggac caccttcgac gagatggtgc gcctcgactt acgatatgcc aggaccatgt
     2581 ccctatggtc cgacatcaag atcctgctcg cgacgcctgc ggcggtcatt accggcaagg
     2641 gggcttgctg atacgatctg tctcctctgg aatttttgtt tttaccagaa gcacgatcct
     2701 ggttttcctt acctgtgccc tccgctattg cctgtccagg gcgatattgg atcaaagact
     2761 gaagcaactc gtcttctgcg acacgccctt cgtgtgaagt ttcagttttg aggccttgtt
     2821 atgtcggagg ggaagtgcgg tgggtgaatt tgtggagggt tgtcggcttc atgcaagagc
     2881 gaaagacact ggctttgagg cctaatctag tgctgggcgg catcattagc atagggttga
     2941 ttatttgcag ctttcaagga tgcagcaggg attcgtcatc ttcctcaata ccatctggtt
     3001 caccaccagt ctccgcgcct ctacccgttt tcccactcgt cgtatctgcc aatcatcgat
     3061 atttgcagga tcagaacggc gtgcctttcc ctattctagg gcgcacggcc tggttcatca
     3121 catcgctccc ggagacagac tacaaacgat ttatcgatga cacggtagcc aaaggctata
     3181 acgctatcga atttcacgtc attaaccacg ataggcgagg caatcacccg ccctttggcc
     3241 caaataatac gcttccattt acagtgaagc tcgacggctc gatctgggat ggttctcttg
     3301 ggggtagcaa tgaccaggcg cctgatttgt cacagccgag tgaggtctac tgggaaaacg
     3361 tcgatgccct cctgacatat gccgagtcca ag
//
LOCUS       DATNDD010000315         7926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4075202, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000315 DATNDD010000000
VERSION     DATNDD010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4075202"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VN647_07595"
     CDS             <1..836
                     /locus_tag="VN647_07595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG96932.1"
                     /translation="VYQDPAAIMLRSRLGVEESEGSTGSGADSSPHHHGMMSTPRLKD
                     KVAIVTGSNSGIGKAIALRFGREGAKVIVAARRLQLCEQTVSQVKMQGGEAWPIQTDV
                     ADEQQVERLIADTMTRYGRIDILVNNAGIGGGRRLAETSTETFDQVMSVNLRGTFFCC
                     RAGFRQMKQQGGGVIINMSSVAGLQAWAGTGTYSASKHGIMALTQSLADEGRPYHIKV
                     SAICPAAVADELVDASEAEIERSEKIDPFDVAEAALYLSTLGKYAVVHHIAIDRLGAE
                     W"
     gene            complement(1000..1485)
                     /locus_tag="VN647_07600"
     CDS             complement(1000..1485)
                     /locus_tag="VN647_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96933.1"
                     /translation="MLAGRLVKLIEKNSEQLAHDLSEKVWNSPRCSDLRKVPPNELQA
                     RTREIYQNLNNWLMDMTEAEIERRYTELGARRASQGVAYSHFLWAITATRDHMVAFVQ
                     REGLSDSAVELHGELELMHVLGKFFDRALYYTALGYEGERTRIGTNLRRRRDDHQKVH
                     A"
     gene            1858..3411
                     /gene="purH"
                     /locus_tag="VN647_07605"
     CDS             1858..3411
                     /gene="purH"
                     /locus_tag="VN647_07605"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWG96934.1"
                     /translation="MAGIKRALISVSDKTGVIEMAKALEALGAEILSTGGTAKTLRDA
                     GVKVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRSLPAHVDQMNQHGIGPIDVVVV
                     NLYPFEATIAKPDCRFDEAIEHIDIGGPSMLRSAAKNHDDVLVVVDPADYSRVLEAVT
                     GNTVTPALRRELAMKVFQHTSRYDGLIAGYLEKHANGGEIKFPKVLSLQFELAESLRY
                     GENPHQQGAFYRELDSKEPSVSRGKILHGKAMSYNNFLDANSALELIKEYGETAVAII
                     KHNNPCGVALGETPTEAYVKARETDPVSAFGGVIAFNRPVDMTAAKEITSTFVEVVIA
                     PGFAEDALAELKRKKDLRLLDVGPLMKVKQEGFDLKKLVGGLIVQDRDLGVLSDLRTL
                     AVPTIRKPTDEEYAACAFAWKVCKHVKSNAIIYAKPGQTVGIGAGQMSRVDSVKLAAM
                     KAQMPVKGCVMASDAFFPFRDGLDAAAEVGITTVIQPGGSIRDAEVVKAADEHGMAMI
                     LTGMRHFRH"
     gene            3563..4837
                     /gene="purD"
                     /locus_tag="VN647_07610"
     CDS             3563..4837
                     /gene="purD"
                     /locus_tag="VN647_07610"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWG96935.1"
                     /translation="MKILVVGSGGREHAMVWKLAQSPRKPLLYCAPGNAGIASLAECI
                     PIKSDDVAGLKDFALREKVDVTVIGPEAPLALGIVDEFRRARLRVFGPTKSAARLEAS
                     KIFSKEVMARAKIRTAHAESFEKIAAALAYVERQELPIVIKADGLAQGKGVIIATARQ
                     QAQQAIVDSMEKAVFGQAGKQVLVEQFLDGEELTIMAFTDGKTVVPMPPAQDHKRVGD
                     GDTGLNTGGMGAYSPAPLGTSELQDKVLHEVLQPMVDAMTRLGSPFQGVLYAGLMVVN
                     GTPYVLEFNARMGDPETQVVLPLLKTDFLDVIESVVEHRLDQLPVEWHPDATVCVVMA
                     SEGYPGSYRQGMPLSGLPPATISSDSSMVFHAGTAQKGNEIVTAGGRVLGVLGRGRTL
                     SEAQREAYRAVKTISFEGCHFRTDIAHRALKT"
     gene            5078..5509
                     /locus_tag="VN647_07615"
     CDS             5078..5509
                     /locus_tag="VN647_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96936.1"
                     /translation="MNRHPTQLVSTVVGWGLILGLAGCDYWPPALQAEIEQLRSEIQT
                     LTMEKTQLQAQVTDLSKTKQELQGQIDELSYINRGKTEMITGLQHQLDTARARMLKAM
                     APKTPFHKIPVKPAAKARPASKSTPKAPANKSHTPRAVDVR"
     gene            complement(5927..7120)
                     /locus_tag="VN647_07620"
     CDS             complement(5927..7120)
                     /locus_tag="VN647_07620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWG96937.1"
                     /translation="MKLAARVGRIAPSPTLAMAATAKAMAAQGLDVIDFSAGEPDFDT
                     PDPVKAAAEAAIREGFTKYTPSSGIDELRNAIADKLHAELGLRYEKSQILVSCGAKHS
                     LYNLAEALLEAGDEIIIPIPYWVSYSDQTLLNDATPVLLPTSEEDGYAVHASELERLI
                     TPRTKAIIVNSPCNPTGTTYDRATLEQIASAAVRHDLLVISDEIYEKVLYDGARHISI
                     ASLGPEIAARTVIINGVSKSYAMTGWRIGYAAGPKDLVTAMANIQSQSTSNPCSISQK
                     AAVAALRLGEPFTRKMVEEFSRRRIAIVDGLNKIPGVSCRMPTGAFYAFPNIKGVLGK
                     RGPSAVLRAPNDVAHYLLHDARIAAVPGEPFGSQEHLRLSYATSMANITRGLERLEQA
                     FAKLL"
     gene            complement(7117..7605)
                     /gene="coaD"
                     /locus_tag="VN647_07625"
     CDS             complement(7117..7605)
                     /gene="coaD"
                     /locus_tag="VN647_07625"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWG96938.1"
                     /translation="MKIGIYPGTFDPITYGHADIITRSLRVFDKVVVAVAPNPSKHPL
                     FNLAERLDMITLVMKDVGQVEVTPFDGLLVDYVERSGAHAIIRGLRAISDFEHEFQMA
                     LVNRKLAKMVETVFLMPSEEYSYLSSTIIKDVAQHGGSVTDFLHPEVARRLQARIRSL
                     KS"
     gene            complement(7602..>7926)
                     /locus_tag="VN647_07630"
     CDS             complement(7602..>7926)
                     /locus_tag="VN647_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HWG96939.1"
                     /translation="NLELCHIGEEIAVLDQTVQQFLDHPDQWNGPYDIVFADPPYAEG
                     SELPSILAMPGADEWFALDSWLIVEHAVKTTLPMSLGCTKFLRRYRYGDTVLSLYSRS
                     HAVLA"
BASE COUNT         1794 a         2081 c         2309 g         1742 t
ORIGIN      
        1 gagtgtacca agatccggcg gcgattatgc tccgaagtcg tcttggggtc gaagaaagcg
       61 agggttcgac cggaagtggc gctgatagtt cgccacatca tcacggcatg atgagcacac
      121 cacgactgaa ggacaaagtt gcaattgtga cgggcagcaa cagcgggatc ggaaaggcca
      181 ttgctcttcg attcggtcga gaaggggcga aagtgattgt cgccgcgaga cggttgcagt
      241 tatgcgaaca aaccgtttcg caagtcaaga tgcagggtgg agaggcatgg cccatccaga
      301 ctgatgtagc cgatgagcag caggtcgagc gattgatagc cgacacgatg acccgctatg
      361 gccggatcga tattctcgtc aacaatgccg gtatcggagg cgggcgtcgc ttggctgaga
      421 cgagcaccga gacattcgat caggtaatga gcgtgaacct gcgcggcacg ttcttctgct
      481 gtcgagccgg ctttcgacaa atgaagcagc agggcggtgg agtgattatc aacatgtcga
      541 gcgtcgccgg tctgcaagcc tgggcaggca ctggaaccta cagcgcgtca aaacacggta
      601 ttatggcgct tactcaatcg ttagctgatg aaggccggcc ttatcacatc aaagtcagtg
      661 cgatctgtcc ggcggctgtg gcggatgagt tggtggatgc gtcggaagcg gagatcgagc
      721 gcagtgagaa aattgacccg ttcgatgtgg ccgaagcggc tctgtatctt tcgacacttg
      781 ggaagtatgc cgtcgtgcac cacatcgcca tcgatcggtt aggcgctgaa tggtgaagct
      841 catggcttga caaccggtct tctgtaccga tgacaatgac ggactccagg atgtcggtac
      901 ttctgtgtga acggttcggt tgaggtaggt aggtatgggc tcactcatgc agtgagcccg
      961 taggggcggt ggatgacaca aatttcgctc gcgcagatgc tacgcgtgca ctttctgatg
     1021 atcgtccctt cgcctgcgga ggttcgttcc gatgcgagtc cgctcgcctt catacccaag
     1081 ggctgtatag tataacgcgc ggtcaaaaaa cttaccgagg acgtgcatga gttccaactc
     1141 accgtggagc tcgacggcgc tatcggacaa cccttctcgc tggacaaagg ccaccatatg
     1201 gtctctggtc gcggtgatcg cccagaggaa atggctgtag gcgacccctt gggaggctcg
     1261 gcgtgcgccc agttccgtgt agcggcgctc gatttcggcc tccgtcatat ccatcaacca
     1321 attgttcaag ttctggtaga tctcacgggt gcgggcttgg agttcgtttg gtgggacctt
     1381 gcgcagatcg ctacagcgtg gagagttcca cactttctcg ctgagatcat gggcaagctg
     1441 ttcggagttt ttctcgatca gtttcaccaa ccggccagcc agcatagcgc acctccctag
     1501 tgagtggtta gttatgtagg ggaatgcatg gggactcctt actgccgatg agacattact
     1561 tgaatggtag taccaacgtc aatgacacgt ttgtcgggct gaggctggta aatgctgtac
     1621 tccacatcct ggtaaatgcc gtattcccat atcgatgtac gagaacatgt ccagtaatga
     1681 gagcgtcgga ttcaaccgtc tccaccgatc ccgtcgcaga caaaagaaag gtctattgag
     1741 atagaggcag tcggcccatg tcgccttgaa cttgcctcac ccatttgcta ttctttgcat
     1801 gcctttcgta gtcgacgtat ctcttccaca tcgcacacaa tcctaaggag tactcacatg
     1861 gccggcatta agcgggcgtt gatcagtgtt tcggataaga ccggagtcat cgagatggcg
     1921 aaggcacttg aagcgcttgg cgcggaaatt ttgtccacgg gaggaacggc caaaacgttg
     1981 cgcgatgcgg gggtgaaagt cacggatgtc gccgcctata cgggatcgcc ggagattctc
     2041 gacggtcgag tgaaaacgtt gcaccccaaa atccatggcg gcttgctggg gcgccggtcg
     2101 cttccggcgc atgtcgatca gatgaatcaa catgggatcg gtccaatcga tgtagtcgtg
     2161 gtcaacctct atccctttga agccacaatc gccaaacccg actgccgttt cgatgaggcc
     2221 attgaacata tcgatatcgg tggtccgtcc atgctgcggt cggcggccaa gaatcatgac
     2281 gatgtgttgg tcgtggtcga tccggccgat tactcgcggg tgctggaagc cgtgaccggc
     2341 aatacagtga caccggcgct gcgccgtgaa ttggcgatga aggtttttca gcatacgtcg
     2401 cgttatgacg gattgatcgc gggctatttg gagaagcatg cgaacggcgg ggagatcaag
     2461 ttcccgaaag tattgtcgct tcagtttgag ttggcggaat ccctccgcta cggagagaat
     2521 cctcaccagc aaggcgcgtt ctatcgtgaa ttggacagca aggagccatc ggtttctcgt
     2581 gggaagatct tgcacggcaa agcgatgtct tacaataact tccttgatgc gaactccgct
     2641 ctcgagctga taaaggaata tggagagacg gccgtggcga tcatcaaaca caacaatccc
     2701 tgtggtgtgg cgctcggtga aacgcccacg gaggcgtacg tcaaggcgag agagacggat
     2761 cccgtgtccg ccttcggcgg ggtgattgcc ttcaaccggc cggtggatat gacggctgcc
     2821 aaggaaatca cgtccacgtt tgttgaggtc gtgatcgctc cagggtttgc tgaagatgca
     2881 ttagccgaac tgaagcggaa gaaggatctg cgtttattgg acgtgggccc cttgatgaag
     2941 gtgaagcagg aagggttcga tctgaagaaa cttgtcggcg ggctcatcgt acaggatcgc
     3001 gatctcggtg tcttatcgga tcttcggacg cttgccgtgc cgaccatccg taagccgact
     3061 gatgaagaat acgccgcttg tgcgtttgcg tggaaagtct gcaaacacgt taagtccaac
     3121 gccatcattt atgccaagcc cggccagacc gtcggcatcg gggcagggca gatgagccgt
     3181 gtggattccg tgaagttggc ggccatgaaa gcgcaaatgc cggtcaaagg ttgtgtcatg
     3241 gcttcggacg ccttctttcc gttccgcgat ggcctcgatg ccgcagccga agtcggtatt
     3301 acgaccgtca ttcaacccgg cggatcaatc cgggatgcag aagtcgtcaa agcagcggat
     3361 gaacatggga tggcgatgat tctcacaggc atgcggcatt tccgccattg atcggcagct
     3421 gaaaatggcc tgtcgctgcg ttctcggtcg cccgtctacc tcaacgtacc ggaaaacgta
     3481 cgcctcggtg tccgagcttc ctgcggcctt gcgacaggcc attttgagca gcctctttgt
     3541 ctttcctacc taaagaaaat ctatgaaaat tcttgtcgtc ggcagcgggg gacgcgagca
     3601 tgcgatggtg tggaagctcg cgcagagtcc acgcaagccc ctactgtatt gtgcccccgg
     3661 caatgcaggc attgcgtcac tggcggaatg catcccaatt aagtcggacg acgtggccgg
     3721 tctgaaagac ttcgctcttc gagaaaaggt tgatgtgacg gtgatcgggc ctgaagcgcc
     3781 tctcgcattg ggcatcgttg acgaattccg cagagctcgg ctcagagtgt ttgggccgac
     3841 caaaagcgct gctcgtttgg aagccagcaa gatcttctcg aaggaagtca tggcacgagc
     3901 aaagatccgg accgctcacg ccgagagttt cgaaaagatc gctgccgccc tggcctatgt
     3961 cgaacggcag gagttgccga tcgtgatcaa agcggacggg ctggctcagg gaaaaggcgt
     4021 catcattgcc accgctcgtc agcaagcaca gcaggcgatc gtggattcga tggaaaaggc
     4081 cgtgtttggt caagccggta aacaggtgct ggtcgagcag tttctcgacg gcgaggaact
     4141 gacgattatg gcgtttaccg acggtaagac ggtggtaccg atgccgccgg cgcaggatca
     4201 taagcgggtc ggtgatggcg acacaggctt gaataccggt ggaatgggag cctatagtcc
     4261 agcccccctt gggacatctg agctccaaga taaagtttta catgaggtct tgcagccgat
     4321 ggtagatgcc atgacacggc tcggttcacc attccaaggt gtcctatatg caggactgat
     4381 ggtggtgaat ggaacgccgt acgtgctcga attcaacgct cgtatgggag atccagaaac
     4441 acaagtggtg ctgcccttgc tcaagaccga ttttctcgac gtcatcgaat cggtggtcga
     4501 acatcgactg gatcaacttc ccgttgagtg gcatccggac gcgacggtct gcgtggtgat
     4561 ggcatcggag ggctatcccg gctcctatcg acaggggatg ccgctttctg gactccctcc
     4621 agcaaccatt tcgtcggact cctcgatggt ctttcatgcg ggaactgcac agaagggcaa
     4681 tgagatcgtg acagccggcg gtcgagtcct cggcgttctc ggtcgaggac gcacgttatc
     4741 cgaggcacaa cgcgaagcct atcgagccgt gaagacgatc tcctttgagg gttgccactt
     4801 ccggacggac attgcgcacc gggctctgaa gacatagtca gccatcctcg agccaccgat
     4861 tcgacgacga tgtcgatcat atcccatctg ctgctgagat agatctacct acagaagcag
     4921 gcgtaggtcc gaatagccgg ggccgatctt ctttttagcc gattcgtgcg atctgttaga
     4981 atacttttcc atcacggtca gtgtacccac gtggcaaaga cacaggaagc ccatacactg
     5041 gtgtggaacc atcatgtctg atgaaagggg aagggccatg aaccgccacc cgacgcagtt
     5101 agtcagtacg gtagttggtt ggggcctcat acttggcctt gcggggtgtg attattggcc
     5161 gccggccttg caagctgaga tcgaacaatt gcggtcagag atccaaacct tgaccatgga
     5221 gaagactcag cttcaggcac aggtcaccga cttgtccaag accaaacagg agctacaggg
     5281 acagatcgat gaactgagct acatcaaccg gggaaaaacc gaaatgatta ccggcctcca
     5341 acatcaattg gatacggcac gcgcgagaat gttgaaggcg atggctccga agacaccgtt
     5401 tcataaaata ccggtgaaac cggcggcgaa ggccaggccg gcctctaaaa gcacccccaa
     5461 agcgccggca aacaaaagcc acactccgag ggcagtggat gtccgataat cggcccggga
     5521 atctatgaag aggagggcgt cgtcgtattc gatgaagatg ttgacgagct tgcagatgcc
     5581 gctggcgtcg cggcgggagc cgaagatgtt gaaggtgtcg gcgctgaagg ggctgactcc
     5641 ttcttctctt ccgtcttggc accggcctca ccacccgcac tcgacggcgg tttgagtttg
     5701 tccgagtagt cggtcacgta ccagccgctc cccttaaaca tgatcccagg cgatgaaatc
     5761 agccgtctga ccgcgctccc acaccgttca cacgtcgaga gcggatcatc tttgatgctc
     5821 tgcttcactt caaaacgata cgagcaactg tcgcactgat attcgtaaat cggcaccgca
     5881 ttcctccctc gttaccttgc atagagctcg atgagacaat ccttccttac agcaacttcg
     5941 cgaacgcttg ctccagacgc tcaagtcctc gggtaatgtt cgccatactg gtggcatagg
     6001 acagccggag atgttcctgg cttccaaagg gttcaccggg aacggccgcg attcgggcat
     6061 catgcaagag ataatgcgcc acgtcattcg gagccctgag tacagccgag ggaccacgtt
     6121 tccccaacac gcctttgata ttcggaaatg catagaaagc gccggtcggc attcggcatg
     6181 acacacccgg aatcttgttc aatccgtcca caatggcaat ccggcgacgg gaaaattctt
     6241 ccaccatttt acgtgtgaag ggctctccaa ggcgcaacgc agcgactgcg gccttctgcg
     6301 agatcgagca gggatttgag gtgctctgac tctggatatt ggccatcgcc gtcaccagat
     6361 cttttggacc ggccgcatat ccgattcgcc atcctgtcat ggcatacgac tttgatacgc
     6421 cgttgataat gactgttcgt gcagcgatct caggtcccaa cgaggcgatg ctgatgtgcc
     6481 tggctccgtc gtagagcacc ttttcataaa tctcatccga aatgacaagg agatcgtgcc
     6541 gaaccgccgc tgaagcaatc tgttccagcg tcgcccgatc ataggttgtg ccggtaggat
     6601 tgcacgggct gttcacgata atggctttgg ttctcggcgt aatgaggcgt tccaactctg
     6661 acgcatggac tgcataaccg tcttcctcgc tggtcggtag cagaaccggc gtcgcatcgt
     6721 tgagaagcgt ctgatccgaa taggaaaccc agtaggggat cggtatgata atctcgtcac
     6781 ccgcttcgag taaggcttcc gccaaattgt agagcgagtg ttttgccccg caggagacga
     6841 ggatttgaga cttctcatac cgtagcccaa gttcagcgtg aagcttgtcg gcgattgcat
     6901 tccgcaactc atcgatcccg gacgacggcg tgtatttggt aaaaccctcg cgtatggctg
     6961 cttctgccgc ggccttcacc ggatccgggg tgtcaaaatc cggttctccg gctgaaaagt
     7021 cgatgacgtc caatccttgc gcggccatgg ccttggcggt tgcagccata gccagcgttg
     7081 gagagggtgc aatacgaccg acacgtgccg ccaatttcat gatttgagac tccggatacg
     7141 agcttgtaga cgtcgggcta cttccggatg aagaaagtca gttacacttc caccgtgttg
     7201 tgccacgtct ttgataatcg tcgaggataa gtaggagtac tcttcactgg gcatcaagaa
     7261 cacggtctcc accatcttcg caagttttcg gttgacgagc gccatctgga attcatgttc
     7321 aaagtcggaa atggcacgca agccgcggat gattgcatgg gctcccgaac gctcgacata
     7381 atcgacgagt aatccgtcga acggtgtgac ctcgacctgt cctacatcct tcatcaccag
     7441 cgttatcatg tcaaggcgtt ccgccaaatt gaagagtggg tgtttagaag gatttggagc
     7501 gacggcgacc accaccttgt cgaacacacg gaggctgcgg gtgatgatgt cggcatggcc
     7561 atatgtaatc gggtcaaaag tgccggggta aataccgatt ttcatgccaa cacggcgtgg
     7621 gaacgggagt agagggacaa gaccgtatca ccatagcgat agcggcgcaa aaatttggtg
     7681 catcccagcg acatgggtaa ggtggtcttc acagcatgtt cgacgatcag ccatgagtcc
     7741 agggcaaacc actcatcggc ccccggcatg gcgagtattg atggaagttc cgacccctcg
     7801 gcataaggcg ggtcggcaaa gacaatgtca tagggaccgt tccactgatc gggatggtcc
     7861 agaaattgtt ggacagtctg atctagcacg gctatttcct caccgatatg acagagctcc
     7921 aggttt
//
LOCUS       DATNDD010000316         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_462334, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000316 DATNDD010000000
VERSION     DATNDD010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462334"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VN647_07635"
     CDS             <1..600
                     /locus_tag="VN647_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWG96940.1"
                     /translation="PLPDPENARDRVLTEEEWSKLHQVAKPHLKPVLQLAYQLGQRFG
                     EIVGLTWDRVDLKRGFITLRSQDTKTKKPRQIPMTPDVRMTLLRLAKVRSLSVRHVFT
                     FKGKPLQRISRSFRTALKDAGITNFRFHDLRHCASTNLRRAGVDTATAMKIVGHQSEK
                     SWKRYNAIEERDLLQAALKVQKYLQKNTPGTLDPKTEIL"
     gene            628..704
                     /locus_tag="VN647_07640"
     tRNA            628..704
                     /locus_tag="VN647_07640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:662..664,aa:Arg,seq:cct)
     gene            complement(763..984)
                     /locus_tag="VN647_07645"
     CDS             complement(763..984)
                     /locus_tag="VN647_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96941.1"
                     /translation="MLVFTVFAGVLMIMTPRIGSMVVSMHMLVLMFVVVGLSRSVHVF
                     MAVEVLMRVRTFHGWYSFQTIFSHDITLF"
     gene            1937..>2455
                     /locus_tag="VN647_07650"
     CDS             1937..>2455
                     /locus_tag="VN647_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96942.1"
                     /translation="MARARASEHQRPRSKTALIEEITHGINKFQEVLAAINDISQEGF
                     PYRDAAQSKAELQFRECLRQTFGERSHEFHTHRNFKIRTADKAEVAQTLTVIKGLIQI
                     LEDRKLDHQGLKPPPKPEPPHEATINNTARLMLVSPMTPTTVTAQAATPMTVAMAMTT
                     NVGPSIPFVTPTP"
BASE COUNT          635 a          701 c          568 g          551 t
ORIGIN      
        1 ccgcttcctg atccagagaa cgcaagagat agagtattga ctgaagagga atggagtaaa
       61 ctccatcaag tggcaaaacc gcatctgaag ccggtcttac aactcgccta tcagctaggc
      121 cagaggttcg gtgaaattgt cgggcttacc tgggaccgtg ttgacctcaa gagaggcttt
      181 atcactctcc gcagtcaaga cacgaagact aagaagccaa gacaaattcc catgacgcca
      241 gatgttcgga tgacactcct acgactggca aaagttcgat cgctttcggt acggcatgta
      301 ttcacgttca aaggaaagcc tctgcaacgt atcagtcggt ctttccgaac cgctctaaaa
      361 gatgccggta tcaccaattt caggttccac gatcttaggc attgcgcatc taccaatctc
      421 aggcgggcgg gagtagacac cgccacggct atgaagattg tcggccacca gtcggagaag
      481 agttggaaac ggtacaatgc aatcgaggaa cgcgacttgc ttcaagcagc tttgaaggtt
      541 cagaagtacc tccagaagaa cacgccggga acacttgacc ctaagaccga gattctgtag
      601 aactgtaagt cattgaaatt tggccttgcc cccgtagctc agttggatag agcagcggtt
      661 tcctaaaccg cgggtcgtac gttcaattcg tatcgggggc accatccttc tcctcttatc
      721 cgacttgcgt ccgataagcc gcgtattgcc gtattccatg actcaaaaaa gagtgatatc
      781 atgggagaaa atcgtctgaa aggagtacca cccatgaaag gttctcactc tcataagcac
      841 ctccacagcc atgaacacat gcacggatcg gctcaaaccc acaaccacga acatgagcac
      901 gagcatgtgc atgctcacga ccatggatcc gatacggggc gtcatgatca tcagcacgcc
      961 ggcgaacacg gtgaacacca gcacgagcac tcaatgtagt tcccttgtgc acgtacatcc
     1021 gcgccgatca cagctttctt ctaatgctgt cgttagcaca ttctatgttg aatgtgcatt
     1081 gcccgttcgt tgttccgctg gttcgagtaa tcgaaccacc acctatcgtc aacacctcca
     1141 ttcatctctc gatccatgca acggctctta ggattttata ctcttctttt tctctcgctt
     1201 tctactatag attcttagct atagcatcct aagcatttta atcctttttc tgttacccgt
     1261 tccacacgat cggcgaggac cattacctat gaaaggggga cttatgccgg acgcaagcag
     1321 aagaattgtg accaccgatg cttctttgct cgcgacggat gcttcgctga tgatactgcg
     1381 aggagtaaat gccgcgacgc acgaggcgat gcgggaagat catcacatga gcgaagagac
     1441 gaggccctgt atacagttct gtcaagactg tcatgcatca gacgaagagg ggcaaccagt
     1501 tcgtgagttg acatgtgctt gcaacaatgc atcacagaac aatgctcaac gaaagacttg
     1561 cacgcttcat ggtgacctcc catttccacg gactcccccg ttttacagga gacggagacc
     1621 tgtctggatt ggataattcg actatctggc gtacaaaact atctgggcaa tacctattcc
     1681 tctgcctgga cgccgccaca gacatgccgt cgttccatcc ccgatcaatc cgtactcttc
     1741 tcacggcaga tatccgtggc ggcagacgag ggggtttcca tccgttgcta tctcgatctc
     1801 ccgtcgtcat ctaaactcgg tttccggtaa gccttgctcg cagcctccag cccctgatat
     1861 cacgggcata ccccgtcaaa atttgctgtt ttcttgtcat gcggtacccc ctcagttaga
     1921 cttttttcac acaatcatgg cacgcgcacg cgcatcggaa catcagcggc cccgatcgaa
     1981 aaccgctctc atcgaagaga tcacgcacgg aatcaacaag ttccaggaag ttctggcggc
     2041 aatcaatgac atcagccaag agggattccc ttatcgtgat gccgcacaaa gcaaagccga
     2101 actgcaattt cgtgaatgcc tgcggcagac gttcggtgag cgttctcacg aattccacac
     2161 acatcggaat ttcaagattc gcacggcgga caaggccgag gtggcacaga cccttaccgt
     2221 cattaaaggt cttatccaaa tcctggagga ccggaaactg gaccatcaag gactcaaacc
     2281 gccacccaaa cccgagcccc cgcatgaggc aaccataaac aacaccgcac gtctgatgct
     2341 tgtctcgccg atgacaccaa ccaccgtgac agcacaagcg gctactccga tgacggtcgc
     2401 catggctatg acgacgaatg tgggaccgtc aatccccttc gtcacaccca cgcct
//
LOCUS       DATNDD010000317         4479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2936258, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000317 DATNDD010000000
VERSION     DATNDD010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2936258"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VN647_07655"
     CDS             <1..616
                     /locus_tag="VN647_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWG96943.1"
                     /translation="EELYSDLPRLEQFMGAMIGASRINFEAFAEKFDFSKLQTLCDVG
                     GATGLLSIEVAKRHPHLTCTTFDLPVVEPIAKKYIAAAGLTSRVRTSSGDFFKDPLPN
                     ADIITMGMILHDWNLEKKMHLIRSAYKALPPGGALVAIEALIDDARRENVQGLLMSLN
                     MLIEFGDAFDYSGADFRRWCGEVGFTRFEVIHLAGPSSAAVAYK"
     gene            705..1415
                     /locus_tag="VN647_07660"
     CDS             705..1415
                     /locus_tag="VN647_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG96944.1"
                     /translation="MGASNHKKILIVEDEQDILQLVKHYLEKEGFRAITAMNGLDALK
                     KVKEEKPALVVLDLMLPEIDGLEVCKRLRSVPDTAMLPILMLTAKAEESDTIVGLELG
                     ADDYVTKPFSPKTLIARVKALLRRIERVPADGPNLYHYGALTMSLDRHEVSLGKQEVP
                     LTAKEFGLLEHLLRHRGRVLTREVLLNAVWGYDYYGTTRTVDVHIRRLKQKLPLLEEA
                     IVSVKSLGYKLKDFDVSE"
     gene            1412..3271
                     /locus_tag="VN647_07665"
     CDS             1412..3271
                     /locus_tag="VN647_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG96945.1"
                     /translation="MTWSIRWKVAFGTLVAVACGLLIAGVMTVQFLEQRYLTQFGDGL
                     EVKTKLVEYGFHPLFQSSSSPPTPFSLQETARDLGNRASARVTLVAADGTVLADSAVR
                     DVDLSAVENHKSRPEIQQALSTGQGRDIRSSHTTGERTMYRAVLMQPSQIASPLVVRV
                     GLPMVMLDHKLSEFQQRLFLALGVAFLVALTLSVWLAHSITKPLSDIASAARQFSSGN
                     QTFHVRTTAQDEVGLLASTLNHMADQLHTKIDELSEDRAQLLAVLTSMVEGVMVLDSR
                     GHVLQINPALERMFGISRVEARGRPCAELFRHQQLNDLVTAILRSRTHHQDEIVLPLS
                     GRCLQIEASPAGGERENEASVVLVLHDITELRRLETIRKDFVANVSHELRTPLTSIKG
                     YVEALLDGAKDNPAASTKFLEIILKQSDRLNLIIEDLLELSKIESGGISLKEEPLELR
                     SVIDRTLSMIKPIADKNRHRLVTTVDPALPPVAGDEDRLVQVLTNLLDNAIKYTPAGG
                     TITVGAKLTPSVGSTDPPARAIELSVADTGIGIPEEDRPRVFERFYRVDKARSRELGG
                     TGLGLAIVKHIVEGHRGQVWVEANHPQGSRFVVRLPLSTTARSLHQVNEASRA"
     gene            complement(3244..4236)
                     /locus_tag="VN647_07670"
     CDS             complement(3244..4236)
                     /locus_tag="VN647_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWG96946.1"
                     /translation="MTELSGILLLTVVLALLFDFSNGWHDCANAVATVVSTRVLSPIA
                     AVLLAGVLNVAGAFFSTAVAKMIGGGIVFSDAITNTVVAGAMGGAILWNFLTLMLGLP
                     TSSSHALIGGLVGSAVAHAGWAVVQFNGLRKILEAMILSPLLGFGIGFLLMVLVSWVF
                     FRVHRGAATKIFSRLQIVSASFMAFSHGANDAQKVMGVITLALVASGQLTSSEVPTWV
                     IVACALAMGLGTTVGGWRIIRTLGIKMVKLEPVHGFCAETAAASVLLFAAHFGLPVST
                     THTITSSILGVGSTKRLSAVRWGVTNKIFSAWVFTLPGAALLGAGVYALLASFT"
BASE COUNT         1014 a         1310 c         1213 g          942 t
ORIGIN      
        1 tgaggagctc tattcggatc tccccaggtt ggaacaattt atgggtgcga tgatcggtgc
       61 ctcacgcatc aatttcgagg cgttcgcgga gaagtttgat ttctcgaaat tacagactct
      121 ctgcgacgtc ggcggcgcaa ccggtctcct cagcatcgag gtcgcaaagc gacacccgca
      181 tctcacatgc accaccttcg atctgccggt cgtcgagccg atcgctaaaa aatacattgc
      241 cgctgccggc ttgacaagtc gtgtgcgcac gtcgtccggc gacttcttca aagaccctct
      301 gccgaacgcc gacatcatca cgatgggcat gatcctgcat gactggaatc tggagaaaaa
      361 gatgcacctg atccgttcgg cctacaaggc gttgccgccg ggcggtgcgt tggtagccat
      421 cgaagcgttg atcgatgatg cccgccgcga gaacgtgcag ggactgctga tgtcactcaa
      481 catgctcatt gaattcggcg atgcattcga ttattccggg gctgattttc gccggtggtg
      541 tggcgaggtc ggattcacac ggttcgaggt cattcacctg gctgggccgt cgagcgcggc
      601 agtggcctac aaataaccgc ccatgaatga gcgggccgcg gtcttgatgg cttaccaccc
      661 attctcttga ctctctctcc cctcccccgt acgctcactg ttccatgggg gcttccaatc
      721 acaagaaaat cctcatcgtt gaagacgagc aggacattct gcaactcgtc aagcactatt
      781 tggagaaaga ggggtttcga gctatcaccg ccatgaacgg gcttgatgct ctgaagaaag
      841 tcaaagagga gaaacccgct ctggtcgtcc tcgatctaat gttacccgag atagacggac
      901 tcgaagtctg caaacgcctc cgctccgttc ccgataccgc tatgctcccg atcctcatgc
      961 tgacggcgaa agccgaagag tcggacacga tcgtcggtct tgaattgggc gccgatgatt
     1021 atgtgaccaa accgttcagc cccaaaaccc tgatcgcccg cgtgaaggcc ctgctgcgtc
     1081 gaatcgaacg cgtgcctgcc gacggcccaa atctatatca ctatggtgcg ctcaccatga
     1141 gtctggatcg gcatgaggtc agtctcggga aacaagaggt gccgctgacg gcgaaggaat
     1201 ttggcctgct cgagcacctg cttcggcatc gcggtcgtgt attgacccgg gaagttctgc
     1261 tcaatgccgt gtggggctac gattactatg gcacgaccag aacggtcgac gtgcatatcc
     1321 gccggttgaa acagaaactg ccgcttctcg aagaagccat cgtgtccgtg aaatcattgg
     1381 gatataaact gaaagatttt gacgtgtcag aatgacatgg tcgattcgat ggaaagtggc
     1441 attcggtacg ctcgtggcag tcgcctgcgg gctgctgatt gccggcgtga tgacggttca
     1501 attcctcgaa cagcgatacc tcacgcaatt cggtgatggg ctggaggtaa aaaccaagct
     1561 cgtcgaatac ggctttcacc cgctgtttca atcatcttct tcgcctccga cccctttctc
     1621 cttgcaagaa acggcgcgcg acctcggcaa tcgagcctcc gctcgtgtga cgctggtcgc
     1681 cgcagatgga accgtccttg cggatagcgc cgtgcgtgat gtcgacttgt ccgccgtcga
     1741 gaatcacaag tctcgacccg aaatccaaca ggccctttcc acaggacagg gacgggacat
     1801 ccgctccagt cacacgaccg gtgaacggac catgtatcgc gccgttctca tgcagccatc
     1861 acagattgcc tcgccgcttg tggtgcgggt aggtcttcca atggtcatgc tggaccataa
     1921 actctcagaa ttccagcaac gtctctttct cgcgctcggg gttgccttcc tcgtcgccct
     1981 gacgctgagc gtctggctgg ctcacagcat taccaaacct ctgtcggata tcgcgtcggc
     2041 cgcccgccaa ttcagttccg gtaaccagac atttcatgtc agaacgaccg cgcaagatga
     2101 agtcggcctc ctcgcttcga cgctcaatca catggcggat cagcttcata ccaagattga
     2161 tgaactctcg gaagaccgtg ctcaactgct tgccgtactg acctcgatgg tcgaaggcgt
     2221 gatggtgttg gactctcgcg gccacgtatt gcagatcaat cccgctttgg aacgcatgtt
     2281 cggcatctcc cgtgtggagg cacggggaag accctgtgcc gaactctttc gtcatcaaca
     2341 gctcaacgat ctggtcacgg ccattttacg ttcacgtacc catcatcagg atgaaatcgt
     2401 gctgccgctg agcgggcggt gtcttcagat tgaagcctcc cctgcaggag gtgaacggga
     2461 aaacgaagcc agtgtcgtgt tggtgcttca cgatattacc gaattgcggc gcctggaaac
     2521 gatacggaaa gatttcgtgg cgaatgtctc tcacgaactt cgaaccccgc ttacctcgat
     2581 caagggctac gtcgaagcgc tgcttgatgg ggccaaggac aatcccgccg cttctacgaa
     2641 attcttggaa atcattctca agcaaagcga tcggctgaat ctaatcattg aagatttgct
     2701 cgagctgtcc aaaatcgaat cgggcgggat ctcgttaaaa gaagaaccgc ttgaactgcg
     2761 ttccgtcatt gaccggacgt tgtcaatgat caagccgatc gccgataaaa atcgtcaccg
     2821 gctcgtcaca acggtcgatc cagcactccc tccggtagcg ggtgatgaag accggctcgt
     2881 gcaagtcttg accaatctgc tcgacaacgc catcaaatat acgccagcag gagggaccat
     2941 tacggtgggc gccaagctga ctccttcggt cggaagtact gatccaccgg ccagagcgat
     3001 cgagctcagc gtcgccgata ctggaatcgg tatccccgag gaggaccgac ctcgtgtctt
     3061 tgaacggttt taccgcgtcg ataaagcccg gtcacgagag ttgggaggga cgggactcgg
     3121 cctggcgatc gtgaagcata tcgttgaagg gcatcgagga caggtatggg tggaagcgaa
     3181 tcatcctcaa gggagtcgat tcgtggtccg tcttccgctc agcaccactg cccgcagcct
     3241 ccatcaggtg aacgaggcaa gcagggcata gacacccgct cccaacagcg cggctccggg
     3301 cagtgtaaat acccaggctg aaaagatttt gttcgtcact ccccatcgaa ccgcagacag
     3361 acgcttggtc gatcccacgc ctaagatcga ggaggtgatg gtgtgggtcg tgctgaccgg
     3421 aagaccgaag tgcgcggcga acagcaacac cgacgccgct gccgtctctg cgcagaatcc
     3481 atgaaccggc tcaagtttca ccattttgat ccctagagtc cgaatgatcc gccacccgcc
     3541 gacggtggtt cccagcccca tcgccaatgc gcaggccacg atcacccaag tcggcacttc
     3601 cgatgatgtc aattgaccgg acgccacgag ggcaagcgta atgacgccca tcactttttg
     3661 cgcgtcgttc gccccatggc tgaatgccat gaagctggcc gaaacgattt ggagtcggct
     3721 gaatatcttt gtcgcggcac cccgatgaac ccgaaaaaag acccagctga ccaggaccat
     3781 caggagaaat cctatcccga accccaagag gggggaaaga atcatggctt cgagaatctt
     3841 gcgcagaccg ttgaactgaa ccactgccca gccggcgtgc gccaccgctg agccgacgag
     3901 tccaccgatc agcgcgtgcg acgaactggt cggcaggccc agcatcagcg tgagaaaatt
     3961 ccagagaatc gcaccgccca tcgctccggc gaccaccgta ttggtgatcg cgtcggagaa
     4021 gacgatgcct ccaccgatca ttttcgccac ggccgtcgaa aagaaggctc cggcaacatt
     4081 taacaccccg gccaacagca ctgcggcgat ggggcttagt acacgggtgg agacgaccgt
     4141 cgccacggca ttggcgcaat catgccaccc attcgaaaag tcgaacagga gcgccaacac
     4201 caccgtcaga agtagtatgc cgctgagttc agtcatagtc cggttgaaat acttcgccta
     4261 tccgaggatc attcgcgcat ggagggtcga ggacacacag atttcatggc caggcattct
     4321 attcgcgagc gactaggtct gcttgagaac aatgcactcg atcacgttgg cgacatcctc
     4381 acaacgatcg gttccttctt cgaaggtttc ataaatctct ttccacttga tgacggcgat
     4441 cggattcgtc tctttctcga acaactccga aatggcatc
//
LOCUS       DATNDD010000318         5384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1750611, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000318 DATNDD010000000
VERSION     DATNDD010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750611"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VN647_07675"
     CDS             complement(<1..1447)
                     /locus_tag="VN647_07675"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWG96947.1"
                     /translation="MGEYALLYILFAPFAGALGLIFISNRQPLLVRSIAASAAFVSLI
                     SSFYLFYAYDPVKGGFQFVQKFEWCRQLGISLYLGVDGIGTPLVLASSILLFAGIFVS
                     WHIKDRTKEFYIWLLILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDPEGLKLRDSVGFILNFGSNSKEYAAMKLVLFLSAFAVVALMGILLIYKYSGLN
                     TFDILVLREQAKLMNIPVLGTTLDKIIWVLVFFGFASIAPLWPLHSWSPVGHAAAPAA
                     TSMLHAGVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCMFSIMYGGFVAFYAKDTK
                     YVIGYSSSSHMGYVFLGMAALNYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHSRD
                     IPSLGGLSNKMPFISACFIVACMASIGMPGTVNFIAEIMIIVGSWNKYPVQVIVAMLG
                     IVLTLAYLFKMMRGLFYGPMSQKYSHAHDAIS"
     gene            complement(1456..3015)
                     /locus_tag="VN647_07680"
     CDS             complement(1456..3015)
                     /locus_tag="VN647_07680"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWG96948.1"
                     /translation="MLEELTAGFPILSCILFFPVVGAVVLWLFDEEDMVRTSALTIAL
                     VELALSVFVLLRFVPESAAMQFTERVRWIPALGISYHLAVDGISVLFVGLTAFLTVLV
                     VIYSWDTIRHQMKLYMMCLLALETTTMGVFVSLDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKFVIYTLLGSVFMLVGIALLNINYHQWASLHHTDQVYSFDLLELLTVPI
                     PISQQILIFWLMFMGFAFKAPVFPFHTWLPDALLEGPIGMAVVLAGLKLGTFGFIRFS
                     IPLLPDASKSETVVMVVVVLGLCAILYGAWMALVQADFRRLLAYSSVSHLGFVIVGLF
                     ALNYQGLQGSLLTMINLGFSTAGLFFIAGFLYSRQQTTQLSAFGGMAKQVPLLATFFL
                     IIGLASIGLPGTNGFVGEFLILLGAFKAKWWFGAIAVLGVIFGAAYFLWYYERSMLGP
                     LGAAVKGTMSDLQPREKIIALSLSIMILWIGLYPSPFLRIMNGSVQALVDRVSQETKV
                     SLEGARFGREY"
     gene            complement(3041..5017)
                     /gene="nuoL"
                     /locus_tag="VN647_07685"
     CDS             complement(3041..5017)
                     /gene="nuoL"
                     /locus_tag="VN647_07685"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWG96949.1"
                     /translation="MSDLTDLLIKLIPAFPLLAVLVNGLVGHRYSHDLAHRLAWGSVG
                     LSFLCTVGVFTDVMRTGASHEVIAYQWIFGGDLTINLAYLVDPLTCAWLLIVTGVGFL
                     IHVYSVGYMYGETGFTRFFTYMNLFMVSMLLLVMGNNYVVLFIGWEGVGLCSYLLIGY
                     YYDKVSAAKAASKAFVVNRIGDAGFLVAIFLVFINFRTLDYTKIFPQVGQLSPDMATA
                     IALCLLVGAIGKSAQLPLHTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDL
                     SPFAMSMVAFVGGGTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGIGAYTASVF
                     HVMTHAFFKALLFLSAGSVIHALSGEQDIRKMGGLSSRIPWIHRLFLIGTIAIAGLPP
                     LAGFWSKDEIMAHAFTSQHYLFYGMAALGALMTSFYMFRLTYLTFYGSSRMDPHTAEH
                     VHESPMVMVVPLMVLALLSIVGGLILGFPPEHGWLHGFLGSVVGVGGEHETSAGMVFL
                     LMSIAILIALMGWGLAHFLYAVSPATADGWAEKFSGLYRVLWNKYYVDELYDLIFVEP
                     LKRLGAMLDWFDRTVIDGIVRGIGRLADWSSMGSTWIEKYIIYGGLNVIGYGNHLAAR
                     EGRKMQSGMVHHYAAIIVAGLFLLVLVVQLFAQM"
     gene            complement(5154..>5384)
                     /gene="nuoK"
                     /locus_tag="VN647_07690"
     CDS             complement(5154..>5384)
                     /gene="nuoK"
                     /locus_tag="VN647_07690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWG96950.1"
                     /translation="RRNFIIVLMSVEIMMNAANINLVAFSHYLESMAGQLVALFIIAI
                     AAGEAAVGLAIIIVVFRGKISTNVDEMNLLKW"
BASE COUNT         1555 a         1468 c         1362 g          999 t
ORIGIN      
        1 tagaaatcgc atcgtgcgca tgactgtact tttgactcat tggaccgtaa aaaagcccgc
       61 gcatcatttt gaagagatac gccaaagtca gaacgatgcc taacatggcg acaattactt
      121 gaaccgggta cttgttccaa cttccgacga tgatcatgat ttccgcgata aaatttactg
      181 tgccgggcat cccgatcgaa gccatacagg ccacgatgaa acaagcggag atgaacggca
      241 tcttgttcga gaggccgccc agcgaaggaa tatcacgtga atgggtttgg tcatagaccc
      301 atcctgccat ggcgaagagc ataccggtcg ccatggcatg ggcaaacata tagatgacgg
      361 cgccgctcag gctgatgtaa ttcaaggccg ccatgccgag gaacacataa cccatgtgac
      421 tggaacttga gtacccgatt acgtacttgg tgtctttggc gtaaaacgcg acgaagccgc
      481 cgtacataat gctgaacatg cacagaacag ccgcaatcgg catcagttcc ctggttgttt
      541 cagggagaat ctcgaaggcc acgcgaataa tggaaaagtg cccgagcttc atgaggacgc
      601 ccgcatgaag catactggtc gcagccggcg ctgccgcatg gccgaccgga gaccaggaat
      661 gcagtggcca cagcggtgca atcgacgcaa agccgaagaa caccaacacc cagatgattt
      721 tgtccaatgt cgtgccaagc accggaatat tcatcaactt ggcctgctca cggagcacca
      781 ggatatcgaa ggtgttcagg ccggagtatt tatagatgag taaaatgccc atgagcgcga
      841 cgacggcaaa tgcggacagg aagagcacca gtttcatcgc cgcatattcc ttgctgttcg
      901 acccaaaatt gaggatgaat ccgactgagt cccgcagttt cagaccttcg ggatcggtca
      961 tctccaaata cttcttggtg tggctgcccc acatccccaa cagcaaatac atcggaatca
     1021 cggacatctc atagaagaag tagaggaaga acaggtcgag ggacatgaac acgccgatcg
     1081 tagcggcagc caagatcagc agccagatat aaaactcttt ggtccgatcc ttgatatgcc
     1141 aagagacgaa gatgccggca aacaacaaga tggaggaggc gagcacgaga ggagtcccga
     1201 tcccatctac gccaagatac aatgaaatgc cgagctgcct gcaccactca aacttttgga
     1261 caaactggaa cccgcccttc actggatcat aggcgtaaaa gagatagaac gacgagatca
     1321 gcgacacaaa tgcggcactt gctgcgatac ttcgaacgag cagaggctgt cgattggaaa
     1381 taaaaatcaa ccccagagcc cccgcaaacg gggcaaacag gatatagagc agtgcgtact
     1441 ctcccatcgc gcgacctaat actctctgcc gaatcgggcg ccttctaatg acaccttggt
     1501 ctcctgagag actcgatcaa cgagcgcttg gaccgatccg ttcatgattc gcaagaacgg
     1561 tgagggatag agcccgatcc agaggatcat gattgacagc gacagcgcaa tgatcttctc
     1621 gcgcggttgc agatcgctca tcgtcccttt cacggcagca cccaacggac ctaacatcga
     1681 acgttcgtag taccagagaa aatacgcggc gccgaaaatc actcccagaa cggcgatggc
     1741 cccgaaccac catttggctt tgaacgcgcc caaaagaatg aggaactcgc cgacaaagcc
     1801 attggtaccg ggaagtccta tcgacgcgag tccgataatc agaaaaaagg tcgctagaag
     1861 aggcacttgt ttcgccatcc ctccgaacgc cgataactga gtcgtctgct ggcgcgagta
     1921 aagaaacccc gcgataaaga agagtcctgc cgtgctgaac cccagattga tcatcgtgag
     1981 caagctgcct tgcaaacctt gatagttcaa tgcaaacaac cctacgatga cgaaaccaag
     2041 atggctgaca ctgctatagg ccagcaatcg tcgaaaatcg gcttgaacca aagccatcca
     2101 agccccatag agaattgcgc agagcccgag cacgaccacc accatgacaa ccgtctcgct
     2161 tttcgacgca tcggggagca ggggaatgct gaagcgtatg aagccgaatg taccgagctt
     2221 gagaccagcc aacaccacag ccatcccgat cggcccttcc agcagggcat cgggcaacca
     2281 cgtatggaat gggaacaccg gcgccttgaa cgcaaatccc atgaacatca gccagaagat
     2341 aaggatttgt tgactgatgg gaatcggcac cgtcagcagt tctagcaaat cgaacgaata
     2401 gacctgatca gtgtgatgaa gggatgccca ctgatggtaa ttgatgttca gcaaggcgat
     2461 gccaaccagc atgaacacac tgcctaaaag cgtataaatg acgaacttca gcgcggcata
     2521 gtgacgctcg gctccccccc cccacaactt gatcaagaag tagctgggaa tcagcatcag
     2581 ctcccagaac acgaagaaca gtatcaaatc cagagacacg aagacgccca tcgtcgtcgt
     2641 ctcgagcgcc aatagacaca tcatgtagag tttcatttga tgtcggatcg tatcccagga
     2701 gtagatcacc accaagaccg tcagaaaagc cgtgagcccg acgaacaaca cgctgattcc
     2761 atcaactgcc aggtgatagc tgatgcctaa cgccggaatc cagcggacac gctctgtgaa
     2821 ttgcatggcg gccgattccg gcacgaaacg cagcaggaca aacacagaaa gtgccagctc
     2881 aaccagcgca atggtgagcg ctgaggtccg gaccatatct tcttcgtcga acaaccacag
     2941 cacaaccgct ccgacgaccg ggaaaaatag aatgcaggac aaaatcggga atccggctgt
     3001 gagctcttca agcatggacg cagtctcgtc tggttaagac ctacatttga gcaaacaatt
     3061 gaacgacaag aacgagtagg aagagtcccg caacgatgat ggcggcataa tgatgaacca
     3121 ttccactttg cattttccga ccttcgcgag ccgcgaggtg gttaccgtat ccgatcacat
     3181 tcaggccccc gtagataata tacttctcga tccacgtgct tcccatggaa ctccaatcag
     3241 cgagtcgtcc aataccccgt acgataccgt caatgaccgt gcgatcaaac cagtcaagca
     3301 ttgcacccag acgtttgagg ggttctacga agatcaggtc gtacagttcg tcaacatagt
     3361 atttgttcca gagaactcga tacagaccgg aaaatttctc agcccatcca tcagcagtgg
     3421 ccggactcac ggcatagagg aaatgtgcga gtccccatcc catcaaggca atgaggatcg
     3481 caatgctcat caacagaaac accatgccgg cactggtctc atgctcgccc ccaaccccaa
     3541 cgacggatcc caaaaatcca tgcagccagc catgctccgg gggaaatccc aatatcaatc
     3601 cacccacgat cgataacaag gcaaggacca ttaaggggac gaccatgacc atcggagatt
     3661 cgtgcacatg ctcagccgta tgaggatcca tgcgagaaga accgtagaac gtcagatagg
     3721 tcaaacgaaa catataaaaa gacgtcatca gcgcccctaa ggccgccatg ccatagaaga
     3781 gatagtgctg actggtgaag gcatgggcca taatttcatc tttgctccag aagcccgcga
     3841 ggggaggaag cccggcgatt gcaatggtcc cgatcagaaa taggcgatgg atccaaggaa
     3901 ttcggctgct cagtccaccc atttttcgga tatcctgttc gcccgataat gcatggatca
     3961 ccgaccctgc cgacaagaac aacagtgcct tgaagaacgc atgcgtcatg acatgaaaaa
     4021 cggacgccgt ataggctccg atcccgcaac cgagaaacat atatccgagc tggcttacgg
     4081 tcgaataggc caagacacgt ttgatgtcgg tctggacgag tccgatggtc gccgcgaata
     4141 atgcggtccc tccaccgacg aatgccacca tcgacatcgc gaacggtgaa agatcaaaga
     4201 tcgcgtgatt acggacgatc atgtagacac cggcggtcac catcgtcgcc gcatggatca
     4261 atgcgctcac cggtgttgga ccttccatcg catcgggcag ccaggtgtgg aggggtagct
     4321 gtgcggactt cccgatcgcc ccaacgagga ggcagagagc gatggcggtg gccatgtccg
     4381 gagacagttg cccgacctga ggaaagatct ttgtatagtc gagagtccta aagttgataa
     4441 agacaagaaa tatggcgacg agaaatcccg cgtcgccgat ccggttgacg acgaatgctt
     4501 tggatgctgc cttggcggcg gaaaccttgt catagtaata accgatcaag agatacgaac
     4561 aaagcccgac accctcccaa ccgataaaga gcacgacgta gttgttcccc atgacaagaa
     4621 gcaacatgga gaccatgaat agattcatgt aggtgaagaa gcgcgtaaat ccggtttctc
     4681 cgtacatata gccgacggaa tagacgtgaa tgagaaaccc gacaccggta acgatcagca
     4741 accaggcaca ggtcagagga tccaccaggt aggctaaatt gatcgtgaga tcccccccga
     4801 agatccactg ataggcaatg acttcatggg aagctcctgt acgcatgaca tcggtgaata
     4861 cgccgaccgt acagagaaac gatagaccga ccgaacccca agccaatcga tgggccaggt
     4921 catgagagta gcggtggccg accagtccgt tcacgaggac cgcgagcagc gggaaagcag
     4981 gaatcaattt gatgaggaga tcagtcaaat cagacacagt atcgtgccat tctaaggatg
     5041 ctcagctaac caaccatgct gcatggagcc ggaacctaaa gcgtactctt gcgggtacgt
     5101 tgagggggta cgaaccatcg actgcaccgt tggaggtgat tgtccacatc ctactaccac
     5161 tttaataggt tcatttcatc cacattcgtg gaaatcttgc ctctgaacac gacgatgatg
     5221 atggccaaac cgacggccgc ttcgcccgcc gcaatggcaa tgatgaacag agccacaagt
     5281 tgccccgcca tagattccaa ataatgtgaa aaggcgacta aattgatgtt ggccgcattc
     5341 atcatgattt caaccgacat cagcacaatg atgaagttcc gcct
//
LOCUS       DATNDD010000319         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3586351, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000319 DATNDD010000000
VERSION     DATNDD010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3586351"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..708
                     /locus_tag="VN647_07695"
     CDS             325..708
                     /locus_tag="VN647_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96951.1"
                     /translation="MSGERTCPTCNTEKAQQISDILRQTPSKETLGQVPSSYHPPKAP
                     WAYLQGFLLGIPVNAAVMLSLASPQGTEAEMAMADLASSIAFLGVWVGYGALKTKNYT
                     QKLNEWKDTIASKFLCRKCSHAFEG"
     gene            complement(806..1213)
                     /locus_tag="VN647_07700"
     CDS             complement(806..1213)
                     /locus_tag="VN647_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96952.1"
                     /translation="MIESIGTSLSESQRGFLFGSILTFVSTFAWLVLAGSFGISEWTE
                     YFVIVGILAQVLLIHRREQALYTSRRWRFWYTLIIAGFACDLMTRHLWQQGHDSITDP
                     TQVRGQLMLGGLLLFYVMTFPITLRLILRKTKS"
     gene            complement(1367..1444)
                     /locus_tag="VN647_07705"
     tRNA            complement(1367..1444)
                     /locus_tag="VN647_07705"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1406..1408),aa:Glu,seq:ttc)
     gene            complement(1566..2126)
                     /gene="rfbC"
                     /locus_tag="VN647_07710"
     CDS             complement(1566..2126)
                     /gene="rfbC"
                     /locus_tag="VN647_07710"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWG96953.1"
                     /translation="MRVTSIDIPGVLLLEPSVLSDHRGCFMETYHEQRYADGGITEHF
                     VQDNCSRSVRNTVRGLHFQEPHAQGKLVMALEGTVYDVVVDIRKGSPTFGKWYGIELS
                     GKNLHQMYIPPGCAHGFCVTSETATFLYKCTAYYSPRDERGILWDDPALGIVWPISEP
                     ILSQKDRAYRTIKEMEAELPFYKPVH"
BASE COUNT          552 a          575 c          643 g          529 t
ORIGIN      
        1 ggggtggcgg cgcagatcgt gagccggatg tcgaatctgg cggacgtgat ttggaacaac
       61 gcgccgaaga gcatcctgga tccgttcccc agccaggtgg gtcccgggca taagaccgtc
      121 tactaaaggg tcctagtaga gtgtgagagt cggcccggaa ttcccgacaa ggggggttcc
      181 gggccgaaat gtttgtgagt caaaagttcc atagcgaaga gccttcctcc tcttgcacca
      241 aggaattctt cctgcctaga atagtcaggt acgtggtcaa acgacatgag ttctgagagc
      301 tgacacccag aaaggaggag tcatatgtct ggtgaacgca cttgtccgac ctgcaatacc
      361 gagaaggcac aacaaatcag cgatattctt cgtcaaacgc cgtccaagga gacattgggt
      421 caggtgccga gctcctatca tcctccaaag gccccttggg cctatcttca ggggtttttg
      481 ctgggtatac cggtgaatgc tgctgtgatg ttaagtctcg catctccaca ggggaccgaa
      541 gccgagatgg cgatggccga tttagcttcc tctatcgcct tcctaggggt atgggtggga
      601 tatggagccc tgaagacgaa gaactacact cagaagctaa atgaatggaa ggatacaatc
      661 gcgtcgaagt tcctgtgccg gaagtgcagt catgcgtttg aaggatagac ttctcgtggt
      721 agccgctttc tggatttctt ccgctggaat cagctttgcc tcactcgaga caggataagt
      781 gatactagct agtgtgggcg ctctctcatg actttgtttt tcgaaggatc aggcggaggg
      841 tgatcggaaa tgtcatgaca taaaataaca gcaggcctcc cagcataagc tgaccgcgaa
      901 cttgtgtggg atccgtgatt gagtcatggc cttgctgcca gagatgcctg gtcatgaggt
      961 cgcatgcaaa gcccgcgatg atcaacgtat accagaagcg ccagcgccgt gatgtataca
     1021 gtgcctgctc tcgtcggtgg atcagaagga cctgagcgag gatgcctaca atcacaaagt
     1081 attccgtcca ttcagaaatc ccgaaagagc cagccagtac aagccaggcg aaggtgctga
     1141 cgaaagtcaa aatgctccca aagaggaatc ctcgttgtga ttccgagagg cttgtgccga
     1201 tgctttctat catttatccc taccgggcac atttcgtgct ccgtacctgt ttggctagat
     1261 gtcgaccatg cgctggttca tgggttcgaa tatgggaatg ttcatgtttc ttcggcactg
     1321 tccccgaagc agttaacggt gtcggcggaa ggaccctgaa aagaactggt gtccccaacg
     1381 ggatttgaac ccgtgttact gccttgaaag ggcaatgtcc taggccaggc tagacgatgg
     1441 ggacgtgccg ctatgggtaa gagaattgca cacataccat attcgtaacc ggtctgtcac
     1501 taggattggc tccagggttc gggacactcc tttgcctcgc gataggaatg agagtggatg
     1561 aggggctaat gaacaggttt atagaacggc aactccgctt ccatttcttt gatggtgcgg
     1621 tacgctcggt ctttctgtga gagaataggc tcactgatcg gccaaacaat ccccaaagcc
     1681 ggatcgtccc acaagatgcc gcgttcatct ctcggcgaat aataggccgt gcacttatac
     1741 agaaacgtcg ccgtctcgct cgtgacacaa aaaccatgag cgcatcccgg gggaatgtac
     1801 atttggtgaa ggttcttacc cgacaactcg atcccatacc attttccgaa tgtcggcgac
     1861 ccctttcgga tatcgacgac gacgtcgtat accgtcccct caagggccat gacaagtttc
     1921 ccttgagcat gcggttcctg aaaatgcaga ccgcgtaccg tgtttcggac cgagcgggag
     1981 cagttatctt ggacgaagtg ctcagtaata cctccatcag cgtagcgctg ctcatgatat
     2041 gtttccatga agcaccctct atgatcagac aggacggacg gttcaagcaa gagtacgcca
     2101 ggaatgtcaa ttgacgtcac acgcacggca tcctctattc atggcttcgg atcggagtcg
     2161 cgagatgggt gacgaggagg atcgacgacc gtaggatggt cgggatacaa ttgtcccaat
     2221 cgatcgatca agggaagggc ttcgggtgta ctgttcatgc gaggttcact caccggatgg
     2281 tcccacacca gagttgcga
//
LOCUS       DATNDD010000320         4426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3246444, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000320 DATNDD010000000
VERSION     DATNDD010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246444"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /gene="priA"
                     /locus_tag="VN647_07715"
     CDS             <1..1062
                     /gene="priA"
                     /locus_tag="VN647_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWG96954.1"
                     /translation="QEVVLRPTIELVDLRNEPAGILFSHKLIRAMHEALQNHVKILLF
                     LNRKGYARTLICRDCGWVPQCDSCNVPLVYYREAGRMICRYCGKADRLPDLCPLCNTP
                     HMSPVGDGTERVEAEVRRLFRQAKIARLDGDTLRHSASAQALWEGTRSGAWDVLIGTQ
                     ALFRREPLPCYGLVGILQADSGLHVSDFRAAEHTYHLLIDAANLACPASTGGRVIIQT
                     RLPTHHTVQALISGDPDQFYNEEFAARRLLHYPPVCHLADLSVTGNDLRTVEEAAKRW
                     GAELERNAHDQEPLIVLGPVPAIRRRPKGHQRYRILVKGTVLASLSRRIHASVQKLER
                     EYRKGRIKFVIDIDPIENG"
     gene            complement(1065..1490)
                     /locus_tag="VN647_07720"
     CDS             complement(1065..1490)
                     /locus_tag="VN647_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96955.1"
                     /translation="MIFLNLSLGTLVTAVGFWLIWGGTVSPILMVGWALIVGLFLWFM
                     TESITELWAWSTLLLGLESFAWPLVLMIQLRSQEESLPESEMGTILSAVVLGLFSAVF
                     WISFSYGLFKRARKLDAAQSMEQVARVPLSRMHKKKKSM"
     gene            complement(1581..1952)
                     /locus_tag="VN647_07725"
     CDS             complement(1581..1952)
                     /locus_tag="VN647_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG96956.1"
                     /translation="MPSVLVVDDQDQVRQLIRETLEQAGYDVEEARDGKEGLDLYRTR
                     STDLVIMDILMPDQDGLETIMTLHREFPDARVIAMTGGSDAIGVLNFLGVAKMLGARR
                     TLQKPFELKVLLDTVAAELTV"
     gene            complement(1930..4062)
                     /locus_tag="VN647_07730"
     CDS             complement(1930..4062)
                     /locus_tag="VN647_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWG96957.1"
                     /translation="MTAGLFLGLLLFLSFILWIVIRQRQENVLNSTVIAAANCSVLVT
                     DATLSQHPIVYVNPAFLLLTGYAEQEVIGQRTSILTGPDTDRTSMEKLAMALQDGWAC
                     RVRLCHYRKNGTSFWNDVSLTPVKDRLGRVTAMVWTMSEVTQLGAGIKAPDRIQDEDI
                     AARGQAEQVLRDSEMRLDLAVQIGQVGFFEYDHRTDALSCSPILRDIYGVGTDEPASW
                     QRYLELVHPDDRNRVISVAQRARAATGNGRYEVKYRLIRPDGPMRHISLRSLTSFDGD
                     GSSRQPVRTIGAVVDVTDRKNVEARRRESASREAISTFAGGIAHELNNGLTAVLGFSE
                     LALPLIPTESKAHRHIRQVVAATKKSRELIQQLLAFCRQNDQGRCPLLLHSLAKESIK
                     FLRPTIPLWIEFRAQIAQTTNPISANAVQMHEMIFNLVDNALRAMQNTGGILDVQLQN
                     REFVADQIMPSGRLLAGRYVCLSVRDTSEGMDPDRVGRLFTSFLMSRDVSGRQGTGLA
                     VVHGIVTAHGGTLAVESQMGAGTTVSVYLPALSACAPSATQVGDPLPHGHECILFVDD
                     ENSVVRLGTEVLEALGYYPVVCRTAAEALEVFQVEPKRFDLLITDQTMPGMSGNGLAR
                     ECRRFRSDLPVIFCSGSKGVHHLDDACSQGVTECLPKPFTLHELAHAIRRTLDQSPAY
                     LESGGLANSGREPSRISIEVSDAVGPRG"
     gene            complement(4232..>4426)
                     /locus_tag="VN647_07735"
     CDS             complement(4232..>4426)
                     /locus_tag="VN647_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWG96958.1"
                     /translation="GTDQLSSQEERVVALVAEGKTNKEIAVALGLSDKTVKNYLANVF
                     QKLRITRRAQAAAFFVKRQV"
BASE COUNT         1034 a         1255 c         1160 g          977 t
ORIGIN      
        1 caagaagttg tccttcgacc tacgattgag ctcgttgacc ttcgcaacga gccggcagga
       61 atcctcttca gccacaaact catccgagcg atgcacgaag ccctacagaa ccatgtcaag
      121 atcctactct ttctcaatag gaaaggttat gccagaacct tgatttgtcg agactgtggc
      181 tgggttcctc agtgcgattc ctgtaacgtg ccgcttgtct attatcgtga agccggcagg
      241 atgatttgtc gttactgcgg taaggcagat cgattgccgg atctctgccc tttgtgcaat
      301 accccccata tgagtcctgt tggtgatggc accgagcgtg ttgaagccga agttcgtcgc
      361 ttattccgac aggctaaaat cgcccggctc gatggcgaca cactccggca ttcggcatcg
      421 gctcaggctc tgtgggaagg taccaggtct ggtgcatggg atgttctcat tggtacccaa
      481 gcgctcttcc gccgggagcc actgccttgc tacggcttgg tggggatcct tcaagcagat
      541 tccgggctgc atgtctcgga ctttcgcgca gcggagcaca cctatcacct actgattgat
      601 gctgccaatc tggcatgccc tgcgtctacc ggaggtcgag tcatcataca aacgcgcctt
      661 cctacacatc ataccgtcca ggccttgatc tcaggagatc ccgatcaatt ctacaatgaa
      721 gaattcgccg cacgtcgact ccttcattat ccgcccgtct gccacttggc tgatctctcg
      781 gtaaccggga atgacctgag gacggtcgaa gaggcggcta aacgctgggg tgctgagctt
      841 gaacggaacg cccatgatca ggagcctctg attgttttag gaccagtgcc ggcaatacgc
      901 aggcgtccaa aaggtcatca gcggtatcgc atcttggtaa aaggaactgt tctcgcctcc
      961 ctcagccgac ggatccatgc ttccgtccag aaactggaac gagaataccg caaggggcgt
     1021 atcaaattcg tcatcgacat cgatccgatc gagaatggat aaggctacat gctttttttc
     1081 tttttatgca tccgggaaag cggcaccctt gcaacctgct ccatggattg agccgcatcc
     1141 agcttgcgcg ctcgtttgaa caggccgtag gaaaacgaaa tccaaaatac cgccgagaag
     1201 agacccaaca cgacggctga aaggatcgtt cccatctcag attcgggaag tgattcttcc
     1261 tggctcctga gttgaatcat caataccaac ggccaggcaa agctttccag gccgagcagc
     1321 agggtagacc atgcccatag ttccgtgatg gactccgtca tgaaccataa aaacagcccc
     1381 acgatcagag cccatccgac catgaggata ggagaaacag tcccccccca tatgagccag
     1441 aacccgactg cggtaactag agttcccagg gacagattga gaaatatcat gaagggcatg
     1501 atccaggcat gacatgtgag tctgacggga ccgtgtcgtg atgtcaatgc ggagaacgtt
     1561 gagagcgaac ggtgagttgt ctagacggtc agctcggcag cgaccgtatc gagcaggact
     1621 ttcagttcga acggcttttg aagcgtccgc ctggcgccaa gcatctttgc gacgcccaaa
     1681 aagttgagaa caccgatcgc atcactcccc ccagtcatcg caatgacacg agcatcggga
     1741 aactcccggt ggagcgtcat gatggtttct aatccgtctt gatccggcat gagaatatcc
     1801 atgatgacaa gatccgtcga tctagtccga tacaggtcaa gtccttcttt cccgtcgcga
     1861 gcctcctcga cgtcataacc ggcctgctcc aacgtttccc ggatgagctg acgaacttgg
     1921 tcctgatcat caaccacgag gaccgacggc atccgacacc tcaatcgata tcctcgatgg
     1981 ttctcggccg gagttggcca gccccccaga ttccaaatag gcaggtgatt ggtccaatgt
     2041 ccgacggatg gcatgcgcca gctcgtgcaa tgtaaacggt tttgggaggc attccgtaac
     2101 accctgcgaa caggcgtcgt ccagatgatg tacacccttt gatccgctgc agaagatgac
     2161 cgggagatcc gatcgaaatc gccgacattc tcgagccagg ccattcccac tcatgccggg
     2221 catcgtctga tcagtgatca gcaaatcgaa ccgtttgggc tcaacttgaa agacttccaa
     2281 tgcttcggca gccgtcctac ataccaccgg atagtagccg agggcttcca acacctctgt
     2341 tcctaaccga acaaccgagt tttcgtcatc gacgaataag atacattcgt gtccgtgggg
     2401 aagcggatcg cccacctgag tcgccgatgg agcacaagcc gagagagcgg ggagatagac
     2461 tgataccgtc gtaccggcgc ccatttggct ctcaaccgcc agggtaccgc catgcgccgt
     2521 gacgatgccg tggacgaccg ctagtcccgt accctgcctc ccgctgacgt ctcgggacat
     2581 caaaaacgag gtgaacaatc gaccgactct gtcaggatcc atgccctcgc ttgtatcccg
     2641 aacactcagg cagacgtacc gtccagcgag gagtcgaccg gacggcatga tctgatccgc
     2701 tacaaactcc cggttctgaa gttgaacatc gaggattcct cccgtgttct gcatcgcacg
     2761 aagggcgtta tcgaccaggt tgaaaatcat ctcatgcatc tgcacggcat tggccgaaat
     2821 agggttagtc gtctgtgcaa tctgcgctct gaattcaatc cacaagggga tcgtcggacg
     2881 aaggaacttg atggattctt tcgccaacga atggagtaat aatgggcatc gaccttgatc
     2941 gttctgccta cagaaagcca gcaactgctg gatcagctcc ctggattttt tcgtcgccgc
     3001 gacgacttgt ctgatgtggc gatgcgcctt gctctcggtc ggaatcaacg gaagggccaa
     3061 ctcgctgaag ccaagtacag ccgtaagacc gttattcagt tcgtgggcga tgcctccggc
     3121 aaaggtgctg atcgcttctc tgctcgctga ctcgcgtcgc cgtgcctcga cgttcttccg
     3181 atcagtcaca tcgacgaccg ccccgatcgt acggaccggc tgtctcgagg aaccatcgcc
     3241 atcgaacgaa gtcagagaac gaagactgat gtgccgcatg gggccgtctg gtctaatgag
     3301 acggtacttg acctcatacc ggccgttgcc tgtggcagca cgggctcgtt gggccacaga
     3361 aattacccga ttccgatcat caggatgaac gagttcgagg tagcgctgcc atgaagccgg
     3421 ctcatcggta ccaacaccgt agatgtctcg caggatgggg gaacaggaaa gagcatcagt
     3481 ccggtggtcg tattcaaaga atccgacctg tcctatctgt acggcaagat caagtcgcat
     3541 ctcgctatcc cggagcacct gttcggcttg tccacgagcg gcgatatctt cgtcctggat
     3601 cctatcaggc gcttttatgc cagccccaag ttgtgtcact tcgctcatag tccaaaccat
     3661 cgccgtcacc cgacccaaac ggtctttcac cggtgtcagc gatacgtcat tccaaaatga
     3721 ggtgccgttc ttgcggtaat ggcacaaccg cacacgacag gcccatccat cttggagggc
     3781 catcgcaagt ttttccattg aggtccgatc tgtatccggg ccggtcaaga tcgacgtcct
     3841 ttgaccgatc acctcctgct cggcatagcc ggtcagcaac aggaaggccg ggtttacata
     3901 gacgatggga tgctgggaca atgtggcatc cgtcacgagc acgctacaat tagccgctgc
     3961 gatgacggtt gaattcagga cgttctcttg gcgctgtcga atcactatcc acaaaatgaa
     4021 actgaggaac aggagcaacc ctaggaagag accagcagtc atgcgggaca acctcctggt
     4081 atgtacacac ggagtggtta agaatacgaa tcagttgatg tgaacttaca tgaaatgcaa
     4141 gaggaaggcc caggtatgaa ggatagccgc aaagttaaat ttttgcacct aaaagtagta
     4201 gagccgaatg ttgtcgctcg tccttgccgt ttcacacctg ccgtttcaca aaaaaggcgg
     4261 ccgcctgcgc gcgccgcgtg atgcgaagtt tttggaatac gtttgccaaa taattcttga
     4321 cggtcttgtc gctgagtccg agcgcgacgg cgatttcctt gttggtcttt ccctcggcaa
     4381 ccagtgcaac gactcgttct tcctgagagg acagttgatc cgtacc
//
LOCUS       DATNDD010000321         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1172742, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000321 DATNDD010000000
VERSION     DATNDD010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172742"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VN647_07740"
     CDS             <1..537
                     /locus_tag="VN647_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWG96959.1"
                     /translation="SPMEYAESKEILKEIRSFLPGYGSLGPAPLPPKVDPSAVDRYLT
                     TGYEHDLATRYQPIPRMPRPDGTVRLDLTQSLFHSGKLSTRSKGLLQLEGSGRLRISP
                     LDAARFALSDGDRVRLSSTSGEMTTEVKIMERVPQGTAWFPYHFGQAAVRLFECAVDP
                     ITRVPSFRTATVSVMKVA"
     gene            584..1660
                     /gene="nuoH"
                     /locus_tag="VN647_07745"
     CDS             584..1660
                     /gene="nuoH"
                     /locus_tag="VN647_07745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWG96960.1"
                     /translation="MTEFGLRLAISLTQIAGVMGIVVVTVLILTLAERKVLGWMQDRM
                     GPMEVGPYGILQPFADAIKLFFKEDIIPAGANKFLFTVAPILCLIPAFIGFAVIPWGP
                     NQTFEIDGITVRPFVISDINIGILYILAFASLGAYGIILGGWASNSKYSLLGGLRSAA
                     QIISYELNVGLSIVGVLILAGSLSLVKITDAQAGGFWNWYLFALPAPQIFAFVVYVIS
                     SVAETNRVPFDLPEAESELVAGFFTEYSGLRFAFFFLAEYANMVLVSCVAAALFLGGW
                     NAPYPGTIMALIGLPSLAWVENTMWFAVKTYSFLFLFFWLRATLPRLRYDQLMRFGWK
                     VMLPIALGNIVVTSLAVFFYQQMK"
     gene            1675..2241
                     /gene="nuoI"
                     /locus_tag="VN647_07750"
     CDS             1675..2241
                     /gene="nuoI"
                     /locus_tag="VN647_07750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWG96961.1"
                     /translation="MISTATTKRFNLYEWFKTITFYEILVGMKATLSHLLHYRPVTVQ
                     YPHEKRTLPDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEPTKRY
                     SKEYYMDMTRCLFCGMCVDACPVDALGMTREFEWAVYDKRQLHLDKQQLLAIGDRSFP
                     VREKRLELQHPNVAFFNVAFKHVPPKPN"
BASE COUNT          487 a          673 c          651 g          578 t
ORIGIN      
        1 tcgccgatgg agtatgcgga gagcaaagag atcctgaagg aaattcgcag cttccttccc
       61 ggctatggtt cgctggggcc cgcgccgttg ccgcccaaag ttgatccctc ggccgtggat
      121 cgctatctca cgaccggata tgagcatgac ctcgcaacga ggtatcaacc tattccacga
      181 atgcccagac cggatggaac cgtgcggctg gacttgacac agagtctgtt ccattccggg
      241 aaactatcca cacgatcgaa ggggttgtta caactcgaag ggagcggccg gctccgtatc
      301 agtccgcttg acgccgcgcg ctttgccttg tcggatgggg atcgagtccg cctttccagc
      361 acctccgggg aaatgacgac cgaggtcaag ataatggagc gcgtcccaca aggaacggcg
      421 tggttcccat atcactttgg tcaagctgcc gtccgactat ttgaatgcgc cgtggacccg
      481 atcacccgtg taccgtcatt tcggacagcg acggtgtccg tcatgaaggt ggcgtgatga
      541 ctctcttcat ccctgatggt tatgctaatc gaggagtcca attgtgactg aattcgggtt
      601 acgtctcgct atctcgctga cccagatcgc cggagtcatg ggcatcgtgg tcgtcacggt
      661 gctcatcctc actctggcgg aacgaaaagt tctggggtgg atgcaggacc gcatgggtcc
      721 gatggaagtg gggccctacg gcattctcca gccgtttgcg gatgccatca agctcttctt
      781 taaggaagac attatccctg ccggcgccaa caagttcctt ttcaccgttg cgccgattct
      841 ttgtttaatt cccgcgttca tcggatttgc cgtcattccg tggggtccca accagacgtt
      901 tgagatcgac ggcatcaccg taagaccgtt tgtgatcagc gacatcaata tcggcattct
      961 gtacatctta gcgttcgcct cgctcggggc ttacggcatc atcctggggg gatgggcttc
     1021 caacagcaaa tactcattgc tcggcgggct acgatcggcg gcacaaatca tcagttacga
     1081 gctcaacgtc ggtttgtcca tcgtcggggt gttgattttg gccggttcac tcagccttgt
     1141 gaaaatcacc gatgcccagg ccggaggctt ttggaattgg tatctctttg cgttaccggc
     1201 cccgcaaatt tttgcgtttg tcgtctacgt gatctcatcg gtggctgaaa ccaaccgggt
     1261 cccgttcgat ctgccggaag cggagagcga actcgtcgca ggcttcttca ctgaatacag
     1321 cggcctcaga ttcgcgttct ttttcctcgc cgagtacgcc aacatggtgt tagtgtcctg
     1381 tgtagccgcc gcgctgtttc tcggcggatg gaacgcaccg taccctggaa cgattatggc
     1441 gctcatcggt ttgccgtcct tggcctgggt cgagaacacg atgtggtttg cggtcaagac
     1501 ctactctttc ttgttcctct tcttctggct tcgagccacg ttaccgagac tgcgatacga
     1561 tcagctgatg agattcggct ggaaggtaat gctgcccatc gcgttgggta acatcgtcgt
     1621 gacgtccctc gcggtatttt tctaccaaca gatgaagtag taggcgaggc ctatatgata
     1681 tctacggcga ccaccaagcg tttcaacctg tacgaatggt tcaaaacgat cacattctac
     1741 gagattctcg tcggcatgaa ggcgaccttg tcgcatctgc tccattaccg tcccgtaacc
     1801 gtgcaatacc ctcatgaaaa acgcacactg ccggacaatt accgaggcat gctcgcgctg
     1861 ctccgatacg atgatggaac cgagaagtgc gtgggatgcg atctctgtga agcagcctgt
     1921 ccgtctcgcg tcatccgggt cgtcagcgcc gaggtgccgg gtgaaccgac taagcgttac
     1981 tccaaagaat attacatgga catgacccgt tgcttgttct gcgggatgtg cgtggatgcc
     2041 tgccctgtcg atgcgctggg catgacgaga gagtttgagt gggcggtgta cgacaagcgc
     2101 cagctccacc tggataaaca acaattgctc gcgatcggcg accgctcatt tccagtccgt
     2161 gagaaacgcc tggagctgca acatccaaac gtcgcgttct tcaatgtggc attcaagcac
     2221 gtgccaccca aaccgaactg actctcaaag aattctcatc ggacgagcga tgactgcaac
     2281 accggagcat tagcctgacc ctgatcgccc tctaactcag gtaacgtctc atgtcgcagc
     2341 tgttttttgg atacttcgcc gggatgatcg ccgtcacctc catgctggt
//
LOCUS       DATNDD010000322         5844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2651160, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000322 DATNDD010000000
VERSION     DATNDD010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651160"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VN647_07755"
     CDS             complement(<1..207)
                     /locus_tag="VN647_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96962.1"
                     /translation="MPLTENTFSIVSVNGPFREPREQVFSYDYSIQRSTWVTPHGVRV
                     KVSIPDELEVLKRRLFGPVVGSPGQ"
     gene            472..1320
                     /locus_tag="VN647_07760"
     CDS             472..1320
                     /locus_tag="VN647_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96963.1"
                     /translation="MAKKGYPGLVLVPPSDRTILRRRLLRWALLIIALIWCASTLPLW
                     PPVPGDRENCQPGVGQEGSNSLAENCGQRQRVTEPSPNQSEFSLTTVSQAEGLGESRN
                     PPPSPTPDPDRSATQNDRDRDSQPKSPDPIDHPSGHDVAPLGNEKSTSSLPTAKSVQN
                     PDADVRLAEQGDAFAQYRLGRYYARRDGPRTPESVSWYKKASHGLHRLAGTGNGQAMY
                     VLGVMYAYGRGVTRDTEQARHWLTQAVQQKVTAAQPVLASLAVQPHTTPNSKAAEQAK
                     NLKQQN"
     gene            1383..1796
                     /locus_tag="VN647_07765"
     CDS             1383..1796
                     /locus_tag="VN647_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWG96964.1"
                     /translation="MNGIARIKTKTPSPYKLTHIEVDTHDTKTFRFELRADATLDMLP
                     GDFLYIHTTINGKHVKRPYTPSSLVGTTGFFDLTVKRYAAGSVSRYLHDQLIGDTVLM
                     SGPNTGGSGNATSKTIRTFIATTSWNSRLPGGKVA"
     gene            2165..3427
                     /locus_tag="VN647_07770"
     CDS             2165..3427
                     /locus_tag="VN647_07770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96965.1"
                     /translation="MSQGSLVSQATRKTAAKVLSHGIGRLAERVRANGGQPLDLILPD
                     GSRLNFGQEPRVVLAIRDPNLLSALTHPTLGLLGEAFVDGRIDIDGDIISLIASVERL
                     VAAGGSPATVRIPASSACHTPKQDFDDIKHHYDVSNDFYRLWLDDRMVYSCAYFRTGE
                     ETIDDAQEAKLDHICRKLRLQPGERFLDIGCGWGGLVIHAAQRYGVHAVGITLSDNQF
                     ALAKERIKTSGLKRQIRVLLLDYRDAPERLGESTFDKISSIGMFEHVGRDNFHLYFGI
                     VRRLLRDRGLFMNHGITSPDADGRTVGSGVSEFMDKYVFPHTELPHLHSVIREMAKQN
                     FEVYDVESLRPHYARTLAHWSRRLERQLAAARHFVGERTLRIWRTYLAGSSLAFSQGW
                     LTVYQVLASRQEAGGSTELPLTRAWMYH"
     gene            complement(3484..4932)
                     /gene="hemG"
                     /locus_tag="VN647_07775"
     CDS             complement(3484..4932)
                     /gene="hemG"
                     /locus_tag="VN647_07775"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HWG96966.1"
                     /translation="MTRLRTVVIVGGGISGLATAFSLQEMATTAGLPIRCTILESESS
                     WGGKIVTHRVGEIVTEAGPDSFLSQKQAGLDLCLKLGLSDQLINTNETAKKASVLYAG
                     RLHDLPEGLLSFVPKQLGSFFRSGLLTWTGLARMGLEAAVPPGPSTGDESLAAFLRRR
                     FGVQAFTRVLEPLMAGIYAGDAEQMSLRATFPRFFELEQQHGSIVRGMMAAKKAVSSV
                     SIDQPRRTMFVSLKNGLSDLVTALASRLTQQGVELRVGARVEALRVRSHEIGRWMYDL
                     ILQDGSALSADGVVLAAPAYVSAELLRPLTPIAGGLLDMIPYASTATIAMAFPRTLTS
                     AIEGFGFIVPRAEQRHLIAATWTSLKWSYRAPADQLLVRCYVGGVGREDILQLDDDAL
                     TATVREELAAVCGIRVRPSYAEVNRWWKAMPQYTLGHLNRLAQLEAALSRYPGLVLTG
                     AGYRGVGIPDCIRDGAVAGERVIQNLLGETRR"
     gene            complement(4929..>5844)
                     /gene="hemH"
                     /locus_tag="VN647_07780"
     CDS             complement(4929..>5844)
                     /gene="hemH"
                     /locus_tag="VN647_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWG96967.1"
                     /translation="MAMGGPDCLENVEPFLRDVRGGRPTPPALVEEIRERYRVTGGKS
                     PAVSIAQEVAKKLEQQLNVAAHRRYKVYVGLRHWHPFIRETYSDLLKESPEQIIGVCM
                     APQQSSLSTGAYRKKVEEARAEFGDRTDVTYVGSWNRHSRLIAAIVDNIRKGLFAFPA
                     DVRASVPVLFTAHSLPERIVAMKDPYPEEVKGTVEAVTQFLGNQPIYFAYQSQGRSNE
                     PWLGPTVESMLEAIQLAGHRDVLVAPIGFICDHVETLFDIDIELKQLATNKGLHLERI
                     AMLNDSPALIETLHDVLTAHESSLCIQS"
BASE COUNT         1344 a         1757 c         1528 g         1215 t
ORIGIN      
        1 ctggcccggc gagccgacca ccggcccgaa gagacgacgt ttaagcacct ccagctcatc
       61 cggtatggaa accttcacgc gcacgccatg gggcgtcacc caggtggacc gctgaatcga
      121 atagtcatag gaaaagacct gctctcgcgg ctcacgaaat gggccgttga cgctgacgat
      181 tgaaaaggtg ttttcagtta atggcatggt ttgctcgctt tcctgggtga caatggtgcc
      241 ctactagaat cgacctacta aacctactat aagagcttga agttgggggg gacaagagga
      301 tatttatact ggtagaagcg caactgatat aagacggata gggcgaccgt ttgcttagta
      361 ttcagaactc cgcagttccc ccaatgctac agcagcggcg ggattccgga gcacgagaca
      421 gctttttgac acactgatat actccgacca agggttcctt cgggagaagg catggccaag
      481 aaaggctatc ctgggctcgt cttggtaccg cccagcgatc gaacgatcct gaggcgccga
      541 ctgcttcgtt gggctttgtt gatcatcgcg ctgatctggt gtgcatcgac actccccctc
      601 tggcctcccg tccccggcga cagggagaac tgtcaacccg gtgtcgggca ggaaggctcg
      661 aacagtctcg ctgaaaactg cggccaacgg caacgggtca cggagccgtc acccaatcaa
      721 agtgagttca gccttacgac ggtttcacag gctgaaggcc tgggcgagtc ccggaatccc
      781 ccgccttcgc ctacccctga tcccgatcgg tccgcaaccc agaacgatcg tgaccgggac
      841 tcccagccca aatcacccga tccgatcgac cacccgtctg gtcacgatgt ggcaccatta
      901 ggcaacgaga aatcaacatc aagtctgccg accgcaaaat ctgtgcaaaa tccagatgcc
      961 gacgttcgcc ttgccgaaca aggcgatgca tttgcccagt atcgcctcgg acgttattac
     1021 gcacggcgtg acggaccacg gacgccggag tccgtcagtt ggtacaagaa agcttctcac
     1081 ggtctacacc gcctggctgg gaccggtaat ggacaggcaa tgtacgtcct cggggtcatg
     1141 tatgcctatg ggcgcggagt gaccagggat acggagcaag cgcgacactg gttgacgcaa
     1201 gctgtgcaac agaaggtcac agcggctcag ccggttcttg cgagtctggc agtgcaacct
     1261 cataccactc ccaattcgaa agcagccgaa caggcaaaaa acctcaaaca acaaaactga
     1321 aacaatccga ccaccccggt attgcgccgg ttgatacaac cccataatat acatcctccg
     1381 ctatgaacgg catcgctcgc atcaaaacca aaacgccctc tccgtacaag ctgactcaca
     1441 tcgaggtgga tacgcacgac acgaagacgt tccgatttga gcttcgtgcc gatgccactc
     1501 tggatatgtt gcccggcgat tttctctaca tccatacgac gattaacggc aagcacgtca
     1561 agcggccata cactccctca tcgcttgtcg gcaccaccgg gttcttcgat ctgacagtca
     1621 agcgatatgc tgctggctcc gtctcgagat acctgcacga tcagctgatc ggagatacgg
     1681 tattgatgag cggcccgaac accggcggga gtgggaatgc aacgtccaaa actatccgaa
     1741 ctttcattgc caccacgtct tggaacagcc gcctcccggg tgggaaggtg gcataggtcg
     1801 cgtcacccct gaaattcttc ggcgccatct tcctgctccg ggctccgaca cctgtgtttt
     1861 tctctgcggg ccgccgccga tggccgactc attggaggcc gtgctgaaag aactgggcta
     1921 tccccggcaa gccattatcc tcccctgagg aacatcctag ttctcatcct catcagtatc
     1981 cgaatattgt actctcagag tatcttagtc ggttgaagta atacttcaca tctctttggc
     2041 ataagagcat ctttgacgtt cgcttgagcc aaatttatcg aacggtgagt attccagggc
     2101 ttggcctcca actaataccc gtatttgatc aagaatcgaa gagtgcagcc caaaggaggt
     2161 tatcatgtct cagggcagtc tcgtctcgca ggcaacccga aagaccgcag ccaaggtact
     2221 aagtcatggt attggacggc tggcggaaag agtccgggca aacggcggcc agccgctcga
     2281 cctcattctt cccgacggtt cgcgtctcaa tttcggccag gaacctcgcg tggtactcgc
     2341 aatccgcgat ccgaacttgc tgtcggcatt aacccatccc acgctcggat tgctcggaga
     2401 agcgttcgtc gatgggcgca tcgatattga cggcgacatc atctccctga tcgccagcgt
     2461 tgagcgcctc gtggcggccg gcggctcacc ggcgacggta cgaatcccag cttcatccgc
     2521 atgccatact cccaaacagg atttcgacga tattaaacat cactacgacg tgagcaacga
     2581 tttttaccga ctctggctgg atgaccgtat ggtctattct tgcgcgtatt ttcgaaccgg
     2641 ggaggaaacg atcgacgacg cgcaagaggc caagctcgat cacatctgcc gcaaactacg
     2701 ccttcaaccc ggtgaacgtt tcctcgacat cggctgcggc tggggcgggc tggtcattca
     2761 tgccgctcag cgttacgggg ttcacgcggt cggaatcacg ctctcggata atcagtttgc
     2821 cttggccaag gagcgtatca agacgtcggg ccttaaacga cagatcaggg tactcctgct
     2881 ggactatcgc gacgcgccgg aaagattagg ggagagcacc ttcgacaaaa tttccagtat
     2941 cggcatgttt gagcatgtcg gccgagataa ttttcacctt tacttcggca ttgtacggcg
     3001 cctgctgcgc gaccgagggc tgttcatgaa ccacggcatc acctcaccgg atgccgacgg
     3061 acgcacggtc ggctccggag tgagcgagtt tatggacaag tacgtgttcc cacataccga
     3121 actcccccat ctccactcgg tcatccgaga gatggcgaaa cagaacttcg aggtctatga
     3181 cgtcgaaagc ctacgaccgc actatgctcg gacactcgca cactggtcac gcagactgga
     3241 aagacagctc gccgcagccc gtcatttcgt cggcgaacga acgcttcgga tatggcgcac
     3301 ctatctcgca ggctcatcac tggcattttc gcagggctgg ctgaccgtct atcaggtgct
     3361 ggccagccgc caggaagccg gcggctcgac tgagctgccg ttgaccagag cctggatgta
     3421 ccactgaaac tcaacctgca tccccgacct tagccttagg ctcagcttat ccccatggtg
     3481 agatcatcgt ctggtttcgc ctaagaggtt ctgtataacc ctctcgcctg ccacagctcc
     3541 atcacgaatg cagtcgggta tgccgactcc gcgataacca gctccggtaa gaacaagtcc
     3601 gggatagcgg ctcagtgccg cctccagttg tgccaaccga ttcagatggc cgagcgtata
     3661 ctgcggcatc gccttccacc atcgattcac ttcggcataa ctcggtctta ctctgatgcc
     3721 gcatacagcg gcgagttctt ctctgactgt tgccgtcaat gcatcgtcat ccagctgaag
     3781 aatatcttcc cgccctactc caccgacata gcagcgcacc aacagctgat ctgccggagc
     3841 ccgatacgac cacttgagcg aagtccacgt tgcggcaatt aaatgccgct gctccgctcg
     3901 cggcacgata aatccgaacc cttcgatggc actcgtcaga gttcgtggaa atgccatcgc
     3961 aatggtggca gtcgaagcat aaggaatcat gtccagcaaa ccgccggcta tcggcgtcaa
     4021 tgggcgtaag agttcagcgg aaacataggc aggtgcggca aggaccacgc cgtctgcgga
     4081 aagcgccgat ccatcttgca aaataaggtc gtacatccag cggccgatct catgcgacct
     4141 cactctgagg gcctcgacac gagcccccac acgcagctcg accccctgct gcgtcagccg
     4201 actcgccaag gcagtcacca aatcgctgag accgttcttt aaactgacga acatcgtccg
     4261 tctgggctga tctatcgata ctgatgaaac cgctttcttg gcggccatca tgccgcgaac
     4321 gatactgcca tgctgttgct ccaactcaaa aaaccgcggg aacgtggcgc gcagactcat
     4381 ttgctcggca tcacccgcgt aaattccggc catcaacggt tccaagactc gcgtaaacgc
     4441 ttgcacccca aaccgacggc gaagaaaggc ggccaacgat tcatcgcccg ttgaggggcc
     4501 tggtggaaca gcggcctcca gccccatacg cgccaaccct gtccaggtga gcagaccgct
     4561 ccgaaagaaa gaccccagtt gtttcggaac gaaagagagc agtccctcgg gcaagtcatg
     4621 gagccgtcct gcatatagca cgctcgcctt cttggccgtt tcattcgtat tgatgagttg
     4681 atcggaaagg ccaagcttca ggcagagatc gaggccggcc tgtttttggg aaagaaagga
     4741 gtccggtccg gcttcagtca caatctcgcc gacccggtgc gtgacgatct ttcctcccca
     4801 ggacgattcg gattcgagga tcgtgcagcg aatgggaagg ccggccgtgg tcgccatctc
     4861 ttgtagtgaa aaagcagtgg ccaagccgga aataccgccg cccactatca cgactgtgcg
     4921 tagtcgagtc acgactggat gcagagagag gattcgtgtg ccgtcaagac gtcgtgtaac
     4981 gtctcgatga gtgcgggtga gtcattcagc atggctatac gctcaaggtg tagaccctta
     5041 ttggtcgcca actgcttcaa ttcaatatca atatcaaaga gagtctccac atggtcacaa
     5101 atgaagccaa tgggcgccac cagcacatct cggtgtcccg ccagctgaat agcttccaac
     5161 attgattcca cggtaggtcc caaccacggt tcgtttgatc gcccctgact ttgataggca
     5221 aagtagatcg gttggttacc cagaaactga gtcactgctt cgaccgtacc cttcacttcc
     5281 tcggggtaag gatccttcat ggctacgatt cgttccggca gactatgagc agtaaacagc
     5341 acgggcaccg atgcccgcac gtcggcagga aacgcgaata accccttccg aatgttgtcg
     5401 acgatcgcag caatcagccg agaatgtcgg ttccaactac cgacatatgt cacatcggtc
     5461 cgatccccaa actcggcacg ggcctcttcg actttcttcc gataggctcc ggtactcaag
     5521 gaggactgtt gcggggccat acagaccccg atgatctgtt ccggcgattc tttcaataga
     5581 tcggaatacg tttctctaat gaagggatgc cagtgccgca aaccgacgta caccttgtaa
     5641 cgtctgtgag cagccacatt caattgttgc tcgagtttct tcgcaacctc ctgcgcaatg
     5701 gagacagccg gtgatttgcc tccggtgact cggtatcgtt cgcgaatctc ctccacgagc
     5761 gctggaggtg tcggtcgccc ccctcgaaca tcgcgcaaaa acggctcgac gttctccaga
     5821 caatcgggcc cacccatcgc catg
//
LOCUS       DATNDD010000323         7762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4149430, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000323 DATNDD010000000
VERSION     DATNDD010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149430"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1660
                     /locus_tag="VN647_07785"
     CDS             134..1660
                     /locus_tag="VN647_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWG96968.1"
                     /translation="MNKNVTGTQSIWMGAGVESTSPPLDRSLETDVCIVGAGIAGLST
                     AYSLSCEGKSVVVLDDGPVAGGMTERTTAHLSNAIDEGYVTIERLHGEQGARLVADSH
                     TAAINRIETIIAAERLACDFERVDGFLFPAPGQQLERLEEERLAALRAGLTDVVRVER
                     PSGPVLEPGGCLRFPNQAQFHPLKFMAGLAQAIARCGGRIFTNIHAETVKDGKQVTIE
                     TRQGHIIRAQSLVVATNTPINDMFVVHTKQASYTTYVIGAGVPKDSVPTALYWDQADP
                     YHYARIQKDASTDGHEVLIVGGEDHKTGQADDGDERCQRLERWARKRFPMMEQIKYRW
                     SGQVMEPMDGLALIGRNPGDASNVYVATGDSGMGMTHGMIAGMLITDLIQGRTNGWAE
                     LYDPSRKSLKSIGTFVQENVNVAAQYADWISSGDIESEDHIPKDTGAVLRNGAQKMAV
                     YRDEDGQLHRRSAVCPHLKCIVAWDSMEHTWNCPCHGSRFDAYGKVINGPANSDLSQI
                     "
     gene            1975..2208
                     /locus_tag="VN647_07790"
     CDS             1975..2208
                     /locus_tag="VN647_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWG96969.1"
                     /translation="MNADTFKGKWNQFKGELKQQWGKFTDDDLMLIEGNYDKFKGKVQ
                     ERYGDRKEELETWAAKWYGDTAKQVGEAGSSRR"
     gene            2432..3523
                     /locus_tag="VN647_07795"
                     /pseudo
     CDS             2432..3523
                     /locus_tag="VN647_07795"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
     gene            3587..4372
                     /locus_tag="VN647_07800"
     CDS             3587..4372
                     /locus_tag="VN647_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG96970.1"
                     /translation="MKLVLVFAPILLTLLVLFDRMPSSAQITLWETYQSAGHQAIEDG
                     RVADAERLLLAAAQYIETVSPDDPRPADTLNDLGVLYGMQNRDIEAEPLFEKASAINE
                     KAFGRQHPSVVLALQNLSVIYASQNKFSEAHRAARESLEISLHLFGIYHPRIGSTCRT
                     LATVYALEGKYEEAERFAEKSITVFENTLGERHPETAQSLEMMVRLMWSTHREREAQR
                     LEARLNTIRQSSGSESESAEDTDRQGIKNPNTLREDSRAARRE"
     gene            4418..4585
                     /locus_tag="VN647_07805"
     CDS             4418..4585
                     /locus_tag="VN647_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HWG96971.1"
                     /translation="MLSWAITFLIVAIIAGVLGLSGIAGTATNIAYILFVIFLIVAVI
                     GLVMGRRPPVT"
     gene            complement(4763..5239)
                     /locus_tag="VN647_07810"
     CDS             complement(4763..5239)
                     /locus_tag="VN647_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96972.1"
                     /translation="MNIRKQTSSSKTEPVSQSARETSAYLPGLRMMCMSFSKEWDVSK
                     KLIYLGLALLIWHTGNLGYAVQTTETADSSTTGEDLQSHHVVTGTLDQLDFDTGKGLL
                     KTDLGKSVFFAMVRPELFRRLSLGQRVTIGMNEQGQAVKVMEIPPSELPIPPSAAQ"
     gene            6200..7657
                     /locus_tag="VN647_07815"
     CDS             6200..7657
                     /locus_tag="VN647_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWG96973.1"
                     /translation="MDRPTILIVDDDAETQALLRDVLSKEEYDVDTAADGKIALDMVT
                     RLGPDLVISDINMPGLNGLALLEELRSRGHDMPVILMTAYGSLKTAVDGIRGGAFDYL
                     SKPFIMDDVRLVIHRALEHTQLSRQNQQLKEQLRDRYRFDNLIGSSPAVVSVYKSIAR
                     VAQTDSTVLLEGESGTGKELIARAIHANSARSAGPFVTVDGGALTETLLESELFGHER
                     GAFTGAVGTKKGLLEKAHLGTCFLDEVADLSPALQGKLLRVIQEKEFRRVGSTMTTTV
                     DVRIIAASKKDLSSLVQAGTFREDLYYRLNVVTIHIPPLRERMEDVPLLAQHFVQTYG
                     SAKPKPVTGMSAEAIAVLTHYWWPGNVRELEHAIERAIALTPHPIIYPEDLPHAVHTA
                     TVQTAAQAQGWVTLEELEREHIVRVLKHHHHDLGRSAAILGIHRKTLLRKLRQYGLAD
                     GQRTRYPPPDILSTPVRSGPSPAISNPRVLDPSRS"
BASE COUNT         1764 a         2083 c         2128 g         1787 t
ORIGIN      
        1 aataccggca cgaccattgt gacgttcctg atggttttct tgatcgtaga gcaccaaatc
       61 tgaacacgga gtcgatattc tcaaggggat tcccttgttg tgcggtagcg atcgatatgg
      121 cccactacac aatatgaata agaatgtgac tggaactcag tcgatctgga tgggcgcagg
      181 ggtggaatct acatcacctc cactcgatcg ctccttggaa acggatgtgt gcatcgtcgg
      241 ggccggaata gccggcctgt caaccgccta ttctctgtca tgtgagggca aatcggtggt
      301 tgttcttgac gacggcccgg tcgccggagg gatgacagaa cgtaccacgg cacatctatc
      361 taatgccatt gacgaaggat atgtgacgat cgagcggctt cacggtgaac aaggtgcccg
      421 tctagttgcc gacagccata ctgcggcgat caatcgaatc gaaaccatca tcgcggccga
      481 acgtcttgcg tgcgacttcg agcgggtcga tggctttctc tttccggcgc ccggtcagca
      541 gctggaacga ctggaagaag aacggttggc ggctcttcgc gccggcctta ccgacgtcgt
      601 tcgggttgaa cggccttctg gtccggtcct ggaaccgggc ggatgtctcc gctttccaaa
      661 ccaagctcaa tttcatccat taaagtttat ggccggcctt gcgcaggcga ttgcacgatg
      721 tggtggacgg atcttcacga acatccatgc ggaaaccgtg aaggatggga aacaggttac
      781 aatcgaaact cgacagggcc atatcatccg cgcacaatcg cttgtcgttg ccaccaatac
      841 acctattaac gatatgttcg tggtgcacac caagcaagcg tcctatacca cctatgtgat
      901 cggagccggc gtgccgaagg attccgtgcc taccgccttg tattgggacc aagcagaccc
      961 ctaccactat gcgagaattc agaaggacgc gtcaaccgat ggtcatgagg tgttgatcgt
     1021 gggtggtgaa gaccacaaaa ccggccaggc ggacgatgga gacgagcgtt gtcaacgatt
     1081 ggaacgctgg gcccgcaaac ggtttcccat gatggaacag ataaagtacc gatggtcggg
     1141 gcaggtcatg gagccgatgg atggcctcgc attaatcggt cgaaaccccg gtgatgcatc
     1201 gaatgtctat gtcgccaccg gcgactccgg catgggaatg actcatggca tgattgccgg
     1261 catgctcatt acggatctca ttcagggacg caccaatggc tgggccgagt tgtacgatcc
     1321 ctcccgtaaa tcattgaaat cgatcggcac gtttgttcaa gaaaacgtga atgtcgcggc
     1381 gcagtatgcg gactggattt cgtccggtga cattgaatca gaagatcaca ttcctaaaga
     1441 caccggagcg gtacttcgga acggcgcgca gaagatggcc gtgtatcgcg atgaggacgg
     1501 acagttacat cggcgctcag ccgtgtgccc tcacctgaag tgtattgttg catgggattc
     1561 catggagcac acctggaact gtccatgcca tgggtcccgc ttcgacgcgt atggaaaagt
     1621 gataaatggg cccgcgaaca gcgatctttc tcaaatttag acgtccggtt gttcgatact
     1681 gatcttcctc attacatcgt cggaactctc ttccgttcgc tgatcttccc gggtcagaga
     1741 ggctctgctg tatcgatcat cctagcatgt cgtcactcga ccgcctgctt agcgtggggc
     1801 cataggtaaa tttcacgtaa gcattttcag ccgcatctgc tcccttccaa cccaaggaac
     1861 ccatttcgga tcggtttact agggaacagg ctagtcgatg ccggcctcgt catgcgttac
     1921 acaatacatg ggaatggaga gagggaacag ttaatcacca accaggaggc tgatatgaac
     1981 gcggacacat tcaagggcaa atggaatcaa ttcaagggag agctcaagca acaatggggc
     2041 aagtttaccg atgatgatct gatgctgatc gaagggaact atgacaaatt caaaggtaaa
     2101 gtccaagaac gctacggcga ccgcaaggag gagctcgaaa cttgggcggc caaatggtac
     2161 ggcgacaccg caaagcaggt tggggaagcc ggctcctccc gaagataacg gcccgactga
     2221 caatcagaga tccgggagtc cggcaacaag attaagagga caagaaattc gatgataatg
     2281 gccatatcgt cacgatcggc ttaaacgttc gcttctagca gttatgccgt cttccgcaga
     2341 gcccggacgc cgacggaagg cctgcacgag gttatccgtc ggctcagggt ctcccatacg
     2401 atttctattt catgtattca agcacttccc aatgtgtcta ctcgctcccg ccttcctctc
     2461 gtgcgtctct ctactctgtg cacatccggc atggtcgcaa gtcaccggtg acgaatatat
     2521 tgccggtttt gccacggcgg tcttaagaca tgagttcaaa gcgaccgatt cgacgctgca
     2581 ggtacacaat ggcgtgatcc gcgtcaacgc gcgatcgctc ggcggactcg accgtgaaaa
     2641 ggtcaaacgc gcattggcgg gcattgcagg tgtcaaagat gttgtgctga tagaggacga
     2701 agcaatcgct gccgcgccgc cgcccgctca tggtgtccag gtcgacattc ctgggacgga
     2761 atccagcttc ctgccgcgag gcctcctgtt tgctccgctt cacgcagatc ctcgatggcc
     2821 tcatttctct gcggcctatc gcgggtaccg taccaataat ctcacggcga ctttcggagg
     2881 aaatttcggc gaaacgtttt cgatctatcg aaatcgcgct ccgttgaacg gccagtggga
     2941 attcgtgatc caggctggtc tcttcagcct gttcaacctg aactccgtgt cgctgaacct
     3001 catcaatgcc gactataccg tgggagtgct gacgacctat cgctccggta atttttcggg
     3061 tatttttcgc ctgcaccatc aaagctccca tttgggcgat gaatttctgg ttgcgaatcc
     3121 gggggtccga cggatcaatc taagttatta agaagtcgac ataaaactct cctatgaatt
     3181 gtccaactgg gtacgcctat acggtggcgg cggatatata gttcatcgcg atccggtcga
     3241 cgtaaaacct gttacgacac aatggggtct tgagttgacc ggtcccatct ccttctttga
     3301 cggttctatt aggccggtct gctatgcgga ctttcaggtc aacgagcgtg cgaattggtc
     3361 ggtcgctcaa tccgtcatgg cgggcctgag attcgaaaat gcacgaatcg gcaatcgcca
     3421 attacaactc ttggctgaat atttcgcagg tccctcgccc aacggccaat tctatgtccg
     3481 acatgagcac tggttcggca tcggtcttca cttctatttc tgaccgcgtc tgtatcaagg
     3541 gaccttccga gtatcgtgcg tgggtcccga acgatagggt atcagtatga agctcgtact
     3601 cgtatttgca ccgatattgc tcacactgct cgtactgttc gaccgcatgc cgagcagtgc
     3661 acagataaca ttgtgggaga cgtatcagtc ggcagggcac caagccatag aagatgggcg
     3721 tgtcgcggat gcggaacgct tattgctcgc cgcggcccaa tacatagaga ccgtcagtcc
     3781 tgacgatccc cgcccggccg atacgctcaa cgacctgggc gtgttgtacg gcatgcaaaa
     3841 tcgagacatt gaagccgagc cgctctttga gaaggcatcg gccatcaatg aaaaggcatt
     3901 cggacggcaa catccttccg tcgttttggc gttgcaaaat ctcagtgtga tttacgcctc
     3961 gcagaataag ttctccgagg cacacagggc ggcgcgcgaa agtttagaaa taagtcttca
     4021 cctattcgga atataccatc cccgtattgg atcgacctgc cgaacactgg ccacggtcta
     4081 tgcactggaa ggcaagtatg aagaggctga acgctttgca gaaaaatcga tcactgtctt
     4141 tgaaaacacg ttgggcgaac gccacccgga aactgctcag agcttggaaa tgatggtccg
     4201 gctcatgtgg tcgacccatc gagaacgcga ggctcagcga ctcgaggccc gcctcaatac
     4261 catccgccag agctcgggct ccgaatctga atcggctgag gataccgatc gccaaggcat
     4321 caagaatcca aacaccttgc gcgaggactc acgtgccgcc cgtagagagt gagtgatacc
     4381 gtcgtttgac cggttcttac ttttgaggag gtgtaccatg ctgagctggg ctataacgtt
     4441 tttgatcgtc gcgatcattg ccggtgtttt aggcctctcg ggaattgcgg gtacggccac
     4501 gaacatcgcc tatattcttt ttgtcatctt tctaatcgtt gctgtcatag gcctggtgat
     4561 gggccgacga ccgccggtta cgtaaagatt tccggcgcgt ttacccgaaa cccgaaggta
     4621 attgaacaca tcgggtaaac gcgtgaggag atgatccaag cagtccggct ctctcgcccg
     4681 atcacattcc ttgcgacgtt tgtcatcgca aaccttacac gtgtgaatcg tgaatccagt
     4741 cttccttgtg agttgagaat agctactgtg cagctgatgg cgggatcgga agttcgcttg
     4801 gtggaatttc catgaccttc acggcctggc cctgttcatt cattccgatc gtcacacgtt
     4861 gtcccagtga caaccgtctg aataactccg gcctgaccat cgcaaagaat acagatttac
     4921 caagatcggt tttgagcaac cccttgccgg tgtcgaaatc aagctggtcc aaagtaccgg
     4981 tgacgacgtg gtgggattga agatcttcgc cagtggtgga actgtcggcg gtttccgtcg
     5041 tttgtacggc atatcccagg ttgccggtat gccatatcag caacgcaaga ccaagataga
     5101 tcagtttctt cgatacgtcc cattccttac tgaatgacat gcacatcatg cggaggccgg
     5161 gtaggtatgc gcttgtttcg cgggccgatt gggagacagg ctcggtttta gaactcgagg
     5221 tctgcttcct gatattcatc gtctcgcctc cttgtcgctt gtcacatgga cctctgcctc
     5281 cactagtgac ttcatagcat tcggatactg ggaaagcctc taggcaacca tggatgctgg
     5341 agagacagag ttatttctgt atgcatgtgc ttcatgccgc agtggttggg gtctgcctag
     5401 cggccgtcga tccgcgcctt catacgatga agcaccaagg ggtatctcca gtgggcaccc
     5461 attgcgtctt ggatcacact accgcctaaa agatcggcgc cagaaagcca tgcgtgacac
     5521 atccggtcgt gacaatttgt gctcatggtt attcaaggcc ggtgttctga tcgcctggtg
     5581 ccgtcaccgc cccaccagac gcaagacgta aagggtgatg gcggtgaatg acacctggtt
     5641 acgcgcgctc ggacgtcttc atgcatgcca ccgtgctaca gtgtcttgtc gtcttcgtga
     5701 atgattcgtg tcgcgccttg agcttggttt actggaatcg atgggttgaa tgttactgat
     5761 cgctccgcaa ttcacgcaac gggagcccaa acattgccac gcaaagtact cggtggcgcc
     5821 gtagaaataa tcatgtactt taagtccacc gcaccgttca caaaccatgg cggcacctct
     5881 tttgttgatt gtgaaacgac ccgttccatg atcgagtcga gcatgtatct gttgccatgg
     5941 actaagcacg aacactgcca ggcagattcg tcgaacgaag ttgttgacaa ttcagcgttt
     6001 cgagcttgcc gcttggctgc agatccgcac cgacgaatgg gacaacttgt tcctgctcat
     6061 cgacgacatg tcatgagccg tcttgatcgg cgtatcgatg acgctgcgca cagccttcgg
     6121 gtgtgtccga atgatgccca gggaccgaca tatgcgaaga tcgcggccag gccggccgtt
     6181 gccataacgg ggtgatgaca tggaccgtcc cactattttg atcgtggatg atgacgccga
     6241 aacacaggcg ttgctccgag atgttttgtc gaaagaagag tacgacgtcg acaccgcggc
     6301 cgacggaaag atcgccctgg atatggtcac tcggctgggg ccggacctcg tgatatccga
     6361 tatcaatatg cccggactca acggcctcgc tctactcgag gagttacgtt ctcgcggaca
     6421 cgacatgccc gtcattctta tgacggccta cggtagcctg aaaacggcgg tggacggcat
     6481 ccgaggaggg gcgttcgact atctcagtaa accgttcatc atggacgatg tgcggttggt
     6541 gatccatcga gcgctcgaac atactcaact atccaggcaa aaccagcagc tcaaagagca
     6601 gcttcgcgat cgttaccggt ttgataatct aatcggcagc agcccggccg tcgtttccgt
     6661 ttacaaatcc atcgctcggg tggctcagac agacagcacg gttttgctgg agggtgaaag
     6721 cggaaccggt aaagaactca tcgcccgggc cattcatgcc aacagcgcac ggagtgccgg
     6781 ccccttcgtg acggtcgatg gcggagcatt gaccgaaact ctgctcgaat ccgaactgtt
     6841 cgggcacgaa cgaggcgcgt tcaccggcgc cgtgggcacc aaaaagggtc tattggagaa
     6901 agcccatctc ggcacatgtt tcctcgacga agtggccgat ctgtcgccgg cgctgcaagg
     6961 gaaattgctt cgtgtcattc aagagaaaga attccgacgc gtcggcagta ccatgaccac
     7021 aaccgtcgac gtacgcatta ttgccgcgtc gaaaaaagac ctcagttcac tcgtgcaggc
     7081 cggaacgttt cgtgaagacc tgtattatcg attgaatgtg gtgacgatac atatcccgcc
     7141 gttgcgggaa cgtatggaag acgtgccgct cttggcacag cattttgtgc agacctatgg
     7201 ttcagccaaa ccgaagccgg tcacgggaat gtccgctgaa gccatcgccg ttctgaccca
     7261 ctactggtgg ccgggaaacg tgcgggaact cgaacacgcc attgagcggg cgatagctct
     7321 tactccgcat cccattattt atcccgagga tcttccacat gccgtgcata cggccacggt
     7381 gcagacggcg gctcaagcac aaggatgggt caccttggag gaattagagc gagagcatat
     7441 cgtacgggtc ttgaagcacc atcaccacga tctgggtcgg tccgccgcga ttttagggat
     7501 tcaccgaaag acgttgttgc ggaaactacg gcaatacggt cttgccgatg ggcagcggac
     7561 aaggtatccg cctccggaca tcttgtctac gccggtccgg agtgggccgt ctcccgcaat
     7621 ctccaatcca cgggtattgg acccatcacg gtcttaggtc gtggactgtt ctttgccggc
     7681 tttcttcagc cgggtatgag ttggatgtac ttcgacttca tggatgatcg tgaccttgtc
     7741 agagggatct tcgggagtat cg
//
LOCUS       DATNDD010000324         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3368930, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000324 DATNDD010000000
VERSION     DATNDD010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368930"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VN647_07820"
     CDS             <1..1164
                     /locus_tag="VN647_07820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWG96974.1"
                     /translation="HPELIAAIASGIADSGPIPFVRFMELALYHPQFGYYMRSPEPPQ
                     ERIGRRGDFYTSSDIHPILGQALAKQAEQMYRLLGQPRPFTIVEMGPGKGLLAKQLLT
                     TCAHRYRSLFQHLRYVLVDRSPAMRELQRRNLAPWLDQPDLLAWVDDLANVAPQGLTG
                     LFFSNELVDSFPVHRVQVTAKGIEELWVDYRDGKFVECLMPLSSDTLVHLLRRVSADW
                     PEGYRTEINLRASEWMKQVAQPLDRGFVLTIDYGHTAQDLYRSDRKHGTFLCYFQQSI
                     NEDPFLRVGEQDMTAHVDFSSLAAIGEEQKLHMTGFTNQMSFLMGLGVEQMMAELEQD
                     SPQFKAAIHLLRPNGMGTTFKVLIQHKGIQQPELDGLQYKPFFDSVLTTQSAA"
     gene            1218..1589
                     /gene="ndhC"
                     /locus_tag="VN647_07825"
     CDS             1218..1589
                     /gene="ndhC"
                     /locus_tag="VN647_07825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWG96975.1"
                     /translation="MGYSAVPENYIPILIFIGVGIALGTLILLLGKFVRPNQPYRAKL
                     APYESGSPLFQDARVQFPMRYYIIAMLFVIFDIEIVFMYPWAIAFKNLGLVGLVEMVL
                     FIAILVVGFWYAWKKGALEWD"
     gene            1773..2252
                     /locus_tag="VN647_07830"
     CDS             1773..2252
                     /locus_tag="VN647_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWG96976.1"
                     /translation="MSLLERQFDANVLTTNLDAVVNWARKSALWPMTFGLACCAIEMI
                     ASVSSRYDLDRFGAGVFRASPRQSDLMIVAGTVSRKMAPVIRRIYDQMPEPRYVICMG
                     SCATSGNHYNSYAVVQGVDQIVPVDVYVPGCPPRPEALLDGLLKLQEKIQRERVFVK"
BASE COUNT          526 a          632 c          656 g          553 t
ORIGIN      
        1 catccggaac tcattgctgc cattgcctcc gggatcgccg attccggccc gattccgttt
       61 gttcgtttca tggagttggc tttgtatcac ccgcaattcg gttattacat gcgtagccct
      121 gaaccaccgc aagaacggat cggccggcgt ggtgattttt acacgagttc ggatatccat
      181 cctatccttg ggcaagctct ggccaaacaa gccgaacaga tgtaccggtt actcggccaa
      241 ccccgtccct ttaccatcgt tgaaatgggg cccggcaaag gcttgctggc gaagcaactt
      301 ctaacgacat gcgcgcatcg ctatcgttcg ttgtttcagc atctccggta tgtcctggta
      361 gaccgaagcc cggccatgcg tgaattacag cgcaggaacc tggctccctg gctggatcaa
      421 ccagacctgc tcgcatgggt ggacgacctt gcgaatgtgg cccctcaagg tctgaccggt
      481 ctgttcttca gcaatgaact cgtggactcg tttccggtcc accgcgttca ggtcacagcc
      541 aagggaatcg aggaactctg ggtggattat cgagacggaa agttcgttga atgtctgatg
      601 ccgctgtcgt cggacacact cgtccatctt cttcgccgtg tgagcgccga ttggccggaa
      661 ggttatcgaa ccgagatcaa cctccgagca tcggagtgga tgaagcaagt cgctcaacct
      721 ctggatcgtg ggttcgtgct caccatcgat tacggccaca cggcacagga cctctatcgg
      781 tccgaccgaa aacacggcac ctttctttgc tatttccaac aatccataaa cgaagacccg
      841 tttctgcgcg tgggcgagca ggacatgaca gcccatgtgg atttttcgag cttggcggca
      901 attggggaag agcaaaaact ccacatgacc ggcttcacga accagatgag ttttcttatg
      961 ggactcggcg tcgaacagat gatggcggaa ttggagcagg acagtccgca gttcaaagca
     1021 gcgattcatc tgctgcggcc gaacggaatg ggtacgacgt tcaaggtcct catccaacac
     1081 aagggaattc agcagcctga actcgacggt ttgcagtata agccgttctt tgattcggta
     1141 ctcacaacgc aatcagccgc gtgaggaatt tcgcgtaacg gcatcaccga cgttcggtaa
     1201 ccggttccga ggtctgtatg ggctactcag ccgttccgga aaactatatc ccgatcctca
     1261 tctttatcgg tgtgggcatc gcactcggaa cgctgattct tttgctcggg aagttcgtcc
     1321 gtcccaatca gccctatcgg gcaaagttgg ctccctatga gagcgggagc ccgctgtttc
     1381 aagacgcccg tgtacagttt ccaatgcggt actacatcat cgcgatgctc tttgtcattt
     1441 ttgatatcga gatcgtcttc atgtatccct gggctattgc attcaagaat cttgggctgg
     1501 ttggattagt agagatggtc ctgttcatcg ccatcctcgt ggtcgggttc tggtacgcat
     1561 ggaagaaagg ggcgttggag tgggattgaa gggcgatggg ctatccttcg tgctcgccca
     1621 ccgcgcacac aagatctcaa ttgaatagga gaagcgggtg gtgctccatg gggaagaacg
     1681 cgcctcggcg cgaccaggtt taggagggtg agaaatttgc gcagggaagg acatccccag
     1741 cacacttcgg tgagaacggg taggaaagaa acgtgagctt gttagaacga caatttgatg
     1801 ccaacgtcct gacgacgaat ttggacgcag tcgtcaactg ggccaggaag tcggcgttgt
     1861 ggcccatgac cttcgggcta gcctgctgcg ccattgaaat gatcgccagc gtctcctccc
     1921 gatatgacct ggaccgcttc ggcgccggcg tctttcgtgc atctcccaga caatccgatc
     1981 tcatgatcgt cgccggaacg gtctcgcgca agatggcgcc ggtcattcga cggatctacg
     2041 accaaatgcc tgagcctcga tatgtgatct gtatgggctc atgcgcaacc tcgggcaatc
     2101 actacaacag ctatgcggtc gtccagggtg tggatcagat cgtgccggtt gatgtctatg
     2161 tgcccggctg cccgccgaga ccggaagcct tgttggacgg actgttgaag ttacaggaga
     2221 agatccagcg agagagagtg ttcgtgaaat gagttcgtca aggatgcaaa gtggaaagtt
     2281 ctaaaattta agtctcctct tggaagacct ccaaacttgt aggctttcca atgctcacac
     2341 atgacgaccg aagaaaccat gcaatct
//
LOCUS       DATNDD010000325         2809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_376360, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000325 DATNDD010000000
VERSION     DATNDD010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376360"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..920
                     /locus_tag="VN647_07835"
     CDS             153..920
                     /locus_tag="VN647_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HWG96977.1"
                     /translation="MTLLELIGRWALIYMAEMGRMLIFVVTSFAWLARPPLRGMQIVK
                     QLHFIGYKSTFVVVLTAAFTGMVLALQGYYTLRKFGSEGLLGSAVALSMIRELGPVLA
                     ALMVTARAGSAITAEIGIMRITEQIDALDTMAVNPFQYLIAPKLVAGLIGVPLLVAIF
                     DVVGIYGGHLVGVDLLGVNAGSYWNSIEAAVEWKDVYGGILKSISFGLIVSWVCCYKG
                     FYTKMSAEGLGTATTQAVVLSSVLILIWDYFLTSLLL"
     gene            923..1681
                     /locus_tag="VN647_07840"
     CDS             923..1681
                     /locus_tag="VN647_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWG96978.1"
                     /translation="MLKLVDVTKTLGGQPVLQGIDLIVPEGKLTTIIGRSGEGKSVLL
                     KHMIGLLQPDSGQVWVDGIEISRLRGHALNEVRKRFAMLFQGAALFDSLTVFENVAFP
                     LRERLHMKGPEVTNRVEEKLDQVGLAGMGHKFPAELSGGMRKRAGLARALVMQPQIIL
                     FDEPTTGLDPLMAKSIHDLITSMQRKFGFTAVMVSHQIPEIFGISDYVAMLKHGKIAA
                     MAESAEFQRTTDPEIREFISVGGTVQLAKAVSGD"
     gene            1691..2137
                     /gene="mlaD"
                     /locus_tag="VN647_07845"
     CDS             1691..2137
                     /gene="mlaD"
                     /locus_tag="VN647_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HWG96979.1"
                     /translation="MMDKSRLELIVGLFVLVGIVCLGYLSIKLGKLELIGGDVYEVDA
                     LFNSATGLKAGATVEVAGVEVGRVKGIGLKEDRAMVRLSVHNGTKLYSDTIASIKTRG
                     IIGEKYLSLSPGGGGDPLKPGDTIRDTESGLDLEELVSQYVHGKVN"
     gene            2277..>2809
                     /locus_tag="VN647_07850"
     CDS             2277..>2809
                     /locus_tag="VN647_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWG96980.1"
                     /translation="MAICIGVVAPGYAGPPTDSMKATIDEVLRIVREKELKHPEKAEE
                     RRHLLEKVVALRFDYTEMSRRALGAPWNQLTDQQKQEFVDLFRTLLTNSYADKIETYS
                     GEGVQYLNERTEKDYAEVRTKVLSGKTEIPLDYRLINRADDWRVYDVVVDGVSLVNNY
                     RGQFTKILVPHPIRISS"
BASE COUNT          607 a          660 c          886 g          656 t
ORIGIN      
        1 gccgttttgg ccgctccttc atgtctcttg ggtcttggcc ggcttcgtcg acagagtctg
       61 gtcgcagcgg aggccttgac ggcttttttc cggaactatg cggaagcaat ggcgaccgga
      121 cgccggaaga aggaattctc gcagacttga ccatgacgtt actcgaactc ataggccgat
      181 gggcgcttat ctatatggcg gaaatgggac ggatgctgat cttcgtggtc acgtccttcg
      241 cctggttggc gcgtccgccg ctgcggggca tgcaaatcgt caagcaactc cacttcatcg
      301 gctacaagtc gacgtttgtt gtcgtgctga cggcggcgtt caccgggatg gtgcttgcgc
      361 tacagggcta ctatacgctg cgaaagttcg ggtctgaagg gctgttgggc tccgcagtgg
      421 cgctcagcat gattcgtgag ttgggccccg tcttggccgc gctcatggtg acggcgcgtg
      481 ccggttccgc gataacagcg gagatcggta tcatgcggat cacggaacag atcgatgcgc
      541 tcgataccat ggccgtcaat ccgttccaat atctgattgc gccgaagctc gtggctggtt
      601 tgatcggcgt tcccctgctg gtcgcgatct tcgacgtggt aggaatctac ggcgggcacc
      661 ttgtcggcgt ggacctactc ggcgtcaacg ccggttcgta ttggaattcc atcgaggcag
      721 cggtcgagtg gaaggacgtc tatggcggca ttctcaaatc cattagtttt ggtctgattg
      781 tgagctgggt ctgttgctat aagggctttt ataccaagat gagtgccgaa gggctcggca
      841 ccgccacgac acaggcggtc gtattgtcat cggtcttgat tcttatctgg gactattttt
      901 tgacgtcatt gttgttgtaa tcatgctgaa acttgtggat gtgacgaaga cgttgggagg
      961 tcagccggtt ttgcagggca ttgatctcat cgtccccgaa gggaaactca cgacaatcat
     1021 cggtcgaagc ggtgaaggca agagcgtgct gctgaagcat atgatcgggc tcctccaacc
     1081 tgattccgga caagtgtggg tcgatggaat cgagatttct cggcttcgcg gccatgcact
     1141 caacgaagtg cgcaaacggt tcgcgatgtt gtttcaaggg gcggcgctgt tcgattcact
     1201 gactgtcttt gaaaatgtgg cgtttccgct cagagaaaga ctccacatga aaggcccgga
     1261 ggtgaccaac cgtgtcgagg agaagttgga tcaagtgggt ctggccggga tgggacacaa
     1321 gttccccgct gaactcagcg ggggcatgcg aaaacgcgcc ggcttggcac gtgcattggt
     1381 aatgcaaccg caaattattc tcttcgacga gcccacgacg ggtctcgatc cgctcatggc
     1441 caaatcgatt cacgatctta ttacgagcat gcagcggaag ttcgggttta ccgctgtgat
     1501 ggtcagtcat cagattccgg agatcttcgg tatttcggat tacgtggcaa tgttgaagca
     1561 cgggaagatc gcggcaatgg cggagtcggc cgaatttcaa cggacgaccg atcctgaaat
     1621 cagggaattt atctcggtcg gtgggacggt gcagctggcc aaggcggttt ccggagacta
     1681 gaggagtgcc atgatggaca aaagcagatt ggagctgatc gtcggtctgt tcgtgctggt
     1741 cggcatcgtc tgtttgggct atctctcgat caaactcggc aagttggagc tgatcggtgg
     1801 agatgtctac gaagtcgatg ctctgttcaa ttcagccaca gggctgaaag ccggagcgac
     1861 tgtggaagtc gccggtgtcg aggtgggcag agtcaagggc atcggcttga aagaagatcg
     1921 ggcaatggtg aggctgtcgg ttcacaatgg gacgaaactc tattcggata ccatcgcgtc
     1981 gatcaagacc cgagggatca tcggagaaaa atacctttcg ctctcgccgg gtggaggggg
     2041 agatcctctg aaaccgggcg acacgattcg tgataccgaa tcaggccttg atcttgagga
     2101 gttagtgagt cagtacgtcc atgggaaggt caactgaccg gatctatctc ttaggtgtgg
     2161 cgggaacgac taatatattg gtgtaggaga gaggcgcgat gatagcgatt aggaatgctt
     2221 ggattaggcg gagagggggc agttcgtggt acacggtgct cttaacggcg ctcataatgg
     2281 ccatctgtat cggagtagtg gcacctggat acgcgggccc tccaaccgac tccatgaagg
     2341 cgacgattga tgaagtgctc cgcattgtac gggaaaagga actcaagcat ccggagaaag
     2401 ccgaagagcg tcgacacttg cttgaaaaag tggtggcgct gcggttcgac tatacggaaa
     2461 tgtccagacg ggcgcttggg gctccctgga accagctgac cgatcagcag aaacaggaat
     2521 ttgtcgatct gtttcggacg ttgctgacaa attcgtatgc ggacaagatt gagacctatt
     2581 ccggcgaagg cgtgcagtat ttaaacgagc gaaccgagaa ggactacgcg gaagtcagga
     2641 ccaaggtgct ttcaggaaag acggagatcc cgctcgatta tcgtttgatc aacagggcgg
     2701 atgattggcg ggtctatgac gtcgtcgtgg acggggtcag cctggtgaac aactatcggg
     2761 ggcagtttac gaagattctt gtgcctcatc ctattcggat ctcgtcgat
//
LOCUS       DATNDD010000326         4023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_485613, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000326 DATNDD010000000
VERSION     DATNDD010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485613"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /gene="rpoB"
                     /locus_tag="VN647_07855"
     CDS             complement(<1..2229)
                     /gene="rpoB"
                     /locus_tag="VN647_07855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWG96981.1"
                     /translation="MSETTLQEFVERKDFSRIRTNIDIPDLIEIQKRSYEEFLQFEVE
                     SERRKDHGLQAALASVFPIPDYNNTAVLEFSSYTLGTPKYDERECLEQGMTFAVPLKL
                     RVRLVVLDKEDKGPRKKVLDVREQEVYVGELPLMTERGTFIVNGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGFSSDDLLKMYYPVEEIRVSKGKMFRKLDPEIHHGLRCSAEVTDKGGKEPLVREGAR
                     LTKGLIAKLKASGVKEIPLLPTELVGRAILTELVDSKKNRLAEKNQRLTAEMVEQIAE
                     SDVEEFKVIYLDTATATPVILDTLEMEKIGSKEEAMVEIYRRLRPGETPSVETARALF
                     DNLFLNSKRYDLSPVGRLKLNKKLGLDLPLEQRTLTAQDIVEVIRYLVNLKIGKGEID
                     DIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVAA
                     VKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRIC
                     PIETPEGPNIGLITSLATYARINQFGFIEAPYRKVVKGRVTDEIEFLSAIEGDKYIIA
                     QANSKLDGAGKLVSETVSCRHGGDFVLAAPDKIEYMDVSPKQVVSVATALVPFLEHDD
                     ANRALMGSNMQRQAVPLVTSEAPLVGTGMEAVVARDSGYVIQARRAGVVESVDATRIV
                     VRA"
     gene            complement(2355..2756)
                     /gene="rplL"
                     /locus_tag="VN647_07860"
     CDS             complement(2355..2756)
                     /gene="rplL"
                     /locus_tag="VN647_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWG96982.1"
                     /translation="MPATEGKLSQEELIKAIESMSVLDLAELVKGLEARFGVTAAAPV
                     AVAAAPAAGGGAAAPAEEKTAFDVVLASAPADKKIQIIKVVRELTSLGLKEAKDLVEG
                     APKPVKTGVAKEEADTMKKKLEESGAKVEIK"
     gene            complement(2784..3290)
                     /gene="rplJ"
                     /locus_tag="VN647_07865"
                     /pseudo
     CDS             complement(2784..3290)
                     /gene="rplJ"
                     /locus_tag="VN647_07865"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(3377..4015)
                     /gene="rplA"
                     /locus_tag="VN647_07870"
     CDS             complement(3377..4015)
                     /gene="rplA"
                     /locus_tag="VN647_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWG96983.1"
                     /translation="MREAVETVKRSAYAKFDESVDLALRLGIDPKRSDQLVRGTASLP
                     HGTGKKVRVLVFAKGEKEQEARQAGADYVGADDLMEKIKGGWMDFDCAISTPDLMASV
                     GKLGKVLGPRGLMPNPKTGTVTFEVGKAVADIRKGRVEFKVEKAGIVHVPVGKVSFDP
                     AKLYDNASAILESVIKAKPASCKGRYLKSATIASTMGPGVKLDTIALTKQWS"
BASE COUNT          821 a         1305 c          963 g          934 t
ORIGIN      
        1 ggcccgcacc acgatgcggg tggcatcgac gctttccacg accccggctc gacgagcctg
       61 gatgacatac ccggaatctc gcgccaccac cgcttccatc cctgtcccaa ccagaggggc
      121 ttccgacgtc accaagggaa ccgcctggcg ctgcatgttg gaacccatca gcgcacggtt
      181 ggcatcgtcg tgttccaaga acggcacgag cgcggtcgcg acacttacta cttgcttcgg
      241 tgacacgtcc atgtactcaa tcttatccgg agcagccagg acaaaatctc caccgtggcg
      301 acaggagacg gtttccgata ccaacttgcc cgcgccatcc aattttgagt tggcctgagc
      361 gatgatgtat ttgtcgccct cgatcgccga gagaaactct atttcatccg tcacacgtcc
      421 cttgacgact ttccgatatg gtgcctcaat gaatccgaac tgattgattc gtgcgtaggt
      481 cgccagcgaa gtaatcaatc cgatattggg accttccggc gtctcgatcg ggcaaatccg
      541 actgtagtga gacggatgaa cgtctcgaac ttcgaacccg gccctctctc tcgtgagtcc
      601 gcccggacca agagccgaca ggcgccgttt atgcgtaatt tcagccagag ggttcgtttg
      661 gtccatgaat tgagacagct gactgctgct gaaaaactcc ttgacggctg cgacaaccgg
      721 cttggcattg atcaagtcat gcggcagcac cgtttccata tcaagaagat tcatgcgctc
      781 cttgatgctt cgctccatcc gcaccaaccc cagccggaat tgattttcaa ggagctcacc
      841 gacggatcgc acgcggcgat tacccaagtg atcaatatcg tcgatttccc ctttgccgat
      901 cttcagattg accagatagc ggatgacttc cacgatgtcc tgagctgtca gggtgcgctg
      961 ctcgagcggc aagtccaaac caagcttttt attcagcttg agccggccga ccggagacaa
     1021 atcgtagcgc ttggaattca gaaagagatt gtcgaacaat gccctcgccg tttcgactga
     1081 cggcgtctca ccgggacgga ggcgacggta gatttcgacc atcgcttcct ccttcgaccc
     1141 gattttctcc atctccaagg tgtcaagaat taccggcgtc gcggtcgccg tatccaggta
     1201 aatgaccttg aattcttcca catcgctttc agcgatttgt tcgaccatct cggccgtcaa
     1261 tcgttggttt ttctccgcta acctgttctt cttcgaatcg accaattccg tgagaatggc
     1321 gcggcccacc aattccgtgg gcagtaaggg aatctccttc actcccgagg ccttcagctt
     1381 ggcaatcaag cctttcgtca gcctcgcccc ctcccgcacc agcggttctt tgccgccctt
     1441 gtccgtgacc tcggcggaac accgaagtcc gtggtggatc tcgggatcca gcttgcggaa
     1501 catcttcccc ttcgacacgc gaatttcttc cacggggtag tacatcttca gcaagtcgtc
     1561 gctcgagaaa ccgaaggcct tcaacagaat ggtggccggc attttccgac ggcgatcgat
     1621 ccgcacatat agaatatctc tggcatcgaa ctcgaaatcg agccatgagc cccgataggg
     1681 aataattctg gccgagtaca acaccttgcc gctcgcatgg gtccgacctt tgtcatgtgt
     1741 gaacgacgca cccggtgacc ggtgaagctg gctgacgacg actcgctcgg tcccgttgac
     1801 gataaaagtc ccccgctcgg tcatgagcgg caattcaccg acatagacct cctgctcacg
     1861 cacatcgagc actttcttgc gaggcccctt atcctcttta tcgagcacca ccaggcgaac
     1921 acgcagcttc aatggaaccg cgaaggtcat accctgctca agacattcgc gctcgtcgta
     1981 ctttggcgtc cctaacgtat agctcgaaaa ttcgagcacg gccgtgttgt tatagtccgg
     2041 aatcggaaac acgctggcca acgccgcctg caatccatga tcctttcgac gctccgattc
     2101 gacctcgaac tgcaaaaact cttcgtagga gcgcttctgg atctcgatca gatctggaat
     2161 atcgatgttg gttcgaatgc gggaaaaatc tttccgctcg acgaattctt gaagcgtcgt
     2221 ttcggacatt cctactcctc cacgctggtt ggcggtgaac agccgtcacc cgtcacgaca
     2281 gattcccgaa ataccgataa acctcggcct tcagcgaata ccgaaagagc cgcgacgatg
     2341 acgaactcgc ttccttactt gatttcgacc ttggcaccgc tttcttcgag tttcttcttc
     2401 atcgtatcag cctcttcctt ggccaccccg gtcttgaccg gtttcggcgc gccctccacg
     2461 aggtccttgg cttccttcag gccgagactc gtcagctccc gcaccacctt gatgatctgg
     2521 attttcttgt cggccggcgc cgacgcgaga acgacgtcga atgccgtctt ctcttcggcc
     2581 ggagcagctg cgccgccgcc agccgccgga gccgcagcca ccgcaaccgg cgccgccgcc
     2641 gtcacgccga accgcgcctc aagccccttc accaactcgg ccagatcaag cacgctcatg
     2701 ctttcgatcg ccttgatcaa ttcctcctgc gataattttc cttccgtagc cggcatgtcc
     2761 ccttctcctt tccgtttatc ttgaatggct gcaatgactc gcacaaactt cgacacgacc
     2821 gcgttcaacg tatagacaac gccacgaatc ggcccctgca tggccgaaag caacatggca
     2881 acgagcactt cctttttcgg caacctcgca acggcggcca gttcagccgg ctggaggatt
     2941 ttgccttcca gcaccccggc cgtcatccga atcttctctt cacgcttctc cgcgccgata
     3001 aattctttga gcacctttgt cggcaacacc ggatcgtcat agccgatgac taccccggtc
     3061 ggccccttga gatgcacttt gagtccggcc aatatcgtcc cctcggcggc acgcgccgcg
     3121 agcgtattct tcacaattcg atattctgcc tttgccccgc ggagctgttt gcgtaactca
     3181 gtgatctggt tgaccggaag gccgacgcat tccgtcaaga tagccagccg cgcacgaccg
     3241 aatttctcgg tcaactccgc cactgccgta accttctctt ccttcttcat cgctccctct
     3301 ctcgtcgcta atcgttatcc gttcaacgtc acccgtccgg cgagttgcga tgaacgattc
     3361 acgtcgaatg cgtttttcag ctccactgtt tcgtcagcgc aatggtatcc agtttcacac
     3421 cgggtcccat cgtacttgca atcgtggcac tcttgagata acgccccttg catgaggcgg
     3481 gtttcgcctt gatgacggat tcaaggatgg ccgatgcatt atcatacagc ttcgcgggat
     3541 cgaacgagac ttttcccacc ggcacatgga cgatcccggc cttctcgacc ttgaactcga
     3601 cacgcccctt tcgtatatcg gcaacggcct taccgacttc gaaggtgact gtgccggtct
     3661 taggattcgg catcagcccc cgaggcccga gtaccttgcc cagttttccg accgaggcca
     3721 taaggtccgg cgtggaaatc gcgcaatcga aatccatcca tccacccttg attttttcca
     3781 tcaagtcatc agccccgaca tagtcggcac ccgcttgccg cgcctcctgc tccttttcac
     3841 ctttggcaaa caccaggaca cgaacctttt tcccggttcc gtgtggaagc gaagccgtcc
     3901 ctcgaaccag ttgatccgag cgcttcggat cgattcccag cctgagcgca agatcgaccg
     3961 attcgtcaaa cttcgcatag gcagaccgct tcacggtttc gaccgcctca cgcaacccat
     4021 aga
//
LOCUS       DATNDD010000327         2857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3298592, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000327 DATNDD010000000
VERSION     DATNDD010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298592"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..728)
                     /locus_tag="VN647_07875"
     CDS             complement(345..728)
                     /locus_tag="VN647_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWG96984.1"
                     /translation="MDQETRHTLAVTSRQNAGVTFVKMQGSLSATTAEQGIQEMKKIV
                     DSGAKKVVINLADVDYISSGGIRVLILACKQLNNVQGEMKIAAAKGMVKEALETGGFN
                     LLSRVYGENNQLCNTEEEAVAAFKV"
     gene            complement(922..>2857)
                     /locus_tag="VN647_07880"
     CDS             complement(922..>2857)
                     /locus_tag="VN647_07880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HWG96985.1"
                     /translation="VQLGDSFESIIRRIAERGDIPAAIGNVESWIQKRLDMHHNPQGL
                     YIQYRSSGEWIQIHERRTHDGGIVFIATDITALKRAEQAAEDAKTRLADSLAVVEAAK
                     ARMQEELNVGRDIQQSMLPRVFPAFPDRKELELYAVLEPALEIGGDLYDFFLLDDHRL
                     CFVIGDVSGNGVPAAMFMAMTKIMVKTRASSDPSPASIVTHVNDALSAENDSCMFVTL
                     YLGILNLRDGTLLATNAGHNPPLLKRRDGQFDWLTAQDGPLVGPMPGIAFKESLIQLR
                     PGDELFLYTDGVTEADNRRRELFGKDRLKAVLDRSRAVSLVDRIGDVMKAVKTFAGDA
                     PQADDITILGIRYLGVAPSDVSVSVFRQTMLNQLAAIPMVQTAFERYVAQWERAKPLI
                     PTFNMALDDLLNNVVQYAFPNDPTEHTIEVEGEVRDECVVLTITDDGIPFNPLTVATP
                     DLSLLLHEREIGGLGIHLVRSMFDEVTYHRNVGRNVLTVKKKFVSEPPASLKRSDQTG
                     IRVPEGLWKSPPPGQDSTSMTMSVTSHRNGDVMIVTPQNRFDTNSAPEVERILTEHIE
                     RGKRRIVMDLSRISYISSIGLRVILKAVITMTRAGGRVVLSGGNDHVGTVLQLSGTLM
                     MSLHASTLEDAVAKVRKTGS"
BASE COUNT          601 a          832 c          759 g          665 t
ORIGIN      
        1 caaaaagggc gcagaggcca tcctgcagct tcgcctcccg gatcaccgtc tcaagccgat
       61 cggtcagatc gacgatctct ttcgcggcgg ttgttttgat acggaggact tggcccatag
      121 gaaatggatc tcaggaaaga ataaaacatg gtagagaaga cgggaaactc agtctactag
      181 gagaaccccc gatcctttac cgccgactca gtgtcagggc ttgcgagtga gcatgcaaat
      241 aaccagagct cctgaaagcg atgtcttgcg tgccggtgtc aacagatttt gaggcggaga
      301 ttctttttcg gcaccgaggc cgaatgagag gtggatcagg cgctctagac tttaaaagcg
      361 gccacagcct cttcttccgt attgcacaat tgattatttt ctccatatac gcgggacagc
      421 agattaaatc caccggtttc aagagcttct ttgaccatcc ccttcgctgc ggcaatcttc
      481 atctcccctt gaacgttatt aagctgcttg caggccagaa tcaggacgcg gatgccgccg
      541 ctgctgatgt agtccacatc agccaggttg atgacgacct ttttcgcgcc tgagtccacg
      601 atttttttca tctcttggat tccttgttcg gccgtggtgg cggaaaggct accctgcatc
      661 ttgacgaagg tcactccggc gttctggcga gaggtcaccg ctaacgtgtg tcgggtctct
      721 tggtccatgg ttacactcct tttttcttcg cttcttcctc aatcatctcg ttcacaacgg
      781 ctgtcctctt cgcaccgggt tcgccgctcc caccgtgcgc cccatcatac catttataca
      841 ttccctcctt ccatccggac gatcctaaac cgggcgtcaa caagtcaccg aggggctcgc
      901 acatcggtca atcgaaaggt gtcatgagcc tgtctttcgg accttcgcga cggcgtcttc
      961 cagcgtggag gcatggaggc tcatcatcaa ggtcccactc aattggagca ctgttccgac
     1021 gtggtcgtta cctccactga gtacgactct tccacctgct cgggtcatgg ttatcacggc
     1081 tttcaagatc acccgcagtc caatggatga aatgtacgaa attcgagaga gatccattac
     1141 gatccgccgt ttcccacgct caatgtgctc cgtcaggatg cgctcgacct ccggcgcgct
     1201 gttcgtgtcg aaacggttct gcggggtgac gatcatcaca tcaccgtttc gatgtgatgt
     1261 gacgctcatg gtcatgctcg ttgagtcttg gcctggtggt ggcgacttcc ataggccttc
     1321 gggcaccctg atcccggtct gatcggaacg tttcagagag gcgggcggct cggagacgaa
     1381 cttctttttc accgtcaaga cgttgcgccc cacgttgcga tggtacgtca cctcgtcaaa
     1441 catggagcgc accaagtgaa ttcccaaacc gccgatctcg cgttcgtgca ggagcagcga
     1501 cagatccggg gtggcgaccg ttaaggggtt gaagggaatc ccgtcatccg taatggtcaa
     1561 gaccacgcac tcatcgcgca cttcgccttc cacctcaatg gtgtgctcgg tcggatcatt
     1621 tgggaaggca tactgcacga cattgttgag cagatcatcc agcgccatgt tgaatgtggg
     1681 gatcaacggc ttggctcgtt cccattgcgc cacatatcgc tcaaatgccg tctgtaccat
     1741 gggaatggcc gccaattggt tgagcatggt ctgacgaaac acgctcaccg acacatcgga
     1801 cggggcgacg ccgagatacc gtatccccaa tatggtgatg tcgtccgcct gcggtgcgtc
     1861 accggcgaag gtcttcaccg ccttcatgac atccccgatg cgatcgacca acgagacggc
     1921 tctggatcgg tccagtaccg ctttcaaccg gtctttgccg aacaattcgc gccgcctatt
     1981 gtctgcttcg gtgactccat cggtgtacaa gaagagctca tctcccggtc tcaattggat
     2041 gagactctcc ttgaaggcaa tgccgggcat cggaccgacg aggggcccgt cttgagccgt
     2101 caaccagtca aactgtccat ctcgccgctt caacagagga ggattatggc cggcattggt
     2161 cgcgaggagc gtgccgtcgc ggagattcaa gatgcccaga tacagggtca cgaacataca
     2221 ggaatcgttc tccgcgctga gcgcatcatt cacatgggtg acgatactgg cgggcgaggg
     2281 atccgacgac gcccgagtct tcaccataat tttggtcatg gccatgaaca tcgcagcagg
     2341 gaccccgttg cccgacacat cgccgatgac gaaacagagc cgatggtcat cgagcaggaa
     2401 gaaatcgtac aaatcgcctc cgatttccag ggccggttca aggactgcat agagctccag
     2461 ctccttccga tccggaaaag ccgggaatac ccgtgggagc atactttgtt gaatatctct
     2521 gccgacattc agttcttcct gcatgcgggc tttcgcggcc tccactaccg ccaatgaatc
     2581 cgccaaccgc gtcttggcat cttcggcagc ctgttccgca cgtttcagcg ccgtaatgtc
     2641 ggtagcaatg aagacaatgc ctccgtcgtg cgtcctccgc tcgtgaatct ggatccactc
     2701 gccgctggaa cggtattgaa tgtagagacc ttgagggttg tggtgcatgt cgaggcgctt
     2761 ctgtatccaa gattcgacat tcccgatcgc agcgggaatg tcaccccgtt cggcaatacg
     2821 gcgaatgatc gattcgaacg aatcgccgag ctgtacc
//
LOCUS       DATNDD010000328         2644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1285616, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000328 DATNDD010000000
VERSION     DATNDD010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285616"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VN647_07885"
     CDS             complement(<1..255)
                     /locus_tag="VN647_07885"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HWG96986.1"
                     /translation="MHLEDYAFIGDTHTGALVGRNGSIDWLCLPRFDSNACFAALLGN
                     EQNGRWMIAPADQEHRVTRRYRPDTLILETDFTTKDGTVRV"
     gene            complement(454..951)
                     /locus_tag="VN647_07890"
     CDS             complement(454..951)
                     /locus_tag="VN647_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96987.1"
                     /translation="MNALWRKFIRIVVSVPHYAVIALLWPLAWGGCLLSVWLAYRTAE
                     FIYAHVFQITHEAFRDLLSALLHSIELLLLVPLPGIVGIVAYRTLRLYVNPIAKQRPS
                     IETELEMAKRLVLGTLVSVAGTRMLIQLLEEKTELNLYISGGLLIVSLTFYTVFALGK
                     GRGGH"
     gene            1003..1221
                     /locus_tag="VN647_07895"
     CDS             1003..1221
                     /locus_tag="VN647_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96988.1"
                     /translation="MHRHLSARATLKVEMRFGLAYAQVADFQMMNEIGQERPDDSEAQ
                     PNASSINPSMVAIRYRMVPEAFGWSATG"
     gene            complement(1251..2291)
                     /locus_tag="VN647_07900"
     CDS             complement(1251..2291)
                     /locus_tag="VN647_07900"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWG96989.1"
                     /translation="MRQNERRLEVVVVTGASAGVGRAIVREFAKHGASIGLLARNRDG
                     LEGARREVEEAGCRAVAIPTDMSDPKQVEDAADTVTKELGPIDVWVNDAMVSVISPAV
                     EMTPEDYQRVTNVSYLGYVYGTLAAVRRMKERNKGKIIQVGSALAYRSIPLQSAYCGA
                     KHAIQGFTDSLRSELIHDGSNIDITTLQLPAVNTPQFTWIKSRMPHHPQPVPPIYQPE
                     VIARAAYWAAHHRRRELWIGFSAVKAIIGQKLMPGFADHYLAWMGYHSQQTDQPIDPN
                     RPNNLYEPLSGDFGAHGEFDGRSRPFSLQSWLNMHRNWMSLVGLGVVAGYVCYAKAQS
                     KQARQWLGYQRH"
     gene            complement(2453..>2644)
                     /locus_tag="VN647_07905"
     CDS             complement(2453..>2644)
                     /locus_tag="VN647_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96990.1"
                     /translation="KKFRESNGWKAGVDGSVVLVNLGAEGSVDTKKFNDPIVGFVFGQ
                     KGLMYDLSLEGSKFTKIDK"
BASE COUNT          579 a          742 c          721 g          602 t
ORIGIN      
        1 gacccgtacg gttccatctt tcgtcgtgaa gtccgtctcc agtatgaggg tatccggccg
       61 gtagcgtcgc gtgacacgat gttcttgatc ggcaggcgcg atcatccaac gtccgttctg
      121 ttcattgccg agcaaggctg caaaacaggc gttggaatcg aagcgcggta ggcataacca
      181 atcgatggat ccgttacggc ctactaacgc tcccgtatgt gtatcgccga tgaaggcgta
      241 gtcttcgaga tgcatcaatt tcaccagccg gcaagttggg tgatggaaat gtgtctcgaa
      301 aaggtggtgc aagcgaaatg ccatgagaga ggacctgaaa aatattcatg gttatctaat
      361 ctcgattggg acaatttgac atcatcatga cagttgttcc cgagttcatg tgttttcgag
      421 tgttcgtcta gtagcgatac catgtatgtc acgttaatgt cctccccgtc cctttccgag
      481 tgcgaacacc gtatagaacg tcagcgacac gatcaagaga ccgccgctga tgtacagatt
      541 cagttccgtc ttttcctcga gcagttggat aagcatccgt gtaccggcca ctgagaccag
      601 cgtgccgagc accaaccgct tcgccatttc gagttctgtc tcgatcgacg gccgttgctt
      661 ggcaatcgga ttgacgtaga ggcggagagt gcgataggcg acgatgccga cgatgccggg
      721 gagggggacc agcaatagga gttcgattga atgcaacagg gcggacagaa ggtctcgaaa
      781 cgcttcatga gtgatttgaa aaacgtgggc gtaaatgaat tcggcggttc ggtacgccag
      841 ccagacggac aacagacacc ctccccacgc caagggccag agcagcgcta tgaccgcata
      901 gtgcggcacc gagaccacga tgcggatgaa tttccgccac agtgcgttca cctcgcgtgc
      961 tcgaatcctt caacggccac ttccagatga ccgttgtaga acgtgcaccg ccatctgtcc
     1021 gctcgggcca cgctcaaagt tgagatgcga ttcggcctgg cctacgcgca ggtggcggac
     1081 ttccagatga tgaatgaaat cgggcaagag cggcctgacg attctgaagc gcagccgaac
     1141 gcgtccagca tcaatccgag catggtggcg atcagatacc gcatggtacc cgaggccttc
     1201 ggatggtcgg cgaccggata acggacgaga gcagttgatc ggtcttcgcc tcaatgacgt
     1261 tggtatccga gccattgcct ggcctgcttg gattgtgctt tcgcatagca cacgtatccg
     1321 gcaaccacgc caagaccgac caggctcatc caatttctat gcatgttcag ccaagactgg
     1381 aggctaaacg ggcgcgatct gccgtcgaac tcgccgtgcg cgccgaaatc tcccgacaac
     1441 ggctcataca agttattcgg acggttggga tcgatcggtt gatcggtctg ttgtgagtga
     1501 tagcccatcc aggccagata gtgatcggca aatccaggca tgagtttttg gccgataatc
     1561 gccttgaccg ctgaaaaccc gatccagagc tcacggcgtc ggtgatgtgc ggcccaatag
     1621 gcggccctgg cgatgacctc cggctgataa atcggtggaa ccggttgtgg atggtgaggc
     1681 attcgggatt tgatccaggt gaattgcggc gtgttcacgg ccggcaattg cagcgtcgta
     1741 atatcgatgt tgctgccatc gtgaatcagc tcggatcgta gcgaatcggt aaatccctga
     1801 atcgcatgtt tggcgccaca ataagctgat tgcaagggga ttgatcggta agcgagggcc
     1861 gagccgactt ggatgatctt gcctttgttg cgttccttca ttcggcggac cgccgccagc
     1921 gtgccataga catacccaag ataggagaca ttggtgaccc gctgatagtc ttcgggcgtc
     1981 atctcaacgg ccggtgaaat cactgagacc atggcgtcgt tcacccacac gtcaatcggc
     2041 ccaagttcct tcgtcacggt atcggccgca tcctcaacct gcttgggatc cgacatatct
     2101 gtgggaatag ctacagcgcg acaaccggcc tcttcgacct cccgcctggc accctccaat
     2161 ccatcgcggt ttctggcgag caaaccgatg gacgctccat gtttcgcgaa ctcacgcacg
     2221 atggcgcgtc cgacgccggc tgaggctccc gtcacgacga ccacttctaa gcgcctctca
     2281 ttctgtcgca tcccacgctt cctcccgtgg tccatcacac gacttcaaga tgtctgcgac
     2341 ggtttccaac atatttcctc ctgtctgtat gttttccgca tgcacgatgt cgtcggtgtt
     2401 gctcatcatg ctgcagcacc cacgatgaag aggcagcgaa tgctcggttt ctttacttat
     2461 ctattttggt aaactttgag ccttccaacg acaagtcgta catcagtccc ttctggccga
     2521 agacgaagcc gacaatcggg tcgttgaatt ttttcgtgtc gactgaacct tctgctccga
     2581 gattcaccag cacgacagag ccgtccaccc cagccttcca gccgttactc tcgcgaaatt
     2641 tctt
//
LOCUS       DATNDD010000329         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2600888, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000329 DATNDD010000000
VERSION     DATNDD010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600888"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VN647_07910"
     CDS             complement(<1..351)
                     /locus_tag="VN647_07910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG96991.1"
                     /translation="MDPNKIERVYTSYAGFYDKVFGKVFHEGRESAIRNLNVQPDERI
                     LEVGVGTGLALPMYPRHCRIVGIDLSEGMLAKAKEKAEAHSLDHVQLHRMDAGAMDFM
                     DDSFDTVVAAYVVTA"
     gene            635..1252
                     /locus_tag="VN647_07915"
     CDS             635..1252
                     /locus_tag="VN647_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96992.1"
                     /translation="MILAGFFAGILFIGLIVGDWLYFIRLTPDAIRYGCRVANNPDQW
                     PGTALTTLGDRFTPEGVLMLPHGVAWYYPQLSQIAIRPQYRLFSRRFRTAWPVKGLIH
                     LSPHDQSLNTLCVKRMPWSSALMTLLWFALVLIGSLTFMVQFVMDGGFASLGGTLTGV
                     AVIALAALVLAFGLVTVALAYRLEDSRLMKVYGELRDAVALTTSA"
     gene            complement(1249..1617)
                     /locus_tag="VN647_07920"
     CDS             complement(1249..1617)
                     /locus_tag="VN647_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96993.1"
                     /translation="MSEDSLTTPDPLQTLKAEFRHHLETFYAGLQLAPPYESVEKAIR
                     TLTTMIHGLPIQEQTRILADPGLRWRQFRLAFDASGLAKKHRGIIVGLARNRSTLSLP
                     TEYDHFLNLFSDRKEPHGLG"
     gene            1680..2165
                     /locus_tag="VN647_07925"
     CDS             1680..2165
                     /locus_tag="VN647_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96994.1"
                     /translation="MNRLFNGLCALMGVLILTACSSYRHHHGMMSDGKSEAYWQKGQQ
                     DMEGLINRTVKEPDRAKQVNVIVGEIIKELKTSREQERAYHRDLYTLNASYTAMPDEF
                     SKILNEANIQRMQSSAKVLSLRFKMKDLMTADEWKALTDKMLSYSSRYQHGESGAKTG
                     Y"
     gene            complement(2224..3270)
                     /locus_tag="VN647_07930"
     CDS             complement(2224..3270)
                     /locus_tag="VN647_07930"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96995.1"
                     /translation="MMLLRIIITLGLFALLSFSLGCQKESESIVSIALHPTNTNILYV
                     ATNDAVYKSRDGGGTWERFPSFSARRVTTLAIDPQLPAAIYAGTMGDAVYKSPDGGQH
                     WLPHNVGLKEHVSFVNQFVFHPALSEKIYIATTVGAFLTKDGGREWEERMNGMKEVHI
                     VTSIAINPKDPTILYGGTTGGMYRSDDGAMSWKRVNNGLIPDSELMASMALGVNAIEI
                     DRATPDTVYAGTTKGLFRTTNKGEQWERIGQSLTDPFISSILLHPTEPLQLYVGGPKG
                     VWKSSDGGKSWQAVNQGIVTLNIRALALNPKNPQVLYAGTNGSGLYRSTDAGATWIGV
                     PLKAAPAAVGSGQG"
     gene            3476..3700
                     /locus_tag="VN647_07935"
     CDS             3476..3700
                     /locus_tag="VN647_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWG96996.1"
                     /translation="MAELVERITVNPEQCGGRPCIRGMRIRVVDVLDLLAAGLSQQQV
                     LEELPDLETEDISACLKFASSRLNHPILAA"
     gene            3697..4041
                     /locus_tag="VN647_07940"
     CDS             3697..4041
                     /locus_tag="VN647_07940"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWG96997.1"
                     /translation="MKIWVDAQLSPVIAKWLSSTYRVDTVAVRGLRLRDARDRDIFLS
                     AREAHATILTKDRDMVDLVIRLGSPPQILWVTCGNTSNAKLKTILTKAWPVAENLLKA
                     GEKVVEISDTTG"
     gene            4077..4289
                     /locus_tag="VN647_07945"
     CDS             4077..4289
                     /locus_tag="VN647_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG96998.1"
                     /translation="MSKKLTDKALANFEAKRDVWQEVLDGVREIKAGGEKQTKLKLSR
                     AYFAHSSCNCTLTPIRSIAFLLHFES"
     gene            4296..4514
                     /locus_tag="VN647_07950"
     CDS             4296..4514
                     /locus_tag="VN647_07950"
                     /inference="COORDINATES: protein motif:HMM:NF025021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWG96999.1"
                     /translation="MAPEKLFGPENMSVLEALKRKDRSLALERLCKGAVLNDKDEYGN
                     TALHYAAAFGYMDVIEKILEARSDLAVC"
BASE COUNT         1107 a         1209 c         1236 g         1088 t
ORIGIN      
        1 cgccgtcacc acataggcgg caaccactgt atcgaaactg tcatccatga agtccatggc
       61 acctgcgtcc atgcgatgaa gctggacatg atcaaggctg tgggcttcgg ccttttcctt
      121 agcttttgcc agcatgcctt cggacagatc gatcccgaca atccggcaat ggcgcggata
      181 catcggaagg gcgagacccg ttcccacccc cacctcaaga attcgttcgt caggttgcac
      241 attcagattc cggatagccg attcacgccc ttcatggaaa actttcccaa agaccttatc
      301 atagaacccc gcgtaagagg tgtacacacg ctcaatcttg ttgggatcca tgctctcctc
      361 caaacctcac acgatccggc ccctgccttc ctcaccctct cagaggcagc agaccgctat
      421 ggcaacgcgg ggaaaaattg aggcgggaaa ttcatgtagt gcaaggcccg cccggaacgt
      481 agtccggcgt actctagcca atctcatctt catgagtcaa cagattcttg gagggtccgg
      541 gacggaaata ccgcctaaag ccgcacaggc agaacggtga tttcgtgcct tgcctccttg
      601 attcaggcct cattcctatg tgatagctac gtttatgata ctggccggtt tcttcgcagg
      661 aatcttattt atcggactaa tcgtcggtga ttggctgtat ttcatacggc tgaccccgga
      721 cgccatcagg tacggttgcc gtgtcgcgaa caacccggat cagtggcccg gaaccgcgct
      781 cacaacccta ggcgatcggt tcactccgga gggcgtgctg atgcttccac atggagtggc
      841 ctggtattat ccacaactct cccagattgc gattcgtcca cagtatcgat tgttctccag
      901 gcggttccgg accgcctggc cggtgaaagg gctgattcat ctttcgccgc atgaccaatc
      961 gctgaacacg ctgtgtgtga agcgtatgcc atggtcatcg gccctcatga cgctcctgtg
     1021 gtttgcactc gtcttgatcg ggtcgctgac cttcatggtg cagtttgtca tggatggagg
     1081 atttgcttca ctaggaggaa cccttacggg cgtggcggtc attgccctcg ccgcccttgt
     1141 gctggccttc ggactcgtca cagtggccct cgcctatcgg ctcgaagaca gccgcctcat
     1201 gaaagtctat ggcgagttgc gagatgccgt tgcgctcacg acttccgcct aacctaaccc
     1261 gtggggttct ttcctatccg aaaacaagtt taagaaatga tcgtattccg tcggcaagct
     1321 caacgtcgat cggttacgag ctaggccgac gatgatccca cgatgctttt ttgcaagacc
     1381 tgatgcgtca aaggccaagc ggaactgccg ccaccgcaaa ccgggatcgg ccaggattcg
     1441 ggtttgttct tggatcggga gtccatgtat catggtcgtc aatgtgcgga tggccttctc
     1501 cacgctttca tacggaggag cgagttgaag tccggcatag aacgtttcga ggtgatgacg
     1561 gaattccgct ttcagcgtct ggagtggatc gggtgttgtc aaagaatctt cggacataat
     1621 attacagacg atgatacggc atgacgaaac cacgtgacaa cttttccacg gaggaatgta
     1681 tgaatcggct gttcaatggg ctgtgtgcgt tgatgggagt tctcatactg actgcgtgct
     1741 cctcatatcg tcaccatcac ggcatgatga gtgatggcaa gagcgaggct tattggcaga
     1801 agggccagca agacatggaa gggctgatca atcgaaccgt caaagaaccc gatagggcga
     1861 agcaggtcaa tgtgatcgtc ggcgagatca tcaaggagct caagacaagc cgtgaacagg
     1921 aacgggccta tcaccgggac ctctatacct tgaatgccag ctatactgcg atgcccgatg
     1981 aattttcaaa aattctcaac gaggcgaaca ttcagcgaat gcagagttcg gccaaggtac
     2041 tgagcctccg gttcaaaatg aaggatctga tgaccgccga tgagtggaag gccctcaccg
     2101 ataaaatgct gtcctacagc agtcggtatc agcatggcga gagtggagca aaaacaggtt
     2161 actaaacaag gttgaggctg agattaggtt gagttcgagg gaaattgtag tttttttctc
     2221 agcttaacct tgacctgaac ctacggctgc aggtgcggct ttgagcggca caccgatcca
     2281 agtcgcgccg gcatcggttg aacgatagag tccgctgcca ttggtacctg catagagtac
     2341 ctgcggattc ttaggattca gggccaaggc gcgaatgttg agcgtcacaa tgccctggtt
     2401 caccgcctgc caagacttcc cgccgtcgga acttttccag acccctttcg gaccgccgac
     2461 atagagctgt aacggctccg ttggatgaag aagaatactg ctgataaagg gatcagtgag
     2521 ggattgtccg atccgttccc actgctctcc tttattcgtg gtacgaaaga ggccctttgt
     2581 cgtccccgca tatactgtgt caggagtcgc ccgatcgatt tcaatggcgt tgacacccag
     2641 cgccatggaa gccatcaact cgctatccgg aatcagcccg ttgttgactc ttttccacga
     2701 catcgcccca tcgtccgagc gatacatccc gcctgtcgtt ccaccgtaca ggattgtggg
     2761 atccttggga ttgatggcaa tggacgtcac gatatggact tccttcatcc cgttcattcg
     2821 ttcttcccat tcacggccgc cgtcctttgt aagaaaagca ccgacggtcg tcgcaatata
     2881 aatcttctcg ctcagggcgg gatggaacac aaactgattc acgaacgaga catgttcctt
     2941 caatccgaca ttatgtggaa gccaatgttg gccgccgtcc ggacttttgt agaccgcgtc
     3001 ccccatcgtg ccggcataaa tcgcggccgg aagctgagga tcgatcgcga gtgtagtgac
     3061 ccggcgtgcg ctgaagctgg ggaaccgctc ccaagtcccg ccgccatcgc gcgatttgta
     3121 gaccgcgtca ttcgtggcaa cgtagaggat atttgtattg gtcggatgga gcgcgatcga
     3181 cacgatggac tcgctctcct tttgacagcc gagcgagaag ctcagaagcg cgaacaagcc
     3241 caatgtaata atgatacgaa gcaacatcat tatggtggcg ggacctaatc ataggaatcg
     3301 gagatgagtc aagcaggagg acggaatgct aacattctgc ttagaattct ggactattac
     3361 gatgtggtct atgctttggt tgataataat caggggtttc agtagactcc acgataactg
     3421 gttctagtga tttagcactg taaccaagcg gcttcctgcc cagaaggcat gcatcatggc
     3481 cgaactcgtc gaacgcatca cagtcaaccc tgagcaatgt ggtgggagac cctgtattcg
     3541 tgggatgcga attcgagtcg tcgatgtgct cgatttattg gctgcgggac tctctcagca
     3601 acaggtcctt gaagaattgc cggaccttga aacggaagat atttccgcct gcctgaagtt
     3661 cgccagcagc cgcctcaatc atcccatcct cgccgcgtga agatctgggt agatgcgcag
     3721 ctttccccag tcatagctaa gtggctcagt tccacgtata gagtggatac cgtggcggtg
     3781 agaggcctga gattgcgcga tgcgcgagat cgggatattt ttctgtccgc acgagaagcc
     3841 cacgcgacca ttttaaccaa ggaccgcgac atggtcgatc ttgtcatccg acttggctcg
     3901 ccacctcaga tcctatgggt cacttgtggc aacacgtcca atgctaaact aaagaccatc
     3961 cttacaaagg catggcccgt agctgagaat cttctcaagg ctggagagaa ggtcgtcgag
     4021 atcagtgaca caacaggcta gagaaaagat agcgccggaa atatttgagg catttcatga
     4081 gtaagaaact aaccgataag gcactcgcca atttcgaggc caaacgcgat gtgtggcaag
     4141 aagtcctgga cggtgtacgg gagatcaaag ccggaggaga aaagcaaacg aaattgaagc
     4201 taagtcgtgc atactttgcg cattcatcat gcaattgtac tctgactcct atccgttcta
     4261 ttgcatttct attgcatttc gagtcgtaaa taacggtggc tcctgagaaa ctttttggac
     4321 cagaaaatat gtctgtatta gaagcactca agcgtaagga caggtcgctc gcactggagc
     4381 gattgtgcaa aggcgcagtt ttgaacgata aagatgaata tggaaatacg gcgttacact
     4441 atgcagccgc atttggctac atggatgtga tcgaaaagat tctagaggca agaagtgatc
     4501 tagcagtgtg ttgacaaacc tctcctcggc ccgtcgtgct tgtgataaga gaggcctcca
     4561 tcaggagggc cacaccgatg atgggtacat ccgatccgca gccgtcgatg ttttatcaca
     4621 tcaatctgga gcagtttgtg
//
LOCUS       DATNDD010000330         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1566072, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000330 DATNDD010000000
VERSION     DATNDD010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566072"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VN647_07955"
     CDS             <1..737
                     /locus_tag="VN647_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97000.1"
                     /translation="ERDLFVDVRGKVMNRLEYVVGIMNNNNFLANGIGSTMSGIGGAN
                     QPKAVYTRIRLFGDDVSFISFNMIQGTSNNAGTLINGRGKGHFDRYGVDFRYNPKFLP
                     GFMVYGEYWQGHDGSNATTVGTAANGACLDTCGGSGAPGVQRRTWYVLAKYLFTEGPL
                     ENFEPVYMYEEFDPNTRMSNDLYTRHIAGLNYYFENFPPKMQSKIQFNYEFRHHSGLG
                     PGVALNAATDPFAQNAFFIQWQLRYM"
     gene            811..1347
                     /locus_tag="VN647_07960"
     CDS             811..1347
                     /locus_tag="VN647_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWG97001.1"
                     /translation="MRIVAGLLVLSWAMVSACASAYKVFPSKAMEGVDRNFDFSRWRM
                     MADESESKKVQIGGRIVESQVSGDTVTIVVGQLPIVDHPAYGPKDNGKNNGDFVVIYR
                     GQIETPDLQRGNRVMVVGTTHPWKIVTVNDLSRSFPIVAAQCLHFWNTQGRDIADFPF
                     YEAGYMTLRQETVCTKNP"
     gene            1512..>2320
                     /locus_tag="VN647_07965"
     CDS             1512..>2320
                     /locus_tag="VN647_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97002.1"
                     /translation="MYDREGIRIGIEADPSVRPSSQTVLNNHPMDLTPTELESLLQVI
                     QVSGYSGTMVGLITKPEPVPLFTPKELSVISGHLATAFREAKPTERVSFSLPKPDVTY
                     SEDRTVGFLFFRGRYLHVVLTDHSSFIRADTSGGKDIRDTKGMRLWVAGPAHAALVPD
                     LEEPRWAHFEKVHISLVVKEILAQTEKIPAARMNQGGAVTPVPLPAAASPASRQGSAS
                     PEDLQLKIRELSGANQELKVRLDEQNKRTQQLQDQIEQLRRELPPSKSKGKS"
BASE COUNT          554 a          573 c          624 g          569 t
ORIGIN      
        1 aagaacgtga tttattcgtc gacgttcgtg ggaaggtcat gaaccgcctc gaatacgtgg
       61 tcggtatcat gaataacaat aactttctag caaatgggat cggcagcacc atgtcgggaa
      121 tcgggggagc caatcagcct aaggcggtgt acactcgcat caggctgttc ggagacgatg
      181 tctcgtttat tagcttcaac atgattcagg ggacctcgaa caacgccggc acgctcatta
      241 acggccgagg gaaggggcat tttgaccgct atggcgtgga ttttcgttac aacccaaagt
      301 ttctacctgg tttcatggtc tatggagaat attggcaagg acatgacggc tcgaatgcga
      361 cgacggttgg gacagcggcc aacggcgcct gtcttgatac ttgcggcggc tccggtgcgc
      421 caggggtgca gcgacgcaca tggtacgtcc tggcaaagta tctctttacg gaagggccgc
      481 tcgaaaactt tgagccggtc tacatgtatg aggaattcga ccccaatacc aggatgtcga
      541 acgatctcta tacgaggcat atcgccggcc tgaattatta ttttgaaaat ttcccgccaa
      601 aaatgcagtc taaaattcag tttaactatg agttccgaca ccattcagga ttgggacctg
      661 gtgtggcgct caatgctgcg acggacccat ttgcacaaaa cgcattcttc atacagtggc
      721 agttgcgata catgtaatga gtgtgcatta tgattcgtta gcttcattct tggtttgacg
      781 ctctatactg gaacagtgta ggaggtgaat gtgagaattg tcgctggtct tcttgtccta
      841 tcatgggcaa tggtgagcgc ttgcgcttcc gcctacaagg tgtttccctc caaggctatg
      901 gagggcgttg accgcaattt tgacttttcc cgctggcgta tgatggccga tgaatccgaa
      961 tcgaaaaagg ttcagattgg tgggcgtatt gtggagtcgc aggtatccgg cgacacggta
     1021 acgatcgtcg tcggtcagtt gcccatcgtc gaccacccgg cttacggacc caaggataac
     1081 gggaagaata atggagattt tgtcgttatc tatcggggac aaatcgagac gccggatctc
     1141 cagcgtggca atcgagtgat ggttgtaggg acgactcatc cgtggaaaat cgtgaccgtt
     1201 aacgatctct cccgaagctt cccgatcgta gcagcccaat gtctccattt ctggaacacg
     1261 caagggcgag acattgcaga ttttccattc tatgaagcag gatacatgac ccttaggcaa
     1321 gagacggttt gtacgaagaa tccatagttg tggtcttcaa tataagactc atgaatattg
     1381 ccaatggtag taaaggtcca cagtttgcca ggtttcattc actcatcacg tcgacccctt
     1441 tctgctgctt gtctctgatc ctcacaatcg gaaatctatc tggctgtgcc ctcattgacc
     1501 atggacgatc ggtgtatgac cgagagggta tcaggatcgg tattgaagct gacccctcag
     1561 ttcgcccatc cagccagacg gttttgaaca atcacccgat ggatctcact cccacagaac
     1621 tcgaatcgct gcttcaggtc attcaggtca gtggctatag tgggaccatg gttggactga
     1681 tcacaaagcc ggaaccggta ccacttttta cgcccaagga gttatcggta atttcgggac
     1741 atctcgccac ggccttccgg gaggcgaagc ccacggagcg agtgtctttc tcgttgccga
     1801 agccggacgt gacttatagt gaagatcgaa cggtcggctt tttgtttttt cgtgggcgct
     1861 atctccatgt ggtcctgacc gaccattctt cctttattcg tgccgatacc agcggcggga
     1921 aagatatccg agatacaaaa ggtatgagac tctgggtggc cggcccggct cacgctgctc
     1981 tggtgccgga tcttgaggaa ccacgatggg cgcatttcga gaaggttcat atttctctag
     2041 ttgtcaaaga gatcctggct cagacggaga agattccagc tgcgcgcatg aatcaaggag
     2101 gagcggtgac gccagtcccc ctgcctgctg ccgcatcacc ggcaagccgc cagggcagtg
     2161 cctcgcctga ggatctgcaa ctcaaaattc gagaactctc cggtgccaat caagagctca
     2221 aagttcgact tgatgagcag aataagcgta cgcagcagtt gcaagatcag atagaacagt
     2281 tgcgccgtga acttcctccg tccaaatcca aggggaaatc
//
LOCUS       DATNDD010000331         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3302290, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000331 DATNDD010000000
VERSION     DATNDD010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302290"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VN647_07970"
     CDS             <1..393
                     /locus_tag="VN647_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97003.1"
                     /translation="QHGERTARLLLRRYGVVFRDMLARESLVQSWRDLLVQYRRMEMA
                     GEVRGGRFVNGFTGEQFALSEAVEALRTIRKKSPSAGTEHEIKLSATDPLNLAGVILP
                     GPRVPAVPTNFLVFKDGALSRTVSRTAR"
     gene            complement(462..866)
                     /locus_tag="VN647_07975"
     CDS             complement(462..866)
                     /locus_tag="VN647_07975"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HWG97004.1"
                     /translation="MQRMPAHPLILTGVLATLLPWNGCTTKATIDQTTDTTSNITGTT
                     SGAAWWSEDGQIKPDFKATAFVTFNQANLSQDLAAGRGEYLGSVSRLLGVPTDRQPAF
                     FSAAQANYAQMADLNSEALFTLLHKTSKPFVY"
     gene            1044..1271
                     /locus_tag="VN647_07980"
     CDS             1044..1271
                     /locus_tag="VN647_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HWG97005.1"
                     /translation="MANLAEVMKGAMSLDVRDRAALAEKLLASLDEVSEAEADLLWAE
                     ESQRRLDQYHAGQADAVSGDAVHAKVNKLLR"
     gene            1268..1567
                     /locus_tag="VN647_07985"
     CDS             1268..1567
                     /locus_tag="VN647_07985"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWG97006.1"
                     /translation="MMSLRYHEAAEEELLNEIGYLELQAQGLGRRFFAEIRRAEDRIL
                     QFPQSAQEIRPGIRKTILRTFQYSLIYSIEPEEIVVLAVAHHRRRPDYWAGRLSE"
     gene            1806..2345
                     /locus_tag="VN647_07990"
     CDS             1806..2345
                     /locus_tag="VN647_07990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG97007.1"
                     /translation="MKDSTYGVAALERALTFIGSGRHALDVGCECEGRFLRVLLERGF
                     DCTGLDISQEMIALAKQRYPAVLFMTGDVCTWSLPRQYDLITAWDSLFHLPLEAHEPV
                     LGKLCKGLSLDGVLLFTCGDGKEPGSIQGEFGGKRFEYSSLGVVEFIRLLHHAGCTIQ
                     HLERDQHPLNYVYIVAKKL"
     gene            complement(2460..>2762)
                     /locus_tag="VN647_07995"
     CDS             complement(2460..>2762)
                     /locus_tag="VN647_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97008.1"
                     /translation="SPSRLLAVAEEPAQQPSAKERLNQVLGQEGGIHVYKDDQWNVES
                     TIVLPNGERIIKVQPPPSPSMNLGPPLQLHNQTFQLPSPTPAPTHPSAPEFPQRAR"
BASE COUNT          628 a          666 c          812 g          656 t
ORIGIN      
        1 cagcacggcg agcgcacggc tcgcctgtta ctacgtcgct atggggttgt gtttcgcgat
       61 atgctggcgc gggaatcgtt ggttcagtcg tggcgtgatt tgcttgtgca atatcggcgc
      121 atggagatgg ccggggaggt ccgcggcggc cgatttgtga acggattcac cggggaacaa
      181 ttcgcgttgt cggaagcggt ggaagccctg cgaacaatcc gaaaaaagag tccctccgca
      241 gggacggagc acgagatcaa actctcagcc acggacccat tgaatctcgc cggggtgatt
      301 ctgccaggcc ctcgtgttcc ggcggtgccg accaattttt tggtgttcaa ggacggcgcg
      361 ctttcccgca cggtgagtcg gacggcaagg tgacgcgacg cattctgttg ccgagcagac
      421 cggcccggca acagtccgac aacctgcatg aattcaatag gtcagtacac gaacggtttg
      481 gacgtcttgt gcaaaagagt gaacaaggct tcggaattta ggtccgccat ttgggcataa
      541 ttcgcctggg cggcagaaaa aaatgccggt tgtcggtcgg ttggaacacc caacagacga
      601 ctcacggatc ccagatactc tcctcttccg gctgccaggt cctggctcag attcgcctga
      661 ttaaaggtca caaacgccgt ggctttgaag tccggcttga tctggccgtc ctcgctccac
      721 caggccgctc ccgaggtcgt gccggtgatg ttagacgtcg tgtcggtcgt ctggtcgatg
      781 gtggctttgg tcgtacaacc gttccatggt agaagagtcg cgagcacacc agtaagaatc
      841 aacggatggg ctggcattcg ttgcatgagc atcctccttg gcttgagtag agaaaaccac
      901 gcctaggttt ttaccatagc aggagcgcat tcggtcatca agtcggatat ggggcgaacg
      961 gtactcgccg aggggacgtt gtgcgttgac aagcgctcag aatcgagcaa gaatcatagc
     1021 tcgaggttga caaggaggag gaaatggcaa acttggcgga agtcatgaaa ggcgcgatga
     1081 gcctggatgt ccgagatcga gccgctctag cggagaaact tcttgctagc ctggatgagg
     1141 tgagcgaggc tgaagcggat cttctctggg cagaagaatc gcaacggcga ttagatcagt
     1201 atcatgcagg tcaggccgac gccgtttcag gggacgcagt tcatgccaag gtgaataagc
     1261 tcttgcgatg atgtcgcttc gatatcacga ggcggcggaa gaagagttgc tgaacgagat
     1321 cggctatctc gagttacagg cccaagggtt gggccgacga ttctttgcag agattcgacg
     1381 agctgaagac cgcatcctac aatttcctca gtcggctcaa gagattcgac ccggcattcg
     1441 gaagaccatc cttcggactt ttcagtattc gctcatatat tcgattgaac cagaagaaat
     1501 agtagttttg gcggtggctc accatcgtcg gcgtcccgac tattgggccg gtcgcttgag
     1561 tgaatagagc ataagttcat tcattgcgct tctatggagc agcttgtttg ccgcgttgtg
     1621 gaaccgcaaa tcaatcgatg gtggagtgcc gccaagcggt tcaaatacca tcgtgtgcta
     1681 ctcgtcctga cgcggaatgc caatctctgc gaaatagaat ccacgaagaa tgcttactta
     1741 gagtccatgg acccaaagta cactgccaac agtacgatgc catcgcggta tggtggcttc
     1801 aacagatgaa agattcgacg tacggcgtgg cggcgcttga gcgcgccctt acattcatcg
     1861 ggtcagggcg acacgcgttg gatgtcgggt gcgaatgtga ggggcgattt ctcagagtac
     1921 tcttggagcg aggctttgat tgcaccgggc tcgacatctc gcaagaaatg atagcattgg
     1981 caaagcaacg ctatcccgca gttttgttta tgacgggtga tgtctgcaca tggtcactac
     2041 cacggcagta cgacctgatc acggcttggg atagcctctt tcatcttccg ttggaggcac
     2101 atgagccggt tttaggaaaa ttatgcaaag gattgtcact ggatggcgtg ctgctattca
     2161 cctgtggcga tggaaaggag ccaggaagca ttcagggaga attcggtggc aagcgatttg
     2221 agtacagctc attgggtgtt gttgagttta tccgactcct acatcatgcc ggctgtacga
     2281 ttcaacatct tgaacgcgat cagcatcccc tgaattatgt gtacatcgtg gctaagaaat
     2341 tatagagcat caagtcaact aagatccctg ttcgagtaag ggcttaaacc tgcaatttgc
     2401 atttgaatgc agtctcaatc ttccggcgct gctcgttcaa cattatcatc gaattacttc
     2461 tagcgagccc tctgtggaaa ctctggagcg gatggatgag ttggagcagg agttggagaa
     2521 ggaagttgaa atgtctgatt gtgcaattga aggggagggc caaggttcat ggatggactt
     2581 ggcggtggtt gcaccttgat gattcgttca ccgttaggca gaacgatcgt gctttcgaca
     2641 ttccattgat cgtctttgta gacgtgaatg ccaccttcct gacccagtac ttggtttaac
     2701 cgttccttcg cgctcggctg ttgagccggt tcctctgcaa ctgctagcaa ccggcttggc
     2761 ga
//
LOCUS       DATNDD010000332         3984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2141993, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000332 DATNDD010000000
VERSION     DATNDD010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141993"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VN647_08000"
     CDS             <1..760
                     /locus_tag="VN647_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWG97009.1"
                     /translation="TVQHVTGAPLGFAGPVGLRHVRILADYAVKGMKNIVIGANKSDT
                     HYQNANLDRDFSVELFADLRNAQAGDPSPRGRGVLTLAKGIEVGQVFLLGTKYSHKMN
                     AAILDDQGKERLAIMGCYGIGVGRTAAAAIEQNHDEKGIIWPFPIAPFHVHLLTVSQS
                     EKTTEAAVRLYTDLAAAGIEVLWDDRADRAGVKFNDADLIGVPFQIVIGDKGLADGVA
                     EIKIRRTGIKSRIARGELVVHLRKLSTESDPSAT"
     gene            977..3283
                     /locus_tag="VN647_08005"
     CDS             977..3283
                     /locus_tag="VN647_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWG97010.1"
                     /translation="MTQNVHELQQKVEHAEHVKRITIQIHAASNLDHILLDLHKDILS
                     LFDAEDLTLFAFDSEKKEIFSKLPHIDSVEEVRIPITEQSLAGFCAKYLRPVNIADAY
                     NIAELRAIHPSLLHDTSYDKRTGFKTKQVLTYPIVADNKYLMGVLQLLNKKGGSRFTR
                     RDEEAVDEIAKALGIAFFNLRKISKKNPTKFDFLVGTNRITQNELDQVIADSRKGMSD
                     LESILIEKYKVPKLDIGRSLAQFYKCPYIEYSERTLVDVELLKNLNVDYLKKNHWMPL
                     KRDRMAIEILTDDPGDLDRVQDIKRTFPGLNIRFAVSLRRDIAQFLSSATGQSDGGGG
                     RKLDENVSDILGELVTEAQAEAMEDSAAGGGLDENDSAIVRLANQIIADAYRQNASDI
                     HIEPYGEKRETLVRFRVDGDCFEYMKIPQSYRRAIVSRLKIMASLDIAERRKPQDGKI
                     KFKLSETKEIELRVATLPTAGYNEDVVMRILAASEPLPLDKMGFSERNLKVLKEISEK
                     PYGIILCVGPTGSGKTTTLHSVLGNINTPDIKIWTAEDPVEITQYGLRQVQVQPKIGL
                     TFANAMRAFLRADPDVIMVGEMRDKETADTGIEASLTGHLVLSTLNTNSAVETVTRLL
                     DMGCDPFSFADAMLGVLAQRLARRICKDCKEQYIGSKEEYEELRSAYGSEYWDKLGIK
                     QDNTFRLSRGKGCETCNRSGFKGRVALHELLLGTDHMKRMVQQKARTEEMLKIALEEG
                     MTTLVQDGIQKVLHGHTTYKEVKAVAIK"
     gene            3381..3635
                     /locus_tag="VN647_08010"
     CDS             3381..3635
                     /locus_tag="VN647_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97011.1"
                     /translation="MIRITSLFLASLLLLAGCESPDRILTNAERVLGSRTGRTVLDIA
                     TGKDPKLILKERVDAYQRDHLPSITDCAIICRTRERWIIW"
     gene            complement(3733..>3984)
                     /locus_tag="VN647_08015"
     CDS             complement(3733..>3984)
                     /locus_tag="VN647_08015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97012.1"
                     /translation="IIYDVSAKGMRVMTEAVITPGDRIAMRLRLPDQAALMVIETATV
                     RWGKEQTYGVEFEGLSPNDNKHLQAFMNHQSRSGQALAV"
BASE COUNT         1012 a         1129 c         1027 g          816 t
ORIGIN      
        1 gacagtccaa catgtaacag gggctccgct cggatttgcc ggaccagtcg gtctgcggca
       61 cgtacgaatt ttggcggatt acgccgttaa gggaatgaaa aacatcgtca tcggtgccaa
      121 caaatccgac acccactatc agaacgccaa tctcgaccgc gatttctcgg tcgaattatt
      181 tgccgatctt cgcaatgcgc aagccggaga cccttctccc cgaggacgag gtgtgctcac
      241 gctcgccaaa gggatcgaag tcggccaggt gttcttactc ggcaccaagt acagccacaa
      301 aatgaacgcc gccatcctcg acgatcaagg caaagagcgc cttgccatca tgggttgcta
      361 cggcattggc gtcggccgga cggccgccgc ggcgatcgag caaaatcatg atgagaaagg
      421 catcatctgg ccgtttccca tcgcgccatt ccatgtacac ctcctgacgg tcagccaatc
      481 ggaaaagact accgaggctg ccgtacgtct ctataccgat ttggcggcgg caggcatcga
      541 ggtattgtgg gatgaccgtg ccgatcgcgc cggcgtcaaa ttcaacgacg ccgacctcat
      601 cggtgttccc tttcagatcg ttattggcga caaaggtctt gcggacggcg tcgcggagat
      661 caagattcgg cgaactggaa tcaaatcccg cattgcacgc ggtgagcttg tcgtccatct
      721 caggaaactc tccaccgaat ctgatccatc cgcaacttga tccaggcctc ggcttccgac
      781 tgagaagcgc ctcacatttt ttggaaatgg aggacaaaaa atggattttt cttgccttcc
      841 cacgtcattc cgttagactg aaattcgatc cgcagatcgg tgacctctac ctggcggcac
      901 ctgctgatcc ggtatataac ctgattcatt cacgtttcga cctccgagaa aatacagccg
      961 gatgcacacc aagccgatga ctcagaatgt ccacgaactg cagcaaaaag tcgaacacgc
     1021 ggagcacgtc aaacgcatta ccatccagat tcatgcggcc agcaatctcg accacatact
     1081 tctcgacctg cataaagaca tcttaagtct gttcgacgca gaagatctga cgctttttgc
     1141 gtttgactcc gaaaagaagg aaattttctc caagcttcct catatcgaca gcgttgaaga
     1201 agtccgcatc cctattaccg agcagagtct cgccgggttt tgcgccaaat acctccgccc
     1261 agtcaatatt gccgatgcct acaacatcgc cgagctccgc gcaatccatc cgtcgctgct
     1321 ccacgacacg tcctacgata aacgcaccgg cttcaaaacc aaacaagtcc tgacctaccc
     1381 catcgtcgcc gacaacaaat atctgatggg agtcctccaa cttctcaata aaaaaggcgg
     1441 aagtcggttt accagaaggg acgaagaagc ggtcgacgaa atcgccaaag cgctgggaat
     1501 cgcttttttc aatctccgaa agatatccaa aaagaatccc acgaagtttg actttctggt
     1561 cgggaccaat cggattacgc agaatgaact cgaccaggtc atcgccgact cccgaaaagg
     1621 gatgagcgat ctagaaagca ttctgatcga aaaatacaag gtccccaaac tcgatatcgg
     1681 tcgatcgctc gcccagttct ataagtgtcc gtatatcgaa tatagtgaac gcaccctcgt
     1741 cgatgtggaa ctgctgaaga acctcaatgt cgactacctg aaaaagaatc attggatgcc
     1801 gctcaagcga gaccgcatgg cgatcgagat tctgaccgac gatccgggtg accttgatcg
     1861 cgttcaagac atcaagcgga cgtttccggg cctcaacatt cggttcgctg tcagtcttcg
     1921 ccgcgatatc gcccaatttc tgagttccgc gaccggacag agcgacggcg gcggagggcg
     1981 caagctcgac gaaaatgttt ccgatattct cggcgaactg gtcaccgagg cacaggccga
     2041 ggcgatggaa gactccgctg cggggggagg attggatgaa aacgacagcg cgatcgtccg
     2101 tctggccaac caaatcattg ccgacgctta tcgccaaaat gcatctgaca ttcacatcga
     2161 gccgtacgga gaaaaacgcg aaaccctggt ccgtttccga gtcgacggtg actgtttcga
     2221 gtacatgaaa atcccgcaga gttaccggcg agccatcgtg tcccgactca agatcatggc
     2281 cagcttggac attgccgaac gccgtaaacc tcaagacggg aagatcaagt tcaaactctc
     2341 ggagacgaaa gagatcgagc ttcgggttgc gaccctcccc actgccggat acaacgaaga
     2401 tgtggtcatg cgtatcctcg cggcgagcga accgttgcct ctcgacaaga tggggttttc
     2461 ggaacggaac ctcaaggtgc tcaaggagat ttctgaaaaa ccctacggca tcattctgtg
     2521 tgtcgggccg accggttccg gaaaaacgac cacgctgcac tcagtcctgg gaaacattaa
     2581 caccccggac atcaaaatat ggacggccga ggatccggtc gaaattacgc agtacggcct
     2641 gcgtcaggta caggtccagc cgaagatcgg cctgacattc gccaatgcga tgcgcgcatt
     2701 tcttcgggcc gaccccgacg tcatcatggt gggagaaatg cgcgataaag agacggccga
     2761 caccggcatc gaagcgtcgc ttaccggcca tctggtgttg agcacactga ataccaacag
     2821 cgcggttgaa acggttaccc gcctgctcga catgggctgt gatccgttca gttttgccga
     2881 tgccatgctg ggtgtgctcg cacaacgatt ggctcgccgg atctgcaaag actgtaagga
     2941 gcagtacatc ggaagcaaag aggagtatga ggaacttcga tcggcgtatg ggtctgagta
     3001 ttgggataag ctcggcatca agcaagacaa tacgttcagg ctgtcccgtg gaaagggctg
     3061 cgaaacatgc aaccggtccg gcttcaaggg acgggtcgcc ttacatgaac tcctcttggg
     3121 cacagaccac atgaagcgaa tggtccaaca aaaggcacgc accgaagaga tgctgaaaat
     3181 cgcgcttgaa gaagggatga cgacgcttgt gcaagacggc attcaaaaag tcttgcacgg
     3241 ccacacgaca tacaaagaag tcaaagccgt cgccatcaaa tagtgctttc gatttacccg
     3301 gtacgaccgg ttttctggag acagtcccct tcattcccac ccacaaccgc gtagaatgcg
     3361 ccgcccagca tggagcactg atgatccgga tcacatcgct tttcttggcc tcgctgctcc
     3421 ttctcgcagg atgtgagtcc ccggaccgca tactgacaaa cgctgaacga gtgctcggca
     3481 gccgaaccgg caggaccgtg ctcgacattg ccacaggcaa agaccccaaa ctaatcctca
     3541 aagagcgcgt cgatgcctat cagcgcgacc acctgccgtc tataaccgat tgcgcaataa
     3601 tctgccgtac cagggaacga tggattatct ggtgaagatc gtcaccttcc gcaaacagtt
     3661 catctcccta cgcgaagaca atgatcgttg agcgccggca ggatgttcag aaatactatg
     3721 caagggaggg ggtcagacgg ctagagcctg gccggatcgg gactggtggt tcatgaaggc
     3781 ttgcagatgc ttattgtcgt tgggagacag tccttcgaac tctactccgt aggtttgctc
     3841 tttaccccac cgaaccgtgg cggtttcgat gaccattaag gctgcctgat cgggcaatcg
     3901 caaacgcatg gcgattcggt ctcctggtgt gatcaccgct tccgtcatga cacgcatacc
     3961 ttttgccgac acgtcataga tgat
//
LOCUS       DATNDD010000333         4146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2412917, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000333 DATNDD010000000
VERSION     DATNDD010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412917"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..571
                     /locus_tag="VN647_08020"
     CDS             227..571
                     /locus_tag="VN647_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098609 - cell-cell adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin domain-containing protein"
                     /protein_id="HWG97013.1"
                     /translation="MGVMSAYAAADIELKTVDKACWAEIYEDTDFDVNDPHVKIQGPT
                     DIATLKDYSGRNWNNEIESIVVGPNAHIKAYSDKDFKGTELELTPNKRANDLSKLNMS
                     DEIESLKVTCGG"
     gene            complement(1006..1701)
                     /locus_tag="VN647_08025"
     CDS             complement(1006..1701)
                     /locus_tag="VN647_08025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HWG97014.1"
                     /translation="MKRAVRYSWGRIRQSSQCPCFYFIATLLTVVLGGEAFAFIESGN
                     SPHERTISILGVTVSSSGPIGTVTSIALTFQERTDQTGLMIGFTGGPGRLSRMAQTSV
                     QQAIYRVAHTAKLSTDSWTVTISVPNPITIYGDSLSAMVALTVIALAKQDSIMDDCII
                     TGGIAPDGHISKAGGLSLKLAAAGKSHIHHVLVPDEFDPSEPDWQTPFLMHVSPVDSV
                     QQAYQTLTGSPIN"
     gene            complement(1756..2157)
                     /locus_tag="VN647_08030"
     CDS             complement(1756..2157)
                     /locus_tag="VN647_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWG97015.1"
                     /translation="MRNGNYYAGQTDAAGWSAFLAGALIGAGVALLFAPQRGSELRGM
                     LRDYANHATDDLMDKGQEAWDTAVERGEQYYHRGEEAIRDAGRSAKEFARQGRETMSD
                     AGRSAKEFAEQEKNAVEESADEATGTVDSSR"
     gene            complement(2287..2454)
                     /locus_tag="VN647_08035"
     CDS             complement(2287..2454)
                     /locus_tag="VN647_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97016.1"
                     /translation="MGVGNHALDRMGTIAMWLAALGIVTLGLSIAGGVTGTAETPGVE
                     VQAGQYRERAA"
     gene            2639..2785
                     /locus_tag="VN647_08040"
     CDS             2639..2785
                     /locus_tag="VN647_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97017.1"
                     /translation="MVGRSLFAGAHAPGKERLMPESISHGGGQEQDKIATLAASSSGP
                     TRSQ"
     gene            complement(2810..>4146)
                     /locus_tag="VN647_08045"
     CDS             complement(2810..>4146)
                     /locus_tag="VN647_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97018.1"
                     /translation="KARSSSPAFLDQPRDGKNSGFDFYRDPLGAEKPLQEPDEIMKKD
                     IAAKPKVMETQRKFLERRYHLKPKLDSEAKMSRGKPLPVGPTTRLPESMTWERLAGLS
                     PEEIKKQGLFPYPSLPHPLQSPGGMVFPPMQIDMFPRLERFDVDHDLPEAFLPEFPPA
                     IFLSNRPELGDVSRGEVISINNYYRLFKDILTPVQLDGLRMLLTPFPQEEFNPTDDRK
                     SLHPSLGVTCFDCHVNGHTTGQFHLNPDTRPEERRFRLDTTSLRGVFNQQIHSSKRSL
                     RSIEDFTEFEQRTAYFNGDEIHAIKKGMNILDRIAVTHMAQMQNMLDFPPAPKLDPMT
                     GRLDPKKATENELRGEKIFFGKGQCAGCHTPPFYLDNKMHDLHLERFLKDEPGDGPIK
                     TFTLRGIKDSPPYFHDGRLLTLEDTVEFFNLVMGLQLSPDEKKDLTMFLRTL"
BASE COUNT          920 a         1133 c         1117 g          976 t
ORIGIN      
        1 acactttgac ttacgtgaag catttctgaa aactatgatt ccgcagctgc gcatagaaac
       61 cccttcaata ttcgggtaat gactttcgtt accacgcaat cgtcaaggta tagcctttgc
      121 agcctttcat atatgtcggg cgatgagtgt gctttaaaac cttagtgtaa tccaatagga
      181 ggtcacaatg atgagactag cgctaatttc agctggattg gtgggtatgg gtgttatgtc
      241 tgcctatgcc gccgcagaca tagaattgaa gacggtggac aaggcttgtt gggctgagat
      301 ctacgaggat accgactttg atgtcaatga cccgcatgtc aaaattcagg gccctacgga
      361 tatagcgacg ctgaaagatt acagcggccg aaactggaac aacgagattg aaagcatcgt
      421 cgtcggtccc aatgcgcaca tcaaggccta ttcggataag gatttcaaag gaaccgagct
      481 ggaattgacg ccgaataagc gagccaacga cttgagtaaa ttgaacatga gcgatgaaat
      541 cgagtcgttg aaggtcacgt gcggtggatg acccgtcgcc ggactggtgt cgtgagttcc
      601 tatgtatagc tttgatccgc agacgtagcg gaaacattat ctaagcataa tgtaaaaatt
      661 gcatgttcga aggctccgtc cttgaaaaaa ggacggagcc ttttcatgga ccaagcgcac
      721 aggtccataa agaaagagcg ctaggaattg ccggccgcga gacttcagtg ccccccgaat
      781 cgcccgccgt cgaaaacttt accgcctgtc gtacgaaggt tgccgagact ttccattgct
      841 gatacggcgg accccagcgt gacttcaaat cagtggtccg agagacgcag caacctaaag
      901 atgctaccca ggataaccgg accctgccga cggttcctct taatagactc ccacgccaga
      961 tgaaagaggt cggccccgtt aactgccggg cagacagctt acatcctagt taattggact
     1021 tccggtgagc gtttgatatg cctgctgtac agaatctacg ggacttacgt gcatcagaaa
     1081 cggtgtctgc caatccggtt cagacggatc aaattcatcc ggcacgagga catgatggat
     1141 atgcgacttg ccggcggccg caagcttcag cgagagcccg ccggctttgc tgatgtgtcc
     1201 gtccggcgca atcccacccg tgatgatgca atcatccatg atagagtctt gcttggcgag
     1261 ggcgatcacc gtgagcgcca ccatggcgga taagctgtct ccatagatag tgatgggatt
     1321 gggaacggat atggttacag tccatgaatc agtggatagt ttggccgtat gtgcgacccg
     1381 atagatggct tgctgaacgg aggtctgagc catccgcgac aatcttccgg gtccccccgt
     1441 aaagccgatc atcaagccgg tttgatcggt tcgttcctga aacgtgagcg ctatgctggt
     1501 gaccgttccg atcggtccgc tgctcgatac cgtcactccc aatatcgata tcgtccgctc
     1561 atgcggagag tttccgcttt ctataaaggc gaaagcctct ccgccgagca cgaccgttag
     1621 taacgtcgca atgaagtaaa agcagggaca ctgagaactt tgccttattc gaccccagga
     1681 atagcgcacc gcgcgtttca tcttctccca cgttcccgga ttcttttcct tcccatgata
     1741 gtcgggcgtc cgatgctatc gactgctatc gacggtgcct gtcgcttcat cggccgattc
     1801 ttccaccgcg ttcttttcct gttcggcgaa ttccttagct gaacgtccgg catcgctcat
     1861 cgtctcccgt ccctgccttg cgaattcctt ggccgaacgt cccgcatcgc ggatcgcctc
     1921 ttctcccctg tgataatatt gctcgcctcg ttccactgcg gtatcccatg cttcctggcc
     1981 cttatccata agatcatctg tcgcgtggtt ggcataatcg cgcaacatgc cgcgaagctc
     2041 cgagccccgc tgaggagcaa acagcagcgc gacgccggcc ccgatcaggg ctccggccag
     2101 aaaagccgac catcccgctg cgtcagtttg tccagcgtaa tagtttccgt tgcgcataat
     2161 atcgttctcc ttttggcgtt taaaacaaat tgaatatgtg caccgtcagt cccgatagtg
     2221 agggttcgtg tccttcccat cggtggagga ctatttcaga catctttctg ttagcttgcc
     2281 catcgtctat gccgcccgct cacgatattg ccccgcctgc acttcgacgc cgggcgtctc
     2341 cgcggtgccg gtcacgccgc cggcgatcga tagacccaac gtgacgatcc ctagcgctgc
     2401 cagccacatc gcaatggttc ccatacggtc caatgcgtgg ttgccgactc ccacggattg
     2461 ttccttcact tgttgagcct tgtctttcag gggctcaatg aaaccgatag tcgattggac
     2521 ccgatgagtt gagctcgccg aaaagatcaa aacagataga agcgccgaaa acattagaag
     2581 acatgcgctg acaccgaata gtgatgttca gcagacaggt tgtctttcaa aagacaggat
     2641 ggtcggtcga tctcttttcg ccggcgctca tgcgccggga aaagagcgac tgatgccgga
     2701 gagcatcagt cacggaggcg gccaggagca agacaagatt gcgaccttgg ccgcttcctc
     2761 ctccggcccg acgcgctcgc aatagcctcg agccgatcgc catacacttc tacagggttc
     2821 tcagaaacat cgtcagatcc ttcttttcat cgggagacaa ttgcagcccc atcacgagat
     2881 taaaaaattc caccgtgtct tcgagcgtca acaaccgtcc gtcatggaag tacgggggcg
     2941 agtctttgat gccccgcagc gtaaacgtct tgatcgggcc gtcgcccggc tcatccttga
     3001 ggaaacgttc gagatgcaga tcgtgcatct tgttgtcgag gtagaacggc ggcgtatggc
     3061 acccggcaca ttgacccttg ccgaaaaaga ttttttctcc ccgtagctca ttctccgtag
     3121 cttttttcgg atccagccga ccggtcatgg ggtcgagctt gggggcgggc ggaaaatcga
     3181 gcatgttctg catctgcgcc atgtgggtca ccgcaatgcg gtccaagata ttcatccctt
     3241 ttttgatggc atggatttcg tcgccgttga agtaagctgt ccgttgctca aactccgtaa
     3301 aatcctcgat cgagcgcaag ctgcgtttag atgagtggat ctgttgattg aatacgcctc
     3361 gtaggctggt cgtatcgagg cgaaagcgcc gctcctcggg tctggtgtcg gggttcaaat
     3421 ggaactgtcc ggtcgtatgg ccgttcacat ggcagtcgaa gcaagtcacg cccaagctcg
     3481 gatggagact tttgcggtca tcggtcgggt tgaattcctc ctgtggaaac ggtgtcaaca
     3541 gcatcctcaa tccatcgagc tggacggggg taaggatatc cttgaacagg cgatagtagt
     3601 tattgatgga gatcacctcc ccgcgtgaca cgtcgcccag ttcaggacgg ttgctcaaga
     3661 agatggcggg gggaaactca ggcaggaagg cctcgggaag gtcatgatcg acatcgaacc
     3721 gctcaagccg tgggaacatg tcgatttgca tcgggggaaa gaccatgccg ccaggggatt
     3781 gcagcgggtg cggcagcgaa ggatagggaa agagtccctg tttcttgatc tcttcaggac
     3841 taagcccggc gagccgttcc catgtcatgc tttccggcaa gcgcgtcgtc ggacctaccg
     3901 gcagcggttt tccccgcgac atcttggctt cggaatccag tttcggcttg agatgatagc
     3961 gccgctccag gaactttcgt tgcgtctcca tcaccttggg cttcgcggcg atatcctttt
     4021 tcatgatctc gtccggctcc tggagaggct tctccgctcc caatgggtcg cggtagaaat
     4081 cgaaacctga gttcttgccg tctctcggct gatccaggaa agcaggggaa gaagatcttg
     4141 ccttct
//
LOCUS       DATNDD010000334         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1416081, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000334 DATNDD010000000
VERSION     DATNDD010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416081"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VN647_08050"
     CDS             complement(<1..226)
                     /locus_tag="VN647_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97019.1"
                     /translation="MNTKIGDNPRRSKSLVLCIAIALSVVSTPSAFAETYVSGMVGAT
                     LAPDTVRGRVNDPSFVGLPEGTSISNIQLNS"
     gene            381..953
                     /locus_tag="VN647_08055"
     CDS             381..953
                     /locus_tag="VN647_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG97020.1"
                     /translation="MARPAFGQLREALLQSASGEILEIGFGTGLNLRHYPTTITRLFI
                     VDPAILLPARVARRMAAAPYPIQTIHVPAEDLPFPERQFDTVVSTWTFCTIPDPVLAL
                     QEVGRVLKPGGKFLFLEHGRSDDRKIAAWQDRLNPVQKVIGCGCNLNRPIDRLVTQAG
                     LTIVHLDRFSMQDVPRLGGEMYRGTATRQS"
     gene            complement(950..1726)
                     /locus_tag="VN647_08060"
     CDS             complement(950..1726)
                     /locus_tag="VN647_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWG97021.1"
                     /translation="MPDGNIGFFSKTTVSPSRSTSDILLPMVQSSVLSPQHFPDWTQI
                     DDVLLDMDGTLLDRHFDNFFFEEELPRRYAVLHALPFEEARDRLMAMYRSVEGELAWT
                     DLDYWTKRVGIDVVAMHKELDHMIGFLPGAEEFLRRLQQLEKRVTIVTNAHSTGVSVK
                     VAKTGLDRYVNRIVDAFEVGSLKMRPEYWPNCQRLLGFDPSRSLFMDDDEGCLMAAKE
                     FGVAHLVHSAKSSSQLPATLLPQFLSISGFAPLLNGKPPV"
     gene            1754..>2578
                     /gene="der"
                     /locus_tag="VN647_08065"
     CDS             1754..>2578
                     /gene="der"
                     /locus_tag="VN647_08065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWG97022.1"
                     /translation="MSSHSALSPQHSALPLVAIIGRPNVGKSTLFNKILGAKIAIVDD
                     VPGVTRDRNYADAAYRNRKFRLVDTGGLDLSSSDSMLSLIRRQSELAIAEADILIVLL
                     DGRSGLTPPDHEVVRLLRGVTKPLFYAINKIDTPKSEPLLADFYRLGTDQLYPVSAEH
                     GLGVAELLDAMYPLLPSADESDELQQLPRVAMVGRPNVGKSTLVNTLLGEERVVVSDV
                     PGTTRDPIDSLVTHHDQRYIFTDTAGIRRRGKVDRGVEGYSVLRSLRAIGQSDIAVL"
BASE COUNT          571 a          759 c          729 g          519 t
ORIGIN      
        1 agctgttcaa ctggatgttc gaaatggacg taccctcggg aaggccgacg aaactcggat
       61 cgttcactcg cccacgtacc gtatccgggg caagggtcgc cccaaccatg ccagagacat
      121 aggtctcggc gaaagccgaa ggagtcgaaa caacacttaa ggcaatcgcg atacagagaa
      181 ccaggctctt ggaccgtcgc gggttatccc cgattttcgt gttcatcgaa atatctccta
      241 ggatggccaa tagaattagg cttgcagact atggcctcga tgggcatgct ggctactctg
      301 caaaaggtga gggagatacc ccgaagggct acatgggcct gtacgcgact cagatctttc
      361 cccgactgat ggattgggtt atggctcgac cggcattcgg gcaactccga gaagcattgt
      421 tgcagtcggc ttctggggag atactggaga tcggattcgg aacgggcctc aaccttcgtc
      481 attatccgac tactatcact cgccttttca tcgtcgatcc ggcgattctg cttccagcac
      541 gggtcgcccg ccgcatggct gccgcgccgt atccgatcca aaccatccac gtaccggcgg
      601 aggacctgcc gtttcctgag cgacaattcg acaccgtcgt cagcacctgg acgttctgca
      661 cgattcctga tccggtgctc gcgctgcaag aagtcgggcg tgtcctcaaa ccgggaggga
      721 aatttttatt cttggaacat ggccggagcg acgaccgaaa aatcgccgca tggcaggacc
      781 ggctcaatcc agtccagaag gtgatcggtt gcgggtgcaa cctcaaccgg ccgattgatc
      841 ggttagtcac tcaagccggc ctgacgatcg ttcaccttga tcgattcagc atgcaggatg
      901 tgccaagatt gggtggtgag atgtaccgag gcacggcaac cagacaaagt tagacaggcg
      961 gtttgccgtt aagcagaggc gcaaatccgc tgatggagag aaactgtggg agcagagtcg
     1021 ccggtaattg cgaactcgac ttcgcgctgt gaaccaggtg agccaccccg aactctttcg
     1081 cagccatcag acagccttca tcgtcatcca tgaacaggga acgcgacgga tcgaaaccca
     1141 gcaaacgctg acagttcggc caatactccg gtcgcatctt gagagagccg acctcaaagg
     1201 catccacaat ccggttgacg tagcgatcca gaccggtctt ggcgactttg accgacacac
     1261 ccgtagaatg ggcgttcgtc acgatggtca cacgcttctc gagctgctga agccggcgca
     1321 agaattcctc ggcgccgggg agaaaaccga tcatgtgatc gagttccttg tgcatcgcca
     1381 ccacgtcgat gccgacccgc ttcgtccagt agtccaggtc cgtccaagct aactcgcctt
     1441 cgaccgaccg atacatcgcc atcaggcgat cgcgagcttc ctcaaacggg agagcgtgca
     1501 gcaccgcata ccgacgcggc agctcttcct cgaaaaagaa gttgtcgaaa tggcgatcga
     1561 gcaacgtacc gtccatatcg agcagcacat cgtcgatctg cgtccagtcg gggaagtgct
     1621 gaggactgag gactgaggac tgcaccatgg gcaaaagtat atcggaggtc gaacgggaag
     1681 gcgagacggt tgtctttgag aaaaacccta tgttaccgtc cggcatcttc ctcttggatc
     1741 gtgatcgctt cacatgtctt cacactcagc actcagtcct cagcactcag cacttccgct
     1801 ggtcgccatc atcggacggc cgaacgtggg gaaatcgaca ttgttcaaca agatcttggg
     1861 cgcgaagatc gccattgtgg acgatgtccc cggtgtcaca cgcgaccgca actatgccga
     1921 tgccgcctat cgcaaccgaa agtttcggct ggttgatacg gggggactgg atttatcttc
     1981 ctccgacagc atgttgtccc tgattcgacg acagtcggaa ctggcgattg ctgaagcgga
     2041 tatcctcatc gtgctcttgg acggtcgctc aggattaacc cccccggacc atgaagtggt
     2101 gcggctgctg cgcggagtga cgaagcccct gttctatgcg atcaataaga tcgacacgcc
     2161 gaagtccgag cccttactgg ccgacttcta ccgattgggc accgaccagc tctaccctgt
     2221 gtccgccgag catggcctcg gtgtcgctga gctgttggac gccatgtacc cgcttcttcc
     2281 gtcggcggac gagtccgacg agctgcaaca actccctcgc gtggccatgg tgggacgacc
     2341 gaacgtcggc aaatccaccc tcgtgaatac gctgctggga gaggaacggg tcgtggtcag
     2401 cgatgttccg ggaacaacac gagatccgat cgactcactg gtcacgcacc acgatcaacg
     2461 ctatatcttc accgacacgg cgggcatcag gcggcgcggc aaggtcgacc gcggggtgga
     2521 gggctacagc gtcctccgat cgcttcgtgc catcggtcag tccgacatcg ctgtcctc
//
LOCUS       DATNDD010000335         3852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2143038, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000335 DATNDD010000000
VERSION     DATNDD010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143038"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..681)
                     /locus_tag="VN647_08070"
     CDS             complement(325..681)
                     /locus_tag="VN647_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWG97023.1"
                     /translation="MSERLHHDSVFSLEEALARVDDDRETFQMMIELFMEHGPKDLAD
                     AQAAVGAGNAAGVARSSHRLKGAILQFCAPAALQACKALEESAKAGNLAHGERLYAAL
                     ELEFHRLLAALRQARD"
     gene            complement(674..1897)
                     /locus_tag="VN647_08075"
     CDS             complement(674..1897)
                     /locus_tag="VN647_08075"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWG97024.1"
                     /translation="MPIPAAVTTFRNAAEDGLRTVLVVDDEPTARMALASRLKRLGYR
                     VIEAGDGKAGLEALRRERPDLTILDWMMPEMDGPSFCEQVRQDPELLSSQILMMTSHD
                     RPEQIAEGLARGADDFLSKAASKYEITARVQAGMRTATLIQRLEDVTEQIRRKQEVLE
                     RELQSAAHYVESLLPAPGTLLPGVQMVHAYRPSLGLGGDLFNVVPWSEDVMGLYLLDA
                     SGHGVSSALRSASFSTFLRRGNLLHHVGSNDPGAILTEANKQFPLTEDGNYFTIIFAR
                     LDVRARTLSYATAGHNGALLHRRSGEVRWMTKPNLPLGFDPSTTYLMEELPIGSDDRL
                     YLLSDGLYEVPASNGELWGQDRLEQTVRTFGHRPLAEVVSGTIAEAIRWQGHEHFPDD
                     VAMMGVELTEMVSHE"
     gene            complement(2072..2422)
                     /locus_tag="VN647_08080"
     CDS             complement(2072..2422)
                     /locus_tag="VN647_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWG97025.1"
                     /translation="MAMHTKERPVPNGVVLEMTGDLTYANREQFKTAVETIRQKGCRH
                     LILNMAEVRFVDSSGLGLLALVSQNFKLSQGKVSMLKPQSYVREIMSLANIQKLIPVY
                     DNEQDALAGHQRAA"
     gene            complement(2471..2803)
                     /locus_tag="VN647_08085"
     CDS             complement(2471..2803)
                     /locus_tag="VN647_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWG97026.1"
                     /translation="MTRRVWRDATVIQIDDVFTHRNRKDFSSAVSTFREAGGKHLIVN
                     LREVTFIDSAALGLLALTSQQLAVDECRLSLVDPQGTVKRILEMANIDKMIAVFPTEE
                     AAAEARAA"
     gene            complement(2823..>3852)
                     /locus_tag="VN647_08090"
     CDS             complement(2823..>3852)
                     /locus_tag="VN647_08090"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HWG97027.1"
                     /translation="LMRQVLAMLLAKDPQIEVVGSAPDPYIAREKIKALNPDVLTLDV
                     EMPKMDGLTFLEKLMRGHPMPVVMVSSLTEVGCQTTLRALELGAVDFITKPKIDLREG
                     MEEIAQDLIVKVKAAAVARVRSKEIGGNGHQKPFTSHPSPLPSGSAMIKTTDTIIAIG
                     SSTGGTEAVKEILERLPPHTPPILITQHMPERFTKTWADRLNKLCRISVKEAQDGDSV
                     LPGHALVAPGNFHMTLVRSGARYTVQINQDSPVNRHRPSVDVMFSSVARYAGANAIGV
                     ILTGMGNDGAKEMLAMKQAGAFTIAQDETSCVVFGMPKEAIKAGAVDKVLPLNDIAGA
                     ILTHVSSR"
BASE COUNT          827 a         1178 c         1017 g          830 t
ORIGIN      
        1 cgaaaaccct ctcccctgca actgttcagc aatacgcgcc tgggtgtcgg gacagggatc
       61 gatgaccaag agatgatcgg acgtcgtgag tgtattcatg cggccatccc cttaacccac
      121 attattcatg acagttcgtg acgaaaccgc caagacgcca agcgagacgg gcacgcagag
      181 ccctgagaga ccaaggcata cttgaaacag tatgtcgcgg tcatgaaggg cgagcctgcc
      241 cgtcaggccg caggtaaccc gcggcctggc ttgtcgtagc ggcatatcgg cggttgcagt
      301 agaagtgctc atgaataatg tgggttagtc gcgcgcctga cggagtgcgg ccagaagacg
      361 gtggaattcc agttccagcg cggcatagag ccgctcccca tgcgccaagt ttcctgcttt
      421 cgccgactct tccaatgctt tgcaggcttg gagggcggcc ggagcgcaga actgtaatat
      481 cgcgcccttc agccggtgac tggaccgtgc cacacctgcc gcattccctg cacccacagc
      541 agcctgagca tccgccaagt ccttcggacc atgctccatg aataattcga tcatcatctg
      601 gaacgtctct cggtcatcat cgacacgggc gagagcttcc tcgagactaa aaacggagtc
      661 atggtgcagg cgctcactca tgagaaacca tctcggttaa ttcaactccc atcatcgcca
      721 catcatcggg aaagtgttcg tgcccctgcc atcgaatcgc ctcggcaatg gtgccggaga
      781 ccacttccgc caacgggcga tggccgaacg tgcgaaccgt ctgttccagc cgatcttgtc
      841 cccagagctc gccgttcgaa gccggcactt cgtacagacc gtcgcttagc aggtataacc
      901 gatcatccga tccgatcggg agctcctcca tgaggtaggt cgtcgacgga tcaaaaccca
      961 gcggcagatt cggcttggtc atccatcgga cctcaccgga ccgacgatga agaagcgctc
     1021 cattgtgtcc ggccgttgca taggacagcg tacgagctcg gacatcgagt ctggcaaaga
     1081 taatcgtaaa ataattcccg tcttccgtca gaggaaactg cttattcgct tccgtcaaga
     1141 tcgcacccgg atcgttggac ccgacatggt gcagcaaatt gcctctgcgc aggaaggttg
     1201 aaaacgaggc agaccgcagt gccgatgaca ccccatggcc ggacgcatcc agcaaataca
     1261 agcccatcac gtcctcactc cacggcacga cattgaaaag atcgccgccc aaaccgagcg
     1321 acggccgata ggcatggacc atttgtacgc ccggcaagag cgtccccggc gccgggagca
     1381 acgactccac ataatgcgcg gcggactgga gctcccgttc gaggacttcc tgtttccttc
     1441 ggatttgctc cgtcacatcc tcgagccttt ggatcaaggt ggccgtgcgc atgccggctt
     1501 gcacgcgggc cgtgatctca tacttgctgg cggccttgct gagaaagtca tcggcgcccc
     1561 tggccaggcc ttcggcgatc tgttcaggcc ggtcatgact cgtcatcatg aggatttgac
     1621 tcgacagcag ctcagggtcc tgacgaacct gctcgcagaa agacggtccg tccatttccg
     1681 gcatcatcca atccaagata gtcagatcgg gccgctcgcg tcggagcgct tccaacccgg
     1741 cctttccatc gccggcttca atcacacgat agcccagccg tttgagtcga gacgccaagg
     1801 ccatccgtgc ggtgggctca tcgtccacga ccaaaacagt ccgcaatccg tcttctgcgg
     1861 cattgcggaa ggttgtcact gcggctggaa tcggcatggc atgagactcg atgagaacac
     1921 ctataagcgg ttcaatcgac gaacacgccc tctatccgat caggtcgtga gaaaacacta
     1981 tttagagaga tcctggatca agattccagg gatattaccg actcaaacag cagatgcgag
     2041 aacgccgctg acggcatttt tcaacatcct gctaggctgc gcgctggtgt ccggccaacg
     2101 catcctgctc attgtcatag accgggatca gtttctgaat attggccagg ctcatgattt
     2161 cccgcacgta actttgcggc ttcaacatgc tcacttttcc ttggctcaat ttaaagttct
     2221 gagaaacgag cgccagcaag ccgagcccgg aactgtccac aaatcggact tcggccatgt
     2281 tcaagatcag atgccgacag cccttctgtc gaatcgtttc cacggccgtt ttgaattgtt
     2341 cacgattcgc gtacgtgaga tctcccgtca tctcaagaac cacaccgttc gggactggac
     2401 gttctttcgt gtgcatcgcc atgagtacct ccttcgttca gaccaatcga gatcggctcg
     2461 tctccaccgt tcacgcagcc cgagcttcgg ccgccgcttc ctccgtcgga aacactgcaa
     2521 tcatcttatc gatattggcc atctccagaa tccgcttcac ggttccttgc ggatccacga
     2581 gactgagcct gcactcatca accgcaagct gttgagaggt caaggccagc aggcccaagg
     2641 cggcactgtc gataaaggtc acctcgcgca gattgacgat gagatgcttc cctcccgcct
     2701 ctctaaaagt cgacaccgcg gaagagaaat ccttgcggtt gcggtgcgtg aagacatcat
     2761 cgatttgtat cacggtcgcg tcccgccaaa cccgtcgggt catgggcata aatcctccgg
     2821 attcatcggc tgctcacgtg ggtcagtatg gcgccggcga tatcgttcag cggcaaaact
     2881 ttatccaccg ccccggcttt gatcgcttct ttcggcatgc cgaacacgac gcagctcgtt
     2941 tcatcttgag cgatcgtgaa ggcaccggct tgcttcatcg ccaacatttc tttggcgccg
     3001 tcgtttccca ttcccgtgag aatcactccg atagcgttgg cgccggcata acgggccacg
     3061 gacgaaaaca tcacatccac ggaaggccgg tggcggttga ccggggaatc ctggttgatc
     3121 tgcaccgtgt atcgagcccc gctcctgacc aacgtcatgt gaaagttgcc gggggcgacc
     3181 aatgcatgtc ccggcaagac actgtctccg tcctgcgctt ccttgacgga gattcgacaa
     3241 agcttgttca gccggtctgc ccaagtcttc gtaaaccgct ccggcatatg ttgcgtgatc
     3301 aggatgggcg gggtatgcgg ggggagcctt tccaatatct ccttcacagc ctccgttcca
     3361 ccggtcgaag aaccgatggc gatgatggta tcagtcgtct tgatcatggc gcttccggag
     3421 gggagaggtg acgggtgaga ggtgaacggc ttctgatgac cgttcccccc tatctcctta
     3481 ctccttaccc gagccaccgc agcggctttg actttgacga tgagatcttg ggcgatctct
     3541 tccatccctt cccgaaggtc gatcttcggc ttggtaataa aatctaccgc tccgagttcc
     3601 agtgcgcgca atgtagtttg acagccgacc tccgtcagcg aactcaccat caccaccggc
     3661 atggggtgcc cacgcatcag cttctccaaa aatgtgagtc cgtccatctt gggcatttcc
     3721 acatcgagag tcagcacgtc tgggttcagc gccttgattt tttctcgcgc gatatacgga
     3781 tcgggagccg atccgacgac ttcaatttga ggatctttcg cgagcaacat ggcgagcact
     3841 tgccgcatca ac
//
LOCUS       DATNDD010000336         7574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_704233, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000336 DATNDD010000000
VERSION     DATNDD010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704233"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN647_08095"
     CDS             <1..275
                     /locus_tag="VN647_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97028.1"
                     /translation="GGTGEPVKRKGWSQFIDATAMGDDTNRYDEVGCVFCIQHTPVTH
                     PKLVLPCESSGQGLGLCLVTILTQPLDFCRNSFGIESEKVFKPLAV"
     gene            649..1239
                     /locus_tag="VN647_08100"
     CDS             649..1239
                     /locus_tag="VN647_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97029.1"
                     /translation="MKDTLLNYSEWTPKREELERIYDARLSYRAVFMQKGEDIPGMSA
                     TPSAERYAKVILLLERENRINEFDQVVGRLRSTYDHEMKRLGLNSSPCGKDNSWISWD
                     VAKQLYYLFNGDQPANNIRYRIADPLRDKRICDEIVCRHIGGELWVSGIPEPWAHHAE
                     VRSLLGPECESSVHQGHPASWALARVGAIVRSSQLT"
     gene            1540..3210
                     /locus_tag="VN647_08105"
     CDS             1540..3210
                     /locus_tag="VN647_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWG97030.1"
                     /translation="MVNPDVSSPRHASAAPQELALLDTEEEPSTTEGRDQYFTPGHYA
                     SPGGAQAGGGQTADSGCASYEPGEVHASKQEPGILSESVLSTGTDALVLADFGVGWRH
                     VKSSTANHSLIRAWQRVLTDNPLATVEVTGFSDCVGSKTDNETLRLDRAKRVASLLGS
                     NLRLSQLTVKAAPHGTFLTDNSSRSNRAMNRSVKVRIRYPSSQPQLGLLPDENLCGGR
                     KCFSDEDIYPPEKAKGDTAASGLVSSPRGFKYSKDLKKGTMKWTLKIAVDQDKPGPAA
                     RIQIAFTPKPSYRSHTVTFLQTVVDTKADTRFAPPERTFVDIRPDVEEVEPFYGVDWN
                     PATNTWQAESIKTPVPQGFKNQPSTNTDLSTYLFDEPWLMYAGQAKIFESVVVIPETG
                     EVLGSLRWGVTKTQLLGGGLDDCTDLPSHGFDVAMQNYYSMPKGSDPQSPGRFDTIID
                     GYDFGVYELSEENKKKVDPIIDAFLKGSINSPTMRIAVSGFADAGEHDDPEFTSLMRA
                     LLVQRYMMSKGVPQDKFDVYSFGAAWARYLVSPGENRNRRVQIRTYYK"
     gene            3923..4822
                     /locus_tag="VN647_08110"
     CDS             3923..4822
                     /locus_tag="VN647_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HWG97031.1"
                     /translation="MKSGTTRGASYRLAGRADQPIRLTRSLATRGCDEDRIWDEIEVR
                     LQLKRALRPNVLAIMRYAFTEMLNNAVEHSQSDRCTIQVVLGPSQISFDIRDPGIGVF
                     HSIASKFHLPDEETALVELLKGKTTTMPQAHTGEGLFFTSRVGDTFSLKSHRIQIEWN
                     RAKEDVFISQSRFTRGTLVQFSVRRGARQTLDHVFGEFAPQKYDFQFQKTEVRVKLLQ
                     PDYVSRSEARRLLANLEKFREIIFDFRDVRSVGQGFADEVFRVFADRNPGIVLRPENA
                     GPAVMAMVRHVSREASLNRQPQP"
     gene            complement(4875..6509)
                     /locus_tag="VN647_08115"
     CDS             complement(4875..6509)
                     /locus_tag="VN647_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97032.1"
                     /translation="MFISVAHWIDRNILSLGREMRLSYLPPLMVYVAYGISGLTGIVG
                     TFFVKDYLGLSAAFLAALGFWAGIPWALKMPIGHTVDLVWRWKSWLVGLGGGLLTISL
                     GIMALLIGNREAMTAIFPAEVWYVLSVLLAPIGYVIQDVVADAMTVEAVPRVDHQGHP
                     FDDQTCKLMHTTMQTLGRVAIVGGGIFVALINVYVFSGTEGLPQAELIRLYKKIYLMA
                     MVIPVVSVAGVGFAWWLKRRQMQRLIQKGFSLEQVKKMVDVRDVENEPTKPNWWILGG
                     SSAFVLFTLTVGLGGFPYREEIVFAGSMSIVLFLMSRLVQELEPDKRITLVGTAAVIF
                     ALRAIPGPGPGSTWWMIDDLKFDQQFLSVLSLIGGMLALMGMFAFRRFMAERSIMYVV
                     GFLTVVGTILSLPIVGMYYGLHEWTVKVTGGVVDARFIALVDTALESPLVQISMIPML
                     AWIANSAPANLKATFFAVMASFTNLALSFSQLGTKYLNEIFIVTREVRDQASGVLQTP
                     ADYSQLGDLLIMQLVIGLALPFSAILFAKLTKFKSA"
     gene            complement(6512..7396)
                     /locus_tag="VN647_08120"
     CDS             complement(6512..7396)
                     /locus_tag="VN647_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWG97033.1"
                     /translation="MADRNKRLDSNVPGNFYVDATCINCDTCRQLAPLSFEEVGDYSA
                     VNRQPEDEKQIHQAYQALLACPVGSIGAEHGDNARLHTAMASFPLQIENGVSYCGFNS
                     EKSFGANSFFIEHPDGNWLIDSPRYIKHLVEAFERRGGIAHIFLTHKDDVADADKYAA
                     HFGAERIIHQADKEAAPAAERVMGGEEAVRVGSEFEIIPVPGHTAGCIALLYRERFLF
                     TGDHLWWNPHIKSLEAPTRLIWRRWTLIESIRKLLDYPFEWVLAGHGDRVHLSSTEMR
                     AQLSALVKRRETKGGVLR"
BASE COUNT         1867 a         2029 c         2019 g         1659 t
ORIGIN      
        1 aaggcggaac tggagagcca gtgaagagga aaggctggtc acaatttata gacgctacgg
       61 cgatgggcga tgacacgaac cgttatgacg aggttggctg tgtcttttgt atacagcaca
      121 cgccagtcac ccacccgaag cttgtactgc cctgcgaatc gtccggtcaa ggtctcgggc
      181 tgtgtttggt caccatactc actcagccat tggatttttg ccgtaattct tttggtatcg
      241 agtcggaaaa ggtcttcaag ccgcttgcgg tgtgaaatca acagagtacg tcaacggaga
      301 ttaagccttt tctttacctc ctcaaagggg atgcgctctg ttgattgcag ggaggacgac
      361 agccgagttt ctatttcttg gcttaactca agcccggtat cgggtccgcg agcagattgt
      421 gtagctcttc atggatggtt tttttgatta aggtacggaa ttcttcgact gttaggtcgg
      481 cggccttcat caaggtcctc ccttgtcgga aggatatcga tcacggccag ggaaagtcaa
      541 tgattgagca ggaaaatgag acgggctcct gttccaggcg atcggacgag tcacggcttt
      601 atcatcagcg ataagcgcca accctgaaat cgataacgga ggggcaagat gaaagataca
      661 cttttaaatt actcagagtg gactcccaag cgagaggagc tggaacgtat ctacgatgcg
      721 cggctgtcat atcgagctgt gttcatgcag aaaggcgagg acattccagg tatgtctgcg
      781 acgccgagtg ctgaacgtta cgcaaaagtt atactccttc tggagcgcga gaatcggatt
      841 aacgaattcg atcaggtcgt tggacgtctc cgctccacct atgaccatga aatgaaacgt
      901 ctggggctca attcaagtcc gtgtgggaaa gacaattcgt ggatatcttg ggatgttgcg
      961 aagcagctct actacctttt caatggtgat cagccggcaa acaacatccg atatcgcata
     1021 gcggacccgc tgagggacaa acgaatttgc gacgaaatcg tttgccgcca tatcggaggg
     1081 gagttgtggg tttcgggcat tcctgagccg tgggcacacc acgctgaagt gcgtagtctg
     1141 ctgggaccgg aatgcgagag ttcagtgcac cagggtcatc cagcctcctg ggctcttgcg
     1201 agagttggcg caatcgttag atcttctcaa ttgacataaa atcaaaattg cggtagcggg
     1261 gatgttagtg gggtgttgcg gcgctgatcc gcagcgactt tgaacaattg tcggagctta
     1321 gcgcggacgg taacatagag tttattgaaa aggacgatgg caaagttagg tcacaccatt
     1381 tagataaaag gaagcagaat ggctcgaatc cttacacgat cggccacgca gagtcgttct
     1441 ggtacagaag tcggctcctc gcttgaaccg acccaccaca agcaggacga cccacttctt
     1501 catctacagc aaacggtcgg aaatcgcacg gttcagcgct tggtcaaccc tgatgtctct
     1561 tcacctagac atgcctccgc tgctcctcaa gaactcgcgc ttctggatac ggaggaagaa
     1621 ccgtcaacca cggaaggcag agatcagtat ttcacgccgg gccactatgc gagccctggc
     1681 ggtgcacaag caggtggcgg tcaaacagca gatagcggtt gcgcgtcgta tgagccagga
     1741 gaggtccatg cctccaaaca ggaaccgggg attttatctg aatcggtttt atccactggc
     1801 acagacgccc tcgtccttgc cgattttggc gtgggttggc gtcacgtcaa gtcatcgact
     1861 gccaatcatt cgttaattcg ggcgtggcaa cgcgtgctta cagacaaccc cttggccacg
     1921 gtggaagtca ctggtttcag cgattgtgtc ggatccaaga ccgataatga aacgctgcgc
     1981 cttgatcgtg cgaagcgtgt ggcatcgttg cttggctcaa acctacgtct ctctcaatta
     2041 accgtgaagg ctgccccgca cggtacgttc ctcacggata actcaagccg ctcgaaccgc
     2101 gccatgaacc ggagcgtgaa ggtccgtatc cgttatcctt cttcgcagcc acagcttggc
     2161 ttgcttccag atgaaaacct gtgtggcgga cgaaaatgtt tttcagatga agatatctat
     2221 cctcctgaaa aggcgaaagg cgatactgcc gcttccggct tagtctctag cccgagaggg
     2281 ttcaaatact cgaaggacct caagaagggc acgatgaaat ggaccctaaa aattgctgtg
     2341 gatcaagaca aaccagggcc tgcggcgcgt atccagatcg catttactcc aaagccctcc
     2401 tatcgctctc acaccgtcac cttcctgcag acggtcgtgg atacaaaagc cgatacgcgc
     2461 tttgcaccac cagaacggac attcgtggat atacgccccg acgttgaaga ggtagagcca
     2521 ttttatggtg tcgattggaa tcctgccaca aatacatggc aagctgagag catcaagact
     2581 ccggtcccac aagggtttaa gaatcaaccc agcaccaata cagacctttc cacctacctt
     2641 ttcgacgagc cttggcttat gtatgcaggg caagcaaaga tctttgagtc cgtggtcgtg
     2701 attccggaaa ctggggaggt tctgggatca ctcagatggg gagtgaccaa gacccagctc
     2761 cttggagggg gtctcgacga ctgcacagac ctgccttcac atggttttga cgtcgcgatg
     2821 cagaactact actcgatgcc caagggctct gatccccaaa gccccggtcg ctttgatacc
     2881 atcattgatg ggtatgattt cggcgtttat gagttaagcg aggaaaataa gaaaaaagtg
     2941 gaccctatca tcgatgcatt cctcaaggga tctatcaata gccccaccat gcgaatcgcg
     3001 gtcagcggat tcgcggatgc gggggaacat gacgatccag aatttacctc gttgatgcgg
     3061 gcccttcttg ttcaacgtta catgatgagc aaaggggttc ctcaagataa gttcgacgtc
     3121 tatagcttcg gcgcagcctg ggcacgttat ttagtaagtc ctggagaaaa tcgcaaccga
     3181 cgagttcaga ttcgcaccta ctacaagtag gacgatggac tgctgtaact ctcatcctga
     3241 atcaagccac ttaaggtgac gtcacgcgca ttgatgtact tgagaggcgc ttttgcggcg
     3301 catgccggtg actggttcaa atacattctc catcgcagcg cgcatgaagt tcaatctcga
     3361 acgagcccct tcaacagaag ccattcttca tgagctgcca tgtcatttca atgcgcaagt
     3421 ggacctgccg cagagcgaca accttcagcg aagaatgcga agagcgaaaa aagcaagaaa
     3481 gtgctgggaa gccgatgtca tacgatactg tatcgtttca cgcctctttt cgtctctgca
     3541 accgctgcag caaggactca cgcgcccttg aacttggtga gctgggcaaa caagttcacg
     3601 gagaagggaa gagccaggcc gatcaccaat tacgtgatca agagacagcc gctagacaac
     3661 gacaagattg gaatcgtggc gcagtgccaa agcgaggaat acacgaaacc cgttgaacag
     3721 gggaccttga caattacaat gttcttacat tgacaattga ttgacaatat gacaccgttt
     3781 cgccatgcat cctaagcggc agatccttgc ttttctatct cgtcgaagct ctgcttcggg
     3841 aggagagcta cgagagcgcc tcaagctcag ccggcaagcc ttaagtctcc atcttaaaaa
     3901 cttgcttgcg gagcacaaaa tcgtgaaatc gggaacaacc cgaggagcca gctataggtt
     3961 ggccggccga gcagatcaac ctattagact gactcgttcg ctcgctacga gaggctgcga
     4021 tgaagatcga atttgggatg aaatcgaggt gcgtctccag ctcaagcgag cgcttcgccc
     4081 caatgtattg gcgatcatgc gttatgcttt cacggaaatg ctcaacaatg ccgttgaaca
     4141 ttcacaatct gatcgatgta ctattcaagt tgtgctaggg ccaagccaga tatccttcga
     4201 tattcgcgat ccgggaatcg gcgtatttca ttccattgcg tcgaaattcc accttccgga
     4261 tgaagagaca gcgctcgttg agcttctcaa aggtaagacg accaccatgc ctcaagctca
     4321 caccggcgaa ggactttttt tcacatcccg ggtcggtgat acattttcct tgaagtccca
     4381 ccggatacaa attgaatgga atcgggccaa ggaggacgtg tttatttcac agtcacgttt
     4441 cacgcgtgga accctcgtac agttttcggt tcggcgtggt gcccgccaga ctctcgacca
     4501 tgtgttcggt gaattcgctc ctcaaaagta tgatttccag tttcaaaaga ctgaagtacg
     4561 ggtcaagctt ctgcagcctg actatgtttc acgctctgag gcaagacgcc ttttggctaa
     4621 cttggagaag ttccgtgaga tcatctttga ttttcgagac gtgcgctcgg tgggccaagg
     4681 gttcgcagac gaagtgtttc gcgtgtttgc cgatcgcaac cccggtattg tgcttcgccc
     4741 cgaaaatgcc ggtcccgccg tcatggccat ggtcaggcat gtgagccgag aagcttccct
     4801 caaccggcag ccgcaaccgt aatcagtcta ggggaaagaa cgccggcgga gtcgatctcg
     4861 atagccgcac aaggtcacgc gctcttgaac ttggtgagtt tcgcaaacag gatggcggag
     4921 aagggaagag ccaggccgat caccaattgc atgatcaaga gatcgccgag ttggctgtaa
     4981 tcggctggag tctggagaac accgctcgct tgatcccgta cctccctggt gacgatgaag
     5041 atctcgttga gatacttggt accgagctgg ctgaaagaaa gggcgagatt ggtaaaggag
     5101 gccatcacgg caaagaaggt ggctttcaga ttggccggag cggaattcgc gatccatgcc
     5161 aacatcggaa tcatggagat ctgcacaagc ggcgattcca gtgccgtatc gaccagagcg
     5221 atgaacctgg catcgacgac tccgccggtc accttcaccg tccattcatg caatccgtag
     5281 tacatgccca cgatcggtag agaaaggatc gtcccgacga cggtcaaaaa tccgaccaca
     5341 tacatgatcg accgttcggc cataaagcgc cgaaaggcga acatgcccat caaggctaac
     5401 atgccgccga tcaaggagag gacggacagg aactgttgat cgaacttgag gtcatcgatc
     5461 atccaccagg tcgacccagg gccgggtcct ggaatcgcac gcaaggcgaa gatcaccgcc
     5521 gcggtgccca ccaaggtgat ccgtttatca ggctccagtt cctgaacgag tctcgacatc
     5581 aagaacagca cgatgctcat cgaaccggcg aacacgatct cctcccgata gggaaatcca
     5641 cccaatccca ccgtcaacgt gaagaggaca aaagctgaac ttccgccgag aatccaccaa
     5701 ttcggtttcg tcggctcgtt ctccacgtca cgcacatcca ccatcttctt gacctgctcc
     5761 agtgagaatc ctttctgaat gagtcgctgc atctgccgcc gcttgagcca ccatgcaaat
     5821 ccgactcctg ccactgagac aaccgggatc accatagcca tcagatagat cttcttgtac
     5881 aaccgaatga gttcggcttg aggcaatcct tccgtcccac tgaagacata cacgttaatg
     5941 agcgccacga agatcccacc gccgactatc gcaacacgtc cgagtgtctg catcgtggta
     6001 tgcatcagct tgcatgtttg atcatcgaat ggatgcccct gatggtcgac acggggaacc
     6061 gcttcgacgg tcatagcgtc cgcaacgacg tcttgaatga catacccgat aggggcaagc
     6121 agaacgctga ggacatacca gacttcagcg ggaaagatcg ccgtcatcgc ttcgcggtta
     6181 ccgatcaaca gggccatgat accgaggctg atcgtcaaca gccctccgcc caacccaacg
     6241 agccagctct tccatcgcca tacgagatcg accgtgtggc cgatcggcat cttcaaggcc
     6301 cagggaatcc cggcccaaaa tccaagcgcg gcgagaaatg cggcggagag tccgaggtag
     6361 tctttaacga agaaagtccc gacgatgccg gtgagacctg aaataccgta ggcgacatag
     6421 accatgagcg gcggcaaata ggacaaccgc atctcgcggc cgagagagag aatattgcga
     6481 tcaatccaat gagcgacgga tatgaacatc gctatcgcaa tacgccgccc tttgtttccc
     6541 gacgctttac caatgccgag agttgcgccc gcatctcggt cgaggagaga tgcactcgat
     6601 cgccgtggcc tgccaggacc cattcaaacg ggtaatcgag gagtttccgg atggactcga
     6661 tcaaggtcca tcttctccaa atgagacggg taggagcttc caacgacttg atgtgtggat
     6721 tccaccagag gtgatcgccg gtgaagagaa atcgctcgcg gtagagcagg gctatgcatc
     6781 cggcagtgtg ccccggcacg ggaatgattt cgaactctga tccgacccga accgcttcct
     6841 cacctcccat cactcgttct gcagccggag cggcctcttt gtccgcctga tggatgatgc
     6901 gctccgcacc gaaatgcgcg gcatatttat ccgcatccgc cacatcatct ttgtgggtca
     6961 gaaagatatg ggcgatccct ccccgccgct caaaggcttc aacgagatgc ttgatgtacc
     7021 ggggagagtc gatcaaccag ttgccgtccg gatgttcaat gaaaaaacta tttgctccga
     7081 acgatttctc cgaattgaaa ccgcaataac tcaccccgtt ttcgatctgg aggggaaagc
     7141 tcgccatggc tgtgtgcaat cgtgcgttat cgccgtgttc agcgccgatc gagccgacgg
     7201 gacaggcaag caatgcctgg taggcctgat gaatctgctt ttcatcctcc ggctggcggt
     7261 tgaccgccga atagtcgccg acttcttcaa agctcagcgg cgccaattgg cgacaggtgt
     7321 cgcaattgat gcaggtggca tccacgtaga agttgccggg aacgttggaa tcgaggcgtt
     7381 tgtttcggtc ggccatcaga acacttccat agcccgtttt tctcgatcgg cggcacgcca
     7441 gaggtaccag gcggcggccg tacgataggg tctccatcgc tcgccatacc gtaacatctc
     7501 tttcggcatc ggcatcgtcg accggcgata ggcgatgcgg aagccgttgc gcaggccgaa
     7561 atcatcgacc ggca
//
LOCUS       DATNDD010000337         3685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2144595, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000337 DATNDD010000000
VERSION     DATNDD010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144595"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VN647_08125"
     CDS             complement(<1..360)
                     /locus_tag="VN647_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWG97034.1"
                     /translation="MRGKRLVMTVCSLIAVAVWGTAGWAAPEADPLKPRVPEAERADV
                     RKAKSPLTVTPDIIAKGKEIYEGKGTCVNCHGMTGAGDGPGGMLLSPGPRNFTNCKFH
                     KKRNDGELHWIIKNGSPG"
     gene            complement(638..1081)
                     /locus_tag="VN647_08130"
     CDS             complement(638..1081)
                     /locus_tag="VN647_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HWG97035.1"
                     /translation="MRFSKQIATVAIAVAATWTLSSATSFAAERSLYERLGGQGAIQA
                     VVTKFIGNVGADKRINGYFATTDLKNLNKLLVEQVCAASGGPCTYSGRDMKTTHKGMK
                     VTTAAFNALVEDLVSALDTFNVPKKEKDELLGVLGPMKKDIVEVP"
     gene            complement(1144..2091)
                     /locus_tag="VN647_08135"
     CDS             complement(1144..2091)
                     /locus_tag="VN647_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWG97036.1"
                     /translation="MQRFRVLTLTLGLGVLLGASGCITMPGSAGAKVHDVTFTATETE
                     IVIDGTGTKYKAWTFNGQMPGPVVRVTEGDTVNFTLINPETNAQGHSMDFHAAEIDFL
                     KNYREIRPGETIKYTFVAKKPGVFFYHCGAPPMIQHIGRGMFGAIIVDPKNANAWPKA
                     DREFVLVQSELWKNPDNVQAMFDRKFDHTIFNGGVFKYHPFFPGSEPLEVKVGERVRI
                     YFVNAGPNEFSSLHTIAEIWDNVYESGNPANKFTGVQTYVVGPGSAATFDMIVDEPGA
                     YPIVTHSLTGALRGAIAVVVANQNPKKYDSLMPLTPWNP"
     gene            complement(2243..3292)
                     /gene="queG"
                     /locus_tag="VN647_08140"
     CDS             complement(2243..3292)
                     /gene="queG"
                     /locus_tag="VN647_08140"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWG97037.1"
                     /translation="MSITAAIKQEARALGFDAVGITHVSDEPTGSTTGDQQNGPLSHS
                     EAAVSQRLFDRLTEWLRRSYHGTMAWIERAPNKRADPGQVLPGCRSIISLGINYLTEH
                     RANEQPGYGRIARYAWGRDYHKLFDSRLKQLERLIHEMAPGAKTRSYCDTGPIMEKAW
                     AEQAGLGWIGKHSNLVSAEYGSWLLLGEVLTTLELEPDEPATDLCGSCSLCIQACPTE
                     AITEPYIVDATRCISYLTIELRGDDTAIPHGLQAGMGNKIFGCDDCLDVCPFNLRAEP
                     TQEHTFQPSSWSLSPDLNELSKLDESTFTTVFQQSPIRRAKHQGLLRNVAIAQRNLTS
                     PASSGVASSKEECSD"
     gene            complement(3307..>3685)
                     /locus_tag="VN647_08145"
     CDS             complement(3307..>3685)
                     /locus_tag="VN647_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97038.1"
                     /translation="IMTIRRMVFGFFVLLLLILLTPALDANAQGIEDSGATTELSCSF
                     SMEKDETGHPCRVPFPQGCRVVSVPRTKQPWTTISKAGRLQCRFDRTETDWKTKIVGT
                     CGRCKSVRCSAHFSVRFDCSAQH"
BASE COUNT          769 a          976 c         1045 g          895 t
ORIGIN      
        1 gccgggactt ccgttcttaa tgatccagtg cagttctccg tcgttccgct tcttgtggaa
       61 cttgcagttc gtgaagtttc gagggccggg cgacaacaac attccgccgg ggccatcgcc
      121 tgctccagtc atgccatgac agttgacgca ggtccccttg ccttcataga tttccttgcc
      181 tttggcgatt atgtcgggtg tcacggtcaa aggactcttt gccttccgta cgtcggctcg
      241 ttcagcttcc gggacacgcg gcttcaatgg atcggcctcg ggagcggccc atcccgccgt
      301 accccatacg gcaactgcaa tcaagctgca cacggtcatg actaaacgtt tccctctcat
      361 gattcctcct cggtgattac cactgatgtt acggccagat ggttattcgc gccgcgtgtt
      421 cggaggcgct tataacggga gatatgataa cacgatatcg ccctaagtgg cagcgtccat
      481 atttcctgga tagtgagcag atcgaaataa agtcggaggc tggtttggaa catttacagg
      541 aaggtattgc gggccggaac tttttgaggt cccggcccgc atgaggctca ttatgggact
      601 tgcagtcatt accactgcac gtcacgcacc ggcatcatca gggaacttcg acgatatctt
      661 tcttcatcgg ccccaggacg ccgagcaact cgtctttttc tttcttcggg acgttgaagg
      721 tatccagcgc actgaccaga tcctctacga gtgcattgaa ggcagcggtg gtgaccttca
      781 tgcccttgtg cgtcgtcttc atgtctcgac ctgagtaggt gcaggggccg ccgcttgccg
      841 cacacacctg ttcgaccaag agtttattga ggttcttgag atcggtggtc gcgaaatagc
      901 cgttaatgcg cttgtctgct cccacattgc cgatgaattt ggtgacaacg gcttgaatgg
      961 cgccttgtcc gcccagccgc tcatagagcg accgctcagc ggcaaaagat gtggcactgc
     1021 tcagtgtcca ggtggctgcc acggcaatgg caacagtcgc tatctgcttc gagaatctca
     1081 tgtcgtctgc tccttttttt gtaagaataa aatgactgca tgacgtttgc accccgctat
     1141 tgactaaggg ttccaggggg tcagtggcat caaactgtca tacttctttg ggttctggtt
     1201 ggctaccacc acggcaatcg caccgcgcaa ggctcccgtt aaggagtggg tcacgatagg
     1261 ataggctccc ggttcatcaa cgatcatatc gaatgtggct gcgctgcccg gaccgaccac
     1321 ataagtctgg acacccgtaa atttgttggc cggattgccg ctttcgtaga cattgtccca
     1381 gatttcggcg attgtatgga gagaagagaa ttcattcggg ccggcattca caaagtaaat
     1441 tcgtacccgc tcaccgactt tgacttccaa cggctccgat cccgggaaga agggatggta
     1501 cttgaagacg ccgccgttga aaatggtgtg atcgaatttc cgatcgaaca tggcctgcac
     1561 attgtccggg ttcttccaca actcggattg caccaggaca aattcccgat cggctttggg
     1621 ccaggcattc gcgttcttcg gatcaacgat gatcgccccg aacatgccgc ggccaatgtg
     1681 ttggatcatc ggtggtgcgc cgcagtgata gaagaacacg ccgggcttct tggctacgaa
     1741 ggtatacttg atcgtctcgc cgggtctgat ttcgcggtag ttcttcagga agtcgatctc
     1801 cgccgcgtga aagtccatcg aatggccttg ggcgttcgtc tcggggttga taagggtgaa
     1861 gttgacggta tcgccctcgg tcactctcac gaccgggccc ggcatttgac cgttaaaggt
     1921 ccaggccttg tacttcgtac cggtaccatc aatgacgatc tccgtttcgg tcgccgtgaa
     1981 cgtgacgtca tggacctttg ctcctgcgga gccgggcatt gtgatacacc cgctggctcc
     2041 caacaggacg ccgagtccca gggttaatgt gagtacacga aacctctgca tagtagcctc
     2101 cctttagatt tttatggaaa agagacgctg caccgatgag cgcggcgaat caggtctttt
     2161 cctctctccg attcggcgga tagtagcgta gcaaggtgta ttagtcaatt tcttgattag
     2221 gggaaattta cattgatcga gatcaatcgg agcactcttc tttcgatgag gcgactccag
     2281 aggatgctgg agaggtcagg ttgcgctgcg cgatggccac gttccggaga aggccctggt
     2341 gttttgcgcg tcgaattggg ctttgttgaa acacagtcgt gaaggtcgat tcatccagtt
     2401 tggaaagctc gttgagatca ggggagaggc tccagcttga aggttgaaaa gtatgttcct
     2461 gagtcggttc ggctcgtaaa ttgaatggac acacgtcgag acaatcgtca cagccgaaga
     2521 tcttgttccc catgcctgct tgtagcccgt gagggatggc ggtgtcatcg ccacgcaatt
     2581 ctatggtgag gtatgaaata caacgagtgg catcgacaat gtagggctct gtgatggctt
     2641 ctgtggggca ggcttgaatg cagagcgagc aactgccgca gagatcggtc gccggttcgt
     2701 cgggttccag ctctaatgtg gtcagcactt ctcccagcag gagccaagaa ccatactcgg
     2761 ccgacacgag attggaatgt tttccgatcc agcccaagcc cgcctgttcg gcccaggcct
     2821 tctccatgat tgggccggta tcgcagtagg aacgggtttt ggcgccgggt gccatctcgt
     2881 gaatcaaccg ctccagttgc ttgagcctgg aatcgaaaag cttgtggtag tcccttcccc
     2941 acgcatatcg cgcaatacgg ccgtagcctg gttgttcgtt ggcacggtgc tcggtgagat
     3001 agttgattcc taacgagatg attgatcggc agccgggaag cacctggcca ggatcggcgc
     3061 gtttattcgg ggcccgttca atccaggcca tggtaccgtg atagcttcgc cggagccatt
     3121 cggtgagacg gtcgaagagc cgttgagata ccgcggcttc ggaatgggaa aggggaccgt
     3181 tctgctggtc gccggtcgtt gatcccgtag gctcatcgga cacgtgcgta atcccaaccg
     3241 catcgaatcc cagcgcacgg gcttcttgtt tgatggctgc cgtaatggac atggggtgca
     3301 aagagctcaa tgctgggcgg aacaatcaaa gcggacactg aagtgggccg aacatcgaac
     3361 cgatttgcat cggccgcagg tgccgacaat ttttgtcttc caatcggtct ccgttctatc
     3421 aaaccgacac tgcagtcgcc cggctttcga gatcgtggtc cagggctgct tggttctggg
     3481 aacggagacg acccggcatc cttgtgggaa tggaacgcgg caggggtgcc cggtttcatc
     3541 tttttccatt gaaaagctgc acgacaactc cgttgtggct ccggaatcct ctatcccttg
     3601 tgcgttggcg tcgagagcgg gagtgagaag tatcaagagc agcaaaacaa aaaagccaaa
     3661 caccatgcgt cgaatcgtca taatc
//
LOCUS       DATNDD010000338         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3069048, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000338 DATNDD010000000
VERSION     DATNDD010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3069048"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VN647_08150"
     CDS             <1..583
                     /locus_tag="VN647_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HWG97039.1"
                     /translation="GAALLGLAIGGAIVLYPARTLPSTTSICLSMDKVADYIHAVIEA
                     DRDSYTRHVVERMQTKGIVVASENWEDMHTLPLPAQFLLESGRHLDKKGIGIQYRLIS
                     LWPINKRNVATTPLQSIALGTIVTQPNHPFTGVTKVGDTRYYEAVYPDLAVTQACIGC
                     HNAHPNSPKRDFKVNDVMGAIVISIQLGPGFPG"
     gene            complement(843..1265)
                     /locus_tag="VN647_08155"
     CDS             complement(843..1265)
                     /locus_tag="VN647_08155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWG97040.1"
                     /translation="MAGKILLEVVTPEKQLLSLQVDEVIAPGSEGEFGVLPGHCHFLS
                     TLRIGELRYRVDGQTHSMAVLWGFAEVTPTKVTVMAEVAEKAEDIDVERATAKVTEAE
                     RRLQAGGLPSEVKEAQISLEKARLRKKIAERSQKTGHA"
     gene            complement(1396..>2597)
                     /gene="atpD"
                     /locus_tag="VN647_08160"
     CDS             complement(1396..>2597)
                     /gene="atpD"
                     /locus_tag="VN647_08160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWG97041.1"
                     /translation="APISVPVGRETLGRLINVLGEPVDEKGPLKTKKTYPIHRPAPKL
                     EDQETKTEVLETGIKVVDLLEPYSKGGKVGLFGGAGVGKTVIIMELINNIALHHGGFS
                     VFAGVGERTREGNDLWQEMQESKVIDPDDFTKSKAALVYGQMNEPPGARLRVGLTGLT
                     VAEYFRDEENQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPNLSTEMGALQERI
                     TSTKRGSITSVQAIYVPADDLTDPAPATAFAHLDATTVLSRSLAELGIYPAVDPLDST
                     SRILDPQIIGEEHYKIARGVQSVLQRYKDLQDIIAILGMDELSEDDKMVVARARKIQR
                     FLSQPFHVAEAFTGAPGKYVKLKDTVRSFREILDGKYDHLPEQAFYMVGPIEEAVAKA
                     EKMGVKV"
BASE COUNT          552 a          740 c          725 g          580 t
ORIGIN      
        1 gggagctgct ctgttgggat tggcaatagg aggcgcgatt gtcctgtacc cggctcgcac
       61 cttgcctagt accacatcga tctgtctgtc gatggacaag gtagccgact acatccatgc
      121 cgtcattgag gctgaccgag attcgtatac cagacatgtc gtagaacgca tgcaaacgaa
      181 agggatcgtc gtcgcctcgg aaaattggga agacatgcac accttgcccc tgccggccca
      241 atttctattg gaatccggtc ggcacctcga taagaagggc ataggcatac agtatcggct
      301 gatcagcttg tggccgatca acaagcgaaa tgtcgcgaca actcccctcc aaagtattgc
      361 gctcggtact attgtgactc aacccaacca tcccttcacc ggggtcacga aggtaggcga
      421 cacccgttat tatgaagcgg tgtatcccga tctggccgtc actcaggcct gtatcggctg
      481 tcataatgcc catcccaaca gcccgaagcg agacttcaag gttaatgatg tcatgggtgc
      541 gatcgtgatc agtattcagt tgggacccgg tttccctgga tagaggatca gaccgccatc
      601 cggtcatggg cgtgatcgtg atcagtattc agttgggaca gtagccgtcc tgcagcgtcg
      661 agcagcaacg gccactgaag cctatgggcc cttgagaaat cgcacaacac agtaaccagc
      721 attcttgacc ggcggagtga tctcagctct gtcggtcagg cgcaatggga tgccggaaga
      781 gactgtgcgt taggagattg cataactgag gatgcaagtc atgcagtaag agaattgatg
      841 gtttaggcgt ggccggtttt ttgagaacgt tcggcaattt tcttgcgaag acgagccttt
      901 tcgagactga tctgggcttc tttgacttcg gacggcaagc cgccggcttg aaggcgtcgc
      961 tccgcctcag tgaccttggc agtagcccgt tccacatcga tgtcttcagc tttttcggca
     1021 acttccgcca tgacggtgac tttggtcggt gtgacttcgg caaagcccca cagtacggcc
     1081 atggaatggg tctgaccatc gacacgataa cgaagttcgc cgatgcgaag agtggagagg
     1141 aaatggcagt gcccaggcag cacgccgaac tctccctcag acccaggggc aatgacctca
     1201 tccacctgca ggctcagcag ctgcttctcc ggcgtcacga cttctaacag aatcttcccc
     1261 gccatgattt cttttcccta attaccttcc ccctagaggc gaggaagcga ggcgtcctcc
     1321 actgcgcgca tgcaacgagg gtcttccgag accgcgcgtt gcgcgagcaa ggaggatgct
     1381 cggttcctcg cctccctaaa ccttcactcc cattttctcg gctttcgcca ccgcttcttc
     1441 gatcgggccg accatataga acgcctgctc cggcaggtga tcgtacttgc cgtcgaggat
     1501 ctctctgaag ctgcggaccg tatctttgag tttgacgtac ttgccggggg cgccggtaaa
     1561 tgcttcggcg acgtggaagg gctgcgagag gaaccgctgg atcttccggg cgcgagccac
     1621 gaccattttg tcatcttccg acaactcgtc cataccaaga atcgcgataa tgtcttggag
     1681 gtccttgtac cgctgaagga cggactgcac gccgcgcgca atcttgtaat gctcctcacc
     1741 gatgatctgt ggatcgagaa tacgcgaagt cgagtccaga ggatcaaccg ccgggtaaat
     1801 gcccaactcg gcgagcgacc gagataacac ggtggttgca tccaagtgtg cgaacgccgt
     1861 cgccggagcc ggatcggtca agtcatcggc cggcacgtag atggcctgca cggacgtgat
     1921 cgaacctcgt ttggtcgatg taatgcgttc ctgcagggca cccatttcgg tcgagaggtt
     1981 cggctgatat cccaccgccg agggcatgcg gcccagcaat gcggacactt ccgaacccgc
     2041 ctgagtaaac cggaaaatat tgtccacgaa gagcaatacg tcttgattct cttcgtcgcg
     2101 gaaatattcc gcgaccgtga ggccggtcag tcccacgcga agtcgcgcgc cgggcggctc
     2161 attcatctga ccatagacca acgcagcctt ggacttggtg aaatcgtccg gatcaatgac
     2221 cttcgattcc tgcatctcct gccacaggtc gttcccttct cgggtgcgtt cgccgacgcc
     2281 ggcgaatacg gaaaatccac cgtggtgcaa cgcgatgtta ttgatgagct ccatgatgat
     2341 gacggtttta ccgacgccgg cgccgccgaa cagtccgacc ttgccgccct tactatatgg
     2401 ttcgagtaaa tcgacgactt tgataccggt ttccagcacc tcggtcttgg tttcttgatc
     2461 ttccagcttt ggagcagggc ggtggatcgg ataggtcttc ttggtcttga gaggcccctt
     2521 ttcgtcaacc ggctcaccga gtacattgat gaggcgacca agcgtttcac ggccgacggg
     2581 tacagagatc ggggctc
//
LOCUS       DATNDD010000339         4139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4160963, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000339 DATNDD010000000
VERSION     DATNDD010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4160963"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..964
                     /locus_tag="VN647_08165"
     CDS             428..964
                     /locus_tag="VN647_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97042.1"
                     /translation="MRRGEVRRLKKLPGLPKEYRKELLKELEKLRSEQILTVDHVLEA
                     TAAASALRKRDVLTLKGEDDLRCAIIKKFRTKVENNTVAPRLLTKLARGVHRNEVSLT
                     VAKRVSEKLIEQGSYSISDAFRDSVEQSDFQHNLEQLVARLLDKLKEHERRGYQMSSK
                     LLNSLKVLANRIGHLSEE"
     gene            1944..2606
                     /locus_tag="VN647_08170"
     CDS             1944..2606
                     /locus_tag="VN647_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWG97043.1"
                     /translation="MLTLIQFPWSPFCITIRHILTRHRIPFRLHNIAYHDRAPIIKAT
                     KGQGHTVPCLIDGRHAICDLTDFGQEVAHYVDRRYRLNLFPKNLDGLQLILSRYIEND
                     LESVGFKVNDTYVIPTRPLIERTMLIRHKERKFGRGCLREWTTHRRYLCTQFVELLKP
                     IDSMLADSPFLLTDRPLFVDYNLYGVLGNYLFNGKTKLPSLKHLRRWYQMMDTRNYPD
                     LS"
     gene            2677..3075
                     /locus_tag="VN647_08175"
     CDS             2677..3075
                     /locus_tag="VN647_08175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97044.1"
                     /translation="MPSDKPASQPSPADRREFYRITVTLPICLQLESDQAEGTLIQRS
                     VNISGGGIGVTLNQTFAVNEILSCALLLPGQVLFESFLEVLRVDPITYPSNTYRLHGR
                     FVRMANQDRELLIRHILKFQREHLHRHYSA"
     gene            3187..3558
                     /locus_tag="VN647_08180"
     CDS             3187..3558
                     /locus_tag="VN647_08180"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97045.1"
                     /translation="MEPTRPYFRHHYRVPLAVQYPVMFSDTETIAEGKVMNLTVFGCA
                     IECAGAAPQRTTLRVRLILPDQAQSLPVEEAEVRWVQGNRMGLQFHKVERAADFRLHG
                     FVWDRMIERLRTITQEGISIS"
     gene            complement(3534..>4139)
                     /locus_tag="VN647_08185"
     CDS             complement(3534..>4139)
                     /locus_tag="VN647_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWG97046.1"
                     /translation="ALAFWILGAGSSAGQTIMAALLSVGLLVCSIAGFVFIQQRGLFA
                     SILSLVKKLGVRIRSLEAQEAHLRSIDQTIRNFYSHHQKAFYTSMVVYFLGWMAESME
                     VFGIIYLLGGSVSVLSALSIGALAVFIKGGSFFIPGSLGAQDAGNLLLLQAFGYSDVT
                     GITFALLRRFRELVWIGIGLLCLATVGKGRIPRDQEIEIPS"
BASE COUNT         1094 a         1140 c          987 g          918 t
ORIGIN      
        1 ggctgcgcgc tcatcacgct tcgcaaggtt ttgaaatgac ttctagttga tcctagcctt
       61 attaagagaa atgaggacaa tcctcgcctc atattccgcc aaaaggaact tgacgaactt
      121 cagagcagta tcgagaagca aggaatactc gtccctctcg ctgtctttaa agaaggaaac
      181 ggatttcggt ttttagacgg cgaacgaaga tggcggtgtg cgcttaagat cggactatcc
      241 tctgtccccg taatagtcca accgaaacct gccaggttgc aaaacctaat gatgatgttc
      301 gccatacatc atgaacggag ggaatgggat catctgccaa cagcgctcaa acttgaggaa
      361 ctagaaaaga gttgaggcca aacaaggccg taaaccttct gaggtggaat tagctgggct
      421 tgcttcgatg cgtcgaggcg aagtacggcg actaaagaag ctcccgggtc tccctaaaga
      481 gtatcgcaag gaacttctca aagaactcga aaagctaaga tctgaacaaa ttcttactgt
      541 cgaccatgtc ttggaagcaa cagctgcagc cagtgcgctt cgcaaacgcg atgtcttaac
      601 actgaaaggg gaagacgatc tacggtgtgc cattatcaaa aagtttcgaa ccaaggtcga
      661 gaacaataca gttgctccga gacttttgac taagctggca agaggtgtac acagaaatga
      721 ggtctcactg actgtggcaa agcgagtttc cgaaaagctc attgagcagg gaagctactc
      781 catatcagat gcattcagag actctgtcga acaatccgac tttcagcaca atttggagca
      841 attggttgcg cgtcttctcg acaagctaaa agagcacgag cggcgtggat atcagatgtc
      901 tagcaaactc ctcaacagtc taaaggtttt agctaatcgg atagggcatc tcagcgagga
      961 gtaggcggcg tggacaggcg cacgtatcaa actctcgcgc ctgagttaga agaatacgct
     1021 gatcgagttg ctcaacactt tcaggaccat ggatacagga tacagtttga aaagaaggac
     1081 ttaggattcc cctatacccc gactctcgtt gcaatgagag agcgcacaac tctcatacta
     1141 gaggttgatt cttcaataaa acaccttcgt ttagaagcat gggctcgcta tggaaaatcc
     1201 tgcgggcatg acctacgtgt cattgtttgc cttccggatc acatccaagt tgcttctaaa
     1261 tcaagaacaa cttcgctcta aaagctttgg tctatcagcc atttcgagtt ctggaatcgc
     1321 acagatcatt gctccaatcg acctgaacct gaacattgcc ttacccgaga ggcgatcgct
     1381 tcccacaaga ctaaaagctc tcctttgtgc tgcatacgat cacttcgacc agggatcatg
     1441 gcgcgacggt ttcgaggaag ctagcagggt gttagaaatc caaagtcgtt gctaccttaa
     1501 gcgatggatt aagaccagcc gtatacatac aattgattgc aaatggtaag ccgaggaagc
     1561 tcacgggtcc tcaaattgaa aaacttccaa tgggatcact ggcaaaacat ttcggcgaga
     1621 tagctgccaa gaacagtacc gactctcaaa tcgagaaagc tttagcaaca gtaaacaaag
     1681 atcgcgtttc tgtcgtgcac caccgacata aactgagaac cgaaactagg ctgcgtcaaa
     1741 acgtaggaag gcacatgtgg acaatcgtcg aaacactgaa acttcttgcg tgaccaccat
     1801 aaactactca gaaaccgccc atgctctggc cgattcgaac cgccagcgaa ttcagatgat
     1861 tccaccgacc ttgctgacac tagcggctat caatgcaacc atcgacgctt gcggtttcgc
     1921 ttcgcttcct tcatataagc tctatgctca ccctcatcca gtttccctgg tctcccttct
     1981 gcatcacgat caggcacatc ctgacacgcc atcgtattcc gtttcggctt cacaatatcg
     2041 cctatcatga tcgagctccg atcatcaaag cgacaaaagg tcagggtcac acggtgccct
     2101 gcctgatcga cgggaggcac gccatctgtg atttaacgga cttcggacag gaggttgcgc
     2161 attatgtgga tcggcgttac cggcttaacc tctttcccaa aaacctcgat gggctacaac
     2221 taattctttc gcgctatatc gagaatgatt tggagtcggt gggattcaag gtgaacgata
     2281 cgtatgtgat tccaacccgt ccactcatcg aacggacgat gctcattcgc cacaaggagc
     2341 ggaaattcgg aagaggatgc ctccgagaat ggaccaccca ccgacggtat ctgtgcactc
     2401 agtttgtgga actcttgaag ccgatcgaca gcatgctggc ggactcaccg tttcttctga
     2461 ccgatcgccc gttgttcgtg gattacaatc tgtacggcgt actcggcaac tacctgttca
     2521 acggaaagac taagttgccc agtctgaagc acctccgacg ctggtatcag atgatggata
     2581 cgaggaacta tcctgatttg tcttaagaca tttacgtacg cttttcaatt acggctcatt
     2641 ctattcatcc tgctttttgc tatactcttc cgcaccatgc catcggacaa gccagcttcg
     2701 cagccgtcgc ctgcagatcg gcgcgagttt tatcgcatca ccgtcacctt gcccatctgc
     2761 cttcagcttg agagcgacca ggcggaggga acgctcatcc agcggtctgt caacatcagc
     2821 ggcgggggta tcggcgttac actcaaccag accttcgcgg tgaacgaaat tctctcctgc
     2881 gccctgctcc ttccaggcca ggtgctcttt gagtccttcc tcgaagtgtt gcgtgttgat
     2941 cccatcacct acccgtccaa cacctaccgc ctccatggcc gcttcgtcag gatggccaac
     3001 caggatcgag aattgctgat cagacatatc ttaaaattcc agcgcgaaca cctccatagg
     3061 cactactccg cctaaatggg cgcgcctctc accctgaatg ggatcaaaaa aacctcaagg
     3121 attccgcttc gtccccgcaa aagggggatt cattctgcca cgcaggaccg actacactcg
     3181 gccaccgtgg aaccgacacg tccctatttt cgacaccact atcgagtccc cctcgccgtg
     3241 caataccccg taatgttttc cgatacggaa acgatcgcag aagggaaggt gatgaacctt
     3301 acggtgttcg gctgcgcaat cgagtgcgcc ggtgccgcgc cgcagcggac caccctccgc
     3361 gtgcggctaa tcctccccga tcaggcacag tccctcccgg tcgaagaggc cgaggttcga
     3421 tgggtccaag ggaatcgaat ggggcttcag tttcacaaag tagagcgggc ggcggatttt
     3481 cggcttcacg ggtttgtgtg ggaccggatg atcgaacggc tccgaacgat cactcaggag
     3541 ggaatttcta tctcttgatc ccgaggtatc ctgccttttc caaccgtcgc caaacacaac
     3601 agcccgatgc cgatccatac gagctcacgg aaccgtcgca agagcgcgaa agtgatcccc
     3661 gtcacgtcac tgtagccgaa cgcttggagc aacagaagat tgccggcatc ctgggcaccc
     3721 aggctgccgg ggatgaagaa agatccaccc ttgataaaca ccgcgagtgc ccctatagag
     3781 agggcggaca gcacactcac cgaaccaccg agcaaataga taatcccaaa gacttccatc
     3841 gattcggcca tccagcccaa aaaatacact accatcgacg tataaaaggc cttttgatgg
     3901 tggctgtaaa aattccgaat cgtctggtcg atcgagcgca ggtgcgcttc ctgcgcttcc
     3961 agagaccgga ttcgcacgcc caatttcttg accaatgaca gaatcgacgc aaagagccca
     4021 cgctgttgga tgaagacaaa tcctgctatt gaacacacca aaagtccgac acttaacaat
     4081 gccgccatga tagtctgccc cgccgaactg ccggcaccca gaatccaaaa ggcaagggc
//
LOCUS       DATNDD010000340         4414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_155387, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000340 DATNDD010000000
VERSION     DATNDD010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155387"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VN647_08190"
     CDS             <1..701
                     /locus_tag="VN647_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWG97047.1"
                     /translation="PTMPTFKYTLSEDEIWAIVGHVRTLHGMKMEFNVSARKTSLEEG
                     LKAAQAKFDQAKQAYEAAEKKASDEAERKSEQLKKDVDVDESAYAAEQAAMVQAKKEM
                     DTAQIALNNFSTRPGKGLSIPRPDLTIKPAEAAKLVDRGKRLYENKYGCNGCHNLDGE
                     GGKIGPALDRAGFRLNATWIYRWLKNPQAMDAHTRMPALGLTDGDAKAVTMYISTLRA
                     PKAEPVAEKSVEKP"
     gene            complement(890..1774)
                     /gene="ubiA"
                     /locus_tag="VN647_08195"
     CDS             complement(890..1774)
                     /gene="ubiA"
                     /locus_tag="VN647_08195"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWG97048.1"
                     /translation="MSGPAVSSSASWGIPWPALARLIRLPNQTGTYLLLLPTMWGLVL
                     AARGIPPLHLVAIFIGGSFLMRSAGVIMNDLADQSFDRQVTRTKTRPLASGELSQRHA
                     AILLAVLLVVAASLLFFLQPIVTWLAPVAVFLAALYPYSKRWLHIPQAMLGIAFGWGT
                     VMAWAAVRGQLDAPVWCLFGATASWAVAYDTIYAIQDQEDDRRIGVKSAALYFGSAVH
                     QGVGWAFGAMLVFLTAAGWFAQLGWPYYAALLGVAVFFLLQIGRLRRPITPTHAFEMF
                     RKHVWVGIAILAGLLAGC"
     gene            1835..3898
                     /locus_tag="VN647_08200"
     CDS             1835..3898
                     /locus_tag="VN647_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97049.1"
                     /translation="MRIRYNHKVNKLQPMLSIHFPPCRHVISFYVVLALALSGVGCSL
                     LKRGKEPVSSQPIPLTPVIWFSPSVTTAEVPYLDGCGETRSVTLTDALVASVPKKLTD
                     VFTAVTAQSQADETIASDGVIEVGVGLRRIELAAPKQAQGIYPATATVGIEMVFLARD
                     GALLFSKKLEGTGRGTVTVGEQSCQIAGVEAIVQDAMDSAIIGLAQQIAQSVQIREYA
                     AQRGAEVPTAARPHFSAAASTAETATSTPPVMETASQQLSVASTAPAVPAQLSFRAII
                     RDENRDQFLQPDESITIELEVKNEGMGEAKDVAVTAEGKAELTAFFPAEVQIGNLKPG
                     EIKRTSISQRVTAPQEHLHGELTLNLRTTSPVVSVHPPKVFSFGVKPKVIDPALVPDV
                     DNMPNSLAAFTQPKAVIISIGVGTFRDGQVPAIKYARHDAEVMAEYLRIIGGVPGERI
                     RILLDRQALEPDLEDTFERWLRKKVDRETVVYVFFAGRALVEGGTGAISLVPYDGMPS
                     GAKQLYSLVRLQEVLYRLPIRRAILMFDVSMDPSPGAGLADIPSPAWESSVSEARKDV
                     EMWMVSNRGLQEAHAYDEGKHGLFTYHLLRGLQGAADVDRDGTVTAGELCTYARGEAA
                     RVAREQFGNKQDALCVPGIGREGMVRIYPVARGNNPKPVPMQKQPNLPADSSGPAPNP
                     MQIGP"
     gene            3995..4070
                     /locus_tag="VN647_08205"
     tRNA            3995..4070
                     /locus_tag="VN647_08205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4028..4030,aa:Thr,seq:cgt)
BASE COUNT         1035 a         1214 c         1337 g          828 t
ORIGIN      
        1 ttcccactat gccgacattc aaatatacgc tgtccgaaga tgaaatttgg gcgatcgtcg
       61 ggcacgtgcg cacgttgcac gggatgaaga tggaatttaa tgtctcggcc cgcaagacct
      121 cgcttgaaga agggttgaaa gccgctcagg ccaagtttga ccaggccaaa caggcctatg
      181 aagcggcaga aaagaaagcc agcgatgaag cggagcggaa gagtgagcag ttgaagaaag
      241 atgtggatgt ggatgaatcc gcctatgcgg ccgagcaagc ggccatggta caagccaaga
      301 aagagatgga tacggcacag attgcactga acaacttctc taccagaccg ggcaaagggc
      361 tcagtatccc aaggccggat ctgacgatca agcctgcgga agccgccaag ttggtcgatc
      421 gcgggaaaag actctacgaa aataagtatg gctgcaacgg ttgccacaat cttgacggtg
      481 agggtggcaa gatcggacct gccctggatc gagcgggatt tcggctgaac gcgacctgga
      541 tctaccgctg gctgaagaat ccgcaggcta tggatgcaca cacgcgcatg ccggcactgg
      601 ggctgactga tggggacgca aaggccgtga ccatgtatat ctccacgtta cgtgctccca
      661 aagcggaacc tgttgcagag aagtcggtcg aaaagcctta gaccagcgcg gaaaaactca
      721 gttgatgcca aaaatatgac cggaggtttc ggtgcggtgc taactccgga ataccttcag
      781 gatgctcaaa aaggctgtcc agcaaggccg cagcaagcga agaggaagag gcgtacgctt
      841 cagtacgttg agcctctgag cgatgcgaga acgccgctgg cggacttttt cagcatcctg
      901 cgaggagccc ggcgagtatc gcgatcccga cccatacgtg tttccggaac atctcgaatg
      961 catgagtggg tgtgatcggc cttcgtagcc ggccgatctg gagcagaaaa aataccgcca
     1021 cgcccaagag agccgcgtaa tatggccatc ccagctgagc gaaccaccct gctgcggtca
     1081 agaaaaccag catggcgccg aacgcccaac ccactccttg gtgaacggca gatccgaagt
     1141 acaaggccgc cgacttgacc ccaatgcgtc gatcatcctc ctgatcctgg atcgcataga
     1201 tcgtatcata ggctacggcc caggaggcgg ttgctccaaa gagacaccac accggtgcat
     1261 ccaattgtcc ccgcaccgcg gcccaagcca tgaccgttcc ccacccgaag gcgatgccca
     1321 gcatggcttg tgggatatgg agccatcgtt tggagtaggg gtagagggct gcgagaaaga
     1381 ccgcaaccgg ggcgagccag gtcacgatcg gttgaaggaa aaacaggagg cttgccgcca
     1441 cgacaaggag cacggctagt agtatagccg catggcgttg cgataactca cctgaggcca
     1501 agggacgagt tttggtacga gtaacctgcc ggtcgaacga ttgatccgcc aaatcgttca
     1561 tgattactcc cgcgctccgc attaagaacg atccgccgat gaaaatggct actagatgga
     1621 gcgggggaat cccccgggcg gcgagcacca gcccccacat cgtgggaagg agcaagagat
     1681 aggtaccggt ctgattcggc agacggatca atcgggcaag cgcaggccac ggaatccccc
     1741 aggacgctga agatgacact gcgggaccgg acatgacgcg accttagccc acaggaaaca
     1801 ggctgtcaat gcgtcttcgc cggttgtgac gccgatgcga attcggtata accacaaggt
     1861 gaacaagctg caacccatgc tgagcatcca tttcccgcca tgccgtcacg tcatctcatt
     1921 ctatgtggtg ctggcactag ccttgtcggg tgtcggctgt tccttactca agcgggggaa
     1981 ggagccggtc agttcccaac cgattccatt gacgccggtg atctggttct cacccagcgt
     2041 gaccacagcc gaggtgccat acctcgatgg ttgcggcgaa acgagatcgg ttacgctgac
     2101 tgacgcgctt gtcgcctccg ttcccaagaa gttgacggac gtctttaccg cggtcacggc
     2161 tcagagtcag gctgatgaaa ccattgcgtc cgacggcgtg atcgaggtag gggtaggact
     2221 gaggcggatc gagcttgccg cccccaagca agcgcaagga atctatccgg cgacggcgac
     2281 ggtaggaatt gagatggtct tcttggccag ggacggagcg ttactcttca gtaagaagct
     2341 cgaaggtacc gggcgtggta cggtgacagt cggggaacag tcgtgtcaga tagcgggcgt
     2401 ggaggcgatt gttcaggacg ccatggactc ggctatcatc ggcttggctc aacagatcgc
     2461 gcaatcggtg cagatccgag aatatgccgc acaaagaggc gccgaggttc cgacggcggc
     2521 acgtccacat ttctctgcgg cggcgtcgac ggcggagacc gccacgtcca cccccccggt
     2581 gatggagacg gcgtcgcaac aactgtcggt cgcttcgacc gcgccggctg tgccggcgca
     2641 actgagtttt cgcgccatca tccgggatga aaatcgggac caattccttc agccggatga
     2701 atccatcacg atcgaactcg aagtgaagaa cgagggaatg ggagaagcaa aagacgtggc
     2761 ggtcacagcg gaaggaaaag ccgagctcac ggctttcttt cctgcggaag ttcaaatcgg
     2821 caacctcaag ccgggagaga tcaaacgcac ctcgatcagc caacgggtga cggcgcccca
     2881 agaacatctt cacggagaat tgacgttgaa cttgcggacg accagtccgg tggtgtccgt
     2941 tcacccgccc aaggtattta gttttggggt caaacctaaa gtcatcgacc cggcgttggt
     3001 tccggatgtc gacaacatgc cgaattcttt ggcggcgttt actcagccga aagccgtcat
     3061 catttcgatc ggcgtcggga cgtttcgtga cggacaggtg ccggcgatca agtatgcccg
     3121 ccatgatgcg gaagtcatgg ccgaatattt gcgtatcatc ggcggtgtcc caggagagcg
     3181 catacgaata ttgctcgacc gccaggctct cgaaccggat ttggaagaca cgtttgagcg
     3241 atggcttcgg aagaaggtgg accgggaaac cgttgtctat gtgttttttg ccggacgagc
     3301 actggtagag ggcgggaccg gggcgatttc actcgtgccg tatgatggga tgccctccgg
     3361 ggcgaagcag ctctattccc tcgtccggct acaggaagtg ctctatcgac ttccgatccg
     3421 acgggctatt ctgatgttcg acgtctcaat ggatccctct ccgggcgccg ggcttgccga
     3481 catcccctcg ccggcctggg aatcttctgt cagcgaggca aggaaagatg tagagatgtg
     3541 gatggtgagc aaccgaggcc tgcaggaagc ccatgcgtac gacgaaggca agcacggctt
     3601 gtttacgtac catcttctca gagggttgca aggcgcggcc gatgtggatc gcgacgggac
     3661 ggtcaccgcc ggcgaactct gcacctatgc tcgaggtgag gcggcgcgtg tggcgcgtga
     3721 acaatttggg aacaagcaag atgcgctttg tgttccagga atcggacggg aaggcatggt
     3781 gcgaatatat ccggtggcga gaggcaataa cccgaagccg gttccgatgc aaaaacaacc
     3841 gaaccttccc gccgactcat caggaccggc tcccaatccg atgcagattg ggccctagtt
     3901 aagtgaggcg gcctgcttca ttgacagtac tcggcgatga ccgttatcat gccgagcaag
     3961 gcctcatccc gtctttcatc cgtctcacca aggtgccggc gtagctcagt tggtagagca
     4021 gcggtttcgt aaaccgcagg tcgcccgttc aatccgggtc gccggctcca tacaatgctt
     4081 gccccgaccg cgagctcttt atcacagtgt cgtccatcga agcgaacgga gaatcaccag
     4141 ctattcagac gccaggtgct atctaaaagc ctttcctata gactaccgct aagatcctaa
     4201 caccggtttt ttcatggaag ggcatgtgcg ggaggcatgg accatgcgat agacgatcaa
     4261 agcgcgatac caccatgtgt cttggagccg ttggagccac tcgccctcgc cgatgaggcc
     4321 gaggttcaag tcacggtcga aacaacggga gccgttagcg cggaagagat tctgcagcgg
     4381 gccgcccgcg tttatcaggg attgaccgcc gagc
//
LOCUS       DATNDD010000341         9902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_690999, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000341 DATNDD010000000
VERSION     DATNDD010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690999"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="argH"
                     /locus_tag="VN647_08210"
     CDS             <1..804
                     /gene="argH"
                     /locus_tag="VN647_08210"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWG97050.1"
                     /translation="NVMPLGSGALAGTNYPVNRRYTAELLGFPAVTLNSLDAVSDRDF
                     MIESASALSIVMMHLSRLSEELIVWASQEFQFVDLPDAFCTGSSMMPQKKNPDVPELV
                     RGKTGRVYGHLINLLTLLKALPLSYNRDLQEDKPALFDALDTVTASLQVMTELMRRLK
                     INGEALKHALQGSGLLATELADYLVMRGVPFREAHGITGRIVRAALDQGRDVTDFSLE
                     ELRVFCERIDKSVFSRLTTAAAIDHKGQIGGTARRRVEQRIKELERLLS"
     gene            1067..2338
                     /gene="lysA"
                     /locus_tag="VN647_08215"
     CDS             1067..2338
                     /gene="lysA"
                     /locus_tag="VN647_08215"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWG97051.1"
                     /translation="MHSFEYHHGELYCEQVPVSRVAKELGTPCYIYSHATLVRHFHAY
                     DSAFKNTPHVIAFAMKANSNLAILRMMAKEGSGADIVSGGELFRALKAGVPASKIVFA
                     GVGKSSDEIRDALKADILMFNVESSAEIHAINEVASSVGKKARIALRINPDVDPKTHP
                     YISTGMKKSKFGIASDRALEEYKVASSLSHIDVVGVHAHIGSQLTDVTPFVDSLKKIV
                     ALIDTLKSQEINIRYLNIGGGLGITYSEEKPPLPQDLADAISPLLKNLGLTLVMEPGR
                     VIVGNAGILVTRTLYEKAGETKHFVIVDAAMNDLIRPSLYGAYHEIRPVQEEAIRRAK
                     QTVDIVGPVCESGDFLAKDRSLPGVKPGELLAVMSAGAYGFVMASNYNSRPRVPEILV
                     KGGEYHVIRDRETYDDLIRGESIPSFLSETE"
     gene            2347..3219
                     /gene="dapA"
                     /locus_tag="VN647_08220"
     CDS             2347..3219
                     /gene="dapA"
                     /locus_tag="VN647_08220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWG97052.1"
                     /translation="MFAGSLVAIVTPFRQGKVDERALAELIEWQIANGTNGIVPCGTT
                     GESATLSHSEHNRVIELTVEVVRRRVPVIAGTGSNSTEEAIALTKHAKQAGADGALLI
                     TPYYNKPTQEGLYQHYRAVAENVDLPLVLYNIPSRTGVNMLPSTIARLSVINTIVGVK
                     EGSGSVQQASDIVQQCGDRLTVLAGDDSLTLPMMAVGGKGVITVTANIIPLEMAGLVK
                     AFADGRIDEARRIHFKLSPLFAALFFETNPIPIKEALGLMRKIDPELRLPLCPMAQDT
                     REKLIRVLKDAALI"
     gene            3234..4052
                     /gene="dapB"
                     /locus_tag="VN647_08225"
     CDS             3234..4052
                     /gene="dapB"
                     /locus_tag="VN647_08225"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWG97053.1"
                     /translation="MHDNKRIKVIVAGAAGRMGSRLISLIRDSTALTMAGALEGKGHP
                     SLGEDTGETAGSGRTGLLITDDLSILMERGEVVIDFSSPVATLEHMRTVAHHRRAMVI
                     GTTGFSAVQLDELKALAQQIPCVFSPNMSVGINLICKVIGEMARTLGDDYDIEVIEAH
                     HRLKKDAPSGTALKIAEVLARSVNRDLSQVGVYTRKGLIGERIKGEIGIQTIRAGDIV
                     GDHTILFGGMGERIELTHRASSRDTFVQGALRAARWVVRQPAGLYDMMDVLSLR"
     gene            complement(4430..4582)
                     /locus_tag="VN647_08230"
     CDS             complement(4430..4582)
                     /locus_tag="VN647_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97054.1"
                     /translation="MKTCDKCHGAMLPERAVDLDAGLAITVFACLNCGRRKAADQEPR
                     PITARH"
     gene            complement(4811..4951)
                     /locus_tag="VN647_08235"
     CDS             complement(4811..4951)
                     /locus_tag="VN647_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97055.1"
                     /translation="MAHWILDHLVLFFILSASGRFFPITSNWSFESTKHLSEQEVENR
                     EK"
     gene            complement(5131..5442)
                     /locus_tag="VN647_08240"
     CDS             complement(5131..5442)
                     /locus_tag="VN647_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97056.1"
                     /translation="MQRFVWLLFFPLIISSCSGKNPYLEASLKPAELQGKDKGWFEKN
                     WGAPDSKASRFFGGETWTYYRIAGGTSGKPLFNFSPNQCQITLKFDKDEKLSDYTYSG
                     C"
     gene            5614..6261
                     /gene="fsa"
                     /locus_tag="VN647_08245"
     CDS             5614..6261
                     /gene="fsa"
                     /locus_tag="VN647_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HWG97057.1"
                     /translation="MKIYLDTANIKEIHEAASLGLLDGVTTNPSLVVKEGRSFREMLQ
                     EVCKIVDGPVSAEVVSVEADAMVKEGQELAKIHKNIVVKCPLIPEGLKATKRLAAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSNGMELIRQILTIYKNYAYKTLV
                     LVASVRHPQHVVEAALAGGHICTMPYSVFQALFKHPLTDAGLKKFLDDWKAKGQQ"
     gene            complement(6398..7102)
                     /locus_tag="VN647_08250"
     CDS             complement(6398..7102)
                     /locus_tag="VN647_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWG97058.1"
                     /translation="MVHCGHMRALTLLNKTIIDCTACHRLVVYRQAIARQKRKQYRDW
                     IYWGRPVPGFGDRHARLYILGLAPAAHGGNRTGRIFTGDRSGDWLYDALYRYGFANQA
                     ASQHRDDGLSLTDCYIGATVRCAPPGNKPAPDEFERCGRFLQEEVCLLKNHRVVIALG
                     KIAFDHFLKTCRTQGHAMPAPGPKFGHGVVYRLPWGVTLLGSYHPSQQNTFTGKLTRS
                     MFHAVFRMARKEIDRA"
     gene            7204..8253
                     /locus_tag="VN647_08255"
     CDS             7204..8253
                     /locus_tag="VN647_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWG97059.1"
                     /translation="MIIRRMRSLCSMVGIGFFILSMCPSALATSETTDNGGPKSDVEK
                     DLLVPDLLDSQPEQEDRLIILPEIKREGERFFLSSFKLPDKITFAGVPVPLDNWQVRE
                     RVEYEFYQFLEDQGESIVLAKRTGRCFPPAEKQLADAGLPDDLKYMLLVESKCISAAY
                     SRAKASGPWQFIPSTGRRYRLKSDAVRDERRNLEMSTEAAVKYLKYLKDFQDNDWFLA
                     MASYNAGEERIRKLLKEQKIGDYWKMHGPRETMRYVPRIIAAKEIYSQPEKYLGLTKK
                     DLYMPLETETITVNVKDSQRALTSIAEEFGTYLLELKMLNPEFKKDMLPHGSYQIRVP
                     RQTCPSRCFKQEKMP"
     gene            8258..9595
                     /locus_tag="VN647_08260"
     CDS             8258..9595
                     /locus_tag="VN647_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWG97060.1"
                     /translation="MRLRLSPSPARPLLQKLITAGLSTADPYQALLKHVELDRHSLIV
                     GRRAYDLSHIDRVIAVGAGKASARMAQALEAALGTRLGNGLVIVKTGHTLTTKRIAVV
                     EASHPIPDRAGLQATRRLLRLTQHLTPRDLLIVLLSGGASSLLPAPVAGVTLADKQRT
                     TRLLLRSGATINEINVVRKHLSLVKGGGLAVSTRARIVTLLLSDVIGDDLGSIGSGPT
                     AEDPSTFADAVGVLHRYRCWHAVPSAVRHYLHHGQKGDAPETLKSGSRRLRSVQHHII
                     GNNRMMLEAVAHAAQQAGLHTKFVSHPIMGEARVAAKHLTDLAKAITEGPGSLKRPCC
                     VIAGGETTVTVTGRGKGGRAQEFAASAAFEIAGLSNTWVVALGTDGTDGPTDAAGAIV
                     SGYTVARAKKLGIDLRSSLNRHNTYPALKALKCHIHTGPTGTNVNDLYLVLLL"
     gene            9601..>9902
                     /locus_tag="VN647_08265"
     CDS             9601..>9902
                     /locus_tag="VN647_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWG97061.1"
                     /translation="MPTYGPSFILPLSQCSNGNLTGGKAVGLAQLLAAGFSVPQGFCI
                     TTETYIQCLLASGFREDEEWHKACALSGNERASALADCRTRIKQVETSQLAVQWRMA"
BASE COUNT         2355 a         2611 c         2723 g         2213 t
ORIGIN      
        1 aatgtcatgc cgctcggatc cggcgccttg gccggtacga actatccggt gaaccgacga
       61 tatacggctg aactgctggg ctttcccgct gtcaccctga acagcttgga tgccgtttcc
      121 gaccgtgatt tcatgattga atcggcatcg gccctctcga tcgtgatgat gcatttgtca
      181 cggctgagcg aggaattgat cgtatgggcg tcgcaggaat ttcagttcgt cgatctgcct
      241 gacgcattct gcacgggaag cagcatgatg ccgcagaaga agaatcccga cgtccccgaa
      301 ctggtccgag gaaaaaccgg tcgcgtctat ggccacctca tcaatctgct gaccctgctc
      361 aaggccctgc cgttaagtta taatcgggac ttgcaggaag acaaaccggc gctctttgac
      421 gcccttgata cggtaacggc ttctctccaa gtgatgacgg aattgatgcg ccgactgaaa
      481 atcaacggag aggccctgaa gcacgccttg caaggaagcg gacttctcgc cacggaactg
      541 gccgactact tggtcatgag aggcgtgccg tttcgcgaag ctcacgggat caccggacga
      601 atcgtccggg ctgcgcttga tcaaggacgt gacgtcacgg atttttccct tgaagaactt
      661 cgtgtatttt gtgagcggat cgacaagagt gtgttttctc ggttgactac cgcagcggca
      721 atcgatcaca aagggcaaat cggcgggact gcacggagac gagttgagca acgaatcaag
      781 gagcttgaac ggctactgtc atgagagctg ttgtcattat tgtatcggca gggttggtga
      841 tttcttgtgg agtgataggg tcaccggtcc ctccggaata cgccggagtt gcgcccacga
      901 ttgagaaaca gaaaaggcaa catgccctcg agacagagcg gcaagcggat gggtcggatc
      961 ctgtcctggg agcccaggac atcgacctgc ccccatcgca gccggtggga atacggtaaa
     1021 agatctaggg tgtgatgcaa tcggtttaac acaaggaacc ctcgcgatgc atagtttcga
     1081 gtatcaccac ggtgaattgt actgcgaaca ggtaccggtc agccgcgtgg caaaggagct
     1141 cggcactccc tgttatatct atagccatgc gacgctcgtc cggcatttcc acgcctacga
     1201 cagtgcattc aaaaataccc cgcatgtcat cgcattcgcg atgaaagcca attccaatct
     1261 ggccatcctt cgcatgatgg cgaaggaagg cagcggcgcg gatatcgtgt cgggcggcga
     1321 gctgttccga gccctcaaag ccggcgtacc ggcttccaaa atcgtttttg ccggcgtcgg
     1381 taaatcctcg gatgaaattc gcgacgcgct gaaggccgac atcctcatgt tcaatgtcga
     1441 gtcgtcggct gagatccatg cgatcaacga ggtggcgtct tcggtcggga aaaaggcacg
     1501 gatcgcgtta cggattaatc cggatgtaga ccccaaaaca catccttaca tttcgacggg
     1561 gatgaagaaa agcaagttcg gcatcgcctc ggatcgcgcg cttgaagaat acaaagtggc
     1621 gtcctcgctg agccatatcg atgtggtagg cgtccatgcc catatcggct cgcagttgac
     1681 ggatgtgacg ccgttcgtcg attctctcaa gaaaatcgtg gcgctcatcg atacgctgaa
     1741 gagccaggag atcaacatcc gctacctgaa tatcggaggc gggctcggca tcacctattc
     1801 cgaggagaaa ccgcctttac cccaggactt ggctgatgcg atttcgccgc tcctgaaaaa
     1861 tttgggactg acgctggtca tggagccagg ccgcgtcatc gtcggcaacg ccggcatcct
     1921 ggtaaccagg acgctgtacg agaaggccgg ggagacgaag cactttgtca ttgtcgatgc
     1981 cgccatgaac gatttgattc gaccgagctt gtacggtgcc taccacgaaa ttcgccccgt
     2041 acaggaggaa gccatccgtc gggccaaaca gacggtggat atcgtcgggc ctgtgtgtga
     2101 atcaggagat tttttggcca aggatcgatc gttgccgggt gtcaaacccg gtgagttgtt
     2161 ggcggtcatg agcgcgggag catatggctt tgtgatggca tcgaattaca actctcggcc
     2221 tcgtgtgccg gaaatcctcg tcaagggagg agagtatcac gtcattcgag atcgagagac
     2281 atacgacgac ctcatacgag gcgagagcat tccatcgttc ctcagcgaaa cggagtgagg
     2341 gtgagaatgt ttgccggatc gcttgtcgct attgtcacac cgttccgaca gggcaaagtg
     2401 gacgagcgtg ctttggccga gttgatcgag tggcagattg ccaatggcac caacggcatt
     2461 gtcccctgcg gaaccacagg tgagtcggcc acgctttccc atagcgagca taatcgggtg
     2521 attgagttga cggtcgaggt ggtccgccgg cgggtaccgg ttattgcagg aaccggttcg
     2581 aacagcacgg aagaagccat cgcgctcacg aaacatgcga agcaggctgg ggccgacggc
     2641 gcgttgctca ttacccctta ctacaacaaa ccgacccagg aaggactcta tcaacactat
     2701 agagcggttg cggagaacgt tgacctgccc ttagtcctat acaacatccc gagtcgcacc
     2761 ggagtcaaca tgttaccgtc aacgatcgcc cgactctcag tcattaacac gatcgtcggg
     2821 gtcaaggaag gaagtggctc cgtccaacag gcatcagata tcgtgcagca gtgcggtgac
     2881 cgcttaaccg tgttggctgg cgacgattcc ctcacgttac cgatgatggc ggtcggtggg
     2941 aagggtgtaa ttactgtaac ggccaatatc ataccgttgg aaatggccgg ccttgtgaaa
     3001 gcctttgccg atggcagaat cgacgaagcg cgacggattc attttaagct ctctcccctc
     3061 tttgcggcgt tgttctttga gaccaatccc attccgatca aagaagcgtt gggactcatg
     3121 aggaagattg acccggaatt gcgcttgccg ctctgtccaa tggctcaaga cacgcgcgag
     3181 aaattgatcc gcgtcctgaa agatgcggcg ttgatctaac tagcaatatc tagatgcacg
     3241 acaacaagag gatcaaggtt attgtagcgg gtgcggcagg ccgaatgggc agtcgactga
     3301 tatcactgat cagagattct acggctttga ccatggcagg agccttagaa ggaaaagggc
     3361 atccatcgtt gggagaggat acgggcgaga ctgcaggatc aggccgtacc ggccttctca
     3421 tcacggatga tctctctatt ctgatggagc gaggagaagt ggtgatcgat ttttcctcgc
     3481 ccgtggcgac acttgagcat atgcggactg ttgctcacca tcgacgggcc atggtaatcg
     3541 ggactaccgg cttttctgct gttcaactcg atgagttgaa agcactggcc cagcagattc
     3601 cctgcgtctt ttctccgaac atgagtgtcg gaatcaacct gatctgcaaa gtcatcggcg
     3661 agatggccag aacacttggg gacgattatg acatcgaggt cattgaagct caccacaggc
     3721 tgaagaagga cgcgccaagc ggcacggccc tcaagattgc cgaagtcctc gcccgttcgg
     3781 ttaatcgaga cttgagccaa gtcggagtct atacccgcaa ggggttgatc ggtgagcgaa
     3841 taaaaggaga gatcggcata caaaccattc gcgccggcga tatcgtcggc gatcatacga
     3901 ttctgttcgg cggtatggga gaacgaattg agctcaccca ccgagctagc agccgcgata
     3961 cgttcgtcca gggagccctg cgagccgcga gatgggttgt ccgccagccc gccggcttat
     4021 acgacatgat ggatgtctta agtcttcgtt gattcctcct tcatcgcttc acatcaccaa
     4081 gcagtcgatt gtgttttcag tgaaccctga ccaagtactt gcagtgcgtt catccaccgg
     4141 ctcctgtgca aagtacagct gactgaaaaa gacgactcgg caatttggtc gaattcaaga
     4201 ctggtggtta tgtagacagt tgcccgtgaa gcattcatgc tttcgggtga tttctgttgg
     4261 gtgcgtcgta atccgcatga tggccaaatg ttcattcata gagtattggc gtctccgaaa
     4321 agacacaggc tccgttaccc acattggcgt gaaagatgct ttacaagcac ctcatagccc
     4381 cccactcatc agaagatggc gacttggggt tcgacggtag gatttagaat taatgtcgag
     4441 ccgtgatcgg ccgaggttcc tgatccgctg ctttccgacg accacaattc aaacaggcaa
     4501 acacagtgat cgccagtccg gcatccaaat ccaccgctcg ttctggtagc atcgccccat
     4561 ggcatttatc gcaggttttc atggggagca ctttagcacc ataggatagg gtaagccaat
     4621 cctcctgaag tactggatgg cactgacaag agtcccaatg ggacctatga acataggtcg
     4681 tttgcgcatg tttccatcag tagtatctca cgataaattg caggtagtta tcttgacagg
     4741 atcccgccgc tttcatagga ttgtggtcct cttgcgatag accactatgt atagattaat
     4801 cctaatcgtt ttacttctca cggttttcta cttcttgctc cgacaaatgt ttcgtggatt
     4861 caaaactcca attcgaggtg atcgggaaaa atcggccgct cgcgctcagg atgaagaaca
     4921 agaccagatg atcgaggatc cagtgtgcca catattcgtc ccgaagcgaa ttgccgtgat
     4981 caaaatgatc ggaggacggg agtactgttt ctgcagccag gagtgtgcgg tgagatttcg
     5041 gagcgagcac cccatctagc ggggtattca gaaactatat gaaatcgtcg ccattgaaga
     5101 aaaatccagc aagtttttgc accaagttga ttagcagcct gaataggtat agtcggaaag
     5161 tttttcgtct ttgtcaaact tgagggtgat ctgacattga ttgggagaga aattgaagag
     5221 tggcttccct gacgttcctc cggcaatacg atagtaggtc caggtctctc caccaaaaaa
     5281 acgagatgct ttgctgtccg gagcacccca gtttttttcg aaccaccctt tatcctttcc
     5341 ctgaagttcg gccggtttca gtgaagcttc taggtagggg ttcttccctg aacaagatga
     5401 aattataagg gggaagaaca gcaaccagac aaatcgttgc atcgtgacat ctcctttaag
     5461 ctcaagagtt gctattaaac gaaaaattct atcctcagct caaaagcctg tcaaactttc
     5521 gtgtcgactt cttgcagtga ggttgagttc cccataaaat gattgataat aacggtgtcg
     5581 cctcacgatc tcttcgctat gaaaggaaca gctatgaaaa tctatctcga tacagccaac
     5641 atcaaggaaa tccacgaagc ggcaagcctt ggcttgctcg acggcgtgac tacgaatcct
     5701 tccttagttg tcaaagaagg cagaagtttc agggaaatgt tgcaagaggt gtgtaagatc
     5761 gtggacgggc cagtcagcgc cgaggtcgtg agcgtggaag cggacgccat ggtgaaggaa
     5821 ggtcaagagc tcgccaagat ccacaagaac atcgtggtca aatgtccact gattccggaa
     5881 ggactgaaag ctacgaagcg gttggctgct gagggcatca gagtgaacgt gactctctgt
     5941 ttctctccga cacaggcgct gctggcggct aaagccggtg cctggtgtgt gtctccgttt
     6001 ataggacggc tcgacgacat cagctcaaat ggcatggagc tcatccgaca gattctgacg
     6061 atctataaga actatgccta taagaccttg gtgttggtgg caagcgtccg tcatccgcag
     6121 cacgttgtcg aggcggcatt agccggcggt cacatctgca cgatgcccta cagtgtgttt
     6181 caagcgctct tcaaacatcc gctgaccgat gccggactga agaagttctt ggacgactgg
     6241 aaagcgaaag gacaacagta gaagttgaca agtgggggtg ggctccgtgt tcgacggatg
     6301 ttcaccccac tctaaccact gctcgcctct caaacggaag ctgcgcggaa acaggacccg
     6361 ccaacaccaa cacgtacatt gttgacattg tcttaagcta cgcgcggtca atttcttttc
     6421 tagccatccg aaacaccgcg tggaacatcg agcgagtcaa tttcccggta aacgtgtttt
     6481 gctggctggg gtgataggaa cccagcagtg tcacacccca tggtaaacga tagacaacac
     6541 catgcccaaa ttttgggccc ggcgccggca tagcatgccc ctgagtccga caggtcttga
     6601 ggaaatgatc gaaggcgatc tttcccaggg caatcaccac acggtgattc tttaggagac
     6661 acacctcctc ctgcaagaat cggccgcagc gttcaaactc atccggtgca ggtttattcc
     6721 ccggcggtgc gcaccgcacg gttgctccga tgtaacaatc cgtcaacgat aaaccatcgt
     6781 ctctatgttg cgaggcagcc tggttggcaa atccataccg atacagggcg tcatacagcc
     6841 aatccccgct ccgatcaccc gtgaaaatac gccccgtccg atttccacca tgtgcagcgg
     6901 gagccagtcc aaggatatag agccgagcgt ggcgatctcc aaagccggga accggccgcc
     6961 cccagtagat ccaatcacga tattgtttcc gtttctgccg tgcaatcgcc tgtcgatata
     7021 cgaccaaccg atgacaggcc gtgcagtcga tgatagtctt gttcagaagt gtcagagctc
     7081 gcatatggcc gcagtgtacc acgaacggga atcctattct gcttgctggt gaataggtca
     7141 gctgctagca tgacctcgca ttttgtgacg gcttgcatct ggacgcaatg gaggagtttg
     7201 agaatgatca tccgtcgtat gcggtccttg tgttctatgg ttggaatcgg tttctttatc
     7261 ctgtcaatgt gtccttcagc cctggccacg tcggaaacaa cggataatgg cggtccaaaa
     7321 tcggatgtgg agaaggattt gctcgtgccg gatctgctgg attcgcagcc tgaacaagaa
     7381 gaccgtctga tcattttgcc ggaaatcaaa cgggaaggag aacggttctt tctgagctca
     7441 ttcaaactgc cggacaagat cacgtttgcg ggagtgccgg ttcctctgga taattggcaa
     7501 gtgagagaac gagtcgaata cgagttctat caatttttgg aagatcaggg tgaaagcatc
     7561 gtcctcgcca aacgtaccgg gcgttgtttc cctcctgccg aaaagcaatt ggccgacgcg
     7621 ggtttgccgg atgatctcaa gtatatgttg ctggtcgaga gcaagtgcat ttcagccgcc
     7681 tactcgagag cgaaggcatc cggtccctgg caattcatcc cctccaccgg gcgccggtac
     7741 cgactgaaga gcgacgccgt ccgagatgaa cgtcgaaatc tcgaaatgtc gaccgaagcg
     7801 gcagtgaagt acctcaagta cctcaaagat tttcaggaca acgattggtt cttggccatg
     7861 gcctcctaca atgccggtga ggaacgaatc cgcaagctgc tcaaggagca aaaaatcggc
     7921 gactattgga agatgcatgg cccacgcgag accatgcggt atgttcctcg tatcattgcg
     7981 gcgaaggaaa tctactccca gccggagaag tatcttggac tgaccaagaa ggatttgtat
     8041 atgccgcttg aaaccgaaac catcacggtg aatgtcaagg actctcagcg cgcgctgact
     8101 tcgatcgccg aagaattcgg cacctatctg ctcgagctca agatgctgaa tcctgaattc
     8161 aagaaagata tgctccctca tggcagctat caaatccgcg tgccccgtca aacctgtccc
     8221 agtcgctgtt ttaagcaaga aaagatgccg tagtgctatg cgacttcgcc tttccccttc
     8281 tcctgcacgg ccactccttc aaaaactgat caccgcaggg ctgagtacgg ccgatcccta
     8341 ccaagccctg ctcaagcacg tcgagcttga tcgccactcc ctgattgtgg gccgtcgcgc
     8401 ctatgatctc tcgcatatcg accgtgttat cgcggtcggc gccggcaagg catcggcaag
     8461 gatggcacag gcattagagg cagccttggg gacaaggttg ggaaatgggc tcgtcatcgt
     8521 caagacaggt catacactga ctaccaaacg gatcgccgtg gtcgaagcca gccatccgat
     8581 tcccgatcgt gcagggcttc aggcaacccg acggcttctg cgcctgacac aacatctgac
     8641 acctcgagac ctgctgatcg ttctcttgtc cggaggtgcg tcaagcctgc taccggcccc
     8701 agtcgccggt gtgacgctgg ccgataagca acgcacgacg cgcctgctgc tccgcagcgg
     8761 tgcaacaatc aatgaaatca acgtcgtgcg gaaacatttg tcattggtca aaggtggggg
     8821 gttggccgta tccacccggg cgaggattgt taccctcctc ctttctgatg ttatcggtga
     8881 tgatctcggt tctatcggct cggggccgac agcagaagat ccctcgacat ttgcggatgc
     8941 agtgggggtg ctgcaccggt atagatgctg gcatgcggta ccttctgccg tacgccacta
     9001 cttgcaccat ggtcaaaaag gagatgcgcc agagaccttg aagtccggct cacggaggtt
     9061 gcgctccgtg cagcatcaca tcatcggcaa caaccgaatg atgctggaag cggtcgcgca
     9121 tgctgcacaa caggcaggcc tccacaccaa gttcgtctct caccctatca tgggagaagc
     9181 acgtgtggca gcgaagcact tgacagacct tgccaaggca attacagagg gacctggtag
     9241 cttgaagcga ccttgttgcg tgatagcggg aggtgagacc acggtgacgg tcacaggccg
     9301 tggaaagggt ggacgtgccc aggaattcgc ggcttctgcg gcatttgaga tcgccggcct
     9361 ctccaacaca tgggtcgtgg cactgggcac tgacgggacc gacggaccca ctgatgcagc
     9421 cggggcgatt gtcagtggat acaccgtcgc gcgagcgaag aaactcggca ttgatcttcg
     9481 gtcatcatta aatcggcaca atacctaccc tgccttgaaa gctcttaaat gtcatatcca
     9541 taccggtcct actggaacga atgtcaacga cctttacctc gtcctgctgc tctagctact
     9601 atgccgactt atggcccgtc ctttatcctt ccgctgtctc agtgcagcaa tggcaatctc
     9661 acagggggaa aggccgtcgg gctcgctcag ctcctcgccg ccggcttctc tgtccctcaa
     9721 gggttctgca tcacgacgga gacgtacata cagtgtctgc ttgcgtcagg gtttagggag
     9781 gatgaagagt ggcataaggc ctgcgcatta tcaggaaacg aacgagcgtc tgcgttggcc
     9841 gactgccgaa ctcgcatcaa acaggtagag acttcgcaac ttgccgttca atggcggatg
     9901 gc
//
LOCUS       DATNDD010000342         3506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1848193, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000342 DATNDD010000000
VERSION     DATNDD010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848193"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VN647_08270"
     CDS             <1..856
                     /locus_tag="VN647_08270"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWG97062.1"
                     /translation="GYLANIGVIPNLIEQGGLILADRLCHASLIDGCRLSRADFRIFH
                     HRDCAHLESLLRRRKPNRPTLIITEGLFSMDGDLAPLSDLTSLAEQYEAMLYVDDAHG
                     TGIMGPTGRGTIEHFGLEQRIPFHMGTMSKALGSHGAYIVGPNDLMQYLINVTRPFIF
                     TTALPPAIAAAAAAAVAVIRGEPERRMRLWSNRQQLFNGMQKLGFRMTQTVSPILPIL
                     VGDAAVASALAERLFTHGIYASAIRPPTVPNGTSRIRFTVTSEHTTEQIDEALHALDL
                     AGRETGLL"
     gene            974..2032
                     /locus_tag="VN647_08275"
     CDS             974..2032
                     /locus_tag="VN647_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWG97063.1"
                     /translation="MDISKLLTFSVKEGASDCHISAGEPPMIRIHGDLKKLDHPPLTP
                     DETHALIYDMMNDAQRKTFEEKRECDFSFELGDIARFRVNVFVQQRGLGAVFRNIPTT
                     ILPLEKLGMPPILRQLCDKEKGLILVTGPTGSGKSTTLAAMVDYLNNTFEGHIITIED
                     PIEFVHKSKKCLVNQRELGVHTLSFANALKSALREDPDIVLVGEMRDLETIQLALTAA
                     ETGHLVFGTLHTSSAPKTIDRIIDAFPPAQQAQIRTQLSEALEAVITQTLLKKKTGGR
                     VAALEVMVATTAVRNLIREAKLHQIPGIMQASQKDGMQTMDMALVDLATRGIVHKAEA
                     QSRSMNPNLFGAAMTGAA"
     gene            2059..3234
                     /locus_tag="VN647_08280"
     CDS             2059..3234
                     /locus_tag="VN647_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWG97064.1"
                     /translation="MDVRSLLKVMVDREASDLYLTVDASPIYRIHGATQPTDAPPFTN
                     EQLETLALALMRGQQRSEFEEKMEMNLALYYKELGRFRVNIFRQRGNVGLVFRHIKAD
                     IQTIEQLQLPPIIKDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNTVHQGHIITVED
                     PIEFVHQHKKSIITQREVGFDTLTFQNALKNTLRQAPDVILIGEVRDTETMEAAITFA
                     ETGHLCIGTLHSNNANQAIERIMNFFPVERHAQIYLQLSLNLRAIISQRLIPSIDGRR
                     VPALEIMLDTPRIKDLIKKAEVDTLKEAMEQGVDEGCQTFDQVLFQLYKTNKISLEQA
                     LINADSANNLRLKIKLEGLKGDEAVNALLDKQMGVGSTDAFKIQGGSSGNVTPLRKR"
     gene            complement(3249..>3506)
                     /locus_tag="VN647_08285"
     CDS             complement(3249..>3506)
                     /locus_tag="VN647_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HWG97065.1"
                     /translation="ANGQVGPPLVLGTTGVQRLKDPGYRGYAKTVHEYIVESVLEPQR
                     FVVRGYPGDTMPTWYGSKLSALALEKIAAYLERQTEERSVP"
BASE COUNT          830 a         1026 c          916 g          734 t
ORIGIN      
        1 cggttattta gcaaatatcg gcgtcattcc gaacctcatc gagcaaggag gcctgatcct
       61 agcggatcga ttgtgtcatg ccagtctcat cgatggatgt cgattgagcc gggccgattt
      121 tcggatattc caccatcgag actgtgcaca tctggaatca ttgcttcgac gacggaagcc
      181 gaatcgtcct acgctcatca tcacggaagg gttattcagc atggacggcg atctcgctcc
      241 tttgtcggac ctgacatcac tggccgagca atatgaggcg atgttgtatg tcgatgatgc
      301 ccacgggacc ggcatcatgg gaccgaccgg tcgcggaacg atcgaacact tcggcctgga
      361 gcagcggatc ccctttcaca tggggacgat gagtaaagcc ctggggagcc atggcgccta
      421 catcgttggg cccaacgact tgatgcagta tttgatcaac gtgacccgtc cgttcatctt
      481 cacaaccgcc cttccacccg cgatcgcggc ggcagccgcg gctgcggtcg cggtcattcg
      541 gggtgaaccg gaacgtcgga tgaggctctg gtcgaatcga caacagctgt tcaatggaat
      601 gcagaaactc ggctttcgga tgactcagac cgtcagtccg attctgccga tcctagtggg
      661 cgatgcagcc gttgcatctg cattggctga acgactgttc actcacggca tctatgcttc
      721 cgcaattcgc ccgccgaccg ttccgaacgg aaccagccgt atacgcttca ctgtgacatc
      781 ggaacacacg actgagcaga tcgacgaagc gcttcatgcc cttgacctcg ctggtcgtga
      841 gaccggcctc ctctaatcgc ttcctcatcg gacgcgtctt ccggctattt tcttgctttc
      901 gggctttgct atggcatgat gcacaggagc ttttcacctc acgagcaacc cataacactg
      961 aggatcacgg gcgatggata tttccaagct gctgaccttc tcggtcaagg aaggcgcctc
     1021 cgactgtcat attagcgccg gcgaacctcc catgattcgt attcacggcg atctgaaaaa
     1081 actcgaccat cctcctctca ctcccgacga aacacatgcc ctcatttacg acatgatgaa
     1141 tgacgcccaa cgaaagactt ttgaagagaa gcgagaatgt gacttctcgt tcgaactcgg
     1201 agacatcgcc cgtttccgcg tcaacgtgtt cgtgcaacag cgcggcttag gcgccgtctt
     1261 tcggaatatt ccgaccacca ttcttccgtt ggaaaaactc ggcatgccgc cgattcttcg
     1321 gcaactctgc gacaaggaaa agggattgat cttggtgacc ggaccgaccg gatccggcaa
     1381 atccaccacg cttgccgcga tggtggacta tctcaacaac acgtttgaag gtcacatcat
     1441 caccatcgag gaccccatcg agtttgtcca taaatccaag aaatgcctgg tcaatcaacg
     1501 cgagctcggt gttcacacat tgtccttcgc caacgccctg aagtcggcgc ttcgcgaaga
     1561 tccggacatc gtcctggtgg gcgaaatgcg ggacctggaa actattcagt tggcgttgac
     1621 cgcggcagaa accggacact tggtcttcgg gaccctccac acctcgagcg cgccaaaaac
     1681 gatcgatcgc atcatcgacg cattcccgcc ggcacagcag gcgcaaatca gaacccagct
     1741 atcggaggcg ttggaagccg tcatcaccca aacgctgttg aagaagaaaa ccggcggacg
     1801 tgtcgccgca ctcgaagtca tggtggcgac aacggcggtg cgcaatctca tccgtgaagc
     1861 caagctgcac caaattccgg ggatcatgca ggccagtcaa aaagacggca tgcaaacgat
     1921 ggacatggcg ttggtcgatc tcgcgacgcg tggaatcgtc cataaagccg aagcacagtc
     1981 ccgcagtatg aaccccaatc tcttcggtgc agcgatgacc ggcgcggcct agaacgcaca
     2041 taatatggag aacgtccgat ggatgttcga agtcttctca aggtcatggt ggaccgcgaa
     2101 gcgtcggacc tgtatttgac cgtcgacgcc tcgccgatct accgcatcca tggtgctacc
     2161 cagccgaccg acgctccccc cttcaccaac gagcagctcg aaacactggc gttggccttg
     2221 atgcgtggtc aacagcggag tgaattcgaa gaaaaaatgg agatgaatct ggcgctctac
     2281 tacaaggaat taggtcgctt ccgcgtcaac atcttcaggc aaaggggtaa tgtcggattg
     2341 gttttccgtc acatcaaagc ggacattcag accattgagc agctgcaact ccctccgatc
     2401 atcaaagata tcgcgatgac caaacgcggt ctcgtgctgg tggtgggagc gaccggctcc
     2461 gggaagtcga catcgttggc cgccatgatc gaccatcgca ataccgtcca ccagggccat
     2521 atcattacgg ttgaggaccc gatcgagttt gttcatcagc acaagaaatc gatcatcacg
     2581 cagcgcgaag tcggattcga cacgttgacc ttccaaaacg cgctgaagaa caccctccgg
     2641 caagctccgg atgtgatttt gatcggtgag gtgcgtgata cggaaaccat ggaagcggcg
     2701 attacctttg ccgagaccgg tcacctgtgc atcggaactc tgcactccaa caacgccaac
     2761 caagcgatcg aacgcatcat gaactttttc ccggtcgagc gccatgctca gatctacctc
     2821 caactatcct tgaatctgcg cgcgatcatc tcgcagcgat tgattccgtc gatcgacggc
     2881 agacgagtcc cggccttgga aatcatgctg gacacgccgc gcatcaagga cctgatcaaa
     2941 aaagcggagg ttgatacctt gaaagaagcg atggaacagg gggtggacga aggttgccaa
     3001 accttcgatc aggtgctgtt tcaattgtac aaaacgaaca agatctcact ggagcaagcc
     3061 cttatcaacg ccgatagcgc gaacaacctc cgtctaaaaa tcaaacttga aggactcaaa
     3121 ggcgatgagg ccgtgaacgc cttgctcgat aagcagatgg gggtcggcag cacggatgcc
     3181 tttaaaattc aaggaggctc ttccggaaac gtgactccgc ttcgaaaacg ttaagtgtcc
     3241 caatcttctc agggaactga cctctcttcg gtttggcgct ccagatacgc cgcgatcttt
     3301 tcaagggcca gcgcactcag tttggatcca taccaagtcg gcatcgtgtc acctggatac
     3361 ccacgcacca cgaatcgctg tggctccagc actgattcca cgatgtactc atgcaccgtc
     3421 ttggcgtatc cccggtaccc tgggtccttg agccgctgca cgcccgtcgt accgagtacc
     3481 aacggaggtc cgacttggcc gttggc
//
LOCUS       DATNDD010000343         3858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3445542, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000343 DATNDD010000000
VERSION     DATNDD010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3445542"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..876
                     /locus_tag="VN647_08290"
     CDS             214..876
                     /locus_tag="VN647_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWG97066.1"
                     /translation="MPLSWRPVFIALVGFLGCSSLILTDSLRAQSIEGLEAADNRQPA
                     PTNMSPEEARKHFLNCPGGPSTQFRLGGKVKNQKTFTLDTLRGQPAQTTVWAFYRSGS
                     SPTGFETGEWTGILLYDLLQQAQITLNAGVKNDLNRKVILVTGSDCYQQAFSMGELVP
                     AIGGQYQIIVAYAKDGVLFSSEGFARIINPGDKSGARNVSNIIRIQVIDPPLPQPSSN
                     SN"
     gene            932..1501
                     /locus_tag="VN647_08295"
     CDS             932..1501
                     /locus_tag="VN647_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97067.1"
                     /translation="MTPQRIGLWLYIGLVVLAYLSPVSLVSAQETAPSEPHTAMGHVT
                     GEHCRLSIGSTLENIQIETADIHLHEQLFESVLRATLVQRLDHPQTDRIRAYCLRHLL
                     VVIRQDLRTPRPTGWVQVNFVVSDVAALHQELEATARTALATLDVETRDKIVRFRLKP
                     GVMRNHRRVDRLEVYGPEGFLIGFDQPHQ"
     gene            1758..2150
                     /locus_tag="VN647_08300"
     CDS             1758..2150
                     /locus_tag="VN647_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWG97068.1"
                     /translation="MAKRMMGDVLTAREAARYVRLTLPTFYRYIWEGKIEAPRIGGRY
                     RFTKALLDRWLGKKNSGADDVSGRNKLVGRVTAIKRDAIMAQVDVDIGSHKITAVITR
                     DALDELRLRIRDTAIALIKATEVMIVKD"
     gene            2369..2530
                     /locus_tag="VN647_08305"
                     /pseudo
     CDS             2369..2530
                     /locus_tag="VN647_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            complement(2766..>3858)
                     /locus_tag="VN647_08310"
     CDS             complement(2766..>3858)
                     /locus_tag="VN647_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG97069.1"
                     /translation="LKPTLMDAQVLLIGLDATGGKLKEARKRLAPLLIQEPNNLVLLN
                     LLFQLQMYEKEWNGMQETLTRLRTAGADSVKADLAEGHVAVAQQQWGKAEAAYRQAIK
                     RRPQAAEPLLALVQLGVRRGQMHQVQATLETLLTEQPTHPFAAGFLGEVFLVKGDTAA
                     AMSRFEAATKLNPTWTLPWIHLARHFYAKQQIDAGDAWVRKALEASPTNESLRMILAT
                     SLGGRGRVDEAIAQYEFILKDHPKSLWAANDLASTLLDRKGDPDSLQRAMALTRDFER
                     HASNPYFLDTLGWTHLKLGHHQDALRLVKQAMIKAPGHPVLNFHLGVIYAQSGERKEA
                     RTHLVHALQNKQTFPWTEEAKATLARLDE"
BASE COUNT          892 a         1066 c         1039 g          861 t
ORIGIN      
        1 tatcgtcgtc gatcctcacg cgatcacatt gattcccagc cacggagaaa tcctctatga
       61 gcttgatgct tgggaatgca taccacattg acattctgca atcaggaggt ggctgcggca
      121 caaaaaaagg aaggccgacg atgcgggtat gttttccgtg cgagcgacgc agatcgacat
      181 attcatacaa ccataatgag gaaaggagcg tacatgcctt tatcatggcg acctgttttc
      241 attgcgcttg tcggcttctt gggatgttcg agcttgattc tgacggactc gctccgagca
      301 caaagcattg aaggtctaga ggcggctgac aataggcaac cggcgccgac caatatgtct
      361 ccggaggaag cgcgcaaaca tttcctcaat tgccccggtg gcccgtccac tcaatttcgt
      421 ttgggaggaa aggtcaaaaa tcagaagacc ttcactctcg atacgcttcg gggacaaccc
      481 gctcagacca cggtgtgggc cttttaccga tccggatcca gtccgaccgg attcgagacc
      541 ggcgaatgga ccggtatctt actctatgac ttgctgcagc aggctcagat cacgctgaat
      601 gccggggtga aaaacgacct gaaccgcaag gtaatcctcg tgaccggcag cgattgttat
      661 cagcaagcgt tttccatggg agagttggtt ccggcaatcg gagggcaata ccaaatcatt
      721 gtggcctatg ccaaggacgg tgtgctgttc tcgtccgaag ggtttgcccg catcatcaat
      781 cccggagaca agtcgggagc gcgcaatgtg tcaaacatca ttcgtatcca ggtgatcgac
      841 cctccattgc cacagccgtc atccaattcc aattaagagg acaaattcgc atgggcagca
      901 cagtggccta tccagtagga gcctcaaata cgtgacaccg caacggatcg ggctttggct
      961 atacatcggt ctcgtcgtcc tggcgtacct atcgccagtc tctctggtat cggcgcagga
     1021 gacggcgccg agcgaacccc ataccgcgat ggggcacgtc accggtgagc actgtcgatt
     1081 gtcgatcggc agtacactcg agaatatcca aattgaaacg gccgacattc atctgcacga
     1141 acaattgttc gaatccgtgc tccgtgccac cctcgtgcag cggctggacc atccgcaaac
     1201 ggaccgcatt cgggcctatt gccttcgcca cctcctggtc gtcatccgcc aggatctccg
     1261 cacgccgcgt cccaccggat gggtacaagt gaactttgtg gtgagcgatg tcgcggcgtt
     1321 gcaccaggaa ctggaagcaa cggctcgcac cgccttggcg acgcttgatg tggaaacacg
     1381 tgacaagatt gtgcggttcc gattgaagcc aggcgtgatg cggaaccatc ggagagtcga
     1441 tcgattggaa gtctatgggc cggagggatt cttgatagga ttcgaccaac cacaccaata
     1501 accgccggtt cccggcatac gacaatgtca tcgggagacg attggcatat atcagaaagg
     1561 ccttgcgacg ggtacgccct cccactgcaa ggccgttgca tccggaggag tgtgatgggg
     1621 tttcagcacc ttcggatgag aggagagccg caccactcag tcgtggctct cctctattag
     1681 tgataccggt cagacccatc gagaggcaat tcgtaataaa cgcttcagga ggttaattcg
     1741 atgataggag cccaacgatg gcgaaaagga tgatgggcga tgtgctcacc gccagggaag
     1801 cggcacgata tgttcggctg accttgccga ccttctaccg atatatttgg gagggtaaaa
     1861 tcgaggcacc aaggatcgga ggacgctatc ggtttacaaa agcattgctc gaccggtggc
     1921 tgggtaaaaa gaattccggc gccgatgatg tcagtggacg aaataagctg gtcggtcggg
     1981 tgacggcgat caaacgggac gcgatcatgg cgcaggtcga cgtcgacatt ggttcccaca
     2041 agattaccgc cgtaatcact cgtgatgcat tggacgaact cagactccgc atccgggata
     2101 ccgcaatcgc cttaatcaaa gcgactgaag tcatgattgt gaaagactag ctcgctcgat
     2161 tcgatcgacc tcttgccgaa tgagtctctg ccttctcctg aagccgattg atcccgtaca
     2221 gaccagcgag aaattcttac caaacgtaca tcattttctc atcgaacaat gctacgctgt
     2281 gattagcagt caggaggatt ctttacttac ataaggagga cagacaatga agctcagcgc
     2341 gcgcaatcaa ttttaaggta ccgtcgctcg catcacagaa ggccaggcga tggccgaggt
     2401 gacggtcaag gtcggcacac tcgagtttgt tgccgccatc accgaaggct cggtgaagag
     2461 catggggttg aaagtgaacg actctgtgac agtcgccatc aaagcgactg aagtgatggt
     2521 cggcaaataa cgccgttgct gatcgcatcg cggtagacct ctgcataaag ccctgcttgc
     2581 ccacagcgaa gaagcagggc tttgtgcccg cgcaattgac tgccagtcgt tgccggtagg
     2641 aacctcagtt atggcgaccg catgtcctgg tggttcagga aacctgcacc ccgccagcac
     2701 agtttgtcac tattccaatt tccagccttg agtcgatgaa tcgttctttt acctttccta
     2761 tgatgttatt catccagcct tgcaagcgtg gcctttgcct cttcagtcca gggaaaagtc
     2821 tgcttgtttt gcagcgcgtg caccaagtga gttcgtgctt ctttcctttc tcctgattgg
     2881 gcatagataa cgcctaaatg aaagttcagc accggatgac ccggggcttt gatcattgcc
     2941 tgcttcacca gccgaagcgc gtcctgatga tgaccgagtt tgagatgggt ccaaccgagc
     3001 gtatcgagaa aatacggatt ggaggcgtgc ctctcaaaat ctcgagtcaa ggccatggca
     3061 cgctgcaaac tgtccggatc tccctttcga tcaagaaggg tggaggcgag atcattggca
     3121 gcccacagcg acttggggtg atccttgagg atgaattcat actgtgcgat cgcctcatcg
     3181 acacgacctc gtccccccaa actagtcgcc aatatcattc gaagcgattc attggttgga
     3241 cttgcctcca atgccttccg aacccacgca tcacccgcat ctatctgttg tttcgcgtag
     3301 aaatgccgag cgagatggat ccacggtagc gtccaggtcg gattgagttt cgtcgcagcc
     3361 tcaaagcggg acatggctgc ggcagtatcc cccttcacca aaaacacctc gccgagaaat
     3421 cctgccgcaa agggatgcgt cggttgttcc gtaagcaggg tttcgagcgt cgcctgaacc
     3481 tggtgcatct gacctcgccg tacgccgagc tgcaccaatg ccaggagcgg ctccgcggcc
     3541 tggggccgcc gcttgatggc ttggcgatag gccgcttcag ccttgcccca ttgctgttgc
     3601 gcaacggcga catgtccttc ggccaaatcc gctttcacgc tgtccgcacc cgccgtgcgc
     3661 aatcgtgtca gcgtctcttg catcccgttc cattcttttt catacatttg aagctggaat
     3721 aatagattca acaacacaag attattcggc tcttggatga gcagtggggc gagtcgcttg
     3781 cgtgcctctt tcaacttgcc ccccgtcgca tccagaccga taagcaacac ctgcgcatcc
     3841 atcaacgtcg gtttcagc
//
LOCUS       DATNDD010000344         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3634051, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000344 DATNDD010000000
VERSION     DATNDD010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3634051"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..993
                     /locus_tag="VN647_08315"
     CDS             253..993
                     /locus_tag="VN647_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HWG97070.1"
                     /translation="MAIRWIGSPNRDKGREGFKPEAIVIHIMEGTLKGTDAWFSNEES
                     GVSAHYGIGKGGEIHQYVGESDRAWHAGRIVSPTWRLFKLDVNTNWYTIGIEHEGRAE
                     TVWSDALYDASARLIDEICRRWAIPCDRDHIIGHREIRSDKTCPGFEVDLDKLVDLAN
                     EFQRDPNTFNFIKKPGIVNTRIDLNIRELAPTTATAVVRTIKKGRKLTYKGWTSNGMT
                     VNGNAHWYRDSDDNYFWAGATERPIPGL"
     gene            941..1627
                     /locus_tag="VN647_08320"
     CDS             941..1627
                     /locus_tag="VN647_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97071.1"
                     /translation="MIITSGPEQPSGRFLDFETDILIRTGRHTLSTIALLLVGNLLLA
                     CSAPPISAYPSVQLTPDRQAILVIPSDRTTPSQLPLQSFPTESTLAPGLSYHPQRFEL
                     VRVSPNGRYAVFSAAGHHNLIGLLDLETMVVREVDLLTEGDVVAFHWAADGRTLAYDY
                     LPASGYHQVKGYDIESGEGLVVPRTERHSAAHITFDGWGPRPHEIILRITDIRSNERR
                     TETVTLIPRR"
     gene            1876..2268
                     /locus_tag="VN647_08325"
     CDS             1876..2268
                     /locus_tag="VN647_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97072.1"
                     /translation="MATQVCGLLASPSLSAVQSRMGTGEGCAWQWPNGQALSVYVHID
                     KQSETAAETKQMMDMYLQNPAFKRTAFPICKGGDMVIGDFRNGKGPGGTGYTQCSGFV
                     LTFSFQGADAQRNLPGIAKKLAGTTFAR"
     gene            2430..2606
                     /locus_tag="VN647_08330"
     CDS             2430..2606
                     /locus_tag="VN647_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97073.1"
                     /translation="MKNVEMSIEGSVLTIRVDLSKEFGPSSTGKTIIIASTEGNVTIP
                     DRQEKVGLNVYRKK"
BASE COUNT          681 a          770 c          783 g          635 t
ORIGIN      
        1 acgattgcag gaccaccacc aggaccgcaa caactctatt ccacagtgta tctgaggggg
       61 accgcacagt ttatcgtgtc ctaaccatga gctgctcgaa ctgatctcac tgtgaagagg
      121 acgaaaaagt gttacacaat cggccttggc gttatggcgc actcttgagt atgctttcct
      181 gtggagttgg gatggaaaac agctctcgcc tattcagaca ccgctgaatc agaggaacat
      241 ccggcggcaa gcatggctat tcggtggatc ggaagtccga acagagacaa ggggcgcgag
      301 ggattcaagc ccgaggcgat cgttattcac atcatggaag gcacgctcaa gggcaccgac
      361 gcctggtttt ccaacgagga gtccggggtt tcagcccact atgggatcgg gaaaggcgga
      421 gagatccatc agtatgtcgg cgagagcgat cgggcttggc acgccggccg gatcgtctct
      481 ccgacttggc gattgtttaa gctcgatgtc aatacaaatt ggtatacgat cggtattgaa
      541 catgaaggac gggccgagac ggtctggtcc gatgcgctgt acgacgccag cgctcggttg
      601 atcgatgaga tttgccgacg ctgggccatc ccctgcgacc gcgatcatat catcggccat
      661 cgagaaatcc gctcggataa gacgtgtccg ggattcgaag tcgatctcga taagctcgtc
      721 gacctggcca atgagttcca acgtgatcca aacacgttca atttcatcaa gaaaccgggc
      781 attgtgaaca cgcgcatcga tttgaacatt cgtgaactgg cccccaccac cgcaacagcg
      841 gtggtccgta cgatcaagaa ggggcggaaa ctcacctata aaggttggac gagcaacgga
      901 atgaccgtta acgggaatgc ccattggtat agggattccg atgataatta cttctgggcc
      961 ggagcaaccg agcggccgat tcctggactt tgagaccgat atactcatcc gcaccggtcg
     1021 ccacactctt tccacgattg cactcctttt ggttggaaac ctcctactgg cctgcagtgc
     1081 gccgcccata tccgcttatc cctccgtgca attgacgccc gatcgacaag ccattctggt
     1141 gattccctcc gatcgaacaa cgccttctca acttcctctt caaagttttc cgaccgaatc
     1201 aacacttgcg ccgggtctgt cttaccaccc gcagcgattc gagctggttc gcgtttcgcc
     1261 gaatggacga tacgcggtgt tttccgctgc aggacatcac aatctgatag gccttctgga
     1321 ccttgagacc atggtcgtgc gagaagttga tctcctgacg gaaggggacg tagtggcctt
     1381 tcattgggca gccgatggcc gaacacttgc ctatgactac ttgcctgcca gcggctatca
     1441 ccaggtgaag ggctacgata tcgaatccgg ggaaggtctg gtcgtgcccc gtacggagcg
     1501 tcattcagcc gcccatatca cgtttgacgg ctggggacca cggccgcatg agatcatctt
     1561 acgcataacc gacatacgca gcaacgaacg tcgaaccgaa accgtcacgc tgattccacg
     1621 ccgatagacg aaccgccatc agccctcgct gccgcaacct gatgagcatt gggacaggcc
     1681 attgaataga cgaagcgccg ggactacaat tcacctgttc gttccgaggc gtcaccgtga
     1741 acgtccgatg gtacggcgcg actcattcct ggagcgggtg tttttctttc aaggaaacgc
     1801 aacttgaaac ggagaggggg tttcatcgcc atttgcggat tactttgtgc atcacccgcc
     1861 cacgcaggga gccctatggc aacccaagtc tgtggtctgc ttgcatctcc ctcgttatcc
     1921 gcagtccagt cgaggatggg aacgggtgag ggttgcgcat ggcaatggcc caatggtcag
     1981 gcgttgtcgg tgtatgtgca catcgacaaa caaagcgaaa cagcggcgga aaccaaacag
     2041 atgatggaca tgtacttgca gaacccggcg ttcaagagaa cggcattccc gatttgtaag
     2101 ggaggtgaca tggtcatcgg tgattttcga aacggcaaag gtccaggcgg caccggatat
     2161 acacaatgtt ctggttttgt gctgaccttc agctttcaag gtgccgatgc tcaacgcaat
     2221 ttacccggca tcgcaaagaa actcgccggc acaaccttcg cccgctagcg tgctgatcct
     2281 gtgaggaaca ggtccattct caactcgaac caatgaagca cctcgtccgc gggctcgcct
     2341 ctggcgaact attgatctac cctgaacagg ttgcatagga tcgttgctca gggcagagcc
     2401 tccaatcatg ctactgaaag ggcgcaatca tgaaaaacgt tgagatgagc attgagggat
     2461 ctgtgttaac gatcagagtg gacctctcga aggagttcgg tccctcttca acgggcaaga
     2521 caattatcat tgcttcgacg gaagggaacg tcaccattcc ggatcggcaa gagaaggtcg
     2581 ggctcaatgt ttaccgaaaa aagtaggacc gatcgcagcc attcggccct gccttcttca
     2641 ccggcgaggc tgctttcttt tccgatcggc tcttgacaga tcagaaacag cgctctagac
     2701 gtatcaatga gtctgtgatc cccaggaaat tctcgaggct gtggatagcc atgcagcttg
     2761 tcgtcggacc aatgatttcg ggagggtgta ttatggatga cgatcttaca ctgtgtgaaa
     2821 ttgtctggaa gaaggatatg agccactatt agttgtggct cttctgata
//
LOCUS       DATNDD010000345         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_301758, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000345 DATNDD010000000
VERSION     DATNDD010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301758"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VN647_08335"
     CDS             <1..819
                     /locus_tag="VN647_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWG97074.1"
                     /translation="NDFLREALRYFPHLREDMFRIILVGAGKFILPELGEKLGAYAQR
                     KLREQKVEIHANCKVTAVTDRDVTLSDGTTVPTNTLVWTAGISPHSLLDTLPCPKTKG
                     RVLVNEYLEVPGWPGLWAFGDCALVPDLKTGGSHPPTAQHALREGRVAAQNILATVQG
                     DRMKPFLYSTLGLLAPIGKRTGVANILGINFSGFIAWWLWRTIYLLKLPRFEKKLHVA
                     LNWTLDVLFSKDLAHFRTKRSRIPHAAANRQKPTESFTPLFINRPPDFGLHKQE"
     gene            1075..1392
                     /locus_tag="VN647_08340"
     CDS             1075..1392
                     /locus_tag="VN647_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97075.1"
                     /translation="MNAKLLHIALVATTALSAAALGCSQTAAERRAASPTLGERIKQD
                     SVTGRVQDIGSKYVSIRESDGETTRVRVDDHTKMDRVAVGDQVKAYVSEDGYASTIQR
                     VSD"
     gene            complement(1421..2278)
                     /locus_tag="VN647_08345"
     CDS             complement(1421..2278)
                     /locus_tag="VN647_08345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWG97076.1"
                     /translation="MTDSANKEAGHGDEELNEASLVVNTSEAGPSRREILLHGSKVMA
                     GIGVAALLGNPGTFSRESYGMDRSAMSLLDSLTPLRTALLVIDMQRDFLLPEGYAAQA
                     GLDIAPLVATIRPIEKLLAVGRKAGLLIVHTREGHAPDLSDCPPYKLERSRRAGAEIG
                     SKGPLGRLLVRGEAGHDFVEALRPLEQEIVIDKPGYSAFTHTGLQQALAKRGIGTLIL
                     TGVTTEVCVSSTLRTAIDLGYRCITVSDACASSDLGLHKASLAMIGVEGGIFGEVATT
                     AEVIKRLAP"
     gene            complement(2538..>2846)
                     /locus_tag="VN647_08350"
     CDS             complement(2538..>2846)
                     /locus_tag="VN647_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97077.1"
                     /translation="SERLEDEVDHAGTKCLYSGIQIGICGHENHVGEEADRPLLGELI
                     ETVFSGHHVIEDHDIEMPSVEPVRRFLRIRRFLDRLTTWSESLDQEVPHPRLIINDQN
                     "
BASE COUNT          623 a          815 c          805 g          603 t
ORIGIN      
        1 aatgactttc tgcgggaggc gctgcggtat tttccccacc tgcgggagga catgtttcgg
       61 atcatcctgg tcggtgcagg gaaattcatc ttgccggaac tgggggagaa gctcggcgcc
      121 tacgcgcaac gaaaactcag agagcagaag gtggaaatcc atgcgaactg taaagtcacc
      181 gccgtgacgg accgcgacgt cacgctcagc gatgggacga cggtgccgac caatacgctg
      241 gtctggacgg ccggaatctc cccgcattcc ctcttggaca ccttgccctg tccgaaaacg
      301 aagggtcggg tgcttgtcaa cgagtatctg gaagtgcccg ggtggcctgg cctctgggcc
      361 ttcggcgact gtgcgctcgt gccggatctc aagactggtg gatcccaccc cccgaccgcg
      421 caacacgcgc tgcgtgaagg gagagtcgcg gcgcagaata tcctggcgac cgttcaaggc
      481 gaccgcatga aaccgttcct ctactcgacg cttgggcttc tggcccccat cggaaaacgg
      541 accggcgtcg cgaacattct cggcataaac ttctccggct tcatcgcctg gtggctatgg
      601 cggacgattt acttgctgaa actgcctcgc tttgaaaaga aactgcacgt ggccttgaat
      661 tggaccctgg atgtgctctt ctcgaaggat ctggcccact ttcgaacgaa gcgctcccgt
      721 atcccacatg cggcagcaaa ccggcagaaa cctaccgaat ccttcacgcc actcttcatc
      781 aaccgccccc cagacttcgg actacacaag caggagtaag gagacatgga gcgtctcgtg
      841 gaagatttgg agatcgccga acataagatt ccgatccata gcccggctgc cggccattgg
      901 cgcagatggt gtatgcgccg atcaaaaacc acagcaggtg atgatggatg aactgagcca
      961 agtcgagttc gttatagagg ccgatgcagc ggtctgcaac tagggtcact cccagcacgg
     1021 aatcatcgca cctctgggtt ataattaagt aagtcaattg aaggaggtgc tattatgaat
     1081 gccaaactgc tgcacatcgc cctcgtggct acaacggcat tgtctgccgc ggcgctggga
     1141 tgcagccaga cggcggccga acgacgtgca gcttcaccga ctttggggga acggatcaaa
     1201 caggattcgg tcaccggcag ggtccaggat atcggatcga agtatgtgtc catcagggaa
     1261 agcgacggtg aaacaacacg cgtccgcgtc gacgatcaca ccaagatgga ccgcgtggca
     1321 gtgggggatc aggtcaaagc ctatgtttcg gaagacggct atgcctcgac gattcagcga
     1381 gtctccgact aactatcggc cgaagaccaa ccgatcggta tcatggagcg agccgcttaa
     1441 tgacctcagc ggttgtggcg acttcgccga agatgccgcc ttccacgccg atcatggcca
     1501 acgaagcttt atgcaaaccg agatcgctcg aagcgcaggc atcactcacg gtaatacagc
     1561 ggtagccgag gtcgattgca gtacgcaacg tcgagctgac acacacctct gtggtgactc
     1621 ctgttaaaat gagtgttcca atgccgcgtt tcgccaacgc ttgttgcaat ccggtgtgag
     1681 tgaacgcact atacccgggc ttgtcgataa cgatctcctg ctccagggga cgtaaagcct
     1741 ccacgaaatc gtgccccgcc tcaccacgta ccaacagacg accgagagga cctttcgagc
     1801 cgatttccgc accggccctt cgactccgct ccagtttgta tggcggacaa tcggacaggt
     1861 ccggcgcatg gccttcgcgg gtatgcacga tcagtagtcc ggcttttcga cccactgcaa
     1921 gcagcttttc gatcggacgg atggtcgcga ccagcggcgc gatgtcgagt cctgcctgcg
     1981 ccgcataacc ctccggcagg agaaagtcac gctgcatgtc gatcaccagt agagccgtgc
     2041 gaaggggcgt cagtgaatcg aggagactca ttgctgatcg gtccattcca tatgattctc
     2101 ttgagaaggt tcccgggtta ccaagcaggg cagcaacccc tataccggcc attaccttgc
     2161 ttccgtgaag cagaatttca cgccgtgaag gaccggcttc gctcgtattg acgacaaggc
     2221 ttgcctcatt gagttcttcg tcgccatgac cggcttcctt atttgctgaa tcggtcatct
     2281 aagcctactt gcttgagtgc ttcacacgaa attcccgacc aatgtatacc actctctacg
     2341 tcttcctcaa agtaagacct tggctcttgg acaatacagt tacagaagtc cgcagcgttg
     2401 atgtgatagg ctggctgccg gttttggctg tctttttgtg gccggtgtcg ggagagaccc
     2461 gcatgtcgat ggattcaatt cacgaagtcg ttgagcctgt ggccgacccg gagttcgggc
     2521 catggttggc tcaagcgtca attctggtcg ttgatgatga gcctgggatg cggaacttcc
     2581 tggtcaagac tctcggacca cgttgtaagt cgatccagga agcgtcggat acgaaggaag
     2641 cgtcgcaccg gctcgacgct cggcatttcg atgtcgtgat cctcgataac gtgatgcccg
     2701 gaaaaaacgg tctcgattag ctcgccgagc agcgggcgat cggcttcttc gccgacgtga
     2761 ttctcatgac cgcatatgcc gatttggata ccgctataga ggcacttcgt gccggcgtgg
     2821 tcgacttcgt cctcaagccg ttccga
//
LOCUS       DATNDD010000346         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1065347, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000346 DATNDD010000000
VERSION     DATNDD010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065347"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..577)
                     /locus_tag="VN647_08355"
     CDS             complement(146..577)
                     /locus_tag="VN647_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HWG97078.1"
                     /translation="MIDRELITRKMSLILEDVAAMTKLSRLSRAQYLENSINEALAER
                     YLERAIGRMIDINFHLITESGHAPPKDYHESFIRLGTLGVMTSDLAKETAMAAGLRNR
                     IVHEYDDIDPERVHEALPVAVRRIPMYLDHIQRFIEKLPQE"
     gene            complement(574..1005)
                     /locus_tag="VN647_08360"
     CDS             complement(574..1005)
                     /locus_tag="VN647_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWG97079.1"
                     /translation="MSEEGNTEAVSGLRELAQRFRVCLILQFGSTVTGTTHDCSDLDL
                     AVQFETPHASFQTILEMQEALQARFPGCKVDLAILNRADPLFLKKIVESCRILFGMPQ
                     DLARLRLRAFKAYQDFRPYLELERRHVARRLSSLVAESSRP"
     gene            1161..2159
                     /locus_tag="VN647_08365"
     CDS             1161..2159
                     /locus_tag="VN647_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HWG97080.1"
                     /translation="MRAWLMDSYDGVEQLRLSEAPEPQPGPAEVLLKVEYAALNPADA
                     FLAQGLYPAKPTLPHILGRDGMGDVLQIGADVGNIRVGETVGILRCETGVETPGTLAE
                     KVVVPSESLVRIPSGWSLEEMAGAPLVFLTAWQALTQWNDPPVPPLARSVLLVTGASG
                     GVGVASVLLGKSLNLIVVALSRDARKRTKLKTLGADGVFDPEDKNLVKAISAAISPKK
                     VDLAVDCVGGKLFPQVIALLGYGGKISVVGRSGGTAPEFNTATLLFRRIRIGGVSVGD
                     YTAQAAQTAWKEIVDRLEMIGRRPLVDSIVSFEEVKKGFARLAQGPMGKVVVRVVG"
BASE COUNT          479 a          584 c          655 g          477 t
ORIGIN      
        1 aacaagacat caagggactg atccgcaatc aagataccgt ggtggcgacg gtcggagaaa
       61 aagccctcaa cgatcggtat ccttcactca tcagcggaat catccgacgc aatcttcaag
      121 actactcact tgaacgatca cctcatcatt cctgcggcaa cttctcaatg aagcgctgaa
      181 tgtggtccaa gtacatggga atccggcgga ccgcaacagg caaggcttca tgcactcgtt
      241 caggatcgat atcatcatat tcgtgcacaa tccggttgcg gagcccggcg gccatggccg
      301 tctccttggc aagatcggac gtcatcacgc cgagcgtgcc gaggcgtata aaggattcgt
      361 gatagtcctt gggcggcgcg tggccggact cggtgatcag atgaaaattg atgtcgatca
      421 tgcgaccgat ggcccgttcg agataccgct cggccagggc ttcattgatg gaattctcca
      481 gatactgcgc gcgtgacaat cgagatagct tggtcatcgc ggccacgtcc tcgagaatga
      541 ggctcatctt tctggtgata agctcgcggt caatcatgga cgggaagact ccgcgaccag
      601 cgatgacaat cgccgggcga catgccggcg ctccaattcc aagtaaggcc gaaagtcttg
      661 gtacgccttg aaggcgcgga ggcgcaggcg ggcgaggtct tggggcatcc cgaacaggat
      721 gcggcaggac tcaacgattt tcttgaggaa gagggggtct gcccggttga ggatagccag
      781 atctaccttg catccgggga accgcgcctg caatgcctct tgcatctcca gaatggtctg
      841 aaacgacgcg tgaggtgtct caaattgaac cgccaggtct agatcgctgc aatcatgggt
      901 tgtgcctgtg accgttgacc caaactggag gatcaagcaa accctgaaac gctgggcgag
      961 ttccctcaac cccgacactg cttctgtatt gccttcctcg ctcattcccc cgctcccagg
     1021 gtttcaccca atccgtagcc tagctgagaa tcgcttcatt gtctatgcgc ccccggttgt
     1081 tgcatgtgtt gtaatatcat tacggacgca tgggtaatga ctgtgaccgc cattgagcct
     1141 cgctaagagg aggtaagtct atgcgcgcct ggttgatgga ctcctatgat ggagtggaac
     1201 agttgcggct tagcgaggcg cccgaacctc agccgggccc ggcagaggtg ctgctcaaag
     1261 tggaatacgc cgcgctcaat ccagcggatg cgttcctggc gcaaggactc tatccggcca
     1321 aacccaccct gccgcacatc cttgggcgtg acggcatggg agacgtgcta cagataggag
     1381 ctgatgtagg gaacatccgt gtgggtgaaa cagtcgggat tcttcgctgc gagacgggag
     1441 tggaaacgcc tggaacactc gccgagaaag tcgtcgtccc ttcggaaagt ctggtccgaa
     1501 tcccctccgg ttggtctctc gaagaaatgg ccggtgcgcc gctggtcttc ttaactgcct
     1561 ggcaggcctt gactcagtgg aacgatccac cggtacctcc cttggcaaga tcagtcctcc
     1621 tcgtgaccgg tgcatcaggg ggagtcggcg ttgcgtcagt gttgctcgga aaatcgttga
     1681 atctgatcgt ggttgcttta tcgcgtgatg cacggaagag aaccaagctg aagacgctcg
     1741 gagctgatgg cgtgttcgat ccagaggaca aaaatctagt gaaggccata tccgctgcga
     1801 tcagtccgaa gaaggtagat ctggctgtgg actgcgtcgg tggcaaactc ttccctcaag
     1861 tcattgccct tctcggttat ggcggcaaga tcagcgtcgt cggcaggagc ggaggcacgg
     1921 ctccggagtt caatacggcg acgttgttat tccgccgcat tcgcatcggc ggtgtctcag
     1981 tcggggatta tacggcacag gcagctcaga ctgcgtggaa agagattgtc gatcggctgg
     2041 agatgatcgg acgccgtcca ttggtagaca gcatcgtttc attcgaagag gtcaagaaag
     2101 gattcgcacg attggcacag ggcccgatgg ggaaagtagt ggtgcgcgtg gtcggttaat
     2161 ggctgtgatt tgacatcgct ctattgttca tggct
//
LOCUS       DATNDD010000347         6998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3009418, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000347 DATNDD010000000
VERSION     DATNDD010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3009418"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VN647_08370"
     CDS             <1..562
                     /locus_tag="VN647_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HWG97081.1"
                     /translation="SLWQEENGRAYLYEDLRAMRIGDILTVKIAEDHKGSKSAETAVQ
                     RDSTINNSLAGSGAGYIGMPGLRFSDETKRQMGINASASYKFDGKGATNRTGTLTGTI
                     SVIVTEVLPNGDLRIEGRREVTVNSEKQLMSIGGIVRRVDVDTKNTVLSSAVADARIE
                     YSGLGVLDDVQRPGWFIRILDWVYPF"
     gene            864..2009
                     /locus_tag="VN647_08375"
     CDS             864..2009
                     /locus_tag="VN647_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HWG97082.1"
                     /translation="MITITRLATIFSALILTGCLLAPPSADAVRIKDIGVIEGVRENQ
                     LIGYGLVVGLDSTGDRVIGGQFTIQAMTSMLNKMGVNLLVNPIQLLTRNIASVMVTAK
                     LPPFSKPGMTLDAVVSSMANAKSLQGGTLLFTPLKAANQQVFAVAQGPVSIGGFLGGT
                     GGPGGATVVKNHQAAGLVPGGAIIEKEVSVDIDSWETVSVLLRQPDFTTAIRTAEAID
                     SAFGKGSASAVNAGSVKATIPQAFQGRIVEYIASIEGLDVTVDSIAKVVVNERTGTVV
                     LGEHVRISTCAISHGNLTISVKNTLNVSQPPAPLIGSSAGQTTVTPDVQTEIKEQESR
                     LIVVDETVTLGEVVRALNAVGVTPRDLVAILSALKSAGALQASLEVI"
     gene            2093..2416
                     /locus_tag="VN647_08380"
     CDS             2093..2416
                     /locus_tag="VN647_08380"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HWG97083.1"
                     /translation="MAPGSGHPDPQVNPSKSQNAAEARQQMVQAGQQFEAYFISYLLK
                     VMRETVPEGAITNKQGAYFYSFYDEEIGKRAAESGGIGIARMVQEYAQQYFASSPVEH
                     SSSSG"
     gene            2504..2809
                     /gene="flgM"
                     /locus_tag="VN647_08385"
     CDS             2504..2809
                     /gene="flgM"
                     /locus_tag="VN647_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWG97084.1"
                     /translation="MQVSGHGKVDHLAKLLLGVQETESSGGRQAAARPQVGKDEVQIS
                     AQAKELQRIRELANQPDSERTEHVERIRRALDGGTYDVSGRAVGDALMKQVLTDAIL"
     gene            2934..3449
                     /locus_tag="VN647_08390"
     CDS             2934..3449
                     /locus_tag="VN647_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HWG97085.1"
                     /translation="MPMSRFLPGIIQLCRLLHQEEATCHQLLETVHAERAAIRTLAIT
                     EFHSINCRRLSILESLQSLANERQHLVQEVGAHLGLQPVTTSIQELIDNVQDSASEDL
                     RTTYRSYMTVAKTVRDEIKQNVVLIEGIRGVVDQALSTGAPAVPGGDFYTADGQSFVA
                     GRVNMLIHQQG"
     gene            3452..5479
                     /gene="flgK"
                     /locus_tag="VN647_08395"
     CDS             3452..5479
                     /gene="flgK"
                     /locus_tag="VN647_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HWG97086.1"
                     /translation="MIGLTSLFDIARSALTTSQQALVVTGQNVANVNTPGYSRQEAVL
                     TERPPLNGQPGMVGAGVQVTSIRRYTDQFVNRQLTTVQQNLGRLSVSQEELSRLQNLF
                     GDSDDQGIAARMNDFFSGLQDVSTNPGEPAARSVLLANAGQLASTMNQASSDLTTERQ
                     SLNFQVQQTVTEINSLSKQIAELNSQIVSAEATGQNANDLRDQRDLALNDLATRIDIT
                     TVESGTGALTVFAARGQVLVDGSTVRELSAIENPDNDGLFSIGYSTGGTRPLSIDALI
                     SDGRLRSLLDVRDTTVPDLEASFDRLSATLANAVNGIHRQGYGLDGSTGLDFFGPPSV
                     STRAMTANQGAAAINSPAITANSLLTFHDYEIRFSSGSAYSIVDTTTSSVVRGNYTGT
                     AITSPSSDNPLSIVTGTNDTLTVTVDGVASSTITLGGAASPGLAYTSGTTLAQEVQDK
                     INADATLQAAGKTVAVTYDNTTNRMVISSNDTTASSAVNVTGGTARTSLGLSAGTGTP
                     ATGTYTSPATLTFDGLSVTLTGAAAAGDVFKVDSYSHAARDMTVSLTNPASVAASSTR
                     AGIPGNNTNLLSMVALQQQRFASLDDGTLNDAYRAVAGKLGIAAQTADRENEAKETLK
                     DQIETLRAQVSGVSMDEELVNLMKFQRGFEAASRLVKLTDEMFQTLLSIKP"
     gene            5493..>6998
                     /locus_tag="VN647_08400"
     CDS             5493..>6998
                     /locus_tag="VN647_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97087.1"
                     /translation="MRVTELQTFGVLANDLARARARALELQEQVSTGKLVRQPSDDPT
                     AFNHILLDKASLVAIDQWQRNIGFGQTRLNLSDNMLSQASETLSRVQELAVQFRNDTN
                     GAADRTAGAKEVRQLFAQLQQSANTELNGQPIFTGTSTHGRATGLAITTPMTLTNGIN
                     DTLIVTVDGTTSGTIDLTTATESLTGAQLAARVQSRINADTALVNAGKSVTVTFEGDH
                     LLIASDSNGTQSSVRVTGGLARTPLGFTGGSETTGGAPFALTATVASAVGNTGGALAG
                     QGRVVDDNAATLDDYVVRFTSSTTYDVLNVTAPVATSRGTSNVGGAAVLDAGVVNAAQ
                     LTLHNYQIQFTSPTQYSVVDQTTSTTLSSGNTYVSGEAISFAGLRVVLTNGQQGGPQT
                     GDTFSISLSPRSVLSNQPYASGSAISFDGIQVTLSNGTGAPANGDLFSIVSGLQYQGD
                     GGGQAIEIGTGQTVPTNVAGDRAFTAGGEDIFASVKQLIGALRGNDGQGISD"
BASE COUNT         1598 a         1883 c         2040 g         1477 t
ORIGIN      
        1 ttcactgtgg caggaagaga acgggcgggc ttatctctat gaagatctcc gcgcgatgcg
       61 catcggtgac atacttaccg taaagattgc cgaagaccat aaaggctcga aatcagccga
      121 aacggctgtc cagcgagatt cgacgattaa caatagtctt gccgggagcg gcgcggggta
      181 tatcgggatg ccgggacttc gttttagcga tgaaacgaaa cgtcaaatgg gaatcaatgc
      241 gagtgccagt tataaattcg acgggaaagg ggccaccaac cgaacgggga cattaaccgg
      301 cacaatttct gtgattgtga ccgaagttct tcccaacggc gatctcagaa tcgaaggccg
      361 ccgtgaagta acggtaaaca gtgaaaagca gttgatgagc atcggcggca tcgtgcgtcg
      421 agtcgatgtg gataccaaga atacggttct gtcgtccgcc gtcgccgacg ccagaatcga
      481 gtattcaggt ctcggtgtcc tcgacgacgt gcagcgacca ggctggttca tccgcattct
      541 cgattgggtc tatccattct aaagagagga ttgtgctatg gcggtttcgc agaggcgtac
      601 gacaatgagg aggggcattc ccaatcgacg ctccttgaat ctccgaactc tgcagatgat
      661 ggtgcagagt ggagtggtac gacgggctga ttttacccct ccctctttgg gaagcagggc
      721 gcgattccat atgccggatg atatggatcg cggcttgacg aaagaaatag tctctgagca
      781 aggatggttg gcgcgtcttc tacgtggtcg tagggtacag ttgtccgttt ccaaacgttc
      841 gtagcgagga taggcaccga cgcatgatca caatcacacg gcttgccacg atcttcagcg
      901 cgctgatact gaccggttgt ctgttggccc ccccaagtgc cgatgcggtg aggatcaagg
      961 atatcggcgt gattgaaggg gtgcgcgaaa accagctgat tgggtacggg ttggtggtcg
     1021 gtctggatag taccggcgac cgagtgatcg gcggacagtt cacgattcag gcgatgacgt
     1081 ccatgttgaa caaaatgggc gtgaatttgc tggtcaatcc catccagtta ctgaccagga
     1141 acatcgcttc ggtgatggtg accgccaagc ttcctccatt ctcgaaaccg ggaatgacgc
     1201 tggatgcagt ggtgtcgtcg atggcgaatg ccaaaagctt gcaaggcggt acgctcttgt
     1261 ttacaccgct gaaggcagcg aatcagcaag tgtttgcagt tgcacaggga ccggtttcaa
     1321 tcggcggttt tctcggcgga acgggcggcc ctggaggggc cacggtggtc aagaatcatc
     1381 aagcggccgg tctggttccc ggcggagcca tcatcgagaa agaggtgtcg gtcgacatcg
     1441 attcttggga aacagtttct gtactcctgc ggcaacctga tttcacgacc gccatcagga
     1501 cggccgaagc catcgacagc gcttttggga aaggcagtgc atcggccgtg aatgccggat
     1561 ccgtcaaggc cacgattcct caagcgttcc agggccgcat cgtcgaatat attgcatcga
     1621 tcgagggact cgatgtgacc gtcgattcta tagccaaagt cgtcgtcaat gaacggaccg
     1681 ggacagtggt gttgggagaa catgtacgga tttcaacctg tgcgatctct cacggtaatc
     1741 tcacgatctc ggtcaaaaac acgttgaatg tgtcgcaacc gccggccccg cttatcggtt
     1801 cttccgcggg acagaccacc gtcacaccgg atgtgcagac ggagataaag gagcaggaat
     1861 ctcggctcat agtagtggat gaaacagtga cattgggaga ggtggtgcgg gctctcaatg
     1921 cagtgggagt gacgccgcgt gaccttgtcg caattctctc ggctctcaag tccgcaggag
     1981 cacttcaagc tagtcttgaa gtaatttaat catagtattt atttatatgg ttaatgtaga
     2041 taaatatatg agccgatcca cattatctac gccattatat atggaccctg gtttggcccc
     2101 aggttctggt catcctgacc ctcaggtgaa cccctcaaaa tcacagaacg ctgctgaggc
     2161 gcgtcagcaa atggtccagg ccgggcagca gttcgaggcc tatttcatct catatctact
     2221 gaaggtgatg agggaaacgg ttcctgaagg cgccatcacc aataagcaag gggcgtattt
     2281 ttactcattt tacgatgaag agatcggcaa gcgggcggcc gaatccggag gaataggcat
     2341 tgccaggatg gttcaggagt atgcgcaaca atattttgcg tcatctcccg tggaacactc
     2401 aagttcttca ggatagaaac cgataagaac ctcgaccggg gtaaagtcca tacttctctc
     2461 agaatagtcg ctgggagaaa aagccggaga aggagcggca acaatgcagg tttcaggtca
     2521 tgggaaagta gatcatctcg caaagctgtt gcttggggtt caggaaaccg agagttcagg
     2581 ggggcgacaa gcagctgccc gtcctcaggt cggtaaggat gaggtccaaa tttccgccca
     2641 agcgaaggag cttcaacgga ttcgggagct ggccaatcag cctgattccg agcggactga
     2701 acatgtggag cgcatcaggc gcgctcttga cggcggcacg tacgatgtca gtggccgtgc
     2761 ggtcggagat gccctcatga agcaggttct gaccgacgcc atactatagc gtccgccttt
     2821 tcaggctcct gaacactcac tcgccatcat cccttccatc attccgaggg aaagagggga
     2881 ggaggcgacg ggtggcggaa tgcagcgcac gcccatgatc acaaggagtc tgtatgccca
     2941 tgtccaggtt cttgccggga atcatccagc tctgccgctt gttgcaccaa gaggaggcaa
     3001 cctgccatca gctgcttgag acggtccacg cagaacgagc cgccattcgg acgctcgcta
     3061 tcactgagtt ccattccatc aactgccgtc ggctatctat tctagaatcg ttgcagtctc
     3121 ttgccaatga acgacagcac cttgttcaag aggtgggggc gcaccttggt ctccagcctg
     3181 taacgacctc gatccaggaa cttattgaca atgtccaaga ttcggcatcg gaggacttac
     3241 gaaccaccta ccgaagctat atgacggtgg ccaagaccgt ccgcgacgag atcaagcaga
     3301 acgtggtgct catcgaaggt attcgcggcg tggtcgatca agcgctgtcg accggcgcac
     3361 ctgccgttcc aggcggagat ttttatacag cagatggaca atcgttcgtc gcaggtcgtg
     3421 tcaacatgct cattcatcag cagggataat tatgatcggc ctcacatctc tctttgacat
     3481 tgcgagaagc gcgctcacga cctcgcaaca agccctcgtc gtcaccggac agaacgttgc
     3541 caacgtgaat actccgggat attccaggca ggaagccgta cttacagaac gtccgccgct
     3601 gaacggacag cccggcatgg taggggccgg tgtgcaggtc acgtctatca ggcggtatac
     3661 ggaccaattc gtcaaccggc agcttacgac cgtgcagcag aatctcggac ggctgtcggt
     3721 gtcgcaagaa gagttgtccc gcctgcaaaa tctattcgga gacagcgatg atcaaggcat
     3781 cgccgcgcgc atgaacgatt ttttcagtgg tctacaagat gtgtccacca atccgggcga
     3841 gccggcggcc cgttctgttc tgttggcaaa cgccggacag ttggcgtcga ctatgaatca
     3901 ggcttcctcg gatcttacga cggagcgaca atcgctgaac tttcaggtgc agcagacagt
     3961 gacggaaatc aacagcctgt cgaaacaaat cgccgaactg aatagccaga ttgtttcggc
     4021 cgaggcgacg ggccagaacg ccaatgatct gcgggatcag cgtgatcttg cactgaatga
     4081 cttggcgacg cgcatcgaca tcacgaccgt ggagtccggc accggtgctc tgacggtctt
     4141 tgcggcacgt ggacaggttt tggtggacgg atccactgtc cgggagttga gcgccattga
     4201 gaatccggac aacgacgggc ttttttcgat cggctattct acggggggca cgcgtccctt
     4261 aagcatcgac gcactgatct cggatggccg cttacgcagc ctcttggatg tccgtgacac
     4321 cacagtccca gatttggaag cctcgttcga ccgactgtca gcgacgctgg ctaacgccgt
     4381 gaatggtatt catcgacaag gctatggact cgacggatcg accgggctcg atttttttgg
     4441 tcctccgtcg gtatccacgc gcgccatgac cgccaatcaa ggagccgctg cgatcaatag
     4501 ccctgcgatt actgcgaaca gtctactcac gtttcatgac tacgagattc gcttttcctc
     4561 cgggtccgcc tattccatcg tggatacgac gaccagctcc gttgtcagag ggaactatac
     4621 cggcacggcg atcacgtcac cttcgtcgga caatccgctg tctatcgtga cgggtacgaa
     4681 cgatacactc acggtcaccg tcgatggtgt ggcgtccagc acgatcactc tggggggcgc
     4741 tgcaagtccg ggtttggcat acacctccgg tacgactctc gcgcaagaag tgcaggacaa
     4801 gatcaatgca gacgccacgc tacaggcggc cgggaaaacc gtggcggtga cctatgacaa
     4861 tacgaccaac aggatggtca tctcgtcgaa tgatacgacg gcctcatcgg ccgtcaatgt
     4921 gacaggcggc acggctcgaa cgtcgctcgg gttgagtgcc ggcaccggta ctccggcgac
     4981 cggtacatat acaagtccgg cgactctgac cttcgacggc ctcagtgtga cattgaccgg
     5041 cgcggcggcg gccggagacg tatttaaggt ggactcgtac agtcacgctg ctcgcgatat
     5101 gacggtttca ctcacgaatc cggcatccgt tgccgcctct tccacacgag ccggtattcc
     5161 cgggaacaat accaatctcc tttcgatggt cgcacttcag cagcaacgat tcgcgagcct
     5221 ggatgacggc accctgaatg atgcttatcg ggccgtcgcg gggaagcttg gcattgccgc
     5281 acagacggcc gaccgagaaa atgaagcgaa ggaaacgttg aaggaccaga tcgagacgtt
     5341 gcgcgctcag gtgtccggcg tttctatgga cgaggagctg gtgaatctca tgaagttcca
     5401 acgggggttc gaagccgcgt ctcgtctggt aaagctcacc gacgaaatgt tccaaacgct
     5461 gttgtcgatc aaaccttagc gtcgaggctt ctatgcgggt gacagagcta caaaccttcg
     5521 gtgtgcttgc aaatgacttg gctcgggcgc gcgcccgggc tctggaactc caggagcaag
     5581 tgtcgaccgg taaattggtg cgccagccgt ccgatgaccc cactgcgttc aatcacatct
     5641 tgttggacaa agcctcgctc gttgcgatcg atcagtggca acgtaatatt ggttttgggc
     5701 agaccaggct taacctctcc gacaacatgc tgagtcaggc ttcggaaacc ttgtcccgcg
     5761 tgcaagagct tgccgtgcaa tttcgtaatg atacgaacgg cgccgcagat cgtacggccg
     5821 gtgctaaaga agtgcggcaa ttgtttgctc aactccaaca atctgccaat acagaactga
     5881 atggtcaacc gatttttacc ggcacgagca cacacggacg ggccacagga cttgcaatca
     5941 cgacgcccat gactctgacg aacggcatca atgataccct catcgtgact gtcgatggga
     6001 ccacatcagg tacgatcgat ctgacgaccg cgacagaatc tttgaccggc gctcaactgg
     6061 cggcacgtgt gcaaagccgc atcaatgccg atacggcgct ggtcaatgcc ggcaagagtg
     6121 tgaccgtcac atttgaagga gatcacttac tcatcgcatc tgactcgaac ggcacgcagt
     6181 cctcggtgcg tgtaaccgga ggtctggccc gcacgccgct cggatttacg ggaggcagtg
     6241 agacgaccgg cggtgcgccg tttgctctta ctgcaacagt ggcttcggct gtcggtaata
     6301 ctggaggagc tctggccgga cagggccggg ttgtcgacga caatgccgcc accttggacg
     6361 actatgtcgt ccggtttact tcgtccacta cttacgatgt gctgaacgtg acggctccgg
     6421 tggcgacgtc gcgtgggacg tccaacgtcg gtggcgctgc cgtactcgac gccggagtgg
     6481 taaacgcggc acagctcacg ctgcataact atcaaatcca gttcacgtcc ccgacacaat
     6541 actcggtggt tgaccagacg accagcacga ctctgtcatc cggcaacacc tacgtgtcgg
     6601 gcgaggcgat ttcattcgcc ggattgcgtg ttgtgttgac aaacggtcag cagggagggc
     6661 ctcaaacggg ggataccttc tccatcagtc tgtcgcctcg gagcgttctt tccaaccagc
     6721 catacgcatc cggcagcgcg atcagtttcg acggaatcca ggtaacgctg agcaatggga
     6781 caggggcgcc ggcgaacggc gatctgttct ccatcgtctc aggtttgcag taccaggggg
     6841 acgggggggg gcaggccata gaaatcggaa ccggccagac tgtgccgacc aatgtcgccg
     6901 gcgatcgagc ctttactgcg ggtggagagg atatattcgc aagcgtcaag cagttgatcg
     6961 gtgcgttgcg cggcaatgat ggacagggca tcagcgac
//
LOCUS       DATNDD010000348         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3876275, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000348 DATNDD010000000
VERSION     DATNDD010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3876275"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN647_08405"
     CDS             <1..322
                     /locus_tag="VN647_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97088.1"
                     /translation="AGELAMHRHKYDAGITSFTDAVKLEEELPYTEPPFWPIPIRHYL
                     GAALLRAGQPARRKSFTELIWRRIRGTAGLNLDSCRVSVLSGKAAKRTSRKSNGNRHG
                     SMRT"
     gene            587..1132
                     /locus_tag="VN647_08410"
     CDS             587..1132
                     /locus_tag="VN647_08410"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97089.1"
                     /translation="MMRFVVGVMGPAKASKRDLDNARVLGEFIARRGWVVLTGGRDVG
                     VMDAACEGAKRVGGSLTIGVLPTAKDKVSRHVDVPIITEMGSGRNNINVLTSHVVVVC
                     GLSGSGTVSEVALAVKAGKPVILVEASPADVAFFRKLDQRLVNAAASPEEAIELIMKL
                     MDGPQRRAHRADREPFPYLPV"
     gene            1224..1844
                     /gene="msrA"
                     /locus_tag="VN647_08415"
     CDS             1224..1844
                     /gene="msrA"
                     /locus_tag="VN647_08415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWG97090.1"
                     /translation="MAMLRRLTFLAIGLLTCATFALWPSAGLVKASTQAKAYFAGGCF
                     WCMEEAFEKVDGVTAVVSGYMGGTVANPTYEQVSEGHTGHAESIEVTYDPSKVTYQKL
                     LDAFWRNIDPVTPNAQFCDHGTQYRAAVFYSTDEEKRLSEESKSKTEQSKRLPAPIVT
                     QLVQASTFYPAEDYHQDYYKKNPLRYKYYKYSCGRAQRLEALWGKP"
BASE COUNT          473 a          559 c          613 g          420 t
ORIGIN      
        1 ggccggggaa ctggcaatgc accgccataa gtacgacgcg ggcatcacat catttactga
       61 tgccgtcaag ctggaggagg agctcccgta caccgaacct ccattttggc cgattccgat
      121 ccgccactat ctcggtgccg ccttactcag agccggtcaa cccgcaaggc ggaaatcatt
      181 taccgagctg atctggcgaa gaatccgcgg aacggctggg ctcaatttgg actcatgcag
      241 agtctccgtg ctcagcggaa aggccgcgaa gcggacgtcg aggaaaagca atggcaacag
      301 gcatgggagc atgcggacgt gaccctcatc gcctctcggt tttgaggcgc acggcgcgag
      361 gaataacgag cgccatgttt gtgcgcgccg gcgagttggt gagccggcag tgtccgaaac
      421 tcctcacgtt tgcggacggg cgcgaggtcc acgccatacc gaataaggct tgacacgtct
      481 gtgtacgccc gcgagatagt gagcgggccg tgtcccgtga gcgcccagtg tcttcgggcg
      541 acgtgaaaac gaagctggag gacttttcaa catcctgcta ggagaaatga tgcgatttgt
      601 ggttggggtc atggggccgg ccaaagcctc gaagcgggac ctcgacaatg cgcgggtcct
      661 tggagaattc attgcccggc gtggttgggt cgtattgacc ggcggtcgtg acgtgggcgt
      721 catggacgcg gcttgcgaag gggccaaacg ggtgggcgga agcttgacta tcggggtctt
      781 gccgacggcc aaggacaagg tgtctcgtca tgtggatgtg ccgatcatca cggaaatggg
      841 cagtggtcgt aacaacatca atgttctgac cagccatgtc gtggtggtct gtggattgag
      901 cggatcgggg acggtgtccg aggtcgcctt ggccgtgaag gcgggtaaac cggtcattct
      961 tgttgaagcc tctcccgccg acgtcgcgtt cttccgcaaa ctcgaccaac gattggttaa
     1021 tgccgctgcg tctccggaag aagccattga gctgatcatg aagctcatgg atgggcctca
     1081 acggagggcg catcgtgccg accgtgagcc atttccttat ctgccggtgt gaacatcgaa
     1141 tgatacacgc ccaccctctc agcaatcgtc gtagtaagat gtaagaatcg gttcactggg
     1201 acgacgatgg aaaaaggagg atcatggcga tgcttcgacg actgacgttc ctcgcgatcg
     1261 gtttgctgac ctgtgcgaca ttcgctcttt ggccttcagc tggattggtg aaggcatcga
     1321 cgcaggcgaa agcctacttt gcgggcggtt gcttctggtg tatggaggag gcattcgaaa
     1381 aagtagatgg tgttaccgcg gtcgtatccg gctacatggg aggcacagtc gccaatccta
     1441 cctatgaaca ggtctcagaa ggacacacgg gccacgccga gtctattgaa gtgacctatg
     1501 atccgtcgaa agtcacgtac caaaaactat tggacgcctt ttggcgtaac atcgatccgg
     1561 tcacgccgaa cgcgcaattc tgcgatcatg ggactcagta ccgcgcggcg gtcttctatt
     1621 caaccgatga agaaaaacgg ctctcggaag agtcgaaaag caagaccgaa cagtccaagc
     1681 ggcttcctgc accgatcgtg acgcagttag tccaagcgtc caccttctac ccagccgagg
     1741 attaccacca ggattactac aagaagaatc cactccggta taagtattac aaatacagct
     1801 gcggccgagc ccaacggttg gaagcgttat gggggaagcc gtaggagtaa caggagtgag
     1861 caaggaacaa gggaaatttc gaggctatcc caatctccat ttcgaccgat gattcaactc
     1921 cacagccgtt catcggcaca tcccgcacgg tcgccacctc ttcacagtcg gcatgtacaa
     1981 ctttccatcg cactcgacat tgtgcccccc cttgaccctg gcacaatcct ctgccacaaa
     2041 gaacaccggt ccagagggta agtaa
//
LOCUS       DATNDD010000349         5983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_905598, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000349 DATNDD010000000
VERSION     DATNDD010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905598"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VN647_08420"
     CDS             complement(<1..464)
                     /locus_tag="VN647_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97091.1"
                     /translation="MKYSTFQPLSLADFEISPDRGFLPSDPSETLPDCPTLNYLNREM
                     PKLLSTRQVRRFVDEQPSLLPAIPSSWCDDGYRAAMRILSFAGHAYVWETPGNPAIKL
                     PSQLARPWYELAQKLGRPPVLSYVSYALDNWRRLDETKPIQHDNIVLLQNFLG"
     gene            complement(714..1973)
                     /gene="ahcY"
                     /locus_tag="VN647_08425"
     CDS             complement(714..1973)
                     /gene="ahcY"
                     /locus_tag="VN647_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWG97092.1"
                     /translation="MDYDVKDIKLADQGRSKIEWAEATMPVLRLIRKRFKRQQPLKGV
                     RVTACLHVTTETANLAITLKAGGADVRLCASNPLSTQDDVAAALVQHEGIPTFAIKGE
                     DNATYYRHIESAIAHRPHVTMDDGADVVSHLHSKRKELLRNVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSVVVVVGYGWC
                     GRGIAMRAKGMGADVIVTEIDPLKGLEAVMDGFRVMPMEQAAPVGDFFVTVTGNVHVI
                     RGEHFAAMKDGAIVCNSGHFNVELDIPALEKLAGTRRVVRSGVEQFTLKKNGHRVSLL
                     GEGRLVNLATAEGHPSSVMDMSFANQALSAEHIVKNYKQLEKKVYPVPPAIDKEIARL
                     KLAGMGVSIDTLTAEQKKYLASWEMGT"
     gene            complement(2092..3240)
                     /gene="metK"
                     /locus_tag="VN647_08430"
     CDS             complement(2092..3240)
                     /gene="metK"
                     /locus_tag="VN647_08430"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWG97093.1"
                     /translation="MRDNFLFTSESVTEGHPDKIADQISDGILDAIIAQDKYSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYCDASWGFDFHTCSVLTAIHQQSG
                     DIAMGVDSGGAGDQGLMFGYATNETDELMPMPLVLAHRLTKRLAEVRKKNILKWVRPD
                     GKSQVTVEYKNGRPVRIDTIVVSTQHSPDVTTKQLERDIMEKVIKPVMPKGLYDPTGV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPTKVDRSASYMA
                     RYIAKNLVAAGLADKCEVQLAYAIGVADPVSVLVDTKGTEKVSVDILDKLVCKHFPMT
                     PRGIIDHLKLRRPIFRKTAAYGHFGRTEPEFTWEKIDKTKALRKDAGL"
     gene            3564..4118
                     /locus_tag="VN647_08435"
     CDS             3564..4118
                     /locus_tag="VN647_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWG97094.1"
                     /translation="MKFYQAALVFIVMAVSGLCTAPFSEAESPQPKKRPGFEHGVVQV
                     GDEAPNFTLRDLTGNVMSLSQLRGKVVLLNFWATWCGPCRVEMPAMEQLYRTLPRKEF
                     EILAVSTDPQGAAVTRPFQREMGLTFPILHDSEYRVGLTYGARTIPITFMVDRRGIVR
                     QKIFGARDWDSPEARDLIHELMKS"
     gene            4121..4867
                     /locus_tag="VN647_08440"
     CDS             4121..4867
                     /locus_tag="VN647_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HWG97095.1"
                     /translation="MTQSIPQISLIAAFSAGLLSFVSPCVLPLVPSYISYITGLSVEQ
                     LTDASERMKFKKAIVLNSLLFILGFSSVFIAFGASASFFGQLLISHQDLIRRIGGVLI
                     IVFGLYLLGILNLKFLKMEHRFQFRNRPAGYLGSFLIGIAFAAGWTPCVGPVLGSILL
                     YASTTDSLFDGVVLLTFYSFGLGLPLFLTALGVDRFLAYFKEVRAYLWGVSTVSGVML
                     VLVGVMIYANSLTIVTSFLERYGIGWYLGQ"
     gene            complement(4941..>5983)
                     /gene="bamD"
                     /locus_tag="VN647_08445"
     CDS             complement(4941..>5983)
                     /gene="bamD"
                     /locus_tag="VN647_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWG97096.1"
                     /translation="NQSALKRLYHCFFLISLLSLSAMCPQSIRISSSPLSRPSGLCLA
                     AWLGIGSLFMVSCAGDFSPSDVRSGIKKALSGTDEQIFLGDTVENHYHPNVIMKRGEA
                     YFEKEEYAEALVEYKHFMELHRNHVLAPYAAFRIGEIHFKMAKTIDRDPEPMQKAIAA
                     FEQMRKEFSGSRYDSQAQQKLEECHDWIAQMHLFVGQFYYRRGSYLAATHRFQQILKD
                     YPDKPVAAEALYFLAKAYHEMGADDWARENLIVLAEKHPNSTVSEAGKSLLEKIGGAQ
                     PSTLLAQKEESAPLSDAGPEIVRSNRPAVSPSPPPTMGTLGIPPSLDSFGAAAATTLG
                     QGFTVCRIGAWC"
BASE COUNT         1266 a         1576 c         1664 g         1477 t
ORIGIN      
        1 ccgagaaaat tctgcagcaa tacaatgtta tcatgctgaa tcggcttcgt ttcgtcaagc
       61 cgccgccaat tgtccagcgc gtaggagaca taggatagta ccggcggacg gccgagcttc
      121 tgggccagtt cataccacgg acgcgcgagt tgggaaggga gcttgatggc cggatttccc
      181 ggtgtttccc agacataagc atggccggcg aatgaaagga tccgcatcgc ggctcggtat
      241 ccgtcatcac accaactcga tggaatggct ggcaacagcg acggttgttc atcgacaaac
      301 cgccggacct ggcgcgtgct caacaacttc ggcatctcac ggttcagata gttcagtgtg
      361 gggcagtcgg gcagcgtttc actgggatcc gacggcagaa aaccacgatc aggggagatc
      421 tcaaaatctg caagcgacaa cggctggaaa gtggagtatt tcacaacggc tccttgctca
      481 cactctacca ctcctcgaca ggattgcaat caacggttcg taagaatgag catcacgaat
      541 aacaaaggcc gcctcagtag aggcggcttt tcactctctc ctctccaagc aggtgcggcg
      601 tgggtactct cgcactgcgc gcattcaccg agcatcgacc ttgatataga aatcaaatcg
      661 attttgcatg cgcgggagcg agcacagaga gtacccgcgc cgcaccatgc tctttacgtt
      721 cccatttccc aggacgccag atactttttc tgctctgccg tcaatgtgtc gatcgacacg
      781 cccatgccgg ccaacttgag ccgtgcaatt tctttgtcga tggccggagg tacgggatag
      841 accttcttct cgagctgttt gtaattcttg acgatatgct ccgcgctcag tgcctggttg
      901 gcaaagctca tgtccatgac gctggacgga tgcccctcgg cggtggcgag gttgacgagc
      961 cgcccttcac cgagcaaact gacacgatga ccgttcttct tgagcgtaaa ttgctcgact
     1021 ccgctacgga ctacccggcg cgtgccggcc agtttttcga gggcggggat atccagctcc
     1081 acgttgaaat gtccactgtt acagacgatg gccccgtcct tcatggcggc aaaatgttcg
     1141 ccacggatga cgtggacgtt gccggtgacg gttacgaaga aatctccgac cggagcggct
     1201 tgttccatcg gcatcacgcg gaaaccatcc atgacggctt cgagaccttt caacgggtct
     1261 atctcggtca cgatcacgtc ggcccccatg cccttggccc gcatggcgat accgcgcccg
     1321 caccagccgt agccgacaac aacgacgacg gagccgcaga ccaagcgatt cgtggcgcgc
     1381 acgatgccgt ccatggtgga ttgtccggtg ccgtagcgat tgtcgaacat atgcttggtg
     1441 tcggcgtcgt tgacggaaat caccggaaac ttgaggacct tcttttcagc catgctgcgt
     1501 aagcggatga cgccggtggt cgtttcttcc gtgcctccaa tcacgttccg taacagttct
     1561 tttcgtttgg aatgcaggtg ggagacgaca tcggcaccgt catccatcgt gacatgtgga
     1621 cggtgagcga tggcagactc gatgtggcga tagtacgtgg cgttgtcttc gcccttgatc
     1681 gcaaaggtcg ggatgccttc atgttgcacc aatgcagcgg caacatcgtc ctgggtgctg
     1741 aggggatttg aggcacagag gcggacatcg gcgccgcccg ctttcaacgt gatagccagg
     1801 tttgccgttt cagtggtcac gtgcaaacag gctgtgacgc gaacgccctt cagtggctgt
     1861 tgtcgtttaa aacgcttacg aatcaaccgc aacacaggca tcgttgcttc ggcccattcg
     1921 atcttcgatc ttccttggtc cgccagcttg atatccttga catcgtaatc cacgaaagtc
     1981 ctcctcgtcg aaaagaaggc tcaggaaagg tgaggctgag tcagaaaccc aacctctcct
     2041 aagcctgagc ctcagttatt aaaccttaac cttggcctca gcctgtggtc gttacagtcc
     2101 ggcgtctttg cgcaatgcct tggtcttatc gattttctcc catgtgaatt caggctcggt
     2161 gcgaccgaag tgtccgtacg cagctgtctt gcggaaaatc ggccgccgaa gcttgaggtg
     2221 atcgatgatg ccacggggag tcatcgggaa atgcttacac acgagtttgt cgagaatatc
     2281 gaccgatact ttttcagtgc ccttggtgtc gacgagtacg gacaccggat cggcgacccc
     2341 gatcgcatag gccagctgca cctcacactt gtcggccaag ccggcggcca cgagattctt
     2401 ggcaatatag cgggccatat aggaggcgga gcggtccacc tttgtgggat cttttccaga
     2461 aaaggcaccg ccgccgtggc tgccgtgacc accataggtg tcgacgataa tcttccggcc
     2521 tgtaaggcct gtgtcgccca ttgggccacc gaccacgaaa cgaccggtcg ggttgatgtg
     2581 atgtttgacg ccggttggat catagagacc tttgggcatc acgggcttga tgactttttc
     2641 catgatgtcg cgctcaagct gcttggtggt cacatcgggg ctgtgttgtg tggagacgac
     2701 gatcgtatcg atccgcacgg gcctgccatt tttatactcg accgtcactt gggatttgcc
     2761 gtctggccgc acccacttca gaatgttctt cttgcgcacc tctgccaggc gcttggttag
     2821 acgatgggcc aagacgagcg gcatcggcat gagttcatcc gtttcattgg tggcgtaccc
     2881 gaacatcaag ccctgatcac cggcccctcc ggaatccacc cccatcgcga tatcaccgga
     2941 ttgctggtga atggcagtga gaaccgagca ggtatgaaag tcgaatcccc atgaagcatc
     3001 gcaatagcca acgtccttga tgacgtcacg aataatgtcc gggatttcaa cataggcctt
     3061 ggtggaaatt tctcccgcta caaaagcgat gccggtagtc aggatcgttt cgcaggcgac
     3121 gcgggaatat ttatcctgag caatgatggc gtctaagatg ccgtcggaaa tctggtcggc
     3181 gatcttatcc ggatgtcctt cagtgaccga ctccgaagta aacaagaaat tgtctcgcat
     3241 gtgcccctca tgggttcagg ttatggaaca tcagcgttgc tgtgtgctga tggtgaatgt
     3301 gacgagtggt gtcgagtgac agaccacgca caaacctggg caattctaga gagatcgatc
     3361 ggctttgtcc atcacttcga tcgtaggggg aggaaatttg tcacacaccg tttgcttgac
     3421 tcaggttttt gtggacttgt aacatacctt catagcaaga gaatctgcca atggctgaga
     3481 taaggagctt gatcatagtg gccacggcca ttttgacggc taagcatttt gacagctaag
     3541 aatgtgtcta tctgtgtatt ggcatgaagt tttatcaagc ggctttggtt ttcatcgtca
     3601 tggcagtgag tgggttgtgc acggcaccct tttccgaggc agaatcaccc caaccaaaga
     3661 agcggccggg cttcgaacat ggtgtcgttc aagtaggtga cgaagcgccg aatttcacgc
     3721 tgcgcgactt gaccggtaat gtcatgagtt tgtctcagct cagggggaag gtcgtcctcc
     3781 tcaatttctg ggcgacctgg tgcggaccgt gccgtgtcga aatgccggcg atggaacagc
     3841 tttatagaac gttgccgcga aaggagtttg aaatcttggc ggtgtcgact gatccacagg
     3901 gcgcggcggt cacccgtccg ttccagcgag aaatggggct cacatttccc attctgcatg
     3961 attcggaata ccgcgtgggc ctgacgtacg gggcccgtac gattccgatc acgttcatgg
     4021 tcgatcgccg aggcatcgtg cgacaaaaga ttttcggtgc acgtgattgg gactcaccgg
     4081 aggcccgtga cttgattcat gaattgatga agtcgtagcc atgacgcaat ccatcccaca
     4141 gatttcactc attgccgcct tttcggcggg gctcctttca ttcgtgtccc catgtgtgct
     4201 gccgttggtg ccgagctata tttcctacat taccggccta tcggtcgagc agctgacgga
     4261 cgcgtccgaa cggatgaagt tcaagaaggc gatcgtcttg aactcgttgt tgtttattct
     4321 cggattttca tcggtcttca ttgcgttcgg tgcctcggcc agctttttcg gtcagctcct
     4381 gatttcccat caagacttga ttcggcgcat tggtggtgtg ttgattatcg tgttcgggct
     4441 gtatctgctt gggattctga acctgaagtt cttaaaaatg gagcatcggt tccagttccg
     4501 caataggccg gccgggtacc taggatcgtt cttgatcggt atcgcgttcg ccgccggttg
     4561 gactccttgt gtcggaccgg tgctggggtc cattcttctc tatgccagta cgaccgactc
     4621 tctttttgat ggagtcgtgt tgttgacgtt ttattcgttc ggattagggc tgccgctgtt
     4681 ccttacggca ttaggcgtcg atcggttctt agcctatttc aaagaagtgc gggcctatct
     4741 gtggggtgtt tcgactgtga gtggcgtcat gctggtcctt gtcggcgtga tgatctacgc
     4801 caattcgctc accatagtga caagtttctt ggagcgatac gggatcggct ggtatctagg
     4861 ccagtagata gtatcagcag gcggtggtgt aagacattcc gggcgaccgt tgctttactc
     4921 agcactcatt cctatttcaa tcagcaccaa gcaccaatgc ggcagactgt aaagccttgt
     4981 cccagtgtgg tggcagcggc agcgccaaac gagtcgagtg acggaggaat gccgagcgtt
     5041 cccatggttg gtggtggaga cggactaaca gcgggccgat tgcttcggac tatctccggg
     5101 ccggcatcgg aaagaggagc cgattcctcc ttctgggcta agagcgtact tggctgggca
     5161 ccgccgattt tctccagcaa actctttcca gcctctgaga ccgtactgtt cggatgtttt
     5221 tccgccaata cgatcaggtt ctcccttgcc caatcgtcgg ctcccatttc atggtatgcc
     5281 ttagcgagaa agtacaaggc ttctgcggcg acgggcttat caggataatc ctttaagatt
     5341 tgctggaacc gatgcgtggc ggcaagatag gagcctcgcc ggtaatagaa ttgtcccacg
     5401 aagagatgca tttgagcgat ccaatcatgg cattcctcca gtttttgctg agcctgacta
     5461 tcgtaccggc tgccggaaaa ttcttttctc atctgctcaa atgcggcgat ggctttttgc
     5521 atcggttcag gatcacgatc gatggtcttc gccatcttaa aatgaatttc tccgattcga
     5581 aatgctgcgt acggggccag gacgtgattg cgatggagct ccatgaaatg tttatactcg
     5641 actaatgcct ccgcatactc ttctttctca aaatacgctt ccccccgttt catgatgacg
     5701 ttgggatggt agtggttctc aaccgtatct ccgagaaaaa tttgttcatc ggtgccgcta
     5761 agggctttct tgattccgct tcgtacgtcg ctgggtgaga aatcaccagc gcacgagacc
     5821 atgaacagag agccgatgcc gagccaagca gccaggcata agcctgatgg ccttgataac
     5881 ggactggaac tgattcgaat cgattgagga cacatggcgg atagagatag caaggatatg
     5941 agaaaaaagc aatgatacag acgtttgaga gcgctttggt tga
//
LOCUS       DATNDD010000350         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3330609, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000350 DATNDD010000000
VERSION     DATNDD010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330609"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..238)
                     /locus_tag="VN647_08450"
     CDS             complement(38..238)
                     /locus_tag="VN647_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97097.1"
                     /translation="MIEKKPYSMPQLFRVDLDQEQAILSACSLMTTSVSQGGNGGCRP
                     NNAGGCKRRNGPGRTDSGPRAS"
     gene            complement(235..1365)
                     /locus_tag="VN647_08455"
     CDS             complement(235..1365)
                     /locus_tag="VN647_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWG97098.1"
                     /translation="MGESRIISRVSYGTFSEQIHARSTQHQQVVKAQLELTYRCNLHC
                     RHCYTDPYNNPEFLPRELTRDELYHLLAELQDLGVIWLNLSGGDIFMHPQFFDVYEQA
                     YRRGFLLQLYTNGTLFTRATIQRLQTMPPFTIDVSCHTVNEARFDWFTQVPGSYRAFL
                     RGMTLLRESGLPFTLKTKAMNWNKDELDDLRTFTESFGQSFGYTTSLSPRLNGDCSSL
                     AYRISPEDLLVIGQKEPTSEQGETCHAELPLGRPETDRLFRCGCGTNTIHINAWGELS
                     TCTFQYETRVSLRQISLREAVARVFAQVQNLRYQRDTPCRSCTVHSFCDKQPSHARWE
                     MEDRQAPIPYDCDVAMTRAESVLHQTLVHPLKQCSQGEQPPS"
     gene            complement(1358..>2257)
                     /locus_tag="VN647_08460"
     CDS             complement(1358..>2257)
                     /locus_tag="VN647_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWG97099.1"
                     /translation="GKLRAFPNRFPLSCQWEITCRCNLHCVMCYTDCRNRPEFVRQEL
                     TTTEILRIMDDMGAAGTLELCLSGGEPMGRTDFFVIYEQAVRRGFLVTVFTNGTLIKE
                     VEADRFAALPPHGIEISLHGSTAGTFERITQHQGSYQRCLRAIRLLCDRHLPLTLKAT
                     VLSLNQHEILAIKEYAASLGPVRLKLGDELRPELDGGAGPFRYAVTEQELTELHGKDP
                     LLWIEACRRAQAELPPCRSGMQRFHIDAYGRMQLCSGNRRQSYDLRTGSFREGFYEAL
                     PMFTCEHKQPEIAPLLQPMVHHG"
BASE COUNT          503 a          604 c          632 g          518 t
ORIGIN      
        1 gtatccgccg atccgaacag tcagatccat attgctccta cgacgcacga ggaccggaat
       61 cggtgcgacc tggtccattc cgtctcttgc agccgcccgc gttgttcggc ctgcacccgc
      121 cgttgcctcc ttgcgaaaca ctggttgtca tcaggctaca ggcgctcaaa atggcttgtt
      181 cctggtccag atcgacacga aagagttgtg gcatgctgta cggtttcttc tcgatcatga
      241 tgggggttgc tctccttgtg aacactgctt caagggatga accagcgtct gatgcagtac
      301 cgattcagcc cgtgtcatcg ccacgtcgca gtcatacggg atcggcgctt gtcggtcctc
      361 catttcccat cgggcgtgtg atggctgttt gtcgcagaaa ctgtgcacgg tacaggatcg
      421 gcaaggcgtg tcgcgttgat agcgcaggtt ctgcacctgt gcaaagactc gtgcaaccgc
      481 ctcacgcaga gaaatctgcc tgagcgacac gcgggtttca tattgaaacg tgcatgtgct
      541 gagctcgccc caggcattaa tgtgaattgt gttggtaccg caaccgcagc gaaacagccg
      601 atcggtttcc ggccgaccga ggggcaattc tgcgtgacag gtttcgcctt gttcggatgt
      661 cggttctttc tgtccaatca ccagtagatc ttcaggcgaa attctgtagg ccaaagaaga
      721 gcaatcgccg ttgagtcgcg gcgacaacga ggtggtgtac ccgaacgact gtccgaacga
      781 ctcggtgaac gtccgaaggt catcgagctc gtctttgttc cagttcatcg ctttggtttt
      841 gagcgtgaac ggcaagccgc tttctcgaag caacgtcata ccccgaagaa atgcacgata
      901 cgagccgggt acttgggtga accaatcaaa ccgtgcctca ttcaccgtgt ggcacgagac
      961 atcgatcgtg aagggaggca tggtttgcaa tcgctggatc gtcgcgcggg tgaacagcgt
     1021 cccattggtg tatagctgga gtagaaaccc tcgccgatac gcttgttcgt acacatcaaa
     1081 aaactgaggg tgcatgaaaa tgtcgccgcc cgacagattc agccagatga cgccgaggtc
     1141 ttggagctcc gccagcaagt gatacagttc atcgcgcgtc agttcgcgcg gcagaaactc
     1201 aggattattg taaggatcgg tgtaacaatg acggcaatgc agattgcagc gataagtcaa
     1261 ttcgagctga gcttttacaa cctgctggtg ctgcgtcgat cgagcatgga tctgctcgct
     1321 gaacgttccg tacgaaactc tggatatgat tcgcgactca cccatgatgc accatcggtt
     1381 gaagcagtgg tgcgatctct ggttgtttgt gttcgcaggt aaacatcggc aacgcttcgt
     1441 aaaatccttc tctgaacgaa cctgttcgca aatcatagct ctggcgtcga tttcctgagc
     1501 agagttgcat ccggccataa gcgtcgatat ggaaccgctg catcccgctc ctgcaggggg
     1561 gaagctcggc ctgtgctcgt cgacaagctt ctatccaaag caacggatcc tttccatgca
     1621 actctgtcaa ttcttgttca gtcactgcat aacgaaaggg accggcccct ccatcgagtt
     1681 ccggccgaag ctcgtcaccg agcttgagtc ggaccggacc gagcgaagcg gcatattctt
     1741 tgatcgcgag gatttcatgt tggttcagtg ataggacggt cgccttcagc gttagtggaa
     1801 gatggcgatc acacaacaaa cggatcgctc tgagacagcg ttgatacgaa ccctggtgtt
     1861 gggtgatccg ctcgaatgtt ccggccgtcg agccatgcag actgatttcg atcccgtgag
     1921 gaggcagagc tgcaaatcga tcggcttcga cttctttaat caacgtcccg ttcgtgaaga
     1981 cagtgacgag aaagccgcgc cgaacggctt gttcataaat cacaaaaaaa tcggtcctgc
     2041 ccatcggttc accgccggac aggcacagtt ccagtgtgcc ggcagcgccc atgtcgtcca
     2101 tgattcgaag aatctcggtg gtggtgagct cctgacgaac gaactccggt cgattgcggc
     2161 aatccgtgta acacatgaca cagtgaagat tgcaacgaca ggtgatctcc cattggcagg
     2221 agagtgggaa gcggttgggg aaggcgcgaa gcttacc
//
LOCUS       DATNDD010000351         6793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3012643, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000351 DATNDD010000000
VERSION     DATNDD010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3012643"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VN647_08465"
     CDS             complement(<1..1288)
                     /locus_tag="VN647_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWG97100.1"
                     /translation="MKARLLGSFFKVVNVNSWKTYFVVVLGLMLCVSGNESAQATTGV
                     GPYEVPQVVDINADPSIVETYIVADEATVNIGNGVTANAMTFKSCADPAVPATCSTTG
                     TIPGPEFRLKVGDQVIVHFVNNLNPTKVDPKDTEANVSGIHWHGIELNNQSDGTELTQ
                     AAVVPGGTFDYKFTVSRPGIFWYHPHHHSSTNQVAKGLYGSIIVEDKYGYEAALIAKS
                     VIPSASQTKTLVISDITVCSPGNNPATFNGDLPHVSGIQSWEINYLNHPLTQSPSILC
                     DADPLDANGNVRGPFNVGDIPNIQSPGLIGRMSEGFTVLTNGVNVGARAGTPAAPGVL
                     ANGAQTLDVQAGQGLRLQIVNPSPVRYVRLRLTTGEEKINLVRIGGEGGLLDHALVEG
                     GITAGDFDTGYGRGEILLPPAGRADVVAKIPSTVAQN"
     gene            complement(1794..2543)
                     /locus_tag="VN647_08470"
     CDS             complement(1794..2543)
                     /locus_tag="VN647_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG97101.1"
                     /translation="MSCKNRFDEASLQQIPRAIRLMEQSIRIRERARVVLIDDSSIVL
                     KGLQMALSKSSRIQVAGTARTEDEAVALLKRCQPDVAVLDVQVGQSSGINLCGVIRES
                     YPKTAVLFFTANDDKYTLRSAILAGAQGYLLKGASQEAIVKSVEIVAAGKAIMDCRLT
                     QQLLAWVRDGRQPAQHDRMEDCTRADLQLLSLIAAGKSNKEMAQELNVTPSAVTARLR
                     AVYKRLHVSRRSEAVRYFVQWEKDSLAHGPF"
     gene            complement(2765..3454)
                     /locus_tag="VN647_08475"
     CDS             complement(2765..3454)
                     /locus_tag="VN647_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWG97102.1"
                     /translation="MKNAGTMVPSLSLTIISNQYFGWLVLQKIFKCVEDIRIIVQLHL
                     KMTPAVLLAERQPDVFILDMETEQDPVGAIRQIRESASKSKVVVLSGFTDTDRTREAF
                     EYGVDGVILKIQPAAVVLAAIKALYSPAYNHAPVRPCDAVSMDLRATTGQDADPDAQS
                     SVWPKGLTERKREIIRLVGQGLSNKDIADRLCISDSTVRHHLTGIFNKIGVPNRQKLL
                     IHAHRFSFSAV"
     gene            3829..4035
                     /locus_tag="VN647_08480"
     CDS             3829..4035
                     /locus_tag="VN647_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97103.1"
                     /translation="MEGWDAGSEYKPKQKYELENERFYRCLLAEWSTLLRQVPGMEVI
                     QMNAVDFNVSVRGNNQLSVNKLLS"
     gene            4032..4232
                     /locus_tag="VN647_08485"
     CDS             4032..4232
                     /locus_tag="VN647_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWG97104.1"
                     /translation="MIDGRSIYIDQSGTVFWKQLPVALTEIKRIEVLKGPASVVYGFN
                     AFDGVVSIITKSPEEMKEPRCR"
     gene            4229..4714
                     /locus_tag="VN647_08490"
     CDS             4229..4714
                     /locus_tag="VN647_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97105.1"
                     /translation="MTGGEVGTLLTSAVHTGTTGKWGYRLLGGHEQTQRWSHRDTPAL
                     NGQRIGGIAEYHLSGDGKIRTEAGLARSSPYNDIVHQTAAANIHVSQTYALISYEQSG
                     LLIRGWWNGLFSDTDTRLFPPLSPLLTLTDRFGRTDQESSLNTYDAEIRYRFSPLETS
                     I"
     gene            4872..5399
                     /locus_tag="VN647_08495"
     CDS             4872..5399
                     /locus_tag="VN647_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97106.1"
                     /translation="MRTRLTGFFNHISDLIAFRTLPNAPLNTVRPLNGGVADVYGGEV
                     GAEFLLTSWLSRFANYAYQEVGQTSTGFSRRGFPHHKVNAGLRATWSPFTGEVLYHYV
                     RDASYPLADVFTSLAPVFPAETVLPQELAPSYNLLNMRLGYLIRRQQASDSCGKPSWP
                     FPCSMRSTIGTASTL"
     gene            5590..6552
                     /locus_tag="VN647_08500"
     CDS             5590..6552
                     /locus_tag="VN647_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWG97107.1"
                     /translation="MPPVFFILSLFLFTHLSGIAGAEEIAVLKSAGLPYYEQAVVGFK
                     AGIPATTSVKEYNLNGQLTEGRQIAKSLRASPPDLIFAVGLKAAMAAKLEIFDQPVVF
                     CMVLNPETHGLPAPNMTGIAVRLPVETQLAALRSALPNHNRVGVLYDEDHSSDFIRQA
                     HRAAKQQGLELLAISVHRQEEIPNVVRALLPKIDALWLIQDQTVVSESAIPFFLESTL
                     DAKVPLFTFSSTLVQQGALGALVLDPWAVGQQAAHIALARLSGRHASTGLLQNPERPQ
                     LALNLNSAEHLDLVLPAELIRMAGHLFGGPGALAQQPDQSDLIP"
     gene            6593..>6793
                     /locus_tag="VN647_08505"
     CDS             6593..>6793
                     /locus_tag="VN647_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97108.1"
                     /translation="MVELAPPSPSFSITLRTKLVLSTTVILIVACLLLGWLFIQQQVR
                     SAAESLVQSGTLLAQYLAGMGRF"
BASE COUNT         1547 a         1911 c         1728 g         1607 t
ORIGIN      
        1 agttttgcgc gacggtactc ggaatcttcg ccaccacatc cgcacgacct gcaggcggga
       61 gcagaatttc gccgcgccca tatcccgtat caaaatcacc agccgtaatg cccccctcaa
      121 caagcgcgtg atcgagcagc cccccttctc caccgattcg aaccagattg attttctcct
      181 caccggtcgt caaacggagg cgaacgtagc gcaccggcga gggattcacg atctgcaacc
      241 gcagtccctg acctgcttgg acgtcaagtg tctgggcacc gtttgcaagc acgcctggcg
      301 ccgcaggagt tcccgccctc gcaccaacat tgacgccgtt ggtcaggacc gtaaaccctt
      361 cactcatacg accaatcaac ccgggagatt gaatgttcgg aatgtctccg acattgaatg
      421 gacctcgtac attcccattt gcatcaagcg gatcagcatc gcagaggatg ctcggggact
      481 gagtcaaagg atgatttagg tagttgatct cccacgattg aatgccgctg acgtgcggca
      541 gatcgccatt aaatgtggcc gggttgttcc caggactgca taccgtgatg tcgctgatca
      601 ccagcgtttt ggtttgcgat gccgaaggga tcacgctctt cgcgatgagg gcggcctcat
      661 acccatattt atcttcaaca atgatcgacc cgtagagtcc tttggccact tgattggtcg
      721 acgagtggtg atgcgggtgg taccagaaga tccccggccg gcttacggtg aacttgtagt
      781 cgaaggtacc ccctggaacc actgcagcct gggtcagttc cgtgccgtca ctttgattgt
      841 tcaactcgat tccgtgccag tgaataccgg atacattcgc ctctgtgtct ttaggatcga
      901 cctttgtcgg attgaggtta ttcacaaaat gcacgatgac ctggtctccc accttgagcc
      961 gaaactccgg acccggtatg gtcccggtcg tagagcacgt agcagggaca gcagggtctg
     1021 cacagctctt aaacgtcatc gcgttcgccg tcaccccatt gccgatattg acggtcgcct
     1081 catctgccac aatgtaggtc tcaacgatgc tgggatcagc attaatgtcg acgacctgag
     1141 gaacctcata cggcccgact ccagtcgtgg cctgcgccga ctcgttgccg gacacgcaca
     1201 gcatgagccc caagacaacg acaaaatacg ttttccaact gttgacattc acgactttga
     1261 aaaaactccc tagtaaccgt gctttcatgg tccatctccc tcgttatttc ccatcatccg
     1321 ctcgaactat tacggcgaaa tccttcctga catgtcactg gtccagactt cgcccggttg
     1381 gtgacacgtg acgtaggtgc gtccatattt catcggtctc cagatcttgg acaggggtcg
     1441 tccgacgcac gtgccagttc tatgccaaca agttacgact gaacatcatc ctagcttttg
     1501 aaatggaggt aatacttctt caatcgaaaa ggacggacaa ctcatagtct tggagcggta
     1561 tttgtgatct gcgtgttgtg atggtcttca ctctccaaca agatcacaag gtgtgcagaa
     1621 ttatcttgca ggtacccctg tatccaaata ggttcagtat ccaattcgat tcatctcatc
     1681 ggagcaggag gcttaaaaaa gcatgagaca ttgaagtcac ctttttggta ttcgtacaaa
     1741 gcactcatat gcatgatcgt tcagaaatac aaccaatcag cgtggaggtt tactcaaaac
     1801 ggtccatgcg ccagggagtc cttttcccac tgcacaaagt acctcaccgc ttccgatctc
     1861 ctcgagacat gtaaccgctt gtagaccgcc cggagacgag ccgtaacagc actgggggtg
     1921 acgttgagct cctgtgccat ctctttattg cttttcccgg cagcaatgag tgacaggagt
     1981 tgcagatcgg ctcttgtgca atcttccatc cgatcgtgtt gtgccggctg ccttccatcc
     2041 cggacccacg cgaggagctg ctgcgtcaat cgacagtcca tgatggcctt accggctgcg
     2101 acgatttcta cactcttcac tatggcttcc tgagacgccc ccttcagcaa gtaaccttgt
     2161 gctccggcaa gaatggcaga ccgcagggtg tacttgtcgt cattggcggt gaagaagagg
     2221 acggcggttt tcggatacga ctcacggatg accccacaca aattgatgcc gctcgattgc
     2281 cctacttgta catccaagac ggcaacatcc ggctgacatc tcttcagaag ggcaacggct
     2341 tcatcttcgg tgcgtgccgt gccggcgacc tggatacgac tgctcttcga cagggccatc
     2401 tgtagccctt taagtacaat actcgaatcg tcgatcagaa ccacacgagc tcgttctcgt
     2461 attcgaatcg actgctccat caaacggatg gctcttggga tttgctgaag agacgcttca
     2521 tcgaatcggt tcttacagga cacaacggag cctagattca aagttccttg caatcgtgca
     2581 agtggcattc cagatagaga aatttgtgaa ataggccgca acgacgcact acgttccaat
     2641 gtgcttttac cgtacagtaa tcagttgcta taccgaacga gacaggcaac ttactttctt
     2701 ggcagcatgg ttactgaggg acgcgagctt cgacgcaacg ttctgataaa gaactccggc
     2761 tagtttagac tgcggagaag ctgaagcgat gggcgtggat caggagcttc tgccgattgg
     2821 gcacaccgat cttattgaaa atgccggtga gatgatggcg gaccgtactg tcggaaatac
     2881 acaaccggtc ggcaatgtct ttgtttgaga gtccctggcc gaccaatctg ataatctctc
     2941 gtttgcgttc agtcagcccc ttgggccaca cagacgactg tgcgtcggga tcggcatctt
     3001 gtccggtggt tgccctcaga tccatgctca ccgcatcaca aggtctaaca ggagcatggt
     3061 tataagcggg ggaatacagc gccttgatcg cagcgagcac aactgcagca ggttgaatct
     3121 tcagaatgac gccgtcaacg ccgtattcaa aggcctcccg cgtacgatcc gtgtccgtga
     3181 acccactcag caccacaacc ttacttttcg acgccgactc cctaatctgc ctaatcgcgc
     3241 cgacggggtc ttgttcggtc tccatatcaa gaatgaacac atctggttgt ctctctgcga
     3301 gtagcacagc aggtgtcatc ttcaggtgca gttgcacgat aatccgtata tcctctacgc
     3361 atttgaagat tttctgcaat acgagccacc cgaaatactg attactgatg atcgtgaggc
     3421 tgagcgatgg caccattgtg ccggcgtttt tcatgaaatc tccttaacca agacgcatga
     3481 ctgagtgtcg aaaagcttct ctaatgtgat gttcatctcc cagcctacga gacatgctcg
     3541 gcaaatggat tttgtgagtg atatgcgatt gtgtttcgac gctctctcac gactcgtact
     3601 cgtcgatcaa ataacattcc actgcagata tgtcttttga cgagcctgaa tcgaagccgc
     3661 tcatcacttg atggtgaagc gctgcaatga gattcaggag ctgagtgaaa ccgaatagga
     3721 atgagagtga gtgaagtttc tcccatcact gtatctaaat ggattctgca tctaattcga
     3781 ttcacctgaa gacgaagcac cggcggcgaa ttccattcct caacaattat ggaaggctgg
     3841 gacgcgggca gcgagtacaa gccgaagcag aagtatgaat tggagaacga gcgtttttat
     3901 cgctgcctgt tggctgagtg gtccactcta ttgcgacaag tcccgggtat ggaagtcata
     3961 caaatgaacg cggtggactt caatgtcagc gtgcgcggaa ataatcagtt gtcggtgaac
     4021 aagctcctct cttgatcgat ggccgctcca tctatattga tcaatccggc actgtattct
     4081 ggaaacagct gccggtcgcc ttgacggaga tcaagcgaat cgaggttctg aaggggccgg
     4141 catcggtcgt gtacgggttc aatgccttcg acggggtggt gagcatcatc acgaaatccc
     4201 ctgaagagat gaaggaacca cgctgcaggt gacaggtggt gaagtcggca ctctgctcac
     4261 cagcgctgtc catacaggaa ccacggggaa gtggggctac cgtttattgg gcggacatga
     4321 gcagacacaa cgttggtctc atcgcgacac accggcgctc aatggtcaac ggattggtgg
     4381 gatcgccgaa tatcacctgt caggcgatgg aaagatcagg accgaggccg ggctcgcacg
     4441 atcgagccct tacaacgata tcgtgcatca aaccgccgct gcaaatattc acgtttccca
     4501 gacctatgca ctgatcagct acgaacagag cgggctgttg atccgtggct ggtggaacgg
     4561 cttgttctct gacacagaca ctcgcctatt ccctccgctg agtccgctgc tcacgctcac
     4621 cgacagattt ggacgaacgg atcaggagag ctccctgaac acctatgatg ctgaaatacg
     4681 gtaccgattc agtccgcttg agacctcaat ttgaacatgg gggccaactt tcgccacatc
     4741 atttattcaa cgaacattct cagcaaccgg acggccgaga accgtcttgg actttatgcg
     4801 cacggagatt ggcaggtgtg gccttctcta gcgatcagcg ccgggctccg ttacgacctt
     4861 gataccttcg gctgagaacc agactcaccg ggtttttcaa ccacatttca gacctcatcg
     4921 cattccgaac cctgccgaac gccccattga acactgttcg tcctctcaac ggcggtgtgg
     4981 ctgatgtata cggtggggaa gtcggcgcgg aattcctact gacatcgtgg ctctccaggt
     5041 tcgccaacta tgcgtatcaa gaagtcggac aaacctccac cggattcagc cggcgaggtt
     5101 ttcctcacca caaggtgaac gccggactcc gggcaacctg gagcccattc acgggcgagg
     5161 tactctatca ctatgtgaga gatgcgtcgt atccgttggc cgacgtgttc acaagtttag
     5221 ctccggtttt cccggccgag accgtgcttc cccaagaact ggcccccagc tacaacttgt
     5281 tgaatatgcg acttggctat ctcattcggc gacaacaggc ttctgattcg tgcgggaagc
     5341 cgagctggcc atttccgtgt tcaatgcgct caacgatcgg caccgcgagc accctttagg
     5401 cgatttactc ggcactcgag tgatgggctg gctcacaatg aagctttgat tcactcgtca
     5461 cctacagttc tgtggcccaa gagttgcttt actatttttg gctcactgca cacctatagt
     5521 gcaggtatcg atgttccagg gcacttgaga atgttagtat agccagcggt tgtcggaggg
     5581 aggcccttca tgccgccagt attctttatt ctctctctct tcttgttcac acatctctcc
     5641 ggcatcgccg gcgccgaaga aattgccgtc ctcaaatcgg ccggccttcc gtactatgag
     5701 caagccgttg tcgggtttaa agcaggaatc cctgcgacca catcggtcaa ggaatataac
     5761 ctcaacggcc aactcacaga aggacggcaa atcgccaagt ctttgcgggc ctctcccccg
     5821 gatctgatct tcgccgtcgg gcttaaggct gccatggccg ccaagctgga gattttcgac
     5881 cagccggtgg tgttctgcat ggtactcaac ccggaaacac acggactccc tgcccctaac
     5941 atgaccggaa tcgccgtccg actgcccgta gagacacaac tcgcagcgct tcgctccgcc
     6001 ctacccaatc acaatcgagt cggggtcctc tatgatgaag atcacagcag tgactttatc
     6061 cgccaggcac atcgagcagc caaacagcag gggttggagc tccttgcgat ctccgtccat
     6121 cgccaagagg agattcccaa cgtcgtccga gcattactcc caaagatcga tgcactctgg
     6181 ctgatacaag accagaccgt ggtatccgaa tcagccatcc cgtttttctt ggaatcgacg
     6241 cttgatgcca aggtgccgtt gttcaccttc tcctcgacac tggttcagca aggagcgctc
     6301 ggtgcactgg tcttggatcc gtgggctgtg ggacagcagg cagcccatat cgcgctcgct
     6361 cgccttagcg gacgtcatgc gtcaaccggt ttgctccaga atcccgaacg ccctcaattg
     6421 gcgttgaact taaactcggc agagcacttg gacctcgttc tgcctgccga gctgatacgt
     6481 atggccggtc acctgtttgg aggtccggga gcgctggccc agcaaccgga tcaatccgat
     6541 ttgatccctt agaggggata acttgtagct gacagtcgat acaggcacgg caatggtcga
     6601 actggctcca ccctccccct cattctccat cacgctcagg acgaagctcg tcctcagtac
     6661 gaccgtcatt ctgattgtcg cctgtctctt attaggctgg ttgtttattc aacagcaagt
     6721 gcgatcggct gctgaaagcc tggtgcaaag tggaacgttg ctcgcgcaat acctggcggg
     6781 catgggtcga ttc
//
LOCUS       DATNDD010000352         6978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1304640, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000352 DATNDD010000000
VERSION     DATNDD010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304640"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /gene="gatA"
                     /locus_tag="VN647_08510"
     CDS             <1..1185
                     /gene="gatA"
                     /locus_tag="VN647_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWG97109.1"
                     /translation="MLQNFIPPYDATTVAKLRAQEYLLVGKTNLDEFAMGSSTENSAF
                     GPSRNPWNLHCVPGGSSGGSAAAVAAEECVAALGSDTGGSIRQPAAFCGVVGLKPTYG
                     RVSRYGLVAFASSLDQIGPITRDVPDAAFLLQAIAGHDPMDSTSVDRSVPDYMKSLQK
                     RDLKTLKVGVPVEFFTEGLDSDVEQAVRGAIGELKNLGAQIKEIRLPSTGAAVAVYYV
                     IATAEASSNLARFDGVKFGFRAKETKDLLELYMKTRQEGFGPEVKRRIMLGTYVLSAG
                     YYDAYYGKAQAVRTLVCQDFAAAFKEVDLIVTPATPTPAFKLGEKSEDPLQMYLSDIF
                     TISVNLAGLPAIALPCGFSKTGLPIGLQLIGRAFEEETVLRAAHAYEQSTHWHLKKPM
                     IR"
     gene            1237..1644
                     /locus_tag="VN647_08515"
     CDS             1237..1644
                     /locus_tag="VN647_08515"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HWG97110.1"
                     /translation="MTIVEIAALLVAVAFAVLVGYLVPVLMQVRKTVAESEQLLSKMN
                     AEVPALVAELRAMSQNLNDVTNQAREGVEHAAVLLHAVGEVGESVQQVHNVVRGSGGT
                     LLNRVASMIAGFRAASRVVRERMKQEGGTHNGG"
     gene            1634..1957
                     /locus_tag="VN647_08520"
     CDS             1634..1957
                     /locus_tag="VN647_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWG97111.1"
                     /translation="MADDRGTSAMVFLAFLSGATMGAVAALLLAPQSGDESRDQIRRY
                     ARRAENDLRDLAGRAGEAFEEVVDQGKEFVETKRSVLRDAFDAGREAVKRERGRIHDE
                     GPERG"
     gene            1954..3381
                     /gene="gatB"
                     /locus_tag="VN647_08525"
     CDS             1954..3381
                     /gene="gatB"
                     /locus_tag="VN647_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWG97112.1"
                     /translation="MTYETVIGVEVHAQLRTKSKMFCGCGTTFGLSANSQTCPICLGL
                     PGSLPVINRTAVDMAVRAGLALNCTIAANNQFARKNYFYPDLPKGYQISQYESPICQH
                     GWIEIHDSSGKKRIRIRRAHLEEDAGKNIHETGTGGSRVDLNRAGTPLLEIVTEPDMG
                     SADEVVAYLKGLREILMYLDVCDGNMEEGSFRCEPNLSLRPSGQKEFGTKVELKNINS
                     FKYVKDAVEYEIKRQTKVLNEGGTIRQETRLWNIERGETAVMRSKEEAHDYRYFPDPD
                     LVPLKLDDEWIEGFRADLPELPAVRTKRFVSDYGLPEYDATILTASKGMADYFEVCAK
                     QFTQPKTVSNWVMGELMRELNNSGTDISASPVTPERLVSLLQMVGQGTISLKVAREIF
                     PELYSSGKTPEQIVQEKGLTQVSDEGALAKIIEEALSKNPGQVEQFKDGKQQVLGFLV
                     GQVMKASGGKANPGKVNELLKKRLG"
     gene            3577..4863
                     /gene="eno"
                     /locus_tag="VN647_08530"
     CDS             3577..4863
                     /gene="eno"
                     /locus_tag="VN647_08530"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWG97113.1"
                     /translation="MSAIREIRGRQIIDSRGNPTIEAEVTLESGAKGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVSNISKVIAPELLGKEAFDQAGIDRAMIALDGTKT
                     KSKLGANAILGVSLAVAKASANETGQPLYRYLGGSNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIMPVGAGRFSEALRMATEVFHTLKALLKKKGLNTAVGDEGGFAPDLQSNEEA
                     LGLISQAIEEASYKVGQDIALALDCAASEFYDKGRYVLEAEKNPERSSDEMISYYGKL
                     LDRYPILSIEDGLSELDWKGWKILTEKLGKRVQLVGDDIFVTNVEIFSKGIKEGIGNS
                     ILIKLNQIGTLTETLDAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTGS
                     LSRTDRVAKYNQLLRIEEELGAVALYRGREAVTSRA"
     gene            4891..5250
                     /locus_tag="VN647_08535"
     CDS             4891..5250
                     /locus_tag="VN647_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWG97114.1"
                     /translation="MIIKQNRGRQWLKWRQRILIVMQVIGAGGCVWLFVALFSGEMGL
                     TRYLSMRDHASNLEQELSALHRENAILQRDITRLQHDPAKIEQLAREQLGYVRKGETV
                     YQLAPESKKKPEPFSKP"
     gene            5202..6131
                     /gene="era"
                     /locus_tag="VN647_08540"
     CDS             5202..6131
                     /gene="era"
                     /locus_tag="VN647_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWG97115.1"
                     /translation="MSARVQEKTGALLETMKFGTVAIIGRSNVGKSTLLNRLLGEKIS
                     IVSSKPQTTRTRIMGVAHVEGAQIAFLDTPGLHKPEHLLNRRMVRTAVETMEEADLFY
                     MLMDATSLPSPGDLTAVKHMKEALAKQPRPVILVVTKIDLVNKQKLLPILDRYGKLFA
                     WTEVVPISAQVDDNVDRLLAVTVPYLPSGEGAYGDDVVTDQTIRTLAAEIIREKVLQK
                     TEEEVPYAVAVEIDEFVEQGKLAKIRASILVERETQKGILIGKQGKRLKAIGTQARLD
                     MERLFDVKVFLELWVKVRKAWREDEQTLVQLGY"
     gene            6142..>6978
                     /locus_tag="VN647_08545"
     CDS             6142..>6978
                     /locus_tag="VN647_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG97116.1"
                     /translation="MQAERPIEPRVPDQRPRQSERDVLREILRDQTERGQTKSDIVIQ
                     SVQKLLRRGAITNLSKMLGRMHPADIAKVVTHLSSPKEKREIFELVRGEGKRGQALSE
                     LDGESIQQVLADLLHADIAWLLKDLGPDDVAYILGCLPEERSKEILALMKTEDSTEVA
                     DILKYPKDTAGAIMTTEFFSLPEDATAQEAIRRLQQATDAEMVFYIYVTDKDDRLVGV
                     LSLRQLITVPPTTPLKNIMTREVMSVAIDMDQEEVARQVASYNLLAIPVVERDGKLVG
                     IIT"
BASE COUNT         1697 a         1634 c         2199 g         1448 t
ORIGIN      
        1 atgttgcaga acttcatacc gccttacgat gcgacgaccg tcgccaagtt gcgtgcgcaa
       61 gagtacctat tggtgggtaa gacgaattta gatgaattcg cgatgggatc gtcaacggaa
      121 aactccgcgt tcggtcccag ccgaaatccc tggaacttgc actgtgtgcc gggaggatca
      181 agcggaggat ctgcggcggc ggtggcggct gaggaatgtg tggcggcact tggctcagac
      241 acaggcgggt ccatcaggca gccggcagcc ttctgcggcg tggtgggact gaagccgact
      301 tacggacggg tctcaagata tgggttggtt gccttcgctt cctcgctgga tcaaatcggg
      361 ccgattacga gagacgtgcc tgacgcggca tttcttctgc aggccattgc cggtcatgat
      421 ccgatggatt ccacctcggt cgaccgttcc gtgccggatt acatgaaatc gctgcaaaaa
      481 cgggatctca aaactctgaa ggtcggcgta ccggtcgaat tttttaccga aggactcgac
      541 tcggacgtcg aacaggcagt cagaggggcc attggggagt tgaagaatct tggcgctcag
      601 atcaaagaaa tccgtctgcc gagcaccggc gcggctgtgg ctgtgtacta tgtgatcgcg
      661 acggcggaag ccagctcgaa tctcgcccgc ttcgacgggg tgaagttcgg ttttcgtgcc
      721 aaggaaacca aagatcttct cgaactctac atgaagacac ggcaggaagg gtttggaccg
      781 gaagtgaagc gtcgaatcat gctggggacg tatgtgctca gcgccgggta ttacgatgcc
      841 tattatggga aggcccaagc tgtgcgaacg ttggtctgcc aagatttcgc ggccgcgttc
      901 aaagaagtgg atttgatcgt gactccggcc acgccgacgc ccgccttcaa gcttggtgaa
      961 aagagcgaag atcccttaca gatgtatctc tccgatattt ttacgatttc ggtcaacctg
     1021 gcaggattgc cggcgatcgc cctgccgtgt ggtttcagca aaacagggct tcccattggc
     1081 ttacagctga tcgggcgagc ttttgaggaa gagacggtgc ttcgggccgc acatgcctat
     1141 gagcaatcga cacactggca tctcaaaaaa ccgatgatac ggtagaggca gccgaggaag
     1201 aacattcgag atgaaagacc gggaggattg gcaactatga cgatcgtcga aatcgccgcg
     1261 cttctcgtgg cggtggcatt tgctgtgctg gtcgggtatc tcgtgccggt attgatgcaa
     1321 gtccgtaaaa cggtggccga gtctgaacag ctcctctcga agatgaacgc cgaggtgccg
     1381 gctctcgtgg ctgaactgcg ggccatgagc cagaatttga acgatgtgac gaaccaggcg
     1441 cgcgaggggg tcgagcacgc ggcggtcttg cttcacgcag tgggtgaagt cggtgagtct
     1501 gtgcaacagg tccataacgt cgttcgcgga tcgggaggga cgctcttaaa cagagtggcg
     1561 agcatgattg cgggtttcag ggcggcctct cgcgtcgtgc gggaacgcat gaaacaagaa
     1621 ggagggacac acaatggcgg atgatcgagg aacatcagca atggttttct tggcattttt
     1681 aagcggagcg acgatgggcg ccgtcgcggc gctcttgctg gcgccgcaat cgggggacga
     1741 atcacgtgac cagattcgaa gatatgcccg tcgcgcggaa aacgatctgc gggaccttgc
     1801 aggacgggcc ggtgaggcat ttgaagaagt cgttgatcag ggcaaggaat ttgttgagac
     1861 gaagcggtcg gttttgcgag atgcattcga tgccgggcgc gaagccgtga agcgcgaacg
     1921 cggtcgtatt catgacgaag gtccggagcg cggatgacct acgaaacggt cattggagtg
     1981 gaagtgcatg cacaactgcg gacgaagtcc aaaatgttct gcgggtgcgg cacgacattt
     2041 ggtctgtcgg ccaacagcca gacctgtcca atttgtctcg gcctaccggg cagtttgccc
     2101 gtgatcaatc gaacggcggt cgacatggcg gttcgtgcgg gtttagcact gaactgtacg
     2161 atcgcggcga acaaccaatt cgcgagaaag aactatttct acccggattt accgaaaggg
     2221 tatcagattt cccaatatga gtccccgatt tgccagcatg ggtggattga gattcacgat
     2281 agtagcggga aaaaacgaat ccgtatccgt cgcgcgcatt tggaagagga tgcggggaaa
     2341 aatatccatg agaccggcac cggtgggagc cgggtagatc tgaaccgcgc cggtacaccg
     2401 ctattggaga tcgtgacgga gccggatatg ggttctgctg acgaggtggt cgcttacctt
     2461 aaagggctgc gggagatctt aatgtacctt gacgtctgcg acggcaacat ggaggaagga
     2521 agcttccggt gtgagccgaa tctgtcgctg cgtccgtcgg ggcagaagga gtttgggacg
     2581 aaagtcgagc tgaagaacat caactccttc aagtatgtga aggatgcagt cgagtatgag
     2641 atcaagcggc agacgaaagt gttgaatgag ggaggcacga ttcgtcagga aacgaggctc
     2701 tggaacatcg aacgtggtga aacggcggtc atgcgttcga aagaagaagc ccatgactat
     2761 cggtacttcc ccgaccctga tctggtgccg ttgaagttgg acgatgagtg gattgaagga
     2821 ttccgggccg acttgcccga actgccggcc gtgcggacga agcgatttgt gtcggactat
     2881 gggttgcctg aatatgacgc caccattctg acggcttcga agggcatggc ggactacttt
     2941 gaagtttgcg caaagcagtt cactcaaccc aagaccgtga gcaattgggt gatgggagag
     3001 ttgatgagag agttgaacaa ctccgggacg gatatttcag cgtcacctgt cacgcctgaa
     3061 cggctggtga gtcttttgca gatggtgggt caagggacca tcagtttaaa agttgcccgt
     3121 gaaatctttc cggaactcta tagcagtgga aagactccag agcagattgt gcaggaaaaa
     3181 gggttgacgc aggtctctga cgaaggagcg ctggcgaaga tcatcgaaga agccctcagc
     3241 aagaatcccg ggcaagtcga gcagttcaag gatggtaagc agcaagtatt gggcttcctc
     3301 gttggacagg tgatgaaggc gagcggcggg aaagcgaatc cgggaaaggt gaatgaatta
     3361 ttgaagaaga ggcttggatg atcaggggtg gggaacgacc aggtagactt ctgtgctcgc
     3421 gcaacgcgcc cgcaaagccc tatcaaatgt cgatcccagt gagtggtgct cgttgcatgc
     3481 gcgcagttgg agtccacctg gtcatccccc atcatctagc ggcaaagaga tcttaactgg
     3541 ttagaacgat cgattgaaat aggaaggtga gagagcatga gcgcgattcg agaaatcagg
     3601 ggtaggcaga ttatcgattc acggggcaat ccgacgattg aggccgaagt gacgctcgaa
     3661 agcggcgcga aggggcgggc ggccgttcca tcgggagcgt caacgggaga aaaagaagca
     3721 atcgaactcc gcgacggtga taagaaacgg tggatgggaa agggagtctc aaaggcggtc
     3781 tcgaatatca gtaaggtcat cgcgcccgaa ctacttggca aagaggcgtt tgatcaagct
     3841 ggtattgatc gagccatgat tgcattggat gggacgaaga ccaagagtaa gctgggagct
     3901 aatgcgatct tgggtgtctc gttagctgtt gcgaaggcgt cggcgaatga aacagggcaa
     3961 ccgctctatc gttatctcgg aggatcgaat gctcgagtgc ttcccgtccc gctcatgaac
     4021 atcatcaacg gcggagccca tgctgataat cgcctggacc ttcaagagtt catgatcatg
     4081 ccggtaggtg caggccggtt cagcgaagcc ctccgaatgg ccaccgaggt ctttcataca
     4141 ctcaaggcgc tcttaaagaa aaaggggctc aacacggctg tgggggatga aggtgggttc
     4201 gctccggatc tgcaatcgaa cgaagaagcg ttaggtttaa tctctcaggc gattgaggag
     4261 gcaagctata aggtcgggca ggatatcgcc ttggcgttgg actgcgcggc gagtgagttc
     4321 tatgacaagg gacggtatgt tcttgaagcg gaaaagaatc cggaacgttc gtcggatgag
     4381 atgatcagct actatggaaa gttgctggat cgttatccga tcctctcgat tgaggacggg
     4441 ttgagtgaat tggattggaa ggggtggaag attctcacgg agaagctggg caaaagggtg
     4501 cagcttgtcg gcgacgacat cttcgtcacc aacgtggaaa tcttttcaaa gggcatcaag
     4561 gaaggaatcg gaaattcaat tctgattaag ctcaatcaga tcgggaccct gacggaaacc
     4621 ttggatgcga tcgaacttgc gaagcgatcg ggttatacgg cgatcatttc gcaccggtcc
     4681 ggtgagactg aagataccac gatcgcggac gtggcggtcg caacgaacag tggattgatc
     4741 aagacggggt ccttgtctcg aacggatcgc gtggcgaaat ataaccaatt gctccgaatc
     4801 gaagaagagc tgggagccgt ggcgctttat cgtggtcgag aggcagtgac gtccagggcc
     4861 tagctggtac gtagtcggtg ggtggataga atgattatca agcaaaatcg tggacggcag
     4921 tggctgaaat ggcgacagcg catactgatc gtcatgcagg tgatcggcgc tggcggctgt
     4981 gtgtggctgt tcgtggcctt gttttccgga gaaatgggcc tgactcggta tctctcgatg
     5041 cgtgatcacg ccagcaattt agagcaagaa ctctcggctt tacatcgaga aaatgccata
     5101 ttgcagcgag atattacccg tctccagcat gaccccgcca agatcgagca gttggcacga
     5161 gaacagttgg gctatgtgcg caagggcgaa accgtgtatc aattagcgcc agagtccaag
     5221 aaaaaaccgg agcccttctc gaaaccatga agttcggaac ggtggccatc attggccggt
     5281 ccaacgtcgg caagtcgacg ctgctcaatc gcctgcttgg cgaaaaaatt tcgattgtat
     5341 cgagtaagcc gcagaccaca aggacccgca tcatgggagt cgcgcatgtg gaaggagcac
     5401 aaattgcctt tctcgataca ccggggcttc ataaacccga gcacctgctg aatcgtcgaa
     5461 tggtgcgcac tgccgtggag accatggaag aggcggattt gttctacatg ctcatggatg
     5521 cgacgagtct gccgagtccc ggagatttga ccgcagtgaa gcatatgaag gaggcgttgg
     5581 ccaagcagcc gcgcccggtc attcttgtcg tcacgaaaat cgatctcgtc aacaagcaga
     5641 agttgctccc gattcttgat cgttacggaa aactctttgc gtggacggag gtcgtgccga
     5701 tttccgccca ggttgacgac aatgtggacc gattattggc cgtgacagtc ccttatcttc
     5761 cgtccggaga aggagcctac ggcgacgatg tcgtgacaga tcagacaatc agaacgctgg
     5821 cggctgagat aattcgtgag aaggtgttgc aaaagacgga ggaagaagtg ccgtatgcgg
     5881 tcgcggtcga aatcgatgag ttcgtcgagc agggcaaatt ggcgaagatc cgggcgtcga
     5941 ttttggtgga gcgagaaacg cagaagggca tcctcatagg taagcaagga aagcgtttga
     6001 aagcgatcgg tacgcaagcc agactggata tggaacggct attcgacgtg aaagtgttcc
     6061 ttgaactgtg ggtaaaggtg aggaaggcgt ggcgtgagga cgagcagacc ttggtgcagt
     6121 tggggtattg acggaacgga tatgcaggcg gaacgaccga tagagccacg ggtaccggac
     6181 cagcgacctc gccagtccga acgcgacgtg ttgcgggaaa tcttgcgcga tcagaccgaa
     6241 cgcggtcaga cgaagtcgga cattgtgatc cagtcggtgc agaagttact gcgacgcgga
     6301 gccatcacca acctctccaa aatgttgggg cgtatgcatc ccgcggacat cgccaaagtc
     6361 gtgacccatc tctcttctcc gaaggaaaag cgtgaaattt tcgagttggt ccgcggcgaa
     6421 ggcaagcgcg gacaagcgct cagtgaactc gacggcgaaa gtattcaaca agtactcgcc
     6481 gatctgttgc atgccgacat cgcgtggctc ttgaaagatc tcggtcccga tgatgtggcg
     6541 tacattctcg gatgccttcc cgaagagcgc agcaaagaaa tcctcgcttt gatgaaaacc
     6601 gaggattcga ccgaggttgc cgatatcttg aagtacccga aggatacggc aggcgcgatt
     6661 atgaccacgg agttcttttc cttgccggag gatgccactg cacaggaagc gattcgtcga
     6721 ttacagcaag ccaccgatgc ggaaatggtg ttctacattt atgtaacgga caaggatgac
     6781 cgcctggtcg gtgtcctctc gcttcggcag ctgatcaccg tccctccgac gactccgctg
     6841 aaaaatatca tgacccggga ggtcatgagt gtggcgatcg acatggatca agaagaggtc
     6901 gcgcgccagg tggccagcta taatttgctg gctattccgg ttgtggaacg ggacggaaaa
     6961 ctcgtcggca tcatcacg
//
LOCUS       DATNDD010000353         2460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2711921, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000353 DATNDD010000000
VERSION     DATNDD010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711921"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VN647_08550"
     CDS             complement(<1..255)
                     /locus_tag="VN647_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWG97117.1"
                     /translation="MPPFKLEAPFIPCGDQPEAIARLTAGVQSGKRHQVLLGVTGSGK
                     TFTMANLVERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAV"
     gene            complement(380..679)
                     /locus_tag="VN647_08555"
     CDS             complement(380..679)
                     /locus_tag="VN647_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWG97118.1"
                     /translation="MGAKIYVGGLPYSTTEQQLSDLFATHGAVASARIITDKFTGQSR
                     GFGFVEMSSDAEAQAAITALNGTQLGGRTLTVNEARPQEPRSGGGGRGGFGGGRG"
     gene            complement(942..1733)
                     /locus_tag="VN647_08560"
     CDS             complement(942..1733)
                     /locus_tag="VN647_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97119.1"
                     /translation="MLEKYRTCSVIRRVQTGLVTLLLACLGLVTAYTPSYGVQKETQE
                     EKAANLKKQATGGLFHRWTFDQDQANTQPGSFVKGISTDESLADWIVQDHATVPSPPN
                     VIAGSSSCTQLCYQVLLANGLEYEYPDLSVRFHAQEGTSGLGGIVFGVRDTRNFYAAI
                     VDPSGQKVQLVRISNGSELLLAQASISLKKVDWHSLRVQRNTIISKDFIEVFADGVLV
                     LSVEDQALGSGQIGLVVLGSSTVLFDSFHAVPLFSHRPLSAPPAY"
     gene            complement(1765..2016)
                     /locus_tag="VN647_08565"
     CDS             complement(1765..2016)
                     /locus_tag="VN647_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97120.1"
                     /translation="MAGGESGKGLYDHLYRRFFEQRDSHDGYSFQQAPGGSAPTPVVT
                     FGEKLRWWTWDRWQRRKKILAERDRLQREILQIKRSGSS"
     gene            2189..>2460
                     /locus_tag="VN647_08570"
     CDS             2189..>2460
                     /locus_tag="VN647_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97121.1"
                     /translation="MIFICLLSDSMVRCTVVLGAAFLLLPLTSTSSLAQSPETVLQTA
                     IDSLSAERMLADIRTLSGPSFNGRQSGTKADLESARWVAQEFLSAG"
BASE COUNT          578 a          734 c          605 g          543 t
ORIGIN      
        1 gacggcgtta tgggggaaaa actgtttgaa ctcttgatag agttgaccgg ccaacgtctt
       61 gttatggacc aacacgagcg tcggcttctg gacacgctcg acgagattcg ccatcgtgaa
      121 ggtcttgccg gaccccgtca ctccgagcaa tacttggtgt ctcttcccgg actgtacccc
      181 agcggtcaat cttgcaatgg cttccggctg atcgccacaa ggtatgaagg gagcttctag
      241 cttaaacggc ggcacagcac tctccattgg tgcatgaaca gatatccgat gaacaggttg
      301 ctcttggcgg ttcgcgttaa aagtaaaaaa gggccgccgg aaattccggc agcccttatc
      361 gcagggaaca gacctgagat taaccgcgac cgcctccgaa tcctccacgc cctcctccac
      421 cagaacgggg ctcctgagga cgagcttcgt tcacagttaa cgtacgacca ccgagttgtg
      481 taccgttcaa ggcagtaata gccgcctgcg cttccgcatc ggacgacatc tcgacaaatc
      541 caaatcctcg agactgtccg gtgaacttgt ccgtaataat cctcgccgag gccacggccc
      601 catgcgtcgc aaacaggtcg ctcagttgtt gctcagtcgt tgaataaggc aatccaccga
      661 catagatctt cgcacccatc ggactcctcc tttgacacaa tgatgttgtc ttggactcga
      721 gaaagcaacg aggaaggaat gggccgaaga cgtcaacaca gcggcgactt agctctggct
      781 tccgatcaga ttcccgggct ggcccaacac aacatccgtt caggccttgt cactttcagc
      841 accggcattg ccaggccttt cgcaaaccgg ctacgctatg ctactccaga ccggcgaaaa
      901 aaggcaagtt cgaggccaaa tcggctcgct ggtgagcttc gctaataggc tggcggcgcg
      961 gacagcggcc ggtgggagaa gagaggaact gcatggaagc tgtcgaacaa cacggtagat
     1021 gacccaagaa cgaccagccc aatctgccca gatcccaagg cttgatcttc gacggaaagg
     1081 acaagcactc catccgcaaa cacttcaata aaatctttgc tgatgatggt atttcgttgc
     1141 accctgagcg aatgccagtc aacctttttc agactaatcg acgcctgcgc gaggagtaat
     1201 tcgcttccat tcgagatgcg aacgagctgc accttttgac cgctcggatc gacgatcgcc
     1261 gcgtaaaaat tgcgcgtatc gcgcacgcca aacacgatgc ctccgagccc ggacgttcct
     1321 tcctgtgcat gaaagcgaac gctgagatcc ggatattcat actccaatcc attggccaac
     1381 aacacctggt aacagagttg cgtacaactg gatgagcccg cgatgacgtt tgggggagaa
     1441 ggcaccgtgg catgatcctg tacgatccaa tctgcaagcg actcgtcagt agaaataccc
     1501 ttaacgaagc tgccgggttg agtattggcc tgatcctggt cgaaggtcca cctatggaaa
     1561 agtccacccg tcgcttgctt cttcagattg gcagcctttt cttcctgtgt ttccttctga
     1621 actccgtaac taggggtgta cgccgttacg agacccaggc aggccagcaa gagagtgacg
     1681 agcccggtct ggactcgcct gatgacggaa cacgttcgat atttctccaa cacagacagt
     1741 ccctgaatac acagtccctg aatactatga gcttcccgat ctcttaattt gaagaatctc
     1801 tcgctgcaga cggtcccgtt cggcaaggat tttctttcgt cgttgccatc gatcccaagt
     1861 ccaccaacgc agcttctcgc cgaacgtcac tactggtgtc ggggcactgc cgccgggagc
     1921 ctgctgaaac gaatagccgt catggctgtc acgttgctcg aaaaatcgcc gatacaaatg
     1981 atcgtacagt ccctttcctg attcaccacc agccatgctc acccctctac tccagaaaca
     2041 gtgtcagtcg ctcctaacat taccgaacat gtcacagcca cctactgccc gccgaatagt
     2101 acctgggctc aatgcagctc tgcaacctgc tttccctcat gctatgatcc cacatctgaa
     2161 tgcaaacctg acgtgatcag gtatcactat gatttttatc tgtttgctca gcgattccat
     2221 ggtccgttgc accgtggtcc tcggtgccgc cttccttctt ctcccactga cgtctacgtc
     2281 aagcctggct caatcgcctg agacagtcct tcagacagcc atcgattcgc tctccgcaga
     2341 acggatgctt gccgacatcc gaacgctcag cggcccctcg tttaatgggc ggcaaagcgg
     2401 cacaaaagca gacctcgaat cggcgcgatg ggtggcacaa gagtttcttt cggcaggctt
//
LOCUS       DATNDD010000354         3805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3013826, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000354 DATNDD010000000
VERSION     DATNDD010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013826"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VN647_08575"
     CDS             complement(<1..537)
                     /locus_tag="VN647_08575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG97122.1"
                     /translation="MGRYIVITPARNEELFIEKTAESMIAQAAKPTAWIVVNDGSTDL
                     TTELVEKYKARHSFIKLINVERSGDRNFRNKVHAFNQGLEQARRFEHDFIGNLDADIS
                     FDSDYFQRLLAEFQRDSTLGLAGGMVASYIDGAFVKQEVSLDSVAGAVQLFRRSCFEQ
                     VGGYVALPLGGIDAAAEIM"
     gene            complement(1535..1720)
                     /locus_tag="VN647_08580"
     CDS             complement(1535..1720)
                     /locus_tag="VN647_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97123.1"
                     /translation="MAKVIMELGGWGYLVLVLAWYSEKIVRCYVGLQAKAELWLLAVE
                     QSPAQQFPQGVRHQGSS"
     gene            complement(1965..3026)
                     /locus_tag="VN647_08585"
     CDS             complement(1965..3026)
                     /locus_tag="VN647_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HWG97124.1"
                     /translation="MKRKKIWIDLDNSPHVPLFVPIINELEKAGHSVILTARDTFQVL
                     GLADYYGLKYAIVGRHYGANKLLKVIGTLWRSLQLAAVILKKNPDLSLSHGSRALILL
                     SWCLRIPTILMFDYEHVTFLPLIKPTLGVAPESINDSRIASYFKRGLRLYSGIKEDIY
                     VPNFRPDSSILQQLNLRDSDIIVTIRPPASEAHYHNPDSDKLFAKVVDALGQTPGMIR
                     MVILPRNEKTQKEFIYKAWAAWCKDGKITIPDRVIDGLNLIWHSDLVISGGGTMNREA
                     AALGIPVYSIFRGPLGAVDKYLAENGRLVMIETQADLETKIRLVKRPKEPDKGMDGRM
                     TLKQVMNAIEEVLNDVALS"
     gene            complement(3062..>3805)
                     /locus_tag="VN647_08590"
     CDS             complement(3062..>3805)
                     /locus_tag="VN647_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97125.1"
                     /translation="KYCKVVSADDWIESDCLAQMVRVAEAYPTAAIIGSYQRCGGKVL
                     GEGLPSNVEFLSGREVSRLSLLSLGFPKTLDAFGTQTSVLYRSDVIRKNDPFFPHLFP
                     YADTTACYKYLQHHDFGFVHRALSIMRLHDDTVSSKIADKYGMGLVGSLEHIIEYGPV
                     FLEKNELEGLENRFLRKYYKSMGANLLKLNWERKVWSYQISRMQELGYRISFWKLIKG
                     AAEEVAEELHDPRMAFRKFFRALERRVSR"
BASE COUNT          899 a         1059 c          894 g          953 t
ORIGIN      
        1 catgatctcg gctgcagcat caatcccacc gagtggcaat gccacatacc ctcccacctg
       61 ttcaaagcaa ctccgtctga acagctggac cgcccccgcc acgctatcga gtgagacttc
      121 ctgcttcaca aacgctccgt caatatacga agcgaccatc cctcctgcta acccgagagt
      181 ggagtccctc tgaaactcgg caaggagcct ttgaaaataa tcggagtcaa atgagatatc
      241 tgcgtcgaga ttaccgatga agtcatgctc aaagcgcctt gcctgttcga gcccctgatt
      301 aaacgcatgc accttgttcc tgaaattgcg gtcgcctgag cgctcgacat tgatcaactt
      361 aatgaaacta tgtcgtgcct tgtatttttc aactagttct gtcgtcaagt cggtggaccc
      421 atcattcacg acaatccacg ccgtcggctt ggcagcctga gctatcatcg actcggcggt
      481 cttctcgatg aacaactctt cattacgcgc gggggtaatt acgatgtatc tccccatata
      541 tcatgtgccc ctaaaaatgc acttctattt tccagaacat ctccttcagt ggtatcagct
      601 tcgccaacct ggtaggccgg gaggagagaa gattcgagag ggccagatgc ctccttcctc
      661 ggttctcccg ccaattcctt ggtttcgaag aaccgccaag atccgcgtgc ggttttccca
      721 gtgcaaagtc tgaaatctta tcgtttcatt ggaagtcctc tcggaagcat ccatactttt
      781 tccttcaatt aatccgggta tgagactcgt aacagtccaa gaacttcatt gagatttggt
      841 ggaccacggc gcgacggaac ttgtaccgat cctgatgact ctcatcaata gatggcttca
      901 cattcgtcag ctcggcaggg cactgggcag agcggctcac aggaaggttt ccagatcaag
      961 aagtccgttg agaacagccc ctcgttcggg aaaaggcctg gtccgggaag attccagctt
     1021 taccgaacta tgccgctcca ctatagacgt ttagcagctg attccgccgg ccttatcttc
     1081 caatcggtct gccctacgcc tacgcaaggt tgcggatagc ggtcttcgag aatgagccag
     1141 cacactgact cttctaccag tgtgaggaag ccggcgatga cgcctgcttc ctccctaagt
     1201 tgctggttgc aggcccccca tttgtcaaac ggccaccaac tgagtgatgt gaccgctgag
     1261 tggttgcttg acctgacacg gtgtccggca gccgcgtgct gaatgcaagc accgggtgtt
     1321 gtgtcattcg accctgtcga tgagcctttg ttccggttcc aggtacttgt ccacccccgg
     1381 agctggaaca gcatttcttt gagaggccgc atgagccgtt ccgtgtcgac gttctcttcg
     1441 gattgctgta gctgatgagg agtctgcgtg atatccagcg ttgcgtagac cttcgggaaa
     1501 acgaccgacg tgggctgacg tccgtgctcg ctcatcagct ggagccctgg tgtcgcacgc
     1561 cctgcgggaa ctgctgggcc gggctctgct ccaccgccaa cagccagagt tcggccttgg
     1621 cttggagccc gacatagcac ctgacgatct tttcgctata ccaggcgagc accaggacga
     1681 ggtagcccca gccacccagc tccatgatca ccttcgccat atcgatgcca caccattggt
     1741 tggacactgt cgggcagcgt ttgctgcacg tcaggctccg tatcgccttc aagtaggaat
     1801 tgctcgtgac cggcaacggt agtcgcgtat tagtcgacgg atgagcttct tattgacgga
     1861 caccccctcc gcaagccggg gtgagcctag atgcggcgat atctccagaa tgaatggtcg
     1921 gcttatagcg cctgataccc gatactccat cctccgaggc cggcctacga tagagcgaca
     1981 tcatttagta cttcctcgat ggcgttcatt acttgcttca aggtcatgcg gccatccatg
     2041 cctttatccg gttctttagg tctcttgacc aatcgaatct tcgtctccaa atccgcctgt
     2101 gtttcaatca tcaccagacg cccgttttca gctaaatact tgtctaccgc tccaagaggg
     2161 ccccgaaaaa tactgtaaac agggataccc aaagcagccg cttccctgtt catagtaccg
     2221 cctccgctaa tcaccagatc cgaatgccag attaaattaa ggccgtcgat aactcgatca
     2281 ggaatggtta ttttcccatc cttacaccac gcggcccagg ctttatatat gaattccttt
     2341 tgagtctttt catttcgcgg caatataacc attcgaatca tccctggagt ctgacccaaa
     2401 gcgtcgacga ccttggcaaa gagcttgtcg ctatcgggat tatgatagtg cgcctcactg
     2461 gcaggcggac ggatcgtgac gatgatatcg gagtctctga ggttgagttg ctgcaagatg
     2521 gaggaatcag gccgaaagtt aggcacatag atatcttctt taataccaga atacaaacgc
     2581 aagccacgct tgaaatagct ggctatacga gagtcattga tgctttcagg ggcaacaccc
     2641 agtgtcggct tgataagtgg gagaaatgtt acgtgctcat agtcaaacat gaggatcgtc
     2701 gggatacgaa gacaccacga gaggagtata agggcgcgcg acccatggga tagtgacaga
     2761 tccgggtttt tcttcagaat caccgctgca agttgtaggc ttctccatag agtaccgatc
     2821 accttcaaga gcttattggc cccatagtga cgccccacta ttgcatactt taatccatag
     2881 taatcggcaa gaccgagcac ctgaaatgta tctcgtgccg tcaaaataac cgaatgccct
     2941 gctttctcta gttcattgat gattggaaca aagagcggca catgcggaga attatcgaga
     3001 tctatccaaa tctttttgcg tttcatgaac ggcttcactc ggtagaagct tcagcatcct
     3061 ctcatctact aacccttctt tccagagccc taaagaactt ccggaatgcc attcgtggat
     3121 catgcaattc ctctgcaact tcctccgcag cgccttttat tagtttccag aagctgattc
     3181 gatatcccag ttcttgcatc ctggaaattt gataactcca aaccttcctc tcccagttta
     3241 atttcaacag attcgccccc atacttttgt agtactttct caggaatcta ttttctagtc
     3301 cttccaattc gtttttctca agaaacacag gcccatactc aatgatgtgc tcgagggatc
     3361 cgaccaaccc catcccatac ttgtcagcaa ttttcgaaga gactgtatca tcgtgaagac
     3421 gcattatgga aagggcacgg tgtacaaatc cgaagtcgtg atgctgaaga tacttgtagc
     3481 atgcggttgt atccgcgtac gggaaaagat gagggaaaaa tggatcgttc ttcctgatca
     3541 cgtcagaccg atacaagacg ctggtttgag tcccaaaagc atccaatgtc ttggggaatc
     3601 cgagcgacag caaactcaat cgagagactt ctcggccgga caggaattca acgtttgagg
     3661 gcagcccttc accgagcacc tttccaccgc agcgttgata cgacccgatg attgcagcgg
     3721 tcggataggc ctctgccact ctcaccattt gagccaggca gtctgattca atccaatcgt
     3781 ccgctgatac cactttacag tattt
//
LOCUS       DATNDD010000355         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3877967, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000355 DATNDD010000000
VERSION     DATNDD010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3877967"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /gene="secY"
                     /locus_tag="VN647_08595"
     CDS             <1..1175
                     /gene="secY"
                     /locus_tag="VN647_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWG97126.1"
                     /translation="EFLQKQGGSLLGFLDIFSGGSLSRLTIFALGIMPYISASIILQL
                     LTVVIPHLTKLAKEGERGRKKIIQYTRFGTIGIALIQGFGIAIGLEQMNQGAFVLNAG
                     WGFRLMTVITLTAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPAAVAQTYNLYE
                     IGQLNAFLLIALALLMIGVVAAIVFLESGRRKIPVQYAKRVIGRRVYGGQSTHIPLKI
                     NTAGVIPPIFASSIIAFPATIAGFFETPWVKSIGAQLAPGSLLYTLMYVGLIIFFCFF
                     YTAVVLNPVDMADNMKKYGGFIPGIRPGQRTSDYIYNVLTKITFAGAVYLAIVCVIPE
                     FLIYKLNVPFYFGGTSLLIVIGVGLDTAQQIESHMLMRNYEGFLGKGMAPLRGRSG"
     gene            1180..1836
                     /locus_tag="VN647_08600"
     CDS             1180..1836
                     /locus_tag="VN647_08600"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWG97127.1"
                     /translation="MRLVFLGAPGVGKGTQADKAAVRYRIRKLSTGDLLREAVRNKTH
                     LGREAKEHMDQGRLVPDSVVIGLVREKLADPAYGNGFILDGFPRTVPQAEALAKVLAE
                     RSLHLDRVINFSVSREEIVKRLSGRRSCPKCQATYHVEFAPSKNGNLCERCGEALVQR
                     SDDQREAIETRLHVYDEQTAPLINFYEKQRLLSHLNGAEAVETVYHNLVKALAAPQTA
                     "
     gene            1833..2591
                     /gene="map"
                     /locus_tag="VN647_08605"
     CDS             1833..2591
                     /gene="map"
                     /locus_tag="VN647_08605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWG97128.1"
                     /translation="MIILKTPAEIAVMAEASRVVAEALAIVKKAVSPGISTEELDRIA
                     EEAIRIRGAVPAFKGYRNYPKTLCASVNEQVVHGIPSKRKLKEGDIIGLDLGAIVGGF
                     YGDSAVTVAVGRVPEETEKLIQVTEEALYLGIQQAVVGNRLTDISNAVQQHVESAGFS
                     VVTEFVGHGIGRQLHEEPQVPNYGKAGQGPRLQPGMVLAIEPMVNMGRSAVRVLEDRW
                     TAVTVDGSLSAHFEHTIAVQPSGAPRILSQLEKA"
     gene            2624..2842
                     /gene="infA"
                     /locus_tag="VN647_08610"
     CDS             2624..2842
                     /gene="infA"
                     /locus_tag="VN647_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWG97129.1"
                     /translation="MAKEDIIEVQGSVAETLPNAMFRVKLDNGHVILAHISGKMRMHF
                     IRILPGDKVTVEMSPYDLTRGRITYRFK"
     gene            2861..2974
                     /gene="rpmJ"
                     /locus_tag="VN647_08615"
     CDS             2861..2974
                     /gene="rpmJ"
                     /locus_tag="VN647_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWG97130.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRILCKNPRHKQRQG"
     gene            3091..3489
                     /gene="rpsM"
                     /locus_tag="VN647_08620"
     CDS             3091..3489
                     /gene="rpsM"
                     /locus_tag="VN647_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWG97131.1"
                     /translation="MARIAGIDLPRNKRTDIGLSYIYGIGRISAQKILNEAGIDGAVR
                     VKDLSEDKIVKLREIIERDFRVEGDLRKEVSLNIKRLIDTGTYRGLRHRKGLPVRGQR
                     TKTNARTRKGRRAGVSSKPRPTVTKRASST"
     gene            3517..>3641
                     /gene="rpsK"
                     /locus_tag="VN647_08625"
     CDS             3517..>3641
                     /gene="rpsK"
                     /locus_tag="VN647_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWG97132.1"
                     /translation="MSVKKGKKKERRIVQSGVAHVQASFNNTIVTITDMSGNTVV"
BASE COUNT          879 a          816 c         1080 g          866 t
ORIGIN      
        1 ccgagttttt gcagaagcag ggcggctctt tgctggggtt tctcgacatt ttctccggcg
       61 ggtcattatc ccgtctgacg atctttgcgc tgggtatcat gccgtacatc agcgcatcga
      121 tcattctcca attgttgacg gtcgtcatcc cgcatttgac caaattggcc aaagagggcg
      181 agcggggtcg aaagaaaatc attcagtata cccggttcgg aacgatcggt attgccttga
      241 ttcaagggtt tggtatagcg attggattag aacaaatgaa tcagggagcg ttcgtgctca
      301 atgccggatg gggattccgt ctcatgacgg tcatcacctt gacggctggc accggtttcc
      361 tgatgtggct cggtgaacag atcaccgagc gaggaatcgg caacggaatc tctttaatca
      421 tttttgccgg tattgtcgcc agactgcctg cggctgtggc ccagacttat aatctgtacg
      481 aaatcggcca gctcaatgcg ttcttattga ttgcattggc tctgttgatg atcggcgtcg
      541 tcgccgcgat tgtttttttg gaaagcgggc ggcggaaaat tccggtgcag tatgcaaagc
      601 gggtgatcgg gcgacgggtc tatggagggc agagtacgca cattccgttg aagatcaata
      661 cggctggggt cattcctccc atatttgcct cgtcgatcat cgcgtttcct gcgaccattg
      721 cggggttttt tgagacgcca tgggtgaaat cgatcggggc acagctggct ccgggatcgc
      781 tgctctatac gctgatgtat gtaggactca tcatcttttt ctgtttcttc tacaccgcgg
      841 tggttcttaa tcctgtcgat atggcggata acatgaaaaa gtacggaggt tttattcctg
      901 gtatcaggcc cggtcaacga acctctgact atatctacaa tgtcctcacg aagattacat
      961 ttgcgggggc tgtctatctg gccatcgtct gtgtgattcc tgaatttttg atctataaac
     1021 tgaatgttcc tttttatttt ggtggtactt ctctgctgat tgtcattggt gtgggcctgg
     1081 atacggcaca gcaaattgaa tcccatatgt tgatgcggaa ttacgaggga tttttaggca
     1141 agggcatggc tccgttgcgc gggagaagcg gctaggatca tgcggcttgt gtttctcggt
     1201 gctccgggtg ttgggaaagg cacacaggct gataaggccg ctgttcggta tcgcattcgt
     1261 aaactttcga ccggtgattt gctacgggag gcggttcgaa ataagaccca ccttggccgt
     1321 gaagctaaag agcatatgga tcaaggaagg ttggtcccgg attcagtcgt cataggacta
     1381 gtacgggaga agctggctga tcccgcatat ggcaatgggt ttattcttga tgggttccca
     1441 aggaccgttc ctcaggcgga agcgcttgcg aaggtgctcg ccgaacgaag ccttcacttg
     1501 gatcgagtga ttaatttcag cgtttctcga gaggaaattg tgaagcggct gagcgggcgt
     1561 cggagctgtc ccaagtgtca ggcgacctat catgtagagt ttgctccatc caagaacggc
     1621 aatctctgtg aacggtgcgg agaagcgctg gttcagcgaa gcgacgatca gcgcgaggcg
     1681 attgagacgc ggctccacgt gtacgatgag caaacagcgc cgttgatcaa cttttatgag
     1741 aagcagcggt tattatcgca tcttaacggt gccgaggcgg ttgaaacggt ataccacaac
     1801 ctcgtgaaag cgctcgcggc accccagacg gcatgatcat tctgaaaacg cccgccgaga
     1861 tcgcggtgat ggcagaggcg tcaagagtgg tggctgaagc gctcgcgatt gtgaagaaag
     1921 cggtcagtcc aggtatcagt acggaagagc tggaccgcat tgccgaggag gcgatacgga
     1981 tcaggggggc ggttccggcg ttcaaaggct accggaacta tccaaaaacc ctctgtgcat
     2041 ccgtgaacga acaggtcgtt cacggtattc cgtcgaagcg aaagctgaag gaaggggata
     2101 tcatcgggct ggatctcggt gctattgtgg gggggttcta tggagattca gcggtaacag
     2161 tggcggttgg tcgggtcccg gaggagacgg aaaaattgat tcaagtgacg gaagaggcgc
     2221 tctatcttgg aatccaacag gctgtagtcg gcaatcgttt gacagacatc tcgaatgctg
     2281 tgcagcaaca tgttgaatcc gcgggcttct cagttgtcac ggagtttgtg gggcatggga
     2341 ttggtcggca attgcatgaa gagcctcaag ttcctaacta tgggaaggcg ggtcaggggc
     2401 ctcgcttgca accaggtatg gtcctggcca ttgaaccaat ggtgaatatg ggtcggagtg
     2461 ccgttcgtgt tctggaggat cggtggacag cagtcacggt agatgggagc ctatctgctc
     2521 atttcgagca tacaatcgcc gtccaaccaa gcggagcgcc ccgcattttg agtcaattgg
     2581 agaaggccta gatttaatcc atcaggaaag aatacagaag gacgtggcaa aagaagacat
     2641 tattgaagtc cagggctcgg tagcggagac gcttcctaac gccatgtttc gggtcaagct
     2701 ggataatggc catgtcatat tggctcacat ctcggggaaa atgcggatgc attttatccg
     2761 tatccttccc ggcgataagg tcacggtgga aatgtcacca tacgatttga ccaggggccg
     2821 aattacctat cgcttcaagt aacgggagtg gcgaattgtt atgaaggtca aatcatcagt
     2881 gaagcccata tgtgccaaat gcaaggttgt gcgtcgtcgg ggggtcgtgc ggattctatg
     2941 caaaaaccca cgtcataaac agcgacaagg atgaagaaaa gtgccgagcg atgagtgctg
     3001 agttctgagc cggcattaca gacagtctca atgaggagac cttttattac ttagaactca
     3061 aggctcagca ctcagaactg gaggggatga atggcacgta ttgccggcat cgatttgcca
     3121 cggaataaac gaacggatat cggattgagc tacatctatg gaatcggacg catatcggct
     3181 cagaagattc tcaatgaagc ggggatcgat ggagcggttc gtgtcaagga cttgagcgaa
     3241 gataagatcg tcaagttgcg tgaaatcatt gagcgtgatt tccgggtcga aggagaccta
     3301 cgcaaagaag tgtcgctgaa tatcaagcgg ctgatcgata cggggaccta tcgaggattg
     3361 cgtcatcgca aaggtcttcc cgtccgtgga caacgaacca agaccaatgc ccggacgcgt
     3421 aaaggacggc gcgcaggcgt cagtagcaag ccgagaccca cggtcactaa aagggcctca
     3481 agcacctagc ctaccgaaac agccggagga tttcacatga gcgtcaagaa agggaaaaag
     3541 aaagaacgtc ggattgtcca aagtggagtg gcacatgtcc aggcgtcatt caacaatacg
     3601 attgtgacca tcactgacat gagcggaaac acagtggtat g
//
LOCUS       DATNDD010000356         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3014841, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000356 DATNDD010000000
VERSION     DATNDD010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014841"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VN647_08630"
     CDS             complement(<1..915)
                     /locus_tag="VN647_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWG97133.1"
                     /translation="MPLITKDLIAQHNLTGEEYQKIVDILGREPNLTELGMFSVMWSE
                     HCSYKSSRMHLKKLPTTGPRVVQGPGENAGAVDIGDGLCVVFKMESHNHPSFIEPYQG
                     AATGVGGILRDIFTMGARPIALLDSLRFGELHSPKNRHLMKGVVSGISGYGNCMGVPT
                     VGGEIVFNDIYSLNPLVNVFCLGLARQDKIFRGTAVGVGNPVIYFGSKTGRDGIHGAT
                     MASDSFDDQSEQKRPTVQVGDPFTEKLLLEACLELMERDLLVGIQDMGAAGLTSSSCE
                     MASRAGNGIELDLTVVPRRESGMTPYEIM"
     gene            complement(1085..1423)
                     /locus_tag="VN647_08635"
     CDS             complement(1085..1423)
                     /locus_tag="VN647_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97134.1"
                     /translation="MRRVGAVLAAVGLLTLGVGVNVWAAADDGSQVKITSPAEGAVVK
                     GGDVEIKYELTKGSKATHVHCFVDGEYQKGWKGMVKGMSPGTREIKLVAADKDHETLA
                     AESAIKVEVQ"
     gene            complement(1535..>2029)
                     /gene="purQ"
                     /locus_tag="VN647_08640"
     CDS             complement(1535..>2029)
                     /gene="purQ"
                     /locus_tag="VN647_08640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HWG97135.1"
                     /translation="GLVFGICNGFQILLEAGLLPGVMLRNRSLHFICRETYVKVENAA
                     TPFTNGCRPGQVLKIPIAHADGNYYIDPVTLAGLQANAQIILRYCTAEGKVTPEACPN
                     GSLDNIAGIRNAAGNVLGMMPHPERCAETVLGNEDGRTIFQSMIASLERKKLAAVVSA
                     PTTI"
BASE COUNT          453 a          631 c          492 g          453 t
ORIGIN      
        1 catgatctca tagggcgtca taccagactc gcgccgtggt accaccgtga gatccaactc
       61 gatcccgttg ccggcgcggg aggccatttc gcaggaagaa ctcgtcaggc ctgcagcacc
      121 catatcttga atcccgacga gcagatcacg ttccatcagt tcgaggcaag cttccagcag
      181 caatttttcc gtaaagggat caccaacctg gacggtcggc cgcttctgct ccgattgatc
      241 gtcgaacgaa tcggacgcca tcgtcgcccc gtgaatccca tcacgtcctg ttttggagcc
      301 gaaatagatt acaggattcc cgacgccgac agccgtgcca cggaatatct tgtcctggcg
      361 ggcaagaccg agacaaaaca cattgaccaa cggattgagg gagtagatgt cgttgaagac
      421 gatctcgcct cccacagtcg ggactcccat acagttgcca tagccggata tcccggagac
      481 gacccctttc atgagatggc gattcttggg cgagtgcaat tctccaaacc gcagcgaatc
      541 gagcagcgcg atcggtcttg cccccatcgt aaagatgtcg cgcaaaattc cgccgactcc
      601 cgtggcagcg ccctgatagg gctcgataaa cgacggatgg ttgtgcgact ccatcttgaa
      661 gaccacacac agcccgtcac cgatatcgac tgctcccgca ttttctcccg gcccttgcac
      721 gacacgaggc ccggtcgtcg gcagcttttt caggtgcata cgagaactct tgtaggagca
      781 gtgctcggac cacatcacgg aaaacatgcc gagctccgtc aggttgggct cacgcccgag
      841 aatgtcgacg attttttggt attcctcgcc cgtcagattg tgctgagcga tgaggtcttt
      901 ggtgatgagc ggcatgccgg cctgcattac gacctagcgg tcacagagac aaaaccgctt
      961 tggagcgttc cgaacaccag ggtatgcgtg acaccgttgc aagcattgct cgaggtgacc
     1021 ggcgaggcgc acattcggcg cctcgccgag tcgttctgtg tgatcaacga accccgatcc
     1081 tgttctactg cacttccact ttaatggctg attcagcggc taaggtttca tgatccttat
     1141 ctgccgcaac gagcttgatc tcacgagtcc cgggtgacat tcccttgacc attcctttcc
     1201 atcccttctg atattctcca tccacgaaac aatggacgtg cgtcgccttg ctaccctttg
     1261 tcaactcata cttgatttca acatcgccac cctttacgac ggccccctct gccgggctgg
     1321 tgatcttcac ctgactgccg tcatctgccg cagcccaaac attgacacca acacccaagg
     1381 tcaacagacc gactgccgcc aacactgctc ctacacgtct catcgcaacc ctcctcatga
     1441 tgtatgaaaa aagttaaggc tgatacatac cgactgactc cacactttac cgcgaccatc
     1501 taccatcttc aatcgtatgg agagcattct tcggttaaat cgtcgttgga gcgctcacta
     1561 ctgctgccaa tttctttcgc tccaaagacg cgatcatcga ttgaaagata gttcgcccat
     1621 cttcatttcc gagtaccgtc tccgcgcagc gttccggatg cggcatcatg cccaggacat
     1681 tgcctgcggc attgcggata cccgcaatat tatcgagtga cccattcgga caagcctccg
     1741 gcgtcacctt cccttccgcc gtgcaataac ggaggatgat ctgagcattg gcttgaaggc
     1801 cggctaatgt cacgggatcg atgtagtaat tcccgtccgc gtgagcgatc ggaattttga
     1861 gcacctgtcc gggcctgcag ccgttcgtaa acggcgtcgc agcgttctcc accttcacgt
     1921 aggtctcgcg gcagataaag tgcaacgacc ggtttcgcaa catgacgccg ggcaacaaac
     1981 ccgcttccag cagaatctgg aatccattgc agatcccaaa caccaatcc
//
LOCUS       DATNDD010000357         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2181375, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000357 DATNDD010000000
VERSION     DATNDD010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181375"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..789
                     /locus_tag="VN647_08645"
     CDS             208..789
                     /locus_tag="VN647_08645"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HWG97136.1"
                     /translation="MIEKTTGRILGKLLVILLAGVSVNVAQAGEPEGIPPELVAEYIY
                     AVIQADRSIYSTHVVEQLQDRKVAVAAEDWKERGALPLPAQFLQMAGQEIQGLGLGLH
                     VRLGSLGPIYTKNGPVDQFERAGLEAVAKNPRKPYTGIITEGDRRYFKAIFADRAVSM
                     TCVICHNSHPLSAKRDYKLHDVMGGIIISFPVR"
     gene            complement(805..1836)
                     /locus_tag="VN647_08650"
     CDS             complement(805..1836)
                     /locus_tag="VN647_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97137.1"
                     /translation="MVPEFYRKTEATQRTIARLIAFLGFVLFGGMATTPALSENPAPS
                     VHWGSMAFPDQYSTFTGGLTLDRFTPTDGLGNKYDSTVGNTLGFNLMTFSWTQHWGGN
                     WRNWSTNLTAGISPTGDEPSQYFQNEVVHQLRQLPTVPTVDPRRETDAMIDGSITRWF
                     SLWCGPKVIFAGGGFSVGTNYQQGFLRAGIRRLQIIPTLYSASWGDVSARASVLGRIS
                     YQDNGSTIHDVRQTAGLVQPSIAFGQYHTTANGGTVPTWEIEFALMWDSGIFVNVEGV
                     SQKQFAWSIAGSFGPVRFETWNDSMGNIAERDFGPTYGATLTLDVRRVWNILQCLRST
                     PSPGLPASA"
     gene            2147..>2789
                     /locus_tag="VN647_08655"
     CDS             2147..>2789
                     /locus_tag="VN647_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWG97138.1"
                     /translation="MFKKILVANRGEIAMRIIRACRELNIATAAIYSEADSTGIYVKK
                     ADESYMVGPGPVKGFLDSAQIVDLAKRIGADAVHPGYGFLSENAEFAELCQASGITFI
                     GPSTRAITLMGSKVKAREIAESSGVPIVPGTDGAITSIKDALAFVKQSGYPVMIKASA
                     GGGGRGLRVVRSDDELRENMEVAAREAQASFGDGSVFIEKYIERPHHIEFQILG"
BASE COUNT          633 a          762 c          770 g          624 t
ORIGIN      
        1 gtcatgctcc gcttcagtct ccctgaagcg agctttgatc ggtttcactt ctggtgcaat
       61 ctccatggaa aattgcttac cgacgtcatg cgcggggaga tctttatcct agatgcgaaa
      121 caatcatcca agtaactgga cctcggaaat gttgaggacg tgctcttgtg agcaggttcc
      181 gccctgagag ttaaaggaga ggttgtcatg attgagaaaa caaccggacg gatccttggc
      241 aaactcttgg tcattctgct cgccggtgtc tccgtgaatg ttgcgcaggc cggagagccg
      301 gagggaatcc ctccggagct ggtggcggaa tatatttatg cagtcattca ggccgatcga
      361 agcatttatt ccactcatgt cgtcgaacag ctgcaggatc gcaaggtcgc tgtggcggca
      421 gaagattgga aagagcgggg ggcattgcct cttccagccc aattccttca gatggcgggg
      481 caagagattc aggggctagg cctcggtctc catgttcgcc tgggcagctt aggcccaatt
      541 tacaccaaga acggaccggt ggatcagttt gaacgagcgg ggttggaagc ggtagccaag
      601 aatcccagga agccctatac gggaatcatt acagagggtg atagacgtta tttcaaggca
      661 atttttgcag accgggccgt ctcgatgacg tgcgtgatat gccataacag tcatcctctg
      721 agtgccaagc gggactacaa actccatgat gtgatgggag ggattattat ttcttttcca
      781 gtccgttaga gcaaacgagg aatcttacgc cgaggccggc aaaccagggg agggggtcga
      841 tcggaggcat tggagaatgt tccagactcg ccggacatcg agggtcagcg tcgcgccata
      901 ggtcggccca aagtcacgct ctgcgatgtt tcccatgcta tcgttccacg tttcgaaccg
      961 gaccggtccg aatgagccgg caatcgacca ggcgaattgc ttctggctca caccttccac
     1021 attcacaaaa attcccgaat cccacatgag cgcaaactcg atttcccaag tgggaacggt
     1081 tccgccatta gcggtggtat ggtactggcc gaaagcaatc gagggttgca caagccctgc
     1141 cgtctgtctc acatcgtgaa tggtggatcc gttatcctga tagctgatcc gtccgagaac
     1201 agaggcccgt gcactcacgt ccccccaact agcggaatag agcgtcggta taatctgtaa
     1261 ccgcctgata ccggcgcgga gaaacccctg ttgatagttc gtcccgacgg aaaacccgcc
     1321 ccctgcgaag atgaccttcg gcccacacca caaggagaac caccgagtta tagacccatc
     1381 gatcatcgca tcggtttccc gacgagggtc gaccgtcgga acggtcggca gttggcgaag
     1441 ctgatgcacg acctcatttt gaaaatattg actgggttcg tctcccgttg ggctgatccc
     1501 ggctgtaagg ttcgtgctcc agttccgcca attcccgccc caatgttggg tccagctgaa
     1561 cgtcatcaga ttgaagccga gcgtattccc taccgtggaa tcatacttgt tccccagccc
     1621 atccgtcggg gtgaaccgat cgagcgtcag ccctcctgta aacgtcgaat attggtcggg
     1681 aaaggccata ctcccccaat ggactgaggg agcggggttt tcgctcagtg ccggggttgt
     1741 tgccatgccg ccgaacagga caaatccgag gaaggcaatc agtcttgcaa ttgtcctctg
     1801 agtggcttct gtcttgcgat agaattccgg taccatccta tgcccgttcc cagtctcgct
     1861 gaacggttgc ctcattggaa gaggaatggt tctgaccatc aggaagctct cgctgcgacg
     1921 aatcattgcc gtaccatcat gagaaaccta gggcattcta tagaactcat gcgcgtgtga
     1981 cgacccaatt gtttaatgaa agacgaacaa gaacaacact tcgttttgat gagcgattcc
     2041 cacctctccc tcgcgcttct ccctcgacag acctcttgat cctatgctag tttacgcgtt
     2101 cgtttttcag attcatttct ttccgcacga gctgacgggc tcagacatgt tcaagaagat
     2161 cttagtcgcc aatcgcggtg agattgccat gcggatcatc cgtgcctgcc gcgaattgaa
     2221 catcgccacc gctgccatct attccgaagc ggattcgacc gggatctacg tgaaaaaagc
     2281 ggatgaatcc tacatggtcg ggcccggccc ggtgaagggt ttcttggata gcgcacagat
     2341 cgtagacttg gcgaaacgga tcggcgccga tgccgtccat cccgggtacg gatttctttc
     2401 tgaaaatgcg gagttcgccg agctctgcca ggcatccggc attacgttca ttggtccttc
     2461 tacccgggcc attaccctga tgggcagcaa ggtcaaagcg cgcgagatcg ccgaatcttc
     2521 cggtgttccg atcgtgccgg gcacggatgg agccattacc agtataaaag acgccctggc
     2581 cttcgtaaaa caatccggtt acccggtcat gatcaaggcg agcgccggcg gcgggggccg
     2641 cgggcttcgc gtggtccgat cagacgatga gctgcgagag aacatggaag tcgccgcgcg
     2701 agaagcccag gcgtccttcg gcgacggcag cgtcttcatc gaaaaatata tcgagcggcc
     2761 acaccatatt gaattccaaa tactgggag
//
LOCUS       DATNDD010000358         4019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3316308, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000358 DATNDD010000000
VERSION     DATNDD010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3316308"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VN647_08660"
     CDS             complement(<1..1453)
                     /locus_tag="VN647_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HWG97139.1"
                     /translation="MSTEKPIPVTTPESVSTDPVDRVIHYHVRTKHHFNRYARSLGFL
                     DWASQPDPFRRFEGAQLIPLPLLKADEEPLSPSYQAIYEREAVSAQPVNMRMLSRFFE
                     FALALSAWKKAGESEWALRSNPSSGNLHPTEGYVVLPQLKGFDLKPGLYHYAPKEHGL
                     ELRAGFRADLLECLLGSFAQDAFLLGLTSIHWREAWKYGERAFRYCNHDVGHAIGAVR
                     IAAATLGWNMTLLDGVDQDTVAMLLGTDRKEDFGEAESEHPDCLAVVWPNEAVKSKGL
                     EVRGREEVTDGKKGMPLFLDTSIVKKLAGETWHGKANRLSLEHGVHWDIIDEAAKASW
                     KLETDERSVSLPSPQVTPHPLPLTPHAPSSGHIIRRRRSAVAFDGKTSISAATFFHMM
                     QRVMPWAEHPQLQRPMPWDAWPYDPVIHLLIFVHRVEGLTPGLYVLVRDNRKLSVVQH
                     TMNPEMTWTIASGCPEGLPLYWLLEGDARKMAAQ"
     gene            1589..2011
                     /locus_tag="VN647_08665"
     CDS             1589..2011
                     /locus_tag="VN647_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97140.1"
                     /translation="MRFDPALAAQTAFKESESELGSDRDTAVELEETFSSNAGVTARE
                     AYEGLLAIAQHYPNAHSFHAFCIYITWQQVTEETIAHHFETGLKLSEAYLASHEGKNP
                     RDIEHITELHGSFRAGLGLEEQDDLQVEYKRDTPKGGD"
     gene            2117..2881
                     /locus_tag="VN647_08670"
     CDS             2117..2881
                     /locus_tag="VN647_08670"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG97141.1"
                     /translation="MSDNHKHRARIFLHRGDLVQARAAWEAAVADDRTAGSQQELSDS
                     LGNLGNTCALMNDFPRAEQCYREVLQIQRTERNLTAVAHTLVNLGNLHIASDHPEKAR
                     PYYLEAMDLLRQVQDNRGLGILYNNLALQEARDGQWDQAVASFTQALDHHRMVGNEEG
                     LAVTYSQLGKCYFDQGDLTRAERCLNNASEHYIKLGNEPAEAAVLRLLAKVYETRQDT
                     VSTLRCLARVVALDERYALPELQADSAHLSGLGPSR"
     gene            3027..3629
                     /locus_tag="VN647_08675"
     CDS             3027..3629
                     /locus_tag="VN647_08675"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HWG97142.1"
                     /translation="MASLARRFSILAVGTMMTASFVQAAEISPTITVGTQEVGLTAGY
                     MFSHRLTHLHTTKQHGPALMPSWMMTITDPIGDSWYRGQVSLGAEMVYLEFREPFLTH
                     GVGFTPRIKYTFVALGRIRPYAEFAGGPFWTDLGGRIHEEATQFNFVLTGGVGVSWFL
                     TPQLAFNAGYRFHHISNAGTAFPNLGLNASLPFAGFSFYF"
     gene            3643..>4019
                     /locus_tag="VN647_08680"
     CDS             3643..>4019
                     /locus_tag="VN647_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97143.1"
                     /translation="MTMNMLRWSVLSLAGFVLCGITGCTRWIDVKPPSADPQETVTHS
                     LPNDERVPLVTNSFHLSRNGSSQYPSVDLERRILSSIQETSLFSTLMPLGEKPDSLGD
                     KVVSARITVDETIDPHSWLSAFKG"
BASE COUNT          915 a         1158 c         1039 g          907 t
ORIGIN      
        1 cctgagcggc catttttctg gcatcgcctt ccaataacca atacagaggc aggccctcgg
       61 gacagccgga cgcgatggtc caggtcattt ccggattcat ggtgtgctga acgacagaca
      121 actttctgtt atctcgtaca agaacataca gccccggtgt aagcccctct acacgatgaa
      181 caaatatcaa cagatgaatg accggatcat agggccaggc atcccacggc atcggccgtt
      241 gcaactgcgg atgttcagcc cacggcatga cccgttgcat catgtgaaaa aacgtggctg
      301 cggaaattga tgtcttgccg tcaaatgcga cggcgcttcg acgccggcga atgatgtggc
      361 cggacgaagg tgcgtgaggc gtgaggggta aggggtgagg agttacttgc ggagacggaa
      421 gcgaaactga acgttcatcg gtttcaagct tccaagatgc ttttgccgcc tcatcaatga
      481 tgtcccaatg gacgccatgc tcgagactca accgattggc ctttccatgc catgtttcgc
      541 cggccagttt tttcacgatc gacgtatcga gaaacaacgg catccccttc tttccgtccg
      601 ttacctcttc acggcccctt acctctaatc ccttgctctt tactgcttca ttaggccaga
      661 ccacggccag gcagtcagga tgctccgatt ccgcctctcc aaagtcttcc ttgcgatctg
      721 tcccgagtag catcgccacc gtgtcttgat ccacaccgtc cagcagcgtc atattccagc
      781 ccagcgtggc agcagcgatt cgtactgccc cgatcgcatg tccgacatcg tgattgcaat
      841 agcggaatgc tcgctcgcca tacttccagg cttcgcgcca atgaatcgac gtgagtccca
      901 agaggaaggc atcttgagca aaagacccca gtagacattc aagtaagtca gcacgaaacc
      961 ctgcccgtaa ctcgagtcca tgttctttcg gtgcatagtg atagagcccc ggcttcagat
     1021 cgaacccctt cagctgaggc aagacgacat agccttccgt gggatgcaaa ttgccggacg
     1081 atggattgct tcgtagcgcc cattcggatt cacctgcctt cttccaagcc gacagcgcca
     1141 gggcgaattc gaaaaatcga gagagcattc gcatgttcac gggctgagca gagacggctt
     1201 ctcgttcata aatcgcttga taggaaggcg acaagggctc ttcatcagcc ttgagtaatg
     1261 gaagtgggat aagctgagct ccctcaaacc gcctgaacgg atcaggctgg ctcgcccaat
     1321 caagaaatcc caacgaacgg gcgtaacggt tgaaatggtg tttggtcctg acatgatagt
     1381 ggatgacgcg atcgactgga tctgtcgaaa ctgactcagg cgttgtaacg ggaattggtt
     1441 tttcagtgct cattctaaga aataagtgca gtctacagag ctccacaatc taaatctatg
     1501 gtctgagcaa gacacattga cctcctttcc tttaccccag tatcctctag ggtacttgta
     1561 atacttgctg tatgagatac gaggttctat gcggtttgat ccagccttgg ccgcacaaac
     1621 agcgttcaaa gagtctgagt ccgaactcgg ttccgaccgg gacacagctg ttgagctgga
     1681 ggaaacgttt tcctccaatg ccggtgtgac agcgcgcgaa gcctatgaag gtctgttagc
     1741 catcgcgcag cactatccca acgctcattc gttccatgcc ttttgtattt acatcacttg
     1801 gcagcaggtc acggaggaga cgatcgcgca tcatttcgaa acaggcttga aattgtctga
     1861 ggcctatctg gcctctcacg aagggaagaa tcctcgggac atcgagcaca tcacggaatt
     1921 gcatggctcc ttccgagctg gtctagggct ggaggaacaa gacgatctgc aggttgaata
     1981 taagcgagac acgcccaaag gcggagactg acgaaatccg tgaggggtaa ggcgcaaggg
     2041 gtgaggcgaa cgagacagac agaacccttg ggttatttcc ctccccaccc ttcacgcttc
     2101 acgcctcacg gaaaaaatgt cggataacca taaacatcgc gcacggattt ttctccatcg
     2161 cggcgatctg gtccaggctc gcgctgcatg ggaagcggct gtggccgatg atcggactgc
     2221 cggcagtcag caagagctgt cggattctct tgggaacctc ggcaatacct gtgcgctcat
     2281 gaacgatttc ccgcgtgcgg aacagtgcta tcgagaggtt ctccagattc aacggaccga
     2341 gcgcaatctc accgccgtcg ctcacacctt ggtcaacctc ggcaacctgc atatcgcctc
     2401 cgaccatccg gagaaagccc gtccctacta tctcgaggcc atggatctac tgcgccaggt
     2461 acaagacaat cgtggactcg gcattctcta caacaacctt gcgctccaag aggcccgtga
     2521 cggtcaatgg gatcaggccg tcgcgtcatt cacacaagcg ctcgatcatc atcggatggt
     2581 cggcaacgaa gaaggtctcg ccgtcaccta tagccaactc ggcaaatgct atttcgatca
     2641 gggagacctc accagagccg agcgttgttt gaacaacgcc tcggagcact acatcaaact
     2701 cggcaacgaa ccggctgagg ccgcggtact ccgtcttctc gcaaaggttt acgagactcg
     2761 ccaggatact gtctccactc tgcgatgcct cgcgcgtgtg gtggcgctgg acgaacgcta
     2821 tgcgctgcct gaacttcaag ccgactccgc ccacctctcc gggctcggtc catctcgata
     2881 actactttct ctctagccac gccaaatccg accgccgact tctggacaga atccgatcct
     2941 ctgctacctt tggaacagca catctggacg cctgttgatc aagtttcacc tctcacaagc
     3001 cgacaagata ttgacgtgga gatcgtatgg cttcgctcgc tcgacgattc agtattctgg
     3061 cagtcggaac gatgatgacg gcatcctttg ttcaagctgc agagatttcc cctacgatca
     3121 cggtcggcac ccaagaagtc ggacttaccg ccggctatat gttctcccat cgactgaccc
     3181 accttcatac gaccaaacag cacggacccg cgttgatgcc gtcgtggatg atgacgatca
     3241 ctgaccccat cggcgacagc tggtatcgag ggcaggtctc gcttggcgcg gaaatggttt
     3301 acctcgagtt ccgagagcct ttcttgacgc atggcgtcgg attcacgccg aggatcaagt
     3361 acacattcgt cgccttggga cgtatccgcc cgtatgcgga atttgcgggc gggccgtttt
     3421 ggaccgatct tggcggtcgc attcatgaag aagccactca gttcaacttc gtgctgacag
     3481 gaggagtggg cgtgtcctgg tttctcacgc cgcaactcgc cttcaatgcg ggctatcgtt
     3541 ttcaccacat ttccaatgcc ggcaccgcct tcccgaatct tggactgaat gcgagtttgc
     3601 cttttgcggg gttttcattc tatttctgag gagacgacga tcatgacaat gaacatgttg
     3661 cgatggtcgg tcctgagtct tgccggattc gtgctgtgcg gcataactgg atgtactaga
     3721 tggatcgacg tcaaaccgcc ctcagccgat cctcaagaga ccgtgacgca ctcgctcccc
     3781 aatgatgaac gggtcccgct ggtgacgaat tcgtttcacc tctcacgaaa cgggtcgtcg
     3841 caatatcctt ccgttgatct cgagcggcgg atcttaagca gcatccaaga aaccagcctg
     3901 ttttcgacat tgatgccgtt gggagaaaag cccgattcgc tcggagataa ggtcgtctcc
     3961 gcgcgcatca ccgtcgacga aaccatcgat ccccattcct ggctctcggc gttcaaagg
//
LOCUS       DATNDD010000359         3846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_712217, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000359 DATNDD010000000
VERSION     DATNDD010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712217"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VN647_08685"
     CDS             complement(<1..302)
                     /locus_tag="VN647_08685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWG97144.1"
                     /translation="MIPLHDDNPTQRTPIVTMILIGICIVVFFHQVNLPREAAELFAF
                     QYGAIPAIVFGQASLPEETIVIPTLLTLVTSMFLHGSWMHLLGNMLYLWIFGNNIE"
     gene            complement(315..1187)
                     /locus_tag="VN647_08690"
     CDS             complement(315..1187)
                     /locus_tag="VN647_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HWG97145.1"
                     /translation="MGNISSTPPFHGFPDIVLYHAECSDGFGAAWALWKKFPSANFLP
                     VKHGHPPPPDLKDRRVVIVDFSYARPTLEAMASETKELLVLDHHITAERTLDGFSNAY
                     FDQTKSGAVLSWEWAHGTAAPWLLQYIQDKDLWTWALPESREINAALASYPFDFNVWD
                     SFTQSTLKQEGRAILRYEHELVGKLAAQVSMVEFQGIVVPAVQSSILTSQIGERLSPH
                     HPFCLIWHDRDGRRYFSMRSRSDGTDVGRIAASFGGGGHTHAAGFSVPLEIDGTLPDN
                     PRLPRAVADRRRTP"
     gene            complement(1269..2324)
                     /locus_tag="VN647_08695"
     CDS             complement(1269..2324)
                     /locus_tag="VN647_08695"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HWG97146.1"
                     /translation="MNTTGRSPQIPPRPALSRDEILHQISALLDSREDDLHAALMTEL
                     LTGLLKLHDAHLDLLDVKIVNRAVKELRHAFSVFRGYRNRQKVSIFGSARTLSDDPNY
                     QLAYQFAQAIVREKFMVITGGADGIMRAAQEGAGRENSFGVNIMLPFEQGPNSTIADD
                     PKLITFKYFFTRKLMFQKEANAIALFPGGFGTHDEGFEILTLAQTGKSDPQPIICLQA
                     PGCDYWDDWSAFVTRQLLNRKLINEEDMSLFAIVNSAEAAVSQILKFYRRYHSIRFVG
                     RELAMRLKQTITPEQLERLRDRFADLLSDGTFELRGPLAEELDEPTLRDLPRLVFNFN
                     RRSTGRLRQLINHLNEL"
     gene            complement(2408..3184)
                     /locus_tag="VN647_08700"
     CDS             complement(2408..3184)
                     /locus_tag="VN647_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG97147.1"
                     /translation="MADKDDQQPKASDHQIILVAHMMTPGVVQIPGDVSVTEAASLLE
                     RERMPCLLVKDTEARFGLMTPTDIVKKVVAQGLEPDHIEVRTIMTRPVQFIEYDRSMD
                     EASALMMSTGTPILIVTKQDQPVGVLTARDLILSPKRCATNISATISVIEGEGVGAEH
                     QVTISQLSHAGASVESPALLLPGTKVILSFCLSEMMSPLTIRGAVLTNAGVEPVSGPT
                     GSFMAASRSVEIQFVALSPADQSRIKAWVLTNLPKSFDPA"
     gene            3350..>3846
                     /locus_tag="VN647_08705"
     CDS             3350..>3846
                     /locus_tag="VN647_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97148.1"
                     /translation="MMALNASYHREFIHRLRTIPAHGLGLSVDIHAPDLASLRRSLQE
                     RQVPPAYLEVFRTTPMALASTRKEAGNGLLTYHGEGLWITQPGMVDSTAFKQEIADAA
                     EQLMILQSAWLNHECATKYLAGYCYGTYLPPLYTPLSAKVVADNTRLIQHLLDQQCRL
                     ANGSTP"
BASE COUNT          899 a          964 c         1107 g          876 t
ORIGIN      
        1 tcttctatgt tgttgccgaa aatccagagg taaagcatgt tcccgagtag atgcatccag
       61 cttccgtgga gaaacatgct ggtcaccagg gtaaggagcg ttggaatcac aatggtttct
      121 tcaggaagcg aagcctgacc gaaaacaatg gcagggatgg caccatattg aaatgcgaag
      181 agctcggctg cctcccgagg aagatttact tgatggaaaa acacaacgat acagatgccg
      241 atgaggatca tcgtaacgat gggagtccgt tgagtgggat tatcgtcgtg gagcggaatc
      301 acggtggtcc gacattatgg agttcggcgg cgatcggcga ccgcacgagg caatctggga
      361 ttgtctggca gtgttccatc gatctcgagt ggaacggaaa atccagcagc atgagtatgg
      421 cctccgcctc cgaatgaggc ggcgattctc ccaacatccg taccatcgga tcgggaacgc
      481 atgctgaaat agcggcggcc atcacggtca tgccagatca agcaaaaggg atgatggggc
      541 gacagacgct cgccgatttg gctggtcaag attgagcttt gcacagcagg aacgacgata
      601 ccctggaatt ccaccatcga gacctgcgct gcgagctttc caacgagctc atgttcatac
      661 cgcaagatgg ctcgaccctc ttgcttgagc gtcgactgag taaaactatc ccatacattg
      721 aagtcaaacg ggtaggaggc caatgccgcg ttgatttcgc gactctccgg taacgcccac
      781 gtccataaat ccttgtcctg aatatactga agtaaccatg gagccgccgt accgtgagcc
      841 cattcccagc tcaagacggc accggacttt gtttgatcga agtatgcatt tgaaaagccg
      901 tccaaggtcc gctcggcggt gatatggtga tccagaacaa ggagttcctt ggtctcggag
      961 gccatggctt ccagggtcgg ccgggcatag ctgaagtcga cgatcacgac acggcgatcc
     1021 ttgaggtcgg gagggggcgg gtggccgtgt ttgactggca aaaagttggc actcggaaac
     1081 ttcttccaga gagcccaggc cgctccgaac ccatcggaac attcggcatg gtacagaaca
     1141 atgtcaggaa aaccatggaa cggaggtgtg gaggatatgt tacccataga gtcgggagag
     1201 aatagcatgc ccaggtcagg actaaaagac gtctgtggtg tccatgccta ctgttgatta
     1261 agaatgattc acaactcatt caagtgattg atcagttggc gaaggcgtcc ggtgctgcgc
     1321 cggttaaaat tgaacacaag tcgcgggaga tcgcgcaggg tcggctcgtc caattcttcc
     1381 gcgagaggac cacgtagttc gaacgtgccg tctgatagaa gatcggcgaa tcggtctcgg
     1441 agtcgctcaa gttgctcggg cgtaatggtt tgtttgagcc gcatcgcgag ttctcgtccc
     1501 acaaagcgta tggaatgata gcgtcggtag aatttgagga tctggctcac ggccgcttct
     1561 gcggaattga cgatcgcgaa gagactcatg tcttcttcgt tgatcagctt ccgattcagc
     1621 agttgcctcg tcacaaaggc cgaccaatcg tcccaatagt cgcagccggg ggcctgaagg
     1681 cagatgatcg gttgcggatc actcttgccg gtctgtgcca gtgtgaggat ttcaaaacct
     1741 tcgtcgtgtg tgccgaatcc gccggggaag agcgcgatgg cgttcgcttc cttttgaaac
     1801 atcaacttgc gggtaaaaaa gtatttgaag gtaatgagct tcgggtcatc ggcgatcgta
     1861 gagttaggac cttgttcgaa cggaagcatg atattgacgc cgaagctgtt ctcgcgtccg
     1921 gcaccctctt gagcagcacg catgatccca tcggctcctc cggtgatgac catgaacttt
     1981 tcccgaacaa tggcttgggc aaactggtag gctagttgat aattgggatc atcggagaga
     2041 gttcgtgccg agccgaagat gctgaccttc tggcggttgc gatagcctcg gaagacgctg
     2101 aatgcatgac gcagttcttt gacagcacga ttcacgattt tcacatccag gaggtctaag
     2161 tgcgcgtcgt ggagcttcag gagtccggtc aagagttctg tcatgagagc ggcatgcaga
     2221 tcgtcctccc ggctgtcgag aagggcactg atttgatgca ggatctcatc tcgtgataaa
     2281 gccgggcggg gaggaatctg cggagatctg ccggttgtgt tcatgattgt cctcttacgg
     2341 tgagtgagac gaaaattgta ccaatggatc ggacattggt caaagatttg tcgggatctg
     2401 atgcgatcta agcgggatcg aaagacttag ggagattggt cagcacccag gccttgattc
     2461 tggactgatc tgccggagag agagccacga attgtatttc tacactccga gatgccgcca
     2521 tgaaggaacc ggtggggccg gataccggct caactccggc gttagtcaag acggcccctc
     2581 gaatagtcaa gggactcatc atctcagaga ggcaaaagct caatatgact ttggtccccg
     2641 gcagcaacag tgcgggagac tccaccgagg cgccggcatg gctcagttgt gagatggtaa
     2701 cctgatgctc agctcccacg ccttctccct caatgacact gatggtggca gagatgttcg
     2761 tcgcgcatcg ttttggagaa aggatcaaat ctctcgcggt gaggacaccg accggttgat
     2821 cttgtttcgt cacgatgagg atcggtgtcc cggtggacat cataagtgcc gaggcctcat
     2881 ccatcgatcg atcgtactcg atgaattgga ccggtcgtgt catgatcgtc cggacttcga
     2941 tgtgatccgg ttcgagaccc tgcgcgacaa cctttttgac gatgtcggtc ggcgtcatga
     3001 gtccgaaccg agcctctgta tctttgacga ggaggcaggg cattcgctcg cgttccaaga
     3061 gcgatgccgc ctcggtgacc gatacatcgc ctggaatctg aactacgccg ggtgtcatca
     3121 tgtgtgcaac cagtatgatc tggtgatccg aggctttggg ctgttgatcg tccttgtccg
     3181 ccattcctca gcgaggtcct ctttcctcac gaatctgccc ccctcctggc tgcaatgtat
     3241 gcaaagtata gcagaaggta tgtggagacc atgagaggtt gaaagccttg tcattcaatg
     3301 gtttgacgac atacactagg gctgcatttt gcccccaagc aagagcgaaa tgatggcatt
     3361 gaatgcgtct taccatagag agttcattca tcggctcagg acaattccgg cgcatgggtt
     3421 ggggctttcc gtcgatattc atgctccgga cctcgctagc ttgcggcgaa gtctacaaga
     3481 acgtcaagtg ccgccggcct accttgaagt gttccgtacg acacccatgg ctttggcatc
     3541 caccagaaaa gaggcaggca acggcctgct gacatatcat ggcgaagggt tatggatcac
     3601 tcagcccggg atggtcgatt ctacggcgtt taaacaggaa atcgccgatg ctgccgaaca
     3661 actgatgatc ttgcaaagtg cctggctgaa tcatgagtgt gcgacaaaat atctcgccgg
     3721 ctattgttac gggacctatc ttccccccct ctacacgccg ttaagcgcga aggtggtcgc
     3781 tgataataca cgattgatcc aacatctgct cgatcaacag tgccggttgg ccaatggaag
     3841 cacgcc
//
LOCUS       DATNDD010000360         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2776622, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000360 DATNDD010000000
VERSION     DATNDD010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776622"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1773
                     /locus_tag="VN647_08710"
     CDS             265..1773
                     /locus_tag="VN647_08710"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWG97149.1"
                     /translation="MANWSQQATKMLIGSGLIVWALSGSGCVSPASAAGVPPAWAQGF
                     SDIVKRTTPAVVNIAVTGGGEGSRRRGGVPPSPFGTPPPGDEPGGGELPTPPPIPHGP
                     PMPHGRPDQSAGSGVVLDSNGYIVTNNHVVEGATQITVTLSDRREFSAKVVGTDPKTD
                     LAVIKIEAKDLPSLKWAEYDKLQVGDLVLAVGSPFGLSSTVTLGIISALGRGNVGIAD
                     YEDFIQTDAAINPGNSGGALVNMNGDLIGINTAIFSRTGGSEGIGFAIPSSIALDIVE
                     SLQRTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSHAAGVKRGDV
                     VVAFNGKDVQSVSQLRNLVARTVVGKDAQVKVLREGKEQLIVVKVAERPSDEMLAKKE
                     PAPPKEPGETMKLPDNVLASLRVQTLDNALMSQLNISAKTAGVVITSVDSGGPAEAAG
                     LQRGDVIQEVNHESIKSLSDYQKAAEKIKKDELAVLLVNRQGNSLFVAINPR"
     gene            1864..>2738
                     /gene="tatC"
                     /locus_tag="VN647_08715"
     CDS             1864..>2738
                     /gene="tatC"
                     /locus_tag="VN647_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWG97150.1"
                     /translation="MLNKLNRWLQDSVFKPLEDKKMPVMEHLVEFQVRLTRAVITLAV
                     IFMGTFFYADALVKWLRVPLQNMFVPSKLTWEPTDLPTVPFVFLAPAEALWQNVKVAG
                     LFAIVLAMPYLLFEIWRFVVPGLHVQERRFVGPFVCVSALAFYAGVGFSFFFVLPFAL
                     NFLISYGVNAGFVPQISIAQYVGFALWFLTVFGLIFEVPLTLTLMAKLGWVDAPFLIQ
                     YWKWALLGSFVIAAILTPTPDPFNQTLMAGPMFLLYWVGIFGAKLFGKKTSADGHHAG
                     IPTVAMAGVGAGGATS"
BASE COUNT          583 a          674 c          855 g          626 t
ORIGIN      
        1 tggcctgtgg tcacaatctc attcggaccg tccgccaccg tactggaaac agtaagtgaa
       61 agcaatcacc tggcggaggt aaagcaaggt cggaatctat taggagttat tcataggcct
      121 tccttatcgc gggagtcatg agaggaggac ttttcctctt tgaagcctat tgaaagagta
      181 gtgtaatatg aggtgttcga tcatgtgaag atgctcgttc caacagcact ctcgatgaga
      241 gtcacattct ggaggaattc agttatggca aattggagtc agcaagcgac gaagatgctt
      301 atcggcagcg gactgattgt atgggctctg tccggcagtg gatgcgtgtc acccgcatcg
      361 gctgcagggg tccctccagc atgggctcaa ggcttttcag atatcgtgaa aaggaccact
      421 ccggccgtgg tgaatattgc ggtgacgggc ggaggagaag ggagtcggag gcgaggtggg
      481 gttcctccga gcccattcgg aacccctccc cctggagatg aaccgggagg aggagaattg
      541 ccgactccgc cgccgattcc gcatggtcca cccatgccgc atggccgccc ggatcagagc
      601 gcgggttccg gcgtcgtctt ggattccaac gggtacatcg tgaccaataa tcacgtcgtt
      661 gaaggagcca ctcagattac tgtgaccctc agcgatcgac gcgagttttc ggcgaaggtg
      721 gtgggcacgg atccgaagac ggatttggcg gtcatcaaga tcgaggcgaa ggatctgccg
      781 tcgctgaaat gggctgagta cgacaagtta caggtgggtg atctggtgtt ggcggttggg
      841 agcccctttg ggctgagttc aaccgtcacg ctcggcatca tcagcgccct tgggcgcggg
      901 aatgtcggaa ttgcggacta tgaagatttc attcagaccg atgcggcgat caaccccgga
      961 aattctggcg gagcgctcgt caacatgaat ggagatctga tcggtatcaa caccgcgatc
     1021 ttctcgcgaa ccggcggatc ggagggaatc gggttcgcga ttcccagcag catcgccctc
     1081 gatatcgtcg aaagcctgca gagaacgggc aaggtcgtac gcggatggat gggcgtcgcg
     1141 atccaggaaa ttacgccggc cttggcaaaa tcgttcaagc tcccggagca gcggaaaggt
     1201 gtgttgatca gcgacgtcaa tgaaaatggc ccttctcatg ccgctggggt gaagcgaggc
     1261 gatgtcgtcg tggcatttaa cggcaaggac gtccaaagtg tcagtcagct gcgcaatctc
     1321 gtcgcaagaa cggtggtcgg caaagacgca caggtcaagg tcctacgcga aggcaaggaa
     1381 cagctgatcg tcgtcaaagt tgccgaacgc ccgtcggatg aaatgctggc caagaaagaa
     1441 ccggcgccgc cgaaggagcc gggagaaacg atgaagctcc ctgacaatgt gctggcgtca
     1501 ttgcgtgtcc aaacgctgga taatgcgttg atgagtcagt tgaacatttc cgcgaagacg
     1561 gccggagtgg tcattacgtc ggtcgactct ggcggaccgg cagaggcggc agggctccag
     1621 cgcggtgacg tgatccaaga agtcaatcat gagtcgatca aatcgctcag cgattatcaa
     1681 aaggctgcgg aaaagatcaa gaaagacgaa cttgccgtgc tgttggtcaa tcgacaaggc
     1741 aacagcctgt ttgtggcgat taatccaaga taggaagcac agaggcattt ggtggtctgt
     1801 cgattgccgg aagcctgatc gtcagatcgt cggatcgaca ggttccgaaa cttacgaggc
     1861 tgtgtgctga acaagctgaa ccggtggcta caagattcgg tcttcaagcc gctggaagac
     1921 aagaagatgc cggtcatgga gcacctggtg gagttccagg tgcggctcac ccgtgcggtg
     1981 atcactctgg cggtcatttt catggggacg tttttttatg ccgacgcgtt ggtcaagtgg
     2041 ttgagggttc cgcttcagaa tatgttcgtg ccgagcaaat taacctggga gcccaccgat
     2101 ttgccgacgg tgccgtttgt cttccttgcc ccggcggagg cactctggca gaacgtcaag
     2161 gtggcgggac tgttcgccat cgtgcttgcc atgccctatc tcttgttcga gatctggagg
     2221 tttgtcgttc cgggactgca cgtgcaggag cgacggtttg tcgggccgtt cgtctgtgtg
     2281 agtgcattgg cattctacgc aggagtcgga ttctcatttt tcttcgtgct tccattcgcc
     2341 ctgaattttt tgatttcgta cggggtgaac gccggattcg tgccgcagat ttcgatcgcc
     2401 caatatgtcg gttttgccct gtggttctta acggtctttg ggctgatctt tgaagtgccg
     2461 ttgaccctca cactcatggc aaagttgggt tgggtcgatg cgccgtttct gattcagtat
     2521 tggaagtggg cgttgttggg atcgtttgtt atcgcggcga ttctgacccc cacgcctgat
     2581 ccgtttaatc agacgctgat ggccggcccc atgtttcttc tctactgggt cggcatcttc
     2641 ggcgcgaagt tgttcggtaa gaaaacatct gctgacggtc atcacgcagg tattccgaca
     2701 gtcgccatgg ccggggtagg ggctgggggg gccacctc
//
LOCUS       DATNDD010000361         4714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3326719, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000361 DATNDD010000000
VERSION     DATNDD010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326719"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..817)
                     /locus_tag="VN647_08720"
     CDS             complement(188..817)
                     /locus_tag="VN647_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97151.1"
                     /translation="MRQRFKRIIHGSRIYDWINGIRNWQEWRLWRRGRLKHIPHYVKR
                     DILRHYARMYRLGSFVETGTYFGDMVDALKGDFEQIQSIELNDFLFERAKRRFMSYSH
                     IRILHGNSASMLPLALASIKQSTLFWLDAHYSGGITACGDQHSPVLLELEHIFRHPIR
                     RHVIVIDDARLFGADSAYPTLEQLQLFIDAQSTPRKLEIIDDAIRIYEV"
     gene            complement(849..1436)
                     /locus_tag="VN647_08725"
     CDS             complement(849..1436)
                     /locus_tag="VN647_08725"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF268 domain-containing protein"
                     /protein_id="HWG97152.1"
                     /translation="MATPFDPHYFFQAAWLARRLHEKRPSLHVDVGSSAMMINVLSAD
                     VKTVFVDYRPLHVHLSNLSSLAGDILWLPFQDGSITSLSCLHVLEHVGLGRYGDPINP
                     DGSLLAAAELQRVLKPGGRLFLSVPVGRERVCFNAHRVFSSNTVRDFFQELRLKAFSL
                     VDDAGRFNEEAPPETAAALEYGCGLFEFVKTREGG"
     gene            complement(1688..2422)
                     /locus_tag="VN647_08730"
     CDS             complement(1688..2422)
                     /locus_tag="VN647_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97153.1"
                     /translation="MTEHLDFQHGQVYFHGAPLGSHNDYMHSSTILTRMKHLLECLPY
                     LRCFFVLGDLPELRNISTLVPGVDVRAIPFPSLSDGPFEAVGTSFDTVHDLVFTGAMT
                     EYRARLLSMLEAGGLSVICPRKFVSRNRRNALNRLGKVIVNIPQRGGWQWLSLMRIIA
                     GLQTGRATVSLGTQDASHIASCCTQLDIRDHDWMSKVKQCVDDWKSLYMRDFISYAAM
                     MQKFEREKAFPHDVFEYWSATDRVCQ"
     gene            complement(2650..>4714)
                     /locus_tag="VN647_08735"
     CDS             complement(2650..>4714)
                     /locus_tag="VN647_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97154.1"
                     /translation="SVRRTVIEKAKREERRHDPLDRSSIAKRYQEYFWPPDHVLTAAL
                     EGDLEFHHTAPFPSLKRPFVFHCESFAPVLFPFAQQRSGSVENYEELKQHYRGIFSHP
                     LCLGVFSHVPDTLHALRLFLSDPTIDGKLFSSRTGLSADPLFLHEPELKPSLSRPRFL
                     FVNSANQNPVNFFRRGGHLVLRFWKEMVADGRDGLLMLRCTMPDDIELRDHGVDVSWV
                     KGEIGRSIVWDQGYLAAHEIQSLMAGAHFLLLPSVALHSVSIMEAMRAGAIPVVSDIV
                     GISVYVTDEENGIVLYGVRKKVRPEDGGTDCLMDQYSRWPELDNLLVEQMTSRVCALL
                     DEPGVYWDMHRRTLSHAKERFSGQSFSLEFWSSVSDLYERFRPASIAGEAGSDRANRS
                     LNECTIQRDGWARVFESPPQPMLRIKTEFGMVWELGGAMIQTYGNPRIELNDWSVLAQ
                     YYKPDAPQVTYADTLEKLEGTYLHPLGGRREGVRRRLVRWISKILRPFPAFYRCAAQF
                     LAVYRRHGGFRFAKPTAEPEIELVRQGVTGYNIIRHRDRYYAILQREGEFSPKKAEAG
                     GYSSCYRGYSVDDVLRSIAASIPASRSFAREEGVESVEVIVEGFHNFNIVRQGKEFYA
                     FLQSESESARSKLLSKQYAPSFSGVSLEEVQRKILSALTAESGWLQGWENPVDSIETS
                     RRGIR"
BASE COUNT         1256 a         1295 c         1159 g         1004 t
ORIGIN      
        1 cgccgcacga cgttataggc gagcagttca ctctgagcca gccaggcccg tagaccgcta
       61 ctcacgcgcc ttcacaaatt cgaaaagacc acacccatac tcgagggaag ctgccgcatc
      121 cgagacgacg accttcaata accaaggtgc ccggcaagca gcggctgtgc ggagcgaccc
      181 ccttgcgcta gacttcataa attcggatgg catcgtctat gatctcaagc tttcgaggag
      241 tggattgtgc atcgataaaa agttgcaact gctcaagggt cggataagcc gaatccgcac
      301 cgaagagtct tgcatcatca attacaatca catgccttcg tatcgggtga cggaaaatat
      361 gctcgagctc aaggagtacc ggcgaatgct gatcaccaca ggctgtaata ccaccggaat
      421 aatgagcgtc tagccagaac agtgttgatt gcttgattga tgccaatgcg agaggcaaca
      481 ttgatgcgct atttccatga agaatcctga tatgcgagta gctcataaag cggcgtttcg
      541 cccgctcaaa gagaaagtca ttgagctcaa tggactggat ttgctcaaaa tctcccttca
      601 gagcatctac catgtcacca aagtatgtcc cagtctctac aaaagaccct agacgataca
      661 tccgcgcgta gtgccgaaga atatcccgct tcacataatg cggtatatgc tttaatcgcc
      721 cacgccgcca gagccgccat tcttgccaat ttctgatccc gttgatccaa tcataaattc
      781 tagaaccatg aataattctc ttgaaccgct gtctcataag acttccatat aatcactcac
      841 cctcccgatc acccaccctc acgtgtcttc acaaattcga acagaccgca tccatactcg
      901 agagcggcgg cagtttccgg cggagcctcc tcgttgaatc gtccggcatc gtccacaagg
      961 gaaaaagctt tgagccggag ttcctggaag aaatccctga cagtattcga tgaaaacact
     1021 cgatgcgcat tgaaacagac ccgctcgcgt ccgaccggta ccgataagaa gagtctcccg
     1081 cccggtttca atacgcgctg tagctccgca gcagccagaa ggcttccatc cggattaatg
     1141 gggtccccat atcgaccgag tcctacatgt tcgagaacat ggaggcatga caatgaggtg
     1201 atgctgccgt cttgaaaggg cagccacaag atatcgccgg caagggaact cagattagag
     1261 agatgcacat gaagaggacg atagtcgaca aacacggttt tcacatcggc actcaacacg
     1321 ttgatcatca tggcactgga cccgacatcg acatggagag aaggccgctt ctcatgcaat
     1381 cggcgcgcaa gccacgcagc ctgaaaaaaa tagtgcgggt caaacggcgt ggccacggtg
     1441 cgatcagcaa gacatggata tgaatctcga aaagagatct gtcgtgtggc ggtcttccta
     1501 ttatagattt tccattccgc gagatagcga ggcaatgctc caaggctggt aaggcagtga
     1561 gaaccaagaa aagctaaacc ccacctgacg attgcatggt tgcggaaaag ttgtctcacg
     1621 acaaaggcgg ggagcgacga tcgctaatag aagcagcgcg aggacggtga acagagatag
     1681 agatgtacta ctggcacact cgatcagtgg cactccagta ttcgaagaca tcatgcggaa
     1741 acgctttttc ccgctcaaac ttctgcatca tggctgcata gcttataaag tctctcatat
     1801 aaagcgattt ccagtcatcg acgcactgtt tcaccttact catccaatca tgatcccgaa
     1861 tatccaactg tgtacagcag gaagcgatat gagaggcatc ctgtgttccc agtgagacag
     1921 tggctcgacc ggtttggagt ccggcgataa ttcgcatcaa tgacagccat tgccaacctc
     1981 cccgctgagg aatgttcaca atcactttcc ccaatcgatt gagggcgttg cggcgattcc
     2041 ttgatacaaa tttcctcgga catatgaccg acagtcctcc cgcctctaac atagaaagaa
     2101 gccgggcacg gtattccgtc atggctccgg taaacacaag atcgtgaacg gtatcaaaag
     2161 acgttccaac agcttcgaat gggccgtcgc ttagggaggg aaatggaatg gcgcgcacat
     2221 ccacaccagg tacaagcgtc gagatgttgc gtaactcagg caagtcgccc aaaacgaaga
     2281 aacagcggag atagggcaag cactccaaca gatgcttcat ccgtgtcagg atcgtggagg
     2341 agtgcatgta atcgttgtga ctaccaagag gggcaccgtg gaaatagacc tggccatgct
     2401 ggaaatccaa atgctcggtc atgatcactg cgactcgttt ccgtgatgat cggcaaaaat
     2461 tcagcaacac atccctactg ctttccgagg gaaacccttc aatgacgaca ttcagagatg
     2521 aacggcgcgg ctgtctccca acggaaaccg gatagccgtg ttgtcggaaa gcagtgaccg
     2581 caaagctcac ttggtcaccg atcccataca attgatgatt gtagacccaa caatggattg
     2641 aaggcttctt cagcggatgc ctcgtctcga cgtttcgata gaatcgacgg gattctccca
     2701 gccttggagc caccccgact ccgcagtgag tgcactcaaa atttttcgct gaacctcttc
     2761 aagcgagaca cctgaaaaag agggggcata ttgtttcgaa agtaatttcg atcgcgccga
     2821 ttcgctttcg ctttgcaaga aggcgtaaaa ttccttgcct tgacggacaa tgttgaaatt
     2881 atgaaacccc tccacgatga cctcgacaga ttcaacgcct tcttcgcgag cgaacgacct
     2941 cgatgccgga atacttgcag cgatacttcg gagcacatca tcaacagaat accctcgata
     3001 acacgacgaa tagccgccgg cctcggcttt ctttggagaa aattcccctt cgcgctgaag
     3061 aatggcatag tatcgatcac gatgacggat aatgttatac ccggttactc cctgacggac
     3121 gagttcgatc tccggttccg ccgtgggctt ggcgaatctg aaccctccat gccgacgata
     3181 caccgctaga aactgggcgg cacagcgata gaaagcggga aatggcctaa gaattttgga
     3241 aatccatcga accaacctgc gccgcactcc ttcgcggcgc cctccgagcg ggtgaaggta
     3301 ggttccttcc agtttttcca aggtatcggc atacgtcacc tgtggagcat cgggcttata
     3361 gtactgggcc aatacggacc agtcattcag ctcgatgcga ggattcccat acgtctggat
     3421 catggcgcca cccaattccc ataccatgcc gaactctgtc ttaattcgta acatcggctg
     3481 tggggggctc tcaaacactc tcgcccagcc atcacgctga atcgtacact cgttcaagga
     3541 acggttcgcg cgatcggaac cggcctctcc ggcaatggac gctggtctga atcgctcata
     3601 gaggtccgac acggagctcc agaattccag agaaaaactc tgccctgaaa atcgttcttt
     3661 tgcatgggaa agcgtgcgcc tatgcatgtc ccaatagacg ccaggctcgt ccagcaaggc
     3721 acacacacgg cttgtcattt gctcgacaag aaggttgtca agttccggcc atcggctata
     3781 ttggtccatc aaacagtccg ttcccccgtc ttcaggccgg actttctttc gtacaccgta
     3841 caagacgatc ccattctcct cgtcagtgac gtacactgag atgcccacga tatcagacac
     3901 gaccggaatc gctcccgccc tcatggcctc catgatcgag acagagtgca gggccacgct
     3961 gggaagcagg agaaaatgcg cgccggccat taaggactgt atttcatggg cggccaggta
     4021 gccttgatcc cagacgatac ttcgcccaat ctcacctttg acccaggaca catcaacacc
     4081 atgatcacgc aactcgatat catcgggcat cgtgcaacga agcatcagaa ggccgtctcg
     4141 gccatcggca accatctctt tccagaaacg taagacgaga tgcccacccc gacgaaagaa
     4201 attcaccgga ttctggttgg cggagttgac aaacaagaac cgcggcctgg aaagcgacgg
     4261 ctttagctcc ggttcatgca gaaataatgg atcggcactc aatcccgttc ttgaggaaaa
     4321 caacttcccg tcgatggttg ggtcggataa gaacaggcga agcgcatgaa gcgtgtccgg
     4381 cacatgggaa aagactccga ggcacagagg atgcgagaaa atgcctctat aatgttgctt
     4441 gagttcctca tagttttcaa cactgccgct ccgttgttga gcaaacggga acaagaccgg
     4501 cgcaaacgac tcgcagtgaa agacaaacgg tcgtttgagt gaggggaatg gggcggtatg
     4561 atgaaattcc aggtccccct caagagcggc cgtcagaacg tgatcaggag gccagaaata
     4621 ctcctgatac ctctttgcaa tagaacttcg atcaagtgga tcatgacgac gttcttcccg
     4681 ctttgctttc tctatcactg tcctgcgcac agaa
//
LOCUS       DATNDD010000362        10290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2158310, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000362 DATNDD010000000
VERSION     DATNDD010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158310"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /gene="thrS"
                     /locus_tag="VN647_08740"
     CDS             <1..1683
                     /gene="thrS"
                     /locus_tag="VN647_08740"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWG97155.1"
                     /translation="ELFPSAQLTIGPALEDSFYYDFAFERPFTPEDLERIEERAAAII
                     KRNLTITRREFSKQDAIDFFRSRGEHYKVELIQGFPDGESITAYTQGDFVDLCRGPHL
                     RTTGSVGAIKLLTTAGAYWRGDERNPMLQRIYGTSFPTRAEVDAYLARLEEIKRRDHR
                     KVGKELDLISIQDEIGPGLVLWHPKGAAVRLLIENFWREQHIRDGYQLVYSPHTARLD
                     LWKTSGHLDYYRENMFPSMKLEGSEYQLKPMNCPYHIMIYQSHLRSYRDLPIRYGELG
                     TVYRYERTGVLHGLMRVRGFTQDDAHLFCRPDQMEHEVSRVLDFTFFVLQTFGFQQFE
                     VFLSTRPKESVGGDEHWILATSALEAALKSRNISFHLDPGGGAFYGPKIDIKIKDALG
                     RSWQCSTVQVDFNNPERFELSYIGEDGKAHRPIMIHRALMGSIERFFGILIEHYGGAF
                     PTWLAPVQAVVMNITDHQQDYVAAVVAQLKRAGFRAEADLRNEKIGFKIREAEKAKIP
                     FMLVAGNREMQDRTLSVRGRSGSNLGNKTVAEVLDLLRAEVIHTQRELQPTH"
     gene            1716..2207
                     /gene="infC"
                     /locus_tag="VN647_08745"
     CDS             1716..2207
                     /gene="infC"
                     /locus_tag="VN647_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWG97156.1"
                     /translation="MNREIRVREVRVIGPEGEQLGIIPTPDAFRQAQENGYDLVEVAP
                     NSVPPVCRIMDFGKYKYELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIKVRQMKV
                     FLEEGNKTKVTLTYRGREMANQEMGRAVMNSVIEQLAQTGTIEYAPRMEGRSLIMIVA
                     PKH"
     gene            2247..2441
                     /gene="rpmI"
                     /locus_tag="VN647_08750"
     CDS             2247..2441
                     /gene="rpmI"
                     /locus_tag="VN647_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWG97157.1"
                     /translation="MKMKTHKGTSKRFTKTGSGKIVRRKAGKRHLLTHKRHDHKRRLS
                     GTTVVDPTVVSSLRRLLPYE"
     gene            2496..2864
                     /gene="rplT"
                     /locus_tag="VN647_08755"
     CDS             2496..2864
                     /gene="rplT"
                     /locus_tag="VN647_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWG97158.1"
                     /translation="MPRAKGGPKTRQRRKKRIKLASGQYGGKSRLFRSATESVDKGLT
                     YAYTGRKNRKRDFRQLWIARISAAVRAQGMTYGRFINALKKANIALDRKVLSDMAIKD
                     ATGFQELIGLAKQHLTPVTA"
     gene            complement(2952..4670)
                     /gene="pheT"
                     /locus_tag="VN647_08760"
     CDS             complement(2952..4670)
                     /gene="pheT"
                     /locus_tag="VN647_08760"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWG97159.1"
                     /translation="MPTITIFQDDLESLLTGDRGAGRGSTMAQLEEWLMLVKGELKGH
                     NPETGELRIELQDSNRPDLWCCEGIARQIRIKQQGRLKPYSFFAKKPKSPHHLIVKPG
                     IEQVRPYVAACTAKGYRVTEKGLAQLIQTQEKLADIFGRKRKTVSIGIYQLRNIAFPV
                     TYELVKPDEVRFTPLGMETTMTLSEILMVHPKGLEYGPILAVKSLLPILRDAKDQPLS
                     FPPIINSREIGEVRLGDDELFIEVTGTDLPMVILSLNIFAANLADRGASIEPVEIQYP
                     TSTMLGKRVKTPQDFGKPKVIRIKTIEQALGQELGETVVKQALEAYGYDVSAEKGSVK
                     AKLPPYRHDLMHTMDVVEDVAMSRGYAEFTPVMPAQFTVGGLSAIEQLSDRSRELMVG
                     LGFQEIISNILGSPDHYSRLMRLEGTEWGRMVEVDNVMSLSFSCLRQWMLPSLLRIEA
                     ASSRAFYPHRLFEAGDVAIPDGAHELGSRTETVLGALIAHVTTHFSEIHSGLDVLFYY
                     LGKEYSLEPMQHPSFLEGRAGRILVSGKPVGVIGEIHPEILEHWQITMPVVAFDVNLS
                     QLAMFG"
     gene            complement(4679..6244)
                     /locus_tag="VN647_08765"
     CDS             complement(4679..6244)
                     /locus_tag="VN647_08765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWG97160.1"
                     /translation="MDISAVIDSLHPLEIKVLMTLGARPPGTALDSEQLTTAAELEPS
                     QLSMAVEWLLAKSLITVQRETVTPIVSLTKIGEEYYQTASPIERVLMAAREATGTGTR
                     LTIPDLQAQGGLDPSEVSKAVGRLKKEGVVLIIQGGCIETTGRSSPTVEALRVLLGEM
                     HDSPKELKSFTEAHRQVIEDFAVKRGNAKEPFRVDERVVRSFTLSPDGAGAAEQLLRQ
                     GIAEEVSQLTPDLLKDGSWRQKRFRKYTISLRPPRIGTGKKHPYREFLDTVKAKLVSM
                     GFLEMRGPLVETEFWDMDALFMPQFHPARDIHDVYFVKKPSHTTAVDEPFLSRVAKVH
                     ENGAETGSTGWGYSFDVHRAKRLVLRSQGTAVSARTLAAMPNIPGKYFSIARCFRYDQ
                     VDATHATDFFQVEGIVLGEDINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVE
                     LHVRHPRLGWMELGGAGLFRAEVTLPLGVTVPVIAWGLGLDRMAMVALGIHDIRELFT
                     DNLELIRTTRGLF"
     gene            complement(6255..6956)
                     /gene="rpe"
                     /locus_tag="VN647_08770"
     CDS             complement(6255..6956)
                     /gene="rpe"
                     /locus_tag="VN647_08770"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWG97161.1"
                     /translation="MGHPIYIAPSILSADFAHLADEVAAVERAGADLLHVDVMDGHFV
                     PNLTVGPPIVESLKKVTKLPLDVHLMITNADMFIPEFAEAGADYLTVHVEACPHLHRT
                     IQSIKERGVKAGVTLNPGTPLSLLQEILGDVDLVLIMSVNPGFGGQAFIPSVLRKIAE
                     ARVMLDAINSHALLEVDGGVKVDNAREIVAAGATALVAGSAIFSQHDYTATIAAMRAA
                     AETVVQQAPRVAVNR"
     gene            complement(6962..8368)
                     /gene="rsmB"
                     /locus_tag="VN647_08775"
     CDS             complement(6962..8368)
                     /gene="rsmB"
                     /locus_tag="VN647_08775"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWG97162.1"
                     /translation="MDSDAPGPSSRAVALSILLLSRRTDDAVDELIEQQTTSVSQLRD
                     RSLVMELVYGVLRRQETIDWRLDAVLSKPLHRLPVVVQVLLRLGAYQLLFLDRIPASA
                     AVNETVRLTKSYVKQLRRDWSGLVNGVLRNLIRVPTPSFPDPAVYPARSLSVRYGIPE
                     WLTERWLDRMGLEQAESACRAGGTIPSVTLRVNSNRLTREEFLQRLRQAGVTTYPTVV
                     SSVGVVLEKSQDVTSLPGFQTGDFYVEDEAAQLIPPILDPQPGETVLDACAAPGGKTT
                     HLAELMGDRGTIYAVDRRAPRLDLLQQNCRRLGIENILPIVGDVRKPSEWSRVVARGL
                     NLREGSLLFDRILVDAPCSGIGVLRRHPEAKWRKDSSAFARHQKLQAEILTSVAPSLR
                     PGGVLVYSTCSTETEETEGVVRRFCETYPGWMRESVAPWLPTTALPFVTIYGALSTMG
                     NECGMDGFYAARLRKKEG"
     gene            complement(8420..9361)
                     /gene="fmt"
                     /locus_tag="VN647_08780"
     CDS             complement(8420..9361)
                     /gene="fmt"
                     /locus_tag="VN647_08780"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWG97163.1"
                     /translation="MRIVFMGTPEFAVPSLEALLRSNDQVVGVVSQPDRPKGRGQQLV
                     PPAVKLVAEQAEIPILQPLKIRAPEFLQALSAWQPDLIAVAAYGRILHAPILQLPPMG
                     CVNVHGSLLPKYRGAAPVQWAVIHGETETGITTMLMNEGMDTGPILLQGKLKISPDDT
                     AGTLSPRLAELGGRLLVDTIAQLKAGTLVPKKQDDGQATLAPLLKKEDGLIDWTMNAT
                     ALANRVRGLSPWPGAYTFFGAERWNIWKATSNAGAVTDKPGTIVVANKQAIVVATGDG
                     LLDIREIQTANSKRMSVGQFLAGHQVTTGGQLGSSVT"
     gene            9449..9525
                     /locus_tag="VN647_08785"
     tRNA            9449..9525
                     /locus_tag="VN647_08785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9483..9485,aa:Met,seq:cat)
BASE COUNT         2463 a         2873 c         2676 g         2278 t
ORIGIN      
        1 gaactcttcc catcagcaca actgaccatc ggcccagccc tagaagatag tttttactat
       61 gactttgcct tcgaacgtcc ctttactcct gaagacttgg agagaatcga agaacgcgcc
      121 gcagcaatca tcaagcgtaa tctcaccatc acaaggcggg aattttcgaa acaggacgcg
      181 atcgattttt tccgatcccg tggagagcac tataaggtcg agctgattca gggttttccc
      241 gatggagaat cgatcaccgc ctatacacaa ggcgatttcg tcgatctctg tcgaggcccc
      301 catctccgga ccaccggctc cgttggggcc ataaaattgc tcaccacggc cggagcctat
      361 tggcgtggcg atgaacgtaa cccgatgtta caacggatct atgggacatc ctttccaacc
      421 agagcggaag tggatgccta tctggcccgg ctggaggaaa ttaagcggcg tgatcacaga
      481 aaagtcggaa aagagctgga tttgatcagc attcaagatg agatcggacc cgggctggta
      541 ctctggcatc cgaagggtgc ggcagtccgc ctgttgattg agaatttctg gagagaacaa
      601 catattcgag atgggtatca gctggtctat tcgcctcata ccgcacgtct tgatctctgg
      661 aaaaccagcg ggcatctcga ttactaccgc gaaaacatgt tcccatcgat gaagctggaa
      721 ggcagcgagt accagctgaa accgatgaat tgtccgtacc atatcatgat ctatcagtca
      781 cacctgcgaa gctatcgaga cctccccatt cgctatgggg aactaggaac ggtctaccgc
      841 tatgaacgga ccggcgtact gcatgggctc atgcgagtgc gcggattcac acaggatgat
      901 gcccatctct tctgccgccc ggatcagatg gaacacgaag tcagccgggt actggacttt
      961 acatttttcg tattacagac attcggattc cagcaatttg aagtgttttt gtccacacgg
     1021 cccaaagagt ctgtgggtgg tgatgaacat tggatcttgg ccaccagcgc attagaagcg
     1081 gcgctgaaaa gccgcaatat ttcatttcat cttgatccgg gagggggagc attttacggc
     1141 ccgaagatcg acatcaagat caaagatgcg ttgggccgtt catggcaatg ctccacggtc
     1201 caggtggatt tcaacaatcc agagcggttt gagttaagtt acattggaga ggacggcaaa
     1261 gcccatcgtc ccatcatgat ccatcgcgcc ttgatgggat ccatcgaacg ttttttcggc
     1321 attctgatcg agcactatgg gggtgcgttc ccaacctggc tggctccggt gcaggcggtc
     1381 gtcatgaaca tcaccgatca tcaacaggac tatgtcgcag ctgttgtcgc ccagttgaag
     1441 agggccggct tccgtgctga agcggacctt cggaacgaaa aaatcgggtt caaaattcgt
     1501 gaggcggaaa aggcgaaaat tccgttcatg ttggtggcgg ggaatcgcga aatgcaagat
     1561 aggactctct cggtacgtgg ccgaagcggg tcaaatttag ggaataagac agtggctgag
     1621 gtgttggatc tcctgcgagc cgaggtcata cacacacagc gagaacttca acccacacat
     1681 taaaaaagag gtggcctatc gttcccaaac tacgcgtgaa tcgtgagatc cgagtccgag
     1741 aagttcgtgt gattggcccg gagggagaac aactcggtat cattccgaca ccggatgcct
     1801 tccgtcaggc tcaagaaaac ggctatgact tggtggaggt tgctcccaac tcggttcccc
     1861 ctgtctgtag aattatggac tttgggaagt acaagtatga gctgagtaaa aaagatcatc
     1921 aaagccggcg tcatcaaaag tccacccaag tgaaggaaat caaattgcgt ccacggacgg
     1981 ataagcatga ccttgaaatt aaggtcagac agatgaaggt ttttttagaa gagggcaaca
     2041 agaccaaggt caccctcacc tatcgcgggc gggaaatggc caaccaagag atggggcgcg
     2101 cggtgatgaa ttccgtcatt gaacagttgg cccaaacagg caccatcgag tatgctcctc
     2161 ggatggaagg acgcagtttg atcatgatcg tcgctccgaa acattgacga acagcacggt
     2221 gattgctcaa ccaaaggaat ctcctcatga aaatgaagac gcataaaggg actagtaagc
     2281 gtttcacaaa gaccgggagt gggaaaatcg ttcgccgtaa ggccggcaaa cggcatctgc
     2341 tgacccacaa acggcacgac cacaagcgcc gattgagcgg aaccacggtc gtcgatccga
     2401 ccgtcgtctc gtcgttgagg cggctgctcc catacgaata acacgatcat tgccacggag
     2461 aagaacatca ggaccctata aggagtaatt gaattatgcc tcgcgcaaag ggtggaccaa
     2521 aaactcgaca gcgaagaaag aaacggatca aattggcatc aggtcagtat ggcgggaaaa
     2581 gccgcctctt tcgttcggcg acagaaagcg tggataaggg cctgacctat gcctacacgg
     2641 gtcgcaagaa ccgaaagcgg gacttccggc agctatggat cgctcgtatc agcgcggcag
     2701 ttcgagcaca agggatgacc tatggccgat tcatcaatgc cctcaagaaa gccaatattg
     2761 ccctcgatcg caaggtcctc tcggacatgg cgatcaaaga cgccacgggg tttcaagaac
     2821 tcataggcct cgccaagcaa cacttgactc ccgtcacggc ctaacattca agagtccaat
     2881 aggttgtcaa tggaagggga aacgacagtt tgccgtttcc ccttccattg tttcagacga
     2941 aggaacaatc cctacccaaa catcgccaac tgtgacaggt tcacgtcgaa cgcgacgacc
     3001 ggcatggtga tctgccaatg ttcgaggatt tctggatgga tttcgccgat cacgccgacg
     3061 ggcttgcccg agacgagaat ccgaccggct cggccttcta aaaaagacgg atgctgcatc
     3121 ggctcaagac tgtactcctt accgaggtag taaaacagaa catcgaggcc ggaatggatt
     3181 tctgagaaat gggtggtgac gtgcgcaatc aaggcgccca agacagtttc cgtgcgagac
     3241 cccaactcgt gggcaccatc ggggatcgcc acatcaccgg cctcgaagag gcgatgagga
     3301 tagaaagccc ggctcgaggc ggcttcgatg cgcagcaatg agggcagcat ccattgtcgg
     3361 agacaagaga agctcaacga cataacattg tcgacctcaa ccattcgtcc ccattccgtt
     3421 ccttccaggc gcataagacg cgaataatga tccggtgaac cgagaatatt cgaaatgatt
     3481 tcctgaaacc ccaaccctac catcaactct cgggatcgat cggacagctg ttcaatggcg
     3541 gataatcctc ccaccgtaaa ttgcgccggc atgaccgggg taaattctgc atagccgcga
     3601 ctcatggcga cgtcttcaac cacatccatc gtatgcatca gatcatgccg gtacgggggg
     3661 agtttggcct taaccgatcc cttttctgcc gacacgtcgt acccatacgc ctcaagcgct
     3721 tgcttcacga ctgtctcgcc tagttcctgg cccagcgcct gttcaatcgt tttaatccga
     3781 atcactttgg gtttcccgaa atcttgtggg gttttgaccc gtttgcccag cattgtgctc
     3841 gtcggatact gtatttccac cggctcgatg gatgcgccgc ggtcggccaa attcgccgcg
     3901 aagatattca gtgaaagtat caccatgggt aggtctgtcc cagtcacctc aatgaagagt
     3961 tcgtcatcac ccagccgtac ttctccgatt tcccggctat taataatcgg cggaaacgac
     4021 agcggttggt ccttcgcatc tcgaaggata gggagcagac tcttgaccgc cagaataggc
     4081 ccgtactcca accccttggg gtgaaccatg agaatttccg acagggtcat cgttgtttcc
     4141 atacccaatg gggtaaaccg gacttcatca ggtttcacca actcataggt cacaggaaag
     4201 gcaatattcc gcagctgata gattccaatg gaaacggtct ttcgcttacg cccaaagatg
     4261 tcggccagct tctcctgcgt ttgaatcagc tgagccagcc ccttctcggt cacccgatat
     4321 cccttggccg tacaggctgc cacgtacgga cgcacctgct ctataccggg cttcacgatc
     4381 agatgatgcg gcgacttcgg ttttttggcg aagaaggaat agggcttcag tcggccttgt
     4441 tgcttgatgc gaatttgcct ggcgatccct tcgcaacacc agagatcggg ccgattgcta
     4501 tcttgaagtt cgatgcgcag ctctccggtt tcagggttgt gccccttcag ctcgcctttc
     4561 acgagcatga gccactcctc caactgagcc atcgtgctac cacggccggc accgcgatca
     4621 cccgtgagaa gcgactctaa atcgtcttga aaaatagtga tcgtaggcat gagcgcagtt
     4681 agaacagacc tctggtcgtg cgaatcaatt ccaggttgtc ggtgaagagt tcgcggatgt
     4741 catggatgcc caacgccacc atcgccatac gatcgagccc caacccccag gcaatgacgg
     4801 gcacagtgac accaagcggc aacgtcactt cggcacgaaa gagacccgcg ccgccgagtt
     4861 ccatccatcc caagcgtgga tgccgtacat gcagctcgac cgacggttcg gtaaacggaa
     4921 aatatgccgg taggaacttt acttccttcg cttgtgccac ttccctggca aacaaattca
     4981 agagacccaa cagcgtccta aagttgatgt cttctcccag cacgattccc tccacctgga
     5041 agaaatccgt ggcgtgggtc gcgtcgactt ggtcatagcg aaagcaccgg gcgatcgaga
     5101 aatacttccc gggaatgttc ggcatggcgg caagcgttcg agccgaaact gccgtgcctt
     5161 gactccgcag caccaaccgt ttggctcggt gaacatcgaa tgaatagccc cagcctgtgg
     5221 aaccggtttc cgcaccgttc tcatgcacct tggcaacacg cgacaaaaac ggctcgtcca
     5281 ctgcggtggt atggctcggc ttcttgacga aatagacatc atgaatgtcc cgggccggat
     5341 ggaattgcgg catgaacagg gcatccatat cccagaactc cgtttcgacc agcggcccac
     5401 gcatctctag gaaccccatg ctcacaagtt tggctttgac cgtatcaaga aattctcgat
     5461 agggatgttt ctttccggtt ccgatacgag gcgggcgcag actgatggtg tactttctaa
     5521 atcgcttctg acgccaactc ccatccttca acaagtcagg agtcaactgt gatacttctt
     5581 cagcaatccc ctgtcttagc agctgctccg ccgcaccggc accgtccggt gacaacgtaa
     5641 atgagcggac tacccgctca tccacccgga acggctcttt ggcattgccg cgtttgacgg
     5701 caaaatcttc aatgacctgc cgatgagctt ccgtgaagct cttcagctcc ttcggcgagt
     5761 catgcatttc tcccaagagc actcggagtg cctcaaccgt cggactcgag cgaccggttg
     5821 tttcgataca gcctccttgg atgatcagga ccaccccttc tttcttgagc cgtccaacgg
     5881 ctttactgac ttcggaaggg tccagcccgc cttgcgcctg aagatcggga atggtcagtc
     5941 gtgtcccagt gccggtcgct tcccgcgcag ccatcaagac tcgttcgatt ggtgaagcag
     6001 tctggtaata ctcctctcct attttcgtca gcgacactat tggagttaca gtttccctct
     6061 gcaccgtgat gagagacttg gccaataacc actcgactgc catgctgagc tgggaaggct
     6121 ctaattcagc ggctgtggtc aactgctcgc tatccaaagc ggttcccggc ggacgtgcgc
     6181 ccagcgtcat gaggactttg atttcgagag gatgaaggct gtcgatgact gcggagatgt
     6241 ccaccctaaa tggcctatcg attgaccgct acgcggggcg cctgttgaac gaccgtctct
     6301 gcggctgctc gcatggctgc gatcgtggcc gtatagtcgt gttgggaaaa aatcgctgac
     6361 cctgccacga gcgccgttgc gccggcagcg acgatctccc tggcgttatc gacctttaca
     6421 ccgccatcca cttcgagcaa ggcatggctg ttgattgcat ccaacatgac ccgcgcttcg
     6481 gcaattttcc tgagcaccga tgggatgaat gcctgtccgc cgaatcctgg attcaccgac
     6541 atgatcaaca caaggtcaac atctcccaag atctcttgga gcaacgagag aggagtgcca
     6601 ggatttaagg tcactccggc cttgaccccc cgttccttaa tcgattgaat tgtccgatgg
     6661 aggtgtgggc aggcctcgac gtggacggtg agatagtcgg cccccgcctc tgcaaactca
     6721 gggatgaaca tatcggcatt ggtaatcatc aaatgaacgt cgagcggcag cttggtgact
     6781 tttttcagac tttcgacgat gggtggtccc acagtcaggt tcggcacaaa atggccgtcc
     6841 atgacatcca catggagcag gtctgcgccg gcccgctcca ccgctgcaac ttcgtcggcc
     6901 aagtgcgcga aatcagcgga aagaatcgag ggcgcaatat aaatcggatg ccccattatc
     6961 atcatccttc ctttttacga agacgagcgg cataaaaccc atccatcccg cattcgttac
     7021 ccatggtgga gagcgcgccg tagatggtta caaaggggag agcagtggtg gggagccacg
     7081 gcgccacaga ttcacgcatc catccaggat aggtttcgca aaaacggcgt acgacccctt
     7141 ctgtctcttc cgtttctgtc gaacaggtac tatagaccag caccccacca ggccgcaagc
     7201 tgggagccac cgaagtaagg atttcggctt gaagtttctg gtgcctggcg aatgccgaac
     7261 tgtccttccg ccatttcgcc tcagggtgtc gacgcaacac acctatgccg ctacaagggg
     7321 catccacgag tatacgatcg aacagtagcg atccctcacg aaggttcaac ccgcgcgcaa
     7381 ccactctaga ccattcggat ggttttctca catcgccgac gatcggaaga atgttctcaa
     7441 tccctagtct tcggcaattt tgttgcaaca gatccaaccg tggggctctt cgatcgacag
     7501 catagatcgt tccgcgatca cccatcagtt cggcaaggtg cgtcgtcttg ccgcccggag
     7561 cggcgcaggc atccagcacg gtttcgccgg gctgagggtc gaggatcggt ggaatgagtt
     7621 gagccgcttc atcttccaca tagaaatcgc ctgtctggaa gcccggtaac gaagtgacat
     7681 cctgactttt ttccagcacg actcctacag agctgacaac tgtaggataa gtcgtaacac
     7741 ctgcttgtcg caaccgttgt aagaactctt ctctggtgag tcgattagag ttcactcgaa
     7801 gtgtgacgct cgggatcgtc ccgcccgccc gacaagccga ttctgcttgt tcaagcccca
     7861 ttcgatcaag ccaccgttcg gtcagccatt caggaattcc ataccgaaca gatagagaac
     7921 gagctggata tacggctgga tctggaaaag acggcgtcgg tacacgaatg agattgcgca
     7981 ggactccgtt caccaacccg ctccaatccc gtctcagttg tttgacgtag gatttggtca
     8041 gtcggacagt ttcattcacc gctgccgagg caggaatgcg atccaaaaac aaaagctggt
     8101 aggcgccaag gcgcaacaac acttgaacga ctacgggcaa tctgtggaga ggtttggaga
     8161 gtaccgcatc aagtctccaa tcgatcgtct cttgccggcg taagaccccg tacaccagtt
     8221 ccatcaccaa ggaacggtca cgtagctgcg acaccgacgt ggtctgttgt tcaatcaact
     8281 catcaactgc atcatcagtt cgccgactca agagaaggat agagagggca accgctcgcg
     8341 acgacggtcc tggtgcgtca ctatccatat ttcgttcgtg gacctccatc tgacctagaa
     8401 agtgaatgcg gggaaaagat catgtaaccg atgaacccaa ttgcccaccg gtggtgactt
     8461 ggtgtccagc cagaaattgg ccgaccgaca tgcgtttcga attggcggtc tgaatttcac
     8521 ggatgtcgag aagaccatcg cctgtcgcca ctacgatcgc ctgcttattc gctacgacga
     8581 tcgtcccggg tttatcggtc acagcacccg cattcgaggt tgctttccat atattccagc
     8641 gctccgcacc gaaaaatgta taggccccag gccaaggaga aagccctcgc actcgattgg
     8701 cgagagccgt cgcgttcatc gtccaatcga tgaggccgtc ttcttttttc aagagcggcg
     8761 ccaaggtcgc ctgcccatcg tcttgcttct tcggcaccaa tgtcccagct ttcaattgcg
     8821 cgatcgtgtc gacaagcagt cgcccaccca actctgccag acgaggcgac aatgtccctg
     8881 ccgtatcatc aggtgagatt ttcaactttc cctgaagcag aatcggtccc gtatccattc
     8941 cttcattcat gagcatcgtg gtaataccgg tttcggtctc gccatggatg acggcccact
     9001 gcaccggcgc tgccccgcga tatttcggta gcagtgagcc gtgcacattc acgcaaccca
     9061 tggggggaag ttgaagaatc ggtgcatgca gaatgcgtcc gtaggctgcg acggcaatca
     9121 gatccggctg ccaggcagaa agggcctgca agaattccgg cgcacggatc ttgaggggct
     9181 gcaggatggg aatctcggct tgctcagcga caagtttcac cgctggaggg acaagctgtt
     9241 gtccacgccc tttcggccga tccggctgtg aaaccacgcc gactacctgg tcattagatc
     9301 gaaggagtgc ttcgagcgac ggcacggcaa attccggtgt acccatgaag acaatacgca
     9361 tgccttcgct ttagcatcgt gttcgccaga atgcaatcat gcaacacctc aacttgactt
     9421 gccattcaac cctttgttag catcccgccg cggggtggag cagcctggta gctcgttggg
     9481 ctcataaccc aaaggtcaga ggttcaaatc ctctccccgc aaccattttt tgacatcccc
     9541 ccacaagttc actctcgctc ttatataata aggcccgctg cacacccgcg agcatgtgtt
     9601 tatccaccga tagttcggct acgagccgct acaacggacc gttctgctcc agctgcgcag
     9661 gcaccggagt tcgcacgcga acgtctgctc gacgatcccg acctggcggc aagcatctcc
     9721 actgggcagg ggaaggacgt aggcgatcta ttccccagaa gactttgacc gtttgatcga
     9781 ccaagtcgct tcgtcgagga ccgttaaagt cgccattcta ctccatttag cctatcgtcg
     9841 cttttattag aggaaccgtc aacttggaga gtttgcctag ttgactcgag aacgagtccc
     9901 cataccgcat cgagatctcc gtatcgcaaa tctgcattca gatatgctta tgccgaagtt
     9961 gtccaatgta gccatggact tgaccatcgc agtcatgaag tccttatgga tagcagattg
    10021 ccctcaggtg gagacgcgca gcagagaaat acgcccttct tttggggggt ggacattaac
    10081 agtttcgata ccttagaaaa ctgatgcggt tacacagact agaggttatc tcaaaatcga
    10141 tgtttgagga ggttattctc gtgcaatgca gctattcgaa ttgcaaagaa tcgagtttag
    10201 ctcaacatat gcgtgtctgt ctctccgtgt cctgggctcg cgctcgttcg gaacgtcgat
    10261 tattcattgt gagagaggtc ctagatcaat
//
LOCUS       DATNDD010000363         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3886494, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000363 DATNDD010000000
VERSION     DATNDD010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3886494"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VN647_08790"
     CDS             complement(<1..208)
                     /locus_tag="VN647_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97164.1"
                     /translation="MTRRHKYRMIALALVHVYIAFHMIAWHIFGVQIWGKTAMMGVPS
                     LANGTINAASIMVLLILGSILIFGR"
     gene            complement(468..626)
                     /locus_tag="VN647_08795"
     CDS             complement(468..626)
                     /locus_tag="VN647_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97165.1"
                     /translation="MAIGVVLYKGMEVASLAGVSVSAPHCVMTKTTRRAAWRTEDMKH
                     CGHNGLLG"
     gene            complement(743..1189)
                     /locus_tag="VN647_08800"
     CDS             complement(743..1189)
                     /locus_tag="VN647_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97166.1"
                     /translation="MIGYGKRVLVIGDEEFIDSFLIGELDLHHFVVVAVADGLRVLSE
                     LRRRHFDAVIADMQTPNLNGLALLYQCQLVWPQLPIILMSDHYEDVVDLAINRGAYAY
                     LPKPVEHGKLIRVLSEAITHLVDPGPQRERLSEVFSVYSTRTYSTI"
     gene            1765..2757
                     /locus_tag="VN647_08805"
     CDS             1765..2757
                     /locus_tag="VN647_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWG97167.1"
                     /translation="MTFTPVPFYILCGSLGAGKTTLLMRLLEHWKTQGRRTGVLMNEA
                     GSVSIDGPRAGTLAEQVMNLAGGCVCCDTKEDLSWGIAQLVHDHGSDVLILECSGLAD
                     PAEVIDAVTDAYTARLASLERIMALLHPVSTEESHSSAYVTTQAIRCADELILNKRDL
                     YVPGHWERFRRSILDQNPYARLWETSHARLDTAQLLAPHPSAKHTPPTNVVFDKSRSG
                     SSQRAAYHPIATTIPLPGPLNRRRFLAWITTLPKELERAKGYFRFTQEPELQEFQYAL
                     PGQATIAPLTLLDEPEPALVLIGRGYDVERLRVELLATMEHRDFMSIRTARPLP"
BASE COUNT          694 a          812 c          729 g          634 t
ORIGIN      
        1 cacgtccaaa aatcaaaatc gatcctagga tcagcaacac catgatcgag gccgcattga
       61 tggtcccatt cgcaagcgac gggactccca tcatggcagt cttgccccaa atctgtaccc
      121 cgaagatgtg ccaagcaatc atatgaaatg cgatgtagac gtgcacaagc gccaacgcga
      181 tcatccggta cttgtgacgc ctcgtcatcg gcaggacccc gggagcggaa accttacatg
      241 atgcggctga gtctcgtgga gcgctccgtt tctcaatagc aataagctga aggggaggat
      301 ttatttccat ggtaaccccg gcctcatctg ttactcacat ccactgcctt gaaaatcagc
      361 tcgttcgtat tgactatctg atgcaagaac gtagggaacc tatatacaca ttaaaagaaa
      421 tgaggaatac ccccctgaag agggattgtt aaagaaacat gacgacttca tccaagcaat
      481 ccgttatggc cacagtgctt catatcctcc gtccgccagg cggcgcgccg tgtcgttttc
      541 gtcatcacac aatgaggcgc tgagacgctg acgccggcca gcgacgcaac ttccatcccc
      601 ttgtacaaga caactccgat cgccacagcc accacctttt tctataggaa cctagtccct
      661 ttgtaacgat aaagacctca acggcgttca aagcctagaa cccgccgacg cgtctatccg
      721 cttgattacc agctcaatcg tttcaaattg tcgaatatgt gcgagtgcta tacacagaaa
      781 agacttcgga taaccgttcc cgttgggggc cgggatcgac gaggtgggtg atagcctctg
      841 aaaggacacg gatcagtttt ccatgttcga ctggtttggg caggtatgca taggctcccc
      901 gatttatagc taggtcgacg acatcttcgt aatggtctga cataagaatg atcggcagtt
      961 gcggccacac cagctgacat tggtagagga gggcgagacc attcaaatta ggtgtctgca
     1021 tatcggcgat cacggcatca aaatgacggc gccggagttc gctcaagacc cgcagtccat
     1081 ccgcgaccgc caccaccaca aagtggtgca ggtccagttc gccaatgagg aagctgtcta
     1141 tgaactcttc atcaccgata actaataccc gcttgccata accaatcatc gaatgctcct
     1201 tccgtcagga gtgattcgga gtttccttga tgtgaggccg agacggaaac ccgatacagc
     1261 actcgctcct ccttcgggat tccaaccgga gaagacctta tcaactggtc gagggcatgg
     1321 cattgactag ttctcagcag atcgcagcaa aattgaagcc ttgctgttag ctctacgtag
     1381 tctgtacggt tatatcatgt ggcgagcgag gtgtggcgat cgttaacacc tgcaaggtat
     1441 gtttaaaaag catgatgctg tgcgattctg cagcgagcac cctgtgcgaa agacatcaag
     1501 ggaatagggc tgaagtgctg ggtaacagtc gaccgatggc tagttgaaat gtcgtaagtg
     1561 aaaggtgctg tctgattgtc tacgatccaa gacaggaaaa ctgatcgtac gcatcgggac
     1621 gatcagcaac cgaaagtcta ccgctaatcc cgccgccaga catacacttg aacgctcaat
     1681 cgatgaatct agcggtttct ggaagcaacc cttcatgttc tagacttatc ccggtacaat
     1741 ggggtgcgtt gatcgaggac aagtatgacc ttcactcccg tccccttcta tatcctctgc
     1801 ggctctctcg gcgccggaaa gacgacactg ttgatgcggc tgctggaaca ttggaaaact
     1861 cagggccgac gaaccggcgt actgatgaac gaggccgggt ctgtcagtat tgatggccct
     1921 cgcgccggca cgcttgccga gcaggtcatg aatcttgccg gaggttgtgt ctgttgcgac
     1981 acgaaggaag atctctcctg gggcatcgcg caactggtgc acgatcatgg atcggacgtg
     2041 ctgattctgg aatgctcagg tcttgccgat ccggctgaag tgatcgatgc ggtgactgat
     2101 gcctatacgg cgcgattggc ttctcttgag cgaatcatgg cccttctgca tccggtctcg
     2161 acggaggaaa gccattccag cgcgtatgtg acgacgcaag cgatccgctg tgccgatgaa
     2221 ctcatcttga acaagcgaga cctctacgtc cccggccact gggagagatt ccgccgttcg
     2281 atcctggatc agaaccccta tgcgcgtctg tgggaaacat cacatgcgcg ccttgatacc
     2341 gcccaactct tggccccgca tccatcggca aagcacacgc caccaacgaa tgtcgtattt
     2401 gacaagtcac gatccggctc ttctcaacgt gcggcctacc atcccatcgc cacgacaatc
     2461 ccgttgcctg gtccactgaa tcgccgacga ttcctggctt ggatcaccac cctccccaaa
     2521 gaactcgagc gggcgaaagg ctatttcaga tttacgcagg aaccggagct gcaagaattc
     2581 caatatgcat tgccgggaca ggccactatc gcgcctctta cactgttgga tgaaccggag
     2641 cccgcgctgg tgctgatcgg ccgaggatac gatgtggagc gtctgcgggt tgaactcttg
     2701 gccacgatgg aacaccgaga ctttatgagc attaggaccg cacgtccact tccttgactg
     2761 atctcgcaat cgactccatt tttttcctga acctcggcca tcgaagtaac gcccacgcgg
     2821 cccctccacc cagtgcccct ccgagtatcc acccgccaag cacatcggt
//
LOCUS       DATNDD010000364         4757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3342880, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000364 DATNDD010000000
VERSION     DATNDD010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342880"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1862)
                     /locus_tag="VN647_08810"
     CDS             complement(228..1862)
                     /locus_tag="VN647_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97168.1"
                     /translation="MCFERPNYFCGHMLTDRDLMLQQRYVREKNKLYHRALDGHGIVC
                     GLRITCNHECPGEILIEEGYAIDTCGNDLVVCKTARFDVIQRLREKGLLVEEPKQDPC
                     EPKKPKPKCDVTQCFHVTICYKETEADFTTPFVAGCSPTLTECEPTRIREEVWFDVVQ
                     ELPDELDWIDKLERRIRTCFKLLTHSQFAKKLQEPPTKDLLSKLASGQTMEDRREKCW
                     TLFCELRSLFLLYLRKHPDKYNCTLEEEILHIPFPEDSPNSRTSCHDAICELLKLAWQ
                     HVITCVMGEFVPQCDEPSSASCLVLGTVEIRNGQVVQVCNCPRTYIQQIERMTEILVT
                     SLFSQLACEQRGGEELPARTQNSDAQTSCEKQPHATVCCTDVPFDCRCFVGHILDDPS
                     KLLNNVLAVLRRFRQDRSNFRDLFDFTKTCKTTDSDFAQGLAERKELPAALRQARVID
                     IKLSESVPENIINTLKSPMNRLVDQGQPIYALSHASKDRNEYLSAFQPQGMEAVMQAQ
                     IDSLKAELADLRAQIPSAGSEGPTKGSKKRGSDTQP"
     gene            complement(1985..3388)
                     /locus_tag="VN647_08815"
     CDS             complement(1985..3388)
                     /locus_tag="VN647_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97169.1"
                     /translation="MNNPTKPKPTTCCDEDTTTRYRECIPCEFPPFCRNNYYTGKLLT
                     ERDLTTEQRYLIDKLRLHHLALHGWGIVCGLRIKPHPYCPDKKVILEPGMAIDGCGRE
                     IWLRHEVEICLPKPLQPPPDPEPCPPDPDCVDGKQQDDPANQDSPTTPHPAPEYCPPS
                     PCPPHESFYLCIRYAECETEFSPAPFDECVCDGNPEKPNRICEGYQIIAESYWETPDD
                     WKRILEMHDSCDEDCLAIYPQNAKHCPKPGWPECIPLALIENYTARETITYERIDNDT
                     YRVQLPSVRTLDQVLRCILEKVPSKPLTVIEDIGWTHDHTYHCHEFMKYFAGGEKDCS
                     RSFEITFSGPVLTDGITPYTFQAVVVRHQEKNKGGGFMEVVPATVSYSTDTSGSLSRT
                     KAYLHLNHGFAERYLHNVVFDLYITVRCNCIVDTDGNPVDGNLLARIGNGATVVHTPS
                     GDGIRGGTFESWIHVRP"
     gene            complement(3411..>4757)
                     /locus_tag="VN647_08820"
     CDS             complement(3411..>4757)
                     /locus_tag="VN647_08820"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWG97170.1"
                     /translation="YLIATIRHTVNAGIKQTIHWLRTTEQCATEGELVIGPLDSRIDH
                     CQWHRIEMQAAIPEGTSLQLATATSDTLHTEPPSDPSQKGFIQNSLAPWSIEASAGEQ
                     NPDWLVQNGPGRYLWLRITARSNGQASPVITWIKTHYPRNSYLQYLPAVYQEDEESRN
                     FLDRFLSLFQREFDTLDRKLDNAWQLFEPGSTTDIFSDRSAEQVDQKRSVPFEQRRLQ
                     WLAGWMGLTLNPEWSEVKLREMLRKTAMAYTQRGTVNGLETTITDYSQVGFAKILEHH
                     RIRRWPVLLGREPSSNTARPSTPLDGSMRLWSPNFYQRLQASSNSQIGTFRLTGRPEP
                     AAEPFDWGAHTFSVFFPAHPYTREKTERAVKAAVGREKPAHTKAVLCPVFPRMRVGIQ
                     ATVGVNSVVGAITHVVLGKVATLSYDTILGCSEEERNIRSLKASPRPRVGISTRFP"
BASE COUNT          971 a         1189 c         1333 g         1264 t
ORIGIN      
        1 aaactctttg tactgctcca gctgtaaccc gaagacgatg ccccagcgat ggagggcaag
       61 attcaatcgc cggcgcatct ccatgtgata gcgttgctcg gcttcgaagt cgaagctgcg
      121 cagatactcg cgctcgtaat agtttacgcg ttcgatattc ttagccatag gactctccta
      181 ttcctacatt gtccttgctc gcaacgagat gttcaccgtc gatgtgacta tggttgtgta
      241 tctgagccgc gctttttgga acctttcgtt gggccttcgc tgccagcgct cggaatctga
      301 gctctgagat cggcaagttc tgctttcaga ctgtcaattt gtgcttgcat caccgcttcc
      361 atcccctgag gttgaaatgc tgacagatat tcattgcgat ccttcgacgc atggctcaac
      421 gcatagattg gttgaccttg atcgaccaat ctgttcattg gtgatttaag ggtgttaatg
      481 atgttttccg gtacagattc agagagcttg atgtcgatca cccttgcctg ccgcaatgcg
      541 gctggcaact ctttccgttc cgcgaggcct tgcgcaaaat cggagtcggt tgtcttgcag
      601 gtctttgtga agtcaaagag gtctctaaag ttgcttctgt cttgtctgaa ccgtctcaag
      661 acggccaaga cgttgttcag cagtttcgag gggtcatcaa gtatatgccc cacaaaacat
      721 cggcagtcga agggcacgtc ggtacagcaa actgtcgcat gaggctgttt ctcgcatgaa
      781 gtttgcgcat cgctattctg tgtgcgggct ggaagttctt ctccaccacg ctgctcacag
      841 gcaagctggc tgaacagaga tgtcacgaga atttcagtca ttcgctcgat ctgctgaatg
      901 tacgtcctcg ggcagttaca tacttggacc acctggccgt ttcgtatttc cactgtgccg
      961 agtacaagac aagaggctga ggaaggctca tcgcattggg gcacaaactc tcccatcacg
     1021 caggttataa cgtgttgcca ggcaagcttc agcaattcac agatggcgtc gtggcaggac
     1081 gtgcgactgt taggactatc ctctggaaat ggaatgtgaa ggatctcttc ctcgagtgtg
     1141 caattatatt tgtcgggatg ttttcggaga tacaggagaa atagcgaacg cagttcgcag
     1201 aataaagtcc agcacttttc cctcctatcc tccatcgttt gtcctgacgc aagtttggac
     1261 agcaggtctt ttgtaggtgg ttcctgaagt ttcttcgcaa actggctgtg agtgagtaac
     1321 ttgaaacacg ttctgatgcg ccgctcaagt ttgtcgatcc aatcgagttc gtctggtaat
     1381 tcttggacca cgtcgaacca tacctcttca cgaatacgtg tgggctcgca ttcggttagg
     1441 gtgggagaac agccggccac aaacggagtg gtgaaatccg cttcggtctc tttgtagcaa
     1501 atggtcacat ggaagcattg ggtcacatcg catttcggtt tgggcttctt cggctcgcag
     1561 ggatcctgtt tgggttcttc aactaatagc cctttctctc ttagacgttg aatgacatcg
     1621 aagcgcgctg ttttgcacac caccagatca ttgccacaag tgtcgatggc atacccctct
     1681 tcaatgagga tttctcccgg acattcatga ttgcaggtaa tgcggagacc acagacgatg
     1741 ccatgcccat ccaatgcccg atggtagagt ttgttcttct cgcgtacgta cctctgctgg
     1801 agcatcagat cgcgatcggt cagcatgtgc ccgcaaaaat agttcggacg ttcaaaacac
     1861 atgagctcac agcaggtatc tgtagattga gggtgacatt gacagcccac tggctgcttg
     1921 gccggtgtgt acgttgactg ctgtcgtgaa tggttctgtc ccatggtggg tctcccttat
     1981 cgacttatgg tcgtacgtga atccagcttt caaatgtccc gcctctaatt ccgtctccgc
     2041 ttggtgtatg cacgacggtc gcaccgttgc caattcgagc aagcaggtta ccgtcgactg
     2101 ggttgccgtc cgtatcgacg atacaattgc accggacggt gatatacagg tcgaatacaa
     2161 cgttgtgcag atagcgttcg gcgaatccgt gattcaggtg caagtaagcc ttagtacgtg
     2221 acaagctgcc tgatgtatcg gtgctgtatg agacggtcgc gggcaccact tccatgaagc
     2281 cgccaccttt gtttttttcc tgatgacgta cgacgactgc ttggaacgtg tagggggtga
     2341 taccgtctgt aagcaccggt ccgctgaatg tgatttcgaa ggagcgtgaa cagtctttct
     2401 ctcctccagc gaagtatttc atgaattcat gacaatgata ggtatggtcg tgcgtccatc
     2461 cgatatcttc aatcaccgtg agtggcttgg agggcacttt ctctagaata catcggagta
     2521 cctgatccaa tgtgcggacg cttggaagct ggactcggta ggtatcatta tcgatgcgtt
     2581 catacgtgat ggtttctctc gctgtgtaat tctcgatgag ggcgagcgga atacattcgg
     2641 gccatccagg tttgggacag tgcttggcgt tctgtgggta gatggcgagg cagtcctcgt
     2701 cacaggagtc gtgcatttcg agaatgcgtt tccaatcgtc cggcgtctcc caataggatt
     2761 cggcgatgat ctgataaccc tcacaaatgc ggttcggctt ttcggggttg ccgtcgcaca
     2821 cacattcatc gaagggagcg ggactgaatt ctgtttcaca ctcggcgtat cggatacaga
     2881 gatagaatga ttcgtgcgga ggacatggag atggagggca atactcaggc gcggggtgcg
     2941 gtgtcgttgg actgtcttga ttcgctgggt catcctgttg ctttccgtcg acgcaatccg
     3001 gatctggtgg gcagggttcc gggtcaggcg gtggttgaag cggcttggga agacaaattt
     3061 ctacttcgtg gcgcagccag atttcccgcc cacagccatc gatagccatt cctggttcga
     3121 ggatgacttt cttgtcgggg cagtaggggt gcggtttgat tcgtaacccg cacacgattc
     3181 cccatccatg cagtgccaag tgatggaggc gtagtttgtc gatgagatag cgttgctcgg
     3241 ttgtgaggtc tcgttccgta agcagtttcc ccgtgtagta gttattacgg caaaacggtg
     3301 ggaactcgca ggggatacat tcccgataac gagtggtcgt gtcctcgtcg cagcacgttg
     3361 tcggtttcgg tttggtggga ttattcatgc tgtgctcctg tcgctatgag tcatggaaat
     3421 ctcgtagaga tgccgacgcg tggtcgcggc gatgccttca aggatcgaat atttcgttcc
     3481 tcttcagaac agccaaggat ggtgtcatag ctcaacgtcg ccacttttcc caacacgaca
     3541 tgtgtgatgg cccctacgac gctattgacg cccacggtgg cttgaatacc aacacgcatt
     3601 cttggaaaca ccggacagag cacggctttg gtatgagccg gtttctcgcg accgaccgcc
     3661 gccttgaccg cccgttctgt tttctcgcgc gtgtacgggt gagctggaaa aaagaccgag
     3721 aaggtatgcg cgccccaatc aaatggttct gccgccggct ctggtctgcc ggttaaccgg
     3781 aacgtgccga tctgcgaatt cgacgacgcc tgtagccgct ggtaaaagtt tgggctccat
     3841 aaccgcatcg acccatctaa gggtgtcgag gggcgtgcgg tgttgctgga tggttctcgt
     3901 cccagcagca ccggccaccg gcggatgcga tggtgttcaa gaatcttggc gaatccgacc
     3961 tgtgaatagt cggtaatcgt tgtctcgagg ccgttgacgg ttccccgttg tgtataggcc
     4021 attgccgttt ttctgagcat ctctctgagt ttgacttcgg accattctgg attcagcgtg
     4081 aggcccatcc atccggcaag ccattggagt cttctctgct cgaacggcac cgatctcttt
     4141 tggtccacct gctccgccga tcggtctgaa aagatgtcgg tggtggaacc gggctcgaag
     4201 agttgccatg cgttgtcgag cttccggtcg agggtgtcaa attcgcgctg aaacagtgag
     4261 aggaatcggt ctaagaagtt ccgactttcc tcgtcctcct gatacacggc gggaaggtat
     4321 tgcaggtaag aattgcgcgg atagtgcgtc ttgatccatg tgatcaccgg agaggcctgc
     4381 ccattggacc tcgcggtgat tcgcagccag aggtaccgtc ctggcccgtt ctgaacgagc
     4441 caatccggat tctgttctcc agccgacgct tcgatgctcc atggagcgag cgagttttgt
     4501 atgaatccct tttgagaagg gtcagaagga ggctcggtat gcaaggtatc cgacgtcgcg
     4561 gtggcgagtt gcaagctggt cccttccggg attgcggctt gcatctcgat gcgatgccac
     4621 tggcagtggt cgattcggct gtccaagggg ccgatgacga gttcaccttc tgtggcgcac
     4681 tgctctgttg ttctcaacca atgtatggtc tgtttgatgc cggcgtttac ggtgtgtctg
     4741 atagtggcaa tgagata
//
LOCUS       DATNDD010000365         5170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1293582, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000365 DATNDD010000000
VERSION     DATNDD010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293582"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VN647_08825"
     CDS             <1..1140
                     /locus_tag="VN647_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWG97171.1"
                     /translation="VRANADGPDQANIARGFGAEGIGLCRTEHMFFAEDRIPIMQKMI
                     LARTKEEREKYLDQLLPLQKQDFVGLYREMKGYPVTIRLLDPPLHEFLPKREELMVEI
                     AQLELTGNDGAKLEEKRRLLARVEELHEFNPMLGLRGCRLGITMPEITRMQARAIMEA
                     ACELAKEGAKIVPEIMIPLVGMVSEMKSQKDLIREVAQETMECCDVKLPYLIGTMIEL
                     PRAAVTAEHIAKEAEFFSFGTNDLTQTTFGFSRDDAAKFIDHYRTVGIMESDPFAVLD
                     REGVGSLMKQAVAGGRRTRSDIKLGICGEHGGDPNSIEFCHELGLDYVSCSPYRVAIA
                     RLAAAHAALSEAEAQKSIKTATSSRVKPPRSQPRRRRSRVTRKAR"
     gene            1137..2255
                     /gene="ribD"
                     /locus_tag="VN647_08830"
     CDS             1137..2255
                     /gene="ribD"
                     /locus_tag="VN647_08830"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWG97172.1"
                     /translation="MSGHAQDLHYMTLALRLAAKGRETTSPNPMVGAVVVGQGRIIGQ
                     GFHLRPGSPHAEILALRQAGNRARGGTLYVTLEPCCHLKKRTPPCVPEILRSRIRRVV
                     IAMQDPNPSVQGKGAAALRRAGLSVTVGVARSEAEALNKSYCHWMKTGRPYVTLKAGM
                     TLDGKLATATGESRWITGKASRREVHQLRRCVDGVLIGVDTVLTDDPSLTARTGPRLE
                     KLALQQPLRIVVDSRLRIPLKAQVLTQQDKAKTIIATTAAAPTARRSVLQKQGIEIFV
                     VPALQGRVSLPALLKQLGRRGITSLLVEGGSEVNAAMLKAKLIDHIRLYMAPLLLGGR
                     NAKGVIGGESPARLAGAIEIQHVAIRFVGDDVVVEGDL"
     gene            2252..3538
                     /locus_tag="VN647_08835"
     CDS             2252..3538
                     /locus_tag="VN647_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97173.1"
                     /translation="MTGWIRFISLTLVLSSGMNSSVAEESVPVIENLTSLVLQYIDAH
                     GDEAGRLLQRILLDKRASVETVSQIIKAERTYQSQSVGNLPDEQIVIRGQPYPLSLFV
                     PPTYQTSRAYALVVCLHGFGFTGEEYLERWRPRLGEDYLLACPSYPSGAWFTRRAEEL
                     VLETIRQVAHRYHIDPDRVFLTGMSNGGIGTWLIGMHHASLFAGLAPMASGLDDVLMP
                     FLANLRSTPIYIIHGAKDQVMPVDLSRSIVRELDKLGYPYVYREHHREHPMAGGHYFP
                     REELPDLVAWFNRQRREPLPTSLTVVRDGSHFQPFNWIRLDATDPIAAFSDDLVDKRD
                     ERIKRRVYARLDASIAGSNRIEVKAEHVQRYSLFLNEQLINVSKPLTVVTNGRLSFEG
                     TVTPTVETLLRQARLRQDPERLFSVHLIIDITKQAP"
     gene            3535..4350
                     /locus_tag="VN647_08840"
     CDS             3535..4350
                     /locus_tag="VN647_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97174.1"
                     /translation="MISAWRFRSGPVAHMLVVWIAMGIVVFAGDARSETCTLSPVHAG
                     LTFPIERVDPVRICRLQAIIQSYTTVSKVGPIRTALSESMYRHLLDHPPFTADVIRRL
                     HLGLYRSEARGPGRFWGDDGEGTKGIVELVYEDPTFRIYLLEGTHESRLLPHVTGKAV
                     VFLRIGVMRDANSHEATDSTLVAYTKLDNRFLSGLVSLLHPLVSRVVTSRLQKGVETV
                     DRLGVVMRLDSQRVMSVLTNPPPLPEHHLAFLKQTLIGRQSTDKGSSDGRSIP"
     gene            4347..>5170
                     /locus_tag="VN647_08845"
     CDS             4347..>5170
                     /locus_tag="VN647_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG97175.1"
                     /translation="MTTSRSFTLICTVGIFCFISYNMVRMPALSLFAESLGASPERIG
                     LIVSVSTLTGVLLKLPSGALSDIYGRRFLLRIGVVAFGLPPFLYPFITNLDALTALRF
                     LHGFATAIFAPSALATVAELYRERRGAALGTYTACTQSGSLLGPFLGGYLVHAAGFPT
                     AFVTAGAFGCIAMVLFYSLHLDISVPQRKEQGTAVVLSEMWKGFAAVAKNSKVLITSM
                     TDAAKMIANGALMAFLPLYGVAVGLNPGEVGLLFTVQAFTSFFSKPIMGRISDRVGR"
BASE COUNT         1069 a         1385 c         1554 g         1162 t
ORIGIN      
        1 gtccgtgcca acgccgatgg gccggaccag gcaaatatcg ccaggggatt cggcgcggag
       61 ggcatcggtc tctgccgcac cgaacacatg ttcttcgccg aagatcgtat cccgatcatg
      121 cagaagatga tcttggcccg aacgaaagag gagcgtgaaa aatacctgga tcagttgttg
      181 ccgttacaga aacaagactt tgtcgggctc tatcgagaga tgaagggcta tcctgtgacg
      241 attcgcttgc ttgacccgcc gctccatgag tttctgccca agcgggaaga gctgatggtc
      301 gagatcgcgc agctggaatt gacgggtaat gacggtgcca agctggaaga aaaacgacgg
      361 ctcctcgcgc gagttgagga actgcatgag tttaatccga tgctcgggct tcgtggctgc
      421 cggttgggga ttaccatgcc ggagatcacg cgcatgcagg cgagggcgat catggaggct
      481 gcctgcgaat tggcgaaaga gggtgcgaag atcgtacctg aaatcatgat cccactcgtg
      541 ggcatggtct ccgagatgaa gtcgcagaaa gacctgatcc gtgaagtggc tcaagagacg
      601 atggaatgct gcgacgtcaa actgccgtat ctgatcggga cgatgatcga attgcctcgc
      661 gcagccgtga ctgccgaaca tatcgccaag gaagcagaat tcttttcttt cgggaccaat
      721 gatttgacgc aaacgacatt tgggttttca cgcgatgatg cggccaagtt catcgatcat
      781 tatcgaaccg tcggcatcat ggaatccgat ccgtttgccg tattggacag ggaaggtgtc
      841 gggtcgctga tgaaacaggc ggttgcggga ggccgtagga cgcgatccga tatcaagctg
      901 ggcatatgcg gtgagcatgg aggtgatcca aactctatag agttctgtca tgagctgggc
      961 ttggattatg tgagctgttc gccgtaccga gtggcgattg ccagattggc agcggcacat
     1021 gccgctctgt cggaggcaga ggcccagaag tccattaaaa ccgctacttc gtcacgagtg
     1081 aagccgccga ggtcacagcc acgtcgccgt cggtcccgcg tgacccgcaa ggcacggtga
     1141 gtggtcacgc tcaggacttg cactacatga ccctggccct tcgcctggcg gcgaagggcc
     1201 gggaaacgac aagcccgaat ccgatggtcg gagcggtagt ggttggtcaa ggcaggatca
     1261 tcgggcaggg ttttcacctc cgacccggga gtcctcacgc agaaattctc gcattgcgcc
     1321 aggcagggaa tcgcgcccga ggtgggactc tctacgtcac gcttgagcct tgctgtcatc
     1381 tgaagaaacg cactccccct tgcgtcccgg aaattcttcg ctcacgcatc cgccgtgtgg
     1441 tgattgcgat gcaggatccg aatccatcag tgcagggaaa aggagccgca gcgcttcggc
     1501 gggcaggact ctcggtcacg gttggggttg cccgctctga agcggaagca ctgaacaagt
     1561 cgtattgcca ttggatgaaa accggccgcc cctatgtgac gctcaaggcc ggcatgacgc
     1621 ttgatggaaa gctggccacg gcaacagggg agtctcgatg gattacgggc aaggcgtctc
     1681 gtcgagaggt gcatcagctt cgccgttgtg tcgatggcgt cttgatcgga gtagacaccg
     1741 ttctgactga tgatccgtca ctgacagcga gaacagggcc gcgactggaa aaattggcat
     1801 tgcagcagcc tcttcgcatt gtcgtcgata gccgactgcg tataccgctc aaggcacagg
     1861 tcctgacaca acaggacaag gccaaaacga ttatcgccac cactgctgca gccccaacgg
     1921 ctcgacgatc ggttctgcaa aagcaaggca tagagatctt cgtggtgcct gccctgcagg
     1981 gtcgagtttc gttgcccgct ctgctcaagc aactcggtcg gcgcggtatc acatccttgc
     2041 tcgtggaggg gggtagtgag gtcaatgcag cgatgctgaa ggcaaaattg atcgatcaca
     2101 ttcgccttta tatggcgccg ctgcttctcg gcggacggaa tgccaagggt gtgatcgggg
     2161 gagaaagtcc ggcgcggctc gcaggtgcca ttgagataca gcatgtggcg atacggttcg
     2221 tcggcgatga tgttgtggtg gaaggggatc tgtgaccggc tggataagat tcatcagctt
     2281 gacgttggtc cttagtagtg gaatgaattc cagcgttgca gaggaatcgg taccggtcat
     2341 cgagaatctg acttcgctcg tcctccagta catagacgcc catggtgatg aagccggtcg
     2401 attgttgcag cgcattctcc tcgataaacg cgcatctgtc gaaacagtct ctcagatcat
     2461 aaaggctgaa cgaacgtatc agagccaatc agtcgggaat cttcccgacg aacagatcgt
     2521 catccgggga cagccgtacc cgctgtccct gttcgtcccg ccgacgtacc aaacttcgag
     2581 ggcctatgcc ttagtcgtgt gtctgcacgg attcggtttt accggcgagg aatatttgga
     2641 gcgatggcga ccacggttgg gtgaggacta tctattggcc tgcccctcct atccctccgg
     2701 tgcgtggttt acaagacgtg cggaggagct ggtgctggag acgatccggc aagttgcgca
     2761 tcggtatcat atcgaccccg atcgggtttt cctcacgggc atgtcaaatg gcgggatcgg
     2821 tacctggctg atcggtatgc atcatgcgtc attgtttgcc ggcctcgcac ccatggcgag
     2881 cggactcgat gacgtgctga tgccgttttt agcgaatctt cgaagcacac cgatctatat
     2941 cattcatgga gccaaagatc aggtgatgcc ggtggacctg agccgctcga tcgttcgaga
     3001 gctggataag ctcggctatc catatgtgta tcgcgagcat caccgtgagc atcccatggc
     3061 aggcgggcac tatttcccca gagaagagtt gccggacctt gttgcctggt tcaatcgtca
     3121 gcgccgtgaa cccttgccga caagcctgac cgttgtgcgt gacgggagcc actttcaacc
     3181 gttcaactgg atccgcctcg atgccactga tccgatcgcg gcattttcag acgatttggt
     3241 ggacaaacgc gacgaacgaa tcaagcgtcg ggtgtatgcc aggcttgacg cgtcgattgc
     3301 cggaagcaat cgcatcgagg tgaaggccga gcacgttcag cgctacagcc tattcctgaa
     3361 cgagcaattg atcaatgtct ccaaaccgct cacggtggtg acgaacggcc gtctctcgtt
     3421 cgaaggaacc gtcactccaa cagtggaaac cctattgcgc caagctagac tgcgacaaga
     3481 ccccgagcgc ctcttttccg ttcatctcat aattgacatc acgaagcagg ctccatgatc
     3541 tccgcatggc ggttccggtc tggtcctgtg gcgcacatgc ttgttgtgtg gatcgctatg
     3601 gggattgttg tatttgccgg ggatgcccgg tcagaaacat gtacgttgtc ccccgtccat
     3661 gccggactca catttcccat cgagcgagtg gatcctgtcc ggatctgtag actgcaagcc
     3721 atcattcaaa gttatacgac tgtgagcaag gtgggaccga tccgaaccgc tctctctgaa
     3781 tcgatgtatc ggcatcttct ggatcatccg ccctttaccg cggatgtgat ccggcgtctg
     3841 catcttgggc tttaccggtc ggaagcgcga ggtccgggcc gtttctgggg cgatgacggc
     3901 gaggggacga agggcatcgt tgagctggtg tatgaggatc ccacattccg catctactta
     3961 cttgagggaa ctcatgaaag ccggttgctt cctcacgtga cgggaaaagc ggtcgtcttt
     4021 ctcaggatag gcgttatgcg agacgcgaac agccatgaag cgacggacag tacactggtt
     4081 gcgtatacga agttggataa tcgttttctt tcagggttgg tttccctcct gcatccgctg
     4141 gtttccaggg tcgtgacaag tcggttgcaa aaaggagtgg agactgtcga tcgattgggg
     4201 gtggtgatga ggctggactc ccaacgggtg atgtctgtat tgacgaatcc gccgccttta
     4261 ccggaacatc acctggcgtt tctgaaacag acgctaatag gccgacagag caccgacaag
     4321 ggtagctcgg acggcagatc gatcccatga cgacatcgcg cagcttcacg ctgatctgca
     4381 cggtcggcat cttttgcttc atcagctaca acatggtgcg gatgcccgcg ctctccttgt
     4441 tcgcggaatc gctcggcgca agcccggagc ggatcggtct gatcgtgtcc gtttccacct
     4501 taaccggcgt cttgctgaaa ctgccctccg gcgccctctc cgatatctat ggaaggcggt
     4561 tcctgctgcg gatcggtgtg gtggcgttcg ggctgccgcc atttctctat cccttcatta
     4621 cgaacttgga cgcactgacg gcgctacggt ttcttcacgg cttcgcaact gctattttcg
     4681 ccccgagcgc tcttgctacc gtggcggagc tgtatcggga acggcgtggt gcggcgctcg
     4741 ggacctatac agcctgcacg caatccggct ctctcttggg cccttttctc ggaggatacc
     4801 tcgtccacgc ggcagggttt cccacggcgt ttgtcacggc cggcgccttc ggttgcatcg
     4861 ccatggtgtt gttctatagc cttcatctcg acatctcggt gccgcagagg aaggaacagg
     4921 ggacggctgt tgtgctgtcc gaaatgtgga aagggtttgc cgccgtcgcc aaaaacagca
     4981 aggtgctgat caccagtatg accgatgccg ccaagatgat cgcgaacgga gccttgatgg
     5041 cgtttttgcc gttgtacggg gttgcggtag gtttgaatcc cggcgaagtt ggtcttttgt
     5101 ttacggtcca ggcatttacg tcgttcttct ccaagccgat tatggggcgg atatcagacc
     5161 gagtcggtcg
//
LOCUS       DATNDD010000366         6365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1156169, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000366 DATNDD010000000
VERSION     DATNDD010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156169"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VN647_08850"
     CDS             complement(<1..542)
                     /locus_tag="VN647_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97176.1"
                     /translation="MKAIWGILLAALFLALPARADTLYTISGTASIHGNDACVGPCLE
                     TVDFSFNVTESLDPTYKEYFLTLTGASVTAQGSLGPFSKAPGITLDNGWYAPIYLGGS
                     DLIFDSELDINFSGNFEARPFVPDVIAATLYSCGDQTCLTDFCPTDWVMCQETMFPIK
                     GLWINANLTNVSVVDPVMTP"
     gene            complement(547..1434)
                     /locus_tag="VN647_08855"
     CDS             complement(547..1434)
                     /locus_tag="VN647_08855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG97177.1"
                     /translation="MAKGIKGLQGINLCSKGEKMWLHRLRVRTFNALQSVGIHAIPNH
                     FYGPIPDTRLPEETWTGRSSLPGIRMNEPCQIELAQMLCGQFGEEFAAIPTGTQFGGV
                     DGEVLHGMVRYFRPSRILEVGSGTSTRISAAAVVQNGCGEVVAVEPYPNDTLKAGFPG
                     LSRLVVSPAQSVPMAEFESLGEGDILFIDSSHVLKIGSDVQFLFLEVLPRLKPGVIVH
                     VHDIFLPQSYPRKWVVDELRFWNEQYLLQAFLAFNSAFEVLWAAHYMNLRHPEVMGLF
                     PSYQSGVSFPASFWMQKKR"
     gene            complement(1746..2855)
                     /locus_tag="VN647_08860"
     CDS             complement(1746..2855)
                     /locus_tag="VN647_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWG97178.1"
                     /translation="MAEHDCDDRDDRDDCEYLVDSILPTQEMHLLGGPSGSGKSTFLF
                     KFLQSWSRGEPQWNYPSHPVPYLYVSLDRSSKGVVRTLRRMGIDISTIPYFAGFDDPT
                     LVTIDDVLGAALRFKPDARLFVVEGIAGKVPKNKINDYGEVAAFVSHIGRWCRQFNAT
                     VIGTMHAAKTKANEQYDNPRQRILGSVAWGAFTETIIGLEPIDAKDPECRLRRLYLLP
                     RNAPEQTFDMEWQGGHLVPATADTMVKQRRPKRGRPDNALKEALEGIQRYLKDQQIKR
                     ITFGELLNASPLPPATLNRGVRTLIESGFLKVLKKGYYEVMLFDDPLVADESSLPPQP
                     KCFDSTITEAEDENDSPEGSEIEPEADEGPHTGEF"
     gene            complement(3052..3384)
                     /locus_tag="VN647_08865"
     CDS             complement(3052..3384)
                     /locus_tag="VN647_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97179.1"
                     /translation="MSVRTIKRSTIPNPIRGSGGLKSALHSLPEWSKIEKVIEKGLTP
                     AEAVEITIDAPPSVLRLKNPIGSLVHLLRKEVKKSKEPLLVSVRKDPTAPGPYKARVY
                     LVTVANPV"
     gene            complement(4244..4630)
                     /locus_tag="VN647_08870"
     CDS             complement(4244..4630)
                     /locus_tag="VN647_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97180.1"
                     /translation="MGDDNDSLVPPLSYLLECSETSLRNFLVVNLNKSANIRKKIRGL
                     LEERAECEGLAFIAEWLIEYGPQLLALSGAESLEKEKEQEQNREEKREVIELENSLTE
                     RNERIQRQLTLADWRSMTRKWRKRAG"
     gene            5139..5462
                     /locus_tag="VN647_08875"
     CDS             5139..5462
                     /locus_tag="VN647_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97181.1"
                     /translation="MNKNAKAWVRALRSGKYQQGKGRLRKGDSYCCLGVACELAKRKR
                     VIKRFVGNRSNLPQKVKKWLGLRSEEGAYDFGYLTTDNDIWDKTFAQIADIIDSEPEG
                     LFVEE"
     gene            5583..5969
                     /locus_tag="VN647_08880"
     CDS             5583..5969
                     /locus_tag="VN647_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97182.1"
                     /translation="METDGGWGGGWREGVCIVDKFWEVYLTLVKAIVIAAIWSVQLPL
                     MGGYLGIWAVIGGLNWMREKVEEWKPMRPDVFRIPLDDKAEVIWCIQARKDIHDLLAH
                     VLNFEPLLRADQINMIEERYRSGSGE"
     gene            6183..>6365
                     /locus_tag="VN647_08885"
     CDS             6183..>6365
                     /locus_tag="VN647_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97183.1"
                     /translation="MTPKPESGSTLEWQPALIFDAHADQRPWSLHPDVQALLGKVIRV
                     REDGSGEYVPEECKTFC"
BASE COUNT         1540 a         1498 c         1912 g         1415 t
ORIGIN      
        1 tcgggcgtca ttacgggatc gaccacggag acattggtca ggttggcgtt gatccacaga
       61 cctttgatcg ggaacatagt ctcttgacac ataacccaat ccgtagggca gaaatcggtg
      121 aggcaggttt ggtcgccgca agaatagagg gtcgcggcga tgacatcagg cacgaagggc
      181 cgagcttcaa agttccctga gaaattgatg tccaactcac tgtcaaatat gagatcactt
      241 cctcctaggt aaatcggagc gtaccaacca ttatccaaag tgattccagg cgccttgcta
      301 aacggtccaa gggatccctg cgcggtcacg gacgcgccag tcagcgtcag gaagtactct
      361 ttgtaggtgg ggtctaggga ttccgtgacg ttaaacgaga aatcgaccgt ttctaggcac
      421 gggccgacgc aggcatcgtt cccatgtatg ctggctgtcc cgctgatggt gtagagtgtg
      481 tccgcgcgcg ccgggagcgc cagaaataga gccgctagaa ggattcccca gatggctttc
      541 atggtttcac ctcttctttt gcatccaaaa gctggccgga aacgatacgc cggattggta
      601 ggacgggaac agacccataa cttccgggtg cctgagattc atgtagtgcg cggcccacag
      661 cacctcgaac gcggaattga acgccaggaa cgcctgcaga agatattgct cattccagaa
      721 gcgcagttcg tcaactaccc atttgcgcgg atagctctgc ggcaagaaaa tatcgtgaac
      781 atggacgatg acgcccggct tcaagcgcgg caatacctcc aggaacaaga attgcacgtc
      841 actgccgatc ttaagcacgt ggctgctatc aataaacagt atgtcgcctt cgcctagaga
      901 ttcaaactcg gccatcggga cgctctgtgc cggcgatacc actaagcggc ttaggccagg
      961 gaatccggct ttgagagtgt cattcggata cggctcgacg gcgacgacct cgccgcagcc
     1021 attctggact accgcagccg cagaaatgcg cgtggacgtg cctgagccta cttccaggat
     1081 ccgcgaggga cggaaatacc ggaccatgcc gtgcaagact tcgccgtcca cgccgccgaa
     1141 ttgagtacca gtggggatag cggcgaactc ctcgccaaac tgtccgcata gcatctgagc
     1201 tagctcgatt tggcacggct cgttcatgcg gatgcccgga agggacgatc ttccagtcca
     1261 tgtctcctcg ggcaaccgcg tatcgggaat cggcccgtag aagtgattcg ggatggcatg
     1321 gatgcccacc gactgaaggg cattgaaagt acgcacgcgc agcctatgaa gccacatctt
     1381 ttcccctttg ctgcaaagat ttatcccctg aaggccctta atccctttcg ccacggattc
     1441 gccacggatt cacctgcacg atcttttccc ttccatccga aataaggtta tatggataat
     1501 tctcaatgag cctctatcaa cctctgtcga tttcgcctga gtttcgcctg cgcgggagac
     1561 gccgaaaggg ccaaaataga gagataaagt actagaacta tcctttctga gcccgtgggg
     1621 accacacact ggggaatttt gattgattct cctctccacc ccccttatat atatgatata
     1681 tatatagata attcaatatt taggatcaat caaaattcaa aaaggagacc aaagataaca
     1741 atcaatcaaa attccccagt gtgtggtccc tcgtcggctt ctggctctat ttctgaccct
     1801 tccggcgagt cattttcgtc ttctgcctcg gtaatagtac tgtcaaaaca cttaggttgt
     1861 gggggtagcg atgattcatc ggctacgaga ggatcatcaa atagcatcac ttcgtaatag
     1921 cccttcttga ggaccttgag gaagccggat tcgatgagag ttctaacgcc gcggttgagt
     1981 gtggccgggg ggagaggaga ggcgttgagg agttcgccga aggtgatgcg tttgatttgt
     2041 tgatccttca ggtagcgttg gattccttcg agggcttctt tgagtgcgtt gtcggggcgt
     2101 ccgcgcttcg gccggcgctg cttgaccata gtgtcggctg tcgccggaac aagatgacct
     2161 ccctgccatt ccatgtcgaa ggtttgctcg ggcgcgttgc gagggaggag atagagacgg
     2221 cgtaagcggc actcgggatc tttcgcgtcg atcggttcaa gtccaataat cgtttcggtg
     2281 aaagctcccc aggcaaccga tccaaggata cgctggcggg gattatcgta ttgctcgttt
     2341 gcttttgttt tagcggcgtg catggtgccg atgacggtgg cgttgaactg gcggcaccat
     2401 cggccgatgt gagagacgaa ggcggcgact tctccatagt cgttgatttt atttttgggg
     2461 actttaccgg cgatcccctc gacgacaaag aggcgcgcgt cgggtttgaa gcggagagcg
     2521 gcgccgagta cgtcatcaat cgtaacgaga gtggggtcat cgaaaccggc gaagtagggg
     2581 attgttgaga tgtcaatacc cattcgacgg agggtgcgga cgactccttt ggatgaacgg
     2641 tcgagggata cgtagaggta agggacggga tgggaggggt aattccactg gggctcgccc
     2701 cgggaccaag attgcaggaa tttgaaaagg aaggttgatt tgccggagcc cgatggaccg
     2761 cctaaaaggt gcatttcttg tgtggggaga atcgaatcaa cgagatattc gcaatcatcg
     2821 cgatcatcgc gatcatcgca atcgtgttca gccaaagttt cctctccaaa agggattgaa
     2881 cttttttatc gaaagcgagg aagctcgtag cggttctcac cggagcacgc aaacgattag
     2941 aaggggagag tagcagggga gagtgggagg ggtcaactga agagaaacga aaagggagac
     3001 ctaagagtta acctttctct cccggagtct cccttttttg aatcgacgca atcacaccgg
     3061 attcgcaaca gtcacaaggt agacgcgggc tttatagggg cccggcgccg tgggatcctt
     3121 ccgcactgac acgagaagag gctctttcga cttcttcacc tctttgcgaa gaagatgtac
     3181 gagagagccg atagggttct tcagcctgag cacagaaggc ggagcgtcga tagtgatctc
     3241 gaccgcttca gccggcgtga gtcccttttc gattactttc tcaatcttcg accactcagg
     3301 aagcgagtga agagcggatt tgagcccgcc tgatccacgg atcggattcg ggatagttga
     3361 ccttttgatt gttcttactg acatgtgagg tagtgctcct ttcaaagttt tctttgtttg
     3421 tcgtcgcccc attgggaact gatgtagtga cttaatacgt agaatacatt acagatgtcg
     3481 atgatgcaag ggaaatacct agatcactgt atttttacgg aggagcgaga gaggggatga
     3541 ggaggcgatg aggaggagat gagggcggga tgaggaggag agagggcagg ttctatctaa
     3601 cccgccctct cgatcaggcg agttgaggag gaactcgccg acctatcggg aggaacgata
     3661 ggtatcgtgg cgcgtcatcg tggacgcgat ttatggagag aaggataaag gaaaggagaa
     3721 ggggaaatca agtttcgggg agcggggcga tccaggccta tctatccatc tcgcggcgga
     3781 attaacaacc ttaacctttt agacaacgat taagaaggtg aaagattgcg cttgacaccg
     3841 gccctcatca tatctaaagt ggcgatatga aatatctcca ttgtttgcga tgtgggtata
     3901 actggctgcc gcgaattttg aagccgatgc gatgcccgcg gtgcaactca tggctgtgga
     3961 ataagccgcg gaagaaggac caatcgccat cggtgcgggc ggacggtcaa gaggctgcgc
     4021 gagcggccgc gcggacgact tcggcgccgg gtaaaccctc aacggaagaa gcggcctaga
     4081 gcgacatgga tagagccatg cggaatactc atctcgtgcg cccgccaatg caacatagta
     4141 agaactcgcg cattatcaac gacatagagg agagaagata cactagtagc gtccttgtcg
     4201 acaagggatt atttatcgcc gacgacgccg acgactatcc agtctatccg gctcgtttgc
     4261 gccacttgcg cgtcatgctg cgccagtcgg cgagcgtgag ctgccgctgg atccgctcgt
     4321 tccgctcggt caatgagttc tcaagttcaa tcacctccct cttctcttcc ctgttctgtt
     4381 cctgctcctt ctccttctct agcgattcgg ctcctgagag cgcgagcaat tgcggcccgt
     4441 attcgatgag ccattcagcg atgaacgcga gtccttcaca ctccgcacgt tcttcgagga
     4501 gtccacgaat tttctttctg atgttcgcgg acttgttcaa gttgacaacg aggaaattac
     4561 ggagcgatgt ttccgagcac tcaagcaagt atgaaagcgg tgggactaaa ctgtcgttgt
     4621 cgtcgcccat ccgagttcct ttctagatac gtagagaggt tgccatccca agcacaggag
     4681 tgcgggatag aggaggagat ttgcttgcaa aaccgagcaa agggtaatgg gagtaatggg
     4741 aatgggactg acaacgaggc ggacgacggg actgacgacg ggctctgagg gggaatagca
     4801 ggggtgcgga ttgagcgaca agttgaacgc gcgaatattg ctgaggctcg acgaccaaac
     4861 ctcgcgggag gtgcggatct tcgcggactc aacagagaga acgttgcacg gtgcactgag
     4921 atttttgctc aagcggggat tggaacacga aagccggggc gagggtggta agaagcgtcc
     4981 gatttcgtca agttcaggta acaggaaggc agtgggagga gcggcatgaa ggcagatgat
     5041 tttcaggtgc cggatcgttt cgcttggcgc gggtatctgt atgcgctggc gttcatcttg
     5101 gcgctcgggg ctgcggctta tttcattgtg agggtgctat gaacaagaat gcgaaggcgt
     5161 gggtaagggc gctgcgaagc gggaagtatc aacaggggaa gggccggctt cgtaagggcg
     5221 atagctattg ctgtctcggt gtggcgtgcg agttggcaaa aagaaagcgc gtgattaaaa
     5281 ggttcgtcgg caaccgatcg aatcttcccc aaaaagtcaa aaaatggctc ggactgcgtt
     5341 ctgaggaagg cgcatacgac ttcggttatc taacgaccga caacgatatt tgggacaaaa
     5401 ctttcgcaca aattgccgac atcatcgact ccgagcccga aggacttttc gtagaggaat
     5461 aacagtaacg tagcggggat aagggatggg gatgggatgg ggatgggatg tgactcacaa
     5521 aatagggggg aacaatggga tcatcggcgg gagcgggatc aacagaatca atgatagggc
     5581 cagtggaaac agatggtgga tggggagggg gatggagaga aggcgtctgt atagttgata
     5641 agttttggga ggtatatctc actctcgtga aggcaatcgt gatcgccgcg atatggagcg
     5701 ttcagcttcc gctgatgggg ggatatttgg ggatctgggc ggtgatcggg ggactgaatt
     5761 ggatgagaga gaaggtggag gagtggaagc cgatgaggcc ggatgtgttt agaataccgt
     5821 tggacgacaa agcagaggtc atatggtgca tccaggcgcg aaaagacatc catgatctgc
     5881 ttgctcacgt tcttaatttc gagcccttgc tgagagccga ccagatcaat atgatcgaag
     5941 aacgatatcg gagcggatca ggtgagtgag ataatggctc cctcctcgcc caagcccgag
     6001 agactgacgc gaacgcaacg tgaattgagt gtattcgagc cggagttttg ccctaactgt
     6061 ggccgaggat tcgatcacca atacaagggc gtgaagaaac tgacgcctgg gcatgatgag
     6121 caatgccgct ggtctacctt ctgcggatac tgcggagcga tttacactgt ggagattgag
     6181 ccatgactcc caagcccgag agtggaagca cgctagagtg gcaaccggcg ttgatctttg
     6241 acgcgcacgc cgatcagaga ccttggagtt tacatcccga tgtccaggct ttattaggca
     6301 aggtcataag agtgcgtgaa gatggcagcg gcgaatatgt gcccgaggaa tgtaagacat
     6361 tttgc
//
LOCUS       DATNDD010000367         3489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1297737, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000367 DATNDD010000000
VERSION     DATNDD010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297737"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="VN647_08890"
     CDS             <1..1778
                     /locus_tag="VN647_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWG97184.1"
                     /translation="LAEQLGAETVMLSGENVAEEILSYARSRNATKILVGKPVRPVWK
                     EWIFGSVVSDLVHRSGEIDIFVIAGEAGEGQPLVRRNLRRTSDVRAYGYALIGVLCAT
                     AVAWLMFPYFATANLIMMYLIAVVVVAIRFGRGPSALASILSVAAFDFFFVPPYFSFA
                     VTDIEYLLTFGVMLVVALIIGNLAVRIRQQAELSRDREHRTGVLYSMSRDLATHRGTG
                     MLAQLATKHLRDVFDAQVAVFLADADKKVQLQRGEPLFFELEPKESGVAQWVYDHNER
                     AGLGTDTLPGASALYVPLVCSTGPIGVIALRPKNAALLVDPEQLHLLESLMNQVALAI
                     ERACLSDEAQQAHVRAETERMRNAILSSVSHDLRTPLATITGAASSLAAERGEFDPVA
                     RRELAQSIYREAERLDRLLKNLLDMMRIEAGVVQLKKEWHSVDEVAGAVLAGLDGRLH
                     DHAVNTSFPADLPLVLVDGVLLEQVVINLIENAVKYAPARTMIELAASATDREIVVEV
                     ADRGPGIPVGEESHIFDKFYRAKPAREGGVGLGLTICRGIVEAHGGRIWAENRSGGGA
                     LFRFSIPLPNQQPPIETEQPEARDA"
     gene            1806..2498
                     /locus_tag="VN647_08895"
     CDS             1806..2498
                     /locus_tag="VN647_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97185.1"
                     /translation="MSQDATILLIEDEPEIRRFLRTILPAHGFRLYEAATGQDGLTET
                     KARNPDLILLDLGLPDLDGGEVIRQVREWTATPIIVLSARDQEQAKVAALDLGADDYI
                     TKPFGVNELLARVRTAFRHAARTGDGGESVFVLGDLKVDLGRRQVFVSGKEVHLTPIE
                     YKLLTTLIRYAGKVLTHRQLLKEVWGPLHVDEGHYLRVYMRQLRNKLERNPVHPRYLM
                     TELGVGYRLRTE"
     gene            2624..>3489
                     /locus_tag="VN647_08900"
     CDS             2624..>3489
                     /locus_tag="VN647_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWG97186.1"
                     /translation="MRQAGAAYSFMDNAKAVNHEYGWRKHISLLLIAWGLFTTVPICV
                     AQTQDSNQALKETMAPIPGANRPTDAATTDWHYGAYVDVGYIGNFNFPDNHLWRSRTT
                     ANHDNELSPNMGLAYVRKDASVSSRWGMELGFQGGRDTEEFAFLVGEKRVDGADVLRH
                     VHRANVSYLAPVGKGLTFTAGLFNSLMGYESLYARDNANYTRSWIADNTPYMMFGVNA
                     QYPVNDNLTVTAFVVNSYYHLAHPNDLPSYGGRWAWKVTPRLTFIQTLYGGPDQTDTS
                     LEFWRVYGNHIL"
BASE COUNT          745 a          901 c         1048 g          795 t
ORIGIN      
        1 ggctggcgga gcagcttgga gccgaaacgg tcatgttaag cggcgaaaac gtcgccgaag
       61 agattttgag ctatgcgcgg agtcgcaacg ccacaaagat tcttgtcggg aaaccggtac
      121 gtccggtttg gaaggagtgg atctttggct ccgtggtgtc cgacctcgtt caccggagcg
      181 gcgagatcga tatctttgtc attgccggag aagcgggcga aggtcagcca ctcgtccgac
      241 gcaatctccg aagaaccagt gatgtccggg catatggcta tgccttgatt ggggtgctgt
      301 gtgcgacagc ggtcgcctgg ctgatgttcc cctatttcgc gaccgcgaac ctgatcatga
      361 tgtacttaat cgccgtcgtc gtggtcgcga ttcgtttcgg tcgcggtccg tcggcgctgg
      421 catcgatctt gagcgtggct gcattcgact tttttttcgt gcctccctac ttctccttcg
      481 ccgtcaccga catcgaatat ctgttgacct tcggggtgat gcttgtggtc gcactgatca
      541 tcggcaacct ggcggtacga attcggcaac aagctgaact gtctcgtgat agagagcatc
      601 gcacgggtgt gttgtattcc atgagccgcg atctcgccac tcatcgagga acggggatgt
      661 tggcccaact cgcaaccaag catcttcggg acgtgtttga cgcgcaggtt gctgtattcc
      721 tcgcggatgc cgataaaaag gtacagctgc aacgggggga acctctgttt tttgaattgg
      781 agcctaagga gtcgggtgtt gctcaatggg tgtacgacca caatgaacgg gccggcctcg
      841 ggaccgatac gcttccgggg gccagcgcgc tgtatgtgcc gttggtatgc tcaacggggc
      901 cgatcggcgt catcgctctc cggccgaaga acgccgcgct tctggtggac cctgagcaac
      961 tgcatttgct ggaatcgctg atgaaccaag tggccttggc gatcgaacga gcttgtttat
     1021 ccgacgaagc tcaacaggcg catgtacggg cggaaaccga gcgcatgcgt aacgccatac
     1081 tgagttcggt ctctcacgac ctgcggacgc cgctggccac gatcacgggc gccgccagta
     1141 gtttggcggc ggaacgggga gaattcgacc ctgttgctcg gcgcgagctt gcccaatcca
     1201 tctatcggga agcggagcgc cttgaccgtt tgctgaagaa ccttctcgat atgatgcgca
     1261 ttgaagcagg ggttgtgcag ctcaagaagg aatggcattc ggtggatgaa gtggcgggag
     1321 cggttttggc cggacttgat gggcgattac acgatcatgc cgtcaatacg tcgtttcctg
     1381 cagatcttcc gctggtgctt gtcgacggag tgctcctgga acaggtcgtc atcaatctta
     1441 tcgagaacgc cgtgaagtat gcgcctgcga gaacgatgat cgagctggct gcgtcggcga
     1501 cggatcgaga aatcgttgtg gaagttgcag atcgaggacc gggcatcccc gttggggagg
     1561 aatcccatat cttcgacaaa ttctatcgtg caaagcctgc gagagaggga ggagtcgggc
     1621 tggggctgac gatttgtcgt ggtattgtag aggcgcatgg cggtcgtatc tgggctgaga
     1681 atcgctccgg aggcggagct ctctttcgct tctcaatccc gttgcctaat cagcagccgc
     1741 ctatagaaac cgaacagccg gaggctcggg acgcgtaatt ctggtcaagc gagccggcag
     1801 gtcctatgtc acaggacgcc acgatacttc tcatcgaaga tgaacctgag atccgtcggt
     1861 tcctgcggac gatacttccc gcgcatggct ttcgcctcta tgaagccgcg acgggacagg
     1921 atgggcttac agagacgaaa gcgaggaatc ccgatctcat tctcctcgac cttggtctgc
     1981 ctgatctcga cggaggcgag gtcatccgcc aggtgcgaga gtggaccgcg acgcccatca
     2041 tcgtgctgtc tgctcgtgat caggaacagg cgaaggtggc ggcactcgat ctcggcgccg
     2101 acgactatat cactaagccg ttcggagtga acgaactgct cgcacgggtg cgcaccgcat
     2161 ttcggcatgc agcccgaacc ggtgatggcg gggaatcggt gtttgtgctc ggcgatctca
     2221 aggtggatct cgggcgacgg caggtgtttg tctcagggaa ggaagttcac ctcacaccga
     2281 tcgaatacaa gctgctcacc acgttgatcc gctatgccgg caaagtgttg acccaccgcc
     2341 agctgttgaa agaagtctgg gggccgcttc atgtggatga agggcactac ctacgggtct
     2401 atatgcgcca attgagaaat aaactggaac gcaatccggt gcatccccgt tatcttatga
     2461 cagagctggg agtcggatat cggcttcgga cagaatgatc tcactcttct tcatgctttc
     2521 ttgatactcc cctggccggt ttttatcccg ttttgatacc tgacgaagta tagtttgtga
     2581 gccgggcttg ggagtctgtc tcccacattt cgtacggggc attatgaggc aagcaggcgc
     2641 tgcctattca tttatggaca atgcgaaggc ggtcaatcat gaatacggat ggcgcaagca
     2701 tatcagcctg ctgttaatcg cgtgggggct attcacgacg gtccctattt gtgtggctca
     2761 aactcaagac tcgaatcagg cattgaaaga aaccatggca cctatacccg gtgcaaaccg
     2821 tcccacagac gctgctacga ccgactggca ctatggggct tacgtagacg tcggttacat
     2881 cggcaatttc aattttcccg acaaccatct ctggcgtagt cgcaccactg caaaccacga
     2941 caatgagctt tcgcccaaca tggggctggc gtatgtgcga aaggatgcga gtgtctcctc
     3001 acgctggggt atggagcttg gtttccaagg cggccgggat acggaagagt tcgcattttt
     3061 ggtgggtgaa aaacgagtcg atggagcgga tgtgctgagg cacgttcatc gtgcgaacgt
     3121 gtcatatctg gctccggtcg gaaaaggatt gacattcacg gcaggtctat tcaacagctt
     3181 gatgggatat gaatcactct atgcaagaga caacgccaac tatacgagat cgtggatcgc
     3241 ggacaatacg ccttacatga tgtttggggt gaatgcacag tatcctgtga acgacaatct
     3301 cacagtcacc gcctttgtcg tgaacagtta ttaccacctg gcgcatccga acgatctccc
     3361 gagctatggc ggaagatggg catggaaagt aacgccgagg ttaacattca tacaaacact
     3421 ctacggaggg ccggatcaga cggatacgtc cctggagttc tggcgtgtat atgggaatca
     3481 cattctgga
//
LOCUS       DATNDD010000368         2930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1114441, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000368 DATNDD010000000
VERSION     DATNDD010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114441"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VN647_08905"
     CDS             complement(<1..394)
                     /locus_tag="VN647_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWG97187.1"
                     /translation="MPRNTLGADFLVIGSGVAGLRAALNLCRVGRVIVLTKGHPLQSN
                     SIFAQGGVAVALSEEDDVAIHLTDTVKAGHGLCRREAVRVLVEEGPDRIQELIRWGAK
                     FDKTGGKFAFAREAAHSRSRILRARGDAT"
     gene            complement(397..858)
                     /locus_tag="VN647_08910"
     CDS             complement(397..858)
                     /locus_tag="VN647_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97188.1"
                     /translation="MLTVVAVGCPSKTIRYPAEHERLLRIDQALESLRSAYERKDRSG
                     FQSMLLPQDQLDELRRQVDLDLEAFSTISLEFKIERVVIEKDDIDVFIHWQGTWKRDA
                     NDMGIRQRGHARLQWVGTHSILLRSAQGDLPFGMKTKQMLSESPSPSSQPR"
     gene            1132..1350
                     /locus_tag="VN647_08915"
     CDS             1132..1350
                     /locus_tag="VN647_08915"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97189.1"
                     /translation="MAEGRKVRIRVRTVNCTYVGDFLVPPMRNRVSDAINEEARLFIS
                     LTDVVIDDKDRSDFVAINKNLIESIAQL"
     gene            1423..>2930
                     /locus_tag="VN647_08920"
     CDS             1423..>2930
                     /locus_tag="VN647_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG97190.1"
                     /translation="MVIFKRFLPFVRPHLARLILAGLLVSGVAIINLALIRLAGTLWD
                     IITIRHDADQMTRSIGLFLGLVILQGLCSMGHSYLTAWISQEVIADFRKHLFGHLQTL
                     TVSFFARRRTGELLSRLMTDVTVIQSIATETPIDGVKQLVTFVGGITFLLMMNWQLCL
                     LILILLPLLVLVAKVFGRRLKSLSTSIQDHTAALSTLVEEVISGIRIVKSFVQTQREQ
                     ARFADQVDQTLHLTLRRAGIMAVFIPVISLLTFSSATAVLWYGGKQVIDGVVTPGDLF
                     AFVLFAGILIGPFSSAARVFAQIKEAQGATQRVFEILDARPDICDRSDAQTLVTVDGH
                     VRMEGVNFSYDTRHPVLSNLSFEARPGELVAFVGPTGAGKTTVINLLHRFYDPTEGRI
                     TIDDIDLRHVTLESWYRQIALVPQETILFGGTILDNIRYGNMAANEASVLEASKAAHA
                     HDFITSLPDEYQTLVGEKGVNLSGGQRQRIAIARAILKNPRILLLDEATSSL"
BASE COUNT          637 a          840 c          791 g          662 t
ORIGIN      
        1 ctgtcgcatc gcctctcgca cggaggattc ggctgcggct gtgagccgct tcacgggcaa
       61 aggcgaattt cccaccggtt ttgtcgaact tcgcgcccca gcggatgagc tcttgaattc
      121 gatccggccc ttcctcgacc aaaactcgaa ccgcttcacg acgacacagg ccgtgtccgg
      181 ctttgacggt gtccgtcaaa tggatggcga catcatcctc ttcgctcaag gccacggcaa
      241 cccccccttg cgcgaagatg gaattgcttt gtaagggatg gcccttggtg agaacgatca
      301 cgcgacccac tcggcagaga ttcaaggctg cgcgtagccc ggcgactccg ctgccgatga
      361 caagaaaatc tgctcccagc gtattgcgcg gcatcgttat cgtggttggg acgacggtga
      421 gggagattcc gacaacatct gtttcgtttt cattccaaag ggcaaatctc cctgcgcgct
      481 acgcaagaga atcgagtgcg taccgaccca ttgcaaccgt gcgtgacccc gttgccggat
      541 acccatgtca ttggcatctc tcttccacgt cccttgccaa tggatgaaga cgtcgatatc
      601 gtctttttcg atcacgaccc gttcgatctt gaactcgagg gaaatggtgg aaaaggcttc
      661 gaggtccaga tcgacctgcc gtcgcaattc gtccaactga tcctgcggca acagcatgga
      721 ctggaacccg gaccgatcct ttcgttcata ggcgcttcgt aaggattcca acgcttggtc
      781 gatacggagg agtcgctcgt gctctgctgg atatcgaatg gtcttcgagg gacacccaac
      841 tgctaccacg gtgagcatga ggagccacac gatggatgtg ggctgtgaca aaccaatccg
      901 cttaagaggc atggccgtgc agtatagcat aaggggaaaa ggcgccgtga gcttgcattt
      961 tcagcggaaa gcttttagac tcggccctcc ttaggagata ctaatatgtc aagcgtcttg
     1021 aaaaaacgac gaaagaagat gcgcaagcat aagtacaaga agctgcgcca acgtcagaaa
     1081 tttcttcgcc gaaaaagcta aactcagccg gcgtggcgtg aggagggaac cgtggccgag
     1141 gggaggaaag tccgtatccg cgtacggacg gtcaattgta cctatgtggg agacttcctg
     1201 gtccctccga tgcggaatcg tgtctccgat gcgatcaatg aagaagcacg tctcttcatc
     1261 agcttgaccg atgtcgtgat cgacgataag gaccggtcgg atttcgtcgc gatcaataag
     1321 aacctgatcg agtcgatcgc tcaactctag tcgtttcgtc cccaccccag tcgacgtaag
     1381 ccggaaacac gattccgtgt ccaatcacac cgcccgagca ccatggtgat cttcaaacgt
     1441 ttcctcccgt tcgtgcgccc tcatttggcg cgtttaatac tggccgggct cttggtgagc
     1501 ggggttgcga tcattaatct cgccttgatc cggctagccg gcactctctg ggatatcatc
     1561 acaattcggc acgatgccga tcagatgacc cggtcgatcg gcttattcct gggcctggta
     1621 atcttacagg ggctatgctc gatgggtcat agttatctga ccgcgtggat ctctcaggaa
     1681 gtcatcgccg actttcgcaa acatctcttc ggtcatctgc agacgctgac agtcagcttt
     1741 tttgctcggc gccgtacggg agaattgctc tcccgattga tgaccgatgt gacagtcatc
     1801 caatccatcg cgactgaaac gccgatcgac ggagttaaac aactcgtgac gttcgtcggc
     1861 ggcatcacgt tcctgctcat gatgaattgg cagctctgcc tcctgatcct gatccttctt
     1921 ccgttattgg ttctggtcgc caaggtattc ggccgcagat tgaaatctct ctccacttcg
     1981 attcaagacc acaccgccgc actcagcacc ctcgtcgaag aagtgatttc cggcattcgc
     2041 atcgtcaaat cgttcgtcca gacgcaacgg gaacaggctc gttttgcgga tcaagtcgat
     2101 caaacgctcc atctcaccct ccgtcgagcc ggaatcatgg ccgtcttcat cccggtaatc
     2161 agcctcctga cattctcatc ggcaacggcg gtgctgtggt acggaggcaa acaagtcatc
     2221 gacggagtcg tcacgccggg tgacctgttc gcctttgtct tgttcgccgg catcttgatc
     2281 ggccccttca gttcggcagc gcgcgtcttt gcgcagatca aagaggccca gggagcgacg
     2341 cagcgtgtct ttgaaatact ggatgctcgg ccggacattt gcgatcgatc cgatgcccag
     2401 acactggtga ccgtcgatgg ccatgtgcgg atggaaggcg tcaacttcag ctacgatact
     2461 cgtcatccgg tgttgtcgaa cctgtcgttc gaggccaggc cgggcgaact ggtcgccttc
     2521 gtaggaccga cgggcgccgg caagaccacc gtgatcaacc tgctgcaccg cttctacgat
     2581 ccgacggaag gtcgcatcac gatcgacgac atagacttgc gccatgtgac ccttgagagt
     2641 tggtaccgac agattgctct cgttccacaa gaaacgattc tttttggggg taccatcctc
     2701 gacaacatcc gctacggcaa tatggcagcg aatgaagcct cggtattaga agcgagcaaa
     2761 gccgcccatg ctcatgactt catcacgagc ttgccggacg agtatcaaac cttggtgggc
     2821 gagaaggggg tcaatctgtc cggcggacaa cgccaacgaa tcgcgattgc ccgggcgatc
     2881 ttgaagaatc ctcgcatttt gttactggac gaagcgacct cgtcgctgga
//
LOCUS       DATNDD010000369         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2775818, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000369 DATNDD010000000
VERSION     DATNDD010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775818"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VN647_08925"
     CDS             complement(<1..722)
                     /locus_tag="VN647_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWG97191.1"
                     /translation="MLPSLTAVFVLGLLCGAQISFFPLSVIALLAGIAVGFSILERVG
                     YIDSPSALLLYSSLLSGLGYWTLTTPTLEFSRMSPPLHETVQVSVVGRVIAPVQHSVG
                     RQTIMLQTDDSASRRIRLVWRDPGITLHDGDRISFRGKLHRPRGSLNPGGFDYASYMD
                     RQGIDFLATVTGAQAVALLDAEPQIGLWSFWNRIDHWRAMIRAAAISTLSQPTRGLFL
                     GMITGERGYLEQELQDWFMVTGT"
     gene            complement(758..2110)
                     /gene="glmM"
                     /locus_tag="VN647_08930"
     CDS             complement(758..2110)
                     /gene="glmM"
                     /locus_tag="VN647_08930"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWG97192.1"
                     /translation="MMRKLFGTDGVRGVANLDPMTSEMAMQLGRAAAHIFMRRAGRHQ
                     IVIGKDTRISGYMLESALMAGICSMGVDVLLVGPMPTPAIAFLTRSLRADAGVVISAS
                     HNPYQDNGIKFFSSDGFKLPDEVEARIEELIVSDEISHLRPTADLIGKAYRIDDAEGR
                     YIEFAKRSLPKDLDFQGIKLVVDCANGAAYKVAPTVLRELGATVEVIGNKPDGMNINA
                     DCGAVHPELLQESVRRYKADLGIALDGDADRAVFVCEQGTVIDGDHVMAALGLDLHRN
                     GLLAKQTLVGTVMSNFGLELSMLKAGVKLIRTPVGDRYLLERMLGEGYNFGGEQSGHF
                     IFLDHNTTGDGLISALQMLSLVKRTKKPLSELAEAMTAVPQVLVNVHVTKKPRLESIP
                     DIDCAMQESERRLNGCGRVLIRYSGTEPLLRIMVEGEQATMVEEVAEDLARVVRKHIG
                     "
BASE COUNT          537 a          673 c          574 g          486 t
ORIGIN      
        1 gttcctgtca ccatgaacca gtcttggagc tcttgttcga gatagcctcg ttcaccggtg
       61 atcatgccca gaaatagccc tcgggtgggt tggctcaatg tgctgatagc cgctgcacgt
      121 atcatggccc tccaatgatc gatccggttc cagaaactcc acaacccgat ttgaggctct
      181 gcatctaaca gcgccaccgc ctgagcaccg gtcaccgtgg ccagaaagtc gatgccctga
      241 cgatccatat aggaagcata gtcgaatcca cccgggttca acgaccctcg aggacggtga
      301 agttttcctc gaaacgaaat acgatcaccg tcatgaagcg tgatgccagg gtcgcgccac
      361 acaaggcgta tgcgtctcga tgccgaatcg tctgtttgaa gcatgatcgt ctgacgcccc
      421 acactgtgct gaactggagc tataacccga ccgacgaccg aaacttgaac agtctcatga
      481 agcggcggcg acatccggga aaattccaac gtcggtgtgg tgagagtcca gtaacctagt
      541 ccggagagaa ggctggaata cagaagcagt gcagagggtg agtcgatata accgacccgc
      601 tcgagaatgc tgaatccaac ggcgatgccg gccagcagag ctatgacaga gagcgggaaa
      661 aaggagatct gggccccaca cagaagaccg aggacaaaca ctgctgtgag ggatgggagc
      721 atgagcttcc ctcatggacc gggggaagag ggaacgctca gccgatatgt tttcgtacaa
      781 ccctggcaag gtcctcagcc acttcctcga ccatggtggc ctgttctcct tccaccatga
      841 tccgcaacag cggctcggtc ccggaatatc tgatgaggac tcgcccacac ccgttcagac
      901 gacgttcgct ctcctgcatg gcgcaatcaa tgtcgggaat cgattccaat cgcggcttct
      961 tcgtgacatg cacattcacc aacacttgcg ggacggcggt catcgcctcg gccaactcgg
     1021 ataacggctt cttggttcgc ttcaccaatg ataacatctg cagagccgag atgaggccgt
     1081 ctccggttgt attgtggtca aggaatatga agtgacccga ttgttctcct ccgaagttat
     1141 agccttcacc caacattcgt tcgagcagat accgatcacc gaccggcgtc cgaatcagct
     1201 taacgcctgc tttcaacatc gacagctcca gtccaaaatt gctcatcacg gttccgacca
     1261 aggtctgctt ggctaggagt ccgtttcgat gaagatccag ccccaaggcc gccatcacat
     1321 gatcgccatc gatgaccgtt ccttgctcac agacaaacac ggcccgatcg gcatcgccgt
     1381 ccaacgcaat accaagatcc gctttgtaac ggcgtaccga ctcttgcaga agttcaggat
     1441 ggacggcgcc gcaatcggcg ttgatattca tcccgtccgg tttattgccg atgacttcaa
     1501 ccgtggcgcc caattccctg agaaccgtcg gagcgacttt gtatgccgca ccatttgcac
     1561 aatcaacgac gagcttgatt ccctgaaaat ccaaatcctt cggcaatgac cgctttgcaa
     1621 attcaatata acgcccctct gcatcgtcga tacggtaggc tttgccgatg aggtctgccg
     1681 tcggtcgaag atgactgatt tcatccgata caatcagttc ttcaatacga gcttccactt
     1741 cgtcggggag cttgaagccg tcgcttgaaa agaatttgat tccgttgtct tggtatggat
     1801 tgtgcgacgc cgaaatgact acccctgcat ccgcccgtaa acttctggtc aaaaacgcga
     1861 tcgccggagt gggcatcggg ccgactagca gtacatcaac ccccatcgag cagattcccg
     1921 ccatcaaggc agactccaac atatatccgg aaatacgggt gtctttgccg atgacgatct
     1981 gatggcgccc cgctcgtcgc atgaaaatat gtgcggcggc tcgtccgagc tgcatcgcca
     2041 tttcactggt catgggatcg agattggcaa cccctcgaac gccgtcggtt ccaaataatt
     2101 tacgcatcat gctcctgtcc gcaagatgac cagcgtggat tcaacgctgc agccatcttg
     2161 actacgtcga tcatcatggc aacatcgtgg acacgcacaa tgtgagcgcc gcgatcgacc
     2221 gccaatgcca ctgccgctgc ggtcccccat tctcgatgct caacaggccg
//
LOCUS       DATNDD010000370         3198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_736097, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000370 DATNDD010000000
VERSION     DATNDD010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736097"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..694
                     /locus_tag="VN647_08935"
     CDS             197..694
                     /locus_tag="VN647_08935"
                     /inference="COORDINATES: protein motif:HMM:NF039808.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6527 family protein"
                     /protein_id="HWG97193.1"
                     /translation="MGRTDSVGDPCGIATRDYGAGVLVGGAEVKLTDLEPQFVRYETK
                     DEVRDFIVGDPATWHERGNPVEYRVRLCESKVYVNTLAEAQGIMFLCPVCFAANGGNV
                     GTHSCEVTFEGRGALPSQGTQNKQGPVRWNVSGDSYENLTTTPSIQLIGGCNWHGYIT
                     NGDVT"
     gene            774..1037
                     /locus_tag="VN647_08940"
     CDS             774..1037
                     /locus_tag="VN647_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97194.1"
                     /translation="MHAMRETGLEIVPEVWEHEGLHAPRLSVLAVLPVMSQPSDPRPT
                     PIIVMAIAAALMAAWLVIWSAWHEPTKGTGPRVIRSDARVLVW"
     gene            1031..1525
                     /locus_tag="VN647_08945"
     CDS             1031..1525
                     /locus_tag="VN647_08945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWG97195.1"
                     /translation="MVTRRGLITLVAGLLCSTLAAAPEPIRETRLEIVVRPTEIDGND
                     HANNARYLEWLQWGRDDWYTQKGLTRDLFKRWGVTPVTVNINIDFKRECLQGEALTIA
                     SKIDRMGDKSYAFFQSVRKANGETAAEAVTTLVVMDTATRKSRAIPDEMLKVLAPAAE
                     KVQP"
     gene            1522..1734
                     /locus_tag="VN647_08950"
     CDS             1522..1734
                     /locus_tag="VN647_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97196.1"
                     /translation="MKLIDQSAFAIVSPQGRLRAAQPNEYEAWAQFLNWPKIHPQGTT
                     LDEAIMAWKAIGYTCRKVAVVEMSEE"
     gene            1731..1922
                     /locus_tag="VN647_08955"
     CDS             1731..1922
                     /locus_tag="VN647_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97197.1"
                     /translation="MTNALNQPMTEKNVTDRLLFINSLLVDLLVLPKVHRASEELTTL
                     IGEIAQNGMAPEDPSKHAF"
     gene            1912..2049
                     /locus_tag="VN647_08960"
     CDS             1912..2049
                     /locus_tag="VN647_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97198.1"
                     /translation="MLFDDTDRVVKKPVFWFVIGVTVSVITTAVAGFLAVTSSWERVR
                     P"
     gene            2046..2261
                     /locus_tag="VN647_08965"
     CDS             2046..2261
                     /locus_tag="VN647_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97199.1"
                     /translation="MTVDEAVKVLNNHKHDDNSKWYLDGKPGAPVHYVRGEGQYEAFS
                     EFEAIAIAEKYQRLDAERDDEEGPVQG"
     gene            2258..2461
                     /locus_tag="VN647_08970"
     CDS             2258..2461
                     /locus_tag="VN647_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97200.1"
                     /translation="MTIDEAAKVLNEHTYRGRSNWVHMEIFAISKDKDGEEILTHFEA
                     VALAQAYQRLDAEGDDYERRGQT"
     gene            2458..2757
                     /locus_tag="VN647_08975"
     CDS             2458..2757
                     /locus_tag="VN647_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97201.1"
                     /translation="MISTNPADFPALLAKAQAWVAGAADKFYARKLAELGGLKAMALK
                     HIYPEIRAELPAMTEDGIGTFLDGFVDMTGADMMKMLEAAIEARKIAKMQAKGLR"
     gene            2973..>3198
                     /locus_tag="VN647_08980"
     CDS             2973..>3198
                     /locus_tag="VN647_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97202.1"
                     /translation="MADEPKKKTGRPLKEIDGDQVYKLAKMGCTQEEIADYFGCASHS
                     TISERFRQDYELGRAASKTSLRRMQWKSAIS"
BASE COUNT          732 a          834 c         1025 g          607 t
ORIGIN      
        1 ctcgaacagg ccaagaaagc gcaccagcgg atcgagattc aggttcgtga gaatgcgatc
       61 gtgatccgcg ttctggcgat caaggccggt atcacccgcg ccgagatccg caatgcgatt
      121 agaccaccgg cggacgattg agcatgtaca ccgacgaccc cggccccctg ttcgatcgca
      181 tccccgatca cccctcatgg ggcgaacgga ttcggtcggt gatccttgcg gcattgctac
      241 tcgtgattac ggggctggtg tgctggttgg tggtgccgaa gtgaaactga ccgatcttga
      301 gcctcaattt gtgcggtacg agaccaaaga tgaggtcaga gacttcatcg tcggtgatcc
      361 cgcgacgtgg catgagcgag ggaatcctgt cgagtatcga gtccggctat gcgagtccaa
      421 ggtctacgtg aacacgcttg ctgaagcgca ggggattatg tttctgtgcc cggtgtgctt
      481 tgcggccaac ggcggcaacg tgggaacgca ttcctgcgaa gtcacgttcg aaggccgcgg
      541 cgcgctaccc agtcaaggca cgcagaacaa gcaagggcca gtgcggtgga atgtctcggg
      601 tgactcatac gagaatctga ccaccacgcc atcaatccaa cttatcggtg gatgcaactg
      661 gcacggctac atcacgaacg gagatgtaac ctgatgcctg ctttcaacga cgcgcgatgc
      721 gagaagtgtg atgcgcgaat cggctggttc ggcgagatga gcgataagcc gccgtgcacg
      781 cgatgcgaga aacgggactc gaaatcgtgc ccgaagtgtg ggaacacgaa ggtctacacg
      841 cgccacggtt atcagtgctt gcggtgctcc cggtgatgtc ccaaccctcc gacccccgcc
      901 ccaccccgat catcgtcatg gccattgccg ccgcgctcat ggcagcatgg ctcgtgatct
      961 ggagtgcgtg gcatgagccg acgaaaggaa cggggccgcg ggtgatccgg tcggatgcga
     1021 gggtgttggt atggtgacac gccgcggact catcacgctc gttgctgggc tgctctgctc
     1081 gacgttagca gcggctcccg aaccaatccg agagacgcgc cttgagatcg tcgtcaggcc
     1141 cacggagatc gatggcaacg accacgcgaa caacgcccga tacctcgaat ggctccagtg
     1201 gggacgggac gactggtata cgcaaaaggg actgactcgc gacctgttca aaaggtgggg
     1261 cgttacgcca gtcacggtaa acatcaacat cgattttaag cgcgaatgcc tacaaggtga
     1321 ggcgctgacg atcgccagca agatcgaccg catgggcgac aagagctatg ctttcttcca
     1381 atcggtccga aaggctaacg gcgagacagc ggccgaggcc gttacgacgc tggtagtcat
     1441 ggacacggca acccgcaaga gccgggccat tcctgatgag atgctgaagg tgctggcgcc
     1501 ggctgcggag aaggttcagc cgtgaaactt atcgaccaat ccgcctttgc catcgtctcg
     1561 ccgcaaggca ggctccgtgc cgcgcagccg aacgaatacg aggcgtgggc gcagttcttg
     1621 aattggccga agatccatcc acagggcacg acgcttgacg aggcgatcat ggcttggaag
     1681 gccatcggct acacctgccg caaggtagct gtggtggaga tgagcgagga atgactaacg
     1741 cgctcaatca gcccatgacc gagaagaacg tcaccgatcg gctgttgttc atcaacagtc
     1801 tgttggttga tcttctcgtg ctccccaagg tgcaccgggc atccgaggaa ctgacgacgc
     1861 tgatcggcga gattgcccaa aatggaatgg cccctgaaga tccgtcgaaa catgcttttt
     1921 gacgacaccg accgcgtggt gaagaagccg gttttctggt tcgtgattgg ggttaccgtt
     1981 tcagtgatca cgacggctgt ggcgggattc ctggcggtga catcgtcgtg ggagagggtg
     2041 agaccgtgac tgttgatgag gcggtgaagg tgctgaataa ccacaagcac gacgacaact
     2101 cgaaatggta tctcgacggg aaacccggcg ctccggttca ttatgttcgc ggcgaaggtc
     2161 agtacgaggc atttagcgaa tttgaggcca tcgccatcgc ggagaagtac cagcgcctgg
     2221 acgccgagcg ggatgatgag gaagggccgg tgcaggggtg accatagacg aagcggcgaa
     2281 ggtgttgaat gagcacacgt atcgaggccg atcgaattgg gtgcacatgg aaatattcgc
     2341 catttccaag gataaggatg gcgaagagat cctgacccat ttcgaagccg ttgcattggc
     2401 tcaggcatac cagcgcttgg atgctgaggg ggatgattat gaaaggcggg ggcagacatg
     2461 atctccacca accccgccga cttcccggcc ttgctcgcca aggcacaagc ctgggttgcg
     2521 ggtgcggccg acaagttcta cgcccgcaaa cttgcggaac ttggcggcct gaaagcgatg
     2581 gccctgaagc acatttatcc cgagatcagg gccgaacttc ctgccatgac cgaggacgga
     2641 atcggaacgt tcttggatgg cttcgtagac atgacgggcg ctgatatgat gaagatgttg
     2701 gaagcggcga tcgaagcaag aaagatagcc aagatgcaag cgaaggggct ccgctgatgg
     2761 gctggatcgt cgcggggatc attgcaatgg ttgccgtgtt cctgtggttg gaatggaccg
     2821 ggatgcttga agggatttga atactttggg cgttcctcgc gggtattgtc gtgggagcgg
     2881 tggggctagg atcttggctg tggtatcggc tgtttgttaa ggatgcgaac ccgaggccag
     2941 tgaggaagcc gaaggccgag agagcgtgaa atgtggcaga cgaaccgaaa aagaagaccg
     3001 ggcgaccact caaggaaatc gatggtgatc aggtctacaa acttgcgaag atgggatgca
     3061 ctcaggaaga gatcgcggac tacttcggat gcgcttcgca cagcaccata agcgagcgtt
     3121 ttcgacaaga ttacgaacta ggacgcgcag cgtcgaaaac cagccttcgc cgaatgcaat
     3181 ggaagtcggc tatcagcg
//
LOCUS       DATNDD010000371         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1022252, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000371 DATNDD010000000
VERSION     DATNDD010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022252"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..571)
                     /locus_tag="VN647_08985"
     CDS             complement(107..571)
                     /locus_tag="VN647_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97203.1"
                     /translation="MRRSPAEVVKRYVLLDQKGARLDAPSFETLTPYIEWKEEPAWGR
                     VVVVEKPTVPEDYRKWEILNNLEVIIPVTFHVLGAVYLKTAIFVPEETTEEVRFHVKV
                     VDGYWRIVSPVIPPHVGLKRMVSFAREAEAHEQDDAQRIVLAALIDSLRKVE"
     gene            984..1412
                     /locus_tag="VN647_08990"
     CDS             984..1412
                     /locus_tag="VN647_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HWG97204.1"
                     /translation="MFTLRWRWIRVIGLLILPCTFSLTFPVSIAAADLGNRPPNVDTI
                     ISSPGQIEPPSSLATPSPKVYDLLKQLEERSGRPLPGYIGGRDFQNRERRLPRGHYRE
                     YDVNPKIRGRGRDAERIVIEQRTGKAYYTGDHYRTFVPLN"
     gene            1424..1972
                     /locus_tag="VN647_08995"
     CDS             1424..1972
                     /locus_tag="VN647_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HWG97205.1"
                     /translation="MAGNSTLQTHLCNATPPWSALLVVPRGTSPSGLVKTPPGYAMRT
                     IQGKKCRTPSGLFGEFARALAFPDYFGHNWDALEECLADLEWLPANGYILLVTDTQAV
                     LPEDEEEYDTLLEILDDAGEAWSKGQTAEGRSAPFHVVFVIAEEDKSKRKRWELEEFS
                     SIESETPKAKTSPKRSAKRHRK"
     gene            2538..2966
                     /locus_tag="VN647_09000"
     CDS             2538..2966
                     /locus_tag="VN647_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWG97206.1"
                     /translation="MRTSRSAKLFTDAQQLIPGGVNSPVRAFRSVGGQPRFIARAKGS
                     RLYDVDRNVYIDYVLSWGPMILGHAPSAVITAIKSAAERGTSYGAPTESEVSLAREIR
                     NAFPSMGKVRLVSSGTEAVMSAIRVARGFTKRTGVMKFEG"
BASE COUNT          709 a          878 c          763 g          635 t
ORIGIN      
        1 cccagctcga ctagggtgaa gttgacggat tgcgcgatgt cccactttga ttcgatcagc
       61 gtaccgtcca agtcgaaaat catcaaatcc accggagttt tagccattac tcaacctttc
      121 tcagagagtc aatgagggcg gcaagcacga tgcgttgagc gtcatcctgt tcatgggctt
      181 ccgcctctcg cgcgaagctc accatccgtt tcagtccgac gtgcggagga atcaccggcg
      241 aaacgatacg ccagtagcca tccacaacct tcacatgaaa acgaacttct tccgtcgttt
      301 cctcaggcac gaagatcgca gttttcagat agacggcccc gagcacgtga aatgtgacag
      361 ggatgatcac ttccagattg ttgaggatct cccactttcg atagtcctcc gggacggttg
      421 gtttctcaac gacaacgaca cggccccatg caggttcttc tttccattca atgtagggag
      481 tcaaggtttc gaatgatgga gcgtctaaac gggctccctt ctggtccaaa agcacataac
      541 gcttcacgac ttccgccgga gaacgtctca tcgaaccgct cggattcagc tgtgcgttcg
      601 aagggacggc cagaacgagg acagccagag tgactagata atggacgagg cgcctcagta
      661 aaggattcac gcgtctaatc ggatggatct cacacagcgc acgcatagat ctcgcacgat
      721 gaccctggac gtacgttcga cacttgttta ttcagatgta catttcagcc gccccattct
      781 agcaaacgga actgaagaca tccaggtaat gaggtggaac cgctccgctt gacagtaatg
      841 ctctggtggc tcttcagcga cacctcgccg aagaatgtcc tccctacgta gacgggtgat
      901 ggcgcctgcg tcgtttgacc ttcttgaaaa gagaatgcta cggtcgtctt tcagggcaac
      961 gtcttgccaa ggaagaagag cctgtgttca cccttcgatg gagatggatt cgggtcatcg
     1021 gtcttctcat cctgccgtgc acgttcagcc tgacgttccc tgtctccatc gccgctgccg
     1081 acctcggtaa cagacctccg aatgtcgaca ccattatcag ctcgccgggt caaatcgagc
     1141 caccgtcctc gctcgcaaca ccttccccaa aagtctacga cctgctcaaa cagctcgaag
     1201 agcgaagcgg gagaccgctg ccgggctaca tcggtgggcg tgacttccaa aaccgagaac
     1261 gacgtcttcc acgaggccac taccgggagt atgatgttaa tccaaagatt cgaggccgcg
     1321 gacgcgatgc cgaacgaatc gtcatcgagc agcgaaccgg aaaagcctac tataccggag
     1381 accactaccg gacctttgtc cctcttaact gacgaacatc gctatggcag gaaactctac
     1441 cttacagacc catctttgca atgccacacc tccctggtcg gctctccttg tcgttcctcg
     1501 cgggacctca ccctcggggt tggtgaaaac gccgccggga tatgccatgc gtaccatcca
     1561 agggaagaaa tgccgaactc catcaggtct cttcggtgag ttcgcgcggg cactcgcctt
     1621 tccggattac ttcggacaca actgggacgc actcgaagaa tgtctggccg atttggagtg
     1681 gcttccggcc aacggctata ttttactcgt taccgataca caagccgtgc ttccagagga
     1741 cgaagaagag tacgacacat tgctggaaat cctcgacgac gccggcgaag cctggagcaa
     1801 gggtcaaacg gccgagggtc ggagcgcccc attccatgtc gtttttgtaa tcgccgagga
     1861 agacaaatcg aagcggaagc gctgggagct ggaagaattc tcctctatcg agtcggagac
     1921 acccaaggcg aagactagcc cgaaacgttc agccaagcgg catcgaaaat agaacctacg
     1981 acaacgagct gaacaggcat accgattaga aggaactagc tgcaaatttt tgggagacca
     2041 tctctcaagc cagcatagaa ccgcacgacc gatcgcacca tccggcgaat agacgaagac
     2101 tgatcattca gacttttgcc ggacgttcgg catgaactta cactcccttg atacccggtc
     2161 catcgagctc gcaccacctt ctcgtgctcg atgcagatac tatgtgtctc tcggcggtcc
     2221 tcgaggggag ctttttctcc cacgagtccg acccgacctt ctccacgaca ccatgaacag
     2281 atgacttgca tatccgtctc actattctgc aatcgacttc tgagcaagaa tcgcgccgtc
     2341 cggcaaaaca gcgaccaggg taaaaaccaa gagaacttca ccttcctgtc gaaccttaac
     2401 cgccgccacc gtatctgaat cgataggact gtatcgttaa gacattgttt gacctcccat
     2461 ggcgctcttc gatcggatcg cacaacaatg agtttcttga ccggctctta cccagcgtgt
     2521 tagggtgcgc ccccattatg agaacatccc gctctgccaa gctcttcacc gacgctcagc
     2581 aactcattcc tggtggcgtc aatagtcctg ttcgggcctt ccgttcggtc ggcgggcaac
     2641 cgcgcttcat cgcacgggcg aaaggatcgc ggctctatga tgtggaccgc aacgtctata
     2701 tcgactacgt gctgtcatgg gggccgatga ttttaggcca tgctccttcc gcagtaatca
     2761 cggcgatcaa gagcgcggct gagcgaggca cgagttacgg agcgccgact gagtcggaag
     2821 tatcactcgc tcgagaaata cggaacgcct tcccttccat gggaaaagtt cggcttgtca
     2881 gctccggcac cgaagccgtc atgagcgcga tccgtgtggc gcggggattc accaagcgga
     2941 ccggcgtcat gaaattcgaa ggctgatatc acggtcacgg cgact
//
LOCUS       DATNDD010000372         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1869145, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000372 DATNDD010000000
VERSION     DATNDD010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869145"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VN647_09005"
     CDS             complement(<1..185)
                     /locus_tag="VN647_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG97207.1"
                     /translation="MNAHDPLVSIVTPVYNGEKYLAECIESILAQTFPRWDYTIVNNC
                     STDRSLEIAECYAQKDS"
     gene            complement(206..1687)
                     /locus_tag="VN647_09010"
     CDS             complement(206..1687)
                     /locus_tag="VN647_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HWG97208.1"
                     /translation="MNDLKERTLRGGAAKVFAQGANFFLRLGSLMVLARLLDPKDFGL
                     VGMVTAVTGVFSLFRDAGLSMATVQRTTISHKEVSTLFWLNMAVGVILGCLSLAIAPI
                     LVSFYQEPRLFYVTAVLGAGFLFNAAGVQHSALLQRQMRFVTLSVIETVALLISIAVG
                     IGMALNGFGYWALVGMAVALPAVSTICLWLLGTWVPGMPHRRAEIGSMVRFGGTTTLN
                     SLVVYVAYNLEKVLLGRFWGAETLGYYGRAYQLVSIPTENLNSATGGVVFAALSRLQN
                     EPERLKNYFLKSYALVLSLTLPISVACALFADDIIFLVLGPKWQDVVPIFRLLTPTIL
                     IFALINPTYWLLVSIGLVGRSLKLALVIAPLVITAYILGLPYGPQGVAFAYSAVLTLW
                     VVPHIAWCIHGTMISWKDLLQTVGRPFLSTMVAAAFASAAQFFYDQSLSPFPRLILGA
                     TILMVSYSWMLLVVMGQKSFYLDLLRGLRKRSSVGGKESLELA"
     gene            complement(1861..2940)
                     /locus_tag="VN647_09015"
     CDS             complement(1861..2940)
                     /locus_tag="VN647_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97209.1"
                     /translation="MKLKAGDWVEVKSKEEILATLDKNGRLEEMPFMPQMFEYCGQRF
                     KIYKRAHKTCDTVYPIRGRHLSDAVHLDVRCNGASYGGCEASCLIFWKTTWLKPVNAE
                     VTGTHPTSQVGRSISISSACTTEADVIAGTYVEDLGSPDGRRYICQATQLPRFTTDLN
                     WWDIRQYVEDLTSGNVTIHQFFKGVTYASYNNLINAGVGLGRALRWLYDRAQSVWGGV
                     PYPRKSGSIPINQSTPTCTLDLRPGELVRVKPFHEVLATLNTDNKNRGLYFDAEAVPY
                     CEGEYRVRSRLNKFIEEKTGKLITLKSSSILLEGVWCRSRYSECRMFCPRSIYTWWRE
                     IWLERVAEETESALENRPELPSSQS"
     gene            complement(2962..>3544)
                     /locus_tag="VN647_09020"
     CDS             complement(2962..>3544)
                     /locus_tag="VN647_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWG97210.1"
                     /translation="IEIGCGGTILRLLETHPGSRVVWVVLTTRGDRKKEAMKSAAVFL
                     RGAASTKLVTKSFRESYLPYQGKEVKEYFDTLPHVIDPDLIFTHYRHDFHQDHRVVCE
                     LTWNTFRNHQILEYEIPKYDGDLGQPNLYMPLTDEVSREKVRLLMEGFSTQRSKRWFT
                     EDTFYGLLRIRGIESPKSTKYAEAFYARKVVLE"
BASE COUNT          929 a          969 c          810 g          836 t
ORIGIN      
        1 cttgagtcct tttgcgcata gcactcagca atctcgagag acctatctgt gctgcaatta
       61 tttacaatgg tatagtccca gcgagggaat gtttgcgcca aaatactttc gatgcactcc
      121 gcaagatatt tctccccgtt ataaacggga gtaacaatgg aaaccaatgg atcatgcgca
      181 ttcatgccgc accattgttc gatagctatg ccaattcaag agattctttc cctccgaccg
      241 aagatcgctt tcttagacct ctaagcagat ccaagtaaaa tgacttctgt cccatgacta
      301 ccaagagcat ccaggaatat gaaaccatca aaatagttgc tcccaatatc agccgcggaa
      361 acggagacaa ggattggtca taaaaaaact gggcagcaga tgcgaatgcc gcggccacca
      421 ttgtcgaaag aaacggtcgc cccacagttt gtagaagatc tttccaagaa atcatcgtgc
      481 cgtgtataca ccaggcgata tgaggaacga cccacaacgt cagtacggcg gagtaggcga
      541 acgcgacgcc ttgcggaccg tacggtaacc caagaatgta cgctgtgatc accaagggag
      601 caatcaccag ggcgagcttc aagcttcgcc ccaccaatcc gattgagacc agcaaccagt
      661 acgtcggatt gatcagtgca aagatgagaa tcgtcggggt caataatctg aaaatcggca
      721 ccacatcttg ccactttggc cctaggacaa gaaaaatgat gtcatcggca aacaacgcgc
      781 acgcaacgga gattggtaag gtgagcgata aaactaatgc gtaacttttc aaaaagtagt
      841 ttttcaagcg ttcgggctca ttctggagtc gtgacaacgc cgcgaacaca accccgcctg
      901 ttgcggaatt caggttttcc gtggggatgc tcacaagctg gtaggctctt ccataatatc
      961 caagggtttc ggcaccccag aatcgaccaa gaagcacctt ttctaggtta tacgccacat
     1021 acacgacgag gctgtttagg gtggttgtgc ccccaaaacg caccatcgag ccgatttccg
     1081 ctcttcgatg cggcatcccc ggcacccatg taccgagtaa ccacaagcag atagtggaga
     1141 cagctggcag cgcgaccgcc atgccgacga gggcccaata tccgaacccg tttagagcca
     1201 tgccgatacc cacggcgata ctgatcaata acgcaaccgt ttcgatcacc gacaacgtga
     1261 caaagcgcat ttgcctctgg agaagcgccg agtgttgcac cccggccgca ttgaataaga
     1321 atccggctcc caacacggct gtcacataga aaagacgggg ttcttgatag aaagaaacga
     1381 gtatgggcgc gatcgcgagt gacagacacc ccagtattac acccaccgcc atattgagcc
     1441 agaataaggt cgacacttcc ttgtgggaga tcgtcgttcg ctgaactgtc gccatcgaca
     1501 ggccggcatc cctgaataaa ctaaacactc ctgtaacagc ggtgaccatc ccaaccaatc
     1561 cgaagtcctt cggatccagc aggcgcgcca ataccatcag cgagccaagc cgaagaaaga
     1621 agtttgctcc ttgggcgaag acttttgcgg caccgcctcg aagcgtcctc tctttgagat
     1681 cgttcattgg gaattggcgg acgagttcaa gttgccactg tctgattttt cgattcactc
     1741 agtgagtaag agaccggcaa tgggctctta ggcattcgca gaaacggtaa caaattaaag
     1801 gttgtccgct tccaacctat acgccctcac aatctcagac agggttgtcc tgtagtgtta
     1861 ctatgattgt gaggaaggca attccggcct attttctaat gcgctttcag tttcctccgc
     1921 aactcgttca agccagattt ctcgccacca tgtatagatg cttcgcgggc aaaacatccg
     1981 acactcactg taacgtgatc ggcaccagac gccttcgaga agaatcgaac tgctcttcag
     2041 cgtgatgagc ttgcccgtct tttcctctat gaacttattc aaacgcgacc gcactcgata
     2101 ttcgccctca caatagggta cggcttccgc atcaaaataa agccctctgt tcttattgtc
     2161 ggtattgaga gtagcgagaa cttcgtgaaa tggttttacc cggacaagtt cgcccggtcg
     2221 gagatctagg gtgcaagtcg gcgtagattg attaatcgga atgctgccgc tttttctcgg
     2281 atagggaaca cctccccata cagattgggc ccggtcatag agccaacgaa gagcacgacc
     2341 taacccaacc ccagcattaa ttaagttatt atatgaagca taggttaccc ctttgaaaaa
     2401 ttggtgtatc gttacattcc ccgaagtaag atcttccaca tactgccgga tgtcccacca
     2461 gttcaaatcc gtcgtgaaac gaggtaattg cgtcgcctga cagatatacc tcctcccatc
     2521 gggagaaccc aaatcttcta catatgttcc agcgataaca tccgcctcag tcgtacaggc
     2581 cgacgaaatg gatatcgatc gcccgacttg cgaagtagga tgcgtaccgg tcacttccgc
     2641 attaaccggt ttcagccacg tggtcttcca aaagatcaag caagacgcct cgcatccccc
     2701 ataggatgcc ccattgcacc tcacatctag gtggacggca tcggacaaat gtcttcctct
     2761 aatcggataa actgtgtcac aagttttatg agctctcttg taaatcttga acctctggcc
     2821 gcaatactca aacatctgcg gcataaaagg catctcttcc aaacgcccgt tcttgtcgag
     2881 cgtggcaaga atttcttcct tgctcttaac ctcaacccag tctccagctt tcaacttcat
     2941 ctgtccctca atgtttctag cttattcaag caccaccttc cttgcgtaga aggcctcggc
     3001 atacttggtt gacttgggag attcgatacc ccgaatccgg agaagtccgt agaaggtatc
     3061 ctccgtaaac caacgtttgc ttctttgtgt cgaaaatccc tccatgagca accgaacttt
     3121 ttcccggctg acttcgtccg tgagcggcat atacagattg ggttggccga gatctccgtc
     3181 gtactttgga atctcatact caaggatctg atgattgcgg aatgtattcc acgtcaattc
     3241 gcacacgacg cgatggtctt ggtggaaatc atgacgataa tgggtaaaaa tgaggtccgg
     3301 atcgatcaca tgaggaaggg tgtcgaaata ttcctttact tccttccctt gatacggcag
     3361 atagctttcc ctgaaagatt tggtgacgag tttcgtagac gctgctcccc ggagaaacac
     3421 ggccgcactt ttcattgctt ctttcttacg atctcctctc gtcgtcagaa ccacccatac
     3481 gacacggctt ccgggatggg tctccagcag cctgagaatc gtcccgccgc atccgatctc
     3541 aatg
//
LOCUS       DATNDD010000373         2472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_157987, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000373 DATNDD010000000
VERSION     DATNDD010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157987"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..672
                     /locus_tag="VN647_09025"
     CDS             97..672
                     /locus_tag="VN647_09025"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HWG97211.1"
                     /translation="MIDERDAIEKIDDPSGKEATPSSDSQYKVGPGRPPLESQFKKGA
                     PSPNPKGRPRKNASIAPDLKKLLEDGLKKKVKAKNGDNHVLLSRATLGIEQLLNQFAK
                     GDRYARRDLMDIAKELGVDLLAGQKEKIEEALAPSHQAILDSFISRHPQVRAAAPPPR
                     VIAPPELLDGIVPQVFSPEKVTRPLNRPPPK"
     gene            712..2157
                     /gene="terL"
                     /locus_tag="VN647_09030"
     CDS             712..2157
                     /gene="terL"
                     /locus_tag="VN647_09030"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HWG97212.1"
                     /translation="MTDQTTNTMAENVSPQAALRALLATDFASFIRYTFGVLRPGVEF
                     RPNWHIDAMAYKLSQIASGEVKKLIITVPPRNLKSICASVALPAWFLGHHPSERVVAV
                     SYSSELAKTHASDFRRVVTDPLYQAIFPGMRIQRETDKEIATTARGRRYATSIEGTLT
                     GLGGNLIVIDDPIKLGDASSEVVRKRSIDWYRNTLVTRPDDKKAARIVVVMQRVHQED
                     LVGYLQEQGGFDILNLPAIAQTSSTFSTGSRSSHMRLAGEILHPEHESGEILLGIKRD
                     MGSMQFSAQYQQTPVPAGGTFIKRTWLKTYPASEISPQHRDRMVISWDIALSEAQTGD
                     SSAGVVLLCRGDTFYVLEVCKGQFPFGTLIEKILDMVKRYHPNSSLVIEESPISLGLI
                     QALKQNHINVVAITPTKDKRSRVISQTDRFEGGSVLLPERAEWLDGFIGELLSFPGGR
                     HDDQVDALVQGMAWMRAQWMPPVKSRIVLNW"
BASE COUNT          600 a          708 c          652 g          512 t
ORIGIN      
        1 tcggaccttc gaagacatgg agagcgaacg cacctccatc gcaacggaga ctcctgcaga
       61 atgcaattcg gctcctgcgt ccgacgaggt gcggccatga tcgacgagcg cgacgccatc
      121 gagaagatcg atgatccatc cgggaaggag gccaccccat catctgacag ccaatacaag
      181 gtcggtccgg gtcgccctcc cctggaaagc caattcaaga aaggggctcc atctccaaac
      241 ccaaagggcc gccctcgaaa gaatgccagc atcgctcctg acctgaagaa gctactggaa
      301 gacgggctca aaaagaaggt taaggccaaa aacggcgata accacgtgtt gttgtcaaga
      361 gccactcttg gcatcgagca actgctcaac caattcgcga aaggcgatcg ctacgcccgg
      421 cgcgatctta tggatatcgc caaggaacta ggtgtcgatc tgctagcggg gcaaaaagag
      481 aaaatcgaag aagcgttggc gcccagtcac caagctatcc tagacagttt catctcgcgt
      541 cacccacaag tgagagctgc ggctccgcct ccgagggtta tagccccacc cgaattgctc
      601 gacggaatcg taccccaagt cttttctcct gaaaaagtca caagaccact taatcgccct
      661 cctcccaaat aaataccaat tgcagggaga cggacctgga aaagtcgtac catgactgat
      721 cagacaacga acactatggc ggagaatgtt tcgccgcagg cggcgctgcg tgcgctgctc
      781 gcgactgatt ttgcatcgtt tattcggtac accttcggtg tgcttcggcc cggcgtcgaa
      841 tttcgaccga actggcacat cgatgccatg gcctacaaac tgtcccagat tgcaagcggt
      901 gaggtgaaaa aactcatcat caccgttcca ccccgcaacc tcaaatcaat atgcgcctcg
      961 gttgcgttgc ccgcctggtt tcttggtcac cacccctctg agcgggtcgt tgctgtttcc
     1021 tattcatcgg aactggccaa aacgcatgcc agcgacttcc gccgggtcgt gaccgatccg
     1081 ttgtatcagg ccattttccc cggcatgcgc atccagcgcg aaaccgacaa ggagatcgca
     1141 acgacggctc gcggcagacg ttatgcaacc tcgatcgagg gaacgttgac cggtcttggc
     1201 ggcaatctca tcgtcattga tgatcccatc aaacttgggg atgcctcttc cgaggttgta
     1261 cgcaaacgca gcatcgattg gtacagaaac acccttgtca cccggccgga cgacaagaag
     1321 gccgctcgca tcgtcgtcgt catgcagcgt gttcatcaag aggacctcgt cgggtatttg
     1381 caagaacagg gtgggttcga catcctcaac cttccggcta ttgcccagac gagctcaact
     1441 ttttcaactg gctcgcgaag ctctcatatg cggcttgcag gagagattct tcaccccgag
     1501 catgagtcag gtgaaattct gctcggaatc aaacgagata tgggctcgat gcagttctcc
     1561 gcccaatatc agcagacacc ggtgccggcg ggagggacat tcatcaagcg aacgtggctg
     1621 aagacctatc cagcatcgga aatatcaccc cagcaccgag atcgcatggt cattagctgg
     1681 gatatcgctt tgagtgaggc ccagaccggt gacagttcgg ccggcgttgt gctgctatgt
     1741 cgcggagaca cgttctatgt tctcgaggtc tgcaaggggc agtttccgtt cggaacgctt
     1801 attgaaaaga tcctcgatat ggtcaaaagg tatcatccaa attcctcctt ggtgatcgag
     1861 gaatctccga tcagcctcgg tcttatccag gctctgaagc aaaaccacat aaacgtggtc
     1921 gcgatcacac cgaccaagga caagaggtcg cgcgttattt cgcaaaccga ccgtttcgaa
     1981 gggggctccg tgctcttgcc agaacgggcc gagtggcttg acggcttcat cggcgaattg
     2041 ttgtcgttcc cgggcggccg tcacgacgat caggtcgatg ccctcgttca aggcatggcc
     2101 tggatgcgcg cgcagtggat gccgcccgtc aagagccgaa ttgtgctgaa ttggtaacaa
     2161 ctacctctgc cctccatggt taccaacatt gggactcatc aaaatgaaat tcaggaagcc
     2221 actcaagatt accagccgga cgtcatcgat caccaatgca tttgttcagg caatcatacc
     2281 gagtgtagag ccttcggccg aggaagaagc tgaggcactg tcaattcttg ggatgacgct
     2341 ggagacaata caatgcgtat attgcggcga ccctgcgacg gactgggatc acctacggtc
     2401 ctacgtccga aagaaagagc cgtctggcta cgtcaatgat gtcaggaata tcgttccttc
     2461 ctgcggacca tg
//
LOCUS       DATNDD010000374         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_798291, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000374 DATNDD010000000
VERSION     DATNDD010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798291"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..520)
                     /locus_tag="VN647_09035"
     CDS             complement(182..520)
                     /locus_tag="VN647_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWG97213.1"
                     /translation="MACKPGDLLLIPFPYSDLQSTKKRPVMVLTPPDRHEDFIGLAIT
                     SVEHKQAALQIGTANLIHGTLPKPCRVRFDKVFTLSESSIVKTFGGVSPQTIQAVLQG
                     LCSVVGYART"
     gene            complement(510..710)
                     /locus_tag="VN647_09040"
     CDS             complement(510..710)
                     /locus_tag="VN647_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HWG97214.1"
                     /translation="MSTAERIFREVQKLPDTLAQEVLDFVEYIELKHGFRDRSAEELK
                     QAQEPAMHHVWDNQEDEVWDGV"
     gene            complement(879..1754)
                     /locus_tag="VN647_09045"
     CDS             complement(879..1754)
                     /locus_tag="VN647_09045"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HWG97215.1"
                     /translation="MSDWTTGYKKGRRRLYSWSLCLIVLLALTGCSFTMTYRYADRLI
                     IWKLDYYFDLNRDQRHDLTQRLTPILDKHRQEALPQYESFLREIRQKVERGLTGSDID
                     WAYATYDHLRTDLFERVVADSGAFLASVDARQVLRFESALQKDNTKATRLVQTPAPER
                     LKERAQATLDLTQDWLGRLTKEQEVQIREWSFMLPDVEPMWVAYQQQRRQELVALLHQ
                     PRTPEQIARELRSMFVYHDQTAPQSYQHAIRQIRENAKTVALAIDQWMTPEQRRHAVT
                     KLQRLIDQVHDLQAG"
BASE COUNT          444 a          545 c          626 g          483 t
ORIGIN      
        1 taccgctgcg cgctcacccg cccgcggtga gcctgtcgaa ctgcccgccc tgagcttgtc
       61 gaagggcctc ccctgcccat ggcgagtttg gtcgggtcga gacgggtgag aggtgggctc
      121 cttgaggagg ctgcgctatt cctaagcgaa gcgttttgct ttcaactgat gggctttggg
      181 ctcaggtacg agcataccca acgacagagc agagtccttg taacacggct tgaatggtct
      241 gaggtgatac tccgccgaag gttttcacaa tgctgctctc ggaaagagta aacactttgt
      301 cgaatctgac ccgacagggt tttggaagcg ttccgtgaat caaattcgcc gtcccaattt
      361 gtaacgccgc ttgtttatgt tcaaccgagg tgatggccaa tccgataaag tcttcgtgtc
      421 gatctggagg agtcaagacc atcacgggac gcttcttagt agactggaga tccgagtagg
      481 gaaagggaat cagaaggaga tcaccgggct tacacgccat cccaaacctc atcctcctgg
      541 ttgtcccaca catggtgcat cgccggttct tgggcctgtt tcagttcttc ggccgatcgg
      601 tcgcggaatc cgtgcttgag ttcaatgtac tcgacaaaat ccaacacctc ctgcgcaagc
      661 gtgtcgggaa gcttctgcac ctctctgaaa atgcgttcgg ctgtcgacat atcgtccctt
      721 tcttctccta ggcgagctga ggcttcctcg gtgttctatt atcgtgagcc tagtgtagtc
      781 aaagcccgcc gagaattcca gcaatgtgcc ttctcgggct aggaattggc tgctcagcta
      841 tcgggacgac tcaaggtgcg ccaaataaag ccggaagcct acccagcctg gaggtcatgg
      901 acctgatcaa taagccgttg gagtttggta acggcgtgcc ggcgctgttc gggagtcatc
      961 cattgatcga tggccaaggc cacggtcttg gcattctcgc gtatctggcg gatggcgtgt
     1021 tgataggact gcggagcggt ctgatcgtgg tacacgaaca tagagcggag ctctcgggcg
     1081 atctgttcgg gagtccgggg ctgatgcagg agggccacga gctcctgccg gcgttgctgt
     1141 tgatacgcga cccacatcgg ttccacatca ggcagcatga aactccactc tcgaatctgg
     1201 acttcttgtt ctttggtcag tcgtcccagc cagtcttgtg tcaaatcgag ggtggcctga
     1261 gccctttctt tgagacgttc cggtgcagga gtctgaacga ggcgcgtcgc tttggtattg
     1321 tccttctgca aggccgattc gaaccggagc acctgccggg catcgaccga ggcgagaaag
     1381 gccccgctgt cggctacgac acgttcaaag aggtccgtgc gcagatggtc gtaggtggca
     1441 taggcccagt cgatatcgga accggtgagt cctcgttcga ccttctgacg gatctcccgg
     1501 aggaaggatt catactgtgg caaggcctcc tgccgatgtt tatcgagaat gggcgtgagg
     1561 cgttgtgtca gatcgtgccg ctgatcacgg ttgaggtcga aataatagtc gagcttccag
     1621 atgatgagtc ggtcggcata ccgatacgtc atggtgaatg agcagcctgt gagggccaat
     1681 agcacaatga ggcacaacga ccaactatac agtcgtctcc ggcccttctt gtaccctgtc
     1741 gtccagtcac tcatgatccg tgcagatatg ttctcaagca aagcggaatg atgcaagggt
     1801 cgacctaaca gcgtggaatg gaatgagatg tggacgtgag agattgtggc tagctctggc
     1861 cactaagccg aacacgtgga ctttccgtgc gccatgacgt gcggcgatct ggagaaccct
     1921 gactcggtta gaccgcaata gcgactggat acttatgcgc gcagtctagc caattcatgg
     1981 gtagaactcc agcctgagag gaaaatggaa accggtcctt gaagaaggaa tgagagaccg
     2041 gtcgtgatga gaccgcttag gtcggaatgt cgcgggtccc gtcgtaggat gtgcggga
//
LOCUS       DATNDD010000375         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_12881, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000375 DATNDD010000000
VERSION     DATNDD010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12881"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /gene="pyrH"
                     /locus_tag="VN647_09050"
     CDS             <1..601
                     /gene="pyrH"
                     /locus_tag="VN647_09050"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWG97216.1"
                     /translation="ALDIQVAVVIGGGNIFRGIAASASGMERASADYMGMLATVLNAL
                     ALQNALERIGIMTRVQSAIEMRQLAEGYIRRRAIRHLEKRRVVIFAAGTGNPYFSTDT
                     AAVLRAMEISAQVIMKGTKVDGIYDADPVTNPSAKKYERISFLSILNQKLKVMDSTAI
                     SLCMDNKLPLIVFNLKVKGNFKRVALGEPIGTLVTLGDR"
     gene            622..1185
                     /gene="frr"
                     /locus_tag="VN647_09055"
     CDS             622..1185
                     /gene="frr"
                     /locus_tag="VN647_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWG97217.1"
                     /translation="MSNAASVRQAFITHMDQSLEHLRKDLSGLRTGRASVALLDGIRV
                     EYYGTMTPLKQIANVSTPEARLITIQPWEPKLIKEIEKAISNSGLGVTPSNDGKVIRV
                     PLPPLTEERRKELTKICKKHGEETKVQIRGFRRDANEELKKLQKDAKLTEDELRKAEQ
                     ETQKLIEQYGQKIDDVIKKKEQEIMEV"
     gene            1215..2348
                     /gene="alr"
                     /locus_tag="VN647_09060"
     CDS             1215..2348
                     /gene="alr"
                     /locus_tag="VN647_09060"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWG97218.1"
                     /translation="MSITPTFLPTVAIVDLTALVHNLSQFRRMLSPGCDVIAVVKANA
                     YGHGAIETSRTLIRHGVTRLAVFSTEEGVALRQADITVPIVILGPIFQEQFGDLFAHQ
                     LTPVVSDPSVLMALGQAAASREVSHSIHLKIETGMGRLGLTQDELVALIRSYKFPSSL
                     RLEGLMTHLADADGSDPDATEEQISRFHLALNVVLEGGFQVPLIHMCNSGGAVRFPSA
                     HFSLVRPGIMLYGYHTLPSTVETPDLRPVLSLKTRIAQLRTIQPGGTVSYNRTFTAKC
                     PTRIAILPIGYASGLSRHLSNRGYVLIRGQRVPIAGLVCMDMVMVDVTTIPGIAVGDE
                     VVLIGQQENERITARDIAEWTGTISYEVLCAISPQIPRIYYSS"
     gene            complement(2419..3858)
                     /locus_tag="VN647_09065"
     CDS             complement(2419..3858)
                     /locus_tag="VN647_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWG97219.1"
                     /translation="MRALSHWGMPKGGRWYSFRYRVRVCVRQGFALLALAVIVCTVSP
                     VSAQVPRVYGQGAAASGMGNAFAAQADNPSALHYNPAGMTQLRGIQIMGGGTLVGGTT
                     DFRSPSGISATGDHDGAFAWPGPGHGYITANLHGLGVSALEKLTVGIGVTTPFGSVMR
                     WPDNSPFRGVTTFTALPLFDIKPTLAYQLHPDLSIGAGADIYTFANFFGEGHAELQSI
                     SPGGLAPAGTKLEFNGKGTAPGFNVGALYTALRNGEGQPVANIAVVYRSQATLHLDGA
                     LLANGVKIQDATTTLVLPQVITGGIALWPVRNTEREWKVELNVDYVGWKSVRNLDIHL
                     ANGAVIPQPQNWHGTYAILVGTEYRWLRVDRLPGWEIALRGGYTNQQAQVPDLNFNPA
                     VPSADLHVISTGIGLLCKSNGSFLGLISCGSIGLGPVKAKLVGVDLSYQAFLYEPRTI
                     SGNTGLRAVVNGLYDSTLHAGTFSVRASF"
     gene            complement(3872..>4186)
                     /locus_tag="VN647_09070"
     CDS             complement(3872..>4186)
                     /locus_tag="VN647_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97220.1"
                     /translation="LHDRLKAEGYQTFSAFDGRQALATLQSREFDGMILDIGIGQIDG
                     LEVLRRVRLTNQRLPIIMITASGSQELAVRAIGLGAQAYLLKPFDAGQLQQTMDRWFR
                     RA"
BASE COUNT          974 a         1222 c         1107 g          883 t
ORIGIN      
        1 cgcccttgac atccaagtgg cggtcgtcat cggcggaggc aatattttcc gtggaattgc
       61 ggcaagtgcg tccggtatgg aacgggcttc ggcagattac atggggatgc tggcgacagt
      121 gctcaatgcg ctggctctcc aaaatgcgct ggaacggatc ggcatcatga cccgtgtcca
      181 atctgccatc gaaatgcgcc agctcgcgga ggggtacatc cgtcggagag cgatccgcca
      241 ccttgaaaag cgccgcgtgg tcatttttgc cgccggcacg ggcaatcctt atttctcgac
      301 cgatacggcc gccgtcctac gggccatgga gatcagtgct caagtaatca tgaaagggac
      361 gaaagtcgac ggcatctacg atgccgaccc cgttaccaac ccgtcggcga agaagtatga
      421 gaggatttca tttctgtcca tcctcaacca gaaactcaag gtgatggatt ccacggcgat
      481 cagcctctgt atggataata aattgcctct catcgtgttc aatctgaaag tcaagggtaa
      541 ctttaaacgc gtggcgttag gcgagccgat cgggaccttg gtgacgctcg gcgatcgctg
      601 atcccatcac gaggtgtctt catgtccaac gcagcctccg ttcgacaggc attcatcacc
      661 cacatggatc aatcgcttga acacttgcgg aaggacctgt ccggcctccg aacagggcgg
      721 gcctccgtcg cactgctcga cggtatccgt gtcgaatatt atggaaccat gactccgctc
      781 aaacagatcg ccaacgtctc cacccccgaa gcacgactca ttaccattca accctgggaa
      841 ccgaaactga tcaaagaaat agagaaagcc atatccaatt cagggctggg tgtgacgcct
      901 tcaaacgacg gcaaagtgat tcgagtcccc cttccccctc tgacggaaga gcgccgcaag
      961 gagttgacga agatctgcaa aaaacatggg gaagagacga aggtccagat tcgaggtttt
     1021 cggcgagacg cgaacgaaga gttaaagaag cttcaaaaag acgcaaagct caccgaggac
     1081 gaactgcgca aggccgagca agagactcag aagttgatcg agcagtacgg tcagaagatc
     1141 gatgacgtga tcaagaaaaa ggaacaggaa atcatggagg tctgagtctg acctcctgat
     1201 gttcgtttct tcgggtgtcg attacaccaa cattcctccc gaccgtcgcc atcgtagatc
     1261 tgacggcgct cgtacacaat ctctcccaat ttcgtcggat gctctccccg gggtgcgacg
     1321 tcatagcggt cgtcaaagcc aatgcctacg gccacggcgc gatcgaaacg tcacgaacac
     1381 tgatacgaca tggtgtcact cgccttgccg tcttttcgac ggaagaagga gttgcacttc
     1441 gacaagccga catcactgtg ccgatcgtca tcctaggacc gatctttcaa gaacaattcg
     1501 gcgatctgtt tgcccaccaa ctcactccgg tagtgagcga tccttccgtg cttatggcgc
     1561 tgggacaagc cgcagcatca cgcgaagtct cccactcgat ccacctcaaa atcgagacag
     1621 gtatgggtcg gctgggcttg acgcaggacg agctggtggc gctcatccgc tcatacaagt
     1681 ttccttcctc cttacgattg gaaggactca tgacccatct ggccgatgcc gatggatccg
     1741 acccggacgc gaccgaggaa caaatcagtc gattccatct agccctgaac gtcgttctgg
     1801 aaggtggatt ccaggttcct ctcattcata tgtgcaacag cggtggagca gttcgcttcc
     1861 catcagccca cttttccctc gtgcggcccg gcatcatgtt gtatgggtac cacacccttc
     1921 ccagtacggt ggagactcca gacctaaggc cggtgctctc actgaagacc cgtatcgctc
     1981 aactccgaac tatccaacca gggggaacgg tcagttataa tcggaccttc accgcaaagt
     2041 gccctacacg gattgcgatt ctcccgatcg gttatgcaag cgggttgagc cgacacctct
     2101 ccaatcgagg ctatgttctc attcgcggac agcgagttcc tatcgcggga ttggtgtgca
     2161 tggacatggt gatggtggat gtcaccacga taccgggcat agcagtcggg gacgaagtcg
     2221 tgctgattgg gcaacaggag aacgaacgaa tcacagcccg cgacattgcc gagtggaccg
     2281 ggacgatttc ctacgaagtt ctctgtgcga tcagtccgca gattcccagg atctactact
     2341 cctcctaatc cgtcccacac agccgttcgc cggtgtccgc agagaacgga gcaaaccgat
     2401 gtatgccata aaccctatct agaaactggc tcggacggaa aacgtgcccg cgtgcaaggt
     2461 gctatcgtag agtccattca ctactgcacg aagcccggta tttcctgaga ttgttcgtgg
     2521 ctcgtagagg aacgcctggt acgataaatc gacgccgacc agttttgcct tgaccggtcc
     2581 gagtcctatg ctaccgcagg agataagtcc caagaatgac ccattgctct tgcacaacag
     2641 tccaatgcct gttgaaatga catgcagatc ggccgatgga accgctggat tgaaattgag
     2701 atcgggcacc tgcgcttgct ggttggtata gccgcctcgt agagcgatct cccatccagg
     2761 caaccgatcc actcttaacc atcgatactc ggtcccgacc agaatggcat aggtgccgtg
     2821 ccaattttgc ggctggggga tgaccgctcc gttggccaga tggatatcca gatttcggac
     2881 cgatttccaa cccacgtaat ctacgttcaa ttccactttc cactcgcgtt cggtgttccg
     2941 gaccggccaa agcgcaattc cgcctgtaat gacctgaggc aacaccaacg tcgtcgtcgc
     3001 atcttggatc ttcacgccgt tggccaacaa ggctccgtcc aggtggagtg tcgcttggct
     3061 tctgtatacc acggcgatat tggccaccgg ttgcccctct ccattccgga gtgccgtata
     3121 caatgctccg acgttgaatc ctggagctgt gcctttgccg ttaaattcga gtttggttcc
     3181 ggcaggcgct aatccgccgg gcgaaatcga ctgcagctcc gcgtgccctt caccgaagaa
     3241 attcgcgaag gtatagatgt ctgcccccgc accgatcgag agatccgggt gaagctgata
     3301 ggcaagcgtg ggtttgatat cgaataacgg caaggcggtg aacgttgtga cacctcgaaa
     3361 cggactgtta tccggccatc tcatcaccga gccgaatgga gttgtgacgc cgatccctac
     3421 cgtcaacttc tctagtgccg agacacccag gccgtgaaga ttcgccgtaa tatacccatg
     3481 accaggacca ggccacgcga acgcgccatc atgatcgccg gttgccgaaa tcccgctcgg
     3541 acttctaaaa tcagtggtcc ctccgacgag agttcccccg cccattattt gtattccacg
     3601 cagctgggtc atgccggctg ggttataatg aagagccgaa ggattgtctg cctgtgcggc
     3661 gaaggcatta cccatcccgg acgcagcggc tccctgacca tagactcgag gcacttgagc
     3721 cgaaacaggg gaaaccgtgc agacgatgac ggctagtgca agaagagcaa acccttggcg
     3781 cacacaaacc cgaaccctat accgaaagga ataccatcgc cctcccttgg gcattcccca
     3841 atggctcagc gcgcgcatct catcctcctg gtcatgcacg ccggaaccac cgatccatgg
     3901 tttgctgaag ctggcccgca tcgaatggct tgagcaggta ggcttgggct cccaacccga
     3961 tggcccttac ggcgagttcc tgggatcccg atgccgtaat catgataata gggaggcgtt
     4021 gattcgtcag acgcactcgt cgcagcactt ccagaccgtc gatttgaccg attccgatgt
     4081 cgagaatcat cccatcgaac tcccgagatt ggagagtcgc cagtgcttga cggccatcga
     4141 acgccgaaaa cgtctggtag ccttctgcct tcagccggtc atgaag
//
LOCUS       DATNDD010000376         3253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3896232, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000376 DATNDD010000000
VERSION     DATNDD010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3896232"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VN647_09075"
     CDS             <1..888
                     /locus_tag="VN647_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG97221.1"
                     /translation="TDARTLEPIILKLAELLQAPVPRAAAPSPTNDIGTPAPRPPAAG
                     PSEPIIRKQERQAAIVEFSGFLATEIREPLASIRSQLEEVTGEGILDFDPQTRVENAM
                     RDLIRIEAILNETLDFAKPLELNRHLCRIPEVLESALVVVATDLEATRIQVSKDYANV
                     IAPVRGDEAKLQQAFLSIFRNACEAMSPGGHLHIQVTQHRAGRGLEVQILIQNDGVPI
                     PAEIVDKVFEPFFTTKQSGTGLGLSSVKKIVEEHGGTIAIGSAPGEGTTVTIRLPGIS
                     RGPAFRHRGRGRRHPRRPN"
     gene            1099..1464
                     /locus_tag="VN647_09080"
     CDS             1099..1464
                     /locus_tag="VN647_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97222.1"
                     /translation="MKLLTGAVATVSSILLACSIASANPALLPKHDGYPMKNDGSPVN
                     GQPTANDPGQKDAHGASTLLKSAESVQNAEQKLIKTDNARITKEQGAGRLPNVEGPQI
                     EIAPPVTSATKITGDRQID"
     gene            complement(1550..2089)
                     /locus_tag="VN647_09085"
     CDS             complement(1550..2089)
                     /locus_tag="VN647_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWG97223.1"
                     /translation="MADNILPKRYFNRPTLTVARSLVGKYLVRENGKGTIAGRIVEVE
                     AYVGCEDKACHASRGRTARTEVLFGPPGMSYVYLIYGMYHMLNVVTERMEFPAAVLIR
                     AIEVDGELIDGPGKLCRALEIDRSLNQLDMTNGRSLWFEDRGARVASKQVGMFPRIGV
                     DYAGVWAKKPWRFRLGGIG"
     gene            complement(2108..3085)
                     /locus_tag="VN647_09090"
     CDS             complement(2108..3085)
                     /locus_tag="VN647_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HWG97224.1"
                     /translation="MKPNSISQFIDHHYRHFNAAAMKDAAHAYRAHLDKGGKMLVTLA
                     GAMSTGELGLSLAEMIRRDNVHAICCTGANLEEDLFNLVAQTHYVRIPSYRELTAQDE
                     RVLLERHLNRVTDTCIPEAEAMRRIEAAVASEWVAADRSGVREFPHEFLYRLLRDGKL
                     KEYYQIDPDDSWMYAAAERNLPVFVPGWEDSTLGNMYAAHCLTGTIKNVHTVRTGIEY
                     MMHLAEWYPKVTEANSLGFFQIGGGIAGDFPICVVPMLSQDLRKEQVPFWGYFCQISD
                     STTSYGSYSGAVPNEKITWGKLGVETPKFIIESDASIVAPLIFAYILGW"
BASE COUNT          767 a         1009 c          769 g          708 t
ORIGIN      
        1 accgatgccc ggacgctgga gcccattatt ctcaaactcg cggagctctt acaggcaccc
       61 gtgccgcggg ctgccgcccc ttctccgacg aacgacatcg gaacaccggc cccccgaccg
      121 cccgctgccg gcccgtccga gcctattatt cgaaagcaag aacgccaggc cgcgattgtg
      181 gagttcagcg ggtttttggc gacggagatt cgagaaccgc tcgcatccat ccgctcgcaa
      241 ctcgaagaag tgaccggtga aggaatcctc gacttcgatc cccagacccg tgtcgaaaat
      301 gcgatgcgcg acttaattcg gatcgaagcg atcctgaacg agaccctcga cttcgccaaa
      361 ccgctcgaac tgaatcgcca tctgtgccgt attccggagg tgcttgagag cgctctcgtg
      421 gtggtcgcaa ccgacctgga agcgacccgt atccaagtct ccaaagacta cgccaatgtc
      481 atcgctcccg tgcgcggcga tgaggccaag cttcaacagg cgttcttgag tatcttccgg
      541 aacgcgtgcg aggccatgtc tcccggcggc catctccata tccaggtcac gcaacatcga
      601 gccggacgag ggctcgaagt tcaaattctc attcagaatg atggagtccc catcccggct
      661 gaaatcgtcg acaaagtttt tgagccattt tttacgacca aacaatccgg caccgggttg
      721 ggtctctcca gcgtcaaaaa aatcgttgag gaacacgggg gaaccattgc catcggcagt
      781 gccccaggtg aaggcaccac cgtcaccatc cgtctccccg gcatcagccg cgggccggca
      841 tttcggcatc gcggccgtgg ccgtcgccat ccacggcgac cgaactgata tgtccgtctt
      901 tatcttgccc acattcctcg catgatctac ggctcgtcac gacatgacgg gaatcaattg
      961 ataacgtccg gcctgtcttg cccgtttgac agaaatgacc ctccatcgct atgattcagc
     1021 ccgtgcggca aatccttcga tttgaatgtt cagaatacat gattctcatt cattcataat
     1081 cttaaaggga gaacgagcat gaaacttctt accggtgcag tcgcaacagt ctcctcgatc
     1141 ctgcttgcgt gctcgatagc ttcggccaat ccggcgctgc tgcccaaaca tgacggctac
     1201 cccatgaaga acgatggcag tcccgtgaac ggccaaccga cggcgaatga tcccggccaa
     1261 aaagatgcac acggagcgtc cacgttgctg aagtcggcgg agtccgtcca gaacgccgaa
     1321 cagaaattga taaaaaccga caatgcacga atcacgaaag agcagggtgc cggaaggctt
     1381 cctaatgtgg agggacctca gattgaaatc gctccgccgg taacctctgc gaccaagatc
     1441 accggcgatc gccagatcga ctaattcgtc catactgaaa gggggaaatc tctccaaggt
     1501 ttcccccatc agccgccctg tttcttcctt acccaacaaa gggtatcttt tagccaatcc
     1561 cgcccaatct aaaccgccaa ggttttttcg cccatacccc cgcatagtcc acaccgattc
     1621 tcgggaacat gccgacctgc ttgctggcaa cccgtgcgcc tcgatcttcg aaccagagag
     1681 atcgtccgtt cgtcatgtcg agttgattta aggaccgatc gatctctaat gcgcggcaga
     1741 gctttcccgg cccgtcaatc aactcaccgt cgacttcaat cgctcgaatg agtaccgctg
     1801 caggaaactc catccgctcc gtcaccacat tcaacatgtg gtacatgccg taaatgaggt
     1861 agacgtatga cattccagga ggaccgaaca acacctccgt ccttgccgtc cttcctctcg
     1921 aagcatggca cgctttgtcc tcacaaccga catacgcttc tacctcaacg atccttccgg
     1981 caatcgtccc ttttccattt tcacgcacca gatacttgcc gaccaaggac cgcgctacgg
     2041 tcaatgtagg gcggttgaag tatctcttcg ggaggatatt gtcagccatt ccgattgttt
     2101 caattctcta ccagccgagg atatacgcga agatcaacgg cgcaacgatc gaggcatcgg
     2161 attcaatgat gaatttcggc gtctccacac ctaatttccc ccacgtaatt ttttcgttcg
     2221 ggacggcccc ggaataggat ccgtaactgg tggtcgaatc gctgatttga caaaagtatc
     2281 cccagaaagg gacctgctct tttcgaaggt cctgactcag catcggaacc acacagatgg
     2341 ggaaatcacc ggcaatcccc cctccaatct ggaaaaatcc gagcgaattc gcttccgtga
     2401 ctttcggata ccactcggcc aaatgcatca tgtattcgat gccggttcgc accgtatgca
     2461 cgttcttgat ggttcccgtc agacagtgcg ccgcatacat attccccagc gtggaatcct
     2521 cccatccggg gacaaacacc ggaaggttcc gttccgccgc cgcgtacatc caactgtcgt
     2581 caggatcaat ttggtaatat tctttgagct tcccatcccg caagagccga tacaaaaact
     2641 catgggggaa ttctcgaacg ccactgcggt cggccgccac ccattcggat gcgactgcgg
     2701 cttcaatgcg gcgcatcgcc tcggcttcgg gaatacacgt gtcggtcaca cgattcaagt
     2761 gtcgctcgag caaaacccgc tcatcctgcg cggtcaactc gcgatacgac ggaatccgca
     2821 cgtaatgcgt ttgcgccacg aggttaaaga gatcttcctc aagattggcg cctgtgcagc
     2881 aaatggcatg aacgttgtcc ctgcgaatca tttccgccaa cgacaacccc agttcgcccg
     2941 tgctcatcgc accggccagg gtcaccagca ttttcccacc cttgtccaag tgagccctgt
     3001 aagcatgcgc cgcgtctttc atcgccgccg cattaaaatg tcgataatga tggtcaatga
     3061 attgcgaaat ggaattcggc ttcatatgag actccttgat tttgaaactg tcatcagtta
     3121 ttacaaccat cccccacacc gtcctctact cttcactaaa agaacagtat accctgatcg
     3181 tctccttaaa aatgtaaggc gacccctccc ataaatgtgc gcgggttacc cggcacaaaa
     3241 tgaatatcgg tga
//
LOCUS       DATNDD010000377         5463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3604290, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000377 DATNDD010000000
VERSION     DATNDD010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3604290"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..420
                     /locus_tag="VN647_09095"
     CDS             127..420
                     /locus_tag="VN647_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97225.1"
                     /translation="MDRSMIVTAWEQHCADGWPQFSSPDQGQLMTIDTVISGCVVYFL
                     DSSEGLDDQRVAIVKDCLGDLDELTEALDTGSQAYFYRLRKLGAMLLGDEPQS"
     gene            572..1102
                     /locus_tag="VN647_09100"
     CDS             572..1102
                     /locus_tag="VN647_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97226.1"
                     /translation="MTNARVIRMAIILLASILATVGSAHAEPYELSKNDVMEPKAISS
                     AEISLFGVKLGDSESKAVETLVNEKISGIKAEQEATFIFLLDQRKPIGPMAGVRIQDG
                     KVDLLFINNRFAYKTRGIFRNVLNSESPEDIRKLLGQEEYGDENVMGAVMAYDKQGFQ
                     VNYLGKDVNIEFAAPR"
     gene            complement(1265..2197)
                     /locus_tag="VN647_09105"
     CDS             complement(1265..2197)
                     /locus_tag="VN647_09105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWG97227.1"
                     /translation="MAQKDHIRPDFRLQNLGRTLCRFGLFGSLFLSMIGLSILPASAM
                     EANFKDRAVHTISYVPQPLPWKRIPAHSILLKELRSGRILFEHEVEKRLSPASLTKIM
                     SALIILEKGRLDDLATVSRNAARAPKTHLRLKVGEVFRLGDLLKAMMMVSANDACLAA
                     VEHVGGDEEQFVTLMNAKALALGLSDTHFSNGCGFDGPDHYSTAENLATLSEVAMRNA
                     VFRELVKGEREIITPVSGYRAYVLHNTNRLLGRIPGVEGVKTGFTSKAGRCLIAKVSH
                     NGSDLLLVILNSNRRWNTAKSLIDYGFRLTTTVQ"
     gene            complement(2420..2626)
                     /locus_tag="VN647_09110"
     CDS             complement(2420..2626)
                     /locus_tag="VN647_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97228.1"
                     /translation="MALRSFAWWFMIGALMTLSVLMVQGGIRDLWEGTQPAGGTNVFV
                     YFGTTLFGGGLLAGCVALIMNRLR"
     gene            complement(2720..3916)
                     /locus_tag="VN647_09115"
     CDS             complement(2720..3916)
                     /locus_tag="VN647_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWG97229.1"
                     /translation="MNIHIERTSHAARALFSLLWLALLVSHGLWACSKSEPYNPPDPF
                     YYFASYKVGKNPTTVITEDLNHDSFTDLVTTNIASNTLSILLGNGDGTFSDQVQLHVC
                     QEPRSLVMGNFNQDRHADVALACSGGDEVMVLLGHGDGRFEEGPRYPVHRAPIALAAD
                     DINGDHHTDLVVALRNDKVKVFLGNGTGEFQHGAQYEHGDTPTSIALSDLNADGKLDL
                     VVTNGGPMSNAVSIWLGNGDGSFHDPKDYSTGHRPLGVSFADFNNDHHRDLLVINGEQ
                     DSFTTFLGNGNATFRPGKHSGADAGPNFGLARDFNGDRLEDVAIVNLQSNDLSILFGK
                     GDGTFHYPPRNYRTKSGPFALSSFRVTTSGLEEPGLAIADNGSGSVSIFLHHGLKPVA
                     KTETRQ"
     gene            4089..4409
                     /locus_tag="VN647_09120"
     CDS             4089..4409
                     /locus_tag="VN647_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97230.1"
                     /translation="MSKIVNVDITMYGIAEVLMWCLDRNKGRVPGVDTPGFKKMQELL
                     AQKPQSADYFTLDQFWKKKVTLPLTEDEVATIDRCLYDIPNFDNEPLPQIRHKFWPQQ
                     VETH"
     gene            complement(4485..>5463)
                     /locus_tag="VN647_09125"
     CDS             complement(4485..>5463)
                     /locus_tag="VN647_09125"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HWG97231.1"
                     /translation="ANTTEIHWTDGALKRMERAPIFLRGMVRRLAEKKARELGYEEIT
                     EEILEQFKGQMMGRMGGEAGMASAVEEMADGRLPWTAAAKERLNTVPEFMRPMTKQIA
                     EEIAKERGHLEVNVELFEKVEALGDLHEEGGPPMEWTEEALALLQDKLKQSPPIAMEF
                     VTDMLKRDTEDLARVKKLTRIDASVLQDLWEAPREQIAWTDEAWKRLQTSPDFVRSGI
                     RKAAERRARKMGLKEIGSDQLTTFRNQAMMKAVKRIRSFGYHELTFDAFDTALQKTKR
                     LQGNDQAEKRLQEIRGHFADPMTKKPEGGTLGAELMDRFRRYLKGEGTL"
BASE COUNT         1324 a         1468 c         1377 g         1294 t
ORIGIN      
        1 gtatccctga tcctgcgatt cctccaggcc gtatcatcgg aatatcctgg ctgatacttg
       61 cccctctgct accggatcta tttgggggct ggcctgtcac atggcggaga gggaaaggag
      121 cacctcatgg atcgaagcat gatcgtaaca gcgtgggaac aacattgtgc cgacggttgg
      181 cctcagtttt cgagcccgga tcaagggcaa ttgatgacga tcgacacggt cattagcggc
      241 tgtgtggtct attttctcga tagttccgaa ggcctcgacg atcaacgggt tgccatcgtg
      301 aaagactgtt tgggagatct cgatgaactg acggaagcat tggacaccgg gtcccaagcc
      361 tacttttacc ggcttcgtaa actcggggcg atgttgctgg gcgatgagcc acagtcatga
      421 tgcgaggata ccgtgtccac tcccgttcct tgcacgtcta gcggctcttg gcatacaatc
      481 tgtcacatgt tcatctcgat tctatcgatt cacccatcct gatcaagtcc tgatcaagtc
      541 acgaacccat acgaaagaaa cgctaaatac catgacgaac gcgcgtgtga tacggatggc
      601 aattattcta ctggcgagca tcctagcaac ggtcggctca gctcatgccg agccgtatga
      661 attaagcaag aacgatgtca tggaaccgaa ggccatttcg agcgctgaga tttcgctgtt
      721 cggcgtcaaa ctcggcgact cggaatccaa agccgtcgaa acgctcgtga acgaaaagat
      781 ttccggaatc aaggctgagc aggaagcgac cttcatcttc ctgcttgatc aacgaaaacc
      841 gatcggtcca atggccgggg tgcgtattca ggacggtaag gtcgacctcc tttttattaa
      901 taaccggttc gcatataaga ccagaggtat ctttcgtaac gtccttaaca gcgaaagccc
      961 agaggacatc cgaaagttgt taggccagga ggagtatggt gacgaaaacg tcatgggcgc
     1021 cgtcatggct tacgacaagc aaggttttca ggtgaattac cttggcaaag acgtcaacat
     1081 tgagtttgcc gccccacggt agtctcgcca cgacgacatg acatcatcac atcatcaatg
     1141 gacagatcta accttcagac aaacgaaata gaaccactgt cccaggccga cataccatac
     1201 cggtacgtac actaccgctt ggctggtcta tgagtaaaaa tgccacggat gagtggcaga
     1261 gctgctattg gacggtagtc gtgagacgga atccgtaatc gatcaggctt ttcgcagtat
     1321 tccatcggcg gttagagttg aggatcacca ggagcagatc gctaccattg tgagagactt
     1381 tagcaatcag gcatcgacct gctttggagg tgaatccggt cttcacccct tccacgccgg
     1441 ggatgcgacc cagtaaacgg ttcgtgttat gcaggacata ggcgcgatat ccactgacag
     1501 gtgtgataat ctcgcgttcc cctttgacga gttctcggaa caccgcgttt cgcatcgcca
     1561 cttcactgag tgtcgcgaga ttttcagctg tcgaatagtg gtcgggaccg tcaaaaccac
     1621 atccgttgct gaagtgcgtg tccgataatc caagggctag cgccttggcg ttcatcaacg
     1681 tcacaaactg ctcttcatcg cctccgacat gctcgacagc cgccaggcag gcgtcattgg
     1741 ccgacaccat catcatcgcc ttgagcaaat cgccgagtcg aaacacttct ccgactttca
     1801 gtcgtaggtg agtcttggga gctcttgccg cattcctgct cactgttgcc agatcatcca
     1861 ggcggccttt ctcaagaatg atcaacgccg acataatctt ggttagactg gccggtgaca
     1921 gacgtttctc aacttcgtgt tcaaataaga tacggcctga tcgcaattcc ttcaggagaa
     1981 tactgtgggc cggaattcgc ttccacggga gtggctgtgg aacataactg atggtgtgaa
     2041 cggcgcggtc cttgaagtta gcctccattg cagaggcagg aagaatcgac aaaccgatca
     2101 ttgagagaaa taacgatcca aagagaccaa agcgacagag ggttcgtccc aaattctgca
     2161 aacgaaaatc aggccggatg tggtccttct gtgccaaaaa cgaccgcctc tctcagaaaa
     2221 tgaacgattc ccgaacttta tcacctttcc gcccactatc aagaacttta tgaaaaaacc
     2281 ggagaaggtc ggccaaatca atccagaatc cgcaattgag gcagcgggcg tatatcctcc
     2341 tgttaatgag ggtatagtgg cgttccacca tcatgaatcc tctgcacttg agacactgca
     2401 tacatgtccc gctctctaac tatcgaagac gattcataat taacgcgaca catccggcca
     2461 gtaatccccc gccgaagagc gtcgtaccaa aatacacgaa cacgttggta cctccagccg
     2521 gttgagtccc ttcccacaaa tctcgaatcc caccctgcac catcagaaca gagagggtca
     2581 tcaaggcccc aatcatgaac caccatgcaa aggatcgaag cgccatcgta gtgttcgaaa
     2641 atttagagct gttttatcca gcggtcactc tagcgaaggc cctccaggct gtcaagaata
     2701 gaccgtgatc gctcgccgcc tactgtcttg tctccgtctt cgcaactggt ttgagcccat
     2761 ggtgaagaaa gatcgagaca ctaccacttc cgttgtcggc aatagccagt cccggttctt
     2821 ctaggccgga agtcgtgaca cgaaaagagg acagggcaaa cggaccggac ttcgtacgat
     2881 aatttcttgg agggtaatga aaggtgccat cacctttccc gaacaagatc gacaaatcat
     2941 ttgactgcag attcacgatc gcgacatctt ctaaccgatc gccattgaaa tcccgcgcca
     3001 acccaaagtt gggaccggca tcagcaccgg agtgcttgcc gggacggaat gtcgcgttgc
     3061 cgttgccgag aaacgtcgtg aagctatctt gctctccatt gatgacgagt agatcccggt
     3121 ggtgatcgtt attgaagtcg gcaaaactca cgcccagagg tcggtggcct gtcgaatagt
     3181 ctttgggatc atgaaaggac ccgtcaccgt tcccgagcca aatagagacg gcattcgaca
     3241 tgggtcctcc attcgtcacc accaaatcca atttcccatc agcgttgagg tcagacagag
     3301 cgatggatgt gggagtgtcg ccatgttcgt actgagcccc atgttggaat tcgccggtcc
     3361 cgttaccaag aaagacttta accttgtcgt tccgtagggc gacgacaaga tccgtatgat
     3421 ggtcgccgtt gatatcatcg gcggccaatg caatcggggc acgatgcacc ggataacgtg
     3481 gtccttcttc aaaccttcca tccccatgtc cgagcaagac catgacttca tctccaccgg
     3541 agcatgccaa ggcgacatcg gcgtgtcggt cttgattgaa gttgcccatc acgagggaac
     3601 gcggctcctg acagacatgt agctgaacct gatcactgaa tgtcccgtca ccattcccca
     3661 acagaatcga gagggtatta ctcgcaatat tggtggtgac gaggtcggtg aatgagtcat
     3721 gattaaggtc ttcggtgata acagtagtag gattcttgcc gaccttgtag ctggcaaaat
     3781 aataaaaagg gtcaggtggg ttgtagggct cgctcttgga acaggcccac aaaccgtgac
     3841 tgacgagaag agccagccat aacagggaga agagcgccct ggctgcatgc gatgtccgct
     3901 caatatgaat gttcacacag tctccgacac tccaaccagc tgagccaatg agtctatgag
     3961 ggtggaatga gaaggtccag tatacagagg cggcgtttcc cctcgcaatc tgcggcgggg
     4021 cttcatcagc ctttgaattt cgccttccgc ttgcgttatg atgcgatctc gcttttagga
     4081 ggaaggtcat gagcaaaatt gtgaacgtcg atattacgat gtacgggata gctgaagttc
     4141 tgatgtggtg tcttgatcgg aacaaaggac gtgtcccagg cgtagatacg ccgggcttca
     4201 aaaagatgca ggagttgttg gctcagaagc cccagtctgc ggactatttt acgcttgatc
     4261 agttctggaa gaaaaaagtg acgttgccct tgaccgaaga cgaggttgcg acgattgatc
     4321 gttgtctcta cgacattccc aatttcgaca acgaaccgct cccacaaatc cggcacaaat
     4381 tctggcctca gcaggtggaa acccactaac agaacactcc gaccaaattg gtgtagctgt
     4441 tgccgcaagc catgttgaac gtgaggggag gaagcgttcg gttatcagag tgttccttcg
     4501 cctttgagat accggcggaa acgatccatg agctcggctc ctagggtccc gccttcaggt
     4561 ttttttgtca ttggatcggc aaagtgtcct cgtatctctt ggaggcgttt ttccgcctga
     4621 tcattaccct gcaggcgttt ggttttctgg agagcggtat caaaggcatc gaatgtcaat
     4681 tcatggtaac cgaatgagcg gatacgtttg acggctttca tcatcgcctg attccgaaac
     4741 gtcgtcaact ggtcggaacc gatttctttc aatcccatct tgcgggctcg ccgttcagct
     4801 gcttttctga ttccactgcg cacgaaatcc ggagacgtct gaagccgctt ccatgcttcg
     4861 tcggtccagg caatttgttc ccggggtgct tcccataggt cctgcagaac cgatgcatcg
     4921 atacgggtca gctttttgac cctggcgaga tcttctgtat ctcgtttcaa catatcagtc
     4981 acgaattcca tggcgatggg cggcgattgc tttaacttgt cttgaagcaa cgccaatgct
     5041 tcttctgtcc actccatcgg aggcccacct tcctcgtgga ggtcaccaag cgcttccact
     5101 ttttcaaata attcgacatt cacttcgaga tgcccccgtt cctttgcgat ctcctcggca
     5161 atctgcttgg tcatcggtcg cataaattcg ggcaccgtat tcaatcgttc tttggccgca
     5221 gcggtccatg gaaggcgtcc gtcggccatc tcctcaacag cagaggccat gccggcttca
     5281 ccgcccatgc gtcccatcat ctgcccctta aactgctcga gaatctcctc ggtgatttcc
     5341 tcatacccca attcacgagc tttcttttca gccaaacgac ggaccatacc acgcagaaat
     5401 atcggtgcgc gctccattcg tttgagcgca ccgtcggtcc agtgaatttc cgttgtattc
     5461 gcc
//
LOCUS       DATNDD010000378         4841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2179658, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000378 DATNDD010000000
VERSION     DATNDD010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179658"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VN647_09130"
     CDS             complement(<1..585)
                     /locus_tag="VN647_09130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWG97232.1"
                     /translation="MKTLTPVIGVLIATVGCSSASPQHNLFAWEQSRIVDLTHSFGAN
                     TIVWPTEQDFKLVVQYAEDTPGGYYYASNRMEMPEHGGTHIDAPIHFAKGKQTLDQIP
                     IERLSGAGVKIDIAEQCARDRDYQIATWDIEQWETKHGRIPDHAIVLLSTGYARFWPG
                     RKDYLGTDLRGQEGVRELHFPGLHPEAAAWLVRER"
     gene            complement(879..1202)
                     /locus_tag="VN647_09135"
     CDS             complement(879..1202)
                     /locus_tag="VN647_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HWG97233.1"
                     /translation="MAEPIEEEIQKEVKAHKILIYGKGTKTMPMCGFTRETMQFFDKY
                     GYPYELIDVLSQPAKREALTKMTNWPTLPKVFIDGTFYGDTDILDPMAAKGEIEPLLK
                     KAFGK"
     gene            complement(1212..1451)
                     /locus_tag="VN647_09140"
     CDS             complement(1212..1451)
                     /locus_tag="VN647_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWG97234.1"
                     /translation="MITPDVLTDYIRKSLPDAVVTVTDRTGTMDHLKVVIVSDGFHGK
                     NLLDRHRMIYQALNMPMKDGRIHALELTARTRDEA"
     gene            1696..2130
                     /locus_tag="VN647_09145"
     CDS             1696..2130
                     /locus_tag="VN647_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97235.1"
                     /translation="MSEFKSGFFVGGESTNGRVLIVDDEPDIRKVVRMTLQKAGYDVL
                     EAENGEKAIEVINSGENRLLLDVIICDIRMPKVNGIEAIAYFRQNYPRVPLIVLTGFP
                     DTDMATSLLRQGVVDYLVKPVEGEKLKASVARAMEQRELAHL"
     gene            2127..3272
                     /locus_tag="VN647_09150"
     CDS             2127..3272
                     /locus_tag="VN647_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWG97236.1"
                     /translation="MMDWTTSPGKGVAATSSPNAHLRLQNERSESVPASSPIGVAIVG
                     AGRGGTALLDVLHQIGTIEIVGITDRDPAASGLQRARELNVPIYERVADLIKNHRISL
                     VMDVTGDPAMEPMLREQLSAEADIVSGQASRLLWTLVQHESILQTELLHAEKLAGIGS
                     FAAGIAHDMNNPLQLILGLAENLTDETDLDAIHLQARDIIDAVKRTTAICRDLTSYSR
                     RTSLRQDCLVNINGKLDEALKIARYAVALQDIDIRKLYQPDVVVKGNPDELLHVFVNL
                     ITNAVHAMEHHGTLTLETSAVAGTIQIRVSDTGCGIAPEVQSRIFEPFFTTKPPGKGT
                     GLGLYNIKNVIHRMNGSIGVESHIGRGSTFTLAFHEEPGADERSTRS"
     gene            3269..>4841
                     /locus_tag="VN647_09155"
     CDS             3269..>4841
                     /locus_tag="VN647_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWG97237.1"
                     /translation="MMETSTSLPSGTRWGLKAKIIVSILLVGLIPLIVGLGMAFWQGS
                     QEIREVSGESFEALATEAARKLDLLLAEEIAHTARIANDPMLIRELEHRRDARAVAKS
                     FPTVPSDLERRWAARDRAAIKSVMENPFSALLHEYFTGGYSAPGQLLPRVVRSSTKML
                     FLTDAQGMLVATMTEHPPFRHNETRWWQGAFNKAVGRLYIEDIRFDDQVNAYVFTISL
                     PVMDSLRYEVVGILHRVIDAKEFFSPSTHVTRFGKTGHVMLIDTNGIVVSCPILPTGV
                     PLSDPNLIPLVTPQHPGWVQASSDGHGGQDSSVIGFAPLPETGRAANDSISNGSWHTF
                     VWQSSDELFAPIQHLFTWVSVFGAVAVVLLASLGYVAAGRIVTPVRQLQQAAQAIGRG
                     ELRTPIQINTGDELEDLAEEFNRMNSQLKAAFAGLTDQVKLKTQEVQVLQQSTDQILD
                     AVPTPILLIDAHEVVRYINQAARESFKAHEPMTGTSLFTIIPLDPVVQKRLQAEFHIS
                     GDSALATDTISLETVP"
BASE COUNT         1095 a         1394 c         1258 g         1094 t
ORIGIN      
        1 ccgttcacgc accagccagg ctgccgcttc cggatgcagc ccaggaaaat gcagttcccg
       61 tacaccttcc tgtcctctca agtctgttcc caaatagtcc ttccgacccg gccagaatct
      121 ggcataaccc gtactcaaca ggacgatggc atggtcagga attctgccat gcttagtttc
      181 ccattgctca atatcccagg tagctatttg gtaatcccga tcgcgagcgc attgctcagc
      241 gatatcgatt ttgacaccgg ctccgcttag acgctcgatt ggaatctggt ctaacgtctg
      301 cttccctttt gcaaaatgaa tcggcgcatc aatatgcgta ccgccatgct cgggcatctc
      361 catacgattg gacgcatagt agtacccacc aggcgtgtcc tctgcatact ggacgaccag
      421 cttaaaatcc tgctccgtgg gccaaacaat cgtgttcgct ccgaatgaat gagtcagatc
      481 aacgatccgc gattgctccc aggcaaaaag attgtgctga ggactagcag aggagcatcc
      541 cacggtcgcg atcagtaccc ctatcacagg agtcagcgtc ttcataagcg ataaccccgt
      601 tctaaaaact tctgatcttg aagactgcaa cgcagtagca tcaccgctct ttcttcctac
      661 ctccttagcc tcggatcgaa cggatgcgcg gtgaggttcg cgccatagca gggattgctc
      721 aatcggcagg caagagatat tgggtgcgac ggcattgcga cgggagtcgc agcattagag
      781 tcgcacccaa gagaaaagaa cgaagttcgt cagccgtctg gattcaacac attatggcgc
      841 aaccgagtcc gcgcatccgt tcgtcgccga gacctctctt acttcccaaa tgcttttttc
      901 agcagcggtt cgatctcgcc cttggcggcc attgggtcga ggatatcggt atctccatag
      961 aaagtgccgt cgataaacac cttgggaagc gtcggccaat tggtcatctt cgtcagtgct
     1021 tcccgcttag ccggctgcga gagtacatcg atgagttcgt atggataccc gtatttgtcg
     1081 aaaaattgca tggtttccct ggtgaacccg cacatcggca tggtcttcgt tcccttgccg
     1141 taaatcaaaa ttttatgggc cttcacttcc ttctggattt cttcttcgat cggttcggcc
     1201 atggtctcct cctatgcctc atctctggta cgcgctgtta attcgagcgc atggatacgt
     1261 ccgtccttca tcggcatgtt taacgcttga tagatcattc gatgccggtc caagaggttt
     1321 tttccgtgaa aaccatctga gacgatcacg accttgaggt ggtccatcgt tccagtacgg
     1381 tcggtcaccg tcaccacggc atccggcaga gacttgcgaa tatagtcggt cagcacatcg
     1441 ggtgtaatca ccggcctctt ctcccctagc tttttcaata ccctagccga aattggcttg
     1501 aaaacgcaat gcgctcctcg ctggtggttt tcagcattgt ggtggtgcaa ctgagccact
     1561 aatgggaaag caggctggct tagcacataa gcagttgaaa gtatttcaat accactccat
     1621 cacaatttgg catcgacttt gcgcatatca ttgagcaggc gcacatattt tcccctcttc
     1681 accaaggagg tcatcatgag tgaattcaag tccggatttt ttgtgggagg agaaagcacc
     1741 aatggtcgag tgctgatcgt cgatgacgag cccgacatcc gcaaggtcgt ccggatgacg
     1801 ttgcaaaaag ccggctacga tgtcctggag gcggagaatg gagagaaagc cattgaagtc
     1861 atcaattctg gtgaaaatcg gctgctcctt gatgtcatca tctgtgacat ccggatgccg
     1921 aaagtgaacg gcatcgaagc gattgcgtat tttcgccaaa actatccgcg cgtgccgctg
     1981 atcgtcctga ccggattccc cgacacagac atggccacgt cattactccg acaaggtgta
     2041 gtggactatc tcgtgaagcc ggtggaaggg gagaaactga aagcttctgt cgctcgcgcc
     2101 atggagcaac gtgaattggc ccatctatga tggactggac gacatcaccg gggaagggcg
     2161 tcgcggcgac ctcttcaccc aatgctcatc tacggctcca gaacgagcgg agcgaatcgg
     2221 tgcccgcctc gtctccgatc ggagtggcta ttgtcggagc gggccgtggt ggaaccgcgc
     2281 tactcgatgt gctccaccaa atcggcacga ttgagatcgt cggcattacc gacagggatc
     2341 ccgcggcatc aggacttcaa cgggcccggg agctcaacgt gccgatctat gaacgagtcg
     2401 ccgatctgat caagaaccac aggatcagtc ttgtgatgga tgtcaccggt gatcccgcga
     2461 tggaaccgat gctccgggaa cagctgtcgg cagaagccga catcgtcagc ggacaggctt
     2521 cacgacttct ctggacactc gtgcagcatg aatcgatatt gcaaaccgaa ttgctccatg
     2581 cggagaaact cgcaggaatc ggctcgtttg cggcgggcat cgcccacgat atgaataatc
     2641 cgcttcagct gattctcggc ctggcggaaa atctgacgga tgaaacggac ttggatgcca
     2701 tacatcttca agcccgggac attatcgacg cggtcaagcg cacgaccgcc atttgtcgag
     2761 atctcacttc ttactcacgc cgcacatcct tacggcaaga ctgcctggtc aacatcaacg
     2821 gcaagctgga tgaagccctc aagatcgctc gctatgccgt ggcgcttcaa gacatcgaca
     2881 ttcgcaagct ctatcagcct gacgtcgtcg tgaagggaaa ccccgatgaa ctccttcatg
     2941 tgttcgtcaa tctcatcacg aatgccgtcc atgccatgga acaccacggc accttgacgc
     3001 tggaaacctc agcggtggcc ggcacaatac agattcgtgt ttctgacacc ggctgcggca
     3061 ttgccccgga agtgcaaagt cggatcttcg aacccttttt caccacgaag ccaccgggga
     3121 aaggaacggg attgggccta tacaacatta aaaatgtcat ccatcgcatg aatggctcta
     3181 tcggggtcga gagccacatc ggccgcggct ctaccttcac cctcgctttt cacgaagagc
     3241 ccggggccga tgagaggagc actcgatcat gatggagacg tcgacatcat tgccgtctgg
     3301 caccaggtgg ggcctcaagg caaagatcat cgtctccatt cttcttgtcg gactcatccc
     3361 gctgatcgtc ggcttgggaa tggcattctg gcaggggtct caagaaatcc gggaggtcag
     3421 cggagaaagc ttcgaagcac tggcgactga agccgcccgc aaactcgatc tcttgctcgc
     3481 cgaagaaatc gcccacacgg cgcgtatcgc gaatgatcca atgctcatcc gagaactgga
     3541 acatcggcgt gatgcacggg ctgtcgccaa gagttttccc acggtcccct cggacttaga
     3601 acggcgttgg gcggcacgag accgagcggc gatcaaatcc gtcatggaga atccgttcag
     3661 cgccttgctc cacgaatact ttaccggcgg ctatagcgcg ccgggacaat tgcttccgcg
     3721 cgtagttcgc tcctcgacga agatgctgtt cttgaccgat gcacagggca tgctcgtcgc
     3781 gaccatgacc gaacatcccc cttttcgcca caacgagacc cgatggtggc aaggggcctt
     3841 caacaaagcg gtgggcagac tctacatcga agatatccgt ttcgacgacc aggtgaatgc
     3901 ctatgtgttc accatttcac ttccggtcat ggacagcctc cgatatgaag tcgtcgggat
     3961 acttcaccgc gtgatcgacg ccaaagagtt tttttctcct tcgacccatg tcactcgatt
     4021 cggaaagacc ggccatgtca tgttgatcga taccaacggc atcgtcgtca gttgcccaat
     4081 tcttccgacc ggcgtcccgt tatccgatcc gaacttaatc ccgcttgtca caccgcaaca
     4141 tcccggttgg gtgcaagctt cgagcgacgg tcacggagga caggatagct cggtgatcgg
     4201 tttcgcccct cttccggaaa ccggccgagc ggccaacgac tctatcagca atgggtcgtg
     4261 gcacacgttc gtctggcaat cgtccgatga actcttcgcg ccgattcagc atctgttcac
     4321 ctgggtgtcg gtcttcggag ccgtcgccgt tgtactgttg gcttctctgg gttacgttgc
     4381 cgccggccgc atcgtgacac ccgtgcgaca actgcaacag gctgcgcagg ccattggacg
     4441 cggtgaactg cggacaccca ttcagatcaa caccggggac gaattggaag acttggcgga
     4501 agagtttaat cggatgaaca gtcaactgaa ggccgcattc gccggactga ccgaccaggt
     4561 caaattgaaa acccaggagg tccaggtcct ccagcagtcc acggatcaga tcttggacgc
     4621 cgttcccact cccattctct taatcgatgc tcatgaagtc gtgcgttata tcaatcaggc
     4681 ggcccgcgaa tccttcaagg cccatgaacc gatgacggga acgtcactgt tcacgattat
     4741 tccgctcgat ccagtcgttc agaaacggct tcaagcggaa tttcacatca gtggggattc
     4801 agctctcgcc actgacacga tctcccttga gactgtgccg a
//
LOCUS       DATNDD010000379        10964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1872233, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000379 DATNDD010000000
VERSION     DATNDD010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872233"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VN647_09160"
     CDS             <1..555
                     /locus_tag="VN647_09160"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HWG97238.1"
                     /translation="RISPYYKQLISSGQGGTPETKAYMDEKLRAAQWVIRSIEQRNRT
                     IVKVVSSIVKFQEQFFDHGVQYLKPLVLKQVAEDIGMHESTISRVTANKYMYCPQGML
                     ELKFFFNAGLQRADEPSGMHSSVSVRDMIKTMVAEEDAKRPLKDEEIAARLRKQGVLI
                     ARRTVAKYRAELNISSASQRKRFF"
     gene            600..938
                     /gene="raiA"
                     /locus_tag="VN647_09165"
     CDS             600..938
                     /gene="raiA"
                     /locus_tag="VN647_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWG97239.1"
                     /translation="MKLRITGRHMDITPALRSYVETRFGRLDRYGLKIGSLQVVLGIE
                     KLQHKAEVIGAVSGKRMQAKTSTPEMYATIDALVDRVDAQFRKRKDRLVNHKPTKPKR
                     SRSSSHVSLS"
     gene            1052..1918
                     /gene="rapZ"
                     /locus_tag="VN647_09170"
     CDS             1052..1918
                     /gene="rapZ"
                     /locus_tag="VN647_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWG97240.1"
                     /translation="MARLNLVIISGLSGSGKTYALKAFEDAGYFCIDNLPLALLPTFV
                     DLCNQQHSEIANVALGIDIRERAFFSDFVGILERVKALGHAVQLLFLEAREEVLIRRF
                     SESRRPHPLLPHLPVLDGISFEKERVAELRRRADRIIDTSDLTVHELGELLAKQFLRA
                     ASDRRLTISLMTFGYKFGVPYDIDLLFDVRFLKNPFFVPELKPLSGDDPRIGTFVLTD
                     PEAISLMEHLEGLLKFLLPLFQRERRSYLTIGIGCTGGRHRSVAVANRLHESLSALGY
                     EVGLKHRDIDKT"
     gene            2043..2255
                     /locus_tag="VN647_09175"
     CDS             2043..2255
                     /locus_tag="VN647_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWG97241.1"
                     /translation="MKHPKYALSEAGFKAYQVCNLFDISKATLFRWEREGVITQPARD
                     WRNWRLYTRKNVDEIEKVIRARKVVA"
     gene            2271..3377
                     /gene="pilM"
                     /locus_tag="VN647_09180"
     CDS             2271..3377
                     /gene="pilM"
                     /locus_tag="VN647_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HWG97242.1"
                     /translation="MLISLKKLVETDIIAMLTPRRQLVGLDIGSSAIKVAQLKESKGR
                     YFLQKFGVKPLEPEVIVDGTVMDEGRVISAIGELFEEANVKNKHVAISISGHAVIVKK
                     INLPPMPDEELEGQVKLAAEQYIPFDINEVNIDFHVLPSDASEDQQGDMSVILVAAKK
                     DKINELTELVKAAGLMPMVMDVDAFAVENMHAINYPMAQEETTALVNLGASVMNVNII
                     RAGSSLFTRDIPLGGNRYTEAIQREIGLSFEEAEESKKNDRGGDSGGVSLTGVMDSVN
                     AEVASEIARTVDYFKTSTANAELSRVLICGGVARAKGLIQQLGDRMQLPVEMADPFAE
                     IDITGCDIAPDLLVDLAPSAAVGVGLALRAVGDR"
     gene            3374..3928
                     /locus_tag="VN647_09185"
     CDS             3374..3928
                     /locus_tag="VN647_09185"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWG97243.1"
                     /translation="MIRINLLPGGPKGRAAKPQYDVRAQALLGIGVILITLVGCWWYS
                     ASLDSELEARQEEKRDKEKQVAQLKEQVKKVQDFELRKKLLEDKNRIIDQLEQSRMGP
                     VKVLDHVSQSLEPLKVWLTKLGVTSDMVELEGKALTNDDVVEFVNNLRRTDYFTGINL
                     QESKAAIENKINLYQFRLAFRLKG"
     gene            3937..4575
                     /gene="pilO"
                     /locus_tag="VN647_09190"
     CDS             3937..4575
                     /gene="pilO"
                     /locus_tag="VN647_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HWG97244.1"
                     /translation="MPQLNLDALRNVPASQKAALLFLLVGGMIAGFYFYIAEPKSATI
                     ASLEAENGKLESEIQTLTIKVKHLDELVAANKQLEIELAKKKERLPPEEEAIMLLKQV
                     SDLGVRLGLDIRLWKPGAQTEDASKLFVKMPVSVEVAGVYHTAALFFDRINRLPRIIT
                     VSGLKMGSPKIEQGRIVSQTTFDLVAYAAPQDKPVSGASPSGNAPKVAQVGK"
     gene            4591..5214
                     /locus_tag="VN647_09195"
     CDS             4591..5214
                     /locus_tag="VN647_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HWG97245.1"
                     /translation="MYLWDIRKKTIEYSLTVSVGVVCACVILSGNIDAQAESSPVPRP
                     MIPMRQDALKLSSVQEIPKTAPQTLEASLSGSNTPVPSQLSIPDGMVGGGYDPSGRRD
                     PFAPIVQELQPGKMDTTLPPLQRVTLTELNLIAIVWGTYGYTAMVQTPEGHGYTVRRG
                     TRIGQNNGVVSAITERGIIVQERFTDVYGKKQEREHVKLLHPKEGSE"
     gene            5211..7178
                     /gene="pilQ"
                     /locus_tag="VN647_09200"
     CDS             5211..7178
                     /gene="pilQ"
                     /locus_tag="VN647_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HWG97246.1"
                     /translation="MKPLFLRADNSLTAGCFIGVLGLSLAALAIFAETPAFAEEIGMT
                     KRGEATTSRLLAQAVTAIEVRPETDLVTVVVAGDGQLFPEANFLDESRLIVDIPAVSS
                     ALRRSVIRADHYLLKKIRVGHHADKVRLVFDVSERPIFSLAREENKVLITLKPSEHKI
                     PTVVAAQFDGERERILFTPRPRKMLFESGDRVGKRMMRMVGPTQSQFKVQRVQMAGDS
                     VPVEKADRSDDIVAGQTRYVGRRISLDFQQADITNILRLIAEVSGFNIVVGEGVKSKV
                     TMKLVSVPWDQALDMLLKMNGLGMIRQGSIVWVDSLANIAKQQDEEARAKDSKTKAEE
                     LIDRVFYIRNLQAQEVMTSLRQYLSPRGVMQFNQGSNALIVRETESKLAIVKQLVEGL
                     DLEVPQVQIEARIVQADTVYARGLGIQWGFQAGNRTPTDFFTLSNITGPFGQIAGTGN
                     TTIDRTFLVNLPAQVGGLPAVPSIGWTFGKLGGDFALDMRLSAGELLGLSKVIAAPKI
                     TTLDKREAKISQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEEGKRILMR
                     VRATRNAVGARSNPAGPSIDRREATTQVIVKDGETMVIGGVFVDTQSNNVQGVPYLSR
                     MPVLGWLFKNKSETVSKQELLIFLTPSIIKV"
     gene            7426..8535
                     /gene="aroB"
                     /locus_tag="VN647_09205"
     CDS             7426..8535
                     /gene="aroB"
                     /locus_tag="VN647_09205"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWG97247.1"
                     /translation="MTPTSTVKVSLPERSYEIIIRAGLLERLGRELKRYGVKGKVGVV
                     TDRHVARHYLKQISESVRRYEIEPVPIILAPGERSKTLGTVERILDMLARHRFERSSM
                     LLALGGGVVGDMAGFAASIYQRGIPYIQVPTTLVAQVDSSVGGKTGVDHRLGKNLIGS
                     FYQPCAVWVDPLTLHTLPPRELVAGLAEVIKYGIIADEPFFVYLQRHMSELRRQAPGI
                     VATVVKRSCEIKAEVVAADERESDRRRILNYGHTIGHALEALGGYKAFVHGEAVGIGL
                     VQEAELACFQGICARTVVEEIRRTVNEAGLSDRMPRWAPQRIWKAMLHDKKVSEGRVI
                     GVWPERIGQVRIGPIEKHVFEQWYSVARVSPHGHA"
     gene            8634..10418
                     /locus_tag="VN647_09210"
     CDS             8634..10418
                     /locus_tag="VN647_09210"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWG97248.1"
                     /translation="MRTLRIAMAQMNPTVGDLIGNIRRITTWLRDAKEAKADLVVFPE
                     LAITGYPPEDLLLKPQFVADNLRALNEITQACRGLVAVVGYVGQEDQSNSHSSRRSIV
                     SAGRHTLYNSAALIADRKLVGSYSKWYLPNYGVFDESRYFRPGRRLPLLLVNGTTIGV
                     TICEDIWLQEGPTRAQAAAGAEVIVNINASPFHVGKSRLREQMLATRARENGVIVTYT
                     NMVGGQDELIFDGNSLILDQSGNVIARGHAFREELLVADLNVDAASPGRRPYGRKAAL
                     TGKAVSAVDRLVVNETAINKKKRTRIVPGVAKPLDEIEEVYRALVLAVKDYVRKNGFT
                     RAVIGLSGGVDSAVTAAIAADALGAKHVLGVFMPSPYTSHESGEDVTELARCLGIELS
                     TIPITPTFEAYRQSLAPSFVDRSEDATEENLQARIRGNLLMALSNKFGHLVLTTGNKS
                     ELSVGYSTLYGDMAGGFAVIKDVPKTMVYGLTRFRNELGPSPVIPKRILDRPPTAELR
                     PNQKDEDSLPPYTVLDPILRAYVEEDRSLDEIVAAGFDRATVSRVVRLVDSSEFKRRQ
                     APIGVKITHRAFGKDRRMPITNGYRIKP"
     gene            10777..>10964
                     /locus_tag="VN647_09215"
     CDS             10777..>10964
                     /locus_tag="VN647_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWG97249.1"
                     /translation="MKLVEAIVKPFKLEEIKDALLEIGIQGMTVSEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEV"
BASE COUNT         2677 a         2589 c         3141 g         2557 t
ORIGIN      
        1 aggatcagcc cgtattacaa acagcttatt tcttccggac agggcggaac tccggaaact
       61 aaagcttata tggatgaaaa attgcgggcc gctcaatggg ttattcgaag catcgagcaa
      121 cgaaatcgga ctatcgtgaa agtggtttcc agcatcgtca agtttcaaga gcaatttttt
      181 gaccatggcg tccaatatct caagccctta gttctcaagc aagtggccga agatataggg
      241 atgcatgaat cgacgatcag tcgtgtgacg gccaataaat atatgtattg tccacagggc
      301 atgttggaac tcaagttctt cttcaatgcc gggcttcagc gagctgatga accctcaggc
      361 atgcattcct cggtttcagt cagggatatg atcaaaacaa tggtggctga agaggatgcg
      421 aagcgtccct tgaaagatga agaaattgcc gctcgacttc gtaagcaggg ggtgcttatc
      481 gcacgcagga ctgtggctaa atatcgggcc gagttgaaca tctcatccgc cagtcaacgt
      541 aaacgatttt tttgatcatc gttgcttcgt atccggccag gtctaaattg gaggtgggca
      601 tgaagctgag aatcacaggt cgccatatgg atatcacgcc agcgcttcga agctatgtgg
      661 aaacacgatt cggtcgcctg gatcgatacg ggttgaagat cggatcactt caagtggtat
      721 tgggaataga gaaacttcag cataaggctg aagtcattgg tgccgtcagt ggcaaacgaa
      781 tgcaagccaa gacatcgact cctgagatgt atgccacaat cgatgctctc gtagaccgtg
      841 tggatgcgca gtttcggaaa cggaaagatc gccttgtcaa tcataagccg accaagccga
      901 agagatcacg atcgagttct catgtgagtc tctcgtgaaa tctcgctccc tttcctcctt
      961 gacgcgctca atgtgccaga gagactgact ggcatgactc tcaataggag tgtctcggta
     1021 tgctcatcga ggcaagtacg agtacgacct catggcccgg ctgaatctgg tcattataag
     1081 cgggttgtcc ggatccggta aaacctatgc cctcaaagca tttgaagatg ccggatattt
     1141 ctgcatcgat aatcttcccc tcgccttact tccgactttt gttgatctct gcaatcaaca
     1201 gcatagcgaa attgccaatg ttgcccttgg gattgacatt agggagcgtg cattcttttc
     1261 ggatttcgtc gggatcttgg aaagagtgaa agctctgggc catgccgtac agttgctttt
     1321 tcttgaagct cgggaggagg tgctgatcag acgattttca gagtccagac gtcctcaccc
     1381 gcttttgccg catctcccgg tcctggatgg gattagtttc gaaaaggaac gtgtagccga
     1441 acttcgacgc cgagctgatc gaatcattga tacgtccgat ctgacagtcc atgagctcgg
     1501 tgagttactt gcgaaacaat tcctacgggc ggcctcggac agacgactca cgatctcact
     1561 catgacgttc ggatataaat ttggggtacc gtacgatatc gatttattgt ttgatgtgcg
     1621 atttcttaag aatccattct tcgtgcctga gctcaagccg ctttccggag acgatccacg
     1681 aatcgggacc ttcgttctga cagaccccga ggccatttca ctcatggagc acctcgaagg
     1741 cctgctgaag tttctcctgc cgctattcca gcgagagcgg cgtagttatt tgaccatcgg
     1801 tatcggatgt accggtggac gccatcgttc agtggcggtc gctaatcgtc tccatgaaag
     1861 cctctctgcg ttgggatacg aagtcggcct caaacaccga gatatcgaca aaacgtaagt
     1921 tgtttaaacc acagccatca ttttcctgcc gtgacttcgg tcgcttcctt gacaggtaga
     1981 gcatattgac tatacttagc cttgtgagta tccgaaggcg tcagagcagt gtaaatacta
     2041 cagtgaaaca tcctaaatat gcgctgagtg aagcgggctt caaggcctat caggtatgta
     2101 acttgttcga catttccaag gcgacgctct ttcgttggga acgggagggt gtcattaccc
     2161 aacccgctcg ggattggaga aattggcggc tctatacgag aaagaacgtg gatgaaatcg
     2221 agaaagttat tcgcgcccga aaggttgtcg cataaagtgg caggtaacgt atgttgatct
     2281 cactcaaaaa gctcgtggag accgatatca ttgcgatgct gactccccgc cggcagttag
     2341 tgggacttga tatcggatcg agcgcgatta aggttgctca gctcaaagaa agtaagggac
     2401 ggtactttct ccaaaagttc ggcgtcaaac cgcttgagcc ggaagtgatt gtggatggga
     2461 cggtcatgga tgaagggcgt gtgatttcgg ctattggaga attgtttgag gaagccaacg
     2521 tcaagaataa gcacgtcgcc atctcgattt cggggcacgc tgtcattgtg aagaaaatca
     2581 atctgccgcc catgccggac gaagagttgg aaggacaggt gaaattggca gcggagcagt
     2641 acatcccctt cgatatcaac gaagtgaaca tcgactttca tgtcttgcct tcggatgcgt
     2701 ctgaagatca gcagggggat atgtcggtga ttcttgttgc cgctaagaaa gacaagatta
     2761 atgaattgac tgagctcgtc aaggctgccg gactcatgcc aatggtcatg gatgtcgatg
     2821 cgttcgcggt tgagaatatg cacgcgatta attatccgat ggcgcaggaa gaaacgacgg
     2881 cgctggtgaa tctcggcgcg agcgtgatga atgtgaatat catccgtgcc gggtcgtcgt
     2941 tgtttacgcg tgatattccg ttggggggga ataggtacac cgaggcgatt caacgcgaga
     3001 tagggctctc ctttgaggaa gccgaagaga gtaagaaaaa cgatcgagga ggcgattccg
     3061 gcggggtgtc gcttaccggc gttatggaca gtgtgaatgc ggaagtggcg tccgagattg
     3121 ctcggacggt ggactacttc aagacctcga ccgcgaatgc tgagctcagt cgtgttctca
     3181 tctgtggtgg cgtagcccgg gcaaaagggc tgattcaaca gcttggcgac aggatgcaac
     3241 tgcctgtaga aatggctgat cccttcgccg aaatcgatat tacagggtgt gacatcgccc
     3301 ctgacctact ggtggatttg gctccgtcgg cagccgtcgg tgtcggattg gcgttgagag
     3361 cggtggggga cagatgattc ggatcaacct ccttcccggt gggcccaaag gaagagcggc
     3421 caaacctcag tatgacgtgc gcgcacaggc gctgctcggc atcggcgtga tcctgattac
     3481 actggtcgga tgctggtggt attcggcttc attggacagc gagcttgaag ctaggcagga
     3541 ggaaaagcga gataaagaaa aacaggttgc tcagctgaaa gaacaagtca agaaagtaca
     3601 ggattttgaa ctgcgcaaaa agcttcttga ggacaagaac cggattatag atcagctcga
     3661 gcaatccaga atgggtccgg tgaaggttct cgatcatgtt agtcagagtt tagagccgct
     3721 caaagtatgg ctcacaaaac taggtgtgac atccgatatg gttgagttgg aagggaaggc
     3781 cctaacaaac gacgatgtgg tggaatttgt gaataacttg cggcgtacgg attatttcac
     3841 cggtatcaat ctgcaggaga gtaaagctgc aatagaaaac aaaatcaatt tgtatcagtt
     3901 tcgtttggca tttcgtctga agggttaatg atggcaatgc cacagctcaa ccttgacgcg
     3961 cttcgcaatg tgccggcttc gcaaaaggcc gcgctcttat tcttgctcgt cggcggcatg
     4021 atagccggat tctatttcta tattgcggaa cctaaatccg caactatcgc gtcgcttgag
     4081 gctgaaaacg gtaagctgga aagtgaaatt caaacgctca cgatcaaagt gaaacatctt
     4141 gatgaattag ttgccgcgaa caaacagctc gaaattgaat tggctaaaaa gaaagaacgc
     4201 ctcccccctg aagaagaagc gatcatgctc ctgaagcagg tgtctgacct tggggtacga
     4261 ttgggactcg atatcagact gtggaagcca ggcgcgcaga ctgaggatgc gtcgaagctc
     4321 ttcgtcaaga tgccggtcag tgtggaagta gccggggtct atcacactgc cgcactgttt
     4381 tttgatcgga tcaatcgact ccctcggatt attacggtgt cgggccttaa gatgggatct
     4441 ccgaagatcg aacaaggacg aatcgtgtcg caaacgacat ttgatttggt ggcatacgcc
     4501 gcccctcagg acaagccggt ttcgggggca tctccttcag gcaatgctcc caaagtagct
     4561 caagtgggaa aatagcgaaa tcgtgacagc atgtacctct gggatatcag aaagaagacc
     4621 atagaatatt cgctgaccgt cagtgtcggc gtggtatgcg cgtgtgtgat cctttcgggg
     4681 aacatagatg cacaggccga gtcttccccc gttcctcgcc cgatgattcc gatgcgacaa
     4741 gatgccctca agctatcttc tgtacaggaa atcccgaaga cggctccgca aactttagaa
     4801 gcgtcactgt cgggctcaaa taccccggtg ccctcccagc tttccatacc ggacggcatg
     4861 gtggggggtg gctatgatcc ttccggccgt cgagacccat ttgctcctat tgtccaagag
     4921 cttcagccgg gaaagatgga tacaaccctt ccaccattac aacgcgtgac tctgaccgag
     4981 ctaaacctta ttgccattgt atgggggaca tacggctata ccgctatggt gcagactcca
     5041 gaaggacatg ggtacacagt tcgacgggga acgaggattg ggcaaaataa tggagtagtc
     5101 agcgcaatta ccgagcgcgg catcattgtt caggaacggt tcactgatgt gtatggcaag
     5161 aagcaggagc gagaacatgt caagcttcta catcctaaag agggctcaga atgaaaccac
     5221 tatttttacg cgcagataat tccctaactg ctggatgttt catcggagtc ttggggctgt
     5281 cgctcgccgc tctggccata tttgccgaga cacctgcatt cgctgaagag attggcatga
     5341 caaagcgagg tgaggccacc acctcacgat tgctggcgca ggccgtgacc gccattgagg
     5401 tccgtcccga aacggatctg gtgactgtgg tggtcgccgg ggatggtcag ctatttcctg
     5461 aggccaattt tttagatgaa tcccgcttaa tcgtcgatat tcctgccgta tcgtccgccc
     5521 ttagacgatc agtgatacgg gccgaccact acctgctgaa gaaaatcaga gtgggacacc
     5581 atgctgataa ggtcaggctg gtgttcgatg tgtcggaaag gccgatcttt tcccttgccc
     5641 gggaagaaaa taaggtattg atcacactga aacccagcga gcacaaaata cccactgtgg
     5701 tcgcggcgca gtttgatggg gaaagagagc gcattttatt cacccccagg ccacgcaaaa
     5761 tgttgttcga gtcgggggat cgtgtgggca aaaggatgat gcgaatggtc ggtcccacgc
     5821 agtctcagtt taaagtgcag cgcgttcaga tggcgggaga tagtgttccg gttgaaaagg
     5881 ccgacagatc cgatgacatt gtggcagggc aaacacgata tgtcggccgg cgtatctcgc
     5941 tggattttca gcaggccgac attaccaaca ttcttcgatt gatagccgaa gtcagcggct
     6001 tcaacatcgt cgtgggagaa ggcgtcaagt cgaaggtcac catgaaattg gtgagtgtac
     6061 cgtgggatca ggcgctggat atgcttttga agatgaacgg cctcggcatg attcgccaag
     6121 ggagcattgt atgggtggat tctctggcga atattgccaa gcagcaggat gaagaagcac
     6181 gggccaagga ctccaagacc aaggcggaag aattaatcga ccgcgtcttt tatatcagga
     6241 atctccaggc gcaagaggtt atgacatcac tgcggcaata tttaagccca cgcggtgtca
     6301 tgcaattcaa tcagggaagc aatgcgctca ttgtgcgcga gacggaatcc aagttggcaa
     6361 tcgtcaaaca gctcgtggag ggactcgact tggaagtccc tcaagttcag attgaagcgc
     6421 gcatcgttca agcggacacc gtgtatgcga gaggcttggg gattcagtgg gggttccaag
     6481 cgggcaatcg cacacccacc gatttcttta ccctctcaaa tattaccggt ccgtttggac
     6541 agatcgccgg aacagggaac accacgatcg atagaacctt cttggtcaac ttaccggctc
     6601 aggtcggtgg gttgccggct gttccgtcga ttggatggac ctttgggaag cttggcggcg
     6661 atttcgctct ggatatgcgg ctctcggccg gagagttgct ggggttgagc aaagtgattg
     6721 cagcgccgaa gatcaccacg ttggacaagc gggaagcgaa gatttcccaa ggcgaatcga
     6781 ttcccttcca gacgacatcc ttacagggta cgcaaacgac atttgtcgat gcgaacttgg
     6841 aattgaacgt aacgccgcaa atcacctcgc gtgatccgaa ggaagagggg aagagaattc
     6901 tgatgcgcgt ccgagcaact cggaatgccg tcggcgcacg gagcaatccg gctgggccga
     6961 gtatcgatcg acgagaagcc accacgcagg tgatcgtcaa agatggtgaa accatggtga
     7021 ttggtggtgt cttcgtcgat acgcaaagca acaacgtgca aggtgttccc tacctttcac
     7081 gcatgccggt tcttgggtgg ttgttcaaga acaaatccga gaccgtgtcg aagcaggaat
     7141 tgctcatttt cctcaccccc agcattatta aagtctagga cctgtttcat ctccgtgatc
     7201 agcaaaagcc tggtgacgcg cttcagcatc actgggcttt tgcttttttg tcttccggtg
     7261 atcgtgggaa taccgcccag agttgtggca ctcgtcccga aacgtaaccg tctttttagt
     7321 tcatcggggc tgtgttacga tgcttctccc ttcgatgttt catgtgtggc tcccgaatgg
     7381 acatgacacg aaattctcaa gttcggtcag cgattgggtt acgaaatgac tcccacctca
     7441 acggttaagg tttccctacc tgaaaggagc tatgagatta tcatccgagc cggactttta
     7501 gagaggctcg gaagggaatt gaagagatat ggagtaaaag ggaaggtcgg agttgtgacg
     7561 gatcgccatg tcgctcggca ttatctaaaa cagatttctg agtcggttag gcgatatgag
     7621 attgagccgg tgccgatcat tcttgcaccg ggggaacgat cgaaaacgtt agggacagtg
     7681 gaacgtatcc tcgacatgtt ggcgcgtcat cgatttgagc ggtcgtctat gctcttggcg
     7741 ctcggcggtg gtgtcgtggg cgatatggcc gggtttgctg cttcgatcta ccagcgtggt
     7801 attccctaca ttcaggtgcc gacgactctt gttgcacagg tcgattccag cgtcggagga
     7861 aagacgggtg tcgatcatcg gctcgggaag aacctgatcg gatcgtttta tcaaccatgc
     7921 gccgtatggg tagatccatt gacgcttcat accttgcccc ctcgggaatt ggtcgccggg
     7981 cttgccgagg tcattaagta cgggatcatt gcggatgagc cgttctttgt ctatctgcag
     8041 cggcacatgt ccgaactgag gaggcaggcg cctggcatcg ttgcgacagt ggtgaagcga
     8101 tcctgtgaga taaaggccga ggtggtcgcc gcagatgagc gagaatccga tcgcaggcga
     8161 atccttaact acggtcatac catcggacat gctcttgagg cattgggggg gtataaggcg
     8221 ttcgtccacg gagaagcggt ggggatcgga ttagtacagg aagccgaact ggcctgtttt
     8281 caaggaatct gtgctcgtac agtggtcgaa gagattcggc gcactgtgaa cgaggcggga
     8341 ctgagtgacc gtatgccacg gtgggcaccc cagaggatat ggaaggccat gctgcatgat
     8401 aagaaggtgt cagagggacg agtcattggg gtatggccgg aacgtatcgg acaggtgcgg
     8461 atagggccga ttgaaaaaca tgtgtttgag cagtggtatt cggttgcacg agtctctcct
     8521 catggtcacg cctaattacg cagctcctgc ccaagggacg aagtcgatat gaacctatct
     8581 tcggtgtaga gtgcactgga ccatcgggga taggacaggc ttaccggagc gctatgcgga
     8641 cgttgcgaat cgcgatggca cagatgaacc caactgtcgg agatctgatc ggcaacattc
     8701 gccgtatcac gacctggctt cgagatgcga aggaggccaa agccgacctg gttgtatttc
     8761 ctgaactggc cattaccggt tatcctcccg aagacctact tctcaagccg caatttgtgg
     8821 cagataactt acgggcactg aacgagatca cccaagcctg tcgcgggttg gttgccgtgg
     8881 tagggtacgt ggggcaggaa gatcaaagta actcacattc ctcccgtcgg tcgatcgtct
     8941 cagccggccg gcatacactc tacaattcag cagcgttgat tgccgatcgc aagttagtcg
     9001 gtagttatag caaatggtat ttgccgaact acggagtatt cgatgaaagc cgttattttc
     9061 gtcccggacg gagactcccg ttgctcctcg tcaacggcac gacaatcggc gtgaccatct
     9121 gcgaagatat ttggttgcag gaagggccta ctcgcgcaca agcggcggct ggggccgagg
     9181 tgatcgtcaa tatcaatgcc tctccctttc atgtcggcaa aagtcgtttg agggagcaga
     9241 tgctggccac gagagcgcgg gaaaacggag tcattgtcac gtatacgaac atggttggag
     9301 gccaggacga gctgatcttc gacgggaaca gtttgattct ggatcaatcc ggcaatgtga
     9361 tcgcacgtgg tcacgcgttt cgggaagagc tgcttgtagc tgatttgaac gtggatgcgg
     9421 cttcacctgg acgtaggcct tatgggcgaa aggcggcatt gaccggaaaa gctgtttcgg
     9481 ccgtcgatcg tctcgtggta aacgagacgg cgataaacaa aaagaagcgg actcgaatcg
     9541 tgccaggggt agcaaagccg ttggatgaga tcgaagaggt gtaccgcgcc ttggtactgg
     9601 ccgtgaaaga ttatgtccga aagaatggat ttacgcgagc ggtgattggg ttgagcggcg
     9661 gcgtcgattc ggcagtgacg gcggcaattg ccgcggatgc gcttggtgcc aagcatgtgc
     9721 tcggcgtctt catgccgtct ccatacacct cacatgaaag cggagaggat gttaccgaac
     9781 tcgcgcggtg tctcggcatc gagttgagca cgatcccgat tactccgaca tttgaagcct
     9841 atcgacagtc gctggcaccg tcatttgtcg accgttcgga ggacgcaacc gaggaaaacc
     9901 tccaggctcg tattcgaggc aatctgctca tggctctatc caataagttc gggcatctgg
     9961 ttttgaccac tgggaacaag agcgagctga gtgtgggata ttccacccta tacggcgata
    10021 tggccggtgg gttcgccgtc attaaggatg tgccgaagac gatggtctac ggcctgacga
    10081 ggtttcgtaa tgaactcggt ccatcgccgg tgattcccaa acgtattttg gatcgaccgc
    10141 cgaccgccga gttgcgaccg aatcaaaaag atgaagattc ccttccaccg tatactgtgc
    10201 tcgatcccat tcttcgggcg tatgtcgaag aagatcgctc gctcgacgaa atcgtcgcag
    10261 caggtttcga tcgcgcaacc gtctcccgcg tggtccggct ggtcgacagc agcgagttca
    10321 agcgacgtca ggcacctatt ggcgtcaaga tcactcaccg cgccttcggc aaggatcgtc
    10381 ggatgccgat tacgaatggc taccgaatca agccataacc tccactggtt ctgcattcca
    10441 agcatatcat tactgttcgg acatttatcc gggattccgg atgaaaatga attgccaagg
    10501 ctgcttcgcc cttggatcgc attgttgtaa ccgctcaatg cacagagcgt gggacataaa
    10561 gaggtcgagc aattttaaag gatttacagg atttccacgt tgagtgagag gaaaggatgg
    10621 gctatgatgg ctgccccaaa gctgagggaa agccggaggg gccatggctt ttccgtgagg
    10681 agaagcatgg ttcgtcaatt agctttgcac ggctccttca tgggcatctg agagccgtcg
    10741 gggattcctg agctggtagg gcaaggaggc atcgatatga agctggttga ggccatcgtc
    10801 aaaccgttca aactggaaga gatcaaagat gccctattgg aaatcggcat ccaggggatg
    10861 actgtatcgg aagtcaaagg gtttgggcgt cagaaaggcc ataaggaaac ctatcgaggc
    10921 caagagtata caattgaatt tgtccccaag gtgaaaatcg aggt
//
LOCUS       DATNDD010000380         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_464826, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000380 DATNDD010000000
VERSION     DATNDD010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464826"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VN647_09220"
     CDS             complement(<1..930)
                     /locus_tag="VN647_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWG97250.1"
                     /translation="MARLSDLLRERMPQSQSGGALSSREGSSSLNSDRSWCVSTRQEL
                     WKIGEAVRSSKSPQLTRCTDLAAQLVQLLNQGDELVRWALEGQTEDYVLDNALHVAVL
                     GAKVGMGLQYSQQDLERLVLVALVHDVGMWTLPVSLVEKRAALAEEERDILRTHPERG
                     RRILAGQGHVFEWVSTISAQEHERSDGSGYPCCLKDRQIAEPAQIIGMVDTLDAMVTM
                     RPYRKRITPHQGVRELLLHAKTTFSLRVLKALGDQITLYPIGTNVRLNTGEIGTVMRV
                     NSRYPLRPLVAISKLGEASEMDLSQVTSAHIVEV"
     gene            complement(970..1146)
                     /locus_tag="VN647_09225"
     CDS             complement(970..1146)
                     /locus_tag="VN647_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97251.1"
                     /translation="MSKLVRIVVQLPVELKEQLDALKQQGYTTSGFIRATLERELNKL
                     NPRPDPAMRKVVNK"
     gene            complement(1376..>3016)
                     /locus_tag="VN647_09230"
     CDS             complement(1376..>3016)
                     /locus_tag="VN647_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HWG97252.1"
                     /translation="AWPAVALYALLAGAELATMRSLVMITLAMIAMWLGYERHLGHAM
                     AVALLAILLHDPRAIFDISFQLSFLSVLVMIRMIVLTKSWNADAAEADSQMRYRFMSH
                     GLKALSMSGAVTVATLPMVAFYFNQVPWMGAITNLIAVPFTGIILVPLGLLAALWTIM
                     TGAESLILGSAIEQLFGLMVLGLRWCARLPGGEWYVAAPSIPTIALFYTGLLIASFQV
                     IPWRVRVAGAGLSIVLISWWLIPPISQADGDRWRVTFLDVGQGDSAVVELPDGQTVLI
                     DGGARYERFDMGKNVIAPFLWSRRIHHISHVIGTHQQLDHVGGLIWVLRHMSVGWYWG
                     DGIERPEKFAEDLKTVIRDQHIDQHTAVRGQGLLQSGQCRLSILNPPEPSVVRESTQT
                     STGTALNNLSIVLRLQCGTYSILFAADIETAGLRRLDGEGYQPVTVLKVPHHGARSSL
                     DQNWIRQLRPQYAVVSVGAGNPYGHPADSVLQAYQDQHITVFRTDRDGAIWVTGRLST
                     SEFTVSRMRDLIVQPIDFPHCLWRCEYQNWHRLSLQFS"
BASE COUNT          722 a          863 c          768 g          663 t
ORIGIN      
        1 gacctccacg atatgcgcag acgtgacttg cgacaggtcc atctcgcttg cttccccgag
       61 cttagaaatc gccactagag gccgcagcgg gtagcgcgaa ttgaccctca tcaccgttcc
      121 gatttctcct gtattgagac gtacgttggt tccaatcgga tagagggtga tctgatcccc
      181 caacgctttc agcactcgca gagaaaacgt cgtcttggca tggagaagca gttcacggac
      241 tccctggtga ggtgttatgc gcttgcgata gggtctcatc gtgaccatcg catcgagtgt
      301 atcgaccatt ccaatgatct gtgcaggctc ggcgatctgc ctgtccttta aacaacatgg
      361 gtatccgctg ccatccgacc gttcatgctc ctgtgcactg atcgtcgaga cccattcaaa
      421 aacgtgaccc tggccggcga gaatccgacg gcctcgttca ggatgagtac gaaggatatc
      481 tcgttcttcc tcagctaatg ctgctctttt ctcgacaagc gatacaggaa gggtccacat
      541 accgacatcg tgcaccagcg ctaccaacac cagccgctcc aaatcctgtt gtgagtactg
      601 caaccccatc ccgactttgg ctcccaagac tgcgacatgg agcgcattgt ccaggacata
      661 gtcctcggtc tggccctcca aagcccacct gacgagttcg tcgccctgat tgagaagctg
      721 taccaactgt gcggcgagat cggtacatct tgtgagctgc ggacttttac ttgagcgaac
      781 agcctctccg atcttccata gctcctgtct ggtcgagaca caccaggagc gatcactgtt
      841 cagagacgag gatccttccc tcgaagacaa agctcccccg ctttgcgact gaggcatccg
      901 ctctcgaagc aagtcagaaa gccgtgccac ccttgtgttt actcctgtga ttccggatgc
      961 atcatgcctt tacttattca cgacctttct catcgcggga tccggccgtg gattgagctt
     1021 attcagttcc cgctccaagg tggcgcggat gaaaccactc gtcgtatatc cctgttgctt
     1081 gagggcatca agctgctcct tcaactcgac aggaagttga acaacaatgc gaacaagctt
     1141 ggacatgatt gcagctccct tgagacaaag tactaacctg acatgggact tctgcacatg
     1201 ctatgccttg ctggcatctc gttaacggtg cgaaaatact gacacttatg ctgtgctctc
     1261 tcagcatttc tgccgggaag agccaaaaac tgtcatgcat taccggtctt gtacggaaga
     1321 ataccggttg caggaggatg gagcctggac ggccggaaag gaggaatgct tgtcgttatg
     1381 agaattggag ggagagccgg tgccaattct ggtactcaca acgccaaaga caatgcggaa
     1441 agtcaatagg ttgcacaata agatcccgca tgcggctcac cgtaaactcg gatgttgaga
     1501 gccgtcctgt aacccagatc gccccgtcac gatccgtgcg aaagaccgta atgtgctgat
     1561 cttgataagc ttgcagtacc gaatcagcgg gatgtccata gggattaccc gctcccaccg
     1621 agacgactgc atactgtggg cggagttgcc gaatccagtt ctgatccagt gagctacgcg
     1681 ctccgtgatg tggcactttt agtacggtca ccggttggta cccctccccg tctaatcgcc
     1741 tcaatccggc agtctcaata tcagctgcga aaaggatgga ataggttcca cactgcagtc
     1801 gcaagacgat ggacagattg ttcagtgctg ttccggtaga ggtctgagtc gattcacgaa
     1861 ctacagatgg ttccggtgga ttgagaatgc tcagtcggca ctgaccggac tgcaacaggc
     1921 cttggccacg cactgcggtg tgttgatcga tatgttgatc acgaatgacg gtcttgagat
     1981 cttcagcgaa tttctcaggg cgttctattc cgtcgcccca gtaccatccc actgacatat
     2041 gtcggagcac ccaaatcagc ccgccgacgt gatccagctg ctggtgtgtc ccgatgacat
     2101 gggatatatg gtggattctc cggctccaca gaaacggtgc gatcacattt ttccccatgt
     2161 caaatcgttc atatcgagct ccaccgtcga tcagaacggt ctgaccatcc ggcagctcga
     2221 caacagcgct atctccttgt ccgacatcaa gaaaggtaac ccgccaccga tcgccatccg
     2281 cttgcgatat gggagggatc agccaccaac tgatcaacac gatcgacaga ccggcgccgg
     2341 caactcggac ccgccacggt atgacttgaa aacttgctat aagtaacccc gtgtaaaaca
     2401 gggcgatcgt cggtatggat ggtgctgcca cataccactc ccctcccggg aggcgggcac
     2461 accaccgcaa tccaagaacc atcaacccaa ataattgctc tatcgctgag ccaaggatca
     2521 aagactcggc tcccgtcata atcgtccata gggcggcaag caatcccaaa ggtaccaaga
     2581 tgataccggt gaacgggacg gctatcaggt tggtgatggc tcccatccac ggaacctgat
     2641 tgaaatagaa cgcaaccatg ggcaaagtcg ccacagtcac agctccgctc attgacagcg
     2701 ctttgagccc atggctcatg aagcggtatc tcatctgact gtcggcttcg gcagcgtcgg
     2761 cgttccatga tttcgtgagt acgatcattc taatcattac gagcacggac agaaaggaaa
     2821 gttgaaagga gatgtcaaag atggcacgag gatcgtgcaa gagaatcgcc aggagcgcca
     2881 ctgccatcgc atgcccaaga tggcgttcat accctagcca catcgcgatc attgccagag
     2941 tgatcatcac gagcgaacgc atggtcgcca actcggcccc tgcaagcaac gcatacagtg
     3001 cgacagcggg ccaggc
//
LOCUS       DATNDD010000381         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3911648, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000381 DATNDD010000000
VERSION     DATNDD010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911648"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..868)
                     /gene="pal"
                     /locus_tag="VN647_09235"
     CDS             complement(158..868)
                     /gene="pal"
                     /locus_tag="VN647_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWG97253.1"
                     /translation="MKTLPASYLPLILGVIVLGIPACSKKAVRSGGDSQALQEEMAKG
                     GAAKEGTNSSFPDTSFSGREDSNSLRGLDRNPSEERLGGNAGQAGAPGGGHANAMIAK
                     ADSGAAARQLAEIRAEQVASVAAGLRDVFFTYDSFSITDEGRHALSGNAEWARSNPYA
                     QLKIEGHCDERGTSAYNLVLGEKRAKAVRNYLVELGVAPVHLSIVSYGKERPFCTEHT
                     ESCYSQNRRGHLVVKTGK"
     gene            complement(948..>2084)
                     /gene="tolB"
                     /locus_tag="VN647_09240"
     CDS             complement(948..>2084)
                     /gene="tolB"
                     /locus_tag="VN647_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HWG97254.1"
                     /translation="VLKADVRRSLVFALVDLPSLGMKVGRLGAEPDASFKQAAENGVS
                     VIVWGKAGIKNGSKDADISMDGYVYDAGSNEVVGGKRYVGSTSVARLMAHRFADELVF
                     RYTGEPGIARTKIAYVSELGTARELFVMDYDGYDPRQLTADGFLNLMPRWSPDRRFLV
                     FTTYRNRNTQDIDMIELATGKRWTLVSQGGLNITPALSPDGNFLAYSSSHEGNAELYR
                     MDTHKKAVQRLTTHAAGDLSPSWSPSGRELAFTSDRSGGPQIFLMSADGSNVRRLTFD
                     GDYNAAPAWSPRGNWIAYVCRTPKKEYKLCVITPDGQKHLQLTTGLGVDDSPSWSPDG
                     RHLVFSSTVDGKSQIYMINVDGKDLERITFTGTHNSAPSWSPAS"
BASE COUNT          465 a          637 c          518 g          464 t
ORIGIN      
        1 cggactgctg agcgacacac cccggcagaa ggagaccgcc taccgcgata ccgaggagca
       61 taccatgcgt gattcgtcgt tgagacgtca tatgtcctga tctccaaacg accgacgata
      121 atcaggtctc gttcaggcga acggcacgac ccgcgcacta cttccctgtc ttgacgacga
      181 gatgcccgcg gcgattctgc gagtaacacg attccgtatg ttccgtgcag aacggccgtt
      241 ccttgccgta ggaaacgatc gacaaatgaa cgggggccac tcccagctcg acgagataat
      301 tccgaacggc cttcgcacgc ttttcaccca gcactaaatt ataggctgat gtgccacgct
      361 cgtcacaatg cccttcgatt ttcaactgtg cgtatgggtt cgatctggcc cattccgcat
      421 tgccggaaag ggcatgacgt ccttcatcgg tgatcgaaaa actatcgtac gtgaaaaaga
      481 catcccgaag tccggctgcc accgaagcca cttgctccgc gcggatctca gccaactggc
      541 gagctgccgc gccggaatct gcctttgcga tcattgcgtt ggcgtggccg ccacccggag
      601 cacctgcttg accggcattc ccgcccaacc gttcttccga aggattgcga tccaatcccc
      661 gtaagctgtt cgaatcttca cgtccggaga acgacgtatc gggaaaactt gaattcgtcc
      721 cttctttggc cgctcctcct ttggccatct cttcttgtaa agcctgacta tctcctcctg
      781 accgaaccgc cttcttcgag cacgctggaa ttcccagtac gatgacaccg agaatcaacg
      841 gcaagtagct ggctggtaac gttttcattg gattccttcc ctcctgtgaa agatcgtccc
      901 tttttcttac agcgaaggac gattgccgtc aatgccttcc tggatattta cgatgccggg
      961 gaccaggacg gtgcgctatt gtgagtcccg gtaaacgtaa tccgctcgag atctttaccg
     1021 tccacattga tcatgtagat ctgactcttc ccatccaccg tcgagctgaa gacaagatgc
     1081 cgtccatccg gagaccagga cggagaatca tccacaccca gtccggtcgt taattgcaga
     1141 tgtttctgac catcgggggt gatcacgcac agtttgtact ctttcttggg agtccggcac
     1201 acataggcga tccaatttcc tcgaggcgac caggccggcg ccgcattata gtctccatca
     1261 aaggttaatc gacgcacgtt ggatccatcc gcactcataa ggaaaatttg cggtccgccg
     1321 cttcgatcgg atgtaaatgc gagttcccga cccgatggag accatgaggg agacaagtcc
     1381 ccggccgcat gtgtggtcag tcgttggacg gcttttttgt gagtatccat acgatacagc
     1441 tcagcattgc cttcatggct cgacgaatag gcgagaaaat tcccatcagg agagagcgcc
     1501 ggagtgatgt ttagtccgcc ctgtgagacc agggtccagc gtttccccgt cgcgagttcg
     1561 atcatgtcga tatcctgcgt gttccgattg cgatacgtcg tgaagaccaa aaatcgacga
     1621 tccggcgacc aacgaggcat caggttcaag aacccgtcgg ccgtcagctg tcgcggatcg
     1681 tatccatcgt aatccatcac gaataattca cgagccgtcc ccagctccga gacataggcg
     1741 atcttcgttc gtgcgattcc cggttctccc gtatagcgaa agaccaactc atccgcaaaa
     1801 cgatgagcca tgagacgagc caccgatgtc gagccgacat atcgtttccc gcccacgact
     1861 tcattgctgc cggcatcata cacgtaaccg tccatgctga tatcggcgtc cttgctcccg
     1921 ttcttgattc ccgccttacc ccagacaata accgacaccc cgttctcggc ggcctgtttg
     1981 aaggaagcat caggctccgc cccgagacga ccgaccttca tgccaagact cggcaaatcg
     2041 acaagagcga agaccagcga gcgtcgcacg tccgccttga gtac
//
LOCUS       DATNDD010000382         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1107968, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000382 DATNDD010000000
VERSION     DATNDD010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107968"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..849)
                     /gene="larB"
                     /locus_tag="VN647_09245"
     CDS             complement(94..849)
                     /gene="larB"
                     /locus_tag="VN647_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWG97255.1"
                     /translation="MNPEGLERLLRQIRHGQVTVEQALQRLRSLPFEDLGFASLDHHR
                     SLRQGFPEVILCEGKTTAQVIAIARALIRKQGPFLATRADPSVARAIRRLDRRAQYYP
                     DARIVAIRTSRPKQHGHILVVTAGTADVPVAEEARVTAEVMGSDVERLYDVGVAGIHR
                     LLGKKDRLFDAQVVIVVAGMDGVLPSVVGGLVHCPVIAVPTSRGYGASFGGVAALLTM
                     LNSCAAGVGVMNIDNGFGAACLAHRINMLGVKS"
     gene            complement(846..1868)
                     /locus_tag="VN647_09250"
     CDS             complement(846..1868)
                     /locus_tag="VN647_09250"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWG97256.1"
                     /translation="MPTTINKIGVIGAGAWGTALAKHLAEKGLEVRLWAYEQDVVNAI
                     NSSHENSVFLKNVPLPPGLTATSSLVEAVKSRDGVLFAVPSHLTRSVLHQLAPCISGP
                     LPFVCATKGIEENTAKLMTQVMEDELPPSMQRSLMVLSGPSFASEFSAGKPTAVCLAG
                     VDEPLVQRFQRALMTPAFRVYADTDVVGVQLGGALKNVMALAAGVIDGLGLGLNARAA
                     LITRGLAEIIRLGTAMGADPRTFYGLSGIGDLVLTCTGALSRNHSVGVRLGKGERLET
                     ILAGMQAVAEGVRTSRAALTLARHYQVDMPIIQEINAVLYDNKPCRKAVGDLMEREAK
                     PEKGWA"
     gene            2086..2823
                     /locus_tag="VN647_09255"
     CDS             2086..2823
                     /locus_tag="VN647_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWG97257.1"
                     /translation="MKQPYAAWVCSGICTTTFLFSSLLVAVPTAYGETYVAGQFGLAL
                     PGLGGGLSDVDVTSSLFIPDTKHSDLELSNSFMYGGKLGHYFNGARWFGLEAEVFYTT
                     PHIEQQDHTFSHPTVPCPGCTATFQGAHFRVLTVAPFNLMFRYHKTRLQPYLGVGPGI
                     FFARIKGEGLAPDSPESTSDNAKLGLNVKVGLDYYLTKHLSAFAEWKFNYAHFKFNEN
                     QNLWPFFYSFDATYTMHLVSFGIGYHF"
     gene            2912..3283
                     /locus_tag="VN647_09260"
     CDS             2912..3283
                     /locus_tag="VN647_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97258.1"
                     /translation="MQVCPACSRSQPEINRFCIQCGRRLDGRSESPQDSQPRTTSAPH
                     QLNLTVLYGMVVVLILAVLFPPWETPPTQTPEFLGMYFILSPPAPEAVVSRMLLTIEL
                     VTIAIAGMYGAFLFRKKDEGQ"
     gene            complement(3363..>3619)
                     /locus_tag="VN647_09265"
     CDS             complement(3363..>3619)
                     /locus_tag="VN647_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97259.1"
                     /translation="AFDSYGRAAIAERVPDKLRLTVPEDEALKFACNAVCVEKHVVLP
                     TGCPTTQAWLRIRGYETHPVQLDEFMKSGGSAKCLTLALD"
BASE COUNT          799 a         1049 c          937 g          834 t
ORIGIN      
        1 gcaaacggtg agaaagggct gcgcaccgtg atggtcgctt ggccgaagcg gggcatactg
       61 gaagtgaaat agcgtcccct tgtctctctc acgctaactc tttaccccca acatattgat
      121 ccgatgcgcg aggcaggctg cgccgaatcc gttgtcgatg ttcatcactc ccacacccgc
      181 tgcgcaggaa ttgagcatcg tcagcagcgc agcaactccg ccgaaactcg caccataccc
      241 tcggctggtc ggtacggcaa tcacaggaca gtggactaaa cctcccacca cactcggcag
      301 gacgccgtcc ataccggcga cgacgatcac gacctgcgca tcaaacagcc gatctttctt
      361 tccgagcaac cgatggatgc ccgcgacacc gacatcgtac agcctttcga cgtcacttcc
      421 catcacttct gcggtcacac gggcttcttc cgccacaggc acatccgcag ttcccgcggt
      481 aaccacgagt atatgtccat gttgcttcgg cctggaggtg cgaatcgcca caatgcgggc
      541 atcgggatag tactgtgctc gccgatccag acggcgaatg gcacgcgcga ccgatgggtc
      601 ggcacgagtc gccagaaacg gtccctgctt tctgatgagt gcccgagcga tggcgatgac
      661 ctgcgccgtg gtctttcctt cacacaagat cacctcgggg aacccttgtc gcagcgatcg
      721 atgatgatcg agcgaagcaa agcccagatc ctcaaaaggc aatgaccgca gccgctgtag
      781 tgcctgttcc accgtaactt gtccatgacg gatctgccgt agcagccgct cgagtccttc
      841 cggattcatg cccatccttt ctctggtttt gcttcccgct ccatcaaatc accgaccgct
      901 tttcgacagg gtttattatc gtacaggacg gcattgatct cttgtatgat cggcatatcg
      961 acctgatagt gacgggccaa cgtgagtgcc gcacgactcg tccgaacgcc ttctgccaca
     1021 gcctgcattc cggctaatat cgtctccaac cgttctccct tgccgagccg aacgcctacc
     1081 gaatggtttc ggctcagtgc gcccgtacag gtcagtacca gatcgccgat accggaaagt
     1141 ccatagaacg tgcgaggatc ggctcccatt gccgtgccca gccggatgat ttcagccaga
     1201 cctctggtaa tgagcgcggc acgggcattg agcccgaggc ccagaccgtc gatcacaccg
     1261 gctgccagcg ccataacatt ctttaaggca ccgccgagtt gaacgccgac gacgtctgtg
     1321 tccgcgtaca cccgaaatgc aggcgtcatc aacgcacgtt gaaaccgctg caccaatggt
     1381 tcatcgacgc cggcgagaca gacggccgtc ggtttgccgg cactgaattc cgacgcgaaa
     1441 cttggtccgg aaagcaccat gagagaacgc tgcatggacg gcggcaactc gtcctccatg
     1501 acctgggtca tcagtttggc cgtgttttcc tctatgcctt tggttgcaca cacgaacggc
     1561 agagggccgg agatgcaggg tgccaattga tgcaacacgg atcgagtcag gtgcgaagga
     1621 acggcaaaca gaaccccgtc tcggctcttt accgcttcaa cgagcgagga agtggccgtc
     1681 aaacctggag gaagcgggac gtttttgagg aagaccgagt tttcgtgtga ggagttgatg
     1741 gcgttgacga catcctgttc ataggcccag aggcgaacct ccagaccctt ctcggccaga
     1801 tgttttgcca gggccgtgcc ccaggctccg gctccgataa cgccgatctt gttgatcgtg
     1861 gtcggcataa gcgattcagc ctcatggcga aatcatcatt cgtccatttc agaacggcgg
     1921 attataagaa ggcgtcaaag gcctgtcaat gaaggtgggc tatgctgtga acggcaggtc
     1981 actcgttctt ccttcgtcgt caggaattca gaaatagctt cgtcaccatg cgttgaagcc
     2041 ataggatggg agtcgggcac ggatatgggc aattcggagg ctccgatgaa acaaccatat
     2101 gccgcatggg tttgttctgg aatctgtacg acaacgtttt tattctcgtc cctattggtt
     2161 gctgttccaa ctgcatatgg tgaaacatac gtcgccggac aattcggact cgcattgcct
     2221 ggtctaggtg gcggtctctc ggacgtcgat gttacttcgt cactctttat ccccgatacg
     2281 aagcatagcg atctagagct atccaactct tttatgtatg gaggcaaact aggccattac
     2341 ttcaatggtg ctcgctggtt tggattggag gcggaagtgt tttacacgac tccacatatc
     2401 gaacagcagg accatacttt ctctcaccca acagtgccct gtccgggctg tacggcaact
     2461 tttcagggtg cgcattttcg agtattgact gtggcaccct tcaatctgat gtttcgctat
     2521 cacaaaacga gattgcaacc ctatcttgga gtgggaccgg gcattttctt tgcccgaatc
     2581 aagggagaag ggctggcacc agattcccca gaatcaacgt cggacaatgc gaaactcggc
     2641 ttaaacgtca aagttggact tgattattac ctcacgaagc acctttccgc attcgcggaa
     2701 tggaaattca attatgcgca cttcaaattc aacgagaatc aaaatctatg gcctttcttc
     2761 tatagctttg atgcaaccta tacgatgcac ctcgtctcat tcggtatcgg atatcatttc
     2821 taagccgata atcctcagaa caacaccact gggaaaacag gtttctttcc tgtaatctct
     2881 ctctacgttc tggtctctcc ttggcccaat tatgcaagtc tgtcctgcct gtagccggtc
     2941 acaaccggag atcaaccgtt tctgtatcca atgcggtcgg cggctcgacg gtcgatcgga
     3001 gagcccgcag gattctcaac cccgtacgac ctcggcgccg catcaactca atctcacggt
     3061 gctctatggg atggtcgtcg ttctgattct ggccgtatta ttccctccat gggagacccc
     3121 gccgacgcag acgccggagt ttctcggcat gtatttcatc ctctctcctc ccgcgcctga
     3181 ggctgtcgtg agccgcatgc ttctgacgat tgaactcgtc acgattgcga ttgccggcat
     3241 gtatggcgcg tttctcttta gaaaaaaaga tgaaggtcaa tagccatttg catcggtctt
     3301 ttgtaaagag ccaccccgat cctatcgacg agtgaccaat gaccgttgac cgatgacgtc
     3361 gctcagtcca atgccagagt gaggcatttg gccgacccgc cggatttcat gaattcatcg
     3421 agctgaaccg ggtgggtttc ataccctctt atccgaagcc aagcctgggt ggtgggacat
     3481 ccggtaggga ggacgacgtg tttctcgacg cagacggcat tgcaggcgaa cttcaaggct
     3541 tcatcttccg gaacggtgag acgcagctta tccggaacac gttcggcaat cgctgcccga
     3601 ccatagctat caaaagctg
//
LOCUS       DATNDD010000383         5577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2169676, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000383 DATNDD010000000
VERSION     DATNDD010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169676"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VN647_09270"
     CDS             complement(<1..744)
                     /locus_tag="VN647_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWG97260.1"
                     /translation="MPTPTSSPSAETMHDKPGVDRHLWEIRPIRDLFIFGAIAGLLWS
                     LYELHTVFLPVFIAIFFAYLVNPFVDYAFTHWSVPRPLTVGILMLFLTLIFVGLGLWL
                     IPLLIEQAQTLIQKIPVYVRSLSQRYQDLLSNSFNLRDILGRMYDDPLATLQPLFVGT
                     GHAFGLLGQVLGITVNAALYTILIPIYFFFFAWNFERISQTVVRLIPIVHRPHILHVL
                     SRMDTAVRGFVSERLLIAVITAVLYAIGWE"
     gene            990..1160
                     /locus_tag="VN647_09275"
     CDS             990..1160
                     /locus_tag="VN647_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97261.1"
                     /translation="MLYDDRHIRFDETGKIGIARDRLRIIEIVEADMPRSSSRALRAG
                     TARPVHDRYKRR"
     gene            1811..2593
                     /gene="smbP"
                     /locus_tag="VN647_09280"
     CDS             1811..2593
                     /gene="smbP"
                     /locus_tag="VN647_09280"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HWG97262.1"
                     /translation="MGRTRIVLLAFGIATATSVFMSRGEFVHADQADQLVGVTKATNL
                     IGKQVTNLEGKNLGEIKDLVINWRSGGYIEYAVLSFGGFLGLGDKYFAVPWEVMVLSR
                     DKEQFILNVKEERLKNAPGFDKNNWPDMSRPEWAMVVYQFYDLLPNSSKAVSRSSPIE
                     VSRTGKEEAVVDFKSTVREALNHAVEAESAGKQGKPEILVTHAKKSLDSAKRAQRSGH
                     NERLNEGVYALGEAIEHGQKQQTADATEHMMHAIMKLSQSTD"
     gene            2644..3309
                     /locus_tag="VN647_09285"
     CDS             2644..3309
                     /locus_tag="VN647_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWG97263.1"
                     /translation="MKKSNQLCRTMLIAAIVLTPIWGFAEGSRSQRSGDAMSDPAMNQ
                     SQSSFEKQATDMVGGREGLVGKYDVVPVPRGKLVDEKGAALDQIVKNKKGETLGTIEK
                     LLKDTKTGKIQYAVLELEETKDQLPVQWSQFKQKGGNLTLNASKKDLYPIASSVYSKD
                     MSPEVSQYMDEINKLREQPKPKAEGPGAPGLPFDSVGGFGSSGPPEGPAPGYEGGHPS
                     SKR"
     gene            3349..3909
                     /locus_tag="VN647_09290"
     CDS             3349..3909
                     /locus_tag="VN647_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWG97264.1"
                     /translation="MKANAGFWVGCALISIVLSGCNRYQVIPDHLVKQVNEKLSYEQV
                     KDSPETYRGQIVVWGGEVVSAVREDNRTKIEVLQMPLNKNQVPESSKASSQGRFLALD
                     SRQEIIDPAIFEKGSKVTVIGEIEGKSSKSSDLVSSESPVLVIRDMTLWSNDSMLYPV
                     RPYYGYGGYYGYRPYVFWEGTRVSGS"
     gene            3998..4177
                     /locus_tag="VN647_09295"
     CDS             3998..4177
                     /locus_tag="VN647_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWG97265.1"
                     /translation="MRYVAVATDYDNTLAVDGRVERKTIDALERLIQSGRKLILVTGR
                     LLPDLLDIFPAIGLC"
     gene            4202..>5577
                     /locus_tag="VN647_09300"
     CDS             4202..>5577
                     /locus_tag="VN647_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HWG97266.1"
                     /translation="MLYRPATREHTLLAPPLPAAFVDELRRRQVPELSFGDSIVGTVR
                     PHELTLLEIIRDLGLELQVIFNRDAVMVVPSGVNKASGLAAALQEMGLSSRNVVAIGD
                     AENDHALLNQCEYAVAVANAVPMLKEIADWVTTGAAGRGVVQVIDELVAHDLSVSPFS
                     ASHRNILIGLRETGEEVSIPAIGLNLLLAGSSGSGKSTLATGILERVTEQGYQFCVID
                     PEGDYEGFAGAVMFGTSQRGPGMTEILTALESPDTNLVVNLVGLPLQDRPAFFLALLP
                     ALQERRAKSGRPHCILVDETHHLLPRDWQPVQAVLAKDLTGMMYVTVHPDHVAKSVLH
                     TVDVIVALGDDPAGTIAGYCGTLGLPLPEVEPAALDHGEAVFWDRRSTELPCTLRIAP
                     CEADRKRHRRKYAEGELPENRSFFFRGPDNLLNLRAHNLILFMHLAEGVDEKTWLHHL
                     RRGDYSKW"
BASE COUNT         1320 a         1372 c         1689 g         1196 t
ORIGIN      
        1 ctcccatcct attgcgtaca atacagcggt gatcactgca ataagaagac gttcggaaac
       61 gaagccacgc acggccgtat ccattcggct caagacatgc agaatgtgcg gtcgatgcac
      121 gataggaatc aggcggacga cggtctgact aatgcgttca aaattccaag cgaagaagaa
      181 aaagtagatg gggatcaaga tcgtatagag cgcggcattg actgtgatcc cgagcacctg
      241 tcccagcagg ccgaatgcgt gaccggtccc aacgaaaaga ggttggaggg ttgccagcgg
      301 gtcgtcgtac atccgcccaa ggatgtcgcg taaattgaag gaattactca gtaggtcctg
      361 atagcgttgt gaaagactcc gtacatatac cggaattttc tgtatcaatg tctgagcttg
      421 ttcgatcaag agggggatga gccacaatcc gaggccgacg aagatgagag tcaagaacag
      481 catgagtatg ccgaccgtca aggggcgagg caccgaccaa tgtgtgaacg catagtcgac
      541 aaacggatta accagatagg cgaagaaaat ggcgataaac acaggcagga agacagtatg
      601 caattcgtag agagaccaca gcagcccggc gatggcgccg aagatgaaca ggtcgcgaat
      661 ggggcggatc tcccataagt gtcggtcgac tcctggcttg tcatgcatgg tttcggccga
      721 tggtgaggac gtcggtgtcg gcacgtgtcc tggcgtcggc gctgggcggg gcgaactcat
      781 cgaatcacag catggtcagg cgcgattggg gcacagaatc gtatcatcct ccgcccttgc
      841 aaacagcccg acaccagttc atcaaaacga caataacaat aagatggagg ggttacaaga
      901 tagatagaag cgtactcgga tcacatgtca cccgcgaatg cgatggtgct gagcaaggcg
      961 ttttccgcct gaagcgaatc gaggcgggta tgctgtacga cgatcggcac atccgattcg
     1021 atgagaccgg caaaatcggt atcgcgcggg atcggctccg gatcattgag atcgttgaag
     1081 cggacatgcc tcgttcgtcg tcccgggcac tacgagctgg tacggcccgg ccggttcacg
     1141 atcgctataa aagacggtga tgcgcaccag ggcgtcccga tcggatgcat tcaacagaca
     1201 cactgtttca tggctggtca tctgcggcga ggggccatgg cttgcccatg ggaatatagc
     1261 cctctgcaat ggcccatcgt ctgcgtccga tactttccat ttggcaaccg cccggctaaa
     1321 aagactgcaa gcgaggtgcc tagtaagggt agcagtggtc attacggcag gacttcgccc
     1381 gcctacgcag gaagccacgg ttgaccctcc tcagcagtgg tctgctgcgg aggatggatc
     1441 aagcggtggg gctccactga ctgcatcttt gaaccgggct ttcttggaag acttgtctca
     1501 gaatagacat cttgtccgaa gtatgtcgtc catgctgttc ttctctgggt ggtcggcgaa
     1561 gcatccagcg gtagattgaa gggatgcacc tttaggcgga agggcaggaa gaaaaatgaa
     1621 tggccacaag cggcctatgt tttgcagatg tgttttttat gccgagtcgt ttccatcgag
     1681 agcgctctgt tgaccaaacg gtcatcgctc tccggacgat atgtcggaaa cagtccaaag
     1741 atgaccgttg tgattgtacg ggaggatgga atagttgacg gaaggtgcat gtctttaaag
     1801 gaggcgccta atgggaagga cacgaatcgt gctgctcgca ttcggcattg ccacggcgac
     1861 gtcggttttc atgtcgcggg gggaattcgt ccatgcagac caagcagacc aattggtggg
     1921 agtgaccaag gcgacgaacc tgatcggtaa gcaagtgacg aatttggagg ggaagaatct
     1981 cggcgagatt aaggacttgg tcatcaattg gcgaagcggt ggctatattg aatatgccgt
     2041 cctttccttc ggcgggtttc tcggtctcgg tgacaaatac tttgccgttc cgtgggaggt
     2101 catggtgctg agccgcgaca aggagcagtt cattctgaat gtgaaggaag agcgactaaa
     2161 aaatgcacct gggttcgaca aaaacaactg gccagacatg tcgcgccccg aatgggcgat
     2221 ggtggtctat caattctacg atttgcttcc gaatagctcg aaggctgtat cccgttcctc
     2281 gcccatcgaa gtatcccgga cgggaaaaga ggaggccgtc gtggatttca agagtacggt
     2341 tcgcgaagcg ttaaaccatg ccgtggaagc tgagtcggct ggaaaacaag gcaaaccgga
     2401 gatcttggtg acccatgcca agaaatcact cgattcggcc aagcgtgcgc aacggagcgg
     2461 ccacaatgag cggctcaatg aaggagtgta tgcgctgggc gaagcgatcg aacatgggca
     2521 gaaacaacag acagcggatg ccaccgaaca catgatgcac gccattatga agctttcaca
     2581 gtccaccgat tgaacacccg gaatccatat tgcccataca tgctatccag attggaggtc
     2641 gccatgaaga aaagcaatca gttgtgccgt acgatgctca ttgccgcgat agtgttgaca
     2701 ccgatttggg gcttcgccga agggagtcgt agccaacgat ccggtgatgc gatgtccgac
     2761 ccggccatga atcagtcgca atcgtccttc gagaaacagg cgaccgatat ggtcggcggc
     2821 cgggaggggc ttgtcgggaa atatgacgtt gtgccggttc ctcgcggtaa gttggtcgac
     2881 gaaaagggag cggcgctgga ccaaatcgtt aaaaataaga agggcgaaac cttgggcacc
     2941 atcgagaagt tgctgaaaga caccaagaca ggaaagatcc agtacgcagt ccttgaattg
     3001 gaagagacaa aagatcagct gcccgtccaa tggagtcagt tcaaacagaa agggggcaat
     3061 ctgacgttga atgcctccaa gaaagatttg tatccaatcg cgagttccgt ctattccaag
     3121 gatatgtcac cggaggtctc ccagtacatg gatgagatca acaagctccg cgaacaaccg
     3181 aagccgaagg cggaaggtcc gggtgccccg ggccttccgt ttgactcggt gggtggtttt
     3241 ggatcgtcag gccccccgga gggtccggct cccggttatg aaggcggtca cccaagcagc
     3301 aaacggtaac tgagcttggc aaggctgata tatgaagaga ggtgttctat gaaagccaat
     3361 gctgggtttt gggtcggatg tgcgctcatc tcgattgtcc tgagcggatg taaccggtat
     3421 caggtcatcc cggatcatct ggtgaaacaa gtcaacgaaa agttgtcgta tgagcaggtc
     3481 aaagattcac cggaaaccta tcgagggcag attgtcgtgt ggggcggaga agtggtcagt
     3541 gccgtccgcg aagacaatcg gacgaaaatc gaggtgctgc aaatgccgtt gaacaagaat
     3601 caggttcctg agtccagcaa agcgtcgtcg cagggacgat ttttggcgtt ggacagtcgg
     3661 caggagatca tcgatccggc aatttttgaa aagggatcga aagtcaccgt catcggcgag
     3721 attgagggga aatcctcaaa atcgtccgac ctcgtgtcat ctgaatcgcc tgtcttggtc
     3781 attcgcgaca tgacgctgtg gagtaacgat tcaatgctct atccggtccg cccttactac
     3841 gggtatggag ggtactatgg ttatcggccg tatgttttct gggaagggac tcgtgtgtcg
     3901 ggaagttaag cccgctgaga cccggtggga tgccgtattt tcgatcccat gatagctcta
     3961 tcaagacgaa gggcacttgt cattcggcat acatcctatg agatatgtcg ccgtagcgac
     4021 ggattacgac aacacgttgg ccgtcgatgg ccgggttgaa cgcaagacga tcgacgctct
     4081 tgagcgcctc attcaatcgg gacggaagct cattttggtc acagggcgtc tgttgccgga
     4141 tctcttggac atctttcccg caatcggtct gtgctaacga gtcgtcgtag acaacggtgc
     4201 tgtgctctac cgccccgcga cacgcgaaca cacgctgttg gcgccgccgc ttcccgcggc
     4261 gttcgtcgac gagctgcgcc gccgacaggt cccggagctt tcgttcggag attccatcgt
     4321 gggcacggtg cggccgcatg agctgacgct gctcgagatc attcgagatc ttgggctcga
     4381 actacaagtc attttcaacc gggacgccgt catggttgtt ccgtccgggg tgaacaaggc
     4441 cagtggtctt gcggcggcat tgcaagagat gggcttatcg tcgcgcaatg tcgtggcgat
     4501 cggagatgcg gaaaatgatc atgccttgct caaccagtgt gaatatgccg tagcggtagc
     4561 gaatgccgtg ccgatgctca aggaaatcgc cgattgggtc accaccggtg cggctgggcg
     4621 gggagtcgtc caagtgatcg atgaacttgt ggcgcatgat ctctctgtgt ctccgttcag
     4681 cgcgagccat cgcaatattc tgatcggtct gcgcgagacc ggtgaggagg tgtcgattcc
     4741 cgccatagga ctcaacttat tgctggcggg ttcgtcggga agcggcaaat cgacgttggc
     4801 caccgggatt cttgagcggg tgacagaaca agggtatcag ttttgtgtga tcgatcctga
     4861 aggagactac gaggggtttg caggagcggt catgttcggg acttcgcaac ggggaccggg
     4921 aatgacggag attctcaccg ctctggaaag cccggacacg aatttggtcg tcaatctcgt
     4981 gggcttgccg ttacaggatc gtccggcatt ttttctggcc ctcttgccgg cccttcagga
     5041 gcgtcgggcc aaatccggtc gaccgcattg tatcctcgtc gacgaaacgc atcatttact
     5101 gcctcgtgac tggcaaccgg tgcaagctgt gctggcgaaa gatttgaccg gtatgatgta
     5161 tgtcaccgtc catcccgacc atgtcgcaaa atccgtgctt catacggtgg atgtcatagt
     5221 ggccttagga gatgacccgg cgggtacgat tgcgggatat tgtggaacgc tgggcctgcc
     5281 gttgccggag gtcgaacctg cggcgctgga tcacggtgaa gcggtctttt gggatcggcg
     5341 gtccaccgag ttgccctgca ccttgcgcat cgccccttgt gaagcggacc gtaagcgtca
     5401 ccgccggaaa tacgcggaag gcgaattgcc ggaaaaccga agcttttttt tccgtggtcc
     5461 ggacaatctg ttgaacttac gagcgcacaa cctcattctc ttcatgcatc tcgcggaggg
     5521 tgtcgatgag aagacatggt tgcatcacct tcggcgaggg gattatagca aatggat
//
LOCUS       DATNDD010000384         4655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3609051, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000384 DATNDD010000000
VERSION     DATNDD010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609051"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VN647_09305"
     CDS             complement(<1..484)
                     /locus_tag="VN647_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97267.1"
                     /translation="MSMAPPSVAVSDARVTKHAIAPSRGGRPWTFYVLAGLFTFYVIA
                     LYGPMICIYILSFQDVRGGLVFPMKGLSLHWFVDLFTQVRTGDVKGAFDRSIKLAIVV
                     TIITVVVSFMSAMAFRRRFRGDNIVFALMIGSLVAPGLVLGIGVGLLFNAIGIDTSPY
                     T"
     gene            complement(481..1344)
                     /locus_tag="VN647_09310"
     CDS             complement(481..1344)
                     /locus_tag="VN647_09310"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG97268.1"
                     /translation="MAVSRSMMSWLYISPLMLVLIPFFVLPILVVLVASVFQTDGFGG
                     LIPTFTLTNYGTVLTSGLTYNLYFETVKFTVLTWFFTLLLGFFVAYFLVFHVRNPLLA
                     IGLFLICTVPFWTSNIIRMISWIPLLGKEGLINSALLHLGLIREPLEFLLYSGFAVVV
                     AYVHQLTIFMIVPIFNSMARIDKRVVEAAIDAGASRFDIMRLIIVPLSKSGIALGSIF
                     VVSIVMGDFFVTKVMSGGGSASVVGAFYEDISVLQYPSAAASAIILTVILLVMVSVIL
                     RSVDIRKEITQ"
     gene            complement(1423..2733)
                     /locus_tag="VN647_09315"
     CDS             complement(1423..2733)
                     /locus_tag="VN647_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWG97269.1"
                     /translation="MTSKGKLKGGLSRRSVLKAGAAVAGAAVGTEAITGFPTIWAQDI
                     KDIELRHVGVSYSVVKAIGDQAAKDLGFKVTMQNLDTSAAINRFITQPNSVDIADLEG
                     WQAKLAAKRGVIQGIEAKKIKEFDNFLPIFTKGELDGHKIPRQGISPYEALYIEKPDS
                     TELHDGVTEWLTNVPQVYNADSIGYRPDLVGHKVTEWKELIDPKFKGKAAILDVPAIG
                     IMDAALCFESAGLIKYGNKGNMTKKEIDFTCDKLIELKRAGQFRATWTTFDQSVQLMA
                     AGEVVIQSMWSPAVAAVRVKQIPCVYAPVNIKDGKEGYRGWCNGMGLMKHLTGKKLDA
                     AYEYMNWYMSGWQGAFVSRYGYYSPVPSTARKFMTPAEYTFWYEGKPAPEPVTDPYGV
                     PMEVAGTVRDGGSFVERVTNISCWNTLMDEAAYMNKRWNDFKVA"
     gene            complement(3217..>4655)
                     /gene="hydA"
                     /locus_tag="VN647_09320"
     CDS             complement(3217..>4655)
                     /gene="hydA"
                     /locus_tag="VN647_09320"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWG97270.1"
                     /translation="DEQMSDFDLVIRGGTVVTAADTVKADVGIRSGQISSVAAKIEGG
                     RKEIDASGMWVMPGGIDSHVHLSQPAFGGPAMADNFESGTRSAIAGGNTTVLPFALQP
                     RGASLRASVVDYHKEADGKCYTDYGFHLIISDPTASVLGQELPALVADGYTSFKVFMT
                     YDDLVLNDRQLLEVFDCARECRALVMVHCEGYDAIRFMTDRLEQAGKTAPYYHAVSRP
                     ASVEREATHRAISHAELTDVPIMIVHVSGREPMEQIRWAQHKGLKIYGETCPQYVALT
                     ADDLKGLNMDETGGKYVCSPPPRDKASWDAIWEGIRTGVFQTFSSDHCPFTYGSVQGK
                     LNPKARTSFRWVPNGIPGVETRLPILYSKGVAEGRITLNEFVALTSTNHAKMYGLYPK
                     KGSIAPGFDADIVIWDPNRKEVIRQEIMHHGADYTPYEGLAVTGWPIMTILRGKIAME
                     EGKILGAPGDGAFLKRDLSPYAQSRRST"
BASE COUNT         1057 a         1382 c         1248 g          968 t
ORIGIN      
        1 aggtgtaggg agatgtgtcg atgccgatcg cattgaatag aagcccgacc ccgatgccca
       61 gtacgaggcc cggcgccacc aggctaccga tcatcagcgc gaacacgatg ttgtcccccc
      121 gaaaacgccg gcggaacgcc atcgcagaca tgaacgagac caccaccgtg atgatcgtca
      181 ccacgatcgc gagtttgatc gagcggtcaa aggcaccctt gacgtcgccg gtcctcacct
      241 gcgtgaatag atcgacgaac cagtgcagcg acagcccctt catcgggaac accaggcccc
      301 cccgaacatc ctgaaacgac aggatgtaga tgcaaatcat cggaccatag agcgcgatca
      361 cgtagaatgt gaaaagtccg gccagcacat aaaaggtcca tggccggccg ccgcggcttg
      421 gcgcgatcgc gtgtttggtc acgcgcgcgt cagaaactgc gacactcggg ggcgccatgc
      481 tcattgggtg atttcctttc ggatatcgac gctacgcagg atcacggaaa ccatcaccag
      541 cagtatgacc gtgaggataa tcgcactcgc cgccgcgctt ggatactgca gcaccgaaat
      601 gtcctcgtag aaagcgccta ccaccgacgc cgagccacca ccggacatca ccttggtgac
      661 gaagaaatca cccatcacga tcgacaccac gaagatcgag ccgagcgcga taccgctctt
      721 ggaaagcggc acgatgatca gccgcatgat atcgaaacgg ctcgcgccgg catcgatcgc
      781 cgcctcgacc acgcgcttgt cgatgcgcgc catcgaattg aagatcggca cgatcatgaa
      841 tatcgtgagc tggtgcacat aggcgacgac gactgcaaat ccggaataaa gcaggaattc
      901 gagcggctcg cggatcaggc ccagatgcag gagagccgaa ttgatcaggc cttcctttcc
      961 caagagtgga atccaggaaa tcatccggat gatgttggac gtccagaacg gcacggtaca
     1021 gatcagaaat agcccgatcg cgagcaacgg attccgcacg tggaagacca ggaaataagc
     1081 cacgaaaaaa ccgagcagca acgtgaagaa ccaggtgagg acggtgaatt tcacggtttc
     1141 gaaatagaga ttgtacgtca gacccgacgt caacaccgtt ccgtagttgg tcaaagtgaa
     1201 ggtcgggatc agaccgccga atccatcggt ttggaagacg ctcgcgacca gcaccaccag
     1261 aatcggcaac acgaagaacg ggataagcac gagcatcagc ggcgaaatat aaagccagct
     1321 catcatcgat cgggaaacag ccatgaacaa ctctctgcag acatccaaaa ccccgccggt
     1381 cggccgaagg gtcggccggc aggttgttga tggcggctgg acttaagcga ccttgaagtc
     1441 gttccagcgc ttgttcatgt aggccgcctc atccatcagc gtattccagc acgagatatt
     1501 ggtgacgcgc tcgacaaacg agccgccgtc gcggacggtc ccggccactt ccatcggcac
     1561 gccgtacgga tcggtcacag gctcgggcgc cggttttcct tcataccaga acgtgtattc
     1621 ggctggcgtc atgaatttcc tggcggtcga aggcaccgga ctatagtagc cgtagcggct
     1681 cacaaacgcc ccctgccagc cggacatgta ccagttcatg tattcgtagg cggcatcgag
     1741 cttcttgccg gtcagatgct tcatcaggcc catgccgttg caccagccgc gatagccttc
     1801 cttgccatcc ttaatgttga ccggggcata aacgcaagga atctgcttga cccgaaccgc
     1861 ggcgacggcc ggcgaccaca tggactggat caccacttca ccggccgcca tcaattgcac
     1921 cgactggtcg aacgtggtcc acgtcgctcg gaattggccg gctctcttca gttcaatcaa
     1981 cttgtcgcaa gtgaagtcga tctccttctt ggtcatgttg cctttgttgc cgtatttgat
     2041 cagcccggca ctttcaaaac acaaagccgc atccatgatg ccgattgccg gcacatcgag
     2101 gatcgccgcc ttccctttga atttgggatc gatgagttcc ttccattccg taaccttgtg
     2161 tccgaccaga tccggacggt agccgatcga gtcggcgttg tagacctgcg gaacgttggt
     2221 caaccactcg gtgacgccat cgtgcagttc ggtggaatcg ggcttttcga tataaagcgc
     2281 ttcgtaaggc gagatgcctt gacgcggaat cttgtggccg tcgagttcgc ccttggtgaa
     2341 gatcggaaga aaattgtcga attccttgat cttcttggct tcgataccct ggatcacgcc
     2401 gcgcttggcc gcaagcttgg cctgccagcc ttcgaggtcc gcgatgtcca cactgtttgg
     2461 ctgggtgatg aagcggttga tcgcggcaga agtatcaagg ttctgcatcg ttaccttgaa
     2521 accgagatcc ttggccgcct gatcgccgat cgctttcacg accgaatacg acacgccgac
     2581 atggcgcagt tcgatgtcct tgatgtcctg cgcccaaatc gttggaaagc ccgtaatggc
     2641 ctccgttccg accgccgctc ccgcaaccgc ggcccccgct ttcagaacgg atcgacggct
     2701 caggccgccc ttcaacttac ctttcgaagt catacgtgag tcctccatct gatcttcagt
     2761 cgttgtctcg tgaagtctgg cagcaactct ccacccacgc ccggagcaat aaactccgtc
     2821 gtgttgtaag ccccgagggc atatgaattt cgctttgcta atggccggcg cgcttgtccg
     2881 ccaattgccc cgttttgcca agtctacggg cgcagacttc gaccgatagt agtcatttaa
     2941 ctagggtcga cacttggcat gctacttttc acgccactaa cggacgcaaa attgtgcgcc
     3001 gatgtcagct ttctcgccaa cgcaaaaagc gaaagcccgt tgcggagaag tgggccttcc
     3061 aggcaaaagc gtgatggagg atctcccgct aagcccaaca acgagtcacg catgaacctc
     3121 atgggcgttg cgccggaacg ctcagccatc cacaacggct cggcaaaggc gtccggagtc
     3181 gaggcgaggt cttcaaagct tatggaattc tcttcgctat gtagaacggc ggctctgcgc
     3241 atagggcgac aaatcgcgct tgaggaacgc accgtcccca ggagcgccaa gaatttttcc
     3301 ttcctccatt gcgatcttgc cacgcagtat tgtcatgatc ggccaacccg ttacggccaa
     3361 cccttcgtaa ggagtgtagt cggcgccgtg atgcattatt tcctgacgga tcacttcttt
     3421 acggtttgga tcccagatga cgatatccgc gtcgaatccc ggagcgatcg atcctttttt
     3481 tggataaagc ccgtacatct tcgcgtgatt tgtcgaggtc agagccacaa attcgttcag
     3541 ggtaatgcgt ccctcggcta cccccttgga gtaaagaatt ggaagtcgag tctcaacgcc
     3601 gggtattcca ttaggcaccc aacgaaacga cgtgcgggcc ttgggattta gctttccttg
     3661 gacgctgcca taggtgaaag ggcaatgatc ggacgagaat gtctggaaaa cgccggtccg
     3721 gattccttcc catatggcat cccagctcgc tttgtcccgc ggaggcggtg aacaaacgta
     3781 cttgcctcca gtctcatcca tattgaggcc ctttagatcg tctgctgtca gggccacata
     3841 ttgcgggcat gtttccccat aaattttgag acccttgtgc tgtgcccacc ggatctgttc
     3901 catgggttca cgtccggaca cgtggacgat catgatcggg acatcggtca gctcagcatg
     3961 actgatagca cgatgggtgg cctctcgttc caccgaagcg ggtcgcgaaa ccgcgtgata
     4021 gtaaggcgca gtcttgccag cctgctcgag gcgatccgtc atgaagcgga tggcgtcgta
     4081 tccttcacag tgcaccatca ccagtgcccg acattcccgc gcgcagtcga acacttccag
     4141 gagctgccta tcgttaagta caaggtcgtc ataagtcatg aacaccttga acgatgtgta
     4201 tccatcagcg accagggcgg gcaattcctg accgagaacg ctggcggtcg gatcagaaat
     4261 gatcaggtgg aaaccgtaat ccgtataaca tttgccgtcc gcctccttgt gatagtccac
     4321 aacgctggca cggagcgacg ctccgcgcgg ctgaagtgca aatggaagta cggtcgtatt
     4381 tcctccggcg atcgctgaac gcgtcccgct ttcgaaatta tcggccatgg cgggtccacc
     4441 gaacgccggc tgcgaaagat gaacgtggct gtcaatacct cccggcatca cccacatacc
     4501 cgatgcatca atctcctttc gaccaccttc gatcttcgcg gcgacagatg agatttgccc
     4561 ggagcgtatg cccacatctg ccttgaccgt atcggctgcc gtaacaaccg tgccccctcg
     4621 aatgactaag tcaaaatcgc tcatctgctc gtcct
//
LOCUS       DATNDD010000385         4371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3053228, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000385 DATNDD010000000
VERSION     DATNDD010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053228"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VN647_09325"
     CDS             <1..239
                     /locus_tag="VN647_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWG97271.1"
                     /translation="MHMNVTWQSRKLEWLFVTPRYHRVHHLKDFGKAGANFGVLFTVW
                     DRLFRTYVDPEQVGLTGPYGISEEVHPIRMAVGV"
     gene            complement(541..969)
                     /locus_tag="VN647_09330"
     CDS             complement(541..969)
                     /locus_tag="VN647_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWG97272.1"
                     /translation="MVFLDVNVLVYAHREDAPHHREHLAWLEALIRSEQPYGLSDLVL
                     SGFLRIVTHPSVFNPPSSMEKGLAFTQELRDQPNCALINPGPRHWEIFSKLCRTGGIK
                     GNLVPDAFLAALAIESGSEWISTDRDYHRFPGLRVRHPLE"
     gene            complement(956..1207)
                     /locus_tag="VN647_09335"
     CDS             complement(956..1207)
                     /locus_tag="VN647_09335"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HWG97273.1"
                     /translation="MRTTIRLDDQLLRSAKRFAHETGQSLTKLIEDALRHALARRTAK
                     PARKPIKLTTVSGYGLRSGVDIDDSAALLAFMERPHGFS"
     gene            complement(1411..2052)
                     /locus_tag="VN647_09340"
     CDS             complement(1411..2052)
                     /locus_tag="VN647_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HWG97274.1"
                     /translation="MRHPARSAAQFIPAKLTIPNLQQAAASCTGCDLYRRATQIVFGE
                     GSTHAKVMFIGEQPGDQEDRAGHPFVGPAGKILDKALVEVGITRESVYVTNAVKHFKW
                     EPQGKRRKHKKPSASEVAACRPWLEAEVQVVKPRIVACLGVTAAQSVFGKAVRLNDLR
                     GRPWSTPLASNVFVTVHPSAILRHPDAAQREEDYHRFVKDLRQIKQFLQAQAA"
     gene            complement(2252..2392)
                     /locus_tag="VN647_09345"
     CDS             complement(2252..2392)
                     /locus_tag="VN647_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97275.1"
                     /translation="MPASQMHRDVGRRIIMVGVLITEKSAETKHGQAMEFITLEDVTA
                     LL"
     gene            2872..3219
                     /gene="rpsF"
                     /locus_tag="VN647_09350"
     CDS             2872..3219
                     /gene="rpsF"
                     /locus_tag="VN647_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWG97276.1"
                     /translation="MELYESLFIIRPSVSDEETKSLIDKMKNVADKTGAQFIKAENLG
                     KKKLAYEVRRERKGTYAYFYFKAPNNTVGELERAYRLEDNIIKFLTVHHEKPLMERRP
                     VETSAQESDGGRI"
     gene            3206..3604
                     /locus_tag="VN647_09355"
     CDS             3206..3604
                     /locus_tag="VN647_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWG97277.1"
                     /translation="MAGFNKVILMGNLTRNPELRYTPSGTPVASFGLAVSRRFKQGDD
                     LKEEVCFVDIVVFGRQAEHCGQYLSKGNGAIVEGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFMPKRQEGGTGAEPPMHDDPGYDMGEEG"
     gene            3632..3880
                     /gene="rpsR"
                     /locus_tag="VN647_09360"
     CDS             3632..3880
                     /gene="rpsR"
                     /locus_tag="VN647_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWG97278.1"
                     /translation="MDRSDNERGGGRLFQRRRPCRFCLEKAPIDFKDAGLLRNFLTER
                     GRIVPRRISGNCMRHQRELTVAVKRARHIAIISFAEER"
     gene            3943..>4371
                     /locus_tag="VN647_09365"
     CDS             3943..>4371
                     /locus_tag="VN647_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWG97279.1"
                     /translation="MDVLTPKIADITAEGLSLSGDLTGEELGLTDTDVSIHGTLAVGL
                     DLRAIERTIYVTGVVEGTAVRQCVRCLKEFDDPLAFSLRVAYEREVKAMGAVTKRDDP
                     RKRKSAAPPEGEVEEQNDDLYYFAGDHLELAPMLREQLILA"
BASE COUNT         1031 a         1096 c         1291 g          953 t
ORIGIN      
        1 ggatgcacat gaacgtgacc tggcagtcaa gaaaactgga gtggctcttc gtcacgcctc
       61 gctatcatcg tgtgcaccac ttgaaagact ttgggaaggc gggagcaaat ttcggggtgc
      121 tgtttaccgt gtgggatcgg ctgttcagga cgtatgtcga tcctgagcaa gtgggtctga
      181 cagggcccta tggaatatca gaagaagtcc atcccatccg catggcagtc ggggtctaag
      241 gggagaagcg atcttaatca accatgactc gccgccgtgt tcagtaaacg gtcaatagaa
      301 aacagtcagg cccccatttt tacatggata ctagggcaca caaggctgat accgcaataa
      361 cagatctttg agatgcagta ctgcaatttt aaaaaaaatt gaaatagggt caaaaaaaat
      421 gaaatagggt caagtcttgc ctcttgcatt tctgttgcga accataggcg catcgcccac
      481 acctcaaaca tgcactgaat agtggttgtt tttaatcgcc atgagtatgg gctggatgcg
      541 ttattccaaa ggatggcgca cacgcaggcc ggggaatcga tggtaatcgc gatccgtcga
      601 aatccattca ctcccacttt cgatcgctaa agcagccaga aacgcatcgg ggacgagatt
      661 acctttgata ccgccggttc ggcagagctt gctgaagatt tcccaatggc ggggaccagg
      721 attgatgagg gcgcagttcg gctgatcccg gagttcctgg gtaaaagcca gtcccttctc
      781 catactgctg gggggattga acacactggg atgcgtcacg atgcgaagaa aaccgcttaa
      841 aacaagatca gatagtccat atggctgctc tgatcgaatc agtgcttcga gccatgccag
      901 gtgctctcga tggtggggcg catcttcgcg gtgggcatag accaatacat taacgtcaag
      961 aaaaaccatg aggtcgctcc atgaaggcga ggagagcggc agaatcgtcg atgtccacgc
     1021 cggagcggag gccgtacccg gatacggtcg tgagttttat cggtttgcgc gctggtttgg
     1081 ccgtgcgcct cgcgagcgca tgtcgcagag catcttcaat cagcttcgtc aacgactgac
     1141 cggtctcatg ggcaaagcgt ttggccgatc tgagaagttg gtcatcgagg cgaatcgtgg
     1201 tccgcatatg tcagagcata tgctcatgac atcaatatgt caacttggaa ttggtttgat
     1261 ggcagtgcgt gggaagtggt gccgtcccac gttgcggttg ccttcgatgt aacgctgaag
     1321 gacatacatt tcttggccga tgatcccttg ctccttatga atcagtgacg aatatggaaa
     1381 gtcactgccg gcagcttttg cggtagagcg ttacgcagct tgtgcttgca ggaattgttt
     1441 gatctgtcga agatctttga cgaagcggtg atagtcttct tcacgctgcg ctgcgtccgg
     1501 atgtcgaaga attgccgatg ggtgtacggt cacaaagacg ttcgaagcga ggggcgtgct
     1561 ccaaggccgt cctcgcaggt cattcaaacg aacagccttg ccgaaaaccg actgagctgc
     1621 cgtcacgccg agacacgcga caattcgtgg cttgaccact tgcacttccg cttcgagcca
     1681 ggggcgacag gcggcgactt cggaggccga cggcttcttg tgtttacgac gcttgccttg
     1741 cggctcccat ttgaaatgtt tcacggcatt cgtgacataa acgctctctc gcgtaattcc
     1801 cacttcaacc aaagccttgt ccaagatttt accggctggt ccgacaaagg gatgtccggc
     1861 ccggtcctcc tggtcgccag gctgctcgcc gatgaacatg accttcgcat gggttgaacc
     1921 ttctccaaac acgatttgcg tggctcgtcg gtacagatcg catccggtac aggaagcggc
     1981 cgcttgttgc aaattgggga tagtcagttt agcaggaatg aattgggcgg ctgatcgggc
     2041 agggtgtcgc atgactcacc atacaccggt tctcttacga ggggcagcgg gtgatttccc
     2101 tcgtccgccc tcgattccaa tagatgaaga tccaggacgg tcggcgtcac gacaccgaac
     2161 gtttcttcca ccagtccatg gaccatatat gaccggtttg aggagaacag atgacagcag
     2221 cggccgatag acctcaggga acaacatggc atcataaaag ggctgtgacg tcttccaggg
     2281 tgatgaattc catggcttgg ccgtgcttcg tctcagcgga tttttccgtg atcagcacac
     2341 cgaccatgat gatgcgccga ccgacgtcgc gatgcatctg ggaggccggc accggtcaga
     2401 gccgttcgat cttcttgcgg taaagggtca agggatgggg ctggcctggg atggctccgg
     2461 ttccacacgc cgtagaaagt cttggaacga ctggtatgga ccattctgtg tgcgttcacc
     2521 gataatttgt tcgtcgagtt ctttcgagat tgtcttcgcc tgcatcaagc ccatccgcag
     2581 ccgctcacct gcccgcgacc gaaccgcgct tacacttcca gtcaagtacg tataaaaacg
     2641 gagcatagcg aggtgaaagc cggtaagtgg ggcgagacca ggcatcccgc agggagaaac
     2701 gatatctagc gcagatggga tctaccgaag ctgggtgaga actgcctcca accccgcttt
     2761 tgttgacagc catttccacc ctgtgctacc gtccaccgtt cttgcgccga ataggcgccc
     2821 tacacctcgc tcccgactgg ttcgggggcc tttgcccagg aggatcgctg catggagctc
     2881 tacgagtctc tgtttattat tcgtccgtcc gtctccgatg aggagaccaa gtcgctcatc
     2941 gacaagatga aaaacgtcgc cgataagacc ggcgcccaat tcatcaaagc cgagaattta
     3001 ggaaagaaaa agctcgccta cgaagtgcgt cgcgagcgga agggtaccta cgcctatttc
     3061 tactttaagg cgccgaacaa cacggttggt gagctcgaac gggcgtatcg attggaagac
     3121 aacatcatca agtttttgac ggtccatcac gagaagccct tgatggaacg gcgtccggtg
     3181 gaaacctcgg cacaggagtc ggacggtggc cggatttaac aaagtcatac tgatggggaa
     3241 cctcacccgt aaccctgagc tccggtatac gccgagcggg acaccggtgg cgagttttgg
     3301 cttggcggtg agccgtcggt ttaaacaggg tgacgatttg aaggaagaag tctgcttcgt
     3361 cgacatcgtg gtattcggca gacaggcgga acattgcggg cagtatctca gtaaaggcaa
     3421 cggggcgatc gtcgaaggtc ggttgcagca gcgccgatgg gagactgagg acggccaaaa
     3481 acgcagcaag cacgaagtgg tcgcgcagac cgtgacgttc atgcctaagc gccaagaggg
     3541 tgggacgggt gcggaacctc cgatgcacga cgaccccggt tatgatatgg gtgaagaagg
     3601 ttaatagcac gagtattatg aggaggcagt catggaccga agcgacaacg aacgtggcgg
     3661 ggggcggtta ttccagcgaa gacgtccctg cagattctgc ctggaaaagg caccgattga
     3721 ctttaaagac gccgggcttc ttcgaaattt tttgacggaa cggggaagga tcgttccgcg
     3781 ccgtatttca ggaaattgca tgcgccatca gcgtgaactg acggtggccg tcaagcgggc
     3841 tcggcatatc gccatcatca gctttgccga ggagcgatag cgaacgtgat cgacgatttc
     3901 gatgcaccga ggtggaacag cggcacgttg ccgagaatga ctatggatgt attgacaccg
     3961 aaaatcgcag acatcacggc agaaggtctt tcattgtcgg gagatctgac gggagaggag
     4021 ttgggactga cggatacgga tgtgtctatt cacggaacgc tggcggtcgg gctggatctt
     4081 cgagcgattg aacgtaccat ctatgtgacc ggagtcgtgg aagggacggc ggttcgccaa
     4141 tgcgtacggt gcctcaaaga atttgacgac ccactggcgt tttcattacg tgtggcctat
     4201 gaacgggaag tcaaagcgat gggtgccgtc acgaagcgag acgatccgag gaagaggaag
     4261 tccgcagcgc ctccggaagg agaagtggaa gaacagaacg acgatctcta ttactttgcg
     4321 ggcgaccatc tggagctggc gccgatgctg agggaacaat tgattcttgc t
//
LOCUS       DATNDD010000386         3258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3056374, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000386 DATNDD010000000
VERSION     DATNDD010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056374"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            845..1414
                     /gene="pyrR"
                     /locus_tag="VN647_09370"
     CDS             845..1414
                     /gene="pyrR"
                     /locus_tag="VN647_09370"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWG97280.1"
                     /translation="MMTTPLNKPVERKDEKLIMDAGDISRAMIRIAHEILERNKGVKD
                     LALVGIRTGGVHLAHRLVKRIHGIEGVQIPIGELDITLYRDDLALRKNQPILRKTSVP
                     FDMTNKVVVLVDDVLFTGRTIRAAMDGLMDLGRPEEIQLAVLIDRGHRQLPIKANYIG
                     KNLPTSRDEQVQVLLDEDGEDDRVVILKS"
     gene            1428..2348
                     /locus_tag="VN647_09375"
     CDS             1428..2348
                     /locus_tag="VN647_09375"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWG97281.1"
                     /translation="MSLKRKYLLSLAPLSVDEIRLVLDTADSFKEVTGREIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLANGVKSSVINAGDGWHEHPTQALLDLYTIRQRGMNFHGLRVA
                     IVGDVSHSRVARSNIYALIKLGAEVRLVGPPTMMPCGVEKLGVKVYSDMDEGLRGANV
                     IMMLRLQLERQGRALFPTIREYSRLYGLTAERVRLADAGAIVMHPGPINRGVEIAPDV
                     ADSLSSVILDQVANGVAVRMGILYLMSGAN"
     gene            2580..>3258
                     /locus_tag="VN647_09380"
     CDS             2580..>3258
                     /locus_tag="VN647_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWG97282.1"
                     /translation="MSILIKGGLVIDPGRFVGIADVLIDKGKIDAVRPNLSAPVDGRI
                     IQANGKLVMPGFVDLHVHFREPGFEYKETIQSGSAAAVAGGFTTVCCMTNTNPVNDNQ
                     AVTEFILERARLAGLANVLPVGAITKGSEGKELAEIGDLCRSGCVAISDDGKPVMNSL
                     VMRRAMEYALAFDLTVVDHCEDLHLAEGGCMNEGLVSTELGLPGIPAAAEDVMVARNL
                     SLSELTGA"
BASE COUNT          728 a          822 c          959 g          749 t
ORIGIN      
        1 cacgtcgatt tcatgggtgg ctcaccccgt tgatcgatgg atgtaatcgc tatcccccgg
       61 cgcaacagat cgccatggtg ataccgtcat tatcgacggg gccggccggt gttaatgacc
      121 agcgacatta cctacagcgc cgtagtggcc gccacgggcg caaatgagtc gggtgccacg
      181 atttcgcaga ctgccggtca cggcgacttc cgtctaccta ctgactttcc ctctcgccag
      241 gtgatcattc gtttgccgag cgtgtcaatg cgaccgcgat agccgagccg ctaaatccct
      301 gcccaaacga ggggcggctt cacatctgag agattccttt tctaagtgat tgtcatgggg
      361 gaaccttccg tcggttctcc catgacagag ctccgacgag atctgtatat gtatgtatgt
      421 gattgacacc cggatagatg tgtaatcgta tgcagccacc tcgttccttg aagcgttgct
      481 caagttttca agataggtaa aagtttcact gccgtgtcct tgtcgccgta taaaagcagc
      541 gtcggcggtt gtgggccgtc aatgaacatg gtgccctgca gacttgaccg taaacagatt
      601 cctgcacgtt ctggttccca ggtcacctcg taaactattt cattctgtct tcgaatactt
      661 gacagttttc aagtgtttca gtaggatggc gaaaccttta atgctcatcc agtgatgtga
      721 gcaaggagcc aatcatgaac ttcggtccgg ccacgaccgg tgataatcga ccttctcaca
      781 atcgcacggt gaggaggttt ttttatgccc cgatttcaca agaacttgat cacatcgtta
      841 cttgatgatg acgactcccc tgaacaaacc ggtggaacgg aaagatgaaa agctgattat
      901 ggatgccggt gatatctccc gtgccatgat acgtatcgcg cacgagattt tggaacggaa
      961 caaaggggta aaggatctcg cactagtggg aatacggaca ggtggcgtgc atctcgccca
     1021 tcgactcgtg aagcgtatcc acggtatcga gggcgtccaa attccaatcg gtgaattgga
     1081 tatcacattg tatcgcgatg atctggcgtt gcgcaaaaat caaccgattc ttcgaaaaac
     1141 gtccgtgccg ttcgacatga cgaataaagt cgtcgtactc gtcgacgacg ttttgttcac
     1201 cggaaggacg atacgagcgg cgatggatgg gcttatggac ttgggacgtc cggaggaaat
     1261 ccaactggca gtgttgatcg atcgtggcca tcggcaactg ccgatcaaag ccaattacat
     1321 tgggaaaaat ctaccaacct ctcgggatga acaagtccaa gtgttgttgg acgaagatgg
     1381 ggaagatgac cgggtcgtca tcttgaaatc ctgaatcgga ggatcccatg agcctcaagc
     1441 gcaaatatct gctgagtctc gccccattat ctgtggatga gattaggctt gtcctggata
     1501 cggcagattc cttcaaggaa gtgacaggac gtgaaatcaa aaaagtgccg gctctccgag
     1561 gcaagaccgt cgtgaacctc ttttttgaac cgagcaccag aacccgcacg tcctttgagc
     1621 tggcggccaa gcgtctgagc gccgacgtga tcaacttttc cccctcctcc agtagcgtgg
     1681 tgaaagggga aacgctcctt gacacggctc ggaatattga agcgatgcag gccgacatca
     1741 tcgtgctgcg ccattcttct gcgggggccg cggaaacgct ggcgaacggc gtcaaatcgt
     1801 cggtcatcaa cgcaggcgat ggatggcatg agcacccgac gcaagcgttg ctcgacctgt
     1861 acacgattcg gcaacggggg atgaatttcc atggattgcg cgtggcgatc gtaggagatg
     1921 tgtcacatag ccgggtggcg cgctcgaaca tttatgcgct catcaagctt ggcgccgagg
     1981 tacgtctggt gggacctcct acgatgatgc cctgcggtgt ggaaaaactg ggagtgaaag
     2041 tgtactccga catggatgaa ggccttcgtg gagcgaacgt catcatgatg cttcggttgc
     2101 aactggagcg gcagggacgc gcgctgtttc cgacaattcg ggaatactcg cggctctatg
     2161 gcttaaccgc tgagcgagtg cggctggccg acgccggcgc catcgtcatg catccagggc
     2221 cgatcaatcg aggagtggaa atcgcgccgg atgttgccga tagtttgtcc tcggtgattc
     2281 tggatcaagt ggcgaacggt gtcgcagtcc gtatgggaat tttgtacctt atgtccggag
     2341 cgaattgagg atgttgcaaa actctccagc atcgttctcg catcgctcaa ggtctcaacg
     2401 taccaagcga gagagagttg ctctgccagc tcgggtgggc tgggtgctaa agattacgcc
     2461 tcgaccaaag acactgggcg ttcaccaact cgcgcccgtc cgcaaacacg aggctcctta
     2521 ttcgtcgcct tgcggacctc gctgcggcct tgctgggaga gcttaataag gagtgtgtcg
     2581 tgtcgatttt gattaaaggt ggtctcgtca ttgatccagg ccggtttgtc ggcattgccg
     2641 atgtgctgat cgacaagggc aagatagacg cggtgagacc gaacctttcc gccccggtcg
     2701 acggccggat cattcaagcc aatgggaagc tggtgatgcc ggggttcgtt gatttgcatg
     2761 tccattttcg tgagccgggg ttcgagtaca aggaaacgat ccagagcggc agtgctgcgg
     2821 ctgttgccgg aggatttacg acggtctgct gcatgacgaa tacgaacccg gtgaatgaca
     2881 atcaagccgt gacagaattc atattagaac gagcccggtt ggctggtctg gcaaatgtgc
     2941 tgccggtcgg cgccatcaca aaaggatcgg aagggaagga actggcggag atcggggatc
     3001 tgtgtcggtc cggttgcgtg gccatctccg acgatggcaa gcctgtgatg aacagcctgg
     3061 tgatgcggcg agcgatggaa tatgcattgg cgttcgatct gacggtcgtc gaccattgcg
     3121 aagacctgca tctggcggaa ggcggttgta tgaatgaggg attggtctcg accgagctgg
     3181 gattgcccgg tatcccggct gcggcggagg atgtgatggt cgcccgcaat ctctcactct
     3241 cggagttgac aggggctc
//
LOCUS       DATNDD010000387         6981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1780298, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000387 DATNDD010000000
VERSION     DATNDD010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780298"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VN647_09385"
     CDS             complement(<1..374)
                     /locus_tag="VN647_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97283.1"
                     /translation="MRNFNQKFRLRDADRHIRLLYTLFLLLMLAGFAFSFFWAHSMTS
                     LSPQGIADHYRGSDTTFGEPMSFRELAEITHFHLFTMPVVFMILIHVMYLTSASQILK
                     TVVTWAGFGGVILDLLSPWLIS"
     gene            complement(399..1067)
                     /locus_tag="VN647_09390"
     CDS             complement(399..1067)
                     /locus_tag="VN647_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWG97284.1"
                     /translation="MIIGLFSILAVLFVMPSPSRAERVWDSDLKRYLTDQELNLATVY
                     LQEEDGIKIMFPKSERVRKELITLNPEKKSLIEERIGWKFPEESFEVYIGESGTQIDG
                     YAVVQNTIGKHKPMTYMVGVDNEGSVSNVELLVFRESRGSEVRKKRFNVQYEGKTVFD
                     PVRIKRDITNITGATMSVRSMSAGVKRVLVLIDEFYLKPAGIGSDTMAAQKNKGFFSS
                     IFGN"
     gene            complement(1121..2620)
                     /locus_tag="VN647_09395"
     CDS             complement(1121..2620)
                     /locus_tag="VN647_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97285.1"
                     /translation="MKMRSILGALVMVSLSVMPVCAENLTPEEIKKMVDEAVEKRLQE
                     RERGGPPVEPREENASTEPAAEPGVGPIPEVARDLRAKERIPLSFASTGSGRLLYAKP
                     FLTFPKAIVGGYFDIQYRMHRNSVMETGDYNDIRGGQGTSSGFDSQRFVPFIYADITE
                     HVKFASELEIEHGIRETNDIEVSAEFAFVDYVIHESVNLRGGIILLPIGKFNLLHDSP
                     LNDLTDRPLVSTLIIPSTISETGAGMYGTFYPGRTGKLDYEVYVTTGPCGYTAAGAPK
                     VTEFDGTKNSRQRKCLSDDGLDINNGRTVVGRVAYSPMLGVEIGGSGYYGNQSPSSYN
                     PLSIFAVDWTLQKGPFELIGEAAWAYARNNAIAIPRNTIGFAPGARLTGIGTLNPLGA
                     PPERMQGFYIQGNYHFMPSFLTNLSPKRFGEGSTFTAVIRYDRVNLNLDNKAENAGEL
                     EQISFGLNYRPIQDTAFKISYQYMPKSFNPNDGQRIHDQALVISAATYF"
     gene            complement(2773..3243)
                     /locus_tag="VN647_09400"
     CDS             complement(2773..3243)
                     /locus_tag="VN647_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97286.1"
                     /translation="MAKFLRMGLLMVPLILIESLGGCALMSGMQERSALCSYDDAWDA
                     AVDTVKDRSTDRKDKEAGLIVTHWLEIPMPGRTYGIFGRNIADSRDRSRLTLEVKRRD
                     EVAKISFIEERQSWAFRGGARLFGWTPTDPSEEVMRDVRNRIDANLKEHGCTVS"
     gene            complement(3411..3590)
                     /locus_tag="VN647_09405"
     CDS             complement(3411..3590)
                     /locus_tag="VN647_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97287.1"
                     /translation="MTITKTPMLERDQTLETRCECGQLIAKVCGQGLELKCKRCKRIV
                     IIPFSSIEGWGNEPS"
     gene            3746..4339
                     /locus_tag="VN647_09410"
     CDS             3746..4339
                     /locus_tag="VN647_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97288.1"
                     /translation="MFRAGLLGVLVLSMSGCVTELPSLVDQDQVAGSLVVGRVITVLT
                     GERSRRYVPQMRFLELEDKDSQQRFQVSIESRDQRFALDLPAGNYRLNRAQISEGPFM
                     SMADLDMTFTVEAGVINHMGIWRFGVDSPRYGRNVIVSMIPEERAMVRTFLKEHYPAF
                     NPHSMVERLPQPSQVEARLYEVMPYPRVSRYFRRQWW"
     gene            4812..5837
                     /locus_tag="VN647_09415"
     CDS             4812..5837
                     /locus_tag="VN647_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWG97289.1"
                     /translation="MKTSFTHVDSMMMLCFAMILMVGCVGMSPTSQPVVSKRTQMHMG
                     TLVTITVVASDKDAGYRAIQTGFDEIKRLERLLSTWRPDSELSHVNAEAGRLPVQVGQ
                     ETLELVVRSIEMAQLTDGGFNIAVGPAMEAWSFTERQRIPSGEELQRLKPLVDWTTVQ
                     ISKEARTIFLPHKGMRVDIGGIGKGYAADRAVEEMKRAGAQGGVVALSGDIKTFGLLP
                     EGEGFLVGIKHPREDEALIAVIDLKDEAISTAGDYERFFVRDGIRYHHILDPQTLRPA
                     RGCQSVTVIAKEGAVADGLDTGIFVLGPTRGMELVERLPDVEAIIIDDKGTVTVSSGL
                     RDRLRAP"
     gene            5890..6249
                     /locus_tag="VN647_09420"
     CDS             5890..6249
                     /locus_tag="VN647_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97290.1"
                     /translation="MKTTLRGSLRLLLVALVALFACSEFETAYFQDKVDHVTQNVVAG
                     RYGMPHKEERLPAGGSVWTYFDRGSGTVGYTGHAESSKCKAYLLTFDQSGVLRDWKQQ
                     DCMSQSGPITEPSSDHN"
     gene            complement(6221..6868)
                     /locus_tag="VN647_09425"
     CDS             complement(6221..6868)
                     /locus_tag="VN647_09425"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWG97291.1"
                     /translation="MPLSSSHTAPTHEQPSPTTQASPDLLPQSPSLAAEPMDRSEHEP
                     LPHPVATGSEAPEPPAQVELPTQPYPISGQPQPPLQHQRVSSAEDEITQPPSIASVSA
                     VPSPADTAESRPTRAQYGWLTDLMANWIEDLNKRYPTLLRTEGIQGKVTLTAMLHDDG
                     LLSDVRVIKSSGNRALDQVALEDVRNGPPIKLSRPLERPHMSVRFSISYDLKTVQ"
BASE COUNT         1572 a         1755 c         1954 g         1700 t
ORIGIN      
        1 tagctgatca accatggcga caggagatcg agaattacgc cgccgaagcc ggcccaggtc
       61 acgacggttt tcagtatctg acttgcactg gtcagataca tcacatggat gagaatcatg
      121 aatacaacgg gcatcgtaaa aagatggaag tgagtaattt ctgccaattc ccgaaaggac
      181 atgggctccc cgaacgtcgt atcggaccct cgatagtgat cggcaatgcc ttgcggggag
      241 agactcgtca tgctatgcgc ccaaaagaaa gagaaggcaa agcccgccaa catcaagagc
      301 aagaacaggg tatagagcaa ccgaatatgt cgatcagcat cacgcagcct gaatttctga
      361 ttaaagtttc gcatgccggg acgctgggtc cgcttgaact aattcccgaa tatggacgaa
      421 aagaatcctt tatttttttg tgcagccatt gtgtcgctgc cgattccggc cggttttaag
      481 taaaactcgt cgatcagtac caatactcgt ttgactccgg cgctcatcga gcgaacggac
      541 atcgtggcgc cggtgatgtt ggtaatgtct cttttgatcc ggaccggatc gaaaacggtc
      601 ttgccctcat actggacgtt aaatcgcttc ttccgaacct cgctcccgcg cgattcgcgg
      661 aacacaagca gctcaacgtt cgatacggaa ccctcgttat cgacgccgac catgtaagtc
      721 atcggcttgt gcttgccgat cgtgttttgg acgaccgcat agccgtcaat ctgagttccg
      781 gattctccga tgtacacctc gaatgattcc tccgggaatt tccagccgat gcgctcttcg
      841 ataagagatt ttttctcagg gttgagtgta atgagttcct tccggacccg ttcggacttc
      901 ggaaacatga tcttaatgcc gtcttcctcc tgcagataga cggtggccag attcagctct
      961 tgatcggtga gataacgttt gaggtcgctg tcccacacac gttcggcgcg ggaaggagag
     1021 ggcatcacaa aaagaaccgc tagtatggaa aatagaccaa tgatcatgag gtcccctcaa
     1081 ttgcggggag gccggccgta acgaccggcc tcccgggtct ctaaaaatag gttgccgctg
     1141 aaataaccaa tgcctggtcg tgaatgcgtt gtccgtcgtt tgggttaaag gatttcggca
     1201 tgtactgata gctgatcttg aacgccgtgt cttggatcgg acggtagttc aacccaaacg
     1261 aaatttgctc gagctcgccc gcgttctctg ctttgttgtc caaattcaaa ttgactcggt
     1321 catagcggat gacggcggtg aaggttgaac cttcaccgaa acgctttggg gagaggttcg
     1381 tcaaaaaaga aggcatgaaa tgatagttgc cttggatgta gaatccctgc atccgctcag
     1441 gcggagcgcc gagcgggttg agtgtgccga tgccggtcag ccgagcaccc ggcgcgaatc
     1501 caattgtatt tcggggaatc gcgatagcgt tatttctcgc ataggcccag gcggcctcgc
     1561 cgatgagttc aaacgggccc ttttgaagag tccagtctac ggcaaagata ctcagcgggt
     1621 tgtagctgga aggggattga ttcccataat acccggaccc gccgatttcg acgcccagca
     1681 tcgggctgta cgcgacgcgt cctacgaccg ttcttccatt attgatatcg agaccgtcgt
     1741 cggacaagca tttccgttga cgggaatttt tcgtgccgtc gaactcagtg accttagggg
     1801 caccggcggc tgtatagccg caagggccgg tcgtgacgta gacttcataa tccagcttgc
     1861 cggttcgccc aggatagaac gtaccgtaca tgcccgcgcc tgtttccgat atggtgctcg
     1921 ggatgatcag tgtgctgacc aacggtcgat cggtcaagtc gttcaacggc gaatcgtgca
     1981 gaaggttaaa cttgccgatg gggagaagga tgatgcctcc tcgtaggttg acgctttcat
     2041 gtatcacgta gtcgacgaat gcgaactctg cgctgacttc tatatcattg gtctccctga
     2101 tcccatgttc gatctcgagc tcagacgcga acttcacatg ctcggtaatg tccgcataga
     2161 taaatggaac gaaacgctgc gagtcgaatc cgctcgaggt gccttggcca cctcgtatgt
     2221 cgttgtagtc gcccgtttcc atgacagagt tgcggtgcat gcggtattga atatcgaaat
     2281 agccgccgac aatcgccttg ggaaacgtca ggaacggttt cgcgtacagc aggcgaccgg
     2341 atccggttga ggcgaaggac agagggatcc gctctttggc gcgaagatcc ctggcgactt
     2401 ctggaatagg gccgacccca ggttcggcgg ctggttctgt tgatgcgttc tcttctcggg
     2461 gttctaccgg tggtcctccc cgctcacgtt cctgcaagcg tttttccacc gcttcatcga
     2521 ccattttttt gatttcctcc ggggtcaagt tttcggcaca aaccggcatc accgataggc
     2581 tgaccatgac aagagccccg aggattgacc gcatcttcat cggtatcctc cctcatgact
     2641 gagattgtag tttgactcgg gactcgatgg tctctggcct tcataagaat gacatgcact
     2701 ccatatataa aatggagata tgactatgcg ccacgcgctt ctattggtat caaaaatatc
     2761 ttcggagtag ggtcaactga cggtacatcc gtgctctttc agattggcat caatacgatt
     2821 tcgcacgtcg cgcatgactt cttccgaagg gtccgtcggc gtccagccga agagacgcgc
     2881 tcctccgcga aatgcccagc tctgccgttc ttcgatgaaa gagatctttg cgacctcgtc
     2941 ccgtcgcttc acttccagtg tgagacggga ccgatccctg ctgtccgcta tgtttcttcc
     3001 gaaaatgccg taggttcgtc ctggcattgg gatttcaagc cagtgcgtga cgatcaatcc
     3061 agcctcttta tcctttctgt cggtcgagcg atcctttacg gtatcgactg ccgcatccca
     3121 ggcatcatcg taactgcaca aggcagaccg ttcttgcatc ccgctcatca gggcgcagcc
     3181 gcccaggctc tcgatcaaaa taagtgggac cattagcaaa cccattcgaa ggaacttcgc
     3241 catatcacga actccccgcc ggggagagtc tcctttcatt ccggttaaaa gtaagtcgcc
     3301 ctctggaaga ggaagccatc tccgtcgatc ggactgttag cggtgcccaa atctccggcg
     3361 ggtatcaatc ctgctgcgct gttctgcgcg gtatcttttc gtacatggaa tcatgatggt
     3421 tcgttccccc aaccctctat cgatgaaaag gggatgatga cgatgcgttt gcaccgttta
     3481 cacttcagct ccagtccttg tccgcacacc ttggcgatca gttgcccgca ctcgcagcgc
     3541 gtttcaagag tctgatcacg ttccagcatg ggcgtcttcg taatggtcat gatacgtggc
     3601 cgccaatggc attcaaatgg attaaaattg agaacgatta ttaatatctc aatcagtctt
     3661 ccgttgtcaa gcgattagtt ttcgccgcgc ggtatgccat tatcagcacc gctcgtcata
     3721 taattttttt atgaagaaga tccagatgtt tcgagcaggt ctcctgggag tgctcgttct
     3781 gagtatgtcg ggttgtgtga cggaattgcc ttcactggtc gatcaagatc aggtcgcagg
     3841 tagcctggtc gtagggagag tcataacggt tctgacaggt gaaaggtccc gacggtatgt
     3901 accacagatg cggtttctgg agttggagga caaggattcc caacaacgct tccaggtgag
     3961 catcgagtct cgggatcaac gttttgctct cgatttaccg gccggaaatt atcgactcaa
     4021 ccgagcgcaa atcagcgagg ggcctttcat gtccatggcc gatttggaca tgacctttac
     4081 cgtagaagca ggtgtcatca atcatatggg aatatggcgg tttggagtcg attcaccgcg
     4141 ctatggacgg aacgtcattg tttcaatgat tcccgaagaa cgagccatgg tacgtacctt
     4201 tctgaaggaa cactatccag ccttcaaccc gcactccatg gtggaaaggc tccctcagcc
     4261 atcacaggtg gaagcacgcc tctatgaagt catgccttac cctcgggttt ctagatactt
     4321 ccgtcgacag tggtggtgaa gatcattaag gaacttcgtc atgtcacgag ctccccttcg
     4381 gggaacggcg gggaagtctc ttttcattac ggttagaagt aagtcgccgt caatctataa
     4441 tcaagatgtg actgcaatgt ctcctttcgc aatgattctg atgttgcggt tccccagctt
     4501 tcgattgaag agaagaaatg acggccatgc gcttacaccg tttgcgttta agctccagat
     4561 cgcgcgagca taccgtaaca atcaattagt ttatataggg taagtgtgaa cgacccttaa
     4621 cattcaagta ggtgttaatc tgtcaagcga taagtttcgg cagcgtgagg ctatctgtgc
     4681 gttcaatgtg cgttgctcta taattcccat agaaatgaag ctcgagatag aagcgcggcc
     4741 ctatgagatc tcgccctatc ctcggtatct cacaatgttg tgccgaccct tgtgttgagg
     4801 aagcgaccgc aatgaagaca tctttcaccc atgtcgattc gatgatgatg ctctgcttcg
     4861 ccatgatttt gatggtcggc tgtgtaggaa tgtcaccgac ctctcaacct gttgtctcga
     4921 aacgtactca gatgcacatg gggacgctgg tgacgatcac cgttgtggcc tccgacaagg
     4981 acgcgggcta tcgggctatt cagactggat tcgatgaaat caagcgactt gaacggttgt
     5041 taagcacctg gcgtcccgac agtgaactgt cacatgtgaa tgcagaagcc ggccggctgc
     5101 cggtgcaggt gggtcaagag acactggagt tggtggtccg atccattgaa atggcgcaac
     5161 tgacggatgg cggctttaat atcgccgtgg gtcctgcaat ggaggcctgg agttttacag
     5221 aacgacaacg tattccgagc ggcgaggagc tacagcgact gaagccgctc gtcgattgga
     5281 caaccgtgca aatcagtaaa gaggcacgaa ccatttttct tccacacaag ggaatgcgtg
     5341 tcgatatcgg ggggattgga aaagggtatg cggcagatcg ggcggtagag gagatgaaac
     5401 gggcaggagc tcagggtggg gtggttgcct tatcgggaga tattaaaacc ttcggcctcc
     5461 ttcccgaggg tgaagggttt ctcgtaggga tcaagcatcc acgtgaagat gaggcgctga
     5521 ttgccgtcat cgatttaaag gacgaggcaa tttcaacggc aggtgattac gaacggtttt
     5581 tcgtacggga tggaattcga tatcatcaca ttttggatcc tcagaccctg cggccggccc
     5641 gtggttgtca aagtgtcacg gtcatcgcca aggagggggc ggtggctgat gggttggata
     5701 caggaatctt tgtgctgggt ccaacgcgcg gtatggagtt agtcgaacgc ttaccggatg
     5761 ttgaggcgat catcattgac gacaaaggga cggtgaccgt ttcgtcagga ctccgtgatc
     5821 ggctgcgcgc gccatgagaa gtgcctatat gtgctgttat gatgatcaac acaggatgag
     5881 gacgacacaa tgaaaacaac tttacgagga tcgctgcgtc tgttgcttgt tgcgttggta
     5941 gccctgttcg cctgcagcga gtttgaaacg gcatatttcc aagacaaggt ggaccacgtt
     6001 acacagaacg tggtggccgg ccggtacggt atgccgcaca aggaggagag attgccggcg
     6061 gggggatcgg tctggaccta ttttgatcga ggcagcggca cggtcgggta tacaggtcat
     6121 gcggagtcga gcaagtgcaa agcgtatctc ctgacgttcg atcaatcggg cgtattgcgc
     6181 gattggaaac aacaagactg catgtctcaa tccgggccga ttactgaacc gtcttcagat
     6241 cataactgat ggaaaacctc accgacatgt gaggccgttc taatgggcgg gaaagtttga
     6301 tgggaggacc gtttcggacg tcttccaagg cgacttgatc gagcgcccgg ttgcctgagc
     6361 tcttgatgac gcgcacatcg cttaggagtc cgtcgtcgtg aagcatcgca gtcagcgtca
     6421 ccttcccctg aatgccttcc gttcgcaaca gggttggata acgtttattc agatcctcga
     6481 tccagttggc cataaggtcg gtgagccatc catactgggc acgagtcggc ctggactccg
     6541 ccgtatctgc agggctaggg acagcggata ctgatgcgat agaaggcggc tgggttattt
     6601 catcttcagc cgacgaaaca cgctgatgct gtaggggcgg ttgaggttga ccggaaattg
     6661 ggtaaggctg agtcggcagc tccacctgtg ccggcggttc aggagcttcc gatcctgtcg
     6721 ctaccgggtg aggtaacggt tcatgctcgg atcggtccat aggctctgcg gcaagcgagg
     6781 gtgactgcgg gagcaggtct ggggatgctt gagtggtcgg ggatggctgt tcatgagtcg
     6841 gagcggtgtg cgatgaagac aacggcaccg gtgcttcgat tcttggccga cccgatcttg
     6901 ttcttgaatc ggcggtcgcc gtggatcgtt ccgatgcagg agtcgtggag ggccttgacc
     6961 ttacagccgc gatagaatcg a
//
LOCUS       DATNDD010000388         8099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1878350, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000388 DATNDD010000000
VERSION     DATNDD010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878350"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VN647_09430"
     CDS             <1..1252
                     /locus_tag="VN647_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWG97292.1"
                     /translation="AELKAKPATDESKKRGSELVVEKKCVECHGMQGRGDGNAFNLKD
                     DWGFSIQPADWHKCWNFRGSRQDPYNVANIFRTFSTGVNGTPMPSFADNTTVDERWDI
                     ANFVNSLCERDALGNPLPIDPLTDKPKINFVIPSDLFEGEIPTDIEHAAWQKAPKRYV
                     AMGGQITHKPRNFVNRIDDIWVSSLYNDKSIVYLIEWDDRTKSVAEGKLPWVPTQVNI
                     DVKEQDPKTGEEGSIAAHQNNYTVYNDAIAIETAVKWKELPAPIKPRYLFGTNDQFPV
                     DIVKWEADGSLRAFKGTGWDKDFEERDNYEESMKLLKGEWKDGRWYVMIQRPLGNKKD
                     QDYDEDTFFEMGQYIPTVFFAWDGHNGDAGRKMAVSAFYYTFMNPPIPRETYIYPAVI
                     AVGVVLLEGWVLTRRANKKKGKML"
     gene            1343..1960
                     /locus_tag="VN647_09435"
     CDS             1343..1960
                     /locus_tag="VN647_09435"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWG97293.1"
                     /translation="MISDVTGVLLAGGKSRRMGEDKRFILVGQRTLFERSCAVLCGLF
                     EQVCVVIAQDSPSLQTEVPVVRDLIPDCGSLGGLYTGLRLAKTQHIFLAACDMPFLNS
                     DVIRYMVHLKDQADIVISRWVTRLQPTHAVYGRNCLPVIEEMMKLHNRKIHGMIGHPA
                     LRVSFITEREIQQIDHDGRSMFNINTPSDLEHARSVHDADTDLGS"
     gene            1976..2974
                     /locus_tag="VN647_09440"
     CDS             1976..2974
                     /locus_tag="VN647_09440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWG97294.1"
                     /translation="MKRTILILHPGALGDVLLAVPAIRSLHGRFPQHEIVLIASAAVS
                     RLLSECGVIDDWISLEGQVCLGLFSETLSISEKLQSRVDRCDLAVAWTEDKDGVLRSL
                     FQEAGVGLVQIQSPFAMGLRSRHQGNRFLETLGKIAGDISPEETIHVSPHLLEWGRDY
                     LETLGISPDQSLVLVHPGSGSIHKCLEPGRVALLIEQLWRGGMCPLVLEGPADRDAVG
                     HALQFVSKPPFVLRNLDLSRLASVLAQVTFYIGHDSGVTHLSALLGVRTIAFFGPTDP
                     QRWAPQGSHITILRGSSCRCESWETVRKCAEKPCLQVPIEEILTALRGGKEQHKPS"
     gene            3066..4790
                     /gene="argS"
                     /locus_tag="VN647_09445"
     CDS             3066..4790
                     /gene="argS"
                     /locus_tag="VN647_09445"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWG97295.1"
                     /translation="MSQGLVQERVTTALLGALNDARQKGQLKTTAWPTLSLDAPKRPE
                     WGDLASTVAMSLASSEHKAPHDIAQIIVENLPQREQLFDRVEIVRPGFLNLTVKPALW
                     QEVLREIEFQGARYGRAAVGTGRRVLVEYVSANPTGPLHVGHGRGAAVGDAVVRLLNA
                     IGYEAVGEYYINDAGRQMKLLGASVYARYQELSERTVEFPEDGYHGSYITAVAQQIKE
                     QLDRVADGLTPADLEARCRTLAYQILLELIRDDLQSFGIEIQSWFSEASLLESKAIER
                     ALAELKSRDLLFQQEGAWWFRASMFGDEKDRVVKKQDAEYTYLASDIAYHHDKLRRGY
                     DLLIDVFGADHHGYIPRMQAVMQAYGHPKDRLHVVLVQLVKLLRDGVEVKMSKRTGEF
                     ITMREVIDEVGADAAKFYFLMRDSKTHLEFDLELAKQRSADNPVYYVQYAHARICSLW
                     RVASERGVTRPLALGTDLTVLTDPDELGLIKKLSAYPEIIQASALAFEPHRVTYYLQQ
                     LAALLHTFYNKHRVLPPAADQEHDESASAEILTPKRTAARLVLMGAVQQVLRNGLEVL
                     GISAPEQM"
     gene            4813..5988
                     /gene="tgt"
                     /locus_tag="VN647_09450"
     CDS             4813..5988
                     /gene="tgt"
                     /locus_tag="VN647_09450"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWG97296.1"
                     /translation="MMQYHVQHFDRHSKGRIGLLTTGHAAVDTPAFMPVGSLGPVKGL
                     EPEDLQSLGFRLILNNAYHLYLRPGHKLVADMGGLHAFTGWPGAILTDSGGFQIFSLA
                     KLCEITDAGVIFQSHIDGSTHFITPEKTIEIEEALGADIVMVLDQCAALPAGREIIEE
                     SVRRTKLWAERCQASRRRTDQALFGIVQGGLETDLRVASARELVKLGFEGYAIGGLSV
                     GEDKSDMYAMLDVTVPELPEKKPRYLMGVGHPEDLLEGVARGIDLFDCVVPSRHGRTG
                     SLFTATGRVVIKQAQYAEDERPIDPACTCPVCRRYSRAYLHHLFMIKEMLGARLNTIH
                     NLWYFSDFMRRIREALVEGTFPEFREAFYRDYDRQAVMAGSAGCEKSCGELHGNGHA"
     gene            6006..6362
                     /gene="yajC"
                     /locus_tag="VN647_09455"
     CDS             6006..6362
                     /gene="yajC"
                     /locus_tag="VN647_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWG97297.1"
                     /translation="MLMESIAWAEGTGGGGSPASGGAGGILSLIPFLLIFVIFYFLLI
                     RPQQKKQKQQQALLDAVKKGDKVVTTSGIWGTITNMGKETVTLQIADNTKIKMQRENI
                     ARVRSEEEDKDKDKDT"
     gene            6392..8029
                     /gene="secD"
                     /locus_tag="VN647_09460"
     CDS             6392..8029
                     /gene="secD"
                     /locus_tag="VN647_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWG97298.1"
                     /translation="MKKVSGRLWLLTLVALVSVVAVLPSYQPVYQALPGWVKGALPNK
                     GITLGLDLQGGIHMVLEVDEDRAVEIAVDRSVNALQDLLTEKKLAVDSVKRIGHDQIT
                     IQLQNADAKVSVQKLIDNFPIFVEKEPAGATSVVVWELREQETKRMKDSAINQALETI
                     RNRIDQFGVAEPIVLRQGLKQIVVQLPGVKDPKRAKDLIKETALLEFKMLDEDIHLDL
                     PARVSKDKETEVIQQFAGKMPEGDQILFERMIDKDTGVEFRIPYVVKKRVMLTGDVLS
                     DARVAIGQFNDSYVSITFDSKGGQEFERITGENVKKRMAVVLDNTIYSAPVIQERISG
                     GRAQITGTFTTQEANDLAIVLRAGALPAPLKIVQDLTVGPSLGQDSIDKGVRATLIAG
                     AMVVVFMIVYYRLSGLIADFALVLNLVCLMGALSALTATLTLPGIAGIVLTIGMGVDS
                     NVLIFERIREELRGGKAPRSAVDAGYDKALLTIIDSHVTTLITGVALFLFGTGPIKGF
                     AVTLCLGIAINLFTALVGTKVIFDLLYHRQKVEALSI"
BASE COUNT         1886 a         1942 c         2376 g         1895 t
ORIGIN      
        1 ggctgagctc aaggcaaaac ccgcaaccga tgaaagcaag aagcgcggtt ccgaactcgt
       61 cgtcgagaag aagtgcgtcg aatgccatgg gatgcaaggg cgtggagatg ggaacgcctt
      121 taatttgaag gacgattggg gtttctcgat tcagcccgcc gattggcaca agtgctggaa
      181 cttccgtggg agccgtcagg atccatacaa tgtggccaac attttccgaa ctttctcgac
      241 gggtgtcaat ggcaccccga tgccatcgtt tgcggataac acgacggtgg atgaacggtg
      301 ggacatagct aatttcgtta attccctgtg tgagagggat gccttaggca atccgctccc
      361 gatcgatcca ttgacggata aaccgaaaat caacttcgtg atcccatcgg atctctttga
      421 gggtgaaatt ccgaccgata ttgaacatgc ggcttggcaa aaagctccaa agcgatatgt
      481 ggctatgggt ggacaaatta ctcataagcc caggaacttc gttaaccgga ttgatgatat
      541 atgggtgagc tcgctctata atgataaatc aattgtctat ctcatcgagt gggatgacag
      601 aaccaagagt gtggcggaag gaaagcttcc ctgggtccca acccaggtca atatcgatgt
      661 taaagaacag gatccgaaga caggcgaaga aggatcgatc gcagcgcacc aaaacaacta
      721 cacggtgtat aacgatgcga ttgccattga gacggcagtc aagtggaaag agctacctgc
      781 gccgatcaag ccccgttact tgtttggtac caacgaccaa ttcccggttg atatcgtcaa
      841 gtgggaagca gacggatccc tccgcgcgtt taaaggcacg ggttgggata aggattttga
      901 agaacgtgat aactacgaag aaagcatgaa actcttgaag ggcgagtgga aggacggtcg
      961 ctggtatgtg atgattcagc gtcccttggg gaataaaaag gaccaagatt acgacgagga
     1021 tacattcttc gagatggggc aatacattcc aacggtgttc ttcgcctggg atggccacaa
     1081 tggtgatgct gggcgaaaga tggccgtctc ggcgttctat tacacattca tgaacccgcc
     1141 gatcccccga gagacatata tctatccggc agtcattgcc gttggagttg tgttgctgga
     1201 aggatgggtt ctgactcgtc gtgccaacaa gaaaaagggt aagatgctgt agcattcctt
     1261 agatacacgc gttagcgtag taagaagatc gagggggtgg ggcgacccac cccctctttt
     1321 ttttcaaaca ggtgacgtgc tcatgatcag cgatgtgacg ggcgtattgc tggccggggg
     1381 aaagagtaga aggatgggag aggataagcg atttatcctc gtcggtcagc ggaccctctt
     1441 tgagagaagc tgcgctgttc tctgcggatt gtttgagcag gtttgcgttg tcatcgctca
     1501 agacagcccg tcgctgcaaa cggaagtacc ggtggttcgc gatcttattc cagactgcgg
     1561 gagccttgga ggattataca cgggccttcg actggccaag actcaacata tcttcctcgc
     1621 tgcctgtgat atgccgtttt taaactctga tgtcattcgg tatatggttc acctgaaaga
     1681 tcaggctgat attgttatca gtcgatgggt gactcgtctg cagccaaccc atgcggtgta
     1741 tggccgaaac tgccttccag ttattgagga aatgatgaaa cttcataata ggaagattca
     1801 cggcatgata ggtcaccccg ctcttcgcgt gagtttcatt actgaaaggg agatccagca
     1861 aatcgaccac gatgggcgtt ccatgttcaa catcaatacg ccatccgatc tagaacatgc
     1921 tcgatcagtg cacgatgccg ataccgatct cggatcatag tgttcgagaa gagatgtgaa
     1981 acggaccatc cttattcttc atcccggtgc acttggggat gtcctgctgg ctgtcccggc
     2041 gataaggagt ctgcacggga ggtttccgca acatgagatc gtgctcatag cttctgcagc
     2101 cgtaagtcga ctcctatcgg agtgcggagt gattgacgat tggatttcat tggaagggca
     2161 agtatgtctc ggattgtttt cggaaacttt atccatctcc gagaaattgc agtcaagggt
     2221 ggaccggtgt gatctggctg tggcttggac ggaggacaaa gatggcgtcc ttcggtctct
     2281 atttcaggaa gcgggcgtag ggctggttca gattcagtcg ccatttgcca tggggttacg
     2341 ttcgcggcat cagggcaatc gttttcttga gacgctgggt aaaatcgccg gagatatttc
     2401 tccagaggaa acaatacacg tttctccgca tctcttggag tggggtagag attatcttga
     2461 gactttgggg atttcccctg atcaatcgct ggtccttgtg catccgggca gtggaagcat
     2521 tcacaagtgc cttgaacccg gaagagtggc cttgctgatt gaacaattgt ggcgaggagg
     2581 aatgtgtcca ctagtcctgg aaggaccggc agatcgggat gctgtaggcc atgcattgca
     2641 gtttgtgagt aagccacctt ttgttctcag aaacctcgat ctttcccggc ttgcgagcgt
     2701 gcttgcgcag gttacgttct atatcggtca tgattctggt gtgacgcatc tgtccgcttt
     2761 gttgggtgta cggacgattg ccttcttcgg tcctacagac ccacagcggt gggccccaca
     2821 gggtagtcat ataacgatcc tgcgtgggtc atcctgtcgc tgtgagtcgt gggaaacagt
     2881 gagaaagtgt gcggagaaac cttgtcttca agtgccgata gaagagattc tgacagcatt
     2941 gagagggggg aaggagcagc acaaaccctc gtaattccac atgaaccgcc ttgtctctac
     3001 ctatctcatg tgatagagtg gcgcgttaat ttcctttctt tggtaagggc ctagagagaa
     3061 ttgttgtgtc tcaaggactc gtgcaagagc gggtaacaac ggccctgctt ggcgccctca
     3121 atgacgcgag gcaaaaaggg cagttgaaga cgacggcttg gcccacactt agccttgacg
     3181 cgcccaaacg gccggagtgg ggcgatctcg cttcgacggt ggcgatgtct ttggcctcct
     3241 ctgaacacaa ggcaccccac gatatcgccc aaatcatcgt agaaaatctt cctcagagag
     3301 agcagctgtt cgatcgtgtg gaaatcgttc gtcccggttt cttgaatctt acggtgaaac
     3361 cggccctctg gcaggaggtt ctccgtgaaa tcgagttcca aggggctcgc tatggccggg
     3421 cagctgtcgg aaccggtcgt cgagtgctgg ttgagtatgt gagcgccaat ccgaccggtc
     3481 cattgcatgt cggccatgga cggggggcgg cggtcgggga cgcagttgtt cggctactga
     3541 atgcgatcgg atacgaggcc gtgggcgagt actatatcaa cgacgcggga cgacagatga
     3601 aattgttggg cgcatctgtc tatgctcgat atcaagagtt gtcggagcgg accgtcgagt
     3661 ttcctgagga tggttatcat ggctcataca tcaccgctgt ggcacagcag atcaaggagc
     3721 aacttgaccg cgtcgctgat ggactgactc ccgccgatct tgaggctcgc tgccgaactc
     3781 ttgcttatca gatcctgtta gaactcattc gtgatgacct tcagtccttt ggtatcgaaa
     3841 ttcaatcctg gttcagcgaa gcttcgcttc tagagtccaa agccatcgaa cgagctctgg
     3901 ctgaattaaa atcccgcgac ctcctgttcc aacaggaagg ggcatggtgg tttcgagcat
     3961 ccatgttcgg cgacgaaaaa gaccgtgtcg tcaagaagca ggacgctgag tatacatact
     4021 tagcgtccga tatcgcctat caccatgaca agctgaggcg tggctacgat ctgctgattg
     4081 atgtctttgg cgccgaccac catgggtata tcccgcgtat gcaagccgtc atgcaggcct
     4141 atggacatcc aaaagatcgt ctccatgtcg tgctggttca gttggtgaag ctgctacggg
     4201 atggagtcga agtgaagatg tctaagcgga ccggggaatt cattacgatg cgggaagtca
     4261 tcgatgaggt cggagccgac gctgcgaaat tctatttttt aatgcgggac tccaaaactc
     4321 atctcgaatt cgatctggag ttggcgaagc aacggtccgc cgacaatccg gtgtactacg
     4381 ttcaatatgc ccatgcgagg atctgcagcc tctggcgtgt ggcatccgaa agaggagtga
     4441 cccgaccgct cgcattgggg acggatctga ccgttttgac cgaccccgat gaattggggc
     4501 tgatcaaaaa actttccgcc taccctgaaa ttatccaagc cagcgcgttg gcctttgagc
     4561 cgcatcgagt gacgtattat ctccagcaat tggcggcgct tctccatacg ttttataaca
     4621 aacaccgtgt gttgcctcct gcggccgatc aggagcatga cgagtcggcg tccgccgaaa
     4681 tcctcacgcc gaaacgaact gcggcgcgtc tggtcttgat gggggctgtc cagcaggttc
     4741 ttagaaatgg acttgaggtg ctcggcattt cagcgcctga gcaaatgtag cgggtcagac
     4801 ctcccataat agatgatgca ataccacgtc caacatttcg accggcactc caaaggccgc
     4861 atcggcctgc tcacgaccgg tcatgcggcg gtcgacacac cggccttcat gccggtaggc
     4921 tcgctgggac cagtcaaggg tctcgaaccg gaggacctgc agagtctggg gtttcgactc
     4981 atcctcaata atgcctatca tttgtatttg cgccctggtc acaagctcgt tgccgatatg
     5041 ggtgggcttc atgccttcac cggatggccg ggagcgatcc tcaccgacag cggcgggttt
     5101 cagatcttca gtttagcgaa gttgtgcgaa atcaccgatg caggcgtcat atttcaatcg
     5161 cacattgatg ggtcgacgca cttcatcacg cccgaaaaga cgatagaaat cgaagaagca
     5221 ttgggagccg atatcgtcat ggtgctggat caatgcgcgg cgttgcctgc cggtcgagaa
     5281 atcatcgagg aaagtgtgcg tcgaaccaag ctttgggccg agcgttgtca ggccagcagg
     5341 cgaagaacgg atcaggcctt gtttggcatc gtacagggcg gtctggaaac ggacctgcgc
     5401 gtagcctcgg cgagggaatt ggtgaaattg gggttcgagg gctatgcgat cggcgggctg
     5461 tcggtcgggg aggataagtc cgacatgtat gcgatgctcg acgtgacggt tccagagttg
     5521 ccggagaaaa agcctcggta tctcatgggg gtcggacatc cggaagatct gcttgaaggg
     5581 gtggcccgcg gcatcgatct cttcgattgc gtcgttccct cgcgccatgg cagaacaggc
     5641 tcgctgttta cggcaacagg ccgggtcgtt atcaagcaag ctcagtatgc tgaggatgaa
     5701 cgaccgatcg atcctgcttg cacgtgtccg gtctgccgtc gatactcacg ggcctatttg
     5761 caccacttgt ttatgatcaa agaaatgctg ggagcacggc tcaacacgat tcataactta
     5821 tggtactttt ccgacttcat gcgccgaata cgagaagcct tggtagaagg gacctttcct
     5881 gagtttcggg aagcgtttta tcgcgattac gatcgacagg cggtgatggc cggttcagcg
     5941 gggtgcgaaa aatcgtgcgg agaattgcat ggaaacggtc atgcatagaa aaaggggatt
     6001 gttcgatgtt gatggaatcg attgcatggg ctgaagggac gggcggaggt gggtcacccg
     6061 ccagcggagg ggcgggcgga atcctatcct tgatcccgtt cctcttgatc tttgtcattt
     6121 tttattttct tctgatccgg cctcaacaga agaaacaaaa gcaacagcag gcgttgttgg
     6181 acgcggtgaa gaagggcgac aaggtggtta cgacgtcggg gatctggggc accatcacca
     6241 acatggggaa agagacggtg acgctgcaga ttgccgataa taccaagatc aaaatgcagc
     6301 gcgagaatat tgcgcgagtg cgcagtgaag aagaagacaa agataaggac aaagacacgt
     6361 agttcaaagg gaataagggg tcacggacga catgaaaaag gtaagtggac ggctatggtt
     6421 actcacgctg gttgccttgg tatcggtagt ggccgttctc ccgtcatatc agccggtgta
     6481 tcaagccttg cccggctggg taaagggagc gcttccgaac aagggaatta cgttggggtt
     6541 ggatttgcag ggcggcatcc atatggtcct ggaggtagac gaggatcgtg ccgtggagat
     6601 cgcggttgac cgttccgtca atgcgctaca ggatctcttg accgaaaaga agctcgcggt
     6661 cgactcggtc aagcgaatcg ggcacgatca aatcacgatt cagctccaga atgccgacgc
     6721 gaaggtctcc gttcaaaaac tgatcgataa tttccctatt tttgtcgaga aggagccggc
     6781 gggggcgacg agtgtcgtgg tgtgggagct tcgtgagcaa gaaaccaagc gaatgaagga
     6841 ttccgccatc aatcaggcgt tggaaaccat ccgaaaccgg atcgatcagt tcggtgtcgc
     6901 ggagccgatc gtcctgcgac aagggttgaa gcaaatcgtc gtacagctgc ctggcgtcaa
     6961 ggatccgaaa cgcgccaaag atctgatcaa ggagacggcc ctgctcgaat tcaaaatgtt
     7021 ggatgaagat atccacctcg atctgcccgc gcgtgtttcg aaagacaaag aaaccgaggt
     7081 gatccagcag tttgccggga aaatgccgga gggcgaccag attttattcg agcgcatgat
     7141 cgacaaagac accggtgtcg agtttcgcat tccctatgtc gttaaaaaac gggtcatgct
     7201 gaccggtgat gtgctgagcg atgcgcgtgt tgccatcggt caattcaatg attcatatgt
     7261 ctccatcacg tttgactcca aaggagggca ggagttcgaa cggattacgg gcgagaacgt
     7321 caagaaacgt atggcggtcg ttctcgataa caccatctat tcggctccgg tcattcaaga
     7381 acgaatttcg ggaggacgtg cgcagattac cgggactttc actacgcagg aagccaatga
     7441 tttggcgatc gttctgcggg ccggtgctct ccctgcgcct ctgaagatcg tgcaggatct
     7501 taccgtcggt ccgtccctcg gccaagattc tatcgataaa ggggtcaggg ctactctcat
     7561 cgccggggcg atggtggtgg tcttcatgat cgtgtattac cgcctgtccg ggctgatcgc
     7621 ggactttgcc ttggtgctga atctggtgtg tttaatgggt gccctgtccg ccttgaccgc
     7681 tacgttgacg ctccccggca tcgccggtat cgtgctgacg atcggaatgg gcgtcgattc
     7741 gaacgtcttg atttttgagc gcattcgcga agagttgcgt ggtggaaaag cgccgcgatc
     7801 cgccgtcgat gcgggttacg acaaggcgtt attgacgatt atcgattcac acgtgacgac
     7861 gttgatcacc ggggtcgccc tgtttctctt tgggaccggg cctatcaagg ggtttgccgt
     7921 gacgctgtgc ctgggcattg ccatcaacct cttcacggca ttggtcggga ccaaggtgat
     7981 ttttgatttg ttgtatcatc gacaaaaagt cgaggcattg agcatctaac catgaagccg
     8041 tcagcgatca gccttcaggg ggagactcag ggttatcaaa agggagcgcg catgttaga
//
LOCUS       DATNDD010000389         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1031594, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000389 DATNDD010000000
VERSION     DATNDD010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031594"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VN647_09465"
     CDS             <1..822
                     /locus_tag="VN647_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWG97299.1"
                     /translation="VVSVFDHGFLYGDGVYETIRSYGPRIFMRDEHLSRLFRSAEEIG
                     LTIPIPMKNWEDILHEAMIRNDVGTDLQDAYLRITVSRGAGDIGLDPALCSSPTVVVM
                     AKLLVPAASRLYEGGVNVIVASTKRNLPYALSPQIKTTNFLNNIQAKREAIAAGAFDS
                     ILLNWEQHLTECTISNLFFVMDGTLRTPALECGLLDGITRNIVIRLAGELNIHIEEGR
                     YTVDQLYRADECFLTNTSMEIMPVTSVDCRPVGDGKPGPLTRKLRAQFVAVRGRF"
     gene            940..1641
                     /locus_tag="VN647_09470"
     CDS             940..1641
                     /locus_tag="VN647_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWG97300.1"
                     /translation="MPKQNKLHRSSIRRTGMAALVALSGSVLLTVPSDSQAEIYQYVD
                     AKGTISLTNVPSDARYRRIDLHPNRLHPVLSEQELEPVISRFSRQHQLHPALIRAIIK
                     AESDFDPRAVSRAGAIGLMQLMPQTAVKLEVRDLYDPEDNIGGGTKYLRQLLDRFRGN
                     LPLALAAYNAGERVVDRYRTLPPIDETRQYVRKVLRYYRLFLARDLGVTGHIIPSSEY
                     AVPRPASVSSNQPGR"
     gene            complement(1574..>2316)
                     /locus_tag="VN647_09475"
     CDS             complement(1574..>2316)
                     /locus_tag="VN647_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWG97301.1"
                     /translation="LDVTHLGSNFVKRRFPTIYATCLRHDIDITEEWIPVSPSAHYMM
                     GGVATDIDGATTLPGLFAAGEVACSGVHGANRLASNSLLEGLVFGMRAGVAAVAWISR
                     RSMPDLAHHVERLRRGRLERLEDAEKVRSSLRRTMWGQVGLVRSRESLVRATAQLARW
                     ERMVSRSFAGRADLEVKNMVQVAHCVAEAALWRENSVGAHYRSDFPGSKRPGWKQHSQ
                     VCLGIRTTGQADLKTPKRVVARHTPKTG"
BASE COUNT          528 a          722 c          579 g          487 t
ORIGIN      
        1 gtggtctcgg tcttcgacca tggatttctc tacggcgatg gggtctatga aacgattcgc
       61 tcgtacggcc cccgcatctt catgcgagac gaacatttat cacggctgtt ccgttccgcg
      121 gaagaaatcg gcctgacgat cccgattccg atgaaaaact gggaagacat tctgcatgag
      181 gcgatgatac gcaacgacgt cgggactgat ctgcaagacg cctatctgag gatcacggtt
      241 tcccgaggag ccggagatat tggactggac ccggcactct gttcgtcgcc gaccgtcgtc
      301 gtgatggcca aacttctcgt gcctgcggcg tcccgtcttt atgaaggagg cgtcaacgtg
      361 atcgtggctt ccacgaagcg gaatttaccg tacgccttgt ccccacagat caaaaccacg
      421 aacttcctga acaatatcca agccaaacgc gaagcgatcg cagcaggcgc attcgacagt
      481 atcctcctca attgggaaca gcatctgacc gaatgcacca tcagcaatct gttctttgtg
      541 atggacggca cgctgcgaac gcctgccctg gaatgcggct tactcgacgg catcaccaga
      601 aacatcgtga ttcggttggc cggggaactc aacatacaca tcgaagaagg tcgttatacc
      661 gtcgaccagt tgtaccgggc cgacgaatgc tttctcacga acaccagcat ggaaatcatg
      721 ccggtgacct ccgtcgattg cagacctgtc ggcgatggga aaccgggtcc gcttacccgg
      781 aagctaagag cgcaattcgt agcggtgcga ggccgatttt agtacaggcc tcccacctgc
      841 aagacctcat ctttccattt tcctccagct tctactgatc tcccaagcac aagatctgca
      901 tttccttgac agacagtgac agactgttat cgtttgacca tgccgaaaca gaacaaactg
      961 catcgttcct ctatcaggcg gaccggcatg gccgccctcg tcgcattgag cggcagcgtc
     1021 ctactgactg ttccaagcga tagtcaggca gagatttacc aatatgtcga tgccaaaggg
     1081 acgatatcgc tcaccaacgt tccctctgac gctcggtacc gccggatcga tctccacccg
     1141 aatcggctgc atcccgttct ctcggagcag gagttggaac cggtgatcag tcgattctca
     1201 cggcaacatc aactccaccc tgcgcttatc cgcgccatca tcaaggccga atcggatttc
     1261 gaccctcgcg cggtatcacg agcgggagcc atcggattga tgcaattgat gccgcaaacg
     1321 gcagtgaaac tggaggttcg tgacctctac gatcccgagg ataatatcgg aggaggaacg
     1381 aagtacttac gccagttgct cgaccgattc cgcggcaacc ttccgttggc gctcgctgcc
     1441 tataacgccg gggagcgtgt tgtcgatcgt taccgaactc ttccgccgat cgacgaaacc
     1501 cgccaatatg tccgcaaggt cttgcggtat tatcgactct tcctcgcccg tgaccttggc
     1561 gtgaccggcc acatcatccc gtcttcggag tatgccgtgc cacgacccgc ttcggtgtct
     1621 tcaaatcagc ctggccggta gtgcgaatcc ccaaacacac ttgactatgc tgtttccagc
     1681 ccggacgttt ggatcccgga aaatcagatc gataatgggc accgacgctg ttttccctcc
     1741 agagtgcggc ttccgccacg caatgcgcca cttgaaccat gttcttgacc tctagatcgg
     1801 ctctccccgc aaaggatctg gacaccatcc gttcccaccg cgcaagctga gccgtggcgc
     1861 gaacaagcga ctcccgagaa cggacgagcc ccacctgtcc ccacatagtc cgtcgaagag
     1921 agctgcgtac tttttcagca tcctccaatc gttccaaccg accgcgcctc aaacgctcca
     1981 catggtgagc caaatcgggc atcgaacgac gagagatcca agctacggcg gcaacaccgg
     2041 cccgcattcc aaacaccaat ccctccagca atgagttgct cgccagtctg ttcgcaccgt
     2101 gcacaccgct gcaagccacc tcgcccgccg caaaaagacc cggcaatgtc gtggccccgt
     2161 cgatgtcggt cgcaacccct cccatcatgt aatgagcgct cggagagacc gggatccatt
     2221 cttccgtgat atcgatatca tggcgtagac aagtcgcata gatcgtcgga aatcgccgtt
     2281 tgacaaaatt cgatcccaag tgagtcacat caaggt
//
LOCUS       DATNDD010000390         4005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1077897, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000390 DATNDD010000000
VERSION     DATNDD010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077897"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VN647_09480"
     CDS             complement(<1..1345)
                     /locus_tag="VN647_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWG97302.1"
                     /translation="MADRSIGGCCGSQHQGANDMSKQIPMTQISSHTGTDIDPVCGMR
                     VDPATAAGHYNYQGKTYYFCAVSCLDTFKADPERFLTSAPADLISLGKKKPLPMMMPG
                     QPAPAIGEIDPVCGMAVQPATAAASYEYCGKKYYFCAARCLEKFRADPEYYLTPPEQR
                     TPKPTPVSASEITKYVCPMDPEVLETKPGACPICGMALEPADVTAASTRTEYTCSMHP
                     EIVQAEPGNCPMCGMALEPRTVAAEEVNPELVDMTRRFWQSVVLGSPILALMISEIMP
                     GQPLQHLFSGKVLIWFQFVLATPVVLWTGRPLFERAWASVVNRHLNVFTLIGLGTGAA
                     YFYSVAATLVPGMFPDSFRVHGGELAVYFEPAVAIIALVLLGQVLELRARSRTSSALK
                     ALLGLAPKTARIIRGDAREEDIPLEQVQVGDRLRVRPGEKIPVDGVVLEGSSAVDE"
     gene            complement(1489..2046)
                     /locus_tag="VN647_09485"
     CDS             complement(1489..2046)
                     /locus_tag="VN647_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWG97303.1"
                     /translation="MQEARTNGVMQRLLENESAFRQFIHRRVGDDVVVEDILQQSLTK
                     AVERSHSLNNEQSALAWFYRILRHTVADYYRSHGAETRRNEAVLKELTISGNHQEPPF
                     DELQATACTCLHGLLPSLHGNYAELIKRIDLDGESPVQVAKELKISRNNLTVRLHRAR
                     QSLRASLEDACGICSRHGCLNCTCG"
     gene            complement(2063..2719)
                     /locus_tag="VN647_09490"
     CDS             complement(2063..2719)
                     /locus_tag="VN647_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWG97304.1"
                     /translation="MNGPMTSRWKVALVAMGTIGLLNAPVIAQAAKPELKGKFEILKD
                     EPSTHQPGKVKVVEFADFYCPHCHHFEETGVPLLLKEFGNRVDITMVGFPVIPGKLPT
                     VFDMYEQAKLMGKGDQMRAILFRTIHKDKVDGVFDRSIRSLLIKEAGLDVTAFETGLE
                     SGKPARLFEEGRRLGERIKVSSTPSLLLDGNIKVDGANMTPENVVTIIRSILDADAKK
                     "
     gene            2801..3346
                     /locus_tag="VN647_09495"
     CDS             2801..3346
                     /locus_tag="VN647_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWG97305.1"
                     /translation="MRLFSYAGLVSAFVIFTGCALTGDQAEPLPSGAPSPSITYEQVK
                     AAPESYNGQPVTFGGKVLGARRLKEGTRIEILQLPLTGSLRPTVDLGKSQGRFVALQK
                     DFLDPATVPPGTFVTVTGEMAGSVTLPLDETEYTYPLVRINNLRVWTEDDEESPRIRR
                     PIGPYWSPYWSPYWHSWPYYW"
     gene            3657..>4005
                     /locus_tag="VN647_09500"
     CDS             3657..>4005
                     /locus_tag="VN647_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWG97306.1"
                     /translation="MMIRNDRAVKGKPSNLSSSIVAALKEQIIHWQYPPEHRLTEAEL
                     CEKFNVSRSPVREALRVLASDGFVKKMPNRAYVVRQYNLGEIEELYDLRLALELYTVE
                     RLATKDPLHRNDRE"
BASE COUNT          890 a         1214 c         1023 g          878 t
ORIGIN      
        1 attcatcgac agcacttgag ccctccagca ctactccatc gaccggaatc ttttcgcccg
       61 gtctgacacg caaccgatcg ccgacctgca cctgttccaa cgggatatct tcttcgcgag
      121 catcaccgcg aatgatacgg gccgttttcg gcgcgagtcc tagcagggcc ttgagggcac
      181 tgctggttcg gctacgggcc cgcagctcca acacctgtcc taataagaca agagcgatga
      241 tggcgacagc cggctcaaag tagacggcaa gctcgccccc gtggacacgg aacgagtcgg
      301 gaaacattcc aggaacgagc gtagcggcga cactatagaa ataggctgcg ccggtgccga
      361 ggccgatcag agtaaacaca ttgagatggc gattgacgac cgaagcccag gcccgctcga
      421 acagcggccg gccggtccag agcaccacag gagtcgccag cacaaactgg aaccaaatca
      481 atacttttcc tgaaaagagg tgttgtaaag gctgtcccgg cattatctcc gaaatcatca
      541 gcgccaggat aggtgaaccg aggaccacgc tctgccagaa ccgcctggtc atatccacca
      601 gttcaggatt cacttcctcc gccgccaccg tgcgaggctc taacgccatc ccgcacatcg
      661 gacaatttcc aggctcggct tgcacgatct caggatgcat agagcaggta tattccgtgc
      721 gcgtactcgc agccgttaca tcagccggct ccaatgccat accgcatatg ggacatgctc
      781 caggttttgt ctcgagcacc tcggggtcca tcgggcaaac atacttcgtg atttcgctcg
      841 ccgatacggg cgtcggtttc ggagtccgtt gttccggtgg ggtgagataa tattcgggat
      901 cggctcggaa cttctcgaga cacctggccg cacagaaata gtatttctta ccgcaatact
      961 cgtacgaagc ggccgcggtg gcaggctgta ccgccatccc gcacaccggg tcgatctcgc
     1021 cgattgccgg cgccggctga cccggcatca tcatgggcaa cggctttttc ttccccaatg
     1081 aaatgagatc ggccggcgcc gacgtcaaga atcgttccgg atcggctttg aatgtatcga
     1141 gacaggatac cgcgcagaaa tagtacgtct tgccttggta gttgtaatgc cctgccgcag
     1201 tggcggggtc caccctcatc ccgcataccg gatcgatgtc cgtcccagtg tgcgaagaaa
     1261 tttgagtcat cggtatttgc ttgctcatgt cgttcgctcc ctgatgctga ctcccgcaac
     1321 agccgcctat cgatcgatcg gccatggtcc gtctcctttc atcttatccg gtctgcttgc
     1381 tcaatgtcgc cttcacccct aaagacggac cagggcgccc aatattacag agagccggcc
     1441 ggcacgattg aaaacgatca gaaaagaggc agagacagga gaggacggct agccacaggt
     1501 gcagttcaag cagccatgtc tactgcagat tccgcaagcg tcctccagag aagcccgtaa
     1561 ggactgacgg gctctgtgca aacgaaccgt gagattgttc cgcgagatct tcaactcttt
     1621 cgcgacctgt accggtgatt ccccatcaag atcgatgcgt ttgatgagtt ccgcataatt
     1681 cccatggagg ctcgggagga gaccgtgaag gcaggtgcaa gcggtcgcct gtaactcgtc
     1741 aaacggcggt tcctgatgat tgccggaaat ggtcaattct ttcagtacgg cctcattccg
     1801 acgagtctcg gctccatgag aacgataata atcggccacc gtatggcgga ggattcgata
     1861 aaaccaggcg agcgcacttt gctcgttatt caacgaatgg gagcgctcga ccgctttggt
     1921 gaggctttgt tgcaggatat cttccacgac aacgtcatcg ccgactcgac ggtgaataaa
     1981 ctgtctgaag gcggactcgt tctccaaaag tcgttgcatg accccgttcg tcctcgcctc
     2041 ctgcatcctc gctccccacc cgtcacttct tcgcatcggc atcgaggatg cttcggatga
     2101 tggtgacgac gttctcaggc gtcatgttgg ccccatcgac cttgatgttc ccgtccaaca
     2161 aaagcgatgg agtggacgac accttaatgc gctctcccaa acgacgccct tcttcgaata
     2221 gcctggccgg cttcccactc tccagcccgg tctcgaacgc cgtgacgtcc aggcccgcct
     2281 ctttgatcaa caatgaacgg atggagcgat cgaacacacc gtcaaccttg tctttatgaa
     2341 tcgtacggaa caagatcgcc ctcatttgat cgccctttcc catcagtttc gcttgctcat
     2401 acatatcgaa cacggtcgga agttttcctg ggatgacggg aaatccgacc atcgtgatat
     2461 ccaccctgtt cccgaactct ttcaggagaa gcggcacgcc ggtttcctcg aaatgatggc
     2521 aatgcggaca gtagaagtct gcgaactcga cgactttcac cttccccggc tgatgggttg
     2581 acggctcgtc tttgagtatt tcgaacttac ccttcagctc cggcttggcc gcctgcgcaa
     2641 tcaccggcgc gttgagcagc ccgatcgttc ccatcgccac aagagccact ttccacctcg
     2701 acgtcattgg accattcatt tcgtactcct tccacaggta gatagccgat gtcattatag
     2761 cggatagagc gcgacctttc ttctttgata tactctgcgg atgcgcctat tctcctatgc
     2821 aggcctcgtc tccgcattcg tcatcttcac aggctgtgct ttaaccggtg atcaggccga
     2881 gccgctacct tccggcgcac cttcaccttc catcacctat gagcaggtca aagccgctcc
     2941 cgaatcttac aacggacagc cggtcacatt cggcgggaaa gtgctgggag cacgtcggct
     3001 caaagaggga accagaatcg agatactcca attgccgttg accggctccc ttcggccgac
     3061 agtggacctc ggcaagtccc aaggtcggtt cgtggcccta cagaaggact ttctcgaccc
     3121 tgcgacggtt ccgcccggca cgtttgtgac cgtaaccgga gagatggccg gctccgtcac
     3181 cttgccgctc gatgaaacgg aatacaccta cccgctcgtg cgcatcaaca atctgcgcgt
     3241 gtggaccgaa gacgacgagg aatcacctcg cattcgccgt cccatcggcc cctattggag
     3301 cccttactgg tctccgtact ggcactcttg gccctactat tggtaagctg gcgcgcggga
     3361 ctctcggcac cgtgataggc aagtctcaag ccgactattc attctctctc tcacccgcct
     3421 tgtccatcac cgtaaacgat gtccacgtgt ccaccttatt cccgtatcac cctctcgaca
     3481 tcttcatttc tgcgccggcg accggcgata tgccatccat gacacattcg acttggtacg
     3541 cttaattgta tacaatccat agagttgccg ccgctgaaga tgaaaccgac ttgtctgccc
     3601 gcacatgacg gagcgtcgat cataaacttt tctgaagctc agggtcccga aagaacatga
     3661 tgattcgaaa tgaccgagcc gtgaaaggaa aaccatccaa ccttagttca tcgattgtcg
     3721 ccgcactgaa ggaacagatc attcattggc agtacccacc tgaacatcgg ctgactgaag
     3781 ctgagctgtg cgaaaaattc aacgtgagtc gaagtccggt gcgggaagcg ctccgtgtac
     3841 tggcatccga tgggttcgtg aaaaaaatgc ccaatcgggc ctatgtcgtc agacaataca
     3901 acctcggtga gatcgaggag ctatacgacc ttcgcttggc gttggaactc tacaccgtcg
     3961 aacgcttagc gacaaaggat cctctccacc gaaacgatag ggagg
//
LOCUS       DATNDD010000391         2703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2192377, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000391 DATNDD010000000
VERSION     DATNDD010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192377"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VN647_09505"
     CDS             <1..840
                     /locus_tag="VN647_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG97307.1"
                     /translation="RFIPESPRWLMTHGRVPDAEVIVSQIERQVAQDRGISLAEPQGT
                     ITVHVRPHATLATVVRELFHSYPRRTVLGIGLMITQSFMYNAVSFTYPLLLTKYHAVP
                     SADVGLYILPFALGNFLGPLLLGRLFDTVGRKPMISLTYGISGSLLWSTGYLFWTGSL
                     TLTSHMLWWSSIFFFASAGASAAYLTVSEIFPMEIRAMAVAFFFIVAQGAGVAAPWLY
                     GMLIETSATSVFYGYLVAGGMMLLGAAIELWLGINAEGQSLEQLATPLSARSSPGGPD
                     RSA"
     gene            complement(907..1680)
                     /locus_tag="VN647_09510"
     CDS             complement(907..1680)
                     /locus_tag="VN647_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWG97308.1"
                     /translation="MFDWMTHSEMWIALGTLTALEVVLGIDNIIFISVLVGRLPQRQR
                     NVARRLGLGLAMVARLGLLFSISLMMELTAPLFTVLNQAVSGRDLILIIGGLFLLIKA
                     THEIHNSLEGTDEQDLSTVPAGLGMMLLQIALLDIVFSLDSVITAVGLVDEVSVMATA
                     IIAAVLLMMFAARPIGDFVDAHPTIKILALSFLILVGVTLMVEGFDVHVPKGYIYFSM
                     AFSVAVEMINLRIRARATPPIKLHNRYSRGKAGSTAQAD"
     gene            2266..>2703
                     /locus_tag="VN647_09515"
     CDS             2266..>2703
                     /locus_tag="VN647_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HWG97309.1"
                     /translation="MGTVDEMVGASPAICAVFDLVRKMATTEMPVLITGETGTGKELT
                     AQAIHERSLRKHGPFVPINCGAIPETLLESELFGHERGAFTGAVQQKKGKLEAAAGGT
                     LFLDEVGDLLPALQVKLLRFLQEGTFERVGGQEMLHVDARVLAA"
BASE COUNT          646 a          703 c          748 g          606 t
ORIGIN      
        1 cggttcattc ctgaaagtcc tcgctggctc atgacccacg gccgggttcc ggatgcggaa
       61 gtcattgtgt cgcagattga acgacaagtg gcgcaagacc gaggcatatc actcgcggaa
      121 ccacaaggca cgattacagt acatgtccgc cctcatgcga cgctcgcgac cgtggtacga
      181 gaactatttc actcgtatcc acggcgaaca gtcttaggta taggattgat gatcacgcaa
      241 tcgttcatgt acaacgccgt atcgtttacc tatccgctgc tgctgacgaa ataccatgcc
      301 gtgccgagtg ccgacgtcgg tctctatatc cttccgttcg cactgggaaa ttttttaggg
      361 ccgctcttgt taggtcgtct gtttgatacg gtcggacgca aaccaatgat cagcctcacg
      421 tacggaatct ccggtagcct tttatggtcc acggggtatc tattctggac aggctccttg
      481 acgctcacct cgcatatgct gtggtggtca tcgattttct ttttcgcctc ggccggggcc
      541 agtgccgcct atctgacggt gagcgagata tttccaatgg agatccgcgc gatggcggtc
      601 gcctttttct tcatcgtggc acaaggcgcc ggcgtggcgg cgccgtggct ctacgggatg
      661 ctcatcgaaa catcagccac gagtgtcttc tacggatatc ttgtggccgg aggcatgatg
      721 ctcctcggtg cggcaatcga gctgtggctg ggaatcaatg cggaagggca gtcactcgag
      781 caattggcga cgccgttgtc ggcgcgcagc tcacccggag gcccggatcg gtcggcctaa
      841 ccggttcgct gtggccggct tccaatatga gccgattcgg ttggatgatc ggtatgaacc
      901 tccttatcaa tctgcttggg ctgtggatcc cgctttgccg cgcgaatacc ggttgtgcag
      961 cttgatcggc ggtgttgcgc gtgctcggat tcgtaggttg atcatctcga cagcgacgga
     1021 aaatgccatc gaaaaataga tatagccctt gggcacatga acatcaaaac cttccaccat
     1081 caaggtcacg ccgacaagga tgaggaacga cagcgctaag attttgatcg tgggatgggc
     1141 atcgacgaaa tcaccgatgg gccgagcggc gaacatcatg aggagtacag ccgcaatgat
     1201 cgccgtcgcc attacggaca cctcatcgac caatccgacc gccgtgatga cggaatccaa
     1261 cgagaagacg atatccagca gagcaatctg aagcagcatc attccaaggc cggctggaac
     1321 ggtcgagagg tcctgttcgt ccgtcccttc caggctgtta tggatttcgt gagtcgcttt
     1381 gatgagaaga aacagtccgc cgatgatgag aatcagatct cgccccgaga cggcttgatt
     1441 cagcaccgtg aacagaggag ccgtcagctc catcatgagt gaaatggaaa ataacaagcc
     1501 cagtcgtgcg accatcgcaa gaccgagccc aagacgccga gccacattcc gttgacgctg
     1561 aggcaggcgc cctacaagga ccgaaataaa aataatgttg tcgatcccca acacgacttc
     1621 gagggctgtc aaggtgccaa gcgcgatcca catctctgaa tgagtcatcc aatcgaacat
     1681 gcgattctcc agtaagcaga aagcaacagg ttcagaatcc gctccgggca aggatccttc
     1741 ttcagcacga taggtcgaca gggaggtcaa tataatcgtg gtttggtatg tcgtggaacc
     1801 ttaaggtcca tctacaaact actttatgaa caatacttaa tctcttgctt cacggtcatc
     1861 ctgacgactc ctcgttcaat ccatgattaa cgagctagat ctaaaaggcc ctaatcaaag
     1921 gatatgaaga agggccatta cgcactaacc gtccctatgg gccaaatgct attttttaga
     1981 agagtgcctt gaatcttgtt gagcggcatg gcgccatcct gaaaatcaga gtacatgtag
     2041 aactgtgcag gtatttttac tagatacgta atgtcaatct ctggtatacc agagatccgt
     2101 atgtatggaa tgctcaattc atcaggcatg agtcgaaatg tcctacgctg ggatctttca
     2161 aggctggtga aggcagaacc atgtcggatc gtatagtcta catatatttc atgaaaggca
     2221 agatccaggg aggtcggtgt aggaatctac actagggagg tgcttgtggg aaccgtggac
     2281 gaaatggtgg gagcgagtcc cgccatctgc gcggtctttg acttggtccg caagatggcc
     2341 accaccgaaa tgccggtgct cattaccggc gagacgggca cgggcaagga attaaccgcg
     2401 caagcgatcc atgagcgaag cctccgaaag cacgggccgt tcgtaccgat caattgcgga
     2461 gccatcccgg aaaccttgtt ggaatcggaa ctgttcggtc atgaacgcgg ggccttcaca
     2521 ggggcggtcc aacaaaagaa gggcaaacta gaagcggcgg ccggcggcac attatttctt
     2581 gatgaagtag gcgatctgtt gcccgccctc caggtcaagc tgcttcggtt tttgcaggag
     2641 ggcacatttg aacgggtcgg gggacaagag atgctccatg tcgatgcgcg agtccttgcc
     2701 gcg
//
LOCUS       DATNDD010000392         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1916390, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000392 DATNDD010000000
VERSION     DATNDD010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916390"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /gene="glgC"
                     /locus_tag="VN647_09520"
     CDS             <1..1245
                     /gene="glgC"
                     /locus_tag="VN647_09520"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWG97310.1"
                     /translation="RRVKNIFTMVLAGGKGERLFPLTDQRAKPAVPFGGKYRIIDFTL
                     SNCLNSGFRKIVVLIQYKSHSLDRHIRTGWNILNAEVGECIASVPPQQRINEDWYRGT
                     ADAVYQNLFLIEGEHPEFLLVLAGDHIYKMNYAEMFHWLIAKSADAIVGAIDIPVQEA
                     NRFGVIAVDEDYRITRFDEKPANPIPLPNDPAHAFGSMGIYLFRTKAIREHLIADARE
                     DSAHDFGKNIIPRMIEQGRVYAFKFQDANKKAVQYWRDIGTLDAYWEANMDLVAVDPQ
                     FNLYDSEWPIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIVSGGRIQNSVLS
                     PNVRVQDHADVRESIIMENVTIGAQSRIRRAIIDKDVTIPPHTEIGYHREADAQRFTV
                     TESGLVVISKGMKLHAAVDPSG"
     gene            1220..3079
                     /locus_tag="VN647_09525"
     CDS             1220..3079
                     /locus_tag="VN647_09525"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWG97311.1"
                     /translation="MPPSIHPVDLITALRHKHLTPAIVFLTSRRACDEAMETFDHADL
                     VLPPARQEAIAVALERITIQHPSIAEHPLIPIVRRIGVAAHHAGHLPSWKIAIEELMR
                     QGHLDAVFATTTLAAGVDFPARTVVITQSSIRKSRDFTDLAIGEVQQIAGRAGRRGKD
                     HVGFAIVTPSPYIDLAVLTKGLTGQPEAINSQFTISYPMVLNLLKAHPHEHIQGILAK
                     SFAQFQLNQRAELLEQKLDAVHVQMEPFGPRVCTDWITQWHTFDHARRHRHTRHQTHR
                     SEPPEISARLPFLTPGRVVGLGRGRGIVLRQYRSKGQKNSMLTVLRPDSAVTECPVTG
                     VKEVYDRTYDCAETPTYPWCSTETFDRLSHQLEDLPSHVPVLPILISPGIEPLPDAIV
                     QSLGDFPCPTCPSRPACQKDFITASRLRQEQQRHTKSIQALRTSLWHRFQERVEVLQQ
                     FGYLTTATQLTIDGEWARLIRIDHSLLITELIRAEAFTGADPSLLAGVLASLAHDDDR
                     PGAFPRISPGLSSLLGQVRKLAESVSPYEDPPLLRADVAALVERWVADPTLTWIGLCR
                     LTTMAEGDIYRLLARTLEFLSQVQALKSTHPGLAESASQAITLIRRGVLEELP"
     gene            3150..>3389
                     /locus_tag="VN647_09530"
     CDS             3150..>3389
                     /locus_tag="VN647_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97312.1"
                     /translation="MTTEELEKLLAQQPVVLLHRLARGRVHRHFRAGKRRLIELLLQH
                     SSQNRAGLESDLQALIGEQYPRSEAGSSCIHHPPVI"
BASE COUNT          734 a         1046 c          904 g          705 t
ORIGIN      
        1 cggcgcgtga agaatatttt cacgatggtc ttggccggcg ggaaaggcga gcgactcttt
       61 ccgctgacgg accaacgtgc gaagccggct gttcctttcg gcgggaagta tcgcatcatc
      121 gactttacct taagcaattg tttgaactcc ggattccgta agatcgttgt tctcattcaa
      181 tacaagtcgc actcgctcga tcgccatatc cggaccggct ggaatattct caacgctgaa
      241 gtcggcgagt gtatcgcctc ggtccctcct caacagcgca tcaacgaaga ttggtaccgc
      301 ggcaccgccg acgccgtgta tcagaacctg ttcttgatcg agggggaaca cccggaattc
      361 ctgttggttc tcgccggcga ccacatttac aagatgaact atgcggagat gttccattgg
      421 ctcatcgcca agagcgcgga tgcgatcgtc ggcgccatcg acattcccgt ccaggaggcg
      481 aatcgcttcg gagtcatcgc agtggacgaa gactaccgga ttacccgctt cgatgaaaag
      541 ccggcgaatc ccattcctct ccccaacgat cccgcccacg cctttgggtc gatgggcatc
      601 tacctgttcc gcacaaaggc gatccgggaa catctgatcg ccgacgcgcg agaggacagc
      661 gcgcatgatt tcgggaaaaa tatcattccc agaatgatcg aacaaggtcg tgtctatgcc
      721 ttcaaatttc aagatgccaa caaaaaagcc gtccagtact ggcgcgacat tggaacgctc
      781 gacgcttact gggaagcgaa catggatttg gtcgccgtcg atcctcaatt caacctgtac
      841 gactctgaat ggccgatccg aacctatcag ggacagtttc cacccgccaa gttcgttttc
      901 gcacaggatt ttcagggagg acggatgggc gtcgccctcg attcgatcgt ctgcggcggc
      961 tgcatcgttt cgggcgggcg gatccaaaat tcggttttgt ccccgaacgt ccgcgtgcaa
     1021 gaccatgcgg atgtccgtga atccattatc atggaaaacg tgaccatcgg cgcgcagagt
     1081 cgcatcagac gcgccatcat cgacaaggat gtgacgatcc ctcctcatac ggaaatcggg
     1141 taccatcgag aggccgacgc gcaacgattt accgtcactg aatcaggcct cgtggtcatc
     1201 tcaaagggaa tgaaactgca tgccgccgtc gatccatccg gttgatctca tcactgcgct
     1261 ccgtcacaag catctcacgc cggccatcgt gtttctcacc tcacgccggg cctgtgacga
     1321 ggccatggag acctttgacc atgccgatct cgtgctccct ccggcacggc aggaggcgat
     1381 cgcggtcgcg cttgagcgga tcacgattca acaccccagc attgccgagc acccgttgat
     1441 tcccattgtg aggaggatcg gggttgccgc tcatcatgcc gggcatctgc cttcttggaa
     1501 aatcgcaatc gaggagctga tgcggcaagg ccatctcgat gcggtattcg ccaccacgac
     1561 cttggcggca ggtgtcgatt ttcccgcccg cacggtcgtg attacccaat cgagcatccg
     1621 caagtcccgt gacttcaccg acctggccat cggcgaagtc cagcaaatcg ccggacgagc
     1681 cggccgccgc ggaaaagatc atgtgggatt tgcgatcgtg actccctctc cttatatcga
     1741 tctcgcggtt ctgaccaaag ggttgacggg acagccggaa gccatcaaca gccagtttac
     1801 catcagctat ccgatggtct tgaacctctt gaaggcccat ccccatgagc acattcaagg
     1861 aatcttagcc aagagtttcg ctcaattcca actgaatcag cgcgcggaac tcctcgaaca
     1921 aaaactagac gcggtccatg tgcagatgga accgttcggt cctcgcgtct gtacggactg
     1981 gatcacccaa tggcacacct tcgaccatgc gcgaagacat cgccacacgc gacatcagac
     2041 gcatcgatca gaaccgcctg agatttctgc ccggttgccg ttcctgacgc cggggcgtgt
     2101 cgtaggactc ggcagaggcc gcgggattgt gctccggcaa tatcggagca aaggccaaaa
     2161 gaattccatg ctcaccgtct tacgaccgga cagcgccgtc accgaatgtc ccgtcaccgg
     2221 cgtgaaagaa gtttacgatc gcacgtacga ctgcgcggag acgccgacct atccttggtg
     2281 ttctaccgaa acgttcgacc gactcagcca tcaattagaa gatcttccgt cgcatgtgcc
     2341 cgtgcttccc atactcatat ctccgggcat cgagccgttg cccgatgcca tcgtccaatc
     2401 gttaggtgac ttcccctgcc cgacttgtcc ttcgcgacca gcctgccaga aagacttcat
     2461 aaccgcatcg cgccttcgcc aggaacaaca gcgccatacg aaatccatac aagcgctacg
     2521 gaccagtttg tggcaccgct ttcaagagcg cgtggaagtg ctgcagcaat tcggatatct
     2581 caccacggcg acacagttga cgatcgacgg agaatgggca aggctgatcc gcatcgacca
     2641 ttcgctgctg atcaccgaac tgatccgcgc cgaagccttt accggagcag acccgtccct
     2701 cctcgccggc gtcttggcca gtctggccca tgacgatgat cgacccggcg cgtttccacg
     2761 catcagtccg gggctgagtt ccttgctcgg gcaggttcgc aaattggcgg agagtgtgtc
     2821 tccttacgaa gatcctcccc tcctacgcgc cgatgtggcg gcgctggttg agcgctgggt
     2881 cgccgacccg accctcacat ggatcggact ctgtcgactg accacgatgg cggaaggcga
     2941 catctatcga ttactggcca ggaccttgga atttctgtca caggtgcagg ctttaaaatc
     3001 tacgcacccc ggcctcgctg aatccgcttc gcaagcgatc acattgatcc ggcggggagt
     3061 gttggaggaa ctgccatgag ccgtgtagga ctgagggccg aggcttcagg actgagctgt
     3121 cctgcgacca gtcctcagca cttccaatta tgaccaccga agaacttgaa aaacttctcg
     3181 cacaacagcc cgtggtgctc ttgcatcgcc tggcccgtgg acgcgtccat cggcactttc
     3241 gtgccggcaa gagacgactg atcgagctgc ttcttcagca ctcaagccag aatcgtgccg
     3301 gtctcgaatc cgatctccag gccttgatcg gggagcaata cccccgatcg gaagccggat
     3361 cgtcctgcat ccatcatccg ccggtgatc
//
LOCUS       DATNDD010000393         4012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2448918, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000393 DATNDD010000000
VERSION     DATNDD010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448918"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /gene="coaBC"
                     /locus_tag="VN647_09535"
     CDS             complement(<1..1210)
                     /gene="coaBC"
                     /locus_tag="VN647_09535"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWG97313.1"
                     /translation="MDEAQSPIHLWGKRLVLGVTGSIAAYKAVGLLRALAREGATVSV
                     VMTQAATKFVTPLTFEVLSGRRVATDLFESHEEMAHLAVPGQAHAVIVAPATANFLAK
                     AALGLADDLLSTILLNARCPVIVAPAMDGDMWTHPTVVQHVHALRTRGVVVLDPEVGP
                     LASGQVAQGRLSAESRILDALHAALIPQQDWQGQRVLVSAGPTHEPIDPVRFISNRSS
                     GKMGYAIAEAARDRGAEVVLVTGPSSLTPPPGVTTVSANTASEMSDALSRHFPSSTVL
                     IMAAAVADFRPKMPAGQKLKKHGKSELILDLEAIPDILAMLSARRTSQIMVGFAAETE
                     QVLSYAKDKLREKGLDLIVANDVAQAGGGFGSDDNAVVIFSTTGEQKVLGLMPKRRLA
                     DEILTAVRELC"
     gene            complement(1214..1573)
                     /locus_tag="VN647_09540"
     CDS             complement(1214..1573)
                     /locus_tag="VN647_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWG97314.1"
                     /translation="MIDMLSLLPQYTSNEFDSRHRLVIVASQRAKHLTQGAKQAVSSR
                     FTKETTIALDEVLRGHAKYLTGKEARDAMKEAKRGKEGETERIAMMTGEDAREIKKEL
                     GVYVDDTVQPTAAPAEE"
     gene            complement(1593..2309)
                     /gene="gmk"
                     /locus_tag="VN647_09545"
     CDS             complement(1593..2309)
                     /gene="gmk"
                     /locus_tag="VN647_09545"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWG97315.1"
                     /translation="MNTAITTSNHPPGVTGNRQVPERRGILYIISAPSGAGKTTLCKQ
                     VVASVSGLWHSVSFTTRKPRPGEEHGREYFFIDEKVFHDMVARNEFLEYAHVYSNWYG
                     TPRKPLMDKMEQGIDVLLEIDVQGALQIKKKFGDAVYIFILPPSMEILRARLQGRGSD
                     SQEEIVRRLQKVKEEVWSFREYYYIVRNDDLTQSLHELQSIFVAERLKTKRMDMHWLE
                     QSFILEKDAKLPEREPSSTL"
     gene            complement(2306..3184)
                     /locus_tag="VN647_09550"
     CDS             complement(2306..3184)
                     /locus_tag="VN647_09550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWG97316.1"
                     /translation="MIKSMTGFGRRQGTWSDGTVSVEVRSVNHRFLETSIRIPKWMGG
                     LEERFKKAIQQHCGRGRVDLTVLLQGGRGSARAVQLDVELAKQYHHALLTLQRTLKLK
                     GSIDIGLVAGFRDIIALSEQPIDDPKLARVVEKLGLLAVSDMTKMREKEGVLLAQDIL
                     ARLDQVRECRSAVSSRVPYMAQETFDRMKQRVEKLLADSIPDLPRLNQELALYADRCD
                     ITEELVRLDTHMVQFERVLQGTEPVGKTLDFLLQEMGREVNTIGSKANDAAIRANVVR
                     MKAELERMREQIQNVE"
     gene            complement(3181..3846)
                     /gene="nth"
                     /locus_tag="VN647_09555"
     CDS             complement(3181..3846)
                     /gene="nth"
                     /locus_tag="VN647_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWG97317.1"
                     /translation="MKKVKRMGGQSTLDRPAQIAMSLHHAMPVARVELTHRSPWELLV
                     ATILSAQCTDQRVNQVTPSLFTQYPTPQAMAKAMPTELEALIRSTGFYKSKAKNLIGC
                     AQAITAQFKGRVPDAMEELTSLPGVGRKTANVLIGAVFGKPAIVVDTHVGRVANRLAL
                     THSRDPEQIERDLQSVYPQTQWTGVSQRLLLHGRYVCLARKPRCRVCPIYDVCEWEGK
                     LQK"
BASE COUNT          880 a         1175 c         1001 g          956 t
ORIGIN      
        1 gacacagttc gcgcacggca gtcagaattt catcggccag acggcgcttg ggcatcagcc
       61 ccaagacttt ctgttcaccc gtggtcgaga aaatgacgac cgcattgtcg tcactgccaa
      121 acccacctcc cgcttgcgca acatcgttgg caacgatcag atccaagcct ttctctctca
      181 acttgtcttt cgcataagac agtacttgtt cggtttccgc cgcgaaaccg accataattt
      241 gcgatgtccg acgcgcggac agcatcgcga gaatgtccgg aatggcttca agatcgagga
      301 tgagctcaga tttgccatgc tttttgagct tctgcccggc cggcattttg gggcgaaagt
      361 ctgcgactgc tgcagccatg atcagaaccg tagaggacgg gaagtgccga ctcagcgcgt
      421 cgctcatctc actcgcagtg ttggctgaaa ccgtggttac tcctggagga ggcgttagag
      481 aggaaggccc tgtaaccagg acaacttccg ctcctcgatc ccgtgcggct tctgcaatgg
      541 cataccccat ctttccggag gaacggttgg aaataaaccg aaccggatca ataggttcat
      601 gagtcggtcc cgcggacacc aacacccgct gtccttgcca atcctgttgc ggaatcagcg
      661 ccgcatgaag ggcatccaag attcttgact ctgctgaaag ccttccttgt gcgacttgtc
      721 ctgatgccaa cggaccgact tcaggatcaa gcacaacgac gccacgggtt cgtagagcat
      781 gaacatgctg aacgaccgtg ggatgtgtcc acatgtcccc gtccatcgca ggagcgacaa
      841 tcacgggaca tcgagcattc agcagtatcg tactcaacag atcatccgcc agaccaagcg
      901 ccgcctttgc cagaaaattt gccgtagccg gcgccacaat aaccgcatgg gcttgtcctg
      961 ggacggcgag atgggccatc tcttcatgag attcaaagag gtccgtcgcg acccgccttc
     1021 cggaaaggac ttcaaacgta aggggagtca caaattttgt ggcagcctga gtcatgacca
     1081 ccgagacggt ggcaccttca cgcgcaaggg ctcgaagcaa accgaccgct ttatacgcag
     1141 cgatactgcc ggtcactcca aggacaagac gcttgcccca cagatgtatg ggcgattgtg
     1201 cttcgtccaa tccttactcc tcagccggag ccgccgtcgg ctgcaccgta tcatcaacgt
     1261 acacaccgag ctccttcttg atttcgcggg cgtcctctcc ggtcatcatt gctatacgct
     1321 cggtttcccc ctcttttccc cgtttggctt ccttcatcgc atcgcgggcc tccttgccgg
     1381 tcaagtactt ggcatgacct ctcagcacct cgtcaagcgc gatggttgtt tctttcgtga
     1441 agcgagagga cacggcttgt ttagcacctt gagtcaagtg cttcgcccgc tgtgaggcga
     1501 cgattaccag tcgatgacgc gaatcaaatt cattggaggt atattgcggc aacaaactca
     1561 gcatgtcgat cataataggc cttgccccct tgctataaag tggatgacgg ttctctttcc
     1621 ggcaattttg catctttctc aagaataaag ctttgctcaa gccaatgcat atccatccgc
     1681 ttggtcttca ggcgctccgc cacgaaaatg ctttgcagct catgaagaga ttgcgtcaga
     1741 tcatcgttgc ggacaatgta atagtattcc ctgaaactcc agacttcttc ctttaccttt
     1801 tgcaatcggc gaacaatctc ctcttgagag tctgaccccc gaccttggag tcgagcacgc
     1861 aggatctcca tcgatggagg gaggatgaag atatagacgg catcgccgaa tttcttttta
     1921 atctgaagcg ccccttggac atcgatttcc agcaagacat cgatgccctg ctccatctta
     1981 tccattaaag gcttccgcgg tgttccgtac cagttggaat acacatgtgc gtattccaag
     2041 aactcattcc tcgcaaccat atcgtgaaat actttctcgt cgataaagaa atattcacgt
     2101 ccgtgttctt ctcctgggcg cggttttctt gtcgtaaacg acaccgaatg ccacagcccg
     2161 gagaccgacg ccacgacctg tttacataag gtcgtttttc ctgctcctga aggggctgaa
     2221 ataatgtaca ggattccccg acgttcagga acttgccgat tccccgtaac acccggaggg
     2281 tggttacttg tggtaatcgc agtgttcatt cgacattctg tatctgttca cgcatacgct
     2341 caagctcggc cttcattcgc accacattgg ccctgatcgc cgcatcgttg gcttttgatc
     2401 caatggtatt gacttcccgg cccatctcct gaagaagaaa gtccaacgtc ttccctaccg
     2461 gttcggtgcc ttggagcacc cgctcaaact gtaccatatg cgtgtccagc ctgaccaatt
     2521 cttccgtaat gtcgcaccgg tcggcataga gagccagctc ctgattgagc cgaggaaggt
     2581 ccgggataga atccgccaac aacttctcca ctcgttgctt catacggtcg aaggtttcct
     2641 gcgccatgta gggaacacgg gacgatactg cactcctgca ttcacgcact tgatcaagtc
     2701 gagccaggat atcctgtgca agcaagaccc cctctttctc ccgcatcttc gtcatatccg
     2761 acaccgcgag taaccccagt ttctccacca ctcttgcgag tttaggatcg tcgatcggct
     2821 gctcggaaag tgcgatgata tcgcggaacc ccgccaccag tccgatgtcg atggagcctt
     2881 tgagtttcag cgtgcgctgg agcgtaagaa gagcatgatg gtactgcttc gctagttcaa
     2941 catcaagttg caccgcacga gcgcttcctc gaccgccctg tagcaacacc gtaagatcga
     3001 ctcttccgcg cccacaatgt tgttggatcg ccttcttgaa gcgctcttcc agcccgccca
     3061 tccacttcgg tatgcgaatt gatgtttcaa gaaaacgatg gttgaccgat ctcacttcaa
     3121 cactgacggt cccgtcggac catgtcccct gtctccggcc aaatcctgtc atactcttaa
     3181 tcatttctgg agttttcctt cccactcgca aacatcgtaa atcgggcaaa cacggcagcg
     3241 aggctttcgt gcgaggcaca cataccggcc atgaagaagc aaccgttgag acacgcccgt
     3301 ccactgggtt tgcggataca ccgattgcag atcgcgctcg atctgctcag gatctcttga
     3361 atgagtcagg gccagtcggt tggccacccg tcccacatgc gtatcaacca caatggccgg
     3421 ttttccaaat acggccccga tgagaacatt ggctgttttt cgcccgacac cgggtagcga
     3481 tgtgagttct tccatcgcgt ctggaactcg gcccttgaac tgcgcggtaa tggcctgagc
     3541 gcagccgatc aagttttttg ccttgctctt ataaaagccg gttgatcgga tcaatgcttc
     3601 caactcagtc ggcatcgctt ttgccatggc ttgcggtgtc ggatattgcg tgaataggct
     3661 cggcgtcacc tgattcacac gctggtccgt gcactgtgct gatagaattg tggcaaccaa
     3721 cagctcccac ggagaacgat gcgttaattc cactcgagcc accggcatgg cgtggtggag
     3781 actcatggca atctgcgcag ggcggtcgag agtggactgt ccccccatac gtttgacttt
     3841 cttcatggcg gcacgattgt gcagaggtta tcgacgagat gcaagcaccc gcgcgtcgtc
     3901 ctgcaacggc aagccgatag tctgatattc accggttggc agcgtcctca cgtaggcttc
     3961 cagtcgagcg aggagaggat gaagggtggt gagctggagc gccgaaatgc cg
//
LOCUS       DATNDD010000394        10034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2912718, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000394 DATNDD010000000
VERSION     DATNDD010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912718"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VN647_09560"
     CDS             complement(<1..394)
                     /locus_tag="VN647_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97318.1"
                     /translation="MIAQVPRIRRVGFYIAMLLISLLVIEAGFRAVLAFRVGPRVLLY
                     GTSSQRQLMPATVPADQVASKHTNTQQGYTKFFPNEVKVHFDNTTKEPFKISINSRGF
                     RGGEITEVKKPGITRIVTLGASSTFGYYD"
     gene            complement(591..1043)
                     /locus_tag="VN647_09565"
     CDS             complement(591..1043)
                     /locus_tag="VN647_09565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWG97319.1"
                     /translation="MTEMANIIVGKLWLYRLISHAYNHAWPKSKVGPVEAVLQEGTGW
                     RQSMAALLELVDMCRAHKILLVVFFERMDQNHDNLLLEDVVRHAEGVPAHDMASWFEG
                     FDESLLENSKIDRHPNAEGHRVMAEHMANDIVRCLVMTSPTAILASHK"
     gene            complement(1272..1637)
                     /locus_tag="VN647_09570"
     CDS             complement(1272..1637)
                     /locus_tag="VN647_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97320.1"
                     /translation="MLLGLLVVVALTLSLARSTWYQPIYQARTGLVISGVSFILSLGA
                     LELAVRPIDLLGISYYERLGDYIRDKVADDQLVNRHKPLWGTSYGNVRVSYNERGLRN
                     RPILPKAAEEYRVLPWETP"
     gene            complement(2511..3296)
                     /locus_tag="VN647_09575"
     CDS             complement(2511..3296)
                     /locus_tag="VN647_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HWG97321.1"
                     /translation="MDGRDTLNQKLSPLIELQKLDLRIMEISEIRRKIPERLHAAEAP
                     LREASRILNDTKVAVEAAVKERRSHEKDLEAHEAHTDKMKSHAASLKTNKEYQAHLFE
                     LELANKKRGDFEEKILLTMEKIDQLQNAAKELQEKKAALENVFTQEKQGLDAQDKEHA
                     TELAQLQLRHREASVRVEKALLDRYNQVKASRKDHPLAAVRDGICLGCRLQIPPQLIA
                     QVKRSEDLHLCPYCRRILYWEPIPEGIPASENKPSDLEVGESA"
     gene            complement(3332..3408)
                     /locus_tag="VN647_09580"
     tRNA            complement(3332..3408)
                     /locus_tag="VN647_09580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Ile,seq:cat)
     gene            complement(3565..5379)
                     /gene="rpoD"
                     /locus_tag="VN647_09585"
     CDS             complement(3565..5379)
                     /gene="rpoD"
                     /locus_tag="VN647_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWG97322.1"
                     /translation="MSKQELLGEVKKLITIGKEKGFLTYDELNSTLPAEVVSSDQFGS
                     IMAMFGEMDIEIIEASDGDRPRKRSEGEVGEDVEDVESDSEEENDKAIDLTPGALSRT
                     DDPVRLYLKEMGSVALLSREGEIEIAKRIEEGKNDIASVIYGMPMTIEFVLALRDQLK
                     NAKIDVREIVPIQETEEDFEEDQPPVERDYEELRVKTLESLNAVRKVSLALKAMAEKG
                     KHLGSDPAKQKKFKKQFDAIRQQVVNKIESVNLHGVLKDRMVQRVRDLAVQIRVSERE
                     AVSCQRRIGVGGEVGAELLRRMCRSRQDFLAVKRKTGVSEEALVEIRKVYQTAKAKIR
                     QLETEEALVPAEEIKDAVKHLDLAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFL
                     DLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETI
                     NKLIRTSRHLVQKLGREPLPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSH
                     LGDFIEDKKAVSPLEAAIRYDLQRQINSALETLTPREEKVLRKRFGIGEATDHTLEEV
                     GQDFEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL"
     gene            complement(5382..7223)
                     /gene="dnaG"
                     /locus_tag="VN647_09590"
     CDS             complement(5382..7223)
                     /gene="dnaG"
                     /locus_tag="VN647_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWG97323.1"
                     /translation="MGAATPVGRSLISDDIKNRIRDRVDIADVVGHHVSLRRAGQNLV
                     GLCPFHQEKSPSFSVSPSKQMFYCFGCKAGGDVFAFLTKITGATFPEVLKELGDKVGI
                     MVEESLAERVHRGQTHRLEEINRVAMAWFQANLRDPQIGATAREYLNGRGIQQSTVDS
                     FQIGVSSPEWDGLFKFLSRKGFSHSEIMTAGLGSPRPNGNGYYDKFHGRLMFTITDLR
                     KRVVGFGGRVLGDRMPKYLNSPDTLLFKKGQTLFAFDQAREAIVRTKTVIIVEGYFDA
                     IALHQAGLTHTVATLGTALTAEHVQVLRRFADQVVLLFDPDAAGVRAALRGLDLFVNS
                     GLGVKVVTLPVDEDPDTFVRKKGAVAFAQLEAAAPSLLDYALNHTVKQADDGSLESRI
                     RSVDEVLRILQKSEHPIERQERIRIVSERLGISETLLIERYPALLAQPKRGGEGPRAQ
                     ATQGISFNALFKGIPEERDLLLLLLHGKLSPADVSRLRPESFTVAPCRKLVEIALAHV
                     DREGCISLRSVLDESVDDPDCGALATELSMREDHFDDVQAHIVACLDCLDRKRSEQVM
                     RELIARLKTAEREGRMDEAHLLNMQINEVRMRKAATPTSGVASLVKE"
     gene            complement(7328..7672)
                     /locus_tag="VN647_09595"
     CDS             complement(7328..7672)
                     /locus_tag="VN647_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWG97324.1"
                     /translation="MSECLFCQIVEKKISAKLVQEDEHTLAFDDINPQAPVHTLVIPK
                     RHVSAVQDLGMGDDSLLARLLISCTKVAALKGVRESGYRLVANTGRDAGQTVSHLHFH
                     VLGGRHMAWPPG"
     gene            complement(7735..8403)
                     /gene="hisIE"
                     /locus_tag="VN647_09600"
     CDS             complement(7735..8403)
                     /gene="hisIE"
                     /locus_tag="VN647_09600"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HWG97325.1"
                     /translation="MSQEATDQFKFDGEGLLPVVIQDWLDGTVLMLGYMNQEAFAKTV
                     STKRVHFWSRSRNKLWEKGETSGHTLHVKELFLDCDCDTILVKAQPAGPTCHTGERAC
                     FFSKMDEQGHVVHRSAYDAQGGILESVLRIIRERRSSPQPGSYTSKLFEEGHDKILKK
                     VAEEAGEVLLASKGGKKEEIVHEVADLFFHTLMVLGYHDLPLQDIYEELGGRFGKPSL
                     RSGQ"
     gene            complement(8408..9187)
                     /gene="hisF"
                     /locus_tag="VN647_09605"
     CDS             complement(8408..9187)
                     /gene="hisF"
                     /locus_tag="VN647_09605"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWG97326.1"
                     /translation="MLTKRIIPCLDVKEGRVVKGVSFVNLRDAGDPVEAAVGYDHEGA
                     DELCFLDITASHENRKTIIDVVERTAARVFMPVTVGGGVGTLDDIRALLNAGADKVSI
                     NTAAVRRSEFVKEAARRFGTQCIVVAIDAKRATGDRWEVFTHGGRHATGLDAVEWAKR
                     MEEYGAGEILLTSMDQDGRQTGYDLNLTAAVSGSVSIPVIASGGVGALDHLYDGFVKG
                     KADAVLAASIFHFQTYTISQAKAYLRERGVPVRSDTLPRVA"
     gene            complement(9197..9919)
                     /gene="hisA"
                     /locus_tag="VN647_09610"
     CDS             complement(9197..9919)
                     /gene="hisA"
                     /locus_tag="VN647_09610"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWG97327.1"
                     /translation="MLIIPAIDLKDGCCVRLRQGDMAAETIYSDDVTEVAERWQQRGA
                     GLIHVVDLNGAVDGEPKNLPQIESIIKAVHVKIQVGGGIRTMDTVRRYLNAGVSRVVL
                     GTAALTNRTLLNLACREFPQRIVLGLDAREGRIAVKGWTTVSDVKAIDLLKELSGCAI
                     AAVVYTDIARDGMLNGPNLPALIEITEFSSFPVIASGGITSLEDLRAVQSIGPKIEGA
                     IIGKALYDGELDYQAAVAALSL"
BASE COUNT         2229 a         2985 c         2406 g         2414 t
ORIGIN      
        1 ggtcatagta gccgaacgta gacgaagccc caagcgtgac aatgcgggtg attccaggct
       61 tcttgacttc ggtgatttct ccgccgcgaa acccccgcga attgatggaa atcttaaagg
      121 gttccttcgt cgtattgtca aagtgaacct tcacttcatt tggaaagaac ttcgtgtaac
      181 cctgttgagt attcgtatgc ttactggcga cttgatcagc cgggaccgtt gcaggcatga
      241 gctgccgttg ggaactggtg ccgtagagaa gcaccctggg cccaacccga aaggcaagca
      301 cggcgcgaaa gccggcctca attaccaata ggctgatgag cagcatcgca atataaaagc
      361 caacccgcct gatgcgtggt acctgcgcaa tcatatcgct cctcttccat ttttcatcgc
      421 cacccgttga cacgagttac acccatcggt cgctaggaaa cctcataggc aaattgaacg
      481 ggaaaactgt tcgtccacag gttggaaagt cagacatcag catttcctgt ttgtcgatcg
      541 ccttaccggt ttgccctttc gttaaggagt atccacgctg tattgtccct ctacttgtga
      601 cttgccagta ttgccgtggg tgaggtcatg acgagacacc gcacaatgtc gttggccatg
      661 tgttccgcca tcacgcgatg gccttccgca ttggggtgcc tatcgatctt ggaattctct
      721 agtaatgatt cgtcgaatcc ttcaaaccat gatgccatat catgggccgg gactccttcg
      781 gcatgccgaa ccacatcttc gagtaacagg ttatcgtgat tctgatccat ccgctcgaag
      841 aacacaacga ggaggatctt gtgtgctctg cacatgtcca ccagttctag aagtgcggcc
      901 atagactgcc gccaccccgt tccctcctgt agcacagcct caaccggtcc gactttggac
      961 ttgggccaag catggttata ggcatgagag atcaatcgat agagccagag ctttcccact
     1021 atgatattgg ccatctcagt catcgatttt cctcggagag gacccctggc ccatggatcc
     1081 cacgccggat taagttcctc gatgtcattc tgcatgtgta ggtcaacatc accaaatccg
     1141 gctgcaaagt aatcccttcc cgcttaaaat atgtcacttg gactgtactg tattgtatcc
     1201 cagacgcccc tgcactaacg attcgagcca ggcgggaaag accttgtcct gatccacctc
     1261 ccatccaaat gtcacggagt ctcccagggg aggacccgat attcctccgc ggccttgggc
     1321 aagataggcc gattgcgcaa tccccgctcg ttatagctca cccgcacgtt gccgtagctg
     1381 gtcccccaca acggtttgtg ccggttgacg agctggtcat cggctacttt gtcccttatg
     1441 tagtctccta accgctcata gtaggagatc ccgagcagat cgatcggacg aaccgccagc
     1501 tcgagcgcac caagtgacag gatgaaggag acgccgctaa tgaccagacc agttcgggcc
     1561 tgatagatgg gctgatacca cgtcgatctc gccagcgaga gcgtcagggc aacaacaacg
     1621 aggaggccca gcagcacgat atacccctaa ctatactgcc cgaacaccac cagggtgtgc
     1681 gatcgatagg tagccgccag caaaacaccg ctccatgcca caacagcgac gcctattttg
     1741 aggcacctaa gatgagcgct cacagttctc gactcctggt gcgagaagag aatagaggga
     1801 ttgctgctca agactccttt tacaaacagc tctttgcgga aacttggttc cgtaggattt
     1861 cgaaatgttc cagacgtctc aacctctcac taccttctac caggatttct cataactcat
     1921 tccgtatgac actagacaag aattctgtct gctcgtccat agggtcatcc cttagcaaat
     1981 tgcaaaaaag acctgaatct aaactaaggg cacatgaaag gcttcacttg gagcgtacaa
     2041 gacggaaggc tcgttttagg aagcgttggt ttgaaattca ctgttgtgta ttgagataca
     2101 atatctaagg ttcctaagtt tcataaacgc attaatattg gacccgaata aaaccctaaa
     2161 tttcgacgcc cttcaataat acaaaagagg gggtgcattc ccttcggaag cctagcagtc
     2221 cggtattgat gtgaggcaac gtcggctact tccttttctt ctaatgcgtt atcggcgaag
     2281 caaatgggaa gagctcacaa cgttatttcg cctggcagac aagacaacag gcatggtcgt
     2341 cttcaggatg atgaagggag gattgcgtgg cgagagtcga caatccccat atcggactag
     2401 atattcgtgt atcgcagtgg aagtggattc agcatagact tcccggtttc accctgatcg
     2461 gtcccccctg gttgcagagt gagacattgg atggccgcgg aacaatcggc ttaagctgat
     2521 tcccctactt cgaggtctga gggcttgttt tctgatgcgg gaatgccttc ggggatcggc
     2581 tcccagtaca agatccgtcg gcaataggga cagagatgta aatcttccga ccgtttgact
     2641 tgcgcgataa gttgcggcgg gatctgtaaa cggcagccga gacagatgcc gtcacggact
     2701 gccgcaagag gatggtcttt tcgcgaggcc ttgacctggt tgtaccgatc aagcagggcc
     2761 ttctcaaccc tcactgaagc ctctcgatgg cggagttgga gttgagccag ttccgtcgca
     2821 tgctccttat cctgtgcatc tagtccctgc ttctcctgtg taaacacgtt ctcaagcgcg
     2881 gcttttttct cctgtagctc tttggcggcg ttctggagct gatcgatttt ctccatcgtc
     2941 aacagaatct tttcttcgaa gtctcctcgt ttcttattcg ccaactccag ctcaaacaaa
     3001 tgcgcctgat actccttatt ggtcttcagg cttgccgcgt gcgatttcat cttgtctgtg
     3061 tgtgcctcat gtgcctccag atccttttcg tgcgagcgtc gttctttaac ggcagcctcg
     3121 acggcgacct tcgtatcatt tagaattcga ctagcctctc gaagaggagc ctcggcagca
     3181 tggagacgtt ctgggatttt tcggcggatt tcactgatct ccatgattcg gagatcgagc
     3241 ttttgcagtt caatgagcgg ggaaagcttt tggttcaacg tatcccttcc atccatgtcg
     3301 gcacactgaa gtggcgggca accgtccgga ctggtgggcc cactaggact tgaacctagg
     3361 accagctgat tatgagtcag ccgctctaac cacctgagct atgggcccct tcagaaacgt
     3421 caccactcag gctgaggtcg agcatggaaa ccggatttta cagctttgat caaaacttca
     3481 tccttaatct caacctcggc ctgagacgga ttctaggacg ctgctccgag cagagtcaaa
     3541 tcacgccgtc ttgcactctg aaatctacaa gctctcgaca aaactcctca gctttttact
     3601 gcggctcgga tgccgcaatt tcctcaaggc cttggcttcg atttgcctga tgcgttcgcg
     3661 ggtgacttca aagtcctgtc caacttcttc caaggtgtga tcggtcgctt caccgatacc
     3721 aaaccgcttg cgcaacacct tttcctcacg aggcgtcaag gtttccaatg cgctgttgat
     3781 ctgccgctgc aagtcataac gaatggccgc ttccaacgga gacacggcct tcttgtcttc
     3841 gatgaaatcg cccagatggc tgtcttcttc ttcaccgatc ggcgtttcga gcgagatggg
     3901 ctcgcgggca atcttgagaa ttttccggac tttgtccaac ggcagatcca tgcgctcggc
     3961 gatctcttcg ggaagcggct cacgacccag cttctgcacg agatgcctgg aagttcgaat
     4021 gagcttattg atcgtttcga tcatgtgaac gggaatccgg atcgttcgtg cttgatctgc
     4081 aatagctctg gtgatcgcct gccgaatcca ccaagtggca taggtactga acttgtatcc
     4141 gcgcttatac tcgaacttgt ctacagcctt catcagtccg atgttacctt cctgaatcaa
     4201 atcgaggaac tggagacctc ggttcgtgta tttctttgcg atactgacca cgagccgcaa
     4261 attcgcctcg accaactcag ccttcccacg cttcaccttc tcttcggcta gatcgagatg
     4321 cttgactgca tccttgatct cttcagccgg aacaagtgcc tcttccgttt ccaactggcg
     4381 aatcttcgct ttggcggttt gatagacctt ccgaatctcc accagagctt cctccgacac
     4441 cccggtcttt cgtttgaccg ccaagaagtc ctgacggctc cggcacattc tcctcagcag
     4501 ttcggcacct acctcgcctc ccacaccgat ccgtcgttgg cagctgaccg cttcccgttc
     4561 ggagacacgg atctgtaccg ccagatcgcg gacacgctgg accattcggt ctttcagcac
     4621 accatggagg ttgaccgatt caattttgtt cacaacttgc tggcggatcg catcgaactg
     4681 cttcttgaac ttcttttgtt tggccggatc actgccgagg tgcttgccct tctcggccat
     4741 cgccttcagc gcgagcgaga ccttccgtac ggcattcaac gactcaagcg tcttgacccg
     4801 caactcctcg taatcccgct ccaccggtgg ctggtcctct tcaaagtcct cttcggtctc
     4861 ctggatcggc acaatctcgc gcacgtcgat cttggcgttc ttgagctgat cccggagagc
     4921 caagacgaat tcgatggtca tcggcatccc atagatcact gaggcgatat cattcttccc
     4981 ctcttcaatt cgctttgcga tttcgatctc gccttcgcgg ctgaggagag ccacactccc
     5041 catttccttg agatacagcc gtacaggatc gtcggtacgg ctgagagcgc caggcgtcag
     5101 atcgatcgcc ttatcgtttt cctcctcgga atccgactcg acgtcttcca catcttcgcc
     5161 gacttcgcct tcggatcgtt tccgaggtcg atccccatca ctcgcctcga tgatttcgat
     5221 atccatttca ccgaacatcg ccataatgct gccaaactga tctgaagaca caacttcagc
     5281 gggcaaggtg ctgttgagct cgtcatacgt cagaaagcct ttttccttcc cgattgtgat
     5341 cagcttcttc acctcgccga gcaactcttg tttcgacatg actactcctt caccaatgaa
     5401 gccactccgg aggtcggcgt ggcggctttc cgcatccgca cctcattgat ctgcatattc
     5461 agcaggtgcg cctcgtccat gcgaccttcc cgctcagccg ttttcagccg ggcaatgagc
     5521 tctctcatga cctgctccga tcgctttcgg tccaaacaat ccaaacaggc gacgatgtgc
     5581 gcttgcacat cgtcgaaatg atcctcacgc atcgacaatt ccgtcgccag agcaccacag
     5641 tcgggatcat ccaccgattc atccaaaact gatctgagac tgatacaccc ctctcgatcg
     5701 acatgggcga gtgcaatctc gacaagcttg cggcatggcg cgacggtaaa ggattccggt
     5761 cgcaggcgac tgacgtcggc aggcgacaat tttccgtgca gcagcagtaa gaggagatcc
     5821 cgctcttccg ggatcccttt gaaaagggca ttgaacgaga ttccctgtgt ggcctgcgcg
     5881 cgcggccctt cgccgcctcg tttcggctga gccagaagcg cgggatagcg ttcgatcagc
     5941 agggtctcgc tgattcccag ccgttccgac acgattctga tgcgctcctg ccgctcgatc
     6001 ggatgctcgc ttttctgcag aattcgcagc acctcatcga cgcttcggat gcgactttca
     6061 agtgagccgt cgtcggcctg cttgaccgtg tgattcagtg cgtagtccaa gagactcgga
     6121 gcagccgctt ccagctgggc aaacgccacg gcacctttct tccgcacaaa cgtatcagga
     6181 tcctcgtcaa ccggaagagt tacgactttg acgcccaatc cgctgttgac gaaaagatcc
     6241 aatcctctca atgccgcgcg cacaccggcc gcatcagggt caaagagcaa cacgacctgg
     6301 tcggcaaacc tccgcaagac ctgaacatgt tcagccgtca gggccgtccc cagtgtggcg
     6361 acggtatggg taagccccgc ctggtggagc gcgatcgcgt caaaatagcc ttcgacgatg
     6421 atcaccgtct tggtccgcac gatcgcctcg cgtgcctgat cgaaggcgaa aagggtctgg
     6481 ccctttttaa acaacaatgt atctggtgaa ttgagatatt taggcatgcg gtcgcccaac
     6541 acacgtccgc cgaatcccac cacgcgcttg cgcagatcgg tgatcgtaaa catgagccgt
     6601 ccatggaact tgtcatagta tccattaccg tttggtcttg ggcttccgag cccagccgtc
     6661 ataatctcgc tgtgagagaa tccttttcga gaaaggaatt taaacaaccc atcccactct
     6721 ggggacgaca ctccaatttg gaatgaatcc actgtcgatt gttggatacc tcgtccgttc
     6781 agatactcac gagccgtagc gccgatttgc ggatcccgca gattggcttg gaaccaggcc
     6841 atcgcgacgc ggttgatttc ttcaaggcga tgggtctgcc cacggtgcac tcgttccgca
     6901 agcgactcct ccaccataat gcccaccttg tctcccagtt ccttcaacac ttcaggaaag
     6961 gtcgcccccg taattttggt cagaaaggcg aagacatcgc ctccggcctt acagccgaag
     7021 cagtaaaaca tttgtttgga aggactgacc gaaaaagagg ggctcttctc ctgatgaaat
     7081 gggcacaacc ccacaaggtt ctgaccggcc ctcctcagtg agacatggtg tccgaccaca
     7141 tctgcgatat ctacacgatc tcttattcga ttcttgatat cgtcggaaat caagcttcgg
     7201 cccaccggag tcgcagctcc caccttcccg tcgtgcacga ccggacgagt cggttgataa
     7261 gtaatcggaa taattgaccg gtcagattaa cagacgagtt gaaaacctgt caattctgag
     7321 aattctatca cccgggaggc caagccatat gccgccctcc aagcacatga aaatggagat
     7381 gggaaacggt ttgtcctgca tcgcgaccgg tattcgcaac caaccgatag ccggactccc
     7441 gtactccttt caaggctgcc accttcgtac agctgatcag caatcgtgca agtaatgaat
     7501 catcccccat tcctaaatct tgcacggccg aaacatgccg cttcgggatc accaacgtat
     7561 gaacgggggc ttgaggattg atatcgtcaa aagccaatgt gtgctcatcc tcttgaacca
     7621 gcttcgccga gatcttcttc tccacgattt gacaaaaaag gcactcgctc atctcaacct
     7681 ctacaacaat ttaacggcat cgatccgcct cagaatctct atgaccatat tgggttactg
     7741 ccctgacctt aggctcggtt ttccgaatct gccccctaat tcttcataaa tatcctgcaa
     7801 aggcaggtca tgatacccca gcaccatgag ggtgtggaaa aacaaatcgg ctacttcatg
     7861 cacgatttct tctttcttgc cacctttgga cgccaacagg acttctccgg cctcctccgc
     7921 gacctttttc agaatcttgt catgcccctc ctcgaacaac tttgaggtat aggaaccggg
     7981 ctgtgggctg gagcggcgct cccgaattat ccgcaggaca ctttcaagga tccccccctg
     8041 cgcgtcatac gcactccgat gaacgacatg gccttgttca tccattttag agaagaagca
     8101 agcccgttcg cctgtatgac aggtcggccc ggctggttgc gccttcacca gaattgtgtc
     8161 gcagtcgcag tcaagaaaga gttccttcac atgtagcgta tgaccggacg tttcgccttt
     8221 ttcccagagc ttgttccgag atcgactcca aaagtgcact ctcttggtcg agaccgtctt
     8281 cgcgaaagcc tcttggttca tataccccaa catcagcacc gtcccatcaa gccaatcctg
     8341 aatgacgacc gggagaagcc cctcgccgtc gaacttgaac tgatctgtcg cttcctgact
     8401 catcgtgtca tgccactcga ggaagagtat cggatcggac agggacacca cgttcgcgca
     8461 aatacgcttt cgcttgggaa atcgtatagg tctgaaaatg aaaaatcgaa gcggccaaga
     8521 ctgcgtctgc tttccctttc acgaaaccat cgtataggtg gtcgagcgcc ccgactccac
     8581 ccgatgcaat cacgggaatc gacacagagc ctgacacggc agctgtcaga ttcaagtcat
     8641 acccggtctg ccgaccatcc tgatccatac ttgtgagtaa aatctctccg gcgccgtact
     8701 cttccatccg tttcgcccac tcaaccgcat caagtcccgt cgcatggcga ccgccgtgcg
     8761 tgaagacctc ccagcgatca cccgttgcgc gcttggcatc gatggcaacg acgatgcatt
     8821 gcgtcccgaa acgccgagcg gcctctttca cgaactcgga ccgtcgtact gccgctgtat
     8881 tgatgctgac cttatctgct ccggcattca acaaggcccg aatgtcgtcg agcgtcccca
     8941 cgccgccacc gactgtcacc ggcatgaata ccctggcagc cgtccgctca acgacgtcga
     9001 tgatcgtctt ccggttttca tgcgaagccg tgatatcgag aaaacagagc tcgtccgcac
     9061 cttcgtgatc ataccccact gcggcttcaa ccggatcccc ggcatctcgg agattcacaa
     9121 agctgacgcc tttgaccacg cggccctctt tgacgtccag acagggaatg atgcgcttgg
     9181 tcaacatgtg gtgtcgtcac agggaaagcg ccgccacagc ggcttgataa tccagttcgc
     9241 cgtcatagag tgctttgccg ataatcgctc cctcgatctt agggccgatc gattgcactg
     9301 ctcgcagatc ctccagggac gtgatgcctc ccgacgctat caccgggaac gaagagaatt
     9361 cggtgatttc tatcaacgcg gggaggttcg gtccattcag catgccatcg cgtgcgatgt
     9421 ctgtatacac tacggcggca atcgcgcaac ctgaaagttc cttcaacaaa tcgatcgctt
     9481 tcacatcgga taccgttgtc cagcctttta ccgctattcg gccctcacgt gcgtcgagac
     9541 caagtacgat ccgctgcgga aactcccggc aagcaagatt gagaagcgtc cggttcgtga
     9601 gtgccgccgt gccaagcacg acacgtgaca ccccggcatt gagataccga cgaaccgtat
     9661 ccatggtcct gataccgcca ccgacctgaa tttttacgtg aaccgctttt ataatggact
     9721 cgatttgggg caggttcttg ggctcgccgt caaccgcgcc gttcaaatcc acgacgtgaa
     9781 tcagaccggc tcccctttgt tgccaccttt cggcaacttc cgtgacatcg tcggaataga
     9841 ttgtttcagc cgccatatcg ccttgacgca gccgtacaca gcatccatcc ttcaagtcga
     9901 tcgcgggaat aatcaacatg ctatcgttcc tctcccctgc cgaacggaaa ttccttcaac
     9961 acctcgtcca ccccatactc tgccaattcc gccgccgtca cgaaagccca tcggtgtaca
    10021 aatcccatga tatc
//
LOCUS       DATNDD010000395         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4200983, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000395 DATNDD010000000
VERSION     DATNDD010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4200983"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VN647_09615"
     CDS             complement(<1..333)
                     /locus_tag="VN647_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HWG97328.1"
                     /translation="MRMTAGRRALDKIYRRRDRYEIPEWQRGEVWDTERKQQLIDSIL
                     RGWKLPKFYFLRLDDDDFEVVDGQQRLAAIYEFFSNELPLAEESAKEFGGPYYKDLKQ
                     RIADTFDDF"
     gene            794..1585
                     /locus_tag="VN647_09620"
     CDS             794..1585
                     /locus_tag="VN647_09620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG97329.1"
                     /translation="MVSKLTDRHELKRFDCIWTGFNIRTRKCYDDVVMEDRFAMATDA
                     GGKGTEAGYDCWAATYDDGDPSTWLDEPFLLDQLKPFPGCRILDLGCGTGRYLRRLAS
                     GLYRITAVDLSRGMLCRARRDIERREEISWVQTSVTCLPFQSRLFDRVMSGLVLDHIE
                     RPEHLFGQIAAVLKQKGRAVVTAVHPDMQRLTGADIELASLNGSVRISGHLHEVRSLI
                     VAAQDATLMVECIEEPVVTEAMVSRRSDWRHKLGCPALLLLALSK"
     gene            complement(1776..2384)
                     /locus_tag="VN647_09625"
     CDS             complement(1776..2384)
                     /locus_tag="VN647_09625"
                     /inference="COORDINATES: protein motif:HMM:NF015858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HWG97330.1"
                     /translation="MELRRLIFLLLTSLTFGGCAANVNVNYVPDPDHKSPLEILKHLT
                     VSVAAEDQRNETERNRVGDRKNGFGQPVAPVILTNQSPMVLVQEALRKEFSNNGIQLV
                     DATSNVPHKAITAKLKKYWVENKMNFFDITMYSTISADILVEDIPPSGRTSTHTITGT
                     ARDSRQLAFESAYEETLNEAMAEFIQSFSRDPGLLSSLRGSP"
     gene            complement(2397..>2633)
                     /locus_tag="VN647_09630"
     CDS             complement(2397..>2633)
                     /locus_tag="VN647_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97331.1"
                     /translation="RSCVGESSPEHMQVEWHIDEKLIHRKEGRTIWERSEVYRDPICR
                     PFPEFRDQPTLLRERLHQSLETVAGVLINRILYP"
BASE COUNT          621 a          668 c          698 g          646 t
ORIGIN      
        1 gaaatcgtca aaggtatcag cgattcgctg cttcagatct ttgtaataag gtcccccaaa
       61 ctctttagcg gattcctcag ccagcgggag ttcgttagag aagaattcgt atatcgcggc
      121 aagcctctgc tggccgtcaa ccacctcaaa gtcatcatca tccaatctaa gaaagtagaa
      181 ttttggtaat ttccagcccc ttaggatact gtcgatgagt tgctgctttc gttcggtgtc
      241 ccagacctcc ccccgttgcc actcaggaat ttcataacga tctcgacgcc tatagatttt
      301 gtctaatgcg cgtcggccag cagtcattct catacgttgt tatcctgatg cgggcagttg
      361 agactctaat gatcgaggca agcataagaa atgtaagcat tgtctttaga gatgtcaata
      421 acctctctga cgagcatatc caggacggct gtatccccca taatgtatct ccgctgctac
      481 aaggaagatg ttaaagccca acgagcccac cgaattttac ggcttgtttc gaatcattca
      541 ccagcaagcc atagagtcga cgttccgctt cggcatctgt acacggagca aggaggaagg
      601 atggatggtc ggcagaatca tgccttatgg agtggttcta ggtggcccca ctccttcagc
      661 actgggagag tatagcccaa cggtgattgc gcggcggtgg cgccgaggca gacgatgacg
      721 ctcggccgca ttggcgccag ttccgactcc aatcatgggc gataagccgt cactacggaa
      781 cacacggtcg ttgatggtgt cgaaacttac cgatcggcac gagctgaaga ggtttgactg
      841 catttggacc ggattcaata tcagaaccag gaaatgctac gatgatgtgg tgatggaaga
      901 ccgcttcgcc atggcgactg atgcaggggg aaagggaaca gaggcaggct acgattgctg
      961 ggccgcgacg tatgacgacg gcgatccctc tacctggctg gatgagcctt ttctcttgga
     1021 tcaactcaaa ccgttccccg gctgccgaat cttggactta ggctgcggaa ctgggcggta
     1081 cctccgccga ttagcgtcgg gcttgtatcg catcacggca gtggatctct cccgcggcat
     1141 gctctgccga gcgcgtcggg acatcgaaag gcgagaggag atttcgtggg tacagacatc
     1201 ggtaacttgc ttgccctttc agtcccgtct ttttgatcgg gtcatgtccg gtctggtgct
     1261 cgatcacatc gaacgaccgg aacatctatt cggacagatc gccgctgtat tgaagcagaa
     1321 aggtcgagcg gtcgtgacgg cggtacatcc ggacatgcag cgtctcaccg gagccgacat
     1381 tgaattggca agcctcaacg ggtctgtacg aatctcaggg caccttcacg aagtgaggag
     1441 cctgatcgta gcggcgcaag acgcgacgct gatggttgaa tgtatcgagg aacctgtcgt
     1501 gaccgaggca atggtgagcc ggcgttccga ctggcggcat aagcttggct gtcctgcctt
     1561 gctgctcctg gccctcagta aataagcagg ggatcgggtc acgaataggc tctggcaaaa
     1621 tccgtagctt gtcattggta gcaccggcgg ccatacatcg gttcaattca actagcgatt
     1681 gctattcatg ctgatactta acgatcgtta gtccgcctag gctaagaaga acacattcga
     1741 atacagtgga aaaatgacga agcccattta tggagttagg gtgagcctct gagcgaggag
     1801 agtagtccgg ggtccctgga aaaagactga ataaattctg ccattgcctc attcaaagtt
     1861 tcttcataag cggattcaaa tgccagttgg cggctatccc gagctgttcc cgtaatcgtg
     1921 tgtgtcgatg tgcgaccact cggcggaata tcttctacca agatatcggc acttatcgtc
     1981 gaatacatag tgatgtcaaa gaaattcatc ttgttttcga cccagtactt tttgagcttg
     2041 gcagtgatcg ctttatgcgg aacattactt gtggcgtcta caagctgaat gccattgtta
     2101 ctaaactcct tccgcaatgc ttcttggact agtaccatgg gcgactgatt cgttagaatt
     2161 acaggcgcga caggctgtcc aaatccattc tttcggtcgc ctacacggtt acgttctgtc
     2221 tcgttccgct gatcttccgc cgctacggat acggtcagat gtttgaggat ttccaaaggg
     2281 cttttgtgat cagggtctgg aacgtagttc acgttcacgt tcgccgcaca tccgccgaaa
     2341 gtcagactgg ttagcaggag gaagatgagt cgacgtaatt ccatatcatt tctccgttat
     2401 ggatagagaa tacgattaat gaggactcca gccacagtct caagcgactg atgaaggcgt
     2461 tccctcagca aggtcggttg atctcgaaat tcagggaacg gacgacagat tggatcgcga
     2521 tacacctcgc tccgctccca gatggtccga ccctccttgc gatgtatcag cttttcatca
     2581 atatgccatt caacttgcat gtgctccggg gacgactctc caacacagga acg
//
LOCUS       DATNDD010000396         5421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3910313, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000396 DATNDD010000000
VERSION     DATNDD010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3910313"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..2451
                     /locus_tag="VN647_09635"
     CDS             100..2451
                     /locus_tag="VN647_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HWG97332.1"
                     /translation="MSMIETMEPVVPDPPGEPLSVQIREYWGNIRRRKWLVLGSIVAG
                     TVVAVTLCMVLPKSYRSNTLILIEDQKIPEDYVKGIGGASVEQRLTMIQQQVMSRTIL
                     SQILGEYDLYERQVEREGLESAIETLRKMIKVETVGTLGASGKSVEAVTLSFAHEDPT
                     TAMKVTAKLAALFIEENVKAREQLVTGVSVFLEQELFEAKKALEAQEEAISKYKTKYV
                     GQLPEQMEANLRALDRLQTELNTTDDLLHSQTDRLSTVEKSIKEYQATGGTGTDSDDR
                     KSMHTAMDPLVVRLHELERRLTSLRAEWKETYPDIADTKQEIEDVKAQLTEKYSAQTT
                     DKDGESSETHDPYLRELLKQRNDLRTDLSAIRDRRVRLVEQIRDTERRVERTPSREQE
                     LMILVRDYENRKKNYEALLDKRLNAHVAENLEKRQQGEQFRVLDPANLPQKPEKPNRI
                     VIMVFGLIGGCGLGVGLAIGLDLLNPTFRRREEVEIMPGIQVLATIPQFFTLSPQISA
                     ISRTGSQMADDGSSGVPAVTIAAKVMPWASSGNRSEFNPTNLNLVAKWQPRSIAAEQY
                     RMAATKLVLSTEGRRSTVVEITSALMGEGKTTTVVNLGYTIARDFGKKTLLIDCDFQR
                     PALHHYARTPARAGLIEFLDGRASLDDCVSAIDEYPCSVMAAGGAVGTGGEWNELGRI
                     QQLKAMLPELRAKFQYLIINTPPVLASATMGILASLADELVLVIRAGHSPKHLVQKAF
                     TMLGLTAQRRVILNGLDAQSMPHHLYGYTVPYGLDRSVESMSR"
     gene            2589..3200
                     /locus_tag="VN647_09640"
     CDS             2589..3200
                     /locus_tag="VN647_09640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWG97333.1"
                     /translation="MEHTTERANGHTDPVTQHDSKPALHLPLRIVESDMSTAPINGYL
                     CIAPDVKVGSNVKFSKFVNLYGCEVGDDTKIGAFVEIQKNAKVGKRCKISSHTFICEG
                     VTIEDEVFIGHNVTFVNDSFPRATTANGSLQTEADWLVETTLVKRGASIGSGATILAN
                     VVIGEHALIGAGAVVTKDVPAHAVVAGNPGRVVRYVESGGSHD"
     gene            3193..4395
                     /locus_tag="VN647_09645"
     CDS             3193..4395
                     /locus_tag="VN647_09645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWG97334.1"
                     /translation="MTKENIPFLDLVTVHQELQGELVEVLKTALKSAGFIGGPMVQGF
                     EEDFSRFCETRFCVGVGNGTDALRFALIAAGVKSGDIVLTVPNTFIATTEAISQAGAQ
                     PDFVDIDPQTYAMEPGKLREYLEHKCKWNTKTRQVIHKATGKPITALVPVHLYGQMAD
                     MDPILDICAHYNLKAIEDACQAQGAEYFSRKDNRWYKAGSMGHAAAFSFYPGKNLGAC
                     GEAGAVTTSDDGIAQTCRLLRDHGQSKKYFHDIEGYNGRLDAIQAGFLRVKLHHLPKW
                     NEQRRQAAERYDKLFAGAEGIVTLPHQPNWARSVYHLYVIQVEDRILLQQKLSDVGIG
                     TGIHYPVTLHLTKAYEGLGFHHGDFPIAEKSAAHILSLPMFPDLSPVQQERVVTEVLK
                     SLSVNNES"
     gene            4430..>5421
                     /locus_tag="VN647_09650"
     CDS             4430..>5421
                     /locus_tag="VN647_09650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG97335.1"
                     /translation="METRSCLFCGVPLRRTFVDLGMHPLCESYVSEAKLNAMEPFYPL
                     HVYVCEQCFLVQLHEYVSPADIFTEYAYFSSYADSWVQHAKRYTEMIEERLKLTAKSL
                     VVELASNDGYLLQHFVAKGIPVLGVEPAANVAEVAKKKNVPTLVKFFSRNTANELVAE
                     RGTADLVLGNNVLAQVPDINDFVEGIKILLASRGTVTIEFPHLMRLMQENQFDTIYHE
                     HFFYFSLMSAERIFAAHGLTLFDVEELPTHGGSLRIYGRHADDETQPVTERYRELMRR
                     EKAAGFDRPETYALFGEQVKETKRKLLEFLIEAKRKGKTVAAYGAPGKGNTLLN"
BASE COUNT         1337 a         1346 c         1578 g         1160 t
ORIGIN      
        1 gctgtcctat acccatagcc gattcgaccg gaccgtagct tccgagagat tcccttttga
       61 ccggaatctc gtccaactca gcttggtcgc ggagtggaaa tgagcatgat cgaaaccatg
      121 gagcccgttg tgcccgatcc accgggagag ccgttgtcgg tgcaaattcg agagtattgg
      181 ggcaacatcc gccgtcgaaa atggctcgtg ctcggctcta ttgtagctgg gaccgtcgtc
      241 gcggtaacgc tctgcatggt tctgccgaag agctatcgct ccaataccct gatcttgatc
      301 gaggatcaga agattcctga ggattacgtc aaaggcatcg gaggggccag cgtggaacag
      361 cgcctcacga tgattcagca acaagtgatg agcaggacga ttctgagtca gattttgggc
      421 gagtacgacc tctatgagcg acaggtcgag cgggagggcc tcgagtcggc cattgagaca
      481 ttgcgcaaaa tgatcaaagt ggagactgtt ggtaccctcg gcgcatcggg caagagtgtc
      541 gaggcggtca ctctttcatt tgcccatgaa gatccgacaa cggccatgaa ggtaacggcg
      601 aagctcgctg cgctatttat cgaggaaaat gtcaaagcac gggagcagct cgtgaccggg
      661 gtgtccgtct ttctcgaaca agaattgttc gaggcgaaga aagccttgga ggcacaagaa
      721 gaggccatca gcaagtataa gacaaaatat gtgggccagt tgccggagca aatggaagcg
      781 aatctcaggg ccttggatcg gctgcaaacg gaactgaaca cgacggacga cctgctgcat
      841 agtcaaaccg atcggttgag cacggtcgaa aagtcgatca aggaatatca agctaccgga
      901 gggaccggta cagattccga cgatcgtaaa tctatgcata cggcgatgga tccacttgtg
      961 gtccgattgc atgaattgga gcgacgtctg acctcgctcc gggccgagtg gaaagagacc
     1021 tacccagaca tcgccgacac gaaacaggag attgaagacg tcaaggctca gttgactgag
     1081 aaatacagcg cacagacgac agacaaagac ggcgagagtt ctgaaaccca tgatccctat
     1141 ttaagagagc tgttgaagca gcgaaacgac ctgcgcaccg atctgtcggc gatcagagat
     1201 cgtcgagtca gactcgtaga acaaatcagg gatacggaac ggcgtgtaga acggacaccg
     1261 tcgcgagaac aggagttgat gattttagtg cgcgactacg aaaacagaaa aaagaactac
     1321 gaagcattat tggataaacg attgaacgcc cacgtggcgg aaaaccttga gaagcggcaa
     1381 caaggggaac agttccgtgt gctggacccg gcgaatctgc cgcagaagcc ggaaaaaccg
     1441 aatcgcatag tgatcatggt ttttggcctg ataggaggat gcggactcgg tgtcggtctt
     1501 gcaatcggct tggatctgtt gaatccgaca tttcgacgtc gagaagaggt ggaaatcatg
     1561 ccgggcatcc aggtgctcgc gaccataccc cagttcttca ccttgagccc tcagatcagc
     1621 gcaatatcga ggaccggctc tcagatggcc gatgacggta gttcaggggt gccggcggta
     1681 acaatagcag cgaaggtcat gccttgggcg tcatcaggaa ataggagcga gttcaatcca
     1741 acaaacctga atctggtcgc caagtggcaa cctcgttcga ttgccgccga acaatatcgg
     1801 atggctgcca cgaaactggt cctctcaacc gagggacgcc gttccacggt cgtcgaaatc
     1861 acaagcgcat tgatgggcga aggtaagacg accacggtgg taaatctagg ctataccatt
     1921 gcgcgagatt tcggaaaaaa gaccctgctc atcgactgcg acttccaacg gccggccctc
     1981 catcactacg cccggacacc tgctcgagcg gggctgatcg agttcttgga tggtcgggcc
     2041 tcactcgatg actgtgtgtc ggcgatcgat gagtacccat gctctgtcat ggcggccggc
     2101 ggagcggttg gaacgggtgg cgaatggaat gaacttggga ggattcaaca gctaaaagcg
     2161 atgctccctg agcttcgcgc gaaatttcag tatctcatca tcaacacgcc acccgtcttg
     2221 gccagcgcga ccatgggcat cttggcgagc ttggcggatg agcttgtgtt ggtgatccgc
     2281 gccggacatt cgcccaagca tttggtgcaa aaggccttta cgatgctggg gctgacagcg
     2341 caacggcgag tgattctcaa cgggctcgat gcgcagagta tgccgcacca tttgtacggt
     2401 tatactgtac cctacggtct ggaccgttcg gtcgagagta tgagtcggta ggcagaagag
     2461 tcttcctcgc agctcacgtc gccaacttat cgtccgcaat agctttcact cgctattccg
     2521 acatcagctg cgcccgttcg caatgcgttg accttatagc ggctgatctc atcattttgg
     2581 agaaatcgat ggaacacacg acggagcgag ccaacggaca tacggacccg gtcacccagc
     2641 atgacagtaa accggctctc catcttccgc ttcgcatcgt ggagtcggac atgagtacgg
     2701 cgccgatcaa cgggtatctc tgtattgcgc ctgatgtcaa ggtcgggagc aatgtgaaat
     2761 tttccaagtt cgtcaacctc tatggatgtg aagtgggcga cgacaccaaa atcggggcgt
     2821 ttgttgagat acagaagaat gccaaggtcg gcaaacggtg caaaatctcg agtcatacct
     2881 ttatctgcga gggcgtgacg atcgaagacg aagtctttat cggacataac gtcacgtttg
     2941 tgaacgattc gtttccccgt gcaacaaccg ctaacggctc tctccagacg gaggctgatt
     3001 ggttggttga aacaacattg gtgaagcgtg gcgcatcgat cgggtccggg gcaacgattc
     3061 ttgcgaacgt ggtgatagga gaacacgcgc tgatcggagc cggcgcggtc gtgacgaaag
     3121 atgtgcctgc gcatgccgtc gttgcgggaa atccaggcag ggtggtgcgg tatgtcgagt
     3181 cgggaggcag ccatgactaa agagaacatc ccctttctgg atctcgtcac ggtgcatcag
     3241 gagttgcaag gtgaattggt cgaagtgctg aagaccgccc tgaagagcgc cggatttatc
     3301 ggcgggccga tggtgcaagg attcgaagag gacttttctc ggttctgtga gactcgattc
     3361 tgtgtcgggg tcggcaacgg tacagatgcc cttcggtttg cattaattgc cgccggtgtg
     3421 aagtccgggg atatcgttct cacggttcct aatacattca ttgccacgac tgaggcgatc
     3481 tctcaggccg gcgctcaacc ggatttcgtc gacatcgatc cacaaacgta tgcgatggag
     3541 cctgggaaat tgcgtgagta cctggagcac aaatgtaagt ggaatacgaa aacacgacaa
     3601 gtcattcaca aggcaaccgg taagccgatt accgccctcg ttcccgtcca tctctatggg
     3661 cagatggccg acatggatcc gattttggat atttgcgccc attataacct aaaggccatt
     3721 gaagacgcct gtcaggcgca gggagctgaa tacttctcac ggaaggataa tcgttggtac
     3781 aaggccggct caatggggca tgccgctgcg ttcagttttt atcccggaaa aaatctcggc
     3841 gcctgcggcg aagcaggggc ggtcacgacc agcgacgacg ggattgcgca aacgtgcaga
     3901 ctcttgcgcg accatgggca atcaaagaaa tattttcacg atattgaagg ttacaacggc
     3961 cgtcttgatg ccattcaggc cggcttcttg cgtgtcaaac tacaccattt gccgaaatgg
     4021 aacgaacagc gtcgacaggc ggcggagcgt tacgacaaat tgtttgccgg agcagaaggc
     4081 atcgtgacgt tgccgcatca gccgaactgg gcgcggtcgg tttatcatct gtatgttata
     4141 caggttgaag accgtattct acttcagcag aagctgagcg atgtgggcat cgggacgggg
     4201 attcattatc cggtcacgct gcatctaacg aaggcctacg aaggactcgg cttccaccac
     4261 ggggatttcc cgatcgccga gaaatcggcg gcacatatcc tgtcgcttcc aatgttccct
     4321 gatctaagcc cagttcagca ggagagagtg gtgacggaag ttctgaagtc tctctctgtc
     4381 aacaacgagt cgtagatccg ggtgccgtct gcaaggaagg atcggctaca tggaaaccag
     4441 aagttgtttg ttctgcggcg tgccgttgcg aagaaccttc gtggaccttg gaatgcatcc
     4501 tctctgtgaa agttacgtga gcgaagctaa actgaacgca atggaaccct tttaccctct
     4561 ccacgtgtac gtctgcgagc agtgttttct cgtgcagctg cacgagtatg tcagtccggc
     4621 ggacattttc acagagtacg catacttttc ttcttacgcg gacagttggg tgcagcatgc
     4681 aaagcgatat acggaaatga tcgaagagcg gcttaagttg acggcgaaga gtttggtggt
     4741 cgaacttgcc agtaatgacg gctacctgtt gcagcatttt gtcgccaagg gcattccggt
     4801 cttgggagtc gagcctgcgg ccaacgtagc cgaagtggcg aagaaaaaga atgtccccac
     4861 gttagtgaag ttcttcagtc ggaatacggc caatgaactg gttgccgaga gagggacggc
     4921 cgaccttgta ttgggaaaca atgtgctggc ccaggttccc gacatcaatg actttgtcga
     4981 gggaatcaaa atcttgttgg cctctcgtgg cactgtgacc atcgagtttc ctcatctaat
     5041 gagattgatg caagaaaatc agttcgatac catctaccat gagcatttct tctacttttc
     5101 cctcatgagt gccgaacgga tctttgcggc tcatggactc acgctgttcg acgtggaaga
     5161 actgccgacg cacggagggt cgcttcggat ctatgggcgt catgccgatg acgaaacaca
     5221 gcctgtcacg gagcggtatc gagaattgat gaggcgagag aaggcggccg gcttcgatcg
     5281 accggaaacc tatgcgctct tcggtgaaca ggtgaaggag acgaagagaa agttactgga
     5341 attcttgatc gaagccaaac ggaagggaaa gactgtcgca gcgtacggag cgccggggaa
     5401 aggcaatacg ctcctcaact a
//
LOCUS       DATNDD010000397         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3060037, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000397 DATNDD010000000
VERSION     DATNDD010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060037"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VN647_09655"
     CDS             complement(<1..1355)
                     /locus_tag="VN647_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97336.1"
                     /translation="MGSRFPSLTVRRVLTNAARGFALAILISGIAHAESAPQEPTPME
                     EPTPLAGGDFRTKLFGEEIHVQPRDRRSVTAASFGLQWIPDGPAQLEVLPFGALYVWR
                     NWDDDNRRFRGTISAVVNDLTYSIGLRSYPNWTVLFTWNNFIAPLGRSENVEGQRIRA
                     TEIEWSYLFGGVGLGYRLPIHPFKQDSAVNLFLTYEPGFRWFKGTSHTSPQYGVPSDT
                     YEGRLHFKARIDALTRNLMELPHEGITLGGDVVYGHRLKWTAWGGPPFDTPDFKNDRT
                     YFAVSGYLMAASGLPFVESQKHRLVSSLHAGIGKDLDRFTAFRLPGRPTGYEWDAVSL
                     PMIHGVAFNELFPRHYAIANLQYRYEALFFLYPYVEAGLAFVDRPRFTADGRIKQSTD
                     SMPTVGVGVVSGAPWRSQIELNYTYNFGIFRDSDAGAPTRGGHGMFLFWSRELGATSR
                     GS"
     gene            complement(2348..>2530)
                     /locus_tag="VN647_09660"
     CDS             complement(2348..>2530)
                     /locus_tag="VN647_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97337.1"
                     /translation="KKLYEAVANGVLDLFKSPPTGQVATETDVSGPVDHPHASTWQIL
                     EKLVQNAFFKAILPGF"
BASE COUNT          582 a          723 c          680 g          545 t
ORIGIN      
        1 gagccccgtg aggtggcccc cagttccctc gaccagaaca ggaacatccc atgaccgcct
       61 cgtgtggggg ctccagcgtc cgaatcgcgg aagatgccaa aattatacgt atagttcaat
      121 tcaatttggg accgccacgg agccccggac accactccca cccccacggt aggcattgag
      181 tcggtcgact gtttaatccg gccgtctgcc gtaaagcgcg ggcgatcgac gaatgccaag
      241 ccggcttcca catagggata cagaaagaag agggcttcat accgatattg cagattcgca
      301 atggcgtagt gccggggaaa gagttcattg aacgccacgc cgtggatcat cggcaacgac
      361 acggcgtccc attcgtaacc ggtggggcgt ccaggaagcc ggaacgccgt aaatcggtcc
      421 agatccttcc cgatgcccgc gtgaaggctg gagaccaacc gatgtttctg actttcgaca
      481 aaaggcaacc cggaagcagc catcaggtat ccgctgactg cgaaataggt gcggtcgttt
      541 ttgaagtcgg gggtgtcaaa gggcggacct ccccacgccg tccacttgag gcgatggcca
      601 tagaccacat cgcctcccag tgtgatccct tcatgaggta attccatgag gttacgagtc
      661 agggcatcga tacgggcttt gaagtgcagc cgtccttcat aggtatcgct cggcacgcca
      721 tactggggcg acgtatggct ggttcccttg aaccaccgga accccggctc ataggtcaga
      781 aaaagattga cggcgctatc ttgtttaaaa ggatgaatag gaagacggta cccgagtccc
      841 accccaccga agaggtaact ccattcaatc tccgtggccc gaatccgttg cccttccaca
      901 ttctccgacc gtcccagcgg ggcgataaag ttgttccagg tgaagagcac ggtccagttg
      961 ggatacgacc ggagcccgat cgagtacgtc aggtcattga cgacagccga aatggtgcct
     1021 cgaaatcgtc ggttatcatc gtcccagttg cgccacacat acagtgcgcc aaagggcagc
     1081 acttcgagct gggcggggcc gtccggaatc cactgcaatc caaagctcgc ggcggtgacg
     1141 gagcggcgat ctctcggttg gacatggatc tcttctccaa acagcttggt acgaaagtct
     1201 cccccggcca gtggagtcgg ctcctccatc ggggtgggtt cctggggtgc cgactccgca
     1261 tgggcgatcc cggagatgag gatcgcaagc gcgaaccccc gggcggcatt ggtgaggaca
     1321 cgacggacag tcagggaggg gaatcgtgag cccatcacgc cccctcgcat tcagtcccaa
     1381 tgtctaccat gtcgcctcct ttttatccca aaccaggcgt gtctgagagg atcatcgtgg
     1441 gaaacacgag gctcacactc atgcttcctc tgccgcgcga gtttcgtacg acacattctc
     1501 actattgtca gcggatagtc tcggtgaccc ttttagttca tgtgacatat ttaaaaaaga
     1561 catgtatcac tgaattacat caatgtattt gatgtaatac ctgttcatca gtggtacgca
     1621 ttcagaaccg aggagtccat cctatacaat cctccatcaa attcttaccc gtgcccactc
     1681 cttcggatcg acaagctccc gaatctcata aggcacaccg attcgtttag gagtaacaca
     1741 aggctagcgg cgatctgacc caacagggca acaagcgatc gagttgagcc gattagtttt
     1801 acgttttacc tgccacccgt ttctgataca agacttccca gccagccttc ctatgctcag
     1861 gcaggccgaa cacttcctgg tacagatccc tgtacggaca ttgatacgtc agcagaactg
     1921 ctgtggcaca cgcctattct caatttcggt ccttctaggc tcatgaggag ctggtcgagc
     1981 attttctgct gacatcgatg gaggtgcacc tgatcctcag ctgccgcggg aataccaagc
     2041 ggtgggggat agtgttgttg ctgaagacgg tccggcatca cataacaaac atcttcgata
     2101 aggtcggtgt gccgaatcgg cagaaactcc tggtccatgg ccatcatctt cgggccatgc
     2161 caacctaacg atggagactg gacgagagtc gcgtccgcaa ggacgcaccg attggcacag
     2221 ccagccaaga acggcccctc gaaggcacgg ggttcctctg cttgtcgctt gaaccaaagt
     2281 tctcatagaa tacgttccac ttcaccgcag gattcaccta cacgtcatat caggcccttc
     2341 tctgctctta gaatccagga agaatggctt tgaaaaaggc attctgaacg agtttttcaa
     2401 gaatttgcca ggtgctggcg tgcggatggt cgaccggtcc tgacacatcc gtttcggtcg
     2461 cgacttgccc ggttggaggg ctcttgaaga ggtcgagcac gccattggcg acggcttcat
     2521 agagtttctt
//
LOCUS       DATNDD010000398         2936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_761487, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000398 DATNDD010000000
VERSION     DATNDD010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761487"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VN647_09665"
     CDS             complement(<1..655)
                     /locus_tag="VN647_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97338.1"
                     /translation="MLPSRRYLCLILPAYLFASGCVSTLPPAKSIHAFDRGCIPPEAR
                     FKSIPFSEVETVPSSTEAIKDGDAKKYYSPIAVDIADVMHVLPLLNRLAFLERAHEDA
                     NEAARVRQRLLNMVQLATLEVSSLVAELECEVHRADEVQDRLKDIQYSRSMSQTILAI
                     MIGGFIDAITGGFSIATRAINVISVMGGTLEILFGASANFTKVKQEFRHPHNHLERIW
                     "
     gene            962..1507
                     /locus_tag="VN647_09670"
     CDS             962..1507
                     /locus_tag="VN647_09670"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWG97339.1"
                     /translation="MKSALTVLITIIAGILIAGCSLVQRAMPGSTMSNAEIIGVLNAI
                     DHNEIDVGQLALQKAPTPEVRSFASRMVREHEMMMQNTDRLAHRLSVQPQNPALVSTM
                     ADTHREMMKKLGAKYGSDFDKAYIGYQIKMHEQALDLVKDVAESVENRSLKLHLREMR
                     PDLQNHLTSAQSIEQQVVAQQ"
     gene            1883..>2936
                     /locus_tag="VN647_09675"
     CDS             1883..>2936
                     /locus_tag="VN647_09675"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWG97340.1"
                     /translation="MKINRRSLLRFSLVWPATAPMWAGTLLQAAESKDDRRKETLTKE
                     RPAATDSLADAIVIKNENVFFVAQPDGNVPMRDSHGMGLYYRDCRYVNGYELALAGKQ
                     PAHLSASSAQGSIGVFTLTNPRIELVNGDILHEEKLGITWRRLMDHETPALRDCLLIQ
                     NFTLQALEFPVSLTIQSAFEDIFAVRGLLPKQFGTLHRPSWEAGTLLWVYDGKDGVVR
                     TLTVHFDPLPDKHQGTTAEFAVRLSPRGQQRIDVSLVIGESKNRTTARRMRPDPADFS
                     RSESELERETQVWMAHVTRVATNGSALDQLMERSFRGLRVLRTHLGDETFFAAGVPWF
                     VTLFGRDSLITAWQMLA"
BASE COUNT          656 a          816 c          813 g          651 t
ORIGIN      
        1 cccatattcg ttcaagatgg ttatgcgggt gcctgaattc ttgcttcacc tttgtaaaat
       61 tagccgatgc tccgaatagg atctccaacg ttcctcccat gacggaaatg acattgatgg
      121 cgcgcgtcgc gatgctgaat ccaccggtaa tggcgtcaat aaagccgccg atcatgatcg
      181 ccaagatggt ttgggacatg ctgcgactgt attgaatatc tttgagccga tcttgcactt
      241 catcggcgcg atggacctca cattccaact cggcgacgag gctcgacacc tcgagtgtcg
      301 caagttgaac catgttgagc agcctctgcc tgacgcgcgc cgcttcgttc gcgtcctcgt
      361 gagcacgttc gagaaatgcc agtcgattga gcaacgggag cacatgcatg acatcggcga
      421 tgtctactgc gatggggcta tagtactttt tggcgtcgcc gtctttgatt gcctcagtag
      481 aggaagggac cgtttcaacc tctgagaacg gtatgctctt gaagcgggcc tccggtggaa
      541 tacaccctcg atcgaaagca tggatagatt ttgccggcgg cagcgtagag acacagccgc
      601 tcgcgaacag gtaggccgga aggatcaagc agagatatct tcgactaggc aacacagttg
      661 gtcgttgctg acatgccgtg tctcctggga catttccgtt ccaatcagag aatatagtag
      721 cattctgtcc gattcagaga caatcgaccc tcaatacttc atcggtcttg tcagccgcct
      781 cttcatatcg ttcgcaacgc aacgtaatgt gatgaggtca ggctctcgca ctgcaaacga
      841 tctatgctgg cacatatcct gctgcctatt attatgaaga agaagcacct acgtttgaac
      901 agttcgagcc tacggatcgg aaatgaattt tcaacctaca agaaccaaca aggaggtctc
      961 catgaaatca gccttgactg tcctcatcac aatcatcgcc ggtattttga ttgcaggctg
     1021 ttctttagtc caacgagcga tgcccggctc caccatgtcg aatgctgaaa ttatcggcgt
     1081 actcaatgcc attgatcaca atgaaatcga tgtcggacaa cttgccctgc agaaggcacc
     1141 caccccggaa gtacgctcat ttgcctctcg catggtacgc gaacatgaaa tgatgatgca
     1201 gaacaccgac cggttggccc atcgtttgag cgtgcagccc caaaatccgg ctctcgtctc
     1261 cacgatggcg gatactcacc gggagatgat gaaaaaactc ggtgccaaat atggctctga
     1321 ctttgataag gcgtacatcg gatatcagat caaaatgcat gaacaggcgc tcgaccttgt
     1381 gaaggatgtg gctgaatctg ttgaaaaccg gagccttaag ctgcatctgc gcgagatgag
     1441 gccggacctg cagaaccact tgacttccgc acaatccatc gagcagcagg tcgtcgcgca
     1501 acagtaagaa tccgtgaaga ggagacggac ggaagcagtt gtctcctcac cacggtgcgg
     1561 cgatcggcta gcgaagggac cgtgacaaat tcaccgccga gcatcttgtc cgacacaaaa
     1621 aggcaaacga tatgcccttg ccctatctcc ttcgccttgt tgcagaggga atgtcgtctc
     1681 atctcgaggg atgagacgga caagggtttc ttctagccag ggggcgctat agggtttgct
     1741 atggtttgct attgttgttc cgagaatgcg aatagccgca tgacggactg cgtccgattt
     1801 gaaccgttcg acctgcaatc ctcttccgcc acgcagaggg gatgaagact ggctttggtg
     1861 ggtcccaagc gaatcggctg ccatgaagat taatcggcgc tcgctgcttc gtttctctct
     1921 cgtgtggccg gcgactgcgc cgatgtgggc cggtacgttg ttgcaggcgg ccgaatcgaa
     1981 ggatgatcgg cgaaaggaga cgctcacgaa ggaacgtccg gccgcgacgg acagcctcgc
     2041 cgatgcgatc gtcatcaaga acgagaatgt ctttttcgtg gcacagccgg acggaaacgt
     2101 acccatgaga gactctcatg gcatgggcct ctactatcgc gattgtcgct acgtgaacgg
     2161 ctatgagttg gccctggccg gcaagcagcc ggctcatctc tcggcctcgt cggcccaagg
     2221 ttcgatcggc gtctttacat tgacgaaccc cagaatcgag ctggtgaacg gcgatatcct
     2281 ccatgaagag aaactcggaa ttacatggcg gcggctcatg gaccacgaaa ctccggcgct
     2341 gcgcgattgt ctgctcatcc agaactttac gctccaggcg ctcgagtttc ccgtcagcct
     2401 gacgatccaa tccgccttcg aggatatctt tgccgtccga gggctgctgc cgaagcagtt
     2461 cggcaccctt catcggccgt cctgggaagc cggcacgttg ctgtgggtct acgatggtaa
     2521 agacggcgtc gtccgcactc tcacggtcca cttcgatccg ctccccgaca agcatcaggg
     2581 cacgacggcg gagttcgccg tgcgcttgag cccgcgcggt caacagcgga tcgatgtctc
     2641 gctcgtgatc ggcgaatcca agaaccggac gacggcccgc cgtatgaggc ccgatccggc
     2701 ggacttcagt cgatcggaga gcgagctcga acgagaaacg caggtttgga tggcgcatgt
     2761 caccagggtg gcgaccaacg gctcggccct cgaccaactc atggagcgct ccttccgtgg
     2821 gctccgggtt ttgcgcacac atctgggtga cgagaccttt tttgctgcgg gagtgccgtg
     2881 gttcgttacc ctattcgggc gcgacagcct gatcaccgcc tggcaaatgt tggcct
//
LOCUS       DATNDD010000399        12796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2458473, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000399 DATNDD010000000
VERSION     DATNDD010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2458473"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="lpxD"
                     /locus_tag="VN647_09680"
     CDS             <1..648
                     /gene="lpxD"
                     /locus_tag="VN647_09680"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWG97341.1"
                     /translation="TIGDDCVLYPNVVVREGCSLGARVIVHSGTVIGADGFGYVQHHG
                     RHHKIPQLGGVVIEDDVELGANVTVDRATFGRTLVKQGTKVDNLVQIAHNVTVGEHCI
                     LVAQVGIAGSTTIGRHVMIGGQAGLADHLTIGDQVMIAAKSGVNRSIESNQMVGGIPA
                     MPQEKALRIQGGIFQLPAMKELVRNLERRVATLETQLKKSSGRAPRSNAARSKKR"
     gene            complement(645..1193)
                     /locus_tag="VN647_09685"
     CDS             complement(645..1193)
                     /locus_tag="VN647_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97342.1"
                     /translation="MPKLINVHHPEHTMQQAQGYIKTHILLPSGLLGLICMIGAIGGL
                     GYQWLAIDGYSLSTFYHSSALFMSGVGLGVLQTLYQRYLLREFPEVLAARMKEGLNRQ
                     RGTLKKRSDATTIEHPGRQFIPLAYVLGAMILIGGTIAASAYGQVSIVPALLMPWAGF
                     YWIRLFLWRRVIKVEKAGAVKS"
     gene            complement(1305..2357)
                     /locus_tag="VN647_09690"
     CDS             complement(1305..2357)
                     /locus_tag="VN647_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWG97343.1"
                     /translation="MGDVLTYHEAEYALGEIATAIQEDRSISLEPLETLARDVVSSLQ
                     HNDALVVRALAGPAGSPLITNLINVAILGTKVGIGLGYYGEELHRLALAGFVHDIGLF
                     AVPKSLITKAGRLTQEERALIERHPELGYQVVAKCGPAYHWLAQLTRQAHERFNGQGY
                     PNRLAGREIGEIAQILGVVDVFDALVSDRPYRRRLLPHEAVKELLVAERRTFPREILK
                     ALVEQFSMYPLGTTVRLTNGGVGTVTKVNSCYPLRPVVRMNDQQECEGSSSCEIDLSR
                     MPLVSIAEVLHPPIVGRVTFQEAPSKSAIPVVSTAVSDSFTSLLESLDSVASTLQRVV
                     DTKRNPLNPPDLGTDH"
     gene            complement(2676..3614)
                     /locus_tag="VN647_09695"
     CDS             complement(2676..3614)
                     /locus_tag="VN647_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97344.1"
                     /translation="MSVETEDAFQKELVELFVQEAQEWLQQIHVALDELQQAPPFERH
                     RVLAQTLKVGITNLGGSAATLNLSDVERASFAALPFVEAVQDPSAKISADDFIALCKQ
                     LGYVHRTLTRATGVAFDEDHAPAPDEDPPLMIATKALLALLRGLQDQRTASNPFHRNV
                     VQTMLAQAEGLMERGVEQCNVASIQEFLDRSAEGDQGFLQVVQQELPRLTAVLQTLKN
                     DGVVPGPPSSDLHNAAERVAQLWSAAQQVNASHATVFFMGLHSFLTVVMQHRMVVSAK
                     NYDAIQSRLAQSAKGIEEWAESGRMERMAIQSVLPH"
     gene            complement(3669..5015)
                     /locus_tag="VN647_09700"
     CDS             complement(3669..5015)
                     /locus_tag="VN647_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97345.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGLVVTLAANGEEALAYLAKE
                     RPDVVVSDVIMPDKSGYEVCAFIRGNASLAATPVLLISGIVNDEVTRQAESCRADGVL
                     KKPFQGTSLKDRVLELIAKQQEPESAAVDGPASVPAATPADKMMRVSDQPSLGLSQEA
                     GKLKEIEEQLREERGRCKELSTRLSESTEQLARAKESEAQLATERQRADDLQATLVKV
                     EDQLREARGRSEELTTRLSESTEHLARAKENEAQLATERQRADDLQETLAKVEHQASR
                     IPELESALKTEQEAVDVFKQEAANWQKASGRIAELESALHAERAAAEQLVQQLAELEQ
                     VAIRSRDMEARLASAEQQGGELHRKLQDLEAELLAERRKGEKSTARLHELEQAATRVQ
                     EMERLLTAEQDRNGMLARRVMEAEQGAENATKRLEEMARKLSEIAGLASQLGHGKG"
     gene            complement(5054..5500)
                     /locus_tag="VN647_09705"
     CDS             complement(5054..5500)
                     /locus_tag="VN647_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWG97346.1"
                     /translation="MLRIGHKHHRDAVRLPWQLLVFSVGRKRLAVQTSEVSGISQWNE
                     SIPIASRTPFVTALIRQGQTVLPVFDLAASLHLTVQGNSPLCLKVKHPLGEMAICVDE
                     EIPVLHTFDPAAIQTYHGKDLPAEGSYTNGLDEIPILSVPRLGSTV"
     gene            complement(5494..6060)
                     /locus_tag="VN647_09710"
     CDS             complement(5494..6060)
                     /locus_tag="VN647_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWG97347.1"
                     /translation="MRLVTENEQEGLGMDVRESMTGTPSPVSLLRFLIVVVGGRYLAL
                     NAESICGLLTLEETGNVENPTVHGMVYGTLNLADRLSLPHDAGGANTHVVLLSEGETR
                     GSIRVPMVQGLLELRPSQVLPLPLHFCGEERQWYRGIILFAKSIAVVLNTTWVLDAQV
                     SDLESGGAKEALLCLRRPQISVNDSRIC"
     gene            complement(6092..9448)
                     /locus_tag="VN647_09715"
     CDS             complement(6092..9448)
                     /locus_tag="VN647_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWG97348.1"
                     /translation="MSSEFDRQNLISIFVLEASDGLDALTKALHPPDGAVPTPQELVD
                     QYIVAHRICGAAALYGFSGVAQLAEYLETLLEQATPIPTAEWNRAVDSMRNITQSLRV
                     IVRTIGQGGTEDAEAVERCWASIKQREGETSDATASASSSSTIDEDYLFPALDAEILS
                     YFIPEAEEYLATIDELIRVLREKRDDADSIYRLFRAAHTLKGSAYTVGFHVIGDVARP
                     MEDCMVSVHEHRLPLGNDLLDMLAKAAELIRLILRHESANTQRLRHDIPLLLNCLIQV
                     AEGTAASPAATDQSHRTDIGGSNSVQHTPVVGLEVPAADLSDRYLVPDLDPEVLSYFI
                     PEAQEYLELLEANLLRLDKDPQNKELINPLFRSAHTLKGSAYTVGFQSIGDLVHHVED
                     FMGSVRDGSLSVLPGHTDLMLRAIDVVRALMRRDLDKVDDTRQRFESARTDLRRLDQG
                     VISETTPAHQQDDTSGTTAAGPSGGEESHAQDDGAPNEKSREEREVIRVSYARLERLM
                     NLVGELVIGRGRLEQRLRVLEQLSQQVLVFRSRLVDSVQSFADKHTFSYQDTSSSSTA
                     PASQGLPVFGDFGSLELDKYDDFNILARRIGEVTADISESMSQLDGSIQRTHDEMSQL
                     QHLTLLMRDEIAHARMVPIGTPFTRFRRAVREIARASNKEASLITSGEQTEVDTGVVE
                     RLVDPLVHLVRNAVYHGIEPAADRVAKGKPAIGTVYLHAVHRGNSVIIEVEDDGAGLD
                     MDKIRAKAVKIGLVQSHRIHTMSDADLLQLIFMPGFSTADRIGDQAGRGVGLDVVKRV
                     IEGMNGHIDVESEPGVGTKFTLNLPLTLLIATALFVRVGTAKYAIPLLSIQEVTMPTL
                     SAVREEDDRKLLQIDEQVIELQSLYYILRREPGNVDWTMPVVIVRTAGGPIGLSVDEL
                     LGRQEIVIKPLGPLKPLEHSFFGGATIDQEGRVVLVIDPSRLPSSGTKESAARSPFPE
                     TRPLPEPSLRQEEMLSDKPQARLLLIDDSLSIRKFVGRMLESAGYPFDTAVDGEDGLR
                     KASAISYRMILTDLEMPKLNGFEVIQALRSRPETRQTPVVVMTTRARDKHRQMAMSLG
                     ANSYIPKPVEEQMLLHEVERWIGKAPALRK"
     gene            complement(9503..11392)
                     /locus_tag="VN647_09720"
     CDS             complement(9503..11392)
                     /locus_tag="VN647_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWG97349.1"
                     /translation="MAKVRKTWKWSPRAWFKNLKALHKILIGFSVIMIGMMAIGLIGL
                     MGLSQLKGQLSSIYDESTVGVSHIAVSSTNLSLYHNALLSVAEQRQKSDFDEAIIPLA
                     ELKRRTLTPIEAYRPSATHETMDGRRDGQELDVLLFTLREYFASAESAVGALNDSFDQ
                     SLTDEQRQSMRELATTVLSTEVALWHGRSIWRFQWVAQLIRDLASELNDNGQRMAIYL
                     TKVMLGGAVGALLLAIAIGYFLARSIVRNIIYVANVATQAAAGNLQARARLDSDDEVG
                     HMAKAFNIMLDRITALVSTEEERDRMQKQLVQFLVLVSDVGKGDLTKRGEVTADMFGN
                     LADGFNLMIQRFSQLMKQVRQAAERVNTSASKLKDSAGQMAGTARHQADESIRALGAV
                     EQLASSMRQVTDTAGASSEAARQVLNATEAGRLAVQETVHDMQRIQLAVQRMSKQIKA
                     LGDRSLEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESST
                     QATREIADLVKVIQSETQHAVVAMEHETQAVEAGSASALRTGDVFKEISTIAQRSAEL
                     AQSIAAAAADQTVSTDQVGRSIKDFTGGAVATQKATDSARATVEDMVTLAEGLTTSVS
                     QFKLA"
     gene            complement(11462..11974)
                     /locus_tag="VN647_09725"
     CDS             complement(11462..11974)
                     /locus_tag="VN647_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWG97350.1"
                     /translation="MISIGELPQLHSDSVLEQTKSTESLRVCLIALGKETFAIDLRQV
                     REVFEPESITPVPGMPAALVGVTNLRGTIIPLADVRAALGVVSVSVLPKYAVVVRHGT
                     QQVGILVEDVPEIRTIQSDDLVACPVRTAEERLPLFSKAFTTEKKLSSIVEVSRLLAS
                     LDGTVDGQSP"
     gene            complement(12006..12368)
                     /locus_tag="VN647_09730"
     CDS             complement(12006..12368)
                     /locus_tag="VN647_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97351.1"
                     /translation="MSKIMVVDDSYAELQLIESYLKAAQHTVVSFSTADGLEDKIASE
                     KPDLIVLDVVMPGRNGFQACRDLKTDARFKNIPIVLCTSKGQESDKFWGQQQGANGHV
                     VKPFKSEDLLQAVKLALR"
BASE COUNT         2747 a         3647 c         3323 g         3079 t
ORIGIN      
        1 acgatcggcg acgattgcgt tctgtacccc aatgtcgtcg ttcgtgaggg ttgttccctt
       61 ggtgcgcgag tgatcgtgca cagtggaacc gtgatcggag ccgacgggtt cgggtacgtg
      121 cagcatcacg gccgccatca taagattccg caactcgggg gggttgtcat cgaagacgac
      181 gttgaactgg gagccaatgt gacagtcgac cgcgcgacgt tcggccggac cctggtcaaa
      241 cagggcacca aagtcgacaa tctcgtccag attgcccaca atgtcacagt gggagagcac
      301 tgtattctcg tggctcaggt gggcatcgcc ggcagcacca ccatcggccg tcacgtgatg
      361 atcggcggcc aagccgggct tgccgaccat ctcaccatcg gagatcaggt catgatcgcg
      421 gccaaatctg gagtcaatcg gagtattgaa tccaaccaga tggtaggcgg catcccggcc
      481 atgccacagg aaaaagcact aagaattcag ggaggtattt tccagcttcc tgcgatgaaa
      541 gaactggtcc gtaatttaga acggcgcgtg gcaacccttg aaacacagtt gaaaaagtcg
      601 tctggccggg ctccacgatc aaatgcggct cgttctaaaa agcgctagga cttcaccgct
      661 cccgcttttt ctaccttgat cactctccgc cacaagaaca gccttatcca atagaaccca
      721 gcccagggca tcaagagcgc cggaactatg ctcacttgcc cataggctga cgccgcaatg
      781 gtccctccga tcaagatcat cgcgcccagc acgtacgcaa gtggaatgaa ctgacgtccc
      841 ggatgctcga tggtcgtcgc atccgaccgc ttcttgagcg ttcctctctg ccgattcagc
      901 ccttctttca tgcgggcagc cagaacttcg gggaactccc gcaggagata ccgttgatac
      961 agcgtctgca atactcccaa gcctacgccg gacatgaaca acgctgaact gtgatagaag
     1021 gtactcaagg aatagccgtc aatggcgagc cattggtatc ccagacctcc gatcgcgccg
     1081 atcatacaga tgagcccgag aaggccggac ggcaggagga tgtgcgtttt tatgtacccc
     1141 tgggcttgct gcatcgtgtg ttctggatga tgaacgttga tgagcttcgg cacgtggttc
     1201 ctttcgcgat cgggtcaaga cgctcggaaa gcatgccatt ataaccatcc agatctctac
     1261 agcaaagagc agaacagggt cctgggctca ttgattccga tcgactagtg atcggtacca
     1321 agatccgggg gatttaacgg gttcctcttg gtgtccacca cgcgctgcag tgtcgaggca
     1381 accgagtcaa gactttcaag caatgaggtg aaggaatcag aaacggcggt ggacacaaca
     1441 ggaattgccg attttgaggg ggcttcttga aatgtgacac gcccgactat gggaggatgt
     1501 agaacctctg cgatcgatac taagggcatg cgactcaaat caatttcaca agaactactt
     1561 ccttcacatt cctgctgatc gttcatccgc acgaccgggc gaaggggata gcagctattc
     1621 actttggtca ctgttcccac tcctccgttc gtcaatcgca ctgtcgtccc aagcgggtac
     1681 atcgaaaatt gctcgacgag cgccttcagg atttctcgag gaaaggtcct tcgttcagcg
     1741 accagaagtt ccttgaccgc ttcgtgcggg agcaggcggc ggcgataggg tcgatcgctc
     1801 accaatgcat cgaagacgtc caccactcct aaaatctggg cgatttcacc gatctctctg
     1861 cctgcgagtc gattggggta tccctgtcca ttgaagcgtt catgcgcctg gcgcgtcaat
     1921 tgcgccaacc agtgatacgc cggtccgcac ttcgcgacaa cctgatatcc cagctcaggg
     1981 tgccgttcga tgagtgctcg ctcttcctgg gtcaaacggc cggccttggt gatcaaggat
     2041 ttcggcacgg caaataaccc aatatcatgg acaaatccgg ctagtgccaa ccggtgtagt
     2101 tcttccccat agtacccgag gccgatcccg accttcgttc cgagtatggc cacattgatc
     2161 agattggtga tgagcggtga cccggccggt ccggctagtg cccgtaccac aagcgcatcg
     2221 ttgtgctgca gggatgaaac gacgtctcgt gcaagagtct caagaggttc tagtgatatg
     2281 gaacgatctt cctgaatggc ggtcgctatc tcgcccaacg cgtattctgc ctcgtgatat
     2341 gtcaagacat cacccatgcg ccggtattgt ctcgccgatg cggcacttca ttcgcggctt
     2401 tttccaccaa tgagcgtctg atatgacgcg cccccgaaac atgtccgaaa tagagacata
     2461 agtcgcaaag cgagttgatc aatcggcata caccaccggt gtgatgataa atggaggaaa
     2521 tcgcactcgg tgagaatatc ggcagctcgc cccccgcagc tgcgagacga gcaaagatag
     2581 cagacgatga ttttgtaccg catcggcagg cggcattagg tggccttttt cgtttcgaca
     2641 tcccggtcag atcgagcttc tggtacggga cttattcaat gaggcaatac gctttgtata
     2701 gccatgcgct ccatccgtcc gctttccgcc cactcctcaa ttcccttcgc gctctgggcc
     2761 agacgtgatt ggatcgcgtc atagtttttg gcactcacga ccatacggtg ctgcatcaca
     2821 accgtaagaa aactatgcaa tcccataaaa aacaccgtcg catgggaggc attgacctgc
     2881 tgagcggcag accacagctg cgcaacacgt tcagcagcat tatgcaggtc ggaggatggt
     2941 gggccaggga cgacgccatc gttcttaaga gtttgaagaa cagccgtcag acgaggaagc
     3001 tcttgttgaa cgacttggag aaaaccttga tctccttcgg ctgaccggtc gagaaactcc
     3061 tggatggaag cgacattgca ttgttcgacc ccgcgctcca tcaacccttc cgcctgggcc
     3121 agcatcgtct gaacgacatt gcgatggaat gggttggatg ccgtacgttg atcttgaagt
     3181 ccacggagta acgctaaaag cgctttcgtc gcgatcatca gtggtgggtc ttcgtcggga
     3241 gcaggtgcat ggtcctcatc aaatgccaca cctgtcgctc tagtcagagt tctatgaacg
     3301 taccccagct gcttgcagag tgcgatgaag tcatcggcgg aaattttcgc agaagggtct
     3361 tgaacggctt caacaaatgg gagagccgca aaactagccc gttcgacatc gctcaggttg
     3421 agcgtggctg cggatccgcc gaggttcgta atgccgacct tgagcgtttg ggccaagaca
     3481 cgatggcgtt caaaaggggg agcttgctga agttcatcga gagcgacgtg aatttgctgg
     3541 agccattcct gtgcttcttg aacgaacagt tcaaccagct ctttctggaa cgcatcttcg
     3601 gtttcaacgg acatcggctt ctgcccttca actagaccat cgagatgctc agattgaccg
     3661 cgtcaggatt atccctttcc atgtccaagt tgggaggcca atcctgcaat ttcacttaat
     3721 ttgcgtgcca tttcctcaag ccgtttggtg gcgttctcgg cgccttgttc ggcttccatt
     3781 actcgtcgag cgagcatgcc gttgcgatcc tgttccgcag tcaggagccg ttccatttct
     3841 tgaactctcg tcgccgcttg ctccaattcg tgaagtcgcg ctgtgctctt ctcacccttt
     3901 cgccgttccg ccaaaagttc ggcttcgagg tcctgaagtt ttcggtgaag ttcgcctccc
     3961 tgctgttccg cactcgcgag ccttgcttcc atgtctcttg atctgattgc cacctgctcc
     4021 aactccgcca gttgttgcac gagttgttct gccgccgccc gttcagcgtg cagagcggac
     4081 tccagttctg caattcgacc ggaagccttt tgccagtttg cggcttcctg tttgaataca
     4141 tcgactgctt cctgttccgt cttcagtgcc gattccaact cagggattct tgacgcttgg
     4201 tgctctacct tggcgagtgt ctcctgaaga tcgtcggcac gttggcgctc ggttgccaac
     4261 tgtgcttcgt tttccttggc tctggcgaga tgctccgtag actcagatag tcgcgtggtc
     4321 agctcttcgg agcgacctcg tgcttctcgt aactggtctt caaccttaac gagtgtcgcc
     4381 tgaagatcgt cggcacgttg gcgctcggtt gccaactgtg cttcgctttc cttggctctg
     4441 gcgagctgct ccgtagactc agacagtcgc gtgctcagtt ctttacagcg gcctcgttct
     4501 tcccgtaact gttcttcaat ctctttcagt tttccggcct cctggctcag acccagtgac
     4561 ggttggtccg ataccctcat cattttgtcg gcgggagtcg ccgcaggaac ggaagcaggt
     4621 ccgtcgaccg cggccgattc cggttcctgc tgttttgcta tcagctcgag gactcgatcc
     4681 tttagggacg taccttgaaa cggcttcttc aataccccat ccgcgcggca ggactcggcc
     4741 tgtctggtca cttcatcgtt cacgattccg gaaatgagca gcacgggcgt cgctgcaagg
     4801 gatgcatttc cacggataaa tgcacagact tcatatccac tcttgtccgg catgatgacg
     4861 tcggacacaa caacatcggg ccgttctttg gccagatagg ccaatgcttc ctctccgttt
     4921 gccgcgagag tgacgacgag gccggcctcg gtcaatagtc gctcggcaac cttgcgaacc
     4981 gcgatgctgt catcagctat taagatcttt ggcatgcctg cacctttcgt ccatgctgac
     5041 acaaccggaa tcgtcacaca gtcgacccaa gtcgtggtac agagagaatc gggatctcgt
     5101 ccaggccatt ggtataactg ccttcggccg gcaaatcttt tccatggtac gtctgaatcg
     5161 cggcaggatc aaaggtatga agaaccggaa tctcttcatc aacacatatc gccatttctc
     5221 cgagaggatg cttcaccttc agacacaagg gactgtttcc ttgcacagta agatgaaggg
     5281 atgcggccag atcgaataca ggtaacacgg tttgcccttg acgaatgagc gccgtgacaa
     5341 aaggagttcg actcgcgatc ggaatagatt cattccactg cgaaataccc gatacctctg
     5401 atgtctgcac tgccaatcgc ttcctcccca ccgaaaacac caatagctgc cagggtagac
     5461 gtacggcgtc ccgatgatgc ttgtggccga tcctcagcat atccggctat cgttcacaga
     5521 aatttgtggt cgtcgcaagc agaggagtgc ttcctttgcc ccaccgcttt cgaggtccga
     5581 cacctgcgca tcaagaaccc aggttgtatt gagcactacg gcaatgcttt ttgcgaatag
     5641 gattatgccc cggtaccatt gtcgttcctc tccgcagaaa tgcaagggaa gcggtaggac
     5701 ttgagacggt cggagttcaa gaagtccctg caccatgggg actcgaatgc tcccacgcgt
     5761 ttccccttca gagagcagca cgacatgcgt gttcgctcca cccgcgtcat gcggtaagct
     5821 caatcgatcg gccagattga gggtgccata caccatgccg tgaactgtcg ggttttcaac
     5881 atttccagtc tcttcaagag tcaacaaccc acatatagac tcggcattca atgccagata
     5941 ccgtccgccc actactacga tgagaaatcg taatagagaa actggcgacg gagttcccgt
     6001 catcgattcc cgaacgtcca tgccgaggcc ttcttgctca ttctctgtaa caagccgcat
     6061 ttcgtcaata gatacgagta tttccgcaag gttacttgcg cagcgccgga gcctttccaa
     6121 tccatcgttc cacctcatgc agcaacatct gttcttctac cggcttgggg atgtatgaat
     6181 tggcaccgag gctcatcgcc atctgccgat gtttgtctcg agccctcgtc gtcatgacaa
     6241 ccaccggcgt ttgcctggtt tccggacgac tcctgagggc ttgaataact tcgaatccgt
     6301 tgagtttcgg catttcgaga tcggtgagga tcatccgata acttattgcc gatgcttttc
     6361 ggagcccatc ctcgccgtca accgccgtat cgaacggata tccggccgac tccagcatcc
     6421 tgccgacgaa ctttcgaatg cttaatgagt cgtctatcaa gagcaggcgc gcctgcggct
     6481 tgtccgataa catctcctct tgccgcaagg acggttctgg aagtggtctg gtctcgggaa
     6541 agggagaacg agcggccgat tctttcgtcc cactcgacgg caaacggctt ggatcgataa
     6601 caagaactac ccgcccttcc tgatcaatgg ttgctcctcc gaaaaacgaa tgttccaacg
     6661 gtttcagtgg tccaaggggt ttaatgacaa tttcctgacg gcccaacagt tcgtcgaccg
     6721 ataacccgat cggtcctccc gccgtgcgga cgatcaccac cggcatggtc caatcgacgt
     6781 ttcctggttc ccgacgaagg atgtaataga gagattgtaa ttcaataact tgttcgtcga
     6841 tctgtaagag cttacgatca tcttcctctc gtactgcgga tagcgtcggc atcgtcactt
     6901 cctgaatact cagcaatgga attgcatatt ttgcggtacc gacccgcacg aacaacgcgg
     6961 tcgcgatcag gagggtaaga gggagattca aggtaaactt ggtgccgacg ccgggttcag
     7021 attctacatc gatatgcccg ttcatacctt caatgactcg cttcaccacg tctagtccta
     7081 cgcctcgacc tgcttgatcc cctatcctgt cggctgtcga gaaaccgggc atgaaaatta
     7141 actgaaggag gtccgcatcc gacatcgtgt gtattcggtg agattgtacg agcccgatct
     7201 tgacggcttt cgcccgtatt ttatccatat ccagccctgc tccatcatct tccacctcaa
     7261 tgatgaccga gttcccccgg tgaactgcgt ggaggtagac ggttccgatg gctggtttcc
     7321 ctttcgcgac tcgatcggcc gcgggctcga tgccgtgata aacagcgttc ctgaccaaat
     7381 gcaccaatgg atccaccaat cgttccacga ctccggtatc aacttctgtc tgttcgcctg
     7441 atgtgatcaa ggacgcttcc ttgtttgaag ctcgagcgat ttctctgacc gctcgacgaa
     7501 accttgtgaa cggtgtaccg atcggaacca tgcgggcatg ggcgatttca tcccgcatca
     7561 acagcgtcaa gtgctgcagt tggctcatct cgtcgtgggt tctttggatg gacccatcca
     7621 actgcgacat cgactcactg atatcggcgg tgacctcccc gatgcgccga gccaaaatat
     7681 tgaaatcatc gtacttgtcc agctcgagac tgccaaaatc accgaatacg ggaagtcctt
     7741 gactcgcagg cgcggtcgaa ctactggatg tgtcctgata gctgaaggtg tgcttgtcgg
     7801 caaaggactg aacggaatcc accaaccgac tcctgaatac caacacttgc tgtgacagtt
     7861 gctccaggac tcgcaatcgt tgttccaatc ttccgcgtcc aatgacgagt tctcctacga
     7921 gattcatcaa tcgctcgagc cgtgcataac tgacacggat gacctcgcgt tcctctcgcg
     7981 atttctcatt cggagctcca tcatcctgtg catgagactc ctcacctccg gacggtcctg
     8041 cggccgttgt accggaggtg tcatcctgtt gatgtgccgg cgtcgtctcg cttatgacac
     8101 cctgatccaa tcgtcggagg tcggttcgcg ccgattcgaa tcgttgcctt gtgtcgtcca
     8161 ccttgtcgag gtctcttcgc atgagtgctc gtacaacatc gatcgcgcga agcataagat
     8221 ccgtatgccc gggtaatacg ctgagactcc cgtctcgcac cgagcccatg aagtcttcga
     8281 cgtgatggac caggtcgccg atggactgaa acccaacggt ataggcggag cctttcaagg
     8341 tgtgcgcgct tctgaacaac ggattgatca gttccttgtt ttgcgggtcc ttgtccaatc
     8401 gcaggagatt ggcttccaag agttccagat actcctgtgc ctcagggata aagtaggaga
     8461 gcacttccgg atccaaatcc ggaaccaagt acctatcgga taagtccgct gcaggcactt
     8521 ctaggccaac aacaggcgta tgctggacag aattcgagcc acctatgtcg gtgcggtggg
     8581 attgatcagt cgctgcaggt gaggcggctg taccttcagc cacttggatg agacagttga
     8641 gaaggagtgg tatgtcgtgc cggagacgtt gcgtattcgc cgattcatga cgaagaatga
     8701 gccggatcaa ttcggcagcc ttcgcaagca tgtcaagaag gtcgttgccg agaggaaggc
     8761 gatgttcatg aaccgagacc atgcaatctt ccatcggacg agcgacatct ccaatgacgt
     8821 ggaagcccac ggtgtacgct gagcccttca gggtatgcgc cgcgcgaaag agccggtaga
     8881 tcgagtctgc atcatcccgc ttttctctaa ggacacgaat caactcgtca atggtggcga
     8941 ggtattcctc agcttccggg atgaagtagg ataagatttc ggcatcgagc gccgggaaca
     9001 ggtagtcctc atcgatcgtc gacgaggagg acgcggacgc tgttgcgtcg ctcgtttctc
     9061 cttctcgttg ttttatcgac gcccaacagc gttctacggc ttctgcatct tcagtgccgc
     9121 cttgtccgat ggttcgaacg attactcgaa ggctttgcgt aatgttccgc atcgagtcta
     9181 cagctcggtt ccattcagct gtaggaatcg gcgtcgcttg ttcaagcagt gtctctaagt
     9241 attcggcaag ctgagcgact ccgctgaagc catacagggc cgcggctccg caaattctgt
     9301 gagcgacgat gtattgatcg acaagttcct gaggagtggg aacagcaccg tcgggagggt
     9361 gtaatgcctt ggtcagggca tccaatccat ccgatgcttc aaggacaaaa atgctgatga
     9421 gattctgcct gtcgaactcc gagctcatcg atccctcttt acaccggaag gttcctcctc
     9481 actcagggga tgagggagat tctcaggcca gcttaaattg tgacaccgaa gttgtcagac
     9541 cttcggccaa cgtcaccatg tcctctaccg tcgcacgcgc agaatcagtg gccttttgtg
     9601 tcgccacggc acctccggta aaatccttga ttgaacgtcc gacctgatcg gttgaaaccg
     9661 tttggtcagc cgccgccgcc gctatgcttt gagcaagctc tgcggaccgc tgagcaatcg
     9721 tcgaaatttc cttgaacaca tcgcccgtac gcaaggccga agccgacccg gcttcgaccg
     9781 cctgagtttc atgctccatg gcaacgaccg catgttgcgt ttcgctctgg atgactttca
     9841 ccaggtccgc gatctcgcgt gtcgcctgcg tcgagctctc cgcgagcttc cgcacttgat
     9901 cggcgaccac ggcgaagcgt gctccggcct caccggcacc ggcggcctca atcgcggcgt
     9961 tgagagccaa aaggtttgtt tgattggcga tgtctcgaat ggtcgatacg atctgtgaaa
    10021 tttccaatga acggtcgccg agcgccttga tctgtttcga catacgctga accgccaatt
    10081 gaatgcgctg catatcgtga acggtttcct gcaccgcgag ccggccggct tcggtggcgt
    10141 ttaagacttg gcgggctgct tcggacgagg ctcccgctgt gtccgtcact tggcgcatcg
    10201 atgacgccag ctgttcgacg gcgccgagtg ccctgatcga ttcgtcggct tggtgtctgg
    10261 cggttccggc catctgtccg gcactgtcct tcagcttgct cgccgaggta ttgacgcgtt
    10321 cggctgcctg acgcacttgt ttcatcaact gtgaaaagcg ctgaatcatg agattgaatc
    10381 cgtccgccag atttccaaac atatcggccg tcacttcacc gcgcttggtc aaatctcctt
    10441 tcccgacgtc ggagaccaac acaaggaact gcacgagttg tttttgcatt ctgtcacgtt
    10501 cctcttccgt cgaaaccaag gccgtgatcc gatccaacat gatgttgaac gccttggcca
    10561 tatggccgac ctcgtcgtcg ctgtccagcc gagcgcgggc ctgcagattt cccgcggcag
    10621 cctgtgtcgc gacattggcg acgtagatga tgtttctcac aatgctgcgc gcgaggaaat
    10681 atccaatcgc gatcgccaag agaagtgccc caaccgctcc accgagcatg accttggtaa
    10741 ggtatatggc catgcgttgc ccgttatcat tcaattcact cgccaagtct cgaatgagtt
    10801 gagccaccca ttggaagcgc catatcgacc ggccgtgcca caaagccacc tcggtcgaca
    10861 gcaccgtggt agccagttcc ctcatcgact gccgttgctc atccgtgagc gattggtcaa
    10921 aactatcgtt caacgccccc acggcacttt cagccgatgc gaagtattct ctcaacgtaa
    10981 aaagcaggac gtccaactct tgtccatcgc gacggccatc cattgtctcg tgcgtggcgg
    11041 acggtcgata tgcctcaatc ggcgtcaggg tgcgcctctt caattcggcg aggggaatga
    11101 tggcctcgtc gaaatcactc ttttgtctct gttccgcaac gctcaacaac gcattgtgat
    11161 agaggctgag attggtgctg ctcaccgcga tgtgcgaaac tcccactgtt gattcgtcat
    11221 aaatggaact cagctggccc ttcagctgac tgaggcccat caaaccgatc aggccaattg
    11281 ccatcatgcc gatcatgatg accgaaaacc ctataaggat cttgtgcaag gctttcaggt
    11341 ttttaaacca tgcacgaggc gaccatttcc atgtcttcct aaccttcgcc atgattttcc
    11401 tcctgctcgc tgctcgcgat agggcttaca tttgccgaca caatcgttcc tcaatatgac
    11461 gttatggact ctggccatcg accgttccat ctagggacgc caagagccgc gacacttcca
    11521 ctatggagct cagtttcttc tctgttgtga aagccttaga aaacaacggc agtctttcct
    11581 ccgcggtacg aaccgggcag gcgactagat catccgactg gatcgtacga atttccggta
    11641 catcttcgac caggataccg acttgctgag tcccatgacg aacaaccacc gcatatttcg
    11701 gcaggacgga aacggatacg acgcccaaag ccgccctcac atccgcgaga ggaatgatgg
    11761 ttccgcgtag attggtgacg ccgaccagcg cagcaggcat tcctggtaca ggtgtgatcg
    11821 actcaggctc gaagacctcg cggacttggc gtaggtctat ggcgaaggtt tcttttccca
    11881 gtgcgatcag acaaaccctc aatgattcgg tggatttcgt ctgttccagg actgagtctg
    11941 aatgaagttg aggcaactca cctatggaaa tcatcgtttg gcggttccgt gcaatatcgg
    12001 cgctctcagc gcaatgcaag cttcacagct tgtagaaggt cctctgattt gaagggcttc
    12061 accacatgtc cgttggcccc ctgttgctgt ccccagaact tgtcgctttc ctgtcccttc
    12121 gaggtgcaaa gcacgatcgg aatgttcttg aagcgagcat cggtttttaa atccctgcac
    12181 gcctgaaatc cattccggcc gggcatgacc acatccaaga cgatgagatc gggtttttca
    12241 gaggcgatct tgtcctccaa cccatccgcc gttgaaaacg atacaacggt gtgttgggca
    12301 gccttaagat acgactcgat caattgcagt tctgcatacg agtcatcgac aaccatgatt
    12361 ttgctcatac acccttcctc tctcttcagt aaatgtcggc gctgtcccaa tgcgtcggca
    12421 tcgccacact ggttgcgcag gccggttctt tctcttctct tgcgcgcttg tgcatgtcag
    12481 ccaacgcgtg cacgcttctt gggttgctag gcataccgga catgcggcat cagcaacatt
    12541 ctcgtctccg aggcatttct tcctatcctt acaaagtcca ttgccgctgt tctcaagcag
    12601 tgtaacgact tgttgatggt tggcaagaaa acggtgaata tcggcaccgt tttgaagaag
    12661 ttggaggtga tgctatcgtg agaatccgtg aggcccgtat cgggaaggaa ctagcagttt
    12721 gttgacaaac tgcggttttt aaagaattcc tgcatcgaat tcaccagttg attctccgat
    12781 gatggtcttg tgtgtc
//
LOCUS       DATNDD010000400         4548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_811133, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000400 DATNDD010000000
VERSION     DATNDD010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811133"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VN647_09735"
     CDS             complement(<1..349)
                     /locus_tag="VN647_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97352.1"
                     /translation="MKLRLVPQLSQKLIMTPQLQQAIKLLQLSRLELQQSLTQHLLEN
                     PLLDEIQSDVDEGESLGNEEKVEAPPALEGQDRSDAAVETREEQGSPEEFSASGWEEY
                     FGRDRRSGESEYSA"
     gene            complement(369..1151)
                     /gene="lptB"
                     /locus_tag="VN647_09740"
     CDS             complement(369..1151)
                     /gene="lptB"
                     /locus_tag="VN647_09740"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWG97353.1"
                     /translation="MTRSAHAESDELVDSIAVRRGDCLRATGLVKSFRGRKVVKGVAV
                     EVYAGEVVGLLGPNGAGKTTIFDMMVGLCQPDEGEITFIGESVTNLPMYKRARRGIGY
                     LPQESSVFRRLSVEHNVLAILEMLGYARKERSKRVDALLKELDLIHIRKSMAYALSGG
                     ERRRLEITRALAATPSFMLLDEPFAGIDPIAVADIQQIITRLKGKGIGILITDHNVQE
                     TLSIVDRAYIINEGLILEAGPPEAIVQSATARAVYLGEQFKL"
     gene            complement(1148..1663)
                     /locus_tag="VN647_09745"
     CDS             complement(1148..1663)
                     /locus_tag="VN647_09745"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HWG97354.1"
                     /translation="MEAPGVPTTITSKKMTVRNQDSQAVFEETVVLTRGPLIVHSDKM
                     VVLFTPRNPDPLQSARGGTDQHESVRNFSAPKEHNTASTMSNRSVSRVEATGDAHRVK
                     IKYENGNATCQKAVYFVDGEKIVLTGDPVAWEKGTRVSGKQITIFLTEERSVVEGGSH
                     VRIEGEGQSHP"
     gene            complement(1728..2264)
                     /gene="lptC"
                     /locus_tag="VN647_09750"
     CDS             complement(1728..2264)
                     /gene="lptC"
                     /locus_tag="VN647_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWG97355.1"
                     /translation="MSVLLATFLGYLLFRNADSVPSSLALAPGSIEQADAKIEEFIFT
                     QSKGDVVQWQVQAKQARLFEQDKRAVLRDVAFTFYGTGGDEVTVHGEEGTLDTATKNF
                     RLVNRQTPIVVETRSGYTIYTNHLVWMDQAREIRTEDPVRMVGHGLEVTGQGLLGRME
                     SEEFEVLQDVHVDLVPSS"
     gene            complement(2463..2894)
                     /gene="rplQ"
                     /locus_tag="VN647_09755"
     CDS             complement(2463..2894)
                     /gene="rplQ"
                     /locus_tag="VN647_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWG97356.1"
                     /translation="MRHRKKGRQLGRQTKHRKALFRNLVTSLLDQERIETTGAKSKEI
                     RGFTDRMITLGKEGTLPARRRALGFLRSKTVVSKLFSDVATRFKDRSGGYTRIVKTRR
                     RIGDAAEMVAIELVSRQELTTKKKSDARTSQSAPTGAGTSA"
     gene            complement(2919..3908)
                     /locus_tag="VN647_09760"
     CDS             complement(2919..3908)
                     /locus_tag="VN647_09760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWG97357.1"
                     /translation="MKDFQIPMRVEVDKDAHSPTFGRFTTEAFERGFGTTVGNALRRI
                     LLSSLTGAAVTTVKIEGVVHEFSTISGVTEDVTAIILNVKSLRLALHTDKPKTIRLRK
                     KGPGEAKGADILHDGDVTILTPDLHIATLDKDATLDIEMTVKHGRGYVPAERNKEEGL
                     PIGVIAIDSIFSPIRRVNFHVENARVGRMTDYDKLTMEIWTDGTISPRDALSTGAGIL
                     REHLDIFINPEERSEGKSEAGYEESHREVNKNLSRSVNELELSVRAANCLKNANIKTI
                     ADLVQKSEGEMLRTKNFGKKSLNEIKEILSEMGLSLGTKIEAASPHNGSPKPE"
     gene            complement(4002..>4548)
                     /gene="rpsD"
                     /locus_tag="VN647_09765"
     CDS             complement(4002..>4548)
                     /gene="rpsD"
                     /locus_tag="VN647_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWG97358.1"
                     /translation="TEKCAVERRSYPPGQHGQGRQRTSDYSLQLREKQKLRRIYGLQE
                     CQFRGVFERAERQTGVTGDALLRLLECRLDNVAYRLGFGASRKQARQMVSHGHFTVNG
                     KKITVAGALVKPGDVIEIRERSRDLAAIQAALESVDSRGIPDWLELDKGAFKGTVRAL
                     PAKEQIALPVNEQMVVELYSR"
BASE COUNT         1011 a         1353 c         1060 g         1124 t
ORIGIN      
        1 ccgctgagta ttcagactcg ccgctccgtc gatccctgcc gaagtattct tcccacccgg
       61 aagcggaaaa ctcctctggc gacccttgct cttcacgtgt ctccactgcc gcgtcagacc
      121 gatcctgccc ttccaatgcg ggaggggctt caaccttttc ctcgttgccc aaggactcgc
      181 cttcgtcgac atcggattga atttcatcca gcaaagggtt ttcgaggaga tgctgagtga
      241 ggctctgctg cagctcaagt cgagacaact gcaacagctt aatcgcttgc tgcagttgag
      301 gcgtcatgat gagcttttgc gagagttgtg gaaccagacg gagtttcatc gatgccttta
      361 ttgcacttct acaacttgaa ctgctcgcca agataaacgg ctcgggccgt ggcactctgc
      421 acaatagctt ccggaggccc cgcctccaag atcaatcctt cattgatgat gtaggcacgg
      481 tcaacgattg aaagcgtttc ctggacattg tgatcggtaa tcaatatgcc gatgcctttt
      541 cccttcaacc gggtgatgat ctgctgaata tccgcgacgg ctatcgggtc gatccctgca
      601 aacggttcat ccaacagcat gaacgacggt gtggccgcca acgcacgcgt gatttccaaa
      661 cgccgacgct ctccccccga gagggcatag gccatactct ttcgtatatg gatgagatct
      721 aattccttga gcaaggcgtc cacccgcttg cttcgctctt tacgagcata ccccagcatt
      781 tcaagaatcg ccaagacatt atgttctacc gagagccgcc gaaaaaccga cgattcctga
      841 ggcaggtaac cgattcccct acgcgctcgt ttgtacatcg gcagattggt cacggattcc
      901 ccgatgaagg tgatctcccc ttcatccggc tgacacaagc caaccatcat gtcgaagatt
      961 gtggtcttgc ccgctccatt cggaccgagc aaaccaacca cctcgccggc atacacctcg
     1021 acagcgacac ccttgacgac tttgcggcca cggaaacttt ttaccaatcc agtcgcacgc
     1081 agacaatccc cccgccgtac cgcaatcgaa tcgacgagct cgtcagactc tgcatgagca
     1141 ctccgggtca tggatggctc tgcccctcac cttcgatgcg aacgtgtgag cctccttcta
     1201 ccacgctccg ctcttctgtc aaaaaaattg taatctgttt accgctgaca cgcgtgccct
     1261 tttcccaagc tacaggatcg cccgtcaata caatcttctc gccgtctacg aaatatacgg
     1321 ctttttggca agtggcattg ccgttctcat acttgatctt gacacgatgg gcgtcaccgg
     1381 tcgcttcgac acggctcacg gatcgattcg acatcgttga cgccgtgttg tgctctttgg
     1441 gagccgagaa attccgaacg gattcatgtt gatccgttcc tcctcgggct gactgcaggg
     1501 ggtcagggtt tcggggagtg aacaacacga ccatcttatc agagtggacg atgagaggcc
     1561 ccctggtcaa tacaactgtt tcctcaaaaa ccgcctggct gtcttggttt cgcactgtca
     1621 ttttcttgga tgtgatggtg gtcgggacac cgggcgcttc catgctcctc ttcaggttca
     1681 cagattccgt tgagggagcc gccgaggtgg atccgacaaa aaagacacta agaagaagga
     1741 accagatcca catgcacatc ctgtagaacc tcaaattctt cggattccat tcgcccaagc
     1801 aatccttgcc ccgtcacctc aagcccgtgc cccaccatcc gtacgggatc ttccgtccga
     1861 atttctcgtg cttggtccat ccataccaag tggttggtat aaatcgtata gccgcttcta
     1921 gtctccacaa caatgggcgt ttggcgattt accaacctga aattcttggt tgcagtatcc
     1981 agcgttccct cttccccatg cacggtcact tcatctcctc ctgtgccgta gaaggtgaag
     2041 gcaacgtcac gcagcacggc acgcttatct tgctcaaata gtcgagcctg cttagcctgt
     2101 acctgccact ggacgacatc acctttcgat tgggtaaaga tgaactcttc gatcttggca
     2161 tccgcttgtt caatcgaccc tggagcgagg gccagactac tcggcactga atctgcattt
     2221 ctgaagagaa gatagccaag aaatgtggct aataacacac tcacggcgag aagagtccgc
     2281 ctcgctacaa gctcccacat gctctagaaa tttcccccgg gtagatgcat agcgatacct
     2341 ggcacgatcg tagcatgcac ggcctgccaa gtaaacgtcc tccgggaact ggaaataaaa
     2401 tgactcccat gtgttggcac atgggagttt cagatgagca gctaaatttt cgtgactaaa
     2461 aactatgccg atgttccggc gccggtgggt gcagactgag aagtacgagc atcggacttt
     2521 ttctttgtgg ttagttcctg acgagaaact aactcgatag caaccatttc agccgcatcg
     2581 ccgatacggc gacgagtttt gacgatcctg gtgtatcccc cggatcgatc tttgaatctt
     2641 gtcgccacat cgctaaacag tttagataca acggtcttgc tgcgaagaaa tcctaaagcc
     2701 cgtcggcggg caggcagcgt accttctttg ccaagggtga tcatgcgatc ggtaaaccct
     2761 cgtatctctt tagatttggc cccggtggtc tcgatacgct cttgatccaa caacgaggtc
     2821 actaagttcc gaaacaacgc cttccgatgc ttcgtctgcc gccctagttg tcgacccttt
     2881 tttctatgtc gcacggtagt cctcttgatt ggaaagcatc attcaggctt tgggcttcca
     2941 ttgtgtggag acgcagcctc tatctttgtt cctaacgaaa gccccatctc agaaagaatt
     3001 tccttgatct cattgaggga ttttttcccg aagttctttg tcctgagcat ctccccttcc
     3061 gacttttgca caaggtcggc gattgttttg atattcgcat tcttcaggca attagccgct
     3121 cgaacggaca actccaactc attcacgcta cgagacagat tcttgttcac ttcccggtgc
     3181 gattcttcat aacccgcttc actcttccct tcgctgcgct cctccggatt gataaaaata
     3241 tccagatgtt cacgcaaaat cccggccccc gtagagaggg cgtcgcgagg gctgatggtg
     3301 ccgtcagtcc agatctccat cgtcaattta tcatagtcgg tcatacgacc gacccgcgca
     3361 ttctcgacat ggaaattgac tctcctgatc ggagaaaaaa tggaatcgat ggcaatcacc
     3421 ccgatcggca atccctcctc tttgttccgt tcggccggca catagccacg gccgtgtttc
     3481 accgtcatct cgatgtccag agtcgcatcc ttgtctagcg tggcgatatg caaatcaggt
     3541 gtcagaatcg tcacatcgcc atcgtggaga atgtctgccc ctttcgcttc tccaggcccc
     3601 ttctttctca accgtatcgt cttcggttta tccgtgtgga gcgcaagccg caaactcttg
     3661 acatttagaa tgattgccgt gacatcttct gtcacaccag agatcgtcga aaactcgtgc
     3721 acgacccctt caatcttgac ggtggtcacc gcagcccccg tgagcgacga cagcaaaatg
     3781 cgacgaaggg cattgccgac cgtggtgcca aatccccgtt caaacgcttc cgtagtgaat
     3841 cgcccaaatg tgggagaatg tgcatcctta tcgacttcca cccgcattgg gatctgaaag
     3901 tctttcatcg ctttgatcat gacccaccct ccataccatc gagtgactaa gacatcgaca
     3961 gataacgccg atgtcgaaag aacgcacctc agatcggcgg actatcgcga gtataattcc
     4021 actaccatct gttcgttgac cggcaacgcg atctgctcct tggccggcaa tgcgcgaacg
     4081 gtgcccttga acgctccttt gtcgagctca agccaatcag gaatgcctcg actatcgacc
     4141 gattccaatg ccgcctggat tgcggccaag tcccgactcc gttcgcgaat ttcgatcaca
     4201 tctcccggct tcaccagtgc cccggccacc gtgatcttct tgccgtttac agtgaagtga
     4261 ccatgactca ccatttgacg agcttgcttg cgcgacgcac cgaaccctaa gcgataggcg
     4321 acattgtcca agcgacactc gagcaaacgc aacaaggcat cgcctgtgac gccggtctgc
     4381 cgttctgcac gttcgaaaac gccgcggaat tggcattcct gaagtccata gattcgacgc
     4441 agcttctgtt tttcgcgcaa ctgcagacta tagtcagaag ttcgctgtcg tccctgccca
     4501 tgttggccag gcggataact cctgcgttcc acagcgcatt tttccgtc
//
LOCUS       DATNDD010000401         6475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1627681, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000401 DATNDD010000000
VERSION     DATNDD010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627681"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VN647_09770"
     CDS             <1..219
                     /locus_tag="VN647_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWG97359.1"
                     /translation="SVVLDRVGILLQMMQIAAARVVIVSNELGFGLVPTEPSTRAFRD
                     LAGRVNQQIAAGADEVYLVVSGLPLRLK"
     gene            234..1286
                     /gene="cobT"
                     /locus_tag="VN647_09775"
     CDS             234..1286
                     /gene="cobT"
                     /locus_tag="VN647_09775"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWG97360.1"
                     /translation="MPIQDLCRLIQPLNASLHGKAQARLDRLTKPLGSLGRLEELAAS
                     YVAITGELKPNVPRAVVFTFAADHGVALEGVSAYPREVTPQMVLNFLRGGAGVNVLAR
                     HAGVDVRVVDIGVDYEFGAVPGLLDRKVMKGTRNLAVESAMTRSQAEQAVMVGVELAT
                     EAVREGIGLIGTGEMGIGNTTPSAAITAVMTGRPAEEVTGRGTGVDESGRMHKAAVIQ
                     RALELHRLNPTDPLDVLAKVGGLEIGGLAGLMLGAAAVRVPVVLDGFIAGAAALIAVG
                     LQPLCREYLIASHRSVEQGHRAVLDHLRLKPLLDLDLRLGEGTGACLGMDLVCAAIKI
                     YTEMATFDEAGVSDKA"
     gene            1292..2056
                     /locus_tag="VN647_09780"
     CDS             1292..2056
                     /locus_tag="VN647_09780"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HWG97361.1"
                     /translation="MGTVARPFIFAWHFLTTIPLSRTHHEPTAPELAASMAWYPIVGL
                     LIGGGLIVADFGLTAILAPAVVNVLLIALLVLVTRGLHQDGLADTLDGLAGGRIPAER
                     LSIMRDPRIGAIGATGLFLSLILRYAGLMVLPHELRLPAVLCMPAMGRWAMVTSAWIS
                     PYARTEGGLAAPFLTHLSWRHIALSTVVLTIALTAGFGAVGACLILLAGTLIVLVGWR
                     ACRRWFGGITGDTLGAANEVIEILFLLFVPLLLRLS"
     gene            2053..3009
                     /gene="cbiB"
                     /locus_tag="VN647_09785"
     CDS             2053..3009
                     /gene="cbiB"
                     /locus_tag="VN647_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HWG97362.1"
                     /translation="MTGGELLIASALDAMCGDPRWLPHPVRVMGRCITWLDHRVRMIC
                     RSRTSLRFAGICLAGGLPIMTYCIGVVMIEEAERFAAWLGSVLSIGLASTTLAARDLW
                     DHVRAVNGPLGVGDLPSARRAVAMIVGRDTAELSESEVARATVETVAESTADGLIAPL
                     FYLAIGGAPLALAYKAVNTLDSMIGHQDERYADFGWASARLDDLTNWIPARIAAVLLI
                     VGAGLTTGQLEQARNGWRVFRRDRSKHPSPNSGRPEAAMAGILGVKLGGTNSYDGIAQ
                     ERPVLGQEGRDAGPGDIMAAAKIMAGASVLGVSLAVGFRCLA"
     gene            2997..4106
                     /gene="cobD"
                     /locus_tag="VN647_09790"
     CDS             2997..4106
                     /gene="cobD"
                     /locus_tag="VN647_09790"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HWG97363.1"
                     /translation="MPRLKPRLHGGDIYGAARELNRDPAEILDFSASINPLGPSSQVW
                     KAITASRHLLSHYPDPHCWDLQQALATFWHADPEQIVVGNGSTELIDVLPRALGIHRL
                     LVVQPTFSEYAAAIERAGGQAMALYANRNDQYALPIDRLYRVMETGRIDGRSIDGIVI
                     CHPNSPTGQVCSADDVARLASIAKRRSLWLVIDEAFADYCPERSVLPHASSWPQVVVL
                     RSMTKFYALPGLRVGYAVATRAVIRRLRRQLPPWSISVMGQIAALAALNDAAHARRSL
                     QFMARERERLKHMLAALPGCVVMPTWTNYFLVELPRGRHARDVTEQMRNEGLLIRDCS
                     SIPGCNARSIRLAVRSQQENDRLIQALSNLLHDGV"
     gene            4109..4831
                     /locus_tag="VN647_09795"
     CDS             4109..4831
                     /locus_tag="VN647_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HWG97364.1"
                     /translation="MKGPATRVHTRHQVIGRTLVITLGGRRRILSSAPQGGGFRSASH
                     IINHQIAAHPGMNGPLPDPARYLRKLALELSIGYHTVGLMTAVPMTQLVTARVAAGEL
                     WVECFATVGVTNAVCAGEWPSSLAHRKEIYSTRVGTINLILITNGSLSTSAMVGAVQV
                     ATETKTGVLRDHTIPSWKGRDLATGTGTDAVVVACRVRGEGPLHTYSGTHTMMGALVG
                     RAVSHCVSRGLAKARKWQEMHR"
     gene            4828..6369
                     /locus_tag="VN647_09800"
     CDS             4828..6369
                     /locus_tag="VN647_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWG97365.1"
                     /translation="MKARALAILGTGSDVGKSLITAGICRLLRRTGVHVAPFKAQNMS
                     LNSFVTPGGHEIGRAQALQAEACGIPPHVDMNPILLKPESDNCSQVVVHGKVWGKREA
                     SAYFDGRQGLWSVVKESYARLAGQYEVIVIEGAGSAAEVNLREHDLVNWRVTELADAQ
                     VVLVADIDRGGVFAQVLGTLDLLLPDERSRVCGVIINKFRGDAKLFADGVKFLEARGG
                     LPVLGVIPFLHDLILDQEDSLDLGGNRHTSFASDRLNIGVILLPHMSNFTDFNILASE
                     PDVALKYVTSPFTLAEADVVIIPGSKNTLADLSYLKEKGYVTALNDHLHRQRELIGIC
                     GGYQMLGRTISDPCKVEQGGISGGLGYLNTETELKQVKCTTQVEASTEDVFVGGQILV
                     RGYQIHMGITRRESERHCFRVRRRSVLGEDVCLSIAASEEVFDGAIRKDGLVWGTYIH
                     GVFDEPGFRRAWLNRARMRKGVPPLDELVSKKVTVRSQGELDRWADHLSHNLDLSRLI
                     GYVRT"
BASE COUNT         1298 a         1707 c         2001 g         1469 t
ORIGIN      
        1 tcggtcgttc tcgaccgggt tggcatactt ttgcaaatga tgcagattgc ggcggccagg
       61 gtggtcatcg tcagcaatga gttaggattc ggtctggttc cgactgagcc gtcgacacga
      121 gcatttcgtg atcttgccgg acgggtgaat cagcagatcg ccgccggcgc agatgaagta
      181 tacctagttg tcagcgggtt accgcttcgc ctgaaatgaa tagaggagcc gtcatgccga
      241 tacaggacct gtgccgtctg attcaacctc tcaatgcgag tctgcacggg aaagcgcaag
      301 cgcgattgga tcgactgacc aagccgctcg gtagtctcgg gcgtctcgaa gaattggcgg
      361 cgtcttacgt cgctatcacc ggcgaactga agccgaatgt tccacgcgct gtcgtattta
      421 cctttgccgc cgatcatgga gtggctctgg aaggggtgag cgcttatcct cgggaagtca
      481 cgcctcaaat ggtgctgaat tttctacggg gtggagcagg agtgaatgtg ttggcccggc
      541 acgccggtgt ggatgtgcga gtggtggata tcggcgtcga ttacgagttc ggcgccgtgc
      601 ccggtcttct cgatcgaaaa gtcatgaaag gcacacgcaa tctggcggtt gaatcggcga
      661 tgacacgcag ccaagcagag caggccgtca tggtcggagt tgaactggct acagaagccg
      721 tgcgggaagg tatcggcctg atcggcaccg gtgagatggg tatcggtaat acgacaccaa
      781 gcgccgccat tacggcggtg atgaccggtc ggcctgcgga agaggtaact ggacggggga
      841 ccggcgtcga cgagtccggc cgtatgcaca aggcggcggt gatacagcga gcgctggaac
      901 tccatcgtct caatccgaca gacccactcg atgtcttggc gaaagtcggg ggactggaaa
      961 tcggtggatt ggccggcctg atgctcggcg cagcagcggt tcgggtgcct gtcgtgttgg
     1021 acggcttcat tgccggagcc gcggcgctga ttgccgtcgg gcttcagcct ttgtgtcgtg
     1081 agtatctcat tgcctcccat cgttcggttg agcaggggca tcgcgcggtc ttggaccatc
     1141 tgagactaaa accgctgttg gatctcgact tgcgtcttgg cgaagggacg ggtgcctgct
     1201 tgggaatgga tctggtttgt gccgcaatca agatttacac ggaaatggca acgttcgacg
     1261 aagcaggcgt gtccgacaag gcgtaacagg tatgggaacc gtggctcgtc catttatctt
     1321 tgcctggcat tttttaacga cgatcccctt aagccgaact catcatgaac ccaccgcacc
     1381 ggagctggcc gcctcgatgg catggtatcc gatcgtcggt ttgctgatcg gcggcgggct
     1441 catcgtggcc gactttggac tgaccgcgat attggcgcca gccgtcgtga atgtgctctt
     1501 gatcgcgctg ttggtgttag tgacacgtgg gcttcatcag gacgggttgg ccgacacatt
     1561 ggatggattg gcgggtggcc gtataccggc ggaacggctt tcgatcatgc gcgatccgcg
     1621 catcggagcg atcggcgcga caggcctctt cctctcgctg attctccggt acgccgggtt
     1681 gatggtgctg ccgcatgagt tgcggttgcc cgccgtgctc tgcatgccag caatgggacg
     1741 ttgggcgatg gtcacgtcgg cttggatttc gccttatgcc agaacagaag gaggattggc
     1801 ggcgccgttc ctcacccatc tgtcgtggcg gcacatcgcc ctgtccacgg tggtcttgac
     1861 gatcgcgctg acggcaggat tcggagcggt cggtgcatgt ctgatcctct tggccgggac
     1921 tctgatcgtc cttgtcggct ggagagcctg ccgccgctgg ttcggcggga tcaccggaga
     1981 tacgcttggg gctgccaacg aagtgatcga gattctcttc ctgctgttcg ttcctctctt
     2041 gctccggtta tcatgaccgg cggcgaactc ctcattgctt ctgctcttga tgcgatgtgt
     2101 ggcgatcctc gctggctgcc gcatccagtc cgtgtgatgg ggcggtgcat tacatggctc
     2161 gatcaccgag tacgaatgat ctgccggagt aggacgagcc ttcgcttcgc gggaatctgt
     2221 ctggccggag gattgccgat catgacttat tgtatcggcg ttgtcatgat tgaagaggcc
     2281 gaacgtttcg cagcatggct ggggtcggtc ctgtcgatcg gattggcttc gacgacgctt
     2341 gctgcccgag atctgtggga tcatgtccgt gccgtgaatg gtccgctcgg cgtcggggat
     2401 ctcccatccg cacgccgagc tgtcgccatg attgtcggac gagacacggc ggagctttcc
     2461 gaatccgagg tagcccgcgc gactgtggaa acggtcgcgg aaagcacggc agacggcctg
     2521 atcgccccgc tgttttatct ggccatcggc ggcgctcctt tagccttggc ctataaggcc
     2581 gtcaatactc tggattcgat gatcggtcac caagacgagc gctatgcgga ttttggatgg
     2641 gcatcggccc gactcgacga tctgaccaac tggattccgg ctcgtattgc tgcagtcttg
     2701 ctcatcgtcg gcgcgggatt gaccacagga caactggaac aagcccgcaa tggatggcgg
     2761 gtgttccgtc gcgacaggag caaacatcct agtcccaaca gcgggagacc agaagcggcc
     2821 atggcaggga tcttaggagt gaaattggga ggcaccaact cctatgacgg catcgcacaa
     2881 gaacgtcctg ttctcggtca agaaggacgc gatgcaggac caggcgatat catggcggca
     2941 gcgaagatca tggcgggagc ctctgtgctc ggtgtctctc tagcggtggg tttcagatgc
     3001 ctcgcctgaa gccgcgcttg cacggcgggg atatctatgg tgccgcacga gaactgaacc
     3061 gtgatcccgc agagattctt gatttcagcg ccagcatcaa cccgttgggg ccttcctctc
     3121 aagtctggaa agccattacc gcgtccagac atctgctgag ccactatccg gatcctcact
     3181 gttgggatct gcagcaggcg ctggccacat tttggcatgc tgatccggaa cagatcgtgg
     3241 tcggtaacgg ttccacagaa ctgattgacg tgcttcctcg tgcattgggg atccatcggc
     3301 tcctcgtcgt ccagcctacc ttttctgaat atgctgctgc gatagagcga gccggaggac
     3361 aagccatggc gctctatgca aaccgaaacg accagtatgc gctcccgatt gaccgtctct
     3421 accgagtgat ggaaacagga cggatcgacg gccgctccat tgatggaatc gtcatctgtc
     3481 atcccaatag tccgacaggg caagtctgct cagccgatga tgtcgcgcgg ttagcgtcca
     3541 tcgccaagcg gcgaagtctt tggctggtga tcgatgaagc atttgccgac tattgtccag
     3601 aacgatctgt cctgccgcac gcgtcgtcct ggccgcaggt cgtcgtctta cgcagcatga
     3661 cgaaattcta cgccttgccg ggactacggg tgggatatgc ggtggcgacc cgtgcagtca
     3721 tcagacgctt gcgacggcaa ctgccacctt ggtcgatcag cgtcatggga cagatcgctg
     3781 cactcgctgc tttgaatgat gcggcacatg cgcgaagaag tctgcaattc atggcacgag
     3841 agcgcgaacg gttgaagcac atgcttgccg cactgcccgg ctgtgttgtg atgccgacat
     3901 ggacaaatta cttccttgtc gaattgccac gcggtcggca cgcgcgtgac gtgactgagc
     3961 agatgcgaaa tgaggggttg ttgatccgcg attgttcatc cattcccggg tgcaacgctc
     4021 gttcgatccg acttgccgtg cgatctcagc aagagaatga ccggctcatc caagccttgt
     4081 cgaatctgct tcacgacggt gtgtagtgat gaagggacct gctacacgcg tccacacccg
     4141 gcatcaagtg attggacgca cgctggtgat cactcttgga gggcggaggc gtattctttc
     4201 atctgcaccg caaggaggtg gattcaggtc cgcctcacac attatcaatc atcagatagc
     4261 ggcgcacccc ggtatgaatg gaccattgcc cgacccagca cgttacttgc gaaagctggc
     4321 tttggaactc agcatcggat accacactgt cgggctcatg acggcagtcc cgatgacaca
     4381 gcttgtcacc gctcgggttg cggcgggaga gctctgggta gagtgtttcg ccacggtcgg
     4441 ggtcacgaat gcggtttgtg ccggggagtg gccgtccagt ctcgctcatc gaaaggaaat
     4501 atattcgacg agagtcggaa cgatcaatct catccttatc acgaatggta gtctctcgac
     4561 ctccgccatg gtcggcgcgg ttcaggttgc cactgagaca aagacgggcg tcttgcgtga
     4621 ccacactata ccgagttgga aagggagaga ccttgcgact gggacgggta ccgatgcggt
     4681 ggtcgttgcg tgccgcgtgc gcggtgaggg accgctgcat acctacagcg ggacccacac
     4741 aatgatgggt gcgctggttg gaagagcggt ctctcattgt gtctccagag gcttagccaa
     4801 ggcgagaaag tggcaggaaa tgcaccgatg aaggcgcgag cgcttgcgat tctcggtacc
     4861 ggctcagatg taggaaagag tttgatcaca gccggaatct gtcgcctgct tcgtcggacc
     4921 ggcgtccatg tggcaccatt caaagctcaa aacatgtcgc tgaattcgtt cgtcactcct
     4981 ggcggtcatg aaatcggaag agctcaagct ctgcaggcag aggcatgcgg gattccgccg
     5041 catgttgaca tgaacccgat tctcctcaag ccggaatccg ataattgttc gcaggttgtg
     5101 gtccatggca aggtgtgggg gaaacgcgag gcatcggcgt acttcgacgg acgacaaggg
     5161 ctctggtctg tggtcaaaga aagttacgcc cggttggccg gccagtacga agtgatcgtg
     5221 attgaggggg caggcagtgc ggcggaagtc aacctccgtg agcatgatct ggtcaattgg
     5281 cgggttaccg aattggctga tgctcaggtc gtactggtgg ccgatattga tcggggcggg
     5341 gtttttgcgc aagttcttgg aaccttggat ttattgctac cagacgaacg ctccagagtc
     5401 tgcggggtca tcatcaataa attccgtggt gacgccaaac tattcgccga tggggtgaag
     5461 ttcttggaag cgcgaggggg gctgccggtg ttgggtgtta tcccgtttct tcatgatctg
     5521 atactcgatc aagaagacag tcttgatctc ggtgggaacc gacacacaag ttttgcgtcc
     5581 gaccgactca atattggcgt cattttactg ccgcatatga gcaactttac cgatttcaat
     5641 atcttggcgt cggaaccgga tgtcgcattg aagtatgtga cctcgccatt cacgcttgcc
     5701 gaggccgacg tcgtgataat tcccgggagc aaaaatacgc tggcagatct ttcctatttg
     5761 aaggagaagg gctatgtcac tgccctgaac gatcatctgc atcgtcagcg agaactgatc
     5821 ggcatctgcg gtggctatca aatgctcggc cgaacaatct cggacccttg caaggtcgag
     5881 cagggtggga taagtggcgg actgggttac ttgaataccg agacagaact caagcaagtc
     5941 aagtgcacca cacaagtgga agcaagcacg gaagatgtct tcgtgggagg tcagatcttg
     6001 gtccgtggat atcaaattca tatgggcatc acgcgacgcg agagcgaacg tcactgtttt
     6061 agagttcgcc gccgttcagt attgggtgaa gacgtttgcc tgtcgattgc cgcaagtgaa
     6121 gaagtcttcg atggagccat tcgaaaagac ggtcttgtgt ggggcacgta catccatgga
     6181 gtgtttgatg agcccggttt tcgtcgtgcc tggctcaatc gcgctcgtat gagaaaaggg
     6241 gttccgcctc ttgacgagct cgtatcaaag aaggtcaccg tacgatcaca gggcgaactt
     6301 gatcggtggg ctgatcacct ctctcataac cttgatttat cccgtctcat cggatatgtc
     6361 cgcacataaa ttccatcaac tgctggagta gccgcgttca gcattcgtct agcagtgtgt
     6421 tgacaaacct ctcctcggcc cgtcgtgctt gtgataagag aggcctccat cagga
//
LOCUS       DATNDD010000402         3488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4168900, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000402 DATNDD010000000
VERSION     DATNDD010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4168900"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="queC"
                     /locus_tag="VN647_09805"
     CDS             <1..721
                     /gene="queC"
                     /locus_tag="VN647_09805"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWG97366.1"
                     /translation="LHPPFHIMNGQTSERAVVLASGGLDSTVTAALARRDGYALYLLT
                     IAYQQRHAVEVERARQVATALGAQRHVVVDVDLRAIGGSALTGDMAVPKHRTESERNR
                     DVPVTYVPGRNLIFLSLAAAHAEVSEASIIYFGANVIDYSGYPDCRQEFINAVEATLQ
                     AGTKAGMQGKGIEIRAPLLRMTKAEIIKLGLILNVPFHLTHSCYDPIGPIACGQCDSC
                     LIRKRGFAEAGVVDPIQYAVC"
     gene            757..1152
                     /locus_tag="VN647_09810"
     CDS             757..1152
                     /locus_tag="VN647_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWG97367.1"
                     /translation="MKTSRMLWMSLVMLVLGAAPACSQNGVEPKGTGAVTSAPAELWE
                     GEQKFNANCSTCHGIGGVGTTQGPPFLHKVYEPNHHGDAAFQRAAANGVKAHHWQFGD
                     MPKIDAVKQDDVDQIIKYVRWLQKQAGIF"
     gene            complement(1224..1580)
                     /locus_tag="VN647_09815"
     CDS             complement(1224..1580)
                     /locus_tag="VN647_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97368.1"
                     /translation="MRIISMVLGVTVLTVMIGMGLSTLALSGTDRTVYDKPDIRSGKI
                     IRGDVMKVDEKSSQSWNVSVKDRETGEVVVLLIDKSTTRKSISLRPDLGDYVIAQYNE
                     HNNHAYSFLTNEREHN"
     gene            complement(1775..2239)
                     /locus_tag="VN647_09820"
     CDS             complement(1775..2239)
                     /locus_tag="VN647_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG97369.1"
                     /translation="MNEVLKRAQTGDPNAQNELGLLYSEGRGLPQNYREAKDWFKKAA
                     DQGHAGAQVNLGTLYSLGRGAPYSDHMALFWFQKAAEQRNALAFAKLGMMYERGRAVP
                     QSLIEAHMWYNLSVAYGEKRAAESREAVAIRMTPGQIAEAEDRAKKWMPTRR"
     gene            complement(2634..3221)
                     /locus_tag="VN647_09825"
     CDS             complement(2634..3221)
                     /locus_tag="VN647_09825"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG97370.1"
                     /translation="MGGCLRNFIMITVICTLSACIDPPRSPYVDVRDMVSRIDELQST
                     AEQGSAEDQYNLGMRYEKALPKDHREAVRWYRMAATQQHVGAFYRLCVLSDIGHGMPQ
                     DYQEALRWCRLAAGQGHGPAMFLIATYYVKARGVPKDVVQAYRWYNLAAANGHEDGVK
                     WRDRLALNMTPTQIAQAQFLARNWKPKVYHSVQEQ"
BASE COUNT          782 a          899 c          936 g          871 t
ORIGIN      
        1 cctgcatcct cctttccaca tcatgaatgg gcagacttca gaacgagcgg tcgttcttgc
       61 cagtggtggg ttggactcca cggttacggc ggcccttgcg cggcgtgacg ggtacgcgct
      121 gtatcttctc accattgcat accagcagcg tcacgctgtg gaggttgagc gggcaaggca
      181 ggtcgcgacc gctctggggg ctcagcggca tgtagtggtg gacgtcgatt tgagggcgat
      241 cggtggatcg gccttgaccg gcgatatggc ggtgccgaag catcggactg aatccgaacg
      301 gaaccgggat gtgcccgtta cctatgtgcc gggccgaaac ctgatttttc tgtctctggc
      361 tgcggctcat gcggaagtat cggaagcctc gatcatctat ttcggagcga atgtgatcga
      421 ctactcgggc tatcctgact gccgccagga attcattaac gccgtggaag caacgcttca
      481 agcgggaaca aaggcaggca tgcagggcaa aggcattgag attcgagcgc cgctgctccg
      541 gatgaccaag gcggagatta taaagctcgg gctgattctg aatgtcccct ttcatctcac
      601 gcatagttgt tatgacccaa ttgggccgat tgcctgcggg cagtgcgaca gttgcctgat
      661 tcggaaacga ggatttgcgg aggcaggagt tgtagatccg attcagtatg cggtatgttg
      721 agccaacggt ccgattccga taacgatcac acttctatga agacatcacg aatgctgtgg
      781 atgagtctcg ttatgttggt ccttggtgcc gcaccggcat gcagccaaaa cggggtggaa
      841 ccaaagggaa cgggagccgt gacctctgcg ccggcggagt tatgggaagg agagcagaaa
      901 ttcaacgcca actgttcaac ctgccacggg atcggcgggg tcggaacgac tcaaggtcca
      961 ccgtttcttc acaaagtcta tgagccgaac catcacgggg atgcggcgtt tcaacgtgcg
     1021 gcggccaatg gagtaaaggc tcatcattgg cagtttggtg acatgccgaa gatcgatgcg
     1081 gttaaacagg atgacgtgga tcaaattata aaatatgtcc gctggctcca gaaacaagcc
     1141 ggcatttttt aaacctttat ccctctttat accttggcct atttcccttc ctgatctact
     1201 gatcgttcgc ggtgaacacc tcgtcagtta tgctcgcgct cattcgttaa gaatgaatac
     1261 gcatgattat tgtgttcatt atattgtgcg ataacgtaat ctcccaagtc gggtctaagc
     1321 gagatgcttt ttctcgtggt actcttgtcg atgaggagaa ccacgacttc gcctgtttcc
     1381 cggtctttta ccgacacgtt ccaggactgt gaggattttt cgtcgacttt catcacatcc
     1441 cctcggatga tcttgcctga ccgtatatca ggtttgtcat agaccgtcct atccgtgcca
     1501 gaaagggcga gtgtagacag gcccatacca atcataactg ttaaaactgt cactcctaat
     1561 accattgata taatacgcat aatctaattc tcctctcgta ttctctttag tacatttact
     1621 tcatgtgagt ggcattggaa gctggaagaa tcactagttt ttgatttgaa agcttgtgca
     1681 cgtcagagtt tactaaggaa gtaacgagcc gcttctcatt accgacccga ccacctcact
     1741 ctccgacagg cattgggttc cgaagagcat gttgtcatcg tcgcgtgggc atccacttct
     1801 tcgcccgatc ttccgcttcg gcaatctgtc ccggcgtcat ccgtatggca accgcctcgc
     1861 gagattctgc ggcacgtttc tcaccgtagg caacagaaag gttgtaccac atgtgggctt
     1921 caataaggct ctggggcacg gcgcgtcctc gctcgtacat cattcccagc ttcgcgaaag
     1981 ccaacgcatt tcgttgctcg gcggcctttt gaaaccagaa caacgccatg tggtcactat
     2041 acggtgcgcc ccgtccaaga gaataaagag tcccaaggtt gacctgtgcc cctgcgtgcc
     2101 cttggtcagc ggctttttta aaccaatcct ttgcttcacg atagttttgt ggaagacctc
     2161 gcccttcgct atacagtaaa cccaattcat tttgagcatt gggatcgccg gtctgagcgc
     2221 gtttgaggac ctcgttcacg gccggtccct caatgagcgg ttgtggcgga ctaggattgg
     2281 gagcggcgca gcttgacacg aaaaaagaca gtaagagaaa tcctacgaga gtcccacaaa
     2341 aggagagtgg cagccggtga gccattctga atgattgtga acgtgacgaa tatctgctgg
     2401 tcggtcagcg aaccataggc aaatatccta cgcagcgaga aggcgcgact tcaagcacga
     2461 ttgtaatgtt gcaatagcgg aggaggttga tggagccatg agatgtcacc ggtctcccga
     2521 gacttgttgc gcgcatggct tcgtagttgt cgacaggaat gaaaaggctt caaggagtga
     2581 atccaggttg ctggtcttcc gatatagagt atgcgtctgg agaggtctgt aggctactgt
     2641 tcttgaactg aatggtagac cttgggtttc cagttccgtg cgagaaattg tgcctgtgcg
     2701 atttgagtcg gcgtcatgtt caaggccaga cgatctcgcc atttcactcc gtcctcatgg
     2761 ccgttagccg ccgccaagtt gtaccaccgg taggcttgga cgacatcctt gggaactccc
     2821 cgtgccttta cataataggt ggcgatcagg aacatcgctg gcccatgccc ctgacccgcg
     2881 gcaaggcggc accaccgtag agcttcctga taatcctgag gcattccatg accgatgtcc
     2941 gagagcacgc agagccgata gaatgcaccg acatgttgct gggtagccgc catgcggtac
     3001 caccggacag cttctctgtg atctttcggc aaggctttct catatcgcat gccaagattg
     3061 tactgatcct cggcgcttcc ttgttctgcg gttgactgta attcatcgat ccgcgacacc
     3121 atgtcccgca cgtcgacgta aggagacctt ggtggatcaa tgcatgccga cagggtgcag
     3181 atgacggtta tcatgatgaa atttcgcagg caacccccca taatcccttt cctccgaaat
     3241 gatcccgtgt cagctgtctt ggtggttcac atccgacaaa gagcggatgc agagtctact
     3301 ctagcacatc tcgctctctg gaaagccagc atggtcacga tcttgttcac gccctgttgc
     3361 tttcactgaa gagaaaagat atctgttagg tatggtacca aggtgaaatc ttggctcttg
     3421 ttactgtgcg gaagatcatg agaagcacta tccttgtggg aatcttattc cttctgtccg
     3481 cattatcg
//
LOCUS       DATNDD010000403         5911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1048557, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000403 DATNDD010000000
VERSION     DATNDD010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048557"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VN647_09830"
     CDS             complement(<1..1380)
                     /locus_tag="VN647_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWG97371.1"
                     /translation="MSLELAKKLYAKMPEIFEKARKKFGRPLTLAEKILVSHADNFDA
                     QHWERGKAMLALRPDRVAMQDATAQMAMLQFMQAGKKKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGGLIIGTDSHT
                     PNGGGLGMLAIGVGGADAGEVMAGLPWEVLHPKLIGVKLTGKLSGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDQKMVDYLNITD
                     RADLAKLAQANKVLLIADPEVNQSPEKYYDQIVEIDLSKLEPHVVGPHTPDLARPVSK
                     VAAEAKEKGYPVELKAALIGSCTNSSYEDISRSAHIARQGLKAGLKAKTSFLVSPGSE
                     RIYHTMKRDGFLDTFEKLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGINETLSF"
     gene            complement(1623..3443)
                     /locus_tag="VN647_09835"
     CDS             complement(1623..3443)
                     /locus_tag="VN647_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWG97372.1"
                     /translation="MSILANKDTRVVVQGGAAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDVGKTNEIPYGSGLIAIPIYKTIAEATKYHPTINTSLVYIGADRAMKGGMEALDDP
                     HIKVVSMITEGVPEKDAKLLGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVITKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRVKLIGVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKSGAIVPPTFGALGPAIKDAYQELLKSGQVKEPVEPAVLP
                     RLPKSIEEAMKADEVMVAPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQAVAAGLIMIGPRF
                     GGAVTDAGRWFKYAVDNKMSVDEFLAYMKKNVGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLHIKTPCLDFALAVEQVTAVKKDNLILNVDGTMAAVLVDLGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            complement(3526..4725)
                     /locus_tag="VN647_09840"
     CDS             complement(3526..4725)
                     /locus_tag="VN647_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HWG97373.1"
                     /translation="MAKVLEGPGMGLMKKWGIHVPHYAVVTSAEELSKLGHVNEWMKQ
                     TKLVVKAHEALGSRFKLGLVKVGLDLNGAVAATKEMIGRQVGSITISQVIVSEMVPHK
                     DEYYCAVKSTREGAEILVANCGGIEVESNWERVKRLCLDVGQAPSPEQLEKLAKDAGF
                     TGSVAKKMADFAGKMFACFDNEDAQYLEVNPVVTRESDGELIALDAGTLLDGDAKFRH
                     PDWNFQFAAEFGRAYSKDEVAVMAVDGKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIKNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKGDGKLKGVKIWVRRGGPREKEGLDAMRALKDEGFDIHVFDRNTPLTDIV
                     DKALQKQ"
     gene            complement(4906..5745)
                     /gene="mutM"
                     /locus_tag="VN647_09845"
     CDS             complement(4906..5745)
                     /gene="mutM"
                     /locus_tag="VN647_09845"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWG97374.1"
                     /translation="MPELPEAEVVARQIRTRLLGARLREIWVGRTDIVREGYTTVSWY
                     QGATLQSVDRYGKSIALGFINDLDGRYVVAELGMTGLLLFQSAQVKSPQHIHVRLLFD
                     GSSEPELRYWNPRRFGRLSLLDKTGLERYLARRFGLDPLLVSQPDFVRVLRERRGRLK
                     SLLMHQQVVAGIGNIYANEILFRAGLHPNTPVGRLSVGKMEKLHRIMREVLEEAIACG
                     GSSVRDFFAPDGTEGQYKRRHLVYGKGGQPCPNRCGGIIRRFQTERSAYFCPRCQSLR
                     PTT"
BASE COUNT         1252 a         1801 c         1560 g         1298 t
ORIGIN      
        1 aaacgacagt gtttcattta tgccgtcatt gcggcctgga aagttccggt tgaaggaact
       61 gacgatcgaa tccgccttcc ctttgacacc gtcggcccgt ttccactgcc cgatgcaggg
      121 cccgcacgag ttcgacagca cggtaccgcc gagcttctcg aacgtgtcca ggaatccgtc
      181 ccgtttcatc gtatgataga tgcgctcgga gccgggtgaa acgaggaacg acgtcttggc
      241 tttcaacccg gccttcaacc cctgccgcgc aatgtgcgct gatcggctga tgtcttcata
      301 cgacgaattg gtgcagctcc cgatcagcgc cgccttcaat tcgaccggat agcccttctc
      361 cttcgcctcg gccgcaacct tggaaacagg cctggccaaa tcgggagtat gggggccgac
      421 cacatgcggt tcaagtttgg ataggtcgat ttcgacgatc tggtcataat acttttcggg
      481 cgactgattt acttcgggat cggctatcaa caacaccttg tttgcttggg ccaatttcgc
      541 caaatccgcc cgatcggtga tgttcaggta gtcgaccatc ttctgatcga agggaaatac
      601 cgaggtggtc gcccccagtt ccgcgcccat attgcagata gtgcctttcc cggttgcgct
      661 gatcgtctcg gcgccgggac cgaagtattc gacgatcttg ttcgtcccac ccttgacggt
      721 gagcaggccg caaagataga ggatcacatc cttcggagac gcccaaccgc tcaacttgcc
      781 ggtcagtttc acgccgatca acttcggatg gagcacctcc cacgggagac cggccatgac
      841 ttctccggca tccgctcctc cgacaccgat ggccaacata cccagaccgc cgccgttcgg
      901 tgtatgagag tcggtgccga tgatcaagcc gccaggaaat gcataattct ccagcaccac
      961 ctggtgaatg atgccggcac ccggcttcca aaagccgatg ccgtacttct tcgccgcgga
     1021 agccaggaag ttgtaaactt ccttgttttc atccatggcg cgcaggagat ctttttcaga
     1081 tcccatctcg gcacggatca agtggtcgca gtggatcgtg ctcggcacag cggctttctt
     1141 cttgccggcc tgcatgaact gcaacatggc catttgcgcc gtggcatctt gcatcgccac
     1201 gcggtcggga cggagggcca acatggcctt cccacgctcc caatgttggg catcgaagtt
     1261 gtcggcatgt gaaacgagaa tcttttccgc caacgtcaac ggacggccga atttctttct
     1321 ggccttctcg aagatctccg gcattttggc gtagagcttc ttcgcgagct caagtgacat
     1381 tgcgctctcc ttacaaacag gcttggaact tggagccagg agccaaggag ttgtactccc
     1441 cggccacgcc ccttcaccag ccttcggtta gggcggcatg ttccgtcact tgccaagcga
     1501 cggaacctcc cgcaattatt tcaatggtgg cgcttcacga gtctatttca atgacgggac
     1561 ttcccgcgtc tatttcaacg acggaacctc acgcgacatt ttagcggggg gacctcccgc
     1621 ctctacttta acggcggcac ttcgcgccgt ttgggcgccg catagttcac caggtaatcg
     1681 aacaaccgga tcaagcggct gtcctggcgc ttctgatcga cccaatggcc gatcaatccg
     1741 atcgtgcgag acaggatgaa gaagccgttc aaactgtcga ccgggaatcc cagatcgacc
     1801 agcacggccg ccatcgtgcc gtccacgttc agaatcaaat tatccttctt cactgccgtc
     1861 acctgctcca ccgccaaggc gaagtcgaga cagggcgttt tgatatgcaa gctcttcacg
     1921 taacccacga gctccttcac gcgcttgtca ggattgcgca agctcttcac tcggtggccg
     1981 ataccaggta caggtcccac attctttttc atgtaggcca ggaactcgtc cacactcatc
     2041 ttgttgtcga ccgcgtactt aaaccagcga ccggcgtccg tgactgctcc gccgaagcga
     2101 ggtccgatca tgatcaagcc ggctgccacc gcttgggaca gcccgatccc ggcacaggcc
     2161 gcaagaatcg tcgcgtaggc gccgctgacg caggggccgt ggtcggcgga gagcatcatg
     2221 atgcgcttga tgatttccgc ttcctgctta gagatcagcc gcttgtccca gagaagaccg
     2281 atgacatggg gaatttcata gcccttgttg atgagctcgg aggccggata accgtcgtag
     2341 caaggttcgt cccctcgatc gtcgctgatc gtggtacgga tgagcggtgc gaccatcact
     2401 tcgtcggcct tcatcgcttc ttcgatggac ttgggcaacc gcggcaggac agccggttcg
     2461 accggctcct tgacctgacc ggacttgagc aattcttgat aggcgtcctt gatcgcggga
     2521 ccgagcgcac cgaatgtcgg cgggacgatc gcaccggatt tcttgagcgc gtcggacttc
     2581 gagcgggccg aaccttcgcc cttcatccct tccttggcgc cggcatggcc gaacttcatc
     2641 cccttgggca aactctcctg acagaatccg gagacgacgc cgatgagctt cacccggcgc
     2701 tttttcgcgc cataccactc ggcggcccgc tcttcgagat caccgcccat ttcaccgacg
     2761 atgacgaccg ccttcgtctg cggatcattc tcgaacattt cgaggtagct cacatagtcg
     2821 gtgccgggat aagcatcgcc gccgatgccg atggcggtcg tgatgccgtc ggcgaactgc
     2881 gaacaaatcc aaatgatttc gttggaaaga ccgcccgact tggtaatgac gccgaaggaa
     2941 ccttcccgat agagcttgga gaggacaaga ttgtcgaagg ccccgccgat cacgccgagc
     3001 cggcaagcgc cggcggaaat aataccgatt gaagaggggc cgttgaatac cttgccgagc
     3061 ttacgcgcat gggcgccgag caatttcgca tccttctccg gcacaccctc ggtgatcatc
     3121 gagaccacct taatatgtgg atcgtccagc gcttccatcc cgcccttcat ggcacgatcc
     3181 gcgccgatat agacaaggct cgtattgatg gtcggatgat acttcgtcgc ttcagctatt
     3241 gtcttgtaga tggggatcgc aatcaaaccg ctgccgtagg gaatctcatt ggtcttgccg
     3301 acgtcgggcg ggtaaacgaa cgcctcaacg ttgagtgggc gcttgatcag ataacagaac
     3361 tccgccatgc gccgggcggc gttgacacct gccgcacccc cctgaaccac cacgcgggtg
     3421 tctttattcg ccagaatact catcgcaatc tcctctttcc ttcaggactg agtgctgagg
     3481 acggagagac gaggaaaggc ccactcactc agtcctcagt cctcgttact gtttttgaag
     3541 tgctttgtcg acgatgtccg tcagcggggt gttgcggtcg aagacgtgga tgtcgaagcc
     3601 ctcgtccttc agcgcgcgca tcgcgtcaag cccttccttt tcacgaggcc caccgcggcg
     3661 cacccaaatc ttgacaccct tcaacttgcc gtcgcccttt gccttgcgga atccattgat
     3721 gatgccgccg aaggtcttct tcacgtcggt aaaattggcg atcgccccgc cgacgatgat
     3781 attcttgata ccgggcaacg agcagacctt ttccgtcagc acttcaaccg cccagtcggg
     3841 gggatcaccc gaatactcgg catagttcgc caacttgccg cctcgcgcca cgacggcgtc
     3901 tgagtagtag acactcgcgc cgccgcctgc cggaagcatc gccgtatcgc cgcctggaat
     3961 ttcgatgaat ttgacggacc ccttgatctt gccgtcaacc gccatgactg ccacttcatc
     4021 tttgctgtag gcacgcccaa attccgcggc aaactggaaa ttccaatccg ggtgacggaa
     4081 tttggcatcg ccgtccagca gcgtcccggc atcgagtgca atcaactcac cgtcgctctc
     4141 gcgtgtcacg accggattta cttcgagata ctgcgcatct tcattgtcga aacaggcaaa
     4201 catctttccg gcgaagtcgg ccatcttctt ggcgaccgag ccggtaaatc ctgcatcttt
     4261 ggcgagcttt tcaagttgct ccggggaagg agcttgcccg acatcgagac acaaccgctt
     4321 cacgcgctcc caattggact ccacttcgat accgccgcaa ttagccacaa ggatctcagc
     4381 accttcacga gtggacttca ccgcgcaata gtactcgtct ttgtggggga ccatctccga
     4441 gacaatgacc tgcgagatgg taatgctgcc gacctggcgg ccgatcattt ctttggttgc
     4501 cgccacggca ccattcagat cgaggccgac cttcaccaga ccgagcttaa accgggaccc
     4561 cagcgcttca tgcgctttaa cgaccagttt cgtctgtttc atccactcgt tgacgtgacc
     4621 aagcttggag agttcctccg cggaggtgac aaccgcataa tgtggaacgt gaattcccca
     4681 cttcttcatc agtcccatcc cgggaccttc caaaaccttt gccatgagcg ttctccttcg
     4741 tattcgactc ggtctctagg gggagccgga ttgtaacgga aatcaacggg atgactcaag
     4801 acaccagagg gactacgatg aagcggatca aaaggcctag cgtgaaattt tcaaacctag
     4861 cttttactaa gcagcgaaaa tgtaagtgct ttttcccttc cgacattacg ttgtaggccg
     4921 aagagattga catcgcggac aaaaatatgc gcttcgttcc gtttgaaacc gtcgaatgat
     4981 cccgccacag cgattgggac aaggttgccc tcctttccca tagaccagat gccgccgctt
     5041 gtactgtccc tctgttccat cgggggcgaa aaaatctcgg acgctcgagc ctccgcaggc
     5101 aatcgcctcc tcgagaacct ctcgcatgat ccgatgaagc ttttccattt ttcccactga
     5161 aagccgtccg accggcgtgt ttgggtgtaa cccggctcta aagagaattt cattcgcgta
     5221 gatgtttccg ataccggcaa cgacctgttg atgcatcaag agagatttga gccggccacg
     5281 tcgttctctc agaactcgca caaaatcagg ttgcgacacg agaagtggat cgagcccgaa
     5341 tctgcgagcg aggtatcgct caaggcccgt cttgtccagc agggaaagac ggccgaagcg
     5401 acgagggttc cagtaccgta gttccggttc actggagccg tcgaacagca gccgtacatg
     5461 gatgtgttgc ggggatttca cctgcgcgga ctggaacaga aggagtcccg tcatcccgag
     5521 ctcagccact acatatcgac cgtccagatc attgatgaat cctagggcaa tgctcttgcc
     5581 gtatcgatca acagactgca gagtggcgcc ttgataccaa gacacggtgg tatagccctc
     5641 tcgaacgata tctgtacgac cgacccatat ctctctcagc cgtgccccga gaagacgagt
     5701 ccggatctgt cgggcgacaa cctctgcttc tggaagctcc ggcatacctc gaccatgcta
     5761 agagatgaag agaaatccta ttacgccatg gagattgcgg aaaggcaagg tccgcggcgc
     5821 aaagaataat gaggcgcacg gcgcgatgaa gaaagaaagc cacgtctgtg cgcgccggcg
     5881 aggtccacgc catgccgaat aaggcatggc a
//
LOCUS       DATNDD010000404         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3534883, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000404 DATNDD010000000
VERSION     DATNDD010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3534883"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VN647_09850"
     CDS             <1..425
                     /locus_tag="VN647_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWG97375.1"
                     /translation="EFNVTVHGETYHVKVSGSGRKIDGRKPYYIRVTDKLEEVSLEPI
                     QEVLAGVPETQESESGGKPKRPRPSKPGDVAPPMPGRVVKILVAVDDRVKAGDPLLII
                     EAMKMESQVPAPIDGRVAMILAAEGDNVKTDETVIQLE"
     gene            472..1410
                     /locus_tag="VN647_09855"
     CDS             472..1410
                     /locus_tag="VN647_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWG97376.1"
                     /translation="MPLILCLVSYLMTACAPPSQIPPYFETLERTPIERIPIKTVLVH
                     DQRIAYLDIGTGPPVILIHGFGGSMWQWEHQQHALSQHFRTLTLDLPGSGLSDKPDID
                     YLPDQMLDFCIGFMDALEIPQVTLVGNSMGAGLAIGMALTHPTRIDKLVLIDGLPSHV
                     KAKLTSRSFRQALESRAPSWLVSFGNWLFGGLVTESVLKEIVHDHRLLTPAVIERSNR
                     NRRRPGIIKPIMAVRNALPSWEADFAPRLSSITHPTMIIWGEYDRLFPVAVGEELHHR
                     IRGSTFVRVSDAGHMPQWERPDLVNRSLIAYIQPSP"
     gene            1462..2127
                     /gene="tpx"
                     /locus_tag="VN647_09860"
     CDS             1462..2127
                     /gene="tpx"
                     /locus_tag="VN647_09860"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HWG97377.1"
                     /translation="MRTRYSAFIGTALCFSLSLVGCQTMKGSTGAGFSYKNISVADGS
                     AVAGDGNKVLFKGNPLALTGNGVKVGDQLRDVKVAQNDLSLVNIAQTKGAGKVRVISV
                     VPSLDTPVCEQQTHHLSERSKGLDKMIELITVSVDTPFAQKRFAEEANIANITFLSDY
                     RGAEFGKTHGLFLEGPNILTRAVMVVDKTNTIRYLQITPELSQLPDMEEALQFARRLI
                     TES"
BASE COUNT          501 a          636 c          595 g          477 t
ORIGIN      
        1 tagaatttaa tgtgaccgtg catggagaga cctaccatgt gaaggtctcg ggctccggtc
       61 gaaagatcga cggccgtaag ccctactaca tccgtgtgac cgataaactc gaagaagtgt
      121 cgctcgaacc catccaggaa gtgctcgccg gtgtcccgga gacccaggag agtgaatcgg
      181 gaggaaaacc caaacgtccc agaccttcga aacccggtga tgtggctccg cccatgccgg
      241 gccgagtggt gaaaatcctg gtggcggtcg acgatcgcgt caaggccggc gatcctctgt
      301 tgatcatcga agcgatgaag atggaaagcc aggtgcctgc gccgatcgat gggagggtcg
      361 ccatgatttt ggctgccgag ggtgacaatg tgaagacgga tgaaacggtc attcagctgg
      421 aatagttcca tcaccggctc aatcccgcgc tcttgccgaa cgaggcggct tgtgccgctc
      481 atattgtgcc tcgtatctta tctcatgacc gcctgtgcgc cgccttcaca gatcccgccc
      541 tattttgaaa cattggaacg tactccgata gaacgcattc cgatcaaaac tgttctcgtc
      601 catgatcaac ggatcgcgta tctcgatatc ggtaccggcc ccccggttat tttgatccat
      661 ggcttcggcg gatccatgtg gcaatgggaa catcagcaac atgcgttgtc tcaacacttc
      721 cgcacgctga cgctcgacct gcccggctcc ggattgtccg acaagccgga cattgattac
      781 ctgccggatc agatgttgga cttctgtatc ggattcatgg acgccctgga gattcctcaa
      841 gtcacattgg tcggcaattc catgggcgct gggctggcta taggaatggc attgacccat
      901 ccgacccgca tcgacaaact cgtgctcatc gacgggttgc cgtctcatgt caaggccaag
      961 ctcaccagcc gctcgttcag gcaagccttg gagtctagag ctccttcctg gctcgtctcc
     1021 ttcggcaact ggctgttcgg cgggctggtc accgagtcgg tcctcaagga aatcgtccat
     1081 gaccaccgcc tcctcacgcc ggccgttatc gaacggtcca accgcaatcg ccgacgcccc
     1141 ggcatcatca aacccatcat ggccgtgaga aacgccctcc cctcctggga agccgatttc
     1201 gctccacgcc tcagcagcat cacgcacccg accatgatca tctgggggga atacgaccgg
     1261 ttgtttcccg tggctgtggg tgaagaactt caccacagaa tccgagggtc aacattcgtg
     1321 agagtttcgg atgccggtca tatgccccag tgggaacggc ctgatctggt caaccgatcg
     1381 ttgatcgcgt atattcaacc atcgccctga atgatccctc gactggatcg cccacttatt
     1441 atcttcagaa aggagcttac gatgcggacg agatattcag ctttcatcgg caccgcgctg
     1501 tgcttttcgc ttagcctcgt cggatgtcag acgatgaaag gatcaaccgg cgccggcttt
     1561 tcctacaaga atatttcggt ggcggatggg agcgcagtcg caggcgacgg caacaaggtc
     1621 ctgttcaaag gcaacccgct ggcgcttaca ggcaatggag tgaaggtcgg cgaccagctg
     1681 cgtgacgtaa aggtggcgca gaatgacttg tccctcgtga acatcgcgca gaccaaaggc
     1741 gcgggaaaag tgcgcgtcat cagcgtggtt ccctcgctgg atactcctgt gtgcgagcaa
     1801 cagacccatc atctgagcga gcggagtaaa ggcctcgata agatgattga actcatcact
     1861 gtgagcgtcg acacgccctt cgcccagaaa cggtttgccg aagaagccaa catcgccaac
     1921 atcaccttct tatccgacta tcggggagcc gaattcggga agacccatgg tctcttcttg
     1981 gaaggtccga acattttgac gagagcggtc atggtcgtcg acaagacgaa taccattcgc
     2041 tatctccaaa tcactccgga actctctcaa ctgccggata tggaggaagc cttacaattt
     2101 gctcgtcgat tgatcacgga aagctgagcg ggaaaaacgt tgaggctgag gtcaaggtta
     2161 aggccaaggc attcattttt ctcgtaaacc tcaaccttaa cctgaacct
//
LOCUS       DATNDD010000405         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1334206, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000405 DATNDD010000000
VERSION     DATNDD010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334206"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..513
                     /locus_tag="VN647_09865"
     CDS             160..513
                     /locus_tag="VN647_09865"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HWG97378.1"
                     /translation="MISAIHTALSGLTAIGKQIEVVAYNIANVNTEGFKKSRTEFVEI
                     PTGGVLPVVEKDDSSGPTVLRDTGGNQTMVELSNVDLGEEMVQQIVAQHSFEANLQTL
                     RAGDALLGSILDIRK"
     gene            617..2791
                     /locus_tag="VN647_09870"
     CDS             617..2791
                     /locus_tag="VN647_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWG97379.1"
                     /translation="MACPRVLLLIPPLTQLNTPYPSTAYLTGFLQSRGIEAEQADLGI
                     EMVLRLFSPDGLRTVFRQLHGQEGSLPKEALGMMRSEQAYVKMIGPVVAFLQGKTPEI
                     AARLIEPGLLPQGPRFRGRRKFERAVSVADRAKQWATFFLEDLADLVQATITPHFALS
                     RYAEHIGRSASSFDQIIAALAEPASLTDEWLLDSFWEHVNRVNPSLVGLSIPFPGNLY
                     GAFLIAKALKQHRPDLPVAIGGGYVNTELRRVADPRVFDYIDFMTFDNGERPLLSLIE
                     HISGSRPRRSLCRTMYREKDRVVYVDDPFSHDFTMNDIGCPTYRGLALDRYLTILDST
                     NPMHRLWSEGHWNKLTVAHGCYWKQCTFCDIGLTYISRYEMTPTERLIRQIEQLVAET
                     GRGGFHFVDEAAPPSALKSLALALLERRINITWWGNIRFETAFSPDLCRLLSASGCIA
                     VTAGLEAASDRLLDNMQKGITVDQTALVAAGFKDAGILIHAYLMYGCPSETIQETIDS
                     LERVRQLVAADLLQSAFWHRFTATAHSPVGLNPTSHGVRILGPEFQGFAENDLLHYDD
                     RGETPIWLGEGLRRSLLNYLERRGLDLDVRQWFDDEAPLPQVPPTWLSRLLKKRTVDD
                     HADLERRLVWLGGAVTCERIGKRATLTITGSRQDHVISLPEPEARWLEQLIGDATPRQ
                     ACQTYPHMRDVRQRFPGIESTFNAFLATSSWKKIRGAGLVLV"
     gene            complement(2895..>3087)
                     /locus_tag="VN647_09875"
     CDS             complement(2895..>3087)
                     /locus_tag="VN647_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97380.1"
                     /translation="PLYSSMRQEFEYGVNYIISGHNARISAYGRYGDIETKGVFNNYG
                     PSAQGNKADSFHVALQLQY"
BASE COUNT          659 a          896 c          826 g          706 t
ORIGIN      
        1 tgagagggtg ggacgcaaag ccgagggacc ttaacaggga cggaacatgt tccagggtca
       61 gcctagctgc cacacaaccg gtgatctgac cagtcaggct cctcccattc ccccggtgcc
      121 ttcccctgaa ttccagcctc aactgaatgg aggtgccgca tgatttccgc tatccataca
      181 gcactctcgg gcctgactgc catcggcaaa cagatcgagg ttgttgcgta caacatcgcc
      241 aacgtcaata ccgagggatt caagaaatcc agaacagagt tcgtcgaaat cccgaccggt
      301 ggggtcttgc ctgttgttga gaaagatgat tcctccggtc caactgttct tcgcgatacc
      361 ggcggcaatc agaccatggt cgagctctcg aacgtggatc tgggtgagga aatggtgcaa
      421 caaatcgtag ctcaacacag ctttgaagcg aatctccaga ccttacgagc gggagatgcc
      481 ttactcggca gcatcctgga cataagaaag tgaaggctga ggctcggatc tcggctttcg
      541 cctcaaccgt agcctcagct ttagtctcgg accttccgaa tagcattctt tctcaccttt
      601 gtgctatctc gaggccatgg cttgtccccg ggttttattg ctgattcctc ccttgactca
      661 gctgaacacc ccctacccat ccaccgccta tttaaccggg tttcttcagt cccgtggaat
      721 cgaggcagag caagccgact tgggcatcga gatggtgttg cgcctattca gtcccgatgg
      781 gctacggact gtcttccgac aacttcacgg ccaggagggc tcacttccca aggaagccct
      841 cggcatgatg agaagtgaac aggcctacgt gaaaatgatc ggaccggtcg tcgccttctt
      901 gcagggaaaa acccccgaga ttgccgctcg cttgattgaa cccggactgt tgccccaggg
      961 cccgcgattc cgaggtcgta ggaaattcga gcgcgctgta tcagtggccg acagggccaa
     1021 acaatgggcg acattcttcc tcgaagatct tgccgatttg gtgcaagcta ctattacacc
     1081 gcacttcgcc ttgagccgat atgccgagca tatcgggcgc agcgcctcat cgttcgatca
     1141 gatcatcgcc gctttggcgg agccggcgag tctgactgac gaatggctcc tggattcatt
     1201 ctgggaacac gtcaaccgag tcaacccgtc gctggtcggt ctctccatac cgtttccggg
     1261 taatctctat ggagcattcc tcattgcaaa ggcgctcaag cagcaccgtc cagatctgcc
     1321 tgtggccatc ggcggcggct atgtgaacac ggaattgcgc agggttgccg atcctcgcgt
     1381 gttcgattat atcgacttta tgacgttcga caacggtgaa cggccgctcc tctccttaat
     1441 tgaacatatt tccggctcgc gtccgcgccg atcgctgtgc cgcacgatgt atcgcgaaaa
     1501 ggatcgcgtg gtctatgtcg atgatccctt ctcacacgac ttcacgatga acgacatcgg
     1561 ctgtccaacc taccgcggcc tggcactgga tcgctacctg accattcttg acagtaccaa
     1621 tccgatgcac cgtctctggt cggagggcca ttggaataaa ctgaccgtgg cacacggctg
     1681 ctattggaaa caatgcacct tctgcgatat agggctcacc tacatcagtc ggtacgagat
     1741 gacaccgacg gagcggctca tccggcagat cgaacaactc gtagccgaaa caggccgggg
     1801 aggatttcac ttcgtggatg aggccgcacc accatcagca ttaaagtcgc tggcccttgc
     1861 cctcttggaa cggcggatca acatcacctg gtgggggaat atccgttttg agaccgcttt
     1921 ttcaccggac ctgtgccgcc ttctttccgc ctccggttgt atcgcggtga cggcgggtct
     1981 tgaggccgcc tcggaccggc tactggataa tatgcagaaa ggcattacgg tcgaccagac
     2041 cgcgttggtc gccgccgggt tcaaagatgc cggcatcctg atccacgcct atctcatgta
     2101 tggttgtcca tctgaaacga ttcaagaaac catcgactcc ctcgaacgag tccgtcagct
     2161 tgtcgcggcg gatctccttc aatcggcctt ttggcatcgt tttacagcca ccgctcacag
     2221 ccccgtgggg ctgaatccca cttcccatgg tgtgcgcatt ctcgggcctg aatttcaagg
     2281 cttcgcggaa aatgaccttc tgcattacga tgaccgaggg gaaacgccga tctggctcgg
     2341 tgaagggctg cgacgatcgc tcctaaacta ccttgaacgg cgaggactcg atctcgatgt
     2401 tcgtcagtgg tttgatgatg aggctccgct ccctcaggta cctcctacat ggctcagccg
     2461 cttgctcaaa aagcggacgg tcgatgacca tgccgactta gaacggcgtt tggtctggct
     2521 gggtggagca gtgacttgtg agcggattgg aaagagagca accctcacca ttaccggctc
     2581 caggcaggat cacgttattt cactcccgga accggaggcc cgatggttgg agcaattgat
     2641 tggagacgcc accccgcggc aagcctgtca gacctatccg catatgcggg atgtccgcca
     2701 acggtttccc ggcatcgagt cgacgttcaa cgctttcctg gctacttctt cgtggaagaa
     2761 aatccgcggt gcgggacttg tgctcgtgta attctctcaa atctttctac acgcttccaa
     2821 aaacaagagc cggaacccag catgtgggtt ccggctcttg tggagagaca gacgtcctcg
     2881 tttacgtgag ctgcttagta ttgcagctgc aacgccacgt ggaacgaatc ggccttattc
     2941 ccttgcgcac ttggcccata gttattaaag acacccttgg tttcgatgtc gccatatcga
     3001 ccgtaggcag agatacgggc attgtggccg gagatgatgt agttcacgcc atactcaaat
     3061 tcctgccgca tggagctgta cagtgga
//
LOCUS       DATNDD010000406         5376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3607429, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000406 DATNDD010000000
VERSION     DATNDD010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3607429"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..1689
                     /locus_tag="VN647_09880"
     CDS             499..1689
                     /locus_tag="VN647_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWG97381.1"
                     /translation="MPIDEITQKVSDRYAKAASTGEQMCCPTSYDMGHLKTFIPEEVL
                     NISYGCGTPAGLKTVQGGETVLDIGSGGGIDCFEASRLVGPTGHVIGIDMTDTMLEIA
                     RKNAARVAANLGYPASNVEFHKGLADAMPVDDGTIDLIISNCVINLAPDKRKVFQEMY
                     RVAKPGGRFTISDIVSDHTVPQYLVHDTRKWGDCLSGALTLTDYINGMTAAGFLGIHL
                     IKFSPWRVIDGIHFFSVTLTGYKLPPVPTTSSIRYATLRGPFSRVVDERGTTYQRGIP
                     QPIIPEEVLLLNSPPFADHFLLTADPIALDNHDPRWTAVFPAQVPCTWQGHYALLAGP
                     FIEAADDDHHIYRRGEPLEICSKTLVVLENEGYEPHFVVLNRAGDRVSGEAVTCSPNE
                     GGCC"
     gene            1689..2045
                     /locus_tag="VN647_09885"
     CDS             1689..2045
                     /locus_tag="VN647_09885"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWG97382.1"
                     /translation="MKPPLNAKMLTPRQCAMVLKALGDETRLRILESLLIGEKCVTDL
                     VQELRCSQPHASHHLRILRDSGLIEGQRDGKQVCYKVSPAIQRALGNGQGRALDFGCC
                     ELRFPETVLMTIGHAR"
     gene            2093..3130
                     /gene="arsS"
                     /locus_tag="VN647_09890"
     CDS             2093..3130
                     /gene="arsS"
                     /locus_tag="VN647_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HWG97383.1"
                     /translation="MALTLLGRHNPLASPSEQLKVLAQSGPRLSFEAQLNQAGLYPLR
                     AVEISVFQINVGKLCNQTCRHCHVDAGPDRPETMSLETAEQCVRALAKTDIPTVDITG
                     GAPELNPHFRWLVEQIRGLDRHVIDRCNLSVLLLPSQADLAEFLASHRVEIIASLPSY
                     RAGQTDAQRGDGVFEKSMEALRLLNRFGYGRPDGGLTLNLVYNPVGAFLPPKQDAIEA
                     QFKKELRTRHGIEFNRLYTITNMPISRFLEFLVESGNYEQYMTRLANAFNPAAAAGVM
                     CRYTLSVGWDGRLYDCDFNQMLDLPLDHRASSHVRDFDPAQLHDRQIVTRNHCYGCTA
                     GSGSSCGGSVT"
     gene            3268..4032
                     /locus_tag="VN647_09895"
     CDS             3268..4032
                     /locus_tag="VN647_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWG97384.1"
                     /translation="MPAADQLSGKLVITGASTLAPLIDEIGKRFESLYSKVRVDVQTG
                     GSSRGVADVRQGLADIGMVSRVMKDDEKDLHAFPVARDGVGIIVHKENPVQALTDEQV
                     VAIYMGKIINWKDVGGKDASITVVNKAEGRSTLEVFLHYFKMKNTEVKAQVVIGDNEQ
                     GIKTIAGNRHAIGYVSIGTAEYDESQGVPIKLLSVGRIAASTETIRNGTFPISRPLHI
                     ITRTPPVGLTKAFIEYAQSKAVHDIIKQQYFVPLVD"
     gene            3989..4867
                     /gene="pstC"
                     /locus_tag="VN647_09900"
     CDS             3989..4867
                     /gene="pstC"
                     /locus_tag="VN647_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWG97385.1"
                     /translation="MTLLNSNTLYRWSTDELLCWVLRGIATIAGTIVMLIVVFLIMEA
                     LPALHHVGLLRFFTDPSWHSAEGFYNLTPMLWGTLFAMTGSVLIATPLGILSAVFCHY
                     YAPLALALPYRRMIELLAGIPSVVYGFWGLVVLVPLIAEIQPPGPSLLTGILILTIMI
                     LPTITLMADASLANVPQQYIRGAAALGLPQWATIRGVVFPAAKSGLFTGVILETGRAI
                     GETMAILMVCGNVVQIPSSIFDPIRTLTANIALEMAYALGDHRAALFVSGLVLMAMIV
                     ALVLAAEWISRGRVYG"
     gene            4860..>5376
                     /locus_tag="VN647_09905"
     CDS             4860..>5376
                     /locus_tag="VN647_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97386.1"
                     /translation="MAKTMRRPQNPSEWLALILVWGTAGLVTAVFCWLLGDIVWHGLS
                     HVSWTFLTAPPENAGRRGGIGSILVSTSLILGVCLAVSLPIGIGTAVILAEFTSDHSL
                     FGRVTRRSLDVLAGVPSIVFGLFGNAFFCKTLGLGFSILSGGLTLACMVLPILIRSTE
                     EGFRAVPASYRL"
BASE COUNT         1143 a         1539 c         1429 g         1265 t
ORIGIN      
        1 ctgggctatt tgccgaattt tcccgaccat tgggcacaag gagagacctt cgaggcgctg
       61 caactcaacc tgtatcgtct aaactttgac ctgacgttgc tggaagcgct gagaaaagtt
      121 tctgaactca gtcttccata actgccacag aagacgctcg gcaccgcgag accaacggct
      181 gatgcatcga cagatgtgat cagcaaacgt catgctggca gtaagggctc ttttgcgctc
      241 ctgacctgtc ctacgtcgta ggccgcctgc caaagttgcc atttcccatc cggttacttc
      301 gaaacgcctg taagatttac tccctgaaac agacatcatt taatggacca cccaccggat
      361 tgctagccca cgtgaatgga tttgctcctt tcgtccatca tgcaatcaga atgtcccaat
      421 tgaccgcgtg cataaaggat tggtacatag agttagctct atatctatac tttgccagac
      481 caacgcttga ggccggccat gcctattgat gaaatcacac aaaaagtcag cgatcggtac
      541 gcgaaagccg ccagtaccgg cgaacaaatg tgctgcccca ccagctacga catggggcac
      601 ctcaaaacct tcattcccga agaagttcta aacatctcct atggctgcgg cacgccggcc
      661 ggcctcaaga cggtacaggg cggagagacg gtcttggaca tcggatcggg cggcggcatc
      721 gattgctttg aagcttcccg tttggttggc cccacaggcc acgtgatcgg gatcgacatg
      781 accgatacga tgctggagat cgcccgcaag aatgcggcaa gagtggctgc aaatctgggc
      841 tatcccgcat cgaacgtgga atttcacaaa ggcctggccg atgcgatgcc ggttgacgac
      901 gggacgatcg atttaatcat ctcgaattgc gtgatcaacc tcgccccgga taagcggaag
      961 gtgtttcagg aaatgtatcg ggtcgccaaa cccggaggac ggttcaccat ttccgacatt
     1021 gtatcggacc acaccgtccc acaatacctt gtccacgata cccggaaatg gggagattgt
     1081 ttgtccggag ccttgacgct taccgactac ataaacggca tgacggcagc cggatttctt
     1141 ggcatccatc tcatcaagtt ttcaccctgg cgcgtcattg acggcatcca tttcttttcc
     1201 gtcacgttga cgggttacaa gctgcctccg gtaccgacga cctcttcgat ccgctatgcc
     1261 acccttcggg ggcctttcag ccgcgtcgtc gatgagcgag gaaccacata tcagcgcggc
     1321 atcccacaac cgatcattcc ggaagaagta ctgttgttga acagtcctcc attcgccgat
     1381 cattttctcc tgactgccga ccctattgca ctggataacc atgatccacg ctggactgcc
     1441 gtgtttccgg cacaagtccc ttgcacctgg caaggccatt acgcgctgtt ggccggaccc
     1501 ttcatcgagg cggccgacga cgaccaccat atctatcgtc gtggcgaacc actggaaatc
     1561 tgttccaaga cgttggtcgt gctggagaat gaggggtacg aacctcattt tgtcgttctc
     1621 aatcgtgccg gcgatcgtgt gagcggtgaa gccgtaacct gctcccccaa cgaagggggt
     1681 tgttgctgat gaaacctcca ttaaatgcca agatgctgac cccgcgccaa tgcgccatgg
     1741 ttctcaaggc gttgggcgat gaaacgcggc tgcggatatt ggaatccttg cttatcggtg
     1801 aaaaatgcgt gacggacctt gtgcaagaac tccggtgctc tcagccccat gcctcccatc
     1861 atctccgtat cttgcgtgat tccggcttga tcgagggaca gcgagacggc aaacaggttt
     1921 gttacaaagt ctctcccgcg attcaacgag ccttgggaaa tggacaagga cgagccctgg
     1981 atttcggctg ctgtgagttg cgatttccgg agacggtgtt gatgacgatc ggtcatgcgc
     2041 gctgaagcag accgtattct gagaagcctt tcccattcta cagagcacag atatggcgct
     2101 gactctactg ggacgacaca atcccctcgc ctctccttcc gagcaactca aggtgttggc
     2161 ccagtccggc ccacgccttt cctttgaagc gcaactgaat caggccggct tatatccgct
     2221 acgtgccgtt gaaatcagcg ttttccagat taatgtcggc aaactgtgca accagacttg
     2281 ccgtcactgt catgtggatg ccggtccgga tcgtcccgag accatgtcct tagaaacggc
     2341 tgaacagtgc gttcgagctc tggcaaaaac cgatattccc acagtcgata ttacgggcgg
     2401 ggccccggaa ctcaatccgc actttcgttg gttggtcgaa cagatccgtg gtctcgaccg
     2461 gcatgtcatc gatcgctgca acctttccgt gttgctgctg ccctcgcagg ctgacctggc
     2521 ggaatttctg gccagccatc gtgtcgagat catcgcatcg cttccctcgt atcgtgccgg
     2581 ccagaccgat gcgcagcgag gcgacggcgt ctttgaaaaa tcgatggagg cactccgtct
     2641 cctgaaccgt ttcggctatg gacgaccgga cggcgggctc accctgaacc ttgtctacaa
     2701 ccccgtcggc gcattcctcc ctccgaagca agatgccata gaagcacagt tcaagaaaga
     2761 actgcgcact cgtcatggca tcgagttcaa ccggctctac acgatcacca acatgccgat
     2821 cagccgattc ttggagttcc tcgtcgaaag cggcaactat gaacaataca tgacgcgctt
     2881 ggccaacgca ttcaatcctg ccgccgccgc cggtgtcatg tgccgctata ctctgtcggt
     2941 tggatgggac ggcaggctgt atgactgcga cttcaaccag atgctcgacc tacccctcga
     3001 tcatagagcg tcgtcgcatg ttcgagactt cgatcccgcg caacttcatg atcgtcaaat
     3061 tgtgacacgt aaccactgtt acggctgcac ggcaggttcc ggttcatcct gtggtggttc
     3121 ggtcacttga ataatcacct tcgactcata tatgtagtcg tctacatgta tcgatttatt
     3181 tgttcatgtg gatggagaac gtgtgcttcg ctcgcctcgt tcttaattat cccggtcttc
     3241 gggattccgg aatcagtgac agcccagatg cccgcagcag atcaactgtc cggaaaattg
     3301 gtgattaccg gtgcaagtac tttggctcca ctgatcgacg agatcggcaa acgattcgag
     3361 agtctctact ccaaagtacg tgtcgatgtg cagaccggcg gctcatcacg cggagtcgcc
     3421 gacgtgcgtc agggactcgc cgatatcggc atggtctcgc gcgtgatgaa agacgacgaa
     3481 aaagatctgc atgcgtttcc ggtggcgcgc gacggcgttg ggatcattgt gcacaaagag
     3541 aatcctgtcc aggcgctcac cgatgagcaa gtcgtcgcga tctatatggg caagataatc
     3601 aattggaagg acgtgggcgg aaaagacgct tccatcaccg tcgtgaacaa agcagagggg
     3661 cggtctacgc tggaagtgtt cttgcactat ttcaagatga agaatacgga ggtaaaagct
     3721 caggtggtca tcggtgacaa cgaacaagga atcaagacca tagcagggaa tcgtcatgcc
     3781 atcgggtatg tctccatcgg cacggcggaa tacgatgaat cgcaaggggt accgattaag
     3841 ctgctctcag tcggaaggat tgccgcctca accgaaacca ttcggaacgg tacttttccc
     3901 atctctcgtc cattacacat catcactcgg actccgccgg ttggattgac caaagccttt
     3961 atcgagtacg cacagtctaa agccgtgcat gacattatta aacagcaata ctttgtaccg
     4021 ctggtcgact gacgagctcc tgtgctgggt cttacgcggc atcgcaacca tcgccggcac
     4081 catcgtcatg ctcatcgtgg tcttcttgat catggaagca cttcccgctc tgcaccacgt
     4141 cggtctgctg cgattcttta ccgatccttc ctggcattca gcagagggtt tttacaacct
     4201 caccccgatg ttgtggggaa cgttgttcgc catgaccggt tctgtgttga tcgccacacc
     4261 cttaggcatt ctctccgccg tcttctgtca ttactatgcg ccactggctc tcgccctccc
     4321 ctatcggcgc atgatcgagt tgctcgccgg cattccttcc gtggtctacg gattctgggg
     4381 actcgtggtg ctggtcccgc tgattgcaga gatacaaccg ccagggccca gtctcttaac
     4441 gggcatcctg atcctcacga tcatgatcct ccccaccatt acgttgatgg ccgatgccag
     4501 tcttgccaac gtacctcaac aatacatacg cggggccgcg gcattgggac tgcctcaatg
     4561 ggcgacaatc cgaggcgttg tgtttccagc ggcgaaatcg ggattgttca ccggggtgat
     4621 cctggaaacc ggccgagcca tcggggaaac gatggcgatt ctgatggtct gtgggaacgt
     4681 ggtccagatc ccttccagca tcttcgaccc gatccggacg ctgaccgcca atatcgccct
     4741 ggaaatggcc tatgcgctcg gggaccatcg cgctgcattg ttcgtcagtg gactcgtctt
     4801 gatggccatg atcgtggcgc ttgttctagc cgctgaatgg attagtcgcg ggagagtgta
     4861 tggctaagac gatgaggcga ccacagaatc ccagtgaatg gcttgcattg attctcgtct
     4921 ggggaacggc agggctcgtg accgccgtat tctgctggct gttgggggac atcgtttggc
     4981 atggactgag ccacgtctcc tggacatttc tgacagcccc gccggaaaat gccggtcgcc
     5041 gaggcgggat cggctctatc ctggtttcga catccttgat tctgggagtc tgtctcgcgg
     5101 tatcgctccc tattggtatc ggcaccgccg tcatcctcgc tgaatttacg tcggatcaca
     5161 gcctgtttgg acgggtgact cgccgcagcc ttgatgttct agccggtgtg ccgtcgatcg
     5221 tcttcggtct gttcggcaac gcgttctttt gcaagacgct ggggctcggc ttttcaatcc
     5281 tttccggcgg gctgactctg gcctgcatgg tgttgccgat cttgatccga tcaacagagg
     5341 aaggctttcg cgccgtgccg gctagttacc ggcttt
//
LOCUS       DATNDD010000407         3443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_770145, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000407 DATNDD010000000
VERSION     DATNDD010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770145"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /gene="rpsC"
                     /locus_tag="VN647_09910"
     CDS             <1..289
                     /gene="rpsC"
                     /locus_tag="VN647_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWG97387.1"
                     /translation="FRRAMKRSVQSALRLGAQGIKIMVAGRLGGAEIARTEWYREGRV
                     PLHTLRAEIDYGFAEAHTTMGQIGVKTWIYKGELLPLQPIKAESSLDRRFG"
     gene            302..718
                     /gene="rplP"
                     /locus_tag="VN647_09915"
     CDS             302..718
                     /gene="rplP"
                     /locus_tag="VN647_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWG97388.1"
                     /translation="MLAPKKVKFRKMQKGRMTGKAYRGGQITLGEFGLKALEPGWVTS
                     RQIEAARIAITRYVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     LYEMDGVTSETAREAFRLASHKLPIATKLVVRGELG"
     gene            750..965
                     /gene="rpmC"
                     /locus_tag="VN647_09920"
     CDS             750..965
                     /gene="rpmC"
                     /locus_tag="VN647_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWG97389.1"
                     /translation="MALDIKELQQLGASELIDKEKQLVQELFNLRFQFGSGRLENPTQ
                     IRMTKRDIARVKTVLQQVKARAEGAKR"
     gene            983..1240
                     /gene="rpsQ"
                     /locus_tag="VN647_09925"
     CDS             983..1240
                     /gene="rpsQ"
                     /locus_tag="VN647_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWG97390.1"
                     /translation="MVKRRHWYGDVVSNKMQKTVVVVVSRSVVHPIYKKVLRRVTRLK
                     AHDESGVCKVGDRVKLVQSRPLSKEKNWRVVQVMEKGQPEK"
     gene            1293..1661
                     /gene="rplN"
                     /locus_tag="VN647_09930"
     CDS             1293..1661
                     /gene="rplN"
                     /locus_tag="VN647_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWG97391.1"
                     /translation="MIQNYTYMDVADNSGAKQAMCFHVFGGTRRRYASLGDVVVVAVK
                     EAIPQASVKKGDVSRAVIVRTTKEVRREDGSYIKFDRNACVLINKEGEPIGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            1684..2013
                     /gene="rplX"
                     /locus_tag="VN647_09935"
     CDS             1684..2013
                     /gene="rplX"
                     /locus_tag="VN647_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWG97392.1"
                     /translation="MEALRKSRIRKGDTVVVVSGRERGKTGKVLSIDLRAGKVVVEKL
                     NIIKRHTKPNQKAKQGGILEKEAPLQISNVMFLCPVTQKPTRIGIRVLEDGRRVRFSK
                     KSNESVE"
     gene            2022..2666
                     /gene="rplE"
                     /locus_tag="VN647_09940"
     CDS             2022..2666
                     /gene="rplE"
                     /locus_tag="VN647_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWG97393.1"
                     /translation="MAKEPKNRPSKPSDRKSSKKEPAPQLDQGSEESKFRPRLRDMYE
                     QQAVPALMKEFGYKNVMQVPKLERVVLNVGMGEAIQNVKLLESAVTELGMITGQKPVV
                     TRAKKAIAGFKLRQGLPIGAKVTLRSRRMYEFFDRLVTLALPRIRDFRGVSPKAFDGR
                     GNYTLGLKEQLIFPEIKYDEVASIHGMDITVVTTARTNDEGKALLKHLGMPFRA"
     gene            2712..2897
                     /locus_tag="VN647_09945"
     CDS             2712..2897
                     /locus_tag="VN647_09945"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWG97394.1"
                     /translation="MSRLALRNKAATKQKFSTRDYHRCGVCGRVRGYLRRFRMCRICF
                     RVMTLRGEIPGVRKSSW"
     gene            2947..3342
                     /gene="rpsH"
                     /locus_tag="VN647_09950"
     CDS             2947..3342
                     /gene="rpsH"
                     /locus_tag="VN647_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWG97395.1"
                     /translation="MVTDPIGDLLVRLKNGAQRRYETVTVPTSKLKRAILEILRKEGY
                     VDAIEDGVHDGHPILTVRLRYVGEGQPMITGLERVSKPGRRVYVGSQDIKKVRNGIGV
                     SILSTSKGIMTDQESRKSRLGGEVLCSVW"
BASE COUNT          879 a          746 c         1076 g          742 t
ORIGIN      
        1 ttttcggaga gccatgaaac gaagcgttca gtctgccctg aggcttggtg ctcaaggtat
       61 caagatcatg gtggccggtc gcctgggtgg tgccgaaatc gccaggacgg agtggtatcg
      121 agaagggcgt gtgccgctgc acacgctccg tgccgaaatt gattatggat tcgccgaagc
      181 gcacacgacg atggggcaga tcggggtgaa gacctggatc tataaagggg agcttcttcc
      241 tcttcagcct atcaaggcag aatcatcttt agatcgacgg tttgggtgag gagatcgagc
      301 tgtgttagcg cctaagaaag tcaaattcag gaagatgcag aaaggccgga tgacgggtaa
      361 ggcctatcgc ggtgggcaga ttaccttggg ggagtttggt ctgaaggcat tggaaccagg
      421 gtgggtcacc agccgacaaa ttgaggccgc gcgtattgct attactcggt atgtgaaacg
      481 gggagggcaa gtatggacac gcattttccc cgacaagccg atcactaaaa agccggccga
      541 aactcgaatg ggtaagggaa agggcaaccc tgagtactgg gttgctgtcg tgaagccagg
      601 caggattctt tacgagatgg atggagtcac ttccgaaacc gctcgagagg cgtttcgtct
      661 tgcttcgcat aagttgccga ttgccacgaa gttggtcgtc cgcggcgagc ttgggtaagt
      721 tcttgaggat ataacaaggg acaagggata tggcgttgga catcaaagag ctccagcagc
      781 tgggggcgag tgaacttata gacaaagaaa aacagctggt gcaagagctg ttcaatctac
      841 gttttcagtt cggctccggg cgtctcgaga atcccacgca aatccggatg acgaagcggg
      901 atattgcacg ggtgaaaaca gttctccagc aagtgaaggc ccgagcagag ggggctaaaa
      961 ggtaggggac gctatggctg agatggtaaa acgtcgccat tggtatggtg acgtcgtcag
     1021 taacaagatg caaaagacgg tcgtcgtcgt ggtctcgagg tcggtcgttc acccgatcta
     1081 taaaaaggtt ctgaggcgag taaccaggct gaaggcccac gatgaaagtg gtgtgtgtaa
     1141 ggtgggagat cgggtcaagc tcgtgcagtc caggcctctg agcaaagaaa agaactggcg
     1201 cgtcgttcaa gtcatggaaa aaggtcaacc cgaaaagtag gaagcgatgt ttcttcgtat
     1261 cctccgtttt tgttgcgggt aggaatatag cgatgattca gaactatacg tatatggatg
     1321 tggccgataa ttcgggagcg aaacaggcca tgtgttttca tgttttcggg gggacgcgac
     1381 gacgctatgc gtcgctcgga gatgtcgtgg tggtggcagt caaggaggcg atccctcaag
     1441 ccagcgtgaa gaagggggat gtgagtcggg ccgtcattgt ccgaacgacg aaggaggttc
     1501 gtcgggagga tggatcttat attaagtttg atcgaaatgc gtgcgtcttg atcaacaaag
     1561 agggcgagcc gattggaacg cgtatattcg gtcccgtcgc ccgcgagctc cgctggaaga
     1621 aattcatgaa aatcatttcc ttggcacctg aagttttgta gggcagaacg aacgagaggc
     1681 atcgtggaag cactccgaaa aagtagaatc cgaaaagggg atacggtcgt tgtggtttct
     1741 ggtcgtgaac gaggcaagac cgggaaggtt ctgtcgatcg acctgcgtgc cgggaaggtg
     1801 gttgtggaaa agctgaatat catcaagcga cacaccaagc cgaaccagaa ggcgaagcag
     1861 gggggcattc tggagaaaga ggcacctctt cagatttcca acgtcatgtt cctctgcccg
     1921 gtcacacaga agcccacccg gatagggatt cgggttttgg aagacgggcg acgcgtgcgc
     1981 ttcagtaaga aatccaacga aagcgtggaa taggttacgt aatggccaag gaaccgaaaa
     2041 acagacctag taagccttca gatcgaaaat cctcgaagaa ggagccggcc ccgcaattgg
     2101 accagggaag cgaggagtcc aagtttcgac cacggcttcg cgacatgtac gaacaacagg
     2161 cggtccccgc actcatgaag gagtttgggt acaagaacgt gatgcaggtt cccaagcttg
     2221 agcgtgtggt gttgaacgtc ggaatgggtg aggcgattca gaatgtgaag ctcttggaga
     2281 gtgcggtgac tgaattggga atgatcaccg ggcaaaagcc ggtcgtcact cgagccaaaa
     2341 aagccatcgc gggattcaag ctcagacaag gattgcccat cggtgcgaag gtgacgctcc
     2401 gtagccgacg gatgtatgag tttttcgatc gactagtgac gttggcgctt cctcgaatac
     2461 gagacttccg cggcgtctct cccaaggcct ttgatggtcg ggggaactat acccttggct
     2521 taaaagagca gctcatcttt cctgaaatca aatacgacga agtcgcctcg atccatggga
     2581 tggacattac agtcgtcacc acagccagga cgaacgacga agggaaagct cttttgaagc
     2641 atttaggtat gccgttccgc gcgtgagagc gacgtgaaac aacggcgtct gtttgaacgg
     2701 aaggagtgct tgtgtcacga ttggcactga gaaataaggc ggcaacgaaa caaaagttct
     2761 ccacgcggga ctatcatcgg tgtggagtct gtggtcgagt tcgcggatat ctccgtcggt
     2821 ttcggatgtg tcgaatttgt tttcgagtaa tgactctgcg cggagagatt cccggagtgc
     2881 gaaaatctag ctggtagctg gtgttctcac gccggtgacc gccaatggag gatagaagga
     2941 tatagcatgg ttaccgatcc aatcggcgat cttcttgttc gtttaaagaa tggcgctcaa
     3001 cgccgttatg aaacggtcac ggttccaacc tcaaaactga aacgtgccat tttggagatt
     3061 ttgaggaagg agggttatgt ggacgcaatc gaggatgggg ttcatgacgg acatcccatt
     3121 cttaccgtgc gtcttcgata tgtgggcgag gggcagccga tgatcaccgg actggagcgc
     3181 gtcagcaaac cgggacgccg agtctatgtc ggcagccagg atatcaagaa agtccgcaat
     3241 gggatcggtg tgtccatcct ttcaacctca aaaggaatca tgacggacca agaatcccgt
     3301 aagagtcgtc ttgggggtga ggttctctgc tcggtatggt aggcggatga gtcatcttga
     3361 tcttgagtga agggtataac agaccatgtc ccgcattgga aaaaagccga ttgcaattcc
     3421 gggtggagtg gaagtcaaag ttg
//
LOCUS       DATNDD010000408         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2759294, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000408 DATNDD010000000
VERSION     DATNDD010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759294"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..517)
                     /locus_tag="VN647_09955"
     CDS             complement(176..517)
                     /locus_tag="VN647_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97396.1"
                     /translation="MKLFLMFLFFSGAAYLTVVFNWSYSDGSRAGYIQKFSSKGWLCK
                     THEGELAMTTVPGTAPVLWDFTIWDDKVATQLSEMMGKRVILHYKEYRYIPTTCFGET
                     TYFVDKVEVQE"
     gene            complement(532..1155)
                     /gene="leuD"
                     /locus_tag="VN647_09960"
     CDS             complement(532..1155)
                     /gene="leuD"
                     /locus_tag="VN647_09960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWG97397.1"
                     /translation="MQPLTTLTGLVAPLDRVNVDTDQVIPKQFLKTIKRTGLREGLFF
                     DWRKLKDGSSDPSFFLNQPRYQDATILLTRDNFGCGSSREHAPWALLDQGFRCIIAPS
                     FADIFYNNCFQNGILPVVLRAEEVLPLMKDVIDTEGYRLTVDLHHQTVTAPKGTTYRF
                     EIDPFRKDCLYQGLDAIGLTLQHEKTITAYESRRKTEAPWLFDDVHP"
     gene            complement(1210..>2025)
                     /locus_tag="VN647_09965"
     CDS             complement(1210..>2025)
                     /locus_tag="VN647_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWG97398.1"
                     /translation="GTGYVIEYTGSAINALSMEGRMTLCNMSIEGGARAGMVAPDDKT
                     VAYIKDRPLAPKGELFAQATQAWRQLKTDSDAKYDATVTLQAEHIAPQVSWGTSPGMV
                     IGVDQKVPDPRTMSDEKAKNATERALAYMGLSPNMPITDIKIDTVFIGSCTNSRIEDL
                     RLAAGFAKGRKVAKTVHAMVVPGSGLVKQQAESEGLDRVFREAGFEWREAGCSMCLAM
                     NADVLKPGERCASTNNRNFEGRQGAGGRTHLVSPAMAVAAAVEGHFVDIRHWG"
BASE COUNT          452 a          572 c          526 g          475 t
ORIGIN      
        1 tatgctgatt gggttccgtc gaaagaaatg gctgtagcag gatgttgaaa aagccctcca
       61 gtttcgttct cgcatcgctc aaggcctcaa cgtaccaacc gcgtacgcct cagcctttcg
      121 cttgctccgg ccttgctgga aggacttttt gaacatcctg ggagttgata cacacctatt
      181 cttgaacttc caccttatct acgaagtagg tggtctcacc gaaacaggtg gttggaatat
      241 agcgatactc tttgtaatgc aagatcaccc gtttccccat catctctgac aattgcgtgg
      301 cgactttatc gtcccagatc gtaaaatccc agagaacagg cgccgttcca ggcacggtcg
      361 tcattgccaa ctctccttcg tgcgtcttac ataaccaacc cttcgaggaa aatttttgga
      421 tgtatccggc tcggcttcca tcggagtagg accagttgaa cacgacagtg aggtaggcag
      481 cacctgaaaa gaagagaaac atcaaaaaca gtttcactaa tatttcctca gttatggatg
      541 gacgtcgtcg aacagccaag gagcctcggt ttttcgccga gattcatagg ccgtgatcgt
      601 cttctcgtgc tggagcgtga ggccgatcgc atcaagcccc tgatagaggc aatccttgcg
      661 gaaagggtcg atttcgaacc gataggtggt tcctttcgga gcggtcactg tttgatggtg
      721 caggtccacg gtgagacggt aaccctcggt gtcgatcacg tccttcatga gcggcagcac
      781 ctcttcggct ctcaacacca ctgggaggat cccattctga aaacaattgt tatagaaaat
      841 gtccgcaaag ctcggggcga tgatgcaccg gaacccttga tccaataatg cccaaggcgc
      901 atgctcacgg gatgagccac agccgaagtt gtctcgggtc aggagaatcg tcgcatcctg
      961 atatcgaggt tggttcaaaa agaaggacgg atcagatgaa ccgtctttga gttttcgcca
     1021 atcgaagaac agaccttcgc gcaacccagt ccgtttgatg gtcttaagaa attgcttcgg
     1081 aatcacttgg tcggtgtcga cgttcacgcg atccagggga gcaacgagac cggtcagggt
     1141 agtcaagggt tgcatagtgg tttgcttcat gacgataggt ggatattgaa ccagagatgc
     1201 attgactact tatccccagt gacggatatc gacaaagtgt ccttcaacgg ccgcggcgac
     1261 cgccatggcc ggcgacacca gatgggtgcg acctccggct ccctgacgcc cctcaaaatt
     1321 ccgattgttc gtggaggcgc aacgctcgcc gggcttcagc acatcggcat tcatcgcgag
     1381 acacatgctg cacccggcct cccgccattc gaaccctgcc tcccggaaca cccggtccag
     1441 tccttcgctc tcggcctgtt gcttgaccag ccccgatccc ggaaccacca tcgcatgcac
     1501 ggtcttcgcg actttcctgc ccttcgcgaa accggcggcg agccgaagat cttcgatccg
     1561 cgagttcgta caggagccga tgaacaccgt atcgatctta atatcagtaa tcggcatatt
     1621 gggtgaaagc cccatgtagg ctaatgcccg ttcggtggca ttcttcgctt tctcgtccga
     1681 catcgttcgg ggatccggga ctttttgatc cacgccgatg accataccgg gactggtgcc
     1741 ccagctgact tgcggcgcga tatgttctgc ctgcaatgtt acggtggcat catacttggc
     1801 atcagaatcg gtcttgagct gccgccatgc ctgagtcgcc tgtgcgaaca gctctccctt
     1861 tggcgccaac ggccggtcct tgatataggc aacagtcttg tcgtcaggag caaccatgcc
     1921 tgcacgagcc ccaccctcga tcgacatgtt acagagggtc atacgacctt ccatgctgag
     1981 ggcgttgatt gccgaaccgg tatattcgat gacatagccg gtccc
//
LOCUS       DATNDD010000409         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3126152, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000409 DATNDD010000000
VERSION     DATNDD010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3126152"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..401)
                     /locus_tag="VN647_09970"
     CDS             complement(108..401)
                     /locus_tag="VN647_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97399.1"
                     /translation="MDRSMVATAWEQHCAEGWPHFSSPDQGQLMTIDTVISGCVVYFL
                     DSSEGLDDPRVAIVKDCLGDLDELTKALDTEPQAYFYRLRELGAMLLGDESQA"
     gene            complement(497..1747)
                     /locus_tag="VN647_09975"
     CDS             complement(497..1747)
                     /locus_tag="VN647_09975"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWG97400.1"
                     /translation="MRELDTPTFRLAVAQFDQAAEAMGLDQNLRERLKLPQRSLVVSL
                     PVRMDDGHVEVFTGYRIQHDSSRGPSKGGVRYHPDVNLGEVAALAMWMTWKCALAGLP
                     YGGAKGGVAVAPKQLSSAELQRLTRRYAAEIFPLIGPDKDVPAPDVGTDAQIMAWMMD
                     TYSQQVGYAVPGVVTGKPLSIGGSLGREEATGRGVVYVTQEVLRHLKLSIENATVAIQ
                     GFGNVGSHTARIMQQQGARVIAVSDVHGGIYNPKGLDVMELLRRDPSQPLHNAKLGDA
                     VTNEELLQLECTVLVPAALSEQITSKNANYLRCRILSEGANGPTTLEADRILAEKDIF
                     VIPDILANSGGVIVSYFEWVQDLQRFFWSATDIQNRLQGIITSAFQRTLHFSTERRVS
                     MRMAALMSGIDQVAQAHLQRGLYP"
     gene            complement(1793..2506)
                     /gene="lipB"
                     /locus_tag="VN647_09980"
     CDS             complement(1793..2506)
                     /gene="lipB"
                     /locus_tag="VN647_09980"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWG97401.1"
                     /translation="MIDANTLTSPHLSSSSIHAGAVRIFSDPVPYLIAWEIQSRLHEE
                     RLLDLQPDTVLILEHPPVYTLGRRTNLSHWGGNESALCADGAELHHVNRGGSVTFHGP
                     GQIVVYPVLKLERHAAGPRRLIWLLEEVIIRVLDHWNITGSRVVGKPGVWIMTPEPQK
                     IAFLGIRIEHGVTLHGFALNVDLALTPFQRIHPCGFPDCRVTSMAAVRQTAVPVGVVK
                     QELAQTFAAVFDLNWSTVA"
     gene            complement(2704..>2838)
                     /locus_tag="VN647_09985"
     CDS             complement(2704..>2838)
                     /locus_tag="VN647_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWG97402.1"
                     /translation="DEASTLEQVGQSLSVTRERIRQIEAKALKKLKTPEIKELFAAIK
                     "
BASE COUNT          659 a          775 c          770 g          634 t
ORIGIN      
        1 actggtatat gacacattgt agccaagagc tattagacgt gcaaggaacg ggatgcgaga
       61 acggctgctg gacaaacgac tcgacgagtc ggcttagtgt tctcgtatca tgcttgagac
      121 tcatcaccca gcaacatcgc tccgagttca cgaagccggt agaagtaggc ttggggctct
      181 gtgtccaatg ctttcgtcag ttcatcgaga tctcccaaac agtctttcac gatggcaacc
      241 cgtgggtcgt cgagaccctc ggaactatcg agaaaataga ccacacaacc gctaatgacc
      301 gtgtcgatcg tcatcaattg tccttgatct gggctcgaaa aatgaggcca cccctcggca
      361 caatgctgtt cccacgctgt tgcaaccatg cttcgatcca tgaggtgctc ctttccctct
      421 ccgccatgtg acaagccagc tccttagtga aggtgggccg taacgagcaa gtatcaaacc
      481 aggaggaatc gcaggatcag ggatacagtc cacgttggag atgtgcttga gcaacttgat
      541 caatcccact catgagggct gccatacgca tcgatactcg gcgttccgtc gaaaaatgaa
      601 gtgtccgctg aaaggcggac gtgatgatgc cttgcagccg attctggatg tcagttgcac
      661 tccagaaaaa tcgctgaagg tcctgcaccc attcgaagta tgagacaatg acgccgccgg
      721 agttggcgag aatatcgggg attacgaaga tgtctttctc tgcaaggatg cggtcggcct
      781 ccagcgtcgt cggaccgttc gctccttccg acaaaatccg acacctcaaa taattggcat
      841 ttttacttgt aatctgttcc gagagtgcgg cgggtacgag aacagtacat tctagttgca
      901 gaagttcctc gtttgtgacc gcatctccca gtttggcatt atgcagcggt tggctaggat
      961 cgcgacgaag caactccatg acatccaacc ccttggggtt gtaaattcca ccatgtacat
     1021 cgctgacggc gatgacgcgt gcaccttgtt gctgcatgat gcgggcggtg tgtgaaccga
     1081 cattgccgaa tccttgaatt gccacggtgg cattttcaat ggacaactta aggtgacgaa
     1141 gcacctcttg cgtcacatag acgaccccgc gtcccgtcgc ttcttcacgg cccaagctcc
     1201 ctccgatcga gagcggtttg ccggtcacca ctccaggaac ggcgtagccg acttgttgac
     1261 tgtatgtgtc catcatccat gccatgattt gagcatcggt tcccacgtct ggggccggta
     1321 catccttgtc cgggccaatc agcgggaaaa tctctgcggc gtatcgtcgt gtcaaccgct
     1381 gtaactcggc agaggacagt tgtttcggag ctactgcgac tccgcctttt gcacctccgt
     1441 acggcaaccc ggccaacgcg catttccacg tcatccacat cgcaagagcg gccacctcac
     1501 ccagattcac gtcgggatgg taacgcacgc cgcccttgga cgggccccgt gacgaatcat
     1561 gttgaatgcg gtagccggta aaaacctcaa cgtgcccgtc gtccatccga accggaaggc
     1621 tgacgacgag agaccgctgt ggaagcttca gtcgttcacg gaggttttgg tcaagtccca
     1681 tcgcttccgc cgcctgatcg aactgtgcga cggccaggcg aaaagtcggg gtatcaagtt
     1741 cccgcatagg actcctccac gcaagcacca gcctgtttcg tacgtcggct ggtcatgcaa
     1801 cagtcgacca gttgaggtca aataccgccg cgaaggtctg agcaagttcc tgcttgacga
     1861 cgccaaccgg gacggctgtt tggcgtactg ctgccatgga tgtcactcgg caatccggaa
     1921 atccacaggg atggatccgt tggaacggcg tcagagccaa atcaacattc agagcaaatc
     1981 cgtgcaatgt cacgccgtgc tcaattcgta tgcccaggaa tgcgattttc tgtggctccg
     2041 gagtcatgat ccatacacct ggtttaccta cgacacggga gccggtaatg ttccagtgat
     2101 cgagtacccg aatgatcact tcttccagta accagatcaa tcgcctgggt cccgctgcat
     2161 gtcgttcaag cttgagcacg gggtagacca caatctgtcc tgggccgtga aaggtcacag
     2221 agcctcctcg attgacgtga tgcagttccg cgccatccgc acataacgcg gattcattgc
     2281 ctccccaatg cgacagattg gtcctgcgtc cgagcgtata gacgggtgga tgttcaagga
     2341 tcagaacagt atccggttgg agatcgagga gacgctcctc atgcaagcga gactgtattt
     2401 cccacgcaat gagatagggg accggatctg aaaagatgcg aaccgcgccc gcatgaatag
     2461 aggaagagct caaatgcgga gacgtcaggg tattggcatc gatcatgaga ttccacgctc
     2521 aaagtgatag atccatccat gaagatggtg aaacacccgc cggaggtcgg ttccgaagga
     2581 tgcggtagtg gaaagaaaga acgggctccg catagcggga atattgttgc ataacttacc
     2641 atacagagga aagtctggtt ggaggatcac ggggctgctg gcgcagcccc gtgaatcaaa
     2701 gagttactta atggcggcga agagttcctt gatctcaggg gtcttcagtt tcttgagtgc
     2761 ttttgcttca atttggcgga tacgctcgcg tgtcacggac aagctttggc cgacttgctc
     2821 caaggtgctg gcttcatc
//
LOCUS       DATNDD010000410         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2762841, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000410 DATNDD010000000
VERSION     DATNDD010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762841"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VN647_09990"
     CDS             <1..1133
                     /locus_tag="VN647_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HWG97403.1"
                     /translation="ADLKTMPHLLVAGATGAGKSVSLNTMLLSILFSAKPSEVKLLLI
                     DPKMLEFQSYEGIPHLLRPVITEPKSAARGLGWVVAEMERRYKLLAEAGVRNIDAYNR
                     KVAGLHEVFAENNPAGIEQTELPMQFLSEEERLSAGETSIAEGERGCMQPKPTPPEPL
                     PFIVVMIDELADLMMVAPKDVEDKIARLAQMARASGIHLVLATQRPSVDVLTGLIKAN
                     FPARIAFQVSSKTDSRTILDANGAEALLGRGDMLYLASGTGRLARLHGSFVSDDDVRS
                     VVEFVKKQALPIYNQELQSLKLEEAAEEEAKDEVYEQAKELVLSTGQASASLIQRRLR
                     VGYPRAARMIEQMESEGIVGAAGRDGRREVLGRRGPVGAAEA"
     gene            1130..1840
                     /locus_tag="VN647_09995"
     CDS             1130..1840
                     /locus_tag="VN647_09995"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWG97404.1"
                     /translation="MMHWWLAAPLSVVVFVLPVWAADGPIDEKAWREVREVVKQLQAR
                     YEKTKDLQADFSQKTKIEGFERPVTSSGKVYIKKPGRLRWDYLDPAIEQIYVNQDDVK
                     VYVPEHKQVLVGKLTQMAASKAPLELLQGASKLDESFEIEPTMGKDRGVGGTPLITLI
                     PKAKEQESTQNLQKIIVEVFPKTHYIRTVSLHEISGNVAVFEFSNLKPNLGLGNEVFD
                     FKTPSDAEVVRAPVLNGP"
     gene            1885..>2177
                     /locus_tag="VN647_10000"
     CDS             1885..>2177
                     /locus_tag="VN647_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWG97405.1"
                     /translation="MKNVTDTVDQAVAALDVDRLHREYWEQNEFLVIKQFLPRAFVED
                     VFVPQAQGVKAELNRNFIPGHKKGGSVSYYTVQEKAPRFLDLYQSESFRTFLNR"
BASE COUNT          501 a          556 c          663 g          457 t
ORIGIN      
        1 ccgccgatct gaagacgatg ccgcatctct tggtagccgg cgcgactggt gccggaaaga
       61 gtgtcagttt gaatacgatg ttgctcagca tcctcttttc cgccaagccc agtgaagtga
      121 agctcctgct cattgacccg aagatgcttg agtttcaatc gtacgagggc attcctcatc
      181 tgttgcgacc ggtgattacg gagcccaaat cagccgcgag agggctgggg tgggtcgtcg
      241 ctgaaatgga acggcggtac aagctgctgg ccgaggccgg tgtacggaat atcgatgcat
      301 acaatcgaaa ggttgcaggc ttgcatgagg tgtttgccga aaacaacccg gcgggcatcg
      361 agcagaccga gcttccaatg cagtttctct ctgaagaaga gcgcctctct gccggtgaaa
      421 catcgatcgc cgagggtgag cgcggttgca tgcagccgaa accgacgccg ccggaaccgc
      481 tgccgtttat cgtcgtcatg atcgacgagc tcgcggatct gatgatggtg gctcccaaag
      541 atgtggaaga caagatcgcc cgacttgcac agatggcgcg ggcttccggc attcatctag
      601 tgttggccac tcagcgcccg tccgtcgatg tgctgaccgg cttgatcaaa gcgaattttc
      661 cggcacggat cgcgtttcaa gtatcttcga agaccgactc acgaaccatt ctcgatgcga
      721 acggagcgga ggctctactc ggccgaggcg atatgctgta tctggcctca ggcaccggcc
      781 gcctcgcacg gctgcacggc tcctttgtat cggacgacga tgttcgctca gtcgtggaat
      841 tcgtgaagaa acaagccctt ccgatttaca atcaggaact gcagtcgctt aagttggagg
      901 aggcggcgga agaagaggca aaagatgaag tgtacgaaca ggctaaagaa ttggtcctgt
      961 cgaccgggca ggcttcagcc tcgttgattc agcgccgact gcgagtcggc tatcctcggg
     1021 cggcacgcat gatcgaacag atggaatcgg agggcattgt gggggcggcg ggtcgtgatg
     1081 ggcgtcggga ggtgcttggt cggcgcggtc cggtcggcgc ggcggaagca tgatgcattg
     1141 gtggcttgcc gctccattga gtgttgttgt gttcgtcttg ccggtctggg cggcagacgg
     1201 gccgattgac gaaaaggcat ggcgggaagt tcgtgaagtc gtcaaacagc tgcaagcacg
     1261 atacgaaaaa acgaaggatc tgcaggccga tttttctcag aaaaccaaga ttgaggggtt
     1321 tgagcggccg gtgacgtcgt ccggcaaggt gtatatcaaa aagcccggcc gattacggtg
     1381 ggactatttg gatccggcga tcgagcaaat ctatgtgaac caggacgatg tgaaggtgta
     1441 cgttcccgaa cataagcagg tcctagtcgg gaaactgacg cagatggctg cctccaaagc
     1501 gccgttggaa ttattgcagg gagcgtccaa attggatgag tcgtttgaga ttgagcccac
     1561 catgggaaaa gaccggggag tcggtggtac gccgcttatc acactgattc ccaaagccaa
     1621 ggagcaggaa tccacccaga atcttcagaa gatcatcgtc gaggtcttcc cgaaaaccca
     1681 ttatatccgc acggtctcac tccatgagat cagcggcaac gtcgccgtct ttgaattttc
     1741 aaacttgaaa ccaaacctgg gattgggtaa tgaggtgttc gactttaaga caccgtccga
     1801 tgcggaagtc gtcagggctc cggtgttgaa cgggccgtag gacttatgcg cgaacgggag
     1861 acagctcgac cgaggacaaa tctcatgaag aacgtgacgg acacggtcga tcaagccgtc
     1921 gcagccttgg acgttgatcg tcttcaccgg gaatattggg agcaaaacga gttcctggtg
     1981 atcaaacaat tcttgccccg tgccttcgtg gaggacgtgt ttgttcccca ggcccagggc
     2041 gtgaaagcgg agctcaatcg aaactttatt cccggccaca agaaaggcgg gagcgtcagc
     2101 tattataccg tgcaagaaaa agctccccgc tttctcgacc tctaccagtc tgagtcgttc
     2161 cggacattcc tgaaccg
//
LOCUS       DATNDD010000411         3380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3624387, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000411 DATNDD010000000
VERSION     DATNDD010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3624387"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VN647_10005"
     CDS             <1..694
                     /locus_tag="VN647_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWG97406.1"
                     /translation="YHTYGLRDRDGAAPDFKATDISLRQWGAEFRTYFRGRNLGPFRL
                     AVPGIHNVSNALVAIAIGFELEIPVDLIRKGLAAFTGVERRFHLRGEAGGIMVVDDYG
                     HHPTEVKATLTAAKQGWDRRLVVLFQPHRYSRTRDCIGEFAHAFDYADLLFTTEIYPA
                     GESPIPGVSGAALAETIKSTGHPSVSFIERKETLPDQVLPHLRPGDLVLTLGAGDIWK
                     AGTGILARLEST"
     gene            694..1644
                     /gene="murB"
                     /locus_tag="VN647_10010"
     CDS             694..1644
                     /gene="murB"
                     /locus_tag="VN647_10010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWG97407.1"
                     /translation="MAQKHRVQTDGTKVRPVRVQHRLESAVAGVRGLVRFNAPLKEYT
                     SFHIGGPADVLVEPADVEDIVRLAKQTREQKIPIFVLGGTNILIRDKGIRGVVVDLAK
                     LRAIKEEPGSVLYAEGGVGMPTLIGQAIRRSLAGLEWAAGIPGTVAGCVVMNAGTRLG
                     EMKDSVKAVRIVSPKGALIDCPAEAIEFRYRRATLPQGIVVGVWLQLRVGVRSDIEKV
                     VKNYLHYRRDTQPLTLPSAGCVFKNPLNDSAGRVVEAAGLKGTSVGDAQVSIKHANFI
                     VNQGHASAADVLSLIKKVRTRIAQEMGIKLELELKIVGEV"
     gene            1660..2643
                     /locus_tag="VN647_10015"
     CDS             1660..2643
                     /locus_tag="VN647_10015"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWG97408.1"
                     /translation="MAIDLMTHARIGVLMGGRSSERDISLKTGQAVYQALIRRGYDAV
                     AIDVTDRLHRDLEDHKVAIVFLSLHGPGGEDGTVQGFLETLGIPYTGSGVRASAVGMH
                     KVATKTILAAHGIPLPAGTVVRECDRPSLAKVLRQAKLKLPVVVKPVSQGSTIGVTVV
                     RRAGQWREALDLAHRYDPEVMVESYIPGHEATVSILGTAAEGPKVLPAIEIVAPDGFY
                     DFSAKYQKGRTRYLCPAPLPPKVVHHIRELAGRTYEVLGCEGAVRVDFRITPRGRPYV
                     LEVNTVPGMTETSLLPMAAAQAGIAYDDLVEQILQSALDRASRFARVVPKE"
     gene            2733..>3380
                     /locus_tag="VN647_10020"
     CDS             2733..>3380
                     /locus_tag="VN647_10020"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWG97409.1"
                     /translation="MKEAGRAKIARRKALVRRVGLATGAGLVAWFIVMGMRYSGPALQ
                     TLLEIRTITIEGEHHIDKQKVLELAKVKPGTGLHHIVTRVIKEQVESHPWVKEAEVTR
                     VAFHELRISLVERIPAAVIRADAQNFLTDAEGHVLTKLGQIDDEALPFMTGIDSQGLL
                     DGTESVRQVIASGIKLAKLVGQTFEGRLQVNAENPSNLVALVQGMRFQYGEEAVGE"
BASE COUNT          758 a          829 c         1070 g          723 t
ORIGIN      
        1 gtatcacacc tatgggcttc gcgatcggga cggggcggca ccggatttca aggcaaccga
       61 catcagtctg agacaatggg gcgccgagtt tcggacgtat tttcgtggaa ggaatcttgg
      121 ccccttccga ttggccgtac cgggcatcca caacgtttca aatgcgttgg tggctattgc
      181 catcggcttc gagctggaga ttcccgtcga tctgattcgc aaagggttgg cggccttcac
      241 gggagtcgag cggcgatttc atttgcgagg cgaagccggc ggtatcatgg tggtcgacga
      301 ctatggccac catcccactg aggtgaaagc gaccctcact gccgccaagc agggctggga
      361 ccgtcgatta gtggttcttt ttcagccgca tcgatacagc cggacgaggg attgcattgg
      421 agagttcgcg catgcctttg actatgccga cctgctgttc acgacggaaa tttatccggc
      481 tggtgaatca ccgataccgg gagtttctgg agccgccctg gctgaaacga tcaagtcgac
      541 gggccatcca tcggtgagct tcatcgagcg caaggaaacg ctgccggatc aggtactccc
      601 tcatctaaga ccgggcgatc tcgtcctcac gttgggcgcg ggtgatatct ggaaggctgg
      661 aaccgggatt cttgcccggc ttgagtctac ttgatggctc agaagcatcg agtgcaaacg
      721 gatggaacga aggtacgacc agtgcgggta cagcacagat tggaatccgc tgtggcgggc
      781 gttcgcgggc tggttcgctt caatgcgccg cttaaagagt acacatcgtt ccacatcggt
      841 ggtccggccg atgtgttggt ggagccggcc gatgtggaag atattgttcg tcttgcgaag
      901 cagacgcgcg aacagaaaat tccgatcttc gtgttgggcg gcaccaacat cctcattcgt
      961 gacaaaggga ttcgaggtgt ggtggtcgat ctggcaaaat tacgcgcgat caaagaggaa
     1021 ccaggatctg tgttgtatgc cgaaggcggg gtcggcatgc cgacgctgat cggccaggct
     1081 atccgtcggt cgttggccgg attggagtgg gcggccggta ttccgggcac tgttgcaggc
     1141 tgtgtcgtga tgaatgcagg gacacgactc ggtgaaatga aggactcggt gaaagccgtc
     1201 aggatcgtgt cgccaaaagg cgcattgatt gactgcccgg cagaagcaat cgaatttcga
     1261 tatcgtcggg cgacgttgcc gcagggtatt gtcgtcggag tatggttgca gctgagggtg
     1321 ggagtgcggt cggacatcga aaaggtcgtg aaaaactatc tccattaccg ccgtgatacg
     1381 cagcccctca cgctaccgag cgccggctgt gtgttcaaga atccactgaa tgattcggca
     1441 gggcgggtcg tcgaggcggc aggactcaaa ggaacatcgg tcggtgatgc acaggtttca
     1501 atcaagcatg ccaattttat cgtgaatcaa gggcatgcca gcgccgccga cgttctttcg
     1561 ttgattaaga aggtgcgcac tcgaatcgct caagagatgg ggatcaaatt ggagctggaa
     1621 ttaaaaatcg tgggtgaggt gtagcgaaag gaaatagtcg tggcgattga tcttatgaca
     1681 catgctcgga tcggtgtact catgggagga cgatcttcag agcgggatat ttcgctgaag
     1741 accggtcagg ccgtctatca ggcgctgatc cgtcgaggat acgacgcggt ggctatcgat
     1801 gtcaccgacc gtctgcatcg ggatttggag gaccacaaag tcgccattgt ctttctctcg
     1861 ttgcatggac cgggcggtga agacggaacg gttcagggat ttctcgaaac attgggaatt
     1921 ccctataccg gatccggcgt gcgcgcaagt gccgtgggaa tgcataaggt ggccacaaaa
     1981 acgatattgg ccgcgcatgg tattccgctc ccggccggaa ccgttgtgcg ggaatgcgac
     2041 aggccgtcat tggcgaaggt tctaaggcag gcaaaactga aattaccggt tgtcgtcaag
     2101 ccggtttcac agggctctac gatcggcgtc actgtcgtgc gccgagccgg ccaatggagg
     2161 gaggctctcg acctggccca tcgctatgat ccagaggtga tggtggagag ttatattccc
     2221 ggacatgaag cgacagtgtc cattctggga acggcggcgg aaggtccgaa agtacttccg
     2281 gcgattgaga tcgtggcgcc cgatggtttt tacgacttct cggcaaaata tcagaagggc
     2341 aggacccggt atctctgtcc cgctccgctc cctcccaagg tggttcacca catccgtgag
     2401 ttagccggac gaacctacga ggtattgggc tgcgagggag ccgttcgcgt agattttcgc
     2461 atcactccga gaggtcggcc gtatgtcttg gaagtcaata cggttcccgg catgacggaa
     2521 acgagtctgc tgcctatggc cgcagcccaa gcgggaattg cgtatgacga cttggtcgaa
     2581 cagatcctac agtcggcgct cgatcgcgcg agtcgctttg cacgggtggt cccaaaggag
     2641 tgagtgtgat gcgattcttg ggaagaaagc gacgatcttg tccagcgggg ccacgaaaga
     2701 atcagtggaa ggatccgcaa ggagagaggg cagtgaagga agccggccgg gcaaaaatag
     2761 ccagacggaa agcccttgtt cgaagggtcg gactggcgac cggtgcgggg ctcgtggcat
     2821 ggttcatcgt gatgggtatg cgatattccg gtcctgctct ccaaacgtta ttggagatta
     2881 ggacgatcac aatcgaagga gagcaccaca tcgataaaca gaaagtgctc gagcttgcaa
     2941 aggtgaaacc tggtacagga cttcaccata ttgtgacgag agttattaag gagcaggtgg
     3001 aatctcatcc gtgggtcaaa gaggccgaag tgacccgtgt cgcgtttcac gagttacgca
     3061 tatccctggt cgaacgaata cctgccgccg tcatccgtgc cgatgcccaa aactttctga
     3121 ctgatgcgga ggggcatgtg cttaccaagc ttggtcagat cgatgatgag gccctgcctt
     3181 tcatgaccgg aatcgattcc caaggcttgt tggatggaac tgaatcggtg agacaagtca
     3241 tcgcgtcggg catcaagctt gcaaaattgg tcgggcaaac attcgaaggc cggctgcaag
     3301 tgaacgcgga aaacccgtca aatctcgtcg cactcgtcca gggaatgcga ttccaatacg
     3361 gtgaagaagc ggtcggagag
//
LOCUS       DATNDD010000412         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_577227, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000412 DATNDD010000000
VERSION     DATNDD010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577227"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VN647_10025"
     CDS             complement(<1..635)
                     /locus_tag="VN647_10025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWG97410.1"
                     /translation="MNHRKEIDGLRAFAVLPVILFHAGFQTFSGGFVGVDIFFVISGY
                     LITSIILYEQHAGTFTLVGFYERRARRILPALFVVMVACLALGWLWLLPIDMKLFSES
                     LVAVPAFVSNILFYVTGSYFDPSAELKPLLHTWSLAVEEQYYLVYPILLLLLWRLGQK
                     WMIGLLTGLAVGSLVAAQWGSHTDPTFTFFLLPTRAWELLMGALVAFYLFQ"
     gene            1100..1717
                     /locus_tag="VN647_10030"
     CDS             1100..1717
                     /locus_tag="VN647_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HWG97411.1"
                     /translation="MRRLLVGALGFAVGYFLLTVSHGVAKETDGDVPLTGIGASFQPD
                     LFTGTLTGGMPIEVPPGRNGMQPNLALSYTSSGGNGWVGMGWKLEMGAIERQTRWGML
                     YQPTTQEELDGKVYVMNLNGMSIDLVQDATDPLLYYEKVKSAFLRIKKLSADGTAGWE
                     ITDTKGTKYRFGTGATTRMQGTVGSLGTQTLNGVWNGSKIAMATT"
     gene            1864..4325
                     /locus_tag="VN647_10035"
                     /pseudo
     CDS             1864..4325
                     /locus_tag="VN647_10035"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
     gene            4335..>4637
                     /locus_tag="VN647_10040"
     CDS             4335..>4637
                     /locus_tag="VN647_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HWG97412.1"
                     /translation="MSTNGTSTCGDLTTLSPAGTYPWYAVPCWNYQYDASGNLTRQTD
                     AKNQTLWFRYDGLNRRSQKDFTTQKAQGSGDVVYVYDDTVTTFNRKGRLKQVTDVAT"
BASE COUNT         1169 a         1285 c         1233 g          950 t
ORIGIN      
        1 ttctgaaaga gatagaacgc gaccaatgcg cccattaaaa gctcccaggc ccgtgtcggc
       61 agtaagaaga aggtgaatgt tggatccgtg tgcgaacccc actgggcggc aaccaggctc
      121 ccgaccgcta gtccagtgag caaaccaatc atccattttt gcccgagtcg ccagagcagc
      181 aacaatagga tcggatacac cagatagtat tgctcctcga cggcgaggct ccatgtgtgc
      241 agaagcggtt tgagttcggc ggacggatca aagtagctgc ccgtcacgta gaacaagata
      301 ttggaaacaa aggccgggac ggccaccaaa ctttccgaaa acagcttcat atcaattggt
      361 agcaaccaca gccagcccaa cgccaaacaa gccaccatga cgacaaacaa tgcgggaagt
      421 atccgtcttg ctctgcgctc ataaaatccc acaagggtga aggtgccggc gtgttgctca
      481 taaagaataa tagacgtaat gagatagccg ctgatcacga aaaatatgtc gacgccgacg
      541 aagccgccgc tgaatgtttg aaagccggca tgaaacagga tcactggcag caccgcaaaa
      601 gcccgaagcc catcaatttc ttttctgtgg ttcatacgct ctcagatccg ccggcaggtc
      661 ataattgatg ccatagaacg tatgttggct ttaggccgca tgcgcttgga taacattttc
      721 gcattgatgt gggatcgtcg tcctctcact gcagaaaggc tctccggaaa ccagctcttc
      781 ttcatactct ccgctctgtg ctggaagggc gtcatgttag catagatttc cgttctcact
      841 ggccctgatt ttttcggaga acgccgtcct cccccctctc tacataccgc atattttata
      901 catgtttgaa aagaccgaaa cttgccattc ccagtttgtc tgctacagtt gcaaatgtca
      961 tagtacacaa tatgatctaa atataaataa ctaagctaca tcatgatgtc gcttacatgt
     1021 ctaccggtat ccttcaacag aactcaatcg tccgtgacct ctcgctactg acaccgtatc
     1081 gattgcaaaa aggagttgta tgcgtcgtct actcgtgggt gcgctgggtt tcgcggtcgg
     1141 ctatttcctg ctcacagtca gtcacggtgt cgcgaaagaa accgatggcg atgtgccgct
     1201 gaccgggatc ggtgcgtcgt ttcaaccgga tctcttcacc ggcaccttga ccggcggcat
     1261 gccgatcgaa gttccgccag ggcgcaatgg catgcaaccg aatctggcat tgagctatac
     1321 gagcagtggg ggaaacggct gggtgggcat gggttggaaa ctggagatgg gtgcgattga
     1381 gcggcagacc cgttggggca tgctgtatca gccgacgacg caggaagaac tagatggcaa
     1441 agtgtatgtg atgaatctca atgggatgtc aatcgatctg gtgcaagatg ccaccgatcc
     1501 gctgctctat tacgagaagg tgaagagtgc ttttctgcgc atcaaaaagc tgtccgccga
     1561 tgggacggct ggttgggaga ttaccgatac caaggggacg aagtatagat tcggcactgg
     1621 agcaaccacg cggatgcaag ggacggtcgg cagtttgggc acgcagactt taaatggtgt
     1681 ttggaacggg tcgaagatcg cgatggcaac tacatgacga tgacctatac gtgggatcag
     1741 gggcagggtt atctgagtca gattgactat acgggcaacg gtgcaactgc gccgacgaat
     1801 caggtgaagt tctatctgga aaatcgtacc gatacgcccg tcatgtatac cagtcacttt
     1861 ccgatgacga cggcgaaacg gctgaagacc atcgagttat tggccaacgg agtcaggcat
     1921 cgggtctact catttgccta ctggtcagac gggacgactg cgagatcact tcttgtcagc
     1981 gcgcaacagt acggcaagga tgcgacctta gacgcttctg gcatcgtcac tggaggtact
     2041 tcgctccccc ccatccaact acgttatggt caagacatcg tgggtctgag ttccccagct
     2101 caatggatcc aatacggggg aactttccaa gcggggaaag cacagtatgc cgacgtcaat
     2161 gcggatggaa atgcagatct aatttaccaa gagtcggaca accagttctg ggtatcgctc
     2221 tcaaccggga ccggctttac agctccgacc gtttgggtcg accacggaaa tttcttcgag
     2281 ctaggacaag cccagtatgc cgatctcaat ggggatggca aggccgacct ggtcttccaa
     2341 gctacagata acaaattttg ggtctcactt tcgacgtcga cgggattcat ccccccagcc
     2401 atgtggcttc agcacggcgg cacgttccaa gcgggaatga tgcggtatgt tgatctcaac
     2461 ggggatggac gggcagacct gatcatgcaa gggacggaca acaagttttg ggtctcactc
     2521 tcaattgttc aggacactgt gcccaatacc gttcctaatc tacttcatac tataaacact
     2581 ggtctgggcg gtgccacgac cgtggcgtat actccctcgc ccaatggtca aatcagctgc
     2641 tcccctttcc cctgcagacc gtgagtagca tgaccacctg cgacaattgg aacagcagta
     2701 cctctgtgtg caatggaacc tcctcgacca caacctatag ctacaccggc gggtatcatc
     2761 acattgggga gcgagacttt cggggcttca agaccgcggt ggtgacgagt cccggggcga
     2821 cggatgccga caagaccgtg accaccacct ggtttcatca gggcaacgaa ctcacaccaa
     2881 tcgacacgga gaacccggga ctgctcaacg gctacatgaa gggcaaaccg tatcgcatcg
     2941 aggtgaagcg caaaagcgat aacctgcttt ataccaaaac cgaaacaacc tatctcgcgg
     3001 atcaaatcgg aaccgacacc acggcgccat ggtttaatcc tcctgcgcag gtcgataatt
     3061 ttctctgtga tggcggcccc tgcatcacca cccgcatgac ctttgcggcg gcggactacg
     3121 atgcctacgg caatgtgctc cgagagaatc atcagggcga catcaacgtg accggcgatg
     3181 agaagaccgt gcaacgcact tttcttgcca acaccacgga ctatgtggtg ggcgcaccaa
     3241 agcgtgaggc aatttacaaa ggcttgagca ttgcttctgc ggacaaactc gccgaaactc
     3301 tcttctacta tgacggcact ggcaccggag cctgcactgc ggcgccgacg gggagtaaca
     3361 ccgccgtaac caaaggcaag ctcacgaagg tcgagcggtg gttgaacggc gggaccaacc
     3421 caatcagcgg catggaatac gatccggcga ccggggtctt gctctgtagc cgtgatccgc
     3481 tcgggaataa gaccacactc acctacgatc caacgaaaac cttcgtcctc accagtacga
     3541 accacgtggg ccatgtcacc acaactatct attccggcgt gaatggcgtg gcgatcgatc
     3601 ctgccaccgg cttctacggc accgtcaaga gcgtgaccga tcccaatggc aagatcgtga
     3661 gtcatgagta tgatgtcttg gggcgcaaga ccaagacaac cgctcccgat ggattggtga
     3721 cgaccgtcac ctatcccacc ctcaccgagt atgggatgat cggtacgcaa aaaatcacga
     3781 cgaccacgag cggcgcgagc ctgccggcca atcttgtgag cagcaccttc ttcgatgggc
     3841 tgggccgcac gttcaaaaag gagagcagcg ggccgaatag cacgacgctc gtcactgaga
     3901 cgcaatacga tgtgaagggc caggtcttcc aaacgagtct tccgtatttc aaaaccacgg
     3961 aatccgttac gaaccgctgg cgcaccatga cctatgatcc gctcagccgt gtcacgcaag
     4021 taacccatcc cgatacgctg agcgggaatc ctctcacgag taaaagctgc tatgcctcgt
     4081 tcgtcactgt gacgctcgat ccgagtgggg cccgcaagcg ggagacgaag gatgtctatg
     4141 gccgggtggt gaagatcgaa gaatacagca ccaccacgag cacctgcgac actgccgtag
     4201 gcacgcccta tgccaccacc aattacacct atgatctgct cgggaacctg gtcaaagtga
     4261 tcgatgccct gggcaatcgc accaccatgc gatatgacag cttaagccgt aagattggga
     4321 tgtccgatcc cgacatgagc accaatggca cgagtacctg tggcgatctg accacgctca
     4381 gtcccgccgg gacctatccc tggtacgctg tgccctgctg gaactatcaa tatgatgcct
     4441 cggggaatct cactcgacag accgatgcga agaatcaaac actgtggttc cggtacgacg
     4501 ggttgaatcg ccgcagccaa aaagacttca ccacccaaaa ggctcaaggt agcggcgatg
     4561 tggtctatgt ctatgacgat acggtcacga cattcaatcg caaaggccga ctcaaacagg
     4621 tcaccgatgt cgccacg
//
LOCUS       DATNDD010000413         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4209501, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000413 DATNDD010000000
VERSION     DATNDD010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4209501"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VN647_10045"
     CDS             complement(<1..224)
                     /locus_tag="VN647_10045"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HWG97413.1"
                     /translation="MRVTIHQPQFLPWLGYLDKIDQADLFIMLDTVQFKKNEWQNRNR
                     IRTAKGWQWVTVPVLQQFGQRIDDVLINPTP"
     gene            complement(215..1822)
                     /locus_tag="VN647_10050"
     CDS             complement(215..1822)
                     /locus_tag="VN647_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97414.1"
                     /translation="MHRAGRKIVILLFVAATVLAEIAAGSAAAPAVGQYSLRAAIHVH
                     STMSTGSLSLESLARRAEEQQLDVLILSENFSLRYDYGLPPFEGFLKYHVTFPSVMEY
                     GVQRFLDEVRAAQRRHPNLIIIPGVEVAPHYYWTGSLLHGDLTMHNAQRNLLVIGLER
                     AEDYEQLPSRGNSSSFVWGRQSVVNGLPLLLLALAVWLWKPQDSRCLDWRRPAFGLRP
                     VLVIFLVTTCIVLMMNAWPLKTPVYSSHDGHVGYRPYQALIDRAVERGALVFWSMTEA
                     RDFSRYSFGPLGTITIKTEPHPESLVLTRGYTGFGGLYQDARRVIAPGGIWDQLLGSR
                     SVDDQDLFPSLIGEVAFHGTTDAGKDLDRVYTVIQTSARTPASIMAGLKGGRTYAVAR
                     GDQNILLRLDEFRLLNNGQSVGIGETLRGSGNGDIIVRVGVSAIDGKPYEARLRVIRS
                     GRIIRQIEGRTPLRIELVDREAQRGERLNYRVEVVGKGGELLTNPIYVSPMDGANTTR
                     SQRMTLPSGTVARSPIAQAESRGQTCA"
     gene            complement(1815..>2583)
                     /locus_tag="VN647_10055"
     CDS             complement(1815..>2583)
                     /locus_tag="VN647_10055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWG97415.1"
                     /translation="SIFSWIFLQIERALAWITDELIALTSAEKTEHLERAIGCADRFA
                     VVPSGIDIDRFKQARTAGKVMPEWFDCPAGATVIGSVGWLTDIKGHRFLVDAVAQLKQ
                     EHPHLHLVILGSGDRHEELLRRASKAGISQAVHLVGRREDVELALAGMDCFVLPSLNE
                     GMGRALVEAMAAGLPVIASRVGGIPALIEDEKNGLLVPAGDSLVLAAALRRILSDPIW
                     ARSLGQNAMQSIGTNYGVPAMVRAIESIYREAAVGHA"
BASE COUNT          585 a          789 c          665 g          544 t
ORIGIN      
        1 ggggtcgggt taatcagaac gtcgtcgata cgttgaccaa actgttgcaa taccggaacc
       61 gtcacccact gccatccctt ggcggtccgg atgcggttac gattctgcca ttcgttcttc
      121 ttgaactgaa ccgtatccaa cataatgaag aggtcggctt gatcgatttt gtccagatac
      181 ccgagccatg gcaggaactg cggttggtga atcgtcacgc gcatgtttgc ccccggctct
      241 cagcttgggc gatagggctc cgagcgaccg ttccagaagg aagggtcatt cgttgagatc
      301 tcgttgtgtt ggcgccgtcc atcggtgaga catagatcgg attggtcagc aattcccccc
      361 ccttgccgac gacttccacc cgataattca gtcgctctcc tcgctgtgcc tcacgatcca
      421 caagttctat ccgcaacggc gtccggcctt cgatctgtct gatgatccga ccggatcgaa
      481 taacgcgaag cctcgcttca tacggctttc catcgatggc ggacacgccg acacggacga
      541 tgatgtcgcc gttcccactt ccacgaagcg tctccccgat ccccacggac tgcccgttgt
      601 tcaacaggcg aaactcgtcg agccgaagca gaatattctg gtcacctctc gcaaccgcat
      661 aggttcgtcc tcccttaagc ccggccatga tggaggcagg agttcgcgcg gatgtctgga
      721 tgaccgtata cacccgatcc aaatcctttc cggcatccgt cgttccatgg aatgccacct
      781 ctccgatcag tgaggggaac aggtcctgat cgtctaccga ccttgatccc agcagttgat
      841 cccatatgcc acccggcgcg atcactcgcc tggcgtcttg gtacaacccg ccgaatcctg
      901 tatagcctcg tgtcaagacc aaggattcgg ggtgtggttc tgtcttaatc gtgatagttc
      961 ctagtggacc gaatgaatag cggctgaaat cgcgagcctc cgtcatcgac caaaacacca
     1021 atgcgccccg ttcaaccgcc cgatcaatca aggcttgata aggacggtag ccgacgtgtc
     1081 cgtcatgcga gctgtaaaca ggcgtcttca acggccacgc attcatcatc agaacaatac
     1141 aggtagtcac gaggaagatg acgagcacag gccggagacc gaatgcaggc cttctccaat
     1201 caagacatcg cgagtcttga ggtttccaca accagacggc gagagccaac agcaacagtg
     1261 ggagtccatt gaccacactc tgccggcccc atacaaacga ggaagaattt ccccgagaag
     1321 gaagctgctc ataatcctcg gcacgttcca gcccgatgac caggaggttc cgctgggcgt
     1381 tgtgcatcgt gagatccccg tgcagcagcg aaccggtcca gtagtaatgc ggggcgacct
     1441 cgaccccggg aataataatc agattcgggt ggcggcgctg cgctgcgcgc acctcatcca
     1501 gaaatcgttg gacgccgtat tccatgacgg aggggaacgt gacatggtac ttgaggaatc
     1561 cttcgaacgg cgggagtcca tagtcatacc gcaaagagaa attttcggag agaatgagga
     1621 catccagctg ttgctcttcg gctcttctgg ccaacgattc caagctgaga gatcctgtac
     1681 tcatcgtact gtgtacatga atcgcggctc gcagtgagta ttgtcctaca gctggtgcag
     1741 cagctgaacc tgctgcgatt tccgccagca ccgtcgcggc aacaaacagt aggataacaa
     1801 ttttacggcc ggctctatgc atgacccacg gctgcttccc tgtaaattga ttcaatggcc
     1861 ctgaccatgg cgggaactcc gtagtttgtt ccgatgctct gcatcgcgtt ctgtcctaat
     1921 gaacgtgccc atataggatc ggacaatatc cgtcgcaacg cggcggccag cacgagactg
     1981 tctccggctg gaacaagaag gccgtttttc tcatcttcaa tcagagcagg aatccccccc
     2041 actcggctgg caatcaccgg aagtcccgct gccatcgcct caaccaaggc cctccccatc
     2101 ccctcattga gtgacggcaa gacgaaacaa tccatcccgg ccagggcaag ttcgacatct
     2161 tctcgacgac ccaccaaatg cacggcctga ctgattccgg ctttacttgc cctgcgcagc
     2221 aactcctcat gtcgatcccc actgccgaga atgacgagat gcagatgagg atgctcctgc
     2281 ttcaattgag cgacggcgtc gaccaaaaat cgatgcccct taatatccgt caaccagccg
     2341 accgagccga tgacggtcgc gccagccggg caatcgaacc actccggcat cacctttccg
     2401 gccgtccgag cctgtttgaa acggtcgatg tcgataccgc ttggtacgac ggcaaaccga
     2461 tccgcgcacc cgatggctcg ctcaagatgc tcggtctttt cggccgatgt cagcgcgatc
     2521 aactcatcgg tgatccatgc cagcgcgcgc tcgatctgta aaaatatcca ggagaagatc
     2581 gaa
//
LOCUS       DATNDD010000414         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_373922, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000414 DATNDD010000000
VERSION     DATNDD010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373922"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..749)
                     /locus_tag="VN647_10060"
     CDS             complement(138..749)
                     /locus_tag="VN647_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97416.1"
                     /translation="MSQADIDQFVHIRRMRDSRFPHEITSILPGEFFVSGDPMIVYTV
                     LGSCISACVRDPIAGVGGMNHFMLPKPKERGCDSWGESTRYGSYAMESLINGILKRGG
                     IKSRLEVKLFGAGKIYDGNIDVGARNTEWVLEYLKAEGLSACKTDLGDMYPRKVYYFT
                     ESGRVLLKKLERLKNRTIEIREQEYAAKIQTREQAAENEVTLF"
     gene            complement(755..1573)
                     /locus_tag="VN647_10065"
     CDS             complement(755..1573)
                     /locus_tag="VN647_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HWG97417.1"
                     /translation="MDYKITSKEFECFRTLIYGESGISLTQQKQSLLESRLSKRLREL
                     GIETFSQYYDKVTGDRTREEFTRMLDLISTNKTDFFREPKHFDFLREVILPELIPEKR
                     IRIWSSACSTGEEPYTIAITLFENVQNPSEWDFKILASDLSTRVLAKAASGIYDEERV
                     RDMPAGMLRRHFLRGCRDSMGSFKVKPHLASMIRFRRLNLMDDDFPIKSPLDLIFCRN
                     VMIYFDRPTQETLVNKFYRYLKPGGYLFIGHSESLQWINHPFRSLAPTIYQRER"
     gene            complement(2237..>3168)
                     /locus_tag="VN647_10070"
     CDS             complement(2237..>3168)
                     /locus_tag="VN647_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWG97418.1"
                     /translation="AEQITRGNEDLSQRTSEQASSLEETSSAMEEMTSTVKQNADNAK
                     QANQLAIAARDVANKGGAVTTKAVEAMGAINQSSKKIADIITVIDEIAFQTNLLALNA
                     AVEAARAGEHGRGFAVVATEVRNLAQRSATAAKEIKELINESIQRVMDGSELVNQSGK
                     TLEDIVGSVKRVTDIIAEITAASQEQASGIDQVNKTIMSMDETTQQNAALVEQTTSAG
                     QSMKEQAKELMRQVEVFKIRRSESRCTEEPTKRDSAPADKQPTTPAASAHNLKGMKRS
                     SILPRSVEQHQPVGLTAGNGKNYRNKGDDFEEF"
BASE COUNT          667 a          900 c          790 g          811 t
ORIGIN      
        1 tggcgagcac ttgccgcatc aacgccgagt catctaccgt caagacgcga atcttgttca
       61 tacatctctc acattacgcg cgacaaagta ttgcacccac agcattgacg cgccttacac
      121 cctaccggca ccacgttcta aaacaacgtg acttcattct ccgccgcctg ttcacgtgtt
      181 tgaatcttgg cggcatactc ttgctcacgg atctcgatcg ttcggttctt aagtcgctca
      241 agttttttca acagcaccct acccgactcg gtgaagtagt acactttgcg cggatacata
      301 tcgcccaagt cggtcttaca ggcggatagt ccttcggcct tgagatactc caagacccat
      361 tcggtatttc tggcgccgac atcgatgttg ccgtcataaa ttttcccggc gccgaacagt
      421 ttgacctcta agcgactctt gattcctcct cgcttgagga taccgttgat caacgactcc
      481 atggcatagg acccatatcg ggttgattct ccccatgagt cgcagcctct ttctttcggt
      541 ttcggcaaca taaaatgatt catcccgccc acgccggcaa tcggatcacg cacacaggcg
      601 gagatgcatg aaccgagaac ggtgtacacg atcataggat cgccgctcac aaaaaattcg
      661 cccggaagga tggaggtgat ttcatgaggg aatcgactgt cacgcatccg tcgaatatgg
      721 acaaattggt cgatgtcagc ctgtgacatg tgtgtcatcg ttccctttga tagatcgtcg
      781 gagccaagga ccggaaagga tgattgatcc attgaaggct ttcagaatgt ccaatgaata
      841 aatacccacc cggcttcagg tagcggtaaa atttgttgac aagggtttct tgagtcggac
      901 gatcaaaata gatcatcacg tttcggcaga agatcaaatc aagcgggctt ttgataggga
      961 aatcatcgtc catcaaattg agtcgccgaa agcggatcat ggaagccaag tgtggtttga
     1021 ccttgaagct acccatgcta tcgcggcatc cgcgaagaaa atgccgcctc aacatgcccg
     1081 caggcatgtc tcgaactcgc tcttcatcgt agatacccga tgccgctttc gccaaaacac
     1141 gtgtcgacag gtcggacgcc agaatcttaa aatcccattc cgatgggttc tgaacgttct
     1201 caaaaagcgt gatcgcaatc gtgtagggtt cttccccggt cgaacaagcg gacgaccaaa
     1261 tacgaatccg tttttctgga atcagctccg gcaatattac ttcgcggaga aaatcaaagt
     1321 gtttgggctc acggaaaaaa tcggtcttgt tggtcgaaat caagtccagc atccgcgtga
     1381 actcttcccg cgtacgatct ccggtcacct tgtcgtaata ctgcgaaaat gtctcgatcc
     1441 cgagttctcg caggcgtttg gacagcctgg attccagtaa actttgcttt tgctgagtga
     1501 gcgagattcc gctctcgcca tagataagcg tgcgaaaaca ttcgaattct tttgaggtga
     1561 ttttgtaatc catcaggcat gccctttact gcccagtaca ttcaggccgc aagacgctcg
     1621 gatgcctgct cccagtagtg ctggagtctt ttcggttgtt tgaggaaatt cttgatgatc
     1681 ggcgcactcg ccgtcggaca catcggtaga aaagtcgata cagagaagaa tcgacggtga
     1741 ctgcagggat accgcttgtc cccgggttgc accaggctga tagcggtcga tgaatatcct
     1801 gcgaagcgct tcaggcggcc gcttgtcaac cggatgcctt gggcccagat gacatttgag
     1861 ccgtgagcgc catctctaat tgcgccttcg tcacaccgcc gttccctttg tatgccgaat
     1921 agagttgtac aaaacgatta gcccccgacc atggaagcac tgtcacacag ttccatgagc
     1981 agctttttca ggctctctga aacgggctca tccgaatcgc tctgttccta cgccactgcc
     2041 ttcatggcgg cgcttacttg attggaaaac attgtgacaa gttcatcata ttcccccggc
     2101 gatacattca attttcctac gatgataagc ctctgaccag tgacttcggt tagacttggg
     2161 cctgtcggcg tagatccagg ccgatagctt cggcgagtcg ttcactttcc ttgttgaccg
     2221 atcgacaaga cgcatcctag aattcctcga agtcgtcccc cttgttgcga tagttcttgc
     2281 cgttgcccgc cgtgagaccg accggttgat gttgctcaac tgatcgggga aggatcgacg
     2341 accgcttcat gcccttcaga ttgtgcgcgc tcgccgccgg tgtggtcggc tgtttgtcgg
     2401 ccggcgcgct gtctctcttc gtcggctctt cggtgcaccg actctcggag cgccggattt
     2461 tgaacacttc cacttgccgc atcaactctt tcgcctgctc cttcatggac tgaccggcgc
     2521 tggtagtttg ttccaccaag gcggcattct gctgcgtagt ctcgtccatc gacataatgg
     2581 tcttgttcac ctgatcgatc ccgctcgcct gctcctgaga ggccgccgtg atctccgcaa
     2641 tgatatccgt cacacgcttg acggaaccga caatgtcttc cagagtcttg ccggattgat
     2701 tgaccagttc gctgccgtcc atgacgcgct gaatagattc gttgatcaat tctttgattt
     2761 ctttggcggc cgtagccgat cgttgagcca gattccgcac ttcggtcgcc accacggcaa
     2821 atccccggcc atgttcgccc gcccgcgccg cttccaccgc cgcgttcaat gccaaaagat
     2881 tggtctggaa ggcgatttcg tcgatgacgg tgatgatgtc ggcgatcttc ttgctgctct
     2941 ggttgatcgc gcccatcgcc tcgacggctt tggtggtcac cgcgccgccc ttgttggcca
     3001 cgtcgcgagc tgcaatcgcc agctggttcg cctgtttcgc attgtcggca ttttgcttca
     3061 ccgtggaggt catctcttcc atggcactgg aggtttcttc cagcgaggag gcctgctcgc
     3121 tggtccgttg cgagaggtcc tcgttgcccc tggtgatctg ctcagcac
//
LOCUS       DATNDD010000415         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3629067, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000415 DATNDD010000000
VERSION     DATNDD010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629067"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VN647_10075"
     CDS             complement(<1..318)
                     /locus_tag="VN647_10075"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWG97419.1"
                     /translation="MAKRAVLIGINKYQVPGADLQGCVNDVKNLSGALKTYYGFIDKD
                     IAMLTDAQATKKAMERAIKKLIASGKKGDVLLLHYSGHGSNVPDDNGDEADHRDEILC
                     PTDL"
     gene            complement(354..905)
                     /locus_tag="VN647_10080"
     CDS             complement(354..905)
                     /locus_tag="VN647_10080"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HWG97420.1"
                     /translation="MNNLNKTTAHEHMRVEMDKLIASLDLPELNKEFMRVRWLELVIW
                     MDGKAQESVWWYRRLRLATIIGGVIVPALVSLNVGDDVAPLVKTLTFIVSLAVALSAA
                     VEEFFRYGERWRHYRRVTESLKSEGWQFLQLSGTYADHTHVQGYPAFAARVEELNREE
                     VESYITTVFTEKKEADEKTPSVP"
     gene            complement(1214..1858)
                     /locus_tag="VN647_10085"
     CDS             complement(1214..1858)
                     /locus_tag="VN647_10085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG97421.1"
                     /translation="MNQLIPSEIEAYAETHSIPESSVCRALREETYRTMEYSRMLVGP
                     LEGAFLKMMTQVVGAKRVLEIGTFTGYSALCFAEALPKDGTVVTCDIDEKAAAVARRY
                     IAQAPFGRKIDIRMGPALETMRTLSGLFDLIFIDADKTNYLNYYRRSLDLLVPNGVIL
                     IDNVLWSGEVLKQPPPDESTAAIQELNRTISADPRVTAVLVTIRDGILVVRKAN"
     gene            1961..>2575
                     /locus_tag="VN647_10090"
     CDS             1961..>2575
                     /locus_tag="VN647_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HWG97422.1"
                     /translation="MKTFATLEPLTTRLLEIQRINSAASVLSWDQETYMPAGGGEARA
                     EQIAVLQGIAHQKLVSPDVQALLSQWVDPATGQAADQPGETWDEPSRSLLREVWRDFS
                     RAKKLPSDFVVKLSRECSLAQQVWVEAKEQNTFVMFLPNLRTVLQLKREEAEYLGYHD
                     SPYNALLDVYEPGSTIAGLQPVFAALKARLVPLLKRIIESRVQID"
BASE COUNT          551 a          743 c          688 g          593 t
ORIGIN      
        1 cagatcggtc ggacacagaa tttcatcccg atgatcggct tcgtcgccgt tgtcgtccgg
       61 cacgttcgac ccatgcccgg aataatggag gagcagcaca tcgcccttct tcccgctcgc
      121 aatcagtttc ttgatcgctc gttccatcgc ctttttcgtc gcctgggcat ccgtaagcat
      181 cgcgatgtcc ttatcgataa agccgtaata ggttttcagc gctccgctca gattcttcac
      241 gtcattgaca catccttgga gatcagcccc cggcacctga tacttgttga tcccgatgag
      301 caccgctcgt ttggccatac gcactccttt ctgtgattga ataggtaccg acttcacggc
      361 acagagggtg ttttttcatc tgcctccttt ttttctgtga acaccgtcgt gatgtaggac
      421 tcgacctcct cacggttcag ttcttcaacc cgcgccgcga aggcggggta tccttgaaca
      481 tgtgtatggt cggcataggt tccgctcagt tgtaaaaact gccagccttc gctcttgagc
      541 gactcggtca cccggcggta gtgccgccat cgctcgccgt atcgaaagaa ctcctccacg
      601 gcggcgctta acgccaccgc aagactgacg atgaacgtga gcgttttgac aagaggtgcg
      661 acgtcatcgc cgacattcag gctgaccagc gccggtacga tcacgccgcc tatgatcgtc
      721 gccagacgca gtctgcgata ccaccacacg gactcctgtg ccttcccatc catccaaatg
      781 accagctcca accatcgcac ccgcataaac tctttgttca gttccggcaa atccagtgat
      841 gcgatgagct tgtccatctc gacccgcatg tgttcgtggg cagtcgtctt attcagatta
      901 ttcataggct cctccatcga gtcgttcacg gcgagagaaa ggccacgtgg ttcccacggc
      961 ctgcgttttt caagcccaaa gagtacatgt ccgacagaaa cgcagctttt tcatacctgc
     1021 aatttgcaca agccctgaac cgacaagaaa ggagctatgc tgggctgaat tagggaagcg
     1081 gcgatgaagg tgtgtcgcca taacggggta tcaaacgaga tgcagcgtat gccagtatag
     1141 ataccataag tagagccggt cacgaaacca gcgtttgttc gatcgttcct cccttaacct
     1201 gtgctttcaa gttctaattc gccttcctca ccaccaaaat cccatcccgg atcgtgacca
     1261 gtacggcggt cacgcgcgga tcggctgaga tggtacggtt cagctcttga atggcggcgg
     1321 tggactcgtc aggcggcggc tgtttcaaga cttcgccgct ccacagcacg ttatcgatca
     1381 agatcacgcc gttcggtaca agcaggtcga gcgaacggcg gtaataattg aggtagttgg
     1441 ttttgtctgc atcgatgaag atcagatcaa agaggccgct cagtgtcctc atggtctcaa
     1501 gggccggtcc cattcggata tcgatctttc ttccaaacgg cgcttgcgcg atatagcgcc
     1561 tggccaccgc cgccgctttc tcatcgatat cgcaggtgac tacggttccg tctttgggta
     1621 gggcttcagc aaagcagaga gcactgtaac cggtaaacgt cccgatttcg agcacgcgct
     1681 tcgcacccac cacctgcgtc atcattttca ggaacgcccc ttccaaggga ccgaccagca
     1741 tccgggagta ctccatggtc cgataggttt cttcgcgcaa tgcgcggcag accgacgact
     1801 cagggatgga atgggtttcg gcgtatgctt cgatctcgga cgggatcagc tggttcatct
     1861 gtattccttt cagcaatttg aaaagactga tgtggttgcg tacactgcgc atgattgccg
     1921 aatcatgagc gaatcttagc atagtggaaa ggagaccgga ttgaagacgt tcgcgacgtt
     1981 ggaaccgctt acgaccaggt tgctggaaat tcaacgcatc aatagtgccg cctcggtctt
     2041 gtcgtgggat caggaaactt atatgccggc cggcgggggg gaagcgcgag ccgagcagat
     2101 cgccgtgctt caaggcatcg ctcatcaaaa gttggtctca ccggacgtgc aggcgctctt
     2161 gtcgcaatgg gtcgatcctg caaccgggca agcagccgac caacccggtg agacgtggga
     2221 tgagccgtca cgctcgctct tgcgcgaagt gtggcgggat ttcagccgcg cgaagaaact
     2281 gccgtcggac ttcgttgtga agctgagccg tgaatgctcg ctggcccagc aagtgtgggt
     2341 cgaggcgaaa gaacaaaata cattcgtgat gtttctgccc aacctccgga cggtgctgca
     2401 gctcaagcgc gaagaagccg agtatctcgg ataccacgat tcgccctaca acgccctgtt
     2461 ggacgtctac gaaccgggct cgaccattgc cggcctccag ccggtgtttg ccgcactcaa
     2521 ggcgcgtctc gtgcccttgc tgaaacgaat cattgagagc cgcgttcaaa tcgat
//
LOCUS       DATNDD010000416         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3553122, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000416 DATNDD010000000
VERSION     DATNDD010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3553122"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VN647_10095"
     CDS             <1..532
                     /locus_tag="VN647_10095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWG97423.1"
                     /translation="KRAEERLTYLAQYDQLTGLVNRTLFRDRLIQAMARSKRVQQPLG
                     LMLLDLDRFKPVNDTMGHNVGDQVLKAVAERLQECVREVDTVARMGGDEFTIILEGLT
                     CEEDITLVARRITKSLAEPFHLGEHTALIGVSIGITIYPTDDHDVDELLKHADAAMYR
                     AKQQGGGSFQFHIRLS"
     gene            complement(567..1358)
                     /locus_tag="VN647_10100"
     CDS             complement(567..1358)
                     /locus_tag="VN647_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWG97424.1"
                     /translation="MSKEVIPANPLGGRTLVVDPVDRHCYATPSAALKDVGESDQVYV
                     RPGIYADKLVVTQRPVRLVGAGRDHVQIFCRRGGPLYLQEVPEGWITGITFRYVGSDQ
                     HSALNILNSTCIITQCRAMEGILSGAVLYGPECRVAFTDNDVCRNRESGIFVFAGAQP
                     RVADNRCVENHHFGIAVRDPGSRPELVRNLCEENMLSGMLLFQHAEGLIVDNVCRNNQ
                     QWGILMTPDSRPNPAPSALPTMNRLEPNPMGAYSISDQPLAEIGR"
     gene            1635..2126
                     /gene="ybgF"
                     /locus_tag="VN647_10105"
     CDS             1635..2126
                     /gene="ybgF"
                     /locus_tag="VN647_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HWG97425.1"
                     /translation="MLRRCIRILMLLFIFFPVSQSLAAPPQRQDTTRRLYDRVMDEFK
                     HRDYEAAMAGFRLFIELHSQSALAANAQYWIGECQYRTRRYRDALKSFYDVVSNYPLS
                     PKLSASTLKLGQTYTKLGDHEKARLMFDRVVEEYPESPEAEVARKAVEAIPSAEDATS
                     QSP"
BASE COUNT          497 a          594 c          567 g          470 t
ORIGIN      
        1 aaagcgggct gaagagcggc tgacctacct tgcccaatat gatcagctga ccggcctggt
       61 gaaccgcacc ctgtttcgtg atcgccttat ccaagccatg gcgcgcagca aacgagtcca
      121 gcagcctctc ggtctcatgc tgctcgacct tgatcgattc aaaccggtga acgataccat
      181 gggtcacaat gtcggtgacc aggtgttgaa ggccgtcgcg gaacgacttc aagagtgtgt
      241 gcgcgaagtg gacacggtcg cccgcatggg aggagatgag ttcacgatta tcctggaagg
      301 cctgacgtgc gaagaagata tcacgcttgt tgcacggcga atcacgaagt cattggcgga
      361 accattccac cttggagagc acacggcgct gatcggagtc agtatcggta ttaccatcta
      421 ccccacggat gatcatgacg tcgatgagct cctgaagcat gccgatgccg ccatgtatcg
      481 ggccaaacag caagggggag gctcttttca gtttcatatc cgcttgagtt agcaatcatc
      541 tacccgtcag tgacccccgg ccgccatcat cgaccgatct cagcaagcgg ttgatccgag
      601 attgaatacg cacccatagg attcggttcg agtcgattca ttgtaggaag cgccgatggc
      661 gccggattgg gacgcgaatc gggtgtcatg aggatccccc actgctgatt gttccgacag
      721 acattatcga cgatcaaccc ttcggcgtgt tggaacaaga gcatgccgct cagcatgttt
      781 tcttcacaca gattccgcac cagttccgga cgactgcccg gatcccggac cgcgatgccg
      841 aaatggtgat tctccacaca gcgattgtcg gccacccgcg gttgagcacc cgcaaagacg
      901 aaaatgcccg actcccgatt gcggcacacg tcgttgtcgg tgaacgcgac tcgacattcg
      961 ggcccatata acaccgctcc cgacaagatt ccttccatcg ctcggcattg cgtgataatg
     1021 catgtagagt tgagtatgtt gagagccgaa tgttgatcgc tgccgacata acggaatgtg
     1081 atgccggtta tccatccttc cgggacctcc tgtagataca gcggcccgcc gcgccggcag
     1141 aagatttgta cgtgatctcg tccggcaccg acaagccgta ccggtcgttg agtgacgacg
     1201 agcttatccg catagatccc gggacggaca tagacttggt ccgattcacc gacatccttc
     1261 aacgccgcac ttggagtcgc ataacagtgc cgatcgacag gatcgacaac caatgttcgt
     1321 ccgcccaaag ggttggccgg aatgacttct ttactcacga tgctctcctc aggagaggtt
     1381 tgctgctttc tttgcggcct tggagagaag agagctcatt gaatactgaa ttcactgcta
     1441 cagagattcg aaccactttg tacaaatctg tgtcttcctt tacacgccct tgaaaaggct
     1501 cgagaccgac ggattttgga atcgaacaaa attctatttt ttcagcggtt ctcgtgcagg
     1561 aatgttcgtg gctctggcat accgattgcg tatgtaagcc cctcgactgc cctctaagaa
     1621 gatggaggct cgatatgctc cgaagatgta tcaggattct catgctccta tttattttct
     1681 tcccggtcag ccagtccttg gccgcccctc ctcaacggca agacacgacc cgccgattat
     1741 acgatcgcgt catggatgaa tttaagcacc gtgactatga ggcagcgatg gcagggttcc
     1801 gactcttcat cgagctgcat agccagtccg ccttagcagc caacgcgcaa tattggatcg
     1861 gagaatgtca gtatcgaacg agacgatatc gggacgcgct caaatcgttc tatgacgtcg
     1921 tgtccaatta cccgctcagc cctaaattgt cggcgtccac gctgaagctc ggtcagacgt
     1981 acacgaaact gggagatcac gagaaggcgc ggctcatgtt tgaccgagtt gtggaggaat
     2041 acccggagag cccggaagca gaagtcgctc gcaaagccgt ggaagctatc ccaagtgccg
     2101 aagacgcaac gagtcaatcg ccgtagta
//
LOCUS       DATNDD010000417         5738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_194742, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000417 DATNDD010000000
VERSION     DATNDD010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194742"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VN647_10110"
     CDS             complement(<1..840)
                     /locus_tag="VN647_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWG97426.1"
                     /translation="MIISAAAMAKHKHEEHENHERWLVSYVDFITLLFAFFVVMYSIS
                     SVNIGKYRTVSESIKAALNPLANQSGANQIISVGEAKMALQGRNPTDAKEVTIRRIRQ
                     LAQVIKDKQLKDMVAFVTVVQTINGDIVITIPDRLLFNSGEATVKTEALPFLEGLGGV
                     ILEMNRHARVEGHTDDVPIRTAQFPSNWELSAARAVMVVRVLSELYGVPADHLAAAGH
                     ADTRPVTSNSDAEQRAKNRRVEVVILEQAPAAPILQPGNESEASEQSAEDMPKNPSQL
                     GPES"
     gene            complement(906..1676)
                     /locus_tag="VN647_10115"
     CDS             complement(906..1676)
                     /locus_tag="VN647_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HWG97427.1"
                     /translation="MDIATILGMVIALGSILGGQVLEGGHLSSILQLTAFIIVIGGTV
                     GAICIQNPLPVVIKAIGALSLALSGPRIDNKGTIKLILDLANLSRKQGLLALEGKLKD
                     INDPFMKKGIQLIVDGTEPKVVHEILEIEVEHHEEQGVIAAKVWEAAGGYAPTVGILG
                     AVLGLIHVMENLADPSKLGSGIAVAFVATIYGVGAANLFFLPLANKIKLKLKEEAGGR
                     NLIIMGLVGLAHGENPRLLQEKLESVLPRNERIKEAKK"
     gene            complement(1689..2276)
                     /locus_tag="VN647_10120"
     CDS             complement(1689..2276)
                     /locus_tag="VN647_10120"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HWG97428.1"
                     /translation="MHNQEHKLYGELGALARFIDQAMKAISAAGPQIMSSSTQLPAAA
                     SHLSDLSKMTEDGTLEVMRLTEMIQDNHGRIAKELSGIVEVLRAMDCASLAGRLGNVT
                     SVLVQEGKCLTEIMTALSFQDLVAQRVKKLVAILDEVQGKLMELIVVFGLQESCETAR
                     DAGTAGYLLKQLEESKTTAMKQKVADDILAQFGFK"
     gene            complement(2323..2703)
                     /locus_tag="VN647_10125"
     CDS             complement(2323..2703)
                     /locus_tag="VN647_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HWG97429.1"
                     /translation="MPADPNMKILVVDDMVTMRRIVKNILKQLGFVNVEEAENGQEAL
                     QKLQADTYGFVVSDWNMPVMTGIDMLRAIRVDEKLKTTPVLMVTAEAQQSNLVEAVQA
                     GVSNYIVKPFTAETLQEKIVKIFK"
     gene            complement(2719..3060)
                     /locus_tag="VN647_10130"
     CDS             complement(2719..3060)
                     /locus_tag="VN647_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97430.1"
                     /translation="MNGCMVDPGKASDLRVGRCLAHRQNDSIVALSGGKSLGLERHGP
                     SDDDPPGTSFFHARRSFPTHAAKSTTHRTIPAQVDRRMCRYPTKRREAVERSDFPIMD
                     KTSAIVAVTGG"
     gene            3430..4329
                     /locus_tag="VN647_10135"
     CDS             3430..4329
                     /locus_tag="VN647_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWG97431.1"
                     /translation="MAHVISVASGKGGVGKSVVSVNLALVLARKEKQVVLADLDVGGA
                     DAHVLVGLLNPPLTLTDFLNHRIERLDDLAQGLSIHPNLRIIPGTGDTLATANMAYSQ
                     KKRLIRNFQDIRADVIIVDIGAGTGYHALDFFLMADHHLAVATPDPTSVLDLYRFIKL
                     AAIRRVLSMFVMRDVMAERLANRDYSSVEEVLDVAGKTDESGRSIAESTLKAFQPALI
                     LNRVSGRARVNVPQLKKLLKEYVGGDLTLLGEIPEDHAMEQAVRAYVPVVQHTPSSPA
                     AVALAKTADALARLLHDAPPQAA"
     gene            complement(4453..5463)
                     /locus_tag="VN647_10140"
     CDS             complement(4453..5463)
                     /locus_tag="VN647_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWG97432.1"
                     /translation="MNQLNIKEIIGNIEKRIDDHRLVNARITSEINILSLNATIEAAR
                     AGDAGKGFAVVATEVKHLAAQSADASKALSSIREETSELNRRFMDKEHDRLSEMAQTL
                     VQLIVRNLYERTADVRWWAMDEALVKGLESLDRAALQHAVGRLGLINRYYSVYLNLVL
                     VGIDGKIMACSHPEKFPRIIGADTMRVSWFQKAMATTNGDQYVVDDICYDPLHGDKLV
                     AVYAAAVRKGGNTNGRIIGVLGVVFDWEEQSGTIVQKEPAISEASWRRTRVMLLDNRL
                     RVIAASDHQGLLVPFTLQHQGRQRGHYVNDKDEVIAYAKSLGYQEYDGLGWYAVIVQK
                     LR"
     gene            complement(5549..>5738)
                     /locus_tag="VN647_10145"
     CDS             complement(5549..>5738)
                     /locus_tag="VN647_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97433.1"
                     /translation="LPPFTPIHAVTQVIRSTPDSQRQGWVLATEFLEISSDDLDHLIR
                     HILHAQAERLRARRNHPG"
BASE COUNT         1189 a         1655 c         1472 g         1422 t
ORIGIN      
        1 cgactccgga cccagctgcg atggattttt cggcatatct tccgccgatt gctcggatgc
       61 ctctgattca ttgccgggct gaagaatcgg agcggcggga gcctgttcga gaatgacgac
      121 ttcgactcga cgattcttgg ccctttgttc ggcatcagaa ttggacgtca caggcctggt
      181 atcagcatgc cccgcggccg ccaggtgatc tgccggtact ccgtagagtt cagacaacac
      241 ccgcacaacc atcaccgccc gtgccgccga caattcccaa ttggacggaa attgtgccgt
      301 ccggatggga acgtcatccg tatgcccttc gacgcgggca tgccggttca tttccaggat
      361 aactccccct aatccttcga gaaaaggaag agcttccgtt ttgacggtcg cttctccact
      421 gttaaacagt agtcgatcgg ggattgtaat gacgatgtct ccattgatcg tctgcacgac
      481 ggtgacgaac gccaccatgt ctttcagttg cttatcttta atgacttggg ctaattggcg
      541 gatacggcga attgtgactt ccttggcatc tgttggattc ctcccctgta gcgccatttt
      601 tgcttcgccg acgctgatta tctggttcgc acccgactgg ttggcgagtg gattcagcgc
      661 cgccttgatc gattcgctga cggttcgata ctttccgatg ttgaccgagg agatcgagta
      721 catgacgacg aagaacgcaa acagcaaggt aatgaaatcg acataggaga ccagccatcg
      781 ctcgtgattt tcgtgttctt cgtgtttatg cttggccatg gcagcagcgc taatgatcac
      841 ccgtcatcgg tcattcatcg ttcgccattt tttcaatgac cgttgacgga tgactgatga
      901 cctctttact tcttcgcttc cttgatccgt tcattacgcg gaaggacgct ctccaacttt
      961 tcttgcagca accgaggatt ttcaccatgc gcgagtccga cgagacccat gatgatcagg
     1021 ttgcggcctc ccgcttcctc tttcaatttc agcttaatct tgttggcaag ggggagaaag
     1081 aagaggttcg ccgctcccac gccgtagatc gtggcgacga atgcgaccgc gatcccgctg
     1141 ccgagcttcg agggatcggc gagattttcc atcacgtgaa tcaaccccag taccgcgccg
     1201 aggatgccga cggtcggcgc gtaccccccg gccgcttccc agaccttggc cgctattacg
     1261 ccttgctctt catggtgctc cacttcaatc tcaaggatct catgcacgac tttcggctca
     1321 gtgccgtcga cgatcaactg gatccctttc ttcatgaagg gatcgttgat gtctttcagt
     1381 ttcccctcga gcgccaacaa cccttgcttt cgcgagagat tggcgagatc caagatcagc
     1441 ttgatcgttc ccttattgtc gatgcgagga ccggaaagcg ccagagatag ggcgccgatc
     1501 gccttgatga cgaccggcag cggattttgg atgcagatcg ctccgaccgt tcctccgatc
     1561 acgatgataa aggcggtcag ctgcaagatc gaactcaggt ggccgccttc gagcacctgc
     1621 cccccgagga tggagcccaa tgcgatcacc atcccgagta ttgttgcaat atccattgta
     1681 cggacacctc atttgaaccc gaattgggcc aagatgtcgt cggcgacctt ctgcttcatg
     1741 gctgtcgttt tcgattcctc gagttgtttc aataagtacc ctgctgtacc tgcgtcacgt
     1801 gccgtctcgc agctttcttg aaggccgaac acgacaatga gctccatcaa cttcccttgc
     1861 acttcgtcga gaattgcaac gagtttcttt acacgttgag ccaccagatc ttgaaaggat
     1921 agagccgtca tgatctccgt cagacatttg ccttcctgca cgagaacgga ggtcacgttc
     1981 ccgagacgtc ccgcaaggct cgcgcaatcc atcgcccgca gaacctcgac gatccccgag
     2041 agctccttgg cgattctccc gtgattatcc tgaatcatct ccgtcaaacg catgacttca
     2101 agcgtgccat cctccgtcat cttgctcagg tcactcaaat gggaggcagc ggccggaagc
     2161 tgagtactgc tggacatgat ctgagggccg gccgccgaga tggctttcat cgcttggtcg
     2221 ataaaacggg ccaacgcgcc gagttcaccg tataatttat gttcttggtt gtgcacatcc
     2281 cctctctatt ccggtgtaag cacggatctg ctcgagcacg ggttacttga aaatcttcac
     2341 gatcttttct tgcaaggtct cggcagtaaa cggcttgacg atgtagttgc tgacacctgc
     2401 ttgcaccgcc tcgaccaagt tgctttgttg cgcctcggcc gtcaccatca agacgggggt
     2461 tgttttgagc ttctcgtcca ctcggatcgc acggagcatg tcgatgcccg tcatgaccgg
     2521 catgttccaa tcggacacga cgaagccgta ggtatcagcc tgcagcttct gcagtgcttc
     2581 ctgtccgttt tccgcttctt ccacgttcac gaatcccaat tgtttcagaa tattcttgac
     2641 gattcttctc atcgtcacca tgtcatcgac cacaaggatt ttcatgttcg gatcggctgg
     2701 catagcgcct cctttttctt accctcctgt tacagcgacg atggccgacg tcttgtccat
     2761 gatgggaaag tccgaccgtt ccacggcctc tcggcgcttc gtgggatatc ggcacattcg
     2821 ccgatcgacc tgagccggaa tggttcgatg cgttgtcgac ttcgccgcat gggtgggaaa
     2881 tgatcgacgt gcgtggaaaa agcttgtccc cggggggtca tcgtccgaag ggccatgccg
     2941 ctcaagaccg agcgacttgc cgcctgagag agcgacaatc gagtcattct gccgatgagc
     3001 caaacagcgg ccgactcgca gatcgctcgc tttgccggga tcgaccatgc atccattcat
     3061 ggatatcacg gcgaagaaaa cgccagtgtt ttccaatctt tgatgccggc agttcaccaa
     3121 ctcgggcgag tttatagacg gtggacttcg gtacccgaag aaaccgagcc acttccatca
     3181 cggttagaat ttccccttcc cgtgctgtgg cctccacggc ctggatcgct gttgctgtag
     3241 atacttcatc attcatgaca actgaggtat cggcgtggcg gttcaaaaac ttgagcgtcc
     3301 aataggaacg aatttgggac ggcggcctta cttcttccat tccactcgga atgctatcca
     3361 ttcacgtttc tccgcgactg accgacaaag gatatgccaa tgggcccaaa tcaataggaa
     3421 ctgtactcaa tggcacatgt gatctcagtt gcgtcaggaa aaggtggggt cggcaaaagc
     3481 gtggtttctg tcaacctggc tcttgtcttg gcacgtaagg agaagcaagt agtgctcgca
     3541 gacctcgatg tcgggggcgc cgacgcgcat gtgctggtcg gactcctcaa tccgcccctt
     3601 accctgaccg acttcctcaa ccatcgaatc gagcgcctgg atgatctggc tcaaggtctc
     3661 tccattcacc cgaacttgcg gattattccc ggcaccggcg acacattggc gacggccaac
     3721 atggcctatt cacagaaaaa gcgcttgatc cggaactttc aggatatccg tgcggacgtg
     3781 atcatcgtcg atatcggagc aggaacaggc tatcatgcgc tcgatttttt tctgatggcc
     3841 gaccatcacc ttgccgtcgc aacccctgac ccgacctctg tcctggatct ctaccgattc
     3901 atcaagctgg cggcgattcg tcgtgtcttg tcgatgttcg tcatgcggga tgtcatggcg
     3961 gagaggcttg cgaaccgtga ctacagcagt gtggaagaag tattggacgt ggcaggcaag
     4021 accgacgaat ccggtcggtc gattgccgag tctaccctca aagcttttca accggctctc
     4081 atccttaatc gcgtttcagg gcgggctcgc gtcaatgtac cgcagctcaa gaagttactc
     4141 aaggagtatg tcggaggtga cctaacctta ttgggtgaga ttcccgaaga ccacgcgatg
     4201 gaacaggcgg tacgcgcata cgtcccggtg gttcagcata ctccatcgtc tcctgctgcg
     4261 gtcgcgctcg cgaaaaccgc cgatgccctg gcacggctgc ttcacgacgc accgccgcaa
     4321 gcggcatgat tcggttgagc ggtccgaata ttccccatgc cgtttctcgt caatctccac
     4381 tggttactca taagagagcc gttcttgtaa tccgcgtgaa ccggtcgctt gtgcaccgat
     4441 agactccagt gatcatcgaa gcttttgtac gatcactgca taccatccga ggccgtcgta
     4501 ttcctggtag ccgagacttt tcgcatacgc gatcacttcg tccttgtcgt tcacgtaatg
     4561 gcctcgttgg cgtccttgat gctgaagcgt gaacggcaca aggagtcctt gatgatcgga
     4621 tgccgcgata actcgcagcc tgttatccag cagcatcacg cgggtgcgtc tccatgacgc
     4681 ttcggatatt gccggctctt tctgaacaat agttccggat tgctcctccc aatcgaatac
     4741 cacccctaaa acgccgatga tccttccgtt tgtgtttcct cccttccgaa cggctgcggc
     4801 atacacggca accaatttgt cgccatggag gggatcataa caaatatcgt cgactacata
     4861 ctggtcgccg ttcgtcgtcg ccatggcttt ctggaaccag gacactctca ttgtatcggc
     4921 tccgattatc ctaggaaact tttcagggtg tgagcaggcc atgatcttgc cgtcgatccc
     4981 aaccaataca aggttgagat acacagaata atatcgattg atcagaccga gtcgtccgac
     5041 tgcatgctgg agagcagctc gatctaacga ctccaaaccc ttgaccaacg cttcatccat
     5101 cgcccaccaa cgaacgtcag cggtacgttc gtacagattg cgtacgatca actgcaccag
     5161 ggtctgcgcc atctccgaga gacgatcatg ctctttgtcc ataaaccgtc gattcaattc
     5221 gctggtttct tcgcgtatcg aactcagggc tttggaggcg tcggccgatt gcgcagccag
     5281 gtgtttcacc tctgtcgcga ccacggcgaa cccttttccg gcatcaccgg ctctcgccgc
     5341 ttctatggtc gcgtttaacg aaagaatgtt gatctcgctc gttattcttg cattcacaag
     5401 ccggtgatcg tcgatccgct tttcgatgtt accgatgatc tctttgatat tgagctggtt
     5461 cattcacgtc ccctggattt tccgcaactt tgacggcaac gccagaattc ttcggtgaca
     5521 tccgtatggc cgtgaaccat tctctcccct aaccgggatg attgcggcgt gcgcgcaagc
     5581 gttcggcctg tgcgtgaaga atgtggcgga tgaggtggtc cagatcatcg gaactgatct
     5641 cgagaaactc cgtggcaaga acccagcctt gtctctgaga atccggcgtc gatcggataa
     5701 cttgggtaac ggcgtggatg ggagtaaacg gaggcagg
//
LOCUS       DATNDD010000418         3967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3020938, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000418 DATNDD010000000
VERSION     DATNDD010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020938"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VN647_10150"
     CDS             complement(<1..640)
                     /locus_tag="VN647_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97434.1"
                     /translation="MARKKERQVSILGSSYFQPIANLVERWKAFTRHRPNKVQSGFYE
                     SGYASSVILLLVAMFESYVVRLRFVQGPTVPATARTALDVVFSVFPQLRHRKALIDVY
                     VLRDSIFHNHLWEIEYSYGGSPTMVMHLASKHPAFGDKKYAARVNPTTRRTRALDLNI
                     VPTRVDRRDVLKVFSTIWKTLLLFEAQNKSQCYVSHETVRFAAKTMPFSDVID"
     gene            complement(855..1091)
                     /locus_tag="VN647_10155"
     CDS             complement(855..1091)
                     /locus_tag="VN647_10155"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HWG97435.1"
                     /translation="MAEVIDILSLVPQSLNLRSNHLWLDYDDEADVLYISLRKPQWAT
                     NSEMAGRFIYHYDGNELVGVTVLHAKGQRGTDAA"
     gene            complement(1076..1402)
                     /locus_tag="VN647_10160"
     CDS             complement(1076..1402)
                     /locus_tag="VN647_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97436.1"
                     /translation="MEPLATLISKRGVRVRLTHERWSHIVESHDYLAGHLDLFVETVS
                     DPDCVVTGVKDELLAVKRYDETAIGAKHVVVAYKEQGQDRFIITAFMTSKIDKLLKRG
                     IQWQKS"
     gene            complement(1785..2090)
                     /locus_tag="VN647_10165"
     CDS             complement(1785..2090)
                     /locus_tag="VN647_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWG97437.1"
                     /translation="MAILVDTSVWIDHLRTNSPALRRLLDSDLVVCHPLVIGEIACGH
                     MKHRSEVIESLAVLPTTPIIEYQEVLTFIETHKLFGQGLGWIDVHLLASTMLQQVTL"
     gene            complement(2093..2284)
                     /locus_tag="VN647_10170"
     CDS             complement(2093..2284)
                     /locus_tag="VN647_10170"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWG97438.1"
                     /translation="MRTTLNIDDHLIKRAVQLTGVQEKTQLVRLGLEALISREAAKRL
                     ARLGGTEPHLKDIRRRRSA"
     gene            complement(2433..3566)
                     /locus_tag="VN647_10175"
     CDS             complement(2433..3566)
                     /locus_tag="VN647_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG97439.1"
                     /translation="MLRRLRGMLVKEFIQVFRDTRTRTLLFAPPIVQMLIFGYAATLE
                     VRHIPTAMLDYDNSQVSRELLSRFTASLYFDVRAYLNDRREIGDLIDRGEVAVALQIN
                     AGFAQDLRKGQTARLQVIVDSSNSNTALVAVGYINQIAARFARDFQRTQLLRTSPFAP
                     SQMPSIVLERRPLFNVNFDSQWFFVPGVIGNLILVMVVSLTAFAIVREREIGTLEQIM
                     VTPIGRLEFILGKTVPFFLIGLFDTALVSLVGTLWFGVPFRGNIGVLAVGSILFILCM
                     LGIGLFISTISRTQQQAMVSGFFFNMPAITFSGFGTPIGSMPEWLQTLTYLNPLRHFL
                     EVLRGVYLKGVGLDVLWPQMVAMGLLGSIMLAISVARFQKSLD"
     gene            complement(3576..>3967)
                     /locus_tag="VN647_10180"
     CDS             complement(3576..>3967)
                     /locus_tag="VN647_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG97440.1"
                     /translation="AILWFEVPFRGQWTVLFLSCTLFLVVVLSLGYVISVVAKSQLAA
                     SQAALIATFLPAFLLSGFLYPIDQMPTVIQVITHLIPARYFITIIRDVFLKGTSLPFL
                     MDDLLALALFATILTILATRAFHKKLA"
BASE COUNT          992 a         1014 c         1103 g          858 t
ORIGIN      
        1 ggtcaatcac gtcggaaaat ggcatggtct tcgccgcgaa ccgaacagtc tcatgagaga
       61 cataacactg ggatttattc tgagcttcaa agaggagcaa tgttttccag attgtagaga
      121 agacctttag cacgtcgcgt ctatccacac gcgttgggac aatattgagg tccaatgccc
      181 gagtgcgacg tgttgtagga tttacacgag cggcgtattt cttgtcacca aatgcaggat
      241 gctttgatgc cagatgcata accatagtcg gagagccacc gtaagaatac tcgatttccc
      301 aaaggtgatt atggaagatt gaatcgcgga gtacatatac atcgatcagc gccttccgat
      361 gccgaagttg tggaaatacc gaaaatacca catcgagtgc tgtacgtgcg gttgcaggca
      421 ccgtcggtcc ttggacaaat cgtaagcgca caacgtacga ctcaaacatc gcgacaagaa
      481 gtagtattac actcgacgca tagcccgact cgtaaaatcc tgactgaacc ttgtttggac
      541 ggtggcgcgt aaacgccttc caccgttcaa cgagattggc aatgggctga aagtatgaag
      601 agccaagaat gctaacctgc ctttccttct ttctggccat tgccttatgt cactaacaat
      661 gtttagaggg cacaactaag gggtccggct ccataaattc tctttgagcg gcgcgctgcg
      721 gtttggctcg tccgctctca aaggcgtggt tatacggctt ctacttcgac gtgatcgctc
      781 gtggaggcag gagccggaat cggttgaccc tgttctttca atccctctaa gtaaagcaca
      841 atagagcctc gccactaggc ggcgtctgtt ccgcgctggc ctttggcatg caggacagtg
      901 acgccgacca gttcgtttcc gtcgtaatga tagatgaagc gacctgccat ttcgctgttg
      961 gtcgcccatt gcggttttct caggctgata tacagcacat ccgcttcgtc gtcgtaatcg
     1021 agccacagat ggttggaacg caggttcaat gattgcggga ccaagctcag gatgtctatg
     1081 acttctgcca ttggatgcct ctcttgagca gcttgtcgat ttttgaggtc atgaaggctg
     1141 tgataatgaa ccgatcctgc ccctgttcct tgtaggcaac cacgacatgc ttagcaccga
     1201 tcgcagtctc atcataacgc ttgaccgcca acagttcgtc tttgacgccg gtcacgacac
     1261 aatccggatc acttaccgtc tcgacaaaaa gatcgagatg cccggccaga taatcgtgtg
     1321 actcaacaat gtggctccac cgttcatgcg tgaggcgcac ccgaacgcca cgtttggaaa
     1381 tcagtgtggc gaggggttcc acgctcgctg atcactccag acttaagatt aagattctgt
     1441 accggcgcga atctcgacca catgattgtc agtggctggt cgtatcgagg gctttagtat
     1501 tgcccaggct gactgctatt gcaagttttg gaggctgaca gttcatcaca acggcatggt
     1561 ttattaagcg agccaacggg gtccagcctc gaaatttttt accgacttct caggctgcat
     1621 cggtttcgat cttgccggga agcttgctga ggatcagttt ttgtagttcg ggagacattt
     1681 aagggggcgg ataccttttt aataccagga gtggtcgctc agtcggacga ttcaaaatga
     1741 cagcgcagtc ttctggcggc gtttcgcaaa ggctgatcga gggtctagag tgtcacttgc
     1801 tggagcattg tcgaggcgag gagatgtacg tcgatccaac ctaagccctg tccaaagagt
     1861 ttatgggttt cgataaacgt caggacttcc tgatattcaa tgatgggcgt ggtaggcaac
     1921 acggccagag actcgatcac ctcagaccga tgtttcatgt ggccgcaggc gatttctcca
     1981 atgacgagcg ggtgacatac aaccaggtcg ctgtccagca gccgtcggag agcgggagaa
     2041 ttggtacgaa ggtggtcgat ccacacggaa gtgtcgacca gtatggccac gactaggcgg
     2101 accgacgtcg gcggatgtct ttcagatgtg gttcagtgcc gcccaatcga gcgagccgtt
     2161 tagcagcttc gcgtgagatg agggcctcga gccccaagcg tacgagctgc gtcttttcct
     2221 gcacgccggt gagttgcact gcgcgcttga tgagatggtc gtcaatattg agtgtggtac
     2281 gcatgtgcat gaatatgcat ggtcgaggca tatactgtca agcattgcat tgagtcacca
     2341 cggccagaaa aaaatagagg gcgtggatca tcctcgtgct aaagatccac ttcggttagg
     2401 cgtcaagcca tcagccgcag acaagaggaa gctcaatcca gtgacttctg aaagcgcgcc
     2461 acgctgatcg ccaacatgat tgagcctaaa agacccatgg cgaccatctg cggccacagc
     2521 acatccagcc ctacgccttt taggtagacg ccgcggagga cttccagaaa atgccgcaac
     2581 gggttgagat aagtgagagt ttgcagccac tccggcatgc tgccgatggg ggtgccgaat
     2641 ccggagaagg tgatggccgg catgttgaag aaaaaccccg acaccatcgc ctgctgctga
     2701 gtcctcgaaa tagttgagat aaataacccg atgcccagca tgcagaggat gaacaggatg
     2761 ctgccgactg cgaggacgcc gatgtttccg cgaaacggca cgccgaacca gagggtgccc
     2821 accaacgaaa caagggcagt gtcgaacagt ccgatcagaa agaaaggcac cgtcttgccg
     2881 aggatgaact ccagtcggcc gatcggtgtg accataatct gttcgagcgt gccgatttcc
     2941 cgctcgcgga ctatggcaaa cgccgtcagg ctcacgacca tgaccaaaat caggttgccg
     3001 atcacgccgg gcacgaaaaa ccattgactg tcgaaattga cgttgaacaa gggtcgacgc
     3061 tcgagaacaa tcgaaggcat ctgcgacgga gcgaaagggg atgtccggag aagctgcgtt
     3121 cgttgaaagt cccgggcgaa tcgcgcagcg atctgattga tataaccgac cgcaaccaag
     3181 gcggtattgg aattactgct gtccacgatc acttgcaacc gagccgtttg acctttccga
     3241 agatcctgag caaaacccgc gttgatctga agcgccaccg cgacctctcc gcgatcgatc
     3301 aggtcgccga tctcccgacg atcgttcagg tatgcgcgga catcgaagta aaggctggcg
     3361 gtaaagcgcg ataggagctc tcggcttact tggctattgt cgtaatccag catcgccgtg
     3421 gggatatggc gaacttccaa ggtcgcggca tagccgaaga tgagcatttg aacgatggga
     3481 ggggcaaaca aaagcgtacg agtgcgtgtg tcccgaaaga cttggatgaa ttccttgacc
     3541 agcatcccgc gaagacgtcg cagcatgaag aatgcctatg ccagcttttt gtgaaacgcc
     3601 cgcgtggcca ggatcgtcag gattgtggcg aagagagcca acgcaagcag atcgtccatc
     3661 aaaaaaggaa gcgaggttcc tttcagaaag acatcacgga tgatcgttat gaaatagcga
     3721 gccggaataa gatgggtaat gacttggatc acggtcggca tctggtcgat ggggtagagg
     3781 aaccccgaga gcagaaaggc cggaagaaac gtggcgatca acgccgcttg gctggcggcg
     3841 agttgagatt tggccaccac cgaaatgaca taccccagcg acagaaccac gaccagaaac
     3901 aaggtgcaac tgaggaacaa caccgtccat tgccctcgaa agggcacctc gaaccacaag
     3961 attgcca
//
LOCUS       DATNDD010000419         3191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1892295, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000419 DATNDD010000000
VERSION     DATNDD010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892295"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            434..>3191
                     /locus_tag="VN647_10185"
     CDS             434..>3191
                     /locus_tag="VN647_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWG97441.1"
                     /translation="MGDRVCTWLQSLGLDRYRELFEQNAITWDVLPELNDGDLASMGI
                     VLGHRKQLLRAMAQLSQRGEGETATTLAIPTAKDTTPSSSSRDQAERRQLTVMFCDLV
                     GSTALAQRLDPEDAQAAIRRFLDTCSEAIGRFNGYIAKYMGDGLLVYFGYPQAHEHDA
                     ERAVHAGLAVLQVVTTLPRDSVPHQEFAIAARIGIATGHVIVGELMGQDTAKERSVFG
                     ETPNLASRLQGLAAPNQLLVDSTTKRLVGDEFELADQGMVSLKGFAIPAQVWQVLGSK
                     PSASRFESYRSRRLTNFIGREHETALVLGRWREAVDGEGQVVLLCGEAGIGKSRLVRY
                     LRDRLDKDHYESIQLQCSPHHTNTALYPVITNLRQAAGLTDEDNVSTQLHKLDTLMAD
                     SGPNDRVMVALLADLLSIRGDEQDQLLNVSPDKRKDMTLEALVQYLHRLADRSPILLI
                     VEDAHWLDPTTLDLMTRIIDRIRQMRVLLLITLRPDFRPVWAEYSHVTFLTLSRLPRR
                     QSAELIATMTRGKVLPQEVQQAILAKTDGVPLYIEELTENLLETGMLAEGTDSFTLKA
                     PLREMVIPDSLQALLMERVDRLGPVKEIVQIGAAIGREFTYELLRAAVHVTDAQLNSA
                     LDLFVASGLILQEGERSLSRYRFKHMLVQEAAYNTLPKKTRRLLHARIAKTLEEKFAD
                     RVQLEPEFLAYHFEQAGLTRPAITYWRLAARRDVDRSANVEALNHFHSALTLLKDLPA
                     DTERDALELELLIARGAPMVTVKGYASPEIEHNYLRARELSRENSYSEHHFLAVWGLW
                     VFHLVRGPLATACDLAEHLLFLAKHEQNPDLLIRAHESIGSTYSFLGRFDEAKAHLLA
                     AKSLYDPARHRPQVFPYAQDPGITARIMLARTLWILGEVDQVEALLQEAIGMARELEH
                     PFTLA"
BASE COUNT          685 a          865 c          928 g          713 t
ORIGIN      
        1 gggacggcgt ctggcgctgg ggttgactgc tgcggcaagt attcttgcct ctgggcttac
       61 cgctacctcg gcaaccgcgg attggatccc actcacgttc gggatcgtcg gtgcactgtt
      121 gatcggagga ctgtttttcg atctgatgcg agggcgttaa cgacgagttc ttctcgtcgg
      181 catcaaggtc cggttctccc gcgcgtgatc gaacgtagaa tcactctctc gagtaggtat
      241 gcgatgacgg acattccgat ggagatgaaa cggtactcga tttttcgtac gaggggctga
      301 tgaagcatct tcgtcgatcg gttaatcata tggtcattat caacgacggc atcgcggtct
      361 gcaagagagg cggaatcctt ctccagcgcc tcatcgatct atggtggaac gatcaactga
      421 gacggtgttg atcatgggtg atcgcgtatg cacatggttg caaagtcttg gccttgatcg
      481 ctatcgagag ctgtttgaac agaacgccat cacatgggac gtcttgcctg agctgaatga
      541 cggcgatttg gcgtcgatgg gcattgtcct tggccatcgg aaacaactct tgcgcgccat
      601 ggcgcaattg tctcaacgcg gtgaaggcga gactgcgacc actctggcca ttcccaccgc
      661 gaaagacacc acgccgtcct cttccagtcg agatcaagca gaacgacggc agttgacggt
      721 catgttttgc gatctggtgg gttcgacggc gctggctcag cggttggacc ctgaagatgc
      781 ccaagcggct atccgacgtt ttctcgatac ctgtagtgaa gcgatcggcc gattcaacgg
      841 ctacattgcc aaatacatgg gtgacggact tctggtgtac tttggatacc ctcaagccca
      901 tgagcacgat gcggagcgag cggtgcatgc aggattggct gtattacaag tcgtgacgac
      961 cctgccacgc gattctgttc cccatcagga atttgcgatc gcggcaagga tcgggatcgc
     1021 gaccggccac gtgatcgtgg gcgaacttat gggacaagat accgccaagg agcggtcggt
     1081 gttcggtgaa actccgaatc tggcttcccg cctccaggga ttggcggcgc cgaatcaatt
     1141 gctcgtcgat tccacgacaa agcgtcttgt cggagacgag ttcgaattag cggatcaggg
     1201 aatggtttct ctcaaggggt tcgcgatacc ggcccaggtc tggcaagttc tgggcagcaa
     1261 gccttcggcc agccgattcg agtcttatcg gtcccgacga ttgaccaatt tcataggccg
     1321 agagcacgaa acggcgcttg ttttaggacg ctggcgcgaa gcggtggacg gtgaaggaca
     1381 ggttgttctt ctctgcggcg aggccggcat cggcaagtcg cggctcgtcc gatatctacg
     1441 tgaccgcctt gacaaggacc attacgagtc gatccaattg cagtgctccc cacaccatac
     1501 caatacggcg ctctatccgg tgattaccaa tctacggcag gcagcggggc tgaccgatga
     1561 agacaacgta tccacgcaac tgcataagct tgacaccctg atggccgaca gcggacccaa
     1621 cgaccgtgtc atggttgcct tgctcgcgga tctcctttcg atccgaggag acgaacagga
     1681 ccagttgttg aatgtgtcgc ccgacaaacg caaggacatg accctggaag cgctcgtgca
     1741 gtatctgcac aggctggcgg atcgctcccc tatattgctt atcgtagagg atgctcattg
     1801 gcttgatccg accacgctgg acctcatgac gcgaatcatc gaccggatca ggcagatgcg
     1861 cgtgttgttg ctgatcaccc ttcggccgga cttcaggccg gtttgggcgg aatacagtca
     1921 tgtcacgttt ctgacgttga gtcggcttcc tcggcggcag agcgccgagt tgatcgcaac
     1981 gatgacaagg ggaaaagtgc ttccccagga ggtccagcag gcgattctcg cgaagaccga
     2041 cggcgtgccg ctgtatatcg aagagttgac ggaaaatcta ctggagaccg gaatgctggc
     2101 tgaagggaca gactcattta ccctcaaggc tccgttaagg gaaatggtca ttccagacag
     2161 cctgcaagcg ttgctcatgg aacgggtgga ccgtttgggg ccggtcaagg aaatcgttca
     2221 gatcggagcg gcaatcggac gtgaattcac ctatgagctg ctgcgggccg ccgtccatgt
     2281 gacggatgct caactcaaca gcgccctcga tttgttcgtc gcttcagggc tcattctaca
     2341 agagggtgaa cgatcgcttt cgcgatatcg ttttaaacat atgcttgtcc aagaagctgc
     2401 ctacaacacg ctccccaaaa aaactcgccg actcctccat gcccgcattg ctaaaactct
     2461 ggaagagaag ttcgccgatc gcgtgcagtt ggaaccggaa ttcctggcct accactttga
     2521 acaggcaggt ttgactcgtc cggccatcac gtattggcgt cttgcagccc gacgagacgt
     2581 ggatcggtca gccaatgtcg aagcgctgaa ccatttccat agcgcattga cgttgttgaa
     2641 agatttacct gcggacacgg agcgcgatgc attggagctg gagctcctca tcgcgcgcgg
     2701 agcccctatg gtgactgtga aaggatatgc ctcgccggag atcgagcaca attatctcag
     2761 ggcgagggaa ctctctcggg agaacagcta ctccgagcat cactttctcg cagtgtgggg
     2821 gctgtgggtt ttccatctgg tcagggggcc tctcgcaacg gcgtgcgacc tggcggaaca
     2881 tctcctgttc ctggcgaaac acgagcaaaa cccggacctg ctgattcgag ctcacgagag
     2941 tatcggctcg acctattcct tccttggccg gtttgatgaa gccaaagccc atttgttggc
     3001 cgccaagtct ctgtatgatc ccgctcgaca tcgcccacag gttttcccct atgctcagga
     3061 ccccggcatc accgcgagaa ttatgctggc cagaacatta tggatattgg gtgaggttga
     3121 tcaggtcgaa gcgctgttgc aggaagccat tggcatggca cgagagttgg aacacccctt
     3181 cacgctggca t
//
LOCUS       DATNDD010000420         6365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3072076, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000420 DATNDD010000000
VERSION     DATNDD010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3072076"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VN647_10190"
     CDS             <1..805
                     /locus_tag="VN647_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97442.1"
                     /translation="FNLLLELSEALYENERLLTESRTRVEQLIQILGPAIERPSIAGE
                     VEDYIIFSIESCVPERPPLWTSSEADLAQILRAERTPFSEQEVADAVSCRISFGQDDA
                     AVIDWHSAVLFGKEMDDVRAVLEFANVELLEMRILDEQLDRALDEGYEALSRKPRLLS
                     LPGSHEKDMTHIAQLQVDSALLFERVTNTLKLLGDQYLARVYRLVSQRFHLEAWDASI
                     LRKLQTLESIYGKMSDRAGTRRMEVLEWIIIILITLSIAISFLQMRADH"
     gene            1004..1519
                     /locus_tag="VN647_10195"
     CDS             1004..1519
                     /locus_tag="VN647_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97443.1"
                     /translation="MAIATSQLSGRREFPLGAQEVVISSGSSGINGMVVTSRRLLGFS
                     SHALTWSKKDLDVNEKVLEREILPTFSLIRTDRHLYGFRGVNGLWLEEALGVREQVSC
                     FHSNDYGAVFITNERLIGFTPLIGGFASKPLDVHERVVGVDNESGLIVVSTTRRTLVF
                     GSRLSGWEEFE"
     gene            2239..2622
                     /locus_tag="VN647_10200"
     CDS             2239..2622
                     /locus_tag="VN647_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG97444.1"
                     /translation="MVTVGNLMQKEPVTVDAGTSVIEAAKLMKACNVESVLVTHQARI
                     IGILTESDVVKKFVGAEKVSYFVPVEDIMSSPLPGIEERRPLTEAADLMDKHRTLHLG
                     VTKGGTFIGLVSVRDFLRPVSIDDF"
     gene            2767..4251
                     /locus_tag="VN647_10205"
     CDS             2767..4251
                     /locus_tag="VN647_10205"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HWG97445.1"
                     /translation="MTRLDTHLAPHSRFSAISSQPERELKLAVAPNFHLPRLAGTPLP
                     RRQLISTYYDTAAYDLAHARITLRHRIERGKKSWQLKIPLGIDRQEIEVTDEDAGPPA
                     SLRRLLTLHLGHRKLLPVVTLCVWRRGFLVRHGRVPIAEIALDSVSVDKNGRTTRCFR
                     ELEIEQRQGDEASLRSLEQRMREAGASDHDGRPKLFRALSLSAPSQPMQPEAKAPTVE
                     YIKWALAQHVGWLLAHDPGTRLGTDCESLHQMRVATRRLRAVLRTARPILLPAWLASL
                     QQELKWLSELLGPARDLDVQIAYFTEKSSALDARDRKLLTAFISHLRTQREAVQQMVL
                     SELTSARYFELIRRLQQAAQAPSVVESSFTVRRLAKRAFKKLRKAINRLAPSPSDTQL
                     HKVRIKAKRARYAAELARRSVGKPAGRFIKSARVVQDLLGIHQDAIQAERHIRQFLKY
                     STSLRAGFVAGRMVERQRQRRHQVRKDMKPLFKALLKRGKQAWG"
     gene            complement(4954..5601)
                     /locus_tag="VN647_10210"
     CDS             complement(4954..5601)
                     /locus_tag="VN647_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97446.1"
                     /translation="MTLLKQVTSLLGLIGVLTAGTSGVWAIDVNLSSDEAHKALEIGR
                     IPMEKTNSPEDVKKVLQQASLATRVGADPEKDPCGASAVLRTKRYRLEAFGRQEAAES
                     KKRKTDVRMPEEFIQKVMDMPNMELEVQLCGDDEYFAEGALIELQQGSKRIKPIDIGK
                     AERGRKNESNGPAFRSRFTALFAYEQFDPTASSVFVVNLQDGTEIRISADLSKVK"
     gene            complement(5698..6330)
                     /locus_tag="VN647_10215"
     CDS             complement(5698..6330)
                     /locus_tag="VN647_10215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWG97447.1"
                     /translation="MQLVLASNSPRRRELLALLGLSFEVCPSEFHEQPTVGLSPVEQV
                     RRFAREKATFVALVRPQALVLGSDTVIDLEGRLLGKPVDLADARAMLARLAGRSHHVH
                     TAIALCDRVRHIESVEVATAEVRMKADRDQAYERYLASEESFGKAGAYAIQGLGGDLV
                     ERIIGDYTTVVGLPLKLVAQLLRSADYPLLVNVEDLYRRKPYTNWNRFTT"
BASE COUNT         1401 a         1838 c         1755 g         1371 t
ORIGIN      
        1 attcaacctg ttgctggaac tcagcgaggc actctacgaa aatgaacgac tactgacaga
       61 gtcgcgcact cgcgtcgaac agttgatcca aatactgggt cctgcaatcg aacggccgtc
      121 cattgcagga gaggtggagg attacattat cttctcgatt gagtcctgtg tgccggagag
      181 gcctccgttg tggacaagca gcgaggccga cttggctcag attctgcgtg ctgaacggac
      241 tcccttctct gagcaagaag tggccgacgc cgtgtcatgc cggatctcgt tcgggcaaga
      301 cgacgcagcc gtcatcgact ggcattcggc cgtcctgttc gggaaggaga tggacgacgt
      361 ccgtgcggtc ctggaattcg ccaacgtcga gttgctggag atgcgaatac tcgacgaaca
      421 gctcgatcgc gcactggacg agggttacga agccttgtct cgaaaaccgc gcctcctgtc
      481 gctgccgggc tctcatgaaa aagacatgac gcacattgca cagctgcagg ttgacagcgc
      541 cttgcttttc gagcgcgtca ccaacacctt gaagctgctg ggcgatcagt atcttgcgcg
      601 tgtctatcgg ctggtctcgc agcgctttca tttggaggcg tgggatgcca gcatccttcg
      661 caagttgcag acgctggaaa gcatctacgg caagatgtcc gaccgggccg ggaccagacg
      721 gatggaggtg ctggagtgga ttatcattat tttaatcacg ctttccatcg ccatctcgtt
      781 tctccagatg agggcagacc actaagtgtt tcttgcgctc atttagtgga gaagactcag
      841 gaaccgatac gcattatctc tgaggaatat gaacccgact gagccctttc actctttcgc
      901 ccggttcttc tgcacaacct atcttcctat ggctttgata gtttcaccag tcgtcgctac
      961 ggcaaaccct caagctgagg tcgctttacg gttcgaatcg ggtatggcca tcgcgaccag
     1021 tcaactgagc ggacgaaggg aattcccgtt gggtgcgcag gaagtcgtaa tcagttccgg
     1081 ctcaagcggg atcaacggca tggtggtgac atcacgtcgg ctcttagggt tttcaagcca
     1141 tgcgttgacc tggagcaaaa aagacctgga cgtgaatgaa aaggttctcg aacgggaaat
     1201 cctccccacc ttcagcctca tcaggacgga tcggcatctg tatggatttc gcggcgtcaa
     1261 cggtctgtgg ctcgaagaag cgttaggtgt gcgagaacag gtgagctgtt tccacagcaa
     1321 cgactatgga gcggtcttca tcaccaatga gcgattgatc ggattcacgc cactcattgg
     1381 cgggttcgca tcgaagccct tagatgtaca cgagcgagtc gtgggcgtgg ataatgaaag
     1441 cggcctcatc gtcgtttcca cgaccagacg cacgctggtc ttcggcagcc ggttgagtgg
     1501 atgggaggaa ttcgagtgat cgatctccag tgcagatggt gttagccaca tcctaacctc
     1561 gcgaccagca cggatatcgg cgaaagcgct gtcatagggc aagtctatga cgagataatg
     1621 ccacggcaga acagccagtc cggcggcatt cctgacctca tgtccatcgc atcccctttc
     1681 ttcacacaga attggcacga tcgtaacgag ggtgaaacaa tccctcgata ggctgacatc
     1741 gtgttcatca gggacagaac cacgattctc acaacatgga agggggtgag aaactatgat
     1801 gacgtcatcg gtcgtgatcg taggaacgct cgtattcgtg accgttttgg gtgcgctggt
     1861 taaagtgctt tccctcatgg gtcgcgagta aaccaacaca accacaacct gaaaggaggt
     1921 gacaagcgat gacgatgacg gtgtggatca tcgaagcgtt cctgctcagc gccattatag
     1981 gactggcgta tacggtacta tctttgggcc gcgagtaagg aaacgcctca tgagcaaagg
     2041 agacctgact gtgaatggag attgtcagac gagagccgca cggggccctg ttcagcggct
     2101 ctcgtcgaaa aggaaaaatt gaattccccc ctcttcattc ttccacattt tactggcccg
     2161 taatatctac ctaaccccgt cgtaacagca cattggtagc ctggagccaa tacaaacata
     2221 ataaaaagga ggatcagtat ggttacagtt ggcaatttaa tgcagaaaga accagtgacg
     2281 gtggacgcag gcacctcggt catcgaggcg gcgaaattga tgaaagcctg caacgtcgaa
     2341 agtgtgttgg tgacccatca ggcgaggatc atcggcatcc tgacggaatc ggacgtcgtc
     2401 aaaaagtttg tgggagccga gaaggtgtcc tacttcgtgc cggtggagga catcatgagc
     2461 agtccgctgc ccggcatcga agagcgccgg ccgttgacgg aagccgccga cctcatggac
     2521 aagcaccgca cgctccacct cggcgtgacc aaaggaggga cattcatcgg cctggtgtcg
     2581 gtgcgggact ttctccgacc ggtgtccatc gacgactttt aacgacccta cctttcttga
     2641 ccccgctgcc tagttggcta ggcagcctcg gcgaccttcc ctggtattcc ccgacgcccc
     2701 tgagttgcat ttcctctctc ctgcgttgac actgaaggga gcccggctct gcccgagggt
     2761 tccacgatga ctcggttgga cacacatctc gctcctcata gccgcttctc cgcaatctca
     2821 tcgcaaccag aacgcgaact gaagctagcc gttgctccaa atttccacct gcctcggctt
     2881 gccggcacgc cgcttccccg gcggcaactg atctccactt attacgacac tgccgcctac
     2941 gacttggctc atgcgaggat tacccttcgt catcgcatcg aacgggggaa gaagtcctgg
     3001 cagctgaaaa taccgttggg cattgatcgg caggagatag aagtgaccga cgaggatgcc
     3061 ggcccgccgg cctcgctgcg tcgtctactg actctccatc tgggacatcg gaaactgttg
     3121 ccggtcgtga cgctgtgcgt ctggcgaaga ggtttcctgg tgcgtcacgg tcgagtaccc
     3181 atcgctgaga tcgccctcga ctcggtgtca gtcgacaaga acggccgtac cacccggtgc
     3241 tttcgcgaac tcgaaatcga acaacggcag ggcgacgagg cctcactccg ttccctcgaa
     3301 cagcggatgc gagaagcggg tgcatccgat cacgacggac gacccaagtt attccgtgcc
     3361 ctctcgctct cagccccttc tcaacctatg cagcctgagg cgaaggcgcc gacggtcgaa
     3421 tatataaagt gggccctcgc tcagcacgtc gggtggctcc tcgcccatga tcccggcacg
     3481 aggctcggaa ccgattgtga gagcctgcat caaatgcgtg tcgcgacgag acgcctgcgc
     3541 gccgtgctcc gcaccgcgcg accgattctc ctccccgcgt ggctcgcgtc actgcaacag
     3601 gagctcaagt ggctcagcga gctgctggga ccggcccgcg atctcgacgt ccagatcgct
     3661 tatttcacgg agaaatcttc cgcattggac gcacgagatc gcaagctgtt gacagcattc
     3721 atctcccatc tgcgcactca acgagaggcc gttcagcaga tggtgctcag cgaactcacg
     3781 agtgctcgat acttcgaact catccggcgg cttcaacaag cggcgcaggc cccctcggtc
     3841 gtggagtcct ccttcaccgt ccgccggctt gccaaacgag cattcaagaa actgcgcaag
     3901 gcgatcaacc ggctcgcccc ttctccatcg gacactcaat tgcacaaggt ccgcatcaag
     3961 gccaaacgcg cccgctatgc ggcggaactc gctcgtcgct cggtggggaa acccgccggc
     4021 cggtttatca agtccgcgcg tgtcgtccag gatctactgg gcattcatca ggatgcgatc
     4081 caagcggaac gtcacatcag acagttcctc aaatactcga cgagcctccg agctggattt
     4141 gtcgccggtc gcatggtgga acgccaacgc caacgacgcc accaagtccg caaagacatg
     4201 aagccgctgt tcaaggcttt gctgaaacgg gggaagcagg cgtggggatg aaaatggtca
     4261 atgcccccat cacaattatt ggtgaacggt ccaggacaag cttacgatcg acaccgaacg
     4321 gccgtcttct aaggctcccc acgtttctag agaggggctc atttccccaa gacccgcagt
     4381 tgcatcgact aaagccatta gcccagccgc ccctggcccg gcttcatcac gccttttgat
     4441 tcgacacgcg ctgccccggc ttttttgagc ggaaagtggg gaagggcttt tccacacagc
     4501 aatagcacta caacttcacc aaggtatcgg accatcgttc cagagcggtc acgaacaggt
     4561 aaaacctgtg ttaactgcgt tgtccctctg cggtaggctt gtgcctgcta tgtggaggag
     4621 tggttatgca ctgcattctg gttcaccacg gcatcgcggt agagtctatg atacagccag
     4681 gctgctacgc acggtcatct gttcgactct caaggtcgag acacgggaag aatcggcggt
     4741 cggagcgaag cctgagcagc tggttggatt gctccgtaca ctcccgttgg atgcggtggt
     4801 ggtgtgcgtc aggcatgaac ctcagcttcc tgtttagctg gaacggaaaa gaaaggatgc
     4861 catgtcagca ctcgaagcgg ttcgtccttg gaaccctgcg tgcgaagata gataaggaag
     4921 tcgcttaacg agaagacgag acgcagaaag aacttacttc actttagaga gatcggctga
     4981 aattctgatc tctgttccgt cttgcagatt gacgacgaac accgacgatg cggtggggtc
     5041 gaactgttcg taggcaaaga gcgccgtgaa ccgtgacctg aaggcaggac cattgctttc
     5101 gttctttcgc ccccgttcgg ccttgccgat gtcgataggc ttgatccgct tcgacccttg
     5161 ctgcaattcg atcaatgcgc cctcggcgaa atactcgtcg tcgccgcaca actgcacttc
     5221 caattccatg ttgggcatgt ccatcacttt ctggatgaac tcctcgggca tgcgcacatc
     5281 tgtctttcgt tttttcgatt ctgccgcctc ttggcgacca aaggcttcga gtcgataccg
     5341 cttggtgcga aggacagcgc tcgcgccgca ggggtctttt tccggatctg cacctacgcg
     5401 ggtcgctaag gacgcctgct gaaggacttt cttcacgtct tccggagaat tggtcttttc
     5461 catcggaatt cgaccaatct ccagcgcttt gtgcgcttcg tctgacgaca gattgacgtc
     5521 aatcgcccac acgccgctgg tccctgctgt aaggactccg atcaggccca gcagtgacgt
     5581 gacctgcttg agaagagtca tgccttcctc ttatagcacc gacatggcga agtctaaagt
     5641 atccgataca atcagatgaa tggaattcat tgtaggtgaa ccatcatgcc tttgcaatta
     5701 ggtcgtgaac cgattccaat tcgtatacgg cttgcgtcga tacaggtctt cgacgttcac
     5761 gagaagagga taatcggctg accgaaggag ctgcgccaca agcttcagcg gtaaccccac
     5821 cacggtcgtg tagtcgccga tgatccgttc cacgagatct ccaccaagtc cttggatcgc
     5881 ataggctccg gctttgccaa acgactcctc cgatgcaaga taccgctcat acgcttgatc
     5941 gcgatccgct ttcatccgga cttccgccgt ggcaacctct actgattcaa tatgtcgcac
     6001 tcggtcgcat aaggcgatgg cagtatggac atgatgggaa cgaccggcca gacgtgccag
     6061 catggcgcgt gcatctgcaa gatccaccgg cttcccgagt agtcggccct cgagatcgat
     6121 cacggtatcg ctgccgagca cgagggcctg tggtcttacc agtgccacga atgtcgcctt
     6181 ttcacgcgcg aaacgcctga cctgctcgac aggcgacaac ccgaccgtgg gctgctcgtg
     6241 aaactccgac gggcacacct cgaaggaaag gcccaacagt gccagaagtt cccggcggcg
     6301 tggcgaattc gacgccagca ctagctgcat cgcgagacca gcatgctcag ctcatcggct
     6361 gattc
//
LOCUS       DATNDD010000421         4570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3927037, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000421 DATNDD010000000
VERSION     DATNDD010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3927037"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VN647_10220"
     CDS             <1..619
                     /locus_tag="VN647_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWG97448.1"
                     /translation="AWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNIHAVWQQEVWA
                     AVERSRKYINKYMGGLLTAENVEGAEAIIIASGSAAAQSREAVRLCKDKGLNVGLIKV
                     RSLRPFPTKELRELCKNTKLIVVPEFNYVGWLAKEVATAIYGYSKAKIIGGPRVYGGQ
                     SMPVELILDEVESGLTGKKSTNVAMSQVMGGAVSHDEVAHFMRSI"
     gene            complement(775..1023)
                     /locus_tag="VN647_10225"
     CDS             complement(775..1023)
                     /locus_tag="VN647_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97449.1"
                     /translation="MTDEILKAYKEVESAVERYIRLLHDHVAMLQNIEPPGSDKVVRL
                     TAGSKAMTDSAAIYLSYAKYVAYGMPSSEEMVEDEIQG"
     gene            1062..2240
                     /locus_tag="VN647_10230"
     CDS             1062..2240
                     /locus_tag="VN647_10230"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HWG97450.1"
                     /translation="MRRLNATVSPLLLDAINELVERERFSQGGLARAVAELSRRFIKD
                     RTHLSRTYLDDDLLRVAYLQYFLPVNLAKVQLLLDEMPAPEPVKGFSVLDIGSGPGTA
                     TLAVLGWWHQRKLPGALSVTAVDSSSRGLGQAGLLWDRYYRMAGIQEADLQTYVGNLE
                     RREWSEQVRQKGPFDLIILANCLNEIHTKGTDPIEAKTGLVVECLSLLTPPGTIMIVE
                     PALRETSRALHQVRDRLLQEKRCTIYSPCLHEGNCPALVKPDDWCHEERAWEPPVSIQ
                     EIDEEVGFIKDALKFSYLLLRKDGKTIVDRKPDVYRVVSELRVMKGEKRAWLCNEQGR
                     QEVGRQDRLASPHNEAFDHWHRGAIVQIERIVRKERKGKVSALGRIEPDSTVEIIRGV
                     "
     gene            2366..2641
                     /locus_tag="VN647_10235"
     CDS             2366..2641
                     /locus_tag="VN647_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HWG97451.1"
                     /translation="MLKHLRSLIRKRRYIITTHALEEMSEDDVLAGDIENAILTGQIV
                     ERQIDRVTKERKYVLTGTDCSDESVNVVLKIGPTSKAVVITVYREEE"
     gene            2638..2880
                     /locus_tag="VN647_10240"
     CDS             2638..2880
                     /locus_tag="VN647_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HWG97452.1"
                     /translation="MMQCDFCGAQGAVIKKTTKSFGRGDNLVVIENIPTIHCSSCHGS
                     YVTADTARELDHIRKNRRSLGKTKRVLVASFKKSAA"
     gene            2917..3177
                     /locus_tag="VN647_10245"
     CDS             2917..3177
                     /locus_tag="VN647_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWG97453.1"
                     /translation="MHNPPHPGEIIRQLCLEPRGVSVSEAAQALGVSRKTLSKVLNGR
                     AGISPEMAVRLSMAFGTSSSDFIRHRPCIVNRPLQETTINSL"
     gene            complement(3244..3426)
                     /locus_tag="VN647_10250"
     CDS             complement(3244..3426)
                     /locus_tag="VN647_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97454.1"
                     /translation="MSDHPKQSDLPSPEEKPKERKEIPLIAIQDDGDMMAEEMAELFE
                     EDKVSHQHGSSEPEAD"
     gene            complement(3467..3826)
                     /locus_tag="VN647_10255"
     CDS             complement(3467..3826)
                     /locus_tag="VN647_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97455.1"
                     /translation="MVTITQQEVERRLNTVPCAVCKQSSFAIDERFMGTDGDWRGICK
                     KCYYTFPVYTDMEFYLRTQPDVPLRLKEISCTACNHQGVDLDFRATLSVREAYYFVTC
                     RNCKRQFPEKSSLEAFE"
     gene            3928..4338
                     /locus_tag="VN647_10260"
     CDS             3928..4338
                     /locus_tag="VN647_10260"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HWG97456.1"
                     /translation="MHMITDNLLVGGIDDAREPPEVIGTLLFVAEEFSITPPAWVDYH
                     RIPFREFAKADPLKLMEAVQWLEPRAPKERTLVCCRAGMGRSVSVVMAYLCCIEGRTY
                     DEVLKLVMARRPGAMPLPNLQVAVERVRQLRRAA"
BASE COUNT         1045 a         1180 c         1324 g         1021 t
ORIGIN      
        1 agcctggcgt ggtgcggtcc cggtacttga tgcagagaat cctccggcgc gcctgtctcg
       61 cgacgcgccg gttcagaagt cgaacttcat ggcatacaac attcacgccg tgtggcagca
      121 agaggtttgg gcggcggttg aacgctcccg caagtacatc aataagtaca tggggggctt
      181 gctgacggcg gaaaacgtcg aaggtgcgga ggctattatc atcgcttccg gcagcgcggc
      241 ggcccaatcg cgcgaggcgg tccgcctttg caaggacaag ggcctcaatg tcggtttgat
      301 caaggttcgc tctctccgtc cgttcccaac gaaggagctg cgcgagctgt gcaagaatac
      361 aaagttgatc gtcgtgccgg aattcaatta tgtcggctgg ttggccaagg aagtagcgac
      421 cgccatctac gggtactcca aagccaagat tatcggaggg ccacgtgtgt acggcggcca
      481 atcgatgccg gtggagttga tcctggatga ggtcgaatcc gggctgaccg gcaagaagtc
      541 cacgaacgtc gcgatgtcgc aggtgatggg gggagccgtc agtcacgacg aggttgccca
      601 tttcatgcgc agtatctgag agatagggtt aaaacaggag gggggcccgt caggcatcga
      661 tgtctgacgg gcccccttcc tgtttttctg gtctgttcgt ctgctcgtct atctggtcgg
      721 ctgaataggg gaacctgaca gacaagaaag accagacaga tgatagaccg cgttctaccc
      781 ctggatctcg tcctcgacca tttcctcgct ggacggcatg ccgtaagcga catacttcgc
      841 ataggacaga tagattgccg cactgtccgt catggctttc gagccggcag tcagtcgaac
      901 gaccttgtcg gagccggggg gttcgatgtt ctgcagcatc gcgacatggt cgtgcagaag
      961 cctgatatac cgttccacgg ccgattcgac ttctttataa gccttcagga tttcatccgt
     1021 catagttgtc ctccataata ggccgagcat acactaggct catgcgacgc ctcaacgcca
     1081 cggtttcacc attactcctt gacgccatca atgaactagt tgagagagaa cgattttcac
     1141 aagggggatt ggcgcgggct gtggcggaat tgtccaggcg ctttatcaaa gatcggactc
     1201 atttaagccg aacatattta gacgacgacc ttttgagggt cgcgtaccta cagtattttc
     1261 tgccggtcaa tcttgccaaa gtccagttgt tgctcgatga gatgccggct ccggaaccgg
     1321 tcaaaggatt ttccgttctc gacattggct cgggaccggg gactgcgacg ttagcagtcc
     1381 tcggctggtg gcatcagcga aagttgcccg gtgcgttatc agtcaccgct gttgatagtt
     1441 cgtcgagagg tctcgggcag gccggactat tgtgggatcg atactatcga atggccggta
     1501 ttcaagaggc agacttacag acgtatgtgg gaaatttgga gcggagagaa tggtctgagc
     1561 aggtgaggca gaaggggccg tttgacctca tcattcttgc caactgcttg aatgagatcc
     1621 atacaaaggg gacggacccg atcgaagcaa aaactggctt agtggtggag tgtttatcgc
     1681 tgctcacacc gcccggcacg atcatgatcg tagaaccggc cttacgagaa acctcccgcg
     1741 cgctgcacca ggtgcgtgac cgattgcttc aagaaaaacg ttgcaccatt tatagtccat
     1801 gccttcacga ggggaactgt ccggcattag tgaagcccga cgattggtgc cacgaagagc
     1861 gagcgtggga accgccggtt tcgatccagg aaatcgatga agaggttgga ttcatcaaag
     1921 acgcgctgaa gttctcgtat ctattgttgc gaaaagatgg aaagaccatc gtcgatcgga
     1981 agccggatgt ctatcgcgtg gtcagtgaat tgcgagtcat gaagggagag aaaagggctt
     2041 ggctctgcaa tgaacaaggc cgacaggaag tcgggcgaca ggatcgcctg gcgtctcctc
     2101 acaacgaagc gttcgatcat tggcaccgcg gcgcgatcgt gcagattgaa cggatcgtgc
     2161 gaaaggagcg aaagggaaag gtgtcggcct tgggacggat cgagcctgac tccactgtcg
     2221 agatcatacg tggagtctga tgatcgagga gagcctggtc gggtcaattc tatgtgcccg
     2281 cacgacgcat ttcggcagca gataccaccg gtaatcttga gcctgggaat ggctgggcgt
     2341 agaatggcct ccgcatgttc gagcggtgct aaaacacctc cgttcactga tcaggaaacg
     2401 tcgttacatc atcacaaccc atgcacttga agagatgagt gaggacgacg ttttggcagg
     2461 agatatcgaa aatgccatcc tgacggggca aattgtcgag cggcagatcg atcgggttac
     2521 taaagaacga aaatatgtac ttaccggaac ggactgttcc gatgaatctg taaatgttgt
     2581 cttaaagatt ggaccaacga gtaaggcggt tgtcatcacc gtctatcggg aggaagaatg
     2641 atgcagtgcg acttctgcgg tgctcaggga gccgtcatta aaaaaacgac caaaagcttt
     2701 ggtcgtggcg acaatctcgt cgtcatcgag aacatcccga ccatacactg ctctagctgt
     2761 catgggtcct acgtcactgc cgatacagca cgcgagctcg accatattcg caagaatcgt
     2821 cgaagtttag gaaagaccaa acgggtgctc gtcgcatcgt tcaagaaaag cgcggcatag
     2881 tcagagggat ggctctatcc atccagtctc atgcgcatgc acaatcctcc acatcctgga
     2941 gaaatcatca ggcagctgtg tctggagccg cgtggagtca gtgtctcaga ggcagctcaa
     3001 gcactgggcg taagccggaa gactctgtcg aaggttctga atggccgggc gggaatcagt
     3061 cctgaaatgg cggttcgctt gtctatggcc ttcggcacgt cgtcatcgga cttcataagg
     3121 catcgtcctt gtattgtgaa ccgaccatta caggaaacta cgatcaacag cttgtgagca
     3181 ggacaggatc gctcagcgct gctcaactgc cataatcgaa tggtttggtg aaagcttgga
     3241 gggctagtcc gcttccggct ccgagctgcc gtgctggtga gagaccttgt cttcttcaaa
     3301 cagctccgcc atttcctcgg ccatcatatc cccatcatcc tgaatcgcaa tcaaggggat
     3361 ttcttttctc tccttgggtt tctcttccgg cgaggggagg tcagactgtt ttggatggtc
     3421 actcataatg ctttagtata caccggcgcc gggaacgatg gttgatttat tcaaatgcct
     3481 ccagcgacga tttttccggg aactgccgtt tgcaattccg gcaggtcacg aagtaatagg
     3541 cctctcggac ggacaacgtg gctcggaaat cgagatcaac cccctgatgg ttgcaagccg
     3601 tgcaggaaat ctcctttaac cggagcggca cgtcgggttg tgtccgcaga tagaactcca
     3661 tatcggtata caccgggaag gtgtagtaac acttcttgca gatgcctcgc cagtcgccgt
     3721 ctgttcccat gaaccgttcg tcgatcgcga agctggactg tttgcagacg gcgcatggaa
     3781 ccgtgttcag gcgacgttcc acttcttgct gagtgatggt gaccatgccg acgtgagtat
     3841 agcgggcggg gaaatgaaat cgcaactatc gggctgagcc ggcgagatgc ttgacgacca
     3901 ttagagtcgc gctatactga caacgcaatg catatgatta cggacaatct attggtgggc
     3961 ggcatcgatg acgcccgaga gcctcctgag gtgatcggga cacttctgtt tgtggccgaa
     4021 gaattttcaa tcacaccccc tgcctgggtg gactatcatc ggattccatt tcgagagttt
     4081 gccaaggccg atccacttaa gctgatggaa gcggttcaat ggcttgagcc tcgagctcca
     4141 aaagagcgga cgctggtctg ttgtcgggca ggcatgggcc gctccgtgtc cgtggtgatg
     4201 gcctatctat gctgcattga aggccggacc tacgatgagg tcttgaagtt ggtcatggct
     4261 cggcgccctg gtgccatgcc gctgccgaat cttcaagttg ccgttgaacg agtccgccaa
     4321 ctccggcgtg ccgcctagca ggctgttgga aaagtccgcc aacagcattc tcgcatcgct
     4381 cagacaaaaa ttacgactcg gcttctaaga tactgggcgc tcaccaactc gcgcccgtcc
     4441 gcaaacgtat aagattaaga cactgccggc tcaggaagac acggcccgct caccgaagac
     4501 accgccgcct caccggctcg gcggcgtcca caaacgtgac gctcattatt cttcgcgtcg
     4561 tggacctcgc
//
LOCUS       DATNDD010000422         2779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3681757, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000422 DATNDD010000000
VERSION     DATNDD010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681757"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VN647_10265"
     CDS             complement(<1..1221)
                     /locus_tag="VN647_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWG97457.1"
                     /translation="MSPLIVVPDHITQARKVEGLCPSLPWSSFADFFKSRVYDRALVN
                     RTFLTYCDDDRAVRYTYSYGEFGTVVQSVATFLHDRVGLRPGDRLATILFNHDVTVVL
                     YFAAWISGVTIVPINIEESTDKKRFILEHSEASAVCCWHRLLEEMKDLQRELPALRDV
                     IALNDDGFVEGPKHRVKAGASPFLGTASPSPSLDDEALIVYTSGTTGPPKGVVLTVVN
                     LLLDADAITEWHRFGADARLMCVLPIHHVNGTVVTVITPFYCKGSIVLNRKFKSATFW
                     RRLHEERVTCVSVVPTILEFLLDANEGIIDYKLDELAGFICGAGPLLKDTATRFEDRF
                     GFPIRHGYGLSETTCYSCFLPNDLSRDEHRHWLRDYEFSSIGVPLRHSDMAIMGEDGQ
                     LLPEMARGEICIRGG"
     gene            complement(1196..2374)
                     /locus_tag="VN647_10270"
     CDS             complement(1196..2374)
                     /locus_tag="VN647_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWG97458.1"
                     /translation="MSMVVNESPATEMKDRSAYSPGLEGVIAGESALCLVDEGEAGLL
                     YRGYPIRDLAEHSTFEEVAYLLLFGHLPSQQALSDFSARLIDQAVLPQLVQVFLGAVP
                     SGAHPMDIVRSGVSLLGVVDSDPADNSHEANVCRSIRLLAQIPLMIATSYRLITGKPL
                     RQPHDDLTFAENLLYLFTDRREDDQAKAMARVLDASLTLYAEHEFNASTFAARVTAST
                     MTDLYSAITSAIGTLKGTLHGGANEAVAEMFVDIGSCERAEAWVREALAKKRRIMGFG
                     HRVLKKGDSRSPIIQQHVESLSRICGNRRWYEIAMTIERIMEQGKGLHPNLDFYTAVA
                     YLLMEIPLTLYTPLFVCSRVAGWCAHVIEQQDHNRLMRPRALYTGPPRKEYVPLDRRT
                     "
     gene            complement(2387..>2779)
                     /locus_tag="VN647_10275"
     CDS             complement(2387..>2779)
                     /locus_tag="VN647_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWG97459.1"
                     /translation="ARGVEGLEAAVQRATTYVGAGADALFPEALESAEEFRTFAREMA
                     KGGINVPLIANMTEFGKTPLLSVAEFESLGYRGVLFPVTGLRTALQAVDRLLAELKLF
                     GSQKDWLHRMMTRRELYSLLRYTDTHER"
BASE COUNT          646 a          802 c          736 g          595 t
ORIGIN      
        1 cccgccgcgg atgcatattt ctcctctcgc catttccggc agcaattgtc catcctcgcc
       61 cataatcgcc atgtcgctat gtcgcagcgg aactccgatc gaggaaaact cgtaatctct
      121 aagccagtga cgatgctcat cacgtgacaa gtcattcggc agaaaacacg agtaacaggt
      181 cgtttcggac aagccgtacc catggcgaat cgggaacccg aatcggtctt cgaaccgggt
      241 tgcggtatct ttgagcaagg gaccggcacc gcatatgaat ccagcgagct cgtccagctt
      301 gtaatcgatg ataccctcgt tcgcatcgag gagaaactca aggatggtcg gcacgacgct
      361 cacgcaagtc actcgctctt cgtgtaatct gcgccagaac gtggcgcttt tgaatttgcg
      421 attcaggaca atgcttcctt tgcagtaaaa cggagtgatc accgtaacaa ccgtcccatt
      481 cacatgatgg atcgggagca cgcacatcag gcgggcgtct gcgccaaacc gatgccactc
      541 ggttattgca tcggcatcaa ggagtaaatt caccacggtg agaaccacac ctttgggtgg
      601 accggtggtt ccggacgtat agacgatgag cgcttcgtcg tccagactgg gactgggaga
      661 tgcagtaccc aagaagggag acgctcccgc cttgacccta tgcttcggcc cttccacgaa
      721 gccgtcatca ttcagtgcaa tcacatcgcg taaggccgga agttcacgtt gaaggtcctt
      781 catttcttcg agaagtctgt gccagcaaca gacggctgaa gcctcggaat gttccaggat
      841 aaatcgtttc ttatccgtgg actcttcgat gttgattggg acaatcgtga cacccgatat
      901 ccatgccgca aagtacagca ctacagtcac atcatgattg aagagaatcg tggccagacg
      961 gtcccctggt cttaaaccga ctcgatcatg caggaaagta gcgaccgatt ggacgacagt
     1021 cccaaattct ccataactgt aggtatagcg gacagcccga tcatcgtcgc aataggtcaa
     1081 aaatgtccgg ttgaccaagg cgcgatcata gacgcgggat ttgaagaagt ccgcgaatga
     1141 gctccatgga agtgatggac agagtccttc gactttcctc gcctgcgtga tgtggtcagg
     1201 tacgacgatc aagggggaca tattccttcc tcggcggtcc cgtgtagaga gcacgaggcc
     1261 tcatcaagcg attatgatcc tgctgttcga tgacatgggc gcaccatcct gcaacgcgcg
     1321 agcagacgaa caacggcgta tacagcgtga gcgggatctc catcagaaga taggccaccg
     1381 ccgtgtagaa atcgagattg gggtggaggc cttttccctg ctccatgatg cgctcgattg
     1441 tcatcgcaat ctcataccat cgacggttac cgcagatccg gctcaacgat tcgacgtgct
     1501 gctgaatgat gggtgaacgg ctgtcaccct tcttgagcac gcgatggccg aaacccatga
     1561 ttcgccgttt ctttgccaat gcctcccgca cccatgcttc cgcccgttca caactgccga
     1621 tatccacaaa catctcggcc acagcttcat tggctccacc atggagcgtc cccttgagtg
     1681 tcccaattgc cgaggtgatg gccgagtaca gatccgtcat cgtcgatgcc gtgacacgag
     1741 cggcgaacgt ggaggcatta aactcgtgtt cggcatacag cgtgagagag gcatcaagga
     1801 cacgggccat ggctttcgct tgatcatcct ctcttcggtc ggtgaagaga tacaagagat
     1861 tctcagcgaa ggtcagatca tcgtgcggct gccggagggg tttgccggtt atgagtcgat
     1921 aggacgttgc aatcatcagc gggatttgcg ccagcagccg gatcgatctg caaacgtttg
     1981 cctcatgcga gttgtcggct ggatccgaat caaccacgcc caacagcgag actcccgatc
     2041 ggacaatgtc catcggatgt gcgccggaag gtactgcgcc gaggaatact tggacgagct
     2101 gaggaagcac ggcctgatcg atgagtcgcg ctgagaagtc actgagcgct tgctgactcg
     2161 gcagatggcc gaacaacaac agataggcaa cctcctcaaa cgtgctgtgc tccgccaaat
     2221 cccggattgg atagccccgg tagaggagac cggcctctcc ttcatccaca aggcagagag
     2281 ccgattctcc ggcgatcact ccttcgagac cagggctata agcggaacgg tctttcatct
     2341 ctgtcgcggg agactcgttc actaccatgc tcataggtcc tcctcctcag cgttcatgag
     2401 tatcggtgta tcgaagcaga ctgtatagtt ctcgccgcgt catcatgcga tgaagccagt
     2461 ctttctgcga cccgaacaac ttcagttcag ccagcagtct atcgacggct tgcaaggccg
     2521 tccgtaaccc cgtaacagga aagagcacgc cgcgataccc caggctctcg aattctgcga
     2581 cgctcaacag aggcgttttc ccgaactccg tcatattggc gatcaatgga acattgatcc
     2641 ctcccttcgc catttcgcga gcaaatgtcc ggaattcctc ggccgattcc agagcctcag
     2701 gaaacagcgc atccgcgccg gcaccgacat atgtcgtcgc ccgttgaacg gcggcttcca
     2761 gcccttccac acctcgggc
//
LOCUS       DATNDD010000423         5940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3637712, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000423 DATNDD010000000
VERSION     DATNDD010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637712"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VN647_10280"
     CDS             <1..297
                     /locus_tag="VN647_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97460.1"
                     /translation="IAVPSYQSSLIKARETVLRQDLFTLRELLDHHRADKGKYPPSLD
                     GLVTAGYLRTLPKDPFTNSPSSWQQVIEPTEGGIFDVYSGSDLVGTNGTPYNQW"
     gene            447..1106
                     /locus_tag="VN647_10285"
     CDS             447..1106
                     /locus_tag="VN647_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWG97461.1"
                     /translation="MSDPQLTVDTLKTSLREAQRMVAELRAEVDARRQELADVQIARA
                     QLEGRIRETERRLIEARQVINLQREELSNARSEREQVGRTKSALRNQLKQLQKQLSKA
                     EKQVMGNVFPAAIAPPRDGQHELATIAPQQDARLATLEVSGAPLFDEAPVVSQSTTVP
                     SPLHVLVKSGDTLWSIARRYHTSVRRLRALNALPSDHILVGQAIRLTEPAVEMSEHER
                     M"
     gene            1151..2365
                     /locus_tag="VN647_10290"
     CDS             1151..2365
                     /locus_tag="VN647_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWG97462.1"
                     /translation="MAVFTYRVARPDGSTIHGHVEGENESLARAKLESQGLLVFSLHR
                     RGITSVKTEMSWSWGKLPLGQFLVFNQELLALVKSGLPILRIWDLLIERAGHAGFQRT
                     LRDVREDIRGGASASDALAKHPIYFPELYVATVKAGEQSGNLPEVLQRYVAYLKLMVG
                     LRQKVKKAISYPIVLIGIGMAVVGFLLAYVMPTFVSVYGESAKTLPWATQVLIDMVMH
                     AESRIVPAVVVVIGIMLGIHAYYATPTGQLAIDRLVLKLPLVGQIAVEHNTVQLTRTL
                     GTILAGGTPLVDALQGTRGAVSNRWISQETIGAVNEIREGATLADALDRFRVLPRLAI
                     EMLSVGEETGSLDAMLRDVAEFYESDLDTKLTQLTTWIEPVLLLVMGVLVGGIVIVMY
                     LPVFQMAGTVGG"
     gene            2415..4142
                     /locus_tag="VN647_10295"
     CDS             2415..4142
                     /locus_tag="VN647_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWG97463.1"
                     /translation="MPRSLTRPSLAEVLVDQRMLTKRTVEDVLRRLKGVSAALGQTLI
                     SEKLLSEDQLAHALAVQYGLAYDPLTDFRVDPRYYETISIKLMQRLPFVPIAECSGAL
                     MIAVADPQNVLGLDELELLIGKPLDRIVSTKSAILAALERSEGSSQALRELEAEYRSI
                     LVKEDDRGEEIPTLDHAEEEQSPAVKLLDSILLSAMQRRASDIHIEAADRATEVKLRV
                     DGILVPATEPLDIRLHAPLVSRLKVMSELDIAERRVPQDGSFRMRLDRKTVDFRVSIL
                     PSVFGESVVIRILDRESIATGVSDLKLERLGFNPEDLKRFRKAITRPYGMVLVTGPTG
                     SGKTTTLYAAISEMNTLEDKLITIEDPVEYQLSGVVQIPVNEKKGVTFARGLRSILRH
                     DPDKIMVGEIRDAETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDTYNFLAA
                     LNCVLAQRLVRILCPSCRTPVKGQQALIEESGLDYEQHKDTPFYEGKGCPQCHGTGYR
                     GRKCITEFLDLTDEIKEMIHAERPLAEIRYRAVTDGMITLRQSAMTKVLNGETSLREI
                     NRVTFSEEG"
     gene            4183..5142
                     /locus_tag="VN647_10300"
     CDS             4183..5142
                     /locus_tag="VN647_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97464.1"
                     /translation="MKFGTDSIAWAETERNWHGQRRHTCIMSPLSDGIIKPSPTEENV
                     SDPSALADRIRVLTRSGSVHHAVGGAVLSDLPRRIGVLLPDTAVRATVLHLEQLPVRR
                     EERDALIRWRLGQEQLFPLSGAKLLSQVFHDPSGGKGPAHTVLTVAAQESVLKQYESL
                     CESVGLIPQEVGITSLRIFDLWRRALGGSHWRSRECLWVNVSDRALTTMICQRGRLLF
                     YRCKLLGVEAIEVLTKTDMLNKILEECWASVEICQQQHPSAVVKELVICADGDISAFR
                     ELVEAELRLSVEQLGWQSVEAIGRVTKRSHRGMASLAAMAGVL"
     gene            5153..5773
                     /locus_tag="VN647_10305"
     CDS             5153..5773
                     /locus_tag="VN647_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWG97465.1"
                     /translation="MSSAVARFFEPLTSISLLRGADGQFQINLSRRYRPVVVPLRLLL
                     IGSCVLLVMGIGWNLRQAILTYQASLTIQAELDRIRQQDLDLIAEARHEGVDLTEEAL
                     KRLPFEVELANQLLKKRTFSWTKFLTELEQAIPSRLVLSSVRLDQAGTMVRLTGTAMS
                     LEDITAFTIGLRDHATFKDPILAQHRVGPNGLVEFDVTLQYRHEGT"
BASE COUNT         1357 a         1455 c         1749 g         1379 t
ORIGIN      
        1 atcgccgttc cctcctatca gtcatcattg atcaaagcga gggagacagt attgcggcaa
       61 gaccttttca cgctgcgcga gttgctggat catcaccgtg cggacaaagg gaagtatcct
      121 ccgtccttgg atggtctggt aactgcagga tatttgcgaa ctcttcccaa agaccctttt
      181 acgaattccc cgagctcttg gcagcaggtc attgaaccga cagagggtgg tattttcgat
      241 gtgtattccg gatctgacct tgttgggaca aacgggactc catataacca atggtgaata
      301 ggcacgtgag attggctctg agtagattct gccctaggct caatgtgtta cgagtgcggg
      361 gggaatgggg aagtacatca attggaatcc ttctgctcgg aatgtcggcc tgtagtccac
      421 ttgagcccat catcatagag ccggagatgt cggatcccca acttacggtg gatactctca
      481 agacctcatt gcgagaggct cagcgaatgg ttgccgagtt gagagcagag gtcgacgcac
      541 gaaggcagga attggccgat gtgcagatcg cacgggcgca gcttgaagga cgtattcggg
      601 agacggaacg tcggctgatc gaagctcgcc aggtgattaa tctccagcgg gaagaactaa
      661 gcaatgctcg ttccgaacgg gaacaggtgg ggcggaccaa atccgcactg cggaatcaac
      721 tgaagcagct tcagaagcag ttatcgaaag cagagaagca ggtgatggga aatgtctttc
      781 ccgcggctat agctcctcca cgagatgggc aacatgagtt ggcgactatt gcccctcaac
      841 aggatgccag attggccact ctggaggtgt ccggagcacc tctgttcgac gaagcaccgg
      901 tggtttcaca gtcgacaact gtgccctcgc ccttacacgt gttggtcaag tctggggaca
      961 cgttgtggag tatcgcacgc cgatatcaca cttccgtcag acgccttagg gctctcaacg
     1021 cgcttcctag tgaccatatt cttgttggtc aagccatccg gcttactgag ccagctgttg
     1081 aaatgtccga gcatgaacga atgtagtgac gcacaatggg ctaagctgca agtcgtgtag
     1141 gtctacatct atggcagtat ttacgtatcg agtcgcacgg cctgatgggt ccacaatcca
     1201 tggccatgtc gaaggagaaa atgaatcgtt ggctcgtgcc aaactagagt cccaaggatt
     1261 gctggttttt agtcttcacc gtcgaggaat cacctcagtc aagacggaga tgtcgtggtc
     1321 atggggaaag cttccgctgg ggcagttctt ggtcttcaat caagaattgc tggctctggt
     1381 caagtccgga ctgcctattt tgcgcatctg ggatctcctg atcgaacgag ccggccatgc
     1441 tggatttcaa cggacactac gtgacgtgcg ggaagacatc cgaggcggag cctcggcctc
     1501 ggatgcatta gctaagcacc ccatatattt cccggaactc tatgtcgcca cggtaaaggc
     1561 aggggagcaa tcgggcaatc ttccggaggt gctccagcgg tacgttgcat atctgaagct
     1621 gatggtcggg cttcgtcaga aagtgaagaa ggcaatctcc tatccgattg ttctcatcgg
     1681 tatcggcatg gccgtggtcg ggtttctact ggcctatgtc atgccgactt tcgtatcggt
     1741 ctatggagag tcagcgaaga ccttgccatg ggcgactcaa gttttgatcg atatggtcat
     1801 gcatgccgag tcacggatag tgcctgcagt agtcgtcgtg atcggcatta tgctggggat
     1861 acatgcctac tatgccaccc cgacgggaca gctggcgatc gatcgtcttg tgctaaagct
     1921 accactcgta ggtcagattg cagtcgaaca caataccgtc cagctgacgc gaacgctcgg
     1981 aactattctt gccggaggca ccccgctcgt tgatgccttg caaggtacac gaggggccgt
     2041 ttctaatcga tggatttcac aagaaacgat cggggccgtc aatgagattc gtgagggcgc
     2101 gacactggct gatgcgttgg atcgtttcag agtgctcccg aggctcgcaa tcgaaatgtt
     2161 atcggtagga gaggaaacgg gttcgctcga tgcgatgttg cgagacgtcg cggagtttta
     2221 tgaatctgat ctcgatacga agcttacgca actcaccaca tggatagagc cggttctgtt
     2281 gcttgtcatg ggagtgttgg tcggcggaat cgtcattgtg atgtacctac cagtatttca
     2341 gatggctggg accgtcggcg gttagatagt agagaagcgg ggtgacgaag atacaatgat
     2401 gagaggggtc gcgtatgcca cgtagcctta cgaggccatc acttgccgag gtcttggtcg
     2461 atcagcggat gctcacaaag cgcacggtcg aggatgttct ccgcagattg aagggagtgt
     2521 ctgccgcctt aggacaaacg ctgatcagtg aaaagcttct ctcagaggat caattggccc
     2581 atgccctggc tgtgcaatac ggtcttgcct atgatccgct gacggatttt cgggtcgatc
     2641 cgcgatatta cgagactatc tcgatcaagt tgatgcagcg acttcccttc gtgccgatcg
     2701 ctgaatgttc cggagcactg atgatcgccg ttgccgaccc acaaaacgtc ctcggactcg
     2761 acgaactgga gctgttgatc ggaaaaccgc tggatcgaat cgtcagtacc aaaagtgcga
     2821 ttctggcggc gctggaacgc agtgaaggat cgagtcaagc gctccgtgag ctcgaagcgg
     2881 agtatcgatc gatcttagtg aaagaagatg atcgaggaga agaaattcca actctcgatc
     2941 atgcggagga ggagcagagt ccagccgtga agctgctgga ctctatcctg ctgagtgcga
     3001 tgcagcgccg cgccagtgac atccatatcg aagccgcaga tcgcgcgacc gaagtcaaac
     3061 ttcgtgtcga cggcatcctg gttcctgcaa cggagccgct ggatattcga ttacatgctc
     3121 ctttagtgtc tcgtttgaag gtcatgtcgg agcttgatat tgccgaacgt cgggtgcctc
     3181 aggacggaag cttccgcatg aggctggatc gaaagacggt ggattttcgt gtctccattc
     3241 tgcccagtgt cttcggcgag tcggtcgtga ttcgaatatt ggatcgggaa tcgattgcga
     3301 ccggcgtgtc cgacttaaag ctcgagcggc ttgggtttaa tccggaagat ctgaagcgat
     3361 tccggaaagc gattactcgg ccctacggca tggtattggt gacggggccg acaggaagcg
     3421 ggaagacgac gacgctgtac gccgcgatat cggagatgaa cacgctcgaa gacaaattaa
     3481 ttacgatcga agatcccgtt gaataccaac tctcgggagt ggtacagatt cccgtcaatg
     3541 aaaaaaaagg agtcacgttt gctcgcggcc ttcggtccat actccgtcat gatccggaca
     3601 agattatggt cggcgaaatt cgagatgccg aaacggcgca gatcgctatc caatcggccc
     3661 tgaccggcca tcttgtgttg acgacggtgc atgcgaacaa tgtctttgac gtcatcgggc
     3721 ggttcgcgtc gatggggatc gacacctata attttctggc cgctctcaac tgtgtgttgg
     3781 cgcagcggct ggtccgaatc ctgtgcccgt catgtcggac tccggtcaaa ggacagcagg
     3841 cccttattga agaatcagga ttggactatg agcaacacaa ggatacgccg ttctacgaag
     3901 gaaaagggtg cccgcaatgc catggtactg ggtaccgagg aagaaaatgc atcacggaat
     3961 tcctcgattt gacggatgaa atcaaggaaa tgattcatgc cgagcgacct cttgcagaaa
     4021 tccggtatcg ggccgtgacc gatggaatga ttacgctgcg gcagtcggcg atgacaaaag
     4081 tgttgaatgg ggagacgtcg ctgcgagaga tcaatcgtgt gacgttcagt gaggaagggt
     4141 aatgcgatgt gggaatgggc tagtagccga ccacagcgtt gcttgaagtt cggaaccgac
     4201 tcgatcgcgt gggccgaaac cgagcgaaat tggcatgggc agcgtcgcca tacgtgcatc
     4261 atgtccccgc tttccgatgg cataatcaaa ccctctccga cggaggagaa tgtatccgat
     4321 ccctcagcat tggcggatcg gattcgtgtc ctgacgcgtt caggctcggt tcatcatgct
     4381 gtcgggggag cagtcctctc tgatcttcct cgacggattg gagtattgct gcctgacacg
     4441 gcggttagag cgacagtcct gcatctcgag caactccctg tcaggcgcga ggaacgcgat
     4501 gcgctgatcc gctggaggct tggtcaagag caactgttcc cgctgagcgg cgcgaagcta
     4561 ttgtcccagg tatttcatga tccctcggga ggcaaagggc cggcccatac tgtgctgacg
     4621 gtggcggccc aggaatcggt gctgaagcag tacgagtctc tgtgtgaatc tgtcgggttg
     4681 atccctcaag aggtgggaat cacgagccta cggatctttg atctatggag aagggcttta
     4741 ggtggatctc attggcgaag ccgagagtgt ctatgggtca acgtatcgga tcgagccttg
     4801 acgaccatga tttgtcaacg aggacgcttg ctgttctacc gatgcaagct tctgggagtc
     4861 gaggcgattg aagtgctgac gaagaccgac atgttaaaca agattcttga ggaatgctgg
     4921 gcatcggtgg agatctgtca acaacaacat ccctcagcgg tcgttaagga actggtgatt
     4981 tgtgcagatg gggacatttc ggcctttcgg gaattggttg aggcagagct ccgactgtcc
     5041 gtcgaacaac taggttggca atcggtcgaa gccattggtc gagtgaccaa aaggagtcat
     5101 cgagggatgg catcgctggc tgcgatggcc ggggtgctct agcatggcga tcatgagttc
     5161 tgcagtcgct aggttcttcg aaccactcac atcaatatca ttgctgcgcg gtgccgatgg
     5221 tcagttccaa ataaacctga gccgccggta tcggccggtg gttgttccgc ttcgattgct
     5281 cttgatcggc agctgcgtcc tgcttgtgat ggggattggt tggaacctca gacaagcgat
     5341 cctcacgtat caagcatctt taaccatcca agcagaactg gatcgaatac ggcaacagga
     5401 tctagacttg attgcagagg cccggcatga aggcgttgat ttgactgaag aggcgttgaa
     5461 gagactgcct ttcgaggtcg agttggctaa tcagctgctt aaaaaaagga cattttcttg
     5521 gacaaaattt ctgaccgaat tggaacaagc gatcccttca cgtcttgtgc tcagcagtgt
     5581 tcgccttgat caagccggca ctatggttcg actcacgggg actgccatga gcctcgaaga
     5641 tattactgct ttcaccatcg gactgcggga tcacgcaaca tttaaggacc cgatcttggc
     5701 gcaacatcgt gttgggccga acgggctggt ggaattcgac gtcacattgc aataccggca
     5761 cgaaggaact tgagcgatga aaggtcgcct gctcttcctc ctctggcagc accccttcgc
     5821 acccttgctt ccatgggtgg gagtcgttct gagtcttctt ctcatgctgt tgctcgtcca
     5881 ggatcttggt gtgaaaggcg ctcaagcgag ccgagagcgt ttggagaacg aatgggcttc
//
LOCUS       DATNDD010000424         3129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3929623, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000424 DATNDD010000000
VERSION     DATNDD010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3929623"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VN647_10310"
     CDS             complement(<1..187)
                     /locus_tag="VN647_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWG97466.1"
                     /translation="MFLSRRQFLKASAGTVAAAAVADQVLALTALQPVIEVGNPLGDY
                     PDRSWERVYHDQYRYDSS"
     gene            744..818
                     /locus_tag="VN647_10315"
     tRNA            744..818
                     /locus_tag="VN647_10315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:776..778,aa:Gly,seq:gcc)
     gene            complement(917..>3129)
                     /locus_tag="VN647_10320"
     CDS             complement(917..>3129)
                     /locus_tag="VN647_10320"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWG97467.1"
                     /translation="TSLLEHRMPFDQALRFASIWLASTSFIGLTLGAGLPEWLAVLTG
                     AALIGALLANREGKAVERLATVTPISTSGWNLLVIVGFLGFWVDMLWISGELFPAGVH
                     FLMVLMFIKLFNLKLRRDYLHLYAISLVAILASGSLTTDLWYLPIFLLYLLAGVWTLL
                     LFQLTKHSEETRMLSMSVTRRPQFPASVGHVTSQLFWLANGLALATFGLTLVIFFTIP
                     RVSAGFYQKGFGENIRTSGFSETVNLGAIGPIKRDPSVVMRVELPVSSPHEASPFYLR
                     GVAFDQYDGKSWTNRLNYRRAVSEDTTGTFVLRGSRSHASPLLGETVRQNILLEPLDT
                     PVLFAAPFIERVSGKFPNIQSDLTGSVYLPFPSSSRIEYSVLSRSKPVLPADLGSEPS
                     LYPESFTRHFLQIPGQSERIAALAREITHIQRTTYDKANAIQSFLTHNFRYSLDAPLT
                     EKGQPLEEFLFTRKTGYCEHYASAMVIMLRTIGIPARLVTGFLATEWNEYGNYYLVRQ
                     QDAHAWVEVHLPHSGWITMDPTPPSIENVGSEFPTWHALERMMDTFRLQWSRFFVQYS
                     AADQLAIVRELKAGGTSARNKVFDSIGALFSPFVTMFSGITEYASKGMILSLVELLGI
                     SLIGLAVLWLGIKRSWVREMVSKKAARDEQEIAQLYGRMVQHLAGEGISKPTTTGPLE
                     FVRIAQAKWSGANSAVASITELYCRTRFGHISLTKEELSLAEDHLRHLMALDKP"
BASE COUNT          784 a          814 c          826 g          705 t
ORIGIN      
        1 aggaggaatc gtaacggtat tggtcatgat agacccgctc ccacgagcgg tctggataat
       61 cccccagcgg attgccgact tcgatcaccg gttgcaaggc cgtcaacgcc aggacctgat
      121 ccgccaccgc cgcggccgcc accgtgcctg cagaggcttt caaaaactgt cgacgtgaca
      181 agaacattgt ggatacctcc tcaacgttaa tgagcttgaa taagcccggt tcccttgttc
      241 atttccacat tctgccggat acggaggccg gcaatagttg cttccactag ttttgagtaa
      301 cgaagggtat gggacgaagc ctttgattca ataccgatct ggttaagaaa aaacgtgcct
      361 cacccgaggc aacgaaacga actgtccgca atattcaagc catgttggtt gttgggaaca
      421 ttgttcactc atttgctatc ttaaatagat gaaaaacaat ctgtttcacc ataattattt
      481 tcttgatgtt ttaaaacgat cacgcatagt gtttatgagt agtagaggta gcatttccca
      541 tatgagagac tcataagata cggaacacgc gcttagcata ttatggtact taggtggata
      601 ttctttgcca tggtgatgta cgttacgaat atgtaacgtt atggaatttg gtaactcatt
      661 tttcctttga atcacaccgt ggtcctagcc tagcttgaca gtatcccaag aggtcagtac
      721 actccacccc tcaaacttcg cgagcgggaa tagctcagtg gtagagcatc gccttgccaa
      781 ggcgagggtc gagggttcaa atcccttttc ccgctccatc aaggttatct ttctttcagg
      841 ctggttcatc ataagtctgc cttcttccca ggcacgtatc acgatgtgta cgaaaggagg
      901 gcacggcccg attgggttac ggtttgtcga gtgccatcaa atggcgaaga tggtcttcgg
      961 cgagggacag ttcttccttt gtgagggaga tatggccgaa tcgtgttcgg caatagagtt
     1021 ccgtaatgga cgcgaccgcg gaattcgcac cactccattt cgcttgagcg atgcggacga
     1081 attcgagggg acctgtggtg gtgggtttcg aaatgccttc tccagcgaga tgttggacca
     1141 ttcgtccata gagttgcgcg atctcctgct cgtcgcgggc cgccttcttg ctaaccatct
     1201 ccctgaccca ggaccgcttg atccctagcc agagaacggc gaggccgatc agcgaaatgc
     1261 caagcaactc caccaaagac agtatcattc ctttagatgc gtattccgta atcccactaa
     1321 acatggtcac aaaagggctg aagagcgcac ctatggaatc gaataccttg ttgcgggccg
     1381 acgtacctcc cgctttcaat tcccgcacaa tggcaagctg atccgccgca ctgtactgca
     1441 caaaaaatcg gctccactgg agcctgaagg tatccatcat tcgttccaac gcatgccatg
     1501 tcggaaactc gctgccgaca ttttcgatcg agggcggagt gggatccatc gtgatccacc
     1561 ccgaatgcgg cagatgcacc tccacccacg catgggcgtc ttgctgtctg accaaataat
     1621 agttgccgta ttcattccac tcggtggcga gaaaccccgt cacgaggcgc gccgggattc
     1681 caattgtccg aagcataatc accatggctg aagcgtagtg ttcgcaatac ccggttttgc
     1741 gggtaaagag aaactcttca agcggctgac ccttttctgt cagaggcgca tcaagactat
     1801 agcgaaagtt gtgcgtcagg aacgattgga tggcgttggc tttatcatag gtcgtgcgct
     1861 ggatgtgagt gatttccctg gctagggccg caatccgctc ggattgacca gggatctgca
     1921 agaaatgtcg ggtaaatgat tcaggataga ggctaggttc cgaaccgaga tccgccggca
     1981 gtaccggctt ggatcgggaa aggacggaat attcgatccg cgacgaactg gggaacggta
     2041 aatagaccga accggtgaga tctgactgga tgtttgggaa ctttccactg accctttcga
     2101 taaagggagc agcaaagaga accggggtgt cgagcggttc caaaaggatg ttctgccgga
     2161 cggtttcgcc gaggagaggt gaagcgtgag atcgactacc gcgcaggaca aacgttccgg
     2221 tcgtgtcttc gcttaccgcc cgccggtagt tcagtcgatt cgtccaggac ttgccgtcgt
     2281 attggtcgaa tgcaacacct cgtaggtaaa atggactcgc ttcgtgcggg gagctaacgg
     2341 gcaactcaac ccgcatcacg acgctgggat cccgcttgat aggcccgatc gctcctaagt
     2401 tcaccgtttc ggagaatccg gacgtacgaa tattctctcc aaagcctttt tgataaaacc
     2461 ctgcactgac ccgtggaatc gtaaagaaga tcaccaacgt caggccaaag gtagcaagtg
     2521 caagcccgtt tgccagccaa aagagttgtg atgtcacatg gccgaccgat gcaggaaatt
     2581 gtggtcgcct tgtgaccgac atcgagagca tccgggtttc ctccgaatgt ttggtcagct
     2641 gaaacaggag cagcgtccac acgccggcca aaagatagag caagaaaata gggaggtacc
     2701 acagatccgt cgtgagggac ccggaagcca ggatcgccac gaggctgatg gcgtagagat
     2761 gtagataatc acgacgcagc ttgagattga acagcttgat gaacatgagg accatgagga
     2821 agtgaactcc tgccggaaaa agttcacccg agatccacaa catatccacc caaaatccaa
     2881 gaaagccgac gatcacgaga agattccatc cgctggttga aatcggcgta accgtggcaa
     2941 gtcgctccac agcctttcct tctctgttcg ctaagagagc accgatcagc gccgctcccg
     3001 tcagcacagc cagccattcc ggcaaacccg ctccaagcgt cagaccgatg aaggaggtgg
     3061 acgccagcca aatcgatgcg aaccgaaggg cctgatcaaa cggcatgcga tgctccagta
     3121 atgatgttt
//
LOCUS       DATNDD010000425         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_965743, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000425 DATNDD010000000
VERSION     DATNDD010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965743"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VN647_10325"
     CDS             complement(<1..1407)
                     /locus_tag="VN647_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG97468.1"
                     /translation="MNTGYDARTGRTQWVLGRGKIVSWTKDGNPLRVLGIHTDITERK
                     QLARRLAIQHEVANVLAGASGLDNAISAILQPVCQTLGWNEGLLWVVDDSTQRLRCHS
                     IWTASGTASEHYGAASRELTFQCGVGLPGRVWADAKPVWISDVTRDENFPRAALAEQV
                     GFHTAAAFPIRLADRVYAVLEFFHHEIRPADSSLLTTFQAVADQLSQFCAREHAEKRL
                     RQTQFAMDQAVDAIYWIDPQATILYANEAASLMVGYSRDELLGMRVHNLNPDFPASTW
                     PGFWEETRRNKTMSFETNHCAKDGRLVPIDIRVSFLAYEGQEFHCAFVRDITDRKLSE
                     GALAQAAQDLERKNRELAVARDKALEAAKIKAEFLATMSHEIRTPMNGVIGMTGLLLD
                     TSLTSEQREYAETVRLSSEHLLDIINEILDFSKIETGKLDLEVLNFDLHATMEEAVGL
                     LGERAYAKGLELTCLVQAG"
     gene            complement(1949..>2641)
                     /locus_tag="VN647_10330"
     CDS             complement(1949..>2641)
                     /locus_tag="VN647_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWG97469.1"
                     /translation="EADGISKAYGDNVLYESVNFSLPKGGIVGVIGPNGAGKTTMFKM
                     IIGKEKPDAGSIKVGETVKLGYVDQDRSLDGNKSVYEVISDGQDTVKLGKTEVNARGY
                     CARFNFAGTDQQKKVKDLSGGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRSLEE
                     GLDNFAGCAVISSHDRWFLDRIATHILAFEGDSTVVWFEGNYSEYEADRKKRLGKEAD
                     QPHRIRYRKLTR"
BASE COUNT          540 a          774 c          709 g          618 t
ORIGIN      
        1 acccgcctgc acgagacagg tcagctcaag gcctttggca taagcccgtt ccccgagcag
       61 gccgacggcc tcctccattg ttgcgtgcaa gtcgaaattg agtacttcca gatcaagctt
      121 gccggtctcg atttttgaga aatccaggat ctcattgata atgtccaaca gatgttcact
      181 ggaaagccgg acggtctcag catactcgcg ttgctccgag gtcagtgagg tatcaagcag
      241 caggccggtc atgccgatga cgccgttcat tggcgtccga atctcatggc tcatcgttgc
      301 cagaaactcc gctttgatct tcgcagcctc aagggcttta tcccgagcca ccgccagttc
      361 tctgttcttt cgctcaaggt cttgtgcggc ttgcgccagc gcgccctccg acagcttgcg
      421 gtcggtgatg tcgcggacga aagcacagtg gaattcctgt ccttcatagg cgaggaaact
      481 gactcgaata tcgatcggga cgagccgtcc atccttggcg caatggttgg tttcgaacga
      541 catcgtcttg tttcgccgcg tctcctccca gaagcccggc catgtggaag cgggaaagtc
      601 cggattgaga ttgtgaactc tcatgccgag gagttcatct cttgagtagc ccaccatgag
      661 gctcgcggcc tcattcgcat agaggatcgt ggcctgagga tcgatccagt aaattgcatc
      721 caccgcctgg tccatggcga actgggtttg acgcaaccgc ttctccgcgt gctcgcgggc
      781 acagaactgg ctgagctggt cggccacggc ctggaaggtg gtcaggagcg aggaatcggc
      841 cggccggatc tcatggtgaa aaaattccag caccgcatag acacgatcgg ccagtctgat
      901 aggaaaggct gcagccgtgt gaaaacccac ctgctcggcc aatgccgctc gtggaaaatt
      961 ctcatcacgg gtgacgtctg aaatccacac cggctttgcg tcggcccaaa ctcgacccgg
     1021 tagcccgacg ccgcactgaa acgtcagctc tcggctcgcg gcgccgtagt gttccgatgc
     1081 ggtgcccgac gcagtccaaa tcgaatggca gcgcagtctt tgagtcgagt cgtcgaccac
     1141 ccacagtaac ccttcattcc agcccaaggt ttgacaaacc ggttgcagga tcgcagaaat
     1201 cgcattatcc agtccggaag cgcctgccag cacgttggcc acctcatgct ggatggccag
     1261 ccgccgtgcc agctgtttgc gctcggtaat gtccgtatga atgccgagga cgcggagggg
     1321 gttgccgtct ttggtccacg aaacgatctt tcctcgtccc agcacccatt gtgtacgacc
     1381 cgtccttgca tcgtatccgg tgttcactcg tcaataccgg aatctcgccc tgaatgtatc
     1441 gctgaatgtt ggctatggcc gttgaacgat cctccgggtg gatgagagag gaccattcat
     1501 tgaaattgtc gctgacttcg tcctgtgcat atccgaacag gtctttccat ccgttagaga
     1561 aaacgacttt gttcgcggcg acgttccaat cccacagacc gatgccgctg tcttcgaaga
     1621 cgaacgtcca accatcgttg tcgatcggcg gcacccctgt ggcagcttta tcagaacgct
     1681 tactgatcat agaagcaccg cacgcggcag ggatgaaatc cgcataactc tttctcttcc
     1741 tcttcctctc gacttatatc gggttcctaa ctcaatatct tgaggggaat tgagggtcca
     1801 tttcccttgt cggaaaagcc ggatggattg gggattcaac ggtatgcagt gaacggacga
     1861 aggggagaca tagagagcca taaatgcagg ctccccaata aattctggac atggctgaca
     1921 tccatatgag ggaccgagga ggagtctcct aacgtgtcag cttccgatat ctgatcctgt
     1981 gcggctgatc ggcctccttg cccaagcgct tctttcgatc cgcttcgtac tcgctgtagt
     2041 tcccttcgaa ccagaccact gtgctgtcgc cttcaaaggc gaggatgtgc gtggcgatcc
     2101 ggtcgagaaa ccaacggtcg tggctgctga tcacggcgca gccggcgaaa ttgtccaagc
     2161 cttcttccag tgatcgcaag gtattcacgt cgagatcgtt tgtcggctcg tccagaataa
     2221 tgagattggc gccttccttc agcatgcgtg cgagatgcac acgattgcgt tcgccgcccg
     2281 agagatcttt gacctttttc tgttgatcgg ttccggcaaa gttgaagcgg gcgcagtagc
     2341 cgcgcgcatt cacttcggtt ttgccgagct tgaccgtgtc ctggccgtcc gaaatcacct
     2401 cgtacacgct cttgttaccg tcgagactcc gatcctgatc gacatagcca agcttgacgg
     2461 tctcgcccac cttgattgat ccggcgtccg gcttttcctt gccgatgatc atcttaaaca
     2521 tggtcgtctt accggcgccg tttggaccga tcacaccgac aatgccgcct ttcggcagac
     2581 tgaagttcac gctctcatac agaacattgt cgccgtaggc cttgctgatt ccgtcggcct
     2641 c
//
LOCUS       DATNDD010000426         4150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3934290, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000426 DATNDD010000000
VERSION     DATNDD010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3934290"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VN647_10335"
     CDS             complement(<1..662)
                     /locus_tag="VN647_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HWG97470.1"
                     /translation="MRYGLVLALSLFLTAAGVHCLAAADKATAAKAGQPDDPQPLIYT
                     PPKKPTPRARVGGTLRGTEGNEPELVALVPDHVGLTIKQTPTLNWYLSKPTTYPLRFT
                     LNDTQKIVPLYEGSLPVPTEAGVQSIDLKSLGITLEPNVQYRWFVSASPNPESPSRDI
                     VAGGMIERCEFSECLTITPVNLTCDRESVRTNALIGFWYDAMGCVCSLIKKDSKDPSL
                     RRL"
     gene            complement(678..2984)
                     /locus_tag="VN647_10340"
     CDS             complement(678..2984)
                     /locus_tag="VN647_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWG97471.1"
                     /translation="MRYTVLIIWFLLSVTVHLFAVTRSESHDLSSPAGSLMKEGAQAF
                     EHGAFSEALSHWKQAAELYKSAGNKPAQVEALVFSSQASMALGQSKQALQSLELALAL
                     AQKSGESLVEATLFGHLGRTYLTLRQLPEASDYLQQAASTARKNNASPLLATTLNDLG
                     ILFALKQQDNEALDAFQESVIHSQSAGLLLLATTARTNAARTLLRLGQPTHSRVALDE
                     TLDHLNDLPPSRDKSLGLIGIALAYERLLSHLPQERDPLLLRTVGVLQEAVTVAEEQG
                     DKRTLSYALGYLGHLYETEFRLDEALQLTRRALFIAQSVDAPESLYRWQWQVGRQLAA
                     TGRLDEAIASYRQAATTLQPIRVEVTHASSESPLAGQDIMKPMYFELADLLLQRASLT
                     EDGKAAEGYLLAARDAIEAYKTAELRDYFKDDCVDAVRSRLTTLDRLSSDTAVIYPIM
                     FSSRLELLMSLPTGLTRVSVPVTADRLTLEIREFRRLVEKRTTREYLPHAQQLYDWLM
                     RPLETQLVQHQITTLVFVPDSAIRTIPLAALHDGSSFLINKFALAMTPGLTLTDPRPL
                     NRDKLRFLMAGLTKSVQGFPPLPYVADEVESIKRLYKGDQLMNNAFQSSRLERELREG
                     GYGGLHIATHGKFSTDMNDSFLLTFDGKLTMQTLDQLIGLFRFRQEPLELLTLSACQT
                     GIGDDRAALGLAGVAIKAGARSALATLWFINDEASASLISEFYRQLRNPALSKAVALQ
                     RAQLKLLSDRIYEHPAYWSPFLLLNNWL"
     gene            complement(2984..>4150)
                     /locus_tag="VN647_10345"
     CDS             complement(2984..>4150)
                     /locus_tag="VN647_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HWG97472.1"
                     /translation="DPRLERINADLRPGDQRGESVLQLKVKETRPWKIWLDFNNYQTP
                     IVGAERGLITLAHQNVTGHGDPFSITYGGSRGVHPIIDTSYTVPLNRYDTTFTASYRR
                     NEFLVVSDQFRALDLNSESEIFGFTLRHPVYRSLTDELAVAITGEHLYNKITSAFDLP
                     GQPFRFIPGSSDTGVSAVSALRFIQEYQHRTASSVIALRSRVSVGLDVLGATINSREA
                     TTAGGVPLPSGRFVSWLGQVQAVKRLDSLGGWQILGRLDLQLSNNRLFPLEQIPVGGR
                     FSVRGYRENTLVRDNGFLASLESRIPLLKFASGEDMLQFAQFVDLGRAWLSRTSTPAP
                     ETLASVGLGLRWNVLPRDRARFELYWGVPLNHVSHPSGNLQDYGIHLQAVVQAL"
BASE COUNT          904 a         1136 c         1231 g          879 t
ORIGIN      
        1 agcctccgaa gcgacggatc ctttgaatct tttttaatca acgagcagac acagcccatc
       61 gcatcgtacc aaaacccgat gagggcattg gtgcgtacgc tctcacggtc acaggtcagg
      121 ttcaccggcg tgatcgtcag acactcactg aactcgcatc gctcgatcat tccaccggcg
      181 acgatatccc tggagggtga ctcaggattg gggctggccg acacaaacca gcggtattgg
      241 acgttaggtt caagtgtaat tcccaggctt ttgagatcga tggattgaac cccggcttcg
      301 gttggaacgg gaagagaacc ttcatagagc ggaacaattt tttgggtatc gttgagtgtg
      361 aaccgcaagg gataggtcgt cggtttcgag agataccaat tcaacgtcgg tgtttgctta
      421 atagtcaagc ccacgtggtc aggcaccaag gcgacgagtt ccggttcatt gccctccgta
      481 ccccgtaagg tgcctccgac tcgcgctcga ggagtcggtt tcttcggtgg tgtgtagatg
      541 agaggctgcg ggtcatccgg ttgaccggcc tttgcggcgg tcgccttgtc tgcggctgcc
      601 aagcaatgca ctcccgctgc ggtcaggaaa agactcaacg ctaggaccaa tccatatctc
      661 atggtgattc tccttaatca cagccaatta ttgagcaaca ggaatggcga ccagtaggcc
      721 gggtgttcat aaatcctgtc gctcaacagt ttcaactgcg cgcgctggag cgccacggct
      781 ttcgagagag ccggattgcg cagttgccgg taaaattccg agatcaacga cgccgacgct
      841 tcatcgttga tgaaccacag cgtggccaat gcgctgcggg cgccggcctt gatggccacg
      901 ccggccaggc caagagccgc gcgatcgtcc ccgattccgg tctgacaggc gctcagcgtc
      961 aatagttcca aaggctcttg cctgaaccga aagaggccta tcagttgatc gagggtttgc
     1021 atcgtcaatt ttccgtcgaa ggtcaggagg aacgaatcgt tcatgtcggt cgagaacttg
     1081 ccgtgcgtgg cgatatgcag gcctccatat cctccttcac gcaattcgcg ttccagtctg
     1141 gatgattgaa aggcattgtt catcagttgg tcgcccttat agagccgttt aatggattcg
     1201 acctcgtccg ccacataggg aaggggtgga aacccttgca cagatttagt gaggccggcc
     1261 atgaggaatc ggagcttgtc gcggttgagt ggacgtgggt cggtcaacgt caaaccgggt
     1321 gtcatggcga gcgcgaactt gttgatcagg aacgatgagc cgtcatggag cgccgccagc
     1381 ggaatcgtcc gaatggcgct gtccggcaca aacaccaagg tagtgatctg atgttggaca
     1441 agttgtgttt ccaacggacg catcagccag tcgtacagct gctgtgcatg aggcaaatat
     1501 tcacgagtcg tacgtttttc caccaggcgg cgaaactcgc ggatttcgag tgtcaatcga
     1561 tcagccgtca ccggcaccga gacacgcgtg agcccggtcg gcaagctcat gagcaattcc
     1621 aatcgggagc tgaacatgat gggatagatc acagcggtgt ccgacgacaa ccgatccaac
     1681 gtggtgagcc tggaccgtac ggcatcgacg caatcgtcct tgaaatagtc ccgcaactcg
     1741 gccgttttat aggcctcgat cgcatcgcga gctgcgagca gatacccttc ggctgctttc
     1801 ccgtcctcag tgagtgatgc ccgttgcagt aagaggtcgg ccagctcaaa gtacatcggc
     1861 ttcatgatgt cttggccggc tagaggactc tcggaagagg cgtgcgttac ctcgacccgt
     1921 atcggttgga gcgtcgttgc cgcttgtcgg tatgaggcaa tggcctcgtc aagccggccg
     1981 gttgcggcca gctgtcgccc cacctgccat tgccatcggt agagcgattc cggtgcatcc
     2041 actgattgcg cgatgaacaa cgcccgtctg gtaagttgca acgcttcgtc caggcgaaat
     2101 tctgtttcat acagatgacc aagataacca agagcatagg acagggttct cttgtctccc
     2161 tgctcctctg cgaccgtgac ggcttcctga agtacgccca cggttcgcaa tagaagagga
     2221 tctctttctt gaggcaggtg agaaaggagt cgctcatagg ccagagcaat gccgatcaag
     2281 ccgagtgatt tatcacgaga tgggggcaga tcatttaaat ggtcaagcgt ctcgtcgagg
     2341 gctacgcgac tgtgagttgg ttggccgagt cgcagcaggg tgcgcgcggc gttcgtacga
     2401 gcggtggtag cgagcaggag aagtccggcg ctctgtgagt ggatgacgct ctcctgaaat
     2461 gcgtccagtg cttcgttgtc ctgctgcttc aacgcgaaaa gaatgccgag atcgttcaga
     2521 gtggtggcca ggagcggcga agcgttgttt ttccgcgcgg tcgacgccgc ttgctggaga
     2581 tagtccgatg cctcgggaag ctgccggagc gtcaggtacg tccgtcccag atgtccgaac
     2641 agggtggctt ccacaagcga ttcaccgctt ttctgcgcaa gcgccagcgc cagttcaagg
     2701 gattggagcg cttgtttgga ttgccccaac gccattgagg cctgagatga gaagacgagc
     2761 gcctcgacct gtgccggctt gttgccggca cttttataca gttccgcagc ctgtttccaa
     2821 tgagacaacg cctcgctgaa ggctccatgc tcaaaagcct gagcaccttc tttcatcaac
     2881 gagccggcag gagaagagag gtcatgggat tccgatcgcg tgacggcgaa caagtgaacc
     2941 gtcaccgaca agaggaacca gatgatcaac actgtgtatc tcatcacaag gcctgcacga
     3001 ctgcctgaag atggatgcca taatcctgca ggttaccgga cggatgggat acgtgattga
     3061 gcggtacccc ccaatagagt tcaaatctgg ctcggtctcg gggcagcacg ttccaacgga
     3121 gaccaagacc gacgctggcc agcgtctctg gagccggagt gctggtgcgc gacagccagg
     3181 ctcgtcccag gtcgacgaat tgggcaaact gaagcatatc ttcaccgcta gcgaacttga
     3241 gcaggggaat tcggctttcc agcgaggcga ggaagccgtt gtctcgtacc agcgtgtttt
     3301 ctcgatagcc gcgcacgctg aaccgtccgc ccaccggaat ctgttcaagc ggaaacaacc
     3361 ggttattgct caactggaga tccagccgtc ccaggatctg ccatcctccc aaagaatcca
     3421 gccgtttcac cgcctgcact tgtccgagcc acgacacgaa gcggccgctc ggcagcggca
     3481 ctccgccggc agtggttgcc tcgcgcgaat tgatcgtcgc gcccagcaca tcgagaccga
     3541 cactgacgcg ggaacggaga gcgatcacgg atgaagcagt ccggtgctgg tattcttgga
     3601 tgaaccgcag cgcgctgacg gcgctcacgc cggtatcaga cgatccggga ataaaccgga
     3661 acggctggcc cggcaagtcg aaggccgagg tgatcttatt gtacaaatgc tcgccggtga
     3721 tcgcgaccgc cagttcgtcg gttaagcttc gatagaccgg atgccgcaat gtgaagccga
     3781 agatttccga ttcagaattc aaatccagcg cgcgaaattg gtcggagacg acaagaaatt
     3841 cattccgacg ataggatgcc gtgaaggtgg tatcgtaccg gttcaacggc acggtatagg
     3901 acgtgtcgat gatcgggtgc acgccgcgcg atccaccgta ggtgatgctg aagggatctc
     3961 catggcccgt gacattttga tgggcgaggg taatcagacc acgctccgcg cccacgatcg
     4021 gggtctgata gttattgaaa tccaaccaga tcttccacgg acgggtttct ttgaccttca
     4081 gctgtaagac gctttcgcca cgctggtcgc cgggacgaag atcggcgttg atccgttcga
     4141 ggcggggatc
//
LOCUS       DATNDD010000427         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2239124, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000427 DATNDD010000000
VERSION     DATNDD010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239124"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VN647_10350"
     CDS             complement(<1..608)
                     /locus_tag="VN647_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97473.1"
                     /translation="MNSDVRYQICVRCVMDTTDENIVFDESGICNHCKLIMDRRDNYW
                     LPDERGERKLNALLSEMKLRGQGKAYDCVLGVSGGVDSSYLLMKAHEEWGLKVLAIHV
                     DAGWNSELAVKNIELMITKLGIDLHTVVVDWEQMKDLQVAFLRAGVPNQDIPQDHAFY
                     AALYKETARQRIPFVLTGSNYATESILPSAWGYSAGDLTHIRA"
     gene            complement(712..1470)
                     /locus_tag="VN647_10355"
     CDS             complement(712..1470)
                     /locus_tag="VN647_10355"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HWG97474.1"
                     /translation="MKEIPRIIPCLLIQHGRLVKTTKFSDPKYVGDPLNAVRIFNEKE
                     VDELIFLDICASRERSEIDYDLINSIASECFMPFAYGGGLHSLDQVRRVLRNGAEKVI
                     LSSAVLEDPRLISSVADEFGSQSVVVSVNVRRKLFGAYRVFDSRKRKALSLDPIHFAK
                     RCAENGAGELFLNDVDRDGTRVGYDDALIRAVSHHVDVPVVACGGASSIEDLKHAIDV
                     GASAAAAGSLFVLHGKHRAVLITYPERDRLERLF"
     gene            complement(1467..2111)
                     /gene="hisH"
                     /locus_tag="VN647_10360"
     CDS             complement(1467..2111)
                     /gene="hisH"
                     /locus_tag="VN647_10360"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWG97475.1"
                     /translation="MIAIINYGIGNLGSIRNMIEHVGGNAVISDSAETLREARALILP
                     GVGAFDACVGALRGSGLLGLIEKRVHAEGIPLLGICVGLQLLADRSEEGREVGLGWIK
                     GDVKRLRPDPGTKLKVPHLGWRSIDVGTAGAWAGVGNTPRFYFAHSYHFVPSDKEVIA
                     GTVDYGGSVVAAVSQQNILAVQFHPEKSHRFGFQFFRQYLKELEAPSNAGWRKG"
     gene            complement(2127..>3202)
                     /locus_tag="VN647_10365"
     CDS             complement(2127..>3202)
                     /locus_tag="VN647_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97476.1"
                     /translation="DSPAIKIAVPQDEFISADLLCDFINSFDVKAVFSVSPASEWPKI
                     YRLVDRQKVQFYTVLTGYLEQQTLRKIDALQAARLDDRDIDIGYRAGGRNWRQASWLG
                     RHGILKIEIGDRVDACARRTGIRTDISSRPGDVFMGDAWYKFLLRCKYTIGLEGGASI
                     LDWDGTIRTRTLDYVEKYPDSTLEDIEAQCFPGLDGTFALFALSPRHLEACATGTCQV
                     LIEGDYNGVLEPWIHYLPLKRDFSNLAEIMLLVKQDTHRSEIVERAYQDIVQSGKYSY
                     SAFAEGVLSETLRLSSGFTQDAEFVGIDEMHELAQEEHKATNARLGKPADVSSARWYS
                     NLNLRLKQRAGRLISHAVPWLNK"
BASE COUNT          772 a          927 c          762 g          741 t
ORIGIN      
        1 gcacgaatat gagtcaggtc gccggcactg taaccccaag cactcggtag aatggattcc
       61 gtcgcgtaat tgctccccgt caaaacaaag ggaatgcgct ggcgcgcggt ctccttgtac
      121 agcgctgcat agaatgcatg atcctgcgga atatcttggt tcggtacgcc ggccctcagg
      181 aaagcgacct gcaaatcctt catctgctcc caatcgacca caacagtgtg cagatcaatt
      241 ccgagctttg tgatcatcaa ctcgatgttt ttcacagcga gttcgctgtt ccatcctgca
      301 tcgacatgaa tagcgaggac tttcagcccc cattcttcat gagccttcat caggaggtac
      361 gaactatcga ccccgccgct gacgcccaga acgcagtcgt acgcttttcc ctgccctcgc
      421 agcttcattt cggaaagaag agcatttagc tttctttctc cgcgttcgtc gggaagccaa
      481 taattgtccc gacgatccat gatcagcttg caatgattgc aaatcccgga ttcgtcaaag
      541 acgatgttct catccgtcgt atccattacg catcggacac agatctgata ccgcacgtcg
      601 ctattcatga tagtccaatt tgataggggt tggtccaagc tcatcggcag ccactacgga
      661 gctcacatca gccgggatac gccctggatg ggccaggaac ttgaggcacc gtcagaacaa
      721 ccgctccaac cgatcgcgtt ctggataggt gatcaatacg gcacgatgct ttccgtgcaa
      781 cacaaacaag ctgccagccg ccgcggcaga tgcccctacg tctattgcat gcttcaagtc
      841 ttcgattgaa gacgcgccac cacaagcaac gactggaacg tcgacgtgat gcgagacagc
      901 gcgtatcaag gcatcatcgt agccgactct tgttccatct cggtcaacgt cgttcagaaa
      961 cagctcgcca gcgccatttt ctgcacatcg cttggcaaaa tgtatcgggt caaggctcag
     1021 ggcctttctc tttcgggaat cgaacacgcg ataagctccg aagagctttc tccttacgtt
     1081 gactgacacc accacgcttt gactcccgaa ctcgtcggca acagaagaga tcagccgggg
     1141 gtcttcaagt actgcagagc tcaggataac tttttcggca ccgttccgaa ggacgcgacg
     1201 cacctgatct aggctgtgta ggcctcctcc ataggcaaag ggcatgaagc attcagatgc
     1261 gatcgaattg atcaaatcgt agtctatttc gcttcgctct cttgaagcgc aaatgtccag
     1321 aaaaatgagt tcgtcgacct ctttctcgtt aaaaatccgg accgcgttga gcggatcgcc
     1381 aacatatttc ggatcagaaa acttggtggt ttttacgaga cggccatgct gaatgagcaa
     1441 acagggaatg atccgcggta tttctttcaa ccttttctcc atcccgcgtt cgatggggct
     1501 tccaactctt tcaaatattg cctgaagaac tgaaagccaa acctgtggct cttctcggga
     1561 tgaaattgaa ccgcgagtat gttttgctga gaaaccgcgg caacgacaga tccgccatag
     1621 tcgacggtcc ctgcgatcac ttccttgtct gagggcacaa aatggtagga atgagcgaag
     1681 taaaatcgtg gggtattgcc gacccccgcc catgcaccgg ccgttccgac gtcgatagaa
     1741 cgccaaccca aatgagggac cttgagcttc gttccaggat cgggtcgaag cctcttcacg
     1801 tcacctttga tccaaccgag accaacctcc cgcccctctt cactgcgatc cgccaagagt
     1861 tgcaggccaa cacaaattcc cagaagcggg attccctctg catgaacccg tttctcgata
     1921 aggcctagta atcccgaacc gcgcaaggca ccgacacacg cgtcaaacgc cccgacgcca
     1981 ggcagaataa gcgctcttgc ttctcgcaac gtctccgcac tgtcggatat caccgcatta
     2041 ccacccacgt gttcaatcat gtttcggatc gaaccgagat ttcctatgcc gtagttgata
     2101 atcgcgatca tctaatgccc gcggatttac ttgttcagcc aaggaaccgc gtgcgagatc
     2161 agccgcccag cgcgctgctt aaggcgcaag ttcaggttac tgtaccatcg cgccgaactc
     2221 acgtcggcgg gcttgccaag acgcgcgttg gtggctttgt gttcctcttg tgcaagctca
     2281 tgcatctcat ctattccgac aaattctgcg tcttgagtaa acccggaaga cagtcggagg
     2341 gtttccgata acaccccctc cgcaaacgca ctgtaggagt actttccgga ttgcacgatg
     2401 tcctgatatg cgcgctcaac gatctcagac ctatgagtgt cttgcttcac gagcaacata
     2461 atctcagcga ggttactaaa gtcgcgcttc aatggtagat agtggatcca tggctccaat
     2521 acgccattat agtctccctc aatcagcact tgacaggtac ccgtcgcaca cgcttcgaga
     2581 tgtcgaggcg ataatgcaaa tagggcaaaa gtcccatcca accccggaaa gcattgagct
     2641 tcgatatcct ctaaggttga atccggatac ttctccacat agtcaagcgt tcgagttcga
     2701 atggtgccat cccaatctag gatactggcg ccgccttcga ggccaattgt gtacttgcat
     2761 cgcaataaga acttatacca tgcgtctccc ataaacacgt ctcccggccg ggacgaaata
     2821 tcggttcgaa taccggtcct tcgtgcacag gcatccacac gatctcctat ctcgattttg
     2881 aggatgccat gtcgtccgag ccaggaggcc tgccgccagt tcctcccgcc agcccgatag
     2941 ccgatgtcga tatctcgatc gtcaagacgc gccgcttgca gcgcgtcgat cttccggagg
     3001 gtttgttgct caaggtatcc agtaaggacc gtatagaact gcaccttctg cctgtcgacg
     3061 agtcggtata tcttcggcca ttcgcttgcg ggagaaactg agaaaaccgc cttgacatcg
     3121 aaagaattaa taaagtcgca taacaagtcg gcagagataa attcatcctg gggaacggca
     3181 atcttgattg ccggtgaatc ac
//
LOCUS       DATNDD010000428         5557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2213772, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000428 DATNDD010000000
VERSION     DATNDD010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213772"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VN647_10370"
     CDS             <1..234
                     /locus_tag="VN647_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97477.1"
                     /translation="FEPASTMEGLPMMIRRTYVVITDHAQQGGMTSCPSNSWRVQIAL
                     HYKPDVYSLTMVRADGQDVLSQSTPFRTNAIRL"
     gene            278..1312
                     /locus_tag="VN647_10375"
     CDS             278..1312
                     /locus_tag="VN647_10375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWG97478.1"
                     /translation="MSTHHLFAAHYVRLSTFGVWIATLMLTLPLNAETTPLGQDAFLR
                     ARQVTVGILADTQDQRMPEKPGKVVVRGTGFHLRDGYVITARHTADKHELATGTVIQK
                     QIRILTNDLHELPADLVGDSAFMDIVVYRVAEQHRSKLQAGTSFAADEVMPGMEVFTV
                     GYPLGWGPTMAFGRLGNTNTFLQTVDTRLIQADLSTCSGNSGGGLFNARGEIVGIVHA
                     VIQTDKEETQAHCSRMTFVIPGTLSQRIIDAALSGRPLAFSRLGVHMTSVKDGTKLRV
                     AVKEVAEPAKAAGIQKHDILLAIEGTEILDGAQLKNFLIERTRPGQEVSVKVRRVDVD
                     LTFHVVLGGG"
     gene            complement(1400..2407)
                     /locus_tag="VN647_10380"
     CDS             complement(1400..2407)
                     /locus_tag="VN647_10380"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWG97479.1"
                     /translation="MDGATNHLTSAILSAADPESIRSAGRIIKTGGIVAFPTETVYGL
                     GCDAMNADAAAKVFEAKQRPQFDPLIVHIADPKQLDVVIGSLPVLGQRLMDAFWPGPL
                     TLVFPKQRAIPDLVTAGLSTVAVRMPDHSVAQAFIREAGTPIAAPSANPFGYVSPTTA
                     QHVADGLGSKADLILDGGACTVGVESTIVSLAGLQPEILRPGSITIEQLSAVIGSIRR
                     SSPVNQTPIAPGQLARHYATRTPLTILASVGARPVVKDGEHAGLLIHSQASDADDRFA
                     AVEVLSSTGDLREAARHLFAALRRLDSLSLDRIYVEPCKEEGLGLAIMDRLRRCAAPR
                     Q"
     gene            complement(2434..2961)
                     /locus_tag="VN647_10385"
     CDS             complement(2434..2961)
                     /locus_tag="VN647_10385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWG97480.1"
                     /translation="MTMALEFRKKDPRVTISTRFGEIKIRFYSDAAPRHVENLINLVK
                     MGFYDGTTFHRVVPGFIIQGGDPLSKTPERTLHGTGGPGYFLSPEPNDRPHKRGAVSM
                     AKMPRESNSTRDFNDNGSQFFICVGENGGLDRRYTVFGEVFRGIEVVDKIVGVPRDER
                     DNPIDPIRIMMTVKE"
     gene            complement(3017..4312)
                     /locus_tag="VN647_10390"
     CDS             complement(3017..4312)
                     /locus_tag="VN647_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG97481.1"
                     /translation="MTNRTASPSPLRWLILGLLFAVSIVTYIDRVNISVTARQMMPAL
                     GLTEQEMGFVFSAFVIGYALFQIPGGWLGDRWGIRVVLMIALIWWSCFTAWTAVAATS
                     FLAVPLGIVGALALVRFLLGVGEAAALPTFNRAVTDWLPDHERGLGIGIAISGIGIGA
                     AITPPVTAWIMVNYGWQSAFYLSSGIGFALAVICWFLVADRPSLHPWCTHGDATPSTI
                     PAAPARPSIPWAILRRTPSVWWLMLSYGCLGYVAYVYMSWFYLYLVNERGFNVLRGGL
                     FAAAPFLAILVSCPLGGWVTDRLVIRRGVTRGRQLVGMIGMGLAAGSIALGAAAESPY
                     LAIASLSLGAGWLYFTVGAYWSSTSDLSPTHAGSLSGLMNMGANLGGAISPTLTPWLA
                     QQWGWGASLGLAAFISFLGGIMWLRIRPGDGLTRGGVAC"
     gene            complement(4447..5124)
                     /locus_tag="VN647_10395"
     CDS             complement(4447..5124)
                     /locus_tag="VN647_10395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWG97482.1"
                     /translation="MRIRLLCFAIGFLYLTTPLLAASPDPVNDDQKTLYALGLAISQS
                     LGTFSLSETELDMVKHGITDGVLKRPQKVDIQTFGPKIQQLQQSRLAVAAESEKKAGS
                     AFTAKAASEKGATKTESGIVITTIKPGTGATPKATDTVKVHYHGTLTDGTVFDSSVKR
                     GEPATFPLNKVIKCWTEGVQQIKVGGKSRLVCPSNLAYGDGGSPPVIKPGATLVFEVE
                     LLDIVTN"
     gene            complement(5182..5274)
                     /locus_tag="VN647_10400"
     tRNA            complement(5182..5274)
                     /locus_tag="VN647_10400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5238..5240),aa:Ser,seq:gct)
     gene            complement(5286..>5557)
                     /locus_tag="VN647_10405"
     CDS             complement(5286..>5557)
                     /locus_tag="VN647_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWG97483.1"
                     /translation="PYTAVGGFNRARKAAGLIDVTVHTLRHTFATRLIENGVDLRTVQ
                     ELGGWSSIRMLERYGHVSPSRKVAAVESLIPPNIPPLKVPNLVTA"
BASE COUNT         1286 a         1517 c         1572 g         1182 t
ORIGIN      
        1 tttgagccgg cctctacgat ggaggggctt cccatgatga tcaggagaac atatgtggtg
       61 atcacggacc atgcacaaca aggcggcatg acgtcttgcc cgagcaattc ttggcgagtc
      121 caaatcgcgt tgcactataa accggacgtc tatagcttaa caatggttcg ggctgacggt
      181 caggacgttc tgagtcagtc gacacctttc agaaccaacg cgatccgatt atagtatgca
      241 gcgccgactt gtacatgagt ttggaactga gtattgaatg agcacgcatc atctattcgc
      301 agctcactac gtgcgccttt ccacattcgg cgtgtggatc gccacgctga tgctcaccct
      361 cccgctcaat gcggaaacga cgccgctggg ccaggatgcg tttcttcgag cgaggcaggt
      421 gaccgtggga atcctggctg acacacagga ccagcgaatg ccggaaaagc cagggaaggt
      481 agttgtccga ggaaccggat ttcatctacg agacggctat gtgattaccg ccaggcatac
      541 ggcagataaa cacgagcttg cgacaggtac cgttattcag aaacagattc gcatactcac
      601 gaatgatctt catgaacttc ctgccgatct cgtgggcgac agtgcgttca tggatattgt
      661 cgtgtaccga gtcgccgaac aacatcgctc caaattgcag gcggggacgt catttgccgc
      721 ggacgaggtg atgcccggga tggaggtctt caccgtcggc tatccgctgg gctggggtcc
      781 gaccatggcc ttcgggaggc ttggaaatac caatacattc ctgcaaaccg tcgatacacg
      841 ccttatccag gccgatcttt cgacctgcag cggaaactca ggaggcgggc tgttcaacgc
      901 gcgaggagaa attgtcggca tcgtgcatgc cgtgatccaa accgataaag aggagactca
      961 ggctcattgc agtcgcatga cctttgtcat tccgggaact ctctcgcagc gcatcatcga
     1021 cgcagctctt tcggggaggc ctcttgcctt ttcaagactt ggggtccaca tgacttcagt
     1081 aaaagacggg acgaagttgc gcgtagctgt gaaggaagtc gcggaaccgg caaaagcggc
     1141 aggcattcaa aaacacgata tcttactcgc catcgaaggt acggagattc ttgacggagc
     1201 ccaactgaag aatttcctca tcgagcgtac gaggccgggt caagaggtgt cggtcaaagt
     1261 gcgtcgagtt gatgttgatc tgacgttcca tgtcgtgttg ggtggtgggt gagggcaata
     1321 agctggtagc gggtgaatgg attttcacgt gttatcggat cctcaaaagc accaagagat
     1381 tgcaaggaca tgagcggcgt cattgccgtg gcgctgcgca gcgtcgcagt cggtccataa
     1441 ttgcaagccc gagcccctct tctttgcagg gctccacata gattctgtcc agactgagcg
     1501 agtccagcct ccgcaacgct gcaaacaggt ggcgtgcagc ttccctcaga tcaccagtag
     1561 aagatagaac ttcaacggct gcaaagcgat cgtcggcatc gctggcttgc gagtggatca
     1621 gtaagcccgc gtgttcgcca tctttcacga caggccttgc tccaactgaa gctaagatcg
     1681 tcagtggcgt ccgcgttgcg tagtggcgtg ccaattgacc gggtgcgatc ggcgtttgat
     1741 tcacaggcga cgatcgtcgg atagagccga tgacggcgct gagttgttca atggtgatgc
     1801 tgcctggacg caatatttcc ggctgtagcc ctgccagtga aacaatcgtc gattcgaccc
     1861 cgaccgtgca tgctcctccg tcgagtatga gatctgcttt gcttccaagc ccatcagcaa
     1921 catgttgcgc agtcgtcggg ctcacatatc caaagggatt ggcgctgggc gctgcaatcg
     1981 gagttcctgc ttctctaata aacgcttgtg ccaccgaatg gtcgggcatt ctgaccgcta
     2041 ccgttgaaag cccggcagtg acgagatccg ggattgcccg ctgtttgggg aacaccaaag
     2101 tcagtggacc gggccaaaag gcgtccatga gccgctgacc tagaacgggc agggatccga
     2161 tcaccacatc caactgcttt ggatcagcga tgtggacgat gagtggatcg aactgaggac
     2221 gttgcttggc ttcgaagacc ttcgctgctg cgtcagcatt catcgcatcg caaccgagtc
     2281 catagaccgt ttcggttggg aacgcgacga ttccccctgt tttgatgatc cttcctgctg
     2341 accggatgga ttcgggatct gccgccgaga ggatggcact cgtcaaatga ttggtcgctc
     2401 catccatagg aatgccgcac cgacgccggt cacctactcc ttcacggtca tcatgatcct
     2461 gatcggatcg atggggttgt ctcgctcgtc gcgcgggaca ccgacaatct tgtcgacaac
     2521 ttcgatccca cgaaagactt ctccaaacac ggtatagcgt cgatctaaac caccgttctc
     2581 tcccacgcag ataaaaaatt gtgagccgtt gtcattgaag tcccgcgtac tattgctctc
     2641 acggggcatt ttggccatgg ataccgcgcc gcgcttgtgt ggacggtcgt tcggctccgg
     2701 ggaaagaaag tagcctggtc cgcccgtacc gtggagtgtg cgctcaggag ttttgctcaa
     2761 tggatcgccg ccctgaatga tgaacccggg cactacccgg tggaacgtgg tgccgtcata
     2821 gaaccccatc ttgacgagat tgataaggtt ttccacatgg cgaggtgccg catcggagta
     2881 aaagcggatc ttgatctcac cgaacctcgt ggagatcgtg acacggggat ctttctttcg
     2941 aaactcaagt gccatcgtca tgaatgtagc aaggcagtct tcatggtgac aaggccttac
     3001 caacagcact cgcatctcag cacgcaacac cgcctcttgt cagcccatcc cctggccgta
     3061 tgcgcaacca catgattcca cccaggaagg aaatgaaagc agcgagaccg agagaggcac
     3121 cccagcccca ctgttgggca agccatggcg tgagtgtcgg agaaatcgcc ccgccgagat
     3181 tggcacccat attcatgaga cctgagaggc tgccggcgtg ggtcggtgac agatcgctcg
     3241 tcgaggacca atacgcgccg actgtgaaat agagccagcc ggcacccaat gataaactgg
     3301 cgatggcgag atagggagat tctgcagcgg cacccagagc gatggaacct gctgccagcc
     3361 ccatacctat catgcccacg agctgacggc ctctcgtcac tccgcgtcga atcaccaatc
     3421 gatcagtgac ccatccgcca agagggcacg atacaaggat tgcaaggaac ggagcggcgg
     3481 caaacaatcc accgcgtaag acattgaagc cacgctcatt cacgaggtag agatagaacc
     3541 aggacatgta gacataggcg acatagccca agcatccata actcagcatc agccaccaaa
     3601 cgctcggtgt tcttcgtaga atagcccacg ggatcgaagg gcgtgctgga gctgctggaa
     3661 tcgtggatgg ggttgcgtcg ccatgagtgc accatggatg aagcgatgga cgatcggcga
     3721 ccaagaacca gcagatgact gcgagagcga atcctatccc gcttgagaga taaaaggcag
     3781 attgccaacc atagttgacc ataatccagg ccgttaccgg cggggtaatt gcggcaccga
     3841 tcccgatccc gctgatagcg atgccgatgc caagaccacg ttcatgatcg ggtaaccagt
     3901 cggtgacggc gcggttaaag gtcggtaatg ccgctgcctc tccgacaccg agaagaaatc
     3961 tgacgagggc cagtgcaccg acgattccga gcggcacggc caggaaagaa gtagcggcaa
     4021 cggccgtcca ggcggtaaaa caggaccacc agatcagtgc aatcatgagg acgacgcgga
     4081 tgccccagcg atcgccgagc cacccaccag gaatctggaa caacgcatac ccgatgacaa
     4141 atgcagagaa gacaaatccc atctcctgct cagtaagacc cagcgccggc atcatctggc
     4201 gcgccgtgac cgaaatgttg acgcgatcga tgtaggtgac gatgctgacg gcaaagagaa
     4261 gccccagaat cagccaacgt aggggcgaag gagatgcggt gcgattcgtc aaagtcctct
     4321 cgccaacaat agtcccacta tcagattcat ccagattcat tggaagggtg gtcgttccgt
     4381 ggttcggcag gcgtcgagga attgaattgc ttgccgcctc tttgcagagg cgacgaagcg
     4441 aagtggtcag ttggtgacga tgtcgagtaa ttcaacttcg aagaccaagg tggctcccgg
     4501 cttaatgacc ggcggagacc cgccgtcgcc ataggccaga ttggatgggc ataccagccg
     4561 gctctttcct ccgactttaa tctgttgaac tccctctgtc caacacttaa tgaccttgtt
     4621 caacggaaat gtcgccggct cccctcgctt gacggaacta tcgaataccg ttccatcggt
     4681 caaggtgcca tggtaatgga ccttgaccgt gtccgttgcc tttggcgtgg ctccagtgcc
     4741 gggtttgatg gtggtgataa cgattcctga ctcggtcttc gtcgcccctt tctccgaagc
     4801 cgcttttgcc gtaaacgcag agccagcctt cttctcactc tcagcggcga cggccaatcg
     4861 agactgttgc agttgttgaa tcttaggtcc aaatgtttgg atgtcgactt tttgaggacg
     4921 cttcagtacg ccgtcggtga tgccatgctt gaccatgtcc agctcggtct cgcttaagga
     4981 aaatgtcccc aacgattggc tgatggccaa cccaagagcg tacaaggttt tctgatcatc
     5041 attgaccgga tctggtgagg cagccaatag cggggtcgtg agatagagaa agcctatggc
     5101 aaaacataat aaacgaattc tcatggagta gagatccttt cttgtgttgg ctatggacaa
     5161 gcacatagga gtttgtgtgg gtggcggaga gggagggatt tgaaccctcg gtagagattt
     5221 tgtccctacg acggtttagc aaaccgttgc cttcggccac tcggccacct ctccgcaccg
     5281 atctttcaag cagttacgag attgggcact ttcaacgggg ggatattcgg ggggattaga
     5341 ctttcgaccg ctgccacctt acgcgagggg gagacatggc cgtatcgttc gagcatccgt
     5401 atgcttgacc acccgccgag ttcctggacc gttcgtaaat cgactccgtt ctcgattaat
     5461 cgcgtcgcaa aggtatgccg taaggtatgg accgtgacgt cgattagccc cgctgccttt
     5521 cgggctcgat tgaacccgcc gacggcagta tacggcg
//
LOCUS       DATNDD010000429         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2775466, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000429 DATNDD010000000
VERSION     DATNDD010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775466"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VN647_10410"
     CDS             <1..582
                     /locus_tag="VN647_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWG97484.1"
                     /translation="LTRSYDVTGGEITIDGHDLRRMPIAFLRSQIGLVQQEPFLFNGT
                     VRENLVYGNLSASHDDVVSAAKAARAHEFIVNLPEGYDTWIGERGVKLSVGQRQRVSI
                     ARVLLKDPPIVMFDEATSNIDTETEVKIREALDELTRGRTTIVIAHRLSTVHGMDRII
                     VVDHGQVVEDGPHEVLMARGGLYASLYEAQFQT"
     gene            809..1018
                     /locus_tag="VN647_10415"
     CDS             809..1018
                     /locus_tag="VN647_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97485.1"
                     /translation="MASSAGRKTVAVMRGLMMLCDSCYDQVMAEKLTDHKNFVQNYEA
                     LFDTICPGCTDINRPLVDDMLGSSE"
     gene            complement(1038..1361)
                     /locus_tag="VN647_10420"
     CDS             complement(1038..1361)
                     /locus_tag="VN647_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97486.1"
                     /translation="MPYLRTILPTFCLFYFVALASAEDKSFYSPVIQVDKDNHRVLIS
                     TLGSVFYIEVPEAGQPHMDKLPVSGLVDFVVEMRGNGQLPLIKTWKVKSGESTCMFFN
                     GKECK"
     gene            complement(1458..1970)
                     /locus_tag="VN647_10425"
     CDS             complement(1458..1970)
                     /locus_tag="VN647_10425"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWG97487.1"
                     /translation="MSDLSIPQQILDALVAHARELDPFECCGFLAGTNKTVTRVYRIK
                     NVVSLDGAAQAASFDNAKVEHLSRLSPEERAEIAFIMDAQEMFQAVKDMRKQGLTLQV
                     VYHSHPHDPARPSITDIKIATEWEENWSRLNLALPIYMLVSLMDKAHPDVRGYWIKNG
                     SVVPASILPI"
     gene            complement(1972..>2392)
                     /locus_tag="VN647_10430"
     CDS             complement(1972..>2392)
                     /locus_tag="VN647_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWG97488.1"
                     /translation="DNFTTRFLVNDACFFARKTLVSASMFRFEGQLTTIKPHAGYPCY
                     RCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGETLADKLLIYDAL
                     EMTFRKVGRPKDPACPLCGPNPSIKDLSSDYTVSCSI"
BASE COUNT          542 a          616 c          718 g          516 t
ORIGIN      
        1 ctgacgcggt cctacgatgt cacgggagga gagatcacga tcgatggcca tgacctccgg
       61 cgtatgccca tcgcttttct ccgcagccag atcgggcttg tgcaacagga gcctttcctc
      121 tttaatggga ccgtgcgcga gaacctcgta tacggcaacc tctcggccag ccatgatgat
      181 gtggtctcgg ctgcgaaggc cgcgcgcgcc catgagttca ttgtcaacct tccggaagga
      241 tacgatacct ggatcggaga gcggggggtg aagctatcgg tcggccagcg tcagcgagtc
      301 tccattgctc gtgtgctgtt gaaagaccct cccatcgtga tgttcgacga agcgacatcc
      361 aacatcgata cggaaacgga agtcaagatc agggaagcgt tagacgagct gacaaggggc
      421 aggacgacga ttgtgattgc ccaccgtctt tcgacggtgc acggcatgga ccgcattatt
      481 gtggtggatc atgggcaggt cgtggaagac ggcccacacg aagtgctgat ggcgcgaggt
      541 ggactgtatg ccagcttgta tgaggcgcaa ttccagacct aacctctgtc acaggcgcgt
      601 gaaactttat ggtcttgatc tacgaaaacg atccgctgga taatgaagat gcgcgtggaa
      661 ggttctacca tgtctgccgc gggcggattg tcaggaccga aattcaattg ccgtcaggcg
      721 agaacgtgta cgagtgcgtg gaatgtggca ttgacctcga gccggaagat ttttggatcg
      781 cgcgacagaa aggatcggtg tagacaagat ggctagcagc gcaggccgga agaccgtggc
      841 cgtcatgcgg gggctcatga tgctctgcga tagctgctat gaccaagtca tggccgagaa
      901 gctcacggac cacaagaatt tcgtccaaaa ctatgaagcg ctctttgata cgatttgtcc
      961 aggatgtacg gacattaatc gaccgctcgt cgatgatatg ttggggagtt ccgagtaagg
     1021 tacaggcaag cccgccgtta tttgcattct tttccattga aaaacatgca ggtggactct
     1081 cctgatttga ccttccacgt cttgatcagg ggcagctgac cgttgcctcg catttcgacg
     1141 acaaaatcaa ccaggccgga gacagggagc ttgtccatgt ggggctggcc ggcctccggc
     1201 acttcgatat agaatacgga cccgagggtc gaaatcagca cacggtggtt gtctttgtcc
     1261 acttgaatga caggactgta aaaactcttg tcctcggcac tggcgagagc gacgaaataa
     1321 aacagacaaa aggtgggaag tatcgtacgc aaatagggca tcacgatcac tccttcctga
     1381 aaaaagtcga agatcgcgtc attatacgca accgctcgaa aggaaggaaa ctgctatttc
     1441 tcagggcttt tcagaggcta gattggaagg atgctggcgg gaacgaccga cccgttcttg
     1501 atccaatacc cacggacgtc aggatgcgct ttgtccatga gtgacaccaa catgtagatg
     1561 ggcagggcga ggttgagccg gctccaattt tcttcccact cagtggcaat cttgatgtcg
     1621 gtgatcgacg gtctcgcggg gtcgtgggga tgcgagtggt agacgacctg cagcgtcaat
     1681 ccctgtttcc gcatgtcttt gacggcctga aacatttcct gcgcatccat gatgaaggcg
     1741 atttcggcgc gttcttccgg cgacaggcgt gagaggtgct cgaccttggc gttgtcgaag
     1801 gatgcggcct gcgcggcgcc gtccaggctg accacattct tgatgcgata gacccgtgtc
     1861 accgtcttat tcgtccccgc aaggaatcca caacattcga acggatcgag ctcccgcgca
     1921 tgagcgacga gggcgtcaag gatctgttgt ggaatgctca ggtcggacac gttagatgga
     1981 acagctgacc gtgtaatcgc tcgagaggtc tttgatgctc ggattgggac cgcagagcgg
     2041 gcacgccgga tctttgggac ggccgacctt tcgaaatgtc atctcgaggg catcgtagat
     2101 cagcagtttg tccgccagcg tttcaccgat gccgagaatc tccttgatgg cttccgaggc
     2161 ttggagaata cccattgtgc cggccagcac ccccagcacc ccggcttcct ggcaattcgg
     2221 caccaacccg gccggaggag gctcgggata caggcagcga tagcaggggt agcccgcatg
     2281 cggcttgatc gtggtcagtt gcccttcgaa gcggaacatg ctcgcagaga ccagtgtttt
     2341 tcgggcaaag aagcaggcgt cattgaccag aaagcgcgtg gtgaaattat cg
//
LOCUS       DATNDD010000430         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2467522, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000430 DATNDD010000000
VERSION     DATNDD010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467522"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="proS"
                     /locus_tag="VN647_10435"
     CDS             complement(<1..899)
                     /gene="proS"
                     /locus_tag="VN647_10435"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWG97489.1"
                     /translation="MKTSQLLIPTLRDDPGEAETVSHRLMLRAGLIRKVAAGIYTYLP
                     LGLRVIRKIEQIIREEMNRAGAQELLMPIASPAELWKETARWDYYGKELLRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQLPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDIDEVGAKVSYQKMYDAYTRIFTRCGLSFRAVEADTGLIGGDVSHEFMVLADTGE
                     ATVAYSDQGSYAANLERAEVLPPVDVEAGSLLPLTPISTPQRRTVDEVTAFLQITPRQ
                     LVKTLLYRSGEETVAVLIRGDHEV"
     gene            complement(907..2298)
                     /gene="rseP"
                     /locus_tag="VN647_10440"
     CDS             complement(907..2298)
                     /gene="rseP"
                     /locus_tag="VN647_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWG97490.1"
                     /translation="MTSVLAWSPDTLWLLLQKAWWFLVVLGVLVAFHELGHFLAARWV
                     GVKVLKFSLGFGPKLFGRQVGETEYLVSAVPLGGYVKLFGEDETEATTPDDRRRSFSH
                     QGLWGKVLIVAAGPGFNFILAYLIFAGWLSTGTPLFVPTFRDLSADVEALVPGSPASM
                     AGMEVGDRIVKVNGKDISTKTELLDIVSKSKGQTVSLEVRRADQLKTLTATPIPVTGA
                     GANGDEPLYTIGVEETPPLVTSVMHGSPAALAGFKPGDRVVAIEGQAVYTWAQMTTQV
                     REHPQKPLKLDVLRDGKRTALTVTPTSEKITVNGQTLEVGKIGISGPGRSLMRSDNAA
                     EAVYQGLEATWGWTELTAIGLYKMIVGDISSKNIGGPLTIANISGEAASQGASSVVFL
                     IAILSINLGVLNLLPIPILDGGHLLFFLIEGILRKPLGERQREVAQQVGLVLLVGVMI
                     FAFWNDLERIFSR"
     gene            complement(2365..>3027)
                     /locus_tag="VN647_10445"
     CDS             complement(2365..>3027)
                     /locus_tag="VN647_10445"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWG97491.1"
                     /translation="RRLILTASGGALWTLPQEQLLNVTPERALQHPNWKMGSKITIDS
                     ATLMNKGLEVVEARWLFNIPESNIDVLIHRESIIHSLVEYRDRSMIAQLGLPDMRTPI
                     SYAMRHPARMALDLPSLDLTEIGQLTFCKPDHNRFPCLNLGYESLRIGGTMPAAMNAA
                     NEIAVEAFLNHGLRFSEIPQVIRSTMEAHTPQEITCLDDALEADRWAREKAESLVHAL
                     PR"
BASE COUNT          689 a          838 c          830 g          670 t
ORIGIN      
        1 acttcgtgat ctcctcgaat caagaccgcg acggtttcct cccccgaccg gtacaagagg
       61 gtctttacca gttgtcgcgg tgtaatctgt aaaaaggccg tgacttcatc aaccgtccgt
      121 cgttgtggcg ttgagatcgg agtcagaggc agaagagacc ctgcttcaac gtcaacagga
      181 ggaaggactt ccgcccgttc aagattggcg gcgtaagaac cctgatcgct gtatgccacc
      241 gtcgcttcgc ccgtatcggc cagaaccatg aactcatgcg agacgtcccc accgataaga
      301 cccgtgtcgg cttcaaccgc ccgaaaggag agcccgcatc gcgtgaagat tcgggtatag
      361 gcatcgtaca ttttctgata actgaccttg gcgccgacct catcgatgtc aaaactatag
      421 gcatctttca tgatgaattc ccgccctcgc atgagcccaa atcgagggcg gatctcatct
      481 cgaaatttgg tttggatctg gtagaaattg agcgggagtt ggcgatagga acgtacttct
      541 cgccggaaga gatcggtaat aacttcttca tgcgtcgggc ctaagcaaaa atcacgctcg
      601 tgacgatcct tgaagcgtaa aagctccttc ccatagtaat cccagcgggc tgtttctttc
      661 cataattctg caggggaggc gatcggcatc aacagttcct gcgcgccggc gcgattcatt
      721 tcctcccgga tgatctgttc gattttccgg atcacacgaa gaccgagtgg aagataggta
      781 tagatgccgg ctgccacctt tcggatcagt ccggcacgca gcatcaatcg gtggctgacg
      841 gtttccgctt cgccgggatc gtcccgtagg gtgggaatga gtaactgaga ggttttcatg
      901 aaaggtttag cgggagaaga tacgttccag atcattccag aacgcaaaga tcatcacacc
      961 gaccaataat accaacccca cttgctgggc aacctctcgt tgccgttcac cgagcggttt
     1021 gcgcagaatg ccctcaatca ggaaaaagag caagtgccca ccgtcgagga tcggaatcgg
     1081 caataaattg aggacaccga ggttgatgct caagattgcg atcaggaaca cgacgctgga
     1141 ggcgccctgc gaagccgctt caccggaaat attggcgatt gtcagcggcc caccgatatt
     1201 cttgcttgaa atatctccca cgatcatttt atagaggccg atcgcggtca attcggtcca
     1261 tccccaggtg gcttccaatc cttgatacac agcctccgcc gcgttgtcgg agcgcatcaa
     1321 cgaacggccg ggccccgaga tgccgatttt tcccacttcg agagtctgtc cgttcaccgt
     1381 tattttttca ctggtcggcg tgacggtcag tgcggtacgt ttcccgtctc ggaggacgtc
     1441 gagtttcagc ggtttctgcg ggtgctctct tacctgggtg gtcatttgcg cccatgtata
     1501 gacggcttgc ccttctatcg cgaccactcg atcgcccggc ttgaatccgg ccagggcggc
     1561 gggcgatcca tgcatgaccg aggtgacgag gggaggcgtt tcttcaacac cgatcgtgta
     1621 taggggttcg tctccattgg cccccgctcc ggtaaccgga ataggagttg ccgtcagtgt
     1681 tttcagctgg tcggctcgtc ggacttcgag tgacaccgtc tggcccttgc tcttagagac
     1741 gatatccagc aactccgttt tggtcgagat gtctttgcca ttgaccttga cgatgcgatc
     1801 gccgacttcc atccctgcca ttgatgcggg agaaccggga accagggctt cgacgtcggc
     1861 actcagatcg cggaacgttg gaacaaacaa cggcgtaccg gtggacagcc atccggcgaa
     1921 gatcagatag gccagaataa aattgaaccc ggggcctgcc gccacaataa gaacctttcc
     1981 ccacaggcct tggtgcgaaa acgatcgccg acgatcgtcg ggtgttgttg cctccgtctc
     2041 atcctcaccg aacaacttga cgtacccgcc gagcggcacg gcagagacga gatactcggt
     2101 ttcgccgacc tggcggccga ataacttcgg gccgaagccg agagaaaact tgaggacttt
     2161 cactccgacc caccgcgcgg cgagaaaatg gccgagctca tggaaggcga ccaatacgcc
     2221 aagaactaca agaaaccacc aggccttctg gaggagcaac cacaacgtat caggggacca
     2281 ggcaagtacg gacgtcatca ggaactccgt gtcgcgtgta tgatcttcta ctttaacatt
     2341 cgcggcttga aatgcaaaca atcgtcagcg aggtaacgca tgcaccaatg attcggcctt
     2401 ttcacgagcc catcgatccg cttccagcgc atcatcgaga caagtaatct cctgaggggt
     2461 atgagcctcc atcgtactgc gaatgacctg cgggatttca ctgaagcgga ggccatggtt
     2521 gaggaacgcc tcgacggcga tttcgttcgc cgcattcatc gcagcaggca tcgtgccgcc
     2581 gattcgaagc gactcgtacc cgagattgag acagggaaag cgattatggt cgggtttgca
     2641 aaaggtcaat tgtccaatct ccgtcaaatc cagtgagggg aggtcaaggg ccattctggc
     2701 gggatgacgc atcgcatagg agataggggt tcgcatatcc ggaagtccca gttgggcgat
     2761 catggaccga tctctatact ctaccagaga gtgaatgatg ctctctcgat ggatcaagac
     2821 gtcgatgttg gactcgggaa tattgaagag ccagcgggct tccacaactt ccaatccttt
     2881 gttcatcaac gtggctgagt cgatggtgat tttggatccc atcttccagt tgggatgctg
     2941 cagggctcgt tccggtgtca cattcaagag ctgttcttgg ggcagtgtcc aaagggctcc
     3001 gcccgacgcc gtaagaatca atcgccg
//
LOCUS       DATNDD010000431         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2289222, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000431 DATNDD010000000
VERSION     DATNDD010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289222"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1619)
                     /locus_tag="VN647_10450"
     CDS             complement(165..1619)
                     /locus_tag="VN647_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG97492.1"
                     /translation="MNSKYAQILIVLVFVVTFFIDTVSPMGYEVWVGHIFAVLLALWV
                     ERPNTPYIVAAIATILLILEFRLSPPGIPPDMAVFNRTVGVALLWITAGLVARAKTAK
                     LDDATRRLGAIVESSSDAILSVTPDGIVTSWNAGAERMFGYTAAEMLGRSILTLVPAD
                     LHDHEARLQASVQRGQSIRTDDAVRLTKDGRSIDVSVTLSPLRDAAGRFVGVSKIIRD
                     ISERKRAQTLLQQAREALEVKVQERTAELISANHALQKLSSRLMQVQEEERSRLARDL
                     HDEVGQLLTALKIDLQEVQHSEVAQVRSDFLTDGLELVDRLLTQVRTLALNLRPSILD
                     DLGLVPALRWYANRQADRNGWTLFFSAEEMTGRVSAPIEVACFRVAQEALTNVSKYAK
                     AKTIDLTLRRSNHRVTLVIQDDGTGFDVMLARRRAQGGQTIGLLGMEERVRLAGGAFE
                     IVSAPDQGTRVELCFPLGEQDPSKQFGTVEVMVP"
     gene            complement(1776..>2600)
                     /locus_tag="VN647_10455"
     CDS             complement(1776..>2600)
                     /locus_tag="VN647_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWG97493.1"
                     /translation="QKMALGSFSAVEASVLRDIGGLAILLATWWSKEGGAIKISRADM
                     GLLGLLGLGVLGNHLLILMGLNYVSGAIGGVIIGSSPVVTALLSALLIRDVPLRAVWV
                     GALLSFAGVGLVSVAGFQAAGEQPLLGSTLVFLGVVSWALYSIGSRTIMERISALTVN
                     WTTLMVATVLQIPLLWTDQKMIHAGIGSVTTSDWLALGYLILFATAVAQQAWLFGVKG
                     IGPSRASVLGNLTPVAAVGLSALILGESVGLIEIIGIGLILAGVWVVNRQTAESNN"
BASE COUNT          601 a          817 c          691 g          491 t
ORIGIN      
        1 atccatcagc acgagatcag gagtatccgt ctgcacagac ttaacggctt cccacccatc
       61 cccgacatcg gaaacgacct caatcccttc gattttttgc aggagggccc gcataccggc
      121 tctgaccaat gcgtgatctt ccgcgatgac aacccggaga ggactcatgg gaccatgacc
      181 tcgaccgttc caaactgctt gctcggatcc tgctcaccga gcgggaagca cagttcgacc
      241 ctggtccctt gatcaggagc cgatacgatc tcgaaggcac ctccggccaa tcggacacgc
      301 tcttccattc ccagcaaccc gatcgtttgc ccgccttgtg cccgtcgtcg tgccagcatc
      361 acgtcgaatc cggtaccgtc gtcttggatg acgagcgtga cccggtgatt gctccgacgc
      421 aacgtcagat cgatggtctt cgctttggcg tacttggaga cattggtcaa ggcctcctgc
      481 gcaacccgaa aacaggccac ctcgataggg gcggacaccc gccccgtcat ttcctcggcg
      541 gagaaaaaga gagtccatcc attgcgatcc gcctgccggt tcgcatacca ccgcagcgca
      601 gggactaacc ccaagtcatc aaggatggac ggccgcagat tcaatgccag agtccgcact
      661 tgggtcagaa gacgatccac cagctcaaga ccgtccgtaa gaaaatcgga acgaacctgt
      721 gccacctcgc tatgctgaac ttcttgcaga tcgatcttca acgcggtgag caactgccca
      781 acctcatcgt gcaggtcgcg tgcgagtcgg cttcgctcct cttcctggac ttgcatcagt
      841 cgactggaca gcttctgcaa agcgtggttg gcactgatca attcggcggt tcgttcctgc
      901 accttcactt ccagtgcttc acgcgcctgt tgcagaagtg tctgcgctcg tttccgttca
      961 ctgatatcac gaatgatttt cgacacgcct acgaaccgac cggcagcatc tctgagcggc
     1021 gacaaggtga ccgacacatc gatgctccgc ccgtccttgg tcaaccgcac tgcgtcatcg
     1081 gtacggatgc tttgccctct ctgcactgag gcttgcaacc gggcttcgtg atcatggaga
     1141 tcggccggaa cgagcgtcag aatggaacga ccaagcatct cggcagcggt atacccgaac
     1201 atacgctccg ccccggcgtt ccacgaggtc acaattccgt cgggtgtcac actcaggatg
     1261 gcatcactgc tgctctccac gatggccccc agtcgcctgg tcgcatcgtc aagcttggcc
     1321 gttttcgccc gagcgacaag ccccgccgta atccagagta aagccacccc gacagttcga
     1381 ttaaacacag ccatgtccgg cgggatgccg ggaggggaaa gccgaaattc caatatgaga
     1441 agtatcgtcg caatcgctgc aacaatatag ggagtgttcg gacgttcgac ccagagagcc
     1501 aacagtacgg cgaagatatg gccgacccac acctcatatc ccatcggcga aactgtgtcg
     1561 atgaagaagg tgacgacgaa taccaaaacg atcagtattt gggcatattt actgttcatc
     1621 actatccctg actcgacgga ccacttggga tcgggatcga tgacagcgag gcgaggacat
     1681 catactatac tctccgatga ccgattgtct atggtactcc aatcccggga cagagagcga
     1741 gaccgtgcgt gaaggatggg atggagacga gaggcctagt tatttgactc agccgtttgc
     1801 cggttcacga cccagacgcc ggccaggatg aggccgatgc cgataatttc aatcagtccg
     1861 acggattctc ctagaatcaa ggccgataag ccaacggcgg caaccggcgt cagattgccc
     1921 aagaccgacg cccgtgaggg gccgatgcct ttgacgccga acaaccaggc ctgttgcgcc
     1981 actgcggtcg caaaaaggat gagatagccg agcgcaagcc aatcggatgt cgtcaccgat
     2041 ccgatcccgg catgtatcat cttttgatcc gtccacagca gcgggatctg aaggaccgtg
     2101 gcgaccatca gggttgtcca attgacggtc agtgctgaaa tccgctccat gatagtgcgg
     2161 ctgccgatac tatagagagc ccaactcacg acgccgagaa agaccaacgt gctgcccaga
     2221 agcggctgct cgccggccgc ttgaaagcct gctacggaga ccagtccgac acccgcgaac
     2281 gacagcaggg cgccgaccca cactgcgcgc aacggcacat cccgaatcaa caaggctgag
     2341 agcagagccg tcacgacggg gctcgacccg atgatcacgc cgccgatcgc cccactgaca
     2401 taattgagcc ccatgagaat gagaagatgg tttcccaaca cgccgagccc gagcaacccc
     2461 agcaacccca tgtcggcgcg actgattttg atcgcaccgc cttctttcga ccaccaggtt
     2521 gctaggagga tcgccagccc tccgatatcg cgaagaaccg aggcttcaac agcggagaac
     2581 gagccgaggg ccatcttctg
//
LOCUS       DATNDD010000432         2809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1063549, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000432 DATNDD010000000
VERSION     DATNDD010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063549"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VN647_10460"
     CDS             complement(<1..254)
                     /locus_tag="VN647_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWG97494.1"
                     /translation="MPLKGTVVLIAGGSGALGRTVTPAFAEAGARVITIERNPSPDQV
                     AAGNAMQVDVTDEAAVHRLVDEVIRTAGRLDVLVNLVGGFA"
     gene            820..969
                     /locus_tag="VN647_10465"
     CDS             820..969
                     /locus_tag="VN647_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97495.1"
                     /translation="MLQPTKAQVRATCAVMQTAHPSVGLNGDERQRDFAAGFGQGQQD
                     EEEGE"
     gene            complement(1161..1814)
                     /locus_tag="VN647_10470"
     CDS             complement(1161..1814)
                     /locus_tag="VN647_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HWG97496.1"
                     /translation="MSTPTQRAVVLFILGMVISAAGCARATQERFYTLSDSMPDGTEA
                     APIGNDFVEILPVSLPELLDRPQVVLYDEAGRVHMLEEDRWAATLKSEIRQSIGEKVR
                     HRLGMIDVYHTPVPHGESSKGKTYRITVSVESFKVAPGHGTMMQAVWAVRSIDGSTNF
                     VCRSAYQMELDDRSLSHAILAYRKAIDALGTRIAHSIRKAVQGSRDGGAEQGECIPR"
     gene            complement(1829..>2809)
                     /locus_tag="VN647_10475"
     CDS             complement(1829..>2809)
                     /locus_tag="VN647_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWG97497.1"
                     /translation="VQTPYDRFVTSETRFWHAGGVDVRMDANGVKVRTSSLAALLLGG
                     IAFDAPPDSPTTTPAEEGTTYILAVDQEQAMKRQNEKAESAVLYFDQSVRGLSPGAPV
                     DFRGLPLGDVKSIDVEFDPETREFRMPVTIEVYLERLGDRYRETILRGEPTMRTSLFE
                     AMVRRGFRAQLRTGNFLAGQLYVALDFFPHALPVLFDPNRTPREIPTVVSGIEDLQSQ
                     LAEIAQKLNKVPFDSIAANLDKSLTGLKSMVGNAEKLFRQVNEVAPEAKAALTEARKS
                     FTAAQHTLSQDAPLVRDTRQAVQELTRSAQSLRVLADYLERHPESLLRGK"
BASE COUNT          592 a          853 c          760 g          604 t
ORIGIN      
        1 gcaaagccgc cgaccaagtt caccaacaca tcgagacggc ccgccgttcg aatcacctca
       61 tccaccagac gatggacggc ggcttcatcg gtgacatcga cttgcatcgc attccccgct
      121 gctacctggt caggcgacgg attgcgctct atggtgatga cgcgcgcgcc tgcttccgca
      181 aaggccggtg tgactgtccg gcccagcgcg ccggatcctc cggcaatcag cacaactgtt
      241 ccctttaatg gcataggtct gttccctttg ggttgccgtc tctataccgt cggctcgcta
      301 tccgaactga tgagccttgc gcccgccatg cacgaccgga tagtcgggca attgtctttc
      361 gaccatgagc ctcgtcctcg cggcaggatt gattctcccg tacacgctgc gatgtccttg
      421 atgaaagttc gttcatcgcc tttctgttct atccaataca tgaggtcaga cgatctatgc
      481 aactccctcc attgcgaatc gagtcggatc gtcaagctcc gccaaagaag tgacggaggc
      541 ctgcacagca acctcgctct cgctgacgat ttcatgcctt cgtgtcgtca agaaggacgg
      601 tgagctatcc gactgtcgct gggcatcaaa cgcaaacgcg ttctgctcga acgggaggca
      661 cggacatgaa tggaccggca gcaagacaat cttcccagcc gcgccacgca atgtgaccga
      721 acaagtcgct gttatcgatt gggaaaaggt ctcaggcgat ctcgacacgc aaggcagcat
      781 tgccaatgat tgagcctctc agttatttac gggcaatagt tgttacagcc gaccaaggct
      841 caagtccgag cgacctgtgc ggtcatgcag accgcgcacc catcagtcgg cctgaacggt
      901 gatgaacggc aacgggactt tgcggccgga ttcggccagg gtcaacagga cgaggaggaa
      961 ggagaataat cctttcgacg cgagatcacg actctcgatc caacaccatc gtagtacatc
     1021 ttcgcgaagg catccgtcgg cttagtttcg ccgctcacga tgtgaatcga cggcaggaga
     1081 tcctcgggtg gaattgtaac atgcggatac gcgcgctgct ctctttcgtg atcttcagga
     1141 acctccacaa aggttcggcc tcatcgtggg atacactcgc cttgttcggc tcctccatcc
     1201 cgagacccct gcaccgcttt ccgaatactg tgcgcgatac gggtgccgag tgcatcaatg
     1261 gcttttcgat aggcaaggat agcgtggctg agactccggt cgtccaattc catttgatac
     1321 gccgagcggc agacaaagtt cgtgctgcca tcgatcgagc ggaccgccca gactgcttgc
     1381 atcatcgtcc catgacccgg agcgaccttg aacgactcga cactgacggt gatgcggtag
     1441 gttttgcctt tggacgattc cccatgaggg acaggcgtgt gatacacatc gatcatccca
     1501 agccggtgtc tcaccttctc gccgatagat tggcgaatct cgcttttgag cgtcgccgcc
     1561 catcgatctt cctccagcat atgtactcgg ccggcctcgt cgtacagcac gacctgaggg
     1621 cggtcgagta attccggcag ggatacaggc aagatttcta cgaagtcgtt cccgatcgga
     1681 gcagcctctg tcccatccgg catcgaatca gagagagtat agaaccgctc ctgggtcgca
     1741 cgggcacatc ccgcagcgct gatgaccatt ccaagaatga acaggacgac agcccgttga
     1801 gtcggtgtgc tcacttcaac tccgctttct attttcctcg aagtaaggac tcaggatgac
     1861 gctcgagata atcggccaag acccgcaacg attgggcgga gcgagtcagt tcttgcacgg
     1921 cttgccgcgt atcgcgcaca agcggcgcat cttgcgacaa ggtgtgctga gccgcagtga
     1981 acgattttcg cgcttcggtg agtgccgctt tggcctccgg cgccacctca ttgacttgcc
     2041 tgaacaattt ctccgcattc cccaccatgc tcttcaaccc tgtcaggctt ttgtcgagat
     2101 tcgcggcgat gctgtcgaac gggaccttgt tcagcttttg ggcgatctcg gccaactgag
     2161 actgcaggtc ttcaattccg ctgacgaccg tgggtatctc gcgaggggtt ctgtttggat
     2221 caaacagaac cggcaacgcg tgcgggaaaa agtcgagagc aacatagagt tgacctgcca
     2281 aaaaatttcc cgttcgcagt tgggcgcgaa agccgcggcg gaccatggct tcaaataacg
     2341 acgttctcat ggtcggctcg ccccttagaa tcgtctcacg ataccgatcg ccgagacgct
     2401 caaggtaaac ttcaatggtg accggcatcc ggaattctct tgtctccggg tcgaactcga
     2461 cgtcgatcga tttcacgtcg ccgagcggca ggccacgaaa atcgaccggc gcacccggac
     2521 tcagtccccg gacggactgg tcaaaataca ggacggccga ctccgccttc tcgttctgcc
     2581 gtttcatggc ctgctcttgg tctacagcta gaatataggt cgtaccttcc tccgccggag
     2641 tcgttgtcgg gctgtccggc ggcgcgtcaa aagcgatccc gccaagcagc agcgcggcca
     2701 gcgacgaagt cctcaccttg acgccgttcg cgtccatcct gacatccaca ccaccggcat
     2761 gccagaagcg cgtctcggat gtcacaaaac ggtcataggg agtctgcac
//
LOCUS       DATNDD010000433         3588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1066964, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000433 DATNDD010000000
VERSION     DATNDD010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066964"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..1401
                     /locus_tag="VN647_10480"
     CDS             475..1401
                     /locus_tag="VN647_10480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWG97498.1"
                     /translation="MKAIRLHQRGGPESLLYYEDTPRPIQKDNQVLVQVYATAITPTE
                     FDWYPTFHTPEGGMRPFPIILGHEFSGVVEAVGSDCLGIQVGDPIYGLSDWFIDGAQA
                     EYCLTVPANIAPKPVTLEHIQAAAVPISALTAWQALIDRAHLSSGQRVLIHGAAGGVG
                     SFAVQVARHQCAHVIATASAANIDFVKALGADEVIDYRITPFETVVRDVDVVIDTVGG
                     DTRDSSWEVLRNGGRLVTIAADAEGAKEQRVRDAFFIVESNRNQLIDISRLIDTGVLR
                     PAVGVVYSMENFRQAYEQKPGRGKNVLRIAEK"
     gene            1437..1880
                     /locus_tag="VN647_10485"
     CDS             1437..1880
                     /locus_tag="VN647_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWG97499.1"
                     /translation="MMTIAPIRTVTRQTLQWMALAGATLLLGQTALAEDSVSVLIRQR
                     LADMAGKEGTVITVDYAPSAASSQHFHPGSVFAYVLEGAVVSQLGGEQPVTYTKGQSW
                     YESPKEPHIVSKNASKTEPAKLLVFLLSQESESLVVPMKSLGSGK"
     gene            2178..2681
                     /gene="msrA"
                     /locus_tag="VN647_10490"
     CDS             2178..2681
                     /gene="msrA"
                     /locus_tag="VN647_10490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWG97500.1"
                     /translation="MSRTETAILAGGCFWGMQDLIHKLPGVLSTRVGYSGGDVSNATY
                     RNHGTHAEAIEVVFDPDMLEFRGLLEVFFQIHDPTTVNRQGNDQGTSYRSGIYYTSEA
                     QRMVAEETITDVNASGLWPGKVVTEVRPAGPFWQAEPEHQNYLERYPNGYTCHYARPN
                     WKLPRRA"
     gene            2892..3122
                     /locus_tag="VN647_10495"
     CDS             2892..3122
                     /locus_tag="VN647_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWG97501.1"
                     /translation="MQDTTITAKQRQVKEVSTRVVKAAAVQMSPVLYCREGTVEKVLR
                     KIHELGRQGVQFATFPETVIPYYPYFSFVQPR"
     gene            3131..3301
                     /locus_tag="VN647_10500"
     CDS             3131..3301
                     /locus_tag="VN647_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97502.1"
                     /translation="MRRLSVVSSRPFDEIVTRLTATIGHPDMDAFHKASVAARTIADL
                     ENVAKERSDRQI"
BASE COUNT          852 a          892 c         1076 g          768 t
ORIGIN      
        1 caagacggaa aaaagcgaca cccctagcct atgggcagcg tcctgcccat cgctgggtgt
       61 tgaagggggt gtagagtagc cttcggaagt gcgttaagtc aaggcgagat caggggggaa
      121 ctggctacga tgggttcgtc tacacagtca ttcgatcgtg ttcgtggagt atcagccttt
      181 cggcgtagca gcgagatcga gtctccggtc attttgacca gatcaggaag agatccggca
      241 ttcatttttt gcatcaccct gcctcggtgt gctttaacag taatctcgct gatgccgagt
      301 tcacccctaa tctgtttatt caattaggtg gtcgtttagt tcagtgagtt catgagaagg
      361 gggaaaacgt attgatcaat acagacgtcc gaattgaagg tcgattgagg gatcacgaat
      421 aatggcagac ttccgtgccc gcatggagta aggctccgcg atgaggttct tcccatgaaa
      481 gcgatacggt tacatcaacg tggtggcccc gagtcgcttc tctattatga ggatacaccg
      541 agaccgattc agaaggacaa tcaagtgctc gttcaggtct atgccacggc gatcacacca
      601 acggagtttg actggtatcc aacattccat actcctgaag gtgggatgag accctttccg
      661 attattctgg gtcatgaatt ctcgggtgtt gtcgaagcag tcgggtcgga ttgcctgggt
      721 attcaggtgg gggatcctat ttacggactg agcgattggt tcatagacgg tgcgcaggcg
      781 gaatattgtt tgaccgttcc tgccaacatt gcaccgaagc ctgtcacgct tgaacatatt
      841 caagccgcgg cggtcccgat ctcagcactc accgcttggc aggcgcttat cgatcgcgca
      901 cacctctcgt cagggcaacg ggtgttgatc catggtgcgg ccggtggtgt gggcagcttt
      961 gcagtgcagg tggctcggca tcagtgcgca cacgtgattg caacggcttc ggcagcgaac
     1021 atcgattttg tcaaggcgct cggcgccgat gaagtcatcg attaccggat cacgccgttt
     1081 gaaacggtcg tcagagatgt ggatgtagtg atcgatactg ttggaggaga cacgagagat
     1141 agttcgtggg aagtccttag aaacggtggt cgcttagtca ccatcgcggc agatgccgaa
     1201 ggggcgaagg agcagcgggt gcgcgatgcc ttcttcatcg tcgaatcgaa ccggaatcaa
     1261 ctgatagaca tttcgcgtct gatcgatacc ggcgtcctcc gaccagccgt gggtgtcgtg
     1321 tactcgatgg agaactttcg acaggcctac gaacagaaac ctgggcgcgg caagaatgtt
     1381 ctccgcatcg cggagaagtg atggatggat cataatgaac aagaaggagc acgccaatga
     1441 tgaccattgc acctatccgc actgtgacaa gacagacact tcaatggatg gccctggcag
     1501 gggcgacgct gcttctggga cagacagcgc tggcagaaga tagcgtgtcg gtgctgataa
     1561 ggcagcggtt ggcggatatg gccggcaaag aaggcactgt gattaccgtg gactatgcac
     1621 cgagcgccgc atccagccag cacttccatc cgggctcggt gttcgcctat gtcttggagg
     1681 gagccgtggt ctctcaatta ggaggagagc agcccgtgac ctatacgaaa ggtcagagct
     1741 ggtacgagtc tcccaaggaa ccgcatatag tatccaagaa cgcgagcaag accgaaccgg
     1801 ccaagttgct ggtgtttctg ctgtcacagg agagcgagtc gctcgtcgtg ccgatgaaat
     1861 cactcggcag cgggaagtga accgatttat ggtacctgaa gcacgtgcct cagctgacta
     1921 ctgaacgggg gtttcaccca acccgtggcc ccgagcgtga tcgcctatca tcaggacgac
     1981 agtcctggta tgcgccggat cgagacaacg tgcgatgtgt gcgatgctca tcttggccat
     2041 gtgtttccag atggacctga accaagcggg ttacggttct gtatcaatgc ccttttgttg
     2101 cggaaggctg aggggtgatc cgccgaaata gacggcttcc gacgcaggga caggcacacg
     2161 acgaaaggag tatttccgtg agccgaactg aaacagcgat attggccggc ggttgtttct
     2221 ggggcatgca ggatcttatc cacaagctcc ccggtgtgct ttctacgcgc gttggctaca
     2281 gcggtggtga cgtgtcgaat gcaacctatc gtaaccatgg gacgcatgcg gaagccatcg
     2341 aggtcgtgtt cgacccggac atgctggagt tccgtggcct actggaagtg tttttccaaa
     2401 ttcacgatcc tacgaccgtg aaccgccagg gtaacgatca aggaacatca taccgttcgg
     2461 gaatctatta cacctcggaa gcccaacgca tggtggcaga agagacgatc accgacgtta
     2521 acgcgtctgg gctgtggccg ggaaaagtag tcacagaagt ccggccagcc ggtccgttct
     2581 ggcaggcgga accggaacat cagaattatc ttgaacgata tcccaacgga tatacctgtc
     2641 actacgcgag acccaactgg aagttaccgc ggcgggcctg aggtacaacg cctttgctgc
     2701 acgcgctgct ctacgagctg catgagggga gaataaatct ggcccacgcc ccgatggtgg
     2761 ctgcgaatga gttcaaggat aaagttgcac aggtagcagt aaaacacttc agataagaag
     2821 gaggttgaaa atcatctcaa gaaagccatg cttcaagacc gaactgtttg agtatgaagg
     2881 ggatagcgaa aatgcaagat acaaccatta cggcaaagca gcgtcaggtg aaggaggttt
     2941 cgacgagagt cgttaaagcc gcagcggttc aaatgagccc cgtgctctat tgccgcgagg
     3001 gcacggtcga aaaggtcctg aggaagattc acgagcttgg tcggcagggg gtgcagttcg
     3061 ccacgttccc ggagactgtc atcccttact atccgtactt ttcctttgtc caaccgcgat
     3121 agaggtccaa gtgcgaagat tgagcgtcgt ctcgtcgagg ccattcgacg agatcgtcac
     3181 caggctcacg gcgaccatcg gccatccgga catggacgca tttcacaaag catctgtcgc
     3241 ggctaggacc atcgcggatc tggaaaacgt agcaaaggag cgatcggatc gtcagatctg
     3301 atggagttca tccgctttga cgtcggcgaa gtcctaagca aggaacgaga cggccacggg
     3361 caaagattct ccggctcgtc gtcggtaatc cgctcatcat gaaggagatg gcgaagaccg
     3421 ttccggacgc cgcgattcag aagacggact ttcgctgcac gaagaagtag tggtggctga
     3481 tgtatcggag tcgaccataa ctgatgatgg ggggcgtcac ccttcaaatc gtaggaacca
     3541 acgggggctg tgcacagtga tggcgcatgg tggcaaggac gtggccta
//
LOCUS       DATNDD010000434         3819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4218874, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000434 DATNDD010000000
VERSION     DATNDD010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218874"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..301
                     /locus_tag="VN647_10505"
     CDS             161..301
                     /locus_tag="VN647_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97503.1"
                     /translation="MIPVLHDVRAAFFLVIALAMLLFICAVTGLGAVLRPIRWSDRKG
                     YR"
     gene            625..1260
                     /locus_tag="VN647_10510"
     CDS             625..1260
                     /locus_tag="VN647_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWG97504.1"
                     /translation="MNGVLAKCGAVVLMGLVLLSNQGCNKKWLQSDGEGKAESGSSKL
                     PNMSGGGSSNELSGFSRNPSEERLAQGGYSTALNPSGFSARQRAELTKEERAAVEAGL
                     QDVFFGYDQWTLSDVGTEALNQDAAYLKDHPGALLKIEGHCDERGTADYNMVLGDKRA
                     KAARNYLAEVGVNPKQVAIVSYGKERPFCFDRDESCYQQNRRSHMLLTGEK"
     gene            1370..1750
                     /locus_tag="VN647_10515"
     CDS             1370..1750
                     /locus_tag="VN647_10515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97505.1"
                     /translation="MKKLGSFFKTETIDRTPSKPQPQDRRVQPRFTAQFRSTFSGQRQ
                     EGQGRTLDVSAGGCKIESDMKVEQGAKFECRLHIPGLDWPLRIDEATVRWVDGNSFGI
                     AFSRITLEECAKLKTILSELEQDE"
     gene            1981..2142
                     /locus_tag="VN647_10520"
     CDS             1981..2142
                     /locus_tag="VN647_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97506.1"
                     /translation="MEQRYAITVKFLIDADSAEDAEEMVETACEKASASFPEMSYEGI
                     EDIEEEEEG"
     gene            2194..2757
                     /gene="msrA"
                     /locus_tag="VN647_10525"
     CDS             2194..2757
                     /gene="msrA"
                     /locus_tag="VN647_10525"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWG97507.1"
                     /translation="MRDESGPISSGEEIAILAGGCFWCLEALYDQVLGVDSVESGYIG
                     GHLDHPTYEAVCGGQTGHAEAVRIIFDSRVITYKGLLEIFFAIHDPTTPNRQGNDKGT
                     QYRSAIFFCDATQRHTAETLIKSLTEEKLYDAPIVTQVVPAGRWYEAETYHQEYFVRN
                     PFQGYCQFVVGPKVAKFRKLFAHRLKT"
     gene            complement(2769..3200)
                     /locus_tag="VN647_10530"
     CDS             complement(2769..3200)
                     /locus_tag="VN647_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97508.1"
                     /translation="MTTASTNDESNRTEPPLWSIGIAVVVILLAPLVLYSWAPTGPIR
                     EGDTVFSEGQQHVQIRLTATGQDAKDNTCLLDSNSPLIVVHSLNTDTDGSIIAHVQGN
                     PAGEWPFCPVHTEVLLKTHQVFQKPAVFETVREILVGLFSR"
     gene            3409..>3819
                     /locus_tag="VN647_10535"
     CDS             3409..>3819
                     /locus_tag="VN647_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97509.1"
                     /translation="MIRPFLKTIALGFVVQSALHLACFAEPSTAELSGPLAAVTVDPT
                     LPHYAARTQVSGAFKVHGSDTMSLLLARLSMEFQRLQPKAAIEVRGGGSVKAIAELLQ
                     PPLSKTGKVMLFEERAAHVQLIATSRELRDAEVKA"
BASE COUNT          930 a          928 c         1037 g          924 t
ORIGIN      
        1 gacgattgga gcggtatact ttgaacagaa gaagcggttt gcctggacga gacaatgtcc
       61 ctgttgtgga agaccgcttg agaagatgga cccgatgagt ccgtgggtct gtgaatgtgg
      121 atggcgcagt caatgaatcg agcaagaaga gggaggtacc atgattcccg ttttgcacga
      181 cgtgagagcg gcgttttttc tggtaatcgc actagccatg ctgttgttta tctgtgcggt
      241 gaccggctta ggagcagtgc tgcgaccgat ccgatggtct gatcggaaag ggtatcgctg
      301 agcaagagag gggcaagcca gtagtggggc gtgatcggtc gctttctctt cttgctagta
      361 tttttcgttt cttgctgatc cgatttcagt tctctgtcta gtagagaatc tttccctctc
      421 tcctccggtg tctgctcaaa tatttgaatt tattaggcat cctgtcttcc ttcggctacg
      481 ctccagctaa agcgtccctt ttcgactgcg gtgctatatt tgtttggaag gggaaccagc
      541 gtgcattgca gaccatcgtc atgcatcgat tgtctaggaa ttagttgaac agcggcgtcg
      601 tgatatcacc tggaggagaa gatcatgaat ggagtgctgg cgaaatgtgg agcggtggtg
      661 ctaatgggac tggtcctatt gtcgaatcaa ggctgtaaca aaaagtggct ccaatctgac
      721 ggtgaaggta aagccgagag cggaagttcg aagcttccaa atatgtcagg aggaggttca
      781 agcaatgaat tgagcggttt ttctcgtaat ccgtcagagg aacgacttgc gcaaggcgga
      841 tattcgacgg cgctgaaccc ctcagggttc agcgcacgtc agcgtgccga gctgacaaaa
      901 gaagaaaggg ctgccgtgga agcgggtttg caagatgttt tctttggtta tgatcagtgg
      961 accctgtcgg atgtcggcac ggaagcgctt aatcaagacg ctgcttatct caaggaccat
     1021 ccaggcgccc tgctgaaaat tgaagggcac tgtgacgaac gagggaccgc tgactataac
     1081 atggttttgg gcgacaaacg cgccaaggct gcccgaaact accttgcgga agtcggggtg
     1141 aatccgaagc aggtagcgat cgtatcgtat ggcaaagagc gtcccttttg tttcgatcgg
     1201 gatgagtctt gctatcagca gaaccgccgc agccacatgc ttctgacggg cgaaaagtaa
     1261 gacgttttat ggtcagacat catccgcatg gagcacgggt ttcttccgag cgtttacaaa
     1321 aatctgtcgc atcacctcgg atcgttaatt tcgggcaagg aattcttcgg tgaaaaagct
     1381 cggttctttc ttcaaaacgg aaaccatcga tcgaactccg tccaaacctc agccacagga
     1441 ccgacgcgtc caaccaagat tcaccgcgca atttcgcagc accttttcgg gtcagagaca
     1501 ggaaggacag gggcgaaccc tggatgtgtc tgccggaggt tgtaaaatcg aaagcgacat
     1561 gaaagtggag caaggtgcga agttcgaatg ccgactccac attccaggcc ttgattggcc
     1621 gttgcggatc gacgaagcca ccgtgcgttg ggttgatggc aatagttttg ggatcgcgtt
     1681 ttcgcgtatc actctggaag agtgtgcgaa actcaagacg atactctctg aacttgaaca
     1741 ggatgagtaa attccttccc tagtcgctac aacgcacaca cttccgccct cgtaaggatt
     1801 tcttgaagac ttgctcgatg ggatcgtatc gatctcgcct tccatctgtg tgtccatgcg
     1861 ctttcccaag atgctatcct gaacacggtt gacatggcta ccgtaactgt cagaaagtac
     1921 ggattagttg gtgtctgaat ccaaaacatc tcggcatgag gagagagaaa ggagtcggga
     1981 gtggaacaga ggtatgcaat taccgtgaaa ttcttgatcg atgccgattc ggcggaagat
     2041 gctgaagaga tggtcgaaac tgcctgtgaa aaggcgtcgg cctcattccc agaaatgagt
     2101 tacgaaggga ttgaagatat tgaagaggaa gaagaagggt agtcggtctt catctagcga
     2161 cggtatccca ccataatact cgatatatct acgatgagag atgaatctgg accgatctcc
     2221 tctggcgagg aaatcgccat tcttgcaggt ggctgttttt ggtgtctcga ggccctctat
     2281 gatcaggtcc taggtgtgga ttccgtcgag tctggttaca tcggaggtca cctagaccac
     2341 cctacgtacg aagctgtgtg cgggggacag accggccatg ccgaagccgt acggatcatc
     2401 tttgattctc gagtaatcac ctacaaagga ctgcttgaga tattttttgc catccatgat
     2461 ccgacgacac ccaatcggca agggaacgat aagggaaccc aataccgttc ggcgattttc
     2521 ttctgtgacg caacacagcg tcacacagcc gagaccttga tcaaaagcct aactgaagag
     2581 aagctgtacg acgctcctat cgtcacgcag gttgtaccgg ccgggcgatg gtatgaggcg
     2641 gaaacctatc atcaggagta tttcgttcga aatcccttcc aaggttactg tcagtttgtg
     2701 gtgggtccaa aagtcgccaa gtttagaaaa ctattcgctc atcggctaaa aacttaagcc
     2761 gtacccgctc atcgactgaa caatccaacc aagatctccc gcaccgtctc aaagaccgca
     2821 ggcttctgga atacttgatg cgttttcaac agcacctcgg tatgcacggg gcaaaaaggc
     2881 cattcgccgg ccgggttgcc ctggacgtgg gcaatgatag atccgtctgt gtcggtattg
     2941 agtgagtgaa cgactatgag aggactgttc gaatcaagta aacaggtatt gtctttagcg
     3001 tcctggcctg ttgctgtaag gcgaatctga acgtgctgtt gtccctctga gaatacagta
     3061 tccccctccc gtataggtcc ggtcggcgcc catgaataca gaaccagcgg agcaagcaaa
     3121 atgacgacta cggcaatgcc gatcgaccag agtggaggct cagttctatt actctcatcg
     3181 tttgtagagg ctgtagtcat cttctgatcg actcttctca tttaattagg accgactgta
     3241 gccggtgcta tggattttgt gagacgtgac gaccactctc ttgttcttgc cgcatcagat
     3301 tactaagaaa aatttaacag tcctgtaata ccgcggttac cctctcgcaa tcctgcgcga
     3361 ttacaacgaa gccgacagca atggttacct taaccaggga ggaacccaat gatacggcca
     3421 tttttgaaaa cgatcgcgct tggatttgtt gtgcagtccg cgcttcatct tgcctgcttt
     3481 gccgaaccat caacggctga actgagtggt cctctagccg ccgtgaccgt tgatccgact
     3541 ctgccccact acgcggcgcg aacacaggtg tccggcgcgt tcaaagttca tggatcagac
     3601 acgatgtctc tgcttctcgc tcgcctcagc atggagtttc agcggctcca acccaaggcc
     3661 gccattgagg tgcgcggagg aggctccgtc aaagccattg cggaacttct gcagccccct
     3721 ctcagtaaaa cgggcaaggt gatgttgttc gaagaacgcg ccgcccatgt tcaactcatc
     3781 gccacatcgc gtgaacttcg cgatgccgaa gtgaaagca
//
LOCUS       DATNDD010000435         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_490893, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000435 DATNDD010000000
VERSION     DATNDD010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490893"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VN647_10540"
     CDS             <1..267
                     /locus_tag="VN647_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97510.1"
                     /translation="RYEARSSGSVHYKLFGATPVMIDELKGHVTEACLSDLTGQLVRD
                     TKLYMASSAPLPDTGIRTVTVKAKRPAGTQPTRSLVPVATATTP"
     gene            359..673
                     /locus_tag="VN647_10545"
     CDS             359..673
                     /locus_tag="VN647_10545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG97511.1"
                     /translation="MNIDAFRQMVAKNPKGFLGRYGLGNKILQENGSVEEAVEHLTVA
                     TQLDPTHAASHLALGRALIRVGRDADAKRILTAGIEAVLSGRSNGGKDLVPEMQELLR
                     SL"
     gene            706..906
                     /locus_tag="VN647_10550"
     CDS             706..906
                     /locus_tag="VN647_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97512.1"
                     /translation="MNLDDARKRIESAVTQYGQHAAPAIDLVMAEVRSDLGKGAFNEL
                     IEEFDLELMYNITPMESDQSNS"
     gene            959..2056
                     /locus_tag="VN647_10555"
     CDS             959..2056
                     /locus_tag="VN647_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97513.1"
                     /translation="MPLIWAAISGHGFGHAAQVVPVLNALHRFVPDLRVLLRTAVPPS
                     FFQDRLVPPWDVSPVQQDVGCVQNGPLKIDVEATWREHHRFHSSWEERLQAEFEAMKT
                     AGPNVVLADTPYLALAAGKTAALPAVALVSFTWDLVLSEYIAPRSIDGRAIIQSIRQA
                     YGKADLALRINPAPKMEIFQRIIDIGPIAEPASPAREQLTEFLGLARGEQTVLVGFGG
                     VPLDSLPFSRLESLTGYRFIFDGSIPLGSRRFVAAASLPFSFKTLMASIDLIMTKPGY
                     GTLVEAVALRTPLVYVRRYNFADEQPLVDYLHRYGRGVELSLDAFAHGHWEESLQRAQ
                     SLPPPSEHVPPATGADEAAALLAPYFFTARH"
     gene            2200..>2476
                     /locus_tag="VN647_10560"
     CDS             2200..>2476
                     /locus_tag="VN647_10560"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HWG97514.1"
                     /translation="MNLGTVYAKNPDYVQRDVAGECILVPIRRNLTESNSIYVLNETG
                     AALWGRINGLRSVQTISHEFVQEYDVTVEQLHQDFHTLLIDLLSIHAV"
BASE COUNT          524 a          740 c          650 g          562 t
ORIGIN      
        1 cgctacgaag cccgttcctc cggttcggtg cattacaagt tgttcggcgc cacgccggtc
       61 atgatcgatg aactcaaggg acacgtaacg gaagcctgtc tcagtgatct gacgggacaa
      121 cttgttcgtg acaccaaact ctatatggcc agcagcgccc cgctcccgga caccggcatt
      181 cgcacggtaa cggtgaaagc caaaagacca gccggcacac agccaacccg ttctcttgtt
      241 cccgtcgcga cggctacgac gccttaggaa cacggagttc cccccaacca acgttctcat
      301 ctggccgatc ctgccgttcc agtggtatag tgacgcctgg gcactggaac cacgattgat
      361 gaatattgac gcttttcgtc agatggtcgc taagaatccc aagggattcc tcggccgtta
      421 tggactcggc aataagatcc ttcaggaaaa cggtagcgtc gaagaagcag tcgagcatct
      481 tactgtcgcc acacaattgg acccgacgca tgccgcctct catctggcgt taggacgcgc
      541 gctgatccga gtgggcaggg acgccgatgc caagcgtatt cttaccgccg gtatcgaggc
      601 cgtactttca ggacgatcga acggcggcaa ggatctggtg cctgagatgc aggagctgct
      661 acgctcgcta tgacgtatga acttgtacca taaaggagtt tcgccgtgaa tctcgacgat
      721 gccagaaaac gaattgaatc agccgtcaca caatacggcc aacacgccgc ccccgccatc
      781 gacctcgtca tggcagaagt tcgctccgat ttgggaaagg gagcgttcaa tgaactgatt
      841 gaagagttcg atcttgagtt gatgtacaac atcactccga tggaatcgga ccagtcaaat
      901 agttgaacag tatcttccta ttccaccgtt cactcgtgac tcgcacagtc tcctgcttat
      961 gccgctgatc tgggctgcca tctcaggaca tggatttggt cacgccgcgc aagtcgtgcc
     1021 cgtgctcaat gcattacatc gtttcgttcc tgaccttcgc gtcctcctgc gcacggctgt
     1081 ccctccttcc ttcttccaag accgtctcgt tcctccgtgg gacgtaagcc ccgttcagca
     1141 ggatgtcgga tgcgttcaga atggcccgct gaagatcgat gtagaagcca cctggcgcga
     1201 gcaccaccgg tttcacagtt cttgggagga acggcttcag gcggagttcg aagcgatgaa
     1261 gaccgccggc ccgaatgtcg ttctggccga tactccttac ttggctttgg cggccgggaa
     1321 aacggctgct cttcctgccg ttgcgctcgt cagttttacc tgggatctcg tgctgtccga
     1381 gtatatagcc cctcgatcaa tcgacggccg ggcaatcatc caatcgattc gacaggccta
     1441 cggcaaagca gatctcgcgc tgcggatcaa tccggctccc aaaatggaga tcttccaacg
     1501 catcatcgac attggaccga tcgccgagcc tgcatcgccc gctcgggaac aattgaccga
     1561 gttcctaggg ctggcccgtg gagaacaaac cgtgctcgtc ggatttggtg gagttcccct
     1621 ggactcactt ccattcagcc ggctggaatc actcaccggg tatcgtttca tcttcgacgg
     1681 ctcgattcca ctgggaagtc gtcgattcgt cgctgccgca tccctgcctt tttcattcaa
     1741 gaccttaatg gcgtctatcg atctcatcat gacgaaaccg ggctacggca ccttggtgga
     1801 ggcagtcgcg ctacgcactc ctctggtata tgtccgtcga tacaatttcg ccgatgagca
     1861 gccactggtt gactatttgc accggtacgg acggggcgtt gaactgtcac tcgatgcttt
     1921 cgctcatggt cattgggagg agtctttgca acgtgcgcaa tcgcttcccc ctccctccga
     1981 gcacgttcct ccggctacgg gcgccgatga ggcggcagcg ctcctcgctc cctatttttt
     2041 cacggctaga cattgaccgg tgcgtgtacg gataaccggc gcgaccatct tcgaattgaa
     2101 ctggaatgaa gggtttgccg tgatcgtcta atcagacccc agaagctgcc gcgaaatatc
     2161 tctagcgggt ccctaaggga tttgctatcc tgccgcctga tgaatctcgg caccgtgtac
     2221 gcaaagaatc ctgattatgt gcagcgtgac gtggccggcg aatgcattct cgtccccatc
     2281 cggaggaacc tcaccgaatc caacagcatc tacgtcctga atgagacggg agcggccctc
     2341 tgggggcgga tcaacggact tcgatccgtt caaacgatca gccatgaatt cgttcaggag
     2401 tacgacgtga cagtggaaca actccatcag gattttcaca ccttgctgat cgacctgctc
     2461 tcgatccatg ccgtca
//
LOCUS       DATNDD010000436         2023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2552007, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000436 DATNDD010000000
VERSION     DATNDD010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2552007"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VN647_10565"
     CDS             complement(<1..269)
                     /locus_tag="VN647_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97515.1"
                     /translation="MSNPVPPPQSGFLAKTLGTMMSDPPWAAKSFLAAGATTVAGLGA
                     WLSNMMSPALASAGGSFLGGFLLGWAFRKTLKLALLVTISLLALIV"
     gene            complement(266..1126)
                     /locus_tag="VN647_10570"
     CDS             complement(266..1126)
                     /locus_tag="VN647_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWG97516.1"
                     /translation="MRIDDQRESDNIEDRRGMGPARIGKGGLGIGTIVLALAVSYFTG
                     VNPLTVLNLLTGVQSMTEVSAPSEPGPVGVPQDRLGKFASVVLADTETTWRSLLGPRY
                     KDPRLVLFSGAVQSACGTTSSAVGPFYCPNDHRVYLDLAFFDEMAHRLGAAGDFAQAY
                     VIAHEIGHHVQNLLGVAEKVHRLQRQVSEVEGNALSVRMELQADCYAGVWGHHADRER
                     NLIEPGDFEEGLRAAAAIGDDRLQKMSRGHVQPESWTHGSSEQRMTWLKRGLESGDPS
                     VCNTFETNRL"
     gene            complement(1271..>2023)
                     /locus_tag="VN647_10575"
     CDS             complement(1271..>2023)
                     /locus_tag="VN647_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HWG97517.1"
                     /translation="FLPVCSSVMMQCPIVASQDRRSSSPHPRVEVIMLRVILAVMLLL
                     LSFGLSSCDKAYMATMEKMGYAKRDILSSRVKSARDAQEDAKKEIQSTLEQFGKVVSY
                     EGGDLEDIYKKLSGELESSEDSAKSVRKRISDVESVANALFSEWEEELGQYSSADLRR
                     KSQAKLTQTKGRYREMLGAMKRAEQRIEPVLEPLRDQVLYLKHNLNARALAAIKGELV
                     KVDAQVDQLVKDMNRSISEADRFIQSMEKESD"
BASE COUNT          423 a          569 c          573 g          458 t
ORIGIN      
        1 acgatgagtg ccagcaatga gatggtgacc aacagggcga gctttagggt tttccggaat
       61 gcccatccaa gcaagaatcc cccgaggaag ctgccaccag cgctcgcgag tgccggcgac
      121 atcatattac tgagccaggc gccgagtccg gcgacagtcg tcgcaccggc ggcgagaaac
      181 gatttagccg cccagggagg gtcggacatc attgtcccca gtgtcttagc aaggaagccg
      241 ctttgaggtg gaggaactgg attgctcata gccggtttgt ctcgaatgtg ttacagaccg
      301 agggatcgcc ggattcaagg ccgcgcttaa gccaggtcat ccgttgttca gatgagccgt
      361 gggtccagct ttccggttga acatgaccac gcgacatctt ctgtaagcga tcatctccga
      421 tggctgctgc cgcgcgcagc ccttcttcga aatcgcccgg ctcgatcaag tttcgctcgc
      481 gatcggcatg atgaccccag acaccggcat aacaatcggc ttggagttcc atccggaccg
      541 aaagggcgtt tccttccacc tcagaaacct gtcgttggag gcgatgcact ttttctgcga
      601 cgccaaggag attttgcacg tgatggccga tctcatgggc gatcacatag gcttgagcaa
      661 agtcacccgc cgctccaaga cgatgcgcca tttcatcgaa gaaggcaaga tccaaataga
      721 cccggtgatc gttggggcaa tagaacggtc caacagccga cgaggtggtg ccgcatgccg
      781 actgcacggc gccggaaaag aggacaagcc gtgggtcctt gtaccgagga ccgaggaggc
      841 ttcgccaggt cgtttcggtg tctgcaagga cgacggaggc aaacttgccg agtcggtctt
      901 gagggacgcc gacaggaccg ggttcagatg gggcggacac ctcggtcatg ctctgaacac
      961 cggtgaggag attcagcacg gtgaggggat tgacgcccgt gaaatagctg acggcgagtg
     1021 ccaacacaat ggtgccgatg ccgaggccgc ctttcccgat gcgggccgga cccatgccgc
     1081 gacgatcttc gatgttgtcg ctttcacgtt ggtcgtcaat gcgcataggc gctttccctc
     1141 aggcaggatg ttgaaaaaga ccatcagctt cgttctcgca tcgctcaagg tctcaacgta
     1201 ccaaccgcgt acgcctcgac ctttcgctcg ctgcggcctt gctggaggcc tttttgaaca
     1261 ttctgcaagc tcagtcggac tctttttcca ttgattgaat aaatctatcg gcttcggaga
     1321 tggaacggtt catgtctttg accagctgat cgacctgcgc gtcgactttg acgagttcgc
     1381 ccttaatggc ggcgagtgcg cgtgcgttca ggttgtgttt caaatagagc acctgatctc
     1441 gcaatggttc aaggaccggt tcaatccgct gctcggcccg tttcatggcc ccgagcatct
     1501 ctctgtagcg acccttggtc tgggtgagtt tggcttggct tttccggcgc aggtctgcgc
     1561 tcgagtattg accaagctct tcttcccatt ccgaaaagag tgcattggcc acactttcga
     1621 catccgagat tcgtttccgg accgatttgg cactgtcctc gcttgactcg agttcaccgc
     1681 tcagcttctt atagatgtct tccagatctc ctccttcata ggagaccacc ttgccgaatt
     1741 gctccagcgt gctctgaatc tctttcttcg cgtcttcttg ggcatcccgt gccgatttga
     1801 cgcggctgct caggatatcg cgtttggcat agcccatttt ctccatggtg gccatatagg
     1861 ccttgtcaca agaggacagg ccaaatgaca ggagcagcag catgacagct agaatcactc
     1921 gcaacatgat gacctctact ctcggatgtg ggctcgacga gcggcgatcc tgagacgcca
     1981 cgatgggaca ttgcatcata actgaagagc atacagggag aaa
//
LOCUS       DATNDD010000437         5963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2217538, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000437 DATNDD010000000
VERSION     DATNDD010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217538"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            483..2993
                     /gene="uvrA"
                     /locus_tag="VN647_10580"
     CDS             483..2993
                     /gene="uvrA"
                     /locus_tag="VN647_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWG97518.1"
                     /translation="MGNSIIIKGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLFARVGRPYCFQCGEEITAQTVTQMVDAIASLPEGEKFQILAPIVRGRK
                     GEYRKELLEMRKAGYVRARVDGKIVDLGEDIALDKQKKHTIEIIVDRLVMKSGDALMR
                     RLADSVETSVKLTGGLVGVLTEDARTRLYSDKLACINCGVSYPEVTPRIFSFNSPHGA
                     CSACDGIGYAMMPGDQEEEDFTLLEPCQVCHGARLRPESLSIKLAKQSIAEVTSLSVR
                     AAADFFLSLKFTDRELVIAHRILKEIRERLGFLVNVGLDYLTLDRAAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVVVVEHDAETMQA
                     ADHILDMGPGAGSHGGHVIAQGTPQQIMGDPNSLTGQYLRGAQTVSVPQRQRKERERL
                     SVIGAQKHNLKNVTARIPLGLFTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDG
                     CKELKGVDALDKVIDIDQSPIGRTPRSNPATYTGLFGYIRDLYSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETLEILHKGKSIADV
                     LNMTVDDALEFFEHIPLIKAKLQTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSK
                     RATGRTMYILDEPTTGLHFADVQRLLDVLDRLVEAGNTVLVIEHNLDVIKNADWIIDL
                     GPEGGDRGGEIVAEGPPKDIAKVKRSYTGQVLREAGVG"
     gene            3579..3803
                     /locus_tag="VN647_10585"
     CDS             3579..3803
                     /locus_tag="VN647_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWG97519.1"
                     /translation="MNQDQFEGNWKQFKGDLKKQWGKLTDDDLLIIEGNYDKFEGRVQ
                     ERYGDKREEVKRWTDEWFKKHHPVQSEKDR"
     gene            4408..4737
                     /locus_tag="VN647_10590"
     CDS             4408..4737
                     /locus_tag="VN647_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWG97520.1"
                     /translation="MQTVALLTISNVFMMFAWYGHLKFKEYPLFTVILASWGIAFIEY
                     CFQVPANRIGHAQFSAAQLKTIQEVITLVVFSIFSVVYLKEAMKWNYVVGFALIVVAV
                     FVIFKEW"
     gene            4894..>5963
                     /locus_tag="VN647_10595"
     CDS             4894..>5963
                     /locus_tag="VN647_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWG97521.1"
                     /translation="MPKAKKSDLSAKVQSVAKKSASSPTSAPSTVEDFEKLGMFYLGR
                     PYDLATKQAKPGWLLYDSKDLVTHAVCVGMTGSGKTGLCLALLEEAAIDNIPAIIIDP
                     KGDLGNLMLTFPSLKGEDFQPWINEDDARKKGLSSADYAKAQAELWTKGLAGWQQDGA
                     RIQRLHDAADVAIYTPGSSAGLQVSILKSFAAPVADVREDAELLRERISTTVTSLLGL
                     LGIEADPIQSREHILLSTILDHTWKKEEDLDLAALIQAIQSPPVSKIGVMDMDSFFSS
                     KDRFALAMKLNNLLAAPGFQTWMEGEALDIQQLLYTSSGKPRLAIFSIAHLNDAERMF
                     FVTLLLSQMVGWMRAQSGTTSLR"
BASE COUNT         1380 a         1536 c         1674 g         1373 t
ORIGIN      
        1 ggttcccgag ttggagtcta ttctcacatt atgtataatt gtctcgtgga tatacgacca
       61 gaagtgaaag aactcatgcg cgtggccgaa agggtgattg gctttgccca tcaaaacggt
      121 ggactcaccg atgtgagtgc agtgtcatca tgtactacgc caaggaactg gaacgagaga
      181 tggcaccgtt ttgtccgaac cattgcgaac tgcccgcctc caagtgaccc atctttaaat
      241 tgaaactgac ccattaccga ctggcggtct gtctcgccgg caagcgcaaa ccgacggcgg
      301 tcggttactt gacgcggagc gaatcgccgt ggccttggcg agatcggccg cgtcgccggc
      361 tgaagaacat gaattcgtga cggcattcct gagctactgg ggtgaagcac tcgatcggac
      421 tccgcgcaga tcccgcaagc gccggtagtt atccaaagcc gcatgctata atccacctcc
      481 ccatgggcaa ctccatcatc atcaaaggtg cgcgggagca caatctcaaa aatatcgacg
      541 tggagatccc gcgcgacaag ctggtggtga tcaccggctt gagcgggtcg ggtaagtcgt
      601 cactcgcctt cgacacgatc tacgcggaag gacagcggcg gtatgtcgag tcgctgtctg
      661 cctatgcgcg gcaattcctc gagcagatgg ggaagccgga tgtcgattcg atcgaaggcc
      721 tgtctcccgc gatctccatt gagcagaaga gcacgagtca caatcctcgt tccaccgtcg
      781 gcacggtcac ggaaatctat gattatcttc ggctgctctt tgcccgcgtc ggacgtccct
      841 attgtttcca atgtggagaa gagattaccg cgcagaccgt cacgcaaatg gtcgacgcca
      901 ttgcctctct gccggaaggg gaaaagtttc agattctggc accgatcgtg cgcgggcgaa
      961 agggtgaata ccggaaggaa ctgctggaga tgcgaaaggc gggctatgtc cgcgctcggg
     1021 tcgacggcaa aatcgtcgat ctgggtgaag acattgccct tgataaacag aagaagcaca
     1081 cgatcgaaat catcgtcgat cggctggtga tgaaatccgg tgatgcactc atgcggcgac
     1141 tggcagattc cgtcgaaacg tcggttaagc tgaccggagg attggtcggg gttttaacgg
     1201 aagacgctcg aaccaggctc tacagtgaca agctggcctg tatcaattgc ggtgtgagct
     1261 atccggaagt cacccctcgg atattttcgt tcaatagccc tcacggtgcc tgttctgctt
     1321 gcgacggtat cggctatgcc atgatgccgg gtgatcaaga ggaagaggat ttcacactat
     1381 tggaaccttg tcaggtctgc catggggcaa ggctgaggcc tgaaagtctg tctattaaat
     1441 tggccaagca atcgatcgct gaggtgacca gcctttcggt tcgcgcggcg gcggattttt
     1501 ttctgtcgct gaagtttacc gatcgcgaac tggtcatcgc ccaccgaatc ttaaaagaaa
     1561 tacgcgaacg gttaggtttt ctcgtcaatg tcggcctgga ctatctgacg ctcgatcgag
     1621 ctgcggccac cttgtccggc ggcgaggggc aacgcatcag attggccacg cagatcgggt
     1681 ccggcttggt cggcgtgttg tacatcctgg acgaaccctc gatcggactt caccagcgcg
     1741 acaatcgtcg gttgttgcag acgttactca gattgcggga cctcggaaac accgtcgtcg
     1801 ttgtggagca tgatgccgag acgatgcagg ctgccgatca tattctcgat atggggccgg
     1861 gcgccggctc gcatggtggg catgtgatcg cgcaggggac gcctcaacag atcatgggcg
     1921 atcccaattc gctgacgggc caatacctgc gcggcgccca gactgtgtcc gtgccgcagc
     1981 ggcagcggaa ggagcgggaa aggctgtcgg tcattggagc gcagaagcac aatttgaaaa
     2041 atgtgactgc gcgcattccc ctggggctct tcacctgtgt gaccggggtg tccggatcag
     2101 gcaagagcac cctcgtgctg gaggtcctgt tccactctct ttcgcagctg ctctaccaca
     2161 agaaaccgaa gatcgatgga tgtaaagaat tgaaaggcgt cgatgcgctc gacaaggtga
     2221 tcgacatcga tcaatcgccg atcggccgta cgcctcggtc taatcccgcc acctatacgg
     2281 gcttgttcgg ttacatccgt gatctctatt ccaatctccc ggagtcacga gttcgtggct
     2341 ataagccagg gcgttacagt ttcaatgtga aagggggacg gtgcgaggcc tgtcaaggtg
     2401 atggactcat caagatcgag atgcatttcc taccggatgt ctacgtgacc tgcgaggtgt
     2461 gtaagggaca acgctataac cgtgaaaccc ttgagatcct ccacaaaggc aagagcatcg
     2521 ccgatgtgtt gaatatgacg gtggacgatg cgttggaatt ctttgaacat attccgctca
     2581 tcaaggcgaa acttcagacc ctgcatgatg tcggcctgca ctatgtgaag ctcggtcaat
     2641 cagcgacgac gctttccggc ggcgaagcgc aaagagtaaa gctgtcccgc gagctctcta
     2701 aacgcgcaac cggacgcacc atgtatatcc tcgatgaacc gacaacgggt ctgcatttcg
     2761 ccgatgtgca gcgattgctg gatgtgttgg atcggctggt cgaagccgga aataccgtgc
     2821 tggtgatcga acacaatctg gatgtcatca agaatgccga ttggatcatc gacctggggc
     2881 cagaaggggg cgatcgcgga ggcgagattg tggccgaagg accgccgaag gacattgcga
     2941 aggtcaagcg gtcgtacacg gggcaagtgt taagagaggc gggcgtcggg tgaagggtgc
     3001 ggtcacggaa ggatgagcct tccaggttcg ccataatgcg accgaacttc gacagcaact
     3061 tgtccactta ccccttggct gtgtgcctta ccggactgac tgacagaacg gcacccagca
     3121 ccgttgctgc caatggatgg caatagcgct acggcgaagc cttcatgctc atcccttccg
     3181 cccgccttga ggaaatgacg agtgtgggcg ctgaatggga caatgaggac aaattgtcct
     3241 atgtggcata acctatcgat ttgacaatac ctttcaaacg tatttgacat ccaccttgga
     3301 catcctgctt ctaaactctt cgctcgcgct attttatcaa tcctgagaga aaccaatgga
     3361 tcgtaaggca tttgtctgcg tatcttgttg gcccgtccct tgcctactat ttcccatagg
     3421 actggacttc cacggaggtt tttatgaaga cgatctggtc aatttcaatt ttcatagccg
     3481 tccttatcgt cacagttctt tcaaccagtt cggctgtctc cgccctggat atcgaaagct
     3541 tcaacatgca ctcagagagc ttccccggcg ttctgatcgt gaatcaggat caattcgagg
     3601 gaaattggaa acaattcaaa ggcgacctaa agaaacaatg gggaaaatta accgacgatg
     3661 atttgctgat tattgaaggc aattacgaca agttcgaggg cagggtgcaa gaacgttacg
     3721 gtgataagag agaggaagtg aaacgatgga ccgatgaatg gtttaagaaa catcaccctg
     3781 ttcagtcaga aaaagaccga taaggaacgg atgtggggat ctgaatgcct aggatgtggt
     3841 tatctccccc cgaggaggtg ccttatgcgg acagcgttga ggaatagcat aatatgcgta
     3901 ttggcggcga gtatggcagg catactcact gcctgccata ttacaaaagc gacgatcgac
     3961 acgattatcc ggtttttctc gcgcactagt ccggattcta attcagctct caaccgagac
     4021 gaccgcactc atctggagag attcgcgcgc cggaccgcgt cttgagtcga aaagcattgc
     4081 cgttcaggag aaacgcagga atttaaaagg aggatgtatg ggtacgatgg gaaaaggttt
     4141 cattttgtgg ctccttggtg ttcccgtgag cgtgttggtc ttactatggt ttctgggaat
     4201 tcttcgataa ggtgactcgc gggaggcagc cgagcggctc ctcccccgac gcactcttcc
     4261 atctgaacca tgtctttcca ttttgtggca gcgtccagcc tcacacctcc atgcagtttt
     4321 cccggtaatt gatcatttgc tttcctgctg cgcgaattca agggacaata tgtcgacatt
     4381 gcccaaaaga aaaaggagtg tgccctgatg cagactgttg cgttgctgac gatttcaaac
     4441 gtgtttatga tgttcgcatg gtacggacat ttgaaattca aagaatatcc cttgttcacg
     4501 gtcattctgg cgagttgggg cattgccttt atcgaatatt gcttccaagt gccggccaat
     4561 cggatcggtc acgcacagtt cagtgcagcc cagctcaaga cgatccagga ggtcatcacg
     4621 ctggtcgtgt tttcaatttt ctcggttgtc tatttaaaag aagcgatgaa gtggaattat
     4681 gtggtggggt tcgcacttat tgtggtggcg gtgttcgtca tcttcaaaga atggtgatgg
     4741 tcgtcggagt taagaacatc ccgtctctcg cccacaatcc atgggctcat agtcattccg
     4801 gttgatcgtt ccgcgcccgc ttgaagctca tcctgtagac cactacaata ccgcccaatt
     4861 catcccgccc gctgagcgaa gggagactgt gttatgccga aggcgaagaa gtccgacttg
     4921 agtgcgaaag tccagagtgt cgcgaagaaa tccgcctcgt cacccacctc cgccccatct
     4981 accgtagaag atttcgaaaa actcggcatg ttttatctgg gacgtcccta tgatctcgcc
     5041 acgaagcagg ccaagccggg atggcttctg tatgactcga aagatctggt gacacatgcc
     5101 gtctgcgtcg gcatgaccgg cagcggcaag accggtcttt gcctcgccct tctcgaagag
     5161 gcggcgatcg acaatattcc agccattatc atcgatccga agggcgatct cggcaatttg
     5221 atgctgacgt tcccatctct caagggggaa gactttcaac cctggatcaa tgaggacgat
     5281 gcgcgtaaga aaggcttatc atcagcagac tacgccaagg cacaggctga attgtggacc
     5341 aagggcttgg ctggctggca acaggacggt gccaggattc aacgactgca tgatgcagcg
     5401 gatgtcgcca tatacacgcc gggcagcagc gccgggttgc aggtttccat tcttaaatcg
     5461 ttcgccgcac cggttgccga tgtgcgtgag gatgcagagc ttctgcgtga acggatcagt
     5521 acgaccgtca cgagtctgct gggtctgctc ggcatcgagg ctgatccgat ccaaagtcgc
     5581 gagcatattc tcctgtccac cattctcgat cacacctgga aaaaagaaga ggaccttgat
     5641 ctcgcggcat tgatccaggc tatccaatcg ccgccggtct cgaagatcgg cgtgatggac
     5701 atggattcct tcttttcctc gaaagatcgc ttcgcactgg ccatgaagtt gaacaatctg
     5761 cttgccgccc caggatttca aacctggatg gaaggagaag cactcgatat tcagcaactg
     5821 ctgtatacct cgtcgggcaa gccgcggctg gcgatttttt ccatcgcgca tttgaatgat
     5881 gccgaacgca tgttcttcgt gacgttgttg ctcagtcaga tggtcgggtg gatgagagcg
     5941 cagtcgggta ccaccagcct gcg
//
LOCUS       DATNDD010000438         3792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_210111, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000438 DATNDD010000000
VERSION     DATNDD010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210111"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VN647_10600"
     CDS             <1..571
                     /locus_tag="VN647_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97522.1"
                     /translation="YKLKEVQVTLPNGVLITAEISTIEDVKELLKALEQLDLGLSDRT
                     LDEAHKHTEKKTTHSDSDMPEVRVETRADLIRGSLRAKNVLAFKDSVPQLLRPNAFSS
                     ATDASLALLFSVEAGLKKNNLEFDAFKGLYESQNIKSGSPISMLLTNLRNAQYLDSKL
                     YSSERKLRLTAKGETKAIEVLKALVEKSK"
     gene            1029..1271
                     /locus_tag="VN647_10605"
     CDS             1029..1271
                     /locus_tag="VN647_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWG97523.1"
                     /translation="MPRSKIAISLDQSTLERLDTLVEQAAFPSRSQAIQEAVEEKLAR
                     LDRSRLAKECAKLDPVFERTLAEEGLSEDVSAWPEY"
     gene            1256..1594
                     /locus_tag="VN647_10610"
     CDS             1256..1594
                     /locus_tag="VN647_10610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWG97524.1"
                     /translation="MARILRGEIRWADLNPVRGKEQAGRRPVLVLSHDVFNERSGTVI
                     AIALTSQPQRAGFPLTLEVQSKGLPKKSWVKISQIRTLAVERIGQRLGKVSLEDLAQV
                     IEGLNEIIGG"
     gene            1734..1898
                     /locus_tag="VN647_10615"
     CDS             1734..1898
                     /locus_tag="VN647_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97525.1"
                     /translation="MKRKGSISSDDLHPEYTSTIRRRFVENIIGLIREGTNVVVFDPD
                     VAKAFPIRQQ"
     gene            2618..2833
                     /locus_tag="VN647_10620"
     CDS             2618..2833
                     /locus_tag="VN647_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97526.1"
                     /translation="MKKQPRKKLIHEGQYLAEVDIELVVTDDEWSPYLSVEDVYKLDD
                     VREALKKGDTTTAARYGRVFSLTPIAV"
     gene            2859..3119
                     /locus_tag="VN647_10625"
     CDS             2859..3119
                     /locus_tag="VN647_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97527.1"
                     /translation="MKLITRKTVAEKLSAHLRHDLSLDSLVWAESAMMDGEFDPAHLP
                     TIRNVVARIGVADVRAFGLTWDDCEQLLSQLGYSAQVSIVDR"
     gene            3307..>3792
                     /locus_tag="VN647_10630"
     CDS             3307..>3792
                     /locus_tag="VN647_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97528.1"
                     /translation="MNPTNLGNQLNAIVNAAKGATFDQGELAQLTYGAFDVAAQKVRE
                     LEQDEIEIAFPVGYKPDRTPIPGSSKYRKDQLLQRYQFLAFHQLTVNALVQLVTIVET
                     MLGDVLRAVVLRYPQKLGGKRSILIQTVLESASLEEVHLRATDALLNELGYKSPADFA
                     ES"
BASE COUNT          999 a          969 c         1004 g          820 t
ORIGIN      
        1 ttacaagctt aaggaggttc aggttactct tcccaatggt gttcttatta cggctgagat
       61 cagcactatt gaggatgtga aggagctact taaagcgctt gagcagctcg atcttggttt
      121 atcagatcga actctagatg aggcacataa gcacacagag aagaagacaa cacattcaga
      181 ctccgacatg cccgaagtgc gtgttgaaac ccgagcagat ctaatcagag gaagccttcg
      241 tgccaagaac gtattggctt ttaaggatag cgtcccccaa ttgctacgac cgaatgcctt
      301 tagttcggct actgatgctt cacttgcatt gttattttcc gtagaggcgg ggttgaagaa
      361 aaataacttg gagtttgatg cattcaaagg gctttacgag tcgcagaata taaagagtgg
      421 aagtcccatc tccatgttgc tcacaaatct cagaaatgct cagtatctcg actcaaaact
      481 gtactcgtcg gaaagaaagt tgcgtctgac cgccaaggga gagacaaagg caattgaagt
      541 cttgaaagca ttagtggaga agtccaagta gtagcgcgca catccaccga gcaccgcctg
      601 atttaatgta agtctgatga gtcttgcgtg cgcagtgggc gagcacagag gatcagccgc
      661 gctgcccgat cttgctctcc atccccttcc acaaccgctc aatattcccc ttgtgcttga
      721 tcacgatagc gcagtcacca gcacagagaa caaagcaaag agagccgtcg gtcgaagcga
      781 tgcagcaagg atgggaaaga gtccaaatgc ggtgagtgcg ccgtggaaga gtagcgccag
      841 agggccacgg ctttagaaac aattggtggt cagagtgtca ttgtttgcgg acgcccgaat
      901 cacggaagcc attctctcgc aattgctttc tgactacgat atccagaccc ggtgcgttag
      961 gaccccgatg cgagaatgag cttgacccag acttaggtat gacatattgt cataccttga
     1021 ggtgcaacat gccgcggtca aaaatagcga tatccctcga tcagtccacg ttagaacgcc
     1081 tcgataccct cgtcgagcaa gccgcctttc ccagtcggag tcaagccatt caagaggcag
     1141 tagaggaaaa acttgcccgc ttggatcgaa gccgcttggc caaggagtgt gcgaaactcg
     1201 atcccgtctt cgaaaggacg cttgccgagg aaggcctgtc ggaggatgtt tcagcatggc
     1261 ccgaatactg aggggtgaga ttcggtgggc ggatctcaac ccggtacgcg gcaaagaaca
     1321 agccggacgg cgtcccgtgc tggttctcag ccacgacgta ttcaatgaac gttccggcac
     1381 ggtgatcgcg attgcgctca cgagccaacc acaacgagcg ggtttccctc tcacacttga
     1441 ggttcagtcg aaaggtcttc cgaagaaatc atgggtaaag atcagtcaga ttcgcacgct
     1501 ggcggtcgaa cggatcggtc agcgactggg caaggtgagc ttggaagacc tggctcaggt
     1561 gatcgaaggt ctgaatgaaa tcatcggcgg ctgatcaggc aggggcaaaa gcacatgaga
     1621 cagagctccg tgcaaactgg agaagactgc gaacacttcc tcgcgcaact cggccactcc
     1681 ggccatacgc atcatcagcg cacgacctgc gacgcgacac gagaggaagc gacatgaaac
     1741 gtaaaggctc aatctccagc gacgatctcc accccgaata cacttcgact attcgaaggc
     1801 ggttcgtgga aaatattatc ggcctcataa gggaaggaac gaatgtcgtc gtctttgatc
     1861 ccgatgtcgc caaagctttt ccaattcgac agcagtgaac gatgccttgt ggctgaatcc
     1921 gccctgatac agttcgcagc cattcgcgat acaacagccg cataggcctc gccgacgtac
     1981 gcgccttcgg tctcacctgg gaagactgcg aacaactcct cacccaactc ggctattccg
     2041 cacaagtcaa catcgtcgcc cggttgtggg tgcctattcg ggatgcttat acagacccgc
     2101 aaaaaccgca taagcgcgta gtgtaggaat agaatagtga agaaacctct tggacttagg
     2161 acccaatcct ggcaggatgt ggtacggaac ttatgcatcc cacaggacac ctagcttctg
     2221 atgcaaacca gatgatgcat ggttaaacag ggagaaacag cagaaggacc gatgatcgtg
     2281 aaggtcggcg ggtaaccgtg agtgatacgt tcgagcgcgt caaacgtctc gtcgcaggta
     2341 gatccgttcg gatttctgag cacgattacg atgagcttgt agcagatgat attcgctccc
     2401 gtgatattat tgaacacatc gacgatggac gagtagtaga agattatccg gattatccca
     2461 aaggggcctg tgttttggtg ctccagagag atagaaatga aaatccgatt cacgtcttat
     2521 ggggtattcc caaaggacat tcagaaccgg ccgttgtggt tactgcctat cgacccgatc
     2581 ctgacctatg gcaggaagga ttcatgagga gacgtacatg aaaaaacaac cacgaaagaa
     2641 gctgattcat gagggacagt atctggctga agtcgatatt gagctagtgg tgactgatga
     2701 cgaatggtct ccctacctct cggtggaaga cgtctacaag ctcgatgatg tgcgcgaagc
     2761 gctgaaaaag ggagacacca cgacagcagc acgatacggt cgtgtgtttt ctctgactcc
     2821 catcgcagtc taaccattgc tacccgaaga tataaaacat gaagctgatc acgcgtaaga
     2881 ccgttgctga aaagctttcc gcccatctgc gacatgactt gtcattggac agcctagtat
     2941 gggcagaatc ggcaatgatg gacggtgaat ttgatccagc tcaccttccg accattcgca
     3001 acgtcgtggc ccggatcggc gtcgccgacg tacgcgcatt cggcctcacc tgggacgact
     3061 gcgaacaact tttgtctcag ctcggctact ccgcacaagt cagcatcgtt gaccgatagt
     3121 ctccctacaa cacgctctga catactcgga tcggctccac caccagaacg agtatgattc
     3181 cctcgcgcag gaccatggag agcgttgaca agtaaggtaa cctgcgcaag aatcgctcgc
     3241 cgattttcga tctgtcggtg tgtactatgc cggtcagcat acgcaatagt taggcatttc
     3301 gttcacatga accccacgaa tctaggcaat cagttgaatg caatcgtcaa tgccgcaaag
     3361 ggcgcgacgt ttgaccaagg cgaactagca cagttaacgt atggcgcctt tgacgtcgct
     3421 gcgcagaaag tgcgggaact cgaacaggat gagattgaga ttgcgtttcc agttggctac
     3481 aaaccggaca gaacgcccat tccaggctct agcaagtatc gaaaggatca actgcttcaa
     3541 aggtatcaat ttctcgcatt tcaccaactg acagtgaacg cactcgtgca gctcgtgacg
     3601 atcgtcgaga ccatgcttgg cgatgtactg cgcgcggttg tactgcgata tccgcagaaa
     3661 ttgggcggga aacgtagcat cctaatccaa accgtacttg agtccgcttc attggaagag
     3721 gtgcacttgc gggctacaga tgctttgctc aatgagctcg gctacaagtc cccggccgac
     3781 ttcgctgagt cg
//
LOCUS       DATNDD010000439         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2107616, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000439 DATNDD010000000
VERSION     DATNDD010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107616"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..925)
                     /gene="pssA"
                     /locus_tag="VN647_10635"
     CDS             complement(119..925)
                     /gene="pssA"
                     /locus_tag="VN647_10635"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWG97529.1"
                     /translation="MKTAGFRSSFGKEGKRRKAAMHLIPNLFTTGNLFCGVYAILSVF
                     NANYVEAAIAILVAMIFDVLDGKSARLTNSTSQFGLEYDSLSDVVSFGVAPGLLIYSW
                     ALSGQGTFGVAVMFAYVAMGAVRLARFNSTVALSDGKYFTGLAIPAAAGVVASLVVFD
                     HYLLKMGGDVRPIVVLLMTLALSFLMVSTIKYRSFKELKFKGHRQITYLVWGILTLMM
                     VAAWPAVMLFVIFAGYALMGPVEKVFWVVAPAAGKKSIGKVESPPIESNS"
     gene            complement(922..1572)
                     /locus_tag="VN647_10640"
     CDS             complement(922..1572)
                     /locus_tag="VN647_10640"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HWG97530.1"
                     /translation="MADQAVGVPFAKEGIPFIAVPGSVTLLTAVLGWPVVACVGAIAT
                     LFSAWFFRNPARVVPQGPKLVVAPGDGKVIAIEEEFEPRYLKERSVRLTIFLNVFDVH
                     INRMPCDGVIEDVQYQPGLFLVASKPEATLRNEQNTMMIKTHEGIKVLCVQVAGLIAR
                     RIVCWISPRDRAIRGERYGLIRFGSRMDTFLPMGTILRVAVGERVKGGETILGELP"
     gene            complement(1582..2595)
                     /gene="ilvC"
                     /locus_tag="VN647_10645"
     CDS             complement(1582..2595)
                     /gene="ilvC"
                     /locus_tag="VN647_10645"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWG97531.1"
                     /translation="MKIYYDKDADIQQIRNKTVAVIGYGSQGHAHALNLKESGVTVVI
                     GLREGASWKKAEQSGLKVMPVVDAVKASDVVMILAPDEAQAAIYRQGVAPNLKAGSYL
                     AFGHGFNIHFGQIVPPASINVFMVAPKGPGHLVRSEYTKGSGVPCLLAIHQDPSGTAR
                     QVGLAYASAIGGGRAGVIETNFREETETDLFGEQAVLCGGLTSLIQAGYETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRVVTEQTKQEMKRI
                     LEEIQTGRFAKEWVLENQANRPVYNALLAKGEAHPIEAVGAKLRGMMPWLKKDQLVDK
                     TKN"
     gene            complement(2651..3169)
                     /gene="ilvN"
                     /locus_tag="VN647_10650"
     CDS             complement(2651..3169)
                     /gene="ilvN"
                     /locus_tag="VN647_10650"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWG97532.1"
                     /translation="MEHIISVTVENKFGVLSRVAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIIEQIVKQLNKLIDVIKVVDLNEAEFVSRETAIIKVHTKDADRAEAL
                     RIVDIFRANVIDSTPSTYTIEVSGDPKKIEAIINLLQPLGIKELVRTGRVAVAREPVR
                     PVAIQAKKVARD"
BASE COUNT          770 a         1053 c          776 g          757 t
ORIGIN      
        1 tttgcaccac ccaccagctc tcttcagagt gttcaccacc ctatttctgg acggctcgcg
       61 tactactcct cgtcttaacc cttcaaaacc ttttcagtcc tcccacggtt attcaccgtt
      121 acgagtttga ctcgatcggc ggagactcaa ccttcccgat gctctttttc ccggctgccg
      181 gagcaaccac ccaaaagact ttttcgaccg gccccatcaa tgcatagccg gcaaagatga
      241 caaataacat aaccgccggc caggctgcta ccatcatcag cgtaagaata ccccagacca
      301 gataggtaat ctgccggtgg cctttgaatt tcagttcttt aaaactccga tacttaatcg
      361 tgctgaccat taaaaacgaa agcgccaaag tcataagcaa taccacgatc ggtctgacgt
      421 cccctcccat cttgaggagg taatgatcaa agaccaccag ggacgccacc accccggcag
      481 cggctggaat agccagaccg gtaaagtact tcccgtccga cagcgccacg gtcgagttga
      541 atcgcgccaa tcgcacggct cccatggcca cataggcaaa catcacggcc acgccgaacg
      601 taccctgacc gctcaacgcc caggagtaga tcaacaaacc tggtgcgact ccgaatgaca
      661 ccacatcgga cagcgaatca tactccagcc caaactgact cgtactgttg gtcagtctgg
      721 ccgatttccc gtccaagacg tcaaaaatca tcgcgacaag aatcgcgatg gccgcttcca
      781 catagttggc gttgaagacg gacaagatcg catagactcc gcaaaataag ttgccggtcg
      841 tgaagaggtt cggaataaga tgcatggcgg ccttccgcct cttcccttcc tttccaaatg
      901 aacttctgaa acccgccgtt ttcatggcaa ctctcccaga atggtttcac cacccttcac
      961 ccgctcaccc acggccactc ggagtatcgt gcccatcggt agaaaggtat ccatgcgcga
     1021 tccgaaacgg atcaatccat atcgctcacc tcgaatggct cgatcccgtg gcgagatcca
     1081 acacacgatg cgtcgggcga tcaaccctgc tacttgcacg cacagcactt tgatgccttc
     1141 gtgcgtcttg atcatcatgg tgttctgttc gttccgcaac gtcgcttccg gcttgctggc
     1201 gaccaaaaac agccccggtt gatattgcac atcttcgatc actccatcac agggcatcct
     1261 attgatgtgc acatcgaaga cattcaaaaa aatcgtaagc ctgacactgc gctctttgag
     1321 gtagcgcggc tcaaactctt cctcgatggc gatcactttc ccatcgccgg gagcgaccac
     1381 aagctttggg ccttgcggca cgacccgagc cggattccga aaaaaccaag cagaaaacaa
     1441 cgtcgcgata gcgccgacac atgcgacgac cggccagccc agcactgcag tcaacagtgt
     1501 aacgctgcct ggaaccgcga tgaagggaat tccttcttta gcaaagggaa cgccgaccgc
     1561 ttgatccgcc acggagttgc ctcagttttt agttttgtcg acaagctgat ccttcttgag
     1621 ccacggcatc atcccgcgca gcttggctcc taccgcctcg ataggatgcg cctcgccctt
     1681 agcaagcaat gcattataga ccggtcgatt ggcttgattc tccaagaccc attctttggc
     1741 aaaccgtccc gtctgaatct cttcgagaat ccgcttcatc tcttgcttcg tctgttcggt
     1801 caccacacgc ggacctctgg tcacatcacc gtattttgcc gtcgtgctga tcgaatagcg
     1861 catgttggcg atcccaccct gataaatcag atcgacgatg agcttcacct catgcaaaca
     1921 ttcgaagtag gccatctccg gcgagtaccc ggcttcgacc aaggtctcat accctgcctg
     1981 gatcagggac gtcagccctc cgcagagcac ggcctgctcg ccgaagagat cggtctcggt
     2041 ttcttcgcga aaattcgtct cgatgacacc ggctcgtcca ccgccgatcg cactcgcgta
     2101 ggccaatccg acctgcctcg cggtgccgct gggatcctga tggatcgcca ggagacaagg
     2161 caccccgcta cctttcgtat actcggaacg aacgagatgt cccggcccct taggggctac
     2221 catgaagaca ttgatggaag ccggcggcac aatctgccca aagtgaatgt tgaaaccatg
     2281 accgaatgcc aaataggatc cagccttaag attgggcgcc actccctgcc gatagatggc
     2341 ggcttgcgct tcatcgggtg ccagaatcat cacgacatcg gaagccttca cggcatcaac
     2401 gacgggcatg accttgagtc cgctctgttc ggcttttttc cacgaggcgc cctcacgcaa
     2461 cccaatgacg accgtcacac cgctttcttt caggttgagc gcatgcgcat ggccctgact
     2521 cccatagccg atcacggcca cggtcttgtt tcgaatctgc tggatatcgg catccttatc
     2581 atagtaaatt ttcatgacca accctttcat ctcatcagtt atggagcaaa actgtgcgcg
     2641 tcgcaggatg ttagtcgcgg gcgaccttct ttgcttgaat cgcaacagga cgaaccggtt
     2701 cccgtgcgac ggccacccga ccggtgcgga ccaactcttt gataccgagc ggctgaagca
     2761 agttgatgat cgcttcaatt ttcttaggat ctcccgagac ttcgatggtg taggtgctcg
     2821 gtgtcgaatc gatcacgttc gcccgaaaaa tatcgacgat tctgagcgcc tcggctcgat
     2881 ccgcatcctt cgtgtgcacc ttgatgatcg ccgtctctcg tgaaacaaac tccgcttcgt
     2941 tcaagtccac gaccttgatg acgtcgatga gtttattcag ctgcttcacg atctgctcga
     3001 tgattcgttc atcaccagac gtcacgatcg tcatctgaga catggatgga tccaatgtcg
     3061 gagcgaccga caggctctca atattgaacc cgcggccgct gaataacccc gcgacccgtg
     3121 acaaaacccc aaacttattt tctaccgtga ccgagataat atgttccatc ttcctcagtg
     3181 ctgagacgtg agtgctgagg ccaagcccga ccactcagca ctgagcactt gtcgctcagc
     3241 acttcctttt atgccgtgag gaccgtatcc ttatcctccg gcgtcaccgt tgccgcaccg
     3301 ggttgtttct tcttcaactc aggcggatcc tccaggatca tctcatggtt gcagcc
//
LOCUS       DATNDD010000440         5196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4221586, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000440 DATNDD010000000
VERSION     DATNDD010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221586"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..882
                     /locus_tag="VN647_10655"
     CDS             538..882
                     /locus_tag="VN647_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWG97533.1"
                     /translation="MDNMTPLPEFLVRLDEGVIRVAGTRVSLDSVIRAFQDGATPEEM
                     CLDFPSLDLAKVYSVLAYYLTRRETIDVYLLAQQRLSETIRQEFQARHSPFLGELRRR
                     VAARRALPPACA"
     gene            873..1235
                     /locus_tag="VN647_10660"
     CDS             873..1235
                     /locus_tag="VN647_10660"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWG97534.1"
                     /translation="MRVSVVRFLFDEDCNGRIVRAVRRRTPTLNTVTALEAGLGEATD
                     DAVLECACTESRVVISHDHSTMRARAEDRLRAGLPMAGLILVPQDVPVGKVIEELVLI
                     AETTSPEEWQGQIVFLPL"
     gene            complement(1308..3155)
                     /locus_tag="VN647_10665"
     CDS             complement(1308..3155)
                     /locus_tag="VN647_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG97535.1"
                     /translation="MRNTRMVLYDRSLRQKGMLLVRTLLISLFLSGAIVACAASPQTK
                     TTLSPPATAVQPPPSAPESDATYHFMMGHQAELAQDLDAALKEYHTALKVDPKSREVK
                     SRLAALYFAMGDVTQAVQYADEVGEGTGQEPQMLTHMAGILASAGKQERALELLDKAI
                     ESDPERGESYFPKGLILLNQKRVAEAEQVVKKGLQYTSDSPIGYYYLGRISMEAGNPE
                     QAVANFDRAIAVNQAFEPAYLAQASVHESRQEKGKAIAVLQKYLHEVNPRNRDIRQHL
                     VQLYIATKDYAGGLAELEKLLEDNPGDLDAQLRMALIYGEKKEFTKAINQLTEILKAK
                     PAELKVRDYLGYLYEETKDFPKAMETYRYNIHLDPKFPDSHVHLGVLLYRLKQFPEAV
                     THLDEAVRLTPKQPEPYIVLGLAHFQNEQFEKASQTFEEGIRHNPKSADLHFNLGTAY
                     DKLNRFDQVERAMETALSLDPHHADALNYLGYSYAERGIKIDQALSLTKRAVALKPEN
                     GYYVDSLGWAFYKSGMLTEALTEIKKAVALVGDDPVIYEHLGEIYLKQQKVADAREAW
                     FHSLEIDPSNEKLLQRFREQGLANPTYEDRIQQAKRRVSEKAQTQQAIP"
     gene            complement(3503..4855)
                     /gene="dnaB"
                     /locus_tag="VN647_10670"
     CDS             complement(3503..4855)
                     /gene="dnaB"
                     /locus_tag="VN647_10670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWG97536.1"
                     /translation="MKSTGTVDLSQPKLPPQNLEAEQSVLGAILLDNAAMPKAMELLV
                     EEDFYRTAHKKVYQAMLELSDTGEVIDQITLTERLKSRGELEAIGGAAYLAELVQIVP
                     SSANIRYHCKIVRDKAVARQLISTSTEVLTRGYEGTASIDDLLDFAERSVFGIAQGKL
                     ERSFSPISQVIKESLDVIDKLSKRKEHVTGVPTGYYDLDDITAGLQASDLVVVAGRPS
                     MGKTSLALGFATHAAIHANTVVGVFSLEMSKPQIVLRMLSSEARVDSHALRTGKLQKE
                     DWWRLAEAAGRLEQAPIYIDDTGGITVQQMRGKARRLKAEKGLDLLIVDYLQLMQGRS
                     DSESRQQEISDISRSLKALAKELNVPVVALSQLSRAVEARKPPIPMLADLRESGAIEQ
                     DADVVIFIYREDVYDSNSERKGIADIIVSKHRNGPIGKKELFFQDRFAKFESLDLREA
                     "
     gene            complement(4852..>5196)
                     /locus_tag="VN647_10675"
     CDS             complement(4852..>5196)
                     /locus_tag="VN647_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97537.1"
                     /translation="VGPEVLVVAPLGLSTRVVSVAQQLRRAGIRTVQQVVDPSGRGLI
                     GAAAKAGLEAEIDTIIVIGADSPASEQVVVLQRGDRPRVGGSTGRAYVRKRTMSIAGL
                     IASLRADLEGAV"
BASE COUNT         1075 a         1455 c         1429 g         1237 t
ORIGIN      
        1 atcgatgtct tgatggccat caagttttcc ttagcctccg attgccgcga cttcatctga
       61 tactgcaaga agttgggaat ggcgatggcc gccaggatcc cgatgatcgc cacgacgatc
      121 atcaactcaa tgagggtgaa cccctcttgt ttacggagcg acttcaacat cgtattgcct
      181 ccttgttgat ggttcactga cggccccctt atcatccgcg gcgctcatgt tgccgcctac
      241 cggtccctat agcaggtttg gtgccgatca atgacaaggc ctagcctagc attgatccct
      301 gacgatgcaa caacttacga gaaatcgacg ggtgatggag ttaggaaaga agctaggtgc
      361 tcttccgtaa tctgctccgg agagccaaga attgacactt tttgccctgt ccaaccggta
      421 ttcaggcgcg agtatcggaa gactgtcagg taaggcaata gggagaacgg tcggtgatgg
      481 aagtagattt ttcagccgag gttgcgattc caattcggca ggtttacgat gtgccctatg
      541 gataacatga cacccctccc ggagttcctt gttcgcctgg acgaaggcgt cattcgagtg
      601 gccggaactc gtgtgtctct cgacagtgtg attcgggcct ttcaggacgg cgccacgccc
      661 gaagaaatgt gcctcgattt tccctccttg gatctcgcca aggtctacag cgttttggca
      721 tactacctca cgcgtcgcga aaccatcgac gtgtatctgc tggcgcagca gcggttgtct
      781 gagacgattc ggcaggaatt ccaggcgcgt cacagcccat ttcttggtga gttgagacgg
      841 cgtgtcgctg ctaggcgcgc gctccctccg gcatgcgcgt gagcgttgtt cgttttctct
      901 tcgatgaaga ttgcaatggg aggattgtcc gcgccgtgcg gagacggacg ccgacgctca
      961 ataccgtcac ggctctggaa gcgggactcg gtgaagcgac ggacgacgcg gtactggaat
     1021 gcgcctgcac cgagagccgc gtggtgatct ctcacgatca cagcacaatg agggctcgcg
     1081 cggaggacag actacgagca ggactgccga tggccggatt gatcctggtc ccgcaagatg
     1141 tgccggtggg aaaagttatt gaggaattgg tcctgatcgc agagacgacc tcaccagagg
     1201 aatggcaagg acaaatcgtg tttcttccgc tataagtctg accgaaaccc tgtcacgctc
     1261 gtcatgattt tgtcggagcg ggaggaatcc attctagagc cgtggtttta aggtatcgcc
     1321 tgttgagtct gtgctttctc cgacacccgg cgcttggcct gttggatacg gtcttcgtag
     1381 gtcgggttag ccaggccctg ctcccggaaa cgttggagca gcttttcatt ggaagggtca
     1441 atctccagtg agtggaacca ggcttcacga gcatcggcaa ctttctgctg cttcaaatag
     1501 atttccccca aatgctcgta gatgacgggg tcatcgccga ccaatgccac ggctttcttg
     1561 atctccgtga gcgcctcggt cagcatgcct gatttgtaaa aggcccaccc gagactatcg
     1621 acatagtagc cgttctccgg cttgagtgcg accgcccgtt tcgtcaggga caaggcttga
     1681 tcgatcttga tgccgcgctc cgcataactg tagccgagat agttcaaggc atcggcgtga
     1741 tgagggtcga gggagagcgc ggtttccatg gcgcgctcca cttggtcaaa ccgattcagt
     1801 ttgtcgtagg ccgtgccgag gttgaaatgc agatcggcgc tcttcggatt gtgccgaatc
     1861 ccctcctcaa acgtctgtga cgccttttcg aattgttcgt tctggaaatg cgccagccct
     1921 aggacgatat atggttcagg ctgtttcggc gtgaggcgaa cagcttcatc gagatgggtc
     1981 accgcctccg ggaattgctt gagtcgatag aggagaacgc ccagatgaac atggctgtcg
     2041 ggaaactttg ggtccagatg aatattatag cgataggttt ccatcgcctt tgggaagtcc
     2101 ttggtttctt catacaggta ccccaggtaa tctctcactt taagttcagc cggcttggcc
     2161 ttcaaaattt ccgtcaattg gttgatcgcc tttgtaaact ccttcttctc tccataaatc
     2221 agcgccatgc gtaactgcgc atcgagatcg ccgggattgt cttccaacaa cttctccaac
     2281 tccgcaaggc cgccggcata gtctttggtc gcgatataca actgaacgag atgttgtcga
     2341 atgtccctgt tccgaggatt cacctcgtgg agatactttt gaaggacggc gatggccttg
     2401 cccttctctt ggcgtgattc gtggactgac gcttgcgcca aataagcagg ctcgaacgcc
     2461 tggttcacgg cgatggcccg atcgaaattg gccacagcct gttcaggatt tcctgcttcc
     2521 atcgaaatgc ggcccaaata atagtagccg atcggcgaat cagaggtata ctgcaatcct
     2581 ttcttgacga cctgttccgc ttcggcgact cgtttttggt tcaggagaat gagtcccttc
     2641 ggaaaatagg attctcctct ttcgggatcg ctctcgatgg ccttgtccaa taactccaag
     2701 gcgcgctcct gcttccccgc actggctaag atgccggcca tatgggtcag catttgcggt
     2761 tcttgccccg ttccttctcc gacttcatcc gcatactgca cggcctgagt cacatctccc
     2821 atcgcgaaat acagagcggc caaccgagat ttgacctcgc gggatttcgg gtcgactttc
     2881 aaagcggtat gatattcctt caatgcggca tccaggtcct gagcgagttc cgcttgatgt
     2941 cccatcataa aatggtaggt ggcatccgac tcgggagccg acggcggagg ctgcactgct
     3001 gtggcaggag gagaaagggt tgtcttggtc tggggggagg ccgcgcaggc gactatggcg
     3061 ccggagagga acagggagat cagcagcgtc ctcactaaga gcataccctt ctgtctaagg
     3121 gaccgatcgt acaagaccat tcgtgtgttc ctcataatac cgttcgtcag gacaagtggt
     3181 ggcgggccaa tgaaaggatt ataccgagtg tcggggcgat gcgcaaacga ctctatcagg
     3241 atgttgaaaa agtcctccag cttcgttctc gcatcgctca aggtctcaac gtaccaccgg
     3301 gcaagagctg ccaaagcagc tcggggcggg tgggtgagaa aaaattacgc ctcggcctct
     3361 gggacactgg gcgctcaccg actcgtgtcc gtccgcaaac gtgacgctca ttcttcttcg
     3421 cgtcgcggac ctcgctgcgg ccttgctgga aaggcctttt tgaacatcct gcggtgatgc
     3481 tgagatattt atcaacagtt gactatgctt cgcgaagatc gaggctttcg aatttggcga
     3541 atcggtcctg gaaaaacaac tcctttttcc caataggacc attccgatgt ttactgacga
     3601 tgatgtcggc aattcctttg cgctccgaat tcgagtcgta cacgtcttct cggtaaatga
     3661 agatgaccac atcggcatcc tgttcgatcg ctccgctctc ccgcaaatcg gccagcatgg
     3721 ggatcggcgg cttacgggcc tctaccgcac gactcaactg agatagcgct acgaccggca
     3781 cattcagctc ttttgccaat gcctttaacg agcgggaaat atcggagatt tcttgctgac
     3841 gggactccga atcgcttcgc ccttgcatga gctggaggta atccacgata agaagatcaa
     3901 gtcctttctc cgctttgagc cggcgggctt tgcctcgcat ctgttggacg gtaatgccgc
     3961 ctgtgtcgtc gatatagatc ggtgcttgtt ccaatcggcc ggccgcctcc gccaatcgcc
     4021 accaatcctc cttttgaagc ttccccgtcc gcagcgcatg ggaatcgact cgcgcctcag
     4081 agctgagcat acgaaggacg atctgcggtt tggacatttc caagctgaag actcccacca
     4141 ccgtattggc atgaatggcg gcatgcgtcg caaaccccaa ggcgaggctc gtcttaccca
     4201 tgctcggccg cccggccacc accaccaagt cggaggcctg aagccctgcg gtaatatcgt
     4261 cgaggtcgta gtatcccgtg ggcactcctg tgacgtgttc ctttcgcttc gaaagtttat
     4321 cgatgacatc cagactttcc ttgatgacct gactgatcgg gctgaacgac cgttccagtt
     4381 tgccttgcgc aatgccgaag accgaccgtt cggcaaaatc gagcaggtcg tcgattgagg
     4441 cggtgccttc atagcccctg gtcagcacct cggtggaggt gctgatcaac tgacgggcca
     4501 ccgccttgtc ccgaacgatc ttgcagtgat accgaatatt ggcggaactg ggaacgatct
     4561 ggacgagttc ggcgagatag gccgcacctc cgatagcctc aagttcaccg cgagatttca
     4621 atcgttcggt cagcgtgatc tgatcgatca cttctccggt gtcggacagc tcaagcatgg
     4681 cttgatagac ttttttgtgc gccgtccgat aaaaatcttc ctccaccagc agttccatcg
     4741 ccttcggcat ggcggcgtta tcgaggagaa tggcgccgag caccgactgc tccgcttcca
     4801 agttctgcgg agggagtttc ggttgagaaa gatccactgt cccggtggat ttcataccgc
     4861 tccttcgagg tcggcacgta gacttgcgat gagaccggcg atcgacatgg tccttttgcg
     4921 aacatatgca cggcctgtcg atcctcccac tcgcgggcgg tcgccccgct gaagcacgac
     4981 aacctgctcg gatgcgggac tgtccgcgcc gatgacaata atggtatcga tttctgcttc
     5041 aagcccggct ttggccgcgg caccgatcaa accccgtcca gatggatcca cgacctgttg
     5101 aaccgttcgt atccctgctc ggcgaagttg ttgagcgacc gaaaccactc gcgtcgacaa
     5161 ccccagcgga gccacgacca acacctccgg tcccac
//
LOCUS       DATNDD010000441         8514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3081056, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000441 DATNDD010000000
VERSION     DATNDD010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3081056"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VN647_10680"
     CDS             complement(<1..804)
                     /locus_tag="VN647_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWG97538.1"
                     /translation="MGTIRQGDSVKKSSSASLPREKSNGKSEVPSPKTLRQYLQRFPQ
                     TSVLVVGDLILDHYVMGRVSRISPEAPVPVVHVESESLRLGGAANVFNNILALGGKAD
                     LCGVIGSDESGRLLMKELGDKRSNRGGVVIDHDRPTTRKSRVIAHNQQIVRYDIEGRS
                     ELKTALRQKILRYVESRIKELSCVVVSDYAKGVVSPALMSEMTRLAASQKVPVVVDPK
                     VEHFSYYKGVTVLTPNHLEAAQASGVHGDDDQTIDEAGAMIRQRLGCRSV"
     gene            complement(774..1544)
                     /gene="lepB"
                     /locus_tag="VN647_10685"
     CDS             complement(774..1544)
                     /gene="lepB"
                     /locus_tag="VN647_10685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWG97539.1"
                     /translation="MSLDQNQRNVEKLPGSPRGGEQASLPGAKLADNMDQSGRKSIVR
                     EYAEAIIVAMLLAFAIRVFVVQAFKIPSGSMIPTLLIGDHILVSKLSYGLQWPTDCQM
                     QWTFPPVNCYTSKTVVAFGKPQRGDIIVFRFPEDEEKDFIKRIVGLPGDTVQLRNKVV
                     LVNGRALDDKAFTQRIDPGVIDGTVNPRDNFGPVTIPEGSYFVMGDNRDQSLDSRFWG
                     YVREEKIRGKAFRIYWSWNGQGNWTEWVRWERFAKAIQ"
     gene            complement(1541..3340)
                     /gene="lepA"
                     /locus_tag="VN647_10690"
     CDS             complement(1541..3340)
                     /gene="lepA"
                     /locus_tag="VN647_10690"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWG97540.1"
                     /translation="MQSLIRNFSIIAHIDHGKSTLADRFLEATGAVTAREAKDQILDA
                     MDLERERGITIKAHAVAIRYKARDGKTYALHLIDTPGHVDFTYEVSRSLAACEGALLL
                     IDATQGVQAQTIANVNLAMANNLTIIPVINKIDLASADVEGTKQSISEVLQLEAAEAL
                     PISAKEGKGVSEVLEAVIARIPPPSGDPQAPFKALIFDSWFDNYQGVIVLARLVDGSI
                     RPGMKIKVMSNDRTFEVMEVGQFTPKRTKKAELLTGEVGYLCANMREVADVKIGDTLT
                     DAVTPTAAPFPGYKEVKPLVFCGLYSTDTAKYEDLRDALVKLRLNDSSFVYEPETSLA
                     LGFGFRCGFLGLLHMEIIQERLEREYGLTLITTAPTVVYHVTTTKGDKLEIDNPAELP
                     PPNNIASFEEPFILATVITPERYMGSILQLCQERRGIQRSLQFLDPTRVMISYELPLN
                     EVILDFYDKLKSRTQGYASLDYELLGYRESDLVKIDILLNGEAVDALSFITHRERSYH
                     RGRQMAEKMKELIPRQMFEIAIQAAIGNKIVARETIGAMKKNVTAKCYGGDITRKRKL
                     LEKQKEGKKRMKAVGSVEVPQEAFLAILRVGEE"
     gene            3560..4927
                     /gene="bioA"
                     /locus_tag="VN647_10695"
     CDS             3560..4927
                     /gene="bioA"
                     /locus_tag="VN647_10695"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HWG97541.1"
                     /translation="MARKPTVRQLKDWDRRYLWHPFTQMQEWEQEELLIIERGKGSYL
                     IDTEGKKYFDGTSSIWVNLHGHRHPVLDRALKKQLDKIAHSTFLGLSNPPAIELAREL
                     IRIAPKGLSRVFYSDNGSTAVEVALKMAVQYWQQRRPEAGPKNTFLHLKLAYHGDTIG
                     AVSVGNIELFHSRFKSLLFSTLEAEPPYCYRCPLKLAYPSCAMACIDPIEQILKRRHR
                     ELAGFIIEPLMQAAAGMIPQPAGYLQRMRELCTKYDVLLIVDEVATGFGRTGRMFACE
                     HEGVTPDLMAISKGLTGGYMPLAATLTTDEVYSGFLGAYDEFKTFFHGHSFTGNPLGC
                     SVALANLQVFRQEKTLSRLFSKIKMLTQWLSPMAEMPIVGDIRQSGFMVGIELVKDKK
                     TKEPYPLSTKAGHRVASIARTKGLILRPIGNVLVLIPPLSTTAEELKKMVEILKESVE
                     TLHID"
     gene            4971..6170
                     /gene="lhgO"
                     /locus_tag="VN647_10700"
     CDS             4971..6170
                     /gene="lhgO"
                     /locus_tag="VN647_10700"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HWG97542.1"
                     /translation="MQTCDFLVIGGGVIGLCIARELRRRRTDARVLLIEKEPSCGSHA
                     SGRNSGVLHAGFYYSPDSLKAKFTRLGNERLTAYCEEKRIPLNKCGKLVVAKDAADLP
                     SLDELFRRGQLNGIELQSLTETEAKSIEPRVKTYQRALFSPRTSTVSPPQVVNAMQQD
                     ALREGIRIQCGAAYRGRDNRKVRTNQDGIEAGYVVNAAGLYADKIAMDYGFSEKYRIL
                     PFKGLYLYSDEPPGAIRTNIYPVPDLRNPFLGVHFTITADGKAKIGPTAIPALWRENY
                     QGLGNFNLRELAEVASRGLGLLTGAGFDFRRLAMEEIAKYSRSKMVSLASVLAEGVVE
                     RHYQTWGRPGIRAQLLDITKRKLEMDFVLEGDHRSMHVLNAVSPAFTCSIPFASYVCD
                     QIDKVMG"
     gene            complement(6174..6974)
                     /locus_tag="VN647_10705"
     CDS             complement(6174..6974)
                     /locus_tag="VN647_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWG97543.1"
                     /translation="MPDARPPLITLLTDFGERDSFVASMKGVILSINSYAAVVDLSHQ
                     IASHQIQEAGYFLKSCYRYFPEKTIHVAVVDPGVGTERRALLVSAAGSFFVGPDNGLF
                     TEVLEQEVGAKVWQINSPQYRLETVGSTFDGRDVFAPAAAWLSKGVPPSFFGPAVHDP
                     IRRSVATPVWHEELLIGKIISVDRFGNLISNITARQVREFRSAMGQTVEIHIGTYIIN
                     ELVGSYSQGSRQTPSALINSGGNLEIFLQEESAAQNLQVGVGEEVRLC"
     gene            complement(6976..7452)
                     /gene="greA"
                     /locus_tag="VN647_10710"
     CDS             complement(6976..7452)
                     /gene="greA"
                     /locus_tag="VN647_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWG97544.1"
                     /translation="MPTPITKKGYDALKAELDRLRKIERPKVIEAIAEARAHGDLSEN
                     AEYDAAKERQGFIESRLSELETKLADARVVETAGRTTETVVFGATVLVVEQESQSRKQ
                     YTLVGQDEADMKFNKISVQSPVGRALIGKRVGDFVEVKTPVKLVEYEVVEIKFEDF"
     gene            complement(7475..>8514)
                     /gene="carB"
                     /locus_tag="VN647_10715"
     CDS             complement(7475..>8514)
                     /gene="carB"
                     /locus_tag="VN647_10715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HWG97545.1"
                     /translation="YDETGLLEYMDSAVKASPNHPVLIDKYLADAIEVVADAISDGDT
                     VVVAGIMEHIEEAGVHSGDSACSLPPYTLDKPIVRDIERQMRLLAQALGVVGLMNAQF
                     AVKDRTIYVLEVNPRGSRTVPFVSKAIGVPLAKLAMKVMMGKTLKELGFTDAPLPAHF
                     SVKEAVFPFNKFPGVDVLLGPEMKSTGEVMGIDGDFGWAFAKSQAGAGAVLPTSGTAF
                     VSVKQSDRHVALDLSKRLCALGFQIQATSGTAGYLREHGVEVGTVNKVKEGRPHIVDH
                     IKNGAVALVINTVRTASAHMDSLSIRREALHRGVPYFTTMRGAHAAVMGIEATAQKGL
                     AIRTLQEYHRP"
BASE COUNT         1863 a         2506 c         2219 g         1926 t
ORIGIN      
        1 gacggaccgg cacccaaggc gctgccgaat catcgctccg gcttcatcga tcgtctggtc
       61 gtcgtcacca tgtacaccgg aagcttgagc cgcctcgagg tgattcggtg tgagtacggt
      121 gacgcccttg tagtagctga aatgttcgac cttgggatcg acgacgaccg gaaccttttg
      181 cgaggcggcg agtcgcgtca tttcggacat gagcgccggc gacacgacgc ccttggcata
      241 gtccgatacc actacgcagg acaactcctt gatccgagat tccacatatc ggagaatctt
      301 ttgccgcagc gcagttttca gttcgcttcg tccctcgatg tcataccgca caatctgctg
      361 attgtgcgcg atgacccgac tttttctggt cgtcgggcga tcatgatcga tcaccacacc
      421 cccgcggttt gatcgtttgt cgccgagttc cttcatcagc agacgtccgc tttcatccga
      481 tccgatcaca ccacagagat cggccttccc tcccagcgcc aagatattgt tgaacacgtt
      541 ggccgctcca ccgagccgga gcgattccga ctcgacatgg actaccggaa ccggcgcttc
      601 cggagagatc cgactgactc gccccatcac atagtgatcg agaatgaggt cgccgacgac
      661 gaggactgat gtttgtggaa atcgttgaag atactgccgg agagtcttgg gcgacggaac
      721 ttcactcttc ccattcgact tctctctcgg aagagacgcg ctgctgcttt tctttactga
      781 atcgccttgg cgaatcgttc ccaccgtacc cactccgtcc agtttccttg tccattccag
      841 gaccaataga tacggaacgc cttcccacgg attttttctt cgcgcacgta tccccagaac
      901 cgactgtcca gactttggtc gcgattatct cccatgacga agtaggatcc ctcggggatg
      961 gtcaccggtc cgaaattatc gcgaggattg accgtgccgt cgatcacgcc ggggtcgatc
     1021 cgttgtgtaa aagccttgtc gtcgagcgcc cggccgttca cgagcaccac cttattccga
     1081 agctgtacgg tatcccccgg cagcccgacg atgcgcttga tgaaatcttt ttcttcatcc
     1141 tctggaaacc gaaacacgat gatgtcgccc cgctgcggct tgccgaatgc caccaccgtc
     1201 ttagaggtat agcaattgac cgggggaaac gtccattgca tctggcaatc cgtcggccac
     1261 tgaagaccat aggaaagttt gctgaccaaa atgtgatcgc cgatcagcaa ggtaggaatc
     1321 atcgagcctg atggaatctt aaatgcctgc acgacgaaca cgcgaatggc gaatgccagc
     1381 agcatcgcca cgatgatcgc ttccgcatac tcccggacga tggatttccg tcccgattgg
     1441 tccatgttgt ccgcaagctt ggctcccgga agcgaggcct gctctcctcc ccgcggagaa
     1501 ccgggcagct tctccacatt tctctgattt tgatcgaggc tcattcttct ccgactctca
     1561 agatggccaa gaacgcttcc tgtgggactt cgacgctgcc gaccgccttc atccgtttct
     1621 ttccttcttt ttgtttttcc agcaacttcc gtttccgcgt gatgtcgccg ccgtaacact
     1681 tggccgtcac gttctttttc atcgccccga tggtttcccg ggcaacgatc ttgttcccga
     1741 tcgccgcttg aatggcgatt tcaaacatct gccggggaat caactctttc atcttctcag
     1801 ccatttggcg gccgcgatgg tacgaacgtt cgcgatgcgt aatgaaagac agggcatcca
     1861 ccgcctcacc gttgagcaag atgtcgatct tcaccagatc cgactctcgg tatccgagta
     1921 attcatagtc cagtgaggca tacccttgtg ttctggactt caacttgtcg taaaaatcca
     1981 ggatgacttc attcagcggc agttcgtagc tgatcatcac acgggtagga tcgagaaatt
     2041 gcaagctacg ctgaatgccc ctccgttctt ggcagagctg gagaatggac cccatatatc
     2101 gctcgggggt aatgactgtc gcaagaataa acggttcttc aaacgaggcg atattgttgg
     2161 gcggaggcaa ttccgccgga ttgtcgatct ccagcttgtc acccttcgtc gtggtcacgt
     2221 gatagaccac ggtcggcgcg gtggtaatca gtgtgaggcc atactcccgt tccaaccgtt
     2281 cctgaatgat ttccatgtgc agcaagccca ggaaaccaca gcggaacccg aagccgagcg
     2341 ccaacgatgt ttccggctcg tagacgaacg atgagtcgtt gagccgaagt ttgacgaggg
     2401 cgtcccgcaa gtcttcgtac ttggccgtgt ccgttgaata cagtccgcaa aacaccagag
     2461 gcttcacttc tttgtatccg ggaaacggcg cggcggtggg agtcactgca tccgtcaggg
     2521 tatcgccgat cttgacgtcg gcgacttctc tcatgttggc gcagagatac ccaacttccc
     2581 ccgtcaacag ttcggccttc ttggtccgtt tgggagtgaa ttgtcccacc tccatgacct
     2641 caaaggtccg gtcattcgac atgactttta tcttcatgcc cggccgaatc gatccatcca
     2701 ccaatcgcgc caggacgatc acgccttgat aattgtcgaa ccaagaatca aagatcagcg
     2761 ccttaaaggg cgcctgcgga tcgccggagg gaggagggat ccgcgcaatg accgcttcca
     2821 gcacctccga tacaccttta ccttccttgg cactgatggg cagggcctcg gcggcctcga
     2881 gctgcagcac ttccgagatc gactgtttcg tcccctcaac atcggcactg gccagatcga
     2941 tcttattgat gaccggaata atcgtgaggt tattggccat cgccaaattc acgttggcaa
     3001 tcgtctgcgc ctgcacgccc tgagtggcat cgattagcaa gagtgctcct tcacaagccg
     3061 ccaagcttcg ggagacttca taggtaaaat cgacgtgtcc cggcgtatcg atcaaatgca
     3121 gggcatacgt cttcccatcc cgggctttgt accgaatggc cactgcatgg gctttgatcg
     3181 taatgccacg ttcccgctcg aggtccatgg catcgaggat ctggtctttc gcctctcgag
     3241 cagtgactgc gccagtagct tctaggaacc ggtcagcgag ggttgatttg ccgtgatcga
     3301 tatgagcgat tatggaaaaa ttgcgtataa ggctttgcaa atcctagctc atccgtgaaa
     3361 atcggttcat tatagtaact gcctcccagg ttgtcaaaga gatcgcctcc cgcctataat
     3421 cacgcgccgc gccgaaacaa tgtgaagagc tcatcgcgta tgacgtatga cacagttaga
     3481 taagacccag ctaaggacgt tgcactcctc ggctccgcaa tatgctagaa gccgttagct
     3541 ccgtgccctg atttccaccg tggctcgtaa accaaccgtt cgccaactga aggactggga
     3601 ccgtcgctat ctctggcatc cgtttaccca gatgcaagaa tgggaacagg aggagctgct
     3661 gattatcgag cgcggcaaag gttcctacct tattgatacg gagggaaaaa aatatttcga
     3721 tggcacctcc tccatctggg ttaatctcca tggacatcgg catccggttc tggaccgtgc
     3781 actcaaaaag caactcgaca agattgccca ttcgacgttt ctcggcctct ccaatcctcc
     3841 tgccattgag ctggcccgcg agttgatccg gatcgcgccg aagggactct cacgcgtctt
     3901 ctattcggac aatggctcta ccgcagtcga ggttgcactg aagatggccg ttcaatactg
     3961 gcagcagcga cgccctgaag ccggccccaa aaataccttt ctccatctga agctggccta
     4021 tcacggagat accatcggcg ctgtgagtgt cggcaacatc gagttgttcc actctcggtt
     4081 caagtcgctg ctgttttcga ccctagaagc agaaccgccc tattgctacc gatgtccgct
     4141 caaactcgct tatccttctt gcgcgatggc atgcatcgac ccaatcgaac agatcttgaa
     4201 acgtcgtcat cgtgaactgg ccggcttcat catcgagccg ctgatgcaag cagctgccgg
     4261 tatgattccg cagccggccg gttatctgca acgaatgcgc gagctctgta cgaaatacga
     4321 tgtcttattg atcgtcgatg aagtggcgac gggattcgga cgaacgggcc gaatgttcgc
     4381 ctgcgagcat gaaggcgtga cgccggatct gatggcgatc agcaaggggc ttaccggcgg
     4441 ctatatgccc ctggcagcaa cattgacaac ggatgaggtc tacagcggtt ttctcggagc
     4501 ctacgatgag ttcaaaacct tttttcatgg acacagcttt accggcaatc cgctgggctg
     4561 ttccgtggcc ctcgccaatc tccaagtctt tcgccaagag aagacgctgt ctcgactgtt
     4621 ctcaaagatc aagatgctga cccaatggct gagcccgatg gctgaaatgc ccattgtcgg
     4681 tgacatccgt caaagcgggt tcatggtggg aatcgaattg gtcaaagata aaaagaccaa
     4741 ggaaccttac ccgctcagta ccaaggccgg ccatcgcgtg gcatcgatcg cgcggaccaa
     4801 agggctgatc ttgagaccga tcggcaatgt actcgtcctt ataccaccgc tctcgaccac
     4861 cgccgaagaa ttaaaaaaaa tggtggaaat cctcaaagaa tccgtggaga ctctgcacat
     4921 tgattgagcg tcgcttcgat gctcacgaat caggagaagc accaaaccac atgcagacct
     4981 gtgattttct tgtcattgga ggcggggtga tcggcctgtg cattgcccgt gaactccgaa
     5041 gacgtcggac ggatgcccgt gtattgttga tcgaaaagga accctcgtgt ggctcccatg
     5101 ccagcgggcg caatagcggc gtgctccatg ccggtttcta ttactcgccg gacagcctca
     5161 aagccaagtt tactcgcctc ggaaacgagc ggctcaccgc ctattgtgag gaaaagcgaa
     5221 tccccctcaa taaatgcggc aagctcgtcg tcgcgaaaga cgcagcggat ttgccctcgc
     5281 tcgatgaatt attccgacgc ggacaactca acggcattga gctccagtct ctgactgaaa
     5341 cggaagccaa atccatcgag ccgcgcgtga aaacatacca acgagcgctc ttctccccac
     5401 gcacctcgac ggtcagtccg ccccaggtcg tcaatgcgat gcagcaagat gcactgcggg
     5461 aaggtattcg aattcagtgc ggcgcggcct atcgcggacg ggacaacagg aaagttcgga
     5521 cgaaccagga cggcatcgaa gccggatacg tcgtcaatgc cgcgggtctg tatgccgaca
     5581 aaatcgccat ggactatgga ttttccgaaa agtatcgcat cctgcctttt aagggtctct
     5641 acctctattc cgatgaacca cccggtgcga tccgcaccaa tatttacccc gttccggatc
     5701 tcaggaatcc gtttctgggt gtccatttca cgatcacggc cgacggaaag gccaagatcg
     5761 gtccgacagc cattccggct ttatggcgcg aaaactatca ggggctcggc aacttcaact
     5821 tgcgtgaact cgccgaagtc gcgagccgag gactggggct gttgaccgga gctggattcg
     5881 atttccgacg cttggcgatg gaagaaatcg caaaatactc acgaagcaag atggtgtcgc
     5941 tggcctcggt acttgccgag ggcgtagtcg agcgtcacta ccaaacatgg ggccgtcccg
     6001 gaatcagagc ccaactgctc gacatcacga agagaaaact tgagatggat ttcgtcttgg
     6061 aaggcgacca tcgctccatg cacgtactca acgccgtctc tcccgccttt acctgttcga
     6121 tccccttcgc aagctatgtt tgcgatcaga tcgacaaagt gatggggtga ggatcaacag
     6181 agacgtacct cttcgccgac gccgacttgc aggttttgag ctgcgctttc ttcctggaga
     6241 aagatttcca agttcccgcc gctgttgatc aatgcggatg gagtctgacg actcccctgg
     6301 ctgtagcttc cgaccaattc gttaatgatg tacgtgccga tatggatctc cacggtttgt
     6361 cccattgctg atcggaattc ccgaacttgc cgcgccgtga tattggaaat gagattcccg
     6421 aaccgatcga ccgagatgat ttttccgatc agcaattcct catgccaaac cggggtcgcc
     6481 acggaacgtc ggatcggatc atggaccgcc ggtccaaaaa acgacggtgg gactcctttg
     6541 ctcagccatg cggcagccgg tgcgaacaca tcccgaccgt caaaggtcga cccgactgtt
     6601 tccaaacgat actgcggact gttgatctgc cacaccttcg ccccgacctc ctgctccagc
     6661 acttcagtaa acaacccgtt gtccggaccg acaaagaacg agccggccgc agaaacaagc
     6721 aacgcccggc gctccgtccc gactccggga tcgaccacgg cgacgtggat ggtcttttcc
     6781 gggaaataac gatagcagga cttcaggaag tatccggctt cttggatctg gtgagaggcg
     6841 atttggtgtg aaagatcgac gacggcggca taggaattga tcgacagaat gacccccttc
     6901 atgctggcga caaaagaatc acgctcgccg aaatccgtca gcagggtgat gagggggggg
     6961 cgggcgtccg gcacgtcaga agtcctcgaa tttgatctcc acaacctcgt attcgaccag
     7021 cttgacgggg gtcttcactt ccacgaaatc tccgacacgc ttcccgatga gcgcgcgacc
     7081 gaccggggac tgtacggaga tcttgttgaa tttcatgtcg gcttcgtcct gcccgactaa
     7141 ggtatattgt ttccgcgatt gggactcctg ttcgacgacc aacacggtcg caccgaagac
     7201 gaccgtctcg gtggtgcgtc cagccgtttc caccacgcgg gcatcggcga gtttcgtctc
     7261 gagttctgag aggcgtgact caataaaccc ctggcgttct ttggcggcgt catactcggc
     7321 gttctcgctg agatcgccgt gagccctcgc ctccgcaatc gcttcgatca ctttcggccg
     7381 ctcaatcttg cgcaaacgat ccaattccgc ctttaacgca tcatagcctt tcttcgtaat
     7441 gggggtcggc atgctccaat cgctcctgaa caatttatgg ccgatgatac tcctgcaatg
     7501 tgcgaattgc taagcccttt tgggcggtcg cttcaatgcc catcaccgcc gcatgggcac
     7561 ctctcatggt cgtgaaatac ggaacgcctc ggtggagggc ctcccgcctg atggacaagg
     7621 aatccatgtg agcagaggcg gtccgcaccg tgttgatgac gagcgccacc gcgccattct
     7681 tgatatggtc gacaatgtgg ggacgtcctt cctttacttt attgaccgtc cccacttcga
     7741 cgccgtgctc tctcagatac ccggctgttc cgctggtcgc ttgaatctga aacccgagcg
     7801 cacataaccg cttcgagaga tccaacgcga catgacgatc ggattgcttc acgctcacga
     7861 aggctgtccc ggatgtcggg aggaccgcgc cggctcccgc ttgggacttt gcgaacgccc
     7921 atccgaaatc tccgtcgatg cccatcactt ctccggtcga tttcatttca ggccccagca
     7981 gcacgtcgac ccccgggaat ttattgaacg gaaagaccgc ttctttgacc gagaaatgtg
     8041 ccggtagagg cgcgtccgta aagcccagct ccttgagggt ttttcccatc atcaccttca
     8101 tcgccaactt ggcgagcggt acgccgatcg ctttactcac gaacggtacg gtccgagatc
     8161 cccgaggatt gacttcaagc acatagatgg tccggtcttt cacggcaaac tgtgcattca
     8221 tcagtccgac gacgccgagt gcctgcgcca gcaagcgcat ttgccgctcg atgtcgcgca
     8281 cgatgggctt gtccaacgta taaggaggca gcgaacaggc tgaatcgccg gaatgaaccc
     8341 cggcttcttc gatatgctcc atgatccctg caaccaccac ggtgtcgccg tccgaaatcg
     8401 catcagcaac gacttcgatg gcatcggcga gatacttatc gatcaagacc gggtgattgg
     8461 gggacgcctt gaccgcggaa tccatgtact ccagcaagcc tgtttcgtca taca
//
LOCUS       DATNDD010000442         2744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1771251, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000442 DATNDD010000000
VERSION     DATNDD010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771251"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1225)
                     /gene="gmd"
                     /locus_tag="VN647_10720"
     CDS             complement(218..1225)
                     /gene="gmd"
                     /locus_tag="VN647_10720"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWG97546.1"
                     /translation="MKKALITGITGQDGSYLSELLIGKGYEVYGIIRRSSSFNTGRID
                     PIYEDPHVPHRRLHLVYGDLNDASSLNRILRTVQPDEIYNLGAQSHVRVSFDIPEYTG
                     EITGLGTIRLLEAIRESGLKPKFYQASSSEMFGKVLEVPQRETTPFYPRSPYGAAKVY
                     AYWITVNYREAYGLYACNGILFNHESPRRGETFVTRKITKAAARIKLGVQQDLFLGNL
                     EAKRDWGYAGDYVEAMWMMLQAQTPDDYVIATGETHTVKDLLELTFDRLQLDWKKHVK
                     IDAKYYRPTEVDLLIGDAGKAKKHLGWQPKVRFEELVAMMVDADLAAEREKLEGTRKR
                     T"
     gene            1374..2288
                     /locus_tag="VN647_10725"
     CDS             1374..2288
                     /locus_tag="VN647_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97547.1"
                     /translation="MAAIPPQRVRRISLFLCLVVCALLIAHSINAFVAEALYVIPTHS
                     VGSGDRSTGSSLPASYSPTQAAEDVRSSGLFLLPAAPQGGLTNSDRRGGGPSGAPVRA
                     SLGLAARIRLIGVVLGDQRGMFAIAEELASKQQTLYRVHDQVLDLGEVSEIRRNGILV
                     RQGNLEELLELAQSENQAVPGGSAGTAAAVAQTQTAGVPLRKVIDRREVEQAMSDLPK
                     LLSQARAAPHMVNGAIGGFRLDYIAPTSFYEKIGVQTGDVLQRVNGVDIRDPSTMLNL
                     LQQLKNEQIVKLDVLRNNQRSTITYELR"
     gene            2539..>2744
                     /locus_tag="VN647_10730"
     CDS             2539..>2744
                     /locus_tag="VN647_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97548.1"
                     /translation="MRGQGQLSEQLDNDQSGPTVRRPLLGRILGEKFNLLDAKLEEAL
                     AYQQEKGGLLGEVLLHLRLLREEQ"
BASE COUNT          588 a          785 c          741 g          630 t
ORIGIN      
        1 agatgaatca ccaagtccgg cttcgcatca cgatataatt gcttcaccgc gtccatctgc
       61 accagatcgt agtcttgact tcgaggcacg acgatttgcc ggcatccctt cgcgcgcaac
      121 tgatcgacga cgaatgaacc gagaaacccg gctcctccgg tgaccaccac tcgcgtctcc
      181 ttccagaatg aaggcatccc tgattaccct tcgcgctcta ggttcgtttt ctggtccctt
      241 ccaacttttc tcgttccgct gcaagatctg catccaccat catggccacc aattcttcga
      301 agcgcacttt aggctgccag ccgaggtgtt tcttggcctt gccggcatct ccgatgagca
      361 gatcgacttc ggtcggccgg tagtacttgg catcgatctt cacatgtttc ttccagtcca
      421 gttgcagccg gtcgaacgtc agctccagca ggtccttcac ggtgtgcgtt tcgcccgtcg
      481 cgatgacata atcatccggc gtctgagctt gtaacatcat ccacatggct tcgacatagt
      541 cgccggcata tccccagtcg cgcttggctt ccagattccc cagaaacaag tcttgttgaa
      601 cacccagctt gatgcgagcg gcggccttgg tgattttccg cgtcacgaag gtttcgcccc
      661 gacgcggcga ttcatggttg aataggatgc cgttacaggc atacaggcca taggcctcgc
      721 ggtagttgac cgtaatccag taggcgtaga ccttagccgc tccgtacggg ctcctcggat
      781 agaagggggt ggtttcccgt tgcggcacct ccagcacctt gccgaacatc tcgctggatg
      841 acgcctgata aaatttcggc ttgagccccg attcccggat ggcctcaagc agccgaatgg
      901 tgcctaatcc tgtgatctca ccggtatact caggaatatc gaagctgacc cggacatgac
      961 tctgcgcgcc aagattatag atttcatccg gctggacggt gcgcaaaatt cgattcaggg
     1021 aactggcgtc gttgagatcg ccatagacca ggtgaagtcg ccgatggggc acatgcggat
     1081 cttcatagat cggatcgatg cgaccggtat taaaggaact ggaacggcgg atgatgccgt
     1141 agacttcgta tcccttcccg attagaagct ccgacaagta cgacccatct tggcccgtaa
     1201 tcccggtaat caatgccttc ttcacgtgta aaccctcctt gagcaagaaa cggattattg
     1261 cgtgctgcga tccgtttgtc tactcctcca tgcaaaagac cgttgatgaa agattcttgc
     1321 gaccgacgat atcaaccatg tacgatgggc cagatagaat cagaggcaat atcgtggcag
     1381 caattcctcc acaacgagtg cgtcggatca gcctattcct ctgtctagtc gtgtgcgcgc
     1441 ttctgatcgc ccactccatc aatgcctttg tcgccgaggc attgtatgtg atccctacgc
     1501 attcggtggg ttccggcgat cgatcgacag gctcttcgtt gcccgcctcc tattcgccga
     1561 cccaagcagc ggaggatgta cggtccagcg gtctctttct cctccctgct gcgcctcagg
     1621 gtggactcac gaactctgat aggcgaggag gaggaccctc cggagccccg gttcgcgctt
     1681 cgctcggcct ggcggccagg attcgcttga tcggtgtcgt gctcggcgat cagcgcggca
     1741 tgtttgcgat cgccgaggag ttggcctcca aacaacagac gttgtatcgc gtccatgacc
     1801 aagttctcga tctgggagaa gtgagtgaga ttcggcggaa cggaattctc gtgcgccaag
     1861 gcaacctgga ggaattattg gaactggcgc agtcggagaa ccaagcggtt ccaggcggtt
     1921 ccgccggtac cgcggctgct gtcgctcaga cacagaccgc cggcgttcct ctacgaaaag
     1981 tcatcgaccg ccgtgaggtg gagcaggcca tgagcgatct gccgaaactc ttatctcagg
     2041 ccagagccgc acctcatatg gtgaacggag ccatcggcgg gtttcgcttg gactatatcg
     2101 ctccgaccag cttttatgaa aaaatcgggg tccagacagg agatgtgttg cagcgcgtca
     2161 acggcgtcga cattcgcgat cccagtacca tgttgaattt gctgcaacaa ctgaagaatg
     2221 agcagatcgt caaactcgat gtgctccgca acaatcagcg atcgacgatc acgtacgagc
     2281 ttcgctgaga cagaggtcgg cgctcttccc ccacaatttc catgatcgct tgagtcactc
     2341 cctctcgacg accgacgaat cgacttctgc aatagcgggt tgatggcgag gaatactcag
     2401 ggtctaggac ccacaagggt tcgtcctttg cgttaagaat tcgaggtgtt cttgccaggc
     2461 cgtcggaagg gagagtatct ttacgaccga gatggaagcg catgagagaa gcgactatga
     2521 tggcgctcat ctctattcat gagaggtcag ggccaattgt ccgaacaact cgacaacgat
     2581 cagtccggtc ctactgttcg tcgccctcta ttgggacgca ttctcgggga aaagttcaac
     2641 ctcttggacg caaaactgga agaggccttg gcctaccagc aggagaaagg cggcctgctg
     2701 ggagaggtcc tgctgcattt gcgtttactg cgggaagagc aatt
//
LOCUS       DATNDD010000443         2996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2474803, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000443 DATNDD010000000
VERSION     DATNDD010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474803"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VN647_10735"
     CDS             complement(<1..761)
                     /locus_tag="VN647_10735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWG97549.1"
                     /translation="MKTLETELAPPFYATTWRWMTPVVSGAALGAIALAIPPVREIFQ
                     LEGLRWLHVFLFAFLGTGALTPMMVRIGHRWNLVDTPADRKIHITPTPRLGGLALYGG
                     FIGSVLLNSIVPDWMVAVLMAGSLLLIIGVIDDIRELPAWVKLCGQLLAAGIVIASGK
                     ILTLFPDGSLGDAANILLTLFWIVGITNAFNFFDGMDGLAAGLAVLMAGFMGIVAFET
                     DQAGLGWLAIAMIGAGLGFLPYNFRGTKPAVIFLG"
     gene            complement(758..1831)
                     /locus_tag="VN647_10740"
     CDS             complement(758..1831)
                     /locus_tag="VN647_10740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWG97550.1"
                     /translation="MVAMLIPHSRPFIETDEVQRAIEVLRSRRLAQGGMVEQFERGMA
                     AYFGSAGGVAVSSGTAALEIALRALGIGPGDEVVLPSYVCAAPWLAIQRVGAMPRVVD
                     IDLETFAIDPTATRQALTSKTKAVIVPHMFGLPADLTTLERLGVPIIEDCAQTLGAME
                     RGRIVGSVGLLTICSFYATKLLCTGEGGMVLSRDESLLERVRMLREYDEAPSLCPGAT
                     NVKMTDLQAAIGLVQLDRMACFLERRNWLADEYRATLDGTGLVQPIVPSGRTHVYYRF
                     VVRMPDLYSQVNGVSSVIARFEGRRVQCRKPVFRSLHRYLDLDGFPASEEAERTALSI
                     PLFPSLTDEDVAQVHEALRSEWS"
     gene            complement(1834..2481)
                     /locus_tag="VN647_10745"
     CDS             complement(1834..2481)
                     /locus_tag="VN647_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWG97551.1"
                     /translation="MKNANVNEPMRILALGAHPDDIEVGCGGTLIKYAENGHRIFLMV
                     MTQGGAGGNVSVRKREQERSAELLQAEEVFWGGYQDTEVPLGRDLIQTVEEIVRKIEP
                     HFIFVHYHDDTHQDHRNLAMSTITATRYTKNVLFYEGPTTQNFAPTVFVDIQQALDRK
                     IQALEAHASQVKKTNIEGIDIADLVRSSAHFRGIQGRVKTAEGFLPLRLFINIGV"
     gene            complement(2493..>2996)
                     /locus_tag="VN647_10750"
     CDS             complement(2493..>2996)
                     /locus_tag="VN647_10750"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HWG97552.1"
                     /translation="VLINPTTAWKAQHLRALDIHYARAPYRDRYLSELQELYARPWSR
                     LNDLNKATVQWLLAAYGITTPVHSAAEYAARDEPTDRLIDLCRVVGASQYLAGPGAEH
                     YMDKPRFESSGMRLKIQVFQHPIYRQVYEPFESNLSALDLLLMQGPNAITTLRRARSM
                     DYSMTSV"
BASE COUNT          694 a          894 c          787 g          621 t
ORIGIN      
        1 tcgcccagaa aaatgacagc cggctttgtc cctcgaaaat tgtacggcaa aaagccaagg
       61 cctgctccga tcatggcgat cgccagccag cccaaccctg cctgatcggt ttcaaatgcc
      121 acgatgccca tgaatcctgc catcagcacg gcaagaccgg cggccaatcc atccatgccg
      181 tcgaaaaaat tgaaggcgtt cgtaatcccg acgatccaaa aaagcgtcag aagaatgttg
      241 gccgcatctc cgagtgatcc gtccggaaag agtgtcaaga tcttgcccga ggcaatcaca
      301 atacccgctg ccagcaattg tccacacagt ttcacccagg ccggcagctc tcgaatgtca
      361 tcgatgacgc cgatgatcaa cagtagagag ccggccatca atacagcaac catccaatcg
      421 ggcacgatcg agttcaacag cacggagccg ataaatccgc catataacgc aagaccgcca
      481 agccgcggag tgggggttat atgaatcttt cgatcggcag gtgtatcgac aaggttccat
      541 cgatggccga tccggaccat catcggggtc agggctccgg tgccgagaaa cgcaaagaga
      601 aagacatgca gccatcgcag gccttccagc tgaaagatct ctcgaacagg aggaatggcc
      661 agagcgatgg ccccgagtgc cgctccggat acaaccggcg tcatccagcg ccacgtcgtg
      721 gcgtagaatg gaggagccag ttcggtctcg agtgtcttca tgaccactcc gaacgcagcg
      781 cctcatgcac ctgggccacg tcttcgtccg tgagcgacgg gaacagcggg attgagagcg
      841 cggtacgctc ggcttcttcc gaagcgggaa atccgtccaa atccagatag cgatgaagcg
      901 aacggaacac cggcttccgg cattggaccc tccggccttc aaatcgtgcg atcacactgc
      961 tcacgccgtt cacctggctg tacagatccg gcatgcgcac cacgaaccgg tagtacacat
     1021 gcgtacggcc ggacggtacg atcggctgca caagtccggt accgtccagt gtcgctcggt
     1081 attcatccgc aagccagttt cgccgttcga ggaagcatgc catgcggtcc agttgcacca
     1141 agccgatcgc ggcctggaga tcagtcatct ttacattcgt cgctccagga cagagggacg
     1201 gtgcctcgtc gtattccctc agcatgcgca ctcgttccaa caaactctcg tcgcgggaca
     1261 gcaccatccc tccttcaccg gtacagagca gcttggtcgc gtaaaaggag caaatcgtta
     1321 aaagaccgac tgatcccacg atccttcctc gttccatggc acccagtgtc tgcgcacagt
     1381 cttcaatgat cggcactccc aatcgttcaa gggtcgtcaa gtcagcggga aggccgaaca
     1441 tatgggggac gattacggcc ttggtcttcg aggtaagagc ctgtcgcgtt gctgtcggat
     1501 caatcgcaaa agtctccaga tcgatatcga cgactcgcgg catggctccg acacgctgaa
     1561 tggccaacca aggagccgca cagacataac tgggaaggac gacctcatcg cctggcccga
     1621 ttcccaatgc tcgaagcgca atttccagcg ccgccgtccc ggagctgacg gcgactccgc
     1681 cggccgatcc aaaataagcc gccattccac gctcgaattg ctcgaccatg cctccttggg
     1741 ccaaccggcg agatcgcaat acttcgattg ctctctggac ctcatccgtt tcgataaagg
     1801 gtcgtgagtg aggaatcagc atggcgacca tcgtcatact ccgatattga tgaacaatcg
     1861 caaaggcaaa aagccttcgg cggttttcac gcgaccctgg atcccgcgaa aatgcgccga
     1921 cgacctcacg agatccgcga tgtcgatccc ttcgatattg gttttcttca cctgggaggc
     1981 atgcgcttcc aatgcctgaa tttttcgatc caacgcttgc tggatgtcca caaaaaccgt
     2041 gggtgcgaag ttctgcgtag ttggtccttc ataaaacagc acgtttttgg tgtaccgcgt
     2101 ggcggtaatc gtgctcatcg ccaaattccg gtggtcttga tgggtatcat catggtagtg
     2161 aacgaagata aaatgcggct cgatcttccg cacaatctcc tcgacggttt ggatcagatc
     2221 tcgtccaagg ggaacctccg tgtcctggta gcctccccag aacacttctt cggcttgaag
     2281 gagttcggcc gaccgctctt gttctctttt acgaaccgat acattgccgc ccgccccgcc
     2341 ttgtgtcatc accataagaa agatgcgatg gccgttctcc gcatacttga tcaatgttcc
     2401 accacacccg acttcgatgt cgtcaggatg ggctcccagt gcaagaatgc gcatcggttc
     2461 gttgacgttg gcattcttca tacctgatcc tcctagacgc tggtcatcga ataatccatc
     2521 gatcgcgccc ggcgcagtgt cgtgatggca ttagggcctt gcatcaataa gagatccagc
     2581 gcggacagat tcgattcaaa cggctcgtac acctgacgat agatcggatg ctggaacacc
     2641 tgtattttta gcctcatacc tgaagattcg aatcgcggct tgtccatata gtgctcagca
     2701 cctggtccgg caagatactg cgaggcaccg accacccgac acagatcgat caaccggtca
     2761 gtcggctcgt cgcgagccgc gtattcagcc gcgctatgga ccggtgtggt gattccatag
     2821 gccgcgagca gccactgcac ggtcgccttg ttgagatcat tcagcctaga ccagggtcgg
     2881 gcgtacagtt cctgcaactc ggaaagatac cggtcgcggt atggtgcccg agcataatgt
     2941 atatccagcg ctcggaggtg ttgagctttc catgcggtag tcgggttaat cagaac
//
LOCUS       DATNDD010000444         3285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2787813, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000444 DATNDD010000000
VERSION     DATNDD010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787813"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..492)
                     /locus_tag="VN647_10755"
     CDS             complement(115..492)
                     /locus_tag="VN647_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97553.1"
                     /translation="MGNIFKKLGIGLVVGGALILVGHSQELPISFQVLFFIVALIGAI
                     VFILLDAQPIRTMSGGKSLFAVIAFWILLVTVCVAGASLLPQFDPEDERAKIDKLLGK
                     ERVASQKGKAEELLLAPRPSMNR"
     gene            complement(532..2241)
                     /locus_tag="VN647_10760"
     CDS             complement(532..2241)
                     /locus_tag="VN647_10760"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWG97554.1"
                     /translation="MKKLSSGLLALVVLCSLCVTATGVYAQEVGASSVEFPYTGNRTA
                     VWVVAQLHILFAAFILGAPIFVVISEWLGYRKQDPRYDRLAREITKVTVILFSMTAVT
                     GGLFIFVLLAAYPQFTTSFINQFYMVFAVMYPALFIAETILMYAYLYTWDVWKGEKKG
                     RHIALGVLLNLVCLLILFVINGPTSFMNTPPKAEGVSPQDFIAAATLWDKIANQSWFP
                     LSLHRIDGNVAFGGFITGMIAAYMYMGSKTQEERAYYDWMGFAAIWIAVGGSLLQPFT
                     GLLLAYEMCDYDFSFCPYMMADQLSMFFETQGIMIGLLFLGINYYSWLSVKRIEGAEN
                     VRMTILAPIVLIVLLPATMWVMDKFWIPDPMSLAFLLPLTLLPFLLGRFIPKTVSART
                     VIKIGFIIMLISDAVWLTPAGFVATGTDMPDDMKLPEGWDYLSSMPAKLSAILTLVYV
                     TVVNYVLYNRTIKQGTILWGKIDFAAQFVLIFLAFTSTWIMGLMGAVRSLLKKYFHVY
                     SLVSDLSAESFTPTLSYSAGWITAITVVFIAIVSVAALVALRPSVARVREPEGSPVPI
                     AAK"
     gene            complement(2544..3023)
                     /locus_tag="VN647_10765"
     CDS             complement(2544..3023)
                     /locus_tag="VN647_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWG97555.1"
                     /translation="MSMRIGSIHAVLIMSLGLSSCGEQGEGPIVPPPPAPAEYADKHM
                     PAEWWGDAQKLEEGRKLFLGETNPDVNCAGCHGKDGKPVKAGATDFRNPERMKLYSDS
                     VWFWRISEGVANTKMKGWKSKLSEEDRWKLVLYERNFGLAGKTWNEEKKQWGDAVAK"
BASE COUNT          831 a          986 c          799 g          669 t
ORIGIN      
        1 aatcgccggc gcccgtgccg gccgccgcag caggcttttc tccagccgat ggagccggag
       61 ctgtcgccac agcttgacca ctacccaacc ccttcagtcg ctcttccaag actctcacct
      121 gttcatcgag ggccttggcg cgagcaatag ctcttcggct ttccccttct gagaggcaac
      181 ccgctctttt cccaaaagtt tgtcgatttt ggcccgttca tcctcagggt caaactgagg
      241 taacagcgat gcgccggcaa cacagacggt caccaagaga atccagaatg cgatgacggc
      301 aaagagggac ttccctccgc tcatcgtccg gatcggctgc gcgtcaagca gtatgaaaac
      361 gatcgccccg atcaatgcaa cgatgaagaa caacacttgg aatgagatcg gcagctcctg
      421 cgaatgtccg acgagaataa gagcgccacc aaccaccagc ccaatgccta gcttcttgaa
      481 tatattcccc atacgaattt tcgtcgcctc agtaggcgct ccacgctgtg attatttggc
      541 tgcgatcgga acaggacttc cctcaggctc tcgtaccctt gcgacggaag gccgaagagc
      601 caccagcgcg gccacgctga cgatggcgat aaacacgacg gtaatcgccg taatccaccc
      661 ggccgaatac gaaagtgtcg gagtaaacga ctcggcactg aggtccgaca ccaaactata
      721 gacatggaag tatttcttga gcaacgaccg caccgccccc atcaacccca tgatccacgt
      781 ggaagtaaat gccagaaaaa tcaacacgaa ttgcgctgca aaatcgattt tcccccagag
      841 gatcgtgccc tgcttgatgg ttcggttata caagacataa ttgacgaccg tcacatacac
      901 caacgtcaag attgccgaaa gcttggccgg catcgacgac agatagtccc acccctccgg
      961 cagtttcatg tcatcgggca tatcggtgcc ggtggccacg aaaccggcag gcgtgagcca
     1021 gacggcatcg cttatgagca tgatgataaa gccgatcttg atgacggtcc gtgccgagac
     1081 ggtcttggga ataaaccggc ccaatagaaa cggtaatagc gtgagaggca gcaagaacgc
     1141 caacgacatt ggatcgggaa tccaaaactt atccatcacc cacatcgtgg cggggagcaa
     1201 cacgatgagg acgatcggcg ccaagatggt catccgaaca ttttcggccc cttcaattcg
     1261 tttgacgctg agccagctat aatagttgat gcccaggaac aacagcccaa tcatgatgcc
     1321 ttgcgtttca aaaaacatgg aaagctgatc ggccatcatg tacgggcaga atgaaaaatc
     1381 gtaatcgcac atttcatacg ccaacaagag cccggtaaac ggctgcaaaa gcgacccccc
     1441 caccgcgatc cagatggcgg cgaagcccat ccaatcgtag taggcccgct cctcctgcgt
     1501 cttcgacccc atgtacatgt aggcggcgat catcccggtg ataaacccac cgaatgccac
     1561 gttcccgtcg attcgatgga gactcagagg gaaccaactt tggttcgcaa tcttatccca
     1621 caaggttgcc gccgcgatga aatcctgcgg cgaaacccct tcagccttcg gcggtgtgtt
     1681 catgaacgac gtggggccgt tgatgacaaa caagatgagc agacagacga gattcaagag
     1741 cacgccaaga gcgatgtgac gccctttctt ttcacccttc cagacatccc aggtatagag
     1801 gtaggcatac atgaggatgg tctcggcgat gaacagcgcc ggatacatga cggcgaagac
     1861 catataaaac tgattgatga acgaggtcgt gaactgcggg taggcggcaa gaaggacgaa
     1921 aataaacaag ccgcccgtca cagccgtcat actgaagaga atgaccgtca ccttggtgat
     1981 ctctctagcc agacgatcat agcgggggtc ctgcttacgg taccccaacc attctgaaat
     2041 gacgacgaag atcggtgcgc cgaggatgaa cgcggcgaat aagatatgga gttgagcgac
     2101 gacccataca gccgtccgat tgcccgtgta cggaaactcc accgaagacg ctccaacctc
     2161 ttgagcgtag acaccggtgg cagtcacaca gagcgagcac aatacaacga gagccaagag
     2221 accgctcgac agttttttca cgatgcctcg atgcccccta ctttatagaa cctactctac
     2281 ggatcttgct ttcacttgta cgacaggtgc ccaggccggg ctccttgcga tttgccgtag
     2341 caccgtctca taatgacctt accgaccggc agagtatcgc agattctccc aatccttacc
     2401 gataaggaaa tcccttagca gcatacaaaa aaaacctgag cctggtcgac gacctcgctt
     2461 cgcatgcaga acgcggatgc cctcatccag gaaggttaaa gtccagaagg tgcgaagcat
     2521 cgacaaccct gtcaccctac taatcattta gcgacggcat cgccccattg cttcttttct
     2581 tcattccagg tcttcccagc cagtccaaaa ttccgctcat agagcacgag cttccatcgg
     2641 tcttcctctg agagcttgct cttccagcct ttcatcttgg tattcgccac accttctgaa
     2701 atgcgccaaa accacactga gtcggaatag agcttcatgc gctcggggtt tctgaaatca
     2761 gtggcgcccg ccttcacagg ttttccatcc ttcccgtgac agccggcaca gttgacatcg
     2821 ggatttgtct caccgaggaa gagcttgcgt ccttcttcca gtttctgagc atctccccac
     2881 cattctgccg gcatatgctt gtcggcatat tcagccggcg ccggcggtgg cggcacgatc
     2941 ggcccctcac cttgctctcc gcaactcgac agtcccagac tcatgatcaa tacagcgtga
     3001 atcgagccga tccgcatact catgacaatc cattattctg ttgtatagag agattctcgt
     3061 ccggactgca acgaggtcga gaaaggggcc gacaaacaaa aagacgcttc tgcagaggca
     3121 tcacacaccc ctgcaagagc gtcaagactg cactcgccct ttcagcttgt atcacgggcc
     3181 cccgccgacc tatcgaccga ttcggcgaga taccttgtag ccccaaggac cgtcatcggc
     3241 gatcatgatc ttttctttcc cagggatttc gccttgaccc gttcc
//
LOCUS       DATNDD010000445         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2517601, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000445 DATNDD010000000
VERSION     DATNDD010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517601"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VN647_10770"
     CDS             <1..1029
                     /locus_tag="VN647_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWG97556.1"
                     /translation="DIANELGLSVVTVSKVLRDHPDIGADTRKRVLNRMRELNYQPNL
                     AARSLVTGRSWTLGLVVPDLLHPFFADIAQAISSETSNRGYSLFISSSDENPELEVRE
                     IKQLLARRVDVIIVASAQWSIDCFRLIEEHKTPYILIDRRFQGLDANFVGVDDEAVGV
                     LATSHLIEEGCRRVAHIRGPEVSTAIGRLEGYKRALAVYNMTPLPGHIVSLGASGDHR
                     GEKGGYEAAKRLLAAHPRPDGIFCFNDPSALGAMRAILEAGVRIPEDIAVVGCGNLSY
                     SDFLRVPLSSVDQSSENIGKYTAELALKIARKKVQVRPKAELIPPRLVIRTSSLRHAH
                     VDNPRTLK"
     gene            complement(1065..>2590)
                     /locus_tag="VN647_10775"
     CDS             complement(1065..>2590)
                     /locus_tag="VN647_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HWG97557.1"
                     /translation="STSDALAGKAAAPHAALFHNNHDGTFTDESASAGVTNERWGTGC
                     VVGDYDNNGWPDLYVTNVGASRLYRNNHNGTFSDAAVQAGVALHEGSTDRMDHTGATF
                     GDYDGDGNLDLFVTGYIEIGMNDPVLSNSKIAGYNFCQYRGVKVMCGPRGLTGGRDHL
                     FHNNGDGTFTDVSKKLGVDGPNGYYGLDALFADVNNDGKPDLLVANDSTPNYLYMNQG
                     NGKFKDESYESGFALNGDGREAANMGLAVGDYENNGRLSVVSTTFSDDFPILFQNNGK
                     GAFTDVSYQAGIADASIPFVKFGDGFLDYDNDGWKDLLIVNGHVYPQVDQHPEWGQSY
                     AQRPLLYHNLGNGRFELVPAVEGTGLAVVTVGRGAAFGDLFNDGKIDVVINSMDGGPV
                     LLRNVYPNHHHWVEIKLIGGPKSPRDAVGARVDLTANGIRQRGDVLSGGSYISSNDMR
                     VHFGLGDSNKVDQVEIHWPSGSMEKIKLPFVDRIYDITENHGVTGAMCQGEPCITVH"
BASE COUNT          559 a          740 c          684 g          607 t
ORIGIN      
        1 gatatcgcta acgaactggg tctgtcggta gtcaccgttt cgaaggtgct ccgagaccat
       61 ccggatatcg gagctgacac gcgaaagcgc gtcctgaacc ggatgcgtga gctcaactat
      121 cagcccaact tggcggcccg ctcgctggtg accggccgaa gctggactct aggcctagtc
      181 gttccggacc tgctccaccc attcttcgca gatatcgcac aagccatctc atctgagacg
      241 agcaatcggg gctacagcct gttcatctcg tcttcggacg agaatccgga acttgaggta
      301 cgggaaatca agcaactcct tgcccgacga gtcgatgtaa tcatcgtagc gtcagcccaa
      361 tggtcgatag actgctttcg tctgatcgaa gaacacaaaa cgccttacat tctgatcgac
      421 cgaagattcc agggtctcga tgccaatttt gtgggtgtcg atgatgaagc agtaggtgtg
      481 ctggctacct ctcacctcat cgaggaagga tgccggcgcg tagctcacat tcgtggaccc
      541 gaagtaagta ccgcgatcgg ccggctcgaa ggttacaagc gggctcttgc ggtatataac
      601 atgaccccct tgccgggaca catagtttcg ctgggagctt caggcgatca tcgtggagag
      661 aagggtgggt atgaagctgc gaagcgcttg ctcgccgctc atcctcgccc ggatgggatc
      721 ttttgcttca acgatccttc ggcgctggga gcaatgcggg ccattctgga agcgggcgtt
      781 cgtattcccg aggatatagc tgttgtgggc tgcgggaatc tctcctattc ggatttcctt
      841 cgcgttccac tttcttcggt cgaccaaagc agcgagaaca tcggaaagta tactgcagaa
      901 ctcgcgctca aaatcgctcg caagaaagta caggttagac ctaaagccga actgattcct
      961 ccccggcttg tgatccgcac ttcaagcctc cggcatgcgc atgttgataa cccgcgtacc
     1021 ttgaagtaat cgacacaagt acacagtccg tgcgatcgat gcattcaatg cacagtgatg
     1081 cagggttcgc cttggcacat tgcgccggtg actccatggt tttcggtgat gtcgtagatg
     1141 cgatcaacaa agggaagctt gatcttttcc atgctgccgc tgggccagtg aatctcgacc
     1201 tgatcaactt tgtttgagtc acccaagccg aaatggacgc gcatatcatt cgatgagata
     1261 tagctgccgc cactcaggac gtctccccgc tgccggattc cgtttgcggt tagatcgact
     1321 cttgcaccaa ccgcatcgcg tggacttttc ggcccgccaa tgagcttgat ctcaacccaa
     1381 tgatgatgat tgggatatac gttgcggagc agaactgggc ctccgtccat actgttgatg
     1441 actacgtcga tcttgccgtc gttgaacagg tctccaaacg cggcgccgcg gccgacggtg
     1501 acgaccgcca acccagttcc ctccacagcc ggcacaagct cgaatctgcc gttacccagg
     1561 ttgtgataga ggagaggccg ctgggcatag ctttggcccc attccggatg ctgatcaacc
     1621 tgtggataaa cgtggccatt gacgatgagc agatccttcc atccgtcatt gtcgtagtcg
     1681 aggaacccat caccgaattt cacgaagggg atcgacgcat cggcaatccc ggcctggtaa
     1741 ctgacatctg tgaatgctcc cttgccattg ttctggaaca agatggggaa atcatcggag
     1801 aacgttgtgc tgacaaccga aagtcttccg ttgttttcat aatctcccac agctagcccc
     1861 atattggcag cttcccgtcc atccccattc agcgcaaacc cgctttcgta actctcgtcc
     1921 ttgaatttac cgtttccctg gttcatgtac agatagttcg gcgtcgagtc gttggccacc
     1981 aacaggtccg gcttgccgtc gttattaaca tcggcaaata acgcatcgag cccgtaataa
     2041 ccgttggggc cgtcaacgcc cagcttcttg ctgacgtcgg tgaatgtacc atcgccgttg
     2101 ttgtgaaaca gatgatcacg tccgccggtc aatccacgcg gcccgcacat caccttcacg
     2161 ccgcggtatt ggcagaagtt atagccagcg atcttggagt ttgatagcac aggatcattc
     2221 atgccaattt ctatataccc ggttacaaaa agatcgagat tcccgtcgcc gtcatagtcc
     2281 ccgaaagtag cacccgtgtg gtccatccta tcggtggagc cttcgtggag agcgacgccg
     2341 gcctgcaccg ccgcatcgga aaaggtgccg ttgtgattat tgcggtaaag ccggcttgct
     2401 ccgacgttgg ttacgtagag atcgggccag ccgttattgt cgtaatcgcc gaccacgcac
     2461 cccgttcccc atcgctcatt ggtaactccg gcacttgcgc tctcgtccgt gaaagttccg
     2521 tcatggttgt tatgaaagag tgctgcatgc ggtgcagctg ccttgcctgc cagagcgtcc
     2581 gaggtcgacc
//
LOCUS       DATNDD010000446         4510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3654409, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000446 DATNDD010000000
VERSION     DATNDD010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654409"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /gene="ybgF"
                     /locus_tag="VN647_10780"
     CDS             <1..1324
                     /gene="ybgF"
                     /locus_tag="VN647_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HWG97558.1"
                     /translation="QQSDLKKAEKDLQQQLSQTRARQSQEISTLREHELPQLRGELEK
                     ALHQARELQNKQEDFKHRSAQLEQQTKKLEQLAAKLETDSSTRYLWMQKSFETQDAKV
                     SARLDELSKAMEALKKEVIDVVQRTNEGLAKRVDVKLEGHQKELAEHQNKIEQISQKF
                     TQFNQSLTGFREALTGLNDRVGEYEQTAKHLTSRIDVDSKTTTTHAEDVNRSVISITK
                     ALEAVGKKVTVRLDEQDNRIDALAKTLEQVSNRPAPLQQVHKPMQHSLSGSDEPTLGG
                     GGETAKLSPGPEATGSATTIVPPQESPKFEPAQASADPPDRVRYERVLGQFRDGDLEG
                     ARQGFTGFLEDYPNSNLAPNARFWLGESYFGKKEFQRAIDAYDKVEIDYPGSEKIPAA
                     ILKKGYAYLALKDRKRASSAFKQVVTLYPKTTEAGKASDKLAQLKEVR"
     gene            1329..2087
                     /gene="ybgF"
                     /locus_tag="VN647_10785"
     CDS             1329..2087
                     /gene="ybgF"
                     /locus_tag="VN647_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HWG97559.1"
                     /translation="MCVRPLVSGSAAWGLLASTVVFAGCAKHADFLEMRNQLSTISRT
                     QDQDHQRVDAAMRRLEAVERSKDTDSGKPRFDDLAARFQKLESRLAKLEEAGQPSAKS
                     DASTEPRASKPIKQTQSAEPTVPVSGITPTSAFNLAYNDYLNGKYELAVAGFQRFIKD
                     FPGTSLTPNAQYWLGESYYNLKDYGRAIQTFDYLVAEYPGNEKVPAALYKLGLATAET
                     GDFGRSRKNLKRVLEEFPSSDESKLAKNKLAEIR"
     gene            2151..3923
                     /gene="mutL"
                     /locus_tag="VN647_10790"
     CDS             2151..3923
                     /gene="mutL"
                     /locus_tag="VN647_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWG97560.1"
                     /translation="MLRTDGSGKICILPEEVIGRIAAGEVVERPAAVVKELLENSLDA
                     GSRSITIDVRDGGLALIRVSDDGEGMSREDAPRAFQRHATSKLQSDLDLWSIRTMGFR
                     GEALPSIAAVAHVRLMTATRSADVGTELEVVGGAIGRIAEAPPITGTRIEVAELFHNQ
                     PARKKFLKSTLTEFLHISRVVQQASLAWPSIHFRLTHNAEEIINYPSAPSDDDRIAQV
                     YRRTFLDQCLRIEASLPGVSVSGHIVNAIHAKTTRIPQELFVNRRPVRNAAISHAVGE
                     GYGSFLAKGSQPRFVLFLKIDPDRLDVNVHPTKREVRFSDNELIHQLVRRAVRQALSG
                     GKTDELGKTPFVESSVRRTTGSTVLALPTSESVEAESTTLQPSPETQLPLVSEAAEPY
                     VRVPSAEVTALGQINRSFLIAQVGGDLTVIDQHTAHERVLFERLYRAWTTRGIQAQPL
                     LIPDPVELSPPQAALLQRYQHNLEQLGMEIEPFGTSTVLIRAIPVGLGKIDPTTFLED
                     LMDDLVQWDSVSSVEARVRSVLASVACHGAVRAGRSMKPEEIKVLVEDWRAEGEITTC
                     PHGRRTSFRLSIVDLEKMFGRAGW"
     gene            4034..>4510
                     /gene="miaA"
                     /locus_tag="VN647_10795"
     CDS             4034..>4510
                     /gene="miaA"
                     /locus_tag="VN647_10795"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWG97561.1"
                     /translation="MTKLSDIICGHRPLVVLLGPTAVGKSRIATQVAKHLDTDVLTVD
                     SRQVYRGMDIGTDKPTAEERQGVPHRLIDLVAPSEAFNAGWYRRVAMVEIERLYNAGR
                     LPFVVGGTGLYIRTLVKGLCSAPQADPDVRADLRKLRDEGGRDGLYTELMRVDPETA"
BASE COUNT         1077 a         1163 c         1357 g          913 t
ORIGIN      
        1 tcagcagtcc gacctgaaaa aagccgaaaa agatcttcag caacagctgt cccagactcg
       61 agccaggcag agccaggaaa tttcgacctt acgcgaacat gaactgccgc agctgcgagg
      121 agagctggaa aaggctctgc accaggctcg agagctccag aacaaacagg aagattttaa
      181 gcatcggtcg gcccagctgg agcagcagac aaaaaagctg gaacagttgg cggcaaagct
      241 ggaaaccgac agcagtacac gttatttatg gatgcagaag agcttcgaga cgcaggatgc
      301 gaaggtgtcg gcccggctcg atgagttatc gaaggccatg gaggcgttga aaaaagaggt
      361 cattgacgtc gtccaacgca cgaacgaagg gttggcaaaa cgtgtcgatg tcaagctgga
      421 agggcatcaa aaggaattgg cggagcacca gaataaaatc gagcagatct ctcaaaagtt
      481 cactcaattc aatcagtcgc tgacggggtt tcgggaagcg ctgaccggtc tgaatgatcg
      541 tgtgggtgaa tatgagcaaa ccgccaagca tctcacctcg agaatcgatg tcgattcaaa
      601 aacgacgacg actcatgcgg aggatgtcaa tcgaagcgtg atttccatca cgaaagcgct
      661 tgaagctgtc gggaaaaaag tcactgttcg ccttgacgag caagacaatc gaatcgatgc
      721 cttggcgaaa actcttgaac aagtatcaaa taggccggct cctctgcaac aggtgcacaa
      781 accgatgcaa cactccctgt ctgggtcgga tgagcccaca ttgggtggag gaggggaaac
      841 ggcaaagttg tcccctgggc cggaggcgac gggaagtgcg acaacgatcg ttcctcctca
      901 agaatcgccg aaatttgaac cggctcaggc cagcgccgac cctcctgatc gggttcgtta
      961 tgagcgggtg ttaggccagt ttcgggacgg ggatttggaa ggcgctcggc aaggatttac
     1021 aggatttctc gaagactatc caaactcaaa cctcgcgccg aatgcacgct tttggctggg
     1081 ggagtcgtac ttcggcaaga aagaatttca gagagcgatc gatgcctacg ataaagtgga
     1141 gatcgactat cccggcagtg aaaagatacc tgctgcaatc ttaaagaaag gatatgcgta
     1201 cttggcgtta aaagatagga agcgagcgtc gtccgccttt aagcaggtgg tcacgctcta
     1261 cccgaaaact accgaggcgg gaaaagcttc ggataaactg gctcaactta aggaggtgcg
     1321 gtaacaacat gtgtgtccgt ccgctcgtgt ccggttctgc ggcatgggga ttgttggcgt
     1381 cgacggtcgt gttcgccggc tgtgccaagc atgccgattt tctggaaatg cgcaatcagc
     1441 tttcgaccat ctcgcgaacg caggaccagg atcatcaacg ggtggatgcc gcgatgcgcc
     1501 gattggaggc agtagagagg agtaaggata cggattctgg gaaaccgcga ttcgatgacc
     1561 tggcggcacg ctttcaaaaa cttgagagtc gattggcaaa gctggaagag gccggccagc
     1621 catccgccaa gtcggatgcc tcgaccgaac cgcgtgcatc caagccgatc aaacagacgc
     1681 aatctgcaga accgacggtg ccggtgtcag gaatcacgcc gacgtccgcc ttcaatctgg
     1741 catataatga ttatctcaac gggaaatacg aacttgctgt cgcggggttc caacggttca
     1801 tcaaagattt tcccggtacg tcgttgaccc ccaacgctca atattggctg ggagaatcgt
     1861 attacaactt gaaagactat gggcgggcca tccagacgtt cgactatctt gtggcggaat
     1921 atccagggaa cgaaaaggtt cccgctgcgc tgtataagct tggtttggcg acggccgaga
     1981 ccggcgattt cggcaggtcg agaaagaatc tgaagcgtgt acttgaagag ttcccctcat
     2041 cggacgagtc gaagttggca aagaataagt tggccgaaat ccgatgatcg tcgccgtggc
     2101 gcgacgcccc gccccatcac cgtctcttcg ctcatgagga gcatcgtgcc gtgctgagaa
     2161 ccgacggcag tggtaagatc tgtattcttc cggaagaggt catcggtcgt attgcagcgg
     2221 gagaggtcgt tgaacgcccg gcggcggttg tcaaagagct cctcgagaac agtctcgatg
     2281 cggggagtcg gtccatcacg atcgacgtga gggacggggg gctcgccttg attcgggtga
     2341 gcgacgacgg agagggcatg agccgggagg atgccccgcg cgcgtttcaa cggcatgcca
     2401 cgagcaagct gcagtccgat ctggatctct ggtccatccg aacgatgggt tttcggggcg
     2461 aagcgctgcc gagcatcgcc gctgtcgccc atgttcgact gatgacggcg actcgttccg
     2521 ccgacgtggg gaccgagctg gaagttgtgg ggggagcgat cgggagaatc gccgaagctc
     2581 ctccgatcac aggaacacgc attgaagttg cagagctgtt ccacaatcag cccgcccgga
     2641 agaagttcct gaagtcgacc ctcacggagt tcttgcatat cagccgggtc gtacagcagg
     2701 cgtcgctggc ctggccatcc atccatttcc gtttgacgca caatgctgaa gagatcatca
     2761 attacccgtc tgcgccatcg gatgatgatc ggatcgccca ggtctatcgg cgcaccttcc
     2821 tcgaccagtg cctccggatc gaggcctcgc ttcctggagt ctctgtcagc gggcacatcg
     2881 tgaatgcgat tcatgccaag accactcgca taccgcaaga gttattcgtg aaccgtcgtc
     2941 ctgtgcgcaa tgccgcaatc tctcatgctg tcggggaagg gtacggttca ttcctcgcca
     3001 aggggagcca accgcgattc gtgctgtttc tgaaaattga cccggaccgc ctcgatgtca
     3061 atgtccatcc gaccaagcgg gaagtgaggt tttcggacaa tgagttgatc caccaactcg
     3121 tacggcgagc ggtccgtcaa gccttgagtg gcggaaagac ggatgagctt gggaagactc
     3181 cgttcgtaga gtcctccgtg cgacggacca cgggttccac cgtgctagct ctccccacat
     3241 ccgaatccgt tgaagcggaa tcgacgaccc tgcagccaag ccccgaaact caattgccgt
     3301 tggtgagcga agcggcggag ccttacgttc gagtgccttc agccgaggtg accgcgctgg
     3361 ggcagatcaa tcggtcattc ctcatcgctc aagtcggtgg cgatttgacg gtgattgatc
     3421 aacacacggc ccacgagcgg gtcctgtttg agcgactgta tcgcgcatgg acaacccgtg
     3481 gtatccaggc ccagccattg ctgatccccg atccggtgga actgtcgcca cctcaggcgg
     3541 cattgcttca acgctaccaa cataatctgg agcaattggg gatggagatc gaaccatttg
     3601 gtacctcaac cgtcttgatc agggcgattc cggtcggtct gggcaagatc gatcctacga
     3661 cgtttctgga ggatctcatg gacgatcttg tgcagtggga cagcgtctct agtgtggagg
     3721 cacgagtgag atcggtcctg gcatcggtgg cgtgccacgg cgcggttcgg gccggtcgct
     3781 cgatgaaacc ggaagaaatc aaagttctgg ttgaggactg gcgagccgaa ggggagatca
     3841 cgacctgtcc acatggacgg agaacatcgt tccgactcag catcgtagac ttggagaaaa
     3901 tgttcggacg agctggctgg tagcgagcaa ggtcaaggtt taggctgaga tcatattcga
     3961 tcagattcga atggctttct tcacgctctc ctcagtcgca ctctttcaaa aggcacatca
     4021 tcgctgatgg aatatgacaa agttatcgga catcatatgc ggacaccgtc ccctagtcgt
     4081 gcttctcggt ccgacggcag tcggtaagag tcgaattgca acacaagtgg ccaaacatct
     4141 tgacacagat gtgctgacgg tggattcccg tcaagtgtat cgcgggatgg acattggaac
     4201 ggataagccg accgccgagg agcgtcaggg ggtgccgcac cgacttatcg atttggtcgc
     4261 ccccagcgaa gcgttcaacg ccggttggta ccggcgggtc gcgatggtag aaatcgaacg
     4321 gttatacaat gccgggcggt tgcccttcgt ggtgggtgga acggggttgt atatccgaac
     4381 cttagtgaag gggttatgtt cggcgcctca agccgatccg gatgtgaggg cagaccttag
     4441 gaaattgaga gatgagggag ggcgagacgg tctctataca gagttgatgc gtgtcgaccc
     4501 tgaaacggca
//
LOCUS       DATNDD010000447         4696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3655539, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000447 DATNDD010000000
VERSION     DATNDD010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655539"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VN647_10800"
     CDS             complement(<1..1214)
                     /locus_tag="VN647_10800"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWG97562.1"
                     /translation="MSRLRLALVIVLAIVVLAVAFLSFSRELTGQDYLKDFVLEQLEE
                     SLGRKIDVHRVKFVIFPRIRVELTDVKIHDRESEQVVLTAKRVDLVLRLLPLLKKQVV
                     GKRLLIEEPTLTLRRNERGRWNVLEGLNGQADSDQPTMDMMARTFMISEATLVNGTIT
                     VIDAARPDGIRSLELEHVEFRLLVRPERSLAELHLSAAHQGESGVSAVSLDGAIKRAG
                     PSLSLSGEETTESTTGFQFEGQIDAADLKIRDVADFLGPRPVSKHLQGALNLKSTVRV
                     MPGVAGYDMVLSDMTAHLNNIMLKGKANLAGLLTPQPTFTVTFSSSLVALSQLLKTIP
                     PDWIHPQLPALLVDRQIDGKVQVVNATLTGTATTGPQLSTIGEFHISEGRGLIGSDRL
                     AAKDLAAVMVVET"
     gene            1440..3086
                     /gene="pgm"
                     /locus_tag="VN647_10805"
     CDS             1440..3086
                     /gene="pgm"
                     /locus_tag="VN647_10805"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HWG97563.1"
                     /translation="MAIHSLAGRPAPHSLLVDVEKLLSAYWTEKPDASAREQLVSFGT
                     SGHRGSSFRRSFNENHIVAIAQAVCEYRTTQQTTGPLYLGKDTHALSEPAFVTTLEVL
                     AANGVDIMIDRNGGYTPTPVISHAILTYNRGRTSGLADGIVITPSHNPPEDGGIKYNP
                     SSGGPADTQITKWIEDRANALLGANLQGVERLPIEQARRASTTHQHDYIGAYVNDLNN
                     VVDMNAIKSAKLKLGIDPLGGSGVAYWQPIVEHYGLNLEIVNPAVDPTFRFMPLDWDG
                     KIRMDCSSSYAMANLIALKDRFDVAFGNDADNDRHGIVTRSGLMNPNHYLAVSIAYLF
                     ANRPGWKSDAGIGKTLVSSSLIDRVAAKLNRKLVEVPVGFKWFVSGLLDGSLGFGGEE
                     SAGASFLRQDGTVWSTDKDGIIMDLLAAEMMAKTGRDPSELYRDLTKELGEPVYERID
                     APATPEQKTILSKLSPEQVNATVLAGDRIVAMLTKAPGNAAAIGGLKVVTKNGWFAAR
                     PSGTEDVYKLYAESFKGNKHLKRIQEEAQALVAGALGSST"
     gene            3100..3897
                     /locus_tag="VN647_10810"
     CDS             3100..3897
                     /locus_tag="VN647_10810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWG97564.1"
                     /translation="MNRHALRWPLPKHDYWSTPFAESLLQHLDLRPGLQTLDVASGHG
                     IPAFYLAEEVGPTGEVLAIDISAGQVARARAIQGSQLPWLRFECMDMRALPADLPTFD
                     RVTGNLSVMFFRPNRFETVRGLVEQLNPGGQLVLTFPSYGTFDSLWQRVDHAMIQQGL
                     ITERDRFQAYLNERPSAQEGRTWLERLNLERVEAIEYPLEVETGPPQEFLHHPLLRGG
                     FLDDVYECFEDQSLAERFMIEISQDVAQFTPLIAQRCVLSGWKRTSS"
     gene            4065..4355
                     /locus_tag="VN647_10815"
     CDS             4065..4355
                     /locus_tag="VN647_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97565.1"
                     /translation="MKKTSQSVSTTLTQGKLSLRAEMDRRNQQSTFEIYRRLDLGPGG
                     YAIAPSTNTRHGVRLALRENLADDPGRYRAVDESFALGAHCAADGLDDPLAT"
     gene            complement(4313..>4696)
                     /locus_tag="VN647_10820"
     CDS             complement(4313..>4696)
                     /locus_tag="VN647_10820"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWG97566.1"
                     /translation="QLYSYYVFNIGVAYRENVDEVMALLKDIAEGLRRDAEFAGDILE
                     PLEMSGVDRFADSAVIIKCRIKTKPIQQWRIGREMNRRIKNTFDAKNIEMPFPHQTIY
                     WGTPKDGTPSPLYVASGSSNPSAAQ"
BASE COUNT         1080 a         1318 c         1247 g         1051 t
ORIGIN      
        1 gtctcaacca ccatcactgc agcgaggtct tttgcggcta gacgatcact gccgatcaag
       61 ccccgacctt cgctgatgtg aaactcccct atcgtcgaga gttgaggacc agtcgtcgca
      121 gtcccggtga gcgtcgcatt caccacttgc accttgccgt cgatctggcg atccaccagc
      181 agagcgggaa gctgcggatg gatccagtca ggggggatgg tttttaacag ttgagagagg
      241 gcaacgagag aactgctgaa tgtgacggta aacgtcggct gcggagtcag gagaccggcg
      301 agattggcct ttcctttcag cataatattg tttaaatgcg cagtcatgtc ggacaggacc
      361 atgtcatatc cggcaacgcc cggcatgaca cggacggtgc tcttcagatt taacgctcct
      421 tgaagatgct tagaaaccgg cctgggaccg agaaagtcag ctacgtcccg tattttcaga
      481 tcagcggcgt cgatctgtcc ttcaaactga aatcctgtgg tcgattcggt tgtttcttca
      541 ccggacaacg acaacgatgg gcctgctcgt ttgatggcac catccagcga cacggcagat
      601 acgcccgatt ctccttgatg agccgccgac agatgcaatt ctgcgaggct acgttcaggc
      661 ctgaccagta gtctgaactc cacgtgttct aactcgagag atcgaatacc gtctggcctg
      721 gcggcatcaa tgaccgtgat cgttccatta acgagtgtcg cctcgctgat catgaatgtc
      781 cgcgccatca tgtccatggt tggctggtcg gaatccgcct gaccattcaa cccctccagg
      841 acattccaac gaccgcgttc gttccgccga agtgtcagtg tcggttcctc aatcagcagt
      901 cgtttgccca caacttgctt tttgagaagc ggcaagaggc gcaagaccag atcgacccgc
      961 ttagccgtca agacaacctg ctctgattca cgatcgtgaa tcttgacatc ggtgagttcc
     1021 acgcgaatac gggggaagat cacaaacttg acgcgatgca cgtcgatctt tctacccagg
     1081 ctctcctcaa gctgttcgag gacaaagtct ttgagatagt cttggccggt cagttctctt
     1141 gaaaatgaga ggaacgcgac ggccaatacc acgatggcga gtacgatcac gagcgcaaga
     1201 cgaagacgag acacatcatc cccttcaaat tacataccgg tccttcgtgg catcttaccg
     1261 gatcaatcac tcgaaatcca tgaacccagc gttctataaa ggtttttcta cgatttctat
     1321 agtttgacgg cctagaagac gcaaaggagc ctccaaagta accaccatat tttgaaaccg
     1381 gataaggggt tggccctttg tagttgacag gatcctgacg ggcgaactac atctctactg
     1441 tggctattca tagtcttgcc ggccgaccag cacctcactc cctcctcgtg gatgtggaaa
     1501 aacttttgtc cgcttactgg actgagaagc cggatgcctc ggcccgagaa cagctcgtgt
     1561 cattcggcac atcaggacat cgtggctctt ccttcagacg aagcttcaat gagaaccata
     1621 tcgtggcgat cgctcaagca gtttgcgaat accgaactac acagcagacg accggacccc
     1681 tttatttggg gaaagataca catgctctat cagagcctgc cttcgtgacg accctggaag
     1741 tcctcgcagc caacggcgtc gacatcatga tcgaccggaa tggtggatac acgccgacac
     1801 cagtcatctc tcatgccatc ctgacttaca atcgaggccg aacctccggt ctggccgacg
     1861 ggattgtcat cacgccgtcc cacaaccctc cggaagacgg cggcatcaag tacaacccgt
     1921 cgagcggtgg ccctgccgat acacagatca cgaaatggat cgaagatcga gccaatgccc
     1981 ttctaggcgc caatctccaa ggagtggaac gtcttccgat tgaacaggct cgcagggcat
     2041 cgaccacaca tcagcatgac tacattggag cctacgtgaa cgatctgaac aatgtggtcg
     2101 acatgaatgc catcaagtct gcgaaactga agcttgggat cgaccccctc ggtgggtccg
     2161 gtgttgccta ctggcaaccg attgttgagc attacgggct caatcttgag attgtgaatc
     2221 cagcggtcga tccaacgttc cgcttcatgc ccttggattg ggacggcaag attcggatgg
     2281 actgctcttc ttcctatgcc atggcgaacc tgatcgcctt gaaagaccgc ttcgatgtgg
     2341 cattcggtaa tgacgcggac aacgaccgac acgggatcgt cactcgttcc ggcttgatga
     2401 atccgaacca ttacctggcc gtctcgattg cctacctctt tgccaaccgt cccggttgga
     2461 agtccgacgc gggcatcggc aagacattgg tcagcagcag tctcatcgac cgagtggcgg
     2521 ccaaactgaa ccgaaagctg gttgaagtgc cggtcggctt caagtggttt gtcagcggcc
     2581 tgctcgatgg ctcacttggt ttcggcggcg aggaaagtgc cggcgcctca ttcttaaggc
     2641 aggatggcac agtttggtcc accgataagg acggcatcat catggatctg ctggccgctg
     2701 agatgatggc caagaccggc cgcgatccat ccgagttgta ccgagacctc acgaaagaat
     2761 taggcgagcc ggtttatgaa cggatcgacg cacctgccac tcctgaacag aaaaccattc
     2821 tctccaaact ttctcctgag caagtgaacg caacagtact ggctggggac aggattgtcg
     2881 cgatgctcac gaaggccccc ggaaacgccg cagccatcgg tggcttgaag gtggtgacca
     2941 agaacggctg gttcgcggca agaccgtccg gaaccgagga tgtatataag ctctacgcgg
     3001 aaagcttcaa agggaacaag cacttgaagc ggattcaaga ggaggcacag gccctcgtcg
     3061 ccggcgcatt gggttcttcc acatgatctg catggcacta tgaacaggca cgctctccgc
     3121 tggcctctgc cgaaacacga ttattggtcc acgccgttcg ctgaatcgct gctgcagcac
     3181 ctagaccttc gtccaggcct gcagactctc gatgtcgctt ccggccacgg cattccggcg
     3241 ttctatctcg ctgaggaggt tggtcctacc ggtgaagtgc tggccatcga tataagtgcc
     3301 ggtcaggtcg cccgtgcgag agccattcaa ggttcgcaat taccatggct tcgcttcgaa
     3361 tgtatggaca tgcgtgcctt gccggctgat ctgccgacct tcgatcgtgt cacaggcaat
     3421 ctctcggtca tgttctttcg tcctaacagg tttgagaccg tccgtggact cgtcgaacaa
     3481 ctcaaccccg gcggtcagct cgtcctgacc tttccatcct atggaacatt cgattcacta
     3541 tggcagcgag tagaccatgc gatgattcag caaggactga tcaccgagcg agaccgattc
     3601 caggcctatc tgaatgaacg gccatcggcg caggaaggac gaacgtggct tgaaagactc
     3661 aatcttgaac gggtcgaagc catcgagtat ccgcttgaag ttgagactgg tcccccccaa
     3721 gagtttcttc atcaccctct cctccgtgga ggttttctcg acgacgtgta cgaatgtttt
     3781 gaagatcagt cgcttgctga acggttcatg atcgaaatct cacaagacgt tgctcaattt
     3841 actccgctga tcgcgcaacg ctgtgtgctc tcagggtgga aacgaacttc gtcttgaggt
     3901 catgatgttc ggcataacaa gaccggtaaa ctccaagtta tagagattga gttgacggcg
     3961 agaggttgat gcgtaacagt ctgtatgacg tttgcgccaa gaagtaattt cctggacctc
     4021 gcaaccatta ctgggagtta gagtctgaaa caggtccgag ggacatgaag aagacaagcc
     4081 agtccgtgtc caccacgctc acgcagggga aattgtcgtt acgagccgag atggaccgtc
     4141 gtaatcagca atctacattc gaaatttacc gccgccttga tcttgggccg ggtggatatg
     4201 cgattgcgcc ttcgaccaac acccgacacg gcgtacggct cgcgttacgc gaaaatctcg
     4261 ccgatgatcc tggtcgatac cgggccgtgg atgagagctt cgcgcttggc gctcattgcg
     4321 cagccgacgg attggacgac ccgctcgcca catagagcgg cgacggagtt ccatccttgg
     4381 gtgtgcccca gtagatcgtc tggtgcggaa acggcatctc gatgtttttg gcatcgaagg
     4441 tgttcttgat ccgacggttc atctcacggc cgatccgcca ttgctgaatc ggttttgtct
     4501 tgattcggca cttgatgatg accgcagaat cggcaaaccg atcgactccc gacatctcca
     4561 gcggctccaa gatgtcgccg gcaaactccg catctcgccg caatccttcg gcaatgtcct
     4621 tcagcaaggc cataacctca tcgacgtttt cccggtaggc gacgccgata ttgaagacat
     4681 agtaggaata gagttg
//
LOCUS       DATNDD010000448         7020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_4102691, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000448 DATNDD010000000
VERSION     DATNDD010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4102691"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VN647_10825"
     CDS             <1..856
                     /locus_tag="VN647_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HWG97567.1"
                     /translation="WEKRYGNVLVTYNERGLRDRPILPKAEGEYRILALGDSVTFGLG
                     VDQDKTFAARLELLLQDRLHRPVRVINSGVGGYNTVQELSYFKQEGIDLQPDLVLLTY
                     VTNDIEEKRKPFDPWSKSSMRGKPFPVMVEIMMEKLWLYRLAHHTYRYAVLEQLNEKS
                     LARSQNGTGWRGSMSALSELVAICQERKIPLILFFKRLHPDEHNLLFDDVVLNAQGLP
                     VKDMAPWFARLNVSSFVNSKVDTHPNAEGHRVIAEHMADDVVNYFAQSKDRASLIWSG
                     IRVAAVDP"
     gene            complement(1005..1304)
                     /locus_tag="VN647_10830"
     CDS             complement(1005..1304)
                     /locus_tag="VN647_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97568.1"
                     /translation="MNRTDKVALITPWINPEERITVQFDDQRDINTIVTGCTERLIDL
                     ELETNVPYLRQKLSIPLSDVDVSEDSSHYTRDPDKPLQFKRLLLIVNGKRPPIVY"
     gene            complement(1381..1752)
                     /locus_tag="VN647_10835"
     CDS             complement(1381..1752)
                     /locus_tag="VN647_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97569.1"
                     /translation="MGLTGKLMIAVLGISIWLAIPSYAQLGGGRQDAADILNGMPPDV
                     LAKVRSLAQVLQQGIKDGKISDAEIQQGLTSGQLGEKLKQLSPEAAQLLEEISDASRQ
                     GKGPGEESLMPLMGGLGISPN"
     gene            2000..2770
                     /locus_tag="VN647_10840"
     CDS             2000..2770
                     /locus_tag="VN647_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97570.1"
                     /translation="MLEGWESPDVTVYLAAILGLLVVWQYYQMQIMAGRILAIDIFDR
                     SGIRMYIYVIPSDDHICEVCAASHGRFFLPSQVAKKQFSPLTGKCQRPVPPCIGVLVG
                     LYGAWLEARGVLEHLRKNSQKGGIQLSPEEVRVLVNGQWERCISADTDRLGIHMIEAL
                     SYEKINPEVSIEAYRYVVNEVKEVRHLMFLVPAYLRLMQLLLRAGEEAEALETIERFA
                     SRFPSTKRGRHFPSDEQRETMKTKKAKLLKNLPLQMSA"
     gene            complement(2876..4294)
                     /locus_tag="VN647_10845"
     CDS             complement(2876..4294)
                     /locus_tag="VN647_10845"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWG97571.1"
                     /translation="MESADRQNAPYDGSALIADPIHKYVTFTVPYAHPDPHEHTEKEL
                     IDSPWVQRLRYIYQLQSARWVYPSAEHTRFVHSLGTMHVAGRFARHLYPFLKKAVKDV
                     PSANFVEELLRVTALVHDIGHGPFCHFFDDNFLHAHGLTHERLGQIIIREHLAPLIRK
                     IRRSPSGPFAKGEELNPDQIAHLILKEKGKDNSRLPRWLNMLQPVISGSYTGDNLDYV
                     LRDSYMCGVAVGPVDLTRLIHYTIVTEKGFTIHKTGLPSLQMFLNTRMYLYSNVYFHR
                     TTRAIDIHLRDIFGQTMQLLCPNDPRKNMERYRTLTDWSLLEEVRGWKQSRHKARRQL
                     GQEWARILGRDVKWKMAYSTTLKEKGQERGMTFPSHQHFEQQIAKELPSGLRRLPFRV
                     DMALLDPRPDPKDRRGHPLYVYDPGTGQVSTEPLEEFLDLLPTRLIQFRIYSPDHQHD
                     AALSQAAATVLNKTPTSLESNY"
     gene            complement(4309..4824)
                     /locus_tag="VN647_10850"
     CDS             complement(4309..4824)
                     /locus_tag="VN647_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HWG97572.1"
                     /translation="MSDMNHQPLTRGARSFRALLRQMLHLQESPQRTALAFALGVFIA
                     FSPAYGLHTAMVVLCTWLFGLNFLALFTGALVNNPWTIIPILGATYWTGALLLGRTDM
                     PNFDWQDLSVSGIYEQVLPYAAPFMLGGLVLSVLGALLSYPAAYLFFHKHRSSPAKPT
                     TEPLPPSDQVG"
     gene            complement(4824..5867)
                     /gene="thiL"
                     /locus_tag="VN647_10855"
     CDS             complement(4824..5867)
                     /gene="thiL"
                     /locus_tag="VN647_10855"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWG97573.1"
                     /translation="MAPRPANAPLQEFALIRSLEGRFARHAQGLVQGIGDDAAVIETS
                     AKTWWHLTTDLLAEGIHFNLKSVTPESVGYRAAMANLSDIAAMGAVPRYLLISLAVPR
                     IVKRSHIDELYTGLMKACRFYDVALIGGDTSASKAGLFLSITLVGTTSGRRALFRHGA
                     EAGDHIYVSGTLGDSLAGLKLLTNSTTSQPSEKRKSEFSRSHRRFLVRRHSHPTARVA
                     EGRWLNERQLASAAIDLSDGLSGDLRHLCEASRVGAEVELEKLPISPACRAYARTIGI
                     SPIRLALTGGEDYELLFTASSRDSNTIDRQAETRGYRMTRIGTIRPHRFGIQMTSDGR
                     TQPMPATSYEHFR"
     gene            complement(5877..>7020)
                     /locus_tag="VN647_10860"
     CDS             complement(5877..>7020)
                     /locus_tag="VN647_10860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HWG97574.1"
                     /translation="LEVLDPEQNSAFTDHYLGVPFDLTEVMFITTANLIDPILPALRD
                     RMEVIEIPGYTEEEKLGIAQKYLIPRQLEEHGITSKHVRLTEPAIRQIISHYTREAGV
                     RNLEREIANVMRKVAKKVAEGKGLGDPIDPSNLHKYLGVSKYVPEAELEKDEIGVATG
                     LAWTEAGGDVLYIEATVMKGKGQLTLTGHLGDVMKESAQAALSYVRSREKTLHLSPAM
                     FSKQDLHIHVPAGAIPKDGPSAGITMATAIASAFSGTPARRDLAMTGEITLRGRVLPI
                     RGLKEKILAAKRAKLTTVILPRRNKKDLEEIPKHLLKGIQLAFADTMDDVMKIALRRS
                     AKTSPASRKPSTPPAPTRIRPLRPGKGRRSHELPATVMANRAPTRS"
BASE COUNT         1498 a         1805 c         2087 g         1630 t
ORIGIN      
        1 atgggaaaaa cgctatggta acgtgctcgt gacttataac gaacggggct tgcgcgatcg
       61 acctattttg cccaaagcag agggtgaata caggattctc gcactcggag actcagtgac
      121 ttttgggttg ggggtggacc aggacaagac gtttgccgcc cgcctcgaat tgttactgca
      181 ggaccgtctg caccggccgg tgcgcgtgat caacagcggg gttgggggct acaatacggt
      241 ccaggagttg agctatttca agcaagaagg gatcgacctg cagccggatc tggtgctgtt
      301 gacttatgtg acgaatgata tagaagagaa aagaaaacct tttgatccgt ggagtaaaag
      361 ctctatgcgt gggaagccgt tcccggtcat ggtagaaatt atgatggaga aactgtggct
      421 gtatcgattg gcgcatcata cttatcgcta tgccgtactc gaacagctga acgagaagtc
      481 tttggcccgc tcacaaaacg ggacggggtg gcgtgggtcc atgtcagcgt taagcgaatt
      541 ggttgcgatt tgtcaagaac gcaagatccc cctcatattg ttctttaagc ggttgcaccc
      601 ggacgagcat aatctgttat ttgacgatgt ggttctgaat gcccagggcc tcccggtcaa
      661 ggacatggca ccatggtttg cgaggcttaa cgtatcctca ttcgtgaatt caaaagtcga
      721 tacccaccct aatgcggaag gccatcgagt gatcgctgag cacatggccg acgacgttgt
      781 gaactacttt gcccagtcaa aagatcgggc aagtctgatc tggtcgggca ttcgtgtcgc
      841 tgctgtcgac ccatgaattg atcgacgttg ccaaagacag tttccgtcca atgaataata
      901 gggccggcat tcggcttgtc tgtcaatggt gttgaggtag gggacactag ccaatgggtc
      961 gctttagtgg tctggagatg acccgagaat cgcgcatggc tctcctaata cacaattggc
     1021 ggccgtttgc cgttgacgat caacagcagc cgcttaaatt gtaacggctt gtcgggatca
     1081 cgcgtatagt ggctggaatc ttctgagacg tcgacatcgc tcagcgggat cgacagtttc
     1141 tgccggagat aggggacgtt ggtttccaat tccagatcga tgaggcgttc ggtgcagccg
     1201 gtcacgatcg tgttgatatc gcgttgatcg tcaaactgca cggtgatccg ttcttctggg
     1261 ttgatccatg gcgtgataag ggccaccttg tcagtgcgat tcatggcgga aggattgtcc
     1321 attgtgcttc tatagtactc ccataagttc tcgcgatgtc actcggatat tcccgaggag
     1381 ttaattcggt gagatgccca atcctcccat caacggcatc aagctttctt cccctggtcc
     1441 ctttccctgc ctcgatgcat cactgatctc ttcaaggagc tgagcggcct cggggctcag
     1501 ctgtttcaac ttttctccaa gttgtcctga cgtcaggcct tgttggatct cggcatccga
     1561 gattttcccg tctttgatgc cctgttggag aacctgtgct aaggaccgca cctttgccag
     1621 gacgtcgggg ggcatgccgt tcaggatatc agccgcgtcc tgtcggccac ctccgagttg
     1681 ggcataggat ggaattgcca accaaataga gatccccaaa acagcgatca tcagttttcc
     1741 tgtcaaaccc atcgcagctt ccctcctctc ttttggcatc atgagtcgag cctccgatca
     1801 agccgaatcc tgcaggatgt ttgccagttc tccatgagaa aggaaaggat gtcttagaga
     1861 gaagaactag tgtcaagtgc atgcaggtat taaccgatac agttccatac ccatttgaga
     1921 aaacagagat tttttagacg acctgtctga gaattggtat ggtttatctt catgagaccg
     1981 agagtggagg ggagcgctga tgcttgaagg gtgggaatct cctgacgtca cggtctattt
     2041 ggcggccatt cttggacttc tcgtcgtgtg gcaatactat cagatgcaaa tcatggccgg
     2101 gcgcatcctg gccatcgata tcttcgatcg ttccggcatt cggatgtata tctatgtgat
     2161 tcccagcgac gaccatattt gtgaggtctg tgccgcatcg catggccgct tcttcttacc
     2221 ttcccaggtg gcgaagaaac agttttcacc gcttaccggg aagtgtcaac ggccggtgcc
     2281 gccctgtatc ggagtattgg tcggactgta tggtgcctgg ttggaagcga gaggcgtgtt
     2341 ggaacacctc cgtaaaaatt cccaaaaggg cggcattcag ttgtctccgg aagaagtgcg
     2401 ggttttggtg aacgggcagt gggaacggtg catcagcgcc gacacggatc gcctggggat
     2461 ccatatgata gaagccctca gctatgaaaa gattaatcca gaggtctcaa tcgaagccta
     2521 tcggtatgtg gtcaacgagg tgaaagaagt gcggcatctg atgtttcttg ttcccgccta
     2581 cctccgactc atgcagttgt tgcttcgagc cggagaggag gctgaagcgc ttgagactat
     2641 cgagcgcttc gcaagccgct tcccatcgac gaaacgcggt cgccatttcc catcggatga
     2701 gcagcgggag accatgaaga cgaaaaaggc caaactgctg aaaaacctgc ctctccaaat
     2761 gtctgcctaa gaaccaccaa gggaccacga ctgtctgtcc tccgcagtag tctgctacgg
     2821 aggatggatc aagagcggtg gggctccacg ctacgacgat cgactcacca atcgttcaat
     2881 agttggattc caggctcgtc ggtgttttgt tgaggacggt cgctgcagcc tgcgacaggg
     2941 ctgcgtcgtg ctggtggtcg ggagaataga tccgaaactg gatgagtctc gtgggcagca
     3001 ggtctaagaa ttcttccaac ggctcggtcg aaacctgacc cgtcccggga tcatacacgt
     3061 aaagaggatg tccacggcgg tctttggggt caggtcgtgg atctaacagg gccatgtcca
     3121 cgcgaaacgg caggcgcctc aagccggagg gcagctcttt ggcaatctgc tgttcaaaat
     3181 gctgatggct ggggaacgtc atgccgcgct cctgtccttt ttctttcaac gtcgtgctgt
     3241 aggccatttt ccatttgacg tctcggccta agatccgtgc ccactcctgt cctaactgac
     3301 ggcgggcttt gtggcgggac tgcttccatc ctcggacttc ttccaagagc gaccaatcgg
     3361 tcagggtccg atacctctcc atattcttgc gcggatcgtt cggacagagg agctgcatcg
     3421 tctgaccgaa gatatcccgt agatggatgt cgatggctct ggtggtgcga tggaaataga
     3481 cattggagta gaggtacatc ctggtgttca gaaacatttg gagggacgga aggccggttt
     3541 tgtgaatagt gaatcctttt tccgtcacga tcgtgtaatg aatcaacctc gtcagatcga
     3601 ccgggccgac cgccacgccg cacatatagg aatcccgcag gacatagtcc aaattatcgc
     3661 cggtgtaact ccctgagatc acgggctgaa gcatgttcaa ccaacgaggg aggcgggaat
     3721 tgtccttgcc tttctccttg aggatcagat gggcgatctg gtcgggattc aattcttccc
     3781 ctttcgcgaa gggtccggag gggcttcggc ggattttccg gatgagagga gccaaatgtt
     3841 cccggatgat gatctggccc aaccgttcat gcgtgaggcc atgggcatgg agaaaattgt
     3901 catcgaagaa atggcaaaat ggaccgtgcc cgatgtcatg aaccaaggcc gtcacccgca
     3961 agagttcttc gacaaaattg gccgacggca cgtctttgac ggccttcttg aggaatggat
     4021 acagatgtcg ggcgaatcgt ccagccacgt gcatcgtccc gagagaatgc acgaatcggg
     4081 tatgttccgc cgagggatag acccagcggg cactctgcag ctggtagatg tatcgcagcc
     4141 gctgaaccca gggagaatcg atcagctctt tctcggtgtg ttcatgggga tcaggatgag
     4201 catagggtac ggtgaaggtg acgtacttgt gaatgggatc ggcgatgagg gctgatccat
     4261 catacggggc attctgtcgg tctgccgatt ccatggcgag tgggcatctt agcccacctg
     4321 gtcggaaggc ggcaacggtt cggtcgtagg tttggcaggc gaggaacgat gtttgtggaa
     4381 aaacaggtag gcggccggat aggacaacaa ggccccgagc acactgagaa cgagtcctcc
     4441 aagcatgaag ggggcggcgt agggaaggac ttgttcgtat atgccgctga cactcagatc
     4501 ctgccaatca aaattcggca tatcggtccg tcccaagagc aatgcaccgg tccagtaggt
     4561 tgcgcctaag atcggaatga tggtccaggg attgttgact aaggcaccag tgaacaaggc
     4621 caagaaattg agtccgaaca gccaggtgca cagcaccacc atcgccgtgt gcagaccata
     4681 ggctggtgag aacgcgatga agacgcccag cgcaaatgcc agcgcggtgc gttgcggtga
     4741 ctcctgtaag tggagcattt ggcgtaagag tgcccggaat gatcgggccc ctcgcgttaa
     4801 gggctggtga ttcatgtcgg acatcagcgg aaatgctcgt aactggtagc gggcatcggc
     4861 tgcgtccgac cgtcggacgt catctgaatc ccgaaccgat gcgggcgaat tgttccgata
     4921 cgggtcatgc gatagccgcg tgtttctgct tgccgatcaa tagtattgct gtcgcgcgaa
     4981 gaagcggtga acagaagttc atagtcttcg ccaccggtga gtgcgagtcg aatcggcgag
     5041 atgccgatcg tccgggcata ggctcgacag gccggtgaga tgggtagctt ttcaagttcc
     5101 acctctgcgc cgactcgact tgcttcgcac aggtgccgaa gatcgccgga gaggccgtcg
     5161 gacagatcga tggcggcgga ggcgagttgc ctctcgttga gccaccgtcc ttcggcgacc
     5221 ctcgcggtag ggtgggaatg gcggcgaaca agaaatcgcc gatgggagcg tgagaattcg
     5281 ctcttcctct tttccgaggg ttgcgaggtt gtgctgttcg tcaagagttt gaggcctgcc
     5341 agggagtctc cgagagtacc cgagacatag atgtgatctc ccgcttcggc accgtgccga
     5401 aaaagcgccc gacgtccgct cgtggtaccg accagggtaa tgctgagaaa gagtccggcc
     5461 ttggacgccg aggtgtctcc accgatgagt gccacgtcat aaaagcggca agccttcatc
     5521 aagccggtgt acagctcatc gatgtgtgac cgcttcacta tcctcgggac ggcgagcgaa
     5581 atcagcagat agcgagggac ggcgcccatc gcggcaatgt cgctgaggtt ggccatggcg
     5641 gctcggtagc cgaccgactc aggcgttacc gatttcagat taaagtggat tccttcggca
     5701 agcaggtccg tggtgagatg ccaccaggtt ttggcggatg tctcgatcac ggctgcatcg
     5761 tcgccgatgc cttgaacgag cccttgcgca tgacgggcaa accgaccttc gagcgatcgg
     5821 atcagagcaa attcttgaag cggggcgttg gccggacgag gggccacggc actatcctat
     5881 gatcgtgtgg gcgctcggtt tgccatcacc gtggcgggaa gttcatgcga ccgtcggcct
     5941 ttgcctggtc gcagggggcg gatgcgcgtc ggggccggag gggtcgatgg tttcctagag
     6001 gcaggcgaag tcttggcgct tcggcggagg gcgatcttca tgacgtcgtc catagtatcg
     6061 gcgaacgcca attgaatgcc tttaaggaga tgtttgggaa tttcctccag gtccttctta
     6121 ttccgacgtg ggagaatcac cgtggtgagc ttcgcccgtt tcgccgccaa aatcttttcc
     6181 ttgagtcccc tgatcggaag cacgcggccg cgcaacgtga tctctccggt cattgccaag
     6241 tctcgcctcg cgggcgtacc ggaaaatgcc gaggcaatgg cggttgccat cgtgatgccg
     6301 gccgagggac catccttggg aatagccccg gccggcacat ggatgtgcag gtcttgtttg
     6361 ctgaacatcg ctgggctgag atgcaaggtt ttttctcgtg agcggacgta gctgagcgcc
     6421 gcctgagcgg attctttcat cacatcgccc aggtggccgg tgagggtcaa ttggcctttc
     6481 cctttcatca ccgtcgcctc gatgtagagc acatccccgc ccgcttccgt ccaggctagg
     6541 ccggtcgcca caccgatttc gtctttctcg agttccgctt ccggcacata tttcgacact
     6601 cccagatatt tatggagatt ggacggatcg atcggatctc ccagaccttt gccctcggcg
     6661 actttctttg caaccttacg catcacattg gcgatctcgc gctcaaggtt ccgcaccccg
     6721 gcctcccgcg tgtagtgcga aatgatctgg cggatcgccg gctccgtaag acggacatgt
     6781 ttgctcgtaa tcccgtgttc ttcgagctgg cgggggatca ggtatttctg ggcaatgccg
     6841 agcttctctt cttcggtata tccgggaatt tcgatgacct ccatgcgatc acgcagcgcg
     6901 ggaaggatcg gatcgatcaa attcgccgtg gtaatgaaca tcacttcggt caggtcgaat
     6961 gggacaccga gatagtggtc ggtgaacgca ctgttttgtt cagggtcgag gacctccagt
//
LOCUS       DATNDD010000449         5117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2479404, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000449 DATNDD010000000
VERSION     DATNDD010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479404"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /gene="accC"
                     /locus_tag="VN647_10865"
     CDS             <1..1243
                     /gene="accC"
                     /locus_tag="VN647_10865"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWG97575.1"
                     /translation="EADALALHVRAADEKVCVGPAESALSYRNIPNVLSAAEITGVDA
                     IHPGYGFLSENAHFAEVCESIGVKFIGPSSENIAMMGDKAKAREIVAKRGLPVTPGSP
                     GELRSEEDALQAAQKIGFPVIIKATAGGGGRGMRVVNKAEDLGRAFQAAQTEAKSTFG
                     NDGVYLERYFLEPRHIEVQILADQYGRVIHLGERDCSIQRRHQKLVEETPSPVVDDKL
                     RREIGRVAVEAVKAAHYRNVGTVEFLLDKDHQFYFMEVNTRIQVEHPITEMVTGIDLI
                     KEQIRLAAGHPLSIRQQDVILTGHSLECRINAEDPEKFTPSPGVITKYSPPGGFGIRV
                     DSAMESGSIVVPHYDSMIAKLITHGRDRQESAARMKRALGEFIIEGIKTTIPLHRRIL
                     DDPDFQKGHVSTTFLERFLTS"
     gene            1405..2685
                     /locus_tag="VN647_10870"
     CDS             1405..2685
                     /locus_tag="VN647_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWG97576.1"
                     /translation="MLDVSSAQHMRRLGFIISIVAIGIAIGGYVFFNGERKPPIRYRT
                     AAVERGQVVSVVSATGTINPVVSVQVGTQVSGMIKSLHADFNSRVKAGDTVAIIDPEP
                     FKARREQAAGNLEMARSNVARSKADLAQRKRELDRVQSLLPQQFVSQNDVDVALTNFQ
                     SAEAQLRVAGAQVKQAEAALNAAELELKYTVIRSPVDGIVVARNIEVGQTVASSFATP
                     NLFLIALDLTNMQVDTNVSESDIGGMTEGKEAVFTVDAYPGVSFAGTIKQVRLAPINV
                     QNVVTYNVVISVDNKELRLKPGMTANVAIVVAQRDQVLKVPNAALRFMPPKAEGDRRG
                     LEGQATKAEGGRPIHAGGAAGQSRTVWTQMESGDLVSLSVQTGISDGIATEVLSGGLT
                     EDDSVVVGIEQPFGERKSSELPPGFGNQRRPRSR"
     gene            2800..3510
                     /locus_tag="VN647_10875"
     CDS             2800..3510
                     /locus_tag="VN647_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG97577.1"
                     /translation="MNALIICEDIWKVYRVGDVEVQALRGVNLTIRQGEFVAIMGSSG
                     SGKSTLMNILGCLDQPTKGQYRLNSIDVGHLRPDRLAEIRNQQIGFVFQSFNLIPRTS
                     ALENAQLPLFYRGLPLKEQKSLASAALQRVGLGGREHHSPTQLSGGQQQRVAIARALV
                     TSPSLLLADEPTGNLDTQSSQEIMEILEGLNREGITVILVTHEVDIAAYASREIVIKD
                     GQILSDRTTNERSQVVGA"
     gene            3511..4746
                     /locus_tag="VN647_10880"
     CDS             3511..4746
                     /locus_tag="VN647_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWG97578.1"
                     /translation="MPAFVWLTIVTALRILGRNRLRAGLTMLGIVIVVGAVIAMVSIG
                     EGAKQAVQTQIATMGTNVIMIWPSSTSVGGVRGAQGGAVTLTVADAVDLKKKIPVLSD
                     TAWMKRDVLQVVNGHLNGNSPVNGVSPSYLSIRDWTFSKGGPFTQADMDSVALVALLG
                     QSVVDEIFDPGEEPLGAIIRIRNVPLRVIGVLSPKGQSTYGQDQDDVVFIPFTTAERK
                     VFGTLFLGSVGGLYAATDRAEDMPEAVEQIREVMRSRHRLQADQPDDFTIRTQVELGK
                     VQEGTSQTLTVMLMAIASVSLLVGGIGIMNILLVSVTERTREIGVRMAVGAKRAHILI
                     QFLIEAMTLSVVGGCIGILLGVLSARLTTVIAGWPTIISGDTVATAFGFSIAVGLFFG
                     LYPANKAARLNPIDALRYE"
     gene            complement(4763..>5117)
                     /locus_tag="VN647_10885"
     CDS             complement(4763..>5117)
                     /locus_tag="VN647_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97579.1"
                     /translation="DRYDELITGFGGTSPLTPHQTFQCLYQEARHGKLDQRIVSSFIA
                     RVGIYPVHSHVRLNTQELAVVTELNQERLHLPIVTITHQPGGTEYVTPLVVDLAHQTD
                     ETRTRAIETIIAMDP"
BASE COUNT         1174 a         1288 c         1493 g         1162 t
ORIGIN      
        1 tgaagcggat gcgcttgcac tgcacgttcg agcggccgac gaaaaggtgt gtgtcgggcc
       61 ggccgaatcg gcattgagtt accgaaatat tcccaacgtc ttgagtgccg cagagattac
      121 gggagtcgat gccatccatc cagggtacgg atttttatcg gagaatgccc attttgctga
      181 agtctgtgag tcaatcggcg tgaagtttat cggaccgagc tcagaaaata tcgccatgat
      241 gggggataaa gcgaaggcgc gtgaaatcgt ggcgaagcga ggactcccgg tcacgcccgg
      301 gagtccaggc gaattgcgca gcgaagagga tgcgttgcaa gcggcgcaga agatcggctt
      361 tcccgtcatt atcaaagcca cggcaggtgg gggagggcgc ggcatgcgtg tcgtcaacaa
      421 ggccgaagac ctgggccgag cttttcaggc agctcagact gaagcgaaat cgaccttcgg
      481 caatgacggt gtatacctcg aacgatactt tctggaaccg cgccatatcg aggtgcagat
      541 tttggccgat caatacggtc gggtgatcca tcttggagaa cgagactgtt caattcaacg
      601 gcgacatcaa aaactggttg aagaaactcc ctcccctgtc gtcgatgaca aattgcgtcg
      661 ggaaatcggt cgcgtagctg tggaggcggt gaaggctgcg cattaccgaa atgtgggcac
      721 cgtagaattt ttactcgaca aagaccatca gttttatttc atggaagtca atacccgcat
      781 ccaggtggaa catccaatca ccgaaatggt gacgggcata gatctgatca aggaacagat
      841 ccgtttggcc gccggtcatc cgctttctat tcggcaacaa gatgtgatcc ttaccggaca
      901 cagcctggag tgtcgtatta atgccgaaga tcctgagaag tttacaccgt cccccggcgt
      961 gatcaccaaa tacagcccgc cgggagggtt cggaatccgt gtcgactccg cgatggagtc
     1021 aggttcgatc gtcgttcctc actatgattc gatgattgca aagctgatca cccatggccg
     1081 tgaccgacag gagtcagcgg ctcgtatgaa acgcgcgctt ggcgagttta tcatcgaggg
     1141 cattaagacc accattccgc ttcaccgtcg aatccttgac gaccccgatt ttcaaaaagg
     1201 ccacgtgtcg acgacgttcc tggaacggtt cttgacaagc taagcccgca agttctctct
     1261 ccgaaagaac agtcttttcg gtgtttctcc atcaagtgat ctcatttctt ggtaagcgta
     1321 gagtctcatg gtaagctcat caaagtgctc cccgcttcaa gcgggaaggt gcatcaccta
     1381 ttggtttcct gataggatac accggtgctc gatgtgtcga gcgcccaaca tatgcgacgt
     1441 ctcggcttta ttattagtat cgtggctatc ggaattgcga tcggtggcta cgtgtttttc
     1501 aacggagaac ggaagccccc cattcgatac cgaaccgcag cagtcgaacg tggtcaggtc
     1561 gtttctgtcg tcagcgctac aggcaccatc aatccggttg tatccgtcca ggtcggcacg
     1621 caagtgtcag ggatgatcaa aagtcttcac gccgatttca actcacgcgt gaaagccggg
     1681 gatacggttg cgatcatcga ccccgaaccg ttcaaggctc gtcgagaaca ggctgccggc
     1741 aatctggaaa tggcgcgatc gaacgttgca cggtccaaag ccgacctggc gcagcgaaaa
     1801 cgggagctcg accgtgttca atcgctgttg cctcagcaat ttgtttcaca aaacgacgtc
     1861 gatgttgcac tcacaaactt ccaaagcgcg gaagcacagt tgcgggtcgc tggggcgcag
     1921 gtcaagcaag cggaagcggc attgaatgcg gcggagctgg aattgaaata taccgtcatt
     1981 cggtcgcctg tggacggtat tgtcgttgcg cggaatatag aggtcggtca aacggtcgcc
     2041 tccagttttg ctacgcccaa cttatttctg attgccctcg atctgaccaa tatgcaagtc
     2101 gataccaacg tgagtgagtc ggatataggc gggatgacgg aagggaaaga agcggtgttc
     2161 acagtcgatg cctatccggg agtgtccttc gctggtacca ttaagcaggt gcgcctcgca
     2221 cccatcaatg tccaaaatgt cgttacctat aatgtggtga tcagcgtaga taataaagag
     2281 ttacgtctaa agcctggaat gactgcgaac gtagcgattg tggtggctca aagggaccag
     2341 gtgctcaaag ttcctaacgc ggctctacgc ttcatgcctc cgaaggctga aggagaccgc
     2401 cgaggattgg aaggacaagc gacaaaagcg gaggggggac gcccgatcca cgcaggagga
     2461 gcagcggggc agtcgaggac tgtttggaca cagatggaaa gcggtgacct cgtttccctg
     2521 tcggtccaaa cgggtatctc ggatggcatc gccaccgaag tcctgtccgg cggtctcacg
     2581 gaagatgatt cggtcgttgt cggtatcgag cagccttttg gggaaagaaa gagcagcgag
     2641 ctgccgccag gattcggcaa ccagcgtcgc cctcgttctc ggtgaaccat cggattgtgt
     2701 ggaacatacg gtgtatgcga tttggcttgc cgaacgtaat ctcttatcac tgtccgccgg
     2761 tcattcatct tcggataaca tctcttacag gataggtttg tgaacgcgct gatcatttgt
     2821 gaagatattt ggaaggtcta tcgagtcggc gatgttgaag tgcaggcgct gagaggggtg
     2881 aatctcacca tccggcaagg tgagttcgtt gcgattatgg gatcgagcgg atcaggcaaa
     2941 tccacattga tgaacattct cgggtgcctt gatcaaccga ccaagggaca gtatcggctg
     3001 aacagcattg atgtcggaca tttgcggcca gaccggttgg cggagatccg gaatcagcaa
     3061 atcggctttg tttttcaaag tttcaatctc attcctcgca ccagcgcgtt ggaaaatgcc
     3121 caattaccgc tgttttaccg agggcttccc ctcaaggaac agaaatctct tgcgtccgct
     3181 gcgttgcagc gtgtggggtt gggcgggcgc gagcatcatt caccaacaca actttccggg
     3241 gggcagcagc agcgagtggc gatcgctcgg gcactggtga catcgccctc gttgctcctt
     3301 gcggatgaac cgacgggaaa tctcgatacg cagtccagcc aagaaatcat ggagattctt
     3361 gaagggttga atcgggaagg aatcaccgtg atcctggtca cgcacgaagt cgatattgcc
     3421 gcctatgctt cgcgcgaaat cgtcatcaag gacggtcaga tcctgagcga tcgaacgacc
     3481 aatgagcggt cgcaagtagt gggggcgtaa atgccggcat ttgtatggct gacgattgtg
     3541 acggcactgc gaattctcgg gcgaaacagg ctgcgtgccg gcctgacaat gctgggcatc
     3601 gtcatcgttg tgggagcggt catcgcgatg gtaagcatcg gcgagggggc aaagcaggcc
     3661 gtccaaacgc agatcgccac catgggcacg aatgtcatca tgatctggcc ttcctcgacg
     3721 agtgtcggtg gtgtgcgggg cgcacaaggc ggggcggtca cactgactgt tgcggatgca
     3781 gtggatctga agaagaaaat tccggttcta tctgatacgg cttggatgaa acgtgatgtg
     3841 ttgcaggtcg tgaacggtca cctgaacggt aacagcccgg tcaatggagt gtcacccagc
     3901 tatctcagta tccgagactg gacgttcagt aagggaggtc cctttaccca ggcagatatg
     3961 gatagcgtcg ctctcgtcgc acttcttgga cagtcggtgg tcgatgaaat ctttgatcca
     4021 ggagaggaac ccctcggggc catcattcgt attagaaacg tcccgctccg cgtgatcggc
     4081 gtcttatccc ccaaaggaca gagcacgtat ggtcaggatc aggacgatgt cgtctttatc
     4141 cccttcacga ccgccgaacg aaaagttttt ggaacgctgt tccttggatc ggtcggcggc
     4201 ctatatgccg ccacagatcg agccgaggat atgccggaag cggtagaaca aatacgagag
     4261 gtcatgaggt ctcgccaccg tctacaggcc gaccagccgg acgattttac gattcgcacg
     4321 caggtcgaat taggaaaggt acaggagggt acaagtcaaa ccttgacggt catgttgatg
     4381 gcgattgcat ccgtctccct actcgtcggc ggtatcggca tcatgaatat tctgctcgtc
     4441 tcggtgacgg aacggacaag ggaaatcgga gtccgtatgg cggttggagc caagcgagcc
     4501 catatcctga ttcagttctt gatcgaagcc atgacgctca gtgtggtcgg cggttgcatc
     4561 ggcattctat tgggagtgct gagcgcccgt ttgacgaccg tgattgccgg ctggcccacg
     4621 attatttcgg gtgacacggt cgcgacggca ttcggctttt ccatcgccgt cggcttgttt
     4681 tttggtttat atcccgccaa taaagccgct cgactcaatc ctatcgacgc gctgcgctac
     4741 gaatagagtt ggtacgcact tctcaaggat ccatcgcgat gattgtttca atagcccgtg
     4801 tccgagtctc gtcggtctgg tgcgcgaggt cgacgaccaa tggggtgacg tactcggtgc
     4861 ctcctggttg gtgagtaatg gtgacgatcg gtagatgcag tctttcttga ttgagttccg
     4921 tgaccaccgc caactcttgt gtgttcagtc gcacatgact gtgaaccgga tagataccga
     4981 ctctcgcgat aaatgacgat acgattcgtt gatcgagctt gccgtgccgc gcttcttgat
     5041 acaagcattg aaatgtttga tggggagtga gcggagaagt cccgccgaat ccggtgatca
     5101 attcatcgta tcgatcc
//
LOCUS       DATNDD010000450         3894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_57304, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000450 DATNDD010000000
VERSION     DATNDD010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57304"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VN647_10890"
     CDS             complement(<1..264)
                     /locus_tag="VN647_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97580.1"
                     /translation="MKERQAKSLRNPSSEDATQPILTHEKSLSQKIGQGSEAGDILDQ
                     QLVMLGFQLITQLNTLIKTSKIHGRANAALDKPVETMLTLIETL"
     gene            complement(275..2071)
                     /locus_tag="VN647_10895"
     CDS             complement(275..2071)
                     /locus_tag="VN647_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWG97581.1"
                     /translation="MTTDLKTAQEMMAAAVAARAAEDPEIVSVKRVLKLLDKTAKSNR
                     TYGPTNPVAQKFTQQLFEELTHHLAAHSKLAFLVQRSELLCKDSVVYQAEKDGGSESL
                     AFKLYADGIRELVLHQGLTQEDLSFFLASLWSDVDSNEDDDDIVTRLWSRNLSTITLV
                     TAEELSKVSAGNEGFIRLGSMSSSDSTLRELLDRERERKKRGEAGTDPERGGSGNAKS
                     RFQSGLAGYEVTEEELAVLAQEIEAERKQDGLIYILDMLTAILASEKSSVLLTKLFSL
                     WGTVVESLLREGKWTVLENVLSLLHETDAVRPDLGEEHKQQLASLLNGLGRAERVKVI
                     EAYLNRNLDADVKGLSTILVLMKTDAVSSLCSLLANVTSPVHQAIVSEALAVLAKEHP
                     DFVLRGLSDRRPGYVRNLLSILIKWNNPKFADPVEKLIRYPDAQVRREVVRALGLFRP
                     NGNGMKLVSLTTDEDEGVRFAALKLLMSGQYIAPFSLWAPLLSEEEFMDRPISERRAV
                     FQAVRATCGDEAVPYWQGLMTEWVWMNRRKKEELAVIGAETLGKLATPAAVAALSLGA
                     QKGSAAVRQACVAALSQAQRLQRGVPSTGATP"
     gene            2205..2843
                     /locus_tag="VN647_10900"
     CDS             2205..2843
                     /locus_tag="VN647_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HWG97582.1"
                     /translation="MSSTPLIICFGDSLTAGFQAPTRDNPTGRETPYGQFLQSYLGDV
                     VEVRTSGICGELTGEMVLRFRRDALNHLPGYVPILGGTNDLGWNASPSEIMRNLVTMY
                     EQTLAMGSVPIPVTVPSIRVEDGPGGTAGQEWISEHLDRRSRLNGLIQEYAGSKGLAC
                     TDLFAATVDPKSGQLAERYSNDGIHLTTAGYRLFAEHVTHTLKPLLVQNAQS"
     gene            2840..3163
                     /locus_tag="VN647_10905"
     CDS             2840..3163
                     /locus_tag="VN647_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWG97583.1"
                     /translation="MSSILQAVTDREFDEVVEASAVPVLVEFWKPGCGHCRALMAELE
                     RLQVDRGLSLLILTMNVDENFQIPAELEVSSLPALALYRYGEFVKFIGGLGKQNEIVK
                     QIESE"
     gene            complement(3257..>3894)
                     /locus_tag="VN647_10910"
     CDS             complement(3257..>3894)
                     /locus_tag="VN647_10910"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HWG97584.1"
                     /translation="DTRWQAPNEAGERYLNLIEIPIAEIGTDPGQAKILGERPITLPS
                     YLGVPRTPKDNGTLVEGTFGNVLAAVVDKATTAWYDEHHIAPADRDQMNGYRPNLLQT
                     KKIYKARPLNGIWATAPFLHNGSVPTIYALLSPVEERPMIFCLGDRGYDPVRMGYRTE
                     CSDGTFRLDTSIPGNLNTGHEFRNEPTGNGVIGRALSHQERMDLIEFLKSL"
BASE COUNT          812 a         1068 c         1094 g          920 t
ORIGIN      
        1 gagggtttca atgagggtca acatcgtttc gacgggtttg tcgagagcgg cgttggctcg
       61 gccatggatt ttcgacgtct taatcagagt attcaactgt gtgatgagct gaaatcccag
      121 catcaccaac tgttggtcga ggatatctcc ggcttctgat ccttggccta ttttctgcga
      181 tagcgacttt tcgtgcgtca agatcggttg tgtagcgtct tcgctggatg ggtttcgcag
      241 agactttgcc tggcgttctt tcacaatccg tttcctaggg ggttgcaccg gttgagggga
      301 cgccgcgttg cagccgttga gcttgcgaca atgccgcgac gcatgcctga cgaaccgcag
      361 cgcttccctt ttgggcccca agtgagagag ccgcgacggc ggcgggggtg gcgagcttcc
      421 cgagcgtttc tgctccgatg accgccaact cttctttttt ccttcgattc atccagaccc
      481 attcagtcat gaggccttgc caatacggaa ctgcttcgtc accgcacgtg gcgcggacag
      541 cctgaaagac cgctcgacgt tcactgatgg ggcgatccat gaactcttct tccgacagaa
      601 gaggagccca aagggagaag ggagcgatgt actggcctga catgagcagc ttgagtgcgg
      661 cgaaacggac cccttcgtct tcatcagtcg tgagggagac gagtttcatg ccattgccgt
      721 tcggccggaa cagaccgaga gcacgaacca cttcccgacg cacctgcgcg tccggatacc
      781 ggatcaattt ttcgaccgga tcggcgaact tggggttatt ccacttgatg aggatcgaaa
      841 gcagattacg aacataccca ggcctgcgat ccgataatcc tcgcaatacg aaatccgggt
      901 gctctttcgc caagaccgcg agggcttccg acacaatggc ttggtggacc ggtgatgtca
      961 cgttcgcaag aagcgagcag agcgaggaga cggcatccgt tttcatcagc accaaaatgg
     1021 tggacaaccc tttcacgtcg gcgtcgaggt ttcggttgag ataggcctcg atcaccttca
     1081 cccgttccgc gcgaccgagt ccattgagga gagaggccag ctgttgcttg tgttcctcac
     1141 ccagatcagg ccgcacggca tccgtttcgt ggaggagact taagacgttc tccaacacgg
     1201 tccatttgcc ttcgcgaaga agcgattcca cgacggtgcc ccacaggctg aagagtttcg
     1261 tcagcaacac cgaagacttc tccgacgcga ggatcgcggt cagcatgtcc aggatgtaga
     1321 tgagaccatc ctgttttcgc tctgcttcga tttcttgcgc caggacggca agctcttctt
     1381 ccgtgacctc ataaccagcg agtccggact ggaaacgact tttggcgttt ccggaaccgc
     1441 ctctctcggg gtcggttccc gcctcgcctc gcttttttcg ttcccgctcg cggtccaaca
     1501 attctctgag cgtcgaatcc gacgaactca tgctgccgag acggataaag ccctcgttcc
     1561 ctgcagaaac ttttgagagt tcctcagccg tcacgagcgt gatggttgac aggttccgag
     1621 accacaggcg tgtgacgata tcatcgtcgt cctcattcga gtcgacgtcg ctccataagg
     1681 aagcgaggaa gaacgacaga tcttcttgtg tgagaccttg atgtaggacg agttctcgga
     1741 tgccgtcggc gtacagcttg aaggctaaac tttcgcttcc tccatccttt tccgcctggt
     1801 acacgacaga gtccttgcac agcaattcgg atcgctgaac gaggaaagcc aattttgaat
     1861 gagccgcgag gtggtgggtc aattcttcaa acagctgctg cgtaaatttc tgggcaacgg
     1921 gattcgttgg gccgtatgtc cggttcgatt tggccgtctt atccagtagt tttaagacac
     1981 gtttgacgga aacaatctcc gggtcttcag cagcgcgagc ggcgactgcg gcagccatca
     2041 tttcttgagc cgttttgagg tccgtcgtca tacgtaagac ctaccggcag atctccgctt
     2101 cgtcaagaaa atcgctgttc tttacagccg ttcgactccg aatacgtgct taatccgtgg
     2161 cttgtttgtc acgacaggca tcggtagaat cgagccgcag tactatgagc agcactcctc
     2221 tcatcatctg tttcggcgac agcctgacgg cggggtttca ggcgccgacc agagacaatc
     2281 cgaccggacg agagactcca tacgggcagt ttctccaatc ctatttgggt gatgttgtcg
     2341 aggttcgcac cagtgggatc tgcggtgaat tgacgggtga aatggttttg cggtttcggc
     2401 gtgatgcact caaccatctg ccgggctatg tgccgatcct cggcggaacc aacgatctgg
     2461 ggtggaacgc gtcaccgtcc gaaatcatgc gcaatctggt cacgatgtac gagcagacgc
     2521 tcgcaatggg aagcgtaccc attccggtga cggttccttc gatacgagtc gaggacggtc
     2581 cgggcggcac ggcaggccaa gagtggatct ccgagcatct tgaccgtcga agccgattga
     2641 acggacttat ccaagagtac gccggctcca aaggtcttgc ctgcactgat cttttcgcgg
     2701 ccaccgtgga tcctaaaagc gggcaattgg cagaacggta ctcgaacgac gggattcacc
     2761 tgaccaccgc tgggtatcgg ctatttgccg agcatgtcac gcacaccctg aagccgctgc
     2821 tggtgcaaaa cgcgcaatca tgagttccat tctgcaagcc gtcactgatc gagagtttga
     2881 cgaggtggtc gaggcaagcg ccgtcccggt gttggtggag ttttggaaac caggctgcgg
     2941 tcattgccgg gccctcatgg cggagctcga acgcttgcaa gtcgataggg gattgagtct
     3001 gctcattctc acaatgaatg tggatgaaaa ttttcaaatt ccagctgaat tggaagtctc
     3061 gtcgcttccc gcgctggcgt tgtatcgata cggtgaattt gtaaaattta tcggtgggct
     3121 tggaaaacag aatgagatcg tgaagcagat tgagagcgag taaaatcctc cgacgactcg
     3181 ggctcaaccc ttccgagcct gttgccggtc aaacacaatc cgcactgttg ccggtaatcc
     3241 ccctccctca atcctcctac aggcttttga gaaactctat caggtccatt cgctcctggt
     3301 gactgagtgc tcgtccaatg acaccgtttc cggttggctc gttcctgaat tcgtgaccag
     3361 tattgaggtt gccggggata gacgtatcga gccggaacgt gccgtccgaa cattcagtgc
     3421 gataccccat tcttaccgga tcatatccgc gatcgcccaa gcagaagatc atcgggcgtt
     3481 cctcaaccgg cgagagtagg gcatatatag tcggcaccga tccattatgg agaaatgggg
     3541 cggtggccca aatcccattg aggggcctgg ccttatagat tttcttggtc tggaggagat
     3601 tcggccggta cccgttcatt tggtcacgat cggcgggcgc aatgtggtgc tcgtcgtacc
     3661 aagccgtggt tgccttatcg acaaccgctg cgagcacgtt cccaaacgtc ccctcgacga
     3721 gtgtcccatt gtccttgggc gttctaggaa caccgagata tgagggaagt gtgatcggcc
     3781 gttcgcccaa aatcttcgct tgacccgggt ccgtcccgat ctccgcaatt gggatttcga
     3841 tcagattaag atagcgctca ccggcttcat ttggggcctg ccagcgtgta tcgg
//
LOCUS       DATNDD010000451         2471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2792499, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000451 DATNDD010000000
VERSION     DATNDD010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792499"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VN647_10915"
     CDS             <1..1365
                     /locus_tag="VN647_10915"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWG97585.1"
                     /translation="PYSLAEADLGYEFNDVLEQLCGRGAMVDPSTGSFPKINNSGFVS
                     NYVQKQPTTSSIPVMQAFGKGRLPILESLAREFAVCDAWYSSLPGPTWPNRFFFHAAS
                     SSGMIESPASWSILKSDLFLGYTFQNGTVFDLLDAARIPWCIYEGDEFPQSFAIRGMT
                     KQWIFHDAFRDFSRFAAEVSKPDFKPQYIFIEPNYGRDILPPQDFIGGNSQHPLDNVL
                     SGEQLIRMVYETIRNSPHWPDSLFLITYDEHGGFYDHDIPPATAVPPGDVPNVAQQPT
                     FDFRQYGIRVPSVIISPFTARNLIDSTPYDHTSFLRTLELLFDLPSLTNRDRQAHSFL
                     HLLRSASSPRTVSPIPPAPTELSAPIEMVGQPIETIPEYPSIANFDGRGPVSLQPHHY
                     DWLHVAFLREQAIQQYEQPGELSLDQSRRDLLEIRTISDARRYMSKVRERVKGFKQRL
                     HLLR"
     gene            1902..>2471
                     /gene="recA"
                     /locus_tag="VN647_10920"
     CDS             1902..>2471
                     /gene="recA"
                     /locus_tag="VN647_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWG97586.1"
                     /translation="MSEKDDKKRALDLALSQIEKQYGKGAIMKLGAEERVDVPAISTG
                     SLGLDIALGVGGLPRGRVIEIFGPEASGKTTMTLHCIAEVQKAGGVAAFIDAEHALDL
                     TYAKKLGVQADDLLVSQPDTGEQALEIAETLVRSGAIDLIVVDSVAALTPRAEIEGEM
                     GDAHMGLQARLMSQALRKLTAAIAKSLTTV"
BASE COUNT          580 a          642 c          631 g          618 t
ORIGIN      
        1 ccatattccc tcgccgaagc cgatctcggt tatgaattta acgatgttct cgagcagctt
       61 tgtggtaggg gggcaatggt ggatccgagc accggaagct ttccaaagat taataactcg
      121 ggattcgttt ccaattacgt acagaaacag cccacaacgt cgtccatccc ggtgatgcag
      181 gctttcggta agggacggct accaatctta gaaagcctgg cgagggagtt cgcggtctgc
      241 gatgcatggt actcttctct tccgggtccg acatggccga atcgcttctt ctttcacgct
      301 gcttcctctt ccgggatgat tgagagtccc gccagttggt cgatccttaa aagcgatctt
      361 tttcttggct atacattcca aaacggaaca gttttcgatt tgctcgatgc cgcgcgaatc
      421 ccttggtgca tttatgaagg tgatgaattc cctcaatcct tcgctattcg gggaatgacg
      481 aaacaatgga tatttcacga tgcatttaga gacttttcca gattcgcggc agaagtgtca
      541 aaaccggact ttaagcctca atatattttt atagagccta actacggccg agacatactt
      601 ccaccgcaag acttcatcgg cggaaattca caacatccat tggataatgt attgagtggt
      661 gaacaattaa ttcgaatggt ctatgagacg attcgtaact cgccgcactg gccggacagc
      721 ctgtttctca ttacctatga cgaacatgga ggattctacg accacgatat cccgcccgcc
      781 acagcagttc ctccgggaga tgtacctaat gttgcgcaac agcctacttt cgactttagg
      841 caatatggta ttcgcgtgcc gagcgtcatc atttcacctt tcaccgcaag gaatctcatt
      901 gacagcactc cttacgatca cacctcgttt ctgagaacgc ttgagttgtt atttgatcta
      961 ccgtccctga cgaaccgaga cagacaggct cacagtttct tgcatttatt gcgatcagca
     1021 tcatcccctc gcactgtttc gcctatccct cccgcgccta cagaattgtc agctcctatc
     1081 gaaatggtag gacagcccat cgagaccatc ccggaatacc cctccatagc aaactttgac
     1141 ggtcgcggac cagtgtccct ccagccacat cactacgatt ggcttcacgt tgcctttctt
     1201 cgtgagcaag ccattcaaca atatgaacag cctggagaat tgtcgcttga tcaatcccgt
     1261 cgagacctgc tggaaatcag aactattagt gatgcacgac gatacatgtc taaagtaaga
     1321 gaaagggtga agggcttcaa acagcgacta cacttgctta gataggatgt tatgcggaac
     1381 cccctttgtt cgcctcacca ttgaaccatc catgacttat ctgttcaaca attgaatttt
     1441 gatcctagga ataaatgagg aacggcgtgc tgctaatacc gtgtcactaa tgcataggtg
     1501 tgccgaatga cggatcacgg atcgatcgaa agtcgagaga ttcgaatcga cctcacgatt
     1561 gattttcgat gaaggttgct gagagagtgt caagccttgc cttgggcatt ctgttgatcg
     1621 ggcataagcc tcatggtcca cacaccctgt ctcgattttc ccggtccgtc cctgccacct
     1681 cactgcgcac agcaacggtg tgggtgctcc gctgagccat gctaatcgcc agcattgaac
     1741 gatcgaggtc attccctgag cgtggtgttg ggaaggacga ggagtcttac ttgcctgcat
     1801 cgcctttgaa tgcttccatc ctcagattca gactaggttt cctctggttg cttgggtata
     1861 atccatcgga ttcattcctt tcatcttgtt aggagatctg tatgtccgaa aaagacgata
     1921 agaagcgcgc gctcgactta gccctgtccc agattgagaa gcagtatggg aagggcgcga
     1981 tcatgaagct cggggccgaa gagagggtcg atgtgccggc catttcgacc gggtcgctgg
     2041 gcctcgacat tgctctcggt gtaggaggac ttccgcgcgg acgagtaatc gagatctttg
     2101 gcccggaggc atcaggaaag acgacgatga cgctgcactg cattgccgag gtgcagaaag
     2161 cgggcggggt cgcggcgttc atcgacgcag aacatgcact ggacttgacc tatgccaaaa
     2221 agctcggtgt gcaggccgat gaccttcttg tgtctcagcc ggacacgggt gagcaggcgt
     2281 tggagattgc cgaaacatta gtgcggagcg gcgccatcga tttgatcgtc gtcgattcgg
     2341 ttgcggcgtt gacgcctcgc gcggaaatcg aaggcgagat gggcgatgcc catatgggtc
     2401 ttcaggcgcg gctcatgtcg caggcgctga gaaagctgac agcggccatt gcaaaatctc
     2461 tgacgaccgt g
//
LOCUS       DATNDD010000452         8915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_366772, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000452 DATNDD010000000
VERSION     DATNDD010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366772"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VN647_10925"
     CDS             <1..285
                     /locus_tag="VN647_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97587.1"
                     /translation="WERSRDYLESTKKRLTWLVPFYPPSDGERLMRTRLGMGTLDKMN
                     AQAHWRDEQGLGESFLSPPPMMDTAQMLCRRDGHAGNLTSNYFRDWLTLC"
     gene            complement(824..955)
                     /locus_tag="VN647_10930"
     CDS             complement(824..955)
                     /locus_tag="VN647_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97588.1"
                     /translation="MTRSPADAIGNVALIGAATLQGGSLNGEDFAVIFTGTITPPPA"
     gene            1567..2088
                     /locus_tag="VN647_10935"
     CDS             1567..2088
                     /locus_tag="VN647_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97589.1"
                     /translation="MRSKGLLRILSWMCLIAALALCADVFSRPDAHAKEFWFEAQELD
                     KEKGVFEAGETKIWLPPAVVIDQKEDLNEPLWFIVQNSTGLDHEFAVGGLFMLVPEEE
                     AIPAIKLDPESAGLLPRNIRVPLHIKVKSGQTRRVQVAPRGLVGEENLGARYQFFCPK
                     HKDIRVSRFIYVD"
     gene            2624..3394
                     /locus_tag="VN647_10940"
     CDS             2624..3394
                     /locus_tag="VN647_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWG97590.1"
                     /translation="MRTSKVNAWYGWVPDRPDQRDKLYAAMAAPPKTLPPKVDLRDDC
                     SPIEDQGQLGSCTANALVGNLEFLEKKAGHNPANLSRLFIYYNERAMEGTISEDAGAM
                     IRDGVKSLVKWGVCTEKRWPYKINKFAKKPSPSCYKQAGDYQVTSYHRIIGLQQMKQC
                     LAEGYPFVFGFSVYEAFESPHVAKTGQLNVPKPGEKQLGGHAVMAVGYDESVRRMLVR
                     NSWGTDWGIQGYFTMPYDYISNDNLADDFWTLRAFEGE"
     gene            complement(3919..6267)
                     /locus_tag="VN647_10945"
     CDS             complement(3919..6267)
                     /locus_tag="VN647_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWG97591.1"
                     /translation="MMKTRFISLPVVLSALIFLLDACATNPYLDNETRSTGRLTNGTV
                     LLHASAGPLSVGSGRVITGNDAAFHSKLELVQSATDSIEAMYYIYSDDLSSSVLTQAL
                     IDAAQRGVRVRLLVDYETNYNQLDLFSMMEKYGNIGKGSLEVRLFNRPTRAIVQDAVY
                     VTLGCGAALPNVDKTCGAMKNQEIDRMFQREQVEGRPAAELGISNLNIGNSGLFLSGL
                     YSKKSDLMALAVLAGQSVDVSSLKQPGEAPSVQQRAQLKRVAELYLHTRVGNAFTRLT
                     SKIELMFLFDLFGATLNPLHDALTGYLPIERPQSKESARDWEYFTDYLHHKLLFVDQR
                     HVQLGGRNVEDSYHMRPNQLTTKYVFMDTDLRLDLSVPDSALQHAFEAQWNYVSMVAT
                     VAEVRQHAPNDYSTNHPLVKHAAAACQDKNGTDQLTCIQEKLADHLLTLDQRIEQRHR
                     TMHQHASQYWHDYQFARVVDESPSFPVDKDAFAAYIENLPFYGKPGDPPVTRSYGSVN
                     GQEAEYGKRIHSLWLLGLQNVCTIATAEKPQQVILHSAYFAPPSNLLRMLGQMTNGDL
                     DCRHVRITVLTNSMDTTDLGVVNLLAQQSLKAFQEYYAGQRHKVMSPQIEYYEYQAPS
                     ADESCVRQKGTSVRHESLHTKVSLFGDDIVVGSANGDIRSYMMDSNNAMFIRNAPHLA
                     LQYRTYIDNLLKDDSKLRNRTGYFASKSHDEMIQEDVKEMECSIQKYNLRKHLKPEEI
                     KAAQDRIIELLNEAYRLSQAILAGGRKGSVAEARYNRIFKPI"
     gene            complement(6369..7784)
                     /locus_tag="VN647_10950"
     CDS             complement(6369..7784)
                     /locus_tag="VN647_10950"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWG97592.1"
                     /translation="MRWTGSVLFGYSVAAILATGCATPFERQLGTTAEYHAILKTPVI
                     APSSEKLLQDGPDYLIGIEKGDTDVVQKLKPCAPDSPQGSLGYCLNLDAYPQDSPWRT
                     RIARDNDHSKTLFVSHIARLDNIDHQKIGSKRVCFLYNIYPHSDFPEATDCGRPFAAQ
                     TSRLPLGVMSGLTALEELEQELDERLAKEPPATRPTHVLVMSTGWNTLQAESLDNYQH
                     WTSHLKRAARDEARPFRPLVIGISWPSEWPLISSWLGPVISVFNKEHDADEVGYITVN
                     VLVHKVLNNLKRKHNVQIVVIGHSFGGRVMATALNSRALLFREGASRPTATLMLGLQP
                     AFSIQRFGDGDQGDPYRRFFQASRKYLLTSSTRDWILTFVGITNPVGSSRGLAKAKER
                     PERFEVVSLDKEGQWQREPSSNPCQLVVLDGSEAISGHHDVYGAAVGRLMWEAIRSFA
                     PASSWSAAPQSVQESPCTVGP"
     gene            complement(7825..>8915)
                     /locus_tag="VN647_10955"
     CDS             complement(7825..>8915)
                     /locus_tag="VN647_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97593.1"
                     /translation="PMAPAIPALFWHDCVKEFITLGSPIDKFHTLWYQNYLHMGFPNV
                     SNIPTTWAEGWLQDVKTQITHVNLCDEQDPVGHHLDIAQATSNYSKVFATDTPVAYRD
                     VIFRRYPIPGVAHIEYWRDHRLFRGIIWHVMNLRPTGAPPKPDYFVTDSFRELKGVYG
                     KILVWAYFRISLIAAMATGVLVIYGIGSLRYLGFGIGSVLAVMAACFLWIRPYNTGVY
                     REETDLQLSGNPNLRRVHLFYRRWKLRQGLFANLVAGAVEWRRILILESQGETSSLAD
                     ALKNNTRMSFKTQGGFLSNVWWRYVSGLVWFVAAVACLFWAPKNCHTCHSTAGQLVAQ
                     GLAILSFCYLGTMGFVTWAFFRAKRLKQ"
BASE COUNT         2190 a         2343 c         2383 g         1999 t
ORIGIN      
        1 tgggaacgat ctcgtgacta tcttgaatcc acgaagaaac gcttgacctg gctcgtgcca
       61 ttctatccgc cgagtgacgg tgaacgactg atgcgcacgc gtctcggcat gggcactctt
      121 gataagatga acgcgcaagc acattggcgt gatgagcaag gcctcggcga gtcattctta
      181 tccccaccac cgatgatgga tacagcccaa atgttgtgcc gaagagatgg tcatgccggc
      241 aatctcacgt cgaactattt tcgggactgg ctcactcttt gctgattacc gctcagtcat
      301 cgcgacgatg accgagagct ctctggaggc agctcacgga gcgaactgcc tgttgttgag
      361 ctccgcgtca acggccatga taggaaggcc gcatcaatcc atcgccggcg ccggacctgg
      421 tcagaataaa ccgaataaac cgatcgggaa gtaaaaattc gatgtgtgga catgctcaga
      481 ggcatttttg aagagcacgg cgtcacagtg cagctcaggg acgagcatga aaaaccgtag
      541 gtgaatgggc acggcatcag cctgacaggt ggctttccgc gaattctacc cgctcaaacc
      601 ggcggcttcc ctcgatatcg tttctaaggc ggtttttaag gccagacgac caaacaccga
      661 cttgcaactt gctgcgctca aaggggcagg ctgcaaaaaa acttcgttga tgaaggcaga
      721 agcggtgcaa tctttagctg cctgccctgc aacgttgcct taaataactg gagcgcggcg
      781 acacgatggc agcgtggaaa ctagaccgtt tggtcgaaga atctcacgcc gggggcggcg
      841 ttattgttcc ggtgaagatg accgcgaaat cctcaccgtt caatgagcca ccttgaagcg
      901 tcgcggcgcc gatcagggcg acattgccga tcgcatcggc gggggacctg gtcacgttgc
      961 acgtgcttcg ctcacgagga gcgtggagag cagcactatt actaaccctc tccagagctt
     1021 cattgcatct tcctttcttg taggaaaacc ggatgacaac agactctgga cgctttaggt
     1081 ctctggcctt cgatggtcga atcaattcag tggtgcatcg acaaacactc ccttcggcgc
     1141 ctctctatga ggggccgcag gtcttgaacc tcatctgaga aaataactac cgcgaggcca
     1201 aactttcatg cgcacaaacc gtgcttgaac caagccatga cacttccaat agatagcgca
     1261 ccatgctcga agggagaatg tgacactcct gcaaggagat gccatattga cacttccgaa
     1321 cacagatctt ccgctcagga gatctgagat ggaatggaga acggaaggac actgtgcagc
     1381 gattcatgtg ccggcaaata tcaagatgct tcatgccacc cacagtttgt tcgggatggg
     1441 gctgaagtct gggttaaatg attaatgaga cagctcacca cctctggacg gttcggttct
     1501 tgcgttccct cgaattggtt atcgttactc caacgcatgg cgcatcgacc caaagagacg
     1561 ggtgtcatga gaagcaaggg actattgcga atcctctcct ggatgtgtct gattgctgcg
     1621 ttggcattgt gcgctgatgt gttcagccgg cccgatgctc atgccaagga gttctggttt
     1681 gaagcgcagg aattggataa agagaagggt gttttcgaag cgggagaaac gaaaatctgg
     1741 ctcccacccg ctgtggtcat tgaccagaaa gaagatctca atgaaccctt atggttcata
     1801 gtccagaact ctaccggcct cgatcacgaa ttcgcggtcg gaggactttt catgctggta
     1861 ccggaagaag aggcaatccc cgcgataaag ttggatcctg aatccgcagg gctactcccc
     1921 agaaacatcc gagtaccgct tcacatcaag gtaaagtctg gccagacgag gagagtgcag
     1981 gttgcgccac gcgggttagt tggtgaggaa aacctggggg ctcggtatca gttcttttgt
     2041 cccaagcaca aggatatccg agtcagcagg ttcatctatg tggactagac gtcaggtcag
     2101 cagcacatac cgatcatcat gagacacacg ccgtttaatc acccatcgtc tttgttctaa
     2161 tgcacggacg ggatagccga cggttcggtt cgtcagacca aacgcccttg tacactgcgt
     2221 aatgtccgcc cgactggctt gacagcttaa actttggtga ggaaccggaa ccgatatcgg
     2281 tgctcccgat ttgtgtgcgt gtggtgtggg agcggaacac aggcgtccag agcgggtctc
     2341 aatttatggt ggagtggtcg tacgctacac atcgctcgcc cgaataggaa tggaggtcgt
     2401 ctgcaaatgc gaatacgcga agactgcacg tatagatcca ttcagcacga ctacggatag
     2461 atacaatggt ccttacctag aggatgggat tgcagcgtga acgcgaatct tttagcattc
     2521 ggtgcgactc ccgagggcac atgtatgtag gtaatcacat gcctggtcaa gaagccgcgt
     2581 aggcggtggt atgacaagcg gcgcacaatt gtaggaggat gatatgcgga catccaaagt
     2641 aaatgcctgg tacggatggg ttcccgaccg gcccgatcaa cgggacaagt tgtacgccgc
     2701 catggccgca ccgccgaaga ccctaccgcc gaaggtggat cttcgcgatg actgctcgcc
     2761 tattgaagat cagggacaac tcggcagttg cacggccaac gcgctggtgg gcaatcttga
     2821 gttccttgaa aagaaagccg ggcacaaccc tgcgaacctg agccgactgt tcatttacta
     2881 caatgagcgc gcgatggaag gcaccatcag cgaagacgcc ggcgccatga tccgcgacgg
     2941 cgtcaaatcc ctcgtcaaat ggggggtctg taccgagaaa cgctggccgt acaaaattaa
     3001 caaatttgcg aagaaaccgt cgccgtcctg ttacaaacag gccggcgatt atcaggtcac
     3061 ctcctatcac cggattatcg gcttgcaaca gatgaagcaa tgcctcgcgg aaggctaccc
     3121 gttcgtgttt gggttttctg tctatgaagc cttcgagtct cctcacgtgg ccaagaccgg
     3181 ccagttgaac gtgcccaaac caggggaaaa gcagcttggt ggccatgccg tgatggccgt
     3241 aggatatgat gagtcggtcc ggcgcatgct cgtccgcaac tcctggggca cggactgggg
     3301 catccaggga tatttcacga tgccttatga ctatatctcg aatgacaacc tcgcggacga
     3361 cttctggacg ctccgcgcgt tcgagggcga gtaaatattg ctggcataac gtccaccgat
     3421 tccgcgaaag gaactttcat tccagccact atgctgagca tgaatgaagg cggatggaca
     3481 aggaaatata gatggaaaca cagtaggctc aacacacacg tcgtagaatg agaggttgat
     3541 agggcaatgt tggtcgtcgc gagggtggaa gagcaaacac gtcatctcca tagaagagca
     3601 atgtctcccc acacgaacag tccgatcaag aggaagaatc gcctcctcca gaccccgcaa
     3661 gcttcagaag gccgagcttt gttggagcgc atgaccatgg cgccgatgtc ggcgagagac
     3721 cacgcgtacg acaagaatca gaaaatcaac cgaattttgt tccgactcac cctgcgctat
     3781 ccgacaatta atactgttgt gtcataggcc aactcagcgc cattcaaggc atgatactcc
     3841 cataattccg gccacgcgtg ctcagcgtaa cgctgacccc taccctgctt acttttcaag
     3901 tgtaaatcca ctaacagttt aaatcggctt gaaaattcta ttgtaccttg cctccgcgac
     3961 gcttcctttc cggccacctg caagaatcgc ctgggataag cggtacgctt cattgagtaa
     4021 ttcgataatc cggtcctgtg cagccttaat ttcttccggc ttcaggtgct tccgaaggtt
     4081 atatttctga atcgagcatt ccatttcctt gacgtcttct tgaatcattt cgtcatgaga
     4141 tttggaggca aaatatcccg ttcgattgcg cagttttgaa tcgtctttca atagattgtc
     4201 aatataggtt cgatactgaa gcgccaaatg cggagcattc cggataaaca tcgcattatt
     4261 ggaatccatc atgtagctac gaatatcgcc gttcgcggat cctacaacga tatcgtcgcc
     4321 aaacagggat actttcgtgt gcaaagactc atgtcggacc gaggtgcctt tctggcgcac
     4381 acaggattcg tcggcgctgg gtgcttgata ctcatagtac tcgatctgag ggctcatcac
     4441 tttgtgacgc tggccggcgt aatactcctg aaacgccttg agcgattgct gcgccaacaa
     4501 attgacgacg ccaagatccg tggtgtccat ggagttggtc aagaccgtaa tgcggacgtg
     4561 acgacaatcc agatcaccgt tcgtcatctg tccaagcatg cgcaagagat tcgatggagg
     4621 cgcaaaatac gcactgtgaa gaatcacctg ttgaggtttc tccgccgtcg cgatggtaca
     4681 gacgttctgt aatcccaaca accagaggct atggatccgt ttgccgtact ccgcctcctg
     4741 cccattgacc gagccgtacg atcgcgtgac gggaggatcg ccgggtttgc cgtagaaggg
     4801 taaattttcg atataggcag cgaaggcatc cttatcgacg ggaaacgacg gagactcgtc
     4861 aaccacgcga gcaaactggt agtcatgcca gtactgtgac gcgtgctgat gcatcgtgcg
     4921 gtgccgctgt tcgatgcgct ggtccaatgt cagcaagtgg tcagccagct tctcttgtat
     4981 gcatgtcagt tggtccgtgc catttttatc ttggcaggcg gcggccgcgt gcttgaccaa
     5041 gggatggttg gtcgaatagt catttggcgc atgttgacgc acctcagcca cggtggccac
     5101 catggaaaca tagttccatt gagcctcgaa ggcgtgttgc agtgccgaat cggggacgct
     5161 caaatccaac cgcaggtctg tgtccatgaa gacatacttg gtcgtcagct ggttcggcct
     5221 catgtgataa ctatcctcca cgttccggcc acccaattgg acatgacgct ggtccacaaa
     5281 taacaattta tgatggaggt aatccgtaaa atattcccag tctcgagccg actccttact
     5341 ctgaggtcgt tctatgggga gataccctgt caatgcatcg tgcaggggat tgagcgttgc
     5401 cccaaacaaa tcaaaaagaa acatcagttc aattttgctc gtgagacgcg taaaggcgtt
     5461 ccctacccga gtatggagat aaagctcagc aacccttttg agttgtgcgc gttgctgcac
     5521 ggagggggct tcgccgggtt gtttcaagct gctgacatcg acggactgtc ctgctaagac
     5581 ggccaaggcc ataaggtcgg attttttgga atataatccc gacaaaaata atccggagtt
     5641 gccgatgttg agattggaaa tgccaagctc cgctgctggt cgtccctcga cttgctcacg
     5701 ttggaacata cggtcaatct cctgattctt catcgctcca catgttttgt cgacattagg
     5761 gagcgccgct ccgcaaccta atgtgacata gaccgcatct tggacaatcg cacgagtcgg
     5821 ccgattaaac aatcgaacct caagtgaccc tttccctata ttcccgtact tttccatcat
     5881 tgaaaacaga tccagctggt tgtagttagt ctcatagtcg acaagcaggc ggacacgtac
     5941 tccgcgctgg gcggcatcaa taagagcctg tgtcaggacc gaggaggaca agtcgtcaga
     6001 gtaaatgtag tacatggcct caatggaatc cgttgctgat tggacgagct cgagtttgga
     6061 atgaaatgcc gcatcgtttc ctgtgatgac acggccgctt ccgacgctca gtggtcccgc
     6121 tgacgcgtgc agcaagacgg tgccatttgt cagacgcccc gtcgaccgtg tttcgttgtc
     6181 gagatagggg ttggtggcac aggcatcaag caagaaaatc agcgccgaca gcaccacagg
     6241 taaggaaatg aagcgagttt tcatcatatc ctccaataca cggctagatg agaagacaca
     6301 tcgggtttgg tacgacacag gaacagatgc gcgtgaaaac gtccgacggc gagcgcacgc
     6361 acggacattc atggtccgac agtacagggt gactcttgaa cgctctgggg agcagcagac
     6421 cacgaagagg ctggggcaaa ggagcgaatc gcttcccaca tgagtcgccc cacggctgca
     6481 ccatacacat cgtggtgccc actaattgcc tctgatccat ccaagaccac aagttgacaa
     6541 gggtttgatg aaggttccct ttgccactgt ccctctttat cgagggaaac gacctcaaac
     6601 cgttctgggc gttctttggc cttagccagg ccgcgtgaac ttccaactgg gttggtaatg
     6661 ccgacgaatg tgagaatcca atcacgggtt gaggaagtaa ggagatactt ccgagaggct
     6721 tgaaaaaatc gccgataagg atcgccttgg tcaccgtctc cgaagcgttg aatgctaaac
     6781 gcaggctgaa gccccaacat caaggtagct gtcggccggc ttgccccctc tcgaaacaac
     6841 agcgcacgac tgttgagagc cgtggccata actcgtccgc cgaaactgtg accgatgacc
     6901 acaatttgaa cgttgtgctt acgtttcaaa ttattgagaa ccttatgcac aagcacgttg
     6961 acagtgatat atccgacttc atccgcatca tgttctttgt taaacacgct gatgaccggc
     7021 cccagccagc ttgaaatgag cggccactcg gatggccaac ttattccgat gaccaatggg
     7081 cgaaatgggc gcgcctcatc tcgggcggct cgtttgagat gggacgtcca atgttggtag
     7141 ttgtcgagtg attccgcttg gagcgtattc catccggtcg acatgaccag cacatgcgtc
     7201 ggcctcgtcg ctggcggctc cttcgcaagg cgctcgtcga gctcttgctc caattcctca
     7261 agggccgtca accctgacat cacgcccaag ggaaggcgtg aggtttgcgc cgcaaagggg
     7321 cgaccacagt ctgtggcctc gggaaagtcg ctgtgcggat agatgttata gagaaagcag
     7381 actcgcttcg agccgatctt ctgatggtcg atattatcga gtctcgcgat atgcgaaaca
     7441 aacaatgtct tgctgtggtc gttgtcgcgt gcaatccgcg tccgccatgg gctgtcctgc
     7501 gggtaggcat ccagattcag gcaatagcct agactcccct gcggagaatc cggagcgcat
     7561 ggtttcagct tctggacgac gtcggtatcg cctttctcga tgccaatcag atagtccggt
     7621 ccatcctgaa ggagcttctc cgaagaaggg gcgatcacgg gagtcttcaa gatggcatga
     7681 tactcagccg tggttcctaa ctgacgctca aacggcgtgg cgcatccagt ggcgaggata
     7741 gcagcgacac tgtacccgaa tagaactgat cctgtccatc tcatcgcagc ctcattttta
     7801 tgggtggaac cttccaccat ggcactactg tttcagccgt ttggcacgaa aaaaggccca
     7861 agtgacgaat cccattgtcc ctagataaca gaacgaaagt atggcaagcc cctgggctac
     7921 tagttggccc gcagtgctgt gacaggtatg acaatttttt ggagcccaaa ataaacatgc
     7981 gacggcggcg acaaaccaga cgaggccgga gacatatcgc caccacacgt tcgacaaaaa
     8041 tccgccttgg gtcttaaacg acatgcgtgt gttatttttc agagcatcag ctagcgagga
     8101 ggtctctcct tgagactcaa ggatcagaat gcgccgccat tcgacggctc ccgcaacgag
     8161 attggcgaac aacccttggc gtagcttcca ccgtcgataa aaaagatgaa ctcttcgtag
     8221 gttggggttg ccgctcaact ggagatctgt ctcctcacga tagacgccgg tgttataggg
     8281 acgaatccac agaaaacatg cggccatcac ggcaagcaca gaaccgatgc cgaagcccaa
     8341 atatctcaaa ctacctattc cataaatcac caacactccc gttgccatcg ccgcaatgag
     8401 tgagattcga aagtacgccc acacaagaat cttcccgtac acccccttga gttcgcgaaa
     8461 tgaatccgta acaaaatagt ccggtttggg cggcgctcca gtgggtcgaa gattcatcac
     8521 atgccagatg atgccgcgaa acaaccgatg atcccgccaa tattcaatgt gcgcgactcc
     8581 tggaatggga tacctccgaa agattacatc tcggtacgcc acaggagtgt ctgtcgcaaa
     8641 cactttggaa taattcgagg tggcttgcgc gatgtcgaga tgatgaccaa ccggatcttg
     8701 ctcatcacag aggttcacgt gtgtgatttg cgtcttcacg tcctgaagcc acccttcggc
     8761 ccaggttgtc ggaatattgg agacgttggg aaatcccatg tgcaggtagt tttggtacca
     8821 gagcgtgtga aatttatcga tgggtgaacc aagcgtaata aattccttca cgcaatcatg
     8881 ccagaacaag gctggaatag cgggggccat aggca
//
LOCUS       DATNDD010000453         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2480958, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000453 DATNDD010000000
VERSION     DATNDD010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480958"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..995)
                     /locus_tag="VN647_10960"
     CDS             complement(12..995)
                     /locus_tag="VN647_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97594.1"
                     /translation="MTPPTNQIDEKLDEWGSRLFNVSTETLPRPRSSNRIPLGSAKTV
                     STSGWLSTPQARAAYVRQKLQAMVRRVPQVVVKLVKAPKGMKGISNNLTYISRDGQLE
                     IEDQDGQVIKGKEAVADLKTEWRDGGMPIAADSTMRDAFHLVLSMPTRTDPLAVQRAA
                     RDFATREFSGFQYAMVLHTFETDPDPNPSLHPHVHLTVKAAGLDGVRLNPRKADLQRW
                     REGFAKALREHGIEATTTSRLHRTKQERWTVRHLHEPSKKGEMLEKLKRTTRRQGRTQ
                     EVMRNYEGVMGTLARSDRGEDRQLAADLVHYLSGRSPEVSKTRSPEHDRSS"
     gene            complement(992..1543)
                     /locus_tag="VN647_10965"
     CDS             complement(992..1543)
                     /locus_tag="VN647_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97595.1"
                     /translation="MGRTAKRTTTITVDLGELKAPWQAYCEQQGATPSHALRNAIRRA
                     MDRRATRTPVPRLRVKPKRERASARIELNLTTSELTALKRMAGHEGYVPTKWVVAMVR
                     TKLTGQPHVGQPELETLARSNQQLLALGRNLNQIAKVLNSAPQNRAAFRVEIITELSR
                     VIQAHTKKVSDVLRGTVERWHLQ"
     gene            complement(1922..2122)
                     /locus_tag="VN647_10970"
     CDS             complement(1922..2122)
                     /locus_tag="VN647_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97596.1"
                     /translation="MALAIPVATGDGSEPAIKAAHQAATGNKPLIKQMTRTHRRALVH
                     VRRMVVSSPRERNAAGVHGVTV"
     gene            complement(2886..3260)
                     /locus_tag="VN647_10975"
     CDS             complement(2886..3260)
                     /locus_tag="VN647_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97597.1"
                     /translation="MSAIRITVPGYLPLSVEEIGFSEDGHRLVSLCHYGEQNGDLMRD
                     LDMVFLFHNVPDGAAAEPVSYRNDYLGIVQDVYRYDEAGRRTHVLPMLKQDLKEFARD
                     WFATLRDQGFFAPTAVREILSP"
     gene            complement(3379..>3576)
                     /locus_tag="VN647_10980"
     CDS             complement(3379..>3576)
                     /locus_tag="VN647_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97598.1"
                     /translation="CSSIKASIWKNEGMKGPFYNVTVARSYKGQDGVWKNSESFGLAD
                     LDALVAVSQQAKLWVTERSGR"
BASE COUNT          706 a         1060 c          994 g          816 t
ORIGIN      
        1 ggtccagtac cttaagaaga tcgatcgtgc tctggtgaac gagtctttga gacctctggc
       61 gaccgcccac tcaagtaatg aaccaggtcc gcagccaatt gtcgatcctc cccacgatct
      121 gaccgggcta acgtccccat caccccctca taattccgca tgacttcctg agtccgtccc
      181 tgtcggcgcg tggtgcgttt cagcttctcc agcatctcgc cttttttgct cggttcatgc
      241 agatgtcgca ccgtccatcg ttcctgtttc gtacgatgga ggcgactggt cgtggtagcc
      301 tcaatgccat gctctcgtaa ggctttcgca aacccttccc gccaacgttg caaatcagcc
      361 ttcctcggat tcaagcggac cccatcgagg ccagcagctt tgaccgtcag gtggacatgc
      421 ggatgcagag aagggttcgg gtcgggatcg gtctcgaagg tgtggagcac catcgcatat
      481 tgaaagcctg agaactctcg cgtcgcaaag tcacgggccg cccgttggac tgccagagga
      541 tcggtccgtg tcggcatgga gaggacgaga tgaaaggcat cgcgcatggt ggagtccgca
      601 gcgatcggca tcccgccatc gcgccattca gtcttcagat ccgccaccgc ctctttcccc
      661 ttgatcacct ggccatcttg atcctcaatc tcgagttggc catcacgcga aatgtacgtg
      721 aggttgttag agatgccttt catacccttc ggcgccttga cgagcttcac cacgacttgt
      781 ggcacgcggc ggaccatcgc ttgcagtttt tgccgcacat aggccgctcg agcctgcggc
      841 gtactgagcc atccactggt actcacagtc ttggctgagc caagaggaat gcggttgctg
      901 ctacgcggtc gtggtagggt ctccgttgag acattgaaga gccgactccc ccactcatcg
      961 agcttctcgt cgatctggtt ggttggcggg gtcattggag gtgccatcgc tccacggtcc
     1021 cgcgtaagac atcggacacc ttcttcgtgt gggcctggat cacgcgagaa agttcggtga
     1081 tgatctcgac ccgaaacgct gcccggttct gaggcgcgct attcaggact ttcgcaatct
     1141 ggttcaggtt cctgcccaag gcaagcagtt gttgattcga ccgagccaat gtctcaagtt
     1201 ctggctgtcc gacatgcggt tggccagtca acttggtgcg caccatcgcc accacccatt
     1261 tcgtcggcac atagccttcg tgaccggcca tccgtttgag ggcggtgagc tctgatgtcg
     1321 ttaggttcag ctcgatgcga gcagacgctc gttcccgctt cggcttgacg cgcagccgag
     1381 gcaccggagt ccgcgttgct ctccggtcca tggcccgccg aatggcgttc cgcagggcat
     1441 ggctcggcgt ggcgccctgc tgctcacaat aggcctgcca gggcgctttc agttcgccca
     1501 gatccaccgt gatcgtggtc gtccgtttgg ccgtccgtcc catcagctca cctgcaccat
     1561 tccaagtcaa gacaccccat gccaattaac aacctgtgcc tgattctggt catcggagaa
     1621 gagtccggcg cggtgatcgg agggtcaaag aacatagagc aaaacactac agaaacccaa
     1681 taatgtaaga caactacggg caaaagccat atcctgcaac ctcttaaaat cggtctctct
     1741 ccttaaatac gcacacttgg atcaaggcca gagattaccc tctcacgctt tccaaggacg
     1801 ccaagaacag gcctgaatcg ctggtcgatc gatggagcct ccgtcaagag gggcatgccg
     1861 gcatcgtctc gcacggcgtt gaccgttttc tctcccctaa tagaggggga ttaccctctt
     1921 gttatactgt gacaccgtgc acgcccgccg catttcgctc tcgcggtgag gagacaacca
     1981 tgcgcctgac atggaccaat gctcttcgat gcgttctggt catctgtttg atgaggggct
     2041 tgttgcctgt ggcagcctgg tgagctgctt taatagcggg ttcgctcccg tcgcctgtgg
     2101 cgacgggtat ggcaagagcc acgttgcttc cctgccgcag cccacggcca catcggcgga
     2161 aggtcgctgg acgggcatga cgcccacagg tcgtaccgtc gctggacttg tcttggatga
     2221 cggctcgtat tggtgttcta tacggtaaga ggcaacccca acgtcctggc tggactgatt
     2281 cagggcactg gcacgtcgca ctccggcttc ttcggttcct cgaacatcag agatttcaat
     2341 atggaaggcg ctggcattcg cgcggcgacg atgagtgcca gctatgtgcc gaacaagagc
     2401 tttcacggga cgattgcgta attcaatgga gacacagaga gctttatgag tacgtacgat
     2461 ggagcctcta agtcggcccc gaatatgact caggtggccg ggacctatgc tgagcttcgt
     2521 gccgacaatc acacgatcac ggtgagagtt gattcagccg gtacgctttc aggtcattca
     2581 tccgatggat gcacttttgc tggggacctt gtctcctcgt gcaaagggaa acgtgtttcc
     2641 tcacgctgtg acattcggag gtggcgcgtg tcgccaggta accgagacgg tcatcggggt
     2701 ggctttttta cgatgccgcg atcaaccggc tgtacagtgc cgccctcaac agtgccagga
     2761 ccaccagctt tctctttatc ggcacgaagc ggtaagcgtc actggttcag tgttctgtca
     2821 gggtgtcttt ttttgacggg agcatgatcg ctgaacatca tggcccgtga aatggatcga
     2881 ggagattacg gggacaggat ctctcgaacc gcggttgggg caaagaatcc ctgatctcgg
     2941 agcgtggcaa accagtcccg tgcaaactcc ttcaagtcct gtttcagcat aggcaggaca
     3001 tgggtacgcc tgcccgcctc gtcgtaccga tagacgtctt gaacgatccc caggtaatcg
     3061 ttcctatatg acaccggctc ggctgccgcg ccgtcgggta cgttgtgaaa caggaacacc
     3121 atgtcgagat cgcgcatcaa atcgccgttc tgttccccgt aatggcacag cgacaccaat
     3181 cggtgtccgt cttcactgaa tccaatctcc tcgacagata gcggcagata gccaggcacg
     3241 gtgatgcgga tggcactcac gccgccaagc agctgatcga gatgcttcgc aaaactcctg
     3301 atagatgttc atcgtcttat ttcctttctt cagttgaagg aagcccccgg cgcgagggcc
     3361 agaggcttcc cgtgacgttc agcgtcccga acgctcagtg acccagagtt tcgcctgttg
     3421 ggagacagcc acgagcgcgt caaggtccgc caggccaaag gactcggaat tcttccagac
     3481 tccgtcctgt cccttatacg agcgggccac ggtcacgttg tagaacgggc ctttcatgcc
     3541 ctcgttcttc cagatgctcg ccttgatgct gctgca
//
LOCUS       DATNDD010000454         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2708020, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000454 DATNDD010000000
VERSION     DATNDD010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708020"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1985)
                     /locus_tag="VN647_10985"
     CDS             complement(57..1985)
                     /locus_tag="VN647_10985"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWG97599.1"
                     /translation="MQGRFPQKFLDDLPILPKLLLIPAIPFVSLMLFSMMTYLDVQNF
                     IQGEERLTHLYQLQKTAAQYMRSIADLETAFLGYVISENDRYLERFQEGRRSVMEMDQ
                     ELEIQLPAHHARFEDIRALMKKSFLEKETLLQAIQQGNRTEAIQYVREGRSREIMREV
                     RNWMASFDQEQQGMQQQELSLLSYDRTWTRFLILGGGLVTLCLVILALALIAHSIATP
                     LTALSKVVGSTAGQTIPSIPVFDRKDEIGELTTVMKKMSLQIRKDLDEVQQSEAALKK
                     LNAHLSASEVKYRGIVDHAPLGIFMTKGVRVTFSNRYNQQLAGLDPEAAVDPETFRQR
                     MHPEDSDRVYATFSEAVAAGRPCEIIFRLLQGDGSIRTVLSQRVPIMDLESPDVVYVG
                     FNIDITTLDNLQLQLRRAEKLATLGQVAAGIAHELRNPLVGIGSTAKVLLDDFESGDP
                     KRKEIEVILSETRRLDRIVNQIVDYARPRRPVPIRIDLTRLAGEVSKMLRPRLESKHL
                     AIKVSISPMISEFTADRDLLRQVLLNIVDNAIDATPKGSAPIEITGHELFREERPGLV
                     IQVKDQGVGIPQDLLPNVFQPFVTSGKKHGTGLGLAICQNIIESHEGNIYVTSEVGKG
                     TIVGIWLPLEQKTALERV"
     gene            complement(2063..>2785)
                     /locus_tag="VN647_10990"
     CDS             complement(2063..>2785)
                     /locus_tag="VN647_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWG97600.1"
                     /translation="MRALLVQVREVAQNPNVPILVMGETGAGKEFLARVIHHNSARAK
                     GPFVKISCTALSPMRFERDLFGYERGAFAGAERRKLGLLDQAETGTLFLDEIGDLDLV
                     MQGKLVGIVQDRMFRRLGGVEDISGDFRVIASSYRDLKVEVSGGRFREDLFFRLSAMQ
                     FVVPPLRNRTEDIIPLAKLFMMKYGLELGKEVTDIDQKAVATLQQYLFPGNVRELQNI
                     IERAMMLCIGKTLTSSDLPGAH"
BASE COUNT          627 a          782 c          701 g          675 t
ORIGIN      
        1 atggccgtgc ggataacttg ttcgtcgtcg accacaaaaa cgacggtatt catggatcaa
       61 accctttcca acgcagtttt ttgctccagc ggtaaccaaa tgccgacaat cgttcccttt
      121 ccgacttcgc tcgtcacata gatattccct tcatggcttt cgatgatatt ctgacaaata
      181 gccaagccca atcctgtccc atgttttttc ccggaggtca cgaatggttg aaacacattc
      241 gggagcaggt cctgcgggat acccacacct tgatctttga cttgaatcac cagaccgggc
      301 ctttcctctc ggaataactc atgccctgtg atctcgatgg gagcgctgcc cttcggggtg
      361 gcatctatgg cgttatccac gatattcaag agtacttgtc gaaggaggtc tcgatcggcg
      421 gtgaactcgc taatcatggg tgaaatcgag accttgatcg cgaggtgctt actctccaat
      481 cggggcctca acattttgga cacctcgccg gcgagccggg tgagatcgat tcgtatcggc
      541 accggtcggc gtggccttgc atagtccacg atctgattga cgattcgatc cagtcgtcgc
      601 gtctccgaca agatcacctc gatctcctta cgcttcgggt cgccggattc gaagtcatcc
      661 agcaagacct tggccgtcga tccgatgccc accagcggat tcctgagctc atgagcgatg
      721 ccggccgcca cttgccccaa agtcgccaac ttttccgctc gccttaattg caattgcaga
      781 ttgtcgagag ttgtaatgtc gatattaaag cccacataga cgacatcggg gctctccaaa
      841 tccatgatcg gcacacgttg actcaaaacc gttcggatac tgccatcgcc ttgaagtaag
      901 cgaaaaatga tttcgcaggg tcgccctgcg gctacggctt ctgaaaatgt cgcatacacg
      961 cggtcgctat cttcaggatg catccgttgg cggaatgtct cgggatcgac agccgcttca
     1021 ggatccagcc ccgccagctg ctgattgtaa cgattgctga acgtgactct gactcctttt
     1081 gtcataaaga tgccgagcgg cgcgtgatcc acgatgcctc gatacttcac ctcggaagcg
     1141 gacaagtgcg cattcagctt cttgagggcg gcctctgact gctggacttc atcgagatct
     1201 ttgcggatct gtaagctcat ttttttcatc acggtggtga gttccccgat ttcgtctttc
     1261 cggtcaaaga cagggatgga ggggatcgtc tggccggccg tcgaacccac aacctttgat
     1321 aaggcggtta atggagttgc gatggaatgg gcaatgagcg ccagcgcgag gatgacgagg
     1381 cagagcgtca ccaacccgcc cccaagaatg agaaagcgtg tccacgttcg atcgtagctc
     1441 agcagagata gttcctgctg ctgcatgccc tgttgctcct gatcaaacga tgccatccag
     1501 tttcgaacct ctcgcatgat ctctcgactt cgtccctccc gaacatactg aatggcctca
     1561 gttcgatttc cctgctggat ggcttggagc agcgtttctt tttccaaaaa tgacttcttc
     1621 atcagagcac gaatgtcttc gaatcgtgca tggtgcgcag ggagttggat ttccagctcc
     1681 tggtccattt ccatgacgct tctgcgcccc tcctggaagc gctccaggta tcgatcgttt
     1741 tcggagatga cgtagcccag aaatgccgtt tccaaatcag ctatggatcg catgtattgg
     1801 gctgccgtct tttgaagctg atagaggtgt gtcaaacgtt cttcaccctg tataaaattc
     1861 tggacatcca gataggtcat catgctgaac agcatcaaag agacaaaagg aatggctgga
     1921 atgagaagta gtttgggaag gatcgggaga tcgtctaaga acttttgagg aaaacgtcct
     1981 tgcataaaca ctccgatgaa acctgcgtat tgctaactat gacaccgaca tgcccgcgtc
     2041 aatcgtccag cgccgaaagg actcaatgcg ctccggggag gtcgctgctg gtcaaagtct
     2101 tgcctatgca caacatcatc gcccgttcta taatgttctg aagttctcgc acattgcccg
     2161 ggaataagta ctgttgaagc gttgcgacgg ctttctgatc gatatcggtc acctctttcc
     2221 cgagctccag cccgtacttc atcatgaaga gcttggcgag gggtatgata tcctcggtgc
     2281 ggttcctgag cggcggtacg acgaactgca tcgcgctgag acgaaaaaag agatcctcac
     2341 gaaaacgtcc tcccgacact tctaccttaa gatcccggta ggaagacgcg atcacgcgga
     2401 agtctccgga aatatcttcg actccaccga gacgacgaaa cattcgatct tgcacgattc
     2461 ccaccagctt gccctgcatc acgagatcga gatcgccgat ctcgtcgaga aacagagtac
     2521 cggtttctgc ttgatccagc agaccgagct ttctccgttc cgctccggcg aaggcgcctc
     2581 gctcataccc aaagaggtcg cgttcgaatc gcatcggcga gagggcggtg caactgatct
     2641 tcacaaacgg cccctttgca cgagcgctgt tatggtgaat gacacgggct aagaattcct
     2701 tccctgctcc ggtttccccc atgacaagta tgggcacgtt cggattctgg gccacctccc
     2761 gcacctgaac cagcaacgct ctcat
//
LOCUS       DATNDD010000455         4093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3089494, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000455 DATNDD010000000
VERSION     DATNDD010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089494"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VN647_10995"
     CDS             <1..288
                     /locus_tag="VN647_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HWG97601.1"
                     /translation="PSQGEKTWGGVFEVTDEDVNIMDQFEQDVPQGAYRHLQVTVSAA
                     SGEKELVTTYAANPIGKFKPKEHYLDWVVKGLKQWKFPEEVIQEWESYRPR"
     gene            291..662
                     /locus_tag="VN647_11000"
     CDS             291..662
                     /locus_tag="VN647_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWG97602.1"
                     /translation="MPSLHVPYRFFIGETMPKPKYHILVCTNSRPPGHPKPSCGSAGA
                     AQLLMAFNMGLMQRAVPPGEVLVSATGCLGPCEQGPTVVIYPDNTWYSKVTEADVVTI
                     LDEHVAKGTPAATLNPDSVWK"
     gene            753..1265
                     /locus_tag="VN647_11005"
     CDS             753..1265
                     /locus_tag="VN647_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWG97603.1"
                     /translation="MIIPSHHEHKDHAPTSIGCLVITCSDTRTPETDTSGRLIQKLLE
                     GQGHTVAAYHLVKDEPSQIQFRIAQGTANGEVQVIIINGGTGISRRDSTFEAVSEMLE
                     KRLDGFGEIFRSLTYQEIGSPAIMTRATAGILNGRVLFSIPGSENAVRLAMEKLILPE
                     IGHLVRELTK"
     gene            complement(1324..1758)
                     /locus_tag="VN647_11010"
     CDS             complement(1324..1758)
                     /locus_tag="VN647_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWG97604.1"
                     /translation="MATVSQIANKRPKSIGPKTSIASAARTMRQARVGSLLIKKGKQL
                     VGIVTDTDIVRRAVATGKPLGKLTVEKIMTAPICTIEGSQSVDDAQDMMADLGVRHLG
                     VTKNGDISGIVSVRDLLRHYKRYAQSNISTDENYSEPKIMQD"
     gene            complement(1946..2980)
                     /locus_tag="VN647_11015"
     CDS             complement(1946..2980)
                     /locus_tag="VN647_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWG97605.1"
                     /translation="MKAVLFREHGGPDKLSYEEMPMPKIGPQDVLIRVKACALNHLDI
                     WVRQGSPAYAVPLPHVGGSDVSGTVEQVGTQVEGIAVGTQVFVSPGISCWNCEFCMAG
                     RDNMCRTYNLVGAGMHGGYAEYVKVPFRNVLPMPENVTFEQAAAFPLVSVTASHMLFA
                     LAGLQPGETVLVMGAGSGVGMMAVQLAKLAGARVITTVGSDDKIPKAVILGADAVIDH
                     SKEKVSERVKLLTEGRGVDVVVEHIGPEVWASCVESLSKGGRLITCGATTGGDVKLNL
                     RYLYSRQLTIKGSYMGTRAELVKAAELIGQKRLVAVIDRTFPLREARAAQALMASRKF
                     FGKIVLSAEA"
     gene            complement(3193..>4093)
                     /locus_tag="VN647_11020"
     CDS             complement(3193..>4093)
                     /locus_tag="VN647_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWG97606.1"
                     /translation="QADVFTSQPFGGNPVAVFPDSDGLTDDELQQIAREMNLSETVFV
                     FPPTDPAAVARLRIFTPTQEIPFAGHPVLGTFYVLAQLGRVSTRGQVTHVMQECNIGL
                     FPVELHAEQSRVVRVVMSQPKPEFLDPLDAIDDVYLVGGALGLPKHVIADMKWPLQVV
                     STGLPVLIVPVRTLTAVRSINPDASAIINVCERFGANGIMVFTTVTVESFASVHARMF
                     APKIGILEDPATGSAGGALGAYLVQNGVVEVGPRTDILIEQGYEIDRPSRILVQVESD
                     DDMIQGVKVGGQCVMVVEGELRF"
BASE COUNT          947 a         1250 c         1040 g          856 t
ORIGIN      
        1 ccttcacaag gcgaaaagac ctggggcgga gtgtttgaag taacggacga agacgtgaac
       61 atcatggatc agtttgagca ggacgtgccg cagggagcat accgccacct ccaagtcacc
      121 gtctcggcag cgagcggaga aaaagaactc gtcactacct atgcagcgaa cccgatcggg
      181 aagtttaaac ccaaagagca ttacctggac tgggtggtga aagggctcaa acagtggaag
      241 tttccggagg aagtgattca ggagtgggag tcgtatcggc cgcgataagc gtgccgtcgc
      301 tacatgttcc atatcgattt tttattgggg agactatgcc aaaaccaaaa tatcatattc
      361 ttgtctgtac caattcccgt ccccccggcc acccgaaacc gtcttgcggg tcggccggtg
      421 ctgcgcagct gctcatggcc ttcaacatgg gactgatgca acgcgcggtc cctcccggag
      481 aagtcctcgt gagcgccaca ggttgcctcg gcccttgcga gcagggcccg acggtcgtca
      541 tctatccgga caacacgtgg tattccaagg tcactgaggc ggacgtcgtc accatccttg
      601 atgaacatgt cgcgaaagga acaccggcag cgacgctgaa tccggactcg gtgtggaaat
      661 aaacgtcgtc cccgacgggc ctgcttcgag cggccagatg agactgacgc ttgatcgctt
      721 aaaccgatta ctgatagcgt atcccttgac acatgatcat accaagtcat cacgagcaca
      781 aagaccatgc gcccacctca atcgggtgcc tggtcattac ttgtagcgat acccgtacgc
      841 ctgaaaccga caccagcggc cgactgattc agaaattgct tgaggggcaa ggccataccg
      901 tcgccgccta tcatcttgtg aaggacgaac cgagtcagat ccaatttcga atcgcccagg
      961 gcacggccaa tggggaagtg caggtcatca tcatcaacgg tggcacagga atctcgcggc
     1021 gcgattcgac ctttgaagca gtgtccgaga tgttagagaa gcggcttgat ggattcgggg
     1081 agatcttccg ctctctgacg tatcaagaaa tcggttcacc ggcgatcatg acccgcgcca
     1141 ccgcgggcat cctcaacggt cgcgtgcttt tctccatccc cggctccgaa aacgccgtcc
     1201 gcctcgcgat ggaaaagctc attcttcccg aaatcggcca cctcgtcaga gaacttacaa
     1261 agtgatgtgc tgagcgctga gtttgtgaat ctccacgcaa aaagttcagc acgcagtact
     1321 gcatcaatcc tgcatgatct tcggttcaga ataattctca tcggtcgaaa tattcgactg
     1381 ggcataccgc ttatagtgcc gcagcagatc gcgcacggag acgatgccgg agatgtcgcc
     1441 gttcttggtc acaccgagat ggcgcactcc tagatcggcc atcatatcct gggcatcgtc
     1501 aaccgattga cttccttcga tcgtgcaaat cggagccgtc atgatcttct cgacggtgag
     1561 cttccccagc ggcttccccg tcgccacagc tctccgcacg atatcggtat ccgtcacgat
     1621 tccaaccaat tgcttgccct ttttgatcaa gagcgacccc acgcgcgcct ggcgcatcgt
     1681 ccttgccgca ctggcgatcg aggttttcgg cccgatggac tttggacgct tgttcgcaat
     1741 ctgactcacg gtagccatct cctccccctt tcatattatc gtgtcatatt atcgtggttg
     1801 actgccgacg ttcgaacgcc aaccacgttt ggtccggcgt agattatcat gaatgagcgg
     1861 aacgtgcgag gccgttcgct cgcttggcag gatgtccgtc acaaagtttg gagtggcata
     1921 gtaatacagg cctagtggcc ggacactagg cctcagcaga caacacgatc ttgccgaaaa
     1981 actttcggct ggccatcaat gcctgagcag cgcgggcttc ccggagagga aatgtgcgat
     2041 cgatcacggc aaccaaccgc ttctgtccga tcagttccgc ggctttcacc agttccgccc
     2101 ttgtgcccat ataggatccc ttgatcgtga gctggcggga atagagatag cgaaggttca
     2161 gctttacatc cccaccggtg gtcgcaccgc acgtgatcaa ccggccgcct tttgagagag
     2221 actccacaca gctggcccat acctccggcc cgatatgttc gacaaccaca tcgacacctc
     2281 gtccttccgt cagcaatttg acccgctccg agaccttttc cttggagtga tcgatgaccg
     2341 catcggctcc caagatcact gctttgggaa tcttgtcatc ggaaccgacc gtggtgatca
     2401 cacgcgcacc cgccaacttc gccaactgaa cagccatcat cccaacgccg ctcccagccc
     2461 ccattacaag caccgtttct ccaggttgaa gcccggccag cgcaaacaac atgtgcgagg
     2521 ctgtgacaga aacgagtgga aacgccgcgg cctgttcgaa ggttacattt tccggcatcg
     2581 gcagcacatt tcgaaacgga actttcacat actcggcata gccgccatgc attccggctc
     2641 ccacaagatt gtacgttcgg cacatattgt cccgaccggc catgcagaac tcgcaattcc
     2701 agcaactgat accgggggac acaaagactt gtgttccgac agcgatgcct tcgacttggg
     2761 tccctacttg ctcaacagtc ccggagacat ccgagccccc aacatggggt aagggaacgg
     2821 cataggcagg actcccctgc ctgacccaaa tatcgagatg gttcagcgcg caggccttta
     2881 cccgaatcaa cacatcctgc ggaccgattt tcggcatcgg catctcttcg tacgagagct
     2941 tgtccggccc accatgttcg cgaaacaaca ccgccttcat ttcttcacgt tcccctcaca
     3001 cggttttgaa atgattctta gccggataaa tttcctcatc gttatccaaa ctagaaaggg
     3061 aggtccatta gtctccatct gcgcgcatgc gacgagcacc gccctacctg ataaacattc
     3121 gataatcttg tgtgcgcgtc gcgagagcac aggaggctaa tggatctccc ttcttgacca
     3181 actgcccccc tttcaaaacc tcaactcccc ttccaccacc atcacacact gcccgccgac
     3241 cttcacacct tggatcatgt cgtcgtccga ctcaacttgc accagaatcc gtgacggccg
     3301 atcgatttca tagccctgtt caatcagaat atccgtcctc ggtccgactt cgacgacgcc
     3361 gttctgaacc aggtacgctc caagcgcccc gccggcgctc cccgtcgcag gatcctccaa
     3421 aattccgatt ttcggagcaa acatcctcgc gtggacagaa gcaaacgatt cgaccgttac
     3481 cgtcgtgaag accataatcc cgttggcccc gaatcgttcg cagacattga tgatggccga
     3541 cgcatcaggg ttgatcgacc ggacggccgt caacgttcgc accggcacga tcaagaccgg
     3601 taagccggtc gacacgactt ggaggggcca cttcatgtcg gcaatcacgt gcttcggcaa
     3661 gccgagagcg ccaccgacca agtacacatc gtctatggcg tcgagcggat cgagaaattc
     3721 cggtttgggt tgagacatga cgacccgcac cacacgactc tgttccgcat gcaattcgac
     3781 gggaaataga ccgatattac attcctgcat gacatgcgtc acctgtcccc gagtagaaac
     3841 cctcccgagc tgggccaaca cgtaaaatgt gcccaagacc ggatggccgg caaagggaat
     3901 ttcctgagtc ggcgtaaaga tccgcaaccg agcaaccgca gcaggatcgg tcggtggaaa
     3961 gacgaacact gtttccgata gattcatctc ccgagcaatc tgttggagct cgtcgtctgt
     4021 caggccatcc gagtcgggga aaaccgcgac ggggttgccg ccgaacggct gagaggtaaa
     4081 gacgtcagcc tga
//
LOCUS       DATNDD010000456         6526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3405022, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000456 DATNDD010000000
VERSION     DATNDD010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405022"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VN647_11025"
     CDS             <1..460
                     /locus_tag="VN647_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97607.1"
                     /translation="DSLHHIENVSLFFDEALRALRVGGRIIAIDPYLSWASWPIYRWL
                     HPEPMNCTEDPLILKPQRIDRRPFDANQAVATILFEWNQPRFQSRYPGFSIQHIRRLA
                     CVAYPLSGGFNHPSFLPEWVLTPILRLERTLERIGWLLAFRMLVVLERRS"
     gene            591..1010
                     /gene="ndk"
                     /locus_tag="VN647_11030"
     CDS             591..1010
                     /gene="ndk"
                     /locus_tag="VN647_11030"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWG97608.1"
                     /translation="MSERTLAIIKPDAVKKNAIGDIIHRYERGGLKPIAIKMILMSQS
                     VAEGFYAVHKARPFFGSLCTFMSSGPAVVLVLQGDNAIKKNRELMGATDPAKADVGTI
                     RKAHGTNIEFNAVHGSDSPETAQFEVGYFFPGMEIFR"
     gene            1200..1589
                     /gene="gcvH"
                     /locus_tag="VN647_11035"
     CDS             1200..1589
                     /gene="gcvH"
                     /locus_tag="VN647_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWG97609.1"
                     /translation="MIPSDLRFHKEHEWIRLDGKRATVGISDFAQDALGDIVFLELPK
                     VGVSVKVGQQIGEVESTKTTSTIYTPVSGTVSQINTALKDNPEVVNSDPYGKGWMAVI
                     ELADSGEVDQLMTASQYEAFLASQKQG"
     gene            2052..2309
                     /locus_tag="VN647_11040"
     CDS             2052..2309
                     /locus_tag="VN647_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWG97610.1"
                     /translation="MKSVALTEPSKTAVHSRLDLNRASAGELESLPGIGAVLAQRVIA
                     FRESAGGFRTVEDLREVKGIGAKTFDRLKSLVTVSMAKSEE"
     gene            2327..3553
                     /gene="tyrS"
                     /locus_tag="VN647_11045"
     CDS             2327..3553
                     /gene="tyrS"
                     /locus_tag="VN647_11045"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWG97611.1"
                     /translation="MKAINEQLELIQRGAVEIIQQVELEIKLKRAVSEHRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHHVIFLIGDFTGMIGDPTGVSETRKALTKEQVL
                     DNAKTYQRQIFKILDPAKTLIEFNSRWMKEMSAEGLIQLAAHYRVARIMERDDFHKRH
                     QEQKPISVHEFLYPLVQGYDSVVLKADVELGGTDQKFNLLVGRELQRDYGQESQVVIT
                     MPLLEGTDGVKKMSKSVGNYIALEDKAGEMFGKVMSISDTLMYRYYELLTTEDLGRAK
                     ALHPMEAKQALAQLLVERYHGTEAGQQARAEFQQKFQAREFPDEPDRHVDLKLQDMSE
                     IDVGQGSSIKLARLIVKTGLISSSSEARRLIIQGGIEVDGEKETNPDKLVSFEPHQQR
                     RLKVGKKKFAVAEFRP"
     gene            complement(4617..5546)
                     /locus_tag="VN647_11050"
     CDS             complement(4617..5546)
                     /locus_tag="VN647_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWG97612.1"
                     /translation="MPLPFTPNDAEHWQATYLEHCTLHLSPETVATYRKRLAQFVRWW
                     RTEPSTSIFSRELGYRYHQWLTRNETPLRSQALALSSVRAWGTFLVAQGRLTYNPWLH
                     LQGPGKIASLATNFLTYGQIKRLLNAFDRRKLSEHRDAVLVYLMLKTGVRETELSRAT
                     VGHLKPFGQEGGTLTVHSKGKRRKQEEVVLLVPKAWNVLQAYLAARECRSPLQPDAPI
                     FTTIIDGDEHAMTPHRMRRRIHLAYERAGIPTKFFSALSLRHTAAMQALLKKAPLSAV
                     QALMRHEDIATTKNFAEQLTRLQRSGAHYLDHY"
BASE COUNT         1595 a         1616 c         1791 g         1524 t
ORIGIN      
        1 cgattcgttg catcacattg agaatgtctc cctctttttc gatgaagcac tgagagctct
       61 ccgtgtcgga ggcaggatca tcgccataga tccctatctc tcatgggctt cgtggcccat
      121 ctatcggtgg ttgcatccgg agccgatgaa ttgtacggaa gatcctctca tccttaagcc
      181 tcagcggatc gacagacgac cgtttgacgc caatcaggcc gttgcgacga tcttgttcga
      241 atggaatcaa cctcgctttc agtctcgcta tccgggattc tccatccaac acatccggcg
      301 attagcctgt gtcgcatacc cccttagcgg tggattcaac catccgtctt ttctgccgga
      361 atgggtactg acaccgattc tccgactgga gcgaactctg gaacgaatag gttggcttct
      421 cgcctttcgg atgctcgtgg ttctcgagcg tcgatcgtaa agctgaatcg accacgatgt
      481 cgctcactgc tgtgagaccc tcggatgctt gcaaggtaga agaggccgct cctataatgc
      541 accgcacctt agaatggaag ctttaactat cgggaggaaa cagagcgatc atgagtgagc
      601 gaacgttggc catcatcaaa ccggacgcag tcaagaaaaa cgccattggg gatattattc
      661 atcggtatga gcgaggggga ttgaaaccga tcgccatcaa gatgattctc atgtcccagt
      721 cggtggcgga gggattttat gccgtgcaca aagcgcgccc tttttttggg agtctctgca
      781 ccttcatgtc ttcgggacct gcagtcgtcc tcgtgttgca gggggacaac gccatcaaga
      841 agaatcgcga gttgatgggg gcgaccgacc cggcaaaagc cgatgtgggg actatccgca
      901 aagcgcatgg aacgaatatc gaattcaacg ccgtgcatgg ttcagactcg ccggaaaccg
      961 cgcaattcga ggttggatat ttctttcccg gcatggagat tttccgctga ccgtcctgtg
     1021 ttgaagcgcg aggatctcct cgcgctctcc acctctgacc ggtacgatcg ttccttgaca
     1081 acgccggtcc gccccgacta ccatccggaa gaatctttca ggttcacgtt gaggttgagc
     1141 agtaggttcc ctttccttga cctcaacctc tatcactgat ttctcaaaac ggagccgaca
     1201 tgattccatc cgatttacga tttcacaaag agcatgaatg gattcgccta gatgggaaac
     1261 gagcgactgt gggtatcagc gactttgcgc aggatgcttt aggcgatatc gtttttctgg
     1321 aattgcctaa agtgggcgtt tcggtcaaag tcggtcaaca gatcggcgag gtcgaatcga
     1381 caaaaacgac ctctacgatt tatacgccgg taagcggaac ggtgagccaa atcaatactg
     1441 cccttaaaga taatcctgaa gtggtgaact ccgaccccta cggcaaaggt tggatggccg
     1501 tgatcgagct tgccgattct ggggaagtcg atcaactgat gacggcttcg cagtacgagg
     1561 ccttccttgc cagccagaag caaggttaag gcttaggtcg aggttaagcg ctggaaatct
     1621 cttttatact ttataacctc gcctgcgcgc ctcaaccttg ctatgtccga tcaacccaca
     1681 tcctgattct ctgtgctgga cccggcggat atgtcgcggc tcaagtggct gggatgatcc
     1741 atatgcatcc ggcgcttgct gaaggatatg gaaagatatg atggagacca tggaggatgt
     1801 cggggagctc cgttatacac cttattcgtt cgaaagaagg caagtgcatg attaaatccc
     1861 tatttctgaa actcggtatg ttgtccttga caatcggttt ggttatctgg gtccgatgga
     1921 ctccacaacc gcccgctcaa ggcatcccat cagcgccaga gcaaccgatc gtcgcttccc
     1981 aggctacgga gttagaagag cggaacagca ggactgtcgg ttcatctagt cagaatgtac
     2041 agactccgaa catgaagtcc gttgcactga cggagccatc aaaaacggcg gttcattcgc
     2101 gactcgacct gaatcgggca agtgccggtg agctcgaatc gttgccagga attggagcag
     2161 tactggctca acgtgtgata gcctttcgcg aatcggccgg aggctttcgt acggtcgaag
     2221 atctccgtga agttaaaggg attggggcaa aaacattcga tcgcctcaaa tccttagtga
     2281 cggtctcgat ggcgaaatcc gaagagtgac ggagcaaaca aagacgatga aggcgataaa
     2341 cgagcagctg gagctgatcc agcgcggcgc tgtagagatt attcaacagg tcgagctgga
     2401 gataaaactc aagcgggctg tttctgagca tcgtcccttg cgcgtcaagg cgggattcga
     2461 tccgacggcg cccgaccttc atcttgggca tacggtcttg attcataaat tgaagcactt
     2521 ccaagatctc ggccaccacg tcatcttcct catcggtgat ttcaccggca tgatcggtga
     2581 tccaaccggc gtatccgaga ctcgtaaagc attgaccaag gaacaggtgc tggacaatgc
     2641 caagacctat cagagacaaa tcttcaagat tctcgatcca gcaaagactc tgatcgaatt
     2701 caacagcaga tggatgaagg aaatgtcggc ggaagggctc attcagctgg ccgcgcatta
     2761 tcgagtggct cgcataatgg agcgtgacga cttccataag cgccatcagg agcaaaagcc
     2821 gatcagtgtc catgagttct tgtatccgtt ggtacagggt tacgactccg ttgtgctcaa
     2881 ggcggatgta gaactggggg ggacggatca aaaatttaac ttgctggtag gacgtgaatt
     2941 acaacgggac tacggccagg aatcgcaagt cgtgatcacg atgccgttgc tcgaaggcac
     3001 ggacggcgtc aagaagatga gcaagagcgt cgggaattac atcgcactgg aagacaaggc
     3061 gggggaaatg tttgggaaag tcatgtcgat cagcgacaca ctgatgtatc gatactatga
     3121 attgctcacg acggaagatc tcggtcgcgc caaagcgctc cacccaatgg aagccaaaca
     3181 ggcactcgcc cagttgctcg ttgagcgtta tcacggaacg gaagccgggc agcaggcgag
     3241 agccgagttt caacagaaat ttcaagccag ggaatttccg gacgagcccg acagacatgt
     3301 cgatctgaag ctgcaagata tgtcggagat tgacgttggg cagggtagca gcatcaagct
     3361 tgctagattg attgttaaaa caggcttgat ttctagcagt agtgaagcaa gacgattgat
     3421 cattcagggt ggaattgaag tagacgggga gaaggaaaca aatcccgata agttagtctc
     3481 ctttgaacct catcaacagc gccgtcttaa ggttgggaag aaaaagttcg ccgtcgcgga
     3541 atttagacct taaggggaac gggtcacttc cttgacttgt gatggaacgc cagcgtacga
     3601 tccgttgcag ctagtgggcg tagctcagtg gtagagtcct agcttcccaa gctgacccag
     3661 ggggattgcc tatgctgttg atcgcatagg taattcccct gtcgttatta gcaataatga
     3721 gattgggcct tctgttcctc tctgctttga tctggccctt ccctcactaa attatctctc
     3781 tcgctaacac tgtaataaca cttggcgggg ataccctttg gacgagtggg gggaaagggc
     3841 tgctgcgggt accggtacct catgttacgg ccagatttac ttggaattct gtagatcgcg
     3901 gaggggcatc tagctcctaa aggggagcct agattttttc tgaaaaacaa aaaggagcag
     3961 ggcttggcaa ggggagggcc acttatgagt acacctgtaa cgaggggatg agccacacat
     4021 tactctctcg ttttaataat ctgtccaact tatattattc atcctcaatc gttgacccta
     4081 gtaccggcat cccttgattt ttacattctg tttgagccca acaaggaaaa ggggaaggcc
     4141 agtgcagggg gcggcaccca gctaggagaa tcttctccca ggaaatgagg gggttcctat
     4201 gtgaggttgc ttctctcttt tgagccacta cggaaagaaa gacgcgctaa tacgcaacgg
     4261 cgaacaccta tagctatcgc cggagtttgt ccgactcgcc tgttccaaag tgtttcccaa
     4321 cagtttgata tcggttcccg tcggatgggc gttgtacttt tcaatctcgt cttcctcagg
     4381 cctaaaagaa gtcgtatcgg ctcgtttttc gtcggatgaa gccatgagac gagacgatac
     4441 cacttgtcat gcaccaaacc attcctggaa gccatagggc gcaacactgg cagtgaaatt
     4501 gtagagcaga gtatcagtaa gacggaatag aatcgcgcgt tttcggcgta agaatcactg
     4561 gagagttatc gtttataccc ggagtcagta agctgctata gacgtgttcg gatgtcttag
     4621 taatgatcca agtagtgggc gccggagcgc tgcaggcgag tcagttgctc agcgaagttt
     4681 ttggtcgtgg cgatgtcctc gtggcgcatg agggcctgga cagcactgag tggagccttc
     4741 ttcagaaggg cctgcatggc tgccgtatga cgaagcgaca aggccgaaaa gaacttggtc
     4801 gggattccgg cccgttcgta ggctaggtgt atccgccgac gcattcgatg gggggtcatg
     4861 gcgtgttcat cgccgtctat gatggttgta aagatcggcg catccggctg cagcggagag
     4921 cgacactcgc gtgcagcaag ataggcctgg agcacgttcc aggctttggg cactagcaag
     4981 acgacttcct cctgcttccg ccttttcccc ttactatgaa cggtgagcgt gcccccctct
     5041 tggccgaacg gcttgaggtg gcctaccgtg gctcgcgaga gttccgtctc tcgaacgccg
     5101 gttttgagca tcagatagac aagcacggca tcccggtgct ctgagagctt acggcggtca
     5161 aaggcgttga ggagacgttt gatttgtccg taggtgagaa aattggtcgc gagcgacgca
     5221 atcttgcctg gtccctgtaa gtgaagccag ggattatagg tcaggcgccc ctgcgccacc
     5281 aaaaacgttc cccaggcgcg aacactggac aaggccagag cttgcgatcg taagggagtc
     5341 tcgttgcgcg tgagccactg atgatagcga tagcccagct cgcgtgaaaa gatgctggtc
     5401 gatggttcgg tgcgccacca gcgcacgaat tgcgccagcc ttttccggta cgtggccacc
     5461 gtctcagggg agaggtgaag tgtgcagtgc tccagatacg tagcctgcca gtgttcggcg
     5521 tcattcggtg tgaaaggaag cggcattaca acacggcccc gtatataacc agctatctcg
     5581 gcatactcag ggaaatgata ggtacgtgta tactataaac atacccatcc atcaacaaaa
     5641 aacataggta aacagaattt atgactatta tacttgactc atggtgtatg ggtagaaatg
     5701 gtatacccat acctcaacat caatatggag cgccccgaca gggcttggac ctctaacccc
     5761 aagatccgta gttgtattca atgaagaaga aacacgggga taattgcaaa aggctcagta
     5821 tacgggggac acgaagggac accagcggga catgtgacta caacaattca acccccgcgc
     5881 aaccgatttc tagttgtcag cttcactagc taggccccgg ggagtagcct tgcgctgaag
     5941 gaggctggag gtgactatgg gtcctgaggt acctcgtgtt gcagtcgcct taaagaccag
     6001 ccgagctgcc ggggaggggt ggtgggtgtg tctctgtctg gttcctctgc atcgtctaga
     6061 ttttttccga agtcacaaaa aggaggaagc cgtttagcga gtcagactag ccacctgagt
     6121 ttacaaatca gtgacaaggg gttgctcaag tccttgtaga tctggacact cccctctccc
     6181 tataatggaa cacacagttt tcgttctaat ctgatttcac accatttcac ttcaattcag
     6241 tccgcttata gcatcaggag aaaatggtag ggtggtgtaa ttagtcgcac cttgacgcaa
     6301 tgctcactta atctaaaccg actttctctg tgcggcaacg tcctagaggg ataagaaggt
     6361 ttggcctgtc cgggtagcag cgtgagaaca gaccacgacg ctaggcatga ataatcaaca
     6421 aagagccccg agttgtcatt cccaattggg atgcgcattt gaatgcctca tctggcgcac
     6481 catatcatcc agttgtctgg agcgcatccg gcccgcgccg cgtatt
//
LOCUS       DATNDD010000457         4785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1436272, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000457 DATNDD010000000
VERSION     DATNDD010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436272"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..531)
                     /locus_tag="VN647_11055"
     tRNA            complement(456..531)
                     /locus_tag="VN647_11055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(496..498),aa:Arg,seq:acg)
     gene            complement(723..1049)
                     /locus_tag="VN647_11060"
     CDS             complement(723..1049)
                     /locus_tag="VN647_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWG97613.1"
                     /translation="MTDYHRWFLICLSLLITMACSSTGPTPTGETATLTWDANSEPDL
                     QGYKIYLATTSGGYGASIATLPMDVTSYTVTGLETGTTYFFAVTAYNSSGAESSFSKE
                     VSKTFL"
     gene            complement(1378..1572)
                     /locus_tag="VN647_11065"
     CDS             complement(1378..1572)
                     /locus_tag="VN647_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97614.1"
                     /translation="MKSMLMAVMAVAVAVTFSAPAFAGDQKKEEKKGHVVVYGEDKKK
                     EEKKGGHADTFGDKEKKKGE"
     gene            complement(1971..2135)
                     /locus_tag="VN647_11070"
     CDS             complement(1971..2135)
                     /locus_tag="VN647_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWG97615.1"
                     /translation="MIEIKGPKRAKDLLRELNLLAEAHLVIRGDELVTEDEMLSDKDQ
                     IEIRPVISGG"
     gene            complement(2253..3635)
                     /locus_tag="VN647_11075"
     CDS             complement(2253..3635)
                     /locus_tag="VN647_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97616.1"
                     /translation="MPTRFRLEDFALREVLHRVEHTIRRGGGRVWLVGGSVRDLVLGR
                     QPRDLDLEVVGLPPGQVHALLTEHFSVQFVGKAFEVFKLQGLPVDVSIPSRIVLDDSS
                     LPGLLRQADPGMPIDEALARRDFTINAMAWDPDTMELRDPFNGRGDLDSRILRHASTR
                     FADDPLRVLRGMQLSARFELTVAPETVALCRTLSQDGQPGERLWEEWKKLLLQGRKPS
                     LGLQFLRHCGWLRFYPELAALEGCPQDPIWHPEGDVWIHTLHCLDWFAAERTGHEQDD
                     LAVGLGVLCHDFGKPATTRKEYGHVISRGHESEGEGPTRRFLERLTNQHDLIDEVVPL
                     VYCHLRPRALHDANASDGAVRRLAKQVHRIDRLVRVARADHAGRPPKQFDGFPAGEWL
                     LTRAQRLAVHRRAPLPIVMGRHLLELGIQPGPDMGRLLDDCYEAQLDGDFGTLEDGLA
                     YVKSRLSAPC"
     gene            complement(3636..4640)
                     /gene="galT"
                     /locus_tag="VN647_11080"
     CDS             complement(3636..4640)
                     /gene="galT"
                     /locus_tag="VN647_11080"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWG97617.1"
                     /translation="MPDLRRDPIVGRWVIISTERRGRPHDFVKLQPPRPVSTALCPFC
                     PGQERLTPKEIMAYRPQPSGPNFPNWTVRVVPNKFPALQVEGDMGREGVGLYDRMNGV
                     GAHEVIIETPNHIESLADLPTKKIEDMLWAYRDRMIDLRKDLRFRYILIFKNHGASAG
                     ATLEHSHSQLIALPIVPTNVQEELDGCRNHYQQKERCIYCDILRQDLSDGDRIVAENS
                     EFLCVTPFAPRFPFEMWLLPKRHAAYFEESQKSQFEFLAPILSESLRRMDKVLSRPPY
                     NFILHSSPLHEKTGDYYHWHLEVIPKLTQVAGFEWGTGFYINPVSPEEAATFLREAVI
                     "
BASE COUNT         1097 a         1247 c         1377 g         1064 t
ORIGIN      
        1 atgcagccag ataacattat cactacaaat agtaagccac acgatttgat agtgttcata
       61 atataccccc tagttatctt tcacgtagct attctcttga cgcagtgtaa caaaccatat
      121 tctttgttgc tcctaggaga aacgataggt gaggacagtt cgatgagccc gccaagaatg
      181 tcgttctatc gcatcaacac ggcgatagag acaggcctga gacgagagga gcagtttagg
      241 ttgagatggg gccggcagca agccgacgag taatggccgg agtcgatctt gtcgcagtga
      301 aggaattatc agacaccgtg atattgagac gacgctgaga tatgtccacc ttgcaccagg
      361 acaccttcgg gatgctgtga atcgagggag tctaggtgga accgtgacca ctcaggagag
      421 gggaggagcg gaagagaagc aacccattga ttctatggtg cgcccgacag gacttgaacc
      481 tgtaaccccc agatccgtag tctggtgctc tatccattga gctacgggcg cgtgccgaac
      541 cggtgctgag gtgtgagttc tgagttaaag ggctagcttg aggacgatgg gtcaggaagg
      601 agtttcgtct caggacttag cactttcgtc tcagcacttc ccggagtcag tggttataca
      661 gttctcggag agagagggtc aagagacgaa cactgaaaaa gtccggagtc ggcgaaggtt
      721 agttagagga aagtcttgct gacttccttg gagaaggaac tttcggcgcc actcgaattg
      781 tatgcggtaa ctgcgaagaa gtaggtagtg ccggtttcga gaccggtcac ggtgtagctg
      841 gtgacatcca tcggcaacgt ggcaatcgat gcaccatacc cccctgaggt cgttgccaga
      901 tagattttgt atccctgaag gtccggctcg ctattggcat cccatgtaag agtggccgtt
      961 tcaccggttg gcgtggggcc ggtcgaagag cacgccatgg taatcagcag agacaaacag
     1021 atcagaaacc aacgatgata atcggtcatt gatctcgata agtccatatc acactcttac
     1081 cgcgtgatgg tccattcgga atctatacac gagcaaagtt tgttccggtt tcagcgtggg
     1141 ggacattctc gcagaagtcc gtggaaagat aaagaatgag agggtcgccg gcagtgatca
     1201 cgaaagtgag gcgttgaggc tgtaatcaga atcgtacgat gtggccggaa tttgttactt
     1261 tttatcgctg taagagggac caagttggga aaatgtggtt ctactccagg gaaacaaaaa
     1321 tgccgggggc ttcctactgt gtagaaagcc ccccagcccc agcttcacga agctgaatta
     1381 ctcccccttc ttcttttcct tgtcaccgaa ggtgtcggca tggccgccct tcttctcttc
     1441 cttcttcttg tcttcaccgt acacaactac gtggcccttc ttctcttcct tcttttggtc
     1501 gccggcgaag gctggtgcgc tgaacgtcac tgctactgcc actgccatga ctgccatcaa
     1561 catactcttc attataagac cccctctaaa aaaattataa attatgtgtg ccactcactt
     1621 ggcacttgtc cagatgttca gcaagggtag tgccgttctg ccctattcgt aagatgaaac
     1681 gcagaccatc aagtaatcag tgagttatga aatttctctc atgaagccgc gcatgattct
     1741 ccatacaggg tgctgtagca caatggtatg gatacgacta aactgcctca ttccagcatt
     1801 aaacgcggtt ggttggcaga caactttgcg ggacgcctcc gtgctgctaa tcccattcgg
     1861 agggttcaag cggagagatc aaggatggga gataggtctg gatgctatgc gagatcagtg
     1921 agtctatcgc gattgaggta ttcggttcct atccttacat ctggccggca tcacccaccc
     1981 gaaatcaccg gacgaatttc gatctgatcc ttgtcggaga gcatctcgtc ttcggtcact
     2041 aattcgtcgc cccgaatcac caggtgtgct tcggccagaa ggttcaattc gcgcagcagg
     2101 tctttggccc gcttcggccc cttgatctcg atcaatcgcg atgggtggtt caattgtacg
     2161 tgcatgagag gataatacgt gttccatcgg ccaatgggca agggtgttaa ggcgcaacga
     2221 gtgagcgcgt gcaaggccgg agaagaagct gatcagcagg gagcggagag tcgactcttt
     2281 acataggcga ggccgtcttc aagcgttcca aagtctccat caagttgagc ctcataacag
     2341 tcgtcaagga gccgtcccat gtcaggacct ggctgtattc ccaattccaa taggtgacga
     2401 cccatgacaa tgggaagagg agcacggcgg tgcactgcca gccgctgtgc tctggtcaat
     2461 agccactcgc cggcgggaaa tccatcaaac tgtttcggcg gacggcctgc gtgatcggcc
     2521 cgtgccacac gcaccaatcg gtcgatgcga tggacttgct ttgccaatcg acggactgcg
     2581 ccgtccgaag cgttggcgtc atggagggca cgaggacgaa gatgacaata caccagagga
     2641 accacctcat cgataaggtc gtgctggttg gtcagtcgct caagaaaacg tctcgtcgga
     2701 ccttctcctt ccgattcatg tcctcgcgag atgacgtgcc catattcctt tctggtcgtg
     2761 gccggcttgc cgaagtcgtg acagagtacg cctaagccga cggcaaggtc gtcctgctca
     2821 tgtccggttc gctcagcagc gaaccagtcc aggcagtgca gtgtgtgaat ccagacgtcg
     2881 ccttccgggt gccagatggg atcttgtggg cagccttcaa gggcggcgag ctccggatag
     2941 aaacggagcc agccgcagtg acgtaagaat tgcaaaccca gcgacggttt gcgtccttga
     3001 aggagcagct tcttccattc ttcccataga cgctcgcccg gctgaccatc ctgggatagt
     3061 gttcggcaca gagccactgt ctccggcgct acggtgagct caaaacgagc agacagctgc
     3121 atgccacgta agacgcgcag aggatcgtcg gcgaatcggg tcgatgcgtg gcgcaatatc
     3181 cgtgaatcaa gatcgccgcg gccgttgaag ggatcgcgaa gctccatggt gtccggatcc
     3241 caggccatgg cgtttatggt gaagtctcgc cgcgccagcg cctcgtcgat cggcatgccg
     3301 ggatcggctt gtctcagcaa gccggggagg gatgagtcgt caagcacgat acgggaagga
     3361 atggaaacat cgaccggcag accttgcagc ttgaagacct cgaaagcttt tccgacgaat
     3421 tgcacggaga agtgttcggt cagcagtgcg tggacttgac ccggcgggag cccaacgact
     3481 tcgaggtcca gatccctggg ctgtcggccg agcaccaggt cgcgtacaga gccgccgacg
     3541 agccagactc ttccgccccc acgacggatg gtgtgttcaa cgcggtgaag aacctccctg
     3601 agggcgaagt cttcaagacg aaagcgggtc ggcacttaga tcaccgcttc gcggagaaac
     3661 gtcgcggcct cttccggtga caccggattg atgtagaaac cggtgcccca ttcgaagccg
     3721 gcgacttgcg tcagtttggg gatgacctcc agatgccaat ggtagtaatc gccggtcttc
     3781 tcgtgaaggg gggagctgtg gaggatgaag ttgtacgggg ggcgggacag cactttgtcc
     3841 atgcggcgta acgactccga aaggatgggg gccagaaatt caaactgcga tttctgactt
     3901 tcctcaaaat acgccgcatg tcgcttggga agaagccaca tttcgaacgg aaaccgcggc
     3961 gcgaacggcg tcacacagag gaactccgaa ttctcggcga cgatccgatc gccgtccgac
     4021 agatcctgcc gaaggatgtc gcagtagatg caacgctcct tttgctgata atgattgcgg
     4081 catccgtcaa gctcctcttg cacgttggtc gggacgatcg gcagagcgat gagctgagag
     4141 tgactgtgtt ccagtgtggc gccggcagac gcgccgtggt tcttgaaaat gagaatgtag
     4201 cggaaccgaa gatcctttct gagatcgatc atgcggtctc gataggccca cagcatgtcc
     4261 tcgatcttct tggtcgggag atccgcgaga ctttcgatat ggttgggggt ttcgatgatg
     4321 acttcgtggg cgcccacgcc gttcatgcgg tcgtagagtc cgaccccctc gcgacccatg
     4381 tcaccttcga cttgcagcgc gggaaattta ttcggcacga cacgcacggt ccagttggga
     4441 aaattgggtc cgctgggctg tggtcgatag gccatgattt ctttcggcgt gagccgttcc
     4501 tgtcccgggc aaaacggaca gagggccgtt gaaacaggtc ggggcggttg gagtttgacg
     4561 aagtcatgtg ggcggcccct tcgctcggtg gaaatgatca cccaacggcc gactatgggg
     4621 tcacgtctca aatcgggcat gggctccctt tattcgaagt tgcaagcgct gagtcccgaa
     4681 tgctcagtgt ctcagagggg gaaaaatttc acaactcaaa actcagcact caactctcag
     4741 cacacagcac ttcctcttag actctccaca gtccggcctc aaatt
//
LOCUS       DATNDD010000458         5093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3405330, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000458 DATNDD010000000
VERSION     DATNDD010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405330"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VN647_11085"
     CDS             complement(<1..567)
                     /locus_tag="VN647_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWG97618.1"
                     /translation="MLTVSRPSTTVPRLIRRGVDLNEQGEVYMIETYFSRFLDRSFEW
                     ISSSGLRIILITVGMLLLFSLLQRVVERFRRLYEGRLFGPTELKRADTLTQVIRDLAR
                     VVILGVGGMMVLSEVGVDLKPLLAAAGLGGLAIGFGAQSLVKDVISGFFILLEDSIRV
                     GDVVEIAGVGGLVEEVKLRTIVLRDETGS"
     gene            complement(881..1792)
                     /gene="lipA"
                     /locus_tag="VN647_11090"
     CDS             complement(881..1792)
                     /gene="lipA"
                     /locus_tag="VN647_11090"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWG97619.1"
                     /translation="MSFIPLDHLRSSSTINHQHSSVSRPRLPSWFKVQAKTGPDYLAI
                     KQTMDRLKLHTICEEARCPNRWECWNARTATFLILGDICTRRCHYCSVQTGKPFPVDE
                     EEPGRVAEAVRALGLRHAVITSVNRDELPDGGAWVFAETIRQTRRLNPTCTIEVLIPD
                     FEGDEKALATVCAEEPDILNHNIETVRRLFPSIRPQGKYQRSIELLAKAKQQGMKTKS
                     GLILGIGETLDEAREVMRGLRVVDCDIVTIGQYLQPTRDHLPVARFYDPSEFALLKEE
                     GLAMGFTHVESGPLVRSSYHAEQQVAG"
     gene            complement(1797..2162)
                     /locus_tag="VN647_11095"
     CDS             complement(1797..2162)
                     /locus_tag="VN647_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97620.1"
                     /translation="MNGGDLDKHQLAGLDNRERGFSRPVEFERVGEGYRAILRYETVR
                     VTTERHPSQDDALATLIQTLHIQGYRQLKTQLSFRNGVYLGSQELWVEYPDPPQTEPE
                     PSGFFDTIKNWFKPRSVNG"
     gene            complement(2166..2531)
                     /locus_tag="VN647_11100"
     CDS             complement(2166..2531)
                     /locus_tag="VN647_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97621.1"
                     /translation="MALSSTDQTDIAAALVRLYVFLAQYLDRCFDEAARKSYPDSELQ
                     RHLEETRRQLMEILSVNPVVKRKLADECDRILTLGASCLKAGAVDSKTREMIQAERTI
                     LQNKTIVLSDLVAVYRALA"
     gene            complement(2697..3572)
                     /locus_tag="VN647_11105"
     CDS             complement(2697..3572)
                     /locus_tag="VN647_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWG97622.1"
                     /translation="MADEHGHGAQQPQAKDNLIPGVKQVIAVSSGKGGVGKSTVAANL
                     ACALAVAGAKVGLLDADLYGPNIPMMMGSTTGPEQKDGKILPVESYGVKLISMAFLVP
                     EEAPLVWRGPMVHQYLQAFFRDVLWGDLDYLLIDLPPGTGDVQLSLSQMVPLAGAITV
                     TTPQEVALYDVRKGMGMFQKVNVPLLGIIENMSYFVCGHCGERTEIFSHGGGERAAAK
                     LGVPFLGRVPIDPAIRDGGDTGHPIVVADPASPQAGAFRDIAKKIVEAVGGTGKAGPS
                     IDSLLKKVKQPFSSN"
     gene            3804..>5093
                     /locus_tag="VN647_11110"
     CDS             3804..>5093
                     /locus_tag="VN647_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWG97623.1"
                     /translation="MTPSHSLEPTIRLIGEQLARLSGGLSPSIFDSRWWSHSAINLAM
                     KDASFKTRLFHFIDVLPVIQDDERVVSLAEEYFGRESSELFGLQWGLKALASTSIGAR
                     LTGKSIRKQIEQMAKTFIAGASIEESVPALSGLWKDRRAWSVDLLGEATISEQEADRY
                     RDQCLNALNELSQATKTWPSIPLLQQDHLGPIPRVQLSLKISALSSRLDSIDPDGSYE
                     SVAARLRPLVDLAQSLPAGLIFDMEQAETKDLILHIFKQLFAEPAYRSYPYAGLALQA
                     YHRETERDVQDLVAWVRTRHAPITIRLVKGAYWDSDTVRYRQAGWPSPLFEHKAETDI
                     HYERLISVLFEHSDLIRPAFGTHNLRTLAAIEAHAESRGLTSDAREYQMIFGMAEPFQ
                     QAMVKLGRRVRLYTPVGRLLPGMAYLVRRLLENTSNES"
BASE COUNT         1148 a         1505 c         1311 g         1129 t
ORIGIN      
        1 gcttcccgtc tcatcgcgca gcacaatggt cctcagcttc acttcttcca ccaagccgcc
       61 caccccggca atctccacca cgtcgccgac ccggattgag tcttcgagaa gaatgaagaa
      121 tcccgagatc acatctttga ccagactttg ggcgccgaaa ccgatggcca ggccgcccag
      181 cccggccgcg gccagcaggg gtttcaaatc cactccgacc tccgagagca ccatcattcc
      241 cccgactcca agaatgacga cgcgggcgag atcccggatg acttgagtca gtgtatcggc
      301 ccgttttaat tctgtcggtc cgaagagccg tccttcatag agacggcgaa accgctccac
      361 cacccgctga aggagcgaaa ataagaggag catcccgacc gtaattaaaa taatccgtag
      421 gcctgacgaa gaaatccatt cgaacgaacg gtctagaaat cggctgaaat aggtttcgat
      481 catgtacacc tccccttgct cgttgaggtc cacgccgcgc cgaataaggc gcggcacggt
      541 tgtggacggg cgcgagacgg tgagcaccca gtgtctttga atctcttgtg gacgccggcg
      601 agtcggtgag ccggccgtgt cctgcgtacg gagctcggga ctgacaccct tccccgcatc
      661 tgcgtgtggt gtctatcccc gtccgaaccg ggggctggct ccgtcgcgga cgacggagcc
      721 agcccctaac aagctgaatt catagtctat atgcgaacag ggtccggaga tcaacaatag
      781 atgtgtgagc agaaaaaacg gaagagaaaa ggtgacatct cagagactgg cgctctccta
      841 gacggactga agattcccga tcctgccgaa acattgttta tcaaccggct acctgctgct
      901 ccgcgtgata ggagcttcgg acgagcggac ctgattctac gtgagtgaat cccatggcga
      961 gaccttcttc tttcagcaag gcgaactcgg agggatcata gaaccgggcg acgggcagat
     1021 ggtctctcgt cggctgaaga tattggccga tggtgacgat atcgcaatcg acgactctga
     1081 gaccccgcat cacctcgcgc gcttcgtcga gcgtctcccc tatgcccaag atcaagccgg
     1141 acttcgtctt catcccctgc tgcttggcct ttgccagtag ctcaatcgac cgttgatatt
     1201 ttccttgagg tctaatcgat gggaagagtc gccggacagt ttcgatattg tgatttagaa
     1261 tatccggctc ctcggcacag accgttgcga gcgctttctc gtcaccctca aagtccggta
     1321 tcaggacttc gatcgtgcaa gttggattca gccgtctcgt ctgcctgatc gtttccgcaa
     1381 agacccacgc tccgccatcc ggcaattcat cacgattcac tgaggtgatc accgcatggc
     1441 gcagaccgag ggctcgcacc gcttccgcga cacgacccgg ctcctcctca tccactggaa
     1501 acggcttccc tgtctgcaca gaacaataat gacagcgtct ggtgcagata tcgccgagaa
     1561 tcaggaacgt tgccgtgcgg gcattccaac attcccatcg attcggacag cgagcctctt
     1621 cgcagatggt gtggagtttg agtcgatcca tggtttgctt gatagcgaga taatccggac
     1681 cggtttttgc ctggacctta aaccaggaag gaaggcgggg tcggctgaca gacgagtgtt
     1741 gatggttaat ggttgatgag gaacggagat ggtccaacgg aataaaactc atagcgctat
     1801 ccattcacgg agcgcggctt aaaccaattc tttattgtat caaagaaacc agatggctca
     1861 ggttcggtct gtggtggatc tggatactcc acccagagtt cctgtgatcc cagatagacc
     1921 ccattgcgaa aactcaattg agtcttgagc tgtctgtatc cttgaatgtg caaggtctgg
     1981 atcaaggtgg cgagcgcgtc atcctgggac ggatgtctct ccgtcgtgac gcggactgtc
     2041 tcataccgca agatggcccg atatccttcc ccaacacgtt caaactcgac cggccgacta
     2101 aatcctcgct ccctattgtc caatccggcg agttgatgct tatcgagatc tcctccgttc
     2161 atcgttcatg ctaaggctcg atagaccgca accaagtcgc tcagcacaat ggttttgttc
     2221 tggagtatgg tccgttcagc ttgaatcatc tcgcgagttt tggaatcgac ggcaccggct
     2281 tttaaacaag aggcaccgag cgtcagaatt cggtcacact catcggcaag cttcctcttt
     2341 acgaccggat tgacggacag aatctccatg agttgccgac gtgtttcttc gaggtgccgt
     2401 tgaagttcgg agtccggata gctcttccga gccgcttcat cgaaacaccg atcaagatac
     2461 tgcgccagaa agacatagag acgcacaagc gccgcagcaa tgtcggtctg atctgtggag
     2521 gagagagcca ttacttcgat tccttcattc gttcgtcttg cccaatattt aaatgcaata
     2581 tattttcaat acatacaatg atttatcatt atcccccaca gtaaaacatt cacattatcc
     2641 gccagcgctg ctacccgtac aagctctgcc atgccttccg ccaaaagccg tacctgtcaa
     2701 ttgctgctga atggttgctt gacctttttg agcaaactat cgatggacgg accggctttt
     2761 ccggtacccc ctaccgcttc tacgattttc tttgcaatat cccgaaacgc cccggcctga
     2821 ggggatgccg gatcggctac gacgataggg tgaccggtat ccccaccatc ccgaatagcc
     2881 ggatcgattg ggacccgtcc gaggaacggc acaccgagct tggccgccgc ccgctcccct
     2941 cctccatgcg aaaagatctc cgtccgctca ccgcagtgac cacagacaaa gtaactcatg
     3001 ttttcgatga tacccagaag agggacattc actttctgga acatacccat ccccttgcgg
     3061 acgtcataga gagctacctc ttgaggcgtg gtcaccgtaa tcgccccggc caacggaacc
     3121 atctgtgaca aagagagttg cacatcgccg gtacccggcg gcaagtcgat gagcaagtag
     3181 tccaagtctc cccagagcac atcgcggaag aacgcttgca aatactgatg gaccatcgga
     3241 ccacgccaca caagcggagc ttcttccggc accagaaacg ccatggaaat cagcttcacc
     3301 ccgtaactct cgaccgggag aatcttgccg tctttctgtt ccggtccggt cgtgctcccc
     3361 atcatcatgg ggatattggg accgtagagg tcggcatcca gcagcccgac ctttgcgccg
     3421 gccacggcta atgcacaggc cagattcgcc gcaacggttg atttgccgac cccacctttt
     3481 ccgctgctca ccgcgatgac ctgcttcaca ccggggatca aattatcttt tgcctggggc
     3541 tgttgtgccc cgtgaccatg ttcatcagcc atatcgatat ctcctactct gattccgaat
     3601 cgactgcgcc ttagagagca tccacgatct aagctcccat ttagcccgac atcaacatca
     3661 tcataagcga tgccatacgc caaatgatat gggtcagtcg agctatcgtc cacgtaccga
     3721 ctggggaagt catagacgat gaccatcggc tgtctgcgcg gtatactgaa ttcaaacgga
     3781 gtgcatcacg ctcaggacta tcgatgacac cttctcactc gttggaaccg accatccgcc
     3841 tgatcggcga gcaactcgcc cggctctccg gcggcctatc accgtcgatc tttgacagcc
     3901 gctggtggtc ccattcggcg atcaacctgg cgatgaagga tgcgtcgttt aagacacgac
     3961 tgtttcactt catcgatgtc cttccggtca ttcaagacga tgaacgcgtc gtctcgctcg
     4021 ccgaagaata tttcggccgc gagagcagcg agttgttcgg ccttcagtgg ggattgaagg
     4081 cactggcttc aacgagcata ggcgcccgtc tcaccggaaa atccatcagg aagcaaatcg
     4141 aacagatggc caaaactttc attgccggtg cctcgatcga agaatcggtt ccggccttgt
     4201 cgggattatg gaaagacaga cgagcctggt ccgtcgatct cttgggcgag gctaccatca
     4261 gcgagcagga agccgaccgc tatcgtgacc agtgtctgaa cgccttgaat gaactcagtc
     4321 aggccaccaa gacttggccg tccattcctc tgctccaaca agaccacttg ggaccgatcc
     4381 cgcgcgtgca gctctcgctc aaaatttcgg ccctctcatc tcggctggac tccatcgatc
     4441 ccgatggaag ttacgagtcg gtcgccgcac gtctccgtcc gctggtggat ctggcacaat
     4501 cgctgccggc cggattgatt ttcgatatgg aacaggcgga gacaaaagac ctgatcttgc
     4561 atattttcaa gcaactcttc gcagaacctg cctatcgatc ctatccctat gccgggctcg
     4621 cgctgcaggc ctatcaccga gaaaccgaac gggatgtaca ggacctggtg gcttgggtga
     4681 gaacacgcca tgcgccgatc acgatccgac tggtcaaggg cgcgtactgg gactcagaca
     4741 ctgttcgata tcgacaagca ggctggccgt ctccgctttt tgagcataag gcggaaaccg
     4801 acattcacta cgagcggttg atctcggtgt tgtttgaaca ttccgatctt atccgtcccg
     4861 cgttcggcac gcacaatctt cgcacgttag ccgccatcga agcccatgcg gaatcgcggg
     4921 gcctgacttc tgatgcccgc gagtaccaaa tgatcttcgg catggcggaa cctttccagc
     4981 aagccatggt gaaactgggg cgtcgggtac ggctgtatac gcccgtgggc cggctgctcc
     5041 ccggtatggc ctatctggtt cgaaggctgt tggaaaatac gtcgaacgaa tcg
//
LOCUS       DATNDD010000459         2772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1517271, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000459 DATNDD010000000
VERSION     DATNDD010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517271"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VN647_11115"
     CDS             complement(<1..991)
                     /locus_tag="VN647_11115"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HWG97624.1"
                     /translation="MNEDMVESPPLSEPVITLPWERNRDVNAFLYLEWLVTNGLGGYS
                     SGTIMQIATRRYHGIFVPDLASPRGRTIIIPRLDEELDVGGTTVRLSGAEHADGRLDT
                     DIFKHLTAFRREWQTPTWKFSCHGREVHKRVIMPYGQNTVYVEYRLLAGEPVTLRLRP
                     FITFRMPDAPLSEARRGPFLLTVMRERYEVQVCEGVAPLKLGLRPHAGVFVMNSHISH
                     DVSYRIDRDRGSLHVEDLESPGYFTVQLRLDHPVAFVASMEPWENLQHNPDAIFDAER
                     ARLLKLIPQADAADPFETKLALAADQFIVFPGSRMEEHAFALASGEEARTVIAG"
     gene            complement(988..>2772)
                     /gene="treZ"
                     /locus_tag="VN647_11120"
     CDS             complement(988..>2772)
                     /gene="treZ"
                     /locus_tag="VN647_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWG97625.1"
                     /translation="GKTLSLGRNDETGYFTGIVSDASPGMTYRYRLDGDKDFPDPCSR
                     FQPEGPHGPSQIVNPSAHRWRDHDWPGVHMAGQVIYELHIGAFTEEGTFDAAVRELDE
                     LKRLGITLLEIMPIAEFPGRWNWGYDGVCLFAPSHIYGDYDALKRFLDAAHEKGLGVI
                     LDVVYNHFGPDGNYLSQFSDDYVTDRYPNEWGQALNFDGPGSEGMRELVIQNACYWIE
                     EFHLDGLRIDATHAIHDAGDQHILADLSVEARRVAGRRSIVLIAESEAQEIRAVRPVE
                     QGGWGLEAIWSDDFHHVSLVAATGRSEAYYTDYRGAPQEFVSAVKRGFLYQGQRYHWQ
                     GKARGTVVDQEPAGSFVFYIQNHDQIGNTLIGNRLHTLTSPGRYRVLTAINLLAPQTP
                     MLFMGQEYGASTPFLYFVDFGGHELGPKVRQGREEFLSQFTSYASDEAQKSMADPNDP
                     MTFERSKLDRAEQITHGPIYRLYRDLLRLRREDPVFSAQSRQNIDGAVLSSHAFALRY
                     FEETGYDRLVVINLGADLHFVPAPEPLLAPRPDGYWAFLWSSDHPQYGGPGIVNPLTD
                     EGWHISAESATVFRIEQDVAAVTYERTQ"
BASE COUNT          630 a          806 c          741 g          595 t
ORIGIN      
        1 atccggcaat gaccgtccgc gcttcctcgc cggaggccag cgcaaacgcg tgctcctcca
       61 tacgactgcc gggaaacacg atgaactggt ccgctgcaag ggcaagcttt gtttcgaagg
      121 ggtcggcagc gtcggcttga gggatcagtt tcaataatcg agcacgctcg gcgtcgaata
      181 tggcatcggg gttgtgctgg agattttccc atggctccat gctggccacg aacgcgaccg
      241 gatgatctag tctcagctgc acggtgaaat agccgggact ctcgagatcc tcgacatgta
      301 gggatccacg atccctgtcg atccgatatg acacgtcgtg gctgatatgc gagttcatca
      361 caaacacacc ggcatgcggt ctcaaaccga gtttcaaggg agctaccccc tcgcatacct
      421 gcacttcata ccgttctctc atgaccgtga gaagaaatgg tcctcgacgt gcctcactga
      481 ggggagcatc gggcatgcga aaagtgatga agggccgcaa tcgcaaggtg acgggctctc
      541 ccgcgagcag ccggtattcc acatagacgg tattctggcc gtacggcatg atgacccgct
      601 tgtgtacctc gcggccatga catgagaact tccatgtcgg agtctgccat tctcgacgaa
      661 aggccgtaag atgtttgaag atgtccgtgt ccaatctgcc gtctgcatgc tccgctccgc
      721 tcaaccgaac ggtcgtccca ccgacatcga gttcttcatc aagtctggga attatgatgg
      781 tacgtcccct cggcgaggct aagtcgggga cgaatattcc gtgataacgg cgcgtcgcaa
      841 tttgcatgat cgtgccggat gagtaccccc ctaacccgtt cgtcaccagc cactcgagat
      901 acaagaatgc attcacatcc cggtttcgtt cccaaggcag agtgatgacc ggttcggata
      961 gaggcggtga ctcaaccatg tcttcgttca ttgggtccgc tcgtaggtta ctgccgcaac
     1021 atcctgctcg atgcgaaaca ccgtcgcgga ctcggctgag atgtgccatc cctcatcagt
     1081 cagtgggttg acgatcccgg gtcctccgta ttgaggatgg tcgctcgacc atagaaacgc
     1141 ccaatatccg tcaggccgtg gagccagcaa cggctcggga gcgggaacga agtgaagatc
     1201 cgctcccaaa ttgatcacga caagacggtc ataacccgtc tcttcgaaat accgaagagc
     1261 gaaagcgtga gaactcagaa cggccccatc aatgttttgg cgtgactgcg cggagaacac
     1321 cggatcttcg cgacgcagcc gtaacaagtc tcggtagagt cggtagatcg gcccatgggt
     1381 aatctgttcg gcgcggtcga gcttggaccg ttcgaacgtc atgggatcgt tcggatcggc
     1441 catcgatttc tgcgcttcat cggaagcata actggtaaat tggctcaaga actcttcccg
     1501 tccttgtcgg accttgggac cgagctcgtg gcctccgaag tcgacgaagt agagaaaggg
     1561 ggtcgaggcg ccgtactcct gccccatgaa caacatgggc gtctgaggag ccaacaaatt
     1621 tatcgcggtg agaactcgat atcggccggg actcgtcaac gtatggagcc ggttacctat
     1681 aagggtattc ccgatttggt catgattctg aatataaaac acgaagctgc cggccggttc
     1741 ctgatcgacg accgtacctc tggccttgcc ctgccaatgg taccgctgcc cctggtatag
     1801 aaagccgcgc ttcacggcag acacgaattc ttgcggcgca ccgcgatagt ccgtgtagta
     1861 agcttcgctg cgccctgtgg ccgcaaccag cgacacatga tgaaaatcgt cgctccaaat
     1921 ggcctcaagg ccccacccac cctgttccac aggccgaacc gcacgaatct cctgcgcctc
     1981 actctcggcg atcaagacga tggatcgtcg tcccgcaacc cttcgtgcct caacggacag
     2041 gtccgctaaa atatgttgat cgccggcatc gtgaattgca tgcgtcgcat cgattctcag
     2101 accatcaaga tgaaattctt cgatccaata acaggcattt tgaataacga gttcccgcat
     2161 gccctccgaa ccgggcccat cgaaattaag ggcttgcccc cactcattcg gatatcggtc
     2221 ggtgacatag tcgtcgctga attgtgataa atagttccca tccggcccaa aatggttgta
     2281 caccacgtcc aaaatgactc ccagcccctt ctcatgggcg gcgtccagga agcgcttgag
     2341 ggcatcatag tccccatata tatgtgatgg agcaaacaga cacaccccgt cgtatcccca
     2401 attccaccga ccggggaatt cggcaatcgg catgatctcc aacagtgtga ttcccaaacg
     2461 tttgagctca tccaactcac gaacggcggc gtcgaacgtg ccttcctccg tgaaggcccc
     2521 gatatgaagc tcatagatga cttgtcccgc catgtgaacg ccgggccaat cgtgatctcg
     2581 ccaccggtgg gcggaggggt tcacgatttg agaagggccg tgaggtcctt ccggttgaaa
     2641 gcgtgaacaa gggtcaggga aatccttgtc tccatctaat cgataacgat aggtcatgcc
     2701 cgggcttgca tctgagacaa ttccggtaaa gtagcctgtt tcatcattcc tccccagcga
     2761 tagagtctta cc
//
LOCUS       DATNDD010000460         2550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2811698, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000460 DATNDD010000000
VERSION     DATNDD010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811698"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VN647_11125"
     CDS             complement(<1..1467)
                     /locus_tag="VN647_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97626.1"
                     /translation="MSILRLLLWAMSWLGLLAIDPSTAAERHVSRVPLFDNLGTLHHP
                     ITTNSHEAQQYFDQGLRLVYAFNHEEAVMAFEEAAHLDPSAAMAYWGVALASGRNINA
                     AMDKAAERRAWDAVQKARSHRAHASPAEQAYIDAIGKRYDVNAHARPALDKAYADAMR
                     VVWRQFPNDPDAGVLFAEALMDLRPWDLWTTAGRPKPGTEELVSTLESLLTRFPDHPG
                     ACHYYIHSMEASPTPGRAVACAERLPGLMPGAGHLVHMPAHIYMRLGRYHEAAERNAH
                     AADVDRNYLAGRKLSGNYADAYYTHNLHFLWASLAMEGRRAEALKVARRLTGTITEDE
                     ARKDKWKELYLPTPLWSMIRFGQWDELLREPPPPKPLRLQQGIWRLGRGMALSASGRL
                     PGAEGEHLVLTGMAKRLGRDRTPEEKTERTLLKIAERLLAGELAMHRHKYDAGITSLG
                     MGACGRDPHRLSVLRRTARGITSAMFVRAGELVSRQCPK"
     gene            complement(1627..2289)
                     /locus_tag="VN647_11130"
     CDS             complement(1627..2289)
                     /locus_tag="VN647_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97627.1"
                     /translation="MAPRFNKEPINLIHRIPSQKQIDEARPVPAPYGQLLLDAGAACW
                     RKVVDLFAYVTRYEDEASPRVRKPLALPDRTMEHATIHHDDIQQNGKRVQGAESQPAT
                     KVTILRKPVQAPTLVQSEEVAELRSFLIGQQDEIARLTSHIQELTSLVSSQQQVLVHL
                     GKELEVGAFSTTSNGTAAASIKRSRIGRRKPTDKETPNPSQAQENEHPFSPQTEHRSL
                     NL"
BASE COUNT          513 a          732 c          733 g          572 t
ORIGIN      
        1 tttcggacac tgccggctca ccaactcgcc ggcgcgcaca aacatggcgc tcgttattcc
       61 tcgcgccgtg cgcctcaaaa ccgagaggcg atgagggtca cgtccgcatg ctcccatgcc
      121 taatgatgtg atgcccgcgt cgtacttatg gcggtgcatt gccagttccc cggccagcaa
      181 tcgttccgcg atcttgagca gggtccgttc ggttttctcc tccggagtcc gatcacggcc
      241 taagcgcttg gccatgccgg ttagtaccag atgctcgccc tccgctccgg gcaagcgacc
      301 tgacgccgat agcgccatgc ctctccccag tcgccatatc ccctgttgca gacgcagcgg
      361 cttgggagga ggcggttcac gcagcagttc gtcccattgt ccgaacctga tcatcgacca
      421 caagggagtg ggaaggtaca attccttcca tttatccttc cgcgcttcgt cctcggtgat
      481 cgttcccgtc agtcggcgtg ccaccttcaa tgcctcggcc cttcgcccct ccatcgctaa
      541 ggaagcccaa agaaaatgca gattgtgggt gtagtaggca tcagcatagt tgccgctcaa
      601 ctttcgaccg gccaaatagt tgcgatccac atccgcagca tgggcattcc gttcagccgc
      661 ttcatggtat cgaccgagcc tcatgtagat gtgggccggc atgtggacta agtgtccggc
      721 ccccggcata agcccgggca gtcgctccgc acaagccacg gctcggccag gggtcggtga
      781 agcttccatg ctatggatgt agtagtggca ggcccccgga tgatccggga aacgcgtcag
      841 aagcgattcc aatgtagaca cgagctcttc ggtgccgggc tttgggcgac cagccgtcgt
      901 ccataaatcc catgggcgta ggtccatcaa ggcctcagcg aacaagacgc cggcatccgg
      961 atcgttcgga aactgccgcc agactacacg catggcgtcc gcgtaggctt tatcgagggc
     1021 cggtcgcgca tgcgcgttga cgtcgtatcg cttaccgatc gcgtcgatat aggcctgctc
     1081 agctgggctg gcatgggcac gatgtgagcg agccttttgt acggcatccc atgcccgccg
     1141 ttctgctgct ttatccattg ccgcgttgat gttgcgaccc gatgcgagcg ccacacccca
     1201 ataggccatt gccgctgagg gatccagatg cgcggcttct tcaaaggcca tgaccgcctc
     1261 ttcatggttg aaggcgtaca cgagccgtaa accttgatca aagtattgtt gagcctcgtg
     1321 ggagttcgtc gtgatgggat gatgaagggt gccgaggttg tcaaaaagag gcacccgaga
     1381 gacatggcgt tcagctgccg tactcggatc gatcgccaac agacctagcc aagacatcgc
     1441 ccacaagagg aggcgaagga tgctcatgga cggctttgta gcagacgtga ccagcacctg
     1501 gctagagcac aggtgtgcgt gttgagggga tcactctcgc gggtagtagg attcgctctt
     1561 ttttacctgc gaagtattca agaagcggct ggagccaccg accaacattc gcagatcggg
     1621 ccaagcttac agattgagcg accggtgttc agtttgaggc gaaaacggat gctcattttc
     1681 ctgagcctga gaaggattcg gcgtctcctt gtcagtcggt ttcctccgac cgatacggct
     1741 tcgcttgata gaggcagccg ccgtgccgtt cgaggtcgtt gaaaatgcgc cgacttccag
     1801 ctctttgccg agatgaacga ggacctgttg ctgagagctg accaaggagg tgagctcttg
     1861 aatgtgtgag gtcagccgag caatctcatc ctgttgcccg atcagaaagg accggagttc
     1921 ggcgacttct tccgactgaa caagagtcgg tgcctggacc ggctttcgta ggatcgtgac
     1981 ttttgtggcc ggttgactct ctgctccctg gactcgcttt ccgttctgct gaatgtcatc
     2041 atggtggatc gtggcatgtt ccatcgttcg gtcgggtagc gcaagcggtt tacggacccg
     2101 tggggaggcc tcgtcctcat accgcgtcac gtaagcgaag agatcaacta ccttgcgcca
     2161 gcaagcggct ccggcatcaa gcaggagctg accgtaaggt gcgggcaccg gacgagcttc
     2221 atcgatctgc ttctgagacg gaattcgatg aatgagattg atcggttctt tattgaatcg
     2281 tggggccatc tgtccccttc atttggacct atcgtcgacc tatgatgtac ctactcctcg
     2341 ggaaggccca gagtcaaccc ttttattctg caagaggtaa aatatattcg gagtccttaa
     2401 ataattttag aatcgtgtca cgctcgagaa atgaaactgc gggagggcca tggctggaac
     2461 taacatcttc cccgtttccg aattgactgc ctccatcggc gcggtccacc cgctgagacg
     2521 ttgaaatgcc cggagcgggc tctcatgaaa
//
LOCUS       DATNDD010000461        10156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1083212, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000461 DATNDD010000000
VERSION     DATNDD010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083212"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VN647_11135"
     CDS             complement(<1..372)
                     /locus_tag="VN647_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97628.1"
                     /translation="MHVLLIEDNEDDAVLIRDVLSRQGGEPVTVDWADRLDTGLAQLA
                     HGPFDAILADLSLPDNHGLDIVSTLRRHRRDAPVIVLTGLDDAAVAERALRHGAQDYW
                     VKSRLSPDGLTRAIRYAIERHQ"
     gene            complement(376..810)
                     /locus_tag="VN647_11140"
     CDS             complement(376..810)
                     /locus_tag="VN647_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97629.1"
                     /translation="MNSIDILVVEDNDDDVVLIQAAFSEGKVINRLIIVRDGEEAMTY
                     LRKQDVYAAAPMPGIVLLDINMPKKNGFEVLADIKADPQLRALPVVMLTVSDREEDIV
                     RSYEQGACSYIRKPVSLTRFIAVVKEFELYWSLVSKVPNLNR"
     gene            complement(807..3242)
                     /locus_tag="VN647_11145"
     CDS             complement(807..3242)
                     /locus_tag="VN647_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG97630.1"
                     /translation="MPGRFPGLPTNAIIPPHVIDRSDDRRIPIYSSTVISGVIAALFA
                     AVFLLDVLTPLGVSVPVLYIVPLALTPLLQRREAPYWLAGAASAATLCDYFFFHSHID
                     FPNWLPLSNRMISVSLVWLMAVIIRRQRDMADHLAHTAALEAENHAHLVKEEALQRQA
                     DEIQDLYNRAPCGYHSLDATGRYIEVNDTELQWLGYRREDIVGQHRFRDLLTPPSARR
                     FDEVFPHFVAQGSIHDLELDLVGKDGSLLPVSLSATAIRDSESHYIASRSTIVDITER
                     RRTEEVLRQAHAALETQVRDRTAELAIANRRLEEQLEESRRTEEALRVSESRFRLTAD
                     HAPVLIWISDNTKACTWFNKPWLEFVGRRLEQEVGNGWVDNVHPEDVARCVGIYVDSF
                     DTRREFTMEYRLRRHDGAWRWILDHGIPRYEGDGSFAGYIGSCTDIHDRKRAEELQGR
                     LAAIVESSNDAIVSTSLDGTVRTWNEGAQRMFGYGADEMVGRSIRTIIPADRHAEETE
                     LLGRAARGERIEEYETIRIRKDRTVLDVSLTASPVRDPQGSLIGIAKIVRDITEHKKA
                     ETVLINKNKDLETLLYVTSHDLKEPLRAIESFSQIVLERYADRIDERGSDYLRRIVRA
                     TQRLDRLLNDILELSRAQRMLPPMEEVEAEILVKEALTRLDARIQETGAAVQIVYPLP
                     RLRVNRMWATQGLYNLLANAMKFTCDGHPPDIEIASYEAPASTIPMTGVVVKDRGPGV
                     PTRHAERIFMLFQRAVGRNVEGTGAGLTIVRQVAERHGGRAWVQPRAGGGSEFVITFA
                     NSTHNGNGGASAI"
     gene            complement(3484..4323)
                     /locus_tag="VN647_11150"
     CDS             complement(3484..4323)
                     /locus_tag="VN647_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWG97631.1"
                     /translation="MPLTTYNHVSIPSFMYGTAWKKEATTKLVLQAVEAGFTAIDTAN
                     QLVHYDEARVGEALLHLSKQGITRDKLFLQTKFTPINGQDHRLPYDARASIAAQVEQS
                     FAGSLAHLHTDYLDSYVLHGPYSRRGLGAEDWEVWTAIESLYDAGKTKMIGVSNITAD
                     QLTLLCMKAKYKPMVVQNRCYAAFGWDKEVREICRTHHMIYQGFSLLTANRDVFINPE
                     VRAMAARYEITLAQLIFRFAMQVGMLPLTGTTNLQHMKEDLQSDRLTLLSEEVRQMET
                     IGL"
     gene            4875..5732
                     /locus_tag="VN647_11155"
     CDS             4875..5732
                     /locus_tag="VN647_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWG97632.1"
                     /translation="MQPTFRPGTVRDAAACGQIGYEAFKAIAEQHGFPPDFPSADRAV
                     GLLSQILTRPDVYSVVAELDGQVVGSNFLWEGDTIAGVGPITVASTAQNAGLGRMLMK
                     SVLDRANARRQAGVRLVQAAYHNRSLSLYTKLGFQAREPLSTLQGLPLKMELPGYRVR
                     QATLDDLALCDALCRGVYGCDRRGEIAHAIADGTATVVEHNGRITGYTTGLGFFGHAV
                     GRTNDELKALIGAAPAFTGLGFLLPTRNMELLRWCLNHGLRIVQPMTLMSLGLYQKPA
                     CSFLPSILY"
     gene            complement(5738..6097)
                     /locus_tag="VN647_11160"
     CDS             complement(5738..6097)
                     /locus_tag="VN647_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97633.1"
                     /translation="MGPTKAIVKEQALYEALGGKLIKDGFTDRQDLEAYVNHHYLVLP
                     VVDKQGRPWLLDGKPVYCLRGTQYETVNDERVHLARCPDCGGMGIRADEFVVESDCIH
                     CTACGHEFDARLEMMET"
     gene            6967..7533
                     /locus_tag="VN647_11165"
     CDS             6967..7533
                     /locus_tag="VN647_11165"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HWG97634.1"
                     /translation="MHRTSRVLGRAVVLAGLGVVLDLFTLMSVPCARAESLNVKPGAW
                     EITTTTTTTGSKISPELSTRLKPDQRAKMEQVLKARDGKPMTMTTQFCLTKEDVSHDR
                     IIKEMEDEDSDGEAQCKVKVISKSSSRLVIDRMCPAPHASTTHFTIDAKTTESFVATG
                     DGEPAGSGKARMEIKGKWLGAGCEGIEE"
     gene            7607..8470
                     /locus_tag="VN647_11170"
     CDS             7607..8470
                     /locus_tag="VN647_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97635.1"
                     /translation="MRDMRPRIIMLRPILYALILLLAATPGLVSGESGHLISTTPDGK
                     IDWTTGIATAHGIGIPPKDSASPLQAKEMTRKAAWSVALRNLLEVVKGIHVDSTTTVS
                     NYVTTNDEVRTHVDGFVKGARLIREQEMPDGSVETTVEMTLGREFSKLVIPKVDPKTV
                     PLSRYQKKPTSPSKSYTGLVVDAKGLSVHPALAPRLLNQQGEEVYSVAYVDQQATTKH
                     GIVLYEPDLASAQSNPLVTNTPLTIKALRAEGVDLYISDADAQTLHGVPSYFKLLRQA
                     KVLVILDQHNP"
     gene            8492..9397
                     /locus_tag="VN647_11175"
     CDS             8492..9397
                     /locus_tag="VN647_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HWG97636.1"
                     /translation="MQFGGLIAILPGLIMLFGYPIMVGLSDVAYAEGGKGLESVRGHA
                     CYGFGDDQTPAQARRGAMIKAQEDAVRNHGVFVKSSSRIKNFQLEEDIIQTTSAAMLQ
                     EVTVEKEERKPQELCITLRAKISPISVEDMIKQRIAAKEIAQEARTTLVPAQPKFGLK
                     IWTNKPNGHFTEGEKLIVYVQSERDAYLKLDYFQADGTVTHLVPNLYRGQAFITGGKT
                     YSFGDETSPEQYIINEPYGVEAIKAITGVQPFEAAADEADATGDSRNYLGRLRGIKVV
                     AAASSVELNTESRAVADYKKESPKR"
BASE COUNT         2263 a         2876 c         2839 g         2178 t
ORIGIN      
        1 ctgatgccgc tcgatggcat accggatggc gcgcgtgagg ccgtcggggc tcaatcgcga
       61 cttgacccaa taatcctgcg ctccatgccg gagtgcccgt tcggcgactg ctgcgtcgtc
      121 cagccccgtc aggacgatca ccggagcgtc gcgcctgtgc ctgcgcagcg tactcacgat
      181 gtccaggccg tgattgtcgg gaagggacag atcggccagg atggcgtcga aggggccgtg
      241 ggccagttgc gccaatcccg tatccagccg gtccgcccag tccacggtga ccggctcgcc
      301 gccctgccgt gacaggacgt cccggatcaa gaccgcatcg tcttcattgt cctcgatcaa
      361 cagtacatgc atcggtcacc tattgaggtt ggggactttt gagacgagac tccaatacag
      421 ttcgaactct ttcacgacgg cgatgaatcg cgtcaagctg acaggtttgc gaatgtacga
      481 gcaagcacct tgctcgtacg atcggacgat gtcttcttct cgatcgctga cggtcaacat
      541 gacgaccggg agggcacgta attgtggatc tgccttgatg tcggccaaca cttcaaagcc
      601 gtttttcttc ggcatgttga tatccaacaa tacgatgccg ggcatgggag ccgcggcata
      661 gacgtcttgc ttgcgcaggt aggtcatggc ttcctccccg tcgcgcacga tgatgagtcg
      721 gttgatcacc ttaccttccg agaaggctgc ctgaatgagg accacgtcgt cgtcattgtc
      781 ctccaccacg agaatgtcga tcgaattcat atcgccgatg cccctccatt gccgttgtga
      841 gtcgaattag cgaaagtaat cacgaactct gatccgccgc cggcgcgtgg ctggacccac
      901 gctcgaccgc cgtgacgttc ggccacctgg cgtacgatgg tgagtccggc gccggttccc
      961 tccacgtttc gtcccaccgc ccgttgaaac agcatgaaaa tccgttcggc atgccgagtc
     1021 gggaccccgg gacctcggtc cttcacgacg actccggtca tgggaattgt agaagcggga
     1081 gcttcgtatg aagcgatctc aatatccggc gggtggccgt cgcaagtgaa cttcatcgca
     1141 ttcgccagca ggttgtacag gccttgtgtg gcccacatcc gattcacgcg caaccgcggc
     1201 agagggtaca caatctggac tgccgcaccg gtctcttgaa tgcgggcgtc cagccgggtc
     1261 agcgcctcct tcaccagaat ttcggcctcc acttcttcca tcggcggaag catccgttgg
     1321 gcacgcgaga gctccagaat atcgttcaag aggcggtcca gccgctgtgt cgcccgcacg
     1381 atgcgtcgga ggtaatcact gccgcgctca tcgatccgat ccgcgtagcg ttccagcaca
     1441 atttgggaaa aactctcaat ggccctgagg ggttctttca agtcgtggga cgtcacgtac
     1501 aagagcgttt cgagatcctt attcttgttg atgaggactg tctcggcctt tttgtgctcg
     1561 gtaatatcgc gcacaatctt ggcgatgccg atgagacttc cctgcgggtc gcggaccgga
     1621 gaagcggtca gggagacgtc gagtaccgta cggtccttcc tgatccgaat tgtttcatac
     1681 tcctcgatgc gttcgcccct tgccgctcgt ccgagcagtt ccgtttcctc cgcatggcgg
     1741 tcggcgggaa tgatggtgcg aatggatcgg ccgaccattt catccgcgcc gtagccgaac
     1801 attcgctgtg cgccctcatt ccaggtgcgg acggttccgt ccagggaggt gctgacgatg
     1861 gcgtcgttcg acgactccac aatcgccgcc agccggccct gcagctcctc ggcgcgcttg
     1921 cggtcatgaa tatcggtgca ggaaccgata tagccggcga aggaaccgtc tccttcgtag
     1981 cggggaatgc catggtcgag gatccagcgc cacgctccgt cgtgccgccg gagccggtat
     2041 tccatcgtaa attcgcgtcg ggtatcgaaa gagtcgacat agatgccgac gcatcgagcc
     2101 acgtcctccg gatgaacgtt gtccacccat ccgtttccga cttcttgctc caggcgccgg
     2161 ccgacgaatt cgagccacgg cttgttgaac caggtgcagg ccttggtgtt atcgctgatc
     2221 caaatcaaga caggcgcgtg atcggccgtg agacggaagc gactctcgct cacccgtagc
     2281 gcctcttccg tccgccggct ttcttcgagt tgttcctcca agcggcggtt ggcaatggcc
     2341 agctcggctg ttcgatcccg gacctgggtc tccagcgcgg cgtgagcctg ccgcaacacc
     2401 tcttccgtgc gccggcgctc ggtgatatcg acgatcgtgg atcggctggc gatgtaatgg
     2461 ctctccgaat cccggatcgc cgttgcgctc agcgaaacgg gcaggagcga accgtctttt
     2521 cccacgaggt ccagctccaa gtcatggatg gatccctggg cgacgaaatg tgggaatact
     2581 tcatcgaacc ggcgggcgct cgggggagtt aaaagatctc gaaagcggtg ttggccgacg
     2641 atatcctcgc gccggtatcc aagccattgc aactccgtat cattgacttc aatgtaccgg
     2701 cccgttgcat ccagagagtg atatccgcaa ggcgcccggt tgtacagatc ttgaatttcg
     2761 tccgcttgtc tctgaagagc ctcctccttc acgagatggg cgtggttctc cgcctcgagc
     2821 gcggccgtat gggcgagatg gtcggccatg tcccgctgtc gtctaataat caccgccatg
     2881 agccatacca gagacacact aatcatacga ttgctgagcg gcagccagtt tgggaagtcg
     2941 atgtgcgaat ggaagaagaa gtagtcgcac aacgttgcgg cagaagctgc cccggcgagc
     3001 cagtacggag cctcgcggcg ctgcaataag ggcgtcagcg cgagaggcac gatgtaaagg
     3061 acggggacag acactcccag cggcgtaaga acatccaaca gaaagacggc ggcaaagagg
     3121 gcggcaatca caccgcttat caccgtcgag ctgtagatgg gaatgcgcct gtcgtcactc
     3181 ctatcgatca catgaggggg aatgatcgca ttggtcggaa gcccaggaaa gcgccccggc
     3241 atcttcatgc aggcctttgc gacagtgtta cgcgcggttt cgaccgacgg atggcttctc
     3301 gtcggccccg ctgcaaaccg aaagcacccc gatagatccg agagtatgct tgaaaaacaa
     3361 aggaaaattc agatgcgttc agccctggtc aatggctgta ccgcgtaggc tctttcagtc
     3421 ctagtaagag tacctatggt tccggggtga tccgactcca tgccgaggca ggggagaatg
     3481 gggctaaagg ccgatggttt ccatctgacg aacctcctcc gagagcaagg tcaatcgatc
     3541 cgattgcagg tcttctttca tatgctgaag attcgtcgtg ccggtcaaag gcagcatgcc
     3601 gacttgcatg gcaaagcgga aaatgagctg ggccagggta atctcatatc gcgccgccat
     3661 ggctcggacc tcgggattga tgaacacgtc gcgattcgcg gtcaggaggg aaaacccttg
     3721 atagatcata tggtgcgtcc ggcaaatctc ccgtacctct ttgtcccatc cgaaggctgc
     3781 gtagcagcga ttctgcacca ccatcggctt gtacttggct ttcatgcaga gcaaggtcag
     3841 ctgatcggcc gtgatgttgc tgactccgat catcttcgtc ttgccggcat catagagaga
     3901 ttcaatcgcg gtccaaactt cccaatcctc agcccctagg ccccgccgtg aatagggccc
     3961 atgcaacacg tacgagtcaa gatagtccgt atggagatgg gccaaggaac cggcgaaaga
     4021 ttgctccacc tgtgcggcga tacttgctcg cgcgtcgtat ggaaggcgat gatcttgacc
     4081 gttgatgggt gtgaacttcg tttgcagaaa cagcttgtcg cgagtgatgc cttgcttcga
     4141 caaatgcagc agcgcctccc cgacccttgc ttcatcataa tggacgagtt gattcgccgt
     4201 atcgatcgcg gtgaatccgg cttccaccgc ttgcaggacc agctttgttg tcgcctcctt
     4261 cttccacgcc gtgccgtaca taaaggaggg aatggaaacg tgattgtatg tggtcaatgg
     4321 catcgcaaac ctcctgtcag accttaatac catacacagt tcacaccggc ggtctcaacg
     4381 tgagaattgt aacgccacat gcatcctcaa gctccacctt tacatcatga ggcgatcacc
     4441 gagatccctc ggagccaacg gtatgtgagc agaccgaagc tcacgaagct gtttgagggg
     4501 aagcgcaccc aggccacgcg agacacggca attgttcagg ggtccatcgg tacggataca
     4561 gccagagaga ggttgcggag gtagtggggc tacactacgc gaccgtcagc cgaatcgcaa
     4621 acagaccgtt atacaaaaca caagacttga ccccataatt taccataatt taactagtcg
     4681 ctcgtcaacc cggagagctg tctgccggag ctctgccgct atgtcgtgct caatccggtg
     4741 cgagccagac agcgctgtac cgcagctacc ttcctgtcca gtggttctcc agtttctcgg
     4801 aaagccttac gccatgatct gccaattgac tcgtctcccc gtgcaacggt aacttcattc
     4861 ttaaaggata acccatgcag ccgacgttca gaccgggtac ggtgcgggat gctgcagcat
     4921 gcggacaaat cggctacgag gcattcaagg cgattgccga gcagcatggc ttcccaccgg
     4981 atttcccatc tgctgatagg gcagtgggac tgctctcaca gatattgacg cgtcccgatg
     5041 tgtattcagt ggtcgctgag cttgacggtc aagtcgtcgg cagtaacttt ctctgggaag
     5101 gcgataccat tgcaggggtg ggaccgatca ccgtcgcttc gaccgcccag aacgccggtc
     5161 ttggacgcat gttgatgaaa tccgtgctgg accgtgccaa cgctcgccgt caagccggtg
     5221 ttcggcttgt tcaggccgct tatcataacc gttcactgtc cttgtacacc aagctcgggt
     5281 tccaggcgcg agagccgctt tccactctgc aaggtttgcc gctcaagatg gagctgcccg
     5341 gttatcgggt ccgccaagcg accttggacg atcttgcgtt gtgcgatgca ttatgccgcg
     5401 gtgtgtatgg atgtgaccgc cgcggcgaga ttgcgcatgc cattgccgac ggcacagcaa
     5461 cggtcgttga acacaacggc cggattaccg gctataccac gggtcttggt ttctttggcc
     5521 atgcagtcgg tcgaacgaac gacgaactca aggctctgat tggcgctgcg ccggctttta
     5581 caggactagg gttcttgctg ccgaccagga acatggaatt gttgcgctgg tgcttgaatc
     5641 acgggctacg aatagtccaa cctatgacgc tcatgagtct cgggctctat cagaaacctg
     5701 cctgttcctt tctcccttcc atcttgtact gaatgcacta cgtctccatc atttccaacc
     5761 tggcatcgaa ttcatgtccg caggccgtgc agtggatgca gtccgattcg acgacgaatt
     5821 cgtcggctcg aatccccatg cctccgcaat ccggacaacg ggcgaggtgg accctctcat
     5881 cattcaccgt ttcgtattgg gttccgcgaa ggcaatagac cggttttcca tcgagtaacc
     5941 agggccgccc ttgtttatcg acgaccggca ggacgagata atgatggttc acgtatgctt
     6001 caaggtcctg ccggtccgtg aaaccgtctt tgatcaactt gccacccagc gcctcgtaga
     6061 gagcctgttc cttcacgatt gccttcgtcg gccccatagc gacctcccat cgttaaagtt
     6121 cagaaacgat ccaatgattg atcatgaacg cacacccatc attccgaatt ccacagtctc
     6181 atgaggactc ccccaccctt gtgcccaccg ggatcgagtc gacgccaacc gtcatgcggc
     6241 tcgcctgctg atggtcggcg tttgtgaatg tacgtcaacc gatctgccgg caccttgcct
     6301 cgattcctgt cgctcctcgt tgtcattcga agcgagcagg cgtatgcacg caatcaacat
     6361 cacgattaat gctccaatgc taatcacaat cccccaatat ccccatgaca taagtcacac
     6421 tctcgcgcca agcgacgcga gtcctttgtg aggttgccga cacagatctc cgccgtggaa
     6481 tcgcatcaga cgtcggtgcg ttatccgact ttcttggtac atgagacggc agtgagcggt
     6541 ctgcgcgaac atggcatggt atgaggagag ggcccgtctg gtgagaaacg tacacccggc
     6601 aggtccagac cgttggaact gtgctccacg gaggaccata agcaccacct ccagaatgag
     6661 gcggcaatga aaagttactc tcagcttacg ctttggagaa ttagcaggtc aagggggctg
     6721 agtcataccg gagtgccgcc ccaatcgatt ctgtctgagt tcagatccac cgacctccat
     6781 gtgtcttctt cgcgatagac gagcttgcct gaacacagta aaatggggcg cttctttccc
     6841 tgaaggggat acgaccatcc ggctttcgct gaaagaaacg gaaagggatg aaggaaactc
     6901 cagactctag gaaggaaagc ggtcgaccgt ttctggggag gaccacactc attgaaagga
     6961 ggggtcatgc acaggacgag tcgggtcctt ggtcgcgccg tggtcttagc cggattggga
     7021 gtggttctcg acctcttcac gctcatgtcg gtaccctgtg cgcgagccga atccttaaac
     7081 gtgaaacccg gtgcgtggga gatcactaca accacaacca ccacgggttc gaagatctca
     7141 ccggagctat caacgaggtt gaaaccggat caacgggcca agatggagca ggtgctcaaa
     7201 gcccgcgacg gtaagccgat gacgatgacc acccaatttt gtctcacgaa agaggatgta
     7261 tcccatgacc gtatcatcaa agagatggaa gacgaagata gtgatggaga agctcagtgc
     7321 aaggtcaagg tcatctcgaa atcctcgtcg aggctggtga ttgatcggat gtgtcccgcc
     7381 cctcatgcct caacgacgca ctttactatc gacgccaaaa caacagaaag ttttgtggcg
     7441 accggagacg gcgaaccggc aggatcaggc aaggcccgta tggaaatcaa aggcaaatgg
     7501 ctcggcgccg gctgtgaggg aattgaagag tagccctgca tttttgaaca aaaggtgcgt
     7561 aatgccctgc tcagattata aagagagcgt cctaatggag gcgtctatga gagatatgcg
     7621 tccacgaatt atcatgctac gacccatcct gtatgcgctt atcctcttat tagcggcaac
     7681 accgggactc gtatccggtg aatcagggca tttgatctcg acgacaccgg acggcaagat
     7741 cgactggacg acaggaatcg ccacggctca tggaatcgga atccccccta aggattccgc
     7801 aagccctctg caggccaagg aaatgactcg caaggcggca tggagcgtgg cgctgcgtaa
     7861 cctcctggaa gtcgtgaagg gtatccatgt cgattcgaca accacggtca gcaactatgt
     7921 caccacgaac gatgaggtac ggacacacgt cgatggtttt gtgaaggggg ctcgactcat
     7981 cagagaacag gaaatgccgg atggatcggt cgagaccaca gtggagatga cgctcggcag
     8041 agagttctcc aagctcgtca ttccaaaggt ggatcccaaa acggttcctc tgagccgata
     8101 tcaaaagaaa cccacaagcc catcaaaatc ttatacgggg ctcgtggtgg atgccaaggg
     8161 actaagcgtc catccggctc tggccccccg attgctgaat cagcaaggcg aggaagtata
     8221 ttccgtcgcc tacgtcgatc aacaagccac cacaaagcat ggaattgtcc tctatgaacc
     8281 ggatcttgcg agtgcacaat cgaatcccct tgtcaccaac acacctctga cgattaaggc
     8341 gttgcgggcg gaaggcgtgg atctgtacat cagtgatgcg gatgcccaga cccttcatgg
     8401 cgtaccgagc tacttcaagc tcttgaggca ggccaaagtc ttggtcattc tcgaccaaca
     8461 taacccttaa cgcgagggca cgggaactgt catgcaattc ggaggtttaa tcgccatatt
     8521 gcctggatta atcatgttgt ttggctatcc cataatggtc ggtttgtccg acgtagctta
     8581 tgcggaggga ggaaagggtt tggaaagtgt gcggggtcac gcgtgttatg gattcggcga
     8641 cgaccagacg cccgcccaag ccagacgtgg tgccatgata aaggcacagg aagacgctgt
     8701 ccgaaatcat ggagtgttcg tgaaatcctc ttcacggatc aagaacttcc agctggaaga
     8761 agacatcatc caaacgacca gcgccgcgat gttgcaggag gtcaccgtcg agaaggagga
     8821 gcgcaaacct caagagcttt gcatcactct ccgagccaaa atctcgccga tttccgtcga
     8881 agatatgatt aagcagagaa ttgcggcgaa agaaattgct caggaggctc ggaccacgct
     8941 cgttccagct cagccgaagt tcggcctgaa gatctggacg aacaaaccga acggacattt
     9001 taccgagggt gagaaactca ttgtgtatgt acagtccgaa cgggatgcct atctcaagct
     9061 ggactatttt caagccgatg ggaccgtcac ccatctcgtg ccgaatctat accgaggcca
     9121 ggcctttatt accggcggca agacctattc gttcggcgat gagacgagcc cggagcagta
     9181 catcatcaac gaaccctatg gcgtagaagc catcaaggcc atcacgggtg tccaaccatt
     9241 tgaagccgca gcagatgaag ccgacgcaac cggtgacagt cggaattact taggccggct
     9301 gcggggcatt aaagtggtgg cggcagcttc ttcggtggaa ttgaatacgg aaagtcgcgc
     9361 ggtcgcagac tacaagaaag agtctcccaa gcgctgaccc ttctcttaga ctttcctgat
     9421 gggggcaagc cgacagtcct ccaattcatg cttggccacc acaccgcatt ctgagataac
     9481 aggaaccaag agctgatctc tcagcttcac tccctataca tttccttgat cagtccaaga
     9541 gcgtgggcgc ttgctgaaca gagtagacgc gagaagtttt tctagcggtg gttcgtgcat
     9601 cagcggagtg ccacccgcgt cctcgcgccc agagggggac gaatcgggcc agtcggagca
     9661 acgaacggtg ggcgatggat gtgacgcctc ttctctatgg ccaggccggc tgggctcatc
     9721 tggcggccgt gatggattgt catgatcgcg aagtcatcgg ctacgagttg gcgttgcaca
     9781 gtacaaccag gagcgcccac atcaagcgct gggctatcga agcccggtcc aataccgggc
     9841 gcaacaagcc acccaggtgg cttgatttta ggggggcact acaacttttt cggagattcg
     9901 cgagatgggg cttggcgccg atattgagaa aatcgccatg cgttgagata aaaagatacg
     9961 aatggatcta atagattcag accatccttc attgagtcag ttacttgagg cgtacgggat
    10021 cattatttct gtcccctgtt acgctacgaa ttctggaaga tcccctgccc ctggaatggc
    10081 cttgctctct ccacttgggt ataattccag tccagtcgtt gcgagacaca tcgtatggct
    10141 ccacagctca tagccg
//
LOCUS       DATNDD010000462         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1923952, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000462 DATNDD010000000
VERSION     DATNDD010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923952"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..1081)
                     /locus_tag="VN647_11180"
     CDS             complement(464..1081)
                     /locus_tag="VN647_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97637.1"
                     /translation="MQWLLDQYQQVHFRDYMAIQVSPFCGTTAFPERMGDSFPDLLAS
                     KRLVQGYNVSGPLKPDTCIAVDRDLRDSTWRGLFHSALVENRRFQRGLFDGTDITGRH
                     NDGHVEQTLMVRLKDVSFETTPFTVAGIRQLSRRRLIGREADLFDYGLALLKTSASLV
                     YTIQLATAEQLAAATDSRAHFTLLTRLIERMGFTIENGLVEQGMS"
     gene            complement(2261..>2759)
                     /locus_tag="VN647_11185"
     CDS             complement(2261..>2759)
                     /locus_tag="VN647_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97638.1"
                     /translation="PAALVVGPTGLVYDHKTNQLFVSSTADNAIFVVAGAGTTMMDLG
                     RGALVYMDSTHLHGPLALVRAPNGDFLVSNSDVINSDPAQPSEIVEFTREGQFRRQLS
                     VDLNQGGSFGLNVHRDFSTDTVQFAAVDDSTNALTIWTLPMPEGEFFEHREERDGRHT
                     GERSP"
BASE COUNT          665 a          709 c          765 g          620 t
ORIGIN      
        1 gattctgccg attatgtata cattgcagac atggttgtca cagacaaaat ccaccttcta
       61 tgctacttga gtcggctgct gtggagatct tgaaggagga tgaatggaga ctataaagca
      121 agtgttatgg gtgctagtgg atactggcgg atctgaaacc acttaccgag tatttgaaaa
      181 caaacctggt agccctgcct acatggaacg agagaaggct agacctactc cgatagatcc
      241 catatttcga tcgtacagag aaatagttga atatctgcgg tcgctcggat ttacaccacc
      301 accaagtcag tactttcatc aaactgatga agaggtaaga gacggaaaag ttgagtttgt
      361 acgtaaggtc acaaaggccg gtatagagta gttggccact ttgagaagcg aagttcgttg
      421 tgacgaatga cgaagtaggt tctagcagac ctgagtgtaa tggttaagac atgccttgtt
      481 cgaccaaccc attctcgatc gtgaagccca ttcgttctat aagccgagta agtaatgtga
      541 aatgggcacg cgagtcagtc gcagcggcca gctgttcagc cgtcgccagt tgtattgtat
      601 acaccagtga ggcggaggtt ttgagtaggg caaggccata gtcgaagaga tcagcttctc
      661 tgccgatgag ccgtcgccgg ctgagttgac ggataccggc caccgtaaag ggagtcgtct
      721 cgaacgaaac atccttcaga cgaaccatca aggtctgctc gacatggcca tcgttgtgac
      781 gcccagtgat gtctgtgccg tcgaacagac cgcgttggaa tcggcgattc tccaccagcg
      841 cgctatgaaa gagcccacgc cacgttgaat ctcttagatc acgatccacg gcgatgcagg
      901 tgtccggctt gagcggtccg ctgacgttgt acccctgcac cagtcgcttg cttgccagga
      961 ggtcggggaa ggaatctccc atacgttcgg gaaaggccgt tgtcccgcag aaaggactga
     1021 cttgtatggc catatagtcc cggaaatgca cctgctgata ctggtccagc agccactgca
     1081 aggtgcgctc gtggcacaga tggaaaggat aaaatatgac gccggatcgt gtcatgccag
     1141 tgattcagca tagctgctct gcaacagtat cggcaagctg tcgtcccagg ggcaatggaa
     1201 cggaagctgt gatattgcgc cacagcgatt ctgaccgttg gcaaatactg cgctgacgat
     1261 ccctggagac aaaacgtact ttgcgtagcg ggaacattct cagttactga ctgatagccc
     1321 gtgggcacgt tggaatgctt catgggtgtt gcgtgggttc agcatttccc gcgccgttcg
     1381 ttcctgatca ccattgaaca tatggcggtg tatcgcaccg gccgttccat tgcccctcgt
     1441 tggaaggact cctgtatgct gaggtgaaca tgagcgcggc aggataacag gtttatgtgg
     1501 caggcacgac gccgatgcta ttcagcgctg aggctgacat ctgagaggca cggttccaga
     1561 tctggtcaat aggaggagag gcgtgtgacg ggatatgaca gcttccgttc tattgaccta
     1621 gttatggcga acgttaatgc gacagggctg aggaccgctt tgcggcgagg tgatcacggc
     1681 gccgaacttc cgctcctggc cggtgagcga ccatcggtct ccgaccctta gccgaatgct
     1741 tgtagaaatc agcaagatgc atgccctcac cttcagaaag tgttcgttaa accatatcaa
     1801 cgtgacatca atgcgatgct cattacagca atcgtctggc gagtactaca tgtatccaat
     1861 tcgattcata tgaatcgaaa aagattcggc gatgagaaaa aagggcttgt atggactgac
     1921 cttcccgatc taaaccaagc tggtgctgga ggtgggcgtc tagccgacag aaggagacgg
     1981 tatggcacgg aaacggtatg tggcagagga gattatcgga catctgggga cgatcgagat
     2041 tggagttgac gtatagtccc catcagatcg tttcatgtga taggcggggt cacacatcgg
     2101 tgaccccgca gctcacctta tccttcaaat ccaagtcggt atagtcgatt ttgttcctca
     2161 ggtgcaccac cccagaacca cagaccaccc ctcagcaacg accgcaccgt tgccgtcgat
     2221 gtggagagaa atgacctgag gcctctcacc gatcagaggg ttatggagag cgctcccctg
     2281 tatgcctacc gtccctttcc tcccggtgct cgaagaactc tccctctggc atcggcaggg
     2341 tccagattgt caaggcattg gtactgtcat cgacggcagc gaattgcacc gtgtcagtgg
     2401 aaaagtcgcg gtgcacattc aacccaaacg acccgccttg gttgagatcc accgagagtt
     2461 gacggcggaa ttgtccctcg cgcgtgaact ccacaatctc actcggctgg gccggatcac
     2521 tattgatgac atcactgttg ctcaccagga aatccccgtt cggggctcgt actaacgcca
     2581 gcggcccatg caggtgggtc gaatccatgt acacgagggc tcccctgccg agatccatca
     2641 tggtggttcc cgcccccgcc actacaaaga tggcattgtc agccgtcgat gagacaaaca
     2701 gctgattggt cttgtggtca tacaccaggc ccgtcggtcc caccactaat gcggcgggg
//
LOCUS       DATNDD010000463         5433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_223624, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000463 DATNDD010000000
VERSION     DATNDD010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223624"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VN647_11190"
     CDS             <1..749
                     /locus_tag="VN647_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HWG97639.1"
                     /translation="LQWDMGWMHDTLKYMQTDPIYRHYHHQNLTFRMLYAFHENFVLP
                     LSHDEVVYGKRSLLEKMPGDDWQKFANLRVLFGYMYAQAAKKLLFMGGEIAQRAEWAH
                     DGQLEWEQLQHASHLGIQRWVADLNRVYRTERALHEDDVTPHGFEWIDCNDAESSVIS
                     LIRKGHTMQDIILVVCNFTPVPRSNYQVGVPRGGYWREILNSDSSWYGGGDWGNGGGV
                     EAAPVPLHARSHSLTVTVPALAALFFKSQG"
     gene            complement(926..1180)
                     /locus_tag="VN647_11195"
     CDS             complement(926..1180)
                     /locus_tag="VN647_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HWG97640.1"
                     /translation="MKLVFSEQAWEDYLYWQKIDKKLVQRINDLIKEITRTPHTGTGK
                     PEPLKHALAGYWSRRINEEHRIVYKVVDDSVLIAQLRYHY"
     gene            complement(1177..1428)
                     /locus_tag="VN647_11200"
     CDS             complement(1177..1428)
                     /locus_tag="VN647_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWG97641.1"
                     /translation="MDAITYSTARAKLADTMDRVCEDHEPIIITRSGQQAVVMMSLDD
                     FKALEETSYLLRSPKNAQRLLESIAALESGKGKVRSLAK"
     gene            complement(1871..2509)
                     /locus_tag="VN647_11205"
     CDS             complement(1871..2509)
                     /locus_tag="VN647_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97642.1"
                     /translation="MNATDQNMRRKVDERRLPAGPRSWVLLCSVSLMLGVGGCSLSYD
                     TTNTPRAATEQLVLAQAFQRSLIDAVVPVVRPGQSVAVETTGLTTDQGYASALIERWL
                     NREGFHVPKDGKETLVVRVTLDTFGTLHNETFFGMPALSSAFIPFALPQLTLYQATRQ
                     RGLARFSMDFLDKTTGRLVGSTPTYEGDAYYNKYIFLLVFSTTLSDLIPPVP"
     gene            complement(2596..3153)
                     /locus_tag="VN647_11210"
     CDS             complement(2596..3153)
                     /locus_tag="VN647_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97643.1"
                     /translation="MRTFMIACGWMMVFFAGCVPVTDGFRHDLPTKDVLTIVWGDDVS
                     AVGMATTWLQKRGLFVLERSLLYANLETDNLELTHTLKDEAAILQTAKKMGVQEVVFL
                     DRGGDSRAPMISVRGVEVQTGRVSWSGSARYATFETRPTKDALALLTCEALATVWGYR
                     PAGTKWFLSSEKMCAVDGALPTGSR"
     gene            3752..5191
                     /locus_tag="VN647_11215"
     CDS             3752..5191
                     /locus_tag="VN647_11215"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWG97644.1"
                     /translation="MDTKTAAELKSFLEIPYDDLEDMNLKAAERAEKAGAKDLEQEYT
                     DWLKKEKHIKAVTLCFSDIEGRLHMLDYDKKFLMDSLGNLTFDGSSIRGFTPQHESDL
                     RLAVDWSSIRYFPADVIGAGKVIFFASVLNSDRTPYHSDFRGMLKSFTEHLKKNESIT
                     AYAACEIEGFLVEGQNAEQRYGEHGLNLISTGGYYHSLPMDPLRQFIDKSAEAQRALG
                     FKNEKDHPEVAPSQFEMNFSYADAVRAADQVQLYKLICRQVARSMGHTATFLPKPFVG
                     INGSGMHTNFSLSKNGKNIFHDAKGKDGLSDVAWDFILKLLNHAPEICLVFNPSVNAY
                     RRLDPHFEAPNQIKVSAIDRGSMIRIPMANEKTARIELRSVAPDANPYLVLYTMLKTG
                     FEGERLEKEQPTGDRARFLPSSIHDAIVLFNESKFIADILGEDSKRKYSSFKQLVADR
                     SPKELGTMVKASEVLFHHEVTNQMLWNQF"
BASE COUNT         1211 a         1490 c         1511 g         1221 t
ORIGIN      
        1 gcctgcagtg ggatatgggc tggatgcatg acaccttgaa atatatgcag accgatccga
       61 tctaccggca ttatcaccat cagaatctca cgtttcgcat gctctacgcg tttcacgaga
      121 atttcgtctt gccgctgtct catgacgaag tggtgtatgg gaagcgttcg ctgctcgaaa
      181 agatgccggg agacgactgg cagaagttcg ctaatctccg tgtgctgttc gggtacatgt
      241 acgcgcaggc ggccaaaaag ctgctcttca tgggtggaga gattgctcag cgcgcggaat
      301 gggcccacga cggtcaattg gaatgggagc aactgcagca tgcgagccat ttaggtattc
      361 agcgatgggt tgcggatctc aaccgtgtct atcggaccga acgggccctg catgaggatg
      421 atgtgactcc ccatggattt gagtggatcg actgcaacga tgcggagtcg agcgtcatca
      481 gcctgatccg caaggggcat acgatgcagg acattatctt ggtggtgtgc aattttacgc
      541 cggtgccgcg ttcgaattat caggtagggg ttccgcgtgg gggatactgg cgggaaattc
      601 tcaacagtga ttcttcctgg tatgggggtg gcgactgggg caacggaggc ggcgtcgaag
      661 ccgcaccggt gccgctgcat gcgcggtccc attcattgac cgtgacggtt cccgccctgg
      721 cggcgttgtt tttcaagagc cagggctagt cttaaattct gaatgatgaa ttatggacgg
      781 aggttatgct taccggtatg cacagaacaa ggcttgaccc caattcaatt cataatccca
      841 atagcatcgt gtccttcgaa attatggcag gttcatgtgg gcggtcctag gcatcggcgg
      901 ctcaacgcca aggcgttagg taccctcagt agtgatatcg cagctgagcg ataagcactg
      961 aatcatcgac cactttgtaa acgatgcgat gttcttcgtt gatacggcgc gaccaataac
     1021 ctgccagggc gtgcttgagc ggctcaggct ttcctgtgcc tgtgtggggc gtccgggtga
     1081 tctctttgat aaggtcattg atgcgctgca ccagcttttt gtcgatcttc tgccagtaca
     1141 aatagtcttc ccacgcttgc tcggagaaga cgagtttcac ttggccaagc tccgcacctt
     1201 gccctttccg gattcaagtg cggcaatgct ttccagcagc cgctgtgcgt tcttggggct
     1261 acgcagcagg taagaagtct cctcgagcgc cttaaaatca tcgagcgaca tcattacaac
     1321 ggcctgctgg ccgctgcgtg tgatgatgat aggctcatgg tcctcgcaaa cgcggtccat
     1381 ggtgtcggcc aatttggctc gggcagtgct gtaggtaatt gcatccatgg cgcctccttt
     1441 gtacaaatat ttgtacaaag gaaagcctgt agtgtcaagc gcttgccagt gacgagcgac
     1501 gcttcggcct tgcagaaagg gcgctttcga cggggatacc gagatcactg tcgcatgccg
     1561 agatctcggg gactcgtaac cctcctggaa aatgcaactg ccgattaagg actggcactc
     1621 gccgtctaat gtagcctgaa acgtcgttcc atctgatgcc tgcagggtgg aaacctatat
     1681 gattacaaga cccgccatct cgtgcttgat tcccttcaag tcaatgctca ccggtatgca
     1741 cagaacaaga ctcgatctca attccacttg cgagagctgc ggggaaatgt ttctgcgcgc
     1801 ttgaatcaac cgccgggggt catggtcgaa gcgcagtccc actcgatgaa cgcctcatga
     1861 ttgatcgcat ctatgggaca ggcggtatca gatccgacaa tgtcgtcgag aagacaagca
     1921 gaaatatgta cttgttatag taggcatcgc cctcgtatgt tggtgtcgag ccgacaagcc
     1981 gcccggttgt cttatccaga aaatccatcg agaaacgggc aagtcctcgc tgtctggttg
     2041 cctggtagag agtcaattgg ggcaaggcaa atgggatgaa cgcgctgctg agcgccggca
     2101 taccgaagaa cgtctcattg tgaagcgtgc cgaacgtgtc caacgtcacc ctgactacca
     2161 gggtttcttt tccgtctttc ggcacatgaa acccttcccg gttcagccat ctctcaatta
     2221 acgcactcgc atacccctga tcagtcgtca ggccggtcgt ctcaaccgcg acagattgtc
     2281 ctggacgaac gacaggaacc accgcatcga tcagactcct ttggaacgcc tgggccaaga
     2341 ccaattgttc ggtggcggct ctgggcgtgt tggtcgtgtc gtaggagagc gaacaccctc
     2401 cgactcccag catcaagctc actgagcaga gtaagaccca gctccgcggt ccggccggca
     2461 gccgccgctc gtcgaccttg cgcctcatgt tttgatcagt tgcgttcatg cgaagacctt
     2521 tcacgcggtt catcggaagg tggatcgatg atccgcctct cttgctgctg cctcttatga
     2581 atcgtccgag ccgactcatc gcgatcccgt cgggagagct ccatcaacgg cgcacatctt
     2641 ctcggaagag aggaaccact ttgttcctgc cggacggtac ccccataccg tcgcgagcgc
     2701 ctcacacgtc agcagagcga gggcatcctt cgtaggacgg gtctcgaacg ttgcataccg
     2761 ggcgctgccg ctccaggaga ctcgtccggt ctggacctcg acgccccgga cgctgatcat
     2821 cggagcccga gaatcgcctc cgcggtcgag aaataccact tcctgcactc ccatcttctt
     2881 cgcggtctga aggatggccg cttcatcctt gagcgtgtgc gtcagttcga ggttgtctgt
     2941 ctccaggttc gcgtacaaga ggctccgctc gagaacaaac agccctcgtt tctgcagcca
     3001 ggtcgtcgcc atgcccacgg cactcacatc gtctccccaa acaatcgtga gcacgtcctt
     3061 cgtcggcaga tcgtgtcgga acccgtcggt caccggcacg cacccggcga agaataccat
     3121 catccagcca cacgcgatca tgaatgtcct catgaaaccc tccgtacaac tctcagtatc
     3181 cgacggttca atttcctcgg tcgcctcgag aaagcccaga cttccggcat aaatggggtc
     3241 agatcttgca tcgtgtatgt tgttttcaga tatgctgcag agtgttctta tatttccaat
     3301 tgctgagcat atgctacgca cataaggcgt tttgacgaga aagaaatact gggaatgtcc
     3361 ctagtcgtgg ggtcgagctc gcgcacttca tttgctcgac cggatcaccg gcaaagtagt
     3421 tgttgcgagt gagctaaaca gcagatagcc ggttcgcacg agagtcccat cgtcgagatt
     3481 caggccgacc ggtgttcatg tatcgccgtg gcgaccacag ggttggcttg acggtccgcg
     3541 acggtccggt tcgaatgaac atggaccgcg ctcattcctc caaatctaca ttgcatgcac
     3601 aagcaaggtg tgccagactc gtttccgccg gatgatcgct tcttgctcgt ctctggtaaa
     3661 gatggtcgtg aaggcaacaa gcagtcaggc gatgtgagcc ctgagtgcat atgtccacac
     3721 ccttggatac tacgaattgg tgaggtgagc catggataca aagacagcgg ccgaactgaa
     3781 gagttttctg gagattccgt atgacgatct cgaagacatg aatctcaagg cggccgagcg
     3841 cgccgagaag gccggcgcaa aagatctcga gcaggaatat acggactggc taaaaaagga
     3901 aaagcacatc aaggccgtga cgttgtgctt ctccgacatc gagggacgtc tccacatgct
     3961 cgattacgac aagaagtttc tgatggactc gttgggaaat ctgacgtttg acggatcgtc
     4021 catccgtggg ttcacgccgc aacatgagtc ggatctgcgt cttgccgtcg attggtcatc
     4081 catccgctac tttccggcgg acgtgatcgg ggcgggaaag gtgattttct ttgcgtccgt
     4141 cttgaacagc gatcgtacgc cgtatcatag cgactttcgc ggtatgctga aatcgtttac
     4201 ggagcacctc aagaaaaacg aaagcatcac ggcatacgct gcctgtgaaa tagaaggctt
     4261 tctggtggaa gggcagaacg ccgagcaacg atacggtgag catggactga atctcatctc
     4321 aaccggcgga tactaccact cactcccgat ggatccgctt cgccagttta tcgacaagtc
     4381 ggcggaagct cagcgcgcac tggggttcaa gaacgaaaaa gatcatcccg aggtcgcgcc
     4441 ctcgcagttc gaaatgaact tttcctatgc cgatgcagta cgcgcggccg atcaggtgca
     4501 actgtacaag ttgatctgcc gccaagtggc ccgatccatg ggacataccg ccacgttcct
     4561 tccgaagccg ttcgtcggga tcaacggctc cggcatgcac accaacttct cgttgagcaa
     4621 gaacggcaag aacatttttc atgacgcgaa ggggaaagac gggctttccg atgtggcctg
     4681 ggacttcatc ctcaaactct tgaaccatgc gccggagatt tgcctcgtgt ttaacccatc
     4741 ggtcaatgcg tatcgccgtc tcgatccgca tttcgaagcg ccgaatcaga tcaaggtttc
     4801 ggcgatcgat cgcggctcca tgatccgcat cccgatggcc aacgaaaaga cggcgcgcat
     4861 cgagctgagg tcggtcgctc ccgatgcgaa cccctatctc gtgctgtaca cgatgctcaa
     4921 gaccggtttc gaaggagagc gcttggaaaa ggaacagcca accggcgacc gcgcgcgatt
     4981 cctgccgagc agcatccatg acgctatcgt gctctttaat gagtcgaagt ttatcgccga
     5041 tattctcggc gaggacagca aacggaaata ttccagcttc aagcaattag tggcggatcg
     5101 gtcgccgaaa gaactcggca cgatggtgaa ggcttcggaa gtcctcttcc atcacgaagt
     5161 tacgaaccaa atgctctgga atcagttcta gaggaacgag acaagctaag tcagtgggta
     5221 ggtcgaaacg gtgagtatct gaaaatcgaa ggccctctca gacgtgcgat tcgtacatcc
     5281 gttagaacaa gcagggggcg gcgtccttgg acggacgccg ccccctgtga gtctgacgca
     5341 cgggagtgag ctagagccag gtcacccgtt cggcgggccg gatgtagatc ggctcttcaa
     5401 cctgaatccg cgcgatttcc ttgcccgact tgt
//
LOCUS       DATNDD010000464         5227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2801605, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000464 DATNDD010000000
VERSION     DATNDD010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801605"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VN647_11220"
     CDS             <1..221
                     /locus_tag="VN647_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97645.1"
                     /translation="ISPVIGTSTAGGNTNVTTKVSLGVGMASALSVHVGNEGATLMSQ
                     MSNSNTTTQLASVGDFYSRFVSWVHQRD"
     gene            336..1295
                     /locus_tag="VN647_11225"
     CDS             336..1295
                     /locus_tag="VN647_11225"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HWG97646.1"
                     /translation="MAVKSWFLALGVCALSINASCTESSTGTANGSAPAKGSQAPTIT
                     AAKILNDPIPLTGPVEVQVDALDPEREAVSFYYQWHVNNIPFTGQTNATLPAELLKRG
                     QTVSVEITPSDGANRGQPYRTKSVVVGNTAPKVSAVSLSPQIARSGDKLEAMVEASDP
                     DHDRVDLTYKWYRNESVIKEGEEPFLNTTGFVARDKITVEVTAHDPAATGNSLKSEPL
                     VLENSAPKIVSTPPISDAQDRFNYAVKAMDLDGDQLTYYLEAAPTGMSISETSGHILW
                     QVPSDQKGIFHVKVVAKDGHGGMATQEFDLTLTSTGPAKPSGT"
     gene            1385..2452
                     /locus_tag="VN647_11230"
     CDS             1385..2452
                     /locus_tag="VN647_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWG97647.1"
                     /translation="MRKLKLREGTNTAILFGHHDRHLKLIEDELGVRLSARGEELTLD
                     GLPEAVRQAERILVELASLTNQGISLQAEDVTHVLSALRRAPEASLKDVLGQSAMIVT
                     KKRFVGPKSPTQKAYIDAIEQHDIVIAIGPAGTGKTYLAMAMAVSALINKEVSRIILA
                     RPAVEAGEKLGFLPGDIYEKVNPYLRPLYDALFDMMDMERANRLIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFNSKVVVTGDITQVDLPSDRVSGLIQV
                     KEILRDIEGIEFVYFDEKDVVRHRLVQDIIKAYDRHQPVNSDDPRHARGQVTSHQRPS
                     PNSLKSPEARSSPPGLTSSSH"
     gene            2452..2946
                     /gene="ybeY"
                     /locus_tag="VN647_11235"
     CDS             2452..2946
                     /gene="ybeY"
                     /locus_tag="VN647_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWG97648.1"
                     /translation="MAVYLRVSLTRFAVRQSTLAHLAEHVLSVVGESRSELSLELTGD
                     SRMRRLNLEYRKNDYPTDVLAFPIREAVMPRGVRPLSPMLGDVVISLPTAVRQAREAG
                     RSIDDELAILLVHGVLHLCGYDHERSAREAARMLRRERALLHLISPVPRMVTFRAGRK
                     PRGR"
     gene            2949..3884
                     /gene="ftsY"
                     /locus_tag="VN647_11240"
     CDS             2949..3884
                     /gene="ftsY"
                     /locus_tag="VN647_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWG97649.1"
                     /translation="MGWFQRLSEGLGRTRNVVQQSLDRFLGRAPDEELLEDLEAALLA
                     ADLGARVVDRLMLQVRGETRGADANTSEGLQNVLSRSLYSILKTVSGPSIDQLVNESS
                     KPLVTLVVGVNGVGKTTTIAKIAQRMTQAGRRPLLVAGDTFRAAAIDQLQVWGNRIGV
                     EVIHQRHGADPAAVAFDGIVAAKARTVDMVLIDTAGRLHTKSNLMDELRKVKRVIAQE
                     LPGAPHEVLLVLDATVGQNALAQARQFHEAVGVTGLALTKLDGTARGGIVVAIAEELK
                     LPLRLIGVGEGADDLQDFNAEAFVAALFGQPTSHS"
     gene            3971..4756
                     /locus_tag="VN647_11245"
     CDS             3971..4756
                     /locus_tag="VN647_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97650.1"
                     /translation="MIKVLIVDDDQMNCDLLQSVFTRQGCHVISTTSGREGLDLFRAH
                     NPRVTLLDLRMPEMDGLTVLKEIRAIDPHAPVIILGGGATEIQENQARALRATDFIRK
                     GLSLDVLVEAVNQVSKLPVPSTTAQPALGRGNVIEQIDESVLVVDDEPLLCDLLVRFL
                     SLRGYRAFGVKDGHEALRIVEDIPPDAIVLDLIMPGIQGIEVLRTLRDKCYSGGIIIM
                     TGSHNEELLREAWSLGPQEILIKPVDLERLLTAIQLVLVCREC"
     gene            4768..>5227
                     /locus_tag="VN647_11250"
     CDS             4768..>5227
                     /locus_tag="VN647_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97651.1"
                     /translation="MSIRKYLLRSSLISLLSISMVHAWGVSVSSAQENAANIQHTLSA
                     ELILAAHELVANDQIKLEVEPEVTSVTFTLAPTLHVESIAMQTHSVSGGQGNQDEVVP
                     FTSGYSSEATVQHVVVSLPKEHGRRVTLVMSYRGQINDPPREPRHLRFVTP"
BASE COUNT         1212 a         1378 c         1420 g         1217 t
ORIGIN      
        1 ccatatcacc tgtgatagga acttctacgg cgggtggcaa taccaatgtc accacaaaag
       61 tctcactcgg cgttggcatg gcttcagccc tctcggtcca cgtgggaaat gagggtgcta
      121 ccttgatgtc ccagatgagc aacagcaata caacaaccca gcttgccagt gtaggagact
      181 tctacagccg atttgtcagt tgggtgcatc aacgggacta aaatgcccgt gatgaggatg
      241 tgtaaacaga tcatggcgcg gacaatctac gtcatgatct gattaatgat tggaacaatt
      301 aaatcctagt gaacaacgta cgcaggttgc tcacgatggc cgtcaaatcc tggttcctcg
      361 ctcttggagt ttgtgcgctt agcattaatg cttcctgtac ggaatcttct accggcacag
      421 ccaatggctc agctcccgct aaaggtagcc aggctccgac catcactgct gcaaagatcc
      481 ttaacgatcc tatcccactc accggacctg tggaagttca ggtcgatgcc ctagatccag
      541 agcgagaagc ggtttccttt tattatcaat ggcatgtcaa taatattcct ttcacaggtc
      601 agaccaatgc taccttgcca gctgaattgc tgaaacgtgg gcaaacggtt tctgtagaga
      661 ttactccttc agacggtgca aacagaggcc agccctatcg aacaaagagc gtagtggtgg
      721 gaaacacggc tcccaaagtg tcggctgttt cgttatcgcc tcagattgcg cgaagtgggg
      781 ataagcttga agccatggtt gaagcgagcg atccagacca tgatcgggtc gacttgacct
      841 ataaatggta tcgtaatgaa tccgtgatta aggaaggaga agagcccttt ctcaatacga
      901 caggatttgt ggcacgggat aaaattacgg ttgaggtgac agcgcatgat cccgccgcta
      961 cagggaattc tttgaaatct gaaccgcttg ttttagagaa tagtgctccc aagattgtct
     1021 ccactcctcc tatctcagac gcgcaagacc gttttaacta cgctgtgaaa gctatggatc
     1081 tggatggcga ccagctaact tattatcttg aagccgcccc gaccggcatg agtatcagtg
     1141 agacatccgg tcatatccta tggcaggttc catccgatca aaagggaatc ttccatgtca
     1201 aagtagtggc caaagacggg catggtggta tggccacaca ggaattcgat ttgactttga
     1261 ccagcacagg tcccgctaag ccttcaggaa cctgaatcac ttccgatcgt tgtatccgct
     1321 tgtctcagct tttcttagtc tgttacaatc ctagcacgat gtaggaggag atcaccccaa
     1381 actcgtgcgt aagctcaaac tacgagaagg taccaatacg gccattctgt tcggtcacca
     1441 tgaccggcat ctcaagttga tcgaagacga gttgggtgtc cgactctctg cccgtgggga
     1501 agaactgacg ctggacggtc ttcccgaggc tgtccgtcag gcggaacgta ttctcgtcga
     1561 acttgcctcc ctaaccaacc aagggatatc actccaagct gaagatgtaa cccatgtgct
     1621 cagcgccttg cgccgagccc cggaggcttc gctcaaggat gtcctcggtc agtcggcgat
     1681 gatcgtcacg aaaaaacggt tcgtcggccc caagtcaccc acacagaagg cctacattga
     1741 cgcgatcgag cagcacgaca ttgtcatcgc catcggtccg gctggaacgg ggaagaccta
     1801 tctcgccatg gcaatggccg tcagcgcgct gataaacaaa gaggtaagtc ggattattct
     1861 tgcgcggccg gcggttgagg caggggagaa actcggtttc ctacccggcg atatatacga
     1921 gaaagtgaat ccttatctgc ggccgctcta tgatgcgctg tttgatatga tggacatgga
     1981 acgagcaaat cgcttgatcg agcggggaga tattgaaatt gcgcccctgg ctttcatgcg
     2041 aggaagaacg ctcaacgatt cgtttgttat cctggatgaa gcgcaaaacg ctacggcaga
     2101 gcagatgaag atgtttctca ctcggctggg gttcaattca aaagttgtcg ttaccggcga
     2161 catcacacaa gtcgatttac caagcgatcg agtgtccggt ctcattcaag tgaaagaaat
     2221 tctccgagac attgagggga ttgagttcgt gtacttcgat gaaaaggatg tggtccggca
     2281 tcggctggtt caagacatta tcaaggccta tgatcgtcat cagccggtaa atagcgacga
     2341 cccccggcat gctcgcgggc aagtcacctc tcaccagcgc ccatcaccca attccctcaa
     2401 gtcgcccgag gcccgctcgt cgccgcctgg tctaaccagc agttctcatt gatggctgtc
     2461 tatcttcgcg tgagcctcac gcggtttgct gtccgacaaa gcacactggc ccacctagcc
     2521 gaacatgtct tatcggtagt aggagagtct cgatcggaac taagcttgga attgactgga
     2581 gacagccgta tgcgccggct aaacctcgag taccgcaaaa atgattaccc tactgatgta
     2641 ctggcttttc ccatccgtga ggccgttatg cctcgaggcg tacgtcctct atccccgatg
     2701 cttggtgatg tcgtgatttc actaccgacc gccgttcgcc aagcaaggga ggccggacga
     2761 tcgatcgacg atgagctggc gatcctgttg gtgcacggcg tgcttcatct ttgcggatac
     2821 gatcacgaac gtagtgcgcg agaagcggcg cgaatgttgc gtcgtgaacg cgctctgctt
     2881 catctgatct ctcccgtgcc tcgcatggtc acgtttcgtg ctggacggaa accaagaggt
     2941 cgttgacgat gggatggttt caacggctga gcgaagggct cggcagaaca cgcaatgtcg
     3001 tgcagcagtc tctggatcga ttcctcggac gtgcaccgga tgaagaactt cttgaggatt
     3061 tggaggcggc gctgctcgct gctgacctag gcgcccgcgt cgtcgatcgt ttgatgctgc
     3121 aggtccgggg agagacgcgc ggagcggacg cgaacacttc ggaaggactt cagaatgtat
     3181 taagtcggtc gctctatagt attctgaaaa ccgtatcagg cccctcaatc gatcagctgg
     3241 tcaatgaaag ctctaaaccg cttgtcaccc ttgtcgtcgg ggtcaacggc gtaggaaaaa
     3301 cgaccaccat cgccaagatt gcacaacgga tgacgcaggc cgggcggcga ccgctccttg
     3361 tcgcaggaga cacctttcgt gcagccgcca ttgatcagct tcaagtttgg gggaatcgga
     3421 tcggagtgga ggtgattcat caacgacacg gtgcagatcc ggctgccgtg gccttcgatg
     3481 gtatcgttgc agccaaagcc aggacggtgg acatggttct aattgatacg gcaggccgct
     3541 tgcacacaaa gtccaatctg atggacgagc tgcggaaagt caaacgggtg atcgctcagg
     3601 agttgcccgg tgcgccacat gaagtgttgt tggtcttgga tgccacggtc ggacagaacg
     3661 cgctggccca ggcccgccaa tttcatgaag cagttggggt cactggtctt gctctgacga
     3721 agcttgatgg gactgcacgc gggggtatcg tcgtcgcgat tgccgaggaa ctgaagcttc
     3781 ccttgcgcct aatcggtgtg ggcgaaggag ccgatgacct ccaggacttc aatgccgagg
     3841 cgtttgtcgc ggctctgttc ggacagccga cttcccactc ataaaccacg gattgccttc
     3901 tcattattca cgctcgcgtg ttattctttt tcccaggtac agttttcagg ttgaggaaag
     3961 gtcggcagca atgatcaaag tcttgatcgt ggacgacgat cagatgaatt gcgatctatt
     4021 gcagagtgta tttacccgac aagggtgtca cgtcatttcg acgaccagtg gacgagaagg
     4081 tcttgatcta ttccgtgcac acaatccccg agttaccctc ctggatcttc gtatgcctga
     4141 gatggacgga ttgactgttt taaaggaaat ccgggctatc gatcctcatg cgccggtgat
     4201 tatcctgggc ggtggagcaa ccgaaattca ggagaatcaa gcacgcgctc tacgggccac
     4261 ggatttcatc agaaaaggat tgtcactgga cgtcctcgtc gaagccgtca atcaggtctc
     4321 aaaactacct gtaccgtcaa cgactgcgca gcccgctctt ggcaggggga atgtcattga
     4381 gcagatcgat gaatcggtct tggtggtcga tgacgaacct ctcctgtgcg acctcttggt
     4441 ccgttttctc agtctgcgcg ggtatcgggc gtttggtgtc aaagatggtc acgaggccct
     4501 gaggatagtc gaagatatac cgcccgatgc aatcgtgctc gaccttatca tgcccggaat
     4561 acaggggatc gaagttcttc gcaccttgcg tgacaagtgt tattcaggcg gtatcatcat
     4621 catgaccgga agtcacaatg aggagttgct tcgagaagct tggagtctag gccctcagga
     4681 gatccttata aaaccggtcg atcttgaacg cctcttgacc gcgatccagc ttgtactcgt
     4741 ctgccgcgag tgttaaaacg cttcctgatg tccatcagga aatatcttct tcgctcttct
     4801 ctcatcagtc tgctgtcgat ctccatggtg cacgcatggg gtgtttcggt ctcgtctgcc
     4861 caggagaatg ccgcaaacat tcagcacacc ctctcagccg agctgattct cgctgctcat
     4921 gagctggtgg caaacgatca gatcaaactg gaggttgagc cagaggtcac gtcggtcacg
     4981 ttcacgcttg cacctaccct gcatgtcgag tccatcgcca tgcaaacgca ctcggtctcg
     5041 ggcgggcaag gaaaccagga cgaagtcgtc ccattcacga gcgggtattc ttccgaggcc
     5101 acggttcagc acgtcgtcgt ctctcttcca aaagagcatg gacgcagggt gacgttggtc
     5161 atgagctatc gtggccagat caatgaccct ccgagagagc cgcgccactt gagatttgtg
     5221 acaccca
//
LOCUS       DATNDD010000465         3986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3830853, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000465 DATNDD010000000
VERSION     DATNDD010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3830853"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VN647_11255"
     CDS             <1..671
                     /locus_tag="VN647_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWG97652.1"
                     /translation="RMMVAQAREAGVPLMTAQTLRFDHTIQHMKMLQPSIGHSQQLDF
                     VFHIEIRKTVPDHADGYGKRGAVLEIGIHMLDLVRFLTGEEIQNVQCTMDRHPPSAPE
                     MRASAHLTTTGGTACRIDIARVLGSRAGRAIWIGSQGRVEADWMHPRIRCVDDAGVQT
                     MNADPPPSQTVLQTLTAFLQAVTNSSPMPITGEDGCRAVEIAEACYRSAQAGGKPITL
                     PLQL"
     gene            complement(692..928)
                     /locus_tag="VN647_11260"
     CDS             complement(692..928)
                     /locus_tag="VN647_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWG97653.1"
                     /translation="MATRAYILIKVKAGKTKDVVGTLKRIPGVEQAHSCFGRPDIFVF
                     ISVQDERALSDVVITKIHAIDGVEETDTHIVAES"
     gene            complement(1196..1636)
                     /locus_tag="VN647_11265"
     CDS             complement(1196..1636)
                     /locus_tag="VN647_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97654.1"
                     /translation="MAVLATLEQAQVLPPEGSREADRLIQSVIQFQSAFAKGTDRPVQ
                     DFAQRALTDKYGEEATPVLERFLASGWTAELLEALADADQRTSAEELERLATGFRQFN
                     LSVDDFKRFMQLVRDGRSALAARGQDFEGVFAHHRKAMPGAPGR"
     gene            1992..3521
                     /locus_tag="VN647_11270"
     CDS             1992..3521
                     /locus_tag="VN647_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWG97655.1"
                     /translation="MLAKVRSAALVGLDAHLVDVEVDISGGLPQFSVVGLPDAIVKES
                     RDRVRSALKNTGFHFPAKRITVNLAPAGIKKEGSGLDLAIAVGILVAEEVIPQSRLDH
                     RVLVGELSLDGRVKPITGALSFALACRAGYDLLLPADNGAEAAWVDGVSAYPLHSLPE
                     AVEFLKGTQAIVPSRSNHDVLGAAGTSEDEDYSDVRGQDHAKRALEVAASGGHNVLMV
                     GPPGSGKTMLARRLPSILPLLESEEAIETTRIHSVAGQLAPDRPLLAVRPFRAPHHSI
                     SDAGLVGGGTVPKPGEVSLAHNGVLFLDESPEFKRGVLEGLRQPLEDGQMILTRASGT
                     LKYPARFMLIAAMNPCPCGYYGDRTRPCICTGMQIRRYRAKLSGPLLDRLDIHLDVPP
                     VPVRELRTELPAAETSAAIRSRVVAARERQRRRYRHDGIYTNAQLKPRMVKRYCGLDQ
                     PAQELLEHAMVRLRLSARAHGRILRVARTIADLADSDKIEIVHIAEAIQYRSFDRNPD
                     M"
     gene            3529..3741
                     /locus_tag="VN647_11275"
     CDS             3529..3741
                     /locus_tag="VN647_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97656.1"
                     /translation="MASVKVFFWWFVVGATMALAVILVQGGIREVMQAQGSVWELKLV
                     ELLTTIIGGGLLGGCVALILDRIKKS"
     gene            complement(3806..>3986)
                     /locus_tag="VN647_11280"
     CDS             complement(3806..>3986)
                     /locus_tag="VN647_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97657.1"
                     /translation="LVQRQPVPRRFQFLEDDELPNSLVIARHRLLFQPGTTQNSEKMA
                     SSFVSQEGNKNEGIF"
BASE COUNT          802 a         1112 c         1197 g          875 t
ORIGIN      
        1 cccgcatgat ggtggctcaa gctcgcgaag ccggggtgcc cttgatgacg gcgcagaccc
       61 tccgattcga tcatacgatt cagcatatga agatgctcca accttctatc gggcattccc
      121 aacaactgga tttcgtgttt cacattgaga tacgaaaaac agttcccgac catgccgacg
      181 gctatggcaa acggggagcc gtgctggaaa tcggcatcca tatgctggat ttggttcgat
      241 tcctaaccgg ggaagaaata cagaatgtgc agtgcacgat ggaccggcat ccaccgagtg
      301 cgccggaaat gagggcctct gctcacctca ccacgaccgg cgggaccgcc tgccggatag
      361 acatcgcgcg agtcttggga tcacgtgccg ggagagcgat atggatcggt tcacaagggc
      421 gggtggaagc cgactggatg catcctcgaa ttcggtgtgt ggatgatgcc ggtgttcaga
      481 caatgaatgc cgatcctcct ccgtcccaga ccgtcctcca gaccctcacg gcctttttgc
      541 aggcggtgac aaacagttct cccatgccca tcacggggga ggacgggtgt cgcgccgtgg
      601 aaatcgccga agcctgttac cgttcagcgc aagcaggagg taagccgatt acgcttccgc
      661 ttcaacttta gcctcgacct gcagcgctcg gctaggactc tgccacaata tgggtatccg
      721 tctcttcgac accgtcgatt gcatggatct tcgtgatgac gacgtcggag agcgcccgtt
      781 catcctggac gctgatgaag acaaagatat ccggacggcc gaagcaggaa tgagcctgtt
      841 ccacgccggg tatgcgtttc agcgtgccga ctacgtcctt ggtctttccc gccttcactt
      901 tgatgagaat gtaggctctc gttgccacgt gtccctcctt ctagcaggat gctgaaaaag
      961 tccgccagcg gcgttctcgc atcgctcaga ggctcaacgt accgaagcgt acgcctcgcc
     1021 tcttcgctcg ctgcggtctt gctggacagc ctttttgagc atcctgcagt tattcttgcg
     1081 ttagcgccct acggaaaact cccagccatg ttgtttgcac cactgagttt ttcccagctt
     1141 tctaggtttc gtcgtttgtg aagcgtaaga tgtagcacat tcgcgagata catgattacc
     1201 gtcccggcgc tcccggcatc gcctttcgat ggtgagcaaa caccccttca aaatcttgcc
     1261 ctcgcgcggc gagcgccgac cgaccgtctc gcacgagctg catgaatcgc ttgaaatcat
     1321 cgaccgagag gttgaattgt cggaaaccgg ttgccagccg ctccaactcc tccgcggagg
     1381 tgcgctggtc cgcatccgcg agcgcttcca atagttctgc ggtccatccg cttgcgagaa
     1441 atcgttcgag aacgggggtg gcctcttcgc cgtatttgtc ggttagggcg cgctgcgcaa
     1501 aatcctgtac gggacggtcc gtgcccttcg cgaaggcgga ttgaaactga atgaccgact
     1561 gaatgaggcg atcggcttcc cgactgcctt ccggtggaag gacctgtgct tgttcgagcg
     1621 tggcgagaac tgccatagcc gatccgacat gcggagagga gcgctttggc tgctgcacgg
     1681 agaggggagg agtttggccc gcggtgagca aggaagaccc gctttccaag ggggaacata
     1741 gtaagagaga aaggctaatg gcgaaaaccc gacaagcctg ccggttggat cgtgacccac
     1801 tcaaagggat cgttccctct ggggaataca gaggcgcatg ggcggattat acagagtcga
     1861 tgggagcggt gctagagtgg acccggcggc ctgtcgctct attgaaaccc ccttagagac
     1921 ttcctataat cgccgggttt ggccgcggtc cagcccacat caataatgtg ggctagaaag
     1981 gcagtgttgt catgcttgcc aaggtgagga gcgcggcact cgtcggtctc gacgcgcatc
     2041 ttgtggatgt cgaggtcgat atctccggtg gtctccctca attctcggtc gtcggcttgc
     2101 ccgacgcgat cgtcaaggaa agtcgtgacc gagtgcgctc tgcgctgaag aatacgggat
     2161 ttcactttcc tgccaaacgc attaccgtca acctcgctcc cgccgggatc aagaaagagg
     2221 gatcgggact tgatcttgcg atcgccgtcg ggatcctcgt cgccgaagaa gtcatcccac
     2281 agtcgaggct ggaccatcgt gtgctggtcg gagaattgtc gttggacggt cgggtgaagc
     2341 cgatcacggg cgccttgtcc tttgcactcg cttgccgggc cggatacgac ctgttgttgc
     2401 cggccgacaa tggtgctgaa gcagcatggg tcgacggcgt cagtgcctat ccgctccata
     2461 gcctcccgga agcggtggag tttctgaagg gaacccaagc cattgtgccg agccggtcga
     2521 accacgatgt gctgggggca gccggaacat cggaggatga agactattcg gatgtccgtg
     2581 ggcaagacca tgccaagcgg gcgctggagg ttgccgcgtc cggtggacac aatgtgctga
     2641 tggtcggccc gcccggttcc ggcaagacga tgttggcgcg ccgattgccc tcgattcttc
     2701 cgctgctgga atcggaagaa gccattgaaa cgacgcgtat tcacagcgtc gccgggcaac
     2761 ttgctccgga tcggcccttg ctcgcggtgc ggccctttcg cgctcctcat cacagcatct
     2821 ccgatgccgg cctggtcgga ggaggcaccg ttcccaagcc tggagaagtc tcgcttgcgc
     2881 ataacggcgt gctgtttctg gacgagtctc ctgagttcaa acggggggtg ctggagggat
     2941 tgcggcaacc gttggaagat ggacagatga tcctgaccag agcaagcggc accttgaaat
     3001 atccggcgcg attcatgttg atcgccgcaa tgaatccttg cccttgcggc tattatggag
     3061 atcgaaccag accctgtatt tgtaccggca tgcagattcg tcggtatcgc gcgaaactct
     3121 ccgggccgct gctggatcgg ctggatatcc atctggatgt gccgcctgtt ccggtccgcg
     3181 agctccgtac tgaactgcct gctgctgaaa catctgctgc gatccgatcg cgtgtcgttg
     3241 cagctcggga gcgccaacga cgacgatatc gacatgacgg gatctatacg aatgcgcagt
     3301 tgaagccgcg aatggtaaag cggtattgtg ggctcgacca gccggctcag gagttgctcg
     3361 agcacgcgat ggtgaggctg cgcctatcgg cgcgggcaca tggacgcatt ctacgggtcg
     3421 cgcgcaccat cgccgatttg gccgactctg ataagattga gatcgtgcac attgcggaag
     3481 ccattcaata ccgttccttt gaccgcaacc ccgacatgtg aggattcagt ggcatcggta
     3541 aaagtgtttt tctggtggtt tgtcgtggga gcgacgatgg cgttggccgt catcctggtg
     3601 caagggggca tcagagaagt gatgcaggcg caagggtctg tgtgggaact gaaactggtc
     3661 gagctgctga cgactatcat cggtggaggg ttgttgggag gctgcgtcgc gttgatcctc
     3721 gatcgcatta aaaaatctta agtgtaagct ccctccaact ccgtaaagat tctccacgat
     3781 ccgtttcgtg tcctctgccc gcaaatcaaa aaataccttc attcttattg ccttcttgtg
     3841 atacgaagga agaggccatt ttctccgagt tctgcgtcgt tccgggttgg aagagtaacc
     3901 gatgccgcgc aatgaccagg ctgttcggca gctcatcatc ctcaagaaac tggaatcgtc
     3961 gcggcacggg ctgacgttgg accagc
//
LOCUS       DATNDD010000466         5726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3800314, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000466 DATNDD010000000
VERSION     DATNDD010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3800314"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VN647_11285"
     CDS             complement(<1..190)
                     /locus_tag="VN647_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97658.1"
                     /translation="MSDYKHRPIKLTPKQHGDTTWSCAYRIIDISSTGWRFHKGHSYG
                     SFGSREEAEKAALEEATQI"
     gene            complement(685..1251)
                     /locus_tag="VN647_11290"
     CDS             complement(685..1251)
                     /locus_tag="VN647_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97659.1"
                     /translation="MKKTCASLISAVMLLLAFPIALAVAETLEGLDTMWECPQADGTT
                     IYTNKERPGCEVMTLKPLSTVPDLATMPTIPRVSTDILRPHDSTPSPARPSPRSDRTV
                     PDWAKDWYASLAPSESVHEEVCSLYSEWMHLMLKTRGGFFFGSDPSYGADMTGRNQRG
                     PSYSYYDNTRYLALSKLFGRGFVPVGCF"
     gene            complement(1296..1577)
                     /locus_tag="VN647_11295"
     CDS             complement(1296..1577)
                     /locus_tag="VN647_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97660.1"
                     /translation="MMPVFPNTYRQYCHLLAKNQAYVITGVVEEQFSTVTITVRTLRL
                     LSTHPMEATIEPVEEVSVQPYPSGFPTPKWHRPPEHTLRRVRTQMGNDQ"
     gene            complement(1525..1788)
                     /locus_tag="VN647_11300"
     CDS             complement(1525..1788)
                     /locus_tag="VN647_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97661.1"
                     /translation="MAALASRANQKLASFFSQSPLCRPMHSGYQRGSPRDKEVTLIGW
                     LLTEKIVSTKKGEPMEFMTLEGQTGMYDASVSQYLPAVLPSAG"
     gene            2366..2728
                     /locus_tag="VN647_11305"
     CDS             2366..2728
                     /locus_tag="VN647_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWG97662.1"
                     /translation="MPTILVIDDAAPIRLLLSTALQSAGYNVVEAPSGREGLALYRQH
                     PSDVVIVDMLMPELNGLDTIMEFTREFMNVKVIAVSGMDSDQTMLQTAKLLGARQTLV
                     KPFDLPTLLHAVQYELQH"
     gene            2847..3164
                     /locus_tag="VN647_11310"
     CDS             2847..3164
                     /locus_tag="VN647_11310"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97663.1"
                     /translation="MAFIVRPYRRFPVVIPAIYENWSQEGQGMIWNLSSAGWRLSGDL
                     PLAPGDICSLTVMLPTNKSISVAAGVVRWVRGEEFGIETLAMNKTAHARLSTYIQERS
                     KAL"
     gene            3248..3511
                     /locus_tag="VN647_11315"
     CDS             3248..3511
                     /locus_tag="VN647_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97664.1"
                     /translation="MERKGLCTKQDLYDIIAELRKTRQQARIPETVFPSPYLLSQTEE
                     KVIEDILAVLNQHGMDAQQSLELLEQVGRIIEMGARMTQGTTH"
     gene            3523..3828
                     /locus_tag="VN647_11320"
     CDS             3523..3828
                     /locus_tag="VN647_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWG97665.1"
                     /translation="MTAIKRAIRFSLCALLLGAMPMFSLTVTTPTIYAAGPVDLNTAT
                     ADQLKGLSGIGESYTDKIIKNRPYARKDELVQKKVIPQATYDKIKDKVVARQKQQDR"
     gene            4356..5042
                     /locus_tag="VN647_11325"
     CDS             4356..5042
                     /locus_tag="VN647_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWG97666.1"
                     /translation="MPSRLRLSVLLLLLSLPSLVHSQDFSGQVVGITDGDSIHVMHND
                     RAEKVRLHGIDCPEKKQPFGRRAKQVTSALSFGQYVTVRRAGKDRYGRTLGTVELPNG
                     TNLNYELVRQGWCWWFRKYSPSDTELESLERAARQAKKGLWVDPRPVPPWEYRKAKRS
                     PKNPPPAASHSRASEKRISPAIIGNRDSHLYYRPHCPGYGQMAQEIRIEFASERDAEA
                     AGYHRAWNCP"
     gene            5204..5383
                     /locus_tag="VN647_11330"
     CDS             5204..5383
                     /locus_tag="VN647_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97667.1"
                     /translation="MDRHNELSVFLVDVQGQPLEALGHSMGDAGGATYYAKGGTYSLR
                     IASNAHWSVTVVQLP"
BASE COUNT         1353 a         1525 c         1551 g         1297 t
ORIGIN      
        1 cgatctgtgt cgcctcttcc agggctgcct tctctgcctc ttcgcgggat ccaaaactgc
       61 cgtaagaatg ccctttgtga aacctccagc cggtggagct gatgtcgata atgcgatacg
      121 cgcaactcca agtcgtatca ccatgctgct taggagttag cttaattggg cgatgcttat
      181 agtctgacat cggcatttct ttctgccccc gcaattccaa taacaaggct gaaaaagaac
      241 cctggtgaag gttttcctac tgtcctgagt cagatgtcac cctccggccc tccttcgttc
      301 tagatatgtg tctcgtcttg tggctgtagc cgagggcaat cttcttcagt cagaacgtct
      361 tcataattat ccgtttcctc gctcggggat atgtgtgagc ccttatcaaa cttcggcggg
      421 aggtcgtatt ctgcgcggag cttgttaagg aattgcgtaa attcttcgct gttcatcatt
      481 ttccgattcc tcctaccaga ctggtggtct tgataaagaa agggtaccgc ttcttacatc
      541 tctggctata ttcgaaagta ggtgacgcgg ctactcttcc gtaggctact gcgattgacg
      601 gccaccttta ttggagaacc gatcgaagcc gccgatcctc gccctgcagc gcattcttaa
      661 cgttgaaatc gcaacggctt tgtcttagaa gcatcccacg ggcacgaacc ctcttccgaa
      721 gagcttcgat agggcaagat agcgagtgtt gtcataatag gagtagcttg ggcctcgctg
      781 attacgccct gtcatgtctg cgccatacga aggatcagag ccgaagaaga acccgccacg
      841 agtcttcagc atcagatgca tccattcaga gtagagcgag cagacttctt cgtgaaccga
      901 ttccgatggc gcaaggctgg cgtaccagtc cttggcccag tcaggaacgg tacggtctga
      961 cctcggtgac ggacgggccg gggaaggggt gctatcatga ggacgcagga tgtcagtcga
     1021 gacacgagga atggtcggca tggtggctaa atcggggacg gtcgataggg gcttcagcgt
     1081 catcacctca cagccaggtc gttccttatt cgtgtagatc gtggtgccgt ctgcttgtgg
     1141 acattcccac atcgtatcta acccctccaa ggtctcggca acagctagcg cgatggggaa
     1201 ggccaacagc agcatcaccg ctgaaatgag cgaagcacat gtcttcttca taaaacacct
     1261 ttagaagcgg atggcaggaa agacctggga ccgttctatt ggtcgtttcc catttgtgtt
     1321 ctcactctac gcaacgtgtg ctcgggcggt ctatgccact taggggtggg aaacccagac
     1381 gggtatggct gcacgctgac ttcctcaacg ggttcaatag tggcttccat cgggtgcgtg
     1441 ctcaacagac gaagcgttcg gaccgtgatg gtgacggtgg aaaactgttc ctcaaccacg
     1501 cccgtgatca catatgcctg attcttagcc agcagatggc agtactgccg gtaggtattg
     1561 ggaaacactg gcatcataca tccccgtctg accctctaag gtcatgaact ccatgggctc
     1621 acctttcttg gtggagacga ttttctcggt cagcagccag ccgatgagcg tgacttcctt
     1681 gtcacgaggt gatccacgtt ggtagccgga atgcatgggt ctgcagagcg gcgattggct
     1741 gaagaaagaa gccagttttt ggttggcccg cgatgccaag gccgccatga aggaacggca
     1801 ttccgctcag ccatgtccca tgctgtacag aatgccttga ccactgttca attgtggagg
     1861 ctcgattggc taaaccattg acaggagtgc gctatcagaa ggatcacaat ttgctctcct
     1921 tctctgaagg tcgattcttc aggaggagga gttatggaat gccccaaatg ccatggcatg
     1981 ctgaagcgag agtggctttc agatttcttt tttagaatcc atacgtggaa ctgcaccaat
     2041 tgcggtgcca ttattgatcg aaccatctct tacaaccgac gcaaaagtct agcagcacgt
     2101 tccgccccag gtgaatctaa atctcagtca gctattgccc atgcgaaaac tattaggagt
     2161 aaagtctccc ggttggcttc atccctgtgc taacccgcta ggaatacact cactcatgcc
     2221 acaggaggga tatatgcagg cccaaaaata ccgaacgtcg gcgactcaca ttaaaaaaca
     2281 taaatggaag agtcctcagc agttgagctc gaagtccaga cctacacaga taggctccga
     2341 ttcacctaga tatggaaggc agtatatgcc cactatattg gtaattgatg atgcggcccc
     2401 cattcgcctg ttgctttcta cggcgcttca atcggcagga tacaacgtgg tggaggcgcc
     2461 ctccgggcgc gagggcctag cattgtaccg tcagcacccc tcggatgtag ttattgtaga
     2521 tatgctgatg cccgagttga atgggcttga taccatcatg gagttcactc gtgaatttat
     2581 gaacgtcaaa gttattgctg tctccggcat ggacagtgat cagaccatgc tacagacagc
     2641 caagctcttg ggggcgcgac agaccttggt gaaaccgttt gatctcccta cgttgcttca
     2701 cgcggtgcaa tatgaattgc agcactagcg gcgaaggaga gtcaggcgtt gcatgacgta
     2761 cccgccacat tctcgatcct cctcccaaag gtctattgca gatcacgcct ccagcctcct
     2821 agaatttggc ctcgcgtggg gtctctatgg cgtttatcgt gcgtccttac cgtcgcttcc
     2881 ctgtcgtcat cccggcgatc tatgaaaact ggtctcaaga ggggcagggt atgatctgga
     2941 atctctcatc ggctggctgg cgtctctcgg gggatctccc gttagcgcct ggtgacattt
     3001 gctctctgac cgtgatgctt cccacgaaca aatcaatctc ggttgcggcg ggggtggtgc
     3061 gatgggtacg aggcgaagaa ttcggaatag agacactggc gatgaacaag acggcgcacg
     3121 cgcgtctcag tacctacatt caggagcgca gcaaggcgtt atgaatgacc ggcctaaacg
     3181 ggaaagcatg tccctggagg aagccacgat tttcaacatg taggagattg cggcgctcgt
     3241 cgaagtcttg gagcggaaag gtctctgcac caaacaggat ctgtatgaca tcatcgccga
     3301 gttacggaag acacgccaac aggcccgcat tcctgagact gtgtttccgt ctccgtacct
     3361 cttaagtcag actgaagaaa aagtaataga ggatattcta gccgtcctga accagcacgg
     3421 gatggacgcc caacagtctc tggaattact agaacaggtt gggcgcatta ttgagatggg
     3481 ggcaagaatg acccagggga cgacacattg aaagggggca gaatgacagc cataaagaga
     3541 gccattcgtt tcagtctgtg tgcgctgcta ctcggggcga tgcccatgtt ttccttgacc
     3601 gtgacgaccc ccaccattta cgccgctgga cccgtggatc tgaacactgc cacggcagac
     3661 caactcaaag ggctctcggg gattggggag tcctataccg acaagatcat caaaaatcgc
     3721 ccgtatgcgc gaaaagatga gttggttcaa aagaaagtca tcccacaagc gacctatgac
     3781 aagatcaaag acaaggtagt agcgaggcag aagcaacaag atcgttgaca ggagtgtgcc
     3841 ccccttcatt ctaggacagc accagccaga attgtagatt ctctggtcat gcgtgacccc
     3901 gtcaggcagc ataacgggat gctccgtcca ggactccctc gaacgtcacg gcctacgact
     3961 acgcagccag ttggcgttgg gttactaggt gagtatcgag aggagttatt cgcccttgga
     4021 gtcaccccgg cccacgcccg aatcttgctt tatctccaac gacatcccaa tagttacatt
     4081 ctgcagtgcg caagggcatt tggtctgacc aatcgaacgg tcgggtatcc tgtccaggtg
     4141 ttacagcgaa ggcactgggt aagaaaacga cgagcgcccc aggatgaccg gtatgtattg
     4201 ctgacgctga ctcagcgagg ccaagctctg gcacggaaaa tccacaagcg gcttaatgaa
     4261 cggactctgt taaggcggcg tcatgacaca cgtgacagct caatgagagc cttttactgg
     4321 cgtcactatt gccgaccggc tagaatacat gactgatgcc gtcacgcctg cgattgtctg
     4381 ttctcctgct gctattgagt ctcccgtcac ttgtccattc tcaagacttc tccggccaag
     4441 tagtcggcat cactgacggt gattctatcc acgtcatgca caacgatcgt gctgagaaag
     4501 tccgcctgca cggcatcgac tgccccgaga agaagcaacc cttcggccga cgagcgaagc
     4561 aagtcacttc ggccttaagc tttggccagt acgtcaccgt ccgccgggcg ggcaaggatc
     4621 gatatgggcg cacccttggg acggtggaac tcccaaacgg gacgaacctg aactatgaat
     4681 tggttcgtca gggttggtgc tggtggttta ggaagtactc tccgtccgac actgagctag
     4741 aaagcctcga acgggccgca cggcaagcca agaagggcct ctgggttgat ccccggcccg
     4801 tccccccatg ggagtatcga aaggccaagc gaagcccaaa gaatcctcct cccgctgctt
     4861 ctcattcaag ggcttcagaa aagcggatct ctcctgccat tatcggcaat cgagacagcc
     4921 acctctacta ccgaccccac tgcccaggct atgggcagat ggctcaagag atccgcatag
     4981 agtttgcgag tgagagggat gccgaagcag cggggtatca tcgagcgtgg aattgtccct
     5041 gacaggaggc agatatgtat caactcatcg gtgtgctact ctgcgggtgg ctcatgttca
     5101 cagatgtggt actcgccgag gaagaacaga ttgtgatgga actgactgga aatggttcgc
     5161 ggacgactcg accattcacc gtaaaagatg gatgggaagt tcgatggacc gccacaatga
     5221 actctcggtc tttcttgtcg atgttcaagg gcaaccactt gaagcattgg ggcattcgat
     5281 gggcgatgcg ggtggagcaa cgtattacgc gaaaggcggg acctactcgc tcaggatcgc
     5341 aagcaatgcc cattggtctg tcacggtagt tcagcttccc taacatgctg caacacgcct
     5401 caaaccacta ggccacatag ggcacgacaa acgcaggggg gtagcgtggg ttatggggta
     5461 atcctgagcc tataccgcca tgggagtgtc ggaagggaat tcgatgaggt ggcggactcg
     5521 gggcatgcag atcttctcac cgaacgttgg gcagcccgtc atagggtcac attttgcggg
     5581 cagacagggc atggaatcac gtcgcgctcc aactcaagcg tggcacagca acaggtatcc
     5641 tcgaagcaat cggcagtgat aaacccttta ccgctacaac ggtaacaacc gtcggccatt
     5701 tcatcaccgt catccataac acctcc
//
LOCUS       DATNDD010000467         5258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1921440, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000467 DATNDD010000000
VERSION     DATNDD010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921440"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VN647_11335"
     CDS             <1..1336
                     /locus_tag="VN647_11335"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWG97668.1"
                     /translation="PKRCSFYAGVRRELIPIEEHCGKIRAAKAAQVFPEFMVIARTEA
                     LIAGWGQEEALKRAEAYAGAGADAILIHSKSKKFDELKTVYKLWSGRVPLVVVPTIFD
                     QTTAAEMEEAGAKIIIYANQPVRAAIRAMTDTLGLIKQDTRPGAANDRIVTLPEVYDI
                     VGVPQMEHDEKQFLPVGSERITAIIAAAGFEKQLLPLIEDKPKCLLDIKGKTILDRQI
                     SALNECNIKEVALVRGYKKEAISLPNIRYYDNDRYEETGELVSLFCAENELRGRTVVL
                     YGDIIFDTAILEKLLKSPADIALVVDLAWFDQEQRNAQPTHLNPDLVSLASPPGKSYL
                     SRFVMPEGEHQVVKIGQQLPGELAHGEFIGMAMFSEKGTQALRESYHMAQKQLQSSPF
                     HESASLAKASFTDMLQELIDQGYRIQAVPIFKGWMEVDSFEEYQKAWAKIRQ"
     gene            2237..2620
                     /locus_tag="VN647_11340"
     CDS             2237..2620
                     /locus_tag="VN647_11340"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97669.1"
                     /translation="MKPVKHLRQPETPYSSHSIERRRMRRTCASFGLMYSGINGDDVF
                     IGDGSAVDLSESGLGIRGNCPVNVGMELTLFLYLLDDEEPLFVSEATVAWTAGSLFGV
                     EVKEVSLHDADRMLSFLRAQSVGQA"
     gene            complement(2993..3331)
                     /locus_tag="VN647_11345"
     CDS             complement(2993..3331)
                     /locus_tag="VN647_11345"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWG97670.1"
                     /translation="MKTTTVKEVLDSLPSKLDKDAAEDLDAVYQFDLRGNQGGQYHLL
                     VQNGTCVVRDGTHADPHVTLSMTGEDCIGVLNGQFSGMTMAMSGRLQVTGDIGLAMQL
                     KSLFPNVVES"
     gene            complement(3427..3867)
                     /locus_tag="VN647_11350"
     CDS             complement(3427..3867)
                     /locus_tag="VN647_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HWG97671.1"
                     /translation="MSTSASSESAILPPNAPAAAVQPVQDMVGSGKAWGLCTAVDLYD
                     CNPDLIRDANYIKRYVVELCALIGMKRYGECQVVNFGEGRVAGYSMVQLISTSLISGH
                     FANETNHAYLDIFSCKGYDPTVVESFSKEFFGARRSVATATLRY"
     gene            complement(4244..4948)
                     /locus_tag="VN647_11355"
     CDS             complement(4244..4948)
                     /locus_tag="VN647_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWG97672.1"
                     /translation="MEFVQAKVSRIRNLTHDVRELELILCEPEGISFKAGQFISFDIS
                     KEGYPYPVTRPYSIASPSSTSDRVLLLLNLVPGGPGSTYLFSLREGDPVRFKGPAGSF
                     TLREDPTKRMLFVATGTGIAPFRSMILSHLEGDGTRPLMLFWGVRSERDLYYQDEFQD
                     LADRYPHFSFVMTLSQPGPEWTGGRGRVSSLVQDRVTSVRDLAVYLCGNGNMIKDVTA
                     YIQAKGLCPIYREKYY"
BASE COUNT         1334 a         1380 c         1303 g         1241 t
ORIGIN      
        1 tccaaagcgc tgcagtttct acgccggcgt tcgacgggaa ttgattccca ttgaagagca
       61 ctgcggtaag attcgagcag cgaaagcggc tcaggtcttc ccagaattca tggttatcgc
      121 cagaaccgag gccctcattg ccggttgggg acaggaagaa gcgctgaagc gagctgaagc
      181 ctatgcaggc gcgggcgccg atgcgattct catccattcg aaatcgaaaa aatttgacga
      241 attgaagacc gtgtacaaat tgtggtccgg acgcgtcccg ttggtcgtgg ttcccacgat
      301 tttcgaccag accactgcgg cagaaatgga agaagcaggg gcaaagatca ttatctatgc
      361 caaccaaccg gtacgggcgg ccattcgtgc gatgacagac acgcttggcc tcatcaagca
      421 ggacacgcgc cctggagcgg cgaacgaccg aattgtcacg ctacccgaag tgtatgacat
      481 tgtcggtgtc ccgcaaatgg agcacgacga gaaacagttt ttgccggtgg gaagtgaacg
      541 aatcaccgca attatcgctg ctgccggatt cgaaaaacag ctcctgccgt tgatcgagga
      601 taagccgaag tgtctgctcg acattaaagg gaaaactatc ctcgatcgcc agatcagcgc
      661 actcaacgaa tgcaacatca aagaagttgc gttggttcgc gggtataaaa aagaagcgat
      721 ctcgctcccc aatatccgct actacgacaa cgatcgctac gaggaaaccg gcgaactcgt
      781 ttcgctcttc tgtgccgaaa atgagttaag ggggcggacc gtcgttttgt acggcgatat
      841 catcttcgat accgcgatcc ttgaaaagtt gctcaagagt cctgccgaca ttgctctggt
      901 agtcgacctg gcttggtttg atcaggaaca gcggaatgct cagccgactc atctcaatcc
      961 ggatcttgtc tcactggcct cccctcccgg aaaaagctat ttgtctcgat ttgtgatgcc
     1021 cgaaggtgag caccaagtgg tgaagatcgg gcaacaactt ccaggtgaac tggcgcatgg
     1081 tgaattcatc ggcatggcca tgttctcaga aaaaggcacc caggcccttc gcgagtccta
     1141 ccacatggcc caaaaacagc ttcagtcctc cccgttccac gagtcagcaa gccttgccaa
     1201 ggcatctttc accgatatgc tgcaagaact gattgaccag ggctatcgca tccaagccgt
     1261 accgattttt aagggatgga tggaggtcga ttccttcgaa gagtaccaaa aagcctgggc
     1321 aaagattcga cagtaattcc ctggatctta cggtagcggc ttctctcacc accggaaata
     1381 ttctttaaag gtagtgtatc acctcgcttc tcattagcag gctcctttgc tgaattgatt
     1441 aggacaatat tcccaacgaa gctatgatgg tgaaactgac gatgagtggc cgaaaatctt
     1501 acgtgccttt agataatcgt tatgccgaac gtgtagccgt cacgtgtcga gtgcgctatg
     1561 tcggtgaagt tccgacgcag ccacaccaag gcgaaggact cacaaaaaac atatctgttt
     1621 ctggttgcca cgtcatcagc gaccgcccgg taacacgtgg aacactattg actcttacca
     1681 ttctgctcac cgatggatta ccccaactgt tattgaaatc ggcgcatgtt gtgtgggtgt
     1741 cgggttgtca attttctgtt cgatttatgg atctgaaccg agaccatcga aagcgcctgc
     1801 aatctttcat ttggaaaagc atttctcacc acaccgtgag cgatcaacga actcgattcc
     1861 ggcttatgta gccacccagt cctatactcc cggcgattct tttcataaaa gaacttacct
     1921 cctcatcaac ttctaagatc tcgcatactg cattctgaag caggtatcct ctataacaat
     1981 gaattttgcg tcatccttcg ccccccttcg gaactgagca tcacccccca tctagtggga
     2041 aggcaaggtt gacgctccgc gataaccttc atcaatacaa aatctccaaa taaagcagac
     2101 acgattatag gctggcgaca cgagaggcaa ggatcatatg aacagatgga gatccgtatt
     2161 cagcccagca gaaaggaccg atgagcccaa aaaggagcgc cattcgaaaa cagatataca
     2221 gccaggaggt agaacgatga aaccggtgaa gcacttgaga cagccagaaa caccatactc
     2281 atctcattcg attgaacgtc gcaggatgag aagaacttgt gcctcctttg gccttatgta
     2341 ttcaggaatc aatggggatg acgttttcat tggagacggt agcgctgtcg atctatccga
     2401 aagcggatta ggcattcgtg gaaactgtcc ggtcaatgtt ggcatggagt tgaccttgtt
     2461 tttatatctc ctggacgatg aggagcctct gtttgtttcg gaagcgacgg tcgcctggac
     2521 ggcgggatct ctgtttggtg tggaagtgaa ggaggtgagc ctccatgatg ccgatcggat
     2581 gctgtcgttt ctccgtgctc aatcagttgg tcaagcctag tcggttcgat ggcccgacaa
     2641 ttgccgcatg catgattggg aaggctaaag gctagtagtt gctaagagcc atatcgccct
     2701 agacaccaca aacttcagaa gggctccacc tacttctatc gtcgctatac tctcaaccgt
     2761 tcgacttcaa taagcgattc caccttgtcc tcatacacgt attatgttaa ttcattggtg
     2821 agtgaatact tactcaccat gaatattgag cgtctctaca ctcctcacgt tgttcctcgt
     2881 cccaacgatg tacgcgattt tagaagaacg gttcccgaga agacttgatt tggagatgcg
     2941 agatcaccgg aaagctcagg ccggaggggt ttctgttccg gagtaatgct cgttaactct
     3001 caacaacgtt ggggaacaag gatttcagct gcatagccag cccgatatcc ccggtgactt
     3061 gcaaacgccc cgacatcgcc attgtcatcc cgctgaactg tccatttagg accccgatgc
     3121 agtcttctcc ggtcatcgag agggtcacat gcggatcggc atgggttcca tccctcacga
     3181 cgcaggtccc attttgcacc agcaggtggt actggccgcc ttggtttcct cggaggtcga
     3241 actggtagac ggcatcaaga tcctcagccg catctttatc gagttttgac ggaagtgagt
     3301 cgagcacttc tttgacggtc gtggttttca tgtcttggct atatctatgg cattggatgg
     3361 ttaaagtatt cgggggcgct ccactaacct agcgccccca aagcaagatc cctgtgaaca
     3421 aaagttttag taccgaagtg tggcggtcgc aacactacga cgggcgccga aaaactcctt
     3481 cgaaaacgac tcgacgacgg tgggatcata ccccttgcag ctgaaaatgt cgaggtaggc
     3541 gtgattggtc tcattggcaa aatggccgct gatcaatgag gttgagatca gttgcaccat
     3601 cgagtagccg gctacacggc cttcgccgaa attgacgacc tggcattcac cgtagcgctt
     3661 catgccgatg agcgcacaga gctcaaccac atagcgcttg atataattcg catcacgtat
     3721 aagatcggga ttgcaatcgt agagatcaac agcggtgcag agcccccatg ccttgccgct
     3781 tcccaccatg tcttggacag gttggacagc agccgcaggg gcgttgggtg gtaagatcgc
     3841 ggactcggaa ctggctgagg tactcataga aggttgatcc ctttctgtaa aaggttgtgt
     3901 ggacaagaac gatcatgcgt gaactatacc ataaattctc ttgctatgaa taagaaccgt
     3961 gaggaatatt tcggcttttg aaaagggacc cgtcatcacc aattgattga caaggtcacg
     4021 gagctcagga acaatatacc tcatgccatc cgcctctaac acatcccgga tcgagttacc
     4081 tgatcgcctc tgcacttggt gcaaagtcct catgaggaag cgcctggtgg gcggcaacca
     4141 gttcatccat tacacctgcc ccaaatgcat ctttcagcac accacccggc tcggattcaa
     4201 gcttcagaaa ccagctccat gattctggcc caaaccctcc ggtctaatag tacttctccc
     4261 ggtaaatcgg acataacccc ttcgcttgaa tgtacgccgt cacgtccttg atcatatttc
     4321 cattaccgca taaatagaca gctaaatccc gaaccgatgt cactcgatcc tggacgagac
     4381 tcgatactcg ccctctacct ccggtccact ccggccctgg ctgcgagagg gtcatcacaa
     4441 aggaaaaatg cggatagcga tcggccaaat cttggaactc gtcttggtaa tacaggtctc
     4501 gctcgcttct gactccccag aacagcatca gaggtcgtgt gccatcccct tcaaggtgcg
     4561 agagaatcat ggatcgaaag ggagcaattc ccgtccctgt cgccacaaac agcatccgtt
     4621 tggtcggatc ctctcgcagt gtgaaggatc cggctggacc cttaaagcgt acgggatctc
     4681 cttcccgaag actgaaaagg taggtcgatc caggtcctcc cggaaccaga ttgaggagca
     4741 agagcacccg atcggaggtt gaggaaggtg aagcaatgga ataaggacgc gtgaccggat
     4801 agggataacc ttcctttgat atgtcgaatg agataaattg cccggcttta aagcttatgc
     4861 cctcgggctc acaaaggatg agctctagtt cgcggacatc gtgggttaaa ttcctgatcc
     4921 gactcacctt agcctgaaca aattccattg acggctgatt cctctattct gaccaccgat
     4981 agctccatct ctttacggta acacatatca tctctggcca ttggcgaaga gtgcgaagcc
     5041 tactgccacc tggaagaacg ggcttagctt atctcccaat cccacttttc aaaggagaaa
     5101 ctgcaggcga ggacggtctt ccatgcgttg atgcgcctaa ccgcgggaaa acctactctg
     5161 gacatgatcg tcgagaatat gaatcaatcg ctctcgattt tccggaccga ttgaaacaat
     5221 ttttgcgcct aatttaggtg aacgcacgtg tatgactt
//
LOCUS       DATNDD010000468         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1925612, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000468 DATNDD010000000
VERSION     DATNDD010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925612"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VN647_11360"
     CDS             <1..1164
                     /locus_tag="VN647_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWG97673.1"
                     /translation="LPEGTIGILGEGEDAILKVIEGQSIEDERYIVKEGNRIRKGRQG
                     SPALLDALTVDLPYLTSIFPQYGEYIGESIGVQSKRGCPYDCAFCLYPYIEGKRVRYR
                     PPDMVVKDIAQHYHQWGARRFWFTDAQFITGKEAYPQCTEILERIVAEKLEIEWSGYI
                     RTSLITPDLAKLMVRSGVGDLEVAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFK
                     GKVILNYSLNSPKETEESLLQSVEAYKKVASILGEERVFPLMFFLGIQPNTDLEQRLL
                     EEGYLSAGYNPLMLTPSSIKKLLYNPAPLNKFIAKACLRAWERKQGSRDPRPWTGSLS
                     QTSESPTYADQNLSRGIEGNSGRDALLSLEEILRSRRPVPSPSQTPEPRTSAG"
     gene            1367..>2627
                     /locus_tag="VN647_11365"
     CDS             1367..>2627
                     /locus_tag="VN647_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWG97674.1"
                     /translation="MYKTIYIPVDNSDHSNTAVDVGVQLAKTFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPV
                     ERRSLEGRNWKVLAEDINTNGYDLVIMGALGVGAVKDSVIGSNTERVIRRVRNSDMLI
                     IKNVQPITGGRIVVAVDGSPYSFGGLMTGLALGKAFNMPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEIIDGGLAKIYQSHLDISREIAQAEQTDVKTTLLDG
                     KAFEKIIQYVRKDVPALLIVGRIGVHSDDDMDIGSNTENLLRSAPCNVLVSNRKYVPP
                     IDTQAEYTIAWTEEALRRMEKIPVFARGVAKTAIHRYAIEKGHTIISNTVVDAAVGHI
                     LPKGAMEAMRALGGNLDAAGIDRDKMQA"
BASE COUNT          647 a          731 c          687 g          562 t
ORIGIN      
        1 ctcccggaag gaaccatcgg catcctcggt gaaggagaag acgccatact caaagttatc
       61 gaaggccagt ccattgaaga tgaacggtac atcgtcaagg aagggaaccg gattcggaaa
      121 ggccgacagg gttcgccggc gctgctcgac gcgctgaccg tcgatttacc ctacctcacg
      181 tcgatctttc ctcagtatgg tgaatatatc ggcgaatcca tcggggtaca gtcaaaacga
      241 ggctgcccct acgactgcgc cttctgcctc tatccctata ttgaaggcaa gcgagtccgc
      301 tatcggcccc ctgacatggt cgtcaaggac attgcccaac attatcacca gtggggtgcc
      361 cgtcgattct ggtttacgga cgcccaattc atcacaggga aggaagccta tcctcaatgc
      421 acggagatcc tcgagcggat cgttgccgag aaacttgaga tcgagtggtc cggctacatc
      481 agaacctcgc tgattacccc ggatctcgcg aagctgatgg tccggtcagg cgtgggcgat
      541 ctcgaagtag ccattacgtc gggctcgcaa gaggtgctga acaatctgca tatgggattc
      601 aagctggaac gcctctatga cggctgtcgt tatctggccg aggcaggctt taagggcaag
      661 gtgatcctga actactccct caatagtcca aaagaaacgg aagagagcct tctccagagc
      721 gtggaggcct acaagaaggt cgcctcaatt cttggagaag aacgtgtatt ccccttgatg
      781 ttcttcctcg gtattcagcc gaacactgat ttggaacagc gactgttgga ggagggctac
      841 ttgtcagccg ggtataaccc attgatgttg actccgagta gtatcaaaaa gttgctctat
      901 aatcctgccc ccctcaacaa gttcatcgcg aaggcttgtc tccgagcctg ggaacggaag
      961 caaggaagcc gtgatcctcg tccatggacc ggatctctgt cccaaacttc agaatccccc
     1021 acctatgccg accagaacct cagccggggt attgagggta attcggggcg ggacgcactt
     1081 ctctcacttg aagaaatcct tcgctcgcgc cgaccagttc catccccttc acagacacct
     1141 gagccaagaa cttcggccgg gtaatttctc ttcagaatgc gtccgaatca ccatattgaa
     1201 cccatatctc tcgatctccc gctttcgtga cgccttgcat tccgatttgg gccagtgcta
     1261 tcatttattt tcttttgaga agtgggacag cacgtcaacc atttggtttt tcagataatt
     1321 acagtcccct tcatctacct aactcgcgcc ttaaggaggc gcctcaatgt ataagaccat
     1381 ctatattcca gtcgataact ctgaccattc caatactgcg gtggatgtcg gcgtccagct
     1441 ggccaaaacc ttcggctcga aaattgtggg aagccatgtc tatgccgcga aaatgcatga
     1501 caagcgcttc aagcagatgg aagccggatt gccggaggaa taccacgatg aaaaggagct
     1561 cgaccggcaa cgacagatcc atgactcgtt gatcacccgc ggtctccaaa tcatcaccga
     1621 ctcttacctc gactatgtcg acaagaaatg caacgaagcc aatcttcccg tcgaacggcg
     1681 atcgctcgag ggccggaact ggaaagtact ggccgaggac atcaatacca acgggtatga
     1741 tctcgtcatc atgggagcat tgggagtcgg tgcggtcaag gacagcgtga tcggcagtaa
     1801 caccgagcgc gtgatccgcc gagtccgtaa ctccgatatg ctgattatca agaatgttca
     1861 gccgataacc ggagggagga ttgtcgttgc cgtcgacggc agcccctact cgttcggcgg
     1921 actgatgacc ggcttggccc tcggcaaggc cttcaacatg ccggtggaag cgatttcggc
     1981 gtttgaccct tattttcact atgccgcgtt ccatagcatt tccggggtct tgaacgaaga
     2041 agccggcaag gtgttccgtt tcaaggaaca ggaaaagcta cacgaagaaa tcatcgacgg
     2101 cggtctggcc aagatctatc aatcccactt ggacatttcc cgtgaaatcg cccaagccga
     2161 gcaaaccgac gtgaaaacga ccttgctcga cggcaaagcg ttcgaaaaaa tcattcagta
     2221 tgtccgcaaa gacgtcccgg ccttgctcat cgtcggacgc atcggcgttc atagtgatga
     2281 cgatatggat atcggcagca acacggaaaa cctgctccgg agcgcgccct gtaacgtact
     2341 ggtctccaat cgcaagtatg tcccaccgat cgatacccaa gctgagtaca cgatcgcctg
     2401 gacagaagaa gcgctgcggc ggatggaaaa gatcccggtc tttgcgcggg gggtcgccaa
     2461 aacggctatc catcggtatg ccattgaaaa gggccatacg atcatcagta acaccgtcgt
     2521 ggacgccgca gttggacata tcctacccaa aggcgccatg gaagccatgc gcgcactcgg
     2581 tgggaatctg gatgcggccg gtatcgaccg cgacaagatg caggccg
//
LOCUS       DATNDD010000469         4822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_373014, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000469 DATNDD010000000
VERSION     DATNDD010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373014"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3069)
                     /locus_tag="VN647_11370"
     CDS             complement(<1..3069)
                     /locus_tag="VN647_11370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWG97675.1"
                     /translation="MRSNKKLSLKSDKSQTTRGHKRGVVGYSRKTALLEQAITQMNAG
                     KYGRSSAALKELLALDPHNMEARRLFATLHLRLGSLIPARQAFDSLISEAFQRQDYWL
                     AESLLREYLAAGPRCVPFLEKLGALYQEKGNALEAVGEYGKAIDILIEDPEPDNPHHA
                     AQLYAKIRELAPASPVAFRLASSFDTQTGELLTRQSGDSGQSTIAPSPDLSEGDQSIQ
                     AGSKPMDGVMPWEIQDFQATAPETFKVADVSDSDVSDSSGSLIPPTITDLPAPQGPTR
                     DGDWVPSDPPLREQTADDASEVRNDVPATESSEAQHGPDPAVPTGGPAAVQEQDRLSA
                     EAEPEAPYDLDVSGSPTVSTPIESADASEPPTSSAQSIEAAGVTPSSQIEEISASTYL
                     DTEPPTAGPIEVEDAQPSIHKSVETASKEVITEPPPDVRAPSSPESVSANEISEPWKQ
                     PGFSWESVFNSAWKFGSDHSANPSLPVVTQTDVDEVHTPASAEPPLRELALESRAVEI
                     PEREAPTSLGEQTPSGFPIAPMPWDQIQESVISILPSQTDQPAAERAGSTVDRSAGEV
                     ESLSEVSDDQAQPPIFRVSPAAEMETFSIAPALQTPVSPEPEIPAADVKPVSIQTESD
                     FTFVKPEQPPPASSLTMDEPPALVVPIAAIASPIFESESLTDDRVVPTSETLSKETED
                     IPISPPLPERQRIRELPPEIATTPHLAEEPTEAQTHERQPQASASVEKRTPIPESIPE
                     PVPEQEEWGKVGESIRFITPQTSPIAHVPPSISERQEVSRSMSVAAAAVDVLFESSRN
                     VKAPETRGSIAEPKPHRESSTTLSRIRVAMVGFVGSCFSTTHAIVATCVGLVMLLGIF
                     TAFAIGAIGLTWIIMEESPSPAFQSFTTTPQRTLSDFKKNGYFLLLGIDASVGRDPIQ
                     EGYDRKPETNDGNSASACFGGSGSRTIKRSNASADVMHGWVRSSDPVGEFKMHQETIK
                     GWGNQHQLALNRYGRWQKLPFEDWGYGQPVGPPCTAMIFAHQLHVADGFLQ"
     gene            3781..>4822
                     /locus_tag="VN647_11375"
     CDS             3781..>4822
                     /locus_tag="VN647_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWG97676.1"
                     /translation="MALDFNEVEIPVFVRFISELTGKNFVLDETIKKQGGKISVFSPT
                     KVLPDQAFNMFVAALEAARLAVVPKGGNLYQVVPMGDLPPERGVFVYKLKHANATDLA
                     AVLTNLVARSQTVAQITPGTRPPIRPLTEFEAPVQVFADKATNSIVISSTKIAWNHLQ
                     GVIRDLDIRRKQVFVEAVILEVEVDRLRQIGTDPTQVLGAGKSGFVQGIGGFNRAPED
                     LATVAQAISGVAAGGATGGAATVLNTLNVRAFMNLLLSLTDTNILSTPQVLAADNQKA
                     KIVVGENRPFPTGQAQGITGGTLVTIERKDVGVTLELTPQVLEDDLIRLEIKQEITAI
                     ETNEAQTIGSGSA"
BASE COUNT         1056 a         1308 c         1318 g         1140 t
ORIGIN      
        1 ttgcaggaac ccatccgcca catggagttg gtgagcaaag atcattgcag tacaaggtgg
       61 gccgacaggt tggccgtaac cccagtcttc aaagggaagc ttttgccacc ggccgtatcg
      121 attgagcgca agctgatgct gattgcccca tcctttaatg gtctcctgat gcatcttgaa
      181 ctcaccgacg ggatctgaac tgcgaaccca gccatgcatg acatcggctg atgcattcga
      241 ccgcttgatc gtcctcgatc ccgaacctcc aaaacaagcc gacgccgaat tgccgtcgtt
      301 ggtttcaggt ttccgatcat atccttcctg tatcggatcg cgaccgaccg acgcgtcaat
      361 cccgaggaga agaaagtaac cgtttttctt gaaatccgat agggtgcgtt gaggagtagt
      421 ggtgaagctt tgaaatgccg gagacggcga ctcttccatg atgatccagg ttagtccgat
      481 ggctccgatc gcgaacgcgg tgaagatccc caggagcatc accagcccca cacacgtcgc
      541 cacaatagcg tgcgtggtcg aaaagcatga cccgacaaag ccgaccatcg caacgcggat
      601 tcgactcagc gtagtactgg attctcggtg tggctttggc tcggctatag atccgcgagt
      661 ctcaggcgcc ttgacgtttc gggacgactc gaagagcaca tctacagcag cggcagccac
      721 cgacatcgac cggctgactt cttgccgttc ggaaatcgac ggaggcacgt gggcaatggg
      781 agaggtttga ggtgtgataa atcgaatgga ttctcctact tttccccatt cttcttgttc
      841 gggcactggt tcaggaatcg attcaggaat aggagttcgt ttctcaacag acgccgatgc
      901 ttgcggctgc cgttcatgag tctgcgcctc agtcggctct tcggcgagat gaggagttgt
      961 cgctatttca ggcggcagtt cacgaattct ctgtcgctct ggtaacggcg gggaaattgg
     1021 aatgtcttcg gtctccttgc tcagcgtctc gcttgtagga accactctat cgtctgtgag
     1081 cgattcgctc tcgaaaatcg gcgacgctat cgctgctatg ggaactacta acgccggcgg
     1141 ctcatccatc gtcagagagc tcgccggcgg cggctgttcc ggttttacaa aggtgaagtc
     1201 gctttccgtc tgaatggaga ccggtttcac atcggcagca ggaatctccg gctcaggcga
     1261 gaccggcgtc tgtagggctg gagcaatcga gaacgtctcc atttctgcag cgggactaac
     1321 acgaaagatc ggaggttgcg cttgatcgtc actaacttcg gatagcgact caacttcacc
     1381 ggccgatcgg tctaccgttg accccgctct ttcagctgcc ggctgatctg tttgcgaggg
     1441 aagaattgaa atgaccgact cctgaatttg atcccatggc atcggcgcaa ttggaaatcc
     1501 agaaggggtt tgttctccaa gcgatgtcgg ggcttcacgc tccggaattt caaccgctcg
     1561 gctttctaac gcaagctccc ggagaggtgg ctctgctgac gctggggtat gtacttcgtc
     1621 gacgtccgtc tgggtgacta ccggcaggga gggatttgct gaatgatcac ttccgaactt
     1681 ccatgcacta ttaaacacgg attcccatga gaagcctggt tgcttccatg gctctgaaat
     1741 ctcattggct gaaaccgact cgggactcga tggagcacgg acgtccggag gcggttcagt
     1801 aatcacttcc ttcgaagcgg tctcgacgga tttgtgtatg gagggttgcg catcctcgac
     1861 ctctatcggc ccggcagtcg gcggttcggt gtccaagtag gtggatgctg atatctcttc
     1921 aatctgggat gagggcgtta cgccggcagc ttcaatagac tgtgccgagc tagtcggagg
     1981 ttccgatgca tcggcggact cgatgggtgt ggaaaccgtg ggtgagccac ttacatctag
     2041 atcatagggt gcctccggct cagcctcggc cgacaatctg tcttgttcct gaactgcggc
     2101 aggaccacct gtcggcacgg ctggatccgg tccatgttgt gcctcggaac tctcggtagc
     2161 gggcacatca tttcgcactt cactcgcgtc gtcggctgtc tgctctcgta agggtggatc
     2221 ggatggcacc cagtctccat cacgggtcgg cccttgaggg gcaggaaggt ccgtgatcgt
     2281 cggagggata agagaccccg atgagtcgga tacatctgag tcggatacat cggctacttt
     2341 gaacgtctcc ggtgccgtcg cttgaaaatc ctgaatctcc cacggcatca ccccatccat
     2401 cggtttcgaa cctgcttgaa tactctgatc tccctcggac aggtcgggtg atggagcgat
     2461 ggtcgattgt ccggaatcgc cggactgccg agtcagcagc tcaccggttt gcgtgtcaaa
     2521 ggacgaagcc aatcgaaagg ccaccggact cgccggagca agttctcgta tctttgcgta
     2581 gagttgagcg gcatggtgtg gattgtcggg ctcagggtct tcgatcaaga tatcgattgc
     2641 cttgccgtac tcgccgacag cctcgagtgc atttcctttt tcttgataga gcgccccaag
     2701 tttctctagg aaaggaacac atcgagggcc ggcggctaag tattctcgga gcaacgattc
     2761 tgcgagccag taatcttgcc gctggaacgc ttcgctgatg agtgagtcaa aggcttgtct
     2821 cgccgggata aggcttccga gacgaaggtg gagggtagcg aaaagccgtc gcgcctccat
     2881 gttgtgcgga tcaagtgcca gtaattcctt cagcgcagca gaagaacgcc catattttcc
     2941 ggcattcatt tgggtgatgg cttgttcaag aagagccgtc ttcctgctgt agccgaccac
     3001 accacgcttg tgaccacggg tggtctggga tttatcagat tttaaagaga gttttttatt
     3061 cgagcgcacg ctacaacctt agcagatccc tctacctggg agcctgcatc agcggctcaa
     3121 tccgctctcc ccgactccct tgatgttggg catcgacata gcggtgaaac ctttgggaag
     3181 cctgcaatcc agatacctcg cgttctaatg attcattaga tcaagttatt gaaaattaag
     3241 gattgagtcg taagcattcc ttgcatttcc atcgtccatc caattttgga cagacctttg
     3301 ttcctcccgg cttggtcagt tggagagaac acaaaatcag tatccgaaga gcgaaacaga
     3361 tctatcagtt tatcggtttg cattacagcc gacctacgtc ggcttagtcc tcgcctggaa
     3421 cctctcttta tcggtttttt tgtgaaagta gttaactatc tgtaaagggc tcccatcaat
     3481 tcacgaaaga tcaaacggca aagacagacc tttctttcgc acatggaggt tcggctccct
     3541 ttccaaaaac ttcatccttg ttgtgtcctt cgatgtgcct atgatacggt caggcccaca
     3601 tatccagctc acttgatacc accacggaat caatagtcca tcgtgcaaac ccttcccata
     3661 ggttgtcgtc cgtcgaaaac gaccaggaga cttgaagcct acctagctgt gttactgttt
     3721 ttctccttga cctcccctcc tcctctattc tcgctgggac caggaggcca gccggcaaag
     3781 gtggccttag attttaatga agtggagatc cctgtcttcg tccgatttat cagtgagctc
     3841 acggggaaaa actttgttct ggacgaaacc attaaaaagc agggtggaaa aatctccgtg
     3901 ttttctccga ccaaggtttt accggaccag gccttcaata tgttcgtcgc ggcgttggaa
     3961 gccgcccgct tggctgtggt gccgaaaggg gggaacctct atcaggttgt gcccatgggc
     4021 gaccttccac cggagcgggg tgtgtttgta tacaaactca aacatgccaa tgcgacggat
     4081 ctagccgccg tgctgaccaa tctggttgca cgctcacaaa ccgttgcgca gatcacacca
     4141 ggcaccaggc ctcccatcag accgctgacg gaattcgaag cgccggtcca agtgtttgcc
     4201 gataaggcaa ccaactccat cgtcatcagt tcgaccaaga tcgcctggaa ccatctccaa
     4261 ggagtgattc gtgacctcga tatacggcgg aagcaggtct ttgtggaggc ggtcatcctg
     4321 gaggtggaag tggaccgcct ccgccaaatc ggcaccgatc ctactcaggt tctcggtgca
     4381 ggaaagtctg ggtttgtgca aggaatagga gggttcaaca gagcaccgga agaccttgca
     4441 accgtggctc aggccatcag cggcgtggcc gcgggcggag cgaccggtgg cgcagccacc
     4501 gttttgaaca cgctcaatgt tcgcgccttc atgaacttgc tgttgagcct cacggatacc
     4561 aatattctct ccacacccca ggtcctcgct gcggataacc aaaaggcgaa gatcgtcgtc
     4621 ggtgaaaatc gcccgttccc gaccggacag gcgcaaggta tcaccggtgg aaccctcgta
     4681 acaattgaac ggaaagacgt cggcgtgacg ttagagttga cacctcaagt gctcgaagac
     4741 gacttgatcc gccttgaaat caagcaagag attacggcca tcgagaccaa tgaggctcaa
     4801 acgatcggct caggctcagc aa
//
LOCUS       DATNDD010000470         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_814040, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000470 DATNDD010000000
VERSION     DATNDD010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814040"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VN647_11380"
     CDS             complement(<1..785)
                     /locus_tag="VN647_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HWG97677.1"
                     /translation="MTASPIRLGISRCLLGDEVRFDGEHKRDHFLTDILSRYVDWIPV
                     CPEVEAGLGTPREAMRLVGKTRRPRLMTITSKHDHTEVMETMIKKRLTSLDKLDLSGF
                     VFKKGSPSCGVKRVPVYTEQSMTTRRGSGIFAQAFQKQFPLIPVEDEGRLCDPALREN
                     FIERVFCYRRFQDLLQNGVTRQALISFHTVHKYLLLSHSQQHYETMGRLVGQADRYRP
                     KELTAKYGELFMNTLALKATVRKHVNVLHHMAGYFKNRLKALE"
     gene            complement(853..1791)
                     /locus_tag="VN647_11385"
     CDS             complement(853..1791)
                     /locus_tag="VN647_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HWG97678.1"
                     /translation="MNIVVSGGTGFVGRALCAALFHGGHRVSILTRNAGQDFHWPGMN
                     ANPVSWNGQDTGPWEQVLEEADAVINLAGAPIADAPWTDVRKQLITDSRVLTTRLLVR
                     ALSRCSSKPATFISASGIGFYGASDDRRLDEGTARGQGFLADLCLAWEAEALRAAEFG
                     TRVVLLRTGMVLERDGGALPKMLLPFRLFAGGPIMPGNQWISWIHRRDHIGLIQWALT
                     TATVFGPINAVAPEPVMMKTFCEILGRVVHRPSWLPVPEFALNLFLGELGTLMTTGQR
                     VIPAKATAGGYTFQYPTLEPALKAILIGTTIPQQAA"
     gene            complement(1862..2365)
                     /locus_tag="VN647_11390"
     CDS             complement(1862..2365)
                     /locus_tag="VN647_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HWG97679.1"
                     /translation="MLAYTQIKHQIQGQEEVIATVIKNWQQAHPAAQRFVILPDFNNA
                     AVLDKDTNLIWELSPTPTHVTWNEARAACIIRTTGGQKGWRLPAPAEMRSLVGPAVDS
                     PIPNIPPGHPFLNIQPTSYWTVVPEANQPSYARYVDAFLGNVLSFIKIYTYPVWCVRG
                     PIKQDDQ"
BASE COUNT          672 a          756 c          807 g          666 t
ORIGIN      
        1 ttttcgagag ccttcaatcg attcttgaaa tagcccgcca tatgatgcag cacgtttacg
       61 tgcttgcgga ctgtcgcctt cagggctaaa gtgttcatga agagttcacc gtacttcgcc
      121 gtcaattcct tgggtcgata acgatccgcc tgaccgacca gccggcccat tgtttcataa
      181 tgttgttggc tgtgggacaa gagcagatac ttatgtacgg tgtggaagct tatcagagcc
      241 tgcctggtga ccccgttctg cagcagatct tggaatcggc gatagcaaaa gactcgttca
      301 atgaagttct ccctaagcgc cgggtcgcag agacggcctt cgtcttcgac gggaatgaga
      361 ggaaactgct tttgaaaagc ctgcgcaaag atcccagagc cacggcgggt cgtcatgctc
      421 tgttcggtgt acacaggcac ccgtttgacc ccgcaactgg gcgatccctt cttaaagacg
      481 aatcctgaga ggtccagttt gtcgagcgag gtgagacgct tcttgatcat cgtctccatg
      541 acttcggtgt gatcgtgttt gctcgtgatc gtcatcagcc gaggacgccg cgttttacct
      601 accaaccgca ttgcttcacg cggggtgccc agtcccgctt caacctcagg gcagaccggt
      661 atccagtcaa cgtagcggct cagtatgtcg gttaaaaaat ggtcgcgctt gtgttcaccg
      721 tcgaagcgaa cttcgtcgcc gaggagacac ctgctgatgc ccaagcggat tggagaagcc
      781 gtcattccgt cacctgcacc atgagcccac ctgagacaat cttgctaagg agattcgtcg
      841 ttattccgca actcatgctg cctgttgggg gatggtcgtt ccgatcagga tagcttttag
      901 cgcgggctcc agtgtcggat actgaaatgt ataacctccc gctgtcgctt tcgcaggaat
      961 cacgcgctgg ccggttgtca ttaatgtccc caactcacca aggaaaaggt tcagggcaaa
     1021 ctctggaacg gggagccatg atggtctgtg aaccacccgc ccaaggatct cacagaacgt
     1081 tttcatcatc actggctcgg gggccacggc attgattgga ccgaaaaccg ttgcagtcgt
     1141 gagcgcccat tggatcagac cgatgtggtc acgccggtgg atccaggaga tccattggtt
     1201 tcccggcatg atcggacctc ctgcaaataa tcgaaagggc aacagcatct tgggtaaggc
     1261 tccaccatct cgttcgagca ccattccggt tcgtaagagg accacacgcg taccgaattc
     1321 tgcagcccga agggcctccg cttcccatgc gagacacaga tcggccaaga agccctgacc
     1381 gcgcgctgtt ccttcatcca gacgacgatc gtcgctggcg ccataaaagc cgattccgga
     1441 agcgctgatg aaggttgcgg gtttggaaga acagcgagac agcgccctga ccagtaagcg
     1501 ggtggtgagt acacgactat cggtgatgag ttgttttcgt acgtctgtcc agggtgcgtc
     1561 ggcaataggg gcgccagcga ggttaatgac cgcatctgcc tcttcaagaa cctgctccca
     1621 aggtccggta tcttgcccat tccacgagac cgggtttgcg ttcatcccag gccaatggaa
     1681 atcctgtccc gcatttctcg tgaggatgct gactctgtgg cctccgtgga ataacgctgc
     1741 acacaatgcc cggccaacga accccgttcc tccggatacc acaatgttca taggcctatt
     1801 tcctgagccg gcggcaaggc tagtcaatca agcggaatct ccataccgcg cccagcgcaa
     1861 ctcattgatc gtcctgcttg atcggcccac gaacgcacca gaccggatac gtgtatattt
     1921 tgatgaagct cagtacatta ccaaggaatg cgtccacgta tcgcgcatac gatggctggt
     1981 tcgcttccgg gaccaccgtc caataggatg tgggctggat gttcaagaat gggtgacccg
     2041 gcggaatatt cggaataggg gagtccactg ctggtcccac gagactacgc atttcggccg
     2101 gcgccggaag acgccagccc ttttgaccgc cggtcgttcg gataatacag gcagcacggg
     2161 cttcgttcca tgtcacgtgc gttggtgttg gtgagagttc ccagatgagg tttgtatcct
     2221 tatcgagaac tgcagcgttg ttgaagtcgg gcaagatcac gaagcgctgg gccgctggat
     2281 gcgcttgttg ccaattcttg atgacggtgg caatgacctc ttcttgccct tgtatctgat
     2341 gcttgatctg cgtgtatgcc agcaagaaaa acgcacctat cgcgactatc gcaacgaaaa
     2401 ttcctcgcga gctggcccat ccagaaggtc tcatactgct tccgacgaaa tgacgaaata
     2461 aagtatccta cgctgtggac tgcaaaagga tcaacatttt cagagtggga ctttgctgga
     2521 gcaagtggag cctccacggc ttgcgccatc ctcagccgac tactgcggag gacggcctag
     2581 gcgggtgaag atagataaac ttggggaaaa gagtaatgag aaaaggtcta agcggatgac
     2641 ccataaccta ctgaaatgaa agcgattagt tccgaatgag ggaatgaaat tatcagttgg
     2701 cattggtcta tggatttgag gtagtatcgg tgatgattaa gtgattataa gactgagatt
     2761 tggagagctg gtatcggttt agtaaggatg acagttaggc gaatgcgagt gatgagtcaa
     2821 taagctaaag ggaggtcaca ctatggcaag cgatagaggg tatgacatct cgcagtggta
     2881 cgactcgaag ccggtgaagc t
//
LOCUS       DATNDD010000471         6775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2494592, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000471 DATNDD010000000
VERSION     DATNDD010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494592"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..2450)
                     /locus_tag="VN647_11395"
     CDS             complement(165..2450)
                     /locus_tag="VN647_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWG97680.1"
                     /translation="MYLVRAINRLNEAKTMNRWKLYKRILRTLFVAVPFLAASSIQDA
                     AGYLSRTPTAFSEDGRILLVQMEELVVWDLETKALVAKIPGLHCEQVALLKQEGWVLC
                     VEHSVTIYDWKNRVSVATIPPESQQPDSLLAYSKETDRMILRHGNEAVSVWQLGKKPV
                     PIKHISLEGKTDISSVAASPDTKLLAIAQGHTIHLHDLNGTTVRDIVIEGGKPHDLLF
                     APNSSRLAGSSGKMIFLIDPVDASIAVRATLTSAEKAQGSLTPEVFSRDGHRLVAANG
                     EWGYALFKTDTGELVSLTEFTYAAPGRGMRSPTQLRAVDIAADADLLVGQPEHLHTLQ
                     IWDLQTGAMLPDLCGEDCRNMGLRVSLLKWSPIGSKIVVGMQGALNPDVDGKISIWDV
                     PSRSPELVLDSGQPQAKVLAKRATPPTFITMTSFPPAAPAFAHARALRAVATSPSANL
                     LVTSGDDGLLKIWDPGQGALLRQLVLTAPAHALAFSADGAILAAGTMEGDVRLWETRT
                     WREFSPYSSRQGRINALQFIPGNRFLVVAGGEPRVLVMDLVTRAVVRELVHTSRYPAC
                     DEKRCIKKRAVRREAVDSLSFLDGSSFLLTASQSGSVVWDITTWREVEKPAGLPDAWS
                     GLGWKRPFVWTTTRPGVPTAFTLAIWDTKNNKVLASLDTFTHRDTEIDHGPAVTLGTS
                     MAVDPSHRWAATRVGEHISVWDLSAQTKQKTFHVKTPYHLHWTSDGNYLIVATLDRKI
                     LVWSAETMEPAHYLRDPSVTR"
     gene            complement(2416..2967)
                     /locus_tag="VN647_11400"
     CDS             complement(2416..2967)
                     /locus_tag="VN647_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWG97681.1"
                     /translation="MKVLTLGLMSGLLFWPVIGSAELYKWSDDQGNLNITDMPPPSTQ
                     KKSATTVTPVPRSASPKKVTVRPTLPGRPQAEIHPVPGSSGPSPATEEVLIPRPMEGL
                     RPNQATLTSLWEIFNGTQMNAKAPVQRWKDEQGLDHFVDVLPATLGRSEVAPKGEDVS
                     ASPTGKTKERVTGVSRSRHQSTK"
     gene            3435..4013
                     /locus_tag="VN647_11405"
     CDS             3435..4013
                     /locus_tag="VN647_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWG97682.1"
                     /translation="MAQALTSEDVQQAWSALIEDTRTAVLLTMRNGRPFGSHVPYVFG
                     SDWTNAYLHLSRLALHTQHLLADPHVSLFIAETDGPEKNPLALRRINLMGQAAPLSPD
                     TPSYGEFKERYLSRFPQAEMMFGFGDFALWEFGLKTPTLCSASVKPSSRPSPLHPDGC
                     IKKWNDPLRGRTDLTSKSSAREYTRLAQLTVK"
     gene            4207..4458
                     /locus_tag="VN647_11410"
     CDS             4207..4458
                     /locus_tag="VN647_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97683.1"
                     /translation="MKPPPPRFVPYEVLVKFKDGISQERIASILKDNRIDVIAEIQRG
                     RLYHARILDDRSVESAITQLTSYQEIEYAEPNYRYETQK"
     gene            4421..6775
                     /locus_tag="VN647_11415"
     CDS             4421..6775
                     /locus_tag="VN647_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWG97684.1"
                     /translation="MQSPIIDTKRRSSVMRIALCSGGTSWGMAMVLVALSGLMPGDRL
                     VHAEQKQASRGMTALSQDARHPARYVPGEVLVKFRDEASSSGIQSLNMVAGAKEIRVL
                     SMNARMIHQYKLEDTLSVDEAVLKYRSNPAVEYAEPNYLYWLNATPNDSRFGTLWGLH
                     NTGQVVNGTAGRADADIDAPEAWDISTGSPNVIVAVIDSGIAYDHPDLAPNIWTNPGE
                     IPGNGVDDDGNGLVDDVHGWDFRMNDGDPIDPIALNPDGNPGHGTHVAGIIAGAGNNG
                     IGVTGVMWTAKLMALRAGGVDRSVSTTAIVSAIHYAVDNGARVINASFGGPDCSLALY
                     DTVNAANAAGVLFVAAAGNGGSDNDNVPNFPSNFSAPSVCDGQQKAALSNVIAVAATD
                     QNDELAIFSSTAASNFGANTVQVAAPGVSINSTKPTSNVTNVLLHNFDSNPTGLGYVF
                     GGTNNTWGFTNSTSFSQPNSLSDSPNGNYQNNTDSFAIGPVFSTEGQRGCLLDSRVRL
                     QTDGIDGVVVETSRNSGASWQPIRGVTEITNAPFDPFTWVDVADGVAGSRFRFRFISD
                     EGQVFDGVYVDDVRVACVAGPPSGTTDYQFLQGTSMATPHVAGLAGLLLSVDPNLTVS
                     QLRNAILNTVDRKASLRGKVSTGGRINARAALASVVANFAVTVNKAGAGTGTVTSNPS
                     GIDCGTTCNGQFLQGRTVNLTAVPDPGSVFAGWSGDCSGTDPCSLTQNTTVTATFNIA
                     PTPSVPGNDAGGGCTLAPGATGDILLPVMLLMSLIILLWRARRR"
BASE COUNT         1565 a         1868 c         1870 g         1472 t
ORIGIN      
        1 tgcgcgaata gacctaccag cgctttcacc agcacaagct acaacaccag tcatcagcag
       61 actacgttcg gcacgagtgc agaaagctgc atgacaatct gacccagaac agatgccggt
      121 tcctgagcca tccgtataga gcaaccgcaa tggtttatga cgtatcagcg ggtaactgag
      181 ggatctcgca ggtagtgcgc cggctccatg gtctcggccg accagacgag gattttacgg
      241 tcgagagtgg ccacgattaa atagttgccg tcgctcgtcc aatgaagatg gtacggcgtt
      301 ttgacgtgga acgtcttttg ttttgtttgt gccgaaaggt cccagactga aatatgttct
      361 ccgactctgg tggcagccca tcgatgcgat gggtcgacgg ccatcgatgt ccctaacgtc
      421 acggccggcc catgatcaat ttctgtatcc cggtgggtga aggtatccaa gctcgccaac
      481 actttattgt tcttggtatc ccagatagcg agggtaaagg cggttggaac accggggcgg
      541 gttgtcgtcc agacaaatgg acgtttccag ccaagaccgg accaggcatc cgggagaccg
      601 gccggcttct caacctcgcg ccaggttgtg atgtcccaga cgacagaacc ggattgcgag
      661 gccgtcaaaa gaaacgagct tccatctaag aagctcaaac tgtctacggc ctctcttcgt
      721 accgctcgct tttttataca gcgtttctcg tcgcaggccg gataacggct ggtatgcaca
      781 agctctctca cgaccgctcg ggtgacgaga tccatgacaa gaactctcgg ttctcctccg
      841 gcaacgacca gaaatcgatt gccgggaatg aattgcagcg cgttgatccg accttgcctg
      901 cttgaatagg gtgagaactc acgccaagtc cgggtttccc acaaacgtac gtctccttcc
      961 atggtaccag ccgccaagat cgcgccgtcg gcgctgaacg caagcgcatg tgccggagca
     1021 gtcaatacca gttgacgcaa gagagctccc tgccctgggt cccatatttt cagcagtcca
     1081 tcgtcgccgc tcgtgacgag cagattcgcg ctaggagacg tcgccactgc gcgcaaagcc
     1141 cgtgcatggg cgaaagccgg cgcagccggg ggaaaagatg tcatcgtaat aaatgttggt
     1201 ggggtcgctc gtttggccag taccttggcc tgaggttggc cggagtccag caccaattcc
     1261 ggggaacgtg agggaacgtc ccaaatcgat atcttcccat ccacatcagg attgagggct
     1321 ccctgcatcc caaccacgat tttcgaacca atcggcgacc atttgagtaa cgaaacgcgg
     1381 agacccatat tgcggcaatc ctctccgcac agatccggca acatcgcccc ggtctgtaga
     1441 tcccagatct gtaatgtgtg aagatgctcc ggttgtccga ccaagagatc tgcatcggca
     1501 gcaatgtcga cggcacgaag ctgtgtcggt gagcgcatcc cgcgccctgg agctgcatag
     1561 gtaaactcgg tcaaagacac cagctcgccc gtatcagtct tgaaaagcgc atagccccat
     1621 tccccattgg ctgccaccaa acgatgaccg tcccgcgaga agacttcggg cgtgagggag
     1681 ccctgtgcct tctcagcact cgtcaatgtt gcacgcacgg ctattgacgc atccaccgga
     1741 tcgatcagga aaatcatttt gcccgaactc cccgccaaac gagaactgtt cggcgcaaag
     1801 aggagatcat gcggtttgcc gccttctata actatgtcac ggacggtggt cccgttgagg
     1861 tcgtgcagat gaatggtgtg gccttgcgca atcgccaaca gcttcgtatc gggcgaggcg
     1921 gccacggaag agatgtctgt cttcccctcc agcgaaatat gtttgatcgg tacgggcttt
     1981 ttccccagct gccagacgga cactgcctca ttcccgtggc ggagaatcat ccgatcggtc
     2041 tctttcgaat aggcaagcag gctatcgggc tgctgcgatt ccggtggaat ggtggccact
     2101 gagactcggt tcttccaatc gtagatggtg acgctgtgct ccacacacaa cacccacccc
     2161 tcctgcttca gcaacgcgac ctgctcgcag tgaaggcctg gaatcttcgc cacgagcgcc
     2221 ttcgtctcaa gatcccagac aaccagctcc tccatctgga ccagcagaat ccgcccatcc
     2281 tcagagaatg cagtgggggt tctagagaga taaccagccg cgtcctgaat cgatgaggcg
     2341 gcaaggaagg ggacggcaac gaatagggtc ctcagtatcc tcttgtacag tttccaacga
     2401 ttcatggtct tcgcttcatt tagtcgattg atggcgcgaa cgagatacac cggtcacccg
     2461 ctccttggtc ttacctgtag gggaggcaga aacgtcttca ccctttggcg caacttctga
     2521 gcggcccaga gttgccggca agacatcgac gaagtgatca aggccctgtt catccttcca
     2581 ccgctgaacg ggagccttgg cattcatttg ggtgccgttg aagatttccc acaaactcgt
     2641 cagcgttgct tgattcggcc tcaaaccctc catgggccgt ggaataagga cctcttcggt
     2701 tgcgggagaa gggcctgagg aaccaggtac gggatgaatt tcagcttggg gtcgcccggg
     2761 caaggtcggc cgaactgtga ccttcttcgg cgatgcggat cgaggaaccg gcgtcacagt
     2821 cgtcgccgac ttcttttgtg tcgaaggagg cggcatatcg gttatattga gattcccttg
     2881 atcatcactc catttataca gttccgcgga cccgataacc ggccagaaca gaagaccgct
     2941 catgaggccc agtgtcaaaa ctttcatatt gtgagatgcc ctcagtatcc aaatgatgtt
     3001 gtagttgcca aaagtatagc aggcagtccc atttatgcct gggcgtaccc gggcacccgc
     3061 gcatggccgg cactcggtcg gaagcagaaa agaagtccgg gattggagtg ggagttggat
     3121 cggacgctag ggtgggagga gcacgcacct aaccgaggac gatcaggtac ggcggagatc
     3181 ttgggaggac tttaggcttc ccagtacaag ctgggcctgc acaccatcct cgcgctcgat
     3241 cccctgttga accatattcc ctcggggcgt taactcctcc cacctgcgta cgataggcct
     3301 caactatgaa tcagtcaaga aaactaatgt gtcgttcctt acccggttca cgaaagtcgg
     3361 ggatacatcc ctcttgacgt ttgaaatcgt aacgtgcagg gtgctctccc tctacaattc
     3421 ttgaaaggtt ggcaatggct caggcactca cgagtgagga cgtacaacag gcatggtcgg
     3481 cactcataga agatactcgc acagccgtgc tgctcacgat gcgaaacgga cggccatttg
     3541 gatcgcatgt gccctatgtg tttggatcgg actggaccaa cgcgtatctg cacctcagcc
     3601 gactggcgct tcatacgcag catctgctgg ctgatcccca tgtgtcgctg ttcatcgctg
     3661 agacggacgg accagagaaa aatcctctcg cgctaagaag aataaatctt atgggtcaag
     3721 cggcaccgtt gtcacctgat acaccgtcgt atggcgagtt caaggagcga tacctctcgc
     3781 ggtttcctca agccgagatg atgttcggct ttggtgattt tgctctctgg gaattcggct
     3841 tgaagacgcc cactttgtgc tcggcttcgg tcaagccttc atctcgaccg tcgccactcc
     3901 atccagatgg gtgcatcaaa aagtggaacg acccactgcg aggaaggact gatctgacct
     3961 cgaagagcag cgctcgcgaa tatacacggc tggctcaact taccgtcaaa taaattacaa
     4021 aaccccaaca gatatgtgac tcgccgcctg cttcagagag agaaaacgat gatgtacttg
     4081 tccattctat ctcgtcggat gacacaactt gtcacaatca tgatgtgtgt gtcggctata
     4141 tggttcttca catcctgtga cgcaaggaac cagaatacga caacgacaca gacctttgaa
     4201 cgccctatga aacccccgcc accacgattc gtcccatacg aagtgttggt caagttcaag
     4261 gacgggattt ctcaagagag gatcgcctcc attctgaagg ataaccggat cgatgtgatc
     4321 gctgaaattc aacggggacg cctctatcac gcccgcatct tggacgatcg gtcggttgaa
     4381 tccgcaatca cccaactcac gtcatatcag gagatcgagt atgcagagcc caattatcga
     4441 tacgaaacgc agaagtagtg tgatgagaat tgcgctctgt tcgggcggga ccagctgggg
     4501 gatggcaatg gtcttggtcg cattgtcggg actcatgccg ggagaccgct tggttcatgc
     4561 ggagcagaaa caagcatctc gcggaatgac ggcgttatca caggatgcgc gccatccggc
     4621 ccggtatgta ccaggagagg tgctcgtcaa gttcagggat gaggcgtcct cgtcaggaat
     4681 ccagtctctg aatatggtgg cgggcgccaa agaaatcaga gtgctgtcta tgaatgctag
     4741 gatgattcac cagtacaaat tggaagatac cttgtcggtc gacgaagcgg tcttgaaata
     4801 ccgaagtaat cccgctgttg aatatgccga accaaactat ctctactggt tgaatgcgac
     4861 ccctaatgat tcgcggtttg gcacgctgtg gggtcttcac aacaccggtc aggttgtcaa
     4921 tggaacagca ggaagggcgg atgccgacat cgatgcgccg gaagcctggg atatcagcac
     4981 gggaagtcca aacgtgattg tcgccgtcat tgactcgggt attgcctatg atcatccgga
     5041 tctcgcgccc aatatctgga cgaatccggg agaaatccct ggcaatggag tcgatgacga
     5101 tggaaatgga ttggtagacg atgtgcatgg atgggatttc cgcatgaacg acggtgaccc
     5161 gattgacccg atcgctctca atcccgatgg taacccggga catggcactc atgtcgcggg
     5221 aatcatcgct ggagccggaa acaatggaat cggggtaaca ggggtcatgt ggaccgcgaa
     5281 gctgatggcc ctaagagccg gaggggtcga tcggtccgta tccaccaccg ctattgtcag
     5341 tgcgatccac tatgccgtcg acaatggcgc acgtgtcatc aacgcgagtt tcggcggacc
     5401 tgactgtagc ctggctctct atgatacggt gaatgccgcg aatgcggcag gcgtactgtt
     5461 tgttgcagca gcaggaaatg gtggatcgga taacgacaac gtaccgaact tcccctcaaa
     5521 tttcagcgct ccttcggtat gtgacgggca acagaaggct gctctctcga acgtgatcgc
     5581 cgtggccgcc acggaccaga atgacgaatt ggctattttc agttccaccg cggcgtccaa
     5641 ttttggggct aacacggttc aggtggccgc gccgggagtc agcatcaaca gcacgaaacc
     5701 cacgtcgaac gttacgaatg tgctcctgca taacttcgat tcgaacccaa ccgggctcgg
     5761 gtatgtcttt gggggaacca ataatacctg ggggttcacc aattccacat cattcagcca
     5821 accgaacagt ctgagcgaca gtccgaacgg aaactatcag aacaacaccg attcattcgc
     5881 gatcggacca gtgttcagta ccgagggaca gcgggggtgt ctgcttgata gccgcgttcg
     5941 cctgcaaacc gacggtatag acggtgttgt tgtcgaaacg tcaagaaaca gcggagccag
     6001 ttggcagccc attcgtggag tcacagaaat taccaatgcc ccatttgatc ctttcacctg
     6061 ggtggatgtc gcggatggtg tggctggttc acggttccgc ttccgattca tatcggatga
     6121 gggtcaggtc ttcgacggtg tgtatgtaga cgatgtccgc gtcgcctgcg tggcgggtcc
     6181 gccatcggga acgacggatt atcagtttct tcagggtact tcgatggcca ctccgcatgt
     6241 ggcaggactc gccggcttgt tgctctcggt tgatcccaat ctcaccgtat cacaactcag
     6301 gaacgcaatc ctcaacacag tggatcggaa ggcttcctta agaggaaagg tgtccaccgg
     6361 tgggcgcatc aatgcccgag ccgcattggc cagcgttgtg gcaaacttcg cggtcacggt
     6421 caacaaggca ggcgccggaa ccggcacagt gacatccaat ccatcaggaa ttgattgcgg
     6481 aacgacatgc aatggacagt ttctccaagg tcgcacagtc aacctcaccg cagtacccga
     6541 tcctggatct gtcttcgcag ggtggagtgg agattgtagt ggaactgatc cctgctcgct
     6601 cactcaaaat acgactgtaa ctgcaacctt caacatcgcc ccgaccccgt cagtccccgg
     6661 taatgacgcc ggaggaggct gcactctcgc cccgggcgcc actggagaca tattgctgcc
     6721 tgtcatgcta ctgatgtctc tgattatctt gttgtggagg gcaagacgcc gatga
//
LOCUS       DATNDD010000472         4092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3963722, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000472 DATNDD010000000
VERSION     DATNDD010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3963722"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VN647_11420"
     CDS             <1..782
                     /locus_tag="VN647_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97685.1"
                     /translation="FHEYPLLKEKVGIVLVKGVLKEDQEGVDILRKWGSVEKGLAQEF
                     EAKGMKGVGIKLIPRRFYDPALTRYCRHELMHISDMIDPQFGYDPDTKVGLNPGEETL
                     ILQRYRVLWSLSVDSRLITAGKEPMLSKEERFKEFRSWYRKIPPPQLKSVFEGLWQTS
                     YFTHSELIDMAAETLRVMDRAVDVEGGEVPETENKIMLMPGFPCPLCRFPTYSWVEDM
                     GTTVESYVLDFIRENHPGWDVDFGACDRCVEVYKLRADGVM"
     gene            972..1574
                     /locus_tag="VN647_11425"
     CDS             972..1574
                     /locus_tag="VN647_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWG97686.1"
                     /translation="MATNPSYGRRDFLKDSVISTVKAARELAKQADGLPAEPPPPPTR
                     IDWLRPPGAAGEQLFLERCTKCNDCVTACSPGAIVAHPQDGTPVLFADQSPCLLCEDL
                     PCIAACGTDALLPVMGVNQVHMGIATISHRFCTAGQGCHACVSKCPTDALVVDFASLH
                     LSVVEEACVGCGMCEMVCKTVNDHIAIRVVPARHLARIDS"
     gene            1731..2702
                     /locus_tag="VN647_11430"
     CDS             1731..2702
                     /locus_tag="VN647_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HWG97687.1"
                     /translation="MTNQQPMQGVSASATATLPNLSTIKDSPAVERALNRSKIYLLIS
                     WSLLYPEDEEFLDYLRSGEFVEDGRAALAALESALGPDGSGRAKEKLGSLKQQFGWVE
                     GLISSECVNWQLGDLQSEHRRVFSNVITLDCPPYETLFGNDHVFAQSHVMGDISGFYK
                     AFGVELSKDIHERLDHLSVEFEFMHFLAYKESYSLCHDGPEKTQIVVDAQKKFVKNHI
                     GRWVPLFCRMLTKKADSGLFKLVADMTADWMEFETVFLGVTPQPYTETDYRPATFSSP
                     EGQTYECGAQDQGNELTMLLNEVGAQSFMDVKEKDKGQEEGGPSGTA"
     gene            2765..3715
                     /locus_tag="VN647_11435"
     CDS             2765..3715
                     /locus_tag="VN647_11435"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HWG97688.1"
                     /translation="MRVAQTTNKKLVFAILLSAITVGLMLTLGRVPLAVSQPVTIPAK
                     AIKGSIPMDGANPIWESVPGVVVPLSGQLITTPMHPNISVKSVFVKAMTNGKDIGLRL
                     EWSDQTKNDTAIGPQDFRDQVAVMFPVSTAGAPPFQCMGQSGGTTNIWRWNAEWQKDL
                     GKDSAGIWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNPGIWSGNIMSDPTL
                     RVSSVEDLNANGFSTLTTQAHQNVIGNGVWEPAGSVKGGGYTGPTWRVVVKRTLESGD
                     ANDVQFKPGISVPIAFAVWDGANIERNGMKSLSTWFTLKL"
BASE COUNT          915 a         1026 c         1220 g          931 t
ORIGIN      
        1 cgtttcacga gtatccgctg ttaaaggaga aggtgggaat cgtcctggtc aaaggtgtgc
       61 tgaaggaaga tcaggaaggc gtcgatatcc tcaggaaatg ggggtctgtt gagaaaggct
      121 tggcgcagga gtttgaggcg aaagggatga agggcgtcgg gatcaagctg attccccgcc
      181 gattttatga cccggcgctg acgcgctatt gccgccatga gttaatgcac atctccgata
      241 tgatcgatcc gcagttcggt tacgatcctg ataccaaggt ggggctgaat cccggcgaag
      301 agacgttgat cttacagcgc tatcgtgtgc tctggagttt gagcgtggat agccggctga
      361 ttacggccgg caaggaaccc atgctgagca aggaggaacg attcaaggaa ttccggtcgt
      421 ggtaccgaaa gattccaccc cctcagctga agtcggtgtt cgagggactc tggcagactt
      481 catacttcac tcattcagag ttgatcgata tggccgccga gacgcttcgt gtgatggatc
      541 gggctgtaga tgtggaaggc ggcgaggtcc cggagaccga aaacaaaatc atgctcatgc
      601 cgggttttcc ctgcccgctg tgtcggttcc ccacctattc gtgggtcgag gatatgggaa
      661 cgacagtgga gtcgtatgtg ctcgacttca tccgcgagaa tcatccgggg tgggatgtgg
      721 acttcggagc ctgtgatcgc tgtgttgagg tgtataagct gcgtgccgat ggggttatgt
      781 agatgaggag ggtgaaaaag gccgccggct gcgttctcgg ctcgcaagga tcctcaacgt
      841 accgacctcg ggaaagaagc tgctccagca gctcggggcg ggcgggtgag aaaaggtacg
      901 cctgcggtcc ttgcatcacc tgtggccttg ccgaacgacc tttttgatcc tcctccaagg
      961 aatccatacc cgtggcaacc aacccttcat acggccggcg agatttcctg aaagattccg
     1021 tcatttctac tgtcaaagcg gcgcgcgaac tcgccaaaca ggcggatggt cttccggcgg
     1081 agccgcctcc tcctccgaca cgtattgact ggctgcgccc ccctggtgcg gcgggagaac
     1141 aactgttcct tgaacgttgt acaaagtgca atgattgcgt cacggcctgt tcacccggcg
     1201 cgatcgtcgc acatccgcag gacggtaccc cggttctttt tgccgatcaa tcaccctgtc
     1261 tcctctgcga agacctcccc tgcatcgcag catgtgggac ggatgcgctc ttgcccgtca
     1321 tgggggtcaa tcaagtccac atgggaatcg ccacgatttc tcaccgattc tgtacggcgg
     1381 gccagggatg tcatgcgtgc gtgtcgaagt gtccaacgga tgcccttgtc gtggattttg
     1441 cgtcgttgca tctatcggtg gtggaggaag cctgtgtcgg gtgtggaatg tgtgaaatgg
     1501 tctgtaagac ggtcaacgac catattgcaa tccgggtggt accggcaagg catttggcaa
     1561 ggatcgattc atgatggtgt cgctccgccg agatcgctcg gcagaaaatc tcaattcgca
     1621 acatcgaacc aattcactct tgacttcgta ccgcccttat tcatatacat acatggctct
     1681 gttgctaatc tcgggggttg ggtgaacgag acggccagga gacgaccact atgacgaatc
     1741 aacagccgat gcaaggggta tctgcatcag caaccgccac gctgccgaat ctttccacca
     1801 tcaaggactc tcctgccgtc gagcgtgcgc tgaatcgcag caaaatctat ttactgattt
     1861 catggagtct tctgtacccg gaggatgagg aatttctcga ttacctccgg tccggagagt
     1921 tcgtggagga tggtcgggct gctctcgctg cattggaaag cgctctcggt cccgatggca
     1981 gtgggcgggc caaggaaaag ttgggctcgc tcaaacaaca atttggttgg gtggaaggcc
     2041 tgatctcttc cgaatgtgtc aactggcagc ttggcgatct tcagtcggaa caccgccgcg
     2101 tgttcagtaa cgtgattacg ctcgattgcc ctccttatga gactcttttc gggaatgacc
     2161 atgtgtttgc gcagtcccac gtgatgggcg atatctccgg cttctacaaa gccttcggag
     2221 tcgagctctc gaaagatatt catgaacgcc tcgatcacct cagcgttgaa ttcgaattca
     2281 tgcacttcct cgcctacaag gaatcctact cgctctgcca cgatgggccg gaaaagacgc
     2341 agattgtggt cgacgcacag aagaagtttg tcaagaatca tatcggccga tgggtgccgc
     2401 tgttctgtcg tatgttgact aagaaagcgg actcgggtct cttcaagttg gtggccgata
     2461 tgactgctga ttggatggag tttgaaacgg tattcttggg ggtgacccct cagccctata
     2521 cggaaaccga ctatcgcccg gcgacgttca gttctcccga aggccagacc tatgaatgtg
     2581 gtgcacaaga tcaggggaac gaattgacta tgttgttgaa cgaagtcggt gcgcagtcgt
     2641 tcatggatgt gaaagagaaa gataagggtc aggaagaagg gggaccttcc ggaaccgcat
     2701 gagaaaatcg gtaggggtat tcaataaaag gcaatcttca caaaaaagag gagggagcag
     2761 caatatgcga gtagcgcaga cgaccaacaa gaaattggtg tttgccattc ttctctccgc
     2821 catcactgtc ggcctgatgc tgacgttggg gcgagtaccg ctggccgtca gtcaaccggt
     2881 gacgataccg gccaaggcga tcaagggatc gatccccatg gatggcgcca accccatttg
     2941 ggagagtgtt ccgggcgtcg tcgttccgtt gagtggtcag ctcatcacga caccgatgca
     3001 tccgaacatc tcggtgaagt cggtattcgt caaggctatg accaacggca aggacattgg
     3061 gttgcggttg gagtggagtg atcaaacgaa gaatgacaca gcgatcggac cgcaagattt
     3121 ccgggaccag gttgcagtga tgttcccggt aagcacggca ggcgctccgc cgttccagtg
     3181 catgggccaa tccggcggga cgaccaacat ctggcggtgg aatgccgaat ggcagaagga
     3241 tctcggtaag gacagcgcgg gaatctggga tgtggacgat cagtacccgg gcattttctg
     3301 ggattattat tttgaagagc cggccggcgg cgtcacctat ccggaccgca tcggtcgaag
     3361 tctagggcca ttcaatcccg gcatctggtc tggaaacatc atgtctgatc cgacacttcg
     3421 tgtgagttcg gtggaagatc tgaacgcgaa cgggttcagc accctcacga cacaagcgca
     3481 ccagaatgtc atcggaaacg gggtctggga accggcggga tccgtcaaag gcggagggta
     3541 taccggtccg acatggcggg tcgtcgtgaa gcggacgttg gaaagcggcg atgcaaacga
     3601 tgtgcagttc aagccgggga tatcggtgcc gatcgctttt gcagtgtggg atggtgccaa
     3661 tattgagcgg aacggcatga agtcgctttc gacgtggttc acgttgaaac tgtaatgacc
     3721 tgagcggcat tcgagccgat agcaacagcc gccacgttgt gtcattctaa agaggcctcg
     3781 gatcgggatc ccggtaagtg agggaacggt tcgaggcctt tttttctgga agattccggt
     3841 attggtttaa gcctcgctac actcgcagcg attgcatttc gcgaagtgcg gagggtataa
     3901 gaaaaagata tctcgaacca actgattttc aagcataaaa ggacaagcat cgcatgaaag
     3961 tttcgctgct ttttccccca acctggcatc cttctcaacc ctatctcagc ttgccgtccc
     4021 tgaccgggtt tttgcgtcag ggtgggattt ccgacgtctc gcaacgcgac cttggtatcg
     4081 aattgttgga ta
//
LOCUS       DATNDD010000473         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1465619, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000473 DATNDD010000000
VERSION     DATNDD010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465619"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VN647_11440"
     CDS             <1..650
                     /locus_tag="VN647_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HWG97689.1"
                     /translation="HDETHALALTLQTAKPLVTHGAAGISRKGKDVGQASHYYSFTRL
                     ETSGKLTIDGESFDVTGTSWMDHEFGSTELGADQVGWDWFSIQLEDDTELMLYRMRRK
                     DGSSDLASSGTAVSPDGRTRHLEVTDFQIESTGTWTSPESKATYPSKWRLKFPSLDLV
                     LDVTPLVADQELRTSRSTKVSYWEGAVAVTGTKQGRPMKGHGYVELTGYAERLKM"
     gene            complement(663..935)
                     /locus_tag="VN647_11445"
     CDS             complement(663..935)
                     /locus_tag="VN647_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97690.1"
                     /translation="MIKKRGRTKLPESGTLLVPSSALLDNHFEELLEAIEEIQGLTAL
                     LKALPSISDKREEYEGRLYAALTHLDHHVKPAIKEWDRVVDQMTDD"
     gene            complement(928..1161)
                     /locus_tag="VN647_11450"
     CDS             complement(928..1161)
                     /locus_tag="VN647_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97691.1"
                     /translation="MGTVCRNRDGMGRNKKLRVRIASLGQRITDHQIKIALERQRTTP
                     NVSLIRHWTVEIKAWKQTVAQLTRRLKKGKRHD"
     gene            complement(1234..1689)
                     /locus_tag="VN647_11455"
     CDS             complement(1234..1689)
                     /locus_tag="VN647_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97692.1"
                     /translation="MKNHSAVGLLLVLVFTAGTAQAEGFFDRMLDRTVESAERKARDR
                     ANQRIDQSIDKAINKTEETVQCVATDQECLKRAEDAGKQVSIVNHPTLSDSAKCVVTD
                     TACLKQAKAQGKKVEIVEEAELDTLRCSVADAECLKRAKAMGKKVEITD"
     gene            complement(1938..2700)
                     /locus_tag="VN647_11460"
                     /pseudo
     CDS             complement(1938..2700)
                     /locus_tag="VN647_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          729 a          962 c          907 g          861 t
ORIGIN      
        1 cccacgacga gacgcatgcc cttgccctca cactccaaac ggccaagcct ctggttactc
       61 atggcgctgc cggcatcagt cgaaaaggga aagatgtggg tcaagcttct cactactatt
      121 cgttcacgag gcttgagacg agcgggaagc tcacgatcga cggtgagtca ttcgatgtga
      181 ccggcaccag ctggatggac catgaattcg gatcgaccga actaggagcg gatcaagtcg
      241 gatgggattg gttcagtatc cagttagagg acgataccga actcatgctg tatcggatgc
      301 gtcggaaaga tggatcgtcc gatcttgcgt cgagcggaac tgccgtgtcc ccggatggac
      361 gcactcgaca cctggaggtg acggactttc aaatcgaatc gaccggaacc tggaccagtc
      421 ccgaaagcaa ggccacgtac cccagcaaat ggcggctgaa gttcccgtcg ctcgatttgg
      481 tgctggacgt cactccgttg gtggcggatc aggaattgcg cacctcgcgc agcaccaagg
      541 tctcctattg ggaaggagcg gtggcggtga cggggaccaa acaaggccgg cctatgaaag
      601 gccatgggta tgtcgagctg accggctatg ccgaacgcct caagatgtag gggaagaacg
      661 ggttagtcgt ccgtcatctg atccaccacg cgatcccatt ccttgatggc tggtttaacg
      721 tgatggtcaa gatgagttag ggctgcatac agccggcctt catattcctc tcgtttatca
      781 ctgattgaag ggagagcttt caacagtgcg gtcaatcctt gaatttcttc gattgcctca
      841 agcagttcct cgaagtgatt gtccaagagc gcactcgacg gcacaagcag cgtcccagac
      901 tcagggagtt ttgtccgccc tcgtttttta atcatggcgc ttccctttct tcaaccgccg
      961 cgtcaattgc gcaacggtct gtttccacgc cttaatctcg actgtccaat gccggatgag
     1021 gctgacattt ggagttgtgc gctgacgctc gagcgcgatt ttaatctgat gatcggtgat
     1081 ccgctgtcct aagctcgcaa tccgaactcg tagcttctta ttgcgcccca taccgtcccg
     1141 attccgacaa accgtaccca tgcttcggta tagctgtcaa ctggctcagt ataagtaaga
     1201 gggaaggggt gaggacgagg actatcgaac ccgtcagtcg gtgatttcga ctttctttcc
     1261 catggccttt gcgcgcttaa ggcattcggc atcggccacc gagcaacgca gtgtgtcaag
     1321 ttcagcttct tcaacgatct caaccttctt tccttgcgcc ttcgcctgtt taagacaggc
     1381 ggtatccgta acaacgcact tcgccgaatc ggatagggta ggatggttga caatcgagac
     1441 ctgcttgcct gcgtcctcgg cccgcttcag gcactcctga tcggtggcga cgcactgcac
     1501 ggtctcttcc gttttattga tcgccttgtc gatggattgg tcgatgcgct ggttcgcccg
     1561 gtcgcgagcc ttacgttcgg cgctttccac cgtgcggtca agcatgcggt caaagaaacc
     1621 ttcggcctga gctgttcccg ccgtgaacac taggacaagc aaaagaccga ccgcggaatg
     1681 gtttttcatc gcaacgcctc cttagggata cgccggtcgc aggactctag cacagcggaa
     1741 aggacagacc aagaggaagt cggattgaag catgccgtcg gtggtccatc catagtatct
     1801 cggtaaattc gacatggcgt agctgtgtga tgaggcttga gttaagcaca aagcaagatc
     1861 cgacccacag ttcacgcctt cgaccactga cctcaattcc tctgtatatc gatgaagtct
     1921 tgaattaggg cgtgtggtca attaagccag atggtagatg ctgcgagata gattgcgccg
     1981 aggaaggctg atgcccgttt atcgtatctt gtggcgatac ctctgaactg tttgagtttg
     2041 gcgaagaaat tttcgatcag gtgcctgatt ctgtacaggt cttcatcgta ttcccgctgt
     2101 tggatgcggg tggattttgg tgggatgacg gccagaactc cggctgtttc cagccaatcg
     2161 agaacgcgct cccgcgcatc gtaggctttg tcagccaaca gggcctgaat atcggtgaga
     2221 agcgatggcc gtaacgcgtc agccccgtgc agatcgtgca cctgtcccgg cgtgaggtgg
     2281 aagccggtcg gattgcccaa cgcatcgcag gtcgcatgga ttttggtggt caacccgcct
     2341 ttgctgcgtc cgatcgcttc ctgttcgcga tccccccttt tgcgcccgcg ctgtgctggt
     2401 gcgccctgac gatggtggag tcgatcatgg catattcgtt gtcggcgtct ttggccagat
     2461 gctcgaatac ctgttgccag acgccgctat ccgaccatct tttatggcga cgggcgtatt
     2521 gttccagtcc ccaaagcgct ccggcacatc gcgccatgga atgcctgcgc ggtaccggta
     2581 tatcacggct tcgacgaaca ggcggttatc cttcgctgtg acaccgaccg tctcctcgcg
     2641 ccccggcaat aaaccttcga tcctctccca ttgatcgtcg cgcagcccat atcgtcgcac
     2701 cattcataag cccctccaac ggcttataaa cagtgaatca caactcgatt cctgtgggaa
     2761 tcccaacaat caaattaatt gtccacacgc cctagacaga tcaatctatc catgtcctgt
     2821 tcaatctctc cttcaatttg ggatgcatcg gctctgagca agcctgttcc atcacaaggg
     2881 ctgcagtagc ctcgtaatac tctttgtctg ttccgtccca cccaattttg ttccaacata
     2941 cagacaggtt actggctgct cgcttgccca atagtctggt aatggcgcta gccacgtcac
     3001 gcattcttgt gatgccgccc cagacatgta actcttgtcc aatctcgcgt gctctctgat
     3061 gaaggttatg ctggagtgga ctgtcttttt gaaagaaacc cgaagacgcc ttgtcgggag
     3121 gaagcgtcca atctacgcct atgctgcaga ggtcagcgac atagcatcta aagcgtgtca
     3181 attcgtcctt cgcttgggaa gccttgccaa taaaatcatt tcctcctagg tgttgccata
     3241 acagttgtgc agcttgtggt tggtcatatt ccatggcata aaccaaaggg atctgccctt
     3301 ctctattctc aacttcaact agcagtttgt tgacaaactg cggtttttaa agaattcctg
     3361 catcgaattc accagttgat tctccgatga tggtcttgtg tgtcgatcat ggggttcgtc
     3421 tcgtcctcat cccgctcttt cgagttctcg ctccccgca
//
LOCUS       DATNDD010000474         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2241428, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000474 DATNDD010000000
VERSION     DATNDD010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241428"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VN647_11465"
     CDS             <1..401
                     /locus_tag="VN647_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97693.1"
                     /translation="VFREQLALKVLRILGHHYLKGDRPLKLPELSSELNMPLSLVEEE
                     VERLIENGFVGRLQEPQGVSLIKSPDLIFVKEVLDSVHNGTPPWVLPHLGTNDPVSAL
                     LHRRDKAVERVLAGETVQSLLQDQPPSQSV"
     gene            complement(575..1081)
                     /locus_tag="VN647_11470"
     CDS             complement(575..1081)
                     /locus_tag="VN647_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HWG97694.1"
                     /translation="MSAFVAMTAVMCSGIAVQTALGGEPTGPAVGYDIHVQAPHMMPD
                     GTSGGPYHHYCKGISDKILQCLLFESTDPKAPLVGIEYFVAKDLTRKLPAIQWHRHFH
                     DHKVEIATGRVQVLDMPPDQAAKVAEAAAGTDGVIYHLWQHGQEFPDGTVSFPQSLGH
                     KFPGYSDK"
     gene            complement(1252..2016)
                     /gene="ricT"
                     /locus_tag="VN647_11475"
     CDS             complement(1252..2016)
                     /gene="ricT"
                     /locus_tag="VN647_11475"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HWG97695.1"
                     /translation="MEPTLTEEFSHAYPESVRLVGVKVRNRGEVKKLDSVGVPLRTGD
                     PVLIEVEGEVTYGIVYLEPYSTPFIPPMRAMKSILRKPNTEETALIARLERLSLEAAT
                     YCRTKAMELGIHLKLVEVYGAMDHRQLTFVYTADDRIDFRELVRDLARRFGGRIEMRQ
                     VGVREEARRIGGIDSCGLVLCCASFLTDVKPISAKQAKKLDLPTDDPRLLGVCGRLKC
                     CLMFEMMDAQGTMAPQAQQLITPTRSRPPSSSSLLS"
BASE COUNT          550 a          602 c          621 g          535 t
ORIGIN      
        1 acgtgtttcg ggagcaactg gctctcaaag tattgcgaat tttggggcat cactacctca
       61 agggcgatcg tccattaaag ttgccggagc tatcgagcga actgaacatg ccgttgtctc
      121 tggtggaaga agaagtggaa cgcctcattg aaaacggatt tgtcggccga cttcaggagc
      181 cgcaaggggt gagtctcatt aagtcaccgg acttgatctt tgtgaaagag gttctggatt
      241 cggttcacaa cggaaccccg ccgtgggtcc tgcctcacct cggtaccaat gatccggtgt
      301 ctgccctctt acaccgtcgg gataaggcgg tggaacgtgt gttggccggt gaaaccgtcc
      361 aatcgctgct ccaagaccag ccgccatccc aatccgtctg attgccttta cggttgccct
      421 tctacttgcg gcttgttccg gttcggatca aacgatcgca cgtaagcaat acctgccagt
      481 tcttttcatg ggaccatctt gtggtcagag tggaccacca caatggtcct ctccggtctt
      541 gtgaggagag gacccgacca gtgtccttct caacttactt gtcggaatac cctggaaact
      601 tatgtccgag ggattgcggg aagctcacgg tgccgtcggg gaattcctgc ccatgttgcc
      661 agagatgata gatcacacca tctgtccctg ctgcggcttc cgctaccttg gcagcctggt
      721 ccgggggcat atccaagact tgaactcgcc ccgtcgctat ttctacctta tgatcatgga
      781 aatggcggtg ccactgaatg gcgggaagct tccgggtgag gtctttggcg acgaaatact
      841 cgatcccgac caagggtgct ttgggatcgg tcgattcgaa gagcagacat tgaagaatct
      901 tgtcggaaat acctttgcag taatgatggt aggggccgcc cgatgtcccg tccggcatca
      961 tgtgaggcgc ttgtacatga atgtcgtatc cgacggctgg acctgtcggt tctccaccga
     1021 gcgccgtttg aacggcaatt ccactgcaca tgaccgcagt catcgctaca aatgctgaca
     1081 agctcttgct cattgcaaca aacctcctag ctctgaggag ttttccccta gcccatctag
     1141 gtagaaaaca ccgccacgtt cgattgacca ttagagatgg ctcagtgaca aaggattcga
     1201 atatttcccg gagaaaatcc aacaacgacg taaaactgcg cggtgtatga tctaagagag
     1261 cagggatgaa gaggacggag gacgggatct agtcggtgta atgagttgtt gagcttgagg
     1321 cgccatggtc ccttgagcgt ccatcatttc aaacatcaaa cagcacttca gccttccgca
     1381 gacgcccagc aagcgaggat catcggtcgg caggtcgagc ttttttgcct gcttggccga
     1441 gataggtttc acatcggtca gaaaactcgc acagcacagc acgaggccgc aggaatcgat
     1501 cccgccaatc ctccttgcct cttcgcgcac cccgacttga cgcatttcga tgcggccacc
     1561 gaaacgacgt gccaggtctc ggaccagttc tcgaaaatcg atccggtcat cagccgtgta
     1621 aacaaaggtg agttgtcgat gatccatggc cccatagacc tcaaccagct tgagatggat
     1681 gccaagttcc atcgctttcg ttcggcaata ggtggcagct tccagggaga gccgctcaag
     1741 cctggcgatt aacgcagttt cttcagtatt aggcttgcgc aatattgact tcatggcccg
     1801 catgggcgga ataaagggcg ttgagtaggg ttctaggtaa acgatcccat acgtgacttc
     1861 accttccact tcgatcaaga ccgggtcacc ggtgcgcaat ggcacaccga ctgagtccaa
     1921 tttcttgacc tctccccggt ttctgacctt cactccaact aggcgaaccg actcgggata
     1981 agcatgggag aactcttcgg taagggtagg ttccataggt cgatcataca caaatttatg
     2041 caatagggga acagttgcta aagggacaac gatcgaagta cccgaatctg ttgcaagaga
     2101 cgagaaaccc ataaggcatg gataaaacat gagaaaatgg tcaacactcg acctgccttg
     2161 ttatggtagg ctaagtcagc ggtgggagga agatggggtg aacgatgggg acaatggtca
     2221 gagaacgaaa aaagatgtta cgcattccca atcaggttgt ccttccgttc ggctaccgga
     2281 tttgggttcg acagttatcc gatgcgga
//
LOCUS       DATNDD010000475         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_811835, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000475 DATNDD010000000
VERSION     DATNDD010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811835"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VN647_11480"
     CDS             <1..1635
                     /locus_tag="VN647_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HWG97696.1"
                     /translation="ARPASGQDSGAPDEPDPLKRKRSDDAEFKAQKELRKELKGPFKT
                     WLDQDVAYIITDEERKAFKSLSNDEEREAFIEQFWLRRNPNPDSPDNEFREEHYRRIQ
                     YANDHYAAGKPGWKTDRGHIYISFGAPDSIDSHPSGGSYNRPLDEGGGETSTFPFEVW
                     HYRYLEGVGDNIDIEFVDTCQCGDYHFTIDRGEKDALAKVPGAGLTQWESIGMAKKAD
                     RFKGGFESLGTGPLGSSNESKQFDRIEMAAKLFAPPPIKFHDLESAISEHKIMQGPVF
                     PFDVRTDLVKVTSSTVLVPITVQIKNRDVTFVTKDGVATGVVNILGKVTTLTHKTVQT
                     FEDTVSVQQPAELLQASLDRKSIYWKAVPLLPGLYRLDIAIKDVNNPDHIGVYGRSLE
                     VPQYHDEKLGTSSLILADRIYPVSSHDIGQGNCVIGNIHVCPRVTPNAATPVNYKRNQ
                     ELNFWMQVYNLGINDQTKANEAKVTYQIIDTATNTVVFEKEQDSKDFGEHSDQLTVEK
                     TLPVAGLQPGKYKVTVKVNDEISKQEIAQSAPFVVE"
     gene            1748..>2033
                     /locus_tag="VN647_11485"
     CDS             1748..>2033
                     /locus_tag="VN647_11485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWG97697.1"
                     /translation="MIRRLHLVSLIALIAASGVSGFGASVGGAPNGTVSGIVRDSAGV
                     PQLGAAVELLRPDLTVVAAVYTNNKGLYTFSKVLPGHYAVKAMGTSFLPSL"
BASE COUNT          504 a          511 c          595 g          423 t
ORIGIN      
        1 gcccggccag catccggtca ggatagcggt gctccggatg aaccggatcc gctgaagcgg
       61 aagcgttcag acgatgccga gttcaaagcg cagaaggaac tccggaaaga gttgaaaggg
      121 ccgttcaaga cctggctcga ccaggacgtg gcgtacatca ttacggacga ggagcggaag
      181 gcgttcaaaa gtctgagcaa cgacgaggag cgcgaggctt tcatcgaaca gttctggctg
      241 cggcgcaatc cgaatccgga ctcaccggat aatgagttcc gggaagagca ctacagacgc
      301 atccagtacg ctaacgatca ctatgcagcc ggcaagccgg gttggaagac agatcgcggt
      361 catatttaca tttcatttgg agccccagat tcgatcgatt cgcatccatc cggcgggtct
      421 tacaatcggc ctctggacga aggcggaggc gagacatcga cgtttccgtt tgaggtgtgg
      481 cactaccggt atctcgaggg cgtgggcgac aacatcgata ttgagtttgt ggatacgtgc
      541 cagtgtggcg attaccactt cacgattgat cgtggcgaaa aggacgcatt ggcgaaggtt
      601 cccggagccg gcctgacgca gtgggagtca attgggatgg cgaaaaaggc ggatcggttc
      661 aaaggtggct ttgaaagcct cggaacaggg ccgctgggga gttccaacga atcgaagcag
      721 ttcgatcgca tcgagatggc agccaaactg tttgcacctc caccgatcaa attccacgac
      781 ttggaaagtg caatcagcga acacaagatc atgcaagggc cggtattccc gttcgatgtt
      841 cggacagatc tggtgaaggt gacatccagt actgttctgg ttccgatcac ggtgcagatc
      901 aagaatcgcg atgtcacatt tgtgaccaag gacggggtgg cgaccggtgt ggtgaatatc
      961 ctgggcaagg tgaccacgct cacacataag accgtccaga cctttgaaga tacggtctca
     1021 gtgcagcaac ccgcagagct tctgcaggca tctttggatc gtaagtctat ctattggaaa
     1081 gccgtaccgc ttctccccgg tctctatcgc cttgatatag caatcaagga tgtgaacaat
     1141 ccggaccata tcggcgtcta cggtagaagt cttgaagttc cgcagtacca cgatgaaaag
     1201 cttggtacat cttccctcat tttggcggac cggatctatc ccgtgagctc tcacgatatc
     1261 gggcagggca attgcgtaat tggaaacata catgtttgcc cgcgggtgac gcctaatgca
     1321 gctactccgg tgaactataa acgtaaccag gagttgaact tctggatgca ggtttacaac
     1381 ctgggcatca atgatcagac caaggcgaac gaagcgaagg tcacgtatca gatcatcgac
     1441 acagcgacga acaccgtcgt attcgagaaa gagcaggatt caaaggactt cggcgaacac
     1501 agtgatcagc tcacggtgga aaagacactg ccggtagctg ggctgcaacc cggcaagtac
     1561 aaggtgacgg tgaaagtaaa tgacgaaatc tcaaaacaag agattgcgca atcggcacct
     1621 tttgtcgtgg aatagaacga agggctccgc aaggtaggtt aaccggcagt agatcgggaa
     1681 cctgtgagtc tcgagtggtc aattcccagg gctttgccag gacgccgccg ggtcaccaac
     1741 gcaagtgatg attcgcaggc tccatctcgt ctccctgatc gcgctgatcg cagcatctgg
     1801 cgtgtccggc tttggtgcgt ccgtgggagg cgcgccgaat gggaccgtct caggcattgt
     1861 gcgtgattca gcaggcgtcc cgcaactggg tgcagcggtg gagttgctga ggcccgatct
     1921 gactgtggtt gccgcagttt atacaaacaa caaaggcctc tataccttct caaaagtgtt
     1981 gcccggtcac tatgcagtga aggcgatggg tacctcattt ctgccatcgt tgc
//
LOCUS       DATNDD010000476         5141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3109297, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000476 DATNDD010000000
VERSION     DATNDD010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109297"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VN647_11490"
     CDS             complement(<1..1273)
                     /locus_tag="VN647_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWG97698.1"
                     /translation="MRRAAGWTTTALGFTIPLWVVADGILLGLLVLCWTVSGGWRERL
                     RRISANPVAVAAIVLFAWLLLGTVWGGGTVEERAFWVKKYANLLLVPLLISMVIDARE
                     KNRALLALAASLAVTLALSFALSMGILPTGGLINCDPFNPCLLKKQIPCDPSIPCVPA
                     NYPPVCDPSNPCILKKHTTHNLLMAFGVLLFGILAWKSSRRWARWGWAGAACLAVGNV
                     LLMVQGRTGYVVLAGLAMVAFHMYFGWRGMVAAVIALSVTFSAAYQISTSFHDRVNLV
                     ATGVAQWSSQVAAEDPIGQRLEFYYHTVQVIRDHPLMGAGTGGFAQAYAVYAKQAGVP
                     VPSHPHNQYLLIMAQVGIIGLILLLWLFVQQWRWTSLTGDATYGLLAKGLVVTIAISC
                     LFQPALNDHTEKLFYCWFSGLMYSGIDSQAGT"
     gene            complement(1306..1992)
                     /locus_tag="VN647_11495"
     CDS             complement(1306..1992)
                     /locus_tag="VN647_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWG97699.1"
                     /translation="MEDLFKGSIDRFCEIAIRLSEAKRVLDVGSGQGLLLSFLAELGH
                     KCHALDVKDQPSIYPDTYGKGITFRLCNVEADAIPYPDESFDAVVCCQVLEHFSHSHL
                     PAVQEMKRVLLPGGVLEIDVPNVASFRNRLRLLRGKHITYDYAEHYLHAAPTLYKGMS
                     YYPLRHNREFTRGELELLLRSAGFQRYEVSFLRSRRYREGIQRARSVGTAMKDMIPSL
                     RKSLIAFAYK"
     gene            complement(2102..3262)
                     /locus_tag="VN647_11500"
     CDS             complement(2102..3262)
                     /locus_tag="VN647_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWG97700.1"
                     /translation="MSIMMVILPGIIAFVSAWWLTNRLCSPKSFLSILAHPNERTLHA
                     MPTPQTGGLAVIGSVVISLILAASVLAITQPSKPVLPKGVTSGSVWIVVSMLLIFVVS
                     FIDDCVGLPAALRLGVQAVSACIIIGGVGLTLSFIPIPGGPHILLGIAAIPVSVLVLL
                     WMANLYNFMDGMDGFAGGMTFFGFGFLAYFGWQAHFPVMLIIATFVAMGALGFLTHNF
                     PPARIFMGDAGSITIGFLAGTLMILGVRDGIFELWVPVMIFSPFIVDATVTLIRRVLR
                     RKKIWQAHREHYYQRLVLSGWSHRRTVLAEYGVMILSGGLAVLYHHSTDDVRLIILGA
                     WVGMFFLLGSLVGKLERNRKVACSLRDPAASGKNAEVPVVSVQDPPRVVVKT"
     gene            complement(3449..4444)
                     /locus_tag="VN647_11505"
     CDS             complement(3449..4444)
                     /locus_tag="VN647_11505"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWG97701.1"
                     /translation="MTKVLVTGAAGFLGSSLVEELCRSGYQVRAVVHDSRLVTSCSRE
                     VEIVVADICDFKKIREITAGCGAIVHLAAKVHAIDDSGAEKDYEAVNVEGTRHILNAA
                     VASGVGHVVFASSVKVFGEETKGCIDESQTPDPQTPYGRSKWKAEQLVSEYAGRHGLI
                     AVSLRLPMVYGPTRKGNLYRMIEAIDHGRFPILPRLSAVRSLLHVQNFIQAVLLCLRA
                     PCFKQGAYIVADTEPYCVTDLYDWLRAGLGNAPPRWRVPLWILKGGARCGDVFHAISG
                     RRGPLTTEQLTKLIGCAWYSSAAITRELDYRPTYSLEKAVPELIAFYRDETHAGC"
     gene            complement(4441..>5141)
                     /locus_tag="VN647_11510"
     CDS             complement(4441..>5141)
                     /locus_tag="VN647_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWG97702.1"
                     /translation="HDDQDRLVGEGVVPVSRTRIIAGSGIDIAVYSLQLQPSGVPIVV
                     LPARMLWDKGVGEFVEAARRLKRKGAHARFVLVGRRDEHNPAAIPEIRLKEWVKEGVV
                     EWWGHREDMPAVYAAAMLVVLPSYREGLPKVLLEAAACGKAMVATDVPGCREIVRDRF
                     NGLLVSPRDSMALANAMEGLLSDQKSREIMGQRSRTLALNEWSGSRISEQVLNLYHDV
                     LKSSSIADRSHGYA"
BASE COUNT         1322 a         1512 c         1278 g         1029 t
ORIGIN      
        1 tcgtaccggc ttgcgaatca atccccgaat acatcaggcc ggagaaccag caataaaaca
       61 gtttttcagt atggtcgttg agcgctggtt ggaacagaca gctgatcgcg atggtgacta
      121 ccaatccctt cgcaagcaac ccatatgtcg catcgccagt gagcgatgtc catcgccatt
      181 gttgcacaaa gagccaaagc aataggataa gcccgattat tccgacctgt gccatgatca
      241 agaggtattg attgtgcgga tgcgagggga caggcacgcc ggcttgtttt gcatataccg
      301 cgtacgcttg agcaaatcct cccgtcccag ctcccatcag cggatggtcc cgaatgacct
      361 gaaccgtatg atagtaaaat tccaatcgtt ggcctatcgg atcttcggct gccacttggg
      421 agctccattg tgcaacacca gtggcgacaa gattgacccg atcatggaaa gaagtggaga
      481 tttgataggc cgccgaaaat gtgacactga gggcaatgac cgctgccacc atgcctcgcc
      541 acccgaaata catatggaag gccaccatgg caagcccggc cagcaccacg tagccggttc
      601 gaccttgaac catcaataag acattgccca cggccagaca tgcagctccc gcccatcccc
      661 atcgtgccca tcggcgtgag gacttccagg ccaaaatccc gaacaacagc acaccaaatg
      721 ccatgagcaa gttgtgagta gtgtgttttt tcaatataca cggattagat ggatcacaga
      781 cgggagggta attggcgggg acacagggaa tggatggatc acatggaatc tgctttttca
      841 ataaacacgg attgaaagga tcacaattga tgagtccgcc ggttggaagg atccccatgc
      901 tcaaggcaaa agacagtgcc aatgtcacgg ccagcgatgc cgccaaggct aggagcgcgc
      961 ggtttttttc tcgggcatcg atcaccatcg aaatcagcag ggggacgagc aacagattag
     1021 cgtatttctt cacccagaac gctctctctt ccacagttcc ccctccccag accgtaccta
     1081 gcaatagcca agcgaacaat acgatagccg caactgccac tggatttgcg ctgatccgac
     1141 gaagtctttc acgccacccg ccactgactg tccaacatag cacgagcagg ccgagtagaa
     1201 tgccgtccgc gacaacccag agcggaatgg tgaatccaag cgcagtcgtg gtccagcctg
     1261 ccgctcgtct cacctccgcc tgcagcctgt ctctcgtcca acccatcact tgtaggcgaa
     1321 ggcgatcaag gatttccgaa gcgacggaat catgtctttc atcgcggtac ccaccgacct
     1381 cgcccgctga atcccttcgc gataacgccg ggatctcagg aaggacacct catatctttg
     1441 gaaccccgca gaacgcaaca gcaactccaa ctcaccgcgg gtaaactcgc gattgtgtcg
     1501 aaggggataa tacgacatgc ccttgtacag ggttggcgcc gcatgaagat agtgctcggc
     1561 ataatcatag gtaatgtgtt tcccacgcaa cagacgaagc ctgttacgaa aactggcgac
     1621 gttcggcaca tcgatctcca gcacacctcc gggcaacagt actcgcttca tctcctggac
     1681 cgctgggaga tgcgagtgcg aaaagtgttc gagcacctgg caacagacga ccgcatcgaa
     1741 cgattcatct ggatagggaa tcgcatcggc ttccacatta caaagccgaa acgtaatgcc
     1801 tttcccatac gtatcagggt aaatggaagg ctgatccttc acatcgagcg catgacactt
     1861 atggcccagt tcagccagaa acgagagcaa taagccttgg ccggacccga catcgagcac
     1921 ccgcttggct tcactcagcc ggatcgcgat ttcgcagaac cgatcgatgc ttcccttgaa
     1981 caaatcctcc atttccgggt cgcctcgtcg aaaaagacgg ccttcattcc gcaaccggac
     2041 ggcggaatgc aaaaggcccc tgctgtttgt cgatcggccc acggcaagat cggatttcag
     2101 gctatgtctt aacgacgacg cgtggagggt cttgaaccga cacaacaggc acttcagcgt
     2161 tcttgcctga tgcagcagga tctcgaaggg aacaagcaac ctttcgattc cgttccaact
     2221 tgcccaccaa actcccgagc aggaaaaaca tgcccaccca tgcaccaaga ataatgagtc
     2281 tcacatcgtc cgtcgaatga tggtacagaa cggccagccc tccggacaga atcatgaccc
     2341 catattctgc aagcaccgtc cggcgatggc tccatccgct caagaccaac cgctgataat
     2401 aatgctcccg atgggcttgc cagatctttt tgcgacggag cacccgtcgg atcagggtca
     2461 ccgtcgcgtc cacaatgaat ggtgagaaaa tcatgaccgg gacccacaac tcgaatattc
     2521 cgtcgcgaac cccaaggatc atcaatgtcc ctgccaagaa tccaatcgta atgctccccg
     2581 catcgcccat gaagatacgt gcaggcggga aattatgggt gagaaatccc agcgcaccca
     2641 tcgcaacgaa cgtggcgatg atgagcatga ccgggaaatg ggcctgccac ccaaaatagg
     2701 cgagaaaacc aaaaccaaaa aacgtcatgc cgcccgcaaa accatccata ccgtccataa
     2761 agttatagag gttcgccatc cagaggagga caaggacact caccggaatt gcggcaatcc
     2821 caagcaggat atgcgggcct ccgggtattg ggatgaatga caaggtcaag ccgacacccc
     2881 cgataatgat acaagcggag actgcttgga cacccaatcg gagagctgcg gggagaccga
     2941 cacagtcatc gatgaaggac acgacaaaga tgaggagcat cgaaaccacg atccagacgc
     3001 tccctgacgt cacacctttc ggcaatacag gtttcgaggg ttgggtaatc gccaacacac
     3061 tggcagccag aatgagactg atcaccacac tgccgatgac ggccaaccct ccggtctggg
     3121 gagtcggcat agcatgcaat gttcgttcgt tgggatgagc aagtatcgag aggaacgatt
     3181 tcggcgaaca gagacggttt gtgagccacc aggcggatac aaaggcaatg attcctggaa
     3241 ggatgaccat catgatcgac atatcgaatg ctaccttatt agatgccgca ctaacagccc
     3301 cgccctggac tcaagagatt ttgctgtttg ttcggcaagc tctatgactc atttttacat
     3361 tgaaatcaag acgtccgagc cttcgaaccc gtatggttgg aagtgttacg tctacgtgag
     3421 ccacatgaga gctcaactat gagatgtctc aacagcccgc atgtgtctca tcgcgataaa
     3481 atgcgatcag ctcgggcacc gctttctcta aagaatacgt aggccgataa tctaattcgc
     3541 gtgtgatagc ggcggaactg taccaggcgc agccgataag ctttgtcagc tgctccgtcg
     3601 tcaagggacc acgccttcca ctaatagcgt ggaacacatc accgcatcgc gcaccgcctt
     3661 tgagtatcca gaggggaact cgccatcgcg gaggtgcgtt tcccaatccc gctcgtaacc
     3721 agtcgtagag atcggtcacg caatacggct cggtatcggc tacgatgtat gcgccctgct
     3781 taaagcaggg cgcgcgaaga cacaggagta ctgcctgtat aaaattttgg acatgtaaga
     3841 ggcttcggac cgccgaaaga cgaggcaaga tcggaaacct cccatgatcg atggcctcaa
     3901 tcattcgata caagttgcct ttcctcgtcg gaccatagac catcggaaga cgaagtgaga
     3961 cagcaatgag gccatgtcgg cctgcatatt ccgagacaag ctgttcggcc ttccattttg
     4021 atcgaccata gggggtctgt ggatccggag tctgcgattc atcgatacac cctttcgttt
     4081 cttccccaaa gaccttgacc gaactcgcaa acaccacatg gcctacgccg gatgctaccg
     4141 ctgcattgag aatgtgcctc gtgccttcga cgttgacggc ctcatagtcc ttctctgctc
     4201 cggaatcatc gatggcatgg accttcgccg ccagatgcac gatggctcca cagccggccg
     4261 ttatctcccg gatcttcttg aagtcacaga tatccgcgac aacgatttct acttctctcg
     4321 aacacgaggt gaccaacctt gaatcatgaa cgacggctcg aacttgatac cctgaccgac
     4381 agagttcttc aaccagggac gacccaagaa accccgctgc tcctgttacc agcactttgg
     4441 tcatgcgtac ccatgcgagc gatccgctat agaagaagac ttcaatacat cgtggtacag
     4501 gttgaggact tgttcactga tccttgaacc agaccattca ttaagagcga gtgttcggct
     4561 tcgctgtccc ataatttccc gcgacttctg atcggacaac agtccttcca tggcgttcgc
     4621 caacgccatg gaatctctag gagaaaccaa caacccgtta aaccgatcac gcacaatctc
     4681 tcggcagccg ggaacatccg tggccaccat ggctttgccg caggccgccg cttccagcaa
     4741 cactttggga agtccctcgc gatacgaggg caacacgacg agcatggccg ctgcatagac
     4801 agccggcata tcctctcgat gtccccacca ctcgaccacc ccttccttca cccattcctt
     4861 caggcggatc tccgggatag ccgcggggtt atgttcatcc cgtcgcccga cgagaacgaa
     4921 ccgggcgtga gcgccttttc gtttcaaccg ccgggcggcc tcgacaaatt caccgacgcc
     4981 tttgtcccag agcatgcgtg caggcagaac gacaatcggg acaccggacg gctgcagctg
     5041 taaagaatac accgcgatat cgatgccgga acccgcgata attctcgtac gagagacagg
     5101 aaccaccccc tctccgacga gccggtcttg atcatcgtga t
//
LOCUS       DATNDD010000477         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3462090, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000477 DATNDD010000000
VERSION     DATNDD010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462090"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VN647_11515"
     CDS             complement(<1..406)
                     /locus_tag="VN647_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97703.1"
                     /translation="MKGLVKIAVVMGLYLIEGLIVTSVQAVDTEDVTTRVERSFDQLK
                     NYQEEKAADKSSSVRHTVEVGHGAYSSDQYWIGRGQGDLTKGKVVCQRVSELSARTDL
                     AKQIRVLVKEHMIDRVRERSGRESEQDIELTRE"
     gene            complement(403..1431)
                     /locus_tag="VN647_11520"
     CDS             complement(403..1431)
                     /locus_tag="VN647_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97704.1"
                     /translation="MSGRLFHQPPFPLFLPSHKSYRLIVIFLAVGAFMAAMAAYPVLA
                     EIRVVNAQGEHRMGNRDTREDAIRLATEAAKRNALEQGATYLESVTVVHDMDVTKDEI
                     RSYTAGLVLVLNQQIATTLDGDVVVVKVDLLAQMDTDEVARAIATLRENEDARVQLAA
                     LKQENEQLQEELKTVNQALGNAATAEQVQQAAQKRKEILNRVQSNAMVSQAWTNWVLV
                     SPVGFPQALLNSASGLSPANPHVAIAQQIIANRQPLGPRQPPAPGNIQPQMPRHELTP
                     PPGQSSVPRAPNEIIHRAPPGRIQSDHQPMTQPGQGSGRQQPRPHYRFDQSLGVPGGG
                     PRSGGGRP"
     gene            1607..>2032
                     /locus_tag="VN647_11525"
     CDS             1607..>2032
                     /locus_tag="VN647_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWG97705.1"
                     /translation="MTHPSRFFMWILVGLLTILLEIGAQAPVDAAPPTGKETAPSGSG
                     TEHVILFVLEGFGQKSLQGGTMPVLSKLVKEGSVTWSASGVKPALRLPTMASLVTGMP
                     VEKHGITWNFFEFSRGYPRAPSLFDYLDLSGGRDSAIFYM"
BASE COUNT          394 a          600 c          558 g          480 t
ORIGIN      
        1 cttcacgagt cagctcgata tcctgctcgc tctcgcgccc cgaccgttca cgaactcgat
       61 cgatcatgtg ttctttgacc aatacgcgaa tctgcttggc aagatcggtc cgagccgaga
      121 gctccgacac gcgctgacaa acgaccttgc ctttggttaa atctccctga cctctgccga
      181 tccaatactg gtctgaagaa tatgcgccat ggccgacttc aaccgtatgc ctgaccgatg
      241 aagacttgtc cgctgctttc tcttcctggt agttcttgag ctgatcgaac gatcgctcga
      301 cccgtgtcgt gacatcctcc gtgtcaaccg cctgcacact ggtgacaatc agcccctcga
      361 taaggtagag acccatcacg acggcgatct taaccaagcc cttcacggcc ttccacctcc
      421 ggatctagga cctccccccg gaacgcccaa ggattggtcg aaccgatagt gtgggcgtgg
      481 ctgctgccga ccagaacctt ggcccggttg ggtcatgggc tgatgatcgc tctgaatccg
      541 tcctggaggt gctctatgaa tgatctcatt cggcgcccgt ggaacgctcg actgcccagg
      601 aggcggagta agctcatgcc gtggcatctg aggttgtata tttccaggcg cgggcggctg
      661 ccgagggccc agcggctgtc ggttggcgat gatctgctgc gcaattgcga cgtgcgggtt
      721 ggccggagaa agcccactcg cactattcag caatgcctgg ggaaacccga ccggtgagac
      781 aagcacccaa ttcgtccagg cttgcgacac cattgcgttc gattgcacac gattgagaat
      841 ttccttccgc ttttgggccg cctgttgaac ctgctccgct gttgccgcat tgcccaaagc
      901 ctgattgacg gttttcagtt cctcttgcag ctgttcattt tcttgtttca gcgccgccag
      961 ctgaacacgg gcatcttcgt tttcgcgaag cgttgcgatc gcacgcgcca cttcgtccgt
     1021 atccatctgt gcgagaagat ccactttcac gaccacgaca tcgccgtcca acgttgtggc
     1081 gatctgttga ttgagcacga gcaccaatcc ggccgtatag gaccggattt cgtctttggt
     1141 gacatccata tcatgaacga ccgtgacgct ctcgagatag gtggcacctt gttcgagtgc
     1201 gttccgtttg gccgcctcgg tagcgagtct gatcgcgtct tcccgggtgt cgcggttccc
     1261 catgcgatgt tcgccctggg cattgacgac cctgatttca gcgagtacag ggtaagcggc
     1321 cattgcggcc atgaatgccc cgaccgcgag aaaaatgaca atgagtcgat acgatttatg
     1381 agacggcagg aacaagggga aaggtggttg atggaataat cgaccggaca tggcttcccc
     1441 ttctcacccc ctgcgctcgg atggaaacgg tcagcggttc cagcatagca cctggaaggt
     1501 ggttccgcca gcaatcggcg ggtgccttcc ccaccttgcc ttccggaaaa tttgcatgtt
     1561 agggtaccac tcctactcgt aggatacatg cgggtgatat tttgtcatga cacatccatc
     1621 acgatttttt atgtggatcc ttgtcggtct tctgaccatc ttgcttgaaa tcggggccca
     1681 ggcgcctgtc gatgcagctc ccccgacggg gaaggagacc gctccctccg gctctgggac
     1741 cgaacatgtc atcctctttg tcctggaggg cttcggtcaa aagtcgcttc agggcggtac
     1801 gatgccggtg ctgagcaagc tcgtcaagga aggatcggtg acctggtccg cgagcggggt
     1861 caaaccggcc ctgcgattgc ccaccatggc atcgctggtg accggcatgc cggtggagaa
     1921 acatggaatt acctggaatt tctttgaatt cagccgaggc tatcctcgcg ctcccagtct
     1981 attcgactat ctggatctaa gcggaggccg cgacagcgcc atcttttata tg
//
LOCUS       DATNDD010000478         4721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2826414, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000478 DATNDD010000000
VERSION     DATNDD010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2826414"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VN647_11530"
     CDS             <1..233
                     /locus_tag="VN647_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWG97706.1"
                     /translation="MGIDRNTTISGLDGSDAVRLWFQWRRGDTIARDTLLSYNRADTE
                     NLVSLADRFYEDLVSRFGPTSLSNTLQPAYSR"
     gene            230..985
                     /locus_tag="VN647_11535"
     CDS             230..985
                     /locus_tag="VN647_11535"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWG97707.1"
                     /translation="MSTWIGIGRSEVGLVRTLNQDAFAVIDSVSVWAVADGMGGHVGG
                     EVAAQTAIATVQAEAAASSHLLGNGQQAPTDVLTDLINRAHDAILNRARSKSKLKGMG
                     TTIVLLAIVSGPAPIAYLVHVGDSRAYRFRSGTLTLLTKDHTLIEKYLERGILTAESA
                     KTHPERHVLTRALGVGATVKPTITAFPLLPEDLVLLCSDGLTKMLEDEEIRTVFAAGE
                     LDPIQVCNRLVTAALDRGGEDNVTVVVVAPRSS"
     gene            1076..1954
                     /locus_tag="VN647_11540"
     CDS             1076..1954
                     /locus_tag="VN647_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWG97708.1"
                     /translation="MPRRGSFVCGSLFLLIGLTTVAGAGEPSDPEAAIRQMVRANAEK
                     DLPTLSRLMAHDADIVSYSVGGRKYVGWPEFEREMREEFINAQKIEIPITELKVWTKG
                     DLAWFTMELDYTRYVGEGSSVKRTVLPLRETGVLERRAGRWQLLSWHESFRSAQLGGP
                     LASPLMTGGSQKLVNNAAAAAMPDVSGEWDILEVEDEKRYKATLDKNGNGPYTQQGGR
                     FTTTKIENRLWQGTWHQPGNDREGGFEVLLSEDGTQAKGIWWYTRVDTRKNIPPKEHG
                     GTYHWKKVIPSPANAQ"
     gene            2035..3039
                     /locus_tag="VN647_11545"
     CDS             2035..3039
                     /locus_tag="VN647_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97709.1"
                     /translation="MRRSLILFISTFFMISSTYADGGHDHHAIPTLKKQVGSKIAYGE
                     NTATLTFGPIDLPTGHGEGDMGDSMPRFNFQLPEDKYLVGFKSALSTVDGKELPRNYL
                     HHILMINNTKPSVSCPGEPLFFGGAGLEMSETQFPEGYGVKLSATDKLMTVVAFYHGA
                     PPTKDVIATFTMYFAPKVKPVKEMEIYQVGVNIVCFTKFGDRPADQTDEGIEIGPGVQ
                     VRTAPLKFSMDGCVKYAYPHGHDELLLIALENKTKRQTLLRTVPDVEPDGTLREFLPH
                     QVYKDVQGFPISKEDDYEMVMVYHHSLQRTELQHGMGNYLLYMTPGACSGQGKTAAAY
                     "
     gene            3328..>4721
                     /locus_tag="VN647_11550"
     CDS             3328..>4721
                     /locus_tag="VN647_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWG97710.1"
                     /translation="MPQSEPVAILLVNAIAEEIKLATLNFRRFFPNCRVEAVYTVEEA
                     LQWGQRASWQLILIDEDLLAQRAAPVFSELRRLVPYATLVLQTAHSDSASSLNALQAG
                     ADFLLYKQSPAFLAELMLYTKSALETRAIRMTLKQTEDRHGCLIDILSDGLYELDGEG
                     RFVYLSPLATEMLGYTENELIGTLYSTVVPPDQQDRARHRFNDRRTGTRAARRIEIDL
                     IRKTSSDKPAHTRVRAEISARGLYDFNRRHLGTLGLLRDISHHRRQTKTIDQLEHQLR
                     ESDRLVAIARRFSTLSKNLHAPHNAIQTQSQLLLKTIRDARLIEQVDSLATYAAEAAH
                     LGQELVQTTTEMVTRPDTINDIIETILAAATPPPTLANIEWIERAYEQNLPSFTGSLD
                     SMMDLMRILLTHARRYVVAVGTPHRLRISTRAINEAGDPVAQETSLLPSTAAAEFEIH
                     IQETDIIISAEEPPR"
BASE COUNT         1091 a         1403 c         1236 g          991 t
ORIGIN      
        1 aaatggggat tgaccgcaac acaaccatca gcggcctcga cggatcggac gcggttcgcc
       61 tgtggttcca gtggcgccga ggtgacacga tcgcccgcga taccctcttg agttacaacc
      121 gggcggatac cgaaaacctc gtgtcccttg ccgaccgctt ctatgaggac ttggtctccc
      181 ggttcgggcc tacctcattg tcgaacactc ttcaaccggc ctattcccga tgagcacctg
      241 gatcggcatc ggtcggagcg aggtcggact cgtacgtacc ctcaaccagg atgcgttcgc
      301 tgtcatcgac tcggtgagtg tctgggccgt cgcggatgga atgggtggtc atgtcggtgg
      361 cgaagtcgcc gctcagacag ccatcgccac ggtacaggcg gaggctgcag catcgagcca
      421 cctgcttggc aacggccagc aggctcccac ggatgtgttg acggacctga tcaaccgagc
      481 gcatgacgcc atcctcaatc gggctcgatc aaagtctaag ctgaaaggga tgggcacgac
      541 catcgtgctg ctggcgatcg tctccggtcc ggccccgatc gcttacctcg tccatgtcgg
      601 cgacagtcgt gcctatcggt ttcgatcagg cacattgacc ctcctgacca aagaccatac
      661 gttgatcgaa aaatacctgg aacgcggcat tctcacggca gaatcagcaa aaactcatcc
      721 ggaacggcat gtgttgacac gtgccttggg ggtaggcgcc acggtgaaac ctaccattac
      781 ggcctttccc ctgctgccgg aggatctggt cctgctctgc tcggatgggc tgaccaagat
      841 gttggaggac gaggagatcc gaaccgtgtt cgctgccggc gagctcgacc cgattcaagt
      901 gtgcaaccgc ctcgttaccg cggcactcga tcgtggcggc gaagacaatg tcactgtggt
      961 agtggtcgcc cctcgttcgt cctgatttta cttcgttgtt gacatggccc gtcaggccgt
     1021 gtagtttccc ttctcatttg aagctttgaa ccttgtcccg tacatggaga cccttatgcc
     1081 ccgtcgggga tcgttcgtct gcggaagtct gttccttctc atcggcctta cgacggttgc
     1141 gggcgccggt gaaccgtccg acccggaagc ggccattcgt caaatggtcc gggccaatgc
     1201 ggaaaaagat cttccgacgc tatctcgtct catggctcac gatgccgata tcgtgagtta
     1261 ctcggtcggt ggacgaaaat acgtcggttg gccggagttc gagcgcgaaa tgcgtgagga
     1321 attcatcaac gcccagaaaa ttgagatccc cattacagag ctcaaagtct ggacgaaagg
     1381 agacctggcc tggttcacga tggagctcga ctatacccgc tacgtcggtg aaggatcctc
     1441 agtaaagcga acagtacttc ctttaagaga aaccggtgtc ctcgaacgtc gtgccggccg
     1501 ttggcaacta ttgtcgtggc acgagtcgtt tcgatccgcg caactaggag gtcctcttgc
     1561 ctcaccattg atgacgggcg gatcgcaaaa gctcgtcaac aatgctgctg cagcggcaat
     1621 gcctgacgtg agcggagaat gggatattct cgaagtcgag gacgaaaaac gatacaaggc
     1681 aacgttagac aagaatggga acggccccta cacgcaacag ggcggacggt ttactacaac
     1741 aaaaattgag aaccgactgt ggcaaggcac ctggcaccag ccaggcaacg atcgcgaagg
     1801 cggatttgag gttctgctct cggaagacgg cacgcaagca aagggaatct ggtggtacac
     1861 acgagtcgat acgagaaaaa atattccgcc gaaggaacac ggcggtacct accactggaa
     1921 gaaagtgata ccctcaccag ccaacgccca atgagtcagt ttgtttcttc tcagccatac
     1981 caacaactcg gagcccggtt caccatcact gcttcacttc ctctgggagg tcttatgcgc
     2041 agatccctca ttctttttat ttctaccttc ttcatgatct catcgaccta tgccgatggg
     2101 ggccatgatc atcatgccat ccccaccctg aaaaagcagg tgggctcaaa gatcgcctat
     2161 ggagaaaaca cggctaccct gaccttcggt cctatcgatc ttccaaccgg ccatggtgaa
     2221 ggcgatatgg gcgactccat gcctcgattc aacttccagt tgcctgagga caaatatttg
     2281 gtcggcttca agtcggctct ttccacagtc gacgggaagg agttgccgag aaattacctg
     2341 caccatattt tgatgatcaa caataccaag cccagcgtct cctgccccgg tgagccgctt
     2401 ttcttcggtg gggccggact cgaaatgtcc gagacccaat tcccagaggg atacggcgtg
     2461 aagctgtcgg caaccgacaa attaatgact gtcgtcgcgt tctatcacgg cgcccccccc
     2521 accaaagacg tcatcgcaac gttcacgatg tattttgcgc cgaaggtcaa accggtcaag
     2581 gagatggaga tttatcaggt cggcgtcaac attgtctgct tcacgaagtt cggcgatcgg
     2641 ccggcggatc agacggacga agggatcgaa atcgggccgg gtgtccaggt ccgtacggcc
     2701 ccattgaagt ttagtatgga tggttgcgtc aaatacgcgt atccgcacgg ccacgatgaa
     2761 ttgctcctga ttgcattgga gaataagacc aagagacaga ccctcctccg gactgtgccg
     2821 gatgttgaac cggacggaac gctccgcgaa ttccttcctc atcaagtcta taaagacgtc
     2881 cagggattcc ctatttccaa agaagacgac tatgagatgg tgatggtcta ccaccattct
     2941 ctacagagaa ctgaactcca acatggcatg ggcaactatc tgctgtatat gacgcccggc
     3001 gcctgctcag ggcagggcaa aactgccgcc gcctactgat cccctcgcag gcctgccgga
     3061 caaaaccatc cgacaggcct gcgaacgatc cgctgttttc ctaccgtcca aaattgttca
     3121 ttgaccgcct cgtagctacc gtcccctacg aaagaatcgg cccccagtct gggtttctcg
     3181 ccgccacttg tggtatcaag tcccagggtc ctgaagcatc agcggtgttg tgtgactgaa
     3241 tcggctgatt gacacgcggc gatatgcagc agcccgctga ataacctccc cgagagcagg
     3301 cttgcccaga aacacgggaa tcgtgccatg ccgcagagtg agcctgtcgc catccttctc
     3361 gtcaatgcga tcgccgaaga aatcaaactg gccacgctca acttccgaag attctttcca
     3421 aactgccgcg tcgaagccgt ctataccgtg gaggaagcgc ttcagtgggg acaacgggca
     3481 tcatggcagc tcatcttaat cgatgaggat ttgctcgccc agcgcgctgc gcccgtcttc
     3541 tcagaactga ggaggcttgt cccctatgcc acgctcgtgc tgcagacggc tcatagcgac
     3601 tccgcctcct ccttgaatgc gcttcaggca ggtgcggatt ttctccttta caagcagtcg
     3661 cccgctttcc tcgccgaact gatgttgtat acaaaaagcg cgctcgaaac acgtgccatc
     3721 cgtatgacgc tcaaacagac agaggaccgc cacgggtgtc tcatcgatat actcagtgat
     3781 ggattatatg aactcgatgg agaaggacga ttcgtgtacc tcagcccgct ggccactgaa
     3841 atgctgggct acacggagaa cgaattaatc ggcactctgt attccaccgt cgtacctccg
     3901 gatcaacagg atcgcgctcg tcatcgtttc aacgaccgcc gaacggggac gcgagccgcc
     3961 cggcggatag agattgatct cattcgcaag acatcctccg acaaaccggc ccatacacga
     4021 gtcagagccg agatcagtgc gcgagggctg tacgacttta atcgccggca cctcggaacg
     4081 ctgggtctgt tgcgagatat atcgcaccac cgccggcaaa cgaagaccat tgatcagctt
     4141 gagcatcaac ttcgtgaatc cgatcgactt gtcgccatcg cacggcgctt ttcaaccttg
     4201 tcgaaaaatc tgcacgcccc tcacaatgcg attcaaacgc agtctcaact cctgctcaaa
     4261 accatccgcg atgctcgcct gatcgaacaa gtcgactcgc tggcaacata cgccgctgaa
     4321 gccgcccatc tggggcaaga gctcgtccag accacgacgg aaatggtcac ccgcccagac
     4381 accatcaatg acattatcga gaccattctg gccgccgcta cacctccccc cacccttgcg
     4441 aacatcgagt ggattgaacg tgcctatgag cagaatcttc catctttcac cggaagcctc
     4501 gattccatga tggatctcat gcgcattctc ctcacacatg cccgacgcta tgtggtcgct
     4561 gtaggaactc cccatcgatt acgaatcagt accagggcaa tcaacgaagc cggagacccc
     4621 gtagcccaag agacatcttt gcttccatca acggcggccg ccgaattcga aattcacatc
     4681 caagaaacag acattatcat ctcggctgag gagccgcccc g
//
LOCUS       DATNDD010000479         4836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_1815124, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000479 DATNDD010000000
VERSION     DATNDD010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815124"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            523..2766
                     /locus_tag="VN647_11555"
     CDS             523..2766
                     /locus_tag="VN647_11555"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWG97711.1"
                     /translation="MRLLTLSLRFVLPLAIVLGVIAYGVIPFVDSMELKWFVRDLDMR
                     SKLMVNTMEVPLTDLLVSNSKEKILAYFTRIRQDERLYALGFCDLDHHLLYETQAYPQ
                     DVTCKDTLDLASNSSTVRSFSSGPLHITSASIESNGRRLGRLLLIHDMSFIQQRSSDT
                     KWYVFYLFAGLTAVISLITVLVAHFSWKEWVAGVRALLKGERLLTPLTQEQHAPELQP
                     LAKDLRSLVQALESDRRMRDESQISWTPASLKTILHEQLAGDQVLIVSNRQPYSHYWQ
                     DRNIVVQMPASGLVSALEPVMRACSGIWVAHGSGSADREVVDGRDHVGVPPSHPMYEI
                     RRVWLTEEEEAGYYYGFANEGLWPLCHIAHVRPTFRSSDWNHYVAVNERFAQAVYEEA
                     TTDNPVVLVQDYHFALLPKLIRDKLPTATIIMFWHIPWPNAESFGICPWREEILEGLL
                     GSSILGFHTRVHCNNFIDSIDRILEARIDRNSSTVSYGGKMTAINPYPISIEWPLQWL
                     SDQRPVPECRIKLREAYGMPPDRLVGLGVERLDYTKGILERFMAVERLLELQPEWIGK
                     FTFVQIAAPSRSTIEQYHHFTGQVSALAEQINKRFGRDGYEPICLRVQHHEASQVCEC
                     YRGADLCVVSSLHDGMNLVAKEFVGARDDEQGVLILSQFTGAARELTEAVVINPYDID
                     QFAAALHLGLTMPKVEQRARMQSMRGLIQEFNVYRWAGRMLMDAARMRQKERVMKHVR
                     RRPSRLS"
     gene            2777..3604
                     /gene="otsB"
                     /locus_tag="VN647_11560"
     CDS             2777..3604
                     /gene="otsB"
                     /locus_tag="VN647_11560"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWG97712.1"
                     /translation="MDYLLTETGRAELGALLKVRSLYAFDFDGTLAKIVREHHAARLA
                     SPTRLRLEELAKRAPTAIISGRSVEDLRSRVGTAVPHLIGNHGSEGPHTRPEDLRQVR
                     ETSLGWLQLINGRFQDELARRGVVVESKSFSLSFHYRTVDQRDEARALISRIVAELSP
                     PPRIVLGKSVVNVMPPTASHKGTALLEHMRRLDCSTALYVGDDETDEDVFALRDARIL
                     TVRIGKKNGSSARYFLKRQAEIAEVLRLLVEGGDQNIHTWSGCREQGIPQPTQHSNE"
     gene            complement(3958..>4836)
                     /locus_tag="VN647_11565"
     CDS             complement(3958..>4836)
                     /locus_tag="VN647_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HWG97713.1"
                     /translation="PGRFDRQVTVNHPDLRGRSEILKVHTKNVPVASNVDLEKIARGT
                     PGFSGADLANLVNEAALWAARQNKSEVGTIDFEMAKDKIMMGAERKSMVLTDQEKQVT
                     AYHEAGHALMAKLLPGTDPVHKVSIIPRGRALGVTMQLPTDDRHNHSQGFLYNTLAIL
                     LGGRVAEELVFQQVTAGAGNDLERATALARKMVCEWGMSDKLGPLTFGQKEESMFLGH
                     SFGGRREVSDTMALEIDLEIKRLVTENYERAKRILTEQMGSLQALAEALLEKETLDGL
                     EIDQIISNASSIAVPV"
BASE COUNT         1042 a         1349 c         1332 g         1113 t
ORIGIN      
        1 cccaagacgt ggccgaagga agagaacgat gaaaatcagg aacacttgcg catacatcag
       61 cggtctaggg tcacaattga atgcattcat tctaggccgc gaccgcgacc ctgcattatt
      121 caaagcgcag catcgacggc aaccatggtg gggtccccct cgcctccacc aaaccgcact
      181 tcatgcacac cgccggtctc tttatcgttt cttcgtaatt gaggccagcg gataaaaacc
      241 ttcagtcatc ttaccagttt gacctggaac tatctgatgg ctccaggcga cgagagagaa
      301 cacctccggt gatttcatga acaacggcta ctcgtttgcc ggactttcaa ctgcgtcatg
      361 agctatcgaa tccgggagct gttcggtctc ggaaggcaca atcagtcgat agactcttct
      421 gagttgatca gcgcggaaat tcgacaattc ccgatgagtc cgtgtgtgta taccgggcgg
      481 ccatatttga tttctgcatg taaagtaaac taacttgttt ctatgagact cctcaccctt
      541 tcgcttcgat ttgttctccc attggcgatt gtcttgggag taatcgccta tggagtgatc
      601 ccgttcgtcg actcaatgga gttgaagtgg tttgtccggg acctcgacat gcgatccaaa
      661 ctcatggtca atacgatgga ggtcccactc acagatcttc tggtgtccaa ttcaaaggag
      721 aagatcctgg cctattttac gcgcattcgt caggacgaac gattgtatgc cctgggattt
      781 tgtgatctcg accaccatct tctctatgaa actcaggcat acccgcagga cgtcacctgt
      841 aaagatacac tcgaccttgc atccaattcg tccaccgttc ggtcattcag cagcgggccg
      901 ctccatatca cgtcagcctc gatcgaatca aacgggcgcc ggctgggccg tctcctgctc
      961 atccacgaca tgagttttat ccaacaacgg agcagcgata caaaatggta cgtcttctat
     1021 ctattcgcag ggcttaccgc cgtcatctcg ctcatcaccg tgctggtcgc acatttcagt
     1081 tggaaagaat gggtggccgg ggttcgggct ttgttgaagg gagaaagact tttgactccg
     1141 ctgacgcagg agcagcacgc gccggaactc cagccgttgg cgaaagactt gcgttcgttg
     1201 gtgcaggctc ttgaatctga tcgccgtatg cgcgatgaga gccagatttc gtggaccccg
     1261 gccagcctca aaacgatcct tcacgagcag ctcgccggcg atcaggtgtt gattgtgtcc
     1321 aatcgacagc cctatagcca ctattggcag gatcggaaca tcgtcgtgca gatgccggcg
     1381 agcgggcttg tgtcggcgct tgagccggtg atgcgtgcct gctccggtat atgggtcgcg
     1441 catgggagcg gatcggcgga ccgagaagtg gtggatggac gggaccatgt cggtgtgccg
     1501 ccgtcgcatc cgatgtatga gattcgccgc gtgtggctca ccgaggaaga agaggcaggc
     1561 tactattacg ggtttgccaa cgagggattg tggcctctct gtcatatcgc ccatgtccgg
     1621 cccacgtttc gttcatcgga ttggaatcac tatgtggccg tcaacgagcg atttgctcag
     1681 gcagtgtatg aagaggccac gaccgacaat ccggtcgtct tggttcagga ttatcatttt
     1741 gccctgcttc cgaagctgat tcgagataaa ttgccgactg cgacgatcat catgttctgg
     1801 cacattccat ggccgaacgc ggaaagcttc ggcatctgtc cctggcgcga agaaattctg
     1861 gaagggctgc tgggaagcag tattctgggg ttccacaccc gcgtgcactg taacaatttc
     1921 atcgatagta tcgatcgtat tcttgaggcg cggatcgatc gaaacagttc aacggtctcc
     1981 tatgggggta aaatgacggc gatcaacccc tatccgattt ccatcgagtg gccgttgcaa
     2041 tggctttctg accaacggcc ggttccggaa tgccgaatta aactccggga ggcttacggg
     2101 atgcctcccg atcgtctcgt cggtcttggg gtggaacgtc ttgattatac gaaggggatt
     2161 ctggaacgat tcatggccgt ggaacggttg ctggagctcc agccggaatg gatcgggaaa
     2221 tttacgtttg ttcagatcgc agcccccagc cgatcaacga tcgagcagta ccaccatttt
     2281 accggccagg tctctgcact ggcggagcag atcaacaagc ggtttgggcg ggacggctat
     2341 gaaccgatct gcctgcgggt tcaacatcat gaggcctctc aagtctgcga atgttaccgt
     2401 ggagcggacc tgtgcgtggt gagcagtctc catgacggca tgaatttggt ggccaaagag
     2461 ttcgtcggcg ctcgggatga tgagcaggga gtattgattc tgagtcaatt cacgggagcc
     2521 gctcgtgagc tgacggaggc ggtggtgatc aatccctacg acatcgatca atttgcggct
     2581 gcgctccacc ttggcttgac gatgccgaag gtggagcagc gggccaggat gcagagcatg
     2641 cgaggattga tccaggagtt caatgtgtat cgctgggccg gtcgtatgct catggatgcg
     2701 gcccgcatga gacaaaagga gcgagtcatg aaacatgtgc ggcgccggcc gagccggctg
     2761 agctgatggg cggacgatgg actatttatt gacagaaaca ggaagggctg aactcggtgc
     2821 gcttttgaaa gtacggtctc tgtacgcgtt tgattttgac gggactttgg cgaaaatcgt
     2881 ccgagaacat catgcggcaa ggctggcgag ccccacccgc ctccggctcg aggaactcgc
     2941 gaaacgcgcc cccaccgcca tcatctcggg acgctcggtg gaggacttac gttcacgtgt
     3001 ggggaccgcc gtgccccacc tgatcgggaa ccatggttca gaagggcctc atacgcgccc
     3061 agaggatctg cggcaagtcc gggagaccag tttgggatgg ctgcaattga tcaacggacg
     3121 atttcaggac gagctggctc gacgcggcgt agtcgtcgaa agtaagtcct tttcactctc
     3181 attccattat cgaaccgtcg atcagcgaga cgaggcgcgg gcgctgatct cgcgaatcgt
     3241 tgccgaattg tctcctcctc cccgcattgt ccttgggaaa tccgtcgtca atgtgatgcc
     3301 gccgacggcg tcgcacaagg gtacggcgtt gctggagcac atgcgccgac ttgactgcag
     3361 cacggctctc tacgtgggag acgacgaaac cgatgaagat gtctttgcgc tacgagatgc
     3421 ccgcattctc accgtgagga tcggcaagaa gaacggatcc tccgcccggt atttcctaaa
     3481 aagacaggcg gaaatcgccg aggtgctcag acttctcgtc gagggcggtg atcaaaacat
     3541 tcacacctgg agcggctgcc gcgaacaagg aatccctcag cctactcagc attccaacga
     3601 atagatccgt caggatgttt aaaagtcctc cggcttcgtc ctcgatgcac acggaaagct
     3661 agccgttcta ttccaattcg atgacgaaga agctcttgtt tctcaacggc tacacgcccg
     3721 atctcatcga ggcggccgca gcaatccttg agaagattta ccgcgccgat cacgcctacc
     3781 agaaatgcga cgccatgctc accgatctga ttccggcgga cagtttccac atgatttgtt
     3841 cgatgaccgc gaccggcatc gtctctctcc agaatgctcc gcagccaagt tggctgcgga
     3901 gcaacgcagt ttgcgcaata tggccggtca gccatcctca cgataggccc tgccgtttta
     3961 gacaggaacc gcgatcgagg atgcgttcga aataatctgg tcaatttcca acccatccag
     4021 cgtctctttt tccaacagag cttctgccag cgcctgcaag ctccccattt gttcagtgag
     4081 gattcgcttg gcccgctcat agttttctgt gacaaggcgc ttgatctcca gatcgatctc
     4141 caacgccatt gtatcactca cctcacgcct gcccccaaag gagtgtccca gaaacatcga
     4201 ctcctctttc tgcccgaacg tcagcggccc cagcttgtca ctcatgcccc attcgcagac
     4261 catttttctt gcaagagccg tcgcacgctc caaatcgttg cctgccccgg ccgtgacctg
     4321 ttgaaacacc agctcttccg ccacccttcc acctaggaga atcgccaacg tgttatacaa
     4381 aaatccttgg gaatgattgt ggcggtcgtc ggtcggcaat tgcatggtca cgcctaaagc
     4441 gcggcctcgt ggaataatcg atactttgtg gaccggatcg gtaccaggca gtaatttggc
     4501 catcaaggca tggcccgcct cgtgataggc ggtgacctgc ttttcctggt ccgtgaggac
     4561 catgctttta cgctcggcac ccatcatgat cttgtctttg gccatttcaa agtcaatggt
     4621 tccgacttcg cttttgttct gacgggctgc ccagagggca gcttcgttga ccaagttggc
     4681 caaatcggcc ccggaaaacc cgggggttcc acgtgcaatc ttctccaggt ccacgttaga
     4741 tgcaaccggc acgttcttcg tgtggacctt caggatctcc gaacggcctc gcaggtccgg
     4801 atgattcacc gtcacctgcc gatcgaagcg gcctgg
//
LOCUS       DATNDD010000480         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2826744, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000480 DATNDD010000000
VERSION     DATNDD010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2826744"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VN647_11570"
     CDS             complement(<1..744)
                     /locus_tag="VN647_11570"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWG97714.1"
                     /translation="MTKSPRALIILSMGLFVLGLIGVAPVSSETQEKEATPEKPPPRA
                     QHPPAASDGQPKPSTETKPQSQPNPATKADSSTDHKEAMPEKPAPRAPHPPAASEGQP
                     VPSKPATEAKPQSQPPPAIKTDVPPAEPESKEKDSVVKKPLGSLILSVKLALMGDSRL
                     FRYEIEVGDDQQTVTLTGRVSTEEEKTAATEIAQAVPGVKTVVNKLAVEKDLAKTLLK
                     KQDEILTTLIKERFSKSATLKAANFEVKTE"
     gene            complement(910..2229)
                     /locus_tag="VN647_11575"
     CDS             complement(910..2229)
                     /locus_tag="VN647_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWG97715.1"
                     /translation="MSDALADSKETGSAPSRARGWELVKTRDFGFLFAGQTISQIGDS
                     LNKVALLWFVYEMTGSALKMTVVGLLQTLPPLLLGPLIGVYLDRVRKKPVMIGVDLLR
                     TLMVLLIPLLYAMGGLTLDRLYFLVFATAIFSTVFGPALTSAVPLIVPKERLIAANAL
                     LQTTTNVGLLVGPAVSGLGIALIGAQNVLYADAATFFISALCLFPIRMHETLDHWRTA
                     KKGLTEGITTDLLAGFRFVFVEQKTVLLLMLTATLYSVGISAFIFLLPVFAKEVLGVG
                     PIQLGWLWSALGVGMLLASLGLTSITQGDVSWRLRFMSGALAVGGLAVAALGFLEAPV
                     VAGALIAVIGGSTAMFTPMVWTMLQELTPAHLLGRVFTSFATGGMASSMAGMAGFGWA
                     ADTIGPAASLGGISLILLMTAATAWLFSFYKSHTGSLAISTSSPLAS"
     gene            complement(2222..>2869)
                     /locus_tag="VN647_11580"
     CDS             complement(2222..>2869)
                     /locus_tag="VN647_11580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWG97716.1"
                     /translation="NWAATVHHGLPRNLYTFHPQAQGYLAFLGRIAPEKRPDQAIEIA
                     KRAGLPLRIAAKVDPADQNYYEAEVEPLLNHPLIEFIGEISDAEKDEFIGNATALVCP
                     YDWPEPFGLVLIEALACGTPVLAYRRGSIPEILDHGVTGFICETVAEMVDAVGQVPLI
                     DRRRCRAAFDERFTADRMARDYVALYERLLEDEAVQATPAFASEQHKILGRGRHV"
BASE COUNT          628 a          813 c          831 g          597 t
ORIGIN      
        1 ctcagtcttc acctcgaagt tggctgcttt taaggtcgcg cttttggaga aacgttcttt
       61 gatcaaggtg gtgagaattt catcttgttt cttcaacagc gttttcgcga ggtctttctc
      121 gactgcaagc ttgttgacca cggtcttgac gcctgggacg gcttgcgcga tttccgtcgc
      181 ggccgttttt tcttcttcgg tcgagacacg tcctgtcagg gtcacggttt gctggtcgtc
      241 tcccacttca atctcgtagc ggaaaagccg tgaatccccc atcaacgcaa gcttgaccga
      301 cagaatcagt gaacccagcg gcttcttgac aacggaatcc ttctccttgc tttcaggttc
      361 agccggtggc acgtcggttt ttatcgccgg aggtggttgg gactggggct tcgcttccgt
      421 cgccggtttg gaaggcacgg gctgaccctc tgaagcagcc ggggggtgtg gcgcgcgtgg
      481 tgccggcttc tccggcatag cttctttgtg atcggttgac gaatccgctt ttgttgcagg
      541 attgggttga gactgcggct ttgtttccgt cgagggtttg ggttgaccgt cggaagcggc
      601 cggaggatgt tgcgcgcgtg gtggcggctt ctccggcgta gcttcctttt cttgtgtctc
      661 cgaggacact ggggccacac ctattagccc cagcacgaac aggcccatac tcagtatgat
      721 cagcgctcgt ggcgacttgg tcatcgtggg agcccctcct cgtgacgata ttcctgagaa
      781 ttcatccagc cattctaact cgcgacggct ttcgtgtcat ctattcccgg agcgagacgg
      841 ccccctgtgt ctcagagggc cgttccgggg tgatgcgtct gttgatggaa acttcgagac
      901 gatggcgggt taagaggcca aggggctgga cgtagaaatg gccaagctcc ctgtgtgcga
      961 cttgtaaaag ctgaacagcc atgcggtcgc agccgtcatg agaagaatga ggctgatgcc
     1021 tcccaggctg gcggccggac cgatggtatc ggcagcccat ccaaaacccg ccataccggc
     1081 catggatgac gccattcctc ccgtggcgaa gctcgtaaag actcggccca ggagatgcgc
     1141 cggcgtcaat tcttggagca tcgtccacac catcggagta aacatagcgg tgcttcctcc
     1201 gatcactgcg atcagggcac cggccacaac aggagcctct aggaaaccga gcgccgcgac
     1261 cgccagaccg ccgacggcta aggcaccgga cataaaccgc aaccgccaag atacgtctcc
     1321 ctgcgtgatt gatgtcagcc caagggaggc aagcaacatt ccaacgccga gggcggacca
     1381 tagccacccg agctgaatcg ggcccacccc cagcacctcc ttagcaaaga ccggcagcaa
     1441 aaagatgaag gcactgatgc cgacgctgta gagagtcgcc gtcaacatga gaagcaagac
     1501 cgttttctgc tcgacgaaga cgaaacgaaa acccgccagc aagtccgttg taatgccttc
     1561 cgtcaatcct ttcttcgccg tacgccagtg gtcgagagtt tcatgcatac gaatggggaa
     1621 taaacacagt gcggaaataa aaaaggtcgc cgcgtcggca tagagcacgt tttgtgctcc
     1681 gatgagcgcg ataccgagac cgctgaccgc aggccccaca agaagtccga cgttggtcgt
     1741 cgtttgcagt aacgcattgg cggcgatgag ccgctctttg ggcacaatga gaggaaccgc
     1801 agaggtcagg gccggcccga agaccgtgga gaagattgcg gtggcgaaca cgaggaagta
     1861 gagccggtcg agagtcaggc cccccatcgc gtagagcagt gggatgagga gaaccatcag
     1921 ggttcggaga agatccactc cgatcatgac cggtttcttt cgaacgcgat ccagatatac
     1981 accaatcagc ggccccaaca gcaagggcgg cagggtttga agtagtccga ccaccgtcat
     2041 cttgagcgcc gacccggtca tctcatacac aaaccagagc agcgccacct tgttgaggct
     2101 gtcgccgatc tgagagatcg tctggccggc gaataaaaag ccgaagtctc gggttttaac
     2161 taactcccag cctcgagcac ggctcggagc ggaaccggtc tcttttgaat cagcaagtgc
     2221 gtcagacatg tcggcctcgc ccaagaatct tatgctgctc tgaggcgaag gcgggggtgg
     2281 cttgaacagc ctcatcttcg agaagccgtt catacagcgc tacatagtcg cgcgccattc
     2341 gatccgcagt aaaccgctcg tcgaaggcag cgcggcagcg ccgccgatcg atgaggggaa
     2401 cctgtccgac tgcatccacc atttcggcta cagtctcgca aataaaaccg gtgacgccat
     2461 gatcgagaat ttccggaatc gatccacgtc ggtaggctaa caccggagtt ccgcaagcca
     2521 gggcttcgat caataccaat ccgaaaggct ccggccaatc gtaaggacac accaatgccg
     2581 tcgcattgcc gataaactca tctttctcag catcggagat ctctccgatg aactcaatca
     2641 agggatggtt gagcaacggc tcaacttcgg cttcatagta gttctggtcg gcaggatcga
     2701 cttttgccgc aatccgcaga ggaaggccgg cgcgtttagc gatttcaatc gcttggtccg
     2761 gacgtttttc aggggcgatg cgccccagaa aagccagata cccttgggcc tgaggatgaa
     2821 acgtgtagag gttgcgagga agaccatgat ggacagtcgc cgcccagtt
//
LOCUS       DATNDD010000481         3588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_754038, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000481 DATNDD010000000
VERSION     DATNDD010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754038"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VN647_11585"
     CDS             <1..349
                     /locus_tag="VN647_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWG97717.1"
                     /translation="RTNYRLFAGGPVEQTQLVLLFRLTQLLPDTRQIVAGIYVGTPRV
                     LERIMTQPKPTETFRAFAGFAGWAPGQLEHEMLEGAWAVLPSDMFSIFDKDPATLWPD
                     SISHLQAPKTISH"
     gene            754..1692
                     /locus_tag="VN647_11590"
     CDS             754..1692
                     /locus_tag="VN647_11590"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HWG97718.1"
                     /translation="MEQAVHTSGTAALRAKLRNTWLRDLVVQYRDIKRRVMRRSEGEA
                     VLLHRYVRIHGKPLNLTNPQTFTEKLFWRMITWNRGNMPPRFRQLADKYAVRAHVAKT
                     VGEEYLIKLLWQGSDPRAIPFDRLPAEYVIKPNHSSGEVIIVRGEANREGIIRQVSSW
                     LAKNYYWQAREYQYYGIKPKIVIEEYLKSQDDSEPLDYRFWCFRGVPAVIQVDNHAHD
                     INSFFDAQWNQLDLHTRKKATRPPIAQPTCFEHMFELASRLSQGIGFIRVDLYNTNGR
                     IYCGELTLTPGAGIFQFGPDHWDLKLGEKWDLALDN"
     gene            2679..>3588
                     /locus_tag="VN647_11595"
     CDS             2679..>3588
                     /locus_tag="VN647_11595"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HWG97719.1"
                     /translation="MEYSVYAPVTAAVRAKLQDSWLHDLVVQYRDIRRRVMRRSEGEE
                     FLLRRYSRIHGKPLNLTNPQTFTEKLFWRMITWNRGDIPLRFRQLADKYAVRAHVANT
                     VGEEYLTKLLWHGDNPRAIPFDRLPVEYVIKPSHAAGQVLIVKGRADRNEIIRTVSGW
                     LANDYYWHGRESQYYRIPPRILIEEYLTNEDGSPPFDYKVYCFNGTPEQIMVRNHTHD
                     ICPFFDTTWNFLDFSDEIGAVRPWVPKPANLAEMLTLAAKLSVGFGYVRLDFYNVKGR
                     VYFGEFTFTPAAGIITYDPECWDLKLG"
BASE COUNT          870 a          892 c          954 g          872 t
ORIGIN      
        1 gcgaaccaat taccgattgt ttgccggtgg gccggtcgag caaacgcaac tagtcctgtt
       61 gttccggctt acacagctct tgcccgacac gcgccagatc gtggccggaa tctatgtggg
      121 cacgcctcgt gtcctggaac gtatcatgac tcagcccaaa ccgaccgaaa cgtttcgagc
      181 gttcgccgga tttgctggat gggcgcctgg acagctcgaa catgaaatgc tcgaaggagc
      241 atgggccgtc ttgccgtcag atatgttcag tatcttcgat aaagaccctg ccacgctatg
      301 gccggacagc attagccatc ttcaggctcc caagaccatt tctcattgag tgatcgagag
      361 aaaccttctt cgagctgcct actacctacc taaaaatggt actatgcctc tcttcgctat
      421 tagcataaag tctcactctc gaccgtactc tcattcatac atccaccatc ttcctatata
      481 tgctaaacca tacatgtact tctcgggata gtcctagttc acactgcgtt tctttcatcg
      541 tcatcatcaa gacgcccgag gagttcattt ttgcttgctt tccaagattc ctttctctaa
      601 caaggatggg catgcaatca ctcaaggtat aggccgcaca gattctagtt gctttttatc
      661 tgagcaggga ctctttgtag aaccacaaga ctttctaaga ctctctgaca gagagtgcga
      721 gttttgcgag ggatatagag gagggatata gagatggaac aagccgttca tacatcggga
      781 accgcagcgc ttcgcgccaa gttacgaaat acgtggttgc gtgacctcgt cgtacaatat
      841 cgggatatca agcgccgggt gatgcgacga tccgaaggcg aagccgtgct gttgcacaga
      901 tatgttcgga tccacggcaa accgctcaat ttgacgaatc cccagacctt tacggagaag
      961 ctgttctggc gcatgattac ctggaatcgg ggaaacatgc ctcctcggtt tcgacagctg
     1021 gccgataagt atgcagtgcg agcacatgtg gcaaagacgg tgggcgagga gtatctgatc
     1081 aagctcttat ggcaggggag cgatccgcgc gcgattccgt ttgaccggct cccggcggag
     1141 tacgtcatca agccgaacca ctccagcggg gaagtaatca ttgtccgagg tgaggcaaat
     1201 cgcgagggaa ttatccgtca ggtgtcgagt tggttggcca aaaactatta ttggcaggcc
     1261 cgcgagtatc aatattatgg gattaaaccc aagattgtga tcgaggagta tttgaaaagc
     1321 caggatgaca gtgagcctct cgactacaga ttttggtgtt tcagaggagt tccagcagtt
     1381 atccaggttg ataaccatgc ccacgacatc aactcctttt ttgacgccca atggaatcaa
     1441 ttggatctcc acactagaaa aaaagcgact cggcctccca tcgcacagcc cacgtgcttt
     1501 gagcacatgt ttgaactggc aagccgacta tcacagggaa tcggtttcat tcgcgtcgat
     1561 ttgtacaaca ccaatgggcg gatctattgc ggtgaattaa ccttaacgcc gggcgcgggg
     1621 attttccagt ttggtcccga ccattgggat ttgaaactcg gagagaagtg ggatctggca
     1681 ctggacaact gatggtcgaa cctgctgggc cggtaacgag ttgccgcata agttgcgacg
     1741 atacttcgcc gttgcagatg ttgtttctac atttcggatg atcaagattc ctaccaaaat
     1801 tctgtaaagt acctccttaa ctgctggtga tcaaacgcca gcgatgtgcg atagatcctt
     1861 ttttgagggg tgtggagccc ccggctttac agccgtgtcc ctagtgattc ttcggcgaca
     1921 cctcgccgaa gggagtcctc aagtcttcgg aagatacccg ctgctcctcc acagcttcac
     1981 aaccgtgact tcccgcgtag gcgggtgaat tggaagtttc accaaagcta cagtgcgtca
     2041 gtccccaata ggccacgaag ctgttctggc gcatgattac ttggaatcga gacgcgatat
     2101 acccctccgg tttcgacagc tggttgataa gtattaagta tgcggtacgg gcacatgtgg
     2161 cgagcatggt gggcgaagag tatctgacca agctgatgcg cgccgcatcg ccatgaggta
     2221 tgacaaatcc gccaaaaaat acctcaacgc tatctgcctg atgaccctcc tttactggtg
     2281 gctcaattga gtctggaccc tagggaagtg agcacgatag atcggctagg gaaagtgctg
     2341 tgagattagc ttggacatat cagggttcgt aggcctgttc caatgtgcag gtcgaacgtc
     2401 cgttgtctgg tttctttaaa ggacacatcg ggacatgatc ccggggaata gaacggctga
     2461 tcagaacctg gcgaccgggc agaggtggtt ttttcaagta gcactttggt agggataatt
     2521 ccgaagggaa aagatgggtt catgctatat aatgagcgcc ataagtcatg cgttcagcac
     2581 cggccatcct aatgtctgat gacttcacct ccaagcaggg acggttcttg aatcggagaa
     2641 ctcactgaca agaagtaaga attttgcgag aggtaggaat ggaatattct gtctatgcac
     2701 cagtaactgc agcagttcgc gccaagttac aagattcctg gctccatgat ctcgtcgtac
     2761 agtatcggga catcagacgc cgggtcatgc ggcgatccga aggggaggag ttcctgttgc
     2821 gcagatattc tcgaatccat ggcaaaccgc tcaatctgac gaatccccag acgtttaccg
     2881 agaagctgtt ctggcgcatg attacctgga atcgaggcga catacccctg aggtttcgac
     2941 agctggccga taagtatgcg gtgcgagcgc atgtggcgaa cacggtcggc gaggagtatc
     3001 tgaccaagct cctatggcat ggagacaacc cgcgcgcaat cccctttgat cggctgccgg
     3061 tggagtatgt gatcaagccg agccacgccg ccggacaagt gctcatcgtc aaggggcggg
     3121 cagatcgcaa cgagatcatc cggaccgtgt cgggctggtt ggccaacgat tattattggc
     3181 acggacgcga atctcaatac tatagaattc cacctcgaat tctgattgag gaatacctga
     3241 ccaatgagga tgggagtccg ccgtttgact ataaagtcta ctgttttaac ggaacgccgg
     3301 agcagattat ggttcgtaat catacacacg atatctgccc attttttgat accacatgga
     3361 atttcctcga cttttccgat gagataggtg cggtacgtcc gtgggtacca aagcctgcga
     3421 atcttgccga gatgttgacg cttgccgcga agctgtcggt cggcttcggc tacgtccgct
     3481 tagatttcta caatgtcaaa gggcgagtct attttggtga gtttaccttt acacccgcgg
     3541 cagggatcat cacatatgat cctgagtgtt gggatctgaa gctcgggg
//
LOCUS       DATNDD010000482         4728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3431620, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000482 DATNDD010000000
VERSION     DATNDD010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3431620"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3460
                     /gene="mfd"
                     /locus_tag="VN647_11600"
     CDS             <1..3460
                     /gene="mfd"
                     /locus_tag="VN647_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWG97720.1"
                     /translation="NTPTPSWLAPLHSALGREQTRLCLLGAHGSTAACALTLLNETRQ
                     STPDRARPWVVVTTNDDSAERMFNDLSFFHELMGRPVEDLAWFPEWETLPYEATAPHV
                     GLIAHRMTTLHRLLVNPPTILVTSVAAAMHRVIPRSIFEQAVFRFETAAAFEREPLIT
                     NLLRLGYRRVSVVEIPGEFSIRGGIVDIFSTAYADPIRVEFLGDQVESIRLFDASTQT
                     SIEKLKDGWVLPAREFVRPPDDPDAIMSIPADAEWRSPDLYTSMDTLFDYLTAAPFLA
                     LDQPDALRKACEAAWEKIDDGYLRHVDRDATHPYPSPERLFLTWPGIQQRIETWPMLA
                     LEPLAAPDSTWNQTFSFSAQAPGSIGLGIRGTAFSQTLSLLEGLRNEHRVVLVARSRG
                     QVDRLLALLREHDLPADPWKPTVWSGHRTGKLPFYVLHGDLSAGFLSGDLRLALLTEE
                     ELFAKGARHKPQPKSRTATFLSSLEDLNVGDYVVHVQHGIAKYRGLKRLSIQDYESDF
                     LILEFSGGDTLYVPLDRLNQVQRYSGAESHVPRLDRLGGTSWAKTTARVKKDIEEMAH
                     ELIDLYANRELVKRNAYGTTTTLYHEFEAAFEYEETADQLRAIEDIGRDMESTKPMDR
                     LVCGDVGYGKTEVAMRAAFKAVEHDRQVAVLVPTTLLGHQHYENFAERFAPFPMRVAL
                     LSRFQSPRETKTILKDVAAGTVDIVIGTHRLLQKDVVFRQLGLVIIDEEQWFGVKHKE
                     RLKQLRTQVDVLTLTATPIPRTLQMAMSSVRDLSIIDTPPAGRLAIKTEVIRFSDKAV
                     RDAILRELGRGGQVYFVHNRVETMERIGAWLHQLVPEARMVMAHGQMDARPLEAVMLK
                     FVKREADVLIASAIIQSGLDVPNANTIIVNRADLFGLAQLYQLRGRVGRGGEQAYTYF
                     LIPDEGTLTGDAQKRLIAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHIAAIGL
                     DLYMQMVEQAVQRLKGHVMEEEPDPTLQFPVSAFIPEQYVADPHHRLTLYKRLTACGQ
                     VGELALLHGEIQDRYGPPPEPVERLLEVMQLRIHAKRLHLASIEVHGQTAKVVFHPKT
                     VIPETSIHRLMDQLKKRLRFLSPASFEVQMRDGDWPALFSELNAILQSLDLCDTKTIK
                     KEATAS"
     gene            3549..4382
                     /locus_tag="VN647_11605"
     CDS             3549..4382
                     /locus_tag="VN647_11605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWG97721.1"
                     /translation="MLLGMGPVVSGCAERQEEPVVALVNGRAITQAEFDLRWGELSKA
                     TRSRYEKEGGKRRFLDELITRELLMQEARRRGLDQDDTIRDKTQRYKEQLILDELLKD
                     KLQSKVEVTQAELDAYYEKHANQLLDPLKADVSLMLLPNVYAARDLEAQMHRGGSFVK
                     FAQRYSIDEKTKHKGGELGPYRKGLVPPEVDAVIHTLKIGMVSAPIKTDNGYYLVMLT
                     PLDEAIIQADLATQERLRQELLSDKRRKRFDEDIADIRANAVVRFAGASRHITEDTGK
                     R"
     gene            4455..>4728
                     /locus_tag="VN647_11610"
     CDS             4455..>4728
                     /locus_tag="VN647_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97722.1"
                     /translation="MKRVSLRSDRPVRFKLPLVLLVITTWLPAISEARLQDRIVAIVN
                     SELIMLSDVKREFETEHERLSREHRGNDLPQRLKTAEYMALTKLIER"
BASE COUNT         1033 a         1403 c         1327 g          965 t
ORIGIN      
        1 caacaccccc accccatcct ggctcgctcc gcttcattcg gcacttggcc gggaacaaac
       61 tcgtctctgc ctgttgggag cccatggctc gactgcagcc tgcgccctga ctctgctgaa
      121 cgagacccgc caaagcactc ccgaccgcgc ccgtccatgg gttgttgtga cgacgaacga
      181 cgactccgct gaacgaatgt tcaatgactt gtccttcttt catgaactca tgggtcggcc
      241 ggtcgaagac ctcgcctggt tcccggaatg ggaaacgctc ccttatgaag cgacggcgcc
      301 tcatgtcgga ctgattgcgc accggatgac gacgctccat cgtctgttgg tcaacccacc
      361 caccatcctc gtcacctccg ttgccgctgc gatgcaccgt gtcattccgc gctcgatatt
      421 cgagcaggcg gtctttcgct tcgaaacagc ggcagccttc gaacgagaac cgctcatcac
      481 caacctcctt cgcctgggat accggcgcgt gtccgtcgtg gaaattccgg gggagttcag
      541 catccgtggc ggcatcgtgg atatcttctc gaccgcctac gctgatccga tccgtgtgga
      601 atttctcgga gaccaggtcg aatcgattcg tttgttcgat gcttcgaccc agacctccat
      661 cgaaaaattg aaagatggct gggtgttgcc cgcgcgggag ttcgttcgcc ctccagacga
      721 cccggatgca atcatgtcga tcccagcgga tgctgagtgg cgaagccctg acctgtacac
      781 atccatggac acactgttcg actatctcac cgccgctccc ttcctcgccc tcgatcaacc
      841 ggatgcgcta agaaaagcat gtgaggcggc ctgggaaaag atcgacgacg gctatctccg
      901 tcatgtagac cgtgatgcca cgcaccccta tccctcgccc gaacgcctct tcctgacctg
      961 gccgggcatc caacagcgga tcgagacgtg gcccatgctg gcccttgagc cgctggcggc
     1021 gccggactca acctggaatc agactttttc gttttccgcc caagcacctg ggagtatcgg
     1081 actcgggatc agagggaccg ccttcagcca aacactgagc ctgttagaag gactccgaaa
     1141 cgaacatcgg gttgtcttgg tagcgcgaag ccgggggcag gtcgaccggt tgctcgccct
     1201 cctcagagag cacgatttgc ctgccgaccc atggaaaccg acggtctggt ccggccacag
     1261 gaccggcaag ttgccgtttt atgttctgca tggcgatctc tcggcggggt tcctctccgg
     1321 agaccttcgg cttgccctcc tgacggaaga agagttattc gcgaagggtg cccgccataa
     1381 accgcagccg aaaagcagaa cggcgacctt cctctcttcc ttggaagacc tcaacgtcgg
     1441 tgactatgtt gtgcacgtgc agcacggtat cgccaaatat cgagggctca aacgactctc
     1501 gatccaagat tacgagagcg acttcctgat tctcgagttc tccggtggtg atacgcttta
     1561 cgttcctctc gatcggttga accaagtcca acggtacagc ggagccgaaa gccatgtccc
     1621 gcgtctcgat cgcttgggtg gcaccagttg ggccaaaact accgcgcggg tgaaaaagga
     1681 tatcgaggag atggcccatg aattgatcga tctctacgca aaccgggagc tggtgaaacg
     1741 gaatgcgtat ggcacgacta cgactctcta tcacgaattc gaagcggctt ttgagtacga
     1801 ggaaacagcc gatcagctca gggccatcga ggacatcggc cgcgacatgg agtcgacaaa
     1861 gcccatggac cgactcgtct gcggcgacgt cggatacgga aagacggaag tggccatgcg
     1921 cgccgccttc aaagccgtgg agcatgaccg ccaagtggcg gtgctggtcc cgaccactct
     1981 gctcggccat caacactatg agaactttgc cgaacgattc gcgccctttc cgatgcgcgt
     2041 cgcgctgctc tcacgatttc aatctcccag ggaaaccaaa acgattctca aagatgtcgc
     2101 ggccggaacc gtcgatatcg tgatcggcac gcaccggctg ctccagaaag acgtggtatt
     2161 ccggcaactc ggcctcgtga ttatcgatga agagcaatgg ttcggtgtca aacataagga
     2221 gaggctgaag caacttcgca cccaggtcga cgtcctgacg ttaaccgcca cccccattcc
     2281 gcgcaccctc caaatggcga tgtcgagcgt gcgggacctc tcgatcatcg atactccacc
     2341 cgccggacga ttggcgatca agacggaggt gatccggttc agcgacaagg cggtgcggga
     2401 cgccatcttg cgtgaactcg ggcgtggggg acaagtctat tttgtccaca atcgggtcga
     2461 aaccatggag cggatcgggg catggctgca ccaattggtt cccgaagcgc gtatggtcat
     2521 ggcgcatggc cagatggatg ccagaccgct cgaagccgtg atgctgaaat tcgtgaagcg
     2581 cgaggcggac gtcttaatcg cctcggccat catccaatcc ggactcgatg ttcccaatgc
     2641 caacaccatc atcgtcaatc gggcggatct cttcggcctg gcgcagctgt accagctgcg
     2701 cggacgagtc ggacgcggcg gagagcaggc ttacacctat ttcctcatcc ccgatgaagg
     2761 cacgctcacc ggcgacgctc agaaacgatt gatcgcgatt cagcaattca cggaactcgg
     2821 atcgggcttc cgtatcgctg cggcagacct tgagattcga ggagcaggga atctcttggg
     2881 caaacaacag tcgggccata tcgccgcgat cggcttggat ctctatatgc aaatggtgga
     2941 gcaggccgtc cagcggttga aggggcatgt gatggaggag gagcctgacc cgaccctgca
     3001 attccctgtt tcggcgttta ttccggaaca gtatgtggcg gatccgcatc accgtctgac
     3061 tctctataaa cggctgacgg cttgcggcca agtcggggaa ctggccctgt tgcatgggga
     3121 gatccaggac cgttacggtc cccctccgga gccggtcgaa cgactgcttg aggtgatgca
     3181 actcaggatt cacgccaaac gactgcactt ggcctcgatc gaggtgcacg gccagacagc
     3241 caaggtggtg ttccacccga agacggtcat tcctgagacc tccatccatc gattgatgga
     3301 ccagctcaag aagcggctac ggttcctgag cccggcctca ttcgaagtcc aaatgcgtga
     3361 cggcgattgg ccggcgcttt tttcggaact caacgcaatc ttgcaaagcc tcgatctctg
     3421 tgataccaag accatcaaaa aggaagcgac ggcctcttaa tcgagagcca cctgtgccac
     3481 gacttatgat gcccttccgg tttccgacct tccgcaggac atattgtcta cttggactgc
     3541 ttgtcggcat gttgctcggt atggggccgg tggtttcggg gtgcgccgag cgacaggaag
     3601 aaccggtcgt tgccttggtg aatggacgag ccatcactca agccgaattc gaccttcggt
     3661 ggggtgaact ctccaaggcc acacgctcac gctatgagaa ggagggtggg aagcgaagat
     3721 tcctggacga gctcatcacc cgagagttgc tgatgcagga ggcccgtcgc cgaggtcttg
     3781 atcaggacga tacgattcgg gacaagacac agcggtataa agaacagctg attctcgatg
     3841 aactgttgaa agacaaactt caatccaagg tcgaagtcac ccaggccgaa ctcgatgcct
     3901 actacgaaaa gcacgccaac caactattgg accccttgaa ggccgatgtc tcgttgatgc
     3961 tcctccctaa tgtatatgcc gccagagacc ttgaagcaca gatgcataga ggcgggagtt
     4021 tcgtcaagtt tgcgcagcga tactcgatcg acgaaaagac gaaacacaag ggcggcgagc
     4081 tcggacccta ccggaaggga ctggttcccc cggaagtcga tgccgtcatc cacacgctca
     4141 aaattgggat ggtcagtgca ccgattaaga ccgacaacgg ctattacttg gtcatgctga
     4201 ccccgctgga cgaagccatt atccaagccg acttggcgac gcaggaacgg ctccgacaag
     4261 aattactgtc cgataagcgc cgcaagcggt tcgatgaaga catcgccgat attcgggcaa
     4321 atgccgtggt tcgcttcgcc ggagcctccc gtcatatcac cgaggacacc ggtaaacgct
     4381 agcctggctc gattccacag atttatctat ttcgtgtaaa atggtccgct cttgcgctat
     4441 ccggtgaaag gatcatgaaa cgcgtttcgc tccgatcgga tcggccggtc cggttcaagt
     4501 tgccgttagt cttgttggtc atcacgacct ggctcccggc catttccgag gcccgtttgc
     4561 aagatcgcat cgtcgccatc gtcaattccg agttgatcat gttgtcggat gtgaagcgcg
     4621 agtttgaaac ggagcacgag cggctttccc gcgagcaccg tggaaacgat ctccctcagc
     4681 gattgaaaac agcggagtac atggccttga cgaagctcat cgagcggc
//
LOCUS       DATNDD010000483         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_3435707, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000483 DATNDD010000000
VERSION     DATNDD010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435707"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..818
                     /locus_tag="VN647_11615"
     CDS             93..818
                     /locus_tag="VN647_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWG97723.1"
                     /translation="MSDVASRVLEVQSLRRSFFSNGVETNVLNGIDFFVQQGEYLAIS
                     GPSGSGKSTLLAILGLLDAVFTGTYLFNGYDVAALSGKERTRIRNQEIGFIFQEFNLV
                     ADLTVQENVELPLTYRDGISAKLCRQQARDSLKRVGMQHDLNHYPSELSGGQQQRVAV
                     ARALAGSPSLLLADEPTGNLDSVSGHTVMDLLSELNREGTTICLVTHNREFAARAKRV
                     IEIADGRIKNRADFYCDQKLNAD"
     gene            complement(797..1948)
                     /locus_tag="VN647_11620"
     CDS             complement(797..1948)
                     /locus_tag="VN647_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97724.1"
                     /translation="MAIAYGFGQSGTPKTIIRAGAGVFYERFALPNIMTTVRQNGMNQ
                     LQLLVTDPSRACNPTNVAACTGGTSGNTTYLTAPSLRTPYALMFAVEADQQIVRGVTL
                     SVNFRQTRGIHQFYSENLNAPQNGVYPVPPNGSAAPEVLYRFESGGIFNQSRLDFNIN
                     ARIHRYLSLYGYTFLNFARSDTGGPSTFPSVPGDIGADYGRGLFDIRQRSYLGGSASL
                     PFGLTWSFTLVGWSGSPYNVTLGSDLNKDSIYNDRPSFLPGENKASCRDAASFESPTS
                     GSRYVPIPINYCTGPGSFVAYTRLVKNFGIGPMRGSHKSSGRGSDTGRRYNFSIGVQF
                     QNLFNNADLSTPQGVLTSPQFGRSTQLAGNPFTASDSVRRIYLQSAFSF"
BASE COUNT          542 a          521 c          589 g          544 t
ORIGIN      
        1 gcggttctta tattgttccg aagttccggc catgacgtag acacactaca tcattgtcgt
       61 tgtattgtct aggagggagg ctcagcgaat caatgtctga tgtggcatca cgtgtgctcg
      121 aggtgcagtc tctgcgaagg agctttttca gcaatggtgt ggaaaccaac gtgctgaatg
      181 ggatcgactt ttttgtccag caaggcgaat acttggcgat ctcggggcca tctgggagtg
      241 gaaagtcgac actcttggca attttgggtc ttttggacgc agtcttcacc ggcacctatc
      301 tgttcaatgg gtatgatgtt gccgccctct cgggcaaaga acgtacacgc atcagaaatc
      361 aggaaatcgg ttttatcttc caggaattca acttagtagc tgatcttacc gttcaggaga
      421 acgtcgaatt gccgcttact tatcgagacg ggatttcggc gaaattgtgc cgccagcagg
      481 cccgcgactc gttgaaacgg gtcggcatgc aacacgatct gaaccattac ccttccgagc
      541 tttccggagg gcaacagcaa cgcgtcgccg ttgcgcgggc attggctggt tcaccctctt
      601 tactcctcgc cgacgaacca acgggtaatc ttgactctgt tagcgggcat acggtcatgg
      661 atttgctgtc tgaattgaat cgtgaaggca caacgatatg cttggtcact cacaatcgag
      721 aattcgctgc aagagctaag agagtcatcg agatcgccga tggcagaata aaaaatcggg
      781 ctgactttta ctgtgatcaa aaactgaatg cggattgaag atagatcctc cgtaccgagt
      841 cgctggcagt gaatggattt cctgccaatt gcgtggatct accgaactgg ggtgaggtga
      901 gtacgccctg cggcgtggac aggtcagcgt tattgaagag gttttggaat tggacaccaa
      961 tggaaaagtt atagcgtcgt cctgtatccg aaccccgccc cgacgattta tgggaccctc
     1021 gcatgggccc aataccgaag ttctttacca atcgtgtata cgccacaaag gaaccagggc
     1081 cggtgcagta atttatcggt ataggaacat acctagatcc cgatgttggg ctttcaaagc
     1141 tggcggcatc tcgacaactg gccttgttct cgcccggcag aaatgaaggc cgatcgttat
     1201 agatgctgtc tttattcagg tcgcttccta atgtgacgtt gtatggactt ccagaccaac
     1261 cgacaagagt aaagctccag gtaagcccaa atggaagcga agcgcttcct cccagatagc
     1321 tcctctgtct gatatcgaac agcccacgac catagtctgc gcctatatcg ccggggactg
     1381 aaggaaatgt actcggtcct cctgtatctg accgggcaaa attgagaaat gtatatccgt
     1441 aaagcgacag atatctgtga atccttgcat tgatgttgaa gtccaagcga ctctgattaa
     1501 atatgccccc ggattcaaac cgatagagca cctcaggagc tgccgatccg ttaggaggca
     1561 cagggtaaac accattttgt ggtgcgttta gattctcgct ataaaattga tgaattcccc
     1621 ttgtctgtct gaaatttacc gacaaagtca ctccacgaac gatctgctga tcagcttcta
     1681 ccgcaaacat tagcgcataa ggagtccgga gagatggggc agtgaggtat gtagtattgc
     1741 cggaggtgcc tcctgtacag gcggcaacgt ttgtgggatt acacgcacga ctgggatcag
     1801 tcaccaacaa ttgaagttga ttcattccgt tttgacggac agtggtcatg atattgggaa
     1861 gggcgaagcg ctcatagaac acaccggcac cggcacgaat gattgtcttc ggggtacctg
     1921 attggccaaa gccataggca atagccactc ggggtgcaaa gtcatggtga tcgcgaagat
     1981 gattctgggt ttcgtaccga agcccgtaac ttatgtcgag gcgtggtgta ggtttccatt
     2041 catcctcagc atataggccc aagtcgacta gggtcgcact gatggggcgg ttctcggttg
     2101 tgatactgaa ttgcgatgct tgaccagact cgtacgatga agatgtcccg ctagagtcga
     2161 ctgggcacgg agaaattacg gtattcgcaa tacagt
//
LOCUS       DATNDD010000484         4072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U11233 k141_2835309, whole
            genome shotgun sequence.
ACCESSION   DATNDD010000484 DATNDD010000000
VERSION     DATNDD010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945806
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 23:28:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,330
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835309"
                     /isolate="SMAG_U11233"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN647_11625"
     CDS             complement(<1..359)
                     /locus_tag="VN647_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWG97725.1"
                     /translation="MIQCRLMVPESTSDVSSFRVTFFFGPDSVEGHPDVLACVFNVKK
                     RSWKAGVQVSVEVSTDQLSGLRQKIRLADRLAKSLTAIDPREGSHYQERIADFFAQAV
                     CWCKLDLRLQAGLAQEN"
     gene            425..1627
                     /locus_tag="VN647_11630"
     CDS             425..1627
                     /locus_tag="VN647_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWG97726.1"
                     /translation="MSGVAHQTRVIAIQEALRNMGTVDGWLFYDFRGSDPLAYRVLLL
                     DPTQHVTRRWYYWIPTSGEPVKLLHRIEPHTLDELPGQCQPYVSWEQQRLLLASLLRG
                     RRRIAIQYSPFNAIPYLSRVDAGTVELIRSFGLEVVSSADLVQRFEAVWTDEQLESHR
                     YAVTALRKIVDEAFAYVASCLTNQRSLSEYGLQQFILSRIRDAGMTTSSAPIAAVDAH
                     SADPHYGPTESGSSLITRDNLVLIDLWAKQMAPGSVYGDITWTGYTGKQVPADQRMIF
                     EHVRRGRDAALSFVQDQVARGRFPCGWEVDDICRNKIRAAGYGDFFIHRTGHSIGEEV
                     HGNGANIDNLETQDSRRLMPKTCFSIEPGIYLPGEFGIRSELDVYIADREAIVHGLPL
                     QTEIISLL"
     gene            1711..1863
                     /gene="tatA"
                     /locus_tag="VN647_11635"
                     /pseudo
     CDS             1711..1863
                     /gene="tatA"
                     /locus_tag="VN647_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(2628..3146)
                     /locus_tag="VN647_11640"
     CDS             complement(2628..3146)
                     /locus_tag="VN647_11640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97727.1"
                     /translation="MTFPVVSTASHQGKYLVVDPGRETINVHDTSGQLLGNMSWGAII
                     ERVLVSGEDARFAHCRTQPRAPLALKVRYTTPEGKQFDSLTGGIGGGGLFIESGAPLS
                     PGTELTVEFALPDRPWDKLKAKAKVAWVRNKPERYLLFPGMGIQFVELDKKVQEQLVG
                     LVEALNLSRNPS"
     gene            complement(3196..3714)
                     /locus_tag="VN647_11645"
     CDS             complement(3196..3714)
                     /locus_tag="VN647_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWG97728.1"
                     /translation="MKYPVSSSLRHNGKVIELDVGREMIVLLGVSGDVIGSLSWDFVI
                     DQILAYRKVSVQKEVRLEPRISLAFRVRYNSPEGLRFESRAGGIGGGGLFIESHSPLP
                     VGTRLAMEFSFLEKSEEWMPAKGTVAWICPKADQYTFSPGMGVRFTEIAEDVRDRIHE
                     LVKTIQNMSQAA"
     gene            complement(3890..>4072)
                     /locus_tag="VN647_11650"
     CDS             complement(3890..>4072)
                     /locus_tag="VN647_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWG97729.1"
                     /translation="YAQKTGARGLRAILEEVMLDVMYDAPSQKQIVEVVITEEAILGK
                     NPPMRIFEHEKDAKTA"
BASE COUNT          966 a         1152 c         1026 g          928 t
ORIGIN      
        1 tgattttctt gggccagacc cgcctgcagc ctgagatcga gtttgcacca acaaacagct
       61 tgtgcaaaga aatccgctat gcgttcttga tagtgagagc cctcgcgggg gtcgattgcc
      121 gtcaaactct tcgcgagtcg atcagcaagg cgtattttct gccgaagacc cgacagttga
      181 tcggtgctca cctcgacaga gacctgaacg cccgctttcc aactccgttt tttcacgttg
      241 aacacacagg ccagcacgtc gggatgtccc tcaaccgagt caggcccgaa gaagaacgtg
      301 acgcgaaaag atgagacgtc actggttgat tctggaacca tgaggcggca ttgtatcatg
      361 attcacaggg agacttgggg gcttgattga cgtcgctgta ccccgaagat aagatggaaa
      421 caccatgagc ggcgtcgccc accaaacgcg ggtcattgcg atccaagagg cactccgaaa
      481 catggggact gtagatggat ggcttttcta cgactttcgc ggcagtgacc cgcttgccta
      541 tcgtgttttg ctccttgatc cgacccagca tgtcactcga cgctggtact attggatccc
      601 tacgtcggga gaaccggtca aactccttca caggatcgaa ccacatacgc tggatgagtt
      661 acccggtcaa tgccagccgt acgtctcatg ggaacagcaa cgtctgttat tggcctctct
      721 gttgcgaggt cggcggcgca tcgccataca atactcgccg ttcaacgcta ttccatacct
      781 gtccagagtt gatgctggca ccgtcgaact cattcgaagt tttggactcg aggtggtctc
      841 atcagccgat cttgtccaac gattcgaggc agtctggacg gatgagcaat tggaatccca
      901 tcggtacgcc gtgacggcac tcagaaagat tgtcgacgag gccttcgcct atgtggcgtc
      961 ttgtctcacc aaccaacggt cgttatctga atatggtcta caacagttca ttctctcacg
     1021 tattcgcgat gcgggcatga cgacatcgag tgctccgatt gcagctgttg atgcccatag
     1081 tgctgatccc cattatgggc cgactgagtc aggctcttca ctgatcacgc gagacaattt
     1141 ggttctgatc gatttatggg cgaaacaaat ggcaccgggc tcggtctatg gagatatcac
     1201 ctggacaggc tataccggga agcaggtgcc ggcagaccag cgcatgatct ttgaacacgt
     1261 tcggcgtggg cgtgatgcgg ccctatcctt cgtccaggat caggtggcta gaggccgatt
     1321 tccttgcgga tgggaagttg atgatatctg ccgtaataag attcgagcgg cgggctacgg
     1381 cgacttcttc atccatcgaa ccggccactc aatcggtgag gaagtccatg gtaatggagc
     1441 caatatcgac aacctggaaa ctcaggacag tcgtcggctc atgcccaaaa cttgcttttc
     1501 tatcgagccg ggaatctacc tcccgggaga gtttgggatt aggagcgagc ttgacgtcta
     1561 tatagcggat cgagaagcca tcgtgcacgg cttaccactt caaaccgaaa tcatatctct
     1621 gctctgaccc tttcttgaca ccatgtctga tgggcggcta gatttactca tccggcaaaa
     1681 atgccttcct aatcacatac gaggccgtac gtgttcggca caatgggttt ctccgaactc
     1741 attatcattt tggtcatcgt gctgattatt ttcggagcag gccgcctgcc gcagataggc
     1801 gaaggtgtcg gcaaggcatt gaaagggttt agaaaagaag tcaacgaagg gccccaatcc
     1861 gctccaggtc aggaccagca acaaaccgag cagccacaag ccattcacat tccaacgagc
     1921 attgcagtga cacagtctct gaccgcagga tcatcgccgg ccacggccag gccaaacact
     1981 ccccatgtcc ctggtccgga actcacgccc ggcaccaccg cctccttgtt gtacaataca
     2041 agccttcaga cttcgcgagc atcggctcct tccccaaatc aagcatcgac agctgcctcc
     2101 aacccggtgc tatcgatgga ggaacgggct gccgcttccc ctccaagggc tcatgcctcg
     2161 tatccacctt tgccgaccgg ctcccaagca aaagcgacgg ccaaacgtcc ttcagccatc
     2221 gtcaataagg acgcggttgc ccgtgtgcaa gcacaacaag ctgccctcaa agctaagcag
     2281 gcaccatcag tggttggagt atcgcccgat gatctgcagc atctcggtga aggtctcgga
     2341 gaggtcgtgc gaacgttccg gcaaaccgtg accgatgttc gcaatagcgt ggatcctcaa
     2401 atgtgtacga ttcagggcga aatggaggca gccaaaaagg aaatccagca atccattgaa
     2461 gcagctaaag cactaccacc ggtgcaagag aagcctccga aatctgcgtg attctcatat
     2521 caacggctct ccgcatttct ctcaaatcct tgccgaccga tgtgtgccca cttcgcacca
     2581 ctgccataca ggcttgttgt cgctataggt aagcgagcct attctgatca agaagggttt
     2641 cgacttagat tcaaagcctc aaccaggccg accaattgct cctgaacctt cttatccaac
     2701 tccacgaact gaatgcccat gcctggaaac aaaagatatc gctccggctt gttgcggacc
     2761 caggcgacct tcgccttggc tttcaacttg tcccacggtc gatccggaag cgcaaattcg
     2821 accgtcagct cggtgcctgg actgagagga gcaccactct cgataaagag cccgccgccg
     2881 ccgattcctc cggttaggct atcaaactgc tttccttctg gcgtcgtata tcggaccttc
     2941 agggccagag gcgcacgggg ctgcgtacga caatgggcaa atcgggcatc ttcaccgctg
     3001 acaagtactc gctcgataat tgcgccccat gacatgttgc ctaaaagctg gcccgaggtg
     3061 tcgtgaacgt tgatcgtctc tcgccccgga tcaacaacga gatatttccc ctgatgactc
     3121 gcagtggaaa cgacgggaaa cgtcataaaa ccatcccttt ccctttgggg ggcgggccca
     3181 agtcatgaac ggaaactagg ctgcttgact catgttctgg attgttttca ctaactcgtg
     3241 gattctatcg cggacatcct ccgctatctc agtgaaccgt acccccattc caggactgaa
     3301 ggtatattga tcggcttttg gacagatcca tgctacggtt ccttttgccg gcatccattc
     3361 ctcggatttc tccagaaatg agaactccat agccaatctg gtccctacag gaagagggga
     3421 atggctttca ataaataagc ctccgccacc aataccacct gctcgacttt caaatcgcag
     3481 cccctctggg ctgttgtaac gcacacggaa tgcaagggaa atgcgaggct ctaaccggac
     3541 ttccttctga acagatacct tgcggtaggc gaggatttga tcgatgacga agtcccagga
     3601 caagctgcct attacatccc cactgacgcc caacaagacg atcatttcac gtcccacgtc
     3661 aagttcgatg actttgccat tgtgccgaag actcgatgaa acagggtact tcacaagtcc
     3721 tccatccaag acacgcacga ggcaagtata gcgcggcgtt tccgattgtc gaggaagatg
     3781 agagctgttg aacgaagaga gggaaagcta ccggcaggaa gcgggtcgat tcgatccccc
     3841 tacgagtaat tagctagggc catcgattat tcatggaatt tggacaattt tacgcggtct
     3901 tagcgtcctt ttcatgttca aatatgcgca tcggtgggtt tttgcccaag attgcctctt
     3961 ccgtaattac cacctcgacg atctgtttct gagagggggc atcgtacatg acatcaagca
     4021 tcacttcttc caggattgcc cgaaggcctc tggcaccagt cttctgcgca ta
//
